Antisense Elements (Genetics): Nucleic acids which hybridize to complementary sequences in other target nucleic acids causing the function of the latter to be affected.RNA, Antisense: RNA molecules which hybridize to complementary sequences in either RNA or DNA altering the function of the latter. Endogenous antisense RNAs function as regulators of gene expression by a variety of mechanisms. Synthetic antisense RNAs are used to effect the functioning of specific genes for investigative or therapeutic purposes.Oligonucleotides, Antisense: Short fragments of DNA or RNA that are used to alter the function of target RNAs or DNAs to which they hybridize.DNA, Antisense: DNA that is complementary to the sense strand. (The sense strand has the same sequence as the mRNA transcript. The antisense strand is the template for mRNA synthesis.) Synthetic antisense DNAs are used to hybridize to complementary sequences in target RNAs or DNAs to effect the functioning of specific genes for investigative or therapeutic purposes.Oligodeoxyribonucleotides, Antisense: Short fragments of DNA that are used to alter the function of target RNAs or DNAs to which they hybridize.Genetics: The branch of science concerned with the means and consequences of transmission and generation of the components of biological inheritance. (Stedman, 26th ed)DNA Transposable Elements: Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.Oligoribonucleotides, Antisense: Short fragments of RNA that are used to alter the function of target RNAs or DNAs to which they hybridize.Response Elements: Nucleotide sequences, usually upstream, which are recognized by specific regulatory transcription factors, thereby causing gene response to various regulatory agents. These elements may be found in both promoter and enhancer regions.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Genetics, Population: The discipline studying genetic composition of populations and effects of factors such as GENETIC SELECTION, population size, MUTATION, migration, and GENETIC DRIFT on the frequencies of various GENOTYPES and PHENOTYPES using a variety of GENETIC TECHNIQUES.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Enhancer Elements, Genetic: Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Genetics, Behavioral: The experimental study of the relationship between the genotype of an organism and its behavior. The scope includes the effects of genes on simple sensory processes to complex organization of the nervous system.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Regulatory Sequences, Nucleic Acid: Nucleic acid sequences involved in regulating the expression of genes.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.Trace Elements: A group of chemical elements that are needed in minute quantities for the proper growth, development, and physiology of an organism. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Finite Element Analysis: A computer based method of simulating or analyzing the behavior of structures or components.Elements: Substances that comprise all matter. Each element is made up of atoms that are identical in number of electrons and protons and in nuclear charge, but may differ in mass or number of neutrons.Phenotype: The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.Alu Elements: The Alu sequence family (named for the restriction endonuclease cleavage enzyme Alu I) is the most highly repeated interspersed repeat element in humans (over a million copies). It is derived from the 7SL RNA component of the SIGNAL RECOGNITION PARTICLE and contains an RNA polymerase III promoter. Transposition of this element into coding and regulatory regions of genes is responsible for many heritable diseases.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Models, Genetic: Theoretical representations that simulate the behavior or activity of genetic processes or phenomena. They include the use of mathematical equations, computers, and other electronic equipment.Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Thionucleotides: Nucleotides in which the base moiety is substituted with one or more sulfur atoms.Tumor Cells, Cultured: Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Genetic Variation: Genotypic differences observed among individuals in a population.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Chromosome Mapping: Any method used for determining the location of and relative distances between genes on a chromosome.Long Interspersed Nucleotide Elements: Highly repeated sequences, 6K-8K base pairs in length, which contain RNA polymerase II promoters. They also have an open reading frame that is related to the reverse transcriptase of retroviruses but they do not contain LTRs (long terminal repeats). Copies of the LINE 1 (L1) family form about 15% of the human genome. The jockey elements of Drosophila are LINEs.Regulatory Elements, Transcriptional: Nucleotide sequences of a gene that are involved in the regulation of GENETIC TRANSCRIPTION.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Nucleic Acid Conformation: The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.Repetitive Sequences, Nucleic Acid: Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).Sequence Homology, Nucleic Acid: The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Molecular Biology: A discipline concerned with studying biological phenomena in terms of the chemical and physical interactions of molecules.DNA Primers: Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.RNA, Untranslated: RNA which does not code for protein but has some enzymatic, structural or regulatory function. Although ribosomal RNA (RNA, RIBOSOMAL) and transfer RNA (RNA, TRANSFER) are also untranslated RNAs they are not included in this scope.Transcriptome: The pattern of GENE EXPRESSION at the level of genetic transcription in a specific organism or under specific circumstances in specific cells.Newcastle disease virus: The most well known avian paramyxovirus in the genus AVULAVIRUS and the cause of a highly infectious pneumoencephalitis in fowl. It is also reported to cause CONJUNCTIVITIS in humans. Transmission is by droplet inhalation or ingestion of contaminated water or food.
