Plants, Genetically Modified
Plant Leaves
Plant Proteins
Plant Roots
Antigens, Neoplasm
Antigens, Surface
Plant Extracts
Plants, Medicinal
Plant Development
Plant Stems
Antigens, Protozoan
Antigens, Polyomavirus Transforming
Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.
HLA Antigens
Arabidopsis
Plants, Edible
An organism of the vegetable kingdom suitable by nature for use as a food, especially by human beings. Not all parts of any given plant are edible but all parts of edible plants have been known to figure as raw or cooked food: leaves, roots, tubers, stems, seeds, buds, fruits, and flowers. The most commonly edible parts of plants are FRUIT, usually sweet, fleshy, and succulent. Most edible plants are commonly cultivated for their nutritional value and are referred to as VEGETABLES.
Antigens, CD
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
Antigens, Helminth
Plant Growth Regulators
Carcinoembryonic Antigen
Antigens, Viral, Tumor
Arabidopsis Proteins
Amino Acid Sequence
Tobacco
Plant Immunity
HLA-DR Antigens
Receptors, Antigen, T-Cell
Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.
Histocompatibility Antigens
A group of antigens that includes both the major and minor histocompatibility antigens. The former are genetically determined by the major histocompatibility complex. They determine tissue type for transplantation and cause allograft rejections. The latter are systems of allelic alloantigens that can cause weak transplant rejection.
Proliferating Cell Nuclear Antigen
Nuclear antigen with a role in DNA synthesis, DNA repair, and cell cycle progression. PCNA is required for the coordinated synthesis of both leading and lagging strands at the replication fork during DNA replication. PCNA expression correlates with the proliferation activity of several malignant and non-malignant cell types.
Histocompatibility Antigens Class II
Large, transmembrane, non-covalently linked glycoproteins (alpha and beta). Both chains can be polymorphic although there is more structural variation in the beta chains. The class II antigens in humans are called HLA-D ANTIGENS and are coded by a gene on chromosome 6. In mice, two genes named IA and IE on chromosome 17 code for the H-2 antigens. The antigens are found on B-lymphocytes, macrophages, epidermal cells, and sperm and are thought to mediate the competence of and cellular cooperation in the immune response. The term IA antigens used to refer only to the proteins encoded by the IA genes in the mouse, but is now used as a generic term for any class II histocompatibility antigen.
O Antigens
The lipopolysaccharide-protein somatic antigens, usually from gram-negative bacteria, important in the serological classification of enteric bacilli. The O-specific chains determine the specificity of the O antigens of a given serotype. O antigens are the immunodominant part of the lipopolysaccharide molecule in the intact bacterial cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Prostate-Specific Antigen
Receptors, Antigen, B-Cell
Plant Epidermis
Base Sequence
Antigens, CD15
Antigens, Tumor-Associated, Carbohydrate
Plant Stomata
HLA-A2 Antigen
T-Lymphocytes
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
Antigens, CD8
Angiosperms
Members of the group of vascular plants which bear flowers. They are differentiated from GYMNOSPERMS by their production of seeds within a closed chamber (OVARY, PLANT). The Angiosperms division is composed of two classes, the monocotyledons (Liliopsida) and dicotyledons (Magnoliopsida). Angiosperms represent approximately 80% of all known living plants.
Enzyme-Linked Immunosorbent Assay
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Cross Reactions
Immunoglobulin G
Hepatitis B Surface Antigens
Blood Group Antigens
Antigens, CD3
Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).
HLA-A Antigens
Polymorphic class I human histocompatibility (HLA) surface antigens present on almost all nucleated cells. At least 20 antigens have been identified which are encoded by the A locus of multiple alleles on chromosome 6. They serve as targets for T-cell cytolytic responses and are involved with acceptance or rejection of tissue/organ grafts.
Histocompatibility Antigens Class I
Membrane glycoproteins consisting of an alpha subunit and a BETA 2-MICROGLOBULIN beta subunit. In humans, highly polymorphic genes on CHROMOSOME 6 encode the alpha subunits of class I antigens and play an important role in determining the serological specificity of the surface antigen. Class I antigens are found on most nucleated cells and are generally detected by their reactivity with alloantisera. These antigens are recognized during GRAFT REJECTION and restrict cell-mediated lysis of virus-infected cells.
Seeds
Antibody Specificity
Plant Poisoning
Lymphocyte Activation
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
HLA-D Antigens
Human immune-response or Class II antigens found mainly, but not exclusively, on B-lymphocytes and produced from genes of the HLA-D locus. They are extremely polymorphic families of glycopeptides, each consisting of two chains, alpha and beta. This group of antigens includes the -DR, -DQ and -DP designations, of which HLA-DR is most studied; some of these glycoproteins are associated with certain diseases, possibly of immune etiology.
Plant Transpiration
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Receptors, Antigen
Hepatitis B Antigens
Antigens, CD45
High-molecular weight glycoproteins uniquely expressed on the surface of LEUKOCYTES and their hemopoietic progenitors. They contain a cytoplasmic protein tyrosine phosphatase activity which plays a role in intracellular signaling from the CELL SURFACE RECEPTORS. The CD45 antigens occur as multiple isoforms that result from alternative mRNA splicing and differential usage of three exons.
Lycopersicon esculentum
Antigens, CD4
55-kDa antigens found on HELPER-INDUCER T-LYMPHOCYTES and on a variety of other immune cell types. CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in MAJOR HISTOCOMPATIBILITY COMPLEX class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as INTERLEUKIN-15 receptors and bind to the HIV receptors, binding directly to the HIV ENVELOPE PROTEIN GP120.
Fluorescent Antibody Technique
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Species Specificity
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Antigen-Antibody Reactions
Antigens, CD1
Plant Tumors
Immune Sera
Chromosomes, Plant
Antibody Formation
Mutation
HLA-B Antigens
Class I human histocompatibility (HLA) surface antigens encoded by more than 30 detectable alleles on locus B of the HLA complex, the most polymorphic of all the HLA specificities. Several of these antigens (e.g., HLA-B27, -B7, -B8) are strongly associated with predisposition to rheumatoid and other autoimmune disorders. Like other class I HLA determinants, they are involved in the cellular immune reactivity of cytolytic T lymphocytes.
Immunization
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
Antigens, Differentiation
Phenotype
B-Lymphocytes
MART-1 Antigen
Zea mays
HIV Antigens
Antigens, CD80
A costimulatory ligand expressed by ANTIGEN-PRESENTING CELLS that binds to CTLA-4 ANTIGEN with high specificity and to CD28 ANTIGEN with low specificity. The interaction of CD80 with CD28 ANTIGEN provides a costimulatory signal to T-LYMPHOCYTES, while its interaction with CTLA-4 ANTIGEN may play a role in inducing PERIPHERAL TOLERANCE.
Fabaceae
The large family of plants characterized by pods. Some are edible and some cause LATHYRISM or FAVISM and other forms of poisoning. Other species yield useful materials like gums from ACACIA and various LECTINS like PHYTOHEMAGGLUTININS from PHASEOLUS. Many of them harbor NITROGEN FIXATION bacteria on their roots. Many but not all species of "beans" belong to this family.
Biomass
Immunoenzyme Techniques
Epstein-Barr Virus Nuclear Antigens
Antigens, CD19
Plant Nectar
Sugar-rich liquid produced in plant glands called nectaries. It is either produced in flowers or other plant structures, providing a source of attraction for pollinating insects and animals, as well as being a nutrient source to animal mutualists which provide protection of plants against herbivores.
Antibodies
Cells, Cultured
Antigens, Heterophile
Oxylipins
Hepatitis B Core Antigens
Immunodiffusion
Autoantigens
Antigens, CD40
A member of the tumor necrosis factor receptor superfamily with specificity for CD40 LIGAND. It is found on mature B-LYMPHOCYTES and some EPITHELIAL CELLS, lymphoid DENDRITIC CELLS. Evidence suggests that CD40-dependent activation of B-cells is important for generation of memory B-cells within the germinal centers. Mutations of the gene for CD40 antigen result in HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 3. Signaling of the receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
Sequence Alignment
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Symbiosis
Plant Lectins
Protein or glycoprotein substances of plant origin that bind to sugar moieties in cell walls or membranes. Some carbohydrate-metabolizing proteins (ENZYMES) from PLANTS also bind to carbohydrates, however they are not considered lectins. Many plant lectins change the physiology of the membrane of BLOOD CELLS to cause agglutination, mitosis, or other biochemical changes. They may play a role in plant defense mechanisms.
Immunoglobulin M
Antigen-Antibody Complex
Photosynthesis
The synthesis by organisms of organic chemical compounds, especially carbohydrates, from carbon dioxide using energy obtained from light rather than from the oxidation of chemical compounds. Photosynthesis comprises two separate processes: the light reactions and the dark reactions. In higher plants; GREEN ALGAE; and CYANOBACTERIA; NADPH and ATP formed by the light reactions drive the dark reactions which result in the fixation of carbon dioxide. (from Oxford Dictionary of Biochemistry and Molecular Biology, 2001)
Antigens, Thy-1
Solanum tuberosum
Electrophoresis, Polyacrylamide Gel
Forssman Antigen
Flow Cytometry
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Recombinant Fusion Proteins
Indoleacetic Acids
H-Y Antigen
Soil
Rabbits
Antigen-Presenting Cells
A heterogeneous group of immunocompetent cells that mediate the cellular immune response by processing and presenting antigens to the T-cells. Traditional antigen-presenting cells include MACROPHAGES; DENDRITIC CELLS; LANGERHANS CELLS; and B-LYMPHOCYTES. FOLLICULAR DENDRITIC CELLS are not traditional antigen-presenting cells, but because they hold antigen on their cell surface in the form of IMMUNE COMPLEXES for B-cell recognition they are considered so by some authors.
Dendritic Cells
Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).
