An antiviral antibiotic produced by Cephalosporium aphidicola and other fungi. It inhibits the growth of eukaryotic cells and certain animal viruses by selectively inhibiting the cellular replication of DNA polymerase II or the viral-induced DNA polymerases. The drug may be useful for controlling excessive cell proliferation in patients with cancer, psoriasis or other dermatitis with little or no adverse effect upon non-multiplying cells.
All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.
Twenty-carbon compounds derived from MEVALONIC ACID or deoxyxylulose phosphate.
The study of natural phenomena by observation, measurement, and experimentation.
A DNA-dependent DNA polymerase characterized in E. coli and other lower organisms. It may be present in higher organisms and has an intrinsic molecular activity only 5% of that of DNA Polymerase I. This polymerase has 3'-5' exonuclease activity, is effective only on duplex DNA with gaps or single-strand ends of less than 100 nucleotides as template, and is inhibited by sulfhydryl reagents. EC 2.7.7.7.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
A generic concept reflecting concern with the modification and enhancement of life attributes, e.g., physical, political, moral and social environment; the overall condition of a human life.
A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.
A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.
Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A simple organophosphorus compound that inhibits DNA polymerase, especially in viruses and is used as an antiviral agent.
DNA-dependent DNA polymerases found in bacteria, animal and plant cells. During the replication process, these enzymes catalyze the addition of deoxyribonucleotide residues to the end of a DNA strand in the presence of DNA as template-primer. They also possess exonuclease activity and therefore function in DNA repair.
A genus of the family HERPESVIRIDAE, subfamily ALPHAHERPESVIRINAE, consisting of herpes simplex-like viruses. The type species is HERPESVIRUS 1, HUMAN.
A group of acute infections caused by herpes simplex virus type 1 or type 2 that is characterized by the development of one or more small fluid-filled vesicles with a raised erythematous base on the skin or mucous membrane. It occurs as a primary infection or recurs due to a reactivation of a latent infection. (Dorland, 27th ed.)
The type species of SIMPLEXVIRUS causing most forms of non-genital herpes simplex in humans. Primary infection occurs mainly in infants and young children and then the virus becomes latent in the dorsal root ganglion. It then is periodically reactivated throughout life causing mostly benign conditions.
Deoxyribonucleic acid that makes up the genetic material of viruses.
The posterior of the three primitive cerebral vesicles of an embryonic brain. It consists of myelencephalon, metencephalon, and isthmus rhombencephali from which develop the major BRAIN STEM components, such as MEDULLA OBLONGATA from the myelencephalon, CEREBELLUM and PONS from the metencephalon, with the expanded cavity forming the FOURTH VENTRICLE.
A family of DNA-binding transcription factors that contain a basic HELIX-LOOP-HELIX MOTIF.
Formation of NEURONS which involves the differentiation and division of STEM CELLS in which one or both of the daughter cells become neurons.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
The middle of the three primitive cerebral vesicles of the embryonic brain. Without further subdivision, midbrain develops into a short, constricted portion connecting the PONS and the DIENCEPHALON. Midbrain contains two major parts, the dorsal TECTUM MESENCEPHALI and the ventral TEGMENTUM MESENCEPHALI, housing components of auditory, visual, and other sensorimoter systems.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism.
Recurring supersecondary structures characterized by 20 amino acids folding into two alpha helices connected by a non-helical "loop" segment. They are found in many sequence-specific DNA-BINDING PROTEINS and in CALCIUM-BINDING PROTEINS.
Former state in north central Germany. Formally abolished March 1, 1947. Kingdom established 1701.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
The study of the structure, preparation, properties, and reactions of carbon compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Sugars in which the OXYGEN is replaced by a NITROGEN atom. This substitution prevents normal METABOLISM resulting in inhibition of GLYCOSIDASES and GLYCOSYLTRANSFERASES.
Organic compounds containing the carboxy group (-COOH). This group of compounds includes amino acids and fatty acids. Carboxylic acids can be saturated, unsaturated, or aromatic.
Synthetic organic reactions that use reactions between unsaturated molecules to form cyclical products.
The ordered rearrangement of gene regions by DNA recombination such as that which occurs normally during development.
One of the cytotoxic dihalohexitols that may have alkylating antineoplastic activity. It causes bone marrow toxicity. The active form is thought to be the diepoxide.
Photochemotherapy using visible light, usually red, topically or delivered locally by fiberoptic probe to tissues sensitized with hematoporphyrins.
The relationship between the dose of administered radiation and the response of the organism or tissue to the radiation.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Discussion of lists of works, documents or other publications, usually with some relationship between them, e.g., by a given author, on a given subject, or published in a given place, and differing from a catalog in that its contents are restricted to holdings of a single collection, library, or group of libraries. (from The ALA Glossary of Library and Information Science, 1983)
The outer of the three germ layers of an embryo.
The commonest and widest ranging species of the clawed "frog" (Xenopus) in Africa. This species is used extensively in research. There is now a significant population in California derived from escaped laboratory animals.
The region in the dorsal ECTODERM of a chordate embryo that gives rise to the future CENTRAL NERVOUS SYSTEM. Tissue in the neural plate is called the neuroectoderm, often used as a synonym of neural plate.
A tube of ectodermal tissue in an embryo that will give rise to the CENTRAL NERVOUS SYSTEM, including the SPINAL CORD and the BRAIN. Lumen within the neural tube is called neural canal which gives rise to the central canal of the spinal cord and the ventricles of the brain. For malformation of the neural tube, see NEURAL TUBE DEFECTS.
The developmental entity of a fertilized egg (ZYGOTE) in animal species other than MAMMALS. For chickens, use CHICK EMBRYO.
An aquatic genus of the family, Pipidae, occurring in Africa and distinguished by having black horny claws on three inner hind toes.
Proteins obtained from various species of Xenopus. Included here are proteins from the African clawed frog (XENOPUS LAEVIS). Many of these proteins have been the subject of scientific investigations in the area of MORPHOGENESIS and development.
Specific loci that show up during KARYOTYPING as a gap (an uncondensed stretch in closer views) on a CHROMATID arm after culturing cells under specific conditions. These sites are associated with an increase in CHROMOSOME FRAGILITY. They are classified as common or rare, and by the specific culture conditions under which they develop. Fragile site loci are named by the letters "FRA" followed by a designation for the specific chromosome, and a letter which refers to which fragile site of that chromosome (e.g. FRAXA refers to fragile site A on the X chromosome. It is a rare, folic acid-sensitive fragile site associated with FRAGILE X SYNDROME.)
Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.
Databases devoted to knowledge about specific genes and gene products.
The complete genetic complement contained in the DNA of a set of CHROMOSOMES in a HUMAN. The length of the human genome is about 3 billion base pairs.
Susceptibility of chromosomes to breakage leading to translocation; CHROMOSOME INVERSION; SEQUENCE DELETION; or other CHROMOSOME BREAKAGE related aberrations.
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.

Involvement of p21 in the PKC-induced regulation of the G2/M cell cycle transition. (1/594)

Activation of protein kinase C (PKC) inhibits cell cycle progression at the G1/S and G2/M transitions. We found that phorbol 12-myristate 13-acetate (PMA) induced upregulation of p21, not only in MCF-7 cells arrested in the G1 phase as previously shown, but also in cells delayed in the G2 phase. This increase in p21 in cells accumulated in the G1 and G2/M phases of the cell cycle after PMA treatment was inhibited by the PKC inhibitor GF109203X. This indicates that PKC activity is required for PMA-induced p21 upregulation and cell cycle arrest in the G1 and G2/M phases of the cell cycle. To further assess the role of p21 in the PKC-induced G2/M cell cycle arrest independently of its G1 arrest, we used aphidicolin-synchronised MCF-7 cells. Our results show that, in parallel with the inhibition of cdc2 activity, PMA addition enhanced the associations between p21 and either cyclin B or cdc2. Furthermore, we found that after PMA treatment p21 was able to associate with the active Tyr-15 dephosphorylated form of cdc2, but this complex was devoid of kinase activity indicating that p21 may play a role in inhibition of cdc2 induced by PMA. Taken together, these observations provide evidence that p21 is involved in integrating the PKC signaling pathway to the cell cycle machinery at the G2/M cell cycle checkpoint.  (+info)

Cell cycle-dependent nuclear accumulation of the p94fer tyrosine kinase is regulated by its NH2 terminus and is affected by kinase domain integrity and ATP binding. (2/594)

p94fer and p51ferT are two tyrosine kinases that are encoded by differentially spliced transcripts of the FER locus in the mouse. The two tyrosine kinases share identical SH2 and kinase domains but differ in their NH2-terminal amino acid sequence. Unlike p94fer, the presence of which has been demonstrated in most mammalian cell lines analyzed, the expression of p51ferT is restricted to meiotic cells. Here, we show that the two related tyrosine kinases also differ in their subcellular localization profiles. Although p51ferT accumulates constitutively in the cell nucleus, p94fer is cytoplasmic in quiescent cells and enters the nucleus concomitantly with the onset of S phase. The nuclear translocation of the FER proteins is driven by a nuclear localization signal (NLS), which is located within the kinase domain of these enzymes. The functioning of that NLS depends on the integrity of the kinase domain but was not affected by inactivation of the kinase activity. The NH2 terminus of p94fer dictated the cell cycle-dependent functioning of the NLS of FER kinase. This process was governed by coiled-coil forming sequences that are present in the NH2 terminus of the kinase. The regulatory effect of the p94fer NH2-terminal sequences was not affected by kinase activity but was perturbed by mutations in the kinase domain ATP binding site. Ectopic expression of the constitutively nuclear p51ferT in CHO cells interfered with S-phase progression in these cells. This was not seen in p94fer-overexpressing cells. The FER tyrosine kinases seem, thus, to be regulated by novel mechanisms that direct their different subcellular distribution profiles and may, consequently, control their cellular functioning.  (+info)

