Hepatitis, Infectious Canine: A contagious disease caused by canine adenovirus (ADENOVIRUSES, CANINE) infecting the LIVER, the EYE, the KIDNEY, and other organs in dogs, other canids, and bears. Symptoms include FEVER; EDEMA; VOMITING; and DIARRHEA.Antigens, Viral: Substances elaborated by viruses that have antigenic activity.Dog Diseases: Diseases of the domestic dog (Canis familiaris). This term does not include diseases of wild dogs, WOLVES; FOXES; and other Canidae for which the heading CARNIVORA is used.Antigens: Substances that are recognized by the immune system and induce an immune reaction.Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.Liver Neoplasms: Tumors or cancer of the LIVER.Prognosis: A prediction of the probable outcome of a disease based on a individual's condition and the usual course of the disease as seen in similar situations.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.Microscopy, Electron: Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.Liver Diseases: Pathological processes of the LIVER.Herpesvirus 4, Human: The type species of LYMPHOCRYPTOVIRUS, subfamily GAMMAHERPESVIRINAE, infecting B-cells in humans. It is thought to be the causative agent of INFECTIOUS MONONUCLEOSIS and is strongly associated with oral hairy leukoplakia (LEUKOPLAKIA, HAIRY;), BURKITT LYMPHOMA; and other malignancies.Capsid: The outer protein protective shell of a virus, which protects the viral nucleic acid.Laboratories: Facilities equipped to carry out investigative procedures.Epstein-Barr Virus Infections: Infection with human herpesvirus 4 (HERPESVIRUS 4, HUMAN); which may facilitate the development of various lymphoproliferative disorders. These include BURKITT LYMPHOMA (African type), INFECTIOUS MONONUCLEOSIS, and oral hairy leukoplakia (LEUKOPLAKIA, HAIRY).Infectious Mononucleosis: A common, acute infection usually caused by the Epstein-Barr virus (HERPESVIRUS 4, HUMAN). There is an increase in mononuclear white blood cells and other atypical lymphocytes, generalized lymphadenopathy, splenomegaly, and occasionally hepatomegaly with hepatitis.Capsid Proteins: Proteins that form the CAPSID of VIRUSES.Antibodies, Viral: Immunoglobulins produced in response to VIRAL ANTIGENS.Diagnostic Techniques and Procedures: Methods, procedures, and tests performed to diagnose disease, disordered function, or disability.Menu PlanningParaneoplastic Cerebellar Degeneration: Cerebellar degeneration associated with a remote neoplasm. Clinical manifestations include progressive limb and GAIT ATAXIA; DYSARTHRIA; and NYSTAGMUS, PATHOLOGIC. The histologic type of the associated neoplasm is usually carcinoma or lymphoma. Pathologically the cerebellar cortex and subcortical nuclei demonstrate diffuse degenerative changes. Anti-Purkinje cell antibodies (anti-Yo) are found in the serum of approximately 50% of affected individuals. (Adams et al., Principles of Neurology, 6th ed, p686)Paraneoplastic Syndromes: In patients with neoplastic diseases a wide variety of clinical pictures which are indirect and usually remote effects produced by tumor cell metabolites or other products.T-Lymphocytes, Cytotoxic: Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.Cerebellar Diseases: Diseases that affect the structure or function of the cerebellum. Cardinal manifestations of cerebellar dysfunction include dysmetria, GAIT ATAXIA, and MUSCLE HYPOTONIA.Clonal Anergy: Functional inactivation of T- or B-lymphocytes rendering them incapable of eliciting an immune response to antigen. This occurs through different mechanisms in the two kinds of lymphocytes and can contribute to SELF TOLERANCE.Paraneoplastic Syndromes, Nervous System: Degenerative or inflammatory conditions affecting the central or peripheral nervous system that develop in association with a systemic neoplasm without direct invasion by tumor. They may be associated with circulating antibodies that react with the affected neural tissue. (Intern Med 1996 Dec;35(12):925-9)Cytotoxicity, Immunologic: The phenomenon of target cell destruction by immunologically active effector cells. It may be brought about directly by sensitized T-lymphocytes or by lymphoid or myeloid "killer" cells, or it may be mediated by cytotoxic antibody, cytotoxic factor released by lymphoid cells, or complement.Autoantigens: Endogenous tissue constituents that have the ability to interact with AUTOANTIBODIES and cause an immune response.Lambert-Eaton Myasthenic Syndrome: An autoimmune disease characterized by weakness and fatigability of proximal muscles, particularly of the pelvic girdle, lower extremities, trunk, and shoulder girdle. There is relative sparing of extraocular and bulbar muscles. CARCINOMA, SMALL CELL of the lung is a frequently associated condition, although other malignancies and autoimmune diseases may be associated. Muscular weakness results from impaired impulse transmission at the NEUROMUSCULAR JUNCTION. Presynaptic calcium channel dysfunction leads to a reduced amount of acetylcholine being released in response to stimulation of the nerve. (From Adams et al., Principles of Neurology, 6th ed, pp 1471)Mice, Inbred C57BLGranzymes: A family of serine endopeptidases found in the SECRETORY GRANULES of LEUKOCYTES such as CYTOTOXIC T-LYMPHOCYTES and NATURAL KILLER CELLS. When secreted into the intercellular space granzymes act to eliminate transformed and virus-infected host cells.B-Lymphocytes: Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.Receptors, Antigen, B-Cell: IMMUNOGLOBULINS on the surface of B-LYMPHOCYTES. Their MESSENGER RNA contains an EXON with a membrane spanning sequence, producing immunoglobulins in the form of type I transmembrane proteins as opposed to secreted immunoglobulins (ANTIBODIES) which do not contain the membrane spanning segment.Serine Endopeptidases: Any member of the group of ENDOPEPTIDASES containing at the active site a serine residue involved in catalysis.Lymphocyte Activation: Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.B-Lymphocyte Subsets: A classification of B-lymphocytes based on structurally or functionally different populations of cells.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

Reverse transcription-nested polymerase chain reaction for detecting p40 RNA of Borna disease virus, without risk of plasmid contamination. (1/10399)

Several methods for the detection of Borna disease virus (BDV) RNA have been reported, one being the reverse transcription-nested polymerase chain reaction (RT-nested PCR) method. However, due to the possibility of contamination of the cloned DNA in a reaction tube, false-positive results might be obtained by RT-nested PCR. To detect only BDV RNA without anxiety of contamination, we developed an RT-nested PCR system using "mRNA selective PCR kit". Using this system, cDNA of BDV p40 in the plasmid (up to 5 x 10(7) molecules) was not amplified. BDV specific sequence was amplified from total RNA (more than 50 pg) of MDCK/BDV cells, which were persistently infected with BDV. These results indicate that this mRNA selective RT-nested PCR system can specifically amplify target RNA as distinguished from plasmid contaminated.  (+info)

Inhibition of Pichinde virus replication by actinomycin D. (2/10399)

The yields of Pichinde virus, a member of the arenavirus group, were markedly inhibited when infected BHK 21 cells were incubated in the presence of 0.4 to 4 mug/ml of actinomycin D. Maximal inhibition was observed when actinomycin D was added after the adsorption of virus to cultures; however, addition of drug as late as 12 h after infection reduced the 24 h yield by 50%. Virus antigen synthesis, as measured by complement fixation and immunodiffusion, was not dramatically reduced by actinomycin D. The expression of virus antigens on the surface of infected cells was greater on cells treated with actinomycin D than on untreated cells. Putative defective particles with a density of Pichinde virus were not detected in fluids of cultures incubated with actinomycin D and 3H-amino acids. Actinomycin D appears to inhibit Pichinde virus late in the replicative cycle. The observations raise the possibility that the drug inhibits the synthesis of proteins of the host cell membrane which are required for virus maturation.  (+info)

The clinical utility of CMV surveillance cultures and antigenemia following bone marrow transplantation. (3/10399)

At our institution, the cytomegalovirus (CMV) prophylaxis protocol for allogeneic bone marrow transplant (BMT) recipients who are CMV-seropositive or receive marrow from a CMV-seropositive donor consists of a surveillance bronchoscopy approximately 35 days posttransplant. Patients with a positive surveillance bronchoscopy for CMV receive pre-emptive ganciclovir. In order to determine the utility of other screening methods for CMV, we prospectively performed weekly CMV antigenemia, and blood, urine and throat cultures from time of engraftment to day 120 post-BMT in 126 consecutive patients. Pre-emptive ganciclovir was given to 11/81 patients (13.6%) because of a positive surveillance bronchoscopy for CMV. Results of CMV blood, urine and throat cultures and the antigenemia assay done prior to or at the time of the surveillance bronchoscopy were analyzed for their ability to predict the bronchoscopy result. The antigenemia test had the highest positive and negative predictive values (72% and 96%, respectively). The ability of these tests to predict CMV disease was evaluated in the 70 patients with a negative surveillance bronchoscopy who did not receive pre-emptive ganciclovir. Of 19 cases of active CMV disease, CMV antigenemia was positive in 15 patients (79%) a mean of 34 days preceding symptoms. Blood cultures were positive in 14/19 patients (74%) a mean of 31 days before onset of disease. CMV antigenemia is useful for predicting the surveillance bronchoscopy result, and also predicts the development of CMV disease in the majority of patients missed by the surveillance bronchoscopy.  (+info)

A new picornavirus isolated from bank voles (Clethrionomys glareolus). (4/10399)

A previously unknown picornavirus was isolated from bank voles (Clethrionomys glareolus). Electron microscopy images and sequence data of the prototype isolate, named Ljungan virus, showed that it is a picornavirus. The amino acid sequences of predicted Ljungan virus capsid proteins VP2 and VP3 were closely related to the human pathogen echovirus 22 (approximately 70% similarity). A partial 5' noncoding region sequence of Ljungan virus showed the highest degree of relatedness to cardioviruses. Two additional isolates were serologically and molecularly related to the prototype.  (+info)

Immune response to the immunodominant epitope of mouse hepatitis virus is polyclonal, but functionally monospecific in C57Bl/6 mice. (5/10399)

