An E3 ubiquitin ligase primarily involved in regulation of the metaphase-to-anaphase transition during MITOSIS through ubiquitination of specific CELL CYCLE PROTEINS. Enzyme activity is tightly regulated through subunits and cofactors, which modulate activation, inhibition, and substrate specificity. The anaphase-promoting complex, or APC-C, is also involved in tissue differentiation in the PLACENTA, CRYSTALLINE LENS, and SKELETAL MUSCLE, and in regulation of postmitotic NEURONAL PLASTICITY and excitability.
Ubiquitin-Protein Ligase Complexes
Complexes of enzymes that catalyze the covalent attachment of UBIQUITIN to other proteins by forming a peptide bond between the C-terminal GLYCINE of UBIQUITIN and the alpha-amino groups of LYSINE residues in the protein. The complexes play an important role in mediating the selective-degradation of short-lived and abnormal proteins. The complex of enzymes can be broken down into three components that involve activation of ubiquitin (UBIQUITIN-ACTIVATING ENZYMES), conjugation of ubiquitin to the ligase complex (UBIQUITIN-CONJUGATING ENZYMES), and ligation of ubiquitin to the substrate protein (UBIQUITIN-PROTEIN LIGASES).
Highly conserved proteins that specifically bind to and activate the anaphase-promoting complex-cyclosome, promoting ubiquitination and proteolysis of cell-cycle-regulatory proteins. Cdc20 is essential for anaphase-promoting complex activity, initiation of anaphase, and cyclin proteolysis during mitosis.
Apc3 Subunit, Anaphase-Promoting Complex-Cyclosome
A highly evolutionarily conserved subunit of the anaphase-promoting complex (APC-C) containing multiple 34-amino-acid tetratricopeptide repeats. These domains, also found in Apc subunits 6, 7, and 8, have been shown to mediate protein-protein interactions, suggesting that Apc3 may assist in coordinating the juxtaposition of the catalytic and substrate recognition module subunits relative to co-activators and APC-C inhibitors.
Cell Cycle Proteins
Proteins that control the CELL DIVISION CYCLE. This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS.
Apc1 Subunit, Anaphase-Promoting Complex-Cyclosome
The largest subunit of the anaphase-promoting complex. It acts primarily as a scaffold for the proper organization and arrangement of subunits. The C-terminal region of Apc1 contains a series of tandem amino acid repeats that are also seen in the 26S proteasome regulatory particle, and may assist with forming and stabilizing protein-protein interactions.
Securin is involved in the control of the metaphase-anaphase transition during MITOSIS. It promotes the onset of anaphase by blocking SEPARASE function and preventing proteolysis of cohesin and separation of sister CHROMATIDS. Overexpression of securin is associated with NEOPLASTIC CELL TRANSFORMATION and tumor formation.
A family of proteins that share the F-BOX MOTIF and are involved in protein-protein interactions. They play an important role in process of protein ubiquition by associating with a variety of substrates and then associating into SCF UBIQUITIN LIGASE complexes. They are held in the ubiquitin-ligase complex via binding to SKP DOMAIN PROTEINS.
M Phase Cell Cycle Checkpoints
Apc5 Subunit, Anaphase-Promoting Complex-Cyclosome
Mad2 is a component of the spindle-assembly checkpoint apparatus. It binds to and inhibits the Cdc20 activator subunit of the anaphase-promoting complex, preventing the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Mad2 is required for proper microtubule capture at KINETOCHORES.
A diverse class of enzymes that interact with UBIQUITIN-CONJUGATING ENZYMES and ubiquitination-specific protein substrates. Each member of this enzyme group has its own distinct specificity for a substrate and ubiquitin-conjugating enzyme. Ubiquitin-protein ligases exist as both monomeric proteins multiprotein complexes.
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
CDC2 Protein Kinase
Phosphoprotein with protein kinase activity that functions in the G2/M phase transition of the CELL CYCLE. It is the catalytic subunit of the MATURATION-PROMOTING FACTOR and complexes with both CYCLIN A and CYCLIN B in mammalian cells. The maximal activity of cyclin-dependent kinase 1 is achieved when it is fully dephosphorylated.
Schizosaccharomyces pombe Proteins
Apc2 Subunit, Anaphase-Promoting Complex-Cyclosome
Together with the Apc11 subunit, forms the catalytic core of the E3 ubiquitin ligase anaphase-promoting complex (APC-C). Its N-terminus has cullin domains which associate with the RING FINGER DOMAINS of Apc11. Apc2 also interacts with the E2 ubiquitin ligases involved in APC-C ubiquitination reactions.
Saccharomyces cerevisiae Proteins
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Apc8 Subunit, Anaphase-Promoting Complex-Cyclosome
A highly conserved subunit of the anaphase-promoting complex (APC-C) containing multiple 34-amino-acid tetratricopeptide repeats. These domains, also found in Apc3, Apc6, and Apc7, have been shown to mediate protein-protein interactions, suggesting that Apc8 may assist in coordinating the juxtaposition of the catalytic and substrate recognition module subunits relative to coactivators and APC-C inhibitors.
Amino Acid Sequence
A family of highly conserved serine-threonine kinases that are involved in the regulation of MITOSIS. They are involved in many aspects of cell division, including centrosome duplication, SPINDLE APPARATUS formation, chromosome alignment, attachment to the spindle, checkpoint activation, and CYTOKINESIS.
Either of the two longitudinally adjacent threads formed when a eukaryotic chromosome replicates prior to mitosis. The chromatids are held together at the centromere. Sister chromatids are derived from the same chromosome. (Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Proto-Oncogene Proteins c-mos
A highly conserved 76-amino acid peptide universally found in eukaryotic cells that functions as a marker for intracellular PROTEIN TRANSPORT and degradation. Ubiquitin becomes activated through a series of complicated steps and forms an isopeptide bond to lysine residues of specific proteins within the cell. These "ubiquitinated" proteins can be recognized and degraded by proteosomes or be transported to specific compartments within the cell.
Apc6 Subunit, Anaphase-Promoting Complex-Cyclosome
A highly conserved subunit of the anaphase-promoting complex (APC-C) containing multiple 34 amino acid tetratricopeptide repeats. These domains, also found in Apc3, Apc7, and Apc8, have been shown to mediate protein-protein interactions, suggesting that Apc6 may assist in coordinating the juxtaposition of the catalytic and substrate recognition module subunits relative to coactivators and APC-C inhibitors.
SKP Cullin F-Box Protein Ligases
Proteasome Endopeptidase Complex
A large multisubunit complex that plays an important role in the degradation of most of the cytosolic and nuclear proteins in eukaryotic cells. It contains a 700-kDa catalytic sub-complex and two 700-kDa regulatory sub-complexes. The complex digests ubiquitinated proteins and protein activated via ornithine decarboxylase antizyme.
A gene silencing phenomenon whereby specific dsRNAs (RNA, DOUBLE-STRANDED) trigger the degradation of homologous mRNA (RNA, MESSENGER). The specific dsRNAs are processed into SMALL INTERFERING RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA-INDUCED SILENCING COMPLEX. DNA METHYLATION may also be triggered during this process.
Recombinant Fusion Proteins
Amino Acid Motifs
Apc11 Subunit, Anaphase-Promoting Complex-Cyclosome
Adenomatous Polyposis Coli Protein
Calcium-dependent cell adhesion proteins. They are important in the formation of ADHERENS JUNCTIONS between cells. Cadherins are classified by their distinct immunological and tissue specificities, either by letters (E- for epithelial, N- for neural, and P- for placental cadherins) or by numbers (cadherin-12 or N-cadherin 2 for brain-cadherin). Cadherins promote cell adhesion via a homophilic mechanism as in the construction of tissues and of the whole animal body.
Protein Processing, Post-Translational
Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.
RNA, Small Interfering
Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.
Chromosomal Proteins, Non-Histone
Apc7 Subunit, Anaphase-Promoting Complex-Cyclosome
A highly conserved subunit of the anaphase-promoting complex (APC-C) containing multiple 34 amino acid tetratricopeptide repeats. These domains, also found in Apc3, Apc6, and Apc8, have been shown to mediate protein-protein interactions, suggesting that Apc7 may assist in coordinating the juxtaposition of the catalytic and substrate recognition module subunits relative to coactivators and APC-C inhibitors.
Sequence Homology, Amino Acid
A microtubule-associated mechanical adenosine triphosphatase, that uses the energy of ATP hydrolysis to move organelles along microtubules toward the plus end of the microtubule. The protein is found in squid axoplasm, optic lobes, and in bovine brain. Bovine kinesin is a heterotetramer composed of two heavy (120 kDa) and two light (62 kDa) chains. EC 3.6.1.-.