... antisense elements (genetics) MeSH D27.720.470.530.600.150.200 --- dna, antisense MeSH D27.720.470.530.600.150.200.640 --- ... antisense MeSH D27.720.470.530.600.150.760.645 --- oligoribonucleotides, antisense MeSH D27.720.470.530.600.223 --- dna probes ... antisense MeSH D27.720.470.530.600.150.640.645 --- oligoribonucleotides, antisense MeSH D27.720.470.530.600.150.760 --- rna, ... oligodeoxyribonucleotides, antisense MeSH D27.720.470.530.600.150.640 --- oligonucleotides, antisense MeSH D27.720.470.530. ...
Wagner EG; Altuvia S; Romby P (2002). "Antisense RNAs in bacteria and their genetic elements". Adv Genet. Advances in Genetics ... Antisense RNAs are widespread; most downregulate a gene, but a few are activators of transcription. One way antisense RNA can ... these cis-regulatory elements regulate the activity of that mRNA. The untranslated regions can also contain elements that ... Genetics. 166 (3): 1437-50. doi:10.1534/genetics.166.3.1437. PMC 1470764 . PMID 15082561. Podlevsky JD; Bley CJ; Omana RV; Qi X ...
Saberi F, Kamali M, Najafi A, Yazdanparast A, Moghaddam MM (2016-07-28). "Natural antisense RNAs as mRNA regulatory elements in ... focus on natural antisense transcripts". Trends in Genetics. 28 (8): 389-96. doi:10.1016/j.tig.2012.03.013. PMID 22541732. ... Antisense RNA (asRNA), also referred as antisense transcript, natural antisense transcript (NAT) or antisense oligonucleotide, ... Cis-natural antisense transcript Antisense therapy Pelechano V, Steinmetz LM (December 2013). "Gene regulation by antisense ...
Jurka J. (December 2004). Evolutionary impact of human Alu repetitive elements. Current Opinion in Genetics & Development 14(6 ... The regions coding miRNA can be independent RNA-genes often being anti-sense to neighboring protein-coding genes, or can be ... In fact, the Alu element, a short-interspersed nuclear element of about 300 nucleotides is located at over a million copies ... For this reason, short interspersed nuclear elements are believed to have co-evolved with long interspersed nuclear element ( ...
doi:10.1534/genetics.106.069484. PMC 1894591 . PMID 17409075. Vanhée-Brossollet C, Vaquero C (1998). "Do natural antisense ... It is thought that in malignant cancer cells with activated transposable elements creates a large amount of transcriptional ... The NATsdb or Natural Antisense Transcript database is a rich tool for searching for antisense pairs from multiple organisms. ... Li YY, Qin L, Guo ZM, Liu L, Xu H, Hao P, Su J, Shi Y, He WZ, Li YX (2006). "In silico discovery of human natural antisense ...
... "elements" (enhancers and silencers) on the pre-mRNA transcript itself. These proteins and their respective binding elements ... "Trends in Genetics. 30 (8): 340-347. doi:10.1016/j.tig.2014.05.005. PMC 4112133. PMID 24951248.. ... Splicing events can be experimentally altered[34][35] by binding steric-blocking antisense oligos such as Morpholinos or ... such as by bringing together splicing elements or by masking a sequence that would otherwise serve as a binding element for a ...
Lee JT, Davidow LS, Warshawsky D (April 1999). "Tsix, a gene antisense to Xist at the X-inactivation centre". Nature Genetics. ... "A proximal conserved repeat in the Xist gene is essential as a genomic element for X-inactivation in mouse". Development. 136 ( ... Graves JA (1996). "Mammals that break the rules: genetics of marsupials and monotremes". Annual Review of Genetics. 30: 233-60 ... Nature Reviews Genetics. (10) 155-159. *^ Hoki Y, Kimura N, Kanbayashi M, Amakawa Y, Ohhata T, Sasaki H, Sado T (January 2009 ...
... "elements" (enhancers and silencers) on the pre-mRNA transcript itself. These proteins and their respective binding elements ... Splicing events can be experimentally altered by binding steric-blocking antisense oligos such as Morpholinos or Peptide ... Nature Genetics. 40 (12): 1413-5. doi:10.1038/ng.259. PMID 18978789. Eksi, R; Li, HD; Menon, R; Wen, Y; Omenn, GS; Kretzler, M ... such as by bringing together splicing elements or by masking a sequence that would otherwise serve as a binding element for a ...
