A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
A subclass of dual specificity phosphatases that play a role in the progression of the CELL CYCLE. They dephosphorylate and activate CYCLIN-DEPENDENT KINASES.
Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.
Proteins that control the CELL DIVISION CYCLE. This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS.
Phosphoprotein with protein kinase activity that functions in the G2/M phase transition of the CELL CYCLE. It is the catalytic subunit of the MATURATION-PROMOTING FACTOR and complexes with both CYCLIN A and CYCLIN B in mammalian cells. The maximal activity of cyclin-dependent kinase 1 is achieved when it is fully dephosphorylated.
Together with the Apc2 subunit, forms the catalytic core of the E3 ubiquitin ligase, anaphase-promoting complex-cyclosome. It has a RING H2 domain which interacts with the cullin domain of Apc2. Apc11 also interacts with the E2 ubiquitin ligases involved in APC-C ubiquitination reactions.
A subunit of the anaphase-promoting complex whose primary function is to provide structural support for the catalytic and substrate-recognition modules of the complex. Apc5, along with Apc4, tethers the tetratricopeptide-coactivator binding subcomplex to the main structural subunit, Apc1.
A highly conserved subunit of the anaphase-promoting complex (APC-C) containing multiple 34 amino acid tetratricopeptide repeats. These domains, also found in Apc3, Apc6, and Apc8, have been shown to mediate protein-protein interactions, suggesting that Apc7 may assist in coordinating the juxtaposition of the catalytic and substrate recognition module subunits relative to coactivators and APC-C inhibitors.
The largest subunit of the anaphase-promoting complex. It acts primarily as a scaffold for the proper organization and arrangement of subunits. The C-terminal region of Apc1 contains a series of tandem amino acid repeats that are also seen in the 26S proteasome regulatory particle, and may assist with forming and stabilizing protein-protein interactions.
A negative regulator of beta-catenin signaling which is mutant in ADENOMATOUS POLYPOSIS COLI and GARDNER SYNDROME.
Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.
Databases devoted to knowledge about specific genes and gene products.
Variant forms of the same gene, occupying the same locus on homologous CHROMOSOMES, and governing the variants in production of the same gene product.
Any method used for determining the location of and relative distances between genes on a chromosome.
Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.
A species of imperfect fungi from which the antibiotic nidulin is obtained. Its teleomorph is Emericella nidulans.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
The process by which the CYTOPLASM of a cell is divided.
In a prokaryotic cell or in the nucleus of a eukaryotic cell, a structure consisting of or containing DNA which carries the genetic information essential to the cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
An E3 ubiquitin ligase primarily involved in regulation of the metaphase-to-anaphase transition during MITOSIS through ubiquitination of specific CELL CYCLE PROTEINS. Enzyme activity is tightly regulated through subunits and cofactors, which modulate activation, inhibition, and substrate specificity. The anaphase-promoting complex, or APC-C, is also involved in tissue differentiation in the PLACENTA, CRYSTALLINE LENS, and SKELETAL MUSCLE, and in regulation of postmitotic NEURONAL PLASTICITY and excitability.
Complexes of enzymes that catalyze the covalent attachment of UBIQUITIN to other proteins by forming a peptide bond between the C-terminal GLYCINE of UBIQUITIN and the alpha-amino groups of LYSINE residues in the protein. The complexes play an important role in mediating the selective-degradation of short-lived and abnormal proteins. The complex of enzymes can be broken down into three components that involve activation of ubiquitin (UBIQUITIN-ACTIVATING ENZYMES), conjugation of ubiquitin to the ligase complex (UBIQUITIN-CONJUGATING ENZYMES), and ligation of ubiquitin to the substrate protein (UBIQUITIN-PROTEIN LIGASES).
Highly conserved proteins that specifically bind to and activate the anaphase-promoting complex-cyclosome, promoting ubiquitination and proteolysis of cell-cycle-regulatory proteins. Cdc20 is essential for anaphase-promoting complex activity, initiation of anaphase, and cyclin proteolysis during mitosis.
The phase of cell nucleus division following PROPHASE, when the breakdown of the NUCLEAR ENVELOPE occurs and the MITOTIC SPINDLE APPARATUS enters the nuclear region and attaches to the KINETOCHORES.
A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species.
A microtubule structure that forms during CELL DIVISION. It consists of two SPINDLE POLES, and sets of MICROTUBULES that may include the astral microtubules, the polar microtubules, and the kinetochore microtubules.
Securin is involved in the control of the metaphase-anaphase transition during MITOSIS. It promotes the onset of anaphase by blocking SEPARASE function and preventing proteolysis of cohesin and separation of sister CHROMATIDS. Overexpression of securin is associated with NEOPLASTIC CELL TRANSFORMATION and tumor formation.