Nucleo-cytoplasmic interactions that control nuclear envelope breakdown and entry into mitosis in the sea urchin zygote. (3/594)

In sea urchin zygotes and mammalian cells nuclear envelope breakdown (NEB) is not driven simply by a rise in cytoplasmic cyclin dependent kinase 1-cyclin B (Cdk1-B) activity; the checkpoint monitoring DNA synthesis can prevent NEB in the face of mitotic levels of Cdk1-B. Using sea urchin zygotes we investigated whether this checkpoint prevents NEB by restricting import of regulatory proteins into the nucleus. We find that cyclin B1-GFP accumulates in nuclei that cannot complete DNA synthesis and do not break down. Thus, this checkpoint limits NEB downstream of both the cytoplasmic activation and nuclear accumulation of Cdk1-B1. In separate experiments we fertilize sea urchin eggs with sperm whose DNA has been covalently cross-linked to inhibit replication. When the pronuclei fuse, the resulting zygote nucleus does not break down for >180 minutes (equivalent to three cell cycles), even though Cdk1-B activity rises to greater than mitotic levels. If pronuclear fusion is prevented, then the female pronucleus breaks down at the normal time (average 68 minutes) and the male pronucleus with cross-linked DNA breaks down 16 minutes later. This male pronucleus has a functional checkpoint because it does not break down for >120 minutes if the female pronucleus is removed just prior to NEB. These results reveal the existence of an activity released by the female pronucleus upon its breakdown, that overrides the checkpoint in the male pronucleus and induces NEB. Microinjecting wheat germ agglutinin into binucleate zygotes reveals that this activity involves molecules that must be actively translocated into the male pronucleus.  (+info)

The Drosophila ATM homologue Mei-41 has an essential checkpoint function at the midblastula transition. (4/594)

BACKGROUND: Drosophila embryogenesis is initiated by 13 rapid syncytial mitotic divisions that do not require zygotic gene activity. This maternally directed cleavage phase of development terminates at the midblastula transition (MBT), at which point the cell cycle slows dramatically, membranes surround the cortical nuclei to form a cellular blastoderm, and zygotic gene expression is first required. RESULTS: We show that embryos lacking Mei-41, a Drosophila homologue of the ATM tumor suppressor, proceed through unusually short syncytial mitoses, fail to terminate syncytial division following mitosis 13, and degenerate without forming cells. A similar cleavage-stage arrest is produced by mutations in grapes, which encodes a homologue of the Checkpoint-1 kinase. We present biochemical, cytological and genetic data indicating that Mei-41 and Grapes are components of a conserved DNA-replication/damage checkpoint pathway that triggers inhibitory phosphorylation of the Cdc2 kinase and mediates resistance to replication inhibitors and DNA-damaging agents. This pathway is nonessential during postembryonic development, but it is required to terminate the cleavage stage at the MBT. Cyclins are required for Cdc2 kinase activity, and mutations in cyclin A and cyclin B bypass the requirement for mei-41 at the MBT. These mutations do not restore wild-type syncytial cell-cycle timing or the embryonic replication checkpoint, however, suggesting that Mei-41-mediated inhibition of Cdc2 has an additional essential function at the MBT. CONCLUSIONS: The Drosophila DNA-replication/damage checkpoint pathway can be activated by externally triggered DNA damage or replication defects throughout the life cycle, and under laboratory conditions this inducible function is nonessential. During early embryogenesis, however, this pathway is activated by developmental cues and is required for the transition from maternal to zygotic control of development at the MBT.  (+info)

Modulation of drug resistance mediated by loss of mismatch repair by the DNA polymerase inhibitor aphidicolin. (5/594)

Loss of expression of mismatch repair (MMR) proteins leads to resistance of tumor cells to a variety of DNA-damaging agents, including bifunctional alkylating and monofunctional methylating agents such as cis-diaminedichloroplatinum II (CDDP) and N'-methyl-N-nitrosourea (MNU). It has been suggested that coupling to cell death does not occur in the absence of MMR, but instead, DNA lesions are bypassed during replication, giving a drug-tolerant phenotype. In the present study, we have used aphidicolin (Ap), an inhibitor of DNA polymerases, to study the role of replicative bypass in drug resistance mediated by loss of MMR. We have examined the survival of matched ovarian carcinoma cell lines with known MMR status after sequential treatment with CDDP or MNU and Ap. We show that Ap increases the sensitivity of MMR-deficient cell lines to CDDP and MNU to a greater extent than their MMR-proficient counterparts. Furthermore, loss of MMR correlates with loss of CDDP-induced G2 arrest, but this is partially restored after Ap treatment. These data support Ap sensitizing drug-resistant cancer cells that have lost MMR to CDDP and MNU and suggest that the potential use of Ap as a modulator of drug resistance should be targeted to MMR-defective tumors.  (+info)

Developmental activation of the capability to undergo checkpoint-induced apoptosis in the early zebrafish embryo. (6/594)

In this study, we demonstrate the developmental activation, in the zebrafish embryo, of a surveillance mechanism which triggers apoptosis to remove damaged cells. We determine the time course of activation of this mechanism by exposing embryos to camptothecin, an agent which specifically inhibits topoisomerase I within the DNA replication complex and which, as a consequence of this inhibition, also produces strand breaks in the genomic DNA. In response to an early (pre-gastrula) treatment with camptothecin, apoptosis is induced at a time corresponding approximately to mid-gastrula stage in controls. This apoptotic response to a block of DNA replication can also be induced by early (pre-MBT) treatment with the DNA synthesis inhibitors hydroxyurea and aphidicolin. After camptothecin treatment, a high proportion of cells in two of the embryo's three mitotic domains (the enveloping and deep cell layers), but not in the remaining domain (the yolk syncytial layer), undergoes apoptosis in a cell-autonomous fashion. The first step in this response is an arrest of the proliferation of all deep- and enveloping-layer cells. These cells continue to increase in nuclear volume and to synthesize DNA. Eventually they become apoptotic, by a stereotypic pathway which involves cell membrane blebbing, "margination" and fragmentation of nuclei, and cleavage of the genomic DNA to produce a nucleosomal ladder. Fragmentation of nuclei can be blocked by the caspase-1,4,5 inhibitor Ac-YVAD-CHO, but not by the caspase-2,3,7[, 1] inhibitor Ac-DEVD-CHO. This suggests a functional requirement for caspase-4 or caspase-5 in the apoptotic response to camptothecin. Recently, Xenopus has been shown to display a developmental activation of the capability for stress- or damaged-induced apoptosis at early gastrula stage. En masse, our experiments suggest that the apoptotic responses in zebrafish and Xenopus are fundamentally similar. Thus, as for mammals, embryos of the lower vertebrates exhibit the activation of surveillance mechanisms, early in development, to produce the selective apoptosis of damaged cells.  (+info)

Cell-cycle perturbation in Sf9 cells infected with Autographa californica nucleopolyhedrovirus. (7/594)

Flow cytometry analysis of the cell-cycle progression was performed in Sf9 cells infected with Autographa californica nucleopolyhedrovirus (AcNPV) in the cultures partially synchronized by aphidicolin exposure and deprivation. Cells infected with AcNPV during the G1 phase progressed and were arrested in the S phase in the 4 h following the infection, whereas cells infected during the S phase did not progress past the S phase. Cells infected during the G2/M phase remained in the G2/M phase without mitosis during a period of 10 h. Such cell-cycle arrest was also observed in the cells infected with ts8, a temperature-sensitive mutant of AcNPV that is defective in both genomic DNA synthesis and late gene expression. Cells with >4 N DNA content accumulated in the cultures infected with wild-type AcNPV, whereas no such cells appeared in the cultures infected with ts8, suggesting that viral origin of the DNA overaccumulated in the cells with >4 N DNA content. This was confirmed by the slot blot hybridization experiments, which showed that viral DNA, but not cellular DNA, increased strikingly in Sf9 cells during the infection with AcNPV. These results indicate that AcNPV targets at least two different checkpoints to prevent normal cell-cycle progression of Sf9 cells and that neither viral DNA replication nor expression of viral late genes is a necessary prerequisite for such AcNPV-induced cell-cycle arrest. It is suggested that the cell-cycle arrest in AcNPV-infected Sf9 cells is an event triggered early in infection by specific interaction of viral gene products with cellular components that regulate cell-cycle progression.  (+info)

Antisense oligonucleotide complementary to the BamHI-H gene family of Marek's disease virus induced growth arrest of MDCC-MSB1 cells in the S-phase. (8/594)

