Nucleic acids which hybridize to complementary sequences in other target nucleic acids causing the function of the latter to be affected.
RNA molecules which hybridize to complementary sequences in either RNA or DNA altering the function of the latter. Endogenous antisense RNAs function as regulators of gene expression by a variety of mechanisms. Synthetic antisense RNAs are used to effect the functioning of specific genes for investigative or therapeutic purposes.
Short fragments of DNA or RNA that are used to alter the function of target RNAs or DNAs to which they hybridize.
DNA that is complementary to the sense strand. (The sense strand has the same sequence as the mRNA transcript. The antisense strand is the template for mRNA synthesis.) Synthetic antisense DNAs are used to hybridize to complementary sequences in target RNAs or DNAs to effect the functioning of specific genes for investigative or therapeutic purposes.
Short fragments of DNA that are used to alter the function of target RNAs or DNAs to which they hybridize.
The branch of science concerned with the means and consequences of transmission and generation of the components of biological inheritance. (Stedman, 26th ed)
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
Short fragments of RNA that are used to alter the function of target RNAs or DNAs to which they hybridize.
Nucleotide sequences, usually upstream, which are recognized by specific regulatory transcription factors, thereby causing gene response to various regulatory agents. These elements may be found in both promoter and enhancer regions.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
The discipline studying genetic composition of populations and effects of factors such as GENETIC SELECTION, population size, MUTATION, migration, and GENETIC DRIFT on the frequencies of various GENOTYPES and PHENOTYPES using a variety of GENETIC TECHNIQUES.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
The experimental study of the relationship between the genotype of an organism and its behavior. The scope includes the effects of genes on simple sensory processes to complex organization of the nervous system.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Nucleic acid sequences involved in regulating the expression of genes.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
A group of chemical elements that are needed in minute quantities for the proper growth, development, and physiology of an organism. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A computer based method of simulating or analyzing the behavior of structures or components.
Substances that comprise all matter. Each element is made up of atoms that are identical in number of electrons and protons and in nuclear charge, but may differ in mass or number of neutrons.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
The Alu sequence family (named for the restriction endonuclease cleavage enzyme Alu I) is the most highly repeated interspersed repeat element in humans (over a million copies). It is derived from the 7SL RNA component of the SIGNAL RECOGNITION PARTICLE and contains an RNA polymerase III promoter. Transposition of this element into coding and regulatory regions of genes is responsible for many heritable diseases.
Established cell cultures that have the potential to propagate indefinitely.
Theoretical representations that simulate the behavior or activity of genetic processes or phenomena. They include the use of mathematical equations, computers, and other electronic equipment.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Nucleotides in which the base moiety is substituted with one or more sulfur atoms.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Genotypic differences observed among individuals in a population.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Any method used for determining the location of and relative distances between genes on a chromosome.
Highly repeated sequences, 6K-8K base pairs in length, which contain RNA polymerase II promoters. They also have an open reading frame that is related to the reverse transcriptase of retroviruses but they do not contain LTRs (long terminal repeats). Copies of the LINE 1 (L1) family form about 15% of the human genome. The jockey elements of Drosophila are LINEs.
Nucleotide sequences of a gene that are involved in the regulation of GENETIC TRANSCRIPTION.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.
Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).
The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
A discipline concerned with studying biological phenomena in terms of the chemical and physical interactions of molecules.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
DNA analogs containing neutral amide backbone linkages composed of aminoethyl glycine units instead of the usual phosphodiester linkage of deoxyribose groups. Peptide nucleic acids have high biological stability and higher affinity for complementary DNA or RNA sequences than analogous DNA oligomers.
A genus of shallow-cupped SPONGES with a broad base in the family Theonellidae. They are characterized by ectosomal spicules dominated by phyllotriaenes.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Peptides generated from AMYLOID BETA-PEPTIDES PRECURSOR. An amyloid fibrillar form of these peptides is the major component of amyloid plaques found in individuals with Alzheimer's disease and in aged individuals with trisomy 21 (DOWN SYNDROME). The peptide is found predominantly in the nervous system, but there have been reports of its presence in non-neural tissue.
A degenerative disease of the BRAIN characterized by the insidious onset of DEMENTIA. Impairment of MEMORY, judgment, attention span, and problem solving skills are followed by severe APRAXIAS and a global loss of cognitive abilities. The condition primarily occurs after age 60, and is marked pathologically by severe cortical atrophy and the triad of SENILE PLAQUES; NEUROFIBRILLARY TANGLES; and NEUROPIL THREADS. (From Adams et al., Principles of Neurology, 6th ed, pp1049-57)
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
... antisense elements (genetics) MeSH D27.720.470.530.600.150.200 - dna, antisense MeSH D27.720.470.530.600.150.200.640 - ... antisense MeSH D27.720.470.530.600.150.760.645 - oligoribonucleotides, antisense MeSH D27.720.470.530.600.223 - dna probes MeSH ... antisense MeSH D27.720.470.530.600.150.640.645 - oligoribonucleotides, antisense MeSH D27.720.470.530.600.150.760 - rna, ... oligodeoxyribonucleotides, antisense MeSH D27.720.470.530.600.150.640 - oligonucleotides, antisense MeSH D27.720.470.530. ...
Saberi F, Kamali M, Najafi A, Yazdanparast A, Moghaddam MM (2016-07-28). "Natural antisense RNAs as mRNA regulatory elements in ... focus on natural antisense transcripts". Trends in Genetics. 28 (8): 389-396. doi:10.1016/j.tig.2012.03.013. PMC 3768148. PMID ... Antisense RNA (asRNA), also referred to as antisense transcript, natural antisense transcript (NAT) or antisense ... Cis-natural antisense transcript Pelechano V, Steinmetz LM (December 2013). "Gene regulation by antisense transcription". ...
Genetics. 177 (3): 1621-37. doi:10.1534/genetics.106.061812. PMC 2147945. PMID 17720939. Carmi, Ilil; Kopczynski, Jennifer B.; ... These work in combination with Tsix, which is non-coding RNA that is an antisense which downregulates the effects of Xist on ... More recently, Meyer's lab has shown that proteins known as X-linked signal elements (XSEs) operate in concert with SDC-2 to ... Lee, Jeannie; Davidow, Lance S; Warshawsky, David (April 1999). "Tsix, a gene antisense to Xist at the X-inactivation centre". ...
Jurka J (December 2004). "Evolutionary impact of human Alu repetitive elements". Current Opinion in Genetics & Development. 14 ... The regions coding miRNA can be independent RNA-genes often being anti-sense to neighboring protein-coding genes, or can be ... Alu elements, short-interspersed nuclear element of about 300 nucleotides, are the most common SINE in humans, with >1,000,000 ... Short interspersed nuclear elements (SINEs) are non-autonomous, non-coding transposable elements (TEs) that are about 100 to ...
doi:10.1534/genetics.106.069484. PMC 1894591. PMID 17409075. Vanhée-Brossollet C, Vaquero C (1998). "Do natural antisense ... It is thought that in malignant cancer cells with activated transposable elements creates a large amount of transcriptional ... The NATsdb or Natural Antisense Transcript database is a rich tool for searching for antisense pairs from multiple organisms. ... Li YY, Qin L, Guo ZM, Liu L, Xu H, Hao P, Su J, Shi Y, He WZ, Li YX (2006). "In silico discovery of human natural antisense ...
Summerton J, Weller D (June 1997). "Morpholino antisense oligomers: design, preparation, and properties". Antisense & Nucleic ... Nasevicius A, Ekker SC (October 2000). "Effective targeted gene 'knockdown' in zebrafish". Nature Genetics. 26 (2): 216-20. doi ... "Correction of aberrant FGFR1 alternative RNA splicing through targeting of intronic regulatory elements". Human Molecular ... Penn AC, Balik A, Greger IH (January 2013). "Steric antisense inhibition of AMPA receptor Q/R editing reveals tight coupling to ...
... "elements" (enhancers and silencers) on the pre-mRNA transcript itself. These proteins and their respective binding elements ... Sazani P, Kang SH, Maier MA, Wei C, Dillman J, Summerton J, Manoharan M, Kole R (October 2001). "Nuclear antisense effects of ... Genetics. 7 (3): 211-21. doi:10.1038/nrg1807. PMID 16485020. S2CID 33672491. Clancy S (2008). "RNA Splicing: Introns, Exons and ... such as by bringing together splicing elements or by masking a sequence that would otherwise serve as a binding element for a ...
Lee JT, Davidow LS, Warshawsky D (April 1999). "Tsix, a gene antisense to Xist at the X-inactivation centre". Nature Genetics. ... "A proximal conserved repeat in the Xist gene is essential as a genomic element for X-inactivation in mouse". Development. 136 ( ... Graves JA (1996). "Mammals that break the rules: genetics of marsupials and monotremes". Annual Review of Genetics. 30: 233-60 ... Nature Reviews Genetics. (10) 155-159. Hoki Y, Kimura N, Kanbayashi M, Amakawa Y, Ohhata T, Sasaki H, Sado T (January 2009). " ...
... antisense elements (genetics) MeSH D13.444.600.150.200 - dna, antisense MeSH D13.444.600.150.200.640 - ... antisense MeSH D13.444.600.150.640.645 - oligoribonucleotides, antisense MeSH D13.444.600.150.760 - rna, antisense MeSH D13.444 ... MeSH D13.150.200.640 - oligodeoxyribonucleotides, antisense MeSH D13.150.480.640 - oligodeoxyribonucleotides, antisense MeSH ... antisense MeSH D13.444.735.150.319 - micrornas MeSH D13.444.735.150.640 - oligoribonucleotides, antisense MeSH D13.444.735.150. ...
Alu elements in humans and analogous B1 and B2 elements in mice have succeeded in becoming the most abundant mobile elements ... August 2004). "Antisense intronic non-coding RNA levels correlate to the degree of tumor differentiation in prostate cancer". ... Genetics. 17 (10): 601-614. doi:10.1038/nrg.2016.85. ISSN 1471-0064. PMID 27573374. Johnsson, Per; Lipovich, Leonard; Grandér, ... The abundance and distribution of Alu elements and similar repetitive elements throughout the mammalian genome may be partly ...