Mutations in an immunodominant CD8 CTL epitope (S-510-518) are selected in mice persistently infected with the neurotropic JHM strain of mouse hepatitis virus. These mutations abrogate recognition by T cells harvested from the infected CNS in direct ex vivo cytotoxicity assays. Previous reports have suggested that, in general, an oligoclonal, monospecific T cell response contributes to the selection of CTL escape mutants. Herein, we show that, in MHV-JHM-infected mice, the CD8 T cell response after intraperitoneal infection is polyclonal and diverse. This diverse response was shown to include both polyclonal and oligoclonal components. The polyclonal data were shown to fit a logarithmic distribution. With regard to specificity, we used a panel of peptide analogues of epitope S-510-518 and spleen-derived CD8 T cell lines to determine why only a subset of possible mutations was selected in persistently infected mice. At a given position in the epitope, the mutations identified in in vivo isolates were among those that resulted in the greatest loss of recognition. However, not all such mutations were selected, suggesting that additional factors must contribute to selection in vivo. By extrapolation of these results to the persistently infected CNS, they suggest that the selection of CTL escape mutants requires the presence of a monospecific T cell response but also show that this response need not be oligoclonal.  (+info)

Immune surveillance against a solid tumor fails because of immunological ignorance. (6/10399)

Many peripheral solid tumors such as sarcomas and carcinomas express tumor-specific antigens that can serve as targets for immune effector T cells. Nevertheless, overall immune surveillance against such tumors seems relatively inefficient. We studied immune surveillance against a s.c. sarcoma expressing a characterized viral tumor antigen. Surprisingly, the tumor cells were capable of inducing a protective cytotoxic T cell response if transferred as a single-cell suspension. However, if they were transplanted as small tumor pieces, tumors readily grew. Tumor growth correlated strictly with (i) failure of tumor cells to reach the draining lymph nodes and (ii) absence of primed cytotoxic T cells. Cytotoxic T cells were not tolerant or deleted because a tumor antigen-specific cytotoxic T cell response was readily induced in lymphoid tissue by immunization with virus or with tumor cells even in the presence of large tumors. Established tumors were rejected by vaccine-induced effector T cells if effector T cells were maintained by prolonged or repetitive vaccination, but not by single-dose vaccination. Thus, in addition to several other tumor-promoting parameters, some antigenic peripheral sarcomas-and probably carcinomas-may grow not because they anergize or tolerize tumor-specific T cells, but because such tumors are immunologically dealt with as if they were in a so-called immunologically privileged site and are ignored for too long.  (+info)

Development of a Western blot assay for detection of bovine immunodeficiency-like virus using capsid and transmembrane envelope proteins expressed from recombinant baculovirus. (7/10399)

A 120-amino-acid polypeptide selected from the transmembrane protein region (tTM) and the major capsid protein p26 of bovine immunodeficiency-like virus (BIV) were expressed as fusion proteins from recombinant baculoviruses. The antigenic reactivity of both recombinant fusion proteins was confirmed by Western blot with bovine and rabbit antisera to BIV. BIV-negative bovine sera and animal sera positive for bovine syncytial virus and bovine leukemia virus failed to recognize the recombinant fusion proteins, thereby showing the specificity of the BIV Western blot. One hundred and five bovine serum samples were tested for the presence of anti-BIV antibodies by the recombinant protein-based Western blot and a reference Western blot assay using cell culture-derived virions as test antigens. There was a 100% concordance when the p26 fusion protein was used in the Western blot. However, the Western blot using the tTM fusion protein as its test antigen identified four BIV-positive bovine sera which had tested negative in both the p26 recombinant-protein-based and the reference Western blot assays. This resulted in the lower concordance of 96.2% between the tTM-protein-based and reference Western blot assays. The results of this study showed that the recombinant p26 and tTM proteins can be used as test antigens for the serodetection of BIV-infection in animals.  (+info)

Human serum antibodies to a major defined epitope of human herpesvirus 8 small viral capsid antigen. (8/10399)

The major antibody-reactive epitope of the small viral capsid antigen (sVCA) of human herpesvirus 8 (HHV-8) was defined by use of overlapping peptides. Strong IgG reactivity was found among approximately 50% of 44 human immunodeficiency virus-positive or -negative patients with Kaposi's sarcoma and 13 subjects who were seropositive by immunofluorescence assay (IFA) for the latent HHV-8 nuclear antigen. Only 1 of 106 subjects seronegative for both lytic and latent HHV-8 antigens and 10 of 81 subjects IFA-seropositive only for the lytic HHV-8 antigen had strong IgG reactivity to this epitope. Among 534 healthy Swedish women, only 1.3% were strongly seropositive. Comparison of the peptide-based and purified sVCA protein-based ELISAs found 55% sensitivity and 98% specificity. However, only 1 of 452 serum samples from healthy women was positive in both tests. In conclusion, the defined sVCA epitope was a specific, but not very sensitive, serologic marker of active HHV-8 infection. Such infection appears to be rare among Swedish women, even with sexual risk-taking behavior.  (+info)

*Epstein-Barr virus viral-capsid antigen

The Epstein-Barr virus viral-capsid antigen (EBV-VCA) is the viral protein that forms the viral capsid of the Epstein-Barr ... It is the antigen targeted by anti-VCA antibodies.[clarification needed] Such antibodies can be used in serology to diagnose ...

*Orthoreovirus

Viral antigen can be detected by performing an assay. A serological assay can also be performed on the sample to look for virus ... The viral protein gyanyltransferase λ2 is responsible for capping the viral mRNA. Mammalian orthoreovirus mRNA transcripts have ... "Orthoreovirus". Viral Zone. Retrieved 1 October 2014. editors, Michael G. Rossmann, Venigalla B. Rao, (2012). Viral molecular ... "Viral Zone". Anonymous. "Orthoreovirus". ViralZone. Retrieved 1 October 2014. Anonymous. "Orthoreovirus". Viral Zone. Retrieved ...

*Hepatitis A vaccine

Each adult dose contains 500 U of viral antigen. The pediatric dose contains 250 U of viral antigen. Vaqta: made by Merck. ... Each adult dose contains 1440 ELISA units of viral antigen adsorbed on aluminium hydroxide (0.5 mg Al). The pediatric (child) ... doses contain half the amount of viral antigen and aluminium. Healive: made by Sinovac. Inactivated hepatitis A virus cultured ... "Hepatitis, Viral, Type A". Travelers' Health: Yellow Book (CDC). Archived from the original on 28 March 2007. Retrieved 12 ...

*Tick-borne encephalitis

In such cases a marker for TBE infection is elevated IFN-g in CSF.[citation needed] Viral antigen can usually be found in brain ... Tick-borne encephalitis (TBE) is a viral infectious disease involving the central nervous system. The disease most often ... Tickborne encephalitis at Centers for Disease Control and Prevention (CDC) Factsheet from Viral Special Pathogens Branch at the ...

*Shope papilloma virus

Fluorescent antibody study identified the locations of viral antigens in wild rabbit papillomas. They were present only in the ... In domesticated rabbits, the viral antigens were present in much smaller quantity in only superficial, keratinized layers. The ... Viral replication proteins E1 and E2 are also required to form the papilloma and keep the episomal viral genome replication low ... E4 proteins are expressed, and viral DNA amplification starts in the differentiated cells. Following this, the L1 and L2 viral ...

*Yellow fever

Liver biopsy can verify inflammation and necrosis of hepatocytes and detect viral antigens. Because of the bleeding tendency of ... The sfRNAs are a result of incomplete degradation of the viral genome by the exonuclease and are important for viral ... Other viral hemorrhagic fevers, such as Ebola virus, Lassa virus, Marburg virus, and Junin virus, must be excluded as cause. ... The UTR contains PKS3 pseudoknot structure which serves as a molecular signal to stall the exonuclease and is the only viral ...

*Japanese encephalitis

Viral antigen can be shown in tissues by indirect fluorescent antibody staining. Infection with Japanese encephalitis confers ... The outer envelope is formed by envelope protein and is the protective antigen. It aids in entry of the virus into the inside ... NS3 is a putative helicase, and NS5 is the viral polymerase. It has been noted that Japanese encephalitis infects the lumen of ... Japanese encephalitis (JE) is the leading cause of viral encephalitis in Asia, with up to 70,000 cases reported annually. Case- ...

*Polioencephalitis

Blood tests can be done to detect antibodies against viral antigens and foreign proteins. Virus isolation is the most sensitive ... Polioencephalitis is a viral infection of the brain, causing inflammation within the grey matter of the brain stem. The virus ... Experiments on transgenic mice are investigating the initial sites of viral replication in the host and how the virus moves ... "Polioencephalitis and the brain Fatigue Generator Model of Post-Viral Fatigue Syndromes". J. Of Chronic Fatigue Syndrome. 2 (2- ...

*Rabies in Haiti

There do exist other methods for viral antigen detection that may merit study. In 2013, the CDC and its partners began an ... Rabies is a viral disease that exists in Haiti and throughout the world. It often causes fatal inflammation of the brain in ...

*Dengue fever

PCR and viral antigen detection are more accurate in the first seven days. In 2012 a PCR test was introduced that can run on ... where the cell's protein synthesis apparatus produces new viral proteins that replicate the viral RNA and begin to form viral ... This can be done by virus isolation in cell cultures, nucleic acid detection by PCR, viral antigen detection (such as for NS1) ... The first approach is inhibition of the viral RNA-dependent RNA polymerase (coded by NS5), which copies the viral genetic ...

*TAP1

"Presentation of viral antigen controlled by a gene in the major histocompatibility complex". Nature. 345 (6274): 449-52. doi: ... Transporter associated with Antigen Processing 1 is a protein that in humans is encoded by the TAP1 gene. The membrane- ... Gobin SJ, Wilson L, Keijsers V, Van den Elsen PJ (1997). "Antigen processing and presentation by human trophoblast-derived cell ... WHO Nomenclature Committee for factors of the HLA system". Tissue Antigens. 39 (4): 161-73. doi:10.1111/j.1399-0039.1992. ...

*Herpesviridae

Viral proteins inhibit TAP preventing the MHC from picking up a viral antigen peptide. This prevents proper folding of the MHC ... allowing viral DNA to migrate to the cell nucleus. Within the nucleus, replication of viral DNA and transcription of viral ... During symptomatic infection, infected cells transcribe lytic viral genes. In some host cells, a small number of viral genes ... As discussed above, one way is by a viral chemokine homolog such as IL-10. Another mechanism to down regulate MHCs is to encode ...

*CCDC85B

DIPA interacts with the viral antigen, HDAg, and can affect HDV replication in vitro. CCDC85B has been shown to interact with: ... "A cellular homolog of hepatitis delta antigen: implications for viral replication and evolution". Science. 274 (5284): 90-4. ... Long M, de Souza SJ, Gilbert W (May 1997). "Delta-interacting protein A and the origin of hepatitis delta antigen". Science. ... Delta-interacting protein A (DIPA), a cellular gene product, has been found to have homology to hepatitis delta virus antigen ( ...