Complement Fixation Tests
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
HLA-DQ Antigens
Antigens, CD86
A costimulatory ligand expressed by ANTIGEN-PRESENTING CELLS that binds to CD28 ANTIGEN with high specificity and to CTLA-4 ANTIGEN with low specificity. The interaction of CD86 with CD28 ANTIGEN provides a stimulatory signal to T-LYMPHOCYTES, while its interaction with CTLA-4 ANTIGEN may play a role in inducing PERIPHERAL TOLERANCE.
Simian virus 40
Medicine, Traditional
T-Lymphocytes, Cytotoxic
Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.
Ecosystem
Salicylic Acid
Root Nodules, Plant
RNA, Messenger
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Receptors, Antigen, T-Cell, alpha-beta
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Immunoelectrophoresis
A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
Immunity, Cellular
Lymphocytes
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
DNA, Complementary
Mycorrhizae
Symbiotic combination (dual organism) of the MYCELIUM of FUNGI with the roots of plants (PLANT ROOTS). The roots of almost all higher plants exhibit this mutually beneficial relationship, whereby the fungus supplies water and mineral salts to the plant, and the plant supplies CARBOHYDRATES to the fungus. There are two major types of mycorrhizae: ectomycorrhizae and endomycorrhizae.
CTLA-4 Antigen
Interferon-gamma
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
Poaceae
Signal Transduction
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Chloroplasts
Plant cell inclusion bodies that contain the photosynthetic pigment CHLOROPHYLL, which is associated with the membrane of THYLAKOIDS. Chloroplasts occur in cells of leaves and young stems of plants. They are also found in some forms of PHYTOPLANKTON such as HAPTOPHYTA; DINOFLAGELLATES; DIATOMS; and CRYPTOPHYTA.
DNA Primers
Antigens, CD79
Plant Infertility
Brassica
Peptides
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
CA-19-9 Antigen
Hemagglutination Tests
Sensitivity and Specificity
Mice, Inbred Strains
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
Lewis Blood-Group System
A group of dominantly and independently inherited antigens associated with the ABO blood factors. They are glycolipids present in plasma and secretions that may adhere to the erythrocytes. The phenotype Le(b) is the result of the interaction of the Le gene Le(a) with the genes for the ABO blood groups.
CD4-Positive T-Lymphocytes
A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.
Fungi
A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies.
Germination
The initial stages of the growth of SEEDS into a SEEDLINGS. The embryonic shoot (plumule) and embryonic PLANT ROOTS (radicle) emerge and grow upwards and downwards respectively. Food reserves for germination come from endosperm tissue within the seed and/or from the seed leaves (COTYLEDON). (Concise Dictionary of Biology, 1990)
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
gp100 Melanoma Antigen
CD8-Positive T-Lymphocytes
Immunohistochemistry
Models, Biological
Ki-67 Antigen
Asteraceae
A large plant family of the order Asterales, subclass Asteridae, class Magnoliopsida. The family is also known as Compositae. Flower petals are joined near the base and stamens alternate with the corolla lobes. The common name of "daisy" refers to several genera of this family including Aster; CHRYSANTHEMUM; RUDBECKIA; TANACETUM.
Cell Wall
Antigens, T-Independent
Immune Tolerance
The specific failure of a normally responsive individual to make an immune response to a known antigen. It results from previous contact with the antigen by an immunologically immature individual (fetus or neonate) or by an adult exposed to extreme high-dose or low-dose antigen, or by exposure to radiation, antimetabolites, antilymphocytic serum, etc.
Antigens, CD2
Multigene Family
A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)
Molecular cloning and epitope analysis of the peanut allergen Ara h 3. (1/684)
Peanut allergy is a significant IgE-mediated health problem because of the increased prevalence, potential severity, and chronicity of the reaction. Following our characterization of the two peanut allergens Ara h 1 and Ara h 2, we have isolated a cDNA clone encoding a third peanut allergen, Ara h 3. The deduced amino acid sequence of Ara h 3 shows homology to 11S seed-storage proteins. The recombinant form of this protein was expressed in a bacterial system and was recognized by serum IgE from approximately 45% of our peanut-allergic patient population. Serum IgE from these patients and overlapping, synthetic peptides were used to map the linear, IgE-binding epitopes of Ara h 3. Four epitopes, between 10 and 15 amino acids in length, were found within the primary sequence, with no obvious sequence motif shared by the peptides. One epitope is recognized by all Ara h 3-allergic patients. Mutational analysis of the epitopes revealed that single amino acid changes within these peptides could lead to a reduction or loss of IgE binding. By determining which amino acids are critical for IgE binding, it might be possible to alter the Ara h 3 cDNA to encode a protein with a reduced IgE-binding capacity. These results will enable the design of improved diagnostic and therapeutic approaches for food-hypersensitivity reactions. (+info)Solution structure of a lipid transfer protein extracted from rice seeds. Comparison with homologous proteins. (2/684)
Nuclear magnetic resonance (NMR) spectroscopy was used to determine the three dimensional structure of rice nonspecific lipid transfer protein (ns-LTP), a 91 amino acid residue protein belonging to the broad family of plant ns-LTP. Sequence specific assignment was obtained for all but three HN backbone 1H resonances and for more than 95% of the 1H side-chain resonances using a combination of 1H 2D NOESY; TOCSY and COSY experiments at 293 K. The structure was calculated on the basis of four disulfide bridge restraints, 1259 distance constraints derived from 1H-1H Overhauser effects, 72 phi angle restraints and 32 hydrogen-bond restraints. The final solution structure involves four helices (H1: Cys3-Arg18, H2: Ala25-Ala37, H3: Thr41-Ala54 and H4: Ala66-Cys73) followed by a long C-terminal tail (T) with no observable regular structure. N-capping residues (Thr2, Ser24, Thr40), whose side-chain oxygen atoms are involved in hydrogen bonds with i + 3 amide proton additionally stabilize the N termini of the first three helices. The fourth helix involving Pro residues display a mixture of alpha and 3(10) conformation. The rms deviation of 14 final structures with respect to the average structure is 1.14 +/- 0.16 A for all heavy atoms (C, N, O and S) and 0.72 +/- 0.01 A for the backbone atoms. The global fold of rice ns-LTP is close to the previously published structures of wheat, barley and maize ns-LTPs exhibiting nearly identical pattern of the numerous sequence specific interactions. As reported previously for different four-helix topology proteins, hydrophobic, hydrogen bonding and electrostatic mechanisms of fold stabilization were found for the rice ns-LTP. The sequential alignment of 36 ns-LTP primary structures strongly suggests that there is a uniform pattern of specific long-range interactions (in terms of sequence), which stabilize the fold of all plant ns-LTPs. (+info)Production in Escherichia coli and site-directed mutagenesis of a 9-kDa nonspecific lipid transfer protein from wheat. (3/684)
The sequence encoding a wheat (Triticum durum) nonspecific lipid transfer protein of 9 kDa (nsLTP1) was inserted into an Escherichia coli expression vector, pET3b. The recombinant protein that was expressed accumulated in insoluble cytoplasmic inclusion bodies and was purified and refolded from them. In comparison with the corresponding protein isolated from wheat kernel, the refolded recombinant protein exhibits a methionine extension at its N-terminus but has the same structure and activity as demonstrated by CD, lipid binding and lipid transfer assays. Using the same expression system, four mutants with H5Q, Y16A, Q45R and Y79A replacements were produced and characterized. No significant changes in structure or activity were found for three of the mutants. By contrast, lipid binding experiments with the Y79A mutant did not show any increase of tyrosine fluorescence as observed with the wild-type nsLTP1. Comparison of the two tyrosine mutants suggested that Tyr79 is the residue involved in this phenomenon and thus is located close to the lipid binding site as expected from three-dimensional structure data. (+info)Two-dimensional electrophoresis of Malassezia allergens for atopic dermatitis and isolation of Mal f 4 homologs with mitochondrial malate dehydrogenase. (4/684)
The yeast Malassezia furfur is a natural inhabitant of the human skin microflora that induces an allergic reaction in atopic dermatitis. To identify allergens of M. furfur, we separated a crude preparation of M. furfur antigens as discrete spots by 2-D PAGE and detected IgE-binding proteins using sera of atopic dermatitis patients. We identified the known allergens, Mal f 2 and Mal f 3, and determined N-terminal amino acid sequences of six new IgE-binding proteins including Mal f 4. The cDNA and genomic DNA encoding Mal f 4 were cloned and sequenced. The gene was mitochondrial malate dehydrogenase and encoded Mal f 4 composed of 315 amino acids and a signal sequence of 27 amino acids. We purified Mal f 4, which had a molecular mass of 35 kDa from a membrane fraction of a lysate of cultured cells. Thirty of 36 M. furfur-allergic atopic dermatitis patients (83.3%) had elevated serum levels of IgE to purified Mal f 4, indicating that Mal f 4 is a major allergen. There was a significant correlation of the Phadebas RAST unit values of Mal f 4 and the crude antigen, but not between Mal f 4 and the known allergen Mal f 2. (+info)Molecular characterization of American cockroach tropomyosin (Periplaneta americana allergen 7), a cross-reactive allergen. (5/684)
Inhalation of allergens produced by the American cockroach (Periplaneta americana) induces IgE Ab production and the development of asthma in genetically predisposed individuals. The cloning and expression in Escherichia coli of P. americana tropomyosin allergen have been achieved. The protein shares high homology with other arthropod tropomyosins (80% identity) but less homology with vertebrate ones (50% identity). The recombinant allergen was produced in E. coli as a nonfusion protein with a yield of 9 mg/l of bacterial culture. Both natural and recombinant tropomyosins were purified by isoelectric precipitation. P. americana allergen 1 (Per a 1) and Per a 7 (tropomyosin) are to date the only cross-reacting allergens found in cockroaches. ELISA and Western blot inhibition experiments, using natural and recombinant purified tropomyosins from shrimp and cockroach, showed that tropomyosin induced cross-reactivity of IgE from patients allergic to these allergens, suggesting that this molecule could be a common allergen among invertebrates. (+info)Molecular dissection of mitogillin reveals that the fungal ribotoxins are a family of natural genetically engineered ribonucleases. (6/684)