The schizosaccharomyces pombe dim1(+) gene interacts with the anaphase-promoting complex or cyclosome (APC/C) component lid1(+) and is required for APC/C function. (1/25)

The Schizosaccharomyces pombe dim1(+) gene is required for entry into mitosis and for chromosome segregation during mitosis. To further understand dim1p function, we undertook a synthetic lethal screen with the temperature-sensitive dim1-35 mutant and isolated lid (for lethal in dim1-35) mutants. Here, we describe the temperature-sensitive lid1-6 mutant. At the restrictive temperature of 36 degrees C, lid1-6 mutant cells arrest with a "cut" phenotype similar to that of cut4 and cut9 mutants. An epitope-tagged version of lid1p is a component of a multiprotein approximately 20S complex; the presence of lid1p in this complex depends upon functional cut9(+). lid1p-myc coimmunoprecipitates with several other proteins, including cut9p and nuc2p, and the presence of cut9p in a 20S complex depends upon the activity of lid1(+). Further, lid1(+) function is required for the multiubiquitination of cut2p, an anaphase-promoting complex or cyclosome (APC/C) target. Thus, lid1p is a component of the S. pombe APC/C. In dim1 mutants, the abundances of lid1p and the APC/C complex decline significantly, and the ubiquitination of an APC/C target is abolished. These data suggest that at least one role of dim1p is to maintain or establish the steady-state level of the APC/C.  (+info)

Cell cycle control of Cdc7p kinase activity through regulation of Dbf4p stability. (2/25)

In Saccharomyces cerevisiae, the heteromeric kinase complex Cdc7p-Dbf4p plays a pivotal role at replication origins in triggering the initiation of DNA replication during the S phase. We have assayed the kinase activity of endogenous levels of Cdc7p kinase by using a likely physiological target, Mcm2p, as a substrate. Using this assay, we have confirmed that Cdc7p kinase activity fluctuates during the cell cycle; it is low in the G1 phase, rises as cells enter the S phase, and remains high until cells complete mitosis. These changes in kinase activity cannot be accounted for by changes in the levels of the catalytic subunit Cdc7p, as these levels are constant during the cell cycle. However, the fluctuations in kinase activity do correlate with levels of the regulatory subunit Dbf4p. The regulation of Dbf4p levels can be attributed in part to increased degradation of the protein in G1 cells. This G1-phase instability is cdc16 dependent, suggesting a role of the anaphase-promoting complex in the turnover of Dbf4p. Overexpression of Dbf4p in the G1 phase can partially overcome this elevated turnover and lead to an increase in Cdc7p kinase activity. Thus, the regulation of Dbf4p levels through the control of Dbf4p degradation has an important role in the regulation of Cdc7p kinase activity during the cell cycle.  (+info)

Cdc20 protein contains a destruction-box but, unlike Clb2, its proteolysisis not acutely dependent on the activity of anaphase-promoting complex. (3/25)

Both chromosome segregation and the final exit from mitosis require a ubiquitin-protein ligase called anaphase-promoting complex (APC) or cyclosome. This multiprotein complex ubiquitinates various substrates, such as the anaphase inhibitor Pds1 and mitotic cyclins, and thus targets them for proteolysis by the 26S proteasome. The ubiquitination by APC is dependent on the presence of a destruction-box sequence in the N-terminus of target proteins. Recent reports have strongly suggested that Cdc20, a WD40 repeat-containing protein required for nuclear division in the budding yeast Saccharomyces cerevisiae, is essential for the APC-mediated proteolysis. To understand the function of CDC20, we have studied its regulation in some detail. The expression of the CDC20 gene is cell-cycle regulated such that it is transcribed only during late S phase and mitosis. Although the protein is unstable to some extent through out the cell cycle, its degradation is particularly enhanced in G1. Cdc20 contains a destruction box sequence which, when mutated or deleted, stabilizes it considerably in G1. Surprisingly, we find that while the inactivation of APC subunits Cdc16, Cdc23 or Cdc27 results in stabilization of the mitotic cyclin Clb2 in G1, the proteolytic destruction of Cdc20 remains largely unaffected. This suggests the existence of proteolytic mechanisms in G1 that can degrade destruction-box containing proteins, such as Cdc20, in an APC-independent manner.  (+info)

Phosphorylation by Cdc28 activates the Cdc20-dependent activity of the anaphase-promoting complex. (4/25)