*Avian nephritis virus

For definitive diagnosis the virus or viral antigens must be demonstrated in the affected tissue(s). Diagnostic techniques used ...

*Julianna Lisziewicz

It is a new immunization strategy that uses dendritic cells to increase viral antigen production. DermaVir contains a plasmid ... These complementary DNA pieces can bind to the HIV viral mRNA and prevent the HIV virus from replicating itself. The use of ... Langerhans cells are a natural agent of antigen release. They are the precursors of dendritic cells in the skin and mucosa. ... Another investigation Lisziewicz is involved with is how human leukocyte antigen (HLA) genes regulate immune responses. This ...

*Bovine viral diarrhea

Antigen ELISA and rtPCR are currently the most frequently performed tests to detect virus or viral antigen. Individual testing ... PIs act as viral reservoirs and are the principal source of viral infection but transiently infected animals and contaminated ... Bovine viral diarrhea (BVD) or bovine viral diarrhoea (UK English), and previously referred to as bovine virus diarrhoea (BVD ... Following viral entry and contact with the mucosal lining of the mouth or nose, replication occurs in epithelial cells. BVDV ...

*Daniel A. Portnoy

Ikonomidis, G.; Paterson, Y.; Kos, F. J.; Portnoy, D. A. (1994-12-01). "Delivery of a viral antigen to the class I processing ... "Induction of a cellular immune response to a foreign antigen by a recombinant Listeria monocytogenes vaccine". Journal of ...

*Pepscan

Geysen M, et al (1984). Use of peptide synthesis to probe viral antigens for epitopes to a resolution of a single amino acid. ...

*José Esparza

Drake, S; Schaffer, PA; Esparza, J; Mayor, HD (Jul 1974). "Complementation of adeno-associated satellite viral antigens and ... By this stage Esparza had attained hands-on command of many aspects of viral research techniques (i.e., virus-isolation, ... From 1986 to 2014 he worked continuously as a viral vaccine expert and senior public health adviser for international health ... During his undergraduate teenage years, Esparza teamed up with some of his teachers in viral research studies designed to ...

*ISCOM

"Qualitative T-Helper Responses to Multiple Viral Antigens Correlate with Vaccine-Induced Immunity to Simian/Human ... Moreover, the technology is highly efficient; its long-lasting immune responses allow reduction of the antigen dose. Typically ... If required, however, the antigen can also be incorporated into the structure. Immunologic adjuvant Immune system Immunology ... Since the ISCOM-Matrix is simply mixed with the antigen post-manufacturing, it offers great production advantages and ...

*Porcine parvovirus

The first unequivocal evidence of viral replication is the appearance of nascent viral antigen in the nucleus (Fig. 2A). In at ... Viral antigen is detected in the cytoplasm of cells soon after infection if the inoculum contains a high titer of virus and ... 5). Virus and viral antigen are widely distributed in tissues of infected embryos and their placentas, and it is probable that ... Viral antigen is especially concentrated in lymphoid tissues (Fig. 3A, B). Many pigs, irrespective of age or sex, have a ...

*Glioblastoma

Association of viral antigen in low grade gliomas". Journal of Clinical Virology. 46 (1): 37-42. doi:10.1016/j.jcv.2009.05.011 ... McFaline-Figueroa, JR; Wen, PY (February 2017). "The Viral Connection to Glioblastoma". Current infectious disease reports. 19 ...

*Gammaherpesvirinae

... are characteristically latent in lymphocytes and drive the proliferation that requires the expression of latent viral antigens ... Although the first ORF in these gammaherpesviruses have oncogenic potential, other viral genes may also play a role in viral ... At the left end of each viral genome are located ORFs encoding distinct transforming proteins.The Gammaherpes viral genes are ... the viral episomes are partitioned to daughter cells. This provides continuous existence of the viral genome within the host ...

*Transverse myelitis

One major theory posits that immune-mediated inflammation is present as the result of exposure to a viral antigen.[citation ... Viral association of transverse myelitis could result from the infection itself or from the response to it. Bacterial causes ... Viral infections, include herpes simplex and herpes zoster, cytomegalovirus, and Epstein-Barr. Flavivirus infections include ...

*Peter K. Vogt

"Localization of infectious virus and viral antigen in chick fibroblasts during successive stages of infection with Rous sarcoma ... At the beginning of his scientific career, Vogt studied mechanisms of retroviral cell entry and the role of viral surface ... He defined related groups of viral surface proteins and their corresponding receptors on the cell surface. During his time in ... His research focuses on retroviruses and viral and cellular oncogenes. Vogt received his undergraduate education in biology at ...