Rodriguez-Jato S, Nicholls RD, Driscoll DJ, Yang TP (2005). "Characterization of cis- and trans-acting elements in the ... Human Molecular Genetics. 5 (4): 517-24. doi:10.1093/hmg/5.4.517. PMC 6057871. PMID 8845846. Poukka H, Aarnisalo P, Santti H, ... "The IC-SNURF-SNRPN transcript serves as a host for multiple small nucleolar RNA species and as an antisense RNA for UBE3A". ... Human Molecular Genetics. 10 (23): 2687-700. doi:10.1093/hmg/10.23.2687. PMID 11726556. Runte M, Kroisel PM, Gillessen-Kaesbach ...
... "elements" (enhancers and silencers) on the pre-mRNA transcript itself. These proteins and their respective binding elements ... "Trends in Genetics. 30 (8): 340-347. doi:10.1016/j.tig.2014.05.005. PMC 4112133. PMID 24951248.. ... Splicing events can be experimentally altered[34][35] by binding steric-blocking antisense oligos such as Morpholinos or ... such as by bringing together splicing elements or by masking a sequence that would otherwise serve as a binding element for a ...
"PLOS Genetics: Search Results FANTOM3". journals.plos.org. de Hoon, Michiel; Hayashizaki, Yoshihide (April 2008). "Deep cap ... The non-coding RNA were identified as antisense RNA and long non-coding RNAs (lncRNA), poorly understood classes of regulatory ... Kazazian, H. H. (12 March 2004). "Mobile Elements: Drivers of Genome Evolution". Science. 303 (5664): 1626-1632. doi:10.1126/ ... A jointly released publication found that many of the transcripts in these forests show antisense transcription, and that most ...
Airn is the antisense gene of Ifg2r, so if Airn is being transcribed, the transcriptional machinery may cover a part of or the ... Trans acting elements, however, are gene products from one location that act on a different chromosome, either the other in a ... "Russel Silver Syndrome." Genetics Home Reference. US National Library of Medicine, Apr. 2008. Web. 09 May 2015. "Prader Willi ... Recent research has shown that a repeat element in the RNA of Xist causes PRC2 to bind to the RNA. Another part of the RNA ...
... are class I transposable elements in the DNA of some organisms and belong to the group of long interspersed nuclear elements ( ... The massive antisense expression of RT domain of ORF2 has been reported in primitive eukaryote Entamoeba histolytica which ... Ostertag EM, Kazazian HH (December 2001). "Biology of mammalian L1 retrotransposons". Annual Review of Genetics. 35 (1): 501-38 ... A typical L1 element is approximately 6,000 base pairs (bp) long and consists of two non-overlapping open reading frames (ORF) ...
Genetics. 166 (3): 1437-50. doi:10.1534/genetics.166.3.1437. PMC 1470764. PMID 15082561. Podlevsky JD, Bley CJ, Omana RV, Qi X ... This antisense-based process involves steps that first process the RNA so that it can base-pair with a region of its target ... Yu Q, Morrow CD (May 2001). "Identification of critical elements in the tRNA acceptor stem and T(Psi)C loop necessary for human ... 2015). "Small RNAs in bacteria and archaea: who they are, what they do, and how they do it". Advances in genetics (Vol. 90, pp ...
... including cancer genetics research, phylogenetic research, and human genetics. For example, using the 16S rRNA gene, which is ... The loci of Alu element insertion is selected, amplified and evaluated in terms of size of the fragment. The sex assay utilizes ... and two for the antisense strand. The primers bind adjacent to one another, forming a segment of double stranded DNA that once ... Nature Genetics. 6 (2): 130-5. doi:10.1038/ng0294-130. PMID 8162066. Rui, Lixin; Emre, N.C. Tolga; Kruhlak, Michael J.; Chung, ...
Antisense Antisense RNA Antisense strand Antisense therapy AP endonuclease AP site Apert syndrome Apoptosis Applied genetics ... site P element P1 P53 Palindrome Panmictic Paralogous genes Paramecin Parameters Parental Parental ditype Parental imprinting ... 2000), Chapter 1 (Genetics and the Organism): Introduction List of genetics research organizations List of geneticists & ... Genetic testing Genetic variance Genetic variation Geneticist Genetics Genetics and Archaeogenetics of South Asia Genetics ...
Genes ratA and txpA are found within a 48kb phage-like element called skin. This element interrupts a gene for the sigma factor ... Genetics. 172 (2): 1309-1323. doi:10.1534/genetics.105.042895. PMC 1456228. PMID 16299387. Retrieved 2010-08-13. Page for RNA ... Lee JM, Zhang S, Saha S, Santa Anna S, Jiang C, Perkins J (December 2001). "RNA expression analysis using an antisense Bacillus ... The toxin-antitoxin system contained within skin forces the inheritance of this element, which is acting as a selfish gene. The ...
"Chromosome 10". Genetics Home Reference. Retrieved 2017-05-06. "Chromosome 10". Human Genome Project Information Archive 1990- ... VIM antisense RNA 1 WASHC2C: WASH complex subunit 2C WBP1L: WW domain binding protein 1-like ZNF37A: zinc finger protein 37A ... non-SMC element 4 homolog A OTUD1: encoding protein OTU deubiquitinase 1 PAPSS2: encoding enzyme bifunctional 3'- ... Tom Strachan; Andrew Read (2 April 2010). Human Molecular Genetics. Garland Science. p. 45. ISBN 978-1-136-84407-2. Genome ...
This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding ... Tortora G, Clair T, Cho-Chung YS (Jan 1990). "An antisense oligodeoxynucleotide targeted against the type II beta regulatory ... Cytogenetics and Cell Genetics. 45 (3-4): 237-9. doi:10.1159/000132461. PMID 3691190. Budillon A, Cereseto A, Kondrashin A, ... Luo Z, Singh IS, Fujihira T, Erlichman J (Dec 1992). "Characterization of a minimal promoter element required for transcription ...
... (for HOX transcript antisense RNA) is a human gene located between HOXC11 and HOXC12 on chromosome 12. It is the first ... A subsequent study identified HOTAIR has 32 nucleotide long conserved noncoding element (CNE) that has a paralogous copy in ... Petherick A (August 2008). "Genetics: The production line". Nature. 454 (7208): 1042-5. doi:10.1038/4541042a. PMID 18756228. ... April 2020). "Ancestrally Duplicated Conserved Noncoding Element Suggests Dual Regulatory Roles of HOTAIR in cis and trans". ...
"A unified classification system for eukaryotic transposable elements". Nature Reviews. Genetics. 8 (12): 973-82. doi:10.1038/ ... Liang KH, Yeh CT (May 2013). "A gene expression restriction network mediated by sense and antisense Alu sequences located on ... SVA elements are present at lower levels than SINES and LINEs in humans. The starts of SVA and Alu elements are similar, ... SVA elements are the exception between the two as they share similarities with both LINEs and SINEs, containing Alu elements ...
Maeda T, Sepe P, Lahousse S, Tamaki S, Enjoji M, Wands JR, de la Monte SM (May 2003). "Antisense oligodeoxynucleotides directed ... August 2004). "Junctate is a key element in calcium entry induced by activation of InsP3 receptors and/or calcium store ... Molecular Genetics and Metabolism. 69 (3): 252-8. doi:10.1006/mgme.2000.2966. PMID 10767180. Dinchuk JE, Henderson NL, Burn TC ... August 2002). "Antisense oligonucleotides selectively regulate aspartyl beta-hydroxylase and its truncated protein isoform in ...
The MYCN gene has an antisense RNA, N-cym or MYCNOS, transcribed from the opposite strand which can be translated to form a ... March 2013). "The genetic landscape of high-risk neuroblastoma" (PDF). Nature Genetics. 45 (3): 279-84. doi:10.1038/ng.2529. ... "Differential effects of the widely expressed dMax splice variant of Max on E-box vs initiator element-mediated regulation by c- ... It has been shown that the antisense RNA encodes for a protein, named NCYM, that has originated de novo and is specific to ...
Just two P elements in the telomere are enough to suppress over 80 other copies of the P element in the genome. The cytoplasmic ... Most piRNAs are antisense to mRNAs transcribed from the silenced transposons, generally associating with Piwi and Aubergine ( ... PLoS Genetics. 3 (9): 1633-43. doi:10.1371/journal.pgen.0030158. PMC 1976332. PMID 17941712. Khurana, Jaspreet; Theurkauf, ... The P elements have an extremely high transposition rate and induce sterility and abnormal gonad development in D. melanogaster ...
Along with antisense TY1 transcripts, PHO84 antisense transcripts can serve a potential regulatory function in S. Cerevisiae. ... There has been an observed similarity between the suppression of the transposable element TY1 in yeast and small interfering ... Trends in Genetics. 21 (2): 93-102. doi:10.1016/j.tig.2004.12.009. PMID 15661355. Neil H; et al. (2009). "Widespread ... In XRN1 mutants, TY1 transcripts decrease in number and TY1 antisense transcripts increase. These antisense TY1 transcripts ...
Antisense therapy utilizes antisense oligonucleotides that bind to DUX4 messenger RNA, inducing its degradation and preventing ... The genetics of FSHD is unique and complex, centering around the DUX4 gene. Normally, DUX4 is expressed during embryogenesis ... Some transcripts that originate centromeric to the D4Z4 repeat array at the non-deleted element (NDE), termed D4Z4 regulatory ... Multiple RNA transcripts are produced from the D4Z4 repeat array, both sense and antisense. Some transcripts might be degraded ...
Alu repetitive element is member of Short Interspersed Elements (SINE) in mammalian genome. There are about 300 to 500 thousand ... There are three method are developed for this purpose: gene replacement, sense and anti-sense suppression, and insertional ... "Facilitating genome navigation: survey sequencing and dense radiation-hybrid gene mapping." Nature Reviews Genetics 6.8 (2005 ... However, using transposable element can bring more advantages. Transposable elements were first discovered by Barbara ...