... antisense elements (genetics) MeSH D13.444.600.150.200 --- dna, antisense MeSH D13.444.600.150.200.640 --- ... antisense MeSH D13.444.600.150.640.645 --- oligoribonucleotides, antisense MeSH D13.444.600.150.760 --- rna, antisense MeSH ... antisense MeSH D13.150.480.645 --- oligoribonucleotides, antisense MeSH D13.150.650.319 --- micrornas MeSH D13.150.650.640 --- ... antisense MeSH D13.444.308.150.640 --- oligodeoxyribonucleotides, antisense MeSH D13.444.308.180 --- dna, archaeal MeSH D13.444 ...
Alu elements in humans and analogous B1 and B2 elements in mice have succeeded in becoming the most abundant mobile elements ... August 2004). "Antisense intronic non-coding RNA levels correlate to the degree of tumor differentiation in prostate cancer". ... Genetics. 17 (10): 601-614. doi:10.1038/nrg.2016.85. ISSN 1471-0064. PMID 27573374. Johnsson, Per; Lipovich, Leonard; Grandér, ... The abundance and distribution of Alu elements and similar repetitive elements throughout the mammalian genome may be partly ...
Rodriguez-Jato S, Nicholls RD, Driscoll DJ, Yang TP (2005). "Characterization of cis- and trans-acting elements in the ... Human Molecular Genetics. 5 (4): 517-24. doi:10.1093/hmg/5.4.517. PMID 8845846. Poukka H, Aarnisalo P, Santti H, Jänne OA, ... "The IC-SNURF-SNRPN transcript serves as a host for multiple small nucleolar RNA species and as an antisense RNA for UBE3A". ... Human Molecular Genetics. 10 (23): 2687-700. doi:10.1093/hmg/10.23.2687. PMID 11726556. Runte M, Kroisel PM, Gillessen-Kaesbach ...
Graves JA (1996). "Mammals that break the rules: genetics of marsupials and monotremes". Annual Review of Genetics. 30: 233-260 ... The Tsix RNA is transcribed antisense to Xist, meaning that the Tsix gene overlaps the Xist gene and is transcribed on the ... "A proximal conserved repeat in the Xist gene is essential as a genomic element for X-inactivation in mouse". Development. 136 ( ... Nature Reviews Genetics. (10) 155-159. Ng K, Pullirsch D, Leeb M, Wutz A (2007). "Xist and the order of silencing" (Review ...
Airn is the antisense gene of Ifg2r, so if Airn is being transcribed, the transcriptional machinery may cover a part of or the ... Trans acting elements, however, are gene products from one location that act on a different chromosome, either the other in a ... "Russel Silver Syndrome." Genetics Home Reference. US National Library of Medicine, Apr. 2008. Web. 09 May 2015. "Prader Willi ... Recent research has shown that a repeat element in the RNA of Xist causes PRC2 to bind to the RNA. Another part of the RNA ...
... including cancer genetics research, phylogenetic research, and human genetics. Irrespective of the approach used to amplify the ... The loci of Alu element insertion is selected, amplified and evaluated in terms of size of the fragment. The sex assay utilizes ... and two for the antisense strand. The primers bind adjacent to one another, forming a segment of double stranded DNA that once ... Nature Genetics. 6 (2): 130-5. doi:10.1038/ng0294-130. PMID 8162066. Rui, Lixin; Emre, N.C. Tolga; Kruhlak, Michael J.; Chung, ...
"A simple method for delivering Morpholino antisense oligos into the cytoplasm of cells". Antisense Nucleic Acid Drug Dev. 6 (3 ... Nasevicius, A; Ekker SC (2000). "Effective targeted gene 'knockdown' in zebrafish". Nature Genetics. 26 (2): 216-20. doi: ... "Correction of aberrant FGFR1 alternative RNA splicing through targeting of intronic regulatory elements". Hum Mol Genet. 13 (20 ... Penn, AC; Balik A; Greger IH (2012). "Steric antisense inhibition of AMPA receptor Q/R editing reveals tight coupling to ...
Pierce (2005). GENETICS: A Conceptual Approach. Weiss, B., ed. (1997). Antisense Oligodeoxynucleotides and Antisense RNA : ... Oligonucleotides are also indispensable elements in antisense therapy. Oligonucleotides composed of 2'-deoxyribonucleotides ( ... In the case of antisense RNA they prevent protein translation of certain messenger RNA strands by binding to them. Antisense ... 1997). Antisense Oligodeoxynucleotides and Antisense RNA : Novel Pharmacological and Therapeutic Agents. Boca Raton, FL: CRC ...