*Phage display

Instead, one could cleave in a section between the bead and the antigen to elute. Since the pIII is intact it does not matter ... so that they are displayed on the surface of the viral particle. The protein displayed corresponds to the genetic sequence ... The technique is also used to determine tumour antigens (for use in diagnosis and therapeutic targeting) and in searching for ... novel expression vectors that display cloned antigens on the virion surface". Science. 228 (4705): 1315-7. Bibcode:1985Sci... ...
Expression of viral antigen in target tissues of mice 3-7 days after in vivo infection.Spleen and bone marrow cells were isolated from mice infected by the s.
The E.Coli derived recombinant artificial mosaic protein contains two epitopes from C-end associated in one molecule from the HHV-4 p18 regions.
BioAssay record AID 288762 submitted by ChEMBL: Inhibition of TPA-induced Epstein-Barr virus early antigen activation assessed as EBV-EA induction in Raji cells at 3.2 nM after 48 hrs relative to TPA.
BioAssay record AID 289353 submitted by ChEMBL: Inhibition of TPA-induced Epstein-Barr virus early antigen activation in Raji cells at 16 nmol relative to control.
Epstein-Barr virus early antigen: synthesized before or in the absence of viral-progeny DNA replication & present only in infected cells
Thank you for sharing this Journal of Virology article.. NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.. ...
Over the past 15 years, a number of observations have culminated in the localization of viral antigens in liver tissue by the use of routine histology, immunohistochemistry, electron microscopy (EM),...
The role of Epstein-Barr virus (EBV) early antigen diffuse component (EA-D) and its relationship with EBV DNA polymerase in EBV genome-carrying cells are unclear, EBV-specified DNA polymerase was purified in a sequential manner from Raji cells treated with phorbol-12,13-dibutyrate and n-butyrate by phosphocellulose, DEAE-cellulose, double-stranded DNA-cellulose, and blue Sepharose column chromatography. Four polypeptides with molecular masses of 110,000, 100,000, 55,000, and 49,000 daltons were found to be associated with EBV-specified DNA polymerase activity. A monoclonal antibody which could neutralize the EBV DNA polymerase activity was prepared and found to recognize 55,000- and 49,000-dalton polypeptides. An EA-D monoclonal antibody, R3 (G. R. Pearson, V. Vorman, B. Chase, T. Sculley, M. Hummel, and E. Kieff, J. Virol. 47:183-201, 1983), was also able to recognize these same two polypeptides associated with EBV DNA polymerase activity. It was concluded that EBV EA-D polypeptides, as ...
Summary The mol. wt. of the polymorphic Epstein-Barr virus (EBV) nuclear antigen (EBNA) molecule (EBNA 1) encoded by the BamHI K fragment of the EBV DNA has been determined in 14 EBV-carrying lymphoblastoid and Burkitt's lymphoma cell lines. There is no obvious correlation between the size of this polypeptide and any properties of the cells from which it is derived, other than those related to the strain of transforming virus. We confirm that the polymorphic region of this molecule is the glycine-alanine copolymer encoded by the third internal repeat of the EBV genome (IR3) and we consider the significance of this domain.
Immobilization of Raji cells on surface coated with anti-lymphocyte globulin (ALG) at low cell densities lead to the synthesis of Epstein-Barr virus (EBV) early antigen (EA) in up to 5% of the cells. At higher cell densities the percentage of antigen-positive cells decreased and at confluency no antigen synthesis was observed. Addition of iododeoxyuridine (IdUrd) to low density cultures increased the expression of EA to 20%, whereas in confluent cultures the cells could not be induced to synthesize EA. Treatment of cells in suspension with ALG failed to induced EA synthesis and did not potentiate the effect of IdUrd. Immobilized Raji cells proved to be suitable targets for superinfection with EBV derived from P3HR1 cultures. ...
This study will examine the effects of long-term antiviral therapy with valaciclovir (Valtrex) on Epstein-Barr virus infection. This virus infects more
An effective method for the synthesis of 7,8-epoxy-1,3,11-cembratriene-15R(α), 16-diol and its in vitro Epstein-Barr Virus Early Antigen (EBV-EA) Activation Chemopreventive Assay are reported. This semisynthetic product is a new cembranoid with a potent tumor inhibitory activity that is expected to be a lead compound for a new class of chemopreventive agents of marine origin.
ID:1,Note:Change of Day(s) Test Setup to every Monday, Wednesday and Friday w.e.f 1 August 2014. ,Date:2014-07-29T08:45:00.000Z,Deleted:false,IsNew:true ...
The numerous cases which were treated showed a positive clinical response in greater or lesser degree. The earliest cases were studied intensively and were readied for publication.. The tumors that responded to a greater degree to our treatment, as was expected, are the ones with the greatest degree of undifferentiation; because of their antigenic characteristics and because of the greater degree of dissimilarity between them and the genotypes and antigenic characteristics of normal cells, they have more probability of eliciting an efficient immunologic response.. Histological studies which were made give us certain possible mechanisms of action which correlate with the clinical response obtained.. Microscopic studies showed the almost complete delimitation of the tumoral areas by connective-vascular structures which seem to have diverse morphologies according to their proximity to the tumor and the level of their chronological development, having at first a thick endothelial wall with ...
We assessed the value of the cytomegalovirus (CMV) antigenemia assay for diagnosing primary CMV infection in adults. The CMV antigenemia assay was performed for 40 patients admitted to our unit over a 2-year period with unexplained fever and suspected primary CMV infection. Nine of the 10 patients with primary CMV infection had positive CMV antigenemia assays, and the results were available within 5 hours. All 10 patients had a mononucleosis-like syndrome. All but one of the 30 other patients had negative CMV antigenemia assays. A false-positive result was obtained for a patient with systemic lupus erythematosus. Overall, the CMV antigenemia assay was 90% sensitive and 96% specific for the diagnosis of primary CMV infection. Therefore, the CMV antigenemia assay appears to be a simple, rapid, inexpensive test for the diagnosis of primary CMV infection in hospitalized adults.. ...
The Epstein-Barr Virus Capsid Antigen (VCA) IgM ELISA Kit is intended for the measurement of IgM antibodies to Epstein-Barr Virus Capsid Antigen (VCA) in a sample. This kit utilizes Epstein-Barr VCA antigen (P3H3 cell extract, cultured in human Burkitt lymphoma cells).
TY - JOUR. T1 - Synthetic peptides deduced from the amino acid sequence of Epstein‐Barr virus nuclear antigen 6 (EBNA 6). T2 - Antigenic properties, production of monoreactive reagents, and analysis of antibody responses in man. AU - Falk, K.. AU - Linde, A.. AU - Johnson, D.. AU - Lennette, E.. AU - Ernberg, I.. AU - Lundkvist, A.. PY - 1995/8. Y1 - 1995/8. N2 - Studies on the antibody responses to various Epstein‐Barr virus (EBV) antigens have been instrumental in the understanding of the seroepidemiology and diagnosis of this viral infection and the subsequent carrier state. While antibodies to the viral capsid antigen (VCA), early antigen (EA), and nuclear antigens 1 and 2 (EBNA 1 and 2) have been well characterized, the antibody response to the other nuclear antigens is not well understood. EBNA 6 is expressed by lymphoblasts during acute EBV infection and may be an important antigen for diagnosis and evaluation of the immune response. In order to analyze the antibody response to EBNA ...
Epithelial-mesenchymal transition is an important mechanism in cancer invasiveness and metastasis. We had previously reported that cancer cells expressing Epstein-Barr virus (EBV) latent viral antigens EBV nuclear antigen EBNA3C and/ or EBNA1 showed higher motility and migration potential and had a propensity for increased metastases when tested in nude mice model. We now show that both EBNA3C and EBNA1 can modulate cellular pathways critical for epithelial to mesenchymal transition of cancer cells. Our data confirms that presence of EBNA3C or EBNA1 result in upregulation of transcriptional repressor Slug and Snail, upregulation of intermediate filament of mesenchymal origin vimentin, upregulation of transcription factor TCF8/ZEB1, downregulation as well as disruption of tight junction zona occludens protein ZO-1, downregulation of cell adhesion molecule E-cadherin, and nuclear translocation of β-catenin. We further show that the primary tumors as well as metastasized lesions derived from EBV ...
The various antigen complexes of the Epstein-Barr virus (EBV) are broadly classified as the viral capsid antigen (VCA), diffuse early antigen (EA-D), restricted early antigen (EA-R), membrane antigen (MA) and the Epstein-Barr nuclear antigen (EBNA). The different EBV-related diseases may be differentiated according to the reactivity of these different classes of antibodies towards the various classes of antigen complexes. However, with the recent development of molecular biology, it is now known that the individual polypeptides of the different EBV antigen complexes can be used as serological markers for the detection of nasopharyngeal carcinoma (NPC). Among the useful serological markers which have been used in enzyme-linked immunosorbent assay (ELISA) for the detection of NPC are the gp125 from the VCA complex (IgA), pp58 from the EA-D complex (IgG), ribonucleotide reductase (IgG and IgA), DNase (IgA) and thymidine kinase (IgA) from the EA-R complex, gp 250/200 from the MA complex (IgA) and ...
In human recurrent herpetic lesions epidermal keratinocytes are induced to express HLA class II (DR) antigens. Keratinocytes derived from human split skin and cultured in vitro were induced to express HLA-DR but not -DQ antigens with IFN gamma preparations. These stimulated keratinocytes presented herpes simplex antigen directly to autologous blood-derived T lymphocytes in four of four subjects (stimulation indices: 1.5-2.7), suggesting that keratinocytes may have an accessory herpes simplex virus (HSV) antigen-presenting role in addition to the Langerhans cells and macrophages in herpetic skin lesions. Blood mononuclear cells from eight herpes simplex seropositive subjects which were activated in vitro by HSV antigen for 6 d showed cytotoxicity specific for HSV in infected autologous keratinocytes. This was significantly increased by prestimulation with IFN gamma (51-56% to 83-85%). In four of eight patients some cytotoxicity also occurred against uninfected, IFN gamma-stimulated keratinocytes. ...
Epstein-Barr Nuclear Antigen 1 (EBNA1) is a multifunctional protein encoded by EBV. EBNA1s role in maintaining EBV in latently proliferating cells, by mediating EBV genome synthesis and nonrandom partitioning to daughter cells, as well as regulating viral gene transcription, is well characterized. Less understood are the roles of EBNA1 in affecting the host cell to provide selective advantages to those cells that harbor EBV. In this review we will focus on the interactions between EBNA1 and the host cell that may provide EBV-infected cells selective advantages beyond the maintenance of EBV.
Blog on CMV antigen product: The CMV n/a (Catalog #MBS318657) is an Antigen produced from HF Cells and is intended for research purpos...
5GTC: Crystal structure of complex between DMAP-SH conjugated with a Kaposis sarcoma herpesvirus LANA peptide (5-15) and nucleosome core particle
After washing to remove non-specifically bound antibodies, the substrate is incubated with an anti-human antibody conjugated to fluorescein. When results are positive, a stable three-part complex forms, consisting of fluorescent antibody bound to human antinuclear antibody that is bound to nuclear antigen. This complex can be visualized with the aid of a fluorescent microscope. In positive samples, the cell nuclei will show a bright apple-green fluorescence with a staining pattern characteristic of the particular nuclear antigen distribution within the cells. If the sample is negative for ANA, the nucleus will show no clearly discernible pattern of nuclear fluorescence. The cytoplasm may demonstrate weak staining while the non-chromosome region of mitotic cells demonstrates brighter staining ...
Proceeding/Conference:Biennial Conference of the International Association for Research on Epstein-Barr Virus and Associated Diseases ...
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This topic contains 47 study abstracts on Epstein-Barr Virus Infections indicating that the following substances may be helpful: Curcumin, Licorice, and Turmeric
Comparison of washed nasopharyngeal cells and whole nasal secretions for detection of respiratory syncytial virus antigens by enzyme-linked immunosorbent assay ...
Bovine Serum Albumin (BSA) Antigen Capture ELISA - Photometric, 5 x 96 Tests. |p|The advent of biological products produced using animal components has created a need to ensure their removal prior to use.
When the "mono spot" test is negative, the optimal combination of EBV serologic testing consists of the antibody titration of four markers: IgM and IgG to the viral capsid antigen, IgM to the early antigen, and antibody to EBNA ...
View Notes - Immunogenes or Antigens from STEP 1 at Montgomery College. ‫بسم اللة الرحمن‬ ‫الرحيم‬ Immunogens Or Antigens Immunogens Or Antigens Immunogens or
There are no specific protocols for Recombinant Cytomegalovirus pp28 protein (ab43038). Please download our general protocols booklet
Formalin-inactivated whole murine mammary tumor virus (MuMTV), MuMTV membranes, the acid-soluble component of MuMTV, and purified MuMTV glycoprotein with a molecular weight of 55,000 (gp55; also designated as gp52) were used as vaccines in an attempt to identify the MuMTV antigen(s) that can protect mice from exogenous MuMTV infection and subsequent tumor development. Formalin-inactivated whole MuMTV, MuMTV membranes, and purified MuMTV gp55 were effective immunogens, whereas the acid-soluble component of MuMTV (which consists mainly of MuMTV gp55) failed to protect mice from challenge with live virus. These results suggest that (a) MuMTV gp55 is the major immunizing antigen and (b) its native conformation must be maintained for it to be an effective vaccine.. ...
Looking for the definition of h-2 antigens? Find out what is the full meaning of h-2 antigens on Abbreviations.com! Viral Capsid Antigens is one option -- get in to view more @ The Webs largest and most authoritative acronyms and abbreviations resource.