Budding yeast initiates anaphase by activating the Cdc20-dependent anaphase-promoting complex (APC). The mitotic activity of Cdc28 (Cdk1) is required to activate this form of the APC, and mutants that are impaired in mitotic Cdc28 function have difficulty leaving mitosis. This defect can be explained by a defect in APC phosphorylation, which depends on mitotic Cdc28 activity in vivo and can be catalyzed by purified Cdc28 in vitro. Mutating putative Cdc28 phosphorylation sites in three components of the APC, Cdc16, Cdc23, and Cdc27, makes the APC resistant to phosphorylation both in vivo and in vitro. The nonphosphorylatable APC has normal activity in G1, but its mitotic, Cdc20-dependent activity is compromised. These results show that Cdc28 activates the APC in budding yeast to trigger anaphase. Previous reports have shown that the budding yeast Cdc5 homologue, Plk, can also phosphorylate and activate the APC in vitro. We show that, like cdc28 mutants, cdc5 mutants affect APC phosphorylation in vivo. However, although Cdc5 can phosphorylate Cdc16 and Cdc27 in vitro, this in vitro phosphorylation does not occur on in vivo sites of phosphorylation.  (+info)

Identification of EPI64, a TBC/rabGAP domain-containing microvillar protein that binds to the first PDZ domain of EBP50 and E3KARP. (5/25)

The cortical scaffolding proteins EBP50 (ERM-binding phosphoprotein-50) and E3KARP (NHE3 kinase A regulatory protein) contain two PDZ (PSD-95/DlgA/ZO-1-like) domains followed by a COOH-terminal sequence that binds to active ERM family members. Using affinity chromatography, we identified polypeptides from placental microvilli that bind the PDZ domains of EBP50. Among these are 64- and/or 65-kD differentially phosphorylated polypeptides that bind preferentially to the first PDZ domain of EBP50, as well as to E3KARP, and that we call EPI64 (EBP50-PDZ interactor of 64 kD). The gene for human EPI64 lies on chromosome 22 where nine exons specify a protein of 508 residues that contains a Tre/Bub2/Cdc16 (TBC)/rab GTPase-activating protein (GAP) domain. EPI64 terminates in DTYL, which is necessary for binding to the PDZ domains of EBP50, as a mutant ending in DTYLA no longer interacts. EPI64 colocalizes with EBP50 and ezrin in syncytiotrophoblast and cultured cell microvilli, and this localization in cultured cells is abolished by introduction of the DTYLA mutation. In addition to EPI64, immobilized EBP50 PDZ domains retain several polypeptides from placental microvilli, including an isoform of nadrin, a rhoGAP domain-containing protein implicated in regulating vesicular transport. Nadrin binds EBP50 directly, probably through its COOH-terminal STAL sequence. Thus, EBP50 appears to bind membrane proteins as well as factors potentially involved in regulating membrane traffic.  (+info)

A screen for Schizosaccharomyces pombe mutants defective in rereplication identifies new alleles of rad4+, cut9+ and psf2+. (6/25)

Fission yeast mutants defective in DNA replication have widely varying morphological phenotypes. We designed a screen for temperature-sensitive mutants defective in the process of replication regardless of morphology by isolating strains unable to rereplicate their DNA in the absence of cyclin B (Cdc13). Of the 42 rereplication-defective mutants analyzed, we were able to clone complementing plasmids for 10. This screen identified new alleles of the APC subunit cut9(+), the initiation/checkpoint factor rad4(+)/cut5(+), and the first mutant allele of psf2(+), a subunit of the novel GINS replication complex. Other genes identified are likely to play general roles in gene expression and protein localization.  (+info)

Interaction of APC/C-E3 ligase with Swi6/HP1 and Clr4/Suv39 in heterochromatin assembly in fission yeast. (7/25)

 (+info)

Insights into anaphase promoting complex TPR subdomain assembly from a CDC26-APC6 structure. (8/25)

 (+info)