... but could be a computational element in the cerebellum and the brain.[34] Indeed, an ouabain block of Na+. -K+. pumps in the ... using in particular anti-sense oligonucleotides.[44] ... Collaborative Study on the Genetics of Alcoholism. *College ... "The sodium-potassium pump is an information processing element in brain computation". Frontiers in Physiology. 5 (472): 472. ...
If one strand of the double-stranded DNA is considered the sense strand, then the other strand, considered the antisense strand ... of box ACA small nucleolar RNAs is defined by an evolutionarily conserved secondary structure and ubiquitous sequence elements ... "Genetics Home Reference. 16 March 2015.. *^ "Genetic Testing". nih.gov.. *^ "Definitions of Genetic Testing". Definitions of ... "antisense" sequence which is by itself nonfunctional, but can bind to the sense strand. ...
RNAi is now known as precise, efficient, stable and better than antisense therapy for gene suppression.[2] However, antisense ... "Nature Genetics. 36 (11): 1174-80. doi:10.1038/ng1452. PMID 15475954.. *^ Sugiyama T, Cam H, Verdel A, Moazed D, Grewal SI ( ... The 3'-UTR often contains microRNA response elements (MREs). MREs are sequences to which miRNAs bind. These are prevalent ... "Genetics and Molecular Biology. 34 (3): 363-70. doi:10.1590/S1415-47572011000300001. PMC 3168173. PMID 21931505.. ...
Lee JT, Davidow LS, Warshawsky D (April 1999). "Tsix, a gene antisense to Xist at the X-inactivation centre". Nature Genetics. ... "A proximal conserved repeat in the Xist gene is essential as a genomic element for X-inactivation in mouse". Development. 136 ( ... Graves JA (1996). "Mammals that break the rules: genetics of marsupials and monotremes". Annual Review of Genetics. 30: 233-60 ... Nature Reviews Genetics. (10) 155-159. *^ Hoki Y, Kimura N, Kanbayashi M, Amakawa Y, Ohhata T, Sasaki H, Sado T (January 2009 ...
Presence of repeated elements at its border and absence of promoter-associated sequences". Journal of Molecular Biology. 167 (1 ... Zhang MQ (May 1998). "Statistical features of human exons and their flanking regions". Human Molecular Genetics. 7 (5): 919-32 ... to pre-mRNA using Morpholino antisense oligos.[12] This has become a standard technique in developmental biology. Morpholino ...
He was a founding member of the Basic Sciences Division, and professor of genetics at UW. As described in an essay by Marc ... Weintraub, HM (1990). "Antisense RNA and DNA". Sci Am. 262: 40-46. doi:10.1038/scientificamerican0190-40. Lassar, AB; Paterson ... Weintraub, H; Hauschka, S; Tapscott, SJ (1991). "The MCK enhancer contains a p53 responsive element". Proc Natl Acad Sci USA. ... Another of his contributions was developing the technique of using antisense RNA to create specific mutant phenotypes in ...
"Genetics. 38 (6): 579-99. PMC 1209627. PMID 17247459.. *^ Des Jardins J (2010). The Madame Curie Complex: The Hidden History of ... Supposedly, the 5' LINE1 UTR that codes for the sense promoter for LINE1 transcription also encodes the antisense promoter for ... Activator element (Ac) is an example of an autonomous TE, and dissociation elements (Ds) is an example of a non-autonomous TE. ... Transposable elements represent one of several types of mobile genetic elements. TEs are assigned to one of two classes ...
doi:10.1534/genetics.113.152710. PMC 3730909. PMID 23709638.. *^ Bassett AR, Tibbit C, Ponting CP, Liu JL (July 2013). "Highly ... A transposon-like element called casposon encoding the Cas1-like integrase and potentially other components of the adaptation ... By designing an anti-virus CRISPR, they demonstrated that two orientations of the crRNA (sense/antisense) provided immunity, ... "Trends in Genetics. 30 (3): 111-8. doi:10.1016/j.tig.2014.01.003. PMC 3981743. PMID 24555991.. ...
Some promoters contain one or more upstream promoter element (UP element) subsites[5] (consensus sequence 5'-AAAAAARNR-3' when ... region of the anti-sense strand). Promoters can be about 100-1000 base pairs long.[1] ... and a B recognition element (BRE), which is recognized by the general transcription factor TFIIB.[4][7][8] The TATA element and ... Elements[edit]. Eukaryotic[edit]. *Core promoter - the minimal portion of the promoter required to properly initiate ...
... "antisense" sequence. Both sense and antisense sequences can exist on different parts of the same strand of DNA (i.e. both ... Schultz M, Cannon Z (2009). The Stuff of Life: A Graphic Guide to Genetics and DNA. Hill and Wang. ISBN 978-0-8090-8947-5. .. ... The atoms in the structure are colour-coded by element and the detailed structures of two base pairs are shown in the bottom ... "Trends in Genetics. 29 (2): 84-91. doi:10.1016/j.tig.2012.10.013. PMC 4378900. PMID 23158400.. ...
Each lighting element in the bioreactor is specially altered to emit specific wavelengths of light, as a full spectrum of light ... Antisense-expressing knockdown strains 1A6 and 1B1 contained 2.4- and 3.3-fold higher lipid content during exponential growth, ... VG Energy, a subsidiary of Viral Genetics Incorporated,[176] claims to have discovered a new method of increasing algal lipid ... Among all these elements being measured, phosphorus resulted in the most dramatic decrease, with a reduction of 84% over the ...
"Genetics. 188 (1): 11-20. doi:10.1534/genetics.111.128553. PMC 3120150. PMID 21546547. There is broad scientific consensus that ... Before the gene is inserted into the target organism it must be combined with other genetic elements. These include a promoter ... Kramer MG, Redenbaugh K (1 January 1994). "Commercialization of a tomato with an antisense polygalacturonase gene: The FLAVR ... For a non-technical introduction to the topic of genetics, see Introduction to genetics. For the song by Orchestral Manoeuvres ...
... viruses have been useful in the study of genetics and helped our understanding of the basic mechanisms of molecular genetics, ... V: (−)ssRNA viruses (− strand or antisense) RNA (e.g. Orthomyxoviruses, Rhabdoviruses). *VI: ssRNA-RT viruses (+ strand or ... transposons are examples of mobile genetic elements and could be the origin of some viruses. They were discovered in maize by ... or antisense (−). This classification places viruses into seven groups:. *I: dsDNA viruses (e.g. Adenoviruses, Herpesviruses, ...
Presence of repeated elements at its border and absence of promoter-associated sequences". Journal of Molecular Biology. 167 (1 ... Zhang MQ (May 1998). "Statistical features of human exons and their flanking regions". Human Molecular Genetics. 7 (5): 919-32 ... yang mengatur penjalinan ke pra-mRNA menggunakan oligomer antisense Morpholino.[12] Ini telah menjadi teknik standar dalam ...
Wagner E. G., Altuvia S., Romby P. (2002). "Antisense RNAs in bacteria and their genetic elements" (journal) (ingilis). 46 (Adv ... doi:10.1534/genetics.166.3.1437. PMID 15082561.. *↑ 1 2 Neveu M., Kim H. J., Benner S. A. (4 2013). "The "strong" RNA world ... Antisense RNT-lər bakteriyalarda geniş yayılmışdır, bir çoxları gen ifadəsini basdırır, lakin bəziləri ifadəni aktivləşdirir.[ ... Yu Q., Morrow C. D. (2001). "Identification of critical elements in the tRNA acceptor stem and TΨC loop necessary for human ...
"Genetics and Molecular Biology. 34 (3): 363-70. doi:10.1590/S1415-47572011000300001. PMC 3168173 . PMID 21931505.. ... The 3'-UTR often contains miRNA response elements (MREs). MREs are sequences to which miRNAs bind. These are prevalent motifs ... Recruitment of the small ribosomal subunit can indeed be modulated by mRNA secondary structure, antisense RNA binding, or ... or any other DNA-binding element to which a good antibody is available. ...
"Frontiers in Genetics. 7: 99. doi:10.3389/fgene.2016.00099. PMC 4893491. PMID 27375676.. ... Repression of transposable elements[edit]. DNA methylation is a powerful transcriptional repressor, at least in CpG dense ... These pitfalls have led to the development of antisense RNA therapies that target the DNMTs by degrading their mRNAs and ... "Forensic Science International: Genetics. 18: 21-32. doi:10.1016/j.fsigen.2014.11.015. ISSN 1872-4973.. ...
Wikimedia Commons has media related to Transcription (genetics).. Wikiversity has learning resources about Transcription ( ... The antisense strand of DNA is read by RNA polymerase from the 3' end to the 5' end during transcription (3' → 5'). The ... Key elements. *Ribosome. *Transfer RNA (tRNA). *Ribosome-nascent chain complex (RNC). *Post-translational modification ( ... Main article: Terminator (genetics). Bacteria use two different strategies for transcription termination - Rho-independent ...
"Genetics and Molecular Biology. 34 (3): 363-70. doi:10.1590/S1415-47572011000300001. PMC 3168173. PMID 21931505.. ... Mol JN, van der Krol AR (1991). Antisense nucleic acids and proteins: fundamentals and applications. M. Dekker. pp. 4, 136. ... Buchon N, Vaury C (February 2006). "RNAi: a defensive RNA-silencing against viruses and transposable elements". Heredity. 96 (2 ... "Human Molecular Genetics. 20 (R1): R21-7. doi:10.1093/hmg/ddr137. PMC 3095054. PMID 21459775.. ...
May 2008). "A universal classification of eukaryotic transposable elements implemented in Repbase". Nature Reviews Genetics. 9 ... Supposedly, the 5' LINE1 UTR that codes for the sense promoter for LINE1 transcription also encodes the antisense promoter for ... Activator element (Ac) is an example of an autonomous TE, and dissociation elements (Ds) is an example of a non-autonomous TE. ... Transposable elements represent one of several types of mobile genetic elements. TEs are assigned to one of two classes ...
