Antimicrobial Cationic Peptides: Small cationic peptides that are an important component, in most species, of early innate and induced defenses against invading microbes. In animals they are found on mucosal surfaces, within phagocytic granules, and on the surface of the body. They are also found in insects and plants. Among others, this group includes the DEFENSINS, protegrins, tachyplesins, and thionins. They displace DIVALENT CATIONS from phosphate groups of MEMBRANE LIPIDS leading to disruption of the membrane.Microscopy, Atomic Force: A type of scanning probe microscopy in which a probe systematically rides across the surface of a sample being scanned in a raster pattern. The vertical position is recorded as a spring attached to the probe rises and falls in response to peaks and valleys on the surface. These deflections produce a topographic map of the sample.Nanotechnology: The development and use of techniques to study physical phenomena and construct structures in the nanoscale size range or smaller.beta-Defensins: DEFENSINS found mainly in epithelial cells.Cathelicidins: Antimicrobial cationic peptides with a highly conserved amino terminal cathelin-like domain and a more variable carboxy terminal domain. They are initially synthesized as preproproteins and then cleaved. They are expressed in many tissues of humans and localized to EPITHELIAL CELLS. They kill nonviral pathogens by forming pores in membranes.Anti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.Anti-Infective Agents: Substances that prevent infectious agents or organisms from spreading or kill infectious agents in order to prevent the spread of infection.Bacterial Proteins: Proteins found in any species of bacterium.Peptides: Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.Polymyxin B: A mixture of polymyxins B1 and B2, obtained from Bacillus polymyxa strains. They are basic polypeptides of about eight amino acids and have cationic detergent action on cell membranes. Polymyxin B is used for infections with gram-negative organisms, but may be neurotoxic and nephrotoxic.Styrax: A plant genus of the family STYRACACEAE. Sap of these Asian trees are a source of a balsam (BALSAMS). This styrax balsam is 3/4 coniferyl benzoate, 1/8 free BENZOIC ACID, along with benzyl cinnamate, vanillin, and TRITERPENES.ArchivesBiological Science Disciplines: All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Directories as Topic: Lists of persons or organizations, systematically arranged, usually in alphabetic or classed order, giving address, affiliations, etc., for individuals, and giving address, officers, functions, and similar data for organizations. (ALA Glossary of Library and Information Science, 1983)Polylysine: A peptide which is a homopolymer of lysine.Publishing: "The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.Serial Publications: Publications in any medium issued in successive parts bearing numerical or chronological designations and intended to be continued indefinitely. (ALA Glossary of Library and Information Science, 1983, p203)Melitten: Basic polypeptide from the venom of the honey bee (Apis mellifera). It contains 26 amino acids, has cytolytic properties, causes contracture of muscle, releases histamine, and disrupts surface tension, probably due to lysis of cell and mitochondrial membranes.Cell Membrane Permeability: A quality of cell membranes which permits the passage of solvents and solutes into and out of cells.Pseudomonas aeruginosa: A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection.Cecropins: Antimicrobial peptides that form channels in membranes that are more permeable to anions than cations. They resemble MAGAININS, with their N-terminal region forming a positively charged amphipathic alpha helix, but containing an additional C-terminal segment.Lipopolysaccharides: Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed)Acrylonitrile: A highly poisonous compound used widely in the manufacture of plastics, adhesives and synthetic rubber.Enterobacter cloacae: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria that occurs in water, sewage, soil, meat, hospital environments, and on the skin and in the intestinal tract of man and animals as a commensal.

Range of activity and metabolic stability of synthetic antibacterial glycopeptides from insects. (1/2987)

Antibacterial glycopeptides isolated from insects are exciting bio-oligomers because they represent a family of compounds in which the structural and functional effects of incorporating short O-linked sugars to protein fragments can be studied. Additionally, their high activity in vitro warrants detailed further drug development efforts. Due to the limited availability of the isolated material, we used synthetic glycopeptides and some analogs to investigate the range of activity of drosocin and pyrrhocoricin. While addition of the Gal-GalNAc disaccharide to the natural mid-chain position generally increased the antibacterial activity of drosocin, pyrrhocoricin lacking sugar appeared to be more potent, with an IC50 against Escherichia coli D22 of 150 nM. Although glycosylated drosocin was active against E. coli in the low microM range in vitro, this peptide was completely inactive when injected into mice. The lack of in vivo activity of drosocin could be explained by the unusually high degradation rate of the peptides in mammalian sera. The early degradation products were inactive in vitro. In contrast, the peptides were considerably more stable in insect hemolymph, where their natural activity is manifested.  (+info)

Biological properties of structurally related alpha-helical cationic antimicrobial peptides. (2/2987)

A series of alpha-helical cationic antimicrobial peptide variants with small amino acid changes was designed. Alterations in the charge, hydrophobicity, or length of the variant peptides did not improve the antimicrobial activity, and there was no statistically significant correlation between any of these factors and the MIC for Pseudomonas aeruginosa, Escherichia coli, or Salmonella typhimurium. Individual peptides demonstrated synergy with conventional antibiotics against antibiotic-resistant strains of P. aeruginosa. The peptides varied considerably in the ability to bind E. coli O111:B4 lipopolysaccharide (LPS), and this correlated significantly with their antimicrobial activity and ability to block LPS-stimulated tumor necrosis factor and interleukin-6 production. In general, the peptides studied here demonstrated a broad range of activities, including antimicrobial, antiendotoxin, and enhancer activities.  (+info)

Inactivation of the dlt operon in Staphylococcus aureus confers sensitivity to defensins, protegrins, and other antimicrobial peptides. (3/2987)

Positively charged antimicrobial peptides with membrane-damaging activity are produced by animals and humans as components of their innate immunity against bacterial infections and also by many bacteria to inhibit competing microorganisms. Staphylococcus aureus and Staphylococcus xylosus, which tolerate high concentrations of several antimicrobial peptides, were mutagenized to identify genes responsible for this insensitivity. Several mutants with increased sensitivity were obtained, which exhibited an altered structure of teichoic acids, major components of the Gram-positive cell wall. The mutant teichoic acids lacked D-alanine, as a result of which the cells carried an increased negative surface charge. The mutant cells bound fewer anionic, but more positively charged proteins. They were sensitive to human defensin HNP1-3, animal-derived protegrins, tachyplesins, and magainin II, and to the bacteria-derived peptides gallidermin and nisin. The mutated genes shared sequence similarity with the dlt genes involved in the transfer of D-alanine into teichoic acids from other Gram-positive bacteria. Wild-type strains bearing additional copies of the dlt operon produced teichoic acids with higher amounts of D-alanine esters, bound cationic proteins less effectively and were less sensitive to antimicrobial peptides. We propose a role of the D-alanine-esterified teichoic acids which occur in many pathogenic bacteria in the protection against human and animal defense systems.  (+info)

Recombinant bactericidal/permeability-increasing protein (rBPI21) in combination with sulfadiazine is active against Toxoplasma gondii. (4/2987)

The activity of recombinant bactericidal/permeability-increasing protein (rBPI21), alone or in combination with sulfadiazine, on the intracellular replication of Toxoplasma gondii was assessed in vitro and in mice with acute toxoplasmosis. rBPI21 markedly inhibited the intracellular growth of T. gondii in human foreskin fibroblasts (HFFs). Following 72 h of exposure, the 50% inhibitory concentration of rBPI21 for T. gondii was 2.6 micrograms/ml, whereas only slight cytotoxicity for HFF cells was observed at the concentrations tested. Subsequent mathematical analyses revealed that the combination of rBPI21 with sulfadiazine yielded slight to moderate synergistic effects against T. gondii in vitro. Infection of mice orally with C56 cysts or intraperitoneally (i.p.) with RH tachyzoites resulted in 100% mortality, whereas prolongation of the time to death or significant survival (P = 0.002) was noted for those animals treated with 5 to 20 mg of rBPI21 per kg of body weight per day. Treatment with rBPI21 in combination with sulfadiazine resulted in significant (P = 0.0001) survival of mice infected i.p. with tachyzoites but not of mice infected orally with T. gondii cysts. These results indicate that rBPI21 is active in vitro and in vivo against T. gondii and that its activity is significantly enhanced when it is used in combination with sulfadiazine. To our knowledge, this is the first report of the activity of rBPI21 against a protozoan parasite.  (+info)

In vitro antibacterial properties of pexiganan, an analog of magainin. (5/2987)

Pexiganan, a 22-amino-acid antimicrobial peptide, is an analog of the magainin peptides isolated from the skin of the African clawed frog. Pexiganan exhibited in vitro broad-spectrum antibacterial activity when it was tested against 3,109 clinical isolates of gram-positive and gram-negative, anaerobic and aerobic bacteria. The pexiganan MIC at which 90% of isolates are inhibited (MIC90) was 32 micrograms/ml or less for Staphylococcus spp., Streptococcus spp., Enterococcus faecium, Corynebacterium spp., Pseudomonas spp., Acinetobacter spp., Stenotrophomonas spp., certain species of the family Enterobacteriaceae, Bacteroides spp., Peptostreptococcus spp., and Propionibacterium spp. Comparison of the MICs and minimum bactericidal concentrations (MBCs) of pexiganan for 143 isolates representing 32 species demonstrated that for 92% of the isolates tested, MBCs were the same or within 1 twofold difference of the MICs, consistent with a bactericidal mechanism of action. Killing curve analysis showed that pexiganan killed Pseudomonas aeruginosa rapidly, with 10(6) organisms/ml eliminated within 20 min of treatment with 16 micrograms of pexiganan per ml. No evidence of cross-resistance to a number of other antibiotic classes was observed, as determined by the equivalence of the MIC50s and the MIC90s of pexiganan for strains resistant to oxacillin, cefazolin, cefoxitin, imipenem, ofloxacin, ciprofloxacin, gentamicin, and clindamicin versus those for strains susceptible to these antimicrobial agents. Attempts to generate resistance in several bacterial species through repeated passage with subinhibitory concentrations of pexiganan were unsuccessful. In conclusion, pexiganan exhibits properties in vitro which make it an attractive candidate for development as a topical antimicrobial agent.  (+info)

A gene encoding a hevein-like protein from elderberry fruits is homologous to PR-4 and class V chitinase genes. (6/2987)

We isolated SN-HLPf (Sambucus nigra hevein-like fruit protein), a hevein-like chitin-binding protein, from mature elderberry fruits. Cloning of the corresponding gene demonstrated that SN-HLPf is synthesized as a chimeric precursor consisting of an N-terminal chitin-binding domain corresponding to the mature elderberry protein and an unrelated C-terminal domain. Sequence comparisons indicated that the N-terminal domain of this precursor has high sequence similarity with the N-terminal domain of class I PR-4 (pathogenesis-related) proteins, whereas the C terminus is most closely related to that of class V chitinases. On the basis of these sequence homologies the gene encoding SN-HLPf can be considered a hybrid between a PR-4 and a class V chitinase gene.  (+info)

Endocytosis of heparin-binding protein (CAP37) is essential for the enhancement of lipopolysaccharide-induced TNF-alpha production in human monocytes. (7/2987)

Heparin-binding protein (HBP), also known as CAP37, is a proteolytically inactive serine protease homologue that is released from activated granulocytes. However, HBP is not a biologically inactive molecule but rather a multifunctional protein with properties that include the enhancement of LPS-induced TNF-alpha production from monocytes. We have previously demonstrated that HBP is internalized in monocytes. In the current study, we hypothesize that HBP is internalized in monocytes via endocytosis, and this internalization is an important mechanism by which HBP enhances LPS-induced TNF-alpha release. Using whole blood from healthy donors and flow cytometry, we found that colchicine (0.1-10 mM), cytochalasin D (1000 microM), NH4Cl (10-50 mM), and bafilomycin A1 (0.1-3 microM) significantly reduced the affinity of FITC-HBP for CD14-positive monocytes. Using isolated human monocytes and ELISA, we found that colchicine (0.1 mM), cytochalasin D (30 and 300 microM), NH4Cl (30 mM), and bafilomycin A1 (1 microM) significantly reduced the effect of HBP (10 microg/ml) to enhance LPS (10 ng/ml)-induced TNF-alpha release after 24 h. These findings demonstrate that internalization of HBP in monocytes is essential for the enhancement of LPS-induced TNF-alpha release. Transport of HBP to an activating compartment depends on intact F-actin polymerization and endosomal acidification, an important mechanism for endosomal protein sorting and trafficking.  (+info)

Transfer of a cathelicidin peptide antibiotic gene restores bacterial killing in a cystic fibrosis xenograft model. (8/2987)

Recent studies suggest that the gene defect in cystic fibrosis (CF) leads to a breach in innate immunity. We describe a novel genetic strategy for reversing the CF-specific defect of antimicrobial activity by transferring a gene encoding a secreted cathelicidin peptide antibiotic into the airway epithelium grown in a human bronchial xenograft model. The airway surface fluid (ASF) from CF xenografts failed to kill Pseudomonas aeruginosa or Staphylococcus aureus. Partial reconstitution of CF transmembrane conductance regulator expression after adenovirus-mediated gene transfer restored the antimicrobial activity of ASF from CF xenografts to normal levels. Exposure of CF xenografts to an adenovirus expressing the human cathelicidin LL-37/hCAP-18 increased levels of this peptide in the ASF three- to fourfold above the normal concentrations, which were equivalent in ASF from CF and normal xenografts before gene transfer. The increase of LL-37 was sufficient to restore bacterial killing to normal levels. The data presented describe an alternative genetic approach to the treatment of CF based on enhanced expression of an endogenous antimicrobial peptide and provide strong evidence that expression of antimicrobial peptides indeed protects against bacterial infection.  (+info)

*Beta defensin

"Tracheal antimicrobial peptide, a novel cysteine-rich peptide from mammalian tracheal mucosa: Peptide isolation and cloning of ... Defensins are 2-6 kDa, cationic, microbicidal peptides active against many Gram-negative and Gram-positive bacteria, fungi, and ... Ostriches have a genome containing the gene coding for the antimicrobial peptide, Ostricacin-1. The presence of this peptide ... When the receptors are activated a cascade reaction will take place and substances such as cytokines and antimicrobial peptides ...