This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding ... Tortora G, Clair T, Cho-Chung YS (Jan 1990). "An antisense oligodeoxynucleotide targeted against the type II beta regulatory ... Cytogenetics and Cell Genetics. 45 (3-4): 237-9. doi:10.1159/000132461. PMID 3691190. Budillon A, Cereseto A, Kondrashin A, ... Luo Z, Singh IS, Fujihira T, Erlichman J (Dec 1992). "Characterization of a minimal promoter element required for transcription ...
Antisense Antisense RNA Antisense strand Antisense therapy AP endonuclease AP site Apert syndrome Apoptosis Applied genetics ... site P element P1 P53 Palindrome Panmictic Paralogous genes Paramecin Parameters Parental Parental ditype Parental imprinting ... Genetic variation Geneticist Genetics Genetics and Archaeogenetics of South Asia Genetics experiments Genic balance theory ... 2000), Chapter 1 (Genetics and the Organism): Introduction List of genetics research organizations List of geneticists & ...
Genes ratA and txpA are found within a 48kb phage-like element called skin. This element interrupts a gene for the sigma factor ... Genetics. 172 (2): 1309-23. doi:10.1534/genetics.105.042895. PMC 1456228 . PMID 16299387. Retrieved 2010-08-13. Page for RNA ... Lee JM, Zhang S, Saha S, Santa Anna S, Jiang C, Perkins J (December 2001). "RNA expression analysis using an antisense Bacillus ... The toxin-antitoxin system contained within skin forces the inheritance of this element, which is acting as a selfish gene. The ...
The MYCN gene has an antisense RNA, N-cym or MYCNOS, transcribed from the opposite strand which can be translated to form a ... "Differential effects of the widely expressed dMax splice variant of Max on E-box vs initiator element-mediated regulation by c- ... PLoS Genetics. 10 (1): e1003996. doi:10.1371/journal.pgen.1003996. PMC 3879166 . PMID 24391509. Cheng JM, Hiemstra JL, ... It has been shown that NCYM antisense RNA encodes for a protein that has originated de novo and is specific to human and ...
Production of antisense Q RNA shuts off Q production, reducing lytic activity until the repressor cI can adequately shut off ... It serves as the switch element for establishing repression of the lytic genes after infection, producing the lysogenic ... Genetics. 149: 1633-1648. PMC 1460268 . PMID 9691025. Rattray, A.; Altuvia, A.; Mahajna, G.; Oppenheim, A.B.; Gottesman, M. ( ... Krinke, L.; Wulff, D.L. (1990). "RNase III-dependent hydrolysis of λcII-O gene mRNA mediated by λ OOP antisense RNA". Genes Dev ...
Alu repetitive element is member of Short Interspersed Elements (SINE) in mammalian genome. There are about 300 to 500 thousand ... There are three method are developed for this purpose: gene replacement, sense and anti-sense suppression, and insertional ... "Facilitating genome navigation: survey sequencing and dense radiation-hybrid gene mapping." Nature Reviews Genetics 6.8 (2005 ... However, using transposable element can bring more advantages. Transposable elements were first discovered by Barbara ...
Just two P elements in the telomere are enough to suppress over 80 other copies of the P element in the genome. The cytoplasmic ... Most piRNAs are antisense to mRNAs transcribed from the silenced transposons, generally associating with Piwi and Aubergine ( ... "Telomeric Trans-Silencing: An Epigenetic Repression Combining RNA Silencing and Heterochromatin Formation". PLoS Genetics. 3: ... The P elements have an extremely high transposition rate and induce sterility and abnormal gonad development in D. melanogaster ...
Long INterspersed Elements (LINE) are a group of genetic elements that are found in large numbers in eukaryotic genomes, ... Liang, Kung-Hao; Yeh, Chau-Ting (2013). "A gene expression restriction network mediated by sense and antisense Alu sequences ... Stansfield, William D.; King, Robert C. (1997). A dictionary of genetics (5th ed.). Oxford [Oxfordshire]: Oxford University ... Non-LTR retrotransposons consist of two sub-types, long interspersed elements (LINEs) and short interspersed elements (SINEs). ...
RNAi is now known as precise, efficient, stable and better than antisense therapy for gene suppression.[2] However, antisense ... "Nature Genetics. 36 (11): 1174-80. doi:10.1038/ng1452. PMID 15475954.. *^ Sugiyama T, Cam H, Verdel A, Moazed D, Grewal SI ( ... The 3'-UTR often contains microRNA response elements (MREs). MREs are sequences to which miRNAs bind. These are prevalent ... "Genetics and Molecular Biology. 34 (3): 363-70. doi:10.1590/S1415-47572011000300001. PMC 3168173. PMID 21931505.. ...