The basic components of the diagnostic test systems are antigens and specific antibodies. The main objective of developing express tests for the diagnosis of bovine leukemia virus (BLV) is to obtain a virus antigen drug, which is very time-consuming to prepare. This problem can be solved by producing anti-idiotype antibodies that have a chemical structure identical to that of the viral antigen and does not require large expenditures to manufacture [1, 2 ...
Free resource for searching and exporting immune epitopes. Includes more than 95% of all published infectious disease, allergy, autoimmune, and transplant epitope data.
Bandlow, G. und Koszinowski, Ulrich H. (1974): Increased cellular immunity against host cell antigens induced by vaccinia virus. In: Archiv für die gesamte Virusforschung, Vol. 45: S. 122-127 ...
... Antigen processing is a biological process that prepares antigens for presentation to special cells of the immune system called T lymphocytes.
Discussion. In our study, we found 2 age peaks in the incidence of primary infection by EBV before adolescence: 2-4 years and 7 years. Historically, the literature has described a higher incidence in adolescence.2 In our sample, up to 64% of patients with a serologic diagnosis of primary infection by EBV were aged less than 10 years, probably because older children have a more florid presentation, which in many cases leads to a clinical diagnosis without the ordering of confirmatory laboratory tests that was the basis of our study.14-16. Despite the higher frequency in younger children, our study found a predominance of the typical presentation (65 cases). In the group of patients with typical features, 13 only had 2 symptoms and 52 more than 2 symptoms. However, in many instances the third symptom belonged to the composite variables "other symptoms", which encompassed clinical manifestations that are not specifically part of mononucleosis syndromes. This approach to the classification of ...
We have two kinds of cells in our body which are used to fight diseases: B cells and T cells. Whenever an antigen attacks our body for the first time, these cells create a code to fight those antigens. Once the code is created, next time the antigen attacks, these cells are able to fight the antigen very fast as they already have the code created for that specific antigen ...
Congenital human cytomegalovirus (HCMV) infection is the major cause of birth defects and a precise definition of the HCMV-specific T-cell response in primary ...
Epstein-Barr virus (EBV) affects more than 90 percent of the population worldwide and was the first human virus found to be associated with cancer.
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Epstein-Barr virus (EBV) is a gammaherpesvirus that causes infectious mononucleosis and is associated with several human malignancies. In vitro, EBV induces the activation and continuous proliferation of primary human B cells, which carry the viral genome as a latent episome. One of the latency-associated proteins is the EBV nuclear antigen leader protein (EBNA-LP). EBNA-LP is strongly expressed at the initiation of the transformation process and has been previously reported to assist the activation of genes by EBNA-2. To investigate the role and mechanism of action of EBNA-LP in the context of viral infection and B cell transformation, I have generated EBNA-LP knockout EBVs (LP-KO i) and their revertants (LP-REV i) by recombineering in the B95.8 bacterial artificial chromosome (BAC). We found that B cells infected with LP-KO i were able to undergo limited proliferation. However, we were not able to establish LCLs after infection of EBV-negative B cells. We also found that LP-REV i was somewhat ...
Secondary effector T-cell populations generated by cross-priming with heterologous influenza A viruses operate only in H-2K or H-2D compatible situations, when assayed on SV40-transformed target cells infected with a range of influenza A viruses. The H2-Kb allele is associated with a total failure in the generation of influenza-immune cytotoxic T cells, though this is not seen for the primary response to vaccinia virus. In both influenza and vaccinia development of effector T cells operating at H-2Db is greatly depressed in B10.A(2R) (kkkddb) and B10.A(4R) (kkbbbb), but not in B10 (bbbbbb), mice. However, there is no defect in viral antigen expression at either H-2Kk or H-2Db in B10.A(2R) target cells. This apparently reflects some inadequacy in the stimulator environment, as (A/J X B6) F1 T cells can be induced to respond at H-2Db when exposed to vaccinia virus in an irradiated B6 but not in a B10.A(4R) recipient. The present report, together with the accompanying paper by Zinkernagel and ...
Nasopharyngeal carcinoma (NPC) is an epithelial malignancy, which commonly occurs in Southern China, Taiwan, North Africa and Southeast Asia. Nasopharyngeal carcinoma is strongly associated with Epstein-Barr virus infection. The p53 tumour suppressor protein is rarely mutated in NPC suggesting that the inactivation of p53 pathway in NPC could be due to the presence of EBV proteins. The aim of this work was to determine the effects of EBV proteins namely LMP1 and LMP2A on the expression levels of p53 protein. In this work we found that LMP1, but not LMP2A, decreased p53 protein levels. Overexpression of LMP1 resulted in increased ubiquitination of p53 suggesting that the decreased p53 protein levels by LMP1 was due to increased degradation of the protein. The reduction of p53 protein levels was independent of the PI3K-Akt pathway. LMP1, but not LMP2A, reduced p53 protein levels through the increase in the polyubiquitination of p53 protein and was independent of the PI3K-Akt pathway.
Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. According to the considered strain, VP4 seems to essentially target sialic acid and/or the integrin heterodimer ITGA2/ITGB1 (By similarity).
Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. According to the considered strain, VP4 seems to essentially target sialic acid and/or the integrin heterodimer ITGA2/ITGB1 (By similarity).
The radioimmunoprecipitation assay for the murine mammary tumor virus (MuMTV) was used to detect naturally occurring antibodies against MuMTV in 3 groups of highly inbred mouse strains. 1) Some strains had high incidences of mammary tumors, such as strains GR and C3H. Antibodies against MuMTV were detected in the sera of females of these strains at early ages. 2) Some mouse strains had low incidences of mammary tumors with an intermediate MuMTV expression, such as strains C3Hf, RIIIf, and BALB/c. Some females of these strains developed antibodies against MuMTV. Hormone treatment of these mice resulted in an increase in the proportion of mice carrying antibodies against MuMTV. 3) Some mouse strains were MuMTV-free, such as strains O20, C57BL, and Gr-Mtv2-. No antibodies against MuMTV were detected in the sera of these mice. However, antibodies against MuMTV appeared in the sera of these animals after hormone treatment. The presence of a natural humoral immunity toward MuMTV appeared to be related ...
Viruses that naturally infect cells expressing both MHC I and MHC II molecules render themselves potentially visible to both CD8+ and CD4+ T cells through the de novo expression of viral antigens. Here we use one such pathogen, the B-lymphotropic Epstein-Barr virus (EBV), to examine the kinetics of these processes in the virally-infected cell, comparing newly synthesised polypeptides versus the mature protein pool as viral antigen sources for MHC I- and MHC II-restricted presentation. EBV-transformed B cell lines were established in which the expression of two cognate EBV antigens, EBNA1 and EBNA3B, could be induced and then completely suppressed by doxycycline-regulation. These cells were used as targets for CD8+ and CD4+ T cell clones to a range of EBNA1 and EBNA3B epitopes. For both antigens, when synthesis was induced, CD8 epitope display rose quickly to near maximum within 24 h, well before steady state levels of mature protein had been reached, whereas CD4 epitope presentation was delayed ...
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Cross-priming is a critical component of T cell responses to cancers and viruses, and involves transfer of antigen from antigen donor cells to the antigen presenting cells. In spite of the centrality of antigen in this process, the influence of the quantity of antigen expressed by the antigen donor cell on the efficiency of cross-priming remains unexamined. Here, I describe the creation of a novel system where the model antigen ovalbumin is expressed in P815 (d haplotype) cells under the control of an inducible promoter, producing a large amount of antigen synthesis upon induction. However, even in the un-induced condition, a very low level of ovalbumin can be detected using sensitive methods to amplify the weak signal. I have used titrated quantities of uninduced and induced cells, expressing vastly different quantities of ovalbumin, and have monitored cross-priming of the endogenous anti-OVA CD8+ T cell response quantitatively in C57BL/6 mice (b haplotype), using in vivo cytolytic T lymphocyte assays.
Mouse anti Epstein-Barr virus viral capsid antibody, clone 0231 recognizes the viral capsid antigen of Epstein-Barr Virus (EBV), also know
Define Proliferating cell nuclear antigen. Proliferating cell nuclear antigen synonyms, Proliferating cell nuclear antigen pronunciation, Proliferating cell nuclear antigen translation, English dictionary definition of Proliferating cell nuclear antigen. also an·ti·gene n. A molecule that is capable of binding to an antibody or to an antigen receptor on a T cell, especially one that induces an immune...
Precipitin antigen was prepared from cultures infected with V-Z virus. Five separate antigens were identified. Reactions with radiolabeled antigen were detected by autoradiography. Partial purification of antigens could be accomplished in a discontinuous sucrose gradient, yet too little antigen was recovered from DEAE to make this a practicable purification method. Most of the antigenic activity was eluted from G-200 Sephadex. Antigens were identified by their degree of chemical stability. The α antigen was the only one which was stable at pH 3. The β antigen was destroyed by CsCl or DOC. DOC released the γ Δ, and ε antigens from the infectious viral particle.. ...
Latency-associated nuclear antigen (LANA) is central to episomal tethering, replication and transcriptional regulation of 2-herpesviruses. LANA binds cooperatively to the terminal repeat (TR) region of the viral episome via adjacent LANA binding sites (LBS), but the molecular mechanism by which LANA assembles on the TR remains elusive. We show that KSHV LANA and MHV-68 LANA proteins bind LBS DNA using strikingly different modes. Solution structure of LANA complexes revealed that while kLANA tetramer is intrinsically bent both in the free and bound state to LBS1-2 DNA, mLANA oligomers instead adopt a rigid linear conformation. In addition, we report a novel non-ring kLANA structure that displays more flexibility at its assembly interface than previously demonstrated. We identified a hydrophobic pivot point located at the dimer-dimer assembly interface, which gives rotational freedom for kLANA to adopt variable conformations to accommodate both LBS1-2 and LBS2-1-3 DNA. Alterations in the ...
EBV is a member of the gamma-herpesvirus family. It is an enveloped virus of about 150-180 nm diameter with an icosahedral nucleocapsid containing a double-stranded DNA viral genome of about 172 000 base pairs. The nucleocapsid is composed of a major 160 kDa nonglycosylated polypeptide and a minor 125 kDa glycoprotein, both of which form part of the viral capsid antigen (VCA) complex. The virus lipoprotein envelope contains at least three virally-encoded glycoproteins, designated the membrane antigen (MA) complex. Two antigenically-related MA glycoproteins, gp340 and gp220, potentiate binding of the virus to a specific cell surface receptor molecule during the infection of target cells. The third MA glycoprotein, gp85, is involved in the fusion of bound virus with the host cell membrane. Following fusion, viral DNA is released into the cell and is transcribed and replicated in the nucleus, where it persists as multiple episomal copies.. The 140 kDa C3d complement receptor molecule (CD21) is the ...
Researchers at the University of Pennsylvania School of Medicine have shed new light on how Kaposis Sarcoma-associated Herpes Virus (KSHV) subverts normal cell machinery to cause cancer. A KSHV protein called latency-associated nuclear antigen, LANA for short, helps the virus hide out from the immune system in infected cells. When LANA takes the place of other proteins that control cell growth, it can cause uncontrolled cell replication.
Kaposis sarcoma associated herpesvirus (KSHV) or human herpesvirus 8 (HHV8) is a major etiological agent for multiple severe malignancies in immune-compromised patients. KSHV establishes lifetime persistence in the infected individuals and displays two distinct life cycles, generally a prolonged passive latent, and a short productive or lytic cycle. During latent phase, the viral episome is tethered to the host chromosome and replicates once during every cell division. Latency-associated nuclear antigen (LANA) is a predominant multifunctional nuclear protein expressed during latency, which plays a central role in episome tethering, replication and perpetual segregation of the episomes during cell division ...
Infectious mononucleosis is a very widespread viral disease caused by the Epstein-Barr virus. The disease is also known as EBV Infectious Mononucleosis or Pfeiffers Disease or Filatovs Disease and colloquially as kissing disease, mono (North America) and as glandular fever in other English-speaking countries. The disease is characterized by fever, sore throat and fatigue.
Rabies is a zoonotic viral disease caused by a virus of the genus Lyssavirus that affects several species of mammals. Rabies remains a global public health threat that kills more than 55,000 people per year mainly in ...
Both react strongly with HCV positive sera; the choice of antigen may be a function of the particular assay format chosen, however, more commonly, it appears that the fusion protein was the antigen of choice. The protein is soluble in aqueous buffer and may be admixed with the HCV capsid antigen prior to coating on to microplate wells. The protein comes as a 500 ug/ml (again setting 1 mg/ml equivalent to an OD (280nm) in Tris buffer, of 1.0). Individual Elisa protocols can be developed, such as Capsid antigen alone (Example 1); NS3 antigen alone (Example 2); or, A combination of antigens (Example 3). Using a Capsid: NS3 ratio of 4:1 in the coating mixture appears to provide the most favorable signal-to-noise ratios in the subsequent assay: Access to the individual antigen component thus enables the determination of precise parameters for an optimized assay.. To view an SDS PAAGE image of this antigen, please click HERE. To review the seroconversion data (using a standard seroconversion panel ...