"Genetics Home Reference. Retrieved 2017-05-06.. *. "Final genome 'chapter' published". BBC NEWS. 2006-05-18. Retrieved 2017-05- ... PACERR encoding protein PTGS2 antisense NFKB1 complex-mediated expression regulator RNA. *PBX1 (1q23) ... PGDB5: PiggyBac transposable element derived 5. *PIAS3 (1q21). *PI4KB: Phosphatidylinositol 4-kinase beta ... Tom Strachan; Andrew Read (2 April 2010). Human Molecular Genetics. Garland Science. p. 45. ISBN 978-1-136-84407-2. .. ...
Tijsterman M, Ketting RF, Plasterk RH (2002). "The genetics of RNA silencing". Annual Review of Genetics. 36: 489-519. doi: ... Lastly, the double stranded miRNAs/siRNAs separate into single strands; the antisense RNA strand of the two will combine with ... The 3'-UTR often contains microRNA response elements (MREs). MREs are sequences to which miRNAs bind. These are prevalent ... "Genetics and Molecular Biology. 34 (3): 363-70. doi:10.1590/S1415-47572011000300001. PMC 3168173. PMID 21931505.. ...
The Psi element is involved in viral genome packaging and recognized by gag and rev proteins. The SLIP element (TTTTTT) is ... Infection, Genetics and Evolution. 8 (3): 267-85. doi:10.1016/j.meegid.2008.01.002. PMID 18295550.. ... as well as DNA-dependent DNA polymerase activity that creates a sense DNA from the antisense cDNA.[68] Together, the cDNA and ... Tang J, Kaslow RA (2003). "The impact of host genetics on HIV infection and disease progression in the era of highly active ...
Cavaillé J, Nicoloso M, Bachellerie JP (1996). "Targeted ribose methylation of RNA in vivo directed by tailored antisense RNA ... Human Molecular Genetics 18 (12): 2140-2148. doi:10.1093/hmg/ddp137. ISSN 0964-6906. PMC 2685753. PMID 19304781. //www.ncbi.nlm ... of box ACA small nucleolar RNAs is defined by an evolutionarily conserved secondary structure and ubiquitous sequence elements ... "Sequence and structural elements of methylation guide snoRNAs essential for site-specific ribose methylation of pre-rRNA" ...
Wikimedia Commons has media related to Transcription (genetics).. *Interactive Java simulation of transcription initiation. ... The antisense strand of DNA is read by RNA polymerase from the 3' end to the 5' end during transcription (3' → 5'). The ... elements. *Mobilome *Horizontal gene transfer. *Genomic island. *Transposable element *Class I or retrotransposon ... Main article: Terminator (genetics). Bacteria use two different strategies for transcription termination - Rho-independent ...
For example, the nematode worm C. elegans, a commonly used model organism in genetics studies, has 29,647 [22] genes in its ... Jahn M, Rogers MJ, Söll D (July 1991). "Anticodon and acceptor stem nucleotides in tRNA(Gln) are major recognition elements for ... "Frontiers in Genetics. 5: 344. doi:10.3389/fgene.2014.00344. PMC 4189335. PMID 25339973.. ... "Frontiers in Genetics. 6: 271. doi:10.3389/fgene.2015.00271. PMC 4547532. PMID 26379699.. ...
... and NucleosidesAntisense Elements (Genetics)DNA, AntisenseOligodeoxyribonucleotides, AntisenseOligonucleotides, Antisense ... and NucleosidesNucleic AcidsNucleic Acid ProbesAntisense Elements (Genetics)DNA, AntisenseOligodeoxyribonucleotides, Antisense ... Genetics)DNA, AntisenseOligodeoxyribonucleotides, AntisenseOligonucleotides, AntisenseOligodeoxyribonucleotides, Antisense ... AntisenseOligoribonucleotides, AntisenseRNA, AntisenseOligoribonucleotides, Antisense. All MeSH CategoriesChemicals and Drugs ...
Antisense Elements (Genetics). Known as: Antisense Elements (Genetics) [Chemical/Ingredient], Anti-Sense Elements, Elements, ... Identification of antisense nucleic acid hybridization sites in mRNA molecules with self-quenching fluorescent reporter ... Nuclease resistance and RNA affinity are key criteria in the search for optimal antisense nucleic acid modifications, but the… ... HPV E6 antisense induces apoptosis in CaSki cells via suppression of E6 splicing ...
Amyloid beta-Peptides / genetics * Amyloid beta-Peptides / metabolism* * Antisense Elements (Genetics) * Binding Sites / ... Identification of amyloid-beta binding sites using an antisense peptide approach Neuroreport. 2001 Aug 8;12(11):2561-6. doi: ... Antisense peptide methodology has identified A beta recognition sequences and may also be applied to the identification of ... This study reports the use of antisense peptide methodology to identify specific A beta-binding domains. Synthetic peptides ...
... the extent to which they are involved in antisense regulation is unknown. Simultaneous expression of paired sense and antisense ... Is sense-antisense (SA) pairing of transcripts a common mode of gene regulation in the human genome? Although ,20% of human ... Antisense Elements (Genetics) * RNA, Antisense Grant support * CA 84405/CA/NCI NIH HHS/United States ... Simultaneous expression of paired sense and antisense genes is an essential step and an important indicator of antisense ...
Antigens, CD80 / genetics, immunology*. Antisense Elements (Genetics)*. Down-Regulation. Gene Transfer Techniques. Graft ... RESULTS: Antisense gene transfer resulted in markedly downregulation of B7.1 in the donor livers, attenuated acute immune ... CONCLUSIONS: Blocking expression of B7.1 in liver by antisense gene therapy may represent a potential strategy to attenuate ... METHODS: The liver grafts from 60 Dark Agouti rats were intraportally perfused with antisense B7.1 expression vector, before ...
Research Progress in Antisense Elements (Genetics). New York: Nova Biomedical Books, 2008, pp.87-123. ... Zinad HS, Natasya I, Werner A. Natural antisense transcripts at the interface between host genome and mobile genetic elements. ... Natural antisense transcripts (NATs). Large proportions of the human genome do not code for proteins and were long considered ... Translated anti-sense product of the Na/phosphate co- transporter (NaPi-II). Biochemical Journal 1998, 332, 483-489. ...
1999). "Cosuppression of I transposon activity in Drosophila by I-containing sense and antisense transgenes." Genetics 153(4): ... Another elements occurs to cause other types of Hybrid Dysgenesis. One of them is called P element, which are DNA transposons [ ... 1996). "Repression of hybrid dysgenesis in Drosophila melanogaster by heat-shock-inducible sense and antisense P-element ... High level of transposible element activity drives cell to death [85]. P element is under the control of RNA mediated mechanism ...
... active and acute lesions were studied for X2 MBP expression by in situ hybridization utilizing a synthetic oligonucleotide anti-sense ... active and acute lesions were studied for X2 MBP expression by in situ hybridization utilizing a synthetic oligonucleotide anti-sense ... active and acute lesions were studied for X2 MBP expression by in situ hybridization utilizing a synthetic oligonucleotide anti-sense ... active and acute lesions were studied for X2 MBP expression by in situ hybridization utilizing a synthetic oligonucleotide anti-sense ...
Repression of Hybrid Dysgenesis in Drosophila melanogaster by Heat-Shock-Inducible Sense and Antisense P-Element Constructs ... Submit an Article to GENETICS. GENETICS publishes high-quality scientific research that presents important findings in genetics ... P-Element-Induced Recombination in Drosophila melanogaster: Hybrid Element Insertion Yasmine H. M. Gray, Mark M. Tanaka, John A ... Excision Patterns of Activator (Ac) and Dissociation (Ds) Elements in Zea mays L.: Implications for the Regulation of ...
Both 35S-labeled RNA antisense and sense probes to amyloid β protein messenger RNA were used to ensure specific hybridization. ... Both 35S-labeled RNA antisense and sense probes to amyloid β protein messenger RNA were used to ensure specific hybridization. ... Both 35S-labeled RNA antisense and sense probes to amyloid β protein messenger RNA were used to ensure specific hybridization. ... Both 35S-labeled RNA antisense and sense probes to amyloid β protein messenger RNA were used to ensure specific hybridization. ...
1999b Cosuppression of I transposon activity in Drosophila by I-containing sense and antisense transgenes. Genetics 153: 1767- ... 1995 A role for the KP leucine zipper in regulating P element transposition in Drosophila melanogaster. Genetics 141: 587-594. ... 2000 P-element repression in Drosophila melanogaster by a naturally occurring defective telomeric P copy. Genetics 155: 1841- ... 1989 Modified P elements that mimic the P cytotype in Drosophila melanogaster. Genetics 123: 815-824. ...
Antisense Elements (Genetics)/genetics*. *Antisense Elements (Genetics)/pharmacology*. *Carcinoma/genetics. *Carcinoma/ ... The antisense construct for the 5-untranslated region with the most unique sequence for the L-plastin gene was more effective ... Suppression of prostate carcinoma cell invasion by expression of antisense L-plastin gene.. Zheng J1, Rudra-Ganguly N, Powell ... Cells infected with the 163-bp antisense virus, which were also tested in a nude mouse diaphragm invasion model, showed ...
Antisense Elements (Genetics) Medicine & Life Sciences * RNA Medicine & Life Sciences * Carbon Medicine & Life Sciences ... 2-O-alkyl oligoribonucleotides as antisense probes. A. M. Iribarren, B. S. Sproat, P. Neuner, I. Sulston, U. Ryder, A. I. ... 2-O-alkyl oligoribonucleotides as antisense probes. / Iribarren, A. M.; Sproat, B. S.; Neuner, P.; Sulston, I.; Ryder, U.; ... Iribarren, AM, Sproat, BS, Neuner, P, Sulston, I, Ryder, U & Lamond, AI 1990, 2-O-alkyl oligoribonucleotides as antisense ...