*MP196

... is a synthetic antimicrobial peptide. It falls under the structural class: short cationic peptides. Since it is a short ... "Small cationic antimicrobial peptides delocalize peripheral membrane proteins". Proceedings of the National Academy of Sciences ... MP196 can be used as the key structure in order to develop any potential antimicrobial peptides which could help in fighting ... as this cationic peptide prefers incorporating into membranes which have a higher negatively charged phospholipid ratio. ...

*Indolicidin

... is an antimicrobial peptide. In vitro pharmacokinetics of antimicrobial cationic peptides alone and in combination ...

*Lactoferricin

... is an amphipathic, cationic peptide with anti-microbial and anti-cancer properties. It can be generated by the ... and sequences from within this fragment are also antimicrobial. The MilkAMP database contains a total of 111 peptides (natural ... a comprehensive database of antimicrobial peptides of dairy origin". Dairy Science & Technology. 94: 181-193. 2013. doi:10.1007 ...

*Lipid A acylase

PagP confers resistance to certain cationic antimicrobial peptides produced during the host innate immune response. Hwang, P. M ... Antimicrobial peptide resistance and lipid A acylation protein PagP is a family of several bacterial antimicrobial peptide ...

*Formyl peptide receptor 2

Cathelicidin-related antimicrobial peptides, numerous Pleurocidins which are a family of cationic antimicrobial peptides found ... which is a frog-derived antimicrobial peptide ((pro-inflammatory products derived from host anti-microbial proteins); and j) ... Eicosanoid receptor Formyl peptide receptor Lipoxin Resolvin Formyl peptide receptor 1 Formyl peptide receptor 3 GRCh38: ... Pundir P, Catalli A, Leggiadro C, Douglas SE, Kulka M (Jan 2014). "Pleurocidin, a novel antimicrobial peptide, induces human ...

*GDF2

... is a potent inducer of hepcidin (a cationic peptide that has antimicrobial properties) in liver cells (hepatocytes) and ... GDF2 contains an N-terminal TGF-beta-like pro-peptide (prodomain) (residues 56-257) and a C-terminal transforming growth factor ...

*Antimicrobial peptides

Calculated spatial positions of peptides in the lipid bilayer Antimicrobial Cationic Peptides at the US National Library of ... including antimicrobial peptides, in a protein sequence PeptideRanker Bioactive peptide, including antimicrobial peptide, ... Insect antimicrobial peptides OPM Amphibian antimicrobial peptides. OPM Niedermaier, Henry (9 February 2012). "SYNTHETIC MIMICS ... In the competition of bacterial cells and host cells with the antimicrobial peptides, antimicrobial peptides will ...

*Beta-defensin 2

Human beta-defensin-2 (hBD-2) is a cysteine-rich cationic low molecular weight antimicrobial peptide recently discovered in ... Beta-defensin 2 (BD-2) also known as skin-antimicrobial peptide 1 (SAP1) is a peptide that in humans is encoded by the DEFB4 ( ... 1999). "Production of beta-defensin antimicrobial peptides by the oral mucosa and salivary glands". Infect. Immun. 67 (6): 2740 ... 2001). "Production of beta-defensin antimicrobial peptides by maxillary sinus mucosa". American journal of rhinology. 15 (3): ...

*Histone H2A

Antimicrobial peptide: Histones are conserved eukaryotic cationic proteins present in the cells and are involved in the ... Histone H2A variant is reported to be involved in host immune response by acting as antimicrobial peptides (AMPs). H2A are α- ... 2013). "An unconventional antimicrobial protein histone from freshwater prawn Macrobrachium rosenbergii: Analysis of immune ... amphipathic protein with hydrophobic and hydrophilic residues on opposing sides that enhances the antimicrobial activity of H2A ...

*Fixation (histology)

"Antibacterial Action of Structurally Diverse Cationic Peptides on Gram-Positive Bacteria". Antimicrobial Agents and ...

*Liver-expressed antimicrobial peptide

LEAP2 is a cysteine-rich, and cationic protein. LEAP2 contains a core structure with two disulphide bonds formed by cysteine ... Liver-expressed antimicrobial peptides are a family of mammalian liver-expressed antimicrobial peptides (LEAP). The exact ... In contrast to smaller LEAP-2 variants, this peptide exhibits dose-dependent antimicrobial activity against selected microbial ... a novel blood peptide expressed in the liver". Protein Sci. 12 (1): 143-52. doi:10.1110/ps.0213603. PMC 2312392 . PMID 12493837 ...

*Azurocidin 1

Azurocidin also known as cationic antimicrobial protein CAP37 or heparin-binding protein (HBP) is a protein that in humans is ... Pereira HA, Erdem I, Pohl J, Spitznagel JK (1993). "Synthetic bactericidal peptide based on CAP37: a 37-kDa human neutrophil ... "Entrez Gene: AZU1 azurocidin 1 (cationic antimicrobial protein 37)". Linder A, Christensson B, Herwald H, Björck L, Akesson P ( ... granule-associated cationic antimicrobial protein chemotactic for monocytes". Proc. Natl. Acad. Sci. U.S.A. 90 (10): 4733-7. ...

*Oxyopinin

It is also 29% identical to the amino acid residues of the frog antimicrobial peptide dermaseptin. Oxyopinins 2a, 2b, 2c, and ... They are the largest linear cationic amphipathic peptides detected in the venom of any spider. They are structurally α-helical ... The sixth peptide oxyopinin 4a (Oxt-4a) was reported in 2011 from Oxyopes takobius. It is composed of 77 amino acid residues, ... The first five peptides, namely oxyopinins 1, 2a, 2b, 2c, and 2d, were described in 2002 from Oxyopes kitabensis. Oxyopinin 1 ...

*Cell-penetrating peptide

Helical β-peptides mimic antimicrobial activities of host defense peptides. This feature requires the orientation of cationic - ... In the absence of linker, the cationic peptide can interact more efficient with the target cell and cellular uptake occurs ... Cell-penetrating peptides (CPPs) are short peptides that facilitate cellular intake/uptake of various molecular equipment (from ... β-Peptides are conformationally more stable in aqueous solution than naturally occurring peptides, especially for small chains ...

*Phagocytosis

... and cationic proteins such as defensins. Other antimicrobial peptides are present in these granules, including lactoferrin, ...

*OmpT

... the host releases antimicrobial peptides as part of the innate immune response. Since OmpT can break down these antimicrobials ... but OmpT easily degrades the cationic protamine peptides, thus enhancing the risk of infection. There is a genetic link between ... Once docked in this position, water is positioned to attack the peptide in the active site. The cleavage of peptide bonds by ... "Identification of OmpT as the protease that hydrolyzes the antimicrobial peptide protamine before it enters growing cells of ...

*Magainin

The magainins are a class of antimicrobial peptides found in the African clawed frog (Xenopus laevis). The peptides are ... cationic, generally lack a stable conformation in water but form amphipathic α-helix in membranes; their mechanism against ... "Host-defense peptides in skin secretions of African clawed frogs (Xenopodinae, Pipidae)". General and comparative endocrinology ...

*Dermaseptin

Mor, A. (2000) Peptide-based antibiotics: a potential answer to raging antimicrobial resistance. Drug Dev Res, 50:440-447. ... Hancock, R.E.W., Falla, T. and Brown, M.H. (1995) Cationic bactericidal peptides. Adv Microb Physiol, 37:135-75. Amiche, M., ... antimicrobial resistance Amiche, M., Seon, A.A., Pierre, T.N. and Nicolas, P. (1999) The dermaseptin precursors: a protein ... After transient loading of the cells with the non-toxic dermaseptin S4 analogue K4-S4(1-13)a, the peptide is transported in the ...

*Defensin

... s are antimicrobial peptides that act mainly by disrupting the structure of bacterial cell membranes and are found in ... The name 'defensin' was coined in the mid1980s, though the proteins had been studied as 'Cationic Antimicrobial Proteins'. The ... a plant peptide signal involved in the innate immune response". Peptides. 29 (12): 2083-9. doi:10.1016/j.peptides.2008.08.019. ... In this sense, the intestinal production of antimicrobial peptides as hBD2 and hBD4 by trefoil from milk might play an ...

*Class II bacteriocin

It includes the alpha enterocins and lactococcin G peptides. These peptides have some antimicrobial properties; they inhibit ... This cationic N-terminal beta-sheet domain mediates binding of the class IIa bacteriocin to the target cell membrane. The C- ... Fimland G, Nissen-Meyer J, Johnsen L (2005). "The C-terminal domain of pediocin-like antimicrobial peptides (class IIa ... Dalhus B, Fimland G, Nissen-Meyer J, Johnsen L (2005). "Pediocin-like antimicrobial peptides (class IIa bacteriocins) and their ...

*Cathelicidin

The cathelicidin family of peptides are classified as antimicrobial peptides (AMPs). The AMP family also includes the defensins ... "Cell differentiation is a key determinant of cathelicidin LL-37/human cationic antimicrobial protein 18 expression by human ... CAMP cathelicidin antimicrobial peptide". Zanetti M (January 2004). "Cathelicidins, multifunctional peptides of the innate ... Cathelicidin-related antimicrobial peptides are a family of polypeptides found in lysosomes of macrophages and ...

*Cliotide

Butelase 1 is the fastest peptide ligase known capable of catalyzing peptide cyclization at an extraordinary efficiency. Nguyen ... At a concentration of 1 μM, cationic cliotides are capable of augmenting the secretion of various cytokines and chemokines in ... Cliotides display in vitro antimicrobial activity against E. coli, K. pneumoniae, and P. aeruginosa and cytotoxicity against ... Cliotides are a group of related peptides that have been isolated from the heat-stable fraction of Clitoria ternatea (butterfly ...

*Mesobuthus eupeus

A number of antimicrobial peptides have also been found in the venom of M. eupeus. Meucin-13 and Meucin-18 exhibited extensive ... "Characterization of two linear cationic antimalarial peptides in the scorpion Mesobuthus eupeus". Biochimie. 92 (4): 350-9. doi ... of two genetically related meucin peptides highlights evolutionary divergence and convergence in antimicrobial peptides". The ...

*Defensin, alpha 1

Human alpha defensin 1 belongs to the alpha defensin family of antimicrobial peptides. Defensins are a family of microbicidal ... Cationic 29-30 amino acids HNPs associate with the negatively charged proteoglycan serglycin and translocate to azurophil ... Zhang XL, Selsted ME, Pardi A (1992). "NMR studies of defensin antimicrobial peptides. 1. Resonance assignment and secondary ... 1992). "NMR studies of defensin antimicrobial peptides. 2. Three-dimensional structures of rabbit NP-2 and human HNP-1". ...