A microtubule subunit protein found in large quantities in mammalian brain. It has also been isolated from SPERM FLAGELLUM; CILIA; and other sources. Structurally, the protein is a dimer with a molecular weight of approximately 120,000 and a sedimentation coefficient of 5.8S. It binds to COLCHICINE; VINCRISTINE; and VINBLASTINE.
Aurora Kinase B
Protein Structure, Tertiary
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Apc10 Subunit, Anaphase-Promoting Complex-Cyclosome
The cell center, consisting of a pair of CENTRIOLES surrounded by a cloud of amorphous material called the pericentriolar region. During interphase, the centrosome nucleates microtubule outgrowth. The centrosome duplicates and, during mitosis, separates to form the two poles of the mitotic spindle (MITOTIC SPINDLE APPARATUS).
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Adenomatous Polyposis Coli
Two-Hybrid System Techniques
Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Cell Cycle Checkpoints
Regulatory signaling systems that control the progression through the CELL CYCLE. They ensure that the cell has completed, in the correct order and without mistakes, all the processes required to replicate the GENOME and CYTOPLASM, and divide them equally between two daughter cells. If cells sense they have not completed these processes or that the environment does not have the nutrients and growth hormones in place to proceed, then the cells are restrained (or "arrested") until the processes are completed and growth conditions are suitable.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Gene Expression Regulation, Fungal
Fluorescent Antibody Technique
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Green Fluorescent Proteins
Within most types of eukaryotic CELL NUCLEUS, a distinct region, not delimited by a membrane, in which some species of rRNA (RNA, RIBOSOMAL) are synthesized and assembled into ribonucleoprotein subunits of ribosomes. In the nucleolus rRNA is transcribed from a nucleolar organizer, i.e., a group of tandemly repeated chromosomal genes which encode rRNA and which are transcribed by RNA polymerase I. (Singleton & Sainsbury, Dictionary of Microbiology & Molecular Biology, 2d ed)
Electrophoresis, Polyacrylamide Gel
Protein Phosphatase 2
A phosphoprotein phosphatase subtype that is comprised of a catalytic subunit and two different regulatory subunits. At least two genes encode isoforms of the protein phosphatase catalytic subunit, while several isoforms of regulatory subunits exist due to the presence of multiple genes and the alternative splicing of their mRNAs. Protein phosphatase 2 acts on a broad variety of cellular proteins and may play a role as a regulator of intracellular signaling processes.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Protein Tyrosine Phosphatases
The chromosomal constitution of cells which deviate from the normal by the addition or subtraction of CHROMOSOMES, chromosome pairs, or chromosome fragments. In a normally diploid cell (DIPLOIDY) the loss of a chromosome pair is termed nullisomy (symbol: 2N-2), the loss of a single chromosome is MONOSOMY (symbol: 2N-1), the addition of a chromosome pair is tetrasomy (symbol: 2N+2), the addition of a single chromosome is TRISOMY (symbol: 2N+1).
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Sister Chromatid Exchange
An exchange of segments between the sister chromatids of a chromosome, either between the sister chromatids of a meiotic tetrad or between the sister chromatids of a duplicated somatic chromosome. Its frequency is increased by ultraviolet and ionizing radiation and other mutagenic agents and is particularly high in BLOOM SYNDROME.
An order of the class Insecta. Wings, when present, number two and distinguish Diptera from other so-called flies, while the halteres, or reduced hindwings, separate Diptera from other insects with one pair of wings. The order includes the families Calliphoridae, Oestridae, Phoridae, SARCOPHAGIDAE, Scatophagidae, Sciaridae, SIMULIIDAE, Tabanidae, Therevidae, Trypetidae, CERATOPOGONIDAE; CHIRONOMIDAE; CULICIDAE; DROSOPHILIDAE; GLOSSINIDAE; MUSCIDAE; TEPHRITIDAE; and PSYCHODIDAE. The larval form of Diptera species are called maggots (see LARVA).
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Nuclear Matrix-Associated Proteins
A multi-functional catenin that participates in CELL ADHESION and nuclear signaling. Beta catenin binds CADHERINS and helps link their cytoplasmic tails to the ACTIN in the CYTOSKELETON via ALPHA CATENIN. It also serves as a transcriptional co-activator and downstream component of WNT PROTEIN-mediated SIGNAL TRANSDUCTION PATHWAYS.
Vacuolar Proton-Translocating ATPases
A heterogeneous group of immunocompetent cells that mediate the cellular immune response by processing and presenting antigens to the T-cells. Traditional antigen-presenting cells include MACROPHAGES; DENDRITIC CELLS; LANGERHANS CELLS; and B-LYMPHOCYTES. FOLLICULAR DENDRITIC CELLS are not traditional antigen-presenting cells, but because they hold antigen on their cell surface in the form of IMMUNE COMPLEXES for B-cell recognition they are considered so by some authors.
Identification of human APC10/Doc1 as a subunit of anaphase promoting complex. (1/17)Anaphase-promoting complex or cyclosome (APC) is a ubiquitin ligase which specifically targets mitotic regulatory factors such as Pds1/Cut2 and cyclin B. Identification of the subunits of multiprotein complex APC in several species revealed the highly conserved composition of APC from yeast to human. It has been reported, however, that vertebrate APC is composed of at least eight subunits, APC1 to APC8, while budding yeast APC is constituted of at least 12 components, Apc1 to Apc13. It has not yet been clearly understood whether additional components found in budding yeast, Apc9 to Apc13, are actually composed of mammalian APC. Here we isolated and characterized human APC10/Doc1, and found that APC10/Doc1 binds to APC core subunits throughout the cell cycle. Further, it was found that APC10/Doc1 is localized in centrosomes and mitotic spindles throughout mitosis, while it is also localized in kinetochores from prophase to anaphase and in midbody in telophase and cytokinesis. These results strongly support the notion that human APC10/Doc1 may be one of the APC core subunits rather than the transiently associated regulatory factor. (+info)
Control of metaphase-anaphase progression by proteolysis: cyclosome function regulated by the protein kinase A pathway, ubiquitination and localization. (2/17)Ubiquitin-mediated proteolysis is fundamental to cell cycle progression. In the fission yeast Schizosaccharomyces pombe, a mitotic cyclin (Cdc13), a key cell cycle regulator, is degraded for exiting mitosis, while Cut2 has to be destroyed for the onset of sister chromatid separation in anaphase. Ubiquitination of these proteins requires the special destruction box (DB) sequences locating in their N-termini and the large, 20S complex called the anaphase-promoting complex or cyclosome. Here we show that cyclosome function during metaphase-anaphase progression is regulated by the protein kinase A (PKA) inactivation pathway, ubiquitination of the cyclosome subunit, and cellular localization of the target substrates. Evidence is provided that the cyclosome plays pleiotropic roles in the cell cycle: mutations in the subunit genes show a common anaphase defect, but subunit-specific phenotypes such as in G1/S or G2/M transition, septation and cytokinesis, stress response and heavy metal sensitivity, are additionally produced, suggesting that different subunits take distinct parts of complex cyclosome functions. Inactivation of PKA is important for the activation of the cyclosome for promoting anaphase, perhaps through dephosphorylation of the subunits such as Cut9 (Apc6). Cut4 (Apc1), the largest subunit, plays an essential role in the assembly and functional regulation of the cyclosome in response to cell cycle arrest and stresses. Cut4 is highly modified, probably by ubiquitination, when it is not assembled into the 20S cyclosome. Sds23 is implicated in DB-mediated ubiquitination possibly through regulating de-ubiquitination, while Cut8 is necessary for efficient proteolysis of Cdc13 and Cut2 coupled with cytokinesis. Unexpectedly, the timing of proteolysis is dependent on cellular localization of the substrate. Cdc13 enriched along the spindle disappears first, followed by decay of the nuclear signal, whereas Cut2 in the nucleus disappears first, followed by decline in the spindle signal during metaphase-anaphase progression. (+info)
TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1. (3/17)BACKGROUND: Chromosome segregation and mitotic exit depend on activation of the anaphase-promoting complex (APC) by the substrate adaptor proteins CDC20 and CDH1. The APC is a ubiquitin ligase composed of at least 11 subunits. The interaction of APC2 and APC11 with E2 enzymes is sufficient for ubiquitination reactions, but the functions of most other subunits are unknown. RESULTS: We have biochemically characterized subcomplexes of the human APC. One subcomplex, containing APC2/11, APC1, APC4, and APC5, can assemble multiubiquitin chains but is unable to bind CDH1 and to ubiquitinate substrates. The other subcomplex contains all known APC subunits except APC2/11. This subcomplex can recruit CDH1 but fails to support any ubiquitination reaction. In vitro, the C termini of CDC20 and CDH1 bind to the closely related TPR subunits APC3 and APC7. Homology modeling predicts that these proteins are similar in structure to the peroxisomal import receptor PEX5, which binds cargo proteins via their C termini. APC activation by CDH1 depends on a conserved C-terminal motif that is also found in CDC20 and APC10. CONCLUSIONS: APC1, APC4, and APC5 may connect APC2/11 with TPR subunits. TPR domains in APC3 and APC7 recruit CDH1 to the APC and may thereby bring substrates into close proximity of APC2/11 and E2 enzymes. In analogy to PEX5, the different TPR subunits of the APC might function as receptors that interact with the C termini of regulatory proteins such as CDH1, CDC20, and APC10. (+info)
Mitotic regulation of the human anaphase-promoting complex by phosphorylation. (4/17)The anaphase-promoting complex (APC) or cyclosome is a ubiquitin ligase that initiates anaphase and mitotic exit. APC activation is thought to depend on APC phosphorylation and Cdc20 binding. We have identified 43 phospho-sites on APC of which at least 34 are mitosis specific. Of these, 32 sites are clustered in parts of Apc1 and the tetratricopeptide repeat (TPR) subunits Cdc27, Cdc16, Cdc23 and Apc7. In vitro, at least 15 of the mitotic phospho-sites can be generated by cyclin-dependent kinase 1 (Cdk1), and 3 by Polo-like kinase 1 (Plk1). APC phosphorylation by Cdk1, but not by Plk1, is sufficient for increased Cdc20 binding and APC activation. Immunofluorescence microscopy using phospho-antibodies indicates that APC phosphorylation is initiated in prophase during nuclear uptake of cyclin B1. In prometaphase phospho-APC accumulates on centrosomes where cyclin B ubiquitination is initiated, appears throughout the cytosol and disappears during mitotic exit. Plk1 depletion neither prevents APC phosphorylation nor cyclin A destruction in vivo. These observations imply that APC activation is initiated by Cdk1 already in the nuclei of late prophase cells. (+info)
The viral protein Apoptin associates with the anaphase-promoting complex to induce G2/M arrest and apoptosis in the absence of p53. (5/17)The chicken anemia virus protein Apoptin induces apoptosis in the absence of p53 by a mechanism that remains to be elucidated. Here we show that in transformed cells, Apoptin is associated with APC1, a subunit of the anaphase-promoting complex/cyclosome (APC/C). We demonstrate that Apoptin expression, or depletion of APC1 by RNA interference, inhibits APC/C function in p53 null cells, resulting in G2/M arrest and apoptosis. Our results explain the ability of Apoptin to induce apoptosis in the absence of p53 and suggest that the APC/C is an attractive target for anticancer drug development. (+info)
Apoptin nucleocytoplasmic shuttling is required for cell type-specific localization, apoptosis, and recruitment of the anaphase-promoting complex/cyclosome to PML bodies. (6/17)The chicken anemia virus protein Apoptin selectively induces apoptosis in transformed cells while leaving normal cells intact. This selectivity is thought to be largely due to cell type-specific localization: Apoptin is cytoplasmic in primary cells and nuclear in transformed cells. The basis of Apoptin cell type-specific localization and activity remains to be determined. Here we show that Apoptin is a nucleocytoplasmic shuttling protein whose localization is mediated by an N-terminal nuclear export signal (NES) and a C-terminal nuclear localization signal (NLS). Both signals are required for cell type-specific localization, since Apoptin fragments containing either the NES or the NLS fail to differentially localize in transformed and primary cells. Significantly, cell type-specific localization can be conferred in trans by coexpression of the two separate fragments, which interact through an Apoptin multimerization domain. We have previously shown that Apoptin interacts with the APC1 subunit of the anaphase-promoting complex/cyclosome (APC/C), resulting in G(2)/M cell cycle arrest and apoptosis in transformed cells. We found that the nucleocytoplasmic shuttling activity is critical for efficient APC1 association and induction of apoptosis in transformed cells. Interestingly, both Apoptin multimerization and APC1 interaction are mediated by domains that overlap with the NES and NLS sequences, respectively. Apoptin expression in transformed cells induces the formation of PML nuclear bodies and recruits APC/C to these subnuclear structures. Our results reveal a mechanism for the selective killing of transformed cells by Apoptin. (+info)
Mutation of the Apc1 homologue shattered disrupts normal eye development by disrupting G1 cell cycle arrest and progression through mitosis. (7/17)The shattered1 (shtd1) mutation disrupts Drosophila compound eye structure. In this report, we show that the shtd1 eye defects are due to a failure to establish and maintain G1 arrest in the morphogenetic furrow (MF) and a defect in progression through mitosis. The observed cell cycle defects were correlated with an accumulation of cyclin A (CycA) and String (Stg) proteins near the MF. Interestingly, the failure to maintain G1 arrest in the MF led to the specification of R8 photoreceptor cells that undergo mitosis, generating R8 doublets in shtd1 mutant eye discs. We demonstrate that shtd encodes Apc1, the largest subunit of the anaphase-promoting complex/cyclosome (APC/C). Furthermore, we show that reducing the dosage of either CycA or stg suppressed the shtd1 phenotype. While reducing the dosage of CycA is more effective in suppressing the premature S phase entry in the MF, reducing the dosage of stg is more effective in suppressing the progression through mitosis defect. These results indicate the importance of not only G1 arrest in the MF but also appropriate progression through mitosis for normal eye development during photoreceptor differentiation. (+info)
Accumulation of substrates of the anaphase-promoting complex (APC) during human cytomegalovirus infection is associated with the phosphorylation of Cdh1 and the dissociation and relocalization of APC subunits. (8/17)Cell cycle dysregulation upon human cytomegalovirus (HCMV) infection of human fibroblasts is associated with the inactivation of the anaphase-promoting complex (APC), a multisubunit E3 ubiquitin ligase, and accumulation of its substrates. Here, we have further elucidated the mechanism(s) by which HCMV-induced inactivation of the APC occurs. Our results show that Cdh1 accumulates in a phosphorylated form that may prevent its association with and activation of the APC. The accumulation of Cdh1, but not its phosphorylation, appears to be cyclin-dependent kinase dependent. The lack of an association of exogenously added Cdh1 with the APC from infected cells indicates that the core APC also may be impaired. This is further supported by an examination of the localization and composition of the APC. Coimmunoprecipitation studies show that both Cdh1 and the subunit APC1 become dissociated from the complex. In addition, immunofluorescence analysis demonstrates that as the infection progresses, several subunits redistribute to the cytoplasm, while APC1 remains nuclear. Dissociation of the core complex itself would account for not only the observed inactivity but also its inability to bind to Cdh1. Taken together, these results illustrate that HCMV has adopted multiple mechanisms to inactivate the APC, which underscores its importance for a productive infection. (+info)
EGFR signaling regulates the proliferation of Drosophila adult midgut progenitors<...
TY - JOUR. T1 - EGFR signaling regulates the proliferation of Drosophila adult midgut progenitors. AU - Jiang, Huaqi. AU - Edgar, Bruce A.. PY - 2009/2/1. Y1 - 2009/2/1. N2 - In holometabolous insects, the adult appendages and internal organs form anew from larval progenitor cells during metamorphosis. As described here, the adult Drosophila midgut, including intestinal stem cells (ISCs), develops from adult midgut progenitor cells (AMPs) that proliferate during larval development in two phases. Dividing AMPs first disperse, but later proliferate within distinct islands, forming large cell clusters that eventually fuse during metamorphosis to make the adult midgut epithelium. We find that signaling through the EGFR/RAS/ MAPK pathway is necessary and limiting for AMP proliferation. Midgut visceral muscle produces a weak EGFR ligand, Vein, which is required for early AMP proliferation. Two stronger EGFR ligands, Spitz and Keren, are expressed by the AMPs themselves and provide an additional, ...
Heterokaryon Technique for Analysis of Cell Type-specific Localization | Protocol
A flexible and efficient method for the characterization of cell type-specific protein localization and nucleocytoplasmic shuttling is...
Mitosis in Neurons: Roughex and APC/C Maintain Cell Cycle Exit to Prevent Cytokinetic and Axonal Defects in Drosophila...