... antisense RNA explanation free. What is antisense RNA? Meaning of antisense RNA medical term. What does antisense RNA mean? ... Looking for online definition of antisense RNA in the Medical Dictionary? ... selection of target sites in RNA for antisense agents, and natural antisense RNA in mammals.. Antisense Elements (Genetics) ... antisense RNA. Also found in: Dictionary, Wikipedia. an·ti·sense RNA. the transcription product of the DNA antisense strand; it ...
Genetics. 1995;139:81-94. [PubMed]. 73. Petrov DA, Schutzman JL, Hartl DL, Lozovskaya ER. Diverse transposable elements are ... 1. Biemont C. A brief history of the status of transposable elements: from junk DNA to major players in evolution. Genetics. ... Positive and negative regulatory elements control expression of the yeast retrotransposon Ty3. Genetics. 1993;134:685-700. [ ... at the Ty1-DR3 element, in bas1Δ and bas1Δ tye7Δ cells. We previously showed that the 5′LTR of several endogenous Ty1 elements ...
Antisense Elements (Genetics). * Epigenesis, Genetic. * Natural Science Disciplines. * Phenomena and Processes. * Nucleic Acids ...
Antisense Elements (Genetics) - Preferred Concept UI. M0025053. Scope note. Nucleic acids which hybridize to complementary ... Anti Sense Elements. Anti Sense Probes. Anti-Sense Elements. Anti-Sense Probes. Antisense Probes. Elements, Anti-Sense. Probes ... Antisense Elements (Genetics) Entry term(s). Anti Sense Elements Anti-Sense Elements Elements, Anti-Sense ... GE genetics. HI history. IM immunology. IP isolation & purification. ME metabolism. PD pharmacology. PH physiology. PK ...
Comprehensive expressional analyses of antisense transcripts in colon cancer tissues using artificial antisense probes. Saito, ... Hirose, Y., Ikeda, K. T., Noro, E., Hiraoka, K., Tomita, M. & Kanai, A., 2015 7 14, : : BMC Genetics. 16, 1, 83.. 研究成果: Article ... Tamaki, S., Tomita, M., Suzuki, H. & Kanai, A., 2018 1 8, : : Frontiers in Genetics. 8, JAN, 227.. 研究成果: Article ... Comparative analysis of cis-encoded antisense RNAs in eukaryotes. Numata, K., Okada, Y., Saito, R., Kiyosawa, H., Kanai, A. & ...
Antisense Elements (Genetics) .. Anti-Sense Elements .. Anti-Sense Probes .. Anti Sense Elements .. Anti Sense Probes .. ... DNA, Antisense .. Anti-Sense DNA .. Anti Sense DNA .. DNA, Anti-Sense .. Antisense DNA .. DNA that is complementary to the ... RNA, Antisense .. Anti-Sense RNA .. Anti Sense RNA .. RNA, Anti-Sense .. Antisense RNA .. RNA molecules which hybridize to ... Elements, Anti-Sense .. Probes, Anti-Sense .. Probes, Antisense .. Antisense Probes .. Nucleic acids which hybridize to ...
Viral/genetics , Animals , Antibodies, Monoclonal , Antibodies, Viral , Antisense Elements (Genetics)/genetics , Chlorocebus ... Coronavirus/genetics , Spike Glycoprotein, Coronavirus/metabolism , Vero Cells , Viral Proteins/genetics , Viral Proteins/ ... Betacoronavirus/genetics , Betacoronavirus/isolation & purification , Clinical Laboratory Techniques/methods , Coronavirus ... Nucleocapsid Proteins/genetics , Nucleocapsid Proteins/metabolism , Pandemics , Phosphoproteins , Pneumonia, Viral/virology , ...
Adenoviridae , Genetics , Animals , Antisense Elements (Genetics) , Cell Line , Cell Proliferation , Genes, myc , Genetics , ... Adenoviridae , Genetics , Animals , Antisense Elements (Genetics) , Collagen Type I , DNA-Binding Proteins , Genetics , Liver ... Antisense Elements (Genetics) , Genetics , Bone Neoplasms , Genetics , Metabolism , Pathology , Cell Division , Humans , ... Animals , Antisense Elements (Genetics) , Genetics , Apoptosis , Cell Hypoxia , Cells, Cultured , Female , Male , Mitogen- ...
Antisense Elements (Genetics) Medicine & Life Sciences * human VEGFA protein Medicine & Life Sciences ... The antisense probe was designed to include a highly conversed region of the VEGF coding sequence and thus detect all known ... The antisense probe was designed to include a highly conversed region of the VEGF coding sequence and thus detect all known ... The antisense probe was designed to include a highly conversed region of the VEGF coding sequence and thus detect all known ...
Antisense Elements (Genetics) Medicine & Life Sciences * RNA Probes Medicine & Life Sciences * Digoxigenin Medicine & Life ... from the lumbar region of chick embryos were probed for the presence of trkB and p75 mRNA using digoxigenin-labeled anti-sense ... from the lumbar region of chick embryos were probed for the presence of trkB and p75 mRNA using digoxigenin-labeled anti-sense ... from the lumbar region of chick embryos were probed for the presence of trkB and p75 mRNA using digoxigenin-labeled anti-sense ...
Antisense Elements (Genetics) Medicine & Life Sciences * Oligonucleotide Probes Medicine & Life Sciences * Antisense ... Multiple fluorescein-labeled antisense oligonucleotide probes were designed to hybridize to the EBER1 transcript, which is ... Multiple fluorescein-labeled antisense oligonucleotide probes were designed to hybridize to the EBER1 transcript, which is ... Multiple fluorescein-labeled antisense oligonucleotide probes were designed to hybridize to the EBER1 transcript, which is ...
Antisense Elements (Genetics) Medicine & Life Sciences View full fingerprint Cite this. * APA ... The in situ RNA hybridization was carried out with digoxigenin-labeled sense and antisense RNA probes. Results. In all cases, ... The in situ RNA hybridization was carried out with digoxigenin-labeled sense and antisense RNA probes. Results. In all cases, ... The in situ RNA hybridization was carried out with digoxigenin-labeled sense and antisense RNA probes. Results. In all cases, ...
Antisense Elements (Genetics) Medicine & Life Sciences * Malignant Mixed Tumor Medicine & Life Sciences ... EBV transcripts were examined by in situ hybridization using digoxigenin-labeled oligonucleotide antisense probe for EBV- ... EBV transcripts were examined by in situ hybridization using digoxigenin-labeled oligonucleotide antisense probe for EBV- ... EBV transcripts were examined by in situ hybridization using digoxigenin-labeled oligonucleotide antisense probe for EBV- ...
Antisense Elements (Genetics) Medicine & Life Sciences * RNA Probes Medicine & Life Sciences * Sexual Development Medicine & ... It utilizes a 32P-labeled antisense RNA probe that is first hybridized in solution to tissue mRNA and then subjected to ... It utilizes a 32P-labeled antisense RNA probe that is first hybridized in solution to tissue mRNA and then subjected to ... It utilizes a 32P-labeled antisense RNA probe that is first hybridized in solution to tissue mRNA and then subjected to ...
Antisense Elements (Genetics) Medicine & Life Sciences View full fingerprint Cite this. * APA ... This represented approximately 12.5% of the microarray elements. In contrast, pertussis toxin altered only 103 (0.9%) elements ... This represented approximately 12.5% of the microarray elements. In contrast, pertussis toxin altered only 103 (0.9%) elements ... This represented approximately 12.5% of the microarray elements. In contrast, pertussis toxin altered only 103 (0.9%) elements ...
In contrast, nuclear retention is promoted by specialized cis-elements found in certain RNAs. This new understanding of the ... In contrast, nuclear retention is promoted by specialized cis-elements found in certain RNAs. This new understanding of the ... As described above, if a pair of transposable elements are found in the sense and anti-sense orientation in a single transcript ... Other cis-Elements That Promote Nuclear Retention. A few other cis-elements that promote nuclear retention have been ...