DNA synthesis was effectively inhibited by antisense oligonucleotide A1 complementary to the BamHI-H gene family in Marek's disease virus (MDV)-derived lymphoblastoid MDCC-MSB1 cells. When a cell cycle distribution of a total cell population was analyzed by flow cytometry, the proportion of S-phase cells increased in the cell populations by treatment with oligonucleotide A1. Approximately 60-70% of the cells appeared in the S phase for 24 and 36 hr of incubation in the presence of oligonucleotide A1 (20-30% in the untreated control cells). The inhibition of cell cycle progression by treatment with oligonucleotide A1 was reversible. When the cells were treated with 5 microM aphidicolin for 12 hr, a similar pattern of cell cycle distribution was observed to that obtained after treatment with oligonucleotide A1. Aphidicolin is an inhibitor of cellular DNA polymerase alpha, and it halts progression of the cell cycle at the G1/S border or early S phase. When the cells were treated with aphidicolin for 12 hr and subsequently incubated with oligonucleotide A1, no significant difference was observed in the cycle phase distribution of cells in the presence and absence of oligonucleotide A1. In contrast, when the cells were treated with oligonucleotide A1 for 12 hr and subsequently incubated with aphidicolin, the cell cycle did not progress from the G1/S border or early S phase to the next phase.  (+info)