*Arenicin

"Solution Structures and Biological Functions of the Antimicrobial Peptide, Arenicin-1, and Its Linear Derivative". Peptide ... Cationic residues on Arenicin-3 interacts with negatively charged lipopolysaccharides on the outer membrane of Gram-negative ... Arenicin is the name used to classify a group of antimicrobial peptides, which have shown promise as a novel drug to combat ... "NZ17074 - A Novel antimicrobial peptide showing potent in vitro activity against gram negative multi-resistant clinical ...
TY - JOUR. T1 - Improvement of outer membrane-permeabilizing and lipopolysaccharide- binding activities of an antimicrobial cationic peptide by C-terminal modification. AU - Piers, K. L.. AU - Brown, M. H.. AU - Hancock, Robert. PY - 1994/1/1. Y1 - 1994/1/1. N2 - Antimicrobial cationic peptides have been discovered in many different organisms and often possess a broad range of activity. In this study, we investigated the mechanisms of actions of melittin and two synthetic peptides, CEME (a cecropin-melittin hybrid) and CEMA, against gram-negative bacteria. CEMA was produced by recombinant DNA procedures and is an analog of CEME with a modified C terminus resulting in two additional positive charges. All three peptides showed good antimicrobial activity against four different gram-negative bacteria, but only CEMA was able to somewhat augment the activity of some conventional antibiotics in synergy studies. Studies using the bacteria Pseudomonas aeruginosa and Enterobacter cloacae showed that the ...
114118PRTArtificial SequenceSynthetic antimicrobial polypeptide 1Lys Asn Leu Arg Arg Ile Ile Arg Lys Gly Ile His Ile Ile Lys Lys1 5 10 15Tyr Gly218PRTArtificial SequenceSynthetic antimicrobial polypeptide 2Lys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa1 5 10 15Xaa Xaa318PRTArtificial SequenceSynthetic antimicrobial polypeptide 3Lys Arg Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa1 5 10 15Xaa Xaa418PRTArtificial SequenceSynthetic antimicrobial polypeptide 4Lys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Arg Xaa Xaa Xaa Xaa1 5 10 15Xaa Xaa518PRTArtificial SequenceSynthetic antimicrobial polypeptide 5Lys Gly Leu Arg Arg Ile Ile Arg Lys Gly Ile His Ile Ile Lys Lys1 5 10 15Tyr Gly618PRTArtificial SequenceSynthetic antimicrobial polypeptide 6Lys Gly Leu Arg Arg Ile Ile Arg Trp Gly Ile His Ile Ile Lys Lys1 5 10 15Tyr Gly718PRTArtificial SequenceSynthetic antimicrobial polypeptide 7Lys Ile Leu Arg Arg Ile Ile Arg Lys Gly Ile His Ile Ile Lys Lys1 5 10 15Tyr Gly818PRTArtificial ...
Peters interests lie in studying the human innate immune system with a view to developing novel therapeutics for infections. He is particularly interested in studying the activities of Cationic Host Defence Peptides (CHDP), principally in the context of viral respiratory infections, such as influenza. Cationic Host Defence Peptides, also known as antimicrobial peptides, are key components of the immune response. These peptides have been shown to display a broad spectrum of antimicrobial and immunomodulatory activities and, as such, are exciting targets for novel therapeutics. The concepts of Peters work can be expanded to broadly include many different types of inflammatory and infectious conditions, but are unified by the common interest in the multiple roles of cationic host defence peptides in the immune response ...
PURPOSE Antimicrobial peptides (AMPs) are cationic host defense peptides with microbicidal and cell-signaling properties. They show promise as potential therapeutic agents. In the present study, a beta-defensin AMP gene was isolated from the ocular surface for the first time, and its expression was characterized in the presence of ocular inflammation and/or infection. METHODS Total RNA was obtained from impression cytology samples of the conjunctiva and cornea of normal patients and of those with bacterial, viral, acanthamoeba, or dry eye disease. The expression of the beta-defensin AMP DEFB-109 was determined by using reverse transcription-polymerase chain reaction (RT-PCR). Relative quantification of the gene in the various groups was performed by means of real-time PCR. RESULTS DEFB-109 was constitutively expressed in all samples. The gene showed significantly decreased expression in the presence of all types of inflammation/infection. Reduced expression featured most prominently in acanthamoeba
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Disclosed are novel bactericidal/permeability-increasing (BPI) protein products wherein cysteine residue number 132 or 135 is replaced by another amino acid residue, preferably an alanine or serine residue and/or wherein the leucine residue at position 193 is the carboxy terminal residue. Also disclosed are DNA sequences encoding methods for the production of the same in appropriate host cells, and stable homogeneous pharmaceutical compositions containing the analogs suitable for use treatment of gram negative bacterial infection and its sequelae.
Barlow, P. G., Li, Y., Wilkinson, T. S., Bowdish, D. M. E., Lau, Y. E., Cosseau, C., …Davidson, D. J. (2005). The human cationic host defense peptide LL-37 mediates contrasting effects on apoptotic pathways in different primary cells of the innate immune system. Journal of Leukocyte Biology. 80, 509-520. doi:10.1189/jlb.1005560. ISSN 0741-5400. ...
Barlow, P. G., Li, Y., Wilkinson, T. S., Bowdish, D. M. E., Lau, Y. E., Cosseau, C., …Davidson, D. J. (2005). The human cationic host defense peptide LL-37 mediates contrasting effects on apoptotic pathways in different primary cells of the innate immune system. Journal of Leukocyte Biology. 80, 509-520. doi:10.1189/jlb.1005560. ISSN 0741-5400. ...
Background: Antimicrobial peptides (AMPs) are synthesized and secreted by immune and epithelial cells that are constantly exposed to environmental microbes. AMPs are essential for barrier defense and deficiencies lead to increased susceptibility to infection. In addition to their ability to disrupt the integrity of bacterial, viral and fungal membranes, AMPs bind lipopolysaccharides, act as chemoattractants for immune cells and bind to cellular receptors and modulate the expression of cytokines and chemokines. These additional biological activities may explain the role of AMPs in inflammatory diseases and cancer. Modulating the endogenous expression of AMPs offers potential therapeutic treatments for infection and disease. Methods: The present review examines published data from both in vitro and in vivo studies reporting effects of nutrients and byproducts of microbial metabolism on the expression of antimicrobial peptide genes in order to highlight an emerging appreciation for the role of ...
Host defense peptides (HDPs) are an important first line of defense with antimicrobial and immunomoduatory properties. Because they act on the microbial membranes or host immune cells, HDPs pose a low risk of triggering microbial resistance and therefore, are being actively investigated as a novel class of antimicrobials and vaccine adjuvants. Cathelicidins and β-defensins are two major families of HDPs in avian species. More than a dozen HDPs exist in birds, with the genes in each HDP family clustered in a single chromosomal segment, apparently as a result of gene duplication and diversification. In contrast to their mammalian counterparts that adopt various spatial conformations, mature avian cathelicidins are mostly α-helical. Avian β-defensins, on the other hand, adopt triple-stranded β-sheet structures similar to their mammalian relatives. Besides classical β-defensins, a group of avian-specific β-defensin-related peptides, namely ovodefensins, exist with a different six-cysteine motif. Like
1. SteinerH, HultmarkD, EngstromA, BennichH, BomanHG (1981) Sequence and specificity of two antibacterial proteins involved in insect immunity. Nature 292: 246-248.. 2. ZasloffM (1987) Magainins, a class of antimicrobial peptides from Xenopus skin: isolation, characterization of two active forms, and partial cDNA sequence of a precursor. Proc Natl Acad Sci USA 84: 5449-5453.. 3. GanzT, SelstedME, LehrerRI (1990) Defensins. Eur J Haematol 44: 1-8.. 4. BrogdenKA (2005) Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria? Nature Rev Microbiol 3: 238-250.. 5. HancockREW, SahlH-G (2006) Antimicrobial and host-defense peptides as new anti-infective therapeutic strategies. Nature Biotech 24: 1551-1557.. 6. PeschelA, SahlH-G (2006) The co-evolution of host cationic antimicrobial peptides and microbial resistance. Nature Rev Microbiol 4: 529-536.. 7. HaleJDF, HancockREW (2007) Alternative mechanisms of action of cationic antimicrobial peptides on bacteria. Expert Rev Anti Infect Ther ...
National Pirogov Memorial Medical University, Vinnytsya, Ukraine Purpose - to determine the activity of antimicrobial peptides, such as cathelicidin LL-37 and 25-hydroxycholecalciferol in children with asthma. Materials and methods. We have comprehensively examined 200 children with asthma aged 6 to 17 years. The contents of 25(OH)D3 and cathelicidin LL-37 in serum were determined by ELISA according to the instructions of the manufacturer. Results. The examination revealed that the total content of 25(OH)D3 in the serum of children with asthma differs from the values of healthy children and characterized by a significant decrease of its level (p,0.01). Concentration of cathelicidin LL-37 in patients with asthma was significantly higher (p,0.001), than in the group of healthy children. The positive correlation between the cathelicidin LL-37, interleukin 1 (rxy=0.398 (p=0.02)) and interleukin 6 (rxy=0.178 (p=0.034)) in children with asthma was determined. The concentration of cathelicidin LL-37 in ...
There has been increasing concern regarding the emergence of multi-drug resistant pathogens. The resistance develops when pathogens, especially bacteria, are frequently exposed to conventional antibiotics, as they are heavily used in both human and livestock. This is due to the high target specificity of conventional antibiotics, which places pathogens in high selective pressures and eventually results in drug resistant by mutations. To address this issue, global actions and cooperation are needed. At the same time, new technologies and strategies need to be developed. Host defense peptides (HDPs) are widely found in the innate immune system. They show both direct antimicrobial properties and immunomodulatory activities. The multifaceted functions of HPDs make them less likely to promote antimicrobial resistance. Thus, they are promising as new therapeutics to treat multi-drug resistant infections. In fact, several drug candidates derived from HDPs have entered the clinical trial, but none of them got
Host defense peptides (HDPs) are positively-charged and amphipathic components of the innate immune system that have demonstrated great potential to become the next generation of broad spectrum therapeutic agents effective against a vast array of pathogens and tumor. As such, many approaches have been taken to improve the therapeutic efficacy of HDPs. Amongst these methods, the incorporation of d-amino acids (d-AA) is an approach that has demonstrated consistent success in improving HDPs. Although, virtually all HDP review articles briefly mentioned about the role of d-AA, however it is rather surprising that no systematic review specifically dedicated to this topic exists. Given the impact that d-AA incorporation has on HDPs, this review aims to fill that void with a systematic discussion of the impact of d-AA on HDPs.. ...
Donald J Davidsons lab studies the role of cationic host defence peptides (antimicrobial peptides) as modulators of cell death, inflammation and immunity in infectious and inflammatory lung diseases, and innate immune signalling.
Host defense peptides (HDPs) are short antimicrobial peptides of the innate immune system. Deficiencies in HDPs contribute to enhanced susceptibility to infections, e.g., in cystic fibrosis (CF). Exogenous HDPs can compensate for these deficiencies, but their development as antimicrobials is limited by cytotoxicity. Three HDP prodrugs were designed so their net positive charge is masked by a promoiety containing a substrate for the enzyme neutrophil elastase (NE). This approach can confine activation to sites with high NE levels. Enzyme-labile peptides were synthesized, and their activation was investigated using purified NE. Susceptibilities of Pseudomonas aeruginosa to parent and prodrug peptides in the presence and absence of NE-rich CF human bronchoalveolar lavage (BAL) fluid and different NaCl concentrations were compared. The effect of the HDP promoiety on cytotoxicity was determined with cystic fibrosis bronchial epithelial (CFBE41o-) cells. NE in CF BAL fluids activated the HDP prodrugs,
This comprehensive database for antimicrobial peptides is manually curated based on a set of data-collection criteria. There are 139 human host defense peptides, 305 from mammals annotated, 1087 active peptides from amphibians (1018 from frogs), 134 fish peptides, 45 reptile peptides, 42 from birds, 559 from arthropods, [310 from insects, 69 from crustaceans, 7 from myriapods, 171 from chelicerata, (43 from spiders, 88 from scorpions)], 45 from molluscs, 6 AMPs from protozoa, and more. Of the 428 unique NMR/X-ray diffracted 3D structures annotated for host defense peptides in the APD, 301 with coordinates deposited in the Protein Data Bank (PDB) can be directly rotated, zoomed, and viewed. Top left: Amphibian α-helical magainin II; Top right: bovine β-sheet lactoferricin; Bottom left: plant αβ-PsD1; Bottom right: bovine non-αβ indolicidin. This original database consists of a pipeline of search functions for innate immune peptides. You can search for peptide information using APD ID, ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Host defence peptides (HDPs) are polypeptide sequences found ubiquitously in nature that have garnered significant attention as alternatives to antibiotics. Originally appreciated for their direct antibacterial effect, recent work has revealed that many HDPs possess antibiofilm activity, anticancer activity and/or the ability to modulate the immune response of the host.
Many lepidopteran insects are agricultural pests that affect stored grains, food and fiber crops. These insects have negative ecological and economic impacts since they lower crop yield, and pesticides are expensive and can have off-target effects on beneficial arthropods. A better understanding of lepidopteran immunity will aid in identifying new targets for the development of specific insect pest management compounds. A fundamental aspect of immunity, and therefore a logical target for control, is the induction of antimicrobial peptide (AMP) expression. These peptides insert into and disrupt microbial membranes, thereby promoting pathogen clearance and insect survival. Pathways leading to AMP expression have been extensively studied in the dipteran Drosophila melanogaster. However, Diptera are an important group of pollinators and pest management strategies that target their immune systems is not recommended. Recent advances have facilitated investigation of lepidopteran immunity, revealing both
Bactericidal/permeability-increasing protein (BPI), a cationic protein isolated from human neutrophils, binds lipopolysaccharide (LPS), kills gram-negative bacteria, and neutralizes many of the effects of LPS in vitro and in vivo. We hypothesized that a recombinant 23-kDa NH2-terminal fragment of BPI (BPI23) would reduce acute lung injury in endotoxemic pigs. At -18 h, pigs received an intravenous priming dose of LPS (20 micrograms/kg). Anesthetized ventilated swine were randomized to receive 1) no further treatment (n = 4); 2) LPS (250 micrograms/kg over 50 min) and BPI23 (3-mg/kg bolus and 3 mg/kg over 60 min) (n = 6); or 3) LPS and thaumatin, a cationic protein devoid of LPS neutralizing activity that has a molecular mass and isoelectric point that are similar to that of BPI23 (n = 7). BPI23 treatment significantly ameliorated LPS-induced hypoxemia, functional upregulation of opsonin receptors on circulating phagocytes, and alveolitis but had no effect on the elaboration of tumor necrosis ...
CUONG, Ng.V. et al. Polymorphisms of candidate genes associated with meat quality and disease resistance in indigenous and exotic pig breeds of Vietnam. S. Afr. j. anim. sci. [online]. 2012, vol.42, n.3, pp.221-231. ISSN 2221-4062.. The objectives of this study were to analyse genotype distribution and sequence variations of candidate genes putatively associated with meat quality and disease resistance in exotic and indigenous Vietnamese pig breeds. For this purpose, 340 pigs from four indigenous and two exotic breeds were included in the analysis of the polymorphisms of the heart fatty-acid-binding protein (H-FABP), alpha 1 fucosyltransferase (FUT1), and bactericidal/permeability-increasing protein (BPI) genes by the sequencing and PCR-RFLP methods. For H-FABP, 17 single nucleotide polymorphisms (SNPs) were detected in indigenous pig breeds by direct sequencing of a fragment at intron 2 of the H-FABP gene. The mutation T1556C created a new restriction site for the enzyme MspI, which gave rise ...