If one strand of the double-stranded DNA is considered the sense strand, then the other strand, considered the antisense strand ... of box ACA small nucleolar RNAs is defined by an evolutionarily conserved secondary structure and ubiquitous sequence elements ... "Genetics Home Reference. 16 March 2015.. *^ "Genetic Testing". nih.gov.. *^ "Definitions of Genetic Testing". Definitions of ... "antisense" sequence which is by itself nonfunctional, but can bind to the sense strand. ...
Antisense Elements (Genetics). Known as: Antisense Elements (Genetics) [Chemical/Ingredient], Anti-Sense Elements, Elements, ... Identification of antisense nucleic acid hybridization sites in mRNA molecules with self-quenching fluorescent reporter ... Nuclease resistance and RNA affinity are key criteria in the search for optimal antisense nucleic acid modifications, but the… ... HPV E6 antisense induces apoptosis in CaSki cells via suppression of E6 splicing ...
Antigens, CD80 / genetics, immunology*. Antisense Elements (Genetics)*. Down-Regulation. Gene Transfer Techniques. Graft ... RESULTS: Antisense gene transfer resulted in markedly downregulation of B7.1 in the donor livers, attenuated acute immune ... CONCLUSIONS: Blocking expression of B7.1 in liver by antisense gene therapy may represent a potential strategy to attenuate ... METHODS: The liver grafts from 60 Dark Agouti rats were intraportally perfused with antisense B7.1 expression vector, before ...
Research Progress in Antisense Elements (Genetics). New York: Nova Biomedical Books, 2008, pp.87-123. ... Zinad HS, Natasya I, Werner A. Natural antisense transcripts at the interface between host genome and mobile genetic elements. ... Natural antisense transcripts (NATs). Large proportions of the human genome do not code for proteins and were long considered ... Translated anti-sense product of the Na/phosphate co- transporter (NaPi-II). Biochemical Journal 1998, 332, 483-489. ...
1999). "Cosuppression of I transposon activity in Drosophila by I-containing sense and antisense transgenes." Genetics 153(4): ... Another elements occurs to cause other types of Hybrid Dysgenesis. One of them is called P element, which are DNA transposons [ ... 1996). "Repression of hybrid dysgenesis in Drosophila melanogaster by heat-shock-inducible sense and antisense P-element ... High level of transposible element activity drives cell to death [85]. P element is under the control of RNA mediated mechanism ...
... active and acute lesions were studied for X2 MBP expression by in situ hybridization utilizing a synthetic oligonucleotide anti-sense ... active and acute lesions were studied for X2 MBP expression by in situ hybridization utilizing a synthetic oligonucleotide anti-sense ... active and acute lesions were studied for X2 MBP expression by in situ hybridization utilizing a synthetic oligonucleotide anti-sense ... active and acute lesions were studied for X2 MBP expression by in situ hybridization utilizing a synthetic oligonucleotide anti-sense ...
Both 35S-labeled RNA antisense and sense probes to amyloid β protein messenger RNA were used to ensure specific hybridization. ... Both 35S-labeled RNA antisense and sense probes to amyloid β protein messenger RNA were used to ensure specific hybridization. ... Both 35S-labeled RNA antisense and sense probes to amyloid β protein messenger RNA were used to ensure specific hybridization. ... Both 35S-labeled RNA antisense and sense probes to amyloid β protein messenger RNA were used to ensure specific hybridization. ...
... antisense elements (genetics) MeSH D27.720.470.530.600.150.200 --- dna, antisense MeSH D27.720.470.530.600.150.200.640 --- ... antisense MeSH D27.720.470.530.600.150.760.645 --- oligoribonucleotides, antisense MeSH D27.720.470.530.600.223 --- dna probes ... antisense MeSH D27.720.470.530.600.150.640.645 --- oligoribonucleotides, antisense MeSH D27.720.470.530.600.150.760 --- rna, ... oligodeoxyribonucleotides, antisense MeSH D27.720.470.530.600.150.640 --- oligonucleotides, antisense MeSH D27.720.470.530. ...
It is suggested that these abilities may be mediated by P-encoded polypeptides or by antisense P RNAs initiated from external ... Three of the elements were 1.1-kb KP elements, one was a 1.2-kb element called D50, and one was a 0.5-kb element called SP. ... the SP element repressed snw mutability only when the transposase came from complete P elements and the D50 element repressed ... Gonadal dysgenesis was repressed by two of the KP elements (denoted KP(1) and KP(6)) and by SP, but not by the third KP element ...