Seki, S; Oda, T; and Ohashi, M, Differential effects of aphidicolin on replicative dna synthesis and unscheduled dna synthesis in permeable mouse sarcoma cells. (1980). Subject Strain Bibliography 1980. 284 ...
Iliakis, G and Nusse, M, Aphidicolin promotes repair of potentially lethal damage in irradiated mammalian cell synchronized in s-phase. (1982). Subject Strain Bibliography 1982. 2980 ...
Shop a large selection of Signal Transduction Reagents and Kits products and learn more about Alfa Aesar Aphidicolin Quantity: 5mg:Life Sciences Quantity: 5mg.
Please provide some assistance generating ideas for the following questions: - What is a successful lesson framework composed of? How would this framework vary between two different content areas? - Why is it important to.
Summary Aphidicolin, a tetracyclic diterpenoid which inhibits the DNA polymerase-α activities of many eukaryotic cells, inhibited herpes simplex virus growth and DNA synthesis in infected cultures and the activity of the virus DNA polymerase in vitro. A wide range of stable aphidicolin sensitivities was represented amongst a collection of virus strains with no prior exposure to this drug, but viruses with polymerase mutations selected for resistance to phosphonoacetic acid (PAA) or to acycloguanosine typically showed increased sensitivity to aphidicolin. Of 16 unrelated PAA-resistant variants, 7 were hypersensitive to aphidicolin. A number of mutants with temperature-sensitive (ts) lesions in the polymerase gene also showed increased aphidicolin sensitivity (e.g. HSV-1[mP17]tsH) or aphidicolin hypersensitivity (e.g. HSV-1[KOS]tsD9, tsC4). Resistance or hypersensitivity of virus growth and DNA synthesis in vivo were correlated with resistance or hypersensitivity of virus DNA polymerase reactions in
Polo-like kinase 1 (Plk1) plays pivotal roles in mitosis; however, little is known about its function in S phase. In this study, we show that inhibition of Plk1 impairs DNA replication and results in slow S-phase progression in cultured cancer cells. We have identified origin recognition complex 2 (Orc2), a member of the DNA replication machinery, as a Plk1 substrate and have shown that Plk1 phosphorylates Orc2 at Ser188 in vitro and in vivo. Furthermore, Orc2-S188 phosphorylation is enhanced when DNA replication is under challenge induced by ultraviolet, hydroxyurea, gemcitabine, or aphidicolin treatment. Cells expressing the unphosphorylatable mutant (S188A) of Orc2 had defects in DNA synthesis under stress, suggesting that this phosphorylation event is critical to maintain DNA replication under stress. To dissect the mechanism pertinent to this observation, we showed that Orc2-S188 phosphorylation associates with DNA replication origin and that cells expressing Orc2-S188A mutant fail to ...
DNA synthesis in vitro in Brij-treated Saccharomyces cerevisiae requires the product of the CDC8 gene (Hereford, L. M. & Hartwell, L. H. (1971) Nature (London) New Biol. 234, 171-172). Extracts of wild-type A364a yeast restore DNA synthesis in Brij-treated cdc8, a mutant containing a thermolabile cdc8 gene product. This constitutes a complementation assay by which the cdc8 gene product can be monitored during purification. A heat-stable protein responsible for this complementation has been partially purified from both wild-type A364a cells and from a cdc8 temperature-sensitive mutant. The complementation activity from the mutant is thermolabile when compared to the wild-type activity, indicating that CDC8 is the structural gene for the protein. ...
Employing a novel strategy, we have virtually screened a large library of compounds to identify novel inhibitors of the reverse transcriptase (RT) of HIV-1. Fifty-six top scored compounds were tested in vitro, and two of them inhibited efficiently the DNA polymerase activity of RT. The most effective compound, N-{2
Active against HSV-1 HSV-2 (herpes simplex virus), EBV (ebstein barr virus), CMV (cytomegalo virus), HHV-6 (herpes human virus) & VZV (varicella zoster virus ...
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DNA replication is a highly demanding process regarding the energy and material supply and must be precisely regulated, involving multiple cellular feedbacks. The slowing down or stalling of DNA synthesis and/or replication forks is referred to as replication stress (RS). Owing to the complexity and requirements of replication, a plethora of factors may interfere and challenge the genome stability, cell survival or affect the whole organism. This review outlines chemical compounds that are known inducers of RS and commonly used in laboratory research. These compounds act on replication by direct interaction with DNA causing DNA crosslinks and bulky lesions (cisplatin), chemical interference with the metabolism of deoxyribonucleotide triphosphates (hydroxyurea), direct inhibition of the activity of replicative DNA polymerases (aphidicolin) and interference with enzymes dealing with topological DNA stress (camptothecin, etoposide). As a variety of mechanisms can induce RS, the responses of mammalian
Complete information for FRA6G gene (Uncategorized), Fragile Site, Aphidicolin Type, Common, Fra(6)(Q15), including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
InvivoGen provides a collection of inhibitors for different pathways: Signal Transduction Inhibitors, Protein Kinase inhibitors, Epigenetic Inhibitors, Heat Shock Protein Inhibitors, DNA Synthesis Inhibitors
TY - JOUR. T1 - Chromosomal aberrations and common fragile sites in neuroblastoma patients. AU - Vernole, P.. AU - Tedeschi, B.. AU - Pianca, C.. AU - Nicoletti, B.. AU - Riccardi, R.. AU - Melino, G.. PY - 1990. Y1 - 1990. N2 - We analyzed cytogenetically blood cells and bone marrow cells from 20 neuroblastoma patients. Chromosome common fragile sites were induced by aphidicolin in normal peripheral blood lymphocytes. All neuroblastoma patients showed a higher increase of aberrations after aphidicolin treatment as compared to that found in normal controls. In some cases it was possible to correlate the increase of the expression of a specific fragile site, 1p32, with deletions in the same area in bone marrow cells.. AB - We analyzed cytogenetically blood cells and bone marrow cells from 20 neuroblastoma patients. Chromosome common fragile sites were induced by aphidicolin in normal peripheral blood lymphocytes. All neuroblastoma patients showed a higher increase of aberrations after aphidicolin ...
High Molecular Weight DNA Markers from Invitrogen,High Molecular Weight DNA Markers are suitable for sizing linear double-stranded DNA from 9 to 48 kb on low-percentage agarose gels. The 13 bands consist of 8.3- to 48.5-kb fragments generated from restriction endonuclease digests of Lambda DNA (cIind1ts857 Sam7). Ethidium bromide staining causes t,biological,biology supply,biology supplies,biology product
Fragile sites are the chromosomal regions that are susceptible to breakage, and their frequency varies among the human population. Based on the frequency of fragile site induction, they are categorized as common and rare fragile sites. Common fragile sites are sensitive to replication stress and often rearranged in cancer. Rare fragile sites are the archetypal trinucleotide repeats. Fragile sites are known to be involved in chromosomal rearrangements in tumors. Human miRNA genes are also present at fragile sites. A better understanding of genes and miRNAs lying in the fragile site regions and their association with disease progression is required. HumCFS is a manually curated database of human chromosomal fragile sites. HumCFS provides useful information on fragile sites such as coordinates on the chromosome, cytoband, their chemical inducers and frequency of fragile site (rare or common), genes and miRNAs lying in fragile sites. Protein coding genes in the fragile sites were identified by mapping the
BioMed Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies covering a wide range of subjects in life sciences and medicine. The journal is divided into 55 subject areas.
TY - JOUR. T1 - FRA3B extends over a broad region and contains a spontaneous HPV16 integration site. T2 - Direct evidence for the coincidence of viral integration sites and fragile sites. AU - Wilke, Charles M.. AU - Hall, Bryan K.. AU - Hoge, Ann. AU - Paradee, William. AU - Smith, David I.. AU - Glover, Thomas W.. PY - 1996/2. Y1 - 1996/2. N2 - The common fragile site at 3p14.2 (FRA3B) is the most sensitive site on normal human chromosomes for the formation of gaps and breaks when DNA replication is perturbed by aphidicolin or folate stress. Although rare fragile sites are known to arise through the expansion of CCG repeats, the mechanism responsible for common fragile sites is unknown. Beyond being a basic component of chromosome structure, no biological effects of common fragile sites have been convincingly shown, although suggestions have been made that breakage and recombination at these sites may sometimes be mechanistically involved in deletions observed in many tumors and in ...
Discussion Enzymatically active DNA-PK is a central component of the cellular response to replication fork inhibition [6]. For instance, cells depleted of endogenous DNA-PKcs or expressing an enzymatically deficient kinase mutant are unable to efficiently restart stalled replication forks and show reduced clonogenic survival when treated with hydroxyurea [7]. With the notable exception of RPA32 [8], as well as of auto-phosphorylation [7], few relevant DNA-PK substrates have however been identified in response to replication stress. In this article we demonstrate that hSSB1 S134 phosphorylation is required for clonogenic survival of cells treated with the replication stress compounds hydroxyurea, aphidicolin and camptothecin, as well as establish that phosphorylation is primarily a result of DNA-PK activity. As a small decrease in hSSB1 S134 phosphorylation was also observed following the inhibition of ATM and ATR, we cannot however exclude that these kinases may also contribute a small amount to ...
V-58 is a novel inhibitor of Mtb replication within macrophages and stimulate cAMP production in Mtb, rapidly and directly activating Mtb AC Rv1625c to produce high levels of cAMP regardless of the bacterial environment or growth medium.
Chronic inflammation refers to a response by your immune system that sticks around long after an infection, injury, or exposure to a toxin. Well look at common symptoms, its role in various diseases, and the kinds of treatments that are available, from medications to anti-inflammatory diets.
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Excess Hsl7 overrides the DNA replication checkpoint. (A) Xenopus Hsl7 or β-globin mRNA was incubated in cycling extracts in the presence of aphidicolin (200
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Thymidine, a pyrimidine deoxynucleoside, is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication. - Mechanism of Action & Protocol.
Im wondering if the Hirt high molecular weight DNA prep has been replaced by a simpler or kit like method. I have to isolate a viral DNA from some infected tissue culture cells and would like to make it as painless as possible. Thanks for your help. Mary ...