We have studied the mechanism of action of representatives of most of these classes. Basically these peptides interact with the surface of Gram negative bacteria and are taken up by self-promoted uptake. They then insert into the cytoplasmic membrane under the influence of the transmembrane electrical potential gradient (which in bacteria is about -150 mV oriented internal negative so as to electrophorese the cationic peptides towards the membrane). They assemble in the membrane into multi-state channels which we have described via the "aggregate model", and in many cases cross the cytoplasmic membrane to access cytoplasmic targets, or in some cases permeabilize the cytoplasmic membrane barrier. It should be stated that in the past many people in the antimicrobial peptide field favoured the latter mechanism for most peptides; however, our own published evidence appears to be more consistent with cytoplasmic targets for many peptides.. The better cationic peptides act very rapidly (within ...
This application is in response to PA-09-164 (NIH Exploratory Developmental Research Grant Program). Given the high rate of hospital-acquired infection in criti...
Sandrine Ménard, Valentina Förster, Michael Lotz, Dominique Gütle, Claudia U. Duerr, Richard L. Gallo, Birgitta Henriques-Normark, Katrin Pütsep, Mats Andersson, Erik O. Glocker, Mathias W. Hornef ...
The increasing occurrence of antibiotic-resistant bacteria is spurring the search for new classes of antibiotics. Naturally occurring antimicrobial peptides have gained a lot of attention for many reasons: they are naturally occurring, there is huge variety of antimicrobial peptides that can be evaluated for potential drug leads, and antimicrobial peptides are rapidly broken down by protease enzymes which reduces the possibility of bacteria developing resistance.. Before antimicrobial peptide drugs come into wide use, we need to mindful that although resistance to antimicrobial peptides is not common at this time, bacteria can develop resistance. Certain biotypes of the human intestinal pathogen Vibro choerae can gain resistance to cationic antimicrobial peptides through modification of lipid A in their cell membranes (CM Herrera, et al., Mol. Microbiol., 2017, Sept. 14 Epub. ahead of print). To prevent the development of resistance, new peptide antibiotics must be used intelligently.. We should ...
TY - CONF. T1 - Versatile interactions of the antimicrobial peptide novispirin with detergents and lipids. AU - Wimmer, Reinhard. AU - Andersen, Kell. AU - Davidsen, Mads. AU - Mølgaard, Søren. AU - Nesgaard, Lise. AU - Kristensen, Hans Henrik. AU - Vad, Brian. AU - Otzen, Daniel. PY - 2006. Y1 - 2006. M3 - Paper without publisher/journal. Y2 - 20 August 2006 through 26 August 2006. ER - ...
DUGi: Viewing Item from repository Recercat: The presence of the antimicrobial peptide (AMP) biosynthetic genes srfAA (surfactin), bacA (bacylisin), fenD (fengycin), bmyB (bacyllomicin), spaS (subtilin), and ituC (iturin) was examined in 184 isolates of Bacillus spp. obtained from plant environments (aerial, rhizosphere, soil) in the Mediterranean land area of Spain. Most strains had between two and four AMP genes whereas strains with five genes were seldom detected and none of the strains had six genes. The most frequent AMP gene markers were srfAA, bacA, bmyB, and fenD, and the most frequent genotypes srfAA-bacA-bmyB and srfAAbacA-bmyB-fenD. The dominance of these particular genes in Bacillus strains associated with plants reinforces the competitive role of surfactin, bacyllomicin, fengycin, and bacilysin in the fitness of strains in natural environments. The use of these AMP gene markers may assist in the selection of putative biological control agents of plant pathogens
A method pioneered by MIT researchers might offer hope in finding a new generation of antibiotics, made of antimicrobial peptides. Antimicrobial peptides a
Antimicrobial peptides (AMPs) are host-defense molecules produced by all living organisms, including bacteria, fungi, plants, invertebrates, and vertebrates. The peptides exhibit potent cytotoxic activity against all microbes, including bacteria, fungi, viruses, and parasites. There is now evidence in humans and other mammals that these peptides represent a key component of host defense at epithelial surfaces, the barrier between the external environment and the interior milieu, interacting with both symbionts and pathogens. Members of these large families also have activities far beyond being directly antimicrobial, including immunity, wound healing, fertility, and as ligands for numerous receptors leading to the induction of a variety of signaling pathways. Furthermore, significant research has been carried out to develop these naturally occurring peptides as therapeutic agents against microbial infections. The 2013 Gordon Conference on Antimicrobial Peptides will present the latest research ...
Azurocidin, also known as cationic antimicrobial protein 37 kDa (CAP37) or heparin-binding protein (HBP) is an inactive homolog of serine proteinases residing in granulocytes. The ability to cleave peptide bond was lost due to replacement of two of the three residues from the conserved catalytic triad characteristic for serine proteinases. Azurocidin has a broad spectrum of antimicrobial activity, mainly against Gram-negative bacteria. It is also recognized as a multifunctional inflammatory mediator for its contracting effects on endothelial cells causing an increase of vascular permeability, capacity to bind endotoxin and ability to attract monocytes to inflammation sites ...
Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function ...
சளிப்படலம் போன்ற ஒரு வண்ணமயமான திரவத்தினை சுரக்கும் தவளையானது (ஹைட்ரோஃபிளாஸ் பேஹுவிஸ்தாரா)( Hudrophylax bahuvistara) கேளராவில் கண்டுபிடிக்கப்பட்டு உள்ளது. தவளையின் தோலிலிருந்து வெளிப்படும் பிசுப்பிசுப்பான திரவத்தினை ஆய்வு செய்ததில் host defence peptides இருப்பது கண்டுபிடிக்கப்பட்டு உள்ளது. இந்த புதிய பெப்டைட்களுக்கு உருமின் என்று கேரளாவின் உறுமி வாளை நினைவுபடுத்தும் வகையில் பெயர்
BHP corresponds to residues 33-61 of bovine hemoglobin and was generated in the tick gut for defense. Note that whole hemoglobin of the cow is not antimicrobial but is used as a source for antimicrobial peptide production. Active against Gram+ M. luteus A270 (MIC 5 uM), S. epidermidis (MIC 21 uM), and fungi C. albicans (MIC 5 uM), S. cerevisiae (MIC 11), and A. nidulans (MIC 1.3 uM). Updated 2/2019 ...
A three-year PhD position is available at the GEC Department, University "Picardie Jules Verne" (UPJV) in Amiens, France, to study the interaction of antimicrobial peptides with biomimetic membranes.. Scientific background. Antimicrobial peptides (AMPs) form a very large family of natural peptides with an astonishing variety of activities (antibacterial, anti-fungal, antiviral and anti-tumor) with an impact in medicine (as drugs targeting cancer or antibiotic-resistant super-bugs) and agriculture (as an alternative to pesticides). They are produced in all living kingdoms, from bacteria to humans, including plants, insects and animals, playing a key role in immunity. With the threat of multi-drug resistant bacteria, already appearing in mainstream information, there is no wonder that great interest has been directed towards these simple molecules. In agriculture, the use of optimized AMPs might address the emergency of the environmental damage caused by the use of pesticides. With the advent of ...
The effectiveness of antimicrobial compounds can be easily screened, however their mechanism of action is much more difficult to determine. Many compounds act by compromising the mechanical integrity of the bacterial cell envelope, and our study introduces an AFM-based creep deformation technique to evaluate Interaction of nano-objects with lipid membranes
1F0E: Role of the hinge region and the tryptophan residue in the synthetic antimicrobial peptides, cecropin A(1-8)-magainin 2(1-12) and its analogues, on their antibiotic activities and structures.
abstract = {The antimicrobial defence of Drosophila relies largely on the challenge-induced synthesis of an array of potent antimicrobial peptides by the fat body. The defence against Gram-positive bacteria and natural fungal infections is mediated by the Toll signalling pathway, whereas defence against Gram-negative bacteria is dependent on the Immune deficiency (IMD) pathway. Loss-of-function mutations in either pathway reduce the resistance to corresponding infections. The link between microbial infections and activation of these two pathways has remained elusive. The Toll pathway is activated by Gram-positive bacteria through a circulating Peptidoglycan recognition protein (PGRP-SA). PGRPs appear to be highly conserved from insects to mammals, and the Drosophila genome contains 13 members. Here we report a mutation in a gene coding for a putative transmembrane protein, PGRP-LC, which reduces survival to Gram-negative sepsis but has no effect on the response to Gram-positive bacteria or ...
Over the last decade, antimicrobial peptides (AMPs) have emerged as a promising alternative for treatment of various infections. A large variety of AMPs have been identified and isolated from plants, animals and humans. The benefits of using AMPs are that they act by disrupting the bacteria membranes, a mechanism that is fast and non-specific. Therefore bacteria are not prone to develop high level resistance towards these compounds in the same extent as towards conventional antibiotics. AMPs are in general rather small (10-40 amino acid residues), cationic and amphiphilic compounds with a diversity in secondary structure which can be altered to different extent upon membrane interaction. Their structure has been developed for millenia during evolution and is today well preserved.. AMPs have been assessed, analyzed and modified in order to increase their function and efficiency for future drug delivery applications. In humans, AMPs are naturally present in high concentrations in tissues ...
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Naturally present in the skin, a precursor in the biosynthesis of cholesterol and vitamin D3. Regulates skin cell differentiation and stimulates skin natural antimicrobial peptides. A 1% solution is also available ...
Multidrug antibiotic resistance is an increasingly serious public health problem worldwide. Thus, there is a significant and urgent need for the development of new classes of antibiotics that do not induce resistance. To develop such antimicrobial compounds, we must look toward agents with novel mechanisms of action. Membrane-permeabilizing antimicrobial peptides (AMPs) are good candidates because they act without high specificity toward a protein target, which reduces the likelihood of induced resistance. Understanding the mechanism of membrane permeabilization is crucial for the development of AMPs into useful antimicrobial agents. Various models, some phenomenological and others more quantitative or semimolecular, have been proposed to explain the action of AMPs. While these models explain many aspects of AMP action, none of the models captures all of the experimental observations, and significant questions remain unanswered. Here, we discuss the state of the field and pose some questions ...
Creative Peptides offers Omiganan for your research. We also provide custom peptide synthesis, process development, GMP manufacturing.
1F0F: Role of the hinge region and the tryptophan residue in the synthetic antimicrobial peptides, cecropin A(1-8)-magainin 2(1-12) and its analogues, on their antibiotic activities and structures.
Antimicrobial peptides (AMPs) are naturally occurring entities with potential as pharmaceutical candidates and/or food additives. They are present in many organisms including bacteria, insects, fish and mammals. While their antimicrobial activity is equipotent with many commercial antibiotics, current limitations are poor pharmacokinetics, stability and potential toxicology issues. Most elicit antimicrobial action via perturbation of bacterial membranes. Consequently, associated cytotoxicity in human cells is reflected by their capacity to lyse erythrocytes. However, more rigorous toxicological assessment of AMPs is required in order to predict potential failure at a later stage of development.Wedescribe a high-content analysis (HCA) screening protocol recently established for determination and prediction of safety in pharmaceutical drug discovery. HCA is a powerful, multi-parameter bioanalytical tool that amalgamates the actions of fluorescence microscopy with automated cell analysis software ...
Cationic antimicrobial peptides are becoming crucial for animal defense. The peptides can be induced through bacteria or other products. The peptide has a wide range of bacterial strains and this include antibiotic resistance. They have the potential to kill quickly and delay the selection of resistant mutants. These are synergistic to conventional antibiotics, lysozyme and other peptides. These will be able to kill the bacteria especially in test animals. The name given to it is bacterial infection and can be treated with antibiotics leading to the release of lipoteichoic acid and lipopolysaccharide (LPS) (bacterial products) leading to lethal sepsis. The peptides prevent cytokine induction with the help of bacterial products found tissue culture. It is said to block the beginning of sepsis in mouse models.. An array of experiments is conducted using macrophage cell and it has demonstrated a model peptide, it also blocks the reflection of the genes with induced LPS. The peptides initiates this ...
Background: The amyloid \(\beta\)-protein (A\(\beta\)) is believed to be the key mediator of Alzheimers disease (AD) pathology. A\(\beta\) is most often characterized as an incidental catabolic byproduct that lacks a normal physiological role. However, A\(\beta\) has been shown to be a specific ligand for a number of different receptors and other molecules, transported by complex trafficking pathways, modulated in response to a variety of environmental stressors, and able to induce pro-inflammatory activities. Methodology/Principal Findings: Here, we provide data supporting an in vivo function for A\(\beta\) as an antimicrobial peptide (AMP). Experiments used established in vitro assays to compare antimicrobial activities of A\(\beta\) and LL-37, an archetypical human AMP. Findings reveal that A\(\beta\) exerts antimicrobial activity against eight common and clinically relevant microorganisms with a potency equivalent to, and in some cases greater than, LL-37. Furthermore, we show that AD whole ...
In recent years, the number of people suffering from cancer and multi-resistant infections has increased, such that both diseases are already seen as current and future major causes of death. Moreover, chronic infections are one of the main causes of cancer, due to the instability in the immune system that allows cancer cells to proliferate. Likewise, the physical debility associated with cancer or with anticancer therapy itself often paves the way for opportunistic infections. It is urgent to develop new therapeutic methods, with higher efficiency and lower side effects. Antimicrobial peptides (AMPs) are found in the innate immune system of a wide range of organisms. Identified as the most promising alternative to conventional molecules used nowadays against infections, some of them have been shown to have dual activity, both as antimicrobial and anticancer peptides (ACPs). Highly cationic and amphipathic, they have demonstrated efficacy against both conditions, with the number of nature-driven or
RNA interference (RNAi) is a highly efficient and specific posttranscriptional gene silencing process, which can be triggered by small interfering RNA. The efficiency and specificity of this process makes siRNA a powerful tool for gene therapy. However, the use of siRNA is hampered by its rapid degradation and poor cellular uptake. A variety of carriers for example, virus, lipid, polymer, peptides, and nanoparticles, have been developed to protect siRNA and maximize its therapeutic effects. Among these carriers, peptides have emerged as a promising candidate due to their intrinsically rapid and highly efficient internalization and their typically low toxicity. The amphipathic, cationic peptide C6 (sequence: Ac-RLLRLLLRLWRRLLRLLR-NH2) was previously designed and studied in our group. C6 achieved significant siRNA cellular uptake but induced low gene silencing, possibly due to siRNA entrapment in endosomes. In order to enhance endosomal release of siRNA, two strategies were incorporated to design ...
Creative Peptides offers Pleurain-C1 antimicrobial peptide for your research. We also provide custom peptide synthesis, process development, GMP manufacturing.
D. Erturk-Hasdemir, M. Broemer, F. Leulier, W. S Lane, N. Paquette, D. Hwang, C. H Kim, S. Stoven, P. Meier, and N. Silverman (2009) Proc Natl Acad Sci U S A, 106(24):9779-84.. ...
The present invention provides antimicrobial peptides. The peptides are non-glycosylated, less than about 100 amino acids in length, and include an amino acid sequence selected from: AVESTVATLEAΣPEVIE
Abstract A novel approach for boosting antimicrobial peptides through end tagging with hydrophobic oligopeptide stretches is demonstrated. Focusing on two peptides derived from kin..
引用/出所元. admin. (2013年04月05日). How Microbes Evolve to Dodge the Membrane Disruptive Actions of Antimicrobial Peptides. Retrieved 2020年03月31日, from 京都大学OCW Web site: https://ocw.kyoto-u.ac.jp/ja/international-conference/09/how-microbes-evolve-to-dodge-the-membrane. ...
Avhandlingar om ANTIMICROBIAL PEPTIDES. Sök bland 89654 avhandlingar från svenska högskolor och universitet på Avhandlingar.se.