Looking for nuclear antigen? Find out information about nuclear antigen. see immunity immunity, ability of an organism to resist disease by identifying and destroying foreign substances or organisms. Although all animals have... Explanation of nuclear antigen
Did you know, you probably have virus lurking in the shadows? Its called the Epstein-Barr virus (EBV) and an estimated 90 percent of us have it. Fortunately, for most of us it lies dormant, like a sleeping giant. Only when something triggers a reactivation of EBV does this virus rear its ugly head.
After you stop the AntigenService service, Antigen Scheduled Tasks remain on the server. This is particularly poignant on a cluster server. When Antigen Scheduled Tasks run on a passive node, they start up Antigen services, which try to connect to Exchange on the local node and access Antigen database files on the shared drive. The Antigen services are unable to achieve any of this and there is no immediate danger to the live nodes activities, but they will cause various errors in the Windows Application event log ...
Epstein-Barr virus (EBV) is an extremely successful pathogen, being able to establish a lifelong latent infection within B lymphocytes of its human host, with o...
Enzyme immunoassay for the detection of IgM antibodies to the early antigen of Epstein-Barr Virus in human serum or plasma. SmartEIA kit is specifically designed for automated analysis using the Agility instrument.
Initial results from clinical trials show new drug therapies are effective against lymphomas and other cancers connected to the Epstein-Barr virus.
You are at: Home » Products » Bio-Technology Products » Rotavirus Group A Antigen Test Stick and Device In total 82043 number ofProductsinfo,Released today. 6855 number of ...
In experiments measuring the length of time an antigen stays stuck, DM makes sure an infected cell holds onto a microbe long enough to catch the attention of immune cells in the first place, Sadegh-Nasseri says.. To uncover DMs expanded job, Sadegh-Nasseri isolated a protein antigen from the flu virus as a test case and found that cells with DM normally hold on for about six days, long enough for symptoms like sniffles and fever, as signs of immune battle, to develop. When they removed DM from normal cells, the cells did not bind the flu antigen at all. Later, when they mutated the antigen-binding part of the cell, the flu antigen "fell off" after only 10 minutes.. When the scientists studied the 3-D shape of the part of the cell that tries on the antigen, they discovered that the antigen fell off after 10 minutes whether DM was there or not, but only when one specific chemical bond was disrupted.. "DM somehow alters this chemical bond to make antigens fall off a thousand times faster than ...
Australský antigen je označení pro antigen viru hepatitidy B, který se nachází na povrchu virových částic. Označuje se též jako „surface antigen" (HBsAg). Diagnostika přítomnosti antigenu v krvi je důležitá pro diagnostiku hepatitidy B ...
A subgroup" antigens are inherited, as are other ABO antigens with A1 being dominant over A2. Individuals who are phenotypically A1 may be genotypically A1O, A1A1, or A1A2. A phenotypically A2 individual may be genotypically A2A3. These alleles are passed to offspring in the same manner as other ABO antigens ...
Information on this virus which causes a number of diseases, including infectious mononucleosis, the symptoms, complications, diagnosis and treatment. ...
Antigen An atigen is any substance that cause your immune system to produce antibodies against it. The antigen may be a foreign substance from the environment Such as chemicals bacteria viruses, or pollen) or formed within the body
Looking for the meaning of ca-19-9 antigen? Find out what is the meaning of ca-19-9 antigen on Phrases.net! The Webs largest and most authoritative phrases and idioms resource.
I need some HLA-I ANTIGEN ,who can tell me where have the antigen= in commercially or who can give me some ,thanks. my E-mail: sdwcy at yesky.com =D6=C2 =C0=F1=A3=A1 sdwcy sdwcy at yesky.com ...
The Development of a Radioimmuno-Assay for Carcino-Embryonic Antigen with some Applications. Clinical Evaluation of Cercino-Ernbryonic Antigen, ...
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Antigen for Exchange found Happy99.exe infected with W32/Ska.A.Worm virus. The file is currently Deleted. The message, Newbie Question: Generating Timer Events wit Tkinter, was sent from Jim Bishop and was discovered in Gabe Newcomb\Inbox located at Captura Software, Inc./CAPTURA/GIBRALTAR ...
Pleiotrophic Functions of Epstein-Barr Virus Nuclear Antigen-1 (EBNA-1) and oriP Differentially Contribute to the Efficacy of Transfection/expression of Exogenous Gene in Mammalian Cells ...
Intracellular reovirus antigen localizes in the area occupied by the achromatic figure of mitotic cells. In untreated interphase cells, viral antigen forms a filamentous, intracytoplasmic reticulum, but in the presence of certain spindle poisons it consolidates into clumps. The specificity of this latter phenomenon was investigated in this study. The results obtained are as follows.. ...
Herpes? Thats not something that many people want to think about. But 1 in 4 people DO think about it, because they have it. As a matter of fact, 70 million are afflicted with STDs in the U.S. alone and an estimated over 400 million worldwide. Are you one of them? I have Herpes too. I have find my herpes mate on http://www.STDromance.com . ...
Mononucleosis often referred to as mono, the kissing disease, or glandular fever, is a viral infection commonly caused by the Epstein-Barr virus. Although, it
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I am nearing my preparation for my next LDA (low dose antigen) therapy. I start the protocol in three days. I have mentioned this therapy in past posts and will attempt to explain it this weekend when I start.
Scientists show that cytomegalovirus vectors can be genetically programmed to prod CD8+ cells into recognizing a broader range of SIV viral epitopes than they usually notice.
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LeukinFeron, 100000 U. |p class=db_paraghraph|Human LeukinFeron is a combines preparation of natural cytokines involved in cellular reactions elimination of pathogen (antigen).
The Epstein-Barr virus nuclear antigen 2 (EBNA-2) is one of the six EBV viral nuclear proteins expressed in latently infected B lymphocytes is a transactivator protein. EBNA2 is involved in the regulation of latent viral transcription and contributes to the immortalization of EBV infected cells. EBNA2 acts as an adapter molecule that binds to cellular sequence-specific DNA-binding proteins, JK recombination signal-binding protein (RBP-JK), and PU.1 as well as working with multiple members of the RNA polymerase II transcription complex. EBNA2 requires C-promoter binding factor 1 (CBF1) to aid in binding to its cis-responsive DNA element, the C promoter (Cp). Binding occurs during infection, to generate a 120kb transcript that encodes all nuclear antigens required for immortalization by EBV.2 Mutation of EBNA2 amino acid 323 and 324, which are located within a highly conserved amino acid motif, abolished the interaction with CBF1.3 This same mutation also abolished the ability of EBNA-2 to ...
Immune adherence hemagglutination (IAHA) was compared to complement fixation (CF), using standard procedures, for serological testing of human sera with a number of commercially available antigens. The antigens included herpes simplex, measles, cytomegalo-, and influenza (type B) viruses, as well as Mycoplasma pneumoniae and Chlamydia psittaci (Chlamydia group). The IAHA test was found to be as specific as the CF test, but 4 to 20 times as sensitive with all antigens tested. Antigen titers were also higher with the IAHA method, and the time required to complete the test was only 4 h for the IAHA method, compared with 20 h for the CF method. The increased sensitivity of the IAHA test should permit its use for determination of immunity, as well as for serodiagnosis of recent infections.
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In raising murine hybridoma antibodies against Epstein-Barr virus (EBV)-induced membrane antigens (MA), we found one antibody that blocked the release of infectious EBV from cultured P3HR-1 cells. This monoclonal antibody (mAb) recognized a 200 kD, phosphonoacetic acid-sensitive (late) MA, and did not directly neutralize virus without complement. When this mAb was added to 33 degrees C-cultured, spontaneously EBV-producing P3HR-1 cells, the intracellular expression of viral capsid antigen and infectious virus was not inhibited, but the appearance of infectious virus in the culture medium was significantly reduced. The duration of this suppression was dependent upon the concentration of the mAb, an effect being observed to a 1:4 X 10(5) titer of the ascites mAb preparation. A more acute effect of suppression of EBV release was observed in a second model of 12-o-tetradecanoyl phorbol-13-acetate and n-butyrate induction of EBV in 37 degrees C-cultured P3HR-1 cells. Again, intracellular infectious ...
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There are many outside agents that could become antigens. Among the agents that are potentially antigenic are egg whites, pollen, transplanted tissue proteins and plenty of other agents that could cause a reaction of the immune system in order to take care of the invasion.. These outside antigens are also known as non-microbal or non-self antigens. One outside source of imunogenic antigens are vaccines. They are often given to people in order to prepare themselves for a potential illness.. Outside antigens are known as exogenous antigens. The common way that these antigens enter the body is through inhalation, injection, or ingestion. Often times, the immune system reacts to the antigens in a less than clinical fashion.. The process of the antibodies taking on the antigens are either endocytosis or phagocytosis. These antigens are brought to the cells that present antigens. The antigens are then broken down into pieces so that they can be processed.. The pieces are taken to the T helper cells so ...
0052] One class of antigens as comprised in the herein defined inventive vaccine/inhibitor combination comprises tumour antigens. "Tumour antigens" are preferably located on the surface of the (tumour) cell. Tumour antigens may also be selected from proteins, which are overexpressed in tumour cells compared to a normal cell. Furthermore, tumour antigens also include antigens expressed in cells which are (were) not themselves (or originally not themselves) degenerated but are associated with the supposed tumour. Antigens which are connected with tumour-supplying vessels or (re)formation thereof, in particular those antigens which are associated with neovascularization, e.g. growth factors, such as VEGF, bFGF etc., are also included herein. Antigens connected with a tumour furthermore include antigens from cells or tissues, typically embedding the tumour. Further, some substances (usually proteins or peptides) are expressed in patients suffering (knowingly or notknowingly) from a cancer disease ...
Title:Chemoprevention of Skin Cancer: Effect of Lawsonia inermis L. (Henna) Leaf Powder and its Pigment Artifact, Lawsone in the Epstein- Barr Virus Early Antigen Activation Assay and in Two-Stage Mouse Skin Carcinogenesis Models. VOLUME: 13 ISSUE: 10. Author(s):Govind J. Kapadia, G. Subba Rao, Rajagopalan Sridhar, Eiichiro Ichiishi, Midori Takasaki, Nobutaka Suzuki, Takao Konoshima, Akira Iida and Harukuni Tokuda. Affiliation:College of Pharmacy, Howard University, 2300 4th Street, NW, Washington, DC 20059, USA.. Keywords:Chemoprevention, epstein-Barr virus antigen assay, henna leaf powder, lawsone, mouse skin cancer models.. Abstract:In continuation of our studies with chemoprevention potential of plant-derived naphthoquinone derivatives, leaf powder of the medicinal plant Lawsonia inermis L, commonly known as henna, was evaluated by its inhibition of the Epstein-Barr virus early antigen (EBV-EA) activation induced by the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) in Raji ...
TY - JOUR. T1 - The secreted g protein of human respiratory syncytial virus antagonizes antibody-mediated restriction of replication involving macrophages and complement. AU - Bukreyev, Alexander. AU - Yang, Lijuan. AU - Collins, Peter L.. PY - 2012/10/1. Y1 - 2012/10/1. N2 - The respiratory syncytial virus (RSV) G and F glycoproteins are the neutralization antigens, and G also is expressed in a soluble form (sG). Previously, sG was demonstrated to reduce the efficiency of RSV antibody-mediated neutralization by serving as an antigen decoy and to inhibit the antibody-mediated antiviral effects of Fc receptor-bearing leukocytes. The present study demonstrated that effective antibody-mediated restriction in vivo, and the evasion of this restriction by sG, involves pulmonary macrophages and complement, but not neutrophils.. AB - The respiratory syncytial virus (RSV) G and F glycoproteins are the neutralization antigens, and G also is expressed in a soluble form (sG). Previously, sG was demonstrated ...
TY - JOUR. T1 - Development of specific nucleic acid probes for the differentiation of porcine rotavirus serotypes. AU - Johnson, Michael E.. AU - Paul, Prem S.. AU - Gorziglia, Mario. AU - Rosenbusch, Ricardo. PY - 1990/9. Y1 - 1990/9. N2 - A dot blot hybridization assay is described for the detection and differentiation of porcine rotavirus serotypes. Recombinant complementary DNA (cDNA) representing gene 9 (the gene encoding the neutralization antigens in VP7 glycoprotein) from OSU (porcine rotavirus serotype 1) and Gottfried (porcine rotavirus serotype 2) strains were used to determine the optimal hybridization conditions which allow specific detection of group A porcine rotaviruses. Probes were prepared by excision of the inserts from the recombinant plasmids and radiolabeling of cDNA with 32P by the random primer extension method. Probes were hybridized at various stringencies with viral RNA from different rotavirus serotypes bound to nylon membranes. Hybridization at low stringency (26% ...