TY - JOUR. T1 - Mitosis in Neurons. T2 - Roughex and APC/C Maintain Cell Cycle Exit to Prevent Cytokinetic and Axonal Defects in Drosophila Photoreceptor Neurons. AU - Ruggiero, Robert. AU - Kale, Abhijit. AU - Thomas, Barbara. AU - Baker, Nicholas E.. PY - 2012/11/1. Y1 - 2012/11/1. N2 - The mechanisms of cell cycle exit by neurons remain poorly understood. Through genetic and developmental analysis of Drosophila eye development, we found that the cyclin-dependent kinase-inhibitor Roughex maintains G1 cell cycle exit during differentiation of the R8 class of photoreceptor neurons. The roughex mutant neurons re-enter the mitotic cell cycle and progress without executing cytokinesis, unlike non-neuronal cells in the roughex mutant that perform complete cell divisions. After mitosis, the binucleated R8 neurons usually transport one daughter nucleus away from the cell body into the developing axon towards the brain in a kinesin-dependent manner resembling anterograde axonal trafficking. Similar ...
Inserm - Optimal T Cell Activation by Melanoma Cells Depends on a Minimal Level of Antigen Transcription
We reported previously that a large fraction of melanoma cell lines induced a suboptimal activation of specific CTL clones, characterized by good tumor cell lysis but no detectable IL-2 production. Using synthetic peptides, we demonstrated recently that this was due to expression of subthreshold levels of appropriate MHC-peptide complexes. We measure here by semiquan-titative reverse transcription-PCR the expression of two melanoma Ag (NA17-A and Melan-A/MART-I) mRNAs in 13 melanoma cell lines and analyze the responses to these cell lines of specific HLA-A2-restricted CTL clones. In line with the idea that the density of MHC-antigenic peptide complexes on melanoma cells is a direct function of the Ags mRNA level, we found that CTL lysis was grossly proportional to this level. We also established that a minimal level of transcription is required for melanoma cells to induce IL-2 secretion. Interestingly, all cell lines that expressed the Ag above this minimal level, either spontaneously or after gene
Study cell division with a complete set of reagents for detecting checkpoint regulators, DNA synthesis, and cell proliferation.
Study cell division with a complete set of reagents for detecting checkpoint regulators, DNA synthesis, and cell proliferation.
The studies presented are the first to describe the structure, function, and expression of human VISTA, a novel, hematopoietically expressed negative checkpoint regulator. Structurally, VISTA is a novel PD-L1-like ligand, with only one IgV domain and whose structure still is not fully resolved. Studies with a newly produced anti-human VISTA mAbs show that human VISTA is highly expressed on myeloid cells with reduced expression on CD4+ and CD8+ T cells. Functionally, human VISTA-Ig is profoundly suppressive on both resting and activated human CD4+ and CD8+ T cells. We propose VISTA as a promising new target for cancer immunotherapy, either as a single target or in combination with other immunotherapeutic strategies.. VISTA has an interesting expression pattern, with greatest mRNA detected in either hematopoietic tissues (i.e., spleen, lymph nodes, and peripheral blood) or those tissues with significant infiltration by leukocytes (Fig. 1). This suggests that VISTA is likely to have important ...
Paras Khadka slams first-ever T20I century for Nepal - Sportstar
Paras Khadka took 49 balls to complete the hundred in Nepals nine-wicket win, also making it the fourth-fastest T20I century by an Asian batsman.
Birkbeck Institutional Research Online
The structure of bacteriophage SPP1 capsid was determined at sub-nanometer resolution by cryo-electron microscopy and single-particle analysis. The icosahedral capsid is composed of the major capsid protein gp13 and the auxiliary protein gp12 that are organized in a T=7 lattice. DNA is arranged in layers with a distance of ∼24.5 Å. Gp12 forms spikes that are anchored at the centre of gp13 hexamers. In a gp12-deficient mutant the centres of hexamers are closed by loops of gp13 coming together to protect the SPP1 genome from the outside environment. The HK97-like fold was used to build a pseudo-atomic model of gp13. Its structural organization remains unchanged upon tail binding and following DNA release. Gp13 exhibits enhanced thermostability in the DNA-filled capsid. A remarkable convergence between thermostability of the capsid and of the other virion components was found, revealing that the overall architecture of the SPP1 infectious particle co-evolved towards high robustness.. ...
APC1 / ANAPC1 antibody | acris-antibodies.com
Kit Component:- KN301218G1, Anapc1 gRNA vector 1 in pCas-Guide vector- KN301218G2, Anapc1 gRNA vector 2 in pCas-Guide vector- KN301218D, donor vector…
ANAPC1 polyclonal antibody - (PAB19231) - Products - Abnova
Rabbit polyclonal antibody rasied against synthetic peptide of ANAPC1. A synthetic peptide corresponding to 17 amino acids near C-terminus of human ANAPC1. (PAB19231) - Products - Abnova
ANAPC4 MaxPab mouse polyclonal antibody (B01) - (H00029945-B01) - Products - Abnova
Mouse polyclonal antibody raised against a full-length human ANAPC4 protein. ANAPC4 (NP_037499, 1 a.a. ~ 808 a.a) full-length human protein. (H00029945-B01) - Products - Abnova
ANAPC16 Gene - GeneCards | APC16 Protein | APC16 Antibody
Complete information for ANAPC16 gene (Protein Coding), Anaphase Promoting Complex Subunit 16, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Gentaur Molecular :ARP \ Antigens ANAPC13, 1-74aa� , Human, T7 tag, E.coli, Recombinant \ 01-P1409
Gentaur molecular products has all kinds of products like :search , ARP \ Antigens ANAPC13, 1-74aa� , Human, T7 tag, E.coli, Recombinant \ 01-P1409 for more molecular products just contact us
Aphasia - condition often leaves people speechless - News - Examiner - Independence, MO
Its been compared to being in a foreign country and not knowing the language or spending 24 hours a day with a word on the tip of your tongue. Imagine hearing and understanding everyone around you, trying to speak, but being unable to form the words. Thats what aphasia sufferers deal with every single day.Frustrating, right? Whats also frustrating is the minimal level of awareness of the condition. Even with 2 million people affected - more people than
Team:Calgary/Parts/Characterization - 2010.igem.org
Figure 2 and 3 indicate the RFP output normalized with growth ratio (OD) at different levels of arabinose. Figure 1 shows that CpxR-I13507 is activated at the highest level when MalE31, the periplasmic misfolder, is expressed. This occurs around 0.2% arabinose concentration. Similar trends are observed in the case of MalE which is a periplasmic folder. MalE and MalE31 activate the system at different levels. MalE31 has similar trends to MalE but has a higher level of RFP expression. These results prove that MalE and MalE31 can both activate the CpxR system however, MalE31, which misfolds, activates it more rapidly and at a lower level of arabinose concentration compared to MalE. If the line of best fit is studied, it is seen that MalE has very minimal level of Cpx activation. Whereas, malE31 has a linear regression which flattens out as the system reaches its upper threshold of detection. Biologically, this could mean that the MalE31 is activated at levels that saturate the cellular chaperones ...
ANAPC7 - DECIPHER v10.3
DECIPHER helps the clinical community share and compare human genome variants and phenotypes in a database of tens of thousands of patients worldwide
Mms19 | Development
Summary: Interaction studies demonstrate that Mms19 forms complexes with Xpd, thereby preventing Xpd-mediated repression of the mitotic kinase activity of the CAK complex and facilitating progression through mitosis. ...
EMDB-1710: Cryo-EM 3D model of the icosahedral particle composed of Rous sar... - Yorodumi
In a mature and infectious retroviral particle, the capsid protein (CA) forms a shell surrounding the genomic RNA and the replicative machinery of the virus. The irregular nature of this capsid shell precludes direct atomic resolution structural analysis. CA hexamers and pentamers are the fundamental building blocks of the capsid, however the pentameric state, in particular, remains poorly characterized. We have developed an efficient in vitro protocol for studying the assembly of Rous sarcoma virus (RSV) CA that involves mild acidification and produces structures modeling the authentic viral capsid. These structures include regular spherical particles with T = 1 icosahedral symmetry, built from CA pentamers alone. These particles were subject to cryoelectron microscopy (cryo-EM) and image processing, and a pseudo-atomic model of the icosahedron was created by docking atomic structures of the constituent CA domains into the cryo-EM-derived three-dimensional density map. The N-terminal domain ...
Diamond Publications - Search Results
Abstract: Cell entry of enveloped viruses requires specialized viral proteins that mediate fusion with the host membrane by substantial structural rearrangements from a metastable pre- to a stable postfusion conformation. This metastability renders the herpes simplex virus 1 (HSV-1) fusion glycoprotein B (gB) highly unstable such that it readily converts into the postfusion form, thereby precluding structural elucidation of the pharmacologically relevant prefusion conformation. By identification of conserved sequence signatures and molecular dynamics simulations, we devised a mutation that stabilized this form. Functionally locking gB allowed the structural determination of its membrane-embedded prefusion conformation at sub-nanometer resolution and enabled the unambiguous fit of all ectodomains. The resulting pseudo-atomic model reveals a notable conservation of conformational domain rearrangements during fusion between HSV-1 gB and the vesicular stomatitis virus glycoprotein G, despite their ...