Mitogillin and the related fungal ribotoxins are highly specific ribonucleases which inactivate the ribosome enzymatically by cleaving the 23-28 S RNA of the large ribosomal subunit at a single phosphodiester bond. The site of cleavage occurs between G4325 and A4326 (rat ribosome numbering) which are present in one of the most conserved sequences (the alpha-sarcin loop) among the large subunit ribosomal RNAs of all living species. Amino acid sequence comparison of ribotoxins and guanyl/purine ribonucleases have identified domains or residues likely involved in ribonucleolytic activity or cleavage specificity. Fifteen deletion mutants (each 4 to 8 amino acid deletions) in motifs of mitogillin showing little amino acid sequence homology with guanyl/purine ribonucleases were constructed by site-directed mutagenesis. Analyses of the purified mutant proteins identified those regions in fungal ribotoxins contributing to ribosome targeting and modulating the catalytic activity of the toxin; some of the identified motifs are homologous to sequences in ribosomal proteins and elongation factors. This mutational study of mitogillin together with the recently published x-ray structure of restrictocin (a close relative of mitogillin) supports the hypothesis that the specific cleavage properties of ribotoxins are the result of natural genetic engineering in which the ribosomal targeting elements of ribosome-associated proteins were inserted into nonessential regions of T1-like ribonucleases. (+info)Production and detailed characterization of biologically active olive pollen allergen Ole e 1 secreted by the yeast Pichia pastoris. (7/684)
The glycoprotein Ole e 1 is a significant aeroallergen from the olive tree (Olea europaea) pollen, with great clinical relevance in the Mediterranean area. To produce a biologically active form of recombinant Ole e 1, heterologous expression in the methylotrophic yeast Pichia pastoris was carried out. A cDNA encoding Ole e 1, fused to a Saccharomyces cerevisiae alpha-mating factor prepropeptide using the pPIC9 vector, was inserted into the yeast genome under the control of the AOX1 promoter. After induction with methanol, the protein secreted into the extracellular medium was purified by ion-exchange and size-exclusion chromatography. The structure of the isolated recombinant Ole e 1 was determined by chemical and spectroscopic techniques, and its immunological properties analysed by blotting and ELISA inhibition with Ole e 1-specific monoclonal antibodies and IgE from sera of allergic patients. The allergen was produced at a yield of 60 mg per litre of culture as a homogeneous glycosylated protein of around 18.5 kDa. Recombinant Ole e 1 appears to be properly folded, as it displays spectroscopic properties (CD and fluorescence) and immunological reactivities (IgG binding to monoclonal antibodies sensitive to denaturation and IgE from sera of allergic patients) indistinguishable from those of the natural protein. This approach gives high-yield production of homogeneous and biologically active allergen, which should be useful for scientific and clinical purposes. (+info)Sequence-divergent units of the ABA-1 polyprotein array of the nematode Ascaris suum have similar fatty-acid- and retinol-binding properties but different binding-site environments. (8/684)
Polyproteins comprise long polypeptides that are post-translationally cleaved into proteins of different function, or tandemly repetitive polypeptides which are processed into multiple versions of proteins which are presumed to have the same function. In the latter case the individual units of the polyprotein can differ substantially in sequence. Identity of function between the different units therefore cannot be assumed. Here we have examined the ABA-1 polyprotein allergen of the parasitic nematode Ascaris suum and found it to contain units which show a 50% difference in amino acid sequence. The parasite therefore produces at least two radically different forms of the allergen encoded within the polyprotein array. In fluorescence-based ligand-binding assays, recombinant polypeptides representing the two forms (designated ABA-1A1 and ABA-1B1) showed similar binding affinities for a range of fluorescent active-site probes [retinol, dansylundecanoic acid, dansyl-DL-alpha-amino-octanoic acid, cis-parinaric acid (cPnA)] and for the non-specific hydrophobic surface probe 8-anilinonaphthalene-1-sulphonic acid. However, the molecular environments in the active sites are markedly different, as indicated by disparate fluorescence emission peaks and intensities of bound probes. CD showed that the proteins have similar secondary structures but differ in susceptibility to chemical denaturation/unfolding by guanidinium chloride. Both retain a single conserved tryptophan residue in a characteristic non-polar environment, as revealed by extreme fluorescence blue shift. Thus the gross differences in sequence of the two proteins are not reflected in their ligand-binding specificities but in their binding-site environments. (+info)
The major olive pollen allergen (Ole e I) shows both gametophytic and sporophytic expression during anther development, and its...
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Pattern recognition receptor
This family of proteins is greatly expanded in plants, and constitutes a core component of plant immune systems. The ligands ... PRRs also mediate the initiation of antigen-specific adaptive immune response and release of inflammatory cytokines. The ... Upon ligand recognition, the plant PRRs transduce "PAMP-triggered immunity" (PTI). Plant immune systems also encode resistance ... PRRs were first discovered in plants. Since that time many plant PRRs have been predicted by genomic analysis (370 in rice; 47 ...
Genome Valley
viral antigen plant". The Hindu. -. 26 November 2020. ISSN 0971-751X. Retrieved 11 January 2021.CS1 maint: others (link) ... IIL is the market leader in veterinary biologicals in India and operates one of the largest plants in the world for veterinary ... Ashland A Fortune 500 specialty chemicals company operates R&D lab and pilot plant space in Genome Valley. Envigo Envigo is a ... Innopolis offers leasing opportunities for wet / dry laboratory spaces, specialized office and pilot plant spaces for Life- ...
History of genetic engineering
X-rays were first used to deliberately mutate plants in 1927. Between 1927 and 2007, more than 2,540 genetically mutated plant ... Gentner, B.; Naldini, L. (2012-11-01). "Exploiting microRNA regulation for genetic engineering". Tissue Antigens. 80 (5): 393- ... The first field trials of genetically engineered plants occurred in France and the US in 1986, tobacco plants were engineered ... Hybridization was another way that rapid changes in plant's makeup were introduced. It often increased vigor in plants, and ...
Edestin
Galasso, Incoronata (2016), "Variability in Seed Traits in a Collection of Cannabis sativa L. Genotypes", Frontiers in Plant ... as well as toxins or antigens as they enter the body). Edestin can also be broken down to edestan. Commercial hemp seeds (for ... Teresa Docimo (September 24, 2014). "Molecular characterization of edestin gene family in Cannabis sativa L". Plant Physiology ... "Immunological responses to Salmonella R antigens. The bacterial cell and the protein edestin as carriers for R oligosaccharide ...
Immunologic adjuvant
The plant extract QS-21 is a liposome made up of plant saponins from Quillaja saponaria, the soap bark tree. It is a part of ... Second, adjuvants may provide physical protection to antigens which grants the antigen a prolonged delivery. This means the ... or enhance antigen-specific immune responses when used in combination with specific vaccine antigens." In the early days of ... No matter what the mechanism is, alum is not a perfect adjuvant because it does not work with all antigens (e.g. malaria and ...
Arthrographis kalrae
A. kalrae secretes antigens with haemolytic and cytotoxic activity. Additionally, scientists use mice as animal model to study ... Arthrographis kalrae is capable of pathogenicity in animals (including humans) but not in plants. Most human isolates of A. ... LA, nagashima (2014). "Arthrographis Kalrae soluble antigens present hemolytic and cytotoxic actitvities". Comp Immunol ...
Edible vaccines
The antigen concentration can also vary significantly between individual fruits on a plant, individual plants, and between ... The dosage also varies due to the difficulty in standardizing the concentration of the antigen in the plant tissue; it can be ... The entire gene is inserted into a plant transformation vector to allow transcription or the epitope within the antigen is ... The plants take up the DNA, grow into new plants, then are cloned to produce large numbers of genetically identical crops. The ...
Lab-on-a-chip
Specifically, plant on a chip is a miniaturized device in which pollen tissues and ovules could be incubated for plant sciences ... Immunoassay: detect bacteria, viruses and cancers based on antigen-antibody reactions. Ion channel screening (patch clamp) ... "A microsystem-based assay for studying pollen tube guidance in plant reproduction". J. Micromech. Microeng. 25 (5): 054018. ...
ENOX2
"Entrez Gene: COVA1 cytosolic ovarian carcinoma antigen 1". Morré, D. James; Morré, Dorothy M. (1998-11-01). "NADH oxidase ... 24 min". In Vitro Cellular & Developmental Biology - Plant. 37 (1): 19-23. doi:10.1007/s11627-001-0004-3. ISSN 1054-5476. PMID ... activity of soybean plasma membranes oscillates with a temperature compensated period of 24 min". The Plant Journal. 16 (3): ...
Vaccine
"Expression of a ScFv-E2T fusion protein in CHO-K1 cells and alfalfa transgenic plants for the selective directioning to antigen ... Dendritic cell vaccines combine dendritic cells with antigens in order to present the antigens to the body's white blood cells ... The idea of vaccine production via transgenic plants was identified as early as 2003. Plants such as tobacco, potato, tomato, ... Another example is the expression of a fusion protein in alfalfa transgenic plants for the selective directioning to antigen ...
Nepenthes alata
Carnivorous Plant Database. Smythies, B.E. 1965. The distribution and ecology of pitcher-plants (Nepenthes) in Sarawak. UNESCO ... The effect of the Nepenthes alata extract on the cold agglutinin-associated antigens. Nihon Hōigaku Zasshi 45(1): 30-32. PMID ... Carnivorous Plant Newsletter 19(1-2): 19-28. Shivas, R.G. 1984. Pitcher Plants of Peninsular Malaysia & Singapore. Maruzen Asia ... Carnivorous Plants in the tropics, January 12, 2011. Robinson, A. 2012. Nepenthes merrilliana on Samar. Carnivorous Plants in ...