Viral AntigensViral Antigens

An easy-to-use directory for life science and biomedical research products. Find special deals on products, order catalogs and browse product lines from suppliers of reagents, antibodies, laboratory equipment, and more.
more infohttp://www.biosupplynet.com/cfdocs/products/prod_supp.cfm?prod_id=2328

Viral Antigens | ProSpecViral Antigens | ProSpec

A viral antigen is a protein encoded by the viral genome.A viral protein is an antigen specified by the viral genome that can ... A viral Antigen is an antigen with multiple antigenicities that is protein in nature, strain-specific, and closely associated ... A viral antigen is a protein encoded by the viral genome.A viral protein is an antigen specified by the viral genome that can ... About Viral Antigens:. A viral Antigen is an antigen with multiple antigenicities that is protein in nature, strain-specific, ...
more infohttps://www.prospecbio.com/viral_antigens

Modified Viral Antigens | GreenMedInfo | Keyword | Natural MedicineModified Viral Antigens | GreenMedInfo | Keyword | Natural Medicine

Additional Keywords : Adverse Reaction, Chromosome, Genomic Variation, Modified Viral Antigens. Anti Therapeutic Actions : ... 1 Abstracts with Modified Viral Antigens Research. Filter by Study Type. Human: Case Report. ... 1 Problematic Actions Researched for Modified Viral Antigens Name. AC. CK. Focus. ...
more infohttp://www.greenmedinfo.com/keyword/modified-viral-antigens

Viral antigens elicit augmented immune responses in primary Sjögrens syndrome.  - PubMed - NCBIViral antigens elicit augmented immune responses in primary Sjögren's syndrome. - PubMed - NCBI

Viral antigens elicit augmented immune responses in primary Sjögrens syndrome.. Björk A1, Thorlacius GE1, Mofors J1, ... We observed augmented innate and adaptive immune responses in pSS following viral antigen exposure suggesting an underlying ... Systematic studies of immune responses to microbial antigens in vivo may be performed during vaccination. In the present study ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/31665501

ASMscience | Viral Antigen DetectionASMscience | Viral Antigen Detection

Diagnosis of viral infections via viral antigen detection methods such as immunofluorescence (FA), immunochromatography ( ... This chapter deals with principles of FA, IC, and EIA and their contemporary applications in viral antigen detection. ... offer many of these attractive features and are useful for direct detection of viral antigens in an array of clinical specimens ... Whether the detection method is FA, rapid IC, or EIA, detection of antigens of the common respiratory viruses (i.e., adenovirus ...
more infohttp://www.asmscience.org/content/book/10.1128/9781555819156.ch08

Chikungunya E1 Viral Antigen Recombinant | ProSpecChikungunya E1 Viral Antigen Recombinant | ProSpec

Polyprotein is processed by host signalases, resulting in E3, E2 & E1 forming viral hetero-trimeric spikes. The viral spikes ... The structural polyprotein is translated from a viral sub genomic mRNA, while as the 5 structural proteins (capsid, E3, E2, 6K ... 80 glycoprotein spikes are C- terminally anchored within the viral envelope. ... majorly contains E2 and E1 facilitate cell receptor recognition, cell entry thru pH-dependent endocytosis and support viral ...
more infohttps://www.prospecbio.com/chikungunya_e1

Western and dot immunoblotting analysis of viral antigens and antibodies: application to murine hepatitis virus.  - PubMed -...Western and dot immunoblotting analysis of viral antigens and antibodies: application to murine hepatitis virus. - PubMed -...

Western and dot immunoblotting analysis of viral antigens and antibodies: application to murine hepatitis virus.. Talbot PJ, ... Monoclonal antibodies directed against previously defined epitopes on the viral proteins were used as probes to detect viral ... Viral proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred ... highly sensitive dot immunoblotting assay was used to analyze the sensitivity to denaturation of various epitopes on the viral ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/6208281?dopt=Abstract

Prognostic Significance of Viral Antigens in Liver Tissue | Springer for Research & DevelopmentPrognostic Significance of Viral Antigens in Liver Tissue | Springer for Research & Development

... a number of observations have culminated in the localization of viral antigens in liver tissue by the use of routine histology ... The role of hepatitis B viral antigens and liver membrane antigens as targets. In: Popper H, Schaffner F (eds) Progress in ... Prognostic Significance of Viral Antigens in Liver Tissue. In: Callea F., Zorzi M., Desmet V.J. (eds) Viral Hepatitis. ... Over the past 15 years, a number of observations have culminated in the localization of viral antigens in liver tissue by the ...
more infohttps://rd.springer.com/chapter/10.1007/978-3-642-71350-7_6

EBV Antibody To Viral Capsid Antigen, IgMEBV Antibody To Viral Capsid Antigen, IgM

... ,ARUP Laboratories is a national reference laboratory and a worldwide leader in ... Antigen-Antibody Pens, a set of any 3 pens. 10. Antigen-Antibody Pen For Biotin-tagged antibodies (all species). 11. Antigen- ...
more infohttp://www.bio-medicine.org/medicine-products/EBV-Antibody-To-Viral-Capsid-Antigen--IgM-22586-1/

Pharmaq opens high-tech viral antigen production facility | The Washington Fish Growers AssociationPharmaq opens high-tech viral antigen production facility | The Washington Fish Growers Association

... the Norwegian Medicines Agency granted Pharmaq authorisation to produce viral antigens at their new facility in Kløfta. The ... Pharmaq opens high-tech viral antigen production facility (12/06/17) *Next News Article ... On 1 November 2017, the Norwegian Medicines Agency granted Pharmaq authorisation to produce viral antigens at their new ...
more infohttp://wfga.net/news/pharmaq-opens-high-tech-viral-antigen-production-facility

JCI Insight -
T cells presenting viral antigens or autoantigens induce cytotoxic T cell anergyJCI Insight - T cells presenting viral antigens or autoantigens induce cytotoxic T cell anergy

T cells presenting viral antigens or autoantigens induce cytotoxic T cell anergy. ... T cells presenting viral antigens or autoantigens induce cytotoxic T cell anergy. ... This was a general phenomenon, as T cells presenting influenza (flu) antigen also fail to activate otherwise potent flu- ...
more infohttps://insight.jci.org/articles/view/96173/figure/1

Identification of African Swine Fever Viral Antigens in the Hemolymph of Soft Ticks (Argasidae: Ornithodoros) by the Immunodot...Identification of African Swine Fever Viral Antigens in the Hemolymph of Soft Ticks (Argasidae: Ornithodoros) by the Immunodot...

The immunodot blot test was sensitive and specific for ASFV antigens and has potential field application. Hemolymph from field- ... antigens in the hemolymph from soft ticks (Ornithodoros coriaceus) fed on ASFV-infected pigs. ... Identification of African Swine Fever Viral Antigens in the Hemolymph of Soft Ticks (Argasidae: Ornithodoros) by the Immunodot ... The immunodot blot test was used to identify African swine fever virus (ASFV) antigens in the hemolymph from soft ticks ( ...
more infohttp://www.ajtmh.org/content/journals/10.4269/ajtmh.1986.35.1027

Human B Cells Secrete Granzyme B When Recognizing Viral Antigens in the Context of the Acute Phase Cytokine IL-21 | The Journal...Human B Cells Secrete Granzyme B When Recognizing Viral Antigens in the Context of the Acute Phase Cytokine IL-21 | The Journal...