ANTIGEN PRESENTATION AND ASSEMBLY BY MOUSE I-A(K) CLASS-II MOLECULES IN HUMAN APC CONTAINING DELETED OR MUTATED HLA DM GENES -...
ANTIGEN PRESENTATION AND ASSEMBLY BY MOUSE I-A(K) CLASS-II MOLECULES IN HUMAN APC CONTAINING DELETED OR MUTATED HLA DM GENES Journal Articles ...
Light Exposure During Pregnancy Key to Normal Eye Development | UC San Francisco
New research in Nature concludes the eye - which depends on light to see - also needs light to develop normally during pregnancy.
ANAPC1 Gene - GeneCards | APC1 Protein | APC1 Antibody
Complete information for ANAPC1 gene (Protein Coding), Anaphase Promoting Complex Subunit 1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Anaphase Promoting Complex Subunit 13 (ANAPC13) Antikörper
Reaktivität: Human, Maus, Ratte. 21 verschiedene ANAPC13 Antikörper vergleichen. Alle direkt auf antikörper-online bestellbar!
Tissue expression of ANAPC4 - Staining in soft tissue 1 - The Human Protein Atlas
Expression of ANAPC4 (APC4) in soft tissue 1 tissue. Antibody staining with HPA038395, HPA038396 and CAB032519 in immunohistochemistry.
Granted, if you are simply looking for a better job, during your job search, while still being gainfully employed the level of stress may not be as significant because the job search is voluntary. But if youre looking for that dream job and your job search has come to a pleasant result and you are scheduled for an interview you will still be experiencing some stress and perhaps more stress than usual under the circumstances because it is after all your dream job which was the result of your job search. So what can you do to better manage the stress about the potential of being unemployed or looking for a new job with the forced job search? Be prepared for what the new potential job interviewer may ask wont generate much stress because this can be done through surfing on the net and should be part of your job search. Also, doing research about the management style or culture for the job in mind can also result in minimal levels of stress during the job search. Then we finalize the resume, make ...
Pooch plunge is the one weekend each year that our Gorman Outdoor Pool is available for a dog swim during Newbarket before the pool closes down for the winter. It is a shallow wading entry pool with a small deep end for those dogs that like to jump in. The pool enclosure is an off-leash area and is fully fenced in. The chlorine levels are kept at the minimal level required for public health. There will be a hose available on the pool deck to rinse your dog BEFORE and AFTER going in the water ...
Axonopathy and Transport Deficits Early in the Pathogenesis of Alzheimers Disease | Science
We identified axonal defects in mouse models of Alzheimers disease that preceded known disease-related pathology by more than a year; we observed similar axonal defects in the early stages of Alzheimers disease in humans. Axonal defects consisted of swellings that accumulated abnormal amounts of microtubule-associated and molecular motor proteins, organelles, and vesicles. Impairing axonal transport by reducing the dosage of a kinesin molecular motor protein enhanced the frequency of axonal defects and increased amyloid-β peptide levels and amyloid deposition. Reductions in microtubule-dependent transport may stimulate proteolytic processing of β-amyloid precursor protein, resulting in the development of senile plaques and Alzheimers disease. ...
Anti ANAPC7 Antibody (PrecisionAb Monoclonal Antibody) | Bio-Rad
Anaphase-promoting complex subunit 7 antibody to detect human Anaphase-promoting complex subunit 7. Validated for western blotting. Try a trial size today.
Hamas and Israel on Verge of the Deal - Paperblog
I still consider a two-state solution be possible. The final status agreement has been very close at least since Beilin-Abu Mazen understandings/agreement/plan (1995) where nearly all issues were agreed. The Olmert proposal (2008) was probably the last serious try (both plans can be found from my document library ) If however it cant be negotiated so there is possibilities to make regional solution; I for example have long propagated the idea of the Three-State-(return) Option ( e.g. in The Three-State Option could solve Gaza Conflict ). Also - if two-state solution is de facto cul-de-sac and if there is no readiness to regional solution so a unilateral Cold Peace solution from my perspective is the best option especially if Hamas-Israel Deal will come true.. Israel could independently implement a Cold Peace Solution, a minimal level of peace relations, to ensure its character as a Jewish and democratic state, by fixing a border between Israel and a future Palestinian state in the West ...
Warshaw Collection of Business Americana Subject Categories: Cookbooks | Collection | SOVA
In 2016, with funding provided by the Smithsonian Institutions Collections Care and Preservation Fund, the Archives Center at the National Museum of American History implemented the use of minimal level processing standards to increase information about and facilitate access to more of our collections. A large portion of stored material from the original acquisition received minimal level processing, which resulted in additions to this Subject category. This effort included basic arrangement and replacement of non-archival housing for long-term stability, but staples and other fasteners have not all been removed. Revisions to the encoded finding aid were made to reflect the added content in context to the previously processed material ...
Tissue expression of ANAPC4 - Staining in lymph node - The Human Protein Atlas
Expression of ANAPC4 (APC4) in lymph node tissue. Antibody staining with HPA038395, HPA038396 and CAB032519 in immunohistochemistry.
ANAPC13 - PCR Primer Pair - SYBR | PrimePCR | Bio-Rad
Perform reliable qPCR with Bio-Rads pre-validated ANAPC13 primer pair, for the Human genome. Designed for SYBR Green-based detection.
Patent US4614750 - Ruthenium-copper-containing, activated-carbon-supported catalyst and process ... - Google Patents
A catalyst is disclosed which comprises a ruthenium-copper-containing complex of the formula M.sub.a A.sub.b RuCu.sub.c N.sub.z O.sub.x wherein M is selected from the group consisting of Ce, Cr, Fe, Mn, and mixtures thereof, A is an alkali metal, alkaline earth metal or mixture thereof, a is from 0 to about 1, b is from about 0.002 to about 10, c is from about 0.2 to about 20, z is from 0 to about 1% by weight, x is the number of oxygens needed to fulfill the valence requirements of the other elements, said complex being supported by an activated carbon support having a surface area of at least about 300 square meters per gram. A process for converting mixtures of hydrogen and carbon monoxide, particularly synthesis gas, to alcohol with minimal levels of hydrocarbon by-products being formed is also disclosed.
Blood donors needed following marathon bombing - Newsworthy
The blood donated locally with LifeShare will be available to help replenish the blood supply in Boston after our local needs are met. Being a community blood center, we must continue to be prepared for the blood requirements for medical needs in our community. This month, the local blood supply has been at a minimal level. In order to support the needs around the Boston area, we need additional blood donors and donations to ensure the local supply doesnt fall to a critical level, says Tina Hooper, spokesperson for LifeShare Blood Centers ...
Prostate cancers develop immunosuppressive microenvironment resisting current treatments and the emerging immunotherapies. Hossain and colleagues identified a population of Lin−CD15HICD33LO granulocytic MDSCs (G-MDSCs) accumulating in patients during prostate cancer progression. The G-MDSCs secreted large amounts of Arginase 1, thereby suppressing T cells proliferation and activity. The MDSC functions were under control of the STAT3 transcription factor and a central immune checkpoint regulator. To functionally eliminate prostate cancer-associated G-MDSC, the authors successfully employed an siRNA-based approach to silence STAT3 specifically in TLR9-expressing G-MDSCs. These findings underscore the potential of oligonucleotide-based therapeutics to alleviate immunosuppressive signaling in advanced prostate cancers. ...
A Note of Growth and Inequality in Peru, 2003-2008
Downloadable! This note reports information on the income inequality in Peru calculated from Income Household surveys from 2003-2008. Using surveys from the ENAHO published by the National Institute of Statistics, we used as index the household income annualized, it was divided by the total members of each household to compute the inequality indicators. We computed the density of income distribution using nonparametric methods (Kernel) then we used bootstrapping techniques to check the statistic significance of the inequality indexes variation using the K-S and the MWM to test the null hypothesis of no changes in income inequality between the periods. We conclude that the changes in the inequality indexes indeed have been reducing but in very minimal level even though the economic activity (real GDP) grew at sustained rates, 7.3% in average.