Genetics. 1995;139:81-94. [PubMed]. 73. Petrov DA, Schutzman JL, Hartl DL, Lozovskaya ER. Diverse transposable elements are ... 1. Biemont C. A brief history of the status of transposable elements: from junk DNA to major players in evolution. Genetics. ... Positive and negative regulatory elements control expression of the yeast retrotransposon Ty3. Genetics. 1993;134:685-700. [ ... at the Ty1-DR3 element, in bas1Δ and bas1Δ tye7Δ cells. We previously showed that the 5′LTR of several endogenous Ty1 elements ...
Antisense Elements (Genetics)/genetics*. *Antisense Elements (Genetics)/pharmacology*. *Carcinoma/genetics. *Carcinoma/ ... The antisense construct for the 5-untranslated region with the most unique sequence for the L-plastin gene was more effective ... Suppression of prostate carcinoma cell invasion by expression of antisense L-plastin gene.. Zheng J1, Rudra-Ganguly N, Powell ... Cells infected with the 163-bp antisense virus, which were also tested in a nude mouse diaphragm invasion model, showed ...
Antisense Elements (Genetics). *Base Sequence. *Basement Membrane/chemistry. *Basement Membrane/embryology*. *Basement Membrane ...
Antisense Elements (Genetics)/pharmacology. *Cells, Cultured. *Chromosome Mapping. *Chromosomes, Artificial, Bacterial. *Gene ... In addition, morpholino-based antisense inhibition of Naip5 causes an increase in the Legionella permissiveness of macrophages. ...
Antisense Elements (Genetics)/pharmacology. *Base Sequence. *Blood Proteins/drug effects. *Blood Proteins/genetics ... Antisense oligonucleotides to hBD-1 ablated the antimicrobial activity in airway surface fluid from non-CF grafts. These data ...
Comprehensive expressional analyses of antisense transcripts in colon cancer tissues using artificial antisense probes. Saito, ... Hirose, Y., Ikeda, K. T., Noro, E., Hiraoka, K., Tomita, M. & Kanai, A., 2015 7 14, : : BMC Genetics. 16, 1, 83.. 研究成果: Article ... Tamaki, S., Tomita, M., Suzuki, H. & Kanai, A., 2018 1 8, : : Frontiers in Genetics. 8, JAN, 227.. 研究成果: Article ... Comparative analysis of cis-encoded antisense RNAs in eukaryotes. Numata, K., Okada, Y., Saito, R., Kiyosawa, H., Kanai, A. & ...
Antisense probes containing 2-aminoadenosine allow efficient depletion of U5 snRNP from HeLa splicing extracts. Lamm, G. M., ...
Adenoviridae , Genetics , Animals , Antisense Elements (Genetics) , Cell Line , Cell Proliferation , Genes, myc , Genetics , ... Adenoviridae , Genetics , Animals , Antisense Elements (Genetics) , Collagen Type I , DNA-Binding Proteins , Genetics , Liver ... Antisense Elements (Genetics) , Genetics , Bone Neoplasms , Genetics , Metabolism , Pathology , Cell Division , Humans , ... Animals , Antisense Elements (Genetics) , Genetics , Apoptosis , Cell Hypoxia , Cells, Cultured , Female , Male , Mitogen- ...
Antisense Elements (Genetics). *Oligonucleotide Probes. *Protein Isoforms. *Transforming Growth Factor beta. Grant support. * ...
Wagner EG; Altuvia S; Romby P (2002). "Antisense RNAs in bacteria and their genetic elements". Adv Genet. Advances in Genetics ... Antisense RNAs are widespread; most downregulate a gene, but a few are activators of transcription. One way antisense RNA can ... these cis-regulatory elements regulate the activity of that mRNA. The untranslated regions can also contain elements that ... Genetics. 166 (3): 1437-50. doi:10.1534/genetics.166.3.1437. PMC 1470764 . PMID 15082561. Podlevsky JD; Bley CJ; Omana RV; Qi X ...
Antisense Elements (Genetics) Medicine & Life Sciences * RNA Probes Medicine & Life Sciences * Sexual Development Medicine & ... It utilizes a 32P-labeled antisense RNA probe that is first hybridized in solution to tissue mRNA and then subjected to ... It utilizes a 32P-labeled antisense RNA probe that is first hybridized in solution to tissue mRNA and then subjected to ... It utilizes a 32P-labeled antisense RNA probe that is first hybridized in solution to tissue mRNA and then subjected to ...