Genomes and Genes, Locale, Publications, Species, Research Topics, Scientific Experts about Experts and Doctors on dna replication in United States
Guanazole and aphidicolin were chosen as candidates in the search for a selective, non-genotoxic inhibitor of DNA replication which could be used instead of hydroxyurea to measure DNA repair synthesis in rat hepatocyte primary cultures by liquid scintillation counting. The genotoxicity of these 3 chemicals was studied using the Salmonella/liver homogenate assay and the autoradiographic UDS test in hepatocytes. Hydroxyurea was positive in both of these assays. Guanazole and aphidicolin did not induce DNA repair in hepatocytes. Aphidicolin was not mutagenic for Salmonella typhimurium, whereas guanazole increased the revertant numbers of strain TA102 slightly. The incorporation of [3H]thymidine was measured by liquid scintillation to determine DNA repair induced by 2-acetylaminofluorene (2-AAF), aflatoxin B1, benzo[a]pyrene, cyclophosphamide, H2O2, 6-hydroxydopamine, N-methyl-N-nitro-N-nitrosoguanidine (MNNG), methylnitrosourea (MNU), 4-nitroquinoline-N-oxide and UV irradiation in the presence of ...
Open in another window Shape?1. Speculative style of Pol function in replication checkpoint. Upon replication fork stalling with replicative DNA polymerases inhibitors (hydroxyurea, aphidicolin) or UV-blocking lesions, ssDNA can be generated with the action from the helicase (CMG complicated). Replicative DNA polymerases and aswell as TLS polymerase Pol donate to synthesis and/or stabilization of little replication intermediates. These buildings are bound with the checkpoint clamp 9-1-1 complicated. DNA Pol may connect to the 9-1-1 complicated on chromatin, hence facilitating local development of the energetic ATR complicated including ATRIP and ToPBP1. Pol can also be implicated in replication fork restart by repriming (issue mark). Weve also observed that Pol downregulation in mammalian leads to deposition of DNA harm, thus uncovering a function for Pol during DNA replication in unperturbed cells and additional extending the function of the DNA polymerase outdoors TLS.4 Interestingly, in the ...
Open in another window Shape?1. Speculative style of Pol function in replication checkpoint. Upon replication fork stalling with replicative DNA polymerases inhibitors (hydroxyurea, aphidicolin) or UV-blocking lesions, ssDNA can be generated with the action from the helicase (CMG complicated). Replicative DNA polymerases and aswell as TLS polymerase Pol donate to synthesis and/or stabilization of little replication intermediates. These buildings are bound with the checkpoint clamp 9-1-1 complicated. DNA Pol may connect to the 9-1-1 complicated on chromatin, hence facilitating local development of the energetic ATR complicated including ATRIP and ToPBP1. Pol can also be implicated in replication fork restart by repriming (issue mark). Weve also observed that Pol downregulation in mammalian leads to deposition of DNA harm, thus uncovering a function for Pol during DNA replication in unperturbed cells and additional extending the function of the DNA polymerase outdoors TLS.4 Interestingly, in the ...
We are not concerned about mixed samples, because there is no preferential amplification with REPLI-g WGA. A small level of contamination from a secondary source cannot become a major component of the amplified material ...
Project Title: Development of high throughput assay for screening of novel DNA replication inhibitors for therapeutic purposes Supervisors: Professor Christian Speck, Professor David Rueda Funding: Tuition fees plus £21,000 pa stipend for 3.5 years Date posted: 09 July 2021 Closing date: 04 August 2021 The student will develop a fluorescence-based assay to identify novel DNA replication inhibitors for anti-cancer therapy. Inhibitors will be consequently characterised for their impact on the multi-step DNA replication process and on cancer cell growth. This interdisciplinary project will train the student in biochemistry, biophysics and drug screening. Project details , LMS 3.5-year Studentships , Apply ...
The global herpes simplex therapeutics market was worth approximately $2.9 billion in 2009. In 2001 the market was valued at approximately $1 billion and it grew at an approximate Compound Annual Growth Rate (CAGR) of 13.9% from 2001 to 2009. The global herpes simplex therapeutics market is expected to reach $7.3 billion by 2017, after growing at a CAGR of 12.3% from 2009. The primary factor contributing to the increase in revenues over the forecast period is the increase in disease incidence. Presently, the therapeutic regimen for herpes simplex is dominated by a range of DNA polymerase inhibitors which include Zovirax, Famvir, Valtrex, Denavir and OTC (Over-the-Counter) products such as Abreva and Aviralex. This market is a significantly genericized market, with many brands across the seven geographies covered in this report. Tablet prices range between $1 and $3 depending on the brand. The average annual cost of therapy for herpes simplex therapeutics ranges between $725 and $2,050 based on ...
Page 3: RPK Pharmaceuticals, Inc.: Valacyclovir hydrochloride is a nucleoside analogue DNA polymerase inhibitor indicated for: Adult Patients. (1.1) Cold Sores...
Pohjoismäki, J. L. O., Holmes, J. B., Wood, S. R., Yang, M. Y., Yasukawa, T., Reyes, A., Bailey, L. J., Cluett, T. J., Goffart, S., Willcox, S., Rigby, R. E., Jackson, A. P., Spelbrink, J. N., Griffith, J. D., Crouch, R. J., Jacobs, H. T., Holt, I. J., Mammalian mitochondrial DNA replication intermediates are essentially duplex but contain extensive tracts of RNA/DNA hybrid, J. Mol. Biol., 397, 1144-1155, 2010.04 ...
Fludarabine (NSC 118218) is a DNA synthesis inhibitor, which also inhibits phosphorylation of STAT1. Fludarabine, a pro-drug, is converted metabolically by dephosphorylation to the antimetabolite, F-ara-A. - Mechanism of Action & Protocol.
The MasterPure Complete DNA and RNA Purification Kit, MasterPure DNA Purification Kit, and MasterPure RNA Purification Kit use a novel, patent-pending technology that enables efficient purification of intact, high molecular weight DNA or RNA from a wide range of biological materials
Our working hypothesis focuses on the convergence of prosurvival, angiogenesis and motility signals at common pathways in the local tumor microenvironment for t...
Roylco. 15 real X-rays reveal a detailed look at common breaks. Use on a light table, hold up to a window, or place on white paper. Includes idea guide/fact sheet, which gives …
Human DNA polymerase alpha uses a combination of positive and negative selectivity to polymerize purine dNTPs with high fidelity Journal Article ...
There are no specific protocols for Recombinant Human DNA polymerase alpha protein (ab114839). Please download our general protocols booklet
DNA Polymerase Alpha 2 antibody LS-C169911 is an unconjugated rabbit polyclonal antibody to human DNA Polymerase Alpha 2 (POLA2) (aa55-104). Validated for WB.
TY - JOUR. T1 - Activation of deoxycytidine kinase by inhibition of DNA synthesis in human lymphocytes. AU - Csapó, Z.. AU - Sasvári, M.. AU - Spasokoukotskaja, T.. AU - Talianidis, Iannis. AU - Eriksson, Staffan. AU - Staub, M.. PY - 2001/1/15. Y1 - 2001/1/15. N2 - Deoxycytidine kinase (dCK, EC.2.7.1.74) is a key enzyme in the intracellular metabolism of 2-chlorodeoxyadenosine, 1β-D-arabinofuranosylcytosine, difluorodeoxycytidine, and other drugs used in chemotherapy of different leukaemias and solid tumours. Recently, stimulation of dCK activity was shown by these analogues and by other genotoxic agents such as etoposide and NaF, all of which cause severe inhibition of DNA synthesis in cell cultures. Here we describe that direct inhibition of DNA polymerases by aphidicolin stimulated dCK activity in normal lymphocytes and acute myeloid leukaemic cells, as well as in HL 60 promyelocytic cell cultures. Increased dCK activity was not due to new protein synthesis under our conditions, as ...
Analysis of Extracted DNA Samples Samples of DNA were quantitatively assayed using spectrophotometery by measuring absorbance at 260 nm (A260), 280 nm (A280), and 230 nm (A230) to determine concentration of ds DNA, and to measure A260 to A280 ratio (optimal ratio is equal to 1.8 or more and less than 2) and A260 to A230 ratio (optimal ratio is greater than 2.2). A280 is an estimate of concentration of non-DNA components. such as proteins, whereas A230 is measured to estimate concentration of other components such as detergents, peptides, carbohydrates, Inhibitors,research,lifescience,medical phenol and chloroform. Some randomly selected samples were assayed quantitatively by running on a 2% agarose gel electrophoresis, Inhibitors,research,lifescience,medical allowing the molecular weight of the DNA to be estimated. The electrophoresis resulted in a thick smear of DNA fragments with variable size ranging from very high molecular weight DNA to those about 100 bp in size (figure 1). Moreover, some ...
The Nucleon extraction chemistry rapidly and efficiently extracts high yields of pure, high molecular weight DNA. Nucleon is compatible for use with whole blood and other sample types such as tissue and saliva.. The DNA is not bound to a solid surface at any point in the process. This avoids exposing the DNA to shearing forces and promotes the recovery of high yields of highly intact DNA.. Performance data:. ...
Research Topics, Scientific Experts, Locale, Genomes and Genes, Publications, Species about Experts and Doctors on dna replication in Cincinnati, Ohio, United States
THERE GOES THE NEIGHBORHOODS Monday morning came around too soon. We both groaned at six when it was time to get up, eat at commons, and head out for our eight oclock class. No one ever adequately explains why all freshman year classes start so early. All the studies agree that teenagers are mindless zombies …
Have a look at common early signs of Parkinson disease that you need to look for. When dopamine levels in the brain decrease and when the neurons die, Parkinson
Covers following an eating plan for inflammatory bowel disease. Helps you learn more about how to eat so you can manage your symptoms but still get the nutrition you need. Looks at common problem foods.
Not all available origins of replication fire during a normal S-phase. But when replication is perturbed, otherwise dormant origins go to work, Woodward et al. show on page 673.. Cells initially respond to slowed replication by turning on the ATR-dependent checkpoint, which prevents other origins from firing and thus getting into trouble too. But if the cell decides it is time to recover from that checkpoint, the mechanism discovered by Woodward et al. may ensure that there are enough origins to get the job done.. The excess supply of origins arises from an excess of sites that have the minichromosome maintenance protein complexes, Mcm2-7. These complexes are loaded onto chromatin before S-phase and are required to license replication origins for use. However, the number of complexes loaded is much higher than the number normally used.. Working in Xenopus egg extract, Woodward et al. found that replication speed, origin spacing, and the slowing in response to the DNA polymerase inhibitor ...