Shop Probable antibacterial peptide polyprotein ELISA Kit, Recombinant Protein and Probable antibacterial peptide polyprotein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Scientists from Novozymes have now in collaboration with researchers at University of Bonn, Aalborg and others found the mechanism by which plectasin, an anti-microbial peptide, kills bacteria that cause severe infections in humans.
BMAP-28: C-terminal domain of a cathelicidin, a myeloid antimicrobial peptide precursor; BMAP-28 stands for bovine myeloid antimicrobial peptide of 28 residues; amino acid sequence in first source; GenBank X97609
The eye is a complex organ and has developed a unique means to fight off germs. The eye has its own separate immune function to tackle a potential infection, which is called immune privilege. Research has since learned exactly how these peptides went about killing their enemies although the results are better placed in a Clive Barker novel -- AMPs manifest the well-known concept of death by a million paper cuts ...
PLOS Pathogens publishes Open Access research and commentary that significantly advance the understanding of pathogens and how they interact with host organisms. Get Started ...
Reactome is pathway database which provides intuitive bioinformatics tools for the visualisation, interpretation and analysis of pathway knowledge.
Isms of action on target microBIBS39 biological activity organism than that of existing antibiotics. Antimicrobial peptides (AMPs) play an important role as a
Volume 50: Synthesis and Antimicrobial Screening 6-Aryl-2-(Amino/Methoxy)-4-[(4′-Difluoro Methoxy) (3′-Hydroxy) Phenyl] Nicotinonitrile
A peroxyacid antimicrobial concentrate and use composition is provided comprising a C.sub.1 to C.sub.4 peroxycarboxylic acid, and a C.sub.6 to C.sub.18 peroxyacid. The combination of these acids produces a synergistic effect, providing a much more potent biocide than can be obtained by using these c ...
cAMP stringently regulates human cathelicidin antimicrobial peptide expression in the mucosal epithelial cells by activating cAMP-response element-binding protein, AP-1, and inducible cAMP early repressor
The antimicrobial peptide LL-37 belongs to the cathelicidin family and is the first amphipathic α-helical peptide isolated from human. LL-37 is considered to play an important role in the first line of defence against local infection and systemic invasion of pathogens at sites of inflammation and wounds. Understanding its mode of action may assist in the development of antimicrobial agents mimicking those of the human immune system. In vitrostudies revealed that LL-37 is cytotoxic to both bacterial and normal eukaryotic cells. To gain insight into the mechanism of its non-cell-selective cytotoxicity, we synthesized and structurally and functionally characterized LL-37, its N-terminal truncated form FF-33, and their fluorescent derivatives (which retained structure and activity). The results showed several differences, between LL-37 and other native antimicrobial peptides, that may shed light on its in vivoactivities. Most interestingly, LL-37 exists in equilibrium between monomers and oligomers ...
Mechanical ventilation (MV) of patients can cause damage to bronchoalveolar epithelium, leading to a sterile inflammatory response, infection and in severe cases sepsis. Limited knowledge is available on the effects of MV on the innate immune defense system in the human lung. In this study, we demonstrate that cyclic stretch of the human bronchial epithelial cell lines VA10 and BCi NS 1.1 leads to down-regulation of cathelicidin antimicrobial peptide (CAMP) gene expression. We show that treatment of VA10 cells with vitamin D3 and/or 4-phenyl butyric acid counteracted cyclic stretch mediated down-regulation of CAMP mRNA and protein expression (LL-37). Further, we observed an increase in pro-inflammatory responses in the VA10 cell line subjected to cyclic stretch. The mRNA expression of the genes encoding pro-inflammatory cytokines IL-8 and IL-1β was increased after cyclic stretching, where as a decrease in gene expression of chemokines IP-10 and RANTES was observed. Cyclic stretch enhanced oxidative
Objectives: To investigate the in vitro antifungal activity of the structurally different cathelicidin peptides SMAP-29, BMAP-27, BMAP-28, protegrin-1 (PG-1) and indolicidin. Methods: The in vitro antifungal and fungicidal activities of these antimicrobial peptides were respectively assessed via MIC determinations and killing kinetics assays. The effects of the peptides on membrane permeabilization and morphology were evaluated by flow cytometry, intracellular ATP release measurements and scanning electron microscopy. Results: All five peptides showed a potent but differential antifungal activity against more than 70 clinical isolates belonging to over 20 different species of pathogenic fungi; some of which are resistant to amphotericin B and azoles. The MIC values of the peptides ranged between 0.5 and 32 mM, with PG-1 being the most effective and having the widest spectrum of activity. Filamentous fungi were instead found to be scarcely susceptible to the action of these cathelicidin peptides. ...
ABSTRACT: BACKGROUND: Transcription of the cathelicidin antimicrobial peptide (CAMP) gene is induced by binding of the bioactive form of vitamin D, 1,25-dihydroxyvitamin D, to the vitamin D receptor. Significant levels of the protein hCAP18/LL-37 are found in the blood and may protect against infection and/or sepsis. We hypothesized that serum vitamin D levels may modulate the circulating levels of hCAP18. Only three studies have shown a positive correlation between circulating 25-hydroxyvitamin D and hCAP18 levels. Here we provide additional evidence for such a correlation in healthy, middle-aged adults. FINDINGS: Serum levels of 25-hydroxyvitamin D [25(OH)D] and plasma levels of hCAP18 were determined in 19 healthy middle-aged (mean of 50.1 years) adult men and women. Plasma hCAP18 concentrations correlated with serum 25(OH)D concentrations in subjects with 25(OH)D levels 32 ng/ml (r = 0.19, p = 0.63). CONCLUSIONS: We conclude that plasma hCAP18 levels correlate with serum 25(OH)D levels in ...
Antimicrobial peptides are multifunctional in innate immunity and wound repair of multicellular organisms. We were the first to discover that histatins, a family of salivary antimicrobial peptides, enhance epithelial cell migration, suggesting a role in oral wound healing. It is unknown whether histatins display innate-immunity activities, similar to other antimicrobial peptides such as LL-37. Therefore, we compared the effect of Histatin-2 and LL-37 on several activities within the context of wound healing and innate immunity. We found that Histatin-2 enhances fibroblast migration, but only weakly induces proliferation. LL-37 enhances both fibroblast migration and proliferation, but only at a narrow concentration optimum (approximately 1 μm). At higher concentrations LL-37 causes cell death, whereas Histatin-2 is not cytotoxic. Both peptides do not alter fibroblast-to-myofibroblast differentiation. Histatin-2 does not alter interleukin-8 (IL-8) expression and lipopolysaccharide (LPS)-elevated ...
The production of antimicrobial peptides is essential for protection against a wide variety of microbial pathogens and plays an important role in the pathogenesis of several diseases. The mechanisms responsible for expression of antimicrobial peptides are incompletely understood, but a role for vitamin D as a transcriptional inducer of the antimicrobial peptide cathelicidin has been proposed. We show that 1,25-dihydroxyvitamin D3 (1,25-D3) acts together with parathyroid hormone (PTH), or the shared amino-terminal domain of PTH-related peptide (PTHrP), to synergistically increase cathelicidin and immune defense. Administration of PTH to mouse skin decreased susceptibility to skin infection by group A Streptococcus. Mice on dietary vitamin D3 restriction that responded with an elevation in PTH have an increased risk of infection if they lack 1,25-D3. These results identify PTH/PTHrP as a variable that serves to compensate for inadequate vitamin D during activation of antimicrobial peptide ...
Growth factors, comprising diverse protein and peptide families, are involved in a multitude of developmental processes, including embryogenesis, angiogenesis, and wound healing. Here we show that peptides derived from HB-EGF, amphiregulin, hepatocyte growth factor, PDGF-A and PDGF-B, as well as various FGFs are antimicrobial, demonstrating a previously unknown activity of growth factor-derived peptides. The peptides killed the Gram-negative bacteria Escherichia coli, Pseudomonas aeruginosa, and the Gram-positive Bacillus subtilis, as well as the fungus Candida albicans. Several peptides were also active against the Gram-positive S. aureus. Electron microscopy analysis of peptide-treated bacteria, paired with analysis of peptide effects on liposomes, showed that the peptides exerted membrane-breaking effects similar to those seen after treatment with the "classical" human antimicrobial peptide LL-37. Furthermore, HB-EGF was antibacterial per se, and its epitope GKRKKKGKGLGKKRDPCLRKYK retained ...
As described previously,17 the BMP-RE controls human hepcidin promoter transcriptional activity under steady state-condition. In order to assess the effect of the heterozygous nc.-153 C,T mutation on basal hepcidin gene expression, plasmid constructs containing the -1024 bp of the human hepcidin promoter with the wild type or the mutated BMP-RE inserted among the luciferase gene (-1024/BMP-RE wt Hep/Luc and -1024/BMP-RE mut Hep/Luc) were transfected in HepG2 cells. Luciferase activity from the -1024/BMP-RE mut Hep/Luc construct was about 2.4 fold reduced compared to the luciferase activity from the plasmid construct containing the wild type BMP-RE (Figure 1, lanes 1-2), demonstrating that this mutation decreased hepcidin transcriptional activity in basal condition. As this BMP-RE mediates the induction of hepcidin gene expression in response to BMP,17 HepG2 cells were transfected with the -1024/BMP-RE wt or mutated Hep/Luc plasmid constructs and were treated with either BMP9 or BMP4 in order to ...
Gombart AF , Borregaard N , Koeffler HP . Department of Medicine, Division of Hematology/Oncology, Cedars-Sinai Medical Center, David Geffen School of Medicine at UCLA, Los Angeles, California 90048, USA. [email protected] The innate immune system of mammals provides a rapid response to repel assaults from numerous infectious agents including bacteria, viruses, fungi, and parasites. A major component of this system is a diverse combination of cationic antimicrobial peptides that include the alpha- and beta-defensins and cathelicidins. In this study, we show that 1,25-dihydroxyvitamin D3 and three of its analogs induced expression of the human cathelicidin antimicrobial peptide (CAMP) gene. This induction was observed in acute myeloid leukemia (AML), immortalized keratinocyte, and colon cancer cell lines, as well as normal human bone marrow (BM) -derived macrophages and fresh BM cells from two normal individuals and one AML patient. The induction occurred via a consensus vitamin D response ...
LL37 antibody, Cathelicidin antimicrobial antibody for the specific detection of Cathelicidin antimicrobial peptide by immunohistochemistry / western blot using Mouse monoclonal to human Cathelicidin (CAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): OSX12 clone, OSC00155G, CAP-18 monocolnal antibody, LL37 monocolnal antibody, CAMP monocolnal antibody, CRAMP monocolnal antibody, Cathelicidin monocolnal antibody, LL-37 antibody
Peptides , Antimicrobial and Related Peptides , LL17-32; This peptide is an active segment of LL-37, a peptide derived from the C-terminal domain of human cathelicidin antimicrobial peptide. It has been reported that the LL17-32 peptide exhibits reversal effect on ABCG2-mediated multidrug resistance in cancer cell lines.; FKRIVQRIKDFLRNLV; H-Phe-Lys-Arg-Ile-Val-Gln-Arg-Ile-Lys-Asp-Phe-Leu-Arg-Asn-Leu-Val-OH
en] Animals are colonized by coevolved bacterial communities, which contribute to the hosts health. This commensal microbiota is often highly specific to its host-species, inferring strong selective pressures on the associated microbes. Several factors, including diet, mucus composition, and the immune system have been proposed as putative determinants of host-associated bacterial communities. Here we report that species-specific antimicrobial peptides account for different bacterial communities associated with closely related species of the cnidarian Hydra. Gene family extensions for potent antimicrobial peptides, the arminins, were detected in four Hydra species, with each species possessing a unique composition and expression profile of arminins. For functional analysis, we inoculated arminin-deficient and control polyps with bacterial consortia characteristic for different Hydra species and compared their selective preferences by 454 pyrosequencing of the bacterial microbiota. In contrast ...
We have synthesized 39 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] analogs having two side chains attached to carbon-20 (Gemini) with various modifications and compared their anticancer activities. Five structure-function rules emerged to identify analogs with enhanced anticancer activity. One of these active analogs, BXL-01-0126, was more potent than 1,25(OH)2D3 in mediating 50% clonal inhibition of cancer cell growth. Murine studies found that BXL-01-0126 and 1,25(OH)2D3 had nearly the same potency to raise serum calcium levels. Taken together, BXL-01-0126 when compared to 1,25(OH)2D3 has greater anticancer potency, but similar toxicity causing hypercalcemia. We focused on the effect of these compounds on the stimulation of expression of human cathelicidin antimicrobial peptide (CAMP) whose gene has a vitamin D response element in its promoter. Expression of CAMP mRNA and protein increased in a dose-response fashion after exposure of acute myeloid leukemia (AML) cells to the Gemini analog, ...
The Gram-negative bacterium Neisseria meningitidis is a transient commensal of the human nasopharynx, but occasionally causes life-threatening disease. During colonisation of its niche, N. meningitidis has to overcome innate immune defences, including the expression of antimicrobial peptides (AMPs). Meningococcal resistance to the host defence peptide LL-37 was investigated in Papers I and II. The polysaccharide capsule and lipopolysaccharide (LPS) were found to increase LL-37 resistance by inhibiting peptide binding to the bacteria. Further, N. meningitidis responded to sub-lethal doses of LL-37 by an increase in capsule biosynthesis. Intriguingly, adhesion to epithelial cells and tissues protected N. meningitidis from physiological concentrations of LL-37 and two other helical peptides. The protective effect was mediated by RhoA- and Cdc42-dependent host cell signalling and cholesterol-rich membrane microdomains. The host epithelium thus seems to play an active role in AMP ...
The ability of epithelial cells to sense the external environment and communicate this information to the local immune system, thereby initiating appropriate responses, is essential for the maintenance of health and prevention of the development of chronic inflammatory diseases. Our studies have shown that an oral probiotic commensal strain of S. salivarius is able to inhibit inflammatory responses in human bronchial epithelial cells by downregulating the NF-κB pathway. This is consistent with an emerging paradigm that indicates the downregulation of epithelial immune responses by commensal bacteria (8, 13, 24, 38, 48). Not only did S. salivarius K12 inhibit baseline synthesis of IL-8, but it also suppressed IL-8 secretion when cells were stimulated with pathogenic P. aeruginosa, Salmonella serovar Typhimurium flagellin, or the immunomodulatory host defense peptide LL-37. Most previous studies have focused on IL-8 and IL-6 responses, but here it was demonstrated that this commensal bacterium ...
Targeting the BMP pathway: as the BMP pathway plays a key role in stimulating hepcidin transcription, sequestration of BMP ligands could decrease hepcidin expression. Heparin, a glycosaminoglycan widely used as an anticoagulant, has long been known to bind BMPs.68 Poli and colleagues demonstrated that heparin inhibited hepcidin expression in hepatic cell lines as well as in mice.69 Daily injections in mice for seven days (50 mg/kg/d) decreased hepcidin mRNA expression and SMAD phosphorylation, increased serum iron and reduced spleen iron concentration. In 5 patients treated with low molecular weight heparin to prevent deep vein thrombosis, serum hepcidin concentration decreased by 80-85% within 2-5 days after the start of the treatment. Concurrently, increased serum iron levels and transferrin saturation were noted in all 5 patients. Although the safety profile of heparin is well understood, its anticoagulant activity hinders its wider application to iron-restricted disorders. To address this, ...
This resistance is adaptive in that it depends on the biofilm growth state and although many explanations have been provided to explain it, it is likely that changes in gene and/or protein expression in the biofilm state explain why organisms become resistant.. Intriguingly despite this problem, not a single antibiotic has been developed for treating biofilms. We have started to address this using as templates the cationic host defence (antimicrobial) peptides, which are produced by virtually all organisms as a major part of their innate defences against infection. They are a key component of innate immunity and have multiple mechanisms that enable them to deal with infections and inflammation, including selective modulation of innate immunity, activity against bacterial biofilms (the cause of 65% of all human infections) and direct antimicrobial activity. We made the breakthrough observation that human peptide LL-37 was able to inhibit Pseudomonas aeruginosa biofilms at one sixteenth of its MIC ...