RNA, Messenger/genetics. Substances. *Antisense Elements (Genetics). *MYCN protein, human. *N-Myc Proto-Oncogene Protein ... NCYM, a Cis-antisense gene of MYCN, encodes a de novo evolved protein that inhibits GSK3β resulting in the stabilization of ... NCYM, a Cis-Antisense Gene of MYCN, Encodes a De Novo Evolved Protein That Inhibits GSK3β Resulting in the Stabilization of ... NCYM, a Cis-Antisense Gene of MYCN, Encodes a De Novo Evolved Protein That Inhibits GSK3β Resulting in the Stabilization of ...
An analysis of 103906 Alu elements across 6 human chromosomes was carried out, using the presence of orthologous Alu elements ... These observations have been made by relying on the subfamily as a proxy for age of an element. In this study, we suggest that ... Instead, elements are preferentially lost from areas of high GC content over time. The correlation between GC content and ... Alu elements are found in low GC regions and old Alus in high GC regions. The correlation between high GC regions and high ...
RNA, Plant/genetics. Substances. *APETALA2 protein, Arabidopsis. *Antisense Elements (Genetics). *Arabidopsis Proteins ... In situ hybridization using an anti-sense [(F) and (H)] or sense [(G) and (I)] miRNA172 probe on longitudinal sections of ...
In contrast, nuclear retention is promoted by specialized cis-elements found in certain RNAs. This new understanding of the ... In contrast, nuclear retention is promoted by specialized cis-elements found in certain RNAs. This new understanding of the ... As described above, if a pair of transposable elements are found in the sense and anti-sense orientation in a single transcript ... Other cis-Elements That Promote Nuclear Retention. A few other cis-elements that promote nuclear retention have been ...
Antisense Elements (Genetics). *Apolipoprotein B-100 Synthesis Inhibitor. *Carbohydrates. *Compounds used in a research, ... Potent reduction of apolipoprotein B and low-density lipoprotein cholesterol by short-term administration of an antisense ...
Here we searched for natural antisense transcript for sensorin (NAT-SRN), a neuropeptide expressed in the presynaptic sensory ... These studies reveal novel insights into physiological regulation of natural antisense RNAs. ... These studies reveal novel insights into physiological regulation of natural antisense RNAs. ... Here we searched for natural antisense transcript for sensorin (NAT-SRN), a neuropeptide expressed in the presynaptic sensory ...
  • The antisense construct for the 5'-untranslated region with the most unique sequence for the L-plastin gene was more effective in down-regulation efficiency compared with the larger antisense construct in the coding region, which maintains homology to other members of the plastin gene family. (nih.gov)
  • For example, the 5' of the Alu sine is derived from 7SL RNA, a sequence transcribed by RNA Polymerase III which codes for the RNA element of SRP, an abundant ribonucleoprotein. (wikipedia.org)
  • Incomplete P elements do not produce the transposase because they lack part of the coding sequence. (genetics.org)
  • This yielded all Alu elements without and with an overlap of at least one expressed sequence tag (EST), in the case of the intronic and exonic data sets, respectively. (psu.edu)
  • An analysis of C. elegans transposable elements =-=[25,=- revealed that a 205-bp ITR sequence within Turmoil-1 is highly similar to a region of two exons separated by an intron of the rsp-2 gene (see pairwise alignment using bl2seq Figure 1B). (psu.edu)
  • Another, the DRE/TGE element of the tra-2 3′ UTR, was not detected by sequence analysis. (genetics.org)
  • However, it is noted that the specific sequence of intronic splicing elements and the number of nucleotides between the branchpoint and the nearest 3' acceptor site affect splice site selection. (wikipedia.org)
  • The sequence at -10 is called the [[Pribnow box]], or the -10 element, and usually consists of the six nucleotides TATAAT. (wikidoc.org)
  • The other sequence at -35 (the -35 element) usually consists of the six nucleotides TTGACA. (wikidoc.org)
  • Initial studies into lncRNA conservation noted that as a class, they were enriched for conserved sequence elements, depleted in substitution and insertion/deletion rates and depleted in rare frequency variants, indicative of purifying selection maintaining lncRNA function. (wikipedia.org)
  • We refer to the repeat as the Correia element (CE), but it has also been known as NEMIS ( Neisseria miniature insertion sequence) and CREE (Correia repeat enclosed element). (prolekare.cz)
  • Their work demonstrated that additional sequence elements of specific miRNAs can control their posttranscriptional behavior, including the subcellular localization. (prolekare.cz)
  • In 2003, piRNAs derived largely from repeated sequence elements, including transposons, were found in abundance in male and female Drosophila germlines (Theurkauf, 5). (wikipedia.org)