Pathosystem
In breeding crop plants for horizontal resistance to their parasites, the disciplines of plant breeding, plant pathology, and ... The gene-for-gene relationship is an approximate botanical equivalent of antigens and antibodies in mammals. For each ... A plant pathosystem is one in which the host species is a plant. The parasite is any species in which the individual spends a ... It brings together various crop science disciplines such as entomology, nematology, plant pathology, and plant breeding. It ...
Proteolipid
Li Y, Qi B (2017). "Progress toward Understanding Protein S-acylation: Prospective in Plants". Frontiers in Plant Science. 8: ... These proteins are also potent antigens, and are in fact the main immunogens of these two species. Products of nonribosomal ... The plant chloroplast is capable of many of the same modifications that bacteria perform to proteolipids. One database for such ... Proteolipids are abundant in brain tissue, and are also present in many other animal and plant tissues. They are proteins ...
Robert Purcell (virologist)
The same team who co-identified the Hepatitis A virus (HAV) developed the first assays that could measure the virus antigen and ... Hepatitis C virus shares amino acid sequence similarity with pestiviruses and flaviviruses as well as members of two plant ... detection by immune electron microscopy of a viruslike antigen associated with illness. Science. 182(4116): pgs. 1026-1028 " ...
Asparagine endopeptidase
Müntz K, Shutov AD (August 2002). "Legumains and their functions in plants". Trends in Plant Science. 7 (8): 340-4. doi:10.1016 ... Antigen presenting is a key role in activation of immune system. It has been discovered that AEP plays role in this critical ... "Biosynthesis of circular proteins in plants". The Plant Journal. 53 (3): 505-15. doi:10.1111/j.1365-313x.2007.03357.x. PMID ... In plant Oldenlandia affinis it generates antimicrobial cyclic peptides which are important for defence against pathogens in ...
Sugar
It is a biennial plant,[78] a cultivated variety of Beta vulgaris in the family Amaranthaceae, the tuberous root of which ... It is a component of the antigens found on the surface of red blood cells that determine blood groups.[62] ... Table 1. Sugar content of selected common plant foods (g/100g)[72] Food item Total. carbohydrateA. including. dietary fiber ... Lactose is the only sugar that cannot be extracted from plants. It can only be found in milk, including human breast milk, and ...
RNA splicing
In plants, variation for flooding stress tolerance correlated with stress-induced alternative splicing of transcripts ... post-translational protein splicing and other lessons from the school of antigen processing". Journal of Molecular Medicine. 83 ... Protein splicing has been observed in a wide range of organisms, including bacteria, archaea, plants, yeast and humans. cDNA ... Plant Physiology. 172 (2): 668-89. doi:10.1104/pp.16.00472. PMC 5047075. PMID 27208254. Hanada K, Yang JC (June 2005). "Novel ...
Cryptosporidiosis
Detecting antigens is yet another way to diagnose the disease. This can be done with direct fluorescent antibody (DFA) ... Many treatment plants that take raw water from rivers, lakes, and reservoirs for public drinking water production use ... Antibodies in the serum of humans and animals infected with Cryptosporidium parvum react with several antigens, one of which is ... Equipment failures at the city's antiquated water filtration plant following maintenance were found to have caused the outbreak ...
Microtechnique
Plant microtechnique is also a study providing valuable experimental information. Plant microtechnique involves classical ... This method can preserve the immune activity of various antigens well. Both fresh tissue and fixed tissue can be frozen. ... Main article: Histology Since few plant tissues have a color, there is little chromatically difference between plant tissues ... 69-89, doi:10.1007/978-1-4684-0118-9_2, ISBN 9781468401189 "Plant Anatomy_Free Hand Sectioning , Microscope , Plant Stem". ...
Frequency-dependent selection
When two plants share the same incompatibility allele, they are unable to mate. Thus, a plant with a new (and therefore, rare) ... The major histocompatibility complex (MHC) is involved in the recognition of foreign antigens and cells. Frequency-dependent ... Another example is plant self-incompatibility alleles. ...
Hazard
The US Environmental Protection Agency (EPA) controls GM plants that produce or resist pesticides (i.e. Bt corn and Roundup ... Disease in humans can come from biological hazards in the form of infection by bacteria, antigens, viruses, or parasites. ... Pesticides, which are normally used to control unwanted insects and plants, may cause a variety of negative effects on non- ... Many biological hazards are associated with food, including certain viruses, parasites, fungi, bacteria, and plant and seafood ...
Pentamer
In immunology, an MHC pentamer is a reagent used to detect antigen-specific CD8+ T cells. penta prefix -mer suffix Pentamerous ... Metamorphosis, an album by Global Communication Pentamery (botany), having five parts in a distinct whorl of a plant structure ...
Virus-like particle
VLPs derived from the Hepatitis B virus (HBV) and composed of the small HBV derived surface antigen (HBsAg) were described in ... Santi L, Huang Z, Mason H (September 2006). "Virus-like particles production in green plants". Methods. 40 (1): 66-76. doi: ... RTS,S is a portion of the Plasmodium falciparum circumsporozoite protein fused to the Hepatitis B surface antigen (RTS), ... Bayer ME, Blumberg BS, Werner B (June 1968). "Particles associated with Australia antigen in the sera of patients with ...
Novartis
... and manufacturing plants. It will also look at improving manufacturing of Chimeric antigen receptor T cell (CAR T cells). ... The plant ceased operation in 1996. A follow-up study from the 1996-2000 period indicated that while there were more cancer ... Dalton, Rex (1 November 2003). "Syngenta ends plant-research deal with Berkeley". Nature. 426 (6964): 216. Bibcode:2003Natur. ... a fire broke out in a production plant storage room, which led to the Sandoz chemical spill and a large amount of pesticide ...
DNA clamp
"Highly conserved structure of proliferating cell nuclear antigen (DNA polymerase delta auxiliary protein) gene in plants". ... The sequence of PCNA is well conserved between plants, animals and fungi, indicating a strong selective pressure for structure ... Proliferating cell nuclear antigen from Pyrococcus furiosus". Protein Sci. 10 (1): 17-23. doi:10.1110/ps.36401. PMC 2249843. ... "Structural and biochemical studies of human proliferating cell nuclear antigen complexes provide a rationale for cyclin ...
Phytomonas
Inhabits the fruit of tomato plants. This species has antigens that are very similar to some antigens of Trypanosoma cruzi, the ... Within a host plant, Phytomonas exhibits a fusiform structure twisted 2-5 times along the longitudinal axis. Within the plant, ... It was found that T. cruzi shares some antigens with P. serpens, and that these antigens could be recognized by rabbit and ... In English, the term phyto- means plant, and comes from the Greek term for plant, phuton. The suffix -monas means "unicellular ...
ELISA
The labeled antigen competes for primary antibody binding sites with the sample antigen (unlabeled). The less antigen in the ... ELISA has been used as a diagnostic tool in medicine, plant pathology, and biotechnology, as well as a quality control check in ... The antigen-containing sample is applied to the plate, and captured by antibody. The plate is washed to remove unbound antigen ... Since it is necessary to remove any unbound antibody or antigen by washing, the antibody or antigen has to be fixed to the ...
Circular permutation in proteins
The PSI is a domain exclusively found in plants, consisting of approximately 100 residues and found in plant aspartic proteases ... Saposins are a class of proteins involved in sphingolipid catabolism and antigen presentation of lipids in humans. Chris ... This repeat suggests a mechanism for the evolution of the relationship with the plant-specific insert (PSI). ... Ponting and Robert Russell identified a circularly permuted version of a saposin inserted into plant aspartic proteinase, which ...
Ebola
"First Antigen Rapid Test for Ebola through Emergency Assessment and Eligible for Procurement". World Health Organization (WHO ... Of 24 plant and 19 vertebrate species experimentally inoculated with EBOV, only bats became infected.[86] The bats displayed no ... a rapid antigen test which gives results in 15 minutes was approved for use by WHO.[101] It is able to confirm Ebola in 92% of ... Plants, arthropods, rodents, and birds have also been considered possible viral reservoirs.[1][29] ...
Wikipedia:Reference desk/Science
Plants - berry and juices questions.[edit]. Strawberries, raspberries, and blackberries roughly have the same lifespan, they ... I've gotten the vaccine so I don't know how good antigen tests would be. Also, home test kits have high error rates. Imagine ... similar to hydroelectric power plant? Or would the costs of maintaining the superfluidity be too high? 212.180.235.46 (talk) 14 ...
Evolution of biological complexity
Pays, E. (2005). "Regulation of antigen gene expression in Trypanosoma brucei". Trends Parasitol. 21 (11): 517-20. doi:10.1016/ ... has evolved so many copies of its major surface antigen that about 10% of its genome is devoted to different versions of this ...
Lyme disease
OspA antigens, shed by live Borrelia bacteria into urine, are a promising technique being studied.[117] The use of nanotrap ... ornamental plants and perennial groundcover (about a quarter), and lawns (about 30 times less).[130] Ixodes larvae and nymphs ... The CDC does not recommend urine antigen tests, PCR tests on urine, immunofluorescent staining for cell-wall-deficient forms of ... burgdorferi sensu stricto antigens in people have been identified in Colombia,[237] and Bolivia.[citation needed] ...
RAD51
In vertebrates and plants, five paralogs of RAD51 are expressed in somatic cells, including RAD51B (RAD51L1), RAD51C (RAD51L2 ... Outside of plants and vertebrates, a much broader diversity of Rad51 recombinase paralog proteins exists. In budding yeast, ...
Retrovirus
Group-specific antigen (gag) proteins are major components of the viral capsid, which are about 2000-4000 copies per virion. ... Plant virus (Plant to Human). *Animal virus. *Human virome. Other. *Viral disease ...
Pasteur Institute
... as an antigen, Richard F. J. Pfeiffer introduced it in the abdomen of a guinea pig already vaccinated against this disease, and ... Bernard's posthumous essay regarding the nature of the agents implicated in some transformations that happen inside plants, ... and they deduced that it can play the role of antigen, that is if they could overcome the delicate moment of its injection, ...