Peptides , Antimicrobial and Related Peptides , rCRAMP; This peptide is a rat homologue of the human cathelicidin LL-37. Rat cathelicidin-related antimicrobial peptide (rCRAMP) was identified in granulocytes, thymus, testis, lung, mouth mucosa, tongue, oesophagus, colon, caecum and small intestine. The rCRAMP peptide is present in specific CNS regions and may play a role in the innate immunity of the CNS. rCRAMP exhibits pro-healing activity in stomach.; GLVRKGGEKFGEKLRKIGQKIKEFFQKLALEIEQ; H-Gly-Leu-Val-Arg-Lys-Gly-Gly-Glu-Lys-Phe-Gly-Glu-Lys-Leu-Arg-Lys-Ile-Gly-Gln-Lys-Ile-Lys-Glu-Phe-Phe-Gln-Lys-Leu-Ala-Leu-Glu-Ile-Glu-Gln-OH
Moreover the human α-Defensin human neutrophil peptide 1 was revealed to show anti-HIV activity. HNP-one inhibits the binding of the virus to its coreceptor , the endocytosis of the virus into the target cell as well as the release of the HIV-genome from the endosome into the cytoplasm. Even so HNP-one did not inhibit the endocytosis of Influenza A virus displaying some selectivity of the AMPs in their tropism. These final results evidently demonstrate that defensins not only screen antimicrobial activity but in addition are lively from viruses as nicely.Bactericidal/permeability-increasing protein belongs to the class of AMPs. In distinction to the over mentioned defensins BPI owing to the 55 kDa molecular measurement of the protein is structurally significantly a lot more intricate than the peptides, which are in the selection of 3-five kDa. The BPI protein loved ones contains of far more than ten associates but only BPI alone displays a powerful antimicrobial exercise. BPI functions ...
Using our CAMP discovery process and only 100 μl of alligator plasma, we have previously identified five novel antimicrobial peptides from A. mississippiensis that exhibit antibacterial activity [8]. Our bioprospecting-based process provides a unique access to the antimicrobial peptidome, and is a significant advance in the effort to identify novel antimicrobial peptides in nature. In this study, we present detailed characterization of the structure and function of three alligator plasma-derived peptides: Apo5, Apo6, and A1P. We demonstrated that Apo5, Apo6, and A1P are potent antimicrobial peptides that extend their efficacy against multi-drug resistant and clinically relevant pathogens, such as A. baumannii. The two peptides Apo5 and Apo6 are both derived from a predicted apolipoprotein C-1 in A. mississippiensis. Apolipoproteins bind lipids; apolipoprotein C-1 in particular is known to bind phospholipids and is a marker of apoptosis [11, 12, 43, 44]. Apo6 is a smaller derivative of Apo5; ...
Le, J; Dam, Q; Schweizer, M et al. (2016) Effects of vancomycin versus nafcillin in enhancing killing of methicillin-susceptible Staphylococcus aureus causing bacteremia by human cathelicidin LL-37. Eur J Clin Microbiol Infect Dis 35:1441-7 ...
Rossi , D C , Munoz , J E , Carvalho , D D , Belmonte , R , Faintuch , B , Borelli , P , Miranda , A , Taborda , C P & Daffre , S 2012 , Therapeutic use of a cationic antimicrobial peptide from the spider Acanthoscurria gomesiana in the control of experimental candidiasis BioMed Central Microbiology , vol 12 , 28 . DOI: 10.1186/1471-2180-12- ...
Doctoral Recruitment- Host Defense Peptides in the Pathogenesis of Inflammatory Intestinal Diseases. Job Summary. A Ph.D. student position awarded by the prestigious Eyes High Doctoral Recruitment Scholarship funding at Univ. of Calgary is available to provide 4 years of funding for either domestic or international student. The research aim of this student will be to investigate the production and antimicrobial and immune modulatory functions of host defense cathelicidin peptides innately secreted by the intestinal epithelium and surrounding neutrophils in intestinal homeostasis and disease. These peptides are important components of the epithelial innate immune system in humans and livestock animals, with still unknown roles controlling infection and mitigating inflammatory responses. The goal is to advance cathelicidins as natural and effective alternatives to current treatments for enterocolitis that could reduce the use of conventional antibiotics. Our approach includes studies conducted in ...
An azurophilic granule is a cellular object readily stainable with a Romanowsky stain. In white blood cells and hyperchromatin, staining imparts a burgundy or merlot coloration. Neutrophils in particular are known for containing azurophils loaded with a wide variety of anti-microbial defensins that fuse with phagocytic vacuoles. Azurophils may contain myeloperoxidase, phospholipase A2, acid hydrolases, elastase, defensins, neutral serine proteases, bactericidal/permeability-increasing protein, lysozyme, cathepsin G, proteinase 3, and proteoglycans. Azurophil granules are also known as "primary granules". Furthermore, the term "azurophils" may refer to a unique type of cells, identified only in reptiles. These cells are similar in size to so-called heterophils with abundant cytoplasm that is finely to coarsely granular and may sometimes contain vacuoles. Granules may impart a purplish hue to the cytoplasm, particularly to the outer region. Occasionally, azurophils are observed with vacuolated ...
TY - JOUR. T1 - Antimicrobial polymethacrylates synthesized as mimics of tryptophan-rich cationic peptides. AU - Locock, Katherine E.S.. AU - Michl, Thomas D.. AU - Stevens, Natalie. AU - Hayball, John D.. AU - Vasilev, Krasimir. AU - Postma, Almar. AU - Griesser, Hans J.. AU - Meagher, Laurence. AU - Haeussler, Matthias. PY - 2014/4/15. Y1 - 2014/4/15. N2 - This study describes a facile and high yielding route to two series of polymethacrylates inspired by the naturally occurring, tryptophan-rich cationic antimicrobial polymers. Appropriate optimization of indole content within each gave rise to polymers with high potency against Staphylococcus epidermidis (e.g., PGI-3 minimum inhibitory concentration (MIC) = 12 μg/mL) and the methicillin-resistant strain of Staphylococcus aureus (e.g., PGI-3 MIC = 47 μg/mL) with minimal toxicity toward human red blood cells. Future work will be directed toward understanding the cooperative roles that the cationic and indole pendant groups have for the ...
...CORVALLIS Ore. Scientists have just identified a new reason why some...New research at Oregon State University has discovered that curcumin ...This cathelicidin antimicrobial peptide or CAMP is part of what help...Prior to this it was known that CAMP levels were increased by vitamin...,Like,curry?,New,biological,role,identified,for,compound,used,in,ancient,medicine,biological,biology news articles,biology news today,latest biology news,current biology news,biology newsletters
Complete information for CAMP gene (Protein Coding), Cathelicidin Antimicrobial Peptide, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
The study describes an elegant tool for what has been a frustrating problem. Of particular interest to us, this study shows that Aβ dimers have striking structural similarities to the dimeric forms of the antimicrobial peptides human NP2, horseshoe crab tachystatin B, and mouse α-defensin. We recently reported on the antimicrobial activity of Aβ, and our newest data suggest dimerization is a key event in turning relatively inactive Aβ monomers into forms that can attack bacteria. This is not a phenomenon restricted to Aβ. It has been known for some time that oligomerization has an important role in the action and targeting of a number of antimicrobial proteins (AMPs), including the archetypal antimicrobial peptide LL-37 which can form oligomers, fibrils, and birefringent amyloid (albeit, LL-37 amyloid has only been observed in vitro to date). Oligomerization is also a key mechanism for antimicrobial protein-mediated agglutination and inactivation of viral particles.. Despite this central ...
Antimicrobial peptides represent the first-line host defence against microbial pathogens and an essential component of innate immunity. They have received growing interest because of their potential use as therapeutic antibiotics. Due to the fact that most antimicrobial peptides are toxic to prokaryotic host cells, they are currently often produced by chemical synthesis. However, this is too costly for them to be used when large quantities of antimicrobial peptides are required for investigations and clinical trials. Thus, the convenience and cost efficiencies of bacterial production of antimicrobial peptides have become a bottleneck problem.. As an important group of antimicrobial peptides human ß-defensins are cationic peptides with 38-47 amino acid residues showing three strands of anti-parallel β-sheets that provide a compact small structure [1,2]. We describe an optimized strategy for recombinant expression of hBD-1 and its mutants in Escherichia coli, to efficiently produce milligram ...
Three novel antimicrobial peptides (AMPs), named panurgines (PNGs), were isolated from the venom of the wild bee Panurgus calcaratus. The dodecapeptide of the sequence LNWGAILKHIIK-NH2 (PNG-1) belongs
We participate in the revolution in fluorescence microscopy of biological systems. It is now possible to measure the spatial distribution of proteins and DNA loci with 30-nm precision in live cells and to track their motion in real time. The result is an unprecedented, high resolution view of biological structure and activity. Areas of current interest include: (1) The motion and spatial distribution of GFP-labeled species in live E. coli cells. Species of interest include RNA polymerase, ribosomes, architectural proteins, and specific DNA loci. The transcription/translation machinery (ribosomes, the nucleoid, and RNAP) all exhibit a remarkable level of spatial organization. (2) The time-resolved attack of antimicrobial agents on single bacterial cell membranes. Examples include LL-37, a human antimicrobial peptide, and synthetic random copolymers designed by the Gellman group. Simultaneous two-color imaging of the antimicrobial and cytoplasmic or periplasmic GFP yields unprecedented insight ...
OmpA Binding Mediates the Effect of Antimicrobial Peptide LL-37 on Acinetobacter baumannii. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Hepcidin is a key regulator of systemic iron homeostasis. Hepcidin deficiency induces iron overload, whereas hepcidin excess induces anemia. Mutations in the gene encoding hemojuvelin (HFE2, also known as HJV) cause severe iron overload and correlate with low hepcidin levels, suggesting that hemojuvelin positively regulates hepcidin expression. Hemojuvelin is a member of the repulsive guidance molecule (RGM) family, which also includes the bone morphogenetic protein (BMP) coreceptors RGMA and DRAGON (RGMB). Here, we report that hemojuvelin is a BMP coreceptor and that hemojuvelin mutants associated with hemochromatosis have impaired BMP signaling ability. Furthermore, BMP upregulates hepatocyte hepcidin expression, a process enhanced by hemojuvelin and blunted in Hfe2-/- hepatocytes. Our data suggest a mechanism by which HFE2 mutations cause hemochromatosis: hemojuvelin dysfunction decreases BMP signaling, thereby lowering hepcidin expression. ...
In the results presented here, we find that an oral infection with wild-type Salmonella serovar Typhimurium results in a significant decrease in innate host defense effector molecules of the small intestine. The decreases in cryptdin and lysozyme expression are the first evidence that Paneth cell antimicrobial expression can be altered by bacterial infection with an intestinal pathogen in vivo. Salmonella-induced decrease of Paneth cell antimicrobial peptide mRNA and protein levels may be one of its survival mechanisms in the intestinal lumen and required for subsequent invasion.. The ability of intestinal pathogens to downregulate host antimicrobials is not restricted to Salmonella. Shigella flexneri infection is able to decrease the expression of human α-defensin-1 and LL-37 in colonic epithelial cell lines and human colonic biopsy specimens (19). These findings suggest that this regulation requires the Shigella virulence plasmid DNA alone, even in the absence of live bacteria (19). S. ...
Human being mesenchymal stromal cells (MSC) possess immunosuppressive and antimicrobial results that are partly mediated by the tryptophan-catabolizing enzyme indoleamine-2,3-dioxygenase (IDO). in the treatment of GvHD in transplant individuals, we recommend that individuals planned for MSC therapy should go through comprehensive evaluation for an energetic CMV disease and get CMV-directed antiviral therapy prior to the administration of MSC. 1. Intro Human being multipotent mesenchymal stromal cells (MSC), known for their multilineage difference potential, have pleiotropic immunosuppressive features that are mediated by phrase of the tryptophan-catabolizing enzyme indoleamine-2 partially,3-dioxygenase (IDO) [1C4]. Upon arousal with inflammatory cytokines, MSC show broad-spectrum antimicrobial effector features aimed against BMS-536924 different relevant pathogens medically, and these results are reliant on IDO and/or the antimicrobial peptide LL-37 [5, 6]. These dual immunosuppressive and ...
Hepcidin is a liver-derived hormone with a key role in iron homeostasis. In addition to iron, it is regulated by inflammation and hypoxia, although mechanisms of hypoxic regulation remain unclear. In hepatocytes, hepcidin is induced by bone morphogenetic proteins (BMPs) through a receptor complex requiring hemojuvelin (HJV) as a co-receptor. Type II transmembrane serine proteinase (TMPRSS6) antagonizes hepcidin induction by BMPs by cleaving HJV from the cell membrane. Inactivating mutations in TMPRSS6 lead to elevated hepcidin levels and consequent iron deficiency anemia. Here we demonstrate that TMPRSS6 is up-regulated in hepatic cell lines by hypoxia and by other activators of hypoxia-inducible factor (HIF). We show that TMPRSS6 expression is regulated by both HIF-1α and HIF-2α. This HIF-dependent up-regulation of TMPRSS6 increases membrane HJV shedding and decreases hepcidin promoter responsiveness to BMP signaling in hepatocytes. Our results reveal a potential role for TMPRSS6 in hepcidin
Gene transfer for the cure of ß-thalassemia and sickle cell anemia. Beta-thalassemia and sickle cell anemia (SCD) represent the most common hemoglobinopathies caused, respectively, by deficient production or alteration of the hemoglobin beta-chain subunit. We are generating gene-delivering tools for the cure of these disorders. Our approaches are based on achieving therapeutic levels of corrective genes (beta-globin and/or gamma globin) following gene transfer or use of drugs that modify the splicing of beta-globin RNA mutant forms. We are also developing new strategies to introduce genetically modified cells into the bone marrow with minimal conditioning.. Erythropoiesis and iron metabolism in beta-thalassemia, hemochromatosis and polycythemia vera. Progressive iron overload is the most salient and ultimately fatal complication of beta-thalassemia. The hepatic peptide hepcidin limits iron absorption and recycling, degrading the iron exporter ferroportin at the level of enterocytes and ...
Two partial mRNA sequences predicted to encode anti-lipopolysaccharide factors (ALFs) were identified among expressed sequence tags generated from the American lobster Homarus americanus and complete cDNA sequences were obtained from library clones. Comparison of the translated amino acid sequences to those publicly available confirmed similarity to arthropod anti-lipopolysaccharide factors. Both protein sequences, designated ALFHa-1 and ALFHa-2, contained an N-terminal signal peptide and two Show moreTwo partial mRNA sequences predicted to encode anti-lipopolysaccharide factors (ALFs) were identified among expressed sequence tags generated from the American lobster Homarus americanus and complete cDNA sequences were obtained from library clones. Comparison of the translated amino acid sequences to those publicly available confirmed similarity to arthropod anti-lipopolysaccharide factors. Both protein sequences, designated ALFHa-1 and ALFHa-2, contained an N-terminal signal peptide and two ...
Chlorpyrifos (CPF) is commonly used for agricultural and domestic applications, and its contamination is widely detected in environmental media, and in a broad spectrum of field crops, fruits and vegetables. CPF exposure causes many adverse effects on human health including hepatoxicity, neurotoxicity and endocrine disruption. However, few studies have been conducted thus far to investigate the potential influence of CPF exposure on iron metabolism at concentrations that do not trigger significant cell death. Iron metabolism is concertedly governed by the hepcidin-ferroportin axis, where hepcidin is the central hormone involved in the regulation of iron absorption and release, while ferroportin is the only known iron exporter that functions by iron egress from cells. In the present study, we demonstrated that CPF treatment at a non-toxic concentration greatly enhanced ferroportin gene transcription in human macrophage THP-1 cells. CPF significantly inhibited hepcidin expression in human ...