  • The snoRNA molecule contains an antisense element (a stretch of 10-20 nucleotides), which are base complementary to the sequence surrounding the base (nucleotide) targeted for modification in the pre-RNA molecule. (wikipedia.org)
  • These families of snoRNAs are referred to as antisense C/D box and H/ACA box snoRNAs based on the presence of conserved sequence motifs in the snoRNA. (wikipedia.org)
  • Many piRNAs are derived from transposons and other repeated elements, and therefore lack specific loci. (wikipedia.org)
  • The cytoplasmic factor used for TSE builds up over generations and suppression of the transposons is not fully effective unless the fly's female-line ancestors have had the P element for six generations (2). (wikipedia.org)
  • Polymorphisms for Alu insertions have been much studied as a tool in human population genetic inference, particularly because the absence of the element can always be identified as the ancestral state ( Batzer & Deininger, 2002 ). (peerj.com)
  • We have introduced a genetically marked Dissociation transposable element ( Ds HPT ) into tomato ( Lycopersicon esculentum ) by Agrobacterium tumefaciens -mediated transformation. (springer.com)
  • Chuck G, Robbins T, Nijjar C, Ralston E, Courtney-Gutterson N, Dooner HK (1993) Tagging and cloning of a petunia flower color gene with the maize transposable element Activator . (springer.com)
  • Greenblatt IM (1984) A chromosomal replication pattern deduced from pericarp phenotypes resulting from movements of the transposable element Modulator in maize. (springer.com)
  • One of the most abundant repeats is a miniature inverted-repeat transposable-element (MITE) first identified by Correia and colleagues in 1986 , . (prolekare.cz)
  • Transposable element insertions have been linked to many diseases including hemophilia, severe combined immunodeficiency, and predisposition to cancer. (wikipedia.org)
  • The idea that asRNAs as drug targets started in 1978 when Zamecnik and Stephenson found an antisense oligoneucleotides to the viral RNA of Rous scarcoma virus was capable of inhibiting viral replication and protein synthesis. (wikipedia.org)
  • NCYM, a Cis-antisense gene of MYCN, encodes a de novo evolved protein that inhibits GSK3β resulting in the stabilization of MYCN in human neuroblas. (nih.gov)
  • Here we show that NCYM, a cis-antisense gene of the MYCN oncogene, initially thought to be a large non-coding RNA, encodes a de novo evolved protein regulating the pathogenesis of human cancers, particularly neuroblastoma. (nih.gov)
  • It has been shown that NCYM antisense RNA encodes for a protein that has originated de novo and is specific to human and chimpanzee. (wikipedia.org)
  • Gary Bruce Ruvkun (born 26 March 1952, Berkeley, California) is an American molecular biologist at Massachusetts General Hospital and professor of genetics at Harvard Medical School in Boston. (wikipedia.org)
  • however, the role of zygotic piRNAs derived from paternal P elements is poorly understood. (biomedcentral.com)
  • The amounts of piRNAs produced in ovaries of F1 hybrids varied among the strains and were influenced by the characteristics of the piRNA clusters that harbored the P elements. (biomedcentral.com)
  • However, little is known about the extent to which piRNAs are involved in the P-M hybrid dysgenesis in M′ and Q strains, which show different abilities to regulate the P elements from P strains. (biomedcentral.com)
  • Importantly, the M′ progenies were characterized by a lower abundance of P -element piRNAs in both young ovaries and F1 embryonic bodies. (biomedcentral.com)
  • The Q progenies showed various levels of piRNAs in both young ovaries and F1 embryonic bodies despite all of the Q progenies suppressing P -element transposition in their gonad. (biomedcentral.com)
  • In contrast, when P -strain females are mated with P-strain males, P- element mobilization is prevented by maternally deposited piRNAs in the germline cells and early embryos, which are laid by P-strain mothers but not P-progeny mothers (referred to as F1 embryos of P progenies) [ 21 ]. (biomedcentral.com)
  • Regulation of the cohesin-loading factor NIPBL: Role of the lncRNA NIPBL-AS1 and identification of a distal enhancer element. (nih.gov)
  • Regulation by TP5 , a structurally incomplete P element at the X telomere, is more profoundly impaired than regulation by TP6 , a different incomplete P element inserted at the same site in a TAS repeat at the X telomere. (genetics.org)
  • Regulation by the telomeric P elements therefore exactly parallels regulation by the P cytotype. (genetics.org)
  • He was a founding member of the Basic Sciences Division, and professor of genetics at UW. (wikipedia.org)
  • Morpholinos do not trigger the degradation of their target RNA molecules, unlike many antisense structural types (e.g., phosphorothioates, siRNA). (wikipedia.org)