Many anticancer agents induce DNA strand breaks or cause the accumulation of DNA replication intermediates. The protein encoded by ataxia-telangiectasia mutated and Rad 3-related (ATR) generates signals in response to these altered DNA structures and activates cellular survival responses. Accordingly, ATR has drawn increased attention as a potential target for novel therapeutic strategies designed to potentiate the effects of existing drugs. In this study, we use a unique panel of genetically modified human cancer cells to unambiguously test the roles of upstream and downstream components of the ATR pathway in the responses to common therapeutic agents. Upstream, the S-phase-specific cyclin-dependent kinase (Cdk) 2 was required for robust activation of ATR in response to diverse chemotherapeutic agents. While Cdk2-mediated ATR activation promoted cell survival after treatment with many drugs, signaling from ATR directly to the checkpoint kinase Chk1 was required for survival responses to only a ...
Regular annual exams and vaccines are some of the easiest things an animal owner can do to keep their pet healthy. We appreciate concerns related to over-vaccinating, the effects and the efficacy of vaccines. At Common Companion, we vaccinate your animal based on the latest medical evidence and vaccination guidelines. We provide only the vaccinations that are appropriate to your pets lifestyle. If you prefer to measure the protective antibodies of previous vaccines through blood work, we can also provide that service. At Common Companion, simply put, we provide your pet the same care that our personal pets receive and work hard to stay on top of the latest evidence-based medicine.. ...
In Eastwood and another v Magnox Electric plc; McCabe v Cornwall County Council and others, the House of Lords holds that, in cases where psychiatric injury is alleged to have been caused by acts of the employer committed prior to, and separately from the act of dismissal itself, a cause of action will exist at common law for damages.
Exception: System.Net.WebException: The operation has timed out at System.Net.HttpWebRequest.GetRequestStream(TransportContext& context) at System.Net.HttpWebRequest.GetRequestStream() at System.Web.Services.Protocols.SoapHttpClientProtocol.Invoke(String methodName, Object[] parameters) at localhost.ServeLink.GetLinks() in c:\Windows\Microsoft.NET\Framework64\v4.0.30319\Temporary ASP.NET Files\root\4b9dede0\cb220900\App_WebReferences.whmj_uhp.1.cs:line 263 at Common_RandomLink.LoadMe() in d:\websites\articlesite\Common\RandomLink.ascx.cs:line ...
It was surrounded by many plant species, so it was quite impossible to determine its host, and it is beyond recognition from the flowers. Based purely on the surrounding vegetation I will make a stab at Common Broomrape and will have to visit it again next summer when the flowers will be fresh. No Thyme was found nearby (and the habitat was wrong ...
Robert Ireland's synthesis of (±)-aphidicolin uses the Wolff rearrangement to do a tandem ring-contraction, and [2 + 2] ... aphidicolin and (.+-.)-.beta.-chamigrene". J. Org. Chem. 49 (6): 1001-1013. doi:10.1021/jo00180a010.CS1 maint: multiple names: ...
Aphidicolin is a mycotoxin originally known to be produced by the fungus, Cephalosporium aphidicola. This antiviral compound ... Starratt, A. N.; Loschiavo, S. R. (March 1974). "The production of aphidicolin by Nigrospora sphaerica". Canadian Journal of ...
McMurry, J. E.; Andrus A.; Ksander G. M.; Muesser, J. H.; Johnson, M. A. "Stereospecific Total Synthesis of Aphidicolin.". ...
Aphidicolin is an antibiotic isolated from the fungus Cephalosporum aphidicola. It is a reversible inhibitor of eukaryotic ... "Structural basis for inhibition of DNA replication by aphidicolin". Nucleic Acids Research. 42 (22): 14013-21. doi:10.1093/nar/ ...
Nagano, H; Ikegami, S (November 1980). "Aphidicolin: a specific inhibitor of eukaryotic DNA polymerase alpha". Seikagaku: The ...
Fragile site, aphidicolin type, common, fra(3)(p24.2) is a protein that in humans is encoded by the FRA3A gene. "Human PubMed ... "Entrez Gene: Fragile site, aphidicolin type, common, fra(3)(p24.2)". Retrieved 2014-08-05. CS1 maint: discouraged parameter ( ...
Sensitivity to novobiocin, bacitracin, anisomycin, aphidicolin, and rifampicin have been observed. However, no sensitivity has ...
"Isolation and Characterization of Aphidicolin and Chlamydosporol Derivatives from Tolypocladium inflatum". Journal of Natural ...
"Effects of monocerin on cell cycle progression in maize root meristems synchronized with aphidicolin". Plant Cell Reports. 15 ( ...
The chemical agent aphidicolin can be added to easily and effectively synchronize chloroplast division. The peroxisome division ... of chloroplast division in the ultramicro-alga Cyanidioschyzon merolae by treatment with both light and aphidicolin". J. Phycol ...
FRA3A encoding protein Fragile site, aphidicolin type, common, fra(3)(p24.2). *FRMD4B encoding protein FERM domain containing ...
"FRAXD fragile site, aphidicolin type, common, fra(X)(q27.2) D [Homo sapiens (human)] - Gene - NCBI". www.ncbi.nlm.nih.gov. " ... FRAXD or FRAXD gene is a gene symbol for fragile site, aphidicolin type, common, fra(X)(q27.2) D. The locus of the gene is ...
3-deoxyaphidicolin and aphidicolin-17-monoacetate". Nucleic Acids Research. 11 (4): 1197-209. doi:10.1093/nar/11.4.1197. PMC ...
3-deoxyaphidicolin and aphidicolin-17-monoacetate". Nucleic Acids Res. 11: 1197-2000. doi:10.1093/nar/11.4.1197. PMC 325786. v ...
Glover, TW; Berger, C; Coyle, J; Echo, B (1984). "DNA polymerase alpha inhibition by aphidicolin induces gaps and breaks at ...
... a key enzyme responsible for formation of an unusual diterpene skeleton in biosynthesis of aphidicolin". Journal of the ... "Cloning of a gene cluster responsible for the biosynthesis of diterpene aphidicolin, a specific inhibitor of DNA polymerase ...
In the presence of DNA damage or replication stress (UV light, methyl methanesulfonate, hydroxyurea or aphidicolin), the POLD4/ ...
Further studies to support the theory of cell-proliferation were done by introducing then removing the drug aphidicolin which ...
Subsequent discoveries included alamethicin, aphidicolin, brefeldin A, cephalosporin, cerulenin, citromycin, eupenifeldin, ...
... or aphidicolin, each of which had been documented to exert its effects in a particular phase of the cell cycle. Surprisingly, ...
He and his students published syntheses of 2-deoxyribose, aaptamine, aphidicolin, apiose, arachidonic acid, arcyriaflavin B, ...
... aphidicolin type, common, fra(3)(p24.2) FRMD4B encoding protein FERM domain containing 4B GMPPB: GDP-mannose pyrophosphorylase ...
Aphidicolin Experimental drugs and drug precursors: Parthenolide, Puromycin, Rapamycin, Anisomycin, Thapsigargin, cyclopamine, ...
Most notable are prostaglandin F2α, narwedine, aphidicolin, taxusin, Taxol and hemibrevetoxin B. Dr. Holton also serves as ...
ISBN 978-0-12-465327-6. DeFilippes, FM (Nov 1984). "Effect of aphidicolin on vaccinia virus: isolation of an aphidicolin- ... Aphidicolin is a reversible inhibitor of eukaryotic nuclear DNA replication. It blocks the cell cycle at early S phase. It is a ... Natural aphidicolin is a secondary metabolite of the fungus Nigrospora oryzae. Dhillon VS, Husain SA, Ray GN (2003). " ... Aphidicolin product page Archived June 26, 2006, at the Wayback Machine from Fermentek v t e. ...
7-Dehydrocholesterol is the precursor of cholecalciferol.[8] Within the epidermal layer of skin, 7-dehydrocholesterol undergoes an electrocyclic reaction as a result of UVB light at wavelengths between 290 and 315 nm, with peak synthesis occurring between 295 and 300 nm.[32] This results in the opening of the vitamin precursor B-ring through a conrotatory pathway making previtamin D3 (pre-cholecalciferol).[33] In a process which is independent of UV light, the pre-cholecalciferol then undergoes a [1,7] antarafacial sigmatropic rearrangement [34] and therein finally isomerizes to form vitamin D3. The active UVB wavelengths are present in sunlight, and sufficient amounts of cholecalciferol can be produced with moderate exposure of the skin, depending on the strength of the sun.[32] Time of day, season, and altitude affect the strength of the sun, and pollution, cloud cover or glass all reduce the amount of UVB exposure. Exposure of face, arms and legs, averaging 5-30 minutes twice per week, may be ...
Because of the structural diversity of alkaloids, there is no single method of their extraction from natural raw materials.[178] Most methods exploit the property of most alkaloids to be soluble in organic solvents [7] but not in water, and the opposite tendency of their salts. Most plants contain several alkaloids. Their mixture is extracted first and then individual alkaloids are separated.[179] Plants are thoroughly ground before extraction.[178][180] Most alkaloids are present in the raw plants in the form of salts of organic acids.[178] The extracted alkaloids may remain salts or change into bases.[179] Base extraction is achieved by processing the raw material with alkaline solutions and extracting the alkaloid bases with organic solvents, such as 1,2-dichloroethane, chloroform, diethyl ether or benzene. Then, the impurities are dissolved by weak acids; this converts alkaloid bases into salts that are washed away with water. If necessary, an aqueous solution of alkaloid salts is again made ...
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... is common as a dietary supplement and as a fragrance ingredient for cosmetics products.[1] As the main fragrance of citrus peels, D-limonene is used in food manufacturing and some medicines, such as a flavoring to mask the bitter taste of alkaloids, and as a fragrance in perfumery, aftershave lotions, bath products, and other personal care products.[1] D-Limonene is also used as a botanical insecticide.[1][14] D-Limonene is used in the organic herbicide "Avenger".[15] It is added to cleaning products, such as hand cleansers to give a lemon or orange fragrance (see orange oil) and for its ability to dissolve oils.[1] In contrast, L-limonene has a piny, turpentine-like odor. Limonene is used as a solvent for cleaning purposes, such as adhesive remover, or the removal of oil from machine parts, as it is produced from a renewable source (citrus essential oil, as a byproduct of orange juice manufacture).[11] It is used as a paint stripper and is also useful as a fragrant alternative to ...
Many molecules that are considered to be "dietary fiber" are so because humans lack the necessary enzymes to split the glycosidic bond and they reach the large intestine. Many foods contain varying types of dietary fibers, all of which contribute to health in different ways. Dietary fibers make three primary contributions: bulking, viscosity and fermentation.[49] Different fibers have different effects, suggesting that a variety of dietary fibers contribute to overall health. Some fibers contribute through one primary mechanism. For instance, cellulose and wheat bran provide excellent bulking effects, but are minimally fermented. Alternatively, many dietary fibers can contribute to health through more than one of these mechanisms. For instance, psyllium provides bulking as well as viscosity. Bulking fibers can be soluble (i.e., psyllium) or insoluble (i.e., cellulose and hemicellulose). They absorb water and can significantly increase stool weight and regularity. Most bulking fibers are not ...