  • Is sense-antisense (SA) pairing of transcripts a common mode of gene regulation in the human genome? (nih.gov)
  • Our research mainly focuses on a particular family of noncoding RNAs, so-called natural antisense transcripts (NATs). (ncl.ac.uk)
  • Wight M, Werner A. The functions of natural antisense transcripts. (ncl.ac.uk)
  • Piatek MJ, Henderson V, Fearn A, Chaudhry B, Werner A. Ectopically expressed Slc34a2a sense-antisense transcripts cause a cerebellar phenotype in zebrafish embryos depending on RNA complementarity and Dicer . (ncl.ac.uk)
  • EBV transcripts were examined by in situ hybridization using digoxigenin-labeled oligonucleotide antisense probe for EBV-encoded RNA 1 (EBER1) on formalin-fixed paraffin- embedded tissue sections. (elsevier.com)
  • Nekrutenko & Li, 2001 ), and in supplying transcriptional start sites for antisense transcripts used in gene regulation ( Conley, Miller & Jordan, 2008 ). (peerj.com)
  • Natural antisense transcripts (NATs) are transcribed from the opposite strand to a protein coding or sense strand in the chromatin. (frontiersin.org)
  • Human cis natural antisense transcripts initiated by transposable elements. (semanticscholar.org)
  • 2011). The PAIR elements, which bind Pax5, E2A, CTCF, and cohesin, give rise to long noncoding antisense transcripts just in pro-B cells, recommending that they regulate distal VH-DJH recombination perhaps by managing locus compression (Ebert et al. (capecodmushroom.org)
  • LTRs can act as promoters, as sources of regulatory sequences, or initiate antisense transcripts regulating gene expression. (springer.com)
  • While long ncRNAs are located and transcribed within the intergenic stretches, the majority are transcribed as complex, interlaced networks of overlapping sense and antisense transcripts that often includes protein-coding genes. (wikipedia.org)
  • Genomic sequences within these transcriptional foci are often shared within a number of different coding and non-coding transcripts in the sense and antisense directions giving rise to a complex hierarchy of overlapping isoforms. (wikipedia.org)
  • Genomic regulatory elements also give rise to ncRNAs: transcription start site-associated short RNAs (TSSa-RNAs) or promoter upstream transcripts (PROMPTs) are produced from promoter regions, and eRNAs from enhancer regions. (prolekare.cz)
  • Endogenous ncRNA molecules, at least partially complementary to known protein-coding transcripts, were also discovered and named natural antisense transcripts (NATs). (prolekare.cz)
  • In particular, the important role played by natural antisense transcripts (NATs) in regulating the expression of genes is receiving increasing attention. (usda.gov)
  • Some specific topics examined include clinical application of RNAs for cancer therapy, selection of target sites in RNA for antisense agents, and natural antisense RNA in mammals. (thefreedictionary.com)
  • We also provide the first evidence that Ty1 antisense transcription can be regulated by environmental stress conditions, pointing to a new level of control of Ty1 activity by stress, as Ty1 antisense RNAs play an important role in regulating Ty1 mobility at both the transcriptional and post-transcriptional stages. (pubmedcentralcanada.ca)
  • Endogenous antisense RNAs function as regulators of gene expression by a variety of mechanisms. (bireme.br)
  • Synthetic antisense RNAs are used to effect the functioning of specific genes for investigative or therapeutic purposes. (bireme.br)
  • In contrast, nuclear retention is promoted by specialized cis -elements found in certain RNAs. (frontiersin.org)
  • These studies reveal novel insights into physiological regulation of natural antisense RNAs. (frontiersin.org)
  • This paper connects Alu repeats, the most abundant repetitive elements in the human genome and microRNAs, small RNAs that alter gene expression at the post-transcriptional level. (nih.gov)
  • For the P strain, the P-M phenotypes are associated with the ability to express a class of small RNAs, called piwi-interacting small RNAs (piRNAs), that suppress the P elements in female gonads. (biomedcentral.com)
  • Replication control can be either mediated by iterons or by antisense RNAs. (asmscience.org)
  • Antisense RNAs work through a negative control circuit. (asmscience.org)
  • In other cases, antisense RNAs inhibit the formation of an activator pseudoknot that is required for efficient Rep translation. (asmscience.org)
  • Acting antisense-plasmid- and chromosome-encoded small regulatory RNAs (sRNAs) from Gram-positive bacteria. (asmscience.org)
  • Antisense RNAs in bacteria and their genetic elements. (asmscience.org)
  • Moreover, piwi-interacting RNAs (piRNAs), in Drosophila as in mammals, are derived from heterochromatin domains containing mostly transposable elements and degenerate transposons, and are involved in transposon silencing. (prolekare.cz)
  • Both 35S-labeled RNA antisense and sense probes to amyloid β protein messenger RNA were used to ensure specific hybridization. (elsevier.com)
  • 2'-O-Methyl oligoribonucleotides have recently been introduced as antisense probes for studying RNA processing and for affinity purification of RNA-protein complexes. (dundee.ac.uk)
  • These properties indicate that the 2'-O-allyl oligoribonucleotides are particularly well suited for use as antisense probes. (dundee.ac.uk)
  • The corresponding digoxigenin-labeled antisense RNA probes were prepared from pGEM-1 containing a BamH1-EcoR1 956-bp insert consisting of exon 3 and flanking intron sequences from the rat IGF-1 gene, and the pTRI-IGFR-human transcription template containing a 236-bp cDNA fragment of the human IGF-1R gene spanning exons 8-7 (Ambion, Austin, TX, USA). (thefreedictionary.com)
  • Antisense and corresponding sense (control) RNA probes were transcribed from the pCR(TM)II construct (Invitrogen, San Diego, CA), which contained human VEGF cDNA. (elsevier.com)
  • Multiple fluorescein-labeled antisense oligonucleotide probes were designed to hybridize to the EBER1 transcript, which is highly expressed in latently infected cells. (utmb.edu)
  • The in situ RNA hybridization was carried out with digoxigenin-labeled sense and antisense RNA probes. (elsevier.com)
  • Several antisense DNA probes and an siRNA for latrophilin-2 produced a loss of EMT in collagen gel cultures. (elsevier.com)
  • Preliminary in situ hybridization studies in E12.5-13 mouse embryos with antisense probes from the 5' region of D7 demonstrate a ubiquitous expression pattern while antisense probe from the 3' region of the D7 gene demonstrate a more restricted expression pattern within the developing heart endothelium, the limb buds and the somite regions. (elsevier.com)
  • Probes for the flanking regions of the T-DNA and transposed Ds HPT elements were obtained with the inverse polymerase chain reaction (IPCR) technique and used in RFLP linkage analyses. (springer.com)
  • Piatek MJ, Henderson V, Zynad HS, Werner A. Natural antisense transcription from a comparative perspective . (ncl.ac.uk)
  • HOTAIR (HOX transcript antisense RNA ), which is 2.2 kb in length and is located on 12q13.13, is a long noncoding RNA formed by HOXC gene transcription. (thefreedictionary.com)
  • In one representation, the RNA synthesis from a DNA strand may hit the transcription of the other strand, suppressing the transcription of antisense RNA sense. (thefreedictionary.com)
  • We identify three Tye7 potential binding sites in the region of Ty1 DNA sequence where antisense transcription starts. (pubmedcentralcanada.ca)
  • We show that Tye7 binds to Ty1 DNA and regulates Ty1 antisense transcription. (pubmedcentralcanada.ca)
  • Altogether, our data suggest that, in response to adenylic nucleotide reduction, TYE7 is induced and activates Ty1 mRNA transcription, possibly by controlling Ty1 antisense transcription. (pubmedcentralcanada.ca)
  • Stress conditions generally stimulate transcription of the element, which is the first step of the transposition cycle, as shown with Tnt1A and TLC-1 in Solanaceae ( 3 , 7 ), Mutator in Maize ( 8 , 9 ), Tf2 in Schizosaccharomyces Pombe ( 10 ) and Ty1 in S. cerevisiae ( 11-18 ). (pubmedcentralcanada.ca)
  • Recent studies have provided ample evidence that more than 70% of the mammalian genome have antisense transcription potential. (frontiersin.org)
  • Despite efforts to unravel the specific role(s) of wide spread antisense transcription in recent years, the functional significance of NATs and their physiological regulation remains poorly understood. (frontiersin.org)
  • 2002) and antisense transcription in the VHJ558 gene region (Bolland et al. (capecodmushroom.org)
  • Since there were not enough students, the sense strand moved away, but the students were told that the opening of the helix and transcription actually occur simultaneously The sense-strand students exchanged their DNA codes for mRNA codons and moved back in line, one by one, to face the antisense strand , demonstrating that the antisense strand was the template for transcription. (thefreedictionary.com)
  • the presence of the -35 element appears to be unimportant for transcription from the UP element-containing promoters. (wikidoc.org)
  • If the inserted DNA includes a strong promoter, dominant mutants can be obtained by driving transcription into an adjacent gene, leading to the over-expression of an mRNA encoding a full-length or truncated protein, or an antisense RNA, depending the position and orientation of the inserted promoter. (pnas.org)
  • Antisense transcription in the mammalian transcriptome. (prolekarniky.cz)
  • 20% of human genes might form SA pairs, the extent to which they are involved in antisense regulation is unknown. (nih.gov)
  • Simultaneous expression of paired sense and antisense genes is an essential step and an important indicator of antisense regulation. (nih.gov)
  • It has also been exploited in GENETIC ENGINEERING , to produce, for example, TRANSGENIC tomatoes, in which the antisense RNA inactivates expression of genes coding for ENZYMES that are involved in the softening and rotting of the fruit. (thefreedictionary.com)
  • The work that won Mello, 45, and Fire, 47, the coveted prize began in the mid-1990s when they and others were experimenting with " antisense RNA ," a then-promising method for modulating the activity of genes inside cells. (thefreedictionary.com)
  • Generally, this process involves regulatory sequences located in the promoter region of transposable elements, which are similar to the well-characterized motifs required for the induction of stress-responsive genes ( 3 , 4 ). (pubmedcentralcanada.ca)
  • After treatment with antisense DNA against latrophilin-2, expression of 1,385 genes and ESTs was altered. (elsevier.com)
  • The study of the genetics of enterococci has focused heavily on mobile genetic elements present in these organisms, the complex regulatory circuits used to control their mobility, and the antibiotic resistance genes they frequently carry. (asmscience.org)
  • Nearly all eukaryotic genomes contain a substantial fraction of middle repetitive, transposable element (TE) sequences interspersed with the unique sequences encoding genes and cis -regulatory elements. (biomedcentral.com)
  • We have established a genetic strategy that uses parasexual genetics to allow essential Dictyostelium discoideum genes to be manipulated and replaced with modified or tagged homologues. (biomedcentral.com)
  • The large VH gene cluster contains 14 Pax5-activated intergenic repeat (PAIR) elements, which are interspersed together with the VH3609 genes in the distal VHJ558 gene region (Ebert et al. (capecodmushroom.org)