Innate immune system - Simple English Wikipedia, the free encyclopedia
It is the main immune system found in plants, fungi, insects, and in primitive multicellular organisms.[2][3] The system is not ... rid the body of neutralized antigen-antibody complexes.. Elements of the complement cascade can be found in many non-mammalian ... Innate immune systems rapidly defend against infections in all plant and animal life.[2] The innate system is the ... Activates the adaptive immune system through a process known as antigen presentation. ...
Proteasome
Regulation of plant growthEdit. In plants, signaling by auxins, or phytohormones that order the direction and tropism of plant ... Peptide antigens are displayed by the major histocompatibility complex class I (MHC) proteins on the surface of antigen- ... Dharmasiri S, Estelle M (2002). "The role of regulated protein degradation in auxin response". Plant Molecular Biology. 49 (3-4 ... Sadanandom A, Bailey M, Ewan R, Lee J, Nelis S (October 2012). "The ubiquitin-proteasome system: central modifier of plant ...
Innate immune system
Most plant immune responses involve systemic chemical signals sent throughout a plant. Plants use pattern-recognition receptors ... Normal body cells are not recognized and attacked by NK cells because they express intact self MHC antigens. Those MHC antigens ... Plants[edit]. Main article: Plant disease resistance § Immune system. Members of every class of pathogen that infect humans ... and insects can all cause plant disease. As with animals, plants attacked by insects or other pathogens use a set of complex ...
Glossary of biology
2. In botany, the property of a plant or plant part that is attached directly by its base to an object or another plant part, i ... of the immune system in response to specific antigens invading the body. The theory has become the widely accepted model for ... A type of plant tissue responsible for the transport of water from roots to aerial parts of the plant.. ... Biomass can be used as a source of energy and it most often refers to plants or plant-based materials which are not used for ...
Glycosylation
"Transgenic plants of Nicotiana tabacum L. express aglycosylated monoclonal antibody with antitumor activity". Biotecnologia ... It is the presence or absence of glycosyltransferases which dictates which blood group antigens are presented and hence what ...
Food allergy
1 - antigen. 2 - IgE antibody. 3 - FcεRI receptor. 4 - preformed mediators (histamine, proteases, chemokines, heparin). 5 - ... "Assessment of endogenous allergenicity of genetically modified plants exemplified by soybean - Where do we stand?" (PDF). Food ... Other widely promoted tests such as the antigen leukocyte cellular antibody test and the food allergy profile are considered ...
Plasmodium knowlesi
... and many West Africans lack the Duffy antigen - a protein on the surface of the red blood cell that the parasite uses to invade ... Plant. Hacrobia. Heterokont. Alveolata. Rhizaria. Excavata. Amoebozoa. Opisthokonta Animal. Fungi). Acavomonidia. Acavomonadea ...
Kidney disease
In order to be a match for a kidney transplant, patients must match blood type and human leukocyte antigen factors with their ... Plant Foods for Human Nutrition (Dordrecht, Netherlands). 65 (1): 77-82. doi:10.1007/s11130-009-0149-5. ISSN 1573-9104. PMID ...
Epigenetics
... plant cells do not terminally differentiate, remaining totipotent with the ability to give rise to a new individual plant. ... has a 10-40-fold preference for hemimethylated DNA and interacts with the proliferating cell nuclear antigen (PCNA).[51] ... While plants do utilise many of the same epigenetic mechanisms as animals, such as chromatin remodeling, it has been ... miRNAs regulate a large variety of biological functions in plants and animals.[64] So far, in 2013, about 2000 miRNAs have been ...
Lasker Award
Discoveries that revealed an unanticipated world of tiny RNAs that regulate gene function in plants and animals.[32] ... cells-the preeminent component of the immune system that initiates and regulates the body's response to foreign antigens.[34] ...
Kevin Cooper (prisoner)
Judge Fletcher suggested that preservatives found in the blood on the tan T-shirt indicated that it may have been planted ( ... and when Judge Huff did order retesting she limited the testing to hairs that were proven not to have Antigen roots ( ... 7] Some argue that Gregonis planted Cooper's blood in order to ensure a positive result.[7] ... Judge Fletcher writes, "Some of those cigarette butts could have easily been planted in the car. Moreover, after initial ...
Antigenic drift
If one of these new forms of an antigen is sufficiently different from the old antigen, it will no longer bind to the receptors ... Two processes drive the antigens to change: antigenic drift[1][2] and antigenic shift, antigenic drift being the more common. ... In the influenza virus, the two relevant antigens are the surface proteins, hemagglutinin and neuraminidase.[4] The ... A. W. Hampson (2002). "Influenza virus antigens and antigenic drift". In C. W. Potter. Influenza. Elsevier Science B. V. pp. 49 ...
Hepadnaviridae
... surface antigens-preS1, preS2, and S, the X protein and HBeAg. The X protein is thought to be non-structural. Its function and ... Plant virus (Plant to Human). *Animal virus. *Human virome. *Archea virus. *Amoeba virus ...
Protein
Antibodies are protein components of an adaptive immune system whose main function is to bind antigens, or foreign substances ... Most microorganisms and plants can biosynthesize all 20 standard amino acids, while animals (including humans) must obtain some ... Ribbon diagram of a mouse antibody against cholera that binds a carbohydrate antigen ... Dogs and cats digest animal proteins better than those from plants but products of low-quality animal origin are poorly ...
Rosetta Stone
The flowering plant Arabidopsis thaliana has been called the "Rosetta Stone of flowering time".[90] A Gamma ray burst (GRB) ... Fully understanding the key set of genes to the human leucocyte antigen has been described as "the Rosetta Stone of immunology ...
Allergic rhinitis
Pollens of insect-pollinated plants are too large to remain airborne and pose no risk. Examples of plants commonly responsible ... A major antigen in these is a protein called Bet V I. Olive pollen is most predominant in Mediterranean regions. Hay fever in ... Allergic rhinitis triggered by the pollens of specific seasonal plants is commonly known as "hay fever", because it is most ... Pollen grains from a variety of plants, enlarged 500 times and about 0.4 mm wide. ...
Lektiny - Wikipedie
GalNAc and α-D-GalNAc-Ser/Thr (Tn-antigen) WFA Wisteria floribunda vistárie mnohokvětá koncové GalNAc (např. β-D-GalNAc-(1-4)- ... A new type of beta-propeller architecture formed by oligomerization and interacting with fucoside, fucosyllactose, and plant ... β-D-Gal-(1-3)-α-D-GalNAc-Ser/Thr (T-antigen) ... Biological effects of plant lectins.... *World of Lectin links ... recognition of high mannose-type N-glycans produced by plants and yeast."Glycoconj J. 2005 Nov;22(7-9):453-61. ...
Non-celiac gluten sensitivity
There is evidence that not only gliadin (main cytotoxic antigen of gluten), but also other proteins present in gluten and ... ATIs are part of the plant's natural defense against insects and may cause toll-like receptor 4 (TLR4)-mediated intestinal ...
Cutaneous leishmaniasis
Alternatively, these plants may serve to capture and control sand flies by using their odor compounds or the plants themselves ... immunodiagnosis of parasite antigen; 3. detection of parasite DNA in tissue. Newer PCR based tools have higher sensitivity and ... Studies conducted in recent years show that the plant Bougainvillea glabra may protect against the sand fly . The plant was ... Hebrew University study found that some plants attract sand flies. These plants often attract sand flies up to 14 times more ...
Pandemic H1N1/09 virus
A study from Hokkaido University found a homology between the Hemagglutinin antigen amino acid residues found in the earlier ... that the virus may be the product of three strains from three continents that swapped genes in a lab or a vaccine-making plant ...
Colonia Tovar
... plants, candy and crafts. Tovar also produces quality wooden casks, which are famous in and out of the mountain. Los colonieros ... "Tissue Antigens. 62 (5): 401-407. ISSN 0001-2815.. .mw-parser-output cite.citation{font-style:inherit}.mw-parser-output . ...
Secretion
The Hrp system in plant pathogens inject harpins and pathogen effector proteins through similar mechanisms into plants. This ... One such mechanism to detect low calcium concentration has been illustrated by the lcrV (Low Calcium Response) antigen utilized ... Since then, Type VI secretion systems have been found in a quarter of all proteobacterial genomes, including animal, plant, ... which uses this system to introduce the T-DNA portion of the Ti plasmid into the plant host, which in turn causes the affected ...
Expression of hepatitis B surface antigen in transgenic banana plants | SpringerLink
Rasthali (AAB) have been transformed with the s gene of hepatitis B surface antigen (HBsAg) using Agrobacteriummediated ... The expression levels of the antigen in the plants grown under in vitro conditions as well as the green house hardened plants ... Mason HS, Lam DMK, Arntzen CJ (1992) Expression of hepatitis B surface antigen in transgenic plants. Proc Nat Acad Sci USA 89: ... Richter LJ, Thanavala Y, Arntzen CJ, Mason HS (2000) Production of hepatitis B surface antigen in transgenic plants for oral ...
Antigens, plant financial definition of Antigens, plant
What is Antigens, plant? Meaning of Antigens, plant as a finance term. What does Antigens, plant mean in finance? ... plant in the Financial Dictionary - by Free online English dictionary and encyclopedia. ... Antigens, plant financial definition of Antigens, plant https://financial-dictionary.thefreedictionary.com/Antigens%2c+plant ... plant. (redirected from Antigens, plant). Also found in: Dictionary, Thesaurus, Medical, Encyclopedia. Plant. The assets of a ...
A Plant-Produced Antigen Elicits Potent Immune Responses against West Nile Virus in Mice
Characterization of Antiserum against Plant-Derived DIII Antigen. Antisera obtained at week 11 from mice of the 25 μg plant- ... A Plant-Produced Antigen Elicits Potent Immune Responses against West Nile Virus in Mice. Junyun He,1 Li Peng,1 Huafang Lai,1 ... For example, we used nontransgenic N. benthamiana plants for DIII production in this study. As a result, the wild-type plant ... Plant-Produced DIII Elicits Potent Systemic Immune Response in Mice. To evaluate the immunogenicity of plant-derived DIII, BALB ...