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TMTC2 (transmembrane and tetratricopeptide repeat containing 2), Authors: Dessen P. Published in: Atlas Genet Cytogenet Oncol Haematol.
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April 2015). "Structure of an APC3-APC16 complex: insights into assembly of the anaphase-promoting complex/cyclosome". Journal ... "Insights into anaphase promoting complex TPR subdomain assembly from a CDC26-APC6 structure". Nature Structural & Molecular ... a Drosophila gene encoding the Cdc27 subunit of the anaphase promoting complex, enhance centrosomal defects in polo and are ... Anaphase-promoting complex (also called the cyclosome or APC/C) is an E3 ubiquitin ligase that marks target cell cycle proteins ...
2004). "The Apc5 Subunit of the Anaphase-Promoting Complex/Cyclosome Interacts with Poly(A) Binding Protein and Represses ... consists of at least 8 protein subunits, including APC5, CDC27 (APC3; MIM 116946), CDC16 (APC6; MIM 603461), and CDC23 (APC8; ... Anaphase-promoting complex subunit 5 is an enzyme that in humans is encoded by the ANAPC5 gene. The anaphase-promoting complex ... "The Apc5 Subunit of the Anaphase-Promoting Complex/Cyclosome Interacts with Poly(A) Binding Protein and Represses Internal ...
The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the ... Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls ... Anaphase-promoting complex subunit 6. Short name: APC6. CDC16 homolog. Short name: ... Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls ...
APC6), CDC16 homolog (CDC16Hs), Cyclosome subunit 6, CDC16, ANAPC6 ... Anaphase-promoting complex subunit 6 (APC6). CDC16 homolog (CDC16Hs). Cyclosome subunit 6. Gene name: CDC16, ANAPC6 Cell ... Synthetic peptide derived from internal domains of human APC6. Target Specificity Reacts with human 72 kDa APC6. Cross-reacts ...
Anapc6,Anaphase-promoting complex subunit 6,APC6,Cdc16,Cell division cycle protein 16 homolog,Cyclosome subunit 6,Mouse,Mus ... Anaphase-promoting complex subunit 2 (Cyclosome subunit 2). [cut4 apc1 SPBC106.09] Anaphase-promoting complex subunit 1 (20S ... Anaphase-promoting complex subunit 7 (APC7) (Cyclosome subunit 7). [APC8 CDC23 At3g48150 T24C20.30] Anaphase-promoting complex ... ANAPC12,Anaphase-promoting complex subunit 12,Anaphase-promoting complex subunit CDC26,APC12,C9orf17,CDC26,Cell division cycle ...
The anaphase-promoting complex/cyclosome (APC/C), an E3 ubiquitin ligase responsible for controlling cell cycle transitions, is ... APC/C; Cdc16/Cut9/Apc6; cell cycle; crystal structure; tetratricopeptide repeat/TPR;ANAPHASE-PROMOTING COMPLEX; CRYSTAL- ... Cdc16/Apc6) in complex with Hcn1 (Cdc26), showing that Cdc16/Cut9 is a contiguous TPR superhelix of 14 TPR units. A C-terminal ... STRUCTURE; FISSION YEAST; PROTEIN STRUCTURES; BUDDING YEAST; TPR PROTEINS; MITOSIS; DESTRUCTION; CDH1; COMPLEX/CYCLOSOME. ...
Human Anaphase-promoting complex subunit 6 ELISA Kit;Human APC6 ELISA Kit;Human CDC16 homolog ELISA Kit;Human CDC16Hs ELISA Kit ... Human cyclosome subunit 6 ELISA Kit;Human ANAPC6 ELISA Kit;Human CUT9 ELISA Kit;Human cell division cycle 16 ELISA Kit;Human ... Human anaphase-promoting complex, subunit 6 ELISA Kit;Human cell division cycle 16 homolog ELISA Kit;. ...
CDC23 is required at the metaphase/anaphase transition to separate sister chromatids, and we speculate that it might promote ... Proteolysis of CLB2 is initiated in early anaphase, but a fraction of CLB2 remains stable until anaphase is complete. ... Their failure to exit anaphase can be explained by defective cyclin proteolysis. ... cdc23-1 mutants are defective in both entering and exiting anaphase. ...
... anaphase-promoting complex subunit 6 , cyclosome subunit 6 , CDC16Hs , anaphase-promoting complex, subunit 6 ... 2700071J12Rik, 2810431D22Rik, ANAPC6, anaphase promoting complex 6, APC6, CUT9, F9K20.19, F9K20_19, zgc:123264 ... We report gene alterations in several components of this complex in human colon cancer cells, including APC6/CDC16 and APC8/ ... The APC complex is a cyclin degradation system that governs exit from mitosis. Each component protein of the APC complex is ...
April 2015). "Structure of an APC3-APC16 complex: insights into assembly of the anaphase-promoting complex/cyclosome". Journal ... "Insights into anaphase promoting complex TPR subdomain assembly from a CDC26-APC6 structure". Nature Structural & Molecular ... a Drosophila gene encoding the Cdc27 subunit of the anaphase promoting complex, enhance centrosomal defects in polo and are ... Anaphase-promoting complex (also called the cyclosome or APC/C) is an E3 ubiquitin ligase that marks target cell cycle proteins ...
2004). "The Apc5 Subunit of the Anaphase-Promoting Complex/Cyclosome Interacts with Poly(A) Binding Protein and Represses ... consists of at least 8 protein subunits, including APC5, CDC27 (APC3; MIM 116946), CDC16 (APC6; MIM 603461), and CDC23 (APC8; ... Anaphase-promoting complex subunit 5 is an enzyme that in humans is encoded by the ANAPC5 gene. The anaphase-promoting complex ... "The Apc5 Subunit of the Anaphase-Promoting Complex/Cyclosome Interacts with Poly(A) Binding Protein and Represses Internal ...