Moving Beyond the Exercise: Exercise Prescription and Clinical Reasoning | Physio Praxis
The preceding paragraph was intended to point out specific examples where the intervention wasnt matched to the demand. In many cases, such as postoperative quad weakness, strength does play a significant role. In these situations, its important that the dosage is appropriate in order to elicit the adaptations needed while avoiding negative responses. This minimum effective dose was defined by Siff in Supertraining as the condition which optimally promotes hypertrophy and strength increase [via] the production of some minimal level of force for some minimal amount of time.10 Unless intensity (defined as a percentage of rep max) is considered, even the overly popular 3 sets of 10 is a meaningless prescription. Delorme used progressive loading in his protocol reaching a true 10 repetition maximum on the third set.11 It appears that taking at least one set to failure is very important for eliciting adaptations, and this appears to hold true even with significant deviation from the commonly ...
Dietary Impact on Neuronal Autophagy Control and Brain Health | IntechOpen
Autophagy is the major intracellular system which is critical for the removal of harmful protein aggregates and malfunctioning organelles. Dysfunctional autophagy is associated with a multitude of human diseases, such as protein aggregation in Alzheimers disease and non-successful aging. Major interest exists in the dietary manipulation of the autophagy pathway activity, so as to tune the cells protein degradation capabilities and to prevent cell death onset. It has recently become clear that the machinery required to degrade protein cargo has a distinct activity level which can be altered through specific dietary modulation. Moreover, this activity may differ from that of the proteinaceous cargo. Overall, brain health and successful aging are characterized by limited protein aggregation, with a distinct molecular signature of maintained autophagy function. However, it is largely unclear how to control autophagy through dietary interventions with a precision that would allow to maintain minimal levels
An important person in the defensin family, in rat lungs. mold. | Identification of genotype-correlated sensitivity to...
An important person in the defensin family, in rat lungs. mold. It really is mainly expressed in a number of mucosa epithelial cells of your skin, bronchus 681806-46-2 manufacture and urogenital system . Its exclusive sterilizing mechanism would be to damage undamaged bacterial lipid membranes, in order to modification the permeability of mobile membranes. Therefore, it isnt an easy task to induce level of resistance in bacteria and may be used like a potential fresh antibiotic, rendering it a popular subject in anti-infection research . Under regular circumstances, is indicated hardly ever or at a minimal level, and it is up-regulated to operate in cells of varied mucosa tissues subjected to stimuli, including proinflammatory cytokines and microorganisms. Different microorganism parts can induce manifestation of different defensins [3,4]. Furthermore, like a signaling molecule, may also induce and recruit inflammatory and immune system cells, and impact mobile proliferation and ...
Anaphase-promoting complex, subunit CDC26 (IPR018860) | InterPro | EMBL-EBI
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
Anaphase-promoting complex subunit 1 (IPR024990) | InterPro | EMBL-EBI
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
RCSB PDB - 5L9U: Model of human Anaphase-promoting complex/Cyclosome (APC/C-CDH1) with a cross linked Ubiquitin variant...
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Hyperlipid: 09/01/2013 - 10/01/2013
Final thought: Do we need omega 3 PUFA at anything above the most minimal levels if we are in saturated fat based ketosis? Of course I dont know. But the signal to cope with starvation is palmitic acid (physiological insulin resistance), not DHA. I live in starvation mode, not on a mixed diet with only intermittent access to healthy ruminant fat. I have long wanted to look at the selective release of FFAs from adipocytes in extended starvation. My suspicion is that in the early days after glycogen depletion palmitic acid is preferentially released over other lipids, PUFA are not needed/wanted. By a few weeks all the palmitate is gone and whatever is left then gets released. People like David Blaine suddenly start to feel weak, wobbly and are probably hypoglycaemic once they run out of palmitate and have to release less saturated fats. Two to four weeks if you carry some spare weight. Sauers rats had only ever been fed a low fat omega 6 based diet and had no serious palmitate reserves, PUFA ...
The anaphase-promoting complex/cyclosome › Research Explorer
BACKGROUND: The aggressive B-cell non-Hodgkin lymphomas diffuse large B-cell lymphoma (DLBCL) and mantle cell lymphoma (MCL) are characterised by a high proliferation rate. The anaphase-promoting complex/cyclosome (APC/C) and its co-activators Cdc20 and Cdh1 represent an important checkpoint in mitosis. Here, the role of the APC/C and its co-activators is examined in DLBCL and MCL.. METHODS: The expression and prognostic value of Cdc20 and Cdh1 was investigated using GEP data and immunohistochemistry. Moreover, the therapeutic potential of APC/C targeting was evaluated using the small-molecule inhibitor proTAME and the underlying mechanisms of action were investigated by western blot.. RESULTS: We demonstrated that Cdc20 is highly expressed in DLBCL and aggressive MCL, correlating with a poor prognosis in DLBCL. ProTAME induced a prolonged metaphase, resulting in accumulation of the APC/C-Cdc20 substrate cyclin B1, inactivation/degradation of Bcl-2 and Bcl-xL and caspase-dependent apoptosis. In ...
NMN deamidase delays Wallerian degeneration and rescues axonal defects caused by NMNAT2 deficiency in vivo - Nottingham ePrints
Axons require the axonal NAD-synthesizing enzyme NMNAT2 to survive. Injury or genetically-induced depletion of NMNAT2 triggers axonal degeneration or defective axon growth. We have previously proposed that axonal NMNAT2 primarily promotes axon survival by maintaining low levels of its substrate NMN, rather than generating NAD; however, this is still debated. NMN deamidase, a bacterial enzyme, shares NMN-consuming activity with NMNAT2, but not NAD-synthesizing activity, and delays axon degeneration in primary neuronal cultures. Here, we show that NMN deamidase can also delay axon degeneration in zebrafish larvae and in transgenic mice. Like overexpressed NMNATs, NMN deamidase reduces NMN accumulation in injured mouse sciatic nerves and preserves some axons for up to 3 weeks, even when expressed at a low level. Remarkably, NMN deamidase also rescues axonal outgrowth and perinatal lethality in mice lacking NMNAT2 in a dose-dependent manner. These data further support a pro-degenerative effect of ...
Nanosecond laser may be safe for cataract surgery at a lower cost than femtosecond laser
COPENHAGEN — Nanosecond laser may represent an affordable, safe and effective way of doing laser cataract surgery, according to one speaker.“A minimal level of energy is applied to the eye. There is no thermal alteration in the anterior chamber, no mechanical or thermal side effects,” Jérôme C. Vryghem, MD, said at the European Society of Cataract and Refractive Surgeons
Tip of the week: Use caution when assigning HCPCS skin substitute codes - www.hcpro.com
Be careful when assigning HCPCS skin substitute codes; the number of units should be equal to the number of square centimeters to which the substitute applied. Also check the skin substitute application CPT code descriptions. The number of units is not the same for all skin substitute application codes, said Kathleen D. Schaum, MS, president of Kathleen D. Schaum & Associates, Inc., in Lake Worth, FL. Be sure the physicians document these procedures completely, including his or her choice of fixation. Your Medicare contractor might specify a minimal level of fixation.
APC2 - Adenomatous polyposis coli protein 2 - Homo sapiens (Human) - APC2 gene & protein
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
MojoSort Human anti-APC Nanobeads - APC (Allophycocyanin)
|p|Human APC labeled PBMCs are either selected or depleted in a two-step separation process. The cells are first incubated with a BioLegend anti-human APC-conjugated antibody, followed by incubating with directly conjugated anti-APC Nanobeads. The APC|sup|+|/sup|, magnetically labeled fraction is re
The Chamber was honored to host a ribbon cutting for APC Roofing which is located at 150 W. Highway 50 in Clermont. APC Roofing is fully licensed and insured to handle any size project. They are locally owned and operated and have decades of experience. You can learn more about APC Roofing on their website…. ...
APC提供的産品支持 - APC Taiwan, China
Vodermaier HC, Gieffers C, Maurer-Stroh S, Eisenhaber F, Peters JM (Sep 2003). "TPR subunits of the anaphase-promoting complex ... "Mammalian p55CDC mediates association of the spindle checkpoint protein Mad2 with the cyclosome/anaphase-promoting complex, and ... Jörgensen PM, Gräslund S, Betz R, Ståhl S, Larsson C, Höög C (Jan 2001). "Characterisation of the human APC1, the largest ... "TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1". Current Biology. 13 (17): 1459- ...
These include Apc1, the largest subunit which contains 11 tandem repeats of 35-40 amino acid sequences, and Apc2, which ... April 2015). "Structure of an APC3-APC16 complex: insights into assembly of the anaphase-promoting complex/cyclosome". Journal ... Anaphase-promoting complex (also called the cyclosome or APC/C) is an E3 ubiquitin ligase that marks target cell cycle proteins ... a Drosophila gene encoding the Cdc27 subunit of the anaphase promoting complex, enhance centrosomal defects in polo and are ...