Characterization of plant XRCC1 and its interaction with proliferating cell nuclear antigen
Thus, the plant short-patch base excision repair (short-patch BER) pathway must differ considerably from that in mammals. We ... In plants, there are no DNA polymerase beta (Pol beta) and DNA ligase III (Lig3) genes. ... Characterization of plant XRCC1 and its interaction with proliferating cell nuclear antigen Planta. 2008 May;227(6):1233-41. ... In plants, there are no DNA polymerase beta (Pol beta) and DNA ligase III (Lig3) genes. Thus, the plant short-patch base ...
GH Protein Human Plant | Somatotropin Antigen | ProSpec
Arabidopsis thaliana plants expressing Rift Valley fever virus antigens : Mice exhibit systemic immune responses as the result...
Furthermore, the plant-expressed antigens were evaluated for their immunogenicityin mice fed the transgenic plants. After oral ... Arabidopsis thaliana plants expressing Rift Valley fever virus antigens: Mice exhibit systemic immune responses as the result ... Antigen production, Arabidopsis thaliana, Rift valley fever virus, Plant vaccine, Transformation National Category Immunology ... Here, we report the expression in transformed plants (Arabidopsis thaliana) of Rift Valley fever virusantigens. The antigens ...
Frontiers | Antigen Production in Plant to Tackle Infectious Diseases Flare Up: The Case of SARS | Plant Science
For the M protein, this is the first description of production in plants, while for plant-derived N protein we demonstrate that ... The availability of recombinant N and M proteins from plants opens the way to further evaluation of their potential utility for ... The availability of recombinant N and M proteins from plants opens the way to further evaluation of their potential utility for ... while for plant-derived N protein we demonstrate that it is recognized by sera of patients from the SARS outbreak in Hong Kong ...
Frontiers | Transient Expression of Dengue Virus NS1 Antigen in Nicotiana benthamiana for Use as a Diagnostic Antigen | Plant...
Plant-based systems can be a safe and cost-effective alternative for the production of dengue virus antigens. In this work, two ... Plant-based systems can be a safe and cost-effective alternative for the production of dengue virus antigens. In this work, two ... The generated plant made NS1, even without extensive purification, showed potential to be used for the development of the NS1 ... Interestingly, it was found that transient production of NS1-ER and NS1 ELP-ER using vacuum infiltration of whole plants, which ...
https://lib.ugent.be/catalog/pug01:188209
Bacterial and plant-produced scFv proteins have similar antigen-binding properties Anne-Marie Bruyns, Geert De Jaeger, Myriam ... Bacterial and plant-produced scFv proteins have similar antigen-binding properties. ISSN:. 0014-5793. Author:. Bruyns, Anne- ... "Bacterial and Plant-produced scFv Proteins Have Similar Antigen-binding Properties." FEBS LETTERS 386.1 (1996): 5-10. Print. ... "Bacterial and Plant-produced scFv Proteins Have Similar Antigen-binding Properties." Febs Letters 386 (1): 5-10. ...
Tandem fusion of hepatitis B core antigen allows assembly of virus-like particles in bacteria and plants with enhanced capacity...
Tandem fusion of hepatitis B core antigen allows assembly of virus-like particles in bacteria and plants with enhanced capacity ... Tandem fusion of hepatitis B core antigen allows assembly of virus-like particles in bacteria and plants with enhanced capacity ... We present the use of tandem core technology in both plant and bacterial expression systems. The results show that tandem core ... retains the ability to bind to its cognate antigen. This technology paves the way for the display of natively folded proteins ...
PCF1 and PCF2 specifically bind to cis elements in the rice proliferating cell nuclear antigen gene. | Plant Cell
Your Name) has sent you a message from Plant Cell Message Body (Your Name) thought you would like to see the Plant Cell web ... PCF1 and PCF2 specifically bind to cis elements in the rice proliferating cell nuclear antigen gene.. S Kosugi, Y Ohashi ... The Plant Cell Sep 1997, 9 (9) 1607-1619; DOI: 10.1105/tpc.9.9.1607 ... The Plant Cell Sep 1997, 9 (9) 1607-1619; DOI: 10.1105/tpc.9.9.1607 ...
Phytochemical-rich medicinal plant extracts suppress bacterial antigens-induced inflammation in human tonsil epithelial cells ...
Results The herbal plant extracts (≤5 µg/mL) were not cytotoxic to HTonEpiC. The extracts exhibited a broad range of reduction ... This study evaluated anti-inflammatory activity of phytochemical-rich extracts prepared from 12 herbal plants using human ... bacterial antigens) for 4 h and then exposed to ethanol extracts (EE) or aqueous extracts (AE) for 20 h. The secretion of four ... There is a growing interest in discovering plant-based anti-inflammatory compounds as potential alternatives to conventional ...
Plant purified ER-TG2 and vac-TG2 as antigen for EC dia | Open-i
Plant purified ER-TG2 and vac-TG2 as antigen for EC diagnosis. (A) Recognition of ER-TG2 and vac-TG2 by three different ... Figure 4: Plant purified ER-TG2 and vac-TG2 as antigen for EC diagnosis. (A) Recognition of ER-TG2 and vac-TG2 by three ... Figure 4: Plant purified ER-TG2 and vac-TG2 as antigen for EC diagnosis. (A) Recognition of ER-TG2 and vac-TG2 by three ... Plant purified TG2 recognition was also assayed by Western Blot (Figure 4C) confirming that the full-length ER- and vac-TG2 ...
Pharmaceutical proteins in plants: A strategic genetic engineering approach for the production of tuberculosis antigens -...
Production of protective antigens in transgenic plants, or "pharming," is a promising emerging approach, and a zoonosis-like TB ... Pharmaceutical proteins in plants: A strategic genetic engineering approach for the production of tuberculosis antigens. ... component is aimed at assessing the production efficacy and stability of the TB antigens in different compartments of the plant ...
Gut Antigen Sampling and Host Defense | Science Signaling
Engineering γδT cells limits tonic signaling associated with chimeric antigen receptors | Science Signaling
Plant Biology. Chimeric plants-the best of both worlds. *. Working Life. Building bridges ... Engineering γδT cells limits tonic signaling associated with chimeric antigen receptors. *View ORCID ProfileJonathan Fisher1,2 ... Engineering γδT cells limits tonic signaling associated with chimeric antigen receptors. By Jonathan Fisher, Roshan Sharma, ... Engineering γδT cells limits tonic signaling associated with chimeric antigen receptors. By Jonathan Fisher, Roshan Sharma, ...
Binding of C-reactive protein to antigen-induced but not mitogen-induced T lymphoblasts | Science
Plant Pathology. Plant nutrient acquisition entices herbivore. *. Working Life. The universe in a classroom ... Binding of CRP thus serves as a marker for antigen-reactive (-reacted) as opposed to mitogen-reative (-reacted) T cells, ... Binding of C-reactive protein to antigen-induced but not mitogen-induced T lymphoblasts ... Binding of C-reactive protein to antigen-induced but not mitogen-induced T lymphoblasts ...
Rapid antigen tests for dengue virus serotypes and Zika virus in patient serum | Science Translational Medicine
Plant Pathology. Plant nutrient acquisition entices herbivore. *. Working Life. The universe in a classroom ... Bosch et al. developed monoclonal antibodies to detect viral nonstructural 1 (NS1) protein antigens specific to dengue and Zika ... A low-cost, equipment-free rapid antigen test distinguishes dengue virus serotypes and Zika virus in patient sera without ... A low-cost, equipment-free rapid antigen test distinguishes dengue virus serotypes and Zika virus in patient sera without ...
Two-Photon Imaging of Lymphocyte Motility and Antigen Response in Intact Lymph Node | Science
Plant Pathology. Plant nutrient acquisition entices herbivore. *. Working Life. The universe in a classroom ... Lymphocyte motility is vital for trafficking within lymphoid organs and for initiating contact with antigen-presenting cells. ... Two-Photon Imaging of Lymphocyte Motility and Antigen Response in Intact Lymph Node ... Two-Photon Imaging of Lymphocyte Motility and Antigen Response in Intact Lymph Node ...
Antibiotic-Antigen Conjugates Bring Immunotherapy to Deadly Bacteria
The PMB-antigen conjugates successfully recruited the antibodies found in human serum, giving an indication that they may be ... Antibiotic-Antigen Conjugates Bring Immunotherapy to Deadly Bacteria. July 9, 2018. 0 ... Several promising antibiotic-antigen conjugate leads have been developed by scientists based at Lehigh University. These ... The scientists then followed up on these efforts by assembling antibiotic-antigen conjugates against gram-negative bacteria. ...
Recent Articles | Chimeric Antigen Receptor And Immunology | The Scientist Magazine®
Proliferating cell nuclear antigen - Wikipedia
"Two E2F elements regulate the proliferating cell nuclear antigen promoter differently during leaf development". Plant Cell. 14 ... Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic ... PCNA was originally identified as an antigen that is expressed in the nuclei of cells during the DNA synthesis phase of the ... "Entrez Gene: PCNA proliferating cell nuclear antigen". Leonardi E, Girlando S, Serio G, Mauri FA, Perrone G, Scampini S, Dalla ...
Implications of sequencing the nematode Caenorhabditis elegans genome for plant nematology
... gut antigens, dauer larva biology, synteny cloning, genome sequencing, evolution of plant parasitism, the nervous system and ... Evolution of plant parasitism. Plant nematology will benefit from immediate investigation of the evolution of plant parasitism ... Implications of sequencing the nematode Caenorhabditis elegans genome for plant nematology.. The Plant Health Instructor. DOI: ... The C. elegans genome sequence will allow molecular plant nematologists to enumerate presence/absence of plant parasitism- ...
New viral vectors for the expression of antigens and antibodies in plants.