  • This is particularly true for LTR retrotransposons, whose Long Terminal Repeats (LTRs) promoter/regulatory capsules are present at both ends of the element and make them particularly prone to influencing adjacent genes. (springer.com)
  • When you consider all the problems we credit to things like "bad genes", you can start to understand why being able to address them through something like antisense oligonucleotides is so exciting. (discoverhealthnow.com)
  • The siRNA can form a molecular complex with proteins that first strip away the sense strand of RNA, making the antisense inhibitory RNA (iRNA) available for base pairing with messenger RNA (mRNA). (wikiversity.org)
  • Where the target is the RNA transcript of a gene (i.e. mRNA), copied from the SENSE STRAND of DNA , the antisense RNA is a counter-transcript, copied from the antisense strand (see ANTICODING STRAND of the DNA , and complementary to the mRNA. (thefreedictionary.com)
  • The antisense strand is the template for mRNA synthesis. (bireme.br)
  • It utilizes a 32 P-labeled antisense RNA probe that is first hybridized in solution to tissue mRNA and then subjected to digestion with ribonucleases. (elsevier.com)
  • Antisense RNA (asRNA), also referred to as antisense transcript, natural antisense transcript (NAT) or antisense oligonucleotide, is a single stranded RNA that is complementary to a protein coding messenger RNA (mRNA) with which it hybridizes, and thereby blocks its translation into protein. (wikipedia.org)
  • In other cases, the bound antisense strands catalyze specific chemical reactions that actively degrade the targeted mRNA. (thefreedictionary.com)
  • Interestingly, the zygotic P -element piRNAs, but not the KP element mRNA, contributed to the variations in P transposition immunity in the granddaughters. (biomedcentral.com)
  • Expression levels of the P -element piRNA and KP -element mRNA vary among F1 progeny due to the constitution of the paternal genome, and are involved in phenotypic variation in the subsequent generation. (biomedcentral.com)
  • To elucidate the molecular basis of the suppression of paternally inherited P elements, we analyzed the mRNA and piRNA levels of P elements in the F1 progeny between males of a P strain and nine-line females of M′ or Q strains (M′ or Q progenies). (biomedcentral.com)
  • Consistently, the levels of P -element mRNA in both the ovaries and F1 embryos were higher in M′ progenies than in Q progenies, indicating that the M′ progenies have a weaker ability to suppress P -element expression. (biomedcentral.com)
  • The level of P -element mRNA was inversely correlated to the level of piRNAs in F1 embryos. (biomedcentral.com)
  • If a primary piRNA has a sequence antisense to a TE, it can guide the cleavage of the mRNA of the TE. (biomedcentral.com)
  • Alternatively, ribosome binding to a leader peptide mRNA whose translation is required for efficient Rep translation can be prevented by antisense-RNA binding. (asmscience.org)
  • Geneticists weren't just happy learning this information, so they began the process of leveraging it to make antisense oligonucleotides. (discoverhealthnow.com)
  • Transposable elements constitute a large fraction of eukaryotic genomes (nearly half of the human genome, up to 85% of plant genomes and 3% of the compact genome of the yeast Saccharomyces cerevisiae as examples). (pubmedcentralcanada.ca)
  • The recent availability of genome sequences has provided unparalleled insights into the broad-scale patterns of transposable element (TE) sequences in eukaryotic genomes. (biomedcentral.com)
  • The notice of allowance issued by the Canadian Intellectual Property Office is for a patent that covers basic use of antisense RNA in all cells and cellular material (eukaryotic and prokaryotic alike), and broadly covers methods for regulating gene function in all cells using antisense RNA. (freethesaurus.com)
  • Transposable elements (TEs) are major structural constituents of eukaryotic genomes. (biomedcentral.com)
  • Transposable elements (TEs) occupy a substantial fraction of eukaryotic genomes, and their mobilization causes insertional mutations. (biomedcentral.com)
  • Implications for models of transposable element silencing are discussed. (genetics.org)
  • THE P -transposable element is a recent invader of natural populations of Drosophila melanogaster ( K idwell 1983 ). (genetics.org)
  • In this study, we showed that a previously identified putatively adaptive mutation, the insertion of the transposable element FBti0019627, mediates resistance to both natural and synthetic xenobiotics in Drosophila melanogaster. (prolekare.cz)
  • While SNPs certainly contribute to ecologically relevant phenotypes, these efforts ignore other types of mutations, such as those caused by transposable element (TE) insertions. (prolekare.cz)
  • We have introduced a genetically marked Dissociation transposable element ( Ds HPT ) into tomato ( Lycopersicon esculentum ) by Agrobacterium tumefaciens -mediated transformation. (springer.com)
  • Chuck G, Robbins T, Nijjar C, Ralston E, Courtney-Gutterson N, Dooner HK (1993) Tagging and cloning of a petunia flower color gene with the maize transposable element Activator . (springer.com)
  • Greenblatt IM (1984) A chromosomal replication pattern deduced from pericarp phenotypes resulting from movements of the transposable element Modulator in maize. (springer.com)
  • The newly allowed claims broadly cover, for example, double-stranded, RNAi-mediating molecules of between 19 and 52 nucleotides in length with a 3' overhang on the antisense strand , and thus include siRNA trigger designs such as 'dicer substrates. (thefreedictionary.com)
  • [7] However, it is noted that the specific sequence of intronic splicing elements and the number of nucleotides between the branchpoint and the nearest 3' acceptor site affect splice site selection. (wikipedia.org)
  • The sequence at -10 is called the [[Pribnow box]], or the -10 element, and usually consists of the six nucleotides TATAAT. (wikidoc.org)
  • The other sequence at -35 (the -35 element) usually consists of the six nucleotides TTGACA. (wikidoc.org)
  • These findings suggest that antisense regulation is a common and important mechanism of gene regulation in the human genome. (nih.gov)
  • Transposable elements play a fundamental role in genome evolution. (pubmedcentralcanada.ca)
  • Transposable elements are found abundantly in non-coding DNA and the Alu family of SINE transposons accounts for approximately 10% of the total DNA in the human genome ( Cordaux & Batzer, 2009 ). (peerj.com)
  • It also occurs when certain types of transposable genetic elements ( transposons ) copy themselves in preparation for reinserting elsewhere in the cell's genome. (encyclopedia.com)
  • In the course of our studies, we have discovered a novel promoter/enhancer element in the anti-sense genome of the virus, which is common to 10/11 AAV genome fragments implicated in the HCC study. (cam.ac.uk)
  • The article then focuses on conjugative transposons, now referred to as integrated, conjugative elements, or ICEs, and concludes with several smaller sections covering emerging areas of interest concerning the enterococcal mobilome, including nonpheromone plasmids of particular interest, toxin-antitoxin systems, pathogenicity islands, bacteriophages, and genome defense. (asmscience.org)
  • LTR Retrotransposons as Controlling Elements of Genome Response to Stress? (springer.com)
  • P -element transposition in the genome causes P-M hybrid dysgenesis in Drosophila melanogaster . (biomedcentral.com)
  • The piRNA-cluster-embedded P elements and the transcriptionally active KP elements from the paternal genome are both important suppressors of P element activities that are co-inherited by the progeny. (biomedcentral.com)
  • P elements are DNA transposons that propagate in the D. melanogaster genome and include both structurally complete and incomplete variants. (biomedcentral.com)
  • Transposition of P elements in the genome causes P-M hybrid dysgenesis in Drosophila melanogaster . (biomedcentral.com)
  • The P element is a DNA transposon, and their copies in the Drosophila melanogaster genome include structurally complete and incomplete variants. (biomedcentral.com)
  • Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project. (prolekarniky.cz)
  • Activation of transposable elements in response to stress conditions has been reported in a wide range of organisms ( 3-5 ) and has been proposed to promote genetic variability that could help the cell to adapt to environmental changes ( 6 ). (pubmedcentralcanada.ca)
  • Polymorphisms for Alu insertions have been much studied as a tool in human population genetic inference, particularly because the absence of the element can always be identified as the ancestral state ( Batzer & Deininger, 2002 ). (peerj.com)
  • Plasmids are selfish genetic elements that normally constitute a burden for the bacterial host cell. (asmscience.org)
  • The molecular cause of most epimutations is unknown but it is hypothesised that they could be driven by hitherto unidentified genetic alterations in long‐range cis ‐regulatory elements that manifest as DNA methylation or repressive histone modifications at a gene promoter. (els.net)
  • In cis DNA elements such as enhancers and insulators regulate gene expression and are potential sites of genetic alterations that give rise to epimutations. (els.net)
  • Genetic approaches to investigate signaling mechanisms fall into two broad categories: In forward genetics one creates random mutations in a population of cells, whereas in reverse genetics one manipulates a known gene. (pnas.org)
  • In forward genetics, mutations can be induced by using chemical mutagens, insertional mutagens or through the delivery of diverse libraries containing, for example, cDNAs, shRNAs, or genetic suppressor elements, with each approach creating distinct genetic changes ( 1 - 4 ). (pnas.org)
  • This genetic information is useful in scientific research, genealogy and simply a comforting element for survivors. (brighthub.com)
  • Antisense technology is still in its infancy, but may prove to be one of the most important breakthroughs in the battle with genetic disorders and diseases. (brighthub.com)
  • In other cases, the protein-coding RNA sense strand might be produced by a virus and the antisense RNA strand produced by the host cell. (wikiversity.org)
  • The sense and antisense RNA strands form double strand RNA (Figure 2, top) that is processed to small (about 20 base pairs long) inhibitory RNA (siRNA). (wikiversity.org)
  • See also antisense strand . (thefreedictionary.com)
  • The filter for the number of A/U in the 5'-end pentamer of the antisense strand (f-AU). (thefreedictionary.com)
  • Two points are gained if the 5'-end antisense strand of a siRNA sequence starts with U. (thefreedictionary.com)
  • This differential stability ensures that the antisense strand is incorporated into the RISC complex, reducing the unwanted off-target effect caused by the sense-strand [10,17,19,21,24,27,28,29]. (thefreedictionary.com)
  • To trick a gene into making antisense RNA that complements the RNA it produces normally, biologists take advantage of the natural antisense strand that forms the quiet half of the DNA double helix. (thefreedictionary.com)
  • To switch the roles of the two helix partners, researchers splice a promoter into the beginning of the sequence, then flop that piece of DNA so that the sense strand extends backward and the antisense strand extends forward. (thefreedictionary.com)
  • The filter for the [DELTA]G (free energy) at the 5'-end of the antisense strand (f-dga). (thefreedictionary.com)