... anaphase-promoting complex, subunit 10 family / APC10 family ; Component of the anaphase promoting complex/cyclosome (APC/C), a ... AT1G78770 (E=2e-291) APC6 , cell division cycle family protein (544 aa) ... anaphase-promoting complex, subunit 10 family / APC10 family ; Component of the anaphase promoting complex/cyclosome (APC/C), a ... anaphase-promoting complex, subunit 10 family / APC10 family ; Component of the anaphase promoting complex/cyclosome (APC/C), a ...
The APC (anaphase-promoting complex) or cyclosome is a large multisubunit protein complex. It has 13 core components (with ... Subunits with unknown functions. Functions for the remaining APC subunits are less clear. At least one other APC subunit, Apc5 ... Cdc16/Apc6 Yes 94 TPR motifs Cdc23/Apc8 Yes 70 TPR motifs ... anaphase-promoting complex (APC), cell cycle, cyclosome, E3 ... The anaphase-promoting complex (APC): the sum of its parts? L.A. Passmore L.A. Passmore ...
Ubiquitin-Protein Ligase Complexes/physiology*. Substances. *Apc8 Subunit, Anaphase-Promoting Complex-Cyclosome ... Mean of two experiments where individual values from each independent experiment are denoted by a line (APC2, APC6, APC8), ... The Anaphase Promoting Complex/Cyclosome (APC/C) in complex with its co-activator Cdc20 is responsible for targeting proteins ... Nek2A and the mitotic checkpoint complex (MCC) have an overlap in APC/C subunit requirements for binding and we propose that ...
We exemplified this trend on the anaphase promoting complex (APC) where a core is highly conserved throughout all metazoans, ... They do not act alone but are organised in complexes. Throughout the life of a cell, complexes are dynamic in their composition ... Focussing on human protein complexes, we based our analysis on a manually curated dataset from HPRD. In total, 1,060 complexes ... of all complexes affected). Still, loss of whole complexes happened rarely. This biological signal deviated significantly from ...
Anaphase Promoting Complex Subunit 4, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards ... Anaphase-Promoting Complex Subunit 4 3 4 * Cyclosome Subunit 4 3 4 ... The mammalian APC/C is composed at least of 14 distinct subunits ANAPC1, ANAPC2, CDC27/APC3, ANAPC4, ANAPC5, CDC16/APC6, ANAPC7 ... A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition ...
Anaphase Promoting Complex Subunit 5, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards ... Cyclosome subunit 5 (APC5_HUMAN). *cDNA FLJ55537, highly similar to Anaphase-promoting complex subunit 5 (APC5)(Cyclosome ... The mammalian APC/C is composed at least of 14 distinct subunits ANAPC1, ANAPC2, CDC27/APC3, ANAPC4, ANAPC5, CDC16/APC6, ANAPC7 ... cDNA FLJ30217 fis, clone BRACE2001709, highly similar to Anaphase-promoting complex subunit 5 (APC5)(Cyclosome subunit 5) ( ...
Anaphase-promoting complex-cyclosome; Adenocarcinoma; Colorectal neoplasms; Cell cycle INTRODUCTION. The anaphase-promoting ... TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1. Curr Biol 2003;13:1459-1468. ... 16] reported a genetic alteration in APC6 and APC8 in human colon cancer cells and suggested their involvement in colon ... Polyubiquitin chains are added to securin by an E3 ubiquitin ligase known as anaphase-promoting complex or cyclosome. If ...
The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the ... Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls ... Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls ... The mammalian APC/C is composed at least of 14 distinct subunits ANAPC1, ANAPC2, CDC27/APC3, ANAPC4, ANAPC5, CDC16/APC6, ANAPC7 ...
Anaphase-promoting complex/cyclosome (APC/C) is a multifunctional ubiquitin-protein ligase that targets different substrates ... b A schematic illustration of the structure organization of APC/C complex. APC/C complex contains three sub-complexes: the ... Structure and genetics characteristics of APC/C. a Graphic representation of human (Homo sapiens) APC/C subunits. All domains ... The TPR lobe consists of APC3, APC6, APC8, APC7, APC13, APC16, and Cdc26. The scaffolding platform connects the TPR lobe to the ...
The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the ... Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls ... "Identification of a cullin homology region in a subunit of the anaphase-promoting complex.". Yu H., Peters J.-M., King R.W., ... Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls ...
1998 Regulation of the anaphase-promoting complex/cyclosome by bimAAPC3 and proteolysis of NIMA. Mol. Biol. Cell 9: 3019-3030. ... Similar phenotypes are associated with mutations in the APC/C subunits BIMEAPC1 and BIMHAPC6 (Lieset al. 1998; P. M. Mirabito, ... Here we show that sepI1 is an allele of bimAAPC3, which encodes a component of the anaphase-promoting complex/cyclosome (APC/C ... 1997 MAD2 associates with the cyclosome/anaphase-promoting complex and inhibits its activity. Proc. Natl. Acad. Sci. USA 94: ...