ISBN 978-0-12-465327-6. DeFilippes, FM (Nov 1984). "Effect of aphidicolin on vaccinia virus: isolation of an aphidicolin- ... Aphidicolin is a reversible inhibitor of eukaryotic nuclear DNA replication. It blocks the cell cycle at early S phase. It is a ... Natural aphidicolin is a secondary metabolite of the fungus Nigrospora oryzae. Dhillon VS, Husain SA, Ray GN (2003). " ... Aphidicolin product page Archived June 26, 2006, at the Wayback Machine from Fermentek v t e. ...
G2 and G1 phases after Aphidicolin synchronization. For this purpose we measured diffusion rates of EGFP molecules using ... Aphidicolin synchronization influences cell size. HeLa cells were synchronized by incubation with aphidicolin (5 µg/ml) for 24 ... Aphidicolin synchronization. Ready-made aphidicolin solution (1 mg/ml) was purchased from Sigma-Aldrich (USA). Cells were ... Aphidicolin efficiently synchronizes HeLa cells. First, we tested if addition of aphidicolin indeed would allow robust ...
Shop a large selection of Signal Transduction Reagents and Kits products and learn more about Alfa Aesar Aphidicolin Quantity: ...
Aphidicolin, DNA polymerase alpha, delta and epsilon inhibitor (CAS 38966-21-1), with ,98% purity. Join researchers using our ...
Aphidicolin, CAS: 38966-21-1, is a novel tetracyclic diterpene antibiotic and also a DNA synthesis inhibitor in eukaryotes.. ... Aphidicolin is useful for cell synchronization. Aphidicolin blocks the cell cycle at early S phase. Aphidicolin prolongs the ... Aphidicolin (CAS 38966-21-1) Aphidicolin , CAS 38966-21-1 is rated 5.0 out of 5 by 3. ... Aphidicolin (CAS 38966-21-1) Product Citations See how others have used Aphidicolin (CAS 38966-21-1). Click on the entry to ...
Inhibition of DNA repair with aphidicolin enhances sensitivity of targets to tumor necrosis factor.. J F Gera, C Fady, A ... Aphidicolin inhibited repair and consistently sensitized to TNF cytotoxicity, decreasing the ID50 for TNF at least 10- to 50- ... In targets sensitized with aphidicolin, TNF-induced strand breakage was accelerated, being detected by 4 h of culture in the ... To inhibit repair, nontoxic concentrations of aphidicolin (inhibitor of DNA polymerase-alpha) and dideoxythymidine (inhibitor ...
C growth medium and 6 μM aphidicolin (catalog no. 10797; Fluka). The nocodazole and aphidicolin were both dissolved in dimethyl ... Because exposure to aphidicolin causes cell cycle arrest in the G1/S stage for both T. brucei and Giardia, it can be assumed ... A and B) Subjecting a control culture with a 5:1 ratio of G2 to G1 cells (A) to treatment with 6 μM aphidicolin (Aph) for 6 h ... Arrest of Giardia cell cycle with nocodazole and aphidicolin. (A) Starting culture with a 5:1 ratio of G2 to G1 cells. (B) ...
2705 (1973). [3] Aphidicolin: (a) E. J . Corey, M.A . Tius, J. Das. J. Am. Chem. SOC.102. 1744 (1980); J. E. McMurry, A . ... An Expeditious and Efficient Entry into the Aphidicolin and Related Natural Products Ring Skeleton.. код для вставки. код для ... The strategy for this new entry into the aphidicolin (1) and related structures depends on two key operations; firstly a ... An Expeditious and Efficient Entry into the Aphidicolin and Related Natural Products Ring Skeleton**] By K . C. Nicolaou and ...
Purification of the cdc8 protein of Saccharomyces cerevisiae by complementation in an aphidicolin-sensitive in vitro DNA ... Purification of the cdc8 protein of Saccharomyces cerevisiae by complementation in an aphidicolin-sensitive in vitro DNA ...
... sterile-filtered solution of Aphidicolin in DMSO. - Find MSDS or SDS, a COA, data sheets and more information. ... Aphidicolin - CAS 38966-21-1 - Calbiochem CAS 38966-21-1 A 30 mM (1 mg/98 µL) ... InSolution™ Aphidicolin - CAS 38966-21-1 - Calbiochem. 504744 Sigma-AldrichInSolution™ Aphidicolin - CAS 38966-21-1 - ... More,, A 30 mM (1 mg/98 µL) sterile-filtered solution of Aphidicolin in DMSO. Less,, MSDS (material safety data sheet) or SDS, ...
Aphidicolin is an antiviral antibiotic produced by Nigrospora oryzae and other fungi. Aphidicolin inhibits the growth of ... Aphidicolin may possibly be used for controlling excessive cell proliferation in cancer, psoriasis or other dermatitis with ... Aphidicolin inhibits the growth of eukaryotic cells and certain animal viruses by selectively inhibiting the cellular ... psoriasis or other dermatitis with little or no adverse effect upon non-multiplying cells.Aphidicolin is a tool in cell ...
Aphidicolin (CAS 38966-21-1) specifically inhibits DNA polymerase α & δ, arresting the cell cycle at early S phase while ... Aphidicolin , DNA polymerase inhibitor Aphidicolin (38966-21-1) specifically inhibits DNA polymerase α and δ via binding to the ... Aphidicolin potentiates apoptosis induction induced by other agents3. Increases gene amplification frequency in HeLa S3 cells ... 1993), Aphidicolin potentiates apoptosis induced by arabinosyl nucleosides in human myeloid leukemia cell lines; Biochem. ...
HomeThe G1/S inhibitor aphidicolin neither affec. The G1/S inhibitor aphidicolin neither affec. By admin June 11, 2019 ...
Aphidicolin-resistant recombinant progeny were formed with recombination frequencies (0.4 to 2.6%) compatible with intragenic ... A wide range of stable aphidicolin sensitivities was represented amongst a collection of virus strains with no prior exposure ... Of 16 unrelated PAA-resistant variants, 7 were hypersensitive to aphidicolin. A number of mutants with temperature-sensitive ( ... ts) lesions in the polymerase gene also showed increased aphidicolin sensitivity (e.g. HSV-1[mP17]tsH) or aphidicolin ...
Aphidicolin DNA polymerase α, δ and ε inhibitor 5736. Show Size & Price ...
Aphidicolin treatments. Embryos were incubated for 2 hours (from 70% epiboly to the 3-somite stage) [compared to an estimated 4 ... Marheineke, K. and Hyrien, O. (2001). Aphidicolin triggers a block to replication origin firing in Xenopus egg extracts. J. ... To block cell proliferation, we incubated wild-type embryos in aphidicolin from the onset of her5 expression until early ... in embryo medium containing 1 or 10 μg/ml aphidicolin (Sigma A-9914) at 28.5°C (Marheineke and Hyrien, 2001). The embryos were ...
Robert Irelands synthesis of (±)-aphidicolin uses the Wolff rearrangement to do a tandem ring-contraction, and [2 + 2] ... aphidicolin and (.+-.)-.beta.-chamigrene". J. Org. Chem. 49 (6): 1001. doi:10.1021/jo00180a010. CS1 maint: Multiple names: ...
Aphidicolin promotes repair of potentially lethal damage in irradiated mammalian cell synchronized in s-phase. ... Iliakis, G and Nusse, M, "Aphidicolin promotes repair of potentially lethal damage in irradiated mammalian cell synchronized in ...
Fuster C, Miró R, Egozcue J, Rigola MA, Ribas M. Combined effects of cyclosporine A and Aphidicolin on human lymphocytes. ... Combined effects of cyclosporine A and Aphidicolin on human lymphocytes. C. Fuster, R. Miró, J. Egozcue, M.A. Rigola, M. Ribas ... Fuster, C. ; Miró, R. ; Egozcue, J. ; Rigola, M.A. ; Ribas, M. / Combined effects of cyclosporine A and Aphidicolin on human ... Combined effects of cyclosporine A and Aphidicolin on human lymphocytes. / Fuster, C.; Miró, R.; Egozcue, J.; Rigola, M.A.; ...
Aphidicolin (APH), a tetracyclic diterpene, exhibits specific cytotoxic action against NB cells. The purpose of this study was ... Treatment of drug-resistant human neuroblastoma cells with cyclodextrin inclusion complexes of aphidicolin. ... Treatment of drug-resistant human neuroblastoma cells with cyclodextrin inclusion complexes of aphidicolin. Anti-cancer drugs, ...
Hydroxyurea and aphidicolin (HUA) treatment. HUA treatment was performed as described previously (Harris and Hartenstein, 1991 ... hydroxyurea and aphidicolin (HUA; supplementary material Fig. S2A-E) (Harris and Hartenstein, 1991). When soaked in HUA ... 150 mM aphidicolin (A0781; Sigma) and 0.1× MMR] until the desired stages. ...
Aphidicolin Type, Common, Fra(6)(Q15), including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards ... FRA6G (Fragile Site, Aphidicolin Type, Common, Fra(6)(Q15)) is an Uncategorized gene. ...
Aphidicolin. Miscellaneous Diterpenes. Taxol. COMPARATIVE DESIGN: CLASSICS IN ALKALOID SYNTHESIS. Introduction. Lycopodium ...
... and then treated with aphidicolin for 3 h before nucleofection. The duration of aphidicolin treatment was shortened, because a ... Aphidicolin was the only tested treatment that enhanced NHEJ and HDR. The NHEJ frequency increased from 8% to 18% at 30 ρmol ... Aphidicolin and nocodazole increased the NHEJ frequency slightly at 30 ρmol of Cas9; at 100 ρmol of Cas9, the detection limit ... Aphidicolin was added into the growth media immediately after nucleofection to 2 μg/ml. The mean % HDR and standard deviation ( ...
... aphidicolin) and interference with enzymes dealing with topological DNA stress (camptothecin, etoposide). As a variety of ... Keywords: replication stress; cisplatin; aphidicolin; hydroxyurea; camptothecin; etoposide; cancer replication stress; ... aphidicolin) and interference with enzymes dealing with topological DNA stress (camptothecin, etoposide). As a variety of ...
Differential effects of aphidicolin on replicative dna synthesis and unscheduled dna synthesis in permeable mouse sarcoma cells ... Seki, S; Oda, T; and Ohashi, M, "Differential effects of aphidicolin on replicative dna synthesis and unscheduled dna synthesis ...
DNA POLYMERASE-EPSILON - APHIDICOLIN INHIBITION AND THE RELATIONSHIP BETWEEN POLYMERASE AND EXONUCLEASE ACTIVITY Journal ...
Aphidicolin Type, Common, Fra(X)(P22.31) B, including: function, proteins, disorders, pathways, orthologs, and expression. ... FRAXB (Fragile Site, Aphidicolin Type, Common, Fra(X)(P22.31) B) is an Uncategorized gene. ...
DNA polymerase inhibitor aphidicolin; RNA polymerase inhibitor actinomycin D; protein synthesis inhibitor cycloheximide; ...
... aphidicolin glycinate; apoptosis gene modulators; apoptosis regulators; apurinic acid; ara-CDP-DL-PTBA; arginine deaminase; ...
  • These compounds act on replication by direct interaction with DNA causing DNA crosslinks and bulky lesions (cisplatin), chemical interference with the metabolism of deoxyribonucleotide triphosphates (hydroxyurea), direct inhibition of the activity of replicative DNA polymerases (aphidicolin) and interference with enzymes dealing with topological DNA stress (camptothecin, etoposide). (mdpi.com)
  • A second frequent strategy for replication interference in cancer chemotherapy makes use of antimetabolites to block nucleotide biosynthesis or DNA polymerization, as for the ribonucleotide reductase inhibitor hydroxyurea (HU) or the DNA polymerase inhibitor aphidicolin (APH). (rupress.org)
  • atr mutants were hypersensitive to hydroxyurea (HU), aphidicolin, and UV-B light but only mildly sensitive to γ-radiation. (plantcell.org)
  • Aphidicolin is a tetracyclic diterpene antibiotic isolated from the fungus, Cephalosporum aphidicola with antiviral and antimitotic properties. (wikipedia.org)