... ... Pectate lyase activity was detected from plant extracts, suggesting the cause of severe necrotic reaction in plants. ... Future development of plant virus expression vectors needs to focus on the most important economic hosts, namely cereals and ... Foxtail mosaic virus (FoMV) is a species of the group Potexvirus, which infects cereal plants. A new viral vector series named ...
Plant-Based Compounds can Treat Prostate Cancer
Prostate Specific Antigen [PSA]. PSA blood test is specific to prostate gland but not necessarily a cancer specific test but is ... The study published online in Precision Oncology demonstrates how the plant-based chemicals work together. The research team ... Compounds found in plants have anti-cancer properties * Turmeric, apple peel, and red grapes contain compounds that prevent the ... The team concluded that the plant-based chemicals blocked the uptake of a nutrient needed by prostate cancer cells with ...
Processes | Free Full-Text | Light-Induced Production of An Antibody Fragment and Malaria Vaccine Antigen from Chlamydomonas...
... and malaria vaccine antigen (Pfs25), produced in the chloroplast of C. reinhardtii. To achieve a higher production of ... Plant J. 2002, 30, 733-744. [Google Scholar] [CrossRef] [PubMed]. *Gregory, J.A.; Topol, A.B.; Doerner, D.Z.; Mayfield, S.P. ... Plant Biotechnol. J. 2010, 8, 719-733. [Google Scholar] [CrossRef] [PubMed]. *Tran, M.; Van, C.; Barrera, D.J.; Pettersson, P.L ... Plant Biotechnol. J. 2007, 5, 402-412. [Google Scholar] [CrossRef] [PubMed]. *Surzycki, R.; Greenham, K.; Kitayama, K.; Dibal, ...
A polysaccharide fraction from medicinal herb Prunella vulgaris downregulates the expression of herpes simplex virus antigen in...
Prunella vulgaris L. (Labiatae), a perennial plant commonly found in China and Europe, has long been used as a … ... The HSV antigen increased time-dependently in the infected cells, and PPV reduced its expression. The effective concentrations ... Prunella vulgaris L. (Labiatae), a perennial plant commonly found in China and Europe, has long been used as a folk medicine to ... The novelty of PPV is that it also reduces the antigen expression of acyclovir-resistant strain of HSV-1. After incubations ...
Tandem Fusion of Hepatitis B Core Antigen Allows Assembly of Virus-Like Particles in Bacteria and Plants with Enhanced Capacity...
Tandem Fusion of Hepatitis B Core Antigen Allows Assembly of Virus-Like Particles in Bacteria and Plants with Enhanced Capacity ... Tandem Fusion of Hepatitis B Core Antigen Allows Assembly of Virus-Like Particles in Bacteria and Plants with Enhanced Capacity ... Tandem Fusion of Hepatitis B Core Antigen Allows Assembly of Virus-Like Particles in Bacteria and Plants with Enhanced Capacity ...
Chmadrin: a novel Ki-67 antigen-related perichromosomal protein possibly implicated in higher order chromatin structure |...
Special issue on plant cell biology. Weve just published our special issue focussing on plant cell biology - check out the ... Building a plant cell wall. In their Cell Science at a Glance article and accompanying poster, Staffan Persson and colleagues ... Chasing Calcium in plant peroxisomes. In their research article, Francisco Corpas and Juan Barroso reveal the importance of ... In this interview, our Special Issues guest editor Jenny Russinova discusses her excitement for plant cell biology and what ...
Hla Antigens | Encyclopedia.com
The human leukocyte antigen (HLA) is not a single antigen, but is rather a group of proteins that are located on the surface of ... antigen A Dictionary of Plant Sciences © A Dictionary of Plant Sciences 1998, originally published by Oxford University Press ... Antigen World of Forensic Science COPYRIGHT 2005 Thomson Gale. Antigen. Antigens, which are usually proteins or polysaccharides ... Human leukocyte antigen (HLA). The human leukocyte antigen (HLA) is not a single antigen, but is rather a group of proteins ...
AntibodyCore antigenProteinsSurface antigenVaccine antigensArabidopsisMonoclonal antibodiesCalled Rapid antigen testsHepatitisChimericBacterialReceptorRice prolifeViralForeign antigensImmunologyImmunogenicDengue virusTumorSynthesisSolubleGenesNicotianaVaccinesGram-negativeExpressionEpitopesBacteriaPurificationMoleculeVitroPCNAVirusesGeneTransgenic plantPollenImmuneProductionHBsAgTherapeuticUrineSubstancesPolysaccharideTobaccoDiagnosisExtractsRapidlyCompositionStimulationCellFungiChemicalELISAGlycoprotein
Antibody25
- Higher monoclonal antibody binding of 67.87% of the antigen was observed when it was expressed with a C-terminal ER retention signal. (springer.com)
- Further analysis revealed that plant-produced DIII was processed properly and demonstrated specific binding to an anti-DIII monoclonal antibody that recognizes a conformational epitope. (hindawi.com)
- After oral intake of fresh transgenic plant material, a proportionof the mice elicited specific IgG antibody responses, as compared to the control animals that were fedwild-type plants and of which none sero-converted. (diva-portal.org)
- A gene encoding a single-chain variable (scFv) antibody fragment was expressed as a cytoplasmic and endoplasmic reticulum-targeted protein in transgenic tobacco plants, In both cases, the scFv accu. (ugent.be)
- Antibodies against proliferating cell nuclear antigen (PCNA) or monoclonal antibody termed Ki-67 can be used for grading of different neoplasms, e.g. astrocytoma. (wikipedia.org)
- The possibility of producing complex and diverse therapeutic proteins, such as monoclonal antibodies, antibody conjugates and vaccine antigens, in the chloroplast of Chlamydomonas reinhardtii has been reported [ 1 , 2 , 3 , 4 , 5 , 6 ]. (mdpi.com)
- in 1951 and is named after a patient (Mrs Kidd delivered a baby with a haemolytic disease of the newborn associated with an antibody directed against a new antigen Jk (a). (wikipedia.org)
- Although T cells are presumed to be the critical mediators of graft-versus-leukemia, our previous studies have shown that DLI initiates a complex immune response that includes a potent antibody response to a variety of leukemia-associated antigens ( 16 ). (pnas.org)
- Using established methods for serological identification of tumor antigens by recombinant cDNA expression cloning (SEREX) ( 17 , 18 ) we identified a panel of 13 leukemia-associated antigens that were recognized by high titer antibody 1 year after response to DLI. (pnas.org)
- The cellular basis of immunity and the mechanisms of antigen-antibody interactions are included. (ets.org)
- Immunohistochemistry: Lewis A Blood Group Antigen Antibody (7LE) [NB500-525] - Staining of human colon adenocarcinoma (paraffin-embedded sections) with anti-Blood Group Lewis a (7LE). (novusbio.com)
- The antibody 7LE recognizes Lewisa blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, expressed in colonic epithelial cells. (novusbio.com)
- Discover related pathways, diseases and genes to Lewis A Blood Group Antigen Antibody (NB500-525). (novusbio.com)
- Actin1 Monoclonal antibody specifically detects Actin1 in Plant samples. (fishersci.com)
- This mouse monoclonal antibody was generated against plant actin ACT8 and recognizes all tested subclasses of plant actins (arabidopsis ACT1, 2, 3, 4, 7, 8, 11, 12) and Dictyosetelium actin. (fishersci.com)
- Transgenic plants expressing a functional single-chain Fv antibody are specifically protected from virus attack," Nature, vol. 366, pp. 469-472, Dec. 2, 1993. (freepatentsonline.com)
- The C-terminal KDEL sequence increases the expression level of a single-chain antibody designed to be targeted to both the cytosol and the secretory pathway in transgenic tobacco," Plant Molecular Biology, vol. 30, pp. 781-793, 1996. (freepatentsonline.com)
- Characterization of recombinant scFv antibody reactive with an apical antigen of Eimeria acervulina," Biotechnology Letters, vol. 23, No. 12, pp. 949-955, Jun. (freepatentsonline.com)
- Fusion proteins comprising a Fusarium-specific antibody linked to antifungal peptides protect plants against a fungal pathogen", Nature Biotechnology, 22(6):Jun. (freepatentsonline.com)
- Each antibody is crafted with care according to rigorous protocols for immunogen design and preparation, presentation to host animal, and high-affinity purification against the antigen. (abgent.com)
- The gels are blotted onto nitrocellulose and treated with a monoclonal antibody against SV40 T antigen or against the tumor suppressor p53. (nottingham.ac.uk)
- Antibody made in response to a foreign red cell antigen is usually not harmful but does require subsequent transfusions to be antigen-negative. (britannica.com)
- Monoclonal Anti-Actin (plant) antibody recognizes actin in several plant species including Dictyostelium . (sigmaaldrich.com)
- Monoclonal Anti-Actin (plant) antibody is suitable for use in western blot (1-2 μg/mL using corn leaves extracts), indirect ELISA and immunohistochemistry. (sigmaaldrich.com)
- The next time the same antigen contacts the body, a full-scale immune response is not needed as the body already has a specific antibody available instantly for that antigen. (wikipedia.org)
Core antigen4
- Tandem fusion of hepatitis B core antigen allows assembly of virus-like particles in bacteria and plants with enhanced capacity to accommodate foreign proteins. (jic.ac.uk)
- Several vaccine antigens, including Norwalk virus capsid protein and hepatitis B core antigen, were expressed using the BeYDV vector at levels up to 1 mg per g of leaf mass. (elsevier.com)
- Hepatitis B core antigen (HBcAg) is an immunogenic protein that assembles into 30 or 34 nm diameter VLPs. (worktribe.com)
- We have constructed a recombinant viral vector based on potato X virus that provides for expression in the Nicotiana benthamiana plants of a hybrid protein M2eHBc consisting of an extracellular domain of influenza virus M2 protein (M2e) fused to hepatitis B core antigen (HBc). (chemweb.com)
Proteins16
- Cramer CL, Boothe JG, Oishi KK (1999) Transgenic plants for therapeutic proteins: linking upstream and down stream strategies. (springer.com)
- Expression systems based on plants may provide solutions to overcome these challenges, because they provide highly scalable production of recombinant proteins at low cost and have a low risk of introducing adventitious human or animal viruses or prions [ 11 , 12 ]. (hindawi.com)