Improvement of outer membrane-permeabilizing and lipopolysaccharide- binding activities of an antimicrobial cationic peptide by...Improvement of outer membrane-permeabilizing and lipopolysaccharide- binding activities of an antimicrobial cationic peptide by...

... binding activities of an antimicrobial cationic peptide by C-terminal modification",. abstract = "Antimicrobial cationic ... binding activities of an antimicrobial cationic peptide by C-terminal modification. Antimicrobial Agents and Chemotherapy, 38( ... binding activities of an antimicrobial cationic peptide by C-terminal modification. In: Antimicrobial Agents and Chemotherapy. ... binding activities of an antimicrobial cationic peptide by C-terminal modification, Antimicrobial Agents and Chemotherapy, vol ...
more infohttps://portal.sahmriresearch.org/en/publications/improvement-of-outer-membrane-permeabilizing-and-lipopolysacchari

Structure and organization of the human antimicrobial peptide LL-37 in phospholipid membranes: relevance to the molecular basis...Structure and organization of the human antimicrobial peptide LL-37 in phospholipid membranes: relevance to the molecular basis...

Acyl carrier protein is a bacterial cytoplasmic target of cationic antimicrobial peptide LL-37 Biochem J (August, 2015) ... The antimicrobial peptide LL-37 belongs to the cathelicidin family and is the first amphipathic α-helical peptide isolated from ... Lipid-binding and antimicrobial properties of synthetic peptides of bovine apolipoprotein A-II Biochem J (August, 1999) ... Structure and organization of the human antimicrobial peptide LL-37 in phospholipid membranes: relevance to the molecular basis ...
more infohttps://portlandpress.com/biochemj/article-abstract/341/3/501/36526/Structure-and-organization-of-the-human

Cyclic mechanical stretch down-regulates cathelicidin antimicrobial peptide expression and activates a pro-inflammatory...Cyclic mechanical stretch down-regulates cathelicidin antimicrobial peptide expression and activates a pro-inflammatory...

... of the human bronchial epithelial cell lines VA10 and BCi NS 1.1 leads to down-regulation of cathelicidin antimicrobial peptide ... results indicate that cyclic stretch may differentially modulate innate immunity by down-regulation of antimicrobial peptide ... These cationic polypeptides are categorized into: (1) smaller processed peptides such as cathelicidins and defensins and (2) ... Antimicrobial peptides in the airway.. Current Topics in Microbiology and Immunology 306:153-182 ...
more infohttps://peerj.com/articles/1483/

Browsing Publications by Subject Antimicrobial Cationic PeptidesBrowsing Publications by Subject "Antimicrobial Cationic Peptides"

... 0-9. A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P. Q. R. ... Antimicrobial Medicines Consumption (‎AMC)‎ Network. AMC data 2011-2014  World Health Organization. Regional Office for Europe ... This report sets out and analyses data on antimicrobial medicines consumption (‎AMC)‎ collected from non-European Union ...
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按主题Antimicrobial Cationic Peptides浏览Publications按主题"Antimicrobial Cationic Peptides"浏览Publications

Antimicrobial Medicines Consumption (‎AMC)‎ Network. AMC data 2011-2014  World Health Organization. Regional Office for Europe ... This report sets out and analyses data on antimicrobial medicines consumption (‎AMC)‎ collected from non-European Union ... "Antimicrobial Cationic Peptides"浏览Publications. 0-9. A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P. Q. R. S. T. U. V. W. X. Y ...
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Alternative mechanisms of action of cationic antimicrobial peptides on bacteria.  - PubMed - NCBIAlternative mechanisms of action of cationic antimicrobial peptides on bacteria. - PubMed - NCBI

Cationic antimicrobial peptides are a novel type of antibiotic offering much potential in the treatment of microbial-related ... This article presents an updated review of how cationic antimicrobial peptides are able to affect bacterial killing, with a ... Alternative mechanisms of action of cationic antimicrobial peptides on bacteria.. Hale JD1, Hancock RE. ... While the dogma for the mechanism of action of antimicrobial peptides against bacteria is believed to be through pore formation ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/18039080?dopt=Abstract

Regulation of Salmonella typhimurium virulence gene expression by cationic antimicrobial peptides.  - PubMed - NCBIRegulation of Salmonella typhimurium virulence gene expression by cationic antimicrobial peptides. - PubMed - NCBI

Cationic antimicrobial peptides (CAMP) represent a conserved and highly effective component of innate immunity. During ... Regulation of Salmonella typhimurium virulence gene expression by cationic antimicrobial peptides.. Bader MW1, Navarre WW, ... indicating that the PhoP/PhoQ system can sense sublethal concentrations of cationic antimicrobial peptides. Growth of S. ... We further demonstrate that growth of S. typhimurium in low doses of the alpha-helical peptide C18G induces resistance to CAMP ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/14507376?dopt=Abstract

Nanomechanical response of bacterial cells to cationic antimicrobial peptides - Soft Matter (RSC Publishing)Nanomechanical response of bacterial cells to cationic antimicrobial peptides - Soft Matter (RSC Publishing)

The effectiveness of antimicrobial compounds can be easily screened, however their mechanism of action is much more difficult ... Nanomechanical response of bacterial cells to cationic antimicrobial peptides Shun Lu,a Grant Walters,a Richard Parga and John ... Nanomechanical response of bacterial cells to cationic antimicrobial peptides S. Lu, G. Walters, R. Parg and J. R. Dutcher, ... unique insight into the kinetics and mechanism of action of antimicrobial peptides on bacteria. ...
more infohttp://pubs.rsc.org/en/content/articlelanding/2014/sm/c3sm52801d/unauth

Frontiers | Covalent modification of a ten-residue cationic antimicrobial peptide with levofloxacin | ChemistryFrontiers | Covalent modification of a ten-residue cationic antimicrobial peptide with levofloxacin | Chemistry

As such, there has been growing interest in cationic antimicrobial peptides (CAMPs) and their therapeutic applications. ... molecule antibiotics with favorable physicochemical properties to CAMPs could be a promising strategy for enhancing peptide ... As such, there has been growing interest in cationic antimicrobial peptides (CAMPs) and their therapeutic applications. ... peptide. ]. total. −. [. peptide. ]. PO4buffer. ). /. (. [. peptide. ]. PO4buffer. ). ). =. log. (. [. peptide. ]. octanol. /. ...
more infohttps://www.frontiersin.org/articles/10.3389/fchem.2014.00071/full

Frontiers | Synergy Pattern of Short Cationic Antimicrobial Peptides Against Multidrug-Resistant Pseudomonas aeruginosa |...Frontiers | Synergy Pattern of Short Cationic Antimicrobial Peptides Against Multidrug-Resistant Pseudomonas aeruginosa |...