The anaphase-promoting complex/cyclosome, APC/C, the DYRK (Dual-specificity tyrosine-regulated kinase), MBK-2, and the CUL-2- ... We asked if the APC-6/CDC16 subunit gene emb-27 acts in parallel or strictly sequentially with mbk-2. This analysis requires ... 2002 Multiple subunits of the Caenorhabditis elegans anaphase-promoting complex are required for chromosome segregation during ... mediated MEI-1 degradation pathway is activated upon the completion of meiosis by the anaphase promoting complex/cyclosome (APC ...
2009) Structure of the anaphase-promoting complex/cyclosome interacting with a mitotic checkpoint complex. Science 323:1477- ... 2003) TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1. Curr. Biol. 13:1459-1468. ... Cdc16/APC6 (1∶1000; SC‐6395, Santa Cruz Biotechnology), rabbit anti‐APC10 (611501/2, Biolegend), mouse anti‐BubR1 (Chemicon ... 2006) The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol. 7:644-656, doi:10.1038 ...
Apc6 Subunit, Anaphase-Promoting Complex-Cyclosome. Subunidade Apc6 do Ciclossomo-Complexo Promotor de Anáfase. Subunidad Apc6 ... Apc6 Subunit, Anaphase-Promoting Complex-Cyclosome. Subunidade Apc6 do Ciclossomo-Complexo Promotor de Anáfase. Subunidad Apc6 ... Apc1 Subunit, Anaphase-Promoting Complex-Cyclosome. Subunidade Apc1 do Ciclossomo-Complexo Promotor de Anáfase. Subunidad Apc1 ... Apc2 Subunit, Anaphase-Promoting Complex-Cyclosome. Subunidade Apc2 do Ciclossomo-Complexo Promotor de Anáfase. Subunidad Apc2 ...
... the three TPR subunits in the head region of the APC/C assemble in an ordered fashion: in yeast, APC6/Cdc16 is needed for ... APC16 is a conserved subunit of the anaphase-promoting complex/cyclosome. Geert J. P. L. Kops, Monique van der Voet, Michael S ... APC16 is a conserved subunit of the anaphase-promoting complex/cyclosome. Geert J. P. L. Kops, Monique van der Voet, Michael S ... APC16 is a conserved subunit of the anaphase-promoting complex/cyclosome. Geert J. P. L. Kops, Monique van der Voet, Michael S ...
Inactivation of PKA is important for the activation of the cyclosome for promoting anaphase, perhaps through dephosphorylation ... of the subunits such as Cut9 (Apc6).. abbotic forte syrup swimwear. Acute, unstable, slipped capital femoral epiphysis: is ... For our study, we explored the structural connectivity of the MD thalamic nuclei and the CM/Pf complex towards five cortical ... The expression of cyclin A (a regulatory subunit of cdk2) markedly decreased, while cyclin D1, the major cdk4 partner in ...
  • Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. (uniprot.org)
  • The anaphase-promoting complex/cyclosome (APC/C), an E3 ubiquitin ligase responsible for controlling cell cycle transitions, is a multisubunit complex assembled from 13 different proteins. (icr.ac.uk)
  • Thus, in complex with Cdc16/Cut9, the N-acetyl-Met residue of Hcn1, a putative degron for the Doa10 E3 ubiquitin ligase, is inaccessible for Doa10 recognition, protecting Hcn1/Cdc26 from ubiquitin-dependent degradation. (icr.ac.uk)
  • This gene encodes a component protein of the APC complex, which is composed of eight proteins and functions as a protein ubiquitin ligase. (antikoerper-online.de)
  • The APC (anaphase-promoting complex) is a multisubunit E3 ubiquitin ligase that targets cell-cycle-related proteins for degradation by the 26 S proteasome. (portlandpress.com)
  • This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. (genecards.org)
  • The anaphase-promoting complex (APC) is a multiprotein complex with E3 ubiquitin ligase activity and is required for ubiquitination of securin and cyclin-B. Several APC-targeting molecules are reported to be oncogenes. (coloproctol.org)
  • The anaphase-promoting complex (APC) is an E3 ubiquitin ligase that controls mitotic progression [ 1 2 ]. (coloproctol.org)
  • The anaphase-promoting complex/cyclosome, APC/C, the DYRK (Dual-specificity tyrosine-regulated kinase), MBK-2 , and the CUL-2 -based E3 ubiquitin ligase act together to degrade MEI-1 , in parallel to MEL-26 / CUL-3 . (g3journal.org)
  • The major pathway involves MEL-26 (maternal effect lethal, Dow and Mains 1998 ) as a substrate recognition subunit that recruits MEI-1 to the CUL-3 /cullin-based E3 ubiquitin ligase. (g3journal.org)
  • The anaphase‐promoting complex/cyclosome (APC/C) is an E3 ubiquitin ligase that, together with either one of its regulatory co‐activators, Cdc20 or Cdh1, targets multiple mitotic regulators for proteasomal degradation. (biologists.org)
  • Error-free chromosome segregation depends on timely activation of the multi-subunit E3 ubiquitin ligase APC/C. Activation of the APC/C initiates chromosome segregation and mitotic exit by targeting critical cell-cycle regulators for destruction. (biologists.org)