  • Asymmetric amplification of the antisense strand occurred in 10 [micro]L of 50 mmol/L Tris, pH 8. (thefreedictionary.com)
  • The resulting primary RNA strand is complementary to the DNA antisense strand and is identical to the nucleotide sequence expressed by the sense DNA strand, except that the nucleotide uridine (U) replaces T during complementary base pairing (A-U in RNA, A-T in DNA) [ILLUSTRATION FOR FIGURE 2 OMITTED]. (thefreedictionary.com)
  • Chimeric RNA-DNA duplexes (Patent Pending): These novel RNAi-like compounds, comprised of a RNA antisense strand and a modified DNA sense strand, were designed to provide potential specificity advantages compared with first generation siRNA. (thefreedictionary.com)
  • The first product, now on the market, amplifies the antisense strand of RNA. (thefreedictionary.com)
  • Exonization of Alu elements creates primate-specific genomic diversity. (psu.edu)
  • To elucidate the molecular basis of P -element suppression by zygotic factors, we investigated the genomic constitution and P -element piRNA production derived from fathers. (biomedcentral.com)
  • The study identified genomic elements that were present in COL but absent from the strains of interest. (asm.org)
  • Topics covered range from rearing embryos to the care of adult animals, while also presenting the basic experimental methods including light and electron microscopy, used to study gene expression, transgenics, reverse genetics, and genomic approaches. (elsevier.com)
  • 2009). Expression of the Plasmodium berghei actin II gene is controlled by elements in a long genomic region. (communitycinema.org)
  • Increased plasmid copy numbers lead to increasing antisense-RNA concentrations, which, in turn, result in the inhibition of a function essential for replication. (asmscience.org)
  • Conversely, in vivo inhibition of CYP46A1 in the mice, using antisense RNA molecules delivered by an AAV vector administered to the hippocampus, induces an increase in the production of Aß peptides, abnormal tau protein, neuronal death and hippocampal atrophy, leading to memory problems. (medicalnewstoday.com)
  • The idea of asRNAs as drug targets started in 1978 when Zamecnik and Stephenson found an antisense oligonucleotide to the viral RNA of Rous scarcoma virus that was capable of inhibiting viral replication and protein synthesis. (wikipedia.org)
  • Here we searched for natural antisense transcript for sensorin (NAT-SRN), a neuropeptide expressed in the presynaptic sensory neurons of gill-withdrawal reflex of the marine snail Aplysia californica . (frontiersin.org)
  • This overlapping gene organization, which produces sense-antisense transcript pairs, is capable of affecting regulatory cascades through established mechanisms. (frontiersin.org)
  • This is of great interest as Alu is a retro-element that can transpose in a cycle containing a free Alu transcript, which is always in sense orientation. (nih.gov)
  • To identify RNA analogues with improved properties for antisense analysis, 2'-O-alkyl oligoribonucleotides were synthesized in which the alkyl moiety was either the three-carbon linear allyl group or the five-carbon branched 3,3-dimethylallyl group. (dundee.ac.uk)
  • The antisense construct for the 5'-untranslated region with the most unique sequence for the L-plastin gene was more effective in down-regulation efficiency compared with the larger antisense construct in the coding region, which maintains homology to other members of the plastin gene family. (nih.gov)
  • The antisense probe was designed to include a highly conversed region of the VEGF coding sequence and thus detect all known variants. (elsevier.com)
  • This yielded all Alu elements without and with an overlap of at least one expressed sequence tag (EST), in the case of the intronic and exonic data sets, respectively. (psu.edu)
  • An analysis of C. elegans transposable elements =-=[25,26]-=- revealed that a 205-bp ITR sequence within Turmoil-1 is highly similar to a region of two exons separated by an intron of the rsp-2 gene (see pairwise alignment using bl2seq [27] Figure 1B). (psu.edu)
  • Initial studies into lncRNA conservation noted that as a class, they were enriched for conserved sequence elements, depleted in substitution and insertion/deletion rates and depleted in rare frequency variants, indicative of purifying selection maintaining lncRNA function. (wikipedia.org)
  • To determine the optimal concentration of c-erbB2 antisense probe labeled with superparamagnetic iron oxide (SPIO) nanoparticles for in vivo tumor imaging in mice using magnetic resonance imaging (MRI). (bvsalud.org)
  • Thirty BALB/c mice bearing SK-Br-3 tumor were randomized into 5 groups to receive injections of different concentrations of SPIO-labeled c-erbB2 antisense probe (containing 6.0, 9.0, 12.0, 15.0, or 18.0 mg Fe/kg). (bvsalud.org)
  • Nature Genetics 2015). (cam.ac.uk)
  • The world's first wiki where authorship really matters (Nature Genetics, 2008). (wikigenes.org)
  • The ability of the P -element family to repress its own activity was first interpreted as resulting from the synthesis of polypeptides encoded by various P elements. (genetics.org)
  • Another antisense molecule, this one directed against the RNA involved in the synthesis of a growth-regulating hormone, may prove useful against chronic myelogenous leukemia, says Alan M. (freethesaurus.com)
  • From linear expression elements (lee\'s) to gene synthesis, things will never be the same thanks to the scientific minds that are hard at work behind the scenes. (discoverhealthnow.com)
  • In contrast, when P -strain females are mated with P-strain males, P- element mobilization is prevented by maternally deposited piRNAs in the germline cells and early embryos, which are laid by P-strain mothers but not P-progeny mothers (referred to as F1 embryos of P progenies) [ 21 ]. (biomedcentral.com)
  • Researchers can order primers to investigate the SNP of interest on both the sense and antisense strands as well as explore whether the template DNA is heterozygous or homozygous for a given allele. (thefreedictionary.com)
  • in Woodlands, Texas, have designed antisense strands of DNA that migrate directly into the cell's nucleus. (thefreedictionary.com)
  • Researchers say they have just begun to assess the behavior and safety of so-called second-and third-generation oligos -- antisense strands chemically modified for enhanced stability or therapeutic potential in the body. (thefreedictionary.com)
  • Scientists have long since known that DNA contains two separate strands, one known as the sense and the other known as the antisense. (discoverhealthnow.com)
  • A variety of transcriptional and posttranscriptional mechanisms of regulation of gene expression are then discussed, including a section on the genetics and regulation of vancomycin resistance in enterococci. (asmscience.org)
  • Transposable elements can impact gene expression and regulatory patterns. (springer.com)
  • They typically lie upstream of the gene and can have regulatory elements several kilobases away from the transcriptional start site. (wikidoc.org)
  • Altered expression of sRNA and their gene targets, in response to abiotic and biotic stress have firmly established the importance of these regulatory elements. (frontiersin.org)
  • In vitro invasion and motility of PC-3 and PC-3M cells were drastically suppressed (approximately 10-fold) by the expression of the antisense constructs. (nih.gov)
  • The technique is certainly structured on a combinatorial strategy of reprogramming elements mixed with a blend of episomal vectors that make IPSC without the want for and and and Myc/Lin28-free of charge constructs in the existence and lack of reprogramming elements. (cenicriviroc.info)
  • We constructed retroviral vectors to express two different regions of the L-plastin gene, a 1713-bp 3'-coding portion and a 163-bp 5'-untranslated region, both in antisense orientation. (nih.gov)
  • antisense orientation, as observed for the ADAR Alu exon. (psu.edu)
  • YUCCA4 overexpression modulates auxin biosynthesis and transport and influences plant growth and development via crosstalk with abscisic acid in Arabidopsis thaliana Genetics and Molecular Biology, 43, e20190221. (unam.mx)
  • Ty1 is transcribed in both sense and antisense directions. (pubmedcentralcanada.ca)
  • In this model, homology sites of Alu sequences helped duplicating a gene cassette encoding miRNAs, which in turn can target sense Alu sequences and thus alter the fate of free Alu elements. (nih.gov)
  • The study showed that treatment with antisense inhibitors of ICAM-1 significantly prolonged heart transplant survival in mice in a dose-dependent manner and reduced damage to the transplanted heart. (freethesaurus.com)
  • Specific mutation of these two CTCF-binding sites in IGCR1/CBE mutant mice revealed that they function as insulator elements to regulate ordered and lineage-specific V(D)J recombination at the locus (Guo et al. (capecodmushroom.org)
  • At Inserm Unit 1169, Nathalie, Cartier, coordinator of this work, and Patrick Aubourg, director of the unit, proposed the hypothesis that increasing the efflux of cholesterol from the brain by overexpressing CYP46A1 might have a beneficial effect on the elements of Alzheimer pathology. (medicalnewstoday.com)
  • Our results are consistent with an idea that the level of P -element piRNAs is a determinant for dividing strain types between M′ and Q and that the suppression mechanisms of transposable elements, including piRNAs, are varied between natural populations. (biomedcentral.com)
  • Applying genetics and molecular biology to the study of the human pathogen Cryptococcus neoformans. (semanticscholar.org)
  • Thanks were also given to the group of Prof. Tong YP and Li ZS, Institute of Genetics and Developmental Biology, CAS, for their help in mapping the haplotypes in their RIL population. (springer.com)
  • Control of replication of bacterial plasmids: genetics, molecular biology, and physiology of the plasmid R1 system. (asmscience.org)
  • Antisense RNA regulates various systems, such as the REPLICATION of certain PLASMIDS and the transfer of some plasmids by CONJUGATION . (thefreedictionary.com)
  • This work is published in the 10 September 2015 issue of Human Molecular Genetics. (medicalnewstoday.com)
  • 04-02-2015 Jan Raaijmakers (1952) is het nieuwe boegbeeld voor de topsector Life Sciences en Health. (studylib.net)
  • Met ervaring binnen zowel de wetenschap als in het bedrijfsleven, en zijn aandacht voor publiek private samenwerking, is Raaijmakers de aangewezen persoon om innovatie binnen Life Sciences en Health een extra impuls te geven. (studylib.net)
  • Healy J, Corr C, DeYoung J, Baker B (1993) Linked and unlinked transposition of a genetically marked Dissociation element in transgenic tomato. (springer.com)
  • however, the role of zygotic piRNAs derived from paternal P elements is poorly understood. (biomedcentral.com)
  • The amounts of piRNAs produced in ovaries of F1 hybrids varied among the strains and were influenced by the characteristics of the piRNA clusters that harbored the P elements. (biomedcentral.com)
  • However, little is known about the extent to which piRNAs are involved in the P-M hybrid dysgenesis in M′ and Q strains, which show different abilities to regulate the P elements from P strains. (biomedcentral.com)
  • Importantly, the M′ progenies were characterized by a lower abundance of P -element piRNAs in both young ovaries and F1 embryonic bodies. (biomedcentral.com)
  • The Q progenies showed various levels of piRNAs in both young ovaries and F1 embryonic bodies despite all of the Q progenies suppressing P -element transposition in their gonad. (biomedcentral.com)