Antimicrobial peptides (AMPs) cannot only kill MDR bacteria, but also can be used synergistically with conventional antibiotics ... Single amino acid substitutions within the peptides can have a very strong effect on the ability to synergize, making it ... A high number of combinations between peptides and polymyxin B, erythromycin, and tetracycline were found to be synergistic. ... A high number of combinations between peptides and Polymyxin B, Erythromycin and Tetracycline were found to be synergistic. ...
more infohttps://www.frontiersin.org/articles/10.3389/fmicb.2019.02740/full

Strategies for Recombinant Expression of Small, Highly Disulphide- Bonded, Cationic Antimicrobial Peptides | BenthamScienceStrategies for Recombinant Expression of Small, Highly Disulphide- Bonded, Cationic Antimicrobial Peptides | BenthamScience

Strategies for Recombinant Expression of Small, Highly Disulphide- Bonded, Cationic Antimicrobial Peptides. Author(s): A. L. ... Title: Strategies for Recombinant Expression of Small, Highly Disulphide- Bonded, Cationic Antimicrobial Peptides ... Keywords:Disulphide bond, refolding, recombinant expression, antimicrobial peptide, inclusion body. Abstract: Expression of two ... Expression of two recombinant hepcidin homologues from Atlantic salmon, Salmo salar, characterization of their antimicrobial ...
more infohttp://www.eurekaselect.com/83227/article

Gradual pediocin PA-1 resistance in Enterococcus faecalis confers cross-protection to diverse pore-forming cationic...Gradual pediocin PA-1 resistance in Enterococcus faecalis confers cross-protection to diverse pore-forming cationic...

... identification of new alternatives has increased interest in diverse populations of potent cationic... ... Enterococcus faecalis pediocin PA-1 cationic antimicrobial peptides resistance permeability barrier This is a preview of ... Peschel A, Sahl HG (2006) The co-evolution of host cationic antimicrobial peptides and microbial resistance. Nat Rev Microbiol ... McBride SM, Sonenshein AL (2011) The dlt operon confers resistance to cationic antimicrobial peptides in Clostridium difficile ...
more infohttps://link.springer.com/article/10.1007%2Fs13213-014-0912-1

Antiendotoxin activity of cationic peptide antimicrobial agents. | Infection and ImmunityAntiendotoxin activity of cationic peptide antimicrobial agents. | Infection and Immunity

Antiendotoxin activity of cationic peptide antimicrobial agents.. M Gough, R E Hancock, N M Kelly ... Antiendotoxin activity of cationic peptide antimicrobial agents. Message Subject (Your Name) has forwarded a page to you from ... We describe here two small cationic peptides, MBI-27 and MBI-28, which have both antiendotoxic and antibacterial activities in ... We had previously demonstrated that these peptides bind to LPS with an affinity equivalent to that of polymyxin B. Consistent ...
more infohttps://iai.asm.org/content/64/12/4922?ijkey=4d1c45e5b34e43d0e18385216dedf89ed4973051&keytype2=tf_ipsecsha

New core-shell hydrogel particles for the separation and discovery of cationic antimicrobial peptides (CAMPs)New core-shell hydrogel particles for the separation and discovery of cationic antimicrobial peptides (CAMPs)

... polymerization for the harvest of low-abundance cationic antimicrobial peptides ... New core-shell hydrogel particles for the separation and discovery of cationic antimicrobial peptides (CAMPs). Y. Zhu, P. Russo ... polymerization for the harvest of low-abundance cationic antimicrobial peptides (CAMPs) from complex biological samples, such ... These core-shell hydrogel particles were able to pull down cationic peptides from alligator serum spiked with known CAMPs, ...
more infohttp://www.techconnectworld.com/World2015/a.html?i=573

Collectins and cationic antimicrobial peptides of the respiratory epit by Branka Grubor, David K. Meyerholz et al."Collectins and cationic antimicrobial peptides of the respiratory epit" by Branka Grubor, David K. Meyerholz et al.

Collectins and cationic antimicrobial peptides are antimicrobial components of the pulmonary innate immune system produced by ... The purpose of this review is to discuss antimicrobial innate immune elements within the respiratory tract of healthy and ... Collectins and cationic antimicrobial peptides are antimicrobial components of the pulmonary innate immune system produced by ... Grubor, Branka; Meyerholz, David K.; and Ackermann, Mark R., "Collectins and cationic antimicrobial peptides of the respiratory ...
more infohttps://lib.dr.iastate.edu/vpath_pubs/32/

Antimicrobial Cationic Peptides | CTDAntimicrobial Cationic Peptides | CTD

Amphipathic Cationic Antimicrobial Peptides , Antimicrobial Peptides, Cationic , Cationic Antimicrobial Peptides , Cationic ... Antimicrobial Cationic , Peptides, Cationic Antimicrobial , Proteins, Microbicidal Cationic Definition Small cationic peptides ... Peptides, Antimicrobial , Cationic Proteins, Microbicidal , Microbicidal Cationic Proteins , Peptides, ... Chemicals ← Amino Acids, Peptides, and Proteins ← PeptidesAntimicrobial Cationic Peptides Top ↑ Descendants Antimicrobial ...
more infohttp://ctdbase.org/detail.go?type=chem&acc=D023181

The Mechanisms of Action of Cationic Antimicrobial Peptides Refined by Novel Concepts from Biophysical Investigations |...The Mechanisms of Action of Cationic Antimicrobial Peptides Refined by Novel Concepts from Biophysical Investigations |...

... biophysical and structural investigations on how these peptides interact with membranes can still bear surprises and add new ... Smart M, Rajagopal A, Liu WK, Ha BY (2017) Opposing effects of cationic antimicrobial peptides and divalent cations on ... Hadley EB, Hancock RE (2010) Strategies for the discovery and advancement of novel cationic antimicrobial peptides. Curr Top ... Aisenbrey C., Marquette A., Bechinger B. (2019) The Mechanisms of Action of Cationic Antimicrobial Peptides Refined by Novel ...
more infohttps://rd.springer.com/chapter/10.1007/978-981-13-3588-4_4

De Novo Generation of Cationic Antimicrobial Peptides: Influence of Length and Tryptophan Substitution on Antimicrobial...De Novo Generation of Cationic Antimicrobial Peptides: Influence of Length and Tryptophan Substitution on Antimicrobial...

De Novo Generation of Cationic Antimicrobial Peptides: Influence of Length and Tryptophan Substitution on Antimicrobial ... De Novo Generation of Cationic Antimicrobial Peptides: Influence of Length and Tryptophan Substitution on Antimicrobial ... De Novo Generation of Cationic Antimicrobial Peptides: Influence of Length and Tryptophan Substitution on Antimicrobial ... De Novo Generation of Cationic Antimicrobial Peptides: Influence of Length and Tryptophan Substitution on Antimicrobial ...
more infohttps://aac.asm.org/content/49/1/316?ijkey=06266a89980a616429a30bf68b0e72d75c3c10ef&keytype2=tf_ipsecsha

Lipid topology and electrostatic interactions underpin lytic activity of linear cationic antimicrobial peptides in membranes  -...Lipid topology and electrostatic interactions underpin lytic activity of linear cationic antimicrobial peptides in membranes -...

Linear cationic antimicrobial peptides are a diverse class of molecules that interact with a wide range of cell membranes. Many ... Lipid topology and electrostatic interactions underpin lytic activity of linear cationic antimicrobial peptides in membranes ... Lipid topology and electrostatic interactions underpin lytic activity of linear cationic antimicrobial peptides in membranes. ... We propose a topological model for linear antimicrobial peptide activity based on the increase in membrane strain caused by the ...
more infohttp://eprints.gla.ac.uk/145693/

High Specific Selectivity and Membrane-Active Mechanism of Synthetic Cationic Hybrid Antimicrobial Peptides Based on the...High Specific Selectivity and Membrane-Active Mechanism of Synthetic Cationic Hybrid Antimicrobial Peptides Based on the...

In this study, we designed a series of synthetic cationic hybrid antimicrobial peptides based on the peptide FV7 combined with ... In conclusion, we designed a series of synthetic cationic hybrid antimicrobial peptides based on the peptide FV7 combined with ... FV-LL Is the Optimal Hybrid Peptide Compared with the Parental Peptides and Other Peptides in the Antimicrobial Activities. The ... a series of effective hybrid cationic antimicrobial peptides were designed that combined the parental peptide FV7 with ...
more infohttp://pubmedcentralcanada.ca/pmcc/articles/PMC5343874/

A Novel Defensin-Like Peptide Associated with Two  Other New Cationic Antimicrobial Peptides in Transcriptome  of the Iranian...A Novel Defensin-Like Peptide Associated with Two Other New Cationic Antimicrobial Peptides in Transcriptome of the Iranian...

... and some antimicrobial peptides (AMPs) have been found in the venom gland of scorpions. Therefore, the discovery of new anti- ... Scorpion venom is a source of bioactive peptides, ... Peptide Associated with Two Other New Cationic Antimicrobial ... Introduction: Scorpion venom is a source of bioactive peptides, and some antimicrobial peptides (AMPs) have been found in the ... Results: MeuVAP-6 and meuAP-18-1 are non-disulphide-bridged antimicrobial peptides, while meuPep34 is a cysteine-rich defensin- ...
more infohttp://ibj.pasteur.ac.ir/browse.php?a_id=1845&slc_lang=en&printcase=1&hbnr=1&hmb=1

Cationic Antimicrobial Peptides and Their Multifunctional Role in the Immune System - Critical Reviews™ in Immunology, Volume...Cationic Antimicrobial Peptides and Their Multifunctional Role in the Immune System - Critical Reviews™ in Immunology, Volume...

The antimicrobial activity of these peptides has been studie... ... Many species of life contain cationic antimicrobial peptides as ... Such cationic antimicrobial peptides can also act in synergy with host molecules, such as other cationic peptides and proteins ... Many species of life contain cationic antimicrobial peptides as components of their immune systems. The antimicrobial activity ... Cationic Antimicrobial Peptides and Their Multifunctional Role in the Immune System. Monisha G. Scott Department of ...
more infohttp://www.dl.begellhouse.com/fr/journals/2ff21abf44b19838,17f890ef49d056ce,17938e4314593376.html?sgstd=1

Synthetic cationic antimicrobial peptides bind with their hydrophobic parts to drug site II of human serum albumin | BMC...Synthetic cationic antimicrobial peptides bind with their hydrophobic parts to drug site II of human serum albumin | BMC...

... but is also surprisingly able to bind positively charged peptides. Understanding of how short cationic antimicrobial peptides ... The binding of a selection of short synthetic cationic antimicrobial peptides (CAPs) to human albumin with binding affinities ... We suggest that albumin binding should be taken into careful consideration in antimicrobial peptide studies, as the systemic ... of albumin-peptide complexes reported here provide detailed insight into how albumin can bind short cationic peptides. The ...
more infohttps://bmcstructbiol.biomedcentral.com/articles/10.1186/1472-6807-14-4

Intracellular biomass flocculation as a key mechanism of rapid bacterial killing by cationic, amphipathic antimicrobial...Intracellular biomass flocculation as a key mechanism of rapid bacterial killing by cationic, amphipathic antimicrobial...

... amphipathic antimicrobial peptides and peptoids, Scientific Reports" on DeepDyve, the largest online rental service for ... "Intracellular biomass flocculation as a key mechanism of rapid bacterial killing by cationic, ... Mechanism of interaction of different classes of cationic antimicrobial peptides with planar bilayers and with the cytoplasmic ... The antimicrobial peptides lactoferricin B and magainin 2 cross over the bacterial cytoplasmic membrane and reside in the ...
more infohttps://www.deepdyve.com/lp/springer_journal/intracellular-biomass-flocculation-as-a-key-mechanism-of-rapid-PbiUTLfII9

Defensins are cationic antimicrobial peptides that contribute to regulations of sponsor - Factor VIII inhibitors in hemophilia ADefensins are cationic antimicrobial peptides that contribute to regulations of sponsor - Factor VIII inhibitors in hemophilia A

Defensins are cationic antimicrobial peptides that contribute to regulations of sponsor. healthweeks , January 9, 2018 ... Defensins are cationic antimicrobial peptides that contribute to regulations of sponsor cell function also. Human being ... The reaction was carried out for 2 h and labeled peptide was re-purified by HPLC. The molecular mass of the peptides was ... Neutrophil Peptide 1-4 and Human being Defensin 5 and -6. Human being Neutrophil Peptides (HNP1-3) differ from each additional ...
more infohttp://healthweeks.com/2018/01/09/defensins-are-cationic-antimicrobial-peptides-that-contribute-to-regulations-of-sponsor/
  • In this study, we investigated the mechanisms of actions of melittin and two synthetic peptides, CEME (a cecropin-melittin hybrid) and CEMA, against gram-negative bacteria. (sahmriresearch.org)
  • To examine this possibility we used polarized attenuated total reflectance Fourier-transform infrared spectroscopy and found that the peptide is predominantly α-helical and oriented nearly parallel with the surface of zwitterionic-lipid membranes. (portlandpress.com)
  • Antimicrobial cationic peptides have been discovered in many different organisms and often possess a broad range of activity. (sahmriresearch.org)
  • These data collectively indicated that these peptides all cross the outer membrane by the self-promoted uptake pathway and that CEMA is the peptide most effective at accessing this pathway. (sahmriresearch.org)
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