  • One of the machineries that promotes protein destruction is the E3 ubiquitin ligase anaphase-promoting complex/cyclosome or APC/C ( Peters, 2006 ). (biologists.org)
  • CDC27Hs colocalizes with CDC16Hs to the centrosome and mitotic spindle and is essential for the metaphase to anaphase transition. (antibody-antibodies.com)
  • The serine/threonine phosphatase PP5 interacts with CDC16 and CDC27, two tetratricopeptide repeat-containing subunits of the anaphase-promoting complex. (antibody-antibodies.com)
  • Numerous APC/C subunits incorporate multiple copies of the tetratricopeptide repeat (TPR). (icr.ac.uk)
  • This protein and two other APC complex proteins, CDC23 and CDC27, contain a tetratricopeptide repeat (TPR), a protein domain that may be involved in protein-protein interaction. (antikoerper-online.de)
  • APC is a polymeric protein complex composed of at least 11 subunits and contains tetratricopeptide repeat proteins (APC3, 5, 6, 7, and 8), a cullin homolog (APC2), and a ring-H2 finger domain (APC11). (coloproctol.org)
  • Here, we report the crystal structure of Schizosaccharomyces pombe Cut9 (Cdc16/Apc6) in complex with Hcn1 (Cdc26), showing that Cdc16/Cut9 is a contiguous TPR superhelix of 14 TPR units. (icr.ac.uk)
  • We report gene alterations in several components of this complex in human colon cancer cells, including APC6/CDC16 and APC8/CDC23 (zeige CDC23 Antikörper ) which are known to be key function elements. (antikoerper-online.de)
  • The large complexes involved in transcription (polymerases and transcription factors) and translation (ribosomes) must be tightly regulated to allow the control of gene expression. (portlandpress.com)
  • ANAPC4 (Anaphase Promoting Complex Subunit 4) is a Protein Coding gene. (genecards.org)
  • CDC23 is required at the metaphase/anaphase transition to separate sister chromatids, and we speculate that it might promote proteolysis of proteins that hold sister chromatids together. (ox.ac.uk)
  • A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. (genecards.org)
  • Recently, it has been shown that cyclin A destruction early in mitosis is necessary for progressive stabilization of the mitotic spindle, promoting proper attachments to kinetochores and formation of the metaphase plate ( Kabeche and Compton, 2013 ). (biologists.org)
  • Proteolysis of CLB2 is initiated in early anaphase, but a fraction of CLB2 remains stable until anaphase is complete. (ox.ac.uk)
  • The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. (uniprot.org)
  • This finding may provide a structural explanation for a mechanism to control the stoichiometry of proteins participating in multisubunit complexes. (icr.ac.uk)
  • The APC contains at least 13 subunits and is regulated by the binding of co-activator proteins and by phosphorylation. (portlandpress.com)
  • Most cellular proteins form a complex network of interactions with other proteins and many are components of large multiprotein complexes [ 1 - 3 ]. (portlandpress.com)
  • The Anaphase Promoting Complex/Cyclosome (APC/C) in complex with its co-activator Cdc20 is responsible for targeting proteins for ubiquitin-mediated degradation during mitosis. (nih.gov)
  • Here, the complexes are connected by shared components, e.g. proteins present in more than one complex. (beds.ac.uk)
  • Some of these attachments, which can consist of multiple proteins itself, can be connected to different core complexes. (beds.ac.uk)
  • Nek2 isoform A (Nek2A) is a presumed substrate of the anaphase‐promoting complex/cyclosome containing Cdc20 (APC/C Cdc20 ). (biologists.org)
  • A ) The APC/C complex was affinity purified using an APC4 antibody from nocodazole-arrested cells or cells released from nocodazole into MG132 for 2 h. (nih.gov)
  • C ) Stable HeLa cell lines expressing FLAG-tagged Kif18A or Kif18AΔLR were arrested with nocodazole and the APC/C complex purified using an APC4 antibody. (nih.gov)
  • Mad2 with the cyclosome/anaphase-promoting complex, and is involved in regulating anaphase onset and late mitotic events. (antibody-antibodies.com)
  • Why do large stable multiprotein complexes exist? (portlandpress.com)
  • Intraflagellar transport (IFT) particles are multiprotein complexes that move bidirectionally along the cilium/flagellum. (courtfield.tk)
  • Over the analysed species, the composition of most complexes was highly flexible and only 25% of all genes were never lost. (beds.ac.uk)
  • These evolutionary events affecting genes coding for units in human protein complexes showed a significantly different phylogenetic pattern compared to randomly selected genes. (beds.ac.uk)
  • Alterations of anaphase-promoting complex genes in human colon cancer cells. (uniprot.org)
  • The APC complex is a cyclin degradation system that governs exit from mitosis. (antikoerper-online.de)