  • In addition, Aphidicolin is a tetracyclic diterpene antibiotic with antiviral and antimitotic properties. (scbt.com)
  • An Expeditious and Efficient Entry into the Aphidicolin and Related Natural Products Ring Skeleton'**] By K . C. Nicolaou and Robert E. Zipkid' The potent antiviral and antimitotic properties of aphidicolin ( 1 ) [ l J ,coupled with its novel and highly unusual molecular framework present a formidable and important challenge. (docme.ru)
  • Aphidicolin is an antiviral antibiotic produced by Nigrospora oryzae and other fungi. (fermentek.com)
  • The nature of residues determined at any one of a constellation of separate sites within the polymerase locus can determine resistance or sensitivity to antiviral drugs and aphidicolin hypersensitivity associated with changes at the polymerase locus facilitates high resolution genetic analysis of this locus. (microbiologyresearch.org)
  • Aphidicolin has been reported as an antiviral, antitumoral and leishmanicidal hit. (usp.br)
  • To inhibit repair, nontoxic concentrations of aphidicolin (inhibitor of DNA polymerase-alpha) and dideoxythymidine (inhibitor of DNA polymerase-beta and gamma) were used. (jimmunol.org)
  • Aphidicolin is a novel tetracyclic diterpene antibiotic and also a DNA synthesis inhibitor in eukaryotes. (scbt.com)
  • Aphidicolin is a diterpene fungal metabolite that blocks the cell cycle at early S-phase. (scbt.com)
  • Aphidicolin (APH), a tetracyclic diterpene, exhibits specific cytotoxic action against NB cells. (kent.ac.uk)
  • Aphidicolin is a tetracyclic diterpene antibiotic which kills human neuroblastoma cells (NB) in vitro while it has no significant effect on the viability of different human cell types including normal embryonal cells. (sav.sk)
  • In this work endophytic and soil fungal strains were applied for the biotransformation of the bioactive terpenes enhydrin (sesquiterpene lactone) and aphidicolin (diterpene). (usp.br)
  • Deoxycytidine, which competitively inhibits binding of aphidicolin to DNA polymerase, blocked the sensitization in a concentration-dependent fashion. (jimmunol.org)
  • Aphidicolin inhibits the growth of eukaryotic cells and certain animal viruses by selectively inhibiting the cellular replication of DNA polymerase II or the viral-induced DNA polymerases. (fermentek.com)
  • Aphidicolin (38966-21-1) specifically inhibits DNA polymerase α and δ via binding to the enzyme, in eukaryotic cells such as the HeLa cell line, without affecting other DNA polymerases 1 . (focusbiomolecules.com)
  • Natural aphidicolin is a secondary metabolite of the fungus Nigrospora oryzae. (wikipedia.org)
  • Aphidicolin is a reversible inhibitor of eukaryotic nuclear DNA replication. (wikipedia.org)
  • It is expected, that aphidicolin inhibition does not affect DNA repair nor biosynthesis of RNA, and synchronized cells remain in physiological state and restart DNA replication shortly after removal of the compound 19 . (nature.com)
  • Aphidicolin was also effective in inhibiting megakaryocytic differentiation of other leukemia cell lines such as human erythroleukemia (HEL) and K562 cell lines induced with TPA, suggesting the close interplay of DNA replication and phenotypic expression in megakaryopoiesis. (fujita-hu.ac.jp)
  • Implication of RPA32 phosphorylation in S-phase checkpoint signalling at replication forks stalled with aphidicolin in Xenopus egg extracts. (cngb.org)
  • Aphidicolin: a specific inhibitor of nuclear DNA replication in eukaryotes: S. Spadari, et al. (nordicbiosite.com)
  • Structural basis for inhibition of DNA replication by aphidicolin: A.G. Baranovskiy, et al. (nordicbiosite.com)
  • Inhibition of DNA repair with aphidicolin enhances sensitivity of targets to tumor necrosis factor. (jimmunol.org)
  • G 2 arrest using nocodazole was combined with G 1 arrest using aphidicolin, a drug that presumably acts through the inhibition of polymerase-dependent DNA synthesis ( 8 , 12 , 14 , 25 ). (asm.org)
  • Differential effects of aphidicolin on replicative dna synthesis and u" by S Seki, T Oda et al. (jax.org)
  • Differential effects of aphidicolin on replicative dna synthesis and unscheduled dna synthesis in permeable mouse sarcoma cells. (jax.org)
  • and Ohashi, M, "Differential effects of aphidicolin on replicative dna synthesis and unscheduled dna synthesis in permeable mouse sarcoma cells. (jax.org)
  • All the above mentioned processes of DNA synthesis are reversibly inhibited by aphidicolin (C50 from 3-13 microM). (nih.gov)
  • Obtainment of aphidicolin and enhydrin derivatives through biotransformation and semi-synthesis and evaluation of leishmanical activity. (usp.br)
  • To make these extracts, we prepared a cycling Xenopus egg extract ( 16 , 17 ) and then added aphidicolin, an inhibitor of α-DNA polymerase ( 18 ), and demembranated Xenopus sperm nuclei ( 19 ). (sciencemag.org)
  • The biosynthesis of aphidicolin. (bl.uk)
  • Treatment of drug-resistant human neuroblastoma cells with cyclodextrin inclusion complexes of aphidicolin. (kent.ac.uk)
  • Cytotoxicity of aphidicolin and its derivatives against neuroblastoma cells in vitro: synergism with doxorubicin and vincristine: M. Michaelis, et al. (nordicbiosite.com)
  • Aphidicolin was first isolated from Cephelosporium aphidicola. (scbt.com)
  • The research presented here demonstrates reversible cell cycle arrest in G 1 /S and G 2 /M with aphidicolin and nocodazole, respectively. (asm.org)
  • Aphidicolin prolongs the half life of DNA methyltransferase. (scbt.com)
  • Effect of aphidicolin on DNA methyltransferase in the nucleus: I. Suetake, et al. (nordicbiosite.com)
  • Ribas, M. / Combined effects of cyclosporine A and Aphidicolin on human lymphocytes . (uab.cat)
  • In this paper we address this topic by constant observation of nanoviscosity of HeLa cells cytoplasm during S, G2 and G1 phases after Aphidicolin synchronization. (nature.com)
  • Of 16 unrelated PAA-resistant variants, 7 were hypersensitive to aphidicolin. (microbiologyresearch.org)
  • Moreover, the selection of aphidicolin-resistant mutants from hypersensitive variants with independent PAA resistance or ts mutations in the polymerase gene could result in co-selection for PAA-sensitive and ts + phenotypes. (microbiologyresearch.org)
  • Confirmation that multiple independent mutations could determine aphidicolin hypersensitivity was obtained by studies of recombination between independent hypersensitive variants. (microbiologyresearch.org)
  • Aphidicolin inhibited repair and consistently sensitized to TNF cytotoxicity, decreasing the ID50 for TNF at least 10- to 50-fold. (jimmunol.org)
  • Despite not sharing structural homology with dCTP (deoxycytidine triphosphate) aphidicolin binds at this nucleotide-binding site 18 . (nature.com)
  • Aphidicolin and bleomycin induced chromosome damage as biomarker of mutagen sensitivity: a twin study: B. Tedeschi, et al. (nordicbiosite.com)
  • HDHB-depleted cells showed more aphidicolin-induced chromosome breaks than control-depleted cells. (vanderbilt.edu)
  • One of inhibitors of the cell cycle - used for the purpose of synchronization - is aphidicolin. (nature.com)
  • Aphidicolin is useful for cell synchronization. (scbt.com)
  • In medicine, Aphidicolin may be useful for controlling excessive cell proliferation in patients with cancer, psoriasis or other dermatitis with little or no adverse effect upon non-multiplying cells. (scbt.com)
  • Aphidicolin is a specific inhibitor of DNA polymerase α and δ in eukaryotic cells and in some viruses of animal origin. (scbt.com)
  • Aphidicolin, a specific inhibitor of DNA polymerase α, completely inhibited the differentiation induction of MEG-O1 cells with TPA measured by either GP IIb/IIIa expression or multinuclear cell formation. (fujita-hu.ac.jp)
  • Aphidicolin-induced nuclear elongation in tobacco BY-2 cells: H. Yashura, et al. (nordicbiosite.com)
  • In the present study, we tested whether aphidicolin encapsulated in liposomes kills NB cells with the efficacy superior to that of unencapsulated aphidicolin. (sav.sk)
  • The treatment with encapsulated aphidicolin at a concentration of 200 nmol for 5 days killed all cells of three human NB cell lines. (sav.sk)
  • In contrast, at least 30% of the cells survived 5 days of treatment with 200 nmol unencapsulated aphidicolin. (sav.sk)
  • The results showed that aphidicolin killing of human NB cells may be increased by encapsulation in liposomes. (sav.sk)
  • A number of mutants with temperature-sensitive ( ts ) lesions in the polymerase gene also showed increased aphidicolin sensitivity (e.g. (microbiologyresearch.org)
  • G2 arrest also was observed in wild-type plants in response to aphidicolin but was defective in atr mutants, resulting in compaction of nuclei and subsequent cell death. (plantcell.org)
  • Aphidicolin is soluble in DMSO (50 mg/ml) and Methanol (10 mg/ml) purged in inert gas. (scbt.com)
  • A 30 mM (1 mg/98 µL) sterile-filtered solution of Aphidicolin in DMSO. (emdmillipore.com)
  • in contrast, H1 activity in aphidicolin-treated extracts remained at a constant, low amount for 6 hours ( 20 , 21 ). (sciencemag.org)
  • Time-lapse videomicroscopy of aphidicolin-treated extracts revealed that nuclear envelope breakdown did not occur during the 6-hour experiment ( 20 ). (sciencemag.org)
  • We characterized the pattern of centrosome reproduction in aphidicolin-treated extracts with time-lapse videomicroscopy. (sciencemag.org)
  • A wide range of stable aphidicolin sensitivities was represented amongst a collection of virus strains with no prior exposure to this drug, but viruses with polymerase mutations selected for resistance to phosphonoacetic acid (PAA) or to acycloguanosine typically showed increased sensitivity to aphidicolin. (microbiologyresearch.org)
  • Antileishmanial activities of aphidicolin and its semisynthetic derivatives: O. Kayser, et al. (nordicbiosite.com)
  • The biotransformation experiments using aphidicolin as substrate were not successful in the production of derivatives. (usp.br)
  • The derivatives of aphidicolin were more efficiently prepared through semi-synthetic approaches. (usp.br)
  • Five aphidicolin semi-synthetic derivatives were obtained: two silicon mono-silyl ether derivatives, one silicon bis-silyl ether derivative, one tetra-acetylated derivative and one triacetylated derivative. (usp.br)
  • The segregation of other markers ( ts , plaque morphology) amongst recombinant progeny permitted the orientation of multiple determinants of PAA resistance and aphidicolin hypersensitivity with respect to other markers in the polymerase gene and in other genes. (microbiologyresearch.org)
  • Aphidicolin prevents mitotic cell division by interfering with the activity of DNA polymerase-alpha: S. Ikegami, et al. (nordicbiosite.com)
  • Human papillomavirus episome stability is reduced by aphidicolin and controlled by DNA damage response pathways: T. G. Edwards, et al. (nordicbiosite.com)
  • Unexpectedly, aphidicolin decreased the distance SC migrated on glass, but had no effect on SC migration on nanofibers. (minervamedica.it)
  • Aphidicolin promotes repair of potentially lethal damage in irradiated mammalian cell synchronized in s-phase. (jax.org)