ANAPC1 Gene - GeneCards | APC1 Protein | APC1 Antibody
Anaphase Promoting Complex Subunit 1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards ... Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls ... Q9H1A4-APC1_HUMAN. Recommended name:. Anaphase-promoting complex subunit 1 Protein Accession:. Q9H1A4. Secondary Accessions: * ... Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. (PMID: 11179667) Jörgensen PM … Höög ...
Recombinant Human Apc1 protein (ab163855) | Abcam
Buy our Recombinant Human Apc1 protein. Ab163855 is a protein fragment produced in Wheat germ and has been validated in WB, ... Anaphase-promoting complex subunit 1. *Apc 1. *APC1. *APC1_HUMAN. *Cyclosome subunit 1 ... Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls ... Anaphase promoting complex subunit 1. *anaphase-promoting complex 1 (meiotic checkpoint regulator) ...
APC1 / ANAPC1 antibody | acris-antibodies.com
Alternative names for APC1 / ANAPC1 antibody. Anaphase-promoting complex subunit 1, TSG24, MCPR, Cyclosome subunit 1, Testis- ... Western blot analysis of APC1 in SK-N-SH cell lysate with APC1 antibody at (A) 1 and (B) 2 ug/mL.. *TA306967 ... WB using Anti-APC1 pS377 antibody shows detection of a band ~215 kDa corresponding to phosphorylated human APC1 (arrowhead). ... WB using Anti-APC1 pS355 antibody shows detection of a band ~215 kDa corresponding to phosphorylated human APC1 (arrowhead). ...
Apc1 Peptides and Proteins: Novus Biologicals
Browse our Apc1 Peptides and Proteins all backed by our Guarantee+. ... Apc1 Peptides and Proteins available through Novus Biologicals. ... anaphase promoting complex subunit 1 protein, anaphase- ... anaphase-promoting complex subunit 1 protein, APC1Testis-specific gene 24 protein protein, Cyclosome subunit 1 protein, Mitotic ... Our Apc1 Peptides and Apc1 Proteins can be used in a variety of model species: Human. Use the list below to choose the Apc1 ...
The Schizosaccharomyces pombe spindle checkpoint protein mad2p blocks anaphase and genetically interacts with the anaphase...
... also called the cyclosome or anaphase promoting complex (APC) (15-17). APC catalyzes the transfer of ubiquitin from ubiquitin- ... Four of the subunits have also been identified in S. cerevisiae: CDC16, CDC23, CDC27, and APC1 (19). Fission yeast cut9, nuc2, ... One possible target of the spindle checkpoint is anaphase promoting complex (APC), which controls all postmetaphase events that ... pombe spindle checkpoint protein mad2p blocks anaphase and genetically interacts with the anaphase-promoting complex. Xiangwei ...
RNA/DNA Purification Micro Kit (Cat. 50300) | Norgen Biotek Corp.
... of Human Cytomegalovirus UL21a and UL97 to Viral Growth and Inactivation of the Anaphase Promoting Complex/Cyclosome (APC/C) E3 ... Ubiquitin Ligase Reveal a Unique Cellular Mechanism for Down-modulation of the APC/C Subunits APC1, APC4, and APC5.. ... Nicotine promotes apoptosis resistance of breast cancer cells and enrichment of side population cells with cancer stem cell- ...
anapc1, anaphase promoting complex subunit 1 - Creative Biogene
ANAPC1; anaphase promoting complex subunit 1; anaphase-promoting complex subunit 1; APC1; MCPR; TSG24; cyclosome subunit 1; ... ANAPC1 is 1 of at least 10 subunits of the anaphase-promoting complex (APC), which functions at the metaphase-to-anaphase ... mitotic checkpoint regulator; testis-specific gene 24 protein; anaphase-promoting complex 1 (meiotic checkpoint regulator) ...
Apc1 Subunit, Anaphase Promoting Complex Apc1 Subunit, Anaphase Promoting Complex Cyclosome Apc1 Subunit, Anaphase-Promoting ... Apc1 Subunit, Anaphase Promoting Complex. Apc1 Subunit, Anaphase Promoting Complex Cyclosome. Apc1 Subunit, Anaphase-Promoting ... Apc1 Subunit, Anaphase Promoting Complex Cyclosome [D08.811.464.938.750.092.500] Apc1 Subunit, Anaphase Promoting Complex ... Apc10 Subunit, Anaphase Promoting Complex Cyclosome [D08.811.464.938.750.092.625] Apc10 Subunit, Anaphase Promoting Complex ...
C-terminal region of apoptin affects chicken anemia virus replication and virulence | Virology Journal | Full Text
... and APC-1, a subunit of the anaphase-promoting complex/cyclosome, which is involved in the assembly and regulation of the ... The viral protein Apoptin associates with the anaphase-promoting complex to induce G2/M arrest and apoptosis in the absence of ... APC-1:. Subunit of the anaphase-promoting complex/cyclosome. CAV:. Chicken anemia virus ... cyclosome complex . VP3 has also been reported to interact with the cytoplasmic protein HIPPI, a protein that interacts ...
Identification of Subunits of the Anaphase-Promoting Complex of Saccharomyces cerevisiae | Science
This particle, called the anaphase-promoting complex (APC) or cyclosome, functions as a cell cycle-regulated ubiquitin-protein ... Two additional subunits of the budding yeast APC were identified: The largest subunit, encoded by the APC1 gene, is conserved ... Identification of Subunits of the Anaphase-Promoting Complex of Saccharomyces cerevisiae. By Wolfgang Zachariae, Tae Ho Shin, ... Identification of Subunits of the Anaphase-Promoting Complex of Saccharomyces cerevisiae. By Wolfgang Zachariae, Tae Ho Shin, ...
Interactive Fly, Drosophila
The anaphase-promoting complex or cyclosome (APC) is an unusually complicated ubiquitin ligase, composed of 13 core subunits ... The cullin subunit APC2 and its binding partner, the RING finger protein APC11, are found in a subcomplex with APC1, APC4, and ... Microinjection of antibodies against subunits of the anaphase-promoting complex/cyclosome or against human Cdc20 (fizzy) ... one of the substrate-targeting subunits of the anaphase-promoting complex (APC). However, Cdh1, another targeting subunit used ...
Co-regulation proteomics reveals substrates and mechanisms of APC/C-dependent degradation. - Semantic Scholar
... we clustered the proteins with abundance profiles most similar to known Anaphase-Promoting Complex/Cyclosome (APC/C) substrates ... Apc1 Subunit, Anaphase-Promoting Complex-Cyclosome. *Cell (microprocessor). *Motif. *KIF18A gene. Citations. Publications ... we clustered the proteins with abundance profiles most similar to known Anaphase-Promoting Complex/Cyclosome (APC/C) substrates ... SUMO targets the APC/C to regulate transition from metaphase to anaphase. *Karolin Eifler, Sabine A. G. Cuijpers, +5 authors ...
High Affinity and Specificity APC1 Polyclonal Antibody at Elabscience.com
Our APC1 Polyclonal Antibody price is reasonable. Check more details about APC1 Polyclonal Antibody now. ... anaphase-promoting complex 1 (meiotic checkpoint regulator),Anaphase-promoting complex subunit 1,Apc 1,APC1,APC1,Cyclosome ... anaphase-promoting complex 1 (meiotic checkpoint regulator),Anaphase-promoting complex subunit 1,Apc 1,APC1,APC1,Cyclosome ... This gene encodes a subunit of the anaphase-promoting complex. This complex is an E3 ubiquitin ligase that regulates ...
Institute of Cancer Research Repository - WD40 domain of Apc1 is critical for the coactivator-induced allosteric transition...
The anaphase-promoting complex/cyclosome (APC/C) is a large multimeric cullin-RING E3 ubiquitin ligase that orchestrates cell- ... The platform comprises APC/C subunits Apc1, Apc4, Apc5, and Apc15. Although the role of Apc1 as an APC/C scaffolding subunit ... APC/C ubiquitination cell cycle UbcH10 Ube2S ANAPHASE-PROMOTING COMPLEX EM STRUCTURE DETERMINATION UBIQUITIN-CHAIN FORMATION ... Apc1(WD40)). To understand how Apc1(WD40) contributes to APC/C activity, a mutant form of the APC/C with Apc1(WD40) deleted was ...
Anti-APC10(A95-363R) - SignalChem Online Store
2. Jorgensen PM et al: Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. Gene2001; 262 ... 1. Peters JM: The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol.2006; 7:644-56 ... 3. Passmore LA et al: Doc1 mediates the activity of the anaphase-promoting complex by contributing to substrate recognition. ...