  • The proteolytic events triggered by the APC are required to release sister chromatid cohesion during anaphase, to control the exit from mitosis and to prevent premature entry into S-phase. (portlandpress.com)
  • The APC (anaphase-promoting complex) or cyclosome is a large multisubunit protein complex. (portlandpress.com)
  • The MEI-1 / MEI-2 katanin microtubule-severing complex is essential for meiotic spindle formation but must be quickly inactivated to allow for proper formation of the mitotic spindle. (g3journal.org)
  • At the point in the cell cycle when APC/C Cdc20 complexes are formed, however, the spindle checkpoint also becomes active and blocks Cdc20. (biologists.org)
  • We now report the identification and characterization of APC16, a conserved subunit of the APC/C. APC16 was found in association with tandem-affinity-purified mitotic checkpoint complex protein complexes. (biologists.org)
  • APC16 is a bona fide subunit of human APC/C: it is present in APC/C complexes throughout the cell cycle, the phenotype of APC16-depleted cells copies depletion of other APC/C subunits, and APC16 is important for APC/C activity towards mitotic substrates. (biologists.org)
  • Their failure to exit anaphase can be explained by defective cyclin proteolysis. (ox.ac.uk)
  • First, enhancing mitotic checkpoint complex (MCC) formation by nocodazole treatment inhibited the degradation of geminin and cyclin A, whereas Nek2A disappeared at a normal rate. (biologists.org)
  • Mechanisms controlling the temporal degradation of Nek2A and Kif18A by the APC/C-Cdc20 complex. (nih.gov)
  • We find that dimerization via the leucine zipper, in combination with the MR motif, is required for stable Nek2A binding to and ubiquitination by the APC/C. Nek2A and the mitotic checkpoint complex (MCC) have an overlap in APC/C subunit requirements for binding and we propose that Nek2A binds with high affinity to apo-APC/C and is degraded by the pool of Cdc20 that avoids inhibition by the SAC. (nih.gov)
  • Kif18A is ubiquitinated by the APC/C-Cdc20 complex. (nih.gov)
  • It is not known why the APC contains 13 subunits when many other ubiquitin ligases are small single-subunit enzymes. (portlandpress.com)
  • Although the activity and specificity of E3s such as the APC are crucial, most E3s exist as much smaller, often single-subunit, enzymes. (portlandpress.com)
  • Each component protein of the APC complex is highly conserved among eukaryotic organisms. (antikoerper-online.de)
  • We exemplified this trend on the anaphase promoting complex (APC) where a core is highly conserved throughout all metazoans, but flexibility in certain components is observable. (beds.ac.uk)
  • Reacts with human 72 kDa APC6. (covalab.com)
  • Mitotic regulation of the human anaphase-promoting complex by phosphorylation. (antibody-antibodies.com)
  • Mechanism of ubiquitin-chain formation by the human anaphase-promoting complex. (antibody-antibodies.com)
  • Focussing on human protein complexes, we based our analysis on a manually curated dataset from HPRD. (beds.ac.uk)
  • Structure and genetics characteristics of APC/C. a Graphic representation of human ( Homo sapiens ) APC/C subunits. (biomedcentral.com)
  • However in contrast to Nek2A, Kif18A is not degraded until anaphase showing that additional mechanisms contribute to Nek2A degradation. (nih.gov)
  • In this review, I discuss the composition of the APC and the functions of its subunits with the goal of gaining insight into the mechanism of the APC as a whole. (portlandpress.com)
  • Throughout the life of a cell, complexes are dynamic in their composition due to attachments and shared components. (beds.ac.uk)
  • We computed interologs in 25 different species and predicted the composition of complexes. (beds.ac.uk)
  • cdc23-1 mutants are defective in both entering and exiting anaphase. (ox.ac.uk)
  • Thus, protein complexes contain not only structural and functional, but also evolutionary cores. (beds.ac.uk)
  • This complexity, comprising both the functional and structural entities of protein complexes, raises the question how the interplay of core complexes with variable attachments evolved. (beds.ac.uk)
  • Even if one component was lost at a particular point in time, the fraction of observed second, independent losses of additional components was high (75% of all complexes affected). (beds.ac.uk)
  • In the present study, the structures and functions of individual APC subunits are discussed. (portlandpress.com)
  • Experimental and computational evidence indicate that consecutive addition and secondary losses of components played a major role in the evolution of some complexes, mostly without affecting the core function. (beds.ac.uk)
  • The combination of core functional units with variably attached modules increases the number of different complexes and thereby the complexity of the cell. (beds.ac.uk)
  • Consecutive additions and losses of distinct units is a fundamental process in the evolution of protein complexes. (beds.ac.uk)