Human B Cells Secrete Granzyme B When Recognizing Viral Antigens in the Context of the Acute Phase Cytokine IL-21. Magdalena ... Human B Cells Secrete Granzyme B When Recognizing Viral Antigens in the Context of the Acute Phase Cytokine IL-21 ... Human B Cells Secrete Granzyme B When Recognizing Viral Antigens in the Context of the Acute Phase Cytokine IL-21 ... Human B Cells Secrete Granzyme B When Recognizing Viral Antigens in the Context of the Acute Phase Cytokine IL-21 ...
more infohttp://www.jimmunol.org/content/early/2009/07/10/jimmunol.0901066

Pharmaq opens high-tech viral antigen production facility in Norway - AmCham NorwayPharmaq opens high-tech viral antigen production facility in Norway - AmCham Norway

On 1 November 2017, the Norwegian Medicines Agency granted Pharmaq authorisation to produce viral antigens at their new ...
more infohttps://amcham.no/pharmaq-opens-high-tech-viral-antigen-production-facility-norway/

Detection of a Herpes Simplex Viral Antigen in Skin Lesions of Erythema Multiforme | Annals of Internal Medicine | American...Detection of a Herpes Simplex Viral Antigen in Skin Lesions of Erythema Multiforme | Annals of Internal Medicine | American...

Detection of a Herpes Simplex Viral Antigen in Skin Lesions of Erythema Multiforme PAUL W. ORTON, M.D.; J. CLARK HUFF, M.D.; ... Detection of a Herpes Simplex Viral Antigen in Skin Lesions of Erythema Multiforme. Ann Intern Med. 1984;101:48-50. doi: ... viral antigen by an indirect immunofluorescence test using a monoclonal antibody to a major type-common glycoprotein antigen, ... Focal staining showed this antigen to be present in epidermal cells in 12 of 16 skin biopsy specimens. The staining was similar ...
more infohttps://annals.org/aim/article-abstract/698649/detection-herpes-simplex-viral-antigen-skin-lesions-erythema-multiforme

Radioimmunoassay of mammalian type C viral proteins. 3. Detection of viral antigen in normal murine cells and tissues.Radioimmunoassay of mammalian type C viral proteins. 3. Detection of viral antigen in normal murine cells and tissues.

The assay was shown to measure an antigen that is highly related to the ... the gs antigen, detects an antigenic reactivity in normal murine cells in culture and natural tissues. ... 0/Antigens, Viral; 0/Iodine Isotopes; 0/RNA, Viral; 0/Viral Proteins; 10028-17-8/Tritium; 50-89-5/Thymidine ... RADIOIMMUNOASSAY OF MAMMALIAN TYPE C VIRAL PROTEINS : III. DETECTION OF VIRAL ANTIGEN IN NORMAL MURINE CELLS AND TISSUES ...
more infohttp://www.biomedsearch.com/nih/Radioimmunoassay-mammalian-type-C-viral/4347595.html

Lung CD103+ Dendritic Cells Efficiently Transport Influenza Virus to the Lymph Node and Load Viral Antigen onto MHC Class I for...Lung CD103+ Dendritic Cells Efficiently Transport Influenza Virus to the Lymph Node and Load Viral Antigen onto MHC Class I for...

Lung CD103+ Dendritic Cells Efficiently Transport Influenza Virus to the Lymph Node and Load Viral Antigen onto MHC Class I for ... Lung CD103+ Dendritic Cells Efficiently Transport Influenza Virus to the Lymph Node and Load Viral Antigen onto MHC Class I for ... Lung CD103+ Dendritic Cells Efficiently Transport Influenza Virus to the Lymph Node and Load Viral Antigen onto MHC Class I for ... Lung CD103+ Dendritic Cells Efficiently Transport Influenza Virus to the Lymph Node and Load Viral Antigen onto MHC Class I for ...
more infohttp://www.jimmunol.org/content/187/11/6011.full

Project: Enhancing DNA vaccine efficacy by coexpression of viral antigen and cell death modulating proteinsProject: Enhancing DNA vaccine efficacy by coexpression of viral antigen and cell death modulating proteins

The aim of this project is to determine if the coexpression of a viral antigen and cell death modulating adaptor proteins can ... Project: Enhancing DNA vaccine efficacy by coexpression of viral antigen and cell death modulating proteins. project duration. ... enhance the immune response as well as protection against a homologous viral challenge.. ...
more infohttps://biblio.ugent.be/project/12CE0108

Viral hepatitis antigens | definition of viral hepatitis antigens by Medical dictionaryViral hepatitis antigens | definition of viral hepatitis antigens by Medical dictionary

What is viral hepatitis antigens? Meaning of viral hepatitis antigens medical term. What does viral hepatitis antigens mean? ... Looking for online definition of viral hepatitis antigens in the Medical Dictionary? viral hepatitis antigens explanation free ... viral hepatitis antigens. vi·ral hep·a·ti·tis an·ti·gens (vīrăl hepă-tītis anti-jĕnz) Immunogenic components of hepatitis ... Viral hepatitis antigens , definition of viral hepatitis antigens by Medical dictionary https://medical-dictionary. ...
more infohttps://medical-dictionary.thefreedictionary.com/viral+hepatitis+antigens

JCI -
Kinetics of protective antibodies are determined by the viral surface antigenJCI - Kinetics of protective antibodies are determined by the viral surface antigen

These findings indicate that the viral GP determines nAb kinetics largely independently of the specific viral infection context ... They further suggest that structural features of viral GPs or coevolutionary adaptation of the viruss GP to the hosts naive B ... Using a reverse genetic approach, we evaluated possible underlying mechanisms in 2 widely studied viral infection models. ... each of the 2 recombinant and parent viruses in infected mice and found that nAb kinetics were solely determined by the viral ...
more infohttps://jci.org/articles/view/22374/pdf

JCI -
Kinetics of protective antibodies are determined by the viral surface antigenJCI - Kinetics of protective antibodies are determined by the viral surface antigen

The nAbs of the indicated specificity were used for detection of the viral surface antigens. Scale bar: 0.2 μm. (. D. ) BHK-21 ... These findings indicate that the viral GP determines nAb kinetics largely independently of the specific viral infection context ... WT and recombinant viral genomes. (. A. ) The LCMV-ARM genome consists of 2 single-stranded negative-strand ambisense RNA ... They further suggest that structural features of viral GPs or coevolutionary adaptation of the viruss GP to the hosts naive B ...
more infohttps://jci.org/articles/view/22374/figure/1

Pathology and Viral Antigen Distribution of Lethal Pneumonia in Domestic Cats Due to Pandemic (H1N1) 2009 Influenza A VirusPathology and Viral Antigen Distribution of Lethal Pneumonia in Domestic Cats Due to Pandemic (H1N1) 2009 Influenza A Virus

... ... Pathology and viral antigen distribution of lethal pneumonia in domestic cats due to pandemic (H1N1) 2009 influenza A virus. ... influenza A viral antigen was demonstrated in bronchiolar epithelial cells, pneumocytes and alveolar macrophages in pneumonic ... the first description of the pathology and viral antigen distribution of lethal respiratory disease in domestic cats after ...
more infohttps://ir.library.oregonstate.edu/concern/defaults/8p58pd86s

Break of T cell ignorance to a viral antigen in the liver induces hepatitis  - Zurich Open Repository and ArchiveBreak of T cell ignorance to a viral antigen in the liver induces hepatitis - Zurich Open Repository and Archive

Download PDF Break of T cell ignorance to a viral antigen in the liver induces hepatitis. Item availability may be restricted ... Break of T cell ignorance to a viral antigen in the liver induces hepatitis ... Break of T cell ignorance to a viral antigen in the liver induces hepatitis. Journal of Immunology, 165(5):2415-2422. ...
more infohttps://www.zora.uzh.ch/id/eprint/10876/

Microbix in $25-million viral antigen supply pact | BioTuesdays by Kilmer LucasMicrobix in $25-million viral antigen supply pact | BioTuesdays by Kilmer Lucas

... customer with an increasing quantity of its viral antigen products over the next five years, with an option to extend that term ... Microbix, Microbix Biosystems, TSX, TSX:MBX, MBX, antigen, viral, global diagnostics company, infectious diseases, agreement, ... Microbix Biosystems (TSX:MBX) agreed to supply an existing long-term customer with an increasing quantity of its viral antigen ... with growing sales of infectious disease tests that require more antigen supply. Sales from the agreement are expected to total ...
more infohttp://www.biotuesdays.com/briefs/2017/8/7/microbix-in-25-million-viral-antigen-supply-pact
  • The two cases reported here provide, to the best of the authors' knowledge, the first description of the pathology and viral antigen distribution of lethal respiratory disease in domestic cats after natural pandemic (H1N1) 2009 influenza virus infection, probably transmitted from humans. (oregonstate.edu)
  • Even when a virus cannot be cultured, provided gene sequence is available, it is possible to rapidly respond to emerging viruses and new viral strains of existing pathogens. (prospecbio.com)
  • Mammalian Orthoreoviruses The only orthoreovirus to not produce syncytia, mammalian orthoreoviruses have the capability of infecting all mammals, but do not cause disease, except in young populations enabling them to be studied frequently as a model for viral replication and pathogenesis. (wikipedia.org)
  • They further suggest that structural features of viral GPs or coevolutionary adaptation of the virus's GP to the host's naive B cell repertoire, or both, may critically limit nAb kinetics and improvement of vaccine efficacy. (jci.org)
  • We analyzed the nAb response to each of the 2 recombinant and parent viruses in infected mice and found that nAb kinetics were solely determined by the viral surface GP and not by the virus backbone. (jci.org)
  • Systematic studies of immune responses to microbial antigens in vivo may be performed during vaccination. (nih.gov)
  • We observed augmented innate and adaptive immune responses in pSS following viral antigen exposure suggesting an underlying hyper-responsiveness to immune challenges, supporting a role for infections driving the immunopathology and acting as environmental risk factor for pSS. (nih.gov)
  • These findings suggest that the immune reaction and subsequent tissue damage of herpes-associated erythema multiforme are due to the presence of herpes antigens in the skin. (annals.org)
  • Microbix Biosystems (TSX:MBX) agreed to supply an existing long-term customer with an increasing quantity of its viral antigen products over the next five years, with an option to extend that term. (biotuesdays.com)
  • The immunodot blot test was used to identify African swine fever virus (ASFV) antigens in the hemolymph from soft ticks ( Ornithodoros coriaceus ) fed on ASFV-infected pigs. (ajtmh.org)
  • The immunodot blot test was sensitive and specific for ASFV antigens and has potential field application. (ajtmh.org)
  • Our data may also explain the elevated serum GrB levels found in the early phase of various viral diseases. (jimmunol.org)
  • On 1 November 2017, the Norwegian Medicines Agency granted Pharmaq authorisation to produce viral antigens at their new facility in Kløfta. (wfga.net)
  • Polyprotein is processed by host signalases, resulting in E3, E2 & E1 forming viral hetero-trimeric spikes. (prospecbio.com)