Table 1: List of all of the K
prt okam11e05d AJ229521 229 okam11e05r AJ229522 149 9.0 E-08 YNL172w APC1 subunit of anaphase-promoting complex (cyclosome) ... to human PL6 prt am2e05d AJ229445 151 am2e07d AJ229446 119 6.7 E-07 YLR292c SEC72 ER prt-translocation complex subunit am2e10d ... III, 160 KD subunit okam5g05r AJ229937 405 1.1 E-96 YOR117w RPT5 26S proteasome subunit okam5g06d AJ229938 193 repeats or mito ... subunit of the RSC complex am2d07d AJ229438 191 am2d08d AJ229439 125 am2d09d AJ229440 358 2.9 E-08 YPR049c simil. to Uso1p ...
Anaphase Promoting Complex Subunit 1 (ANAPC1) Antikörper
... anaphase-promoting complex 1 (meiotic checkpoint regulator) , anaphase-promoting complex subunit 1 , cyclosome subunit 1 , ... cryo-EM structure of an APC (zeige APC Antikörper)/C-Cdh1 (zeige CDH1 Antikörper) complex with Apc1 (zeige SLC25A24 Antikörper ... Weitere Produktkategorien zu Anaphase Promoting Complex Subunit 1 Antikörper * 131 anti-Anaphase Promoting Complex Subunit 1 ... anti-Anaphase Promoting Complex Subunit 1 (ANAPC1) Antikörper. ANAPC1 encodes a subunit of the anaphase-promoting complex. ...
DeCS 2014 - New terms
Apc1 Subunit, Anaphase-Promoting Complex-Cyclosome. Subunidade Apc1 do Ciclossomo-Complexo Promotor de Anáfase. Subunidad Apc1 ... Apc1 Subunit, Anaphase-Promoting Complex-Cyclosome. Subunidade Apc1 do Ciclossomo-Complexo Promotor de Anáfase. Subunidad Apc1 ... Apc2 Subunit, Anaphase-Promoting Complex-Cyclosome. Subunidade Apc2 do Ciclossomo-Complexo Promotor de Anáfase. Subunidad Apc2 ... Apc3 Subunit, Anaphase-Promoting Complex-Cyclosome. Subunidade Apc3 do Ciclossomo-Complexo Promotor de Anáfase. Subunidad Apc3 ...
Sequence variation at ANAPC1 accounts for 24% of the variability in corneal endothelial cell density. - PubMed - NCBI
anaphase-promoting complex (APC): the sum of its parts? | Biochemical Society Transactions | Portland Press
Apc1. With 1748 amino acids, Apc1 is the largest subunit of the APC and, intriguingly, it has no known function. It contains ... The APC (anaphase-promoting complex) or cyclosome is a large multisubunit protein complex. It has 13 core components (with ... The anaphase-promoting complex (APC): the sum of its parts? L.A. Passmore L.A. Passmore ... The APC (anaphase-promoting complex) is a multisubunit E3 ubiquitin ligase that targets cell-cycle-related proteins for ...
Anapc1 MGI Mouse Gene Detail - MGI:103097 - anaphase promoting complex subunit 1
Search Articles | University of Toronto Libraries
Apc1 Subunit, Anaphase-Promoting Complex-Cyclosome - chemistry , Apc8 Subunit, Anaphase-Promoting Complex-Cyclosome - chemistry ... Apc11 Subunit, Anaphase-Promoting Complex-Cyclosome - metabolism , Apc1 Subunit, Anaphase-Promoting Complex-Cyclosome - ... Apc7 Subunit, Anaphase-Promoting Complex-Cyclosome - chemistry , Apc3 Subunit, Anaphase-Promoting Complex-Cyclosome - ... Apc3 Subunit, Anaphase-Promoting Complex-Cyclosome - chemistry , Apc7 Subunit, Anaphase-Promoting Complex-Cyclosome - ...
CDC23 - Anaphase-promoting complex subunit CDC23 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) -...
In early mitosis, the APC/C is activated by CDC20 and targets securin PDS1, the B-type cyclin CLB5, and other anaphase ... a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the ... to allow exit from mitosis and creating the low CDK state necessary for cytokinesis and for reforming prereplicative complexes ... inhibitory proteins for proteolysis, thereby triggering the separation of sister chromatids at the metaphase-to-anaphase ...
Mechanisms controlling the temporal degradation of Nek2A and Kif18A by the APC/C-Cdc20 complex. - PubMed - NCBI
Ubiquitin-Protein Ligase Complexes/physiology*. Substances. *Apc8 Subunit, Anaphase-Promoting Complex-Cyclosome ... anti-APC1 and anti-APC7 antibodies. (G) Relative binding of Myc-Nek2A, APC1 and APC7 to FLAG-Venus-Nek2A WT, Nek2A L313A/L320A ... The Anaphase Promoting Complex/Cyclosome (APC/C) in complex with its co-activator Cdc20 is responsible for targeting proteins ... Nek2A and the mitotic checkpoint complex (MCC) have an overlap in APC/C subunit requirements for binding and we propose that ...
Diminishing HDACs by drugs or mutations promotes normal or abnormal sister chromatid separation by affecting APC/C and adherin ...
The levels of Cut9, an anaphase-promoting complex/cyclosome (APC/C) subunit, however, were not significantly affected in the ... 3A). Cut4/Apc1 is the largest subunit of APC/C and is proposed to bridge two subcomplexes within APC/C, whereas Doc1/Apc10 was ... phenotypes of the mutants of the ubiquitin ligase complex anaphase-promoting complex/cyclosome (APC/C), which display metaphase ... Doc1 mediates the activity of the anaphase-promoting complex by contributing to substrate recognition. EMBO J. 22, 786-796. ...
Different phosphorylation states of the anaphase promoting complex in response to antimitotic drugs: A quantitative proteomic...
1,a compilation of phosphorylation site data for the largest APC subunit, APC1, shows very different patterns of ... 2006) Polo-like kinase 1: Target and regulator of anaphase-promoting complex/cyclosome-dependent proteolysis. Cancer Res 66: ... 2005) Uncoupling anaphase-promoting complex/cyclosome activity from spindle assembly checkpoint control by deregulating polo- ... 1996) Identification of BIME as a subunit of the anaphase-promoting complex. Science 274:1199-1201. ...
Interactive Fly, Drosophila
Anaphase-promoting complex/cyclosome (APC) specifically ubiquitinates Cut2/Pds1 at metaphase-anaphase transition, and ... Furthermore, at least in vitro APC activation can be suppressed by PKA, which phosphorylates two APC subunits, APC1 and APC3. ... The anaphase-promoting complex/cyclosome (APC/C) is a cell-cycle-regulated essential E3 ubiquitin ligase; however, very little ... Proteolysis of Pds1 is mediated by a ubiquitin-protein ligase, the anaphase-promoting complex (APC) or cyclosome. The APC is ...
Hypomorphic bimAAPC3 Alleles Cause Errors in Chromosome Metabolism That Activate the DNA Damage Checkpoint Blocking Cytokinesis...
1998 Regulation of the anaphase-promoting complex/cyclosome by bimAAPC3 and proteolysis of NIMA. Mol. Biol. Cell 9: 3019-3030. ... Similar phenotypes are associated with mutations in the APC/C subunits BIMEAPC1 and BIMHAPC6 (Lieset al. 1998; P. M. Mirabito, ... Here we show that sepI1 is an allele of bimAAPC3, which encodes a component of the anaphase-promoting complex/cyclosome (APC/C ... 1997 MAD2 associates with the cyclosome/anaphase-promoting complex and inhibits its activity. Proc. Natl. Acad. Sci. USA 94: ...
YRC Public Data Repository - Protein Overview - APC9 / YLR102C
Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of ... APC1 APC2 APC4 APC5 APC9 CDC16 CDC23 CDC27 View Details. Qiu et al. (2008) APC1 APC11 APC2 APC4 APC5 APC9 CDC16 CDC23 CDC26 ... anaphase-promoting complex [TAS. ] Biological Process:. mitotic spindle elongation [TAS. ] cyclin catabolic process [TAS. ] ... anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process [IPI. ] mitotic metaphase/ ...
Study of Cyclin Proteolysis in Anaphase-Promoting Complex (APC) Mutant Cells Reveals the Requirement for APC Function in the...
1997) Distinct subunit functions and cell cycle regulated phosphorylation of 20S APC/cyclosome required for anaphase in fission ... In S. pombe, seven components of the APC have been identified to date: Cut4p (APC1) (42). Cut9p (homologous to Saccharomyces ... 1999) The Schizosaccharomyces pombe dim1(+) gene interacts with the anaphase-promoting complex or cyclosome (APC/C) component ... 1999) Whose end is destruction: cell division and the anaphase-promoting complex. Genes Dev. 13:2039-2058. ...