- The availability of recombinant N and M proteins from plants opens the way to further evaluation of their potential utility for the development of diagnostic and protection/therapy tools to be quickly manufactured, at low cost and with minimal risk, to face potential new highly infectious SARS-CoV outbreaks. (frontiersin.org)
- Interestingly, it was found that transient production of NS1-ER and NS1 ELP-ER using vacuum infiltration of whole plants, which is easier to scale up, rather than syringe infiltration of leaves, greatly improved the accumulation of NS1 proteins. (frontiersin.org)
- Bruyns A-M, De Jaeger G, De Neve M, De Wilde C, Van Montagu M, Depicker A. Bacterial and plant-produced scFv proteins have similar antigen-binding properties. (ugent.be)
- Both inserted proteins are correctly folded and the nanobody fused to the surface of the tandem core particle (which we name tandibody) retains the ability to bind to its cognate antigen. (jic.ac.uk)
- These results confirmed the usefulness of plant-produced TG2 to develop screening assays.In conclusion, the combination of subcellular sorting strategy with co-expression with a PB inducing construct was sufficient to increase TG2 protein yields.This type of approach could be extended to other problematic proteins, highlighting the advantages of plant based production platforms. (nih.gov)
- This type of approach could be extended to other problematic proteins, highlighting the advantages of plant based production platforms. (nih.gov)
- The human leukocyte antigen (HLA) is not a single antigen, but is rather a group of proteins that are located on the surface of white blood cells. (encyclopedia.com)
- Research on human blood cells in the 1950s identified three genes associated with the HLA (HLA-A, HLA-B, HLA-C). In the 1970s, another gene was identified (HLA-D). With the advent of molecular technology beginning in the 1980s, more genes that code for proteins that function in the antigen complex have continued to be identified. (encyclopedia.com)
- The Kidd antigen system (also known as Jk antigen) are proteins found in the Kidd's blood group, which act as antigens, i.e., they have the ability to produce antibodies under certain circumstances. (wikipedia.org)
- Biological aerosols carrying antigenic proteins, such as pollen grains or plant-derived paucimicronic components, can produce allergic symptoms. (ersjournals.com)
- Geminiviruses such as bean yellow dwarf virus (BeYDV) have a small, single stranded DNA genome that replicates in the nucleus of an infected plant cell, using the cellular DNA synthesis apparatus and a virus-encoded replication initiator protein (Rep). BeYDV-derived expression vectors contain deletions of the viral genes encoding coat and movement proteins and insertion of an expression cassette for a protein of interest. (elsevier.com)
- This spike fragment resembles the bacterial toxins known as super antigens-proteins that generate excessive reaction from T cells, a vital member of the immune system. (nationalgeographic.com)
- GMO examples: potato plants encoded with immunogenic proteins of Newcastle Disease Virus. (greenmedinfo.com)
- Serum precipitins to a standard commercial antigen panel (including bacteria, fungi, and avian proteins that have been associated with HP in other settings) were negative in the two workers who were tested. (cdc.gov)
Surface antigen5
- Rasthali (AAB) have been transformed with the ' s ' gene of hepatitis B surface antigen (HBsAg) using Agrobacterium mediated transformation. (springer.com)
- cDNA sequence analysis of a 29-kDa cysteine-rich surface antigen of pathogenic Entamoeba histolytica. (pnas.org)
- A 29-kDa protein was identified as a surface antigen when mAbs were used to immunoprecipitate the antigen from metabolically 35S-labeled live trophozoites. (pnas.org)
- The O antigen is a major surface antigen present in gram-negative bacteria, and it is responsible for serological diversity. (asm.org)
- A potato has been engineered which expresses hepatitis B surface antigen. (greenmedinfo.com)
Vaccine antigens1
- Expression of recombinant vaccine antigens and monoclonal antibodies using plant viral vectors has developed extensively during the past several years. (elsevier.com)
Arabidopsis6
- Here, we report the expression in transformed plants (Arabidopsis thaliana) of Rift Valley fever virusantigens. (diva-portal.org)
- Brassicaceae) tissue cultures, recognizes an antigen in the Arabidopsis ( Arabidopsis thaliana ) ecotype Columbia that is associated specifically with the plasma membrane of sieve elements, but not companion cells, and accumulates at the earliest stages of sieve element differentiation. (plantphysiol.org)
- The identity of the RS6 antigen was revealed by reverse transcription-PCR of Arabidopsis leaf RNA using degenerate primers to be an early nodulin (ENOD)-like protein that is encoded by the expressed gene At3g20570 . (plantphysiol.org)
- Recognizes subclasses 4 and 5 of vegetative plant actins (arabidopsis ACT1, 3, 4, 12). (fishersci.com)
- AtPex16p in peroxisomes of Arabidopsis plants. (plantphysiol.org)
- The goal of this laboratory exercise is to provide a laboratory experience for undergraduates, in which they apply fundamental genetic principles to the study of a complex developmental process, specifically, root cell shape determination in the simple plant Arabidopsis thaliana. (nottingham.ac.uk)
Monoclonal antibodies2
- Plant purified ER-TG2 and vac-TG2 were recognized by three anti-TG2 monoclonal antibodies that bind different epitopes proving that plant-produced antigen has immunochemical characteristics similar to those of human TG2. (nih.gov)
- In addition to their use in blood grouping, these monoclonal antibodies can be of use in defining the hereditary background (heterogenicity) and structure of the red cell antigen . (britannica.com)
Called Rapid antigen tests1
- These are called Rapid antigen tests (RATs). (news-medical.net)
Hepatitis2
- A carrot has been engineered to express a Hepatitis B antigen. (greenmedinfo.com)
- An edible hepatitis B antigen vaccine has been developed for maize. (greenmedinfo.com)
Chimeric1
- Adoptive transfer of T cells engineered to express a chimeric antigen receptor (CAR) is an effective therapy for select lymphomas. (sciencemag.org)
Bacterial4
- We present the use of tandem core technology in both plant and bacterial expression systems. (jic.ac.uk)
- bacterial antigens) for 4 h and then exposed to ethanol extracts (EE) or aqueous extracts (AE) for 20 h. (peerj.com)
- 1998). Other comparisons of bacterial, yeast, nematode, plant, mouse and human genomes will reveal unique and surprising aspects of the genetic make-up of organisms. (apsnet.org)
- Several of the identified T-cell epitopes share similarity with common bacterial and viral antigens, suggesting the involvement of pre-existing microbial cross-reactive T cells in rapid and durable tumour regression seen in some patients. (bireme.br)
Receptor3
- The role that K antigen plays in sensitivity to this phage is unknown, but in a simple model the K antigen would act as a ligand for a receptor on the phage particle. (asm.org)
- This study shows that human and mouse T lymphocytes express AMPKα1 and that this is rapidly activated in response to triggering of the T cell antigen receptor (TCR). (rupress.org)
- The development of immunoglobulin receptor repertoire sequencing methods makes it possible to perform repertoire-wide disease association studies of antigen receptor sequences. (elifesciences.org)
Rice prolife3
- The recombinant rice XRCC1 (OsXRCC1) protein binds single-stranded DNA (ssDNA) as well as double-stranded DNA (dsDNA) and also interacts with rice proliferating cell nuclear antigen (OsPCNA) in a pull-down assay. (nih.gov)
- PCF1 and PCF2 specifically bind to cis elements in the rice proliferating cell nuclear antigen gene. (plantcell.org)
- We have previously defined the promoter elements, sites IIa and IIb, in the rice proliferating cell nuclear antigen (PCNA) gene that are essential for meristematic tissue-specific expression. (plantcell.org)
Viral2
- New viral vectors for the expression of antigens and antibodies in plants. (tdl.org)
- This vector was introduced into plant cells by infiltrating leaves with agrobacteria carrying the viral vector. (chemweb.com)
Foreign antigens2
- Knowing the "self" antigen allows the immune system to rapidly distinguish foreign antigens. (encyclopedia.com)
- The two classes of histocompatibility molecules allow an organism to in essence establish an inventory of what cells are "self" and to expose foreign antigens to the immune system so that antibodies to these antigens can be made. (encyclopedia.com)
Immunology2
- In: Hammond J, Mc Garvey P, Yusibov V (eds) Current topics in microbiology and immunology, plant biotechnology: new products and applications, vol 240. (springer.com)
- In this project we have put together partners covering methods of antigen production in plants (tobacco plants, Bioforsk/Max Planck Institute), a leading research group in nanoparticle formulation (Langer ́s group at MIT, USA), and two fish immunology/vaccinology groups (NMBU/UIT). (uit.no)
Immunogenic3
- This study provided the proof of principle for rapidly producing immunogenic vaccine candidates against WNV in plants with low cost and scalability. (hindawi.com)
- Thus, we show that transgenic plants can be readily used to express and produce Rift Valley Fever virusproteins, and that the plants are immunogenic when given orally to mice. (diva-portal.org)
- These findings suggest that CML66 may be immunogenic in a wide variety of malignancies and may be a target for antigen-specific immunotherapy. (pnas.org)
Dengue virus2
- The implementation of extensive screening tests is indispensable to control this disease, and the Dengue virus non-structural protein 1 (NS1) is a promising antigen for the serological diagnosis of dengue fever. (frontiersin.org)
- Plant-based systems can be a safe and cost-effective alternative for the production of dengue virus antigens. (frontiersin.org)
Tumor5
- This report describes a tumor-associated antigen, termed CML66, initially cloned from a chronic myelogenous leukemia (CML) cDNA expression library. (pnas.org)
- On immunohistochemical study, tumor cells exhibited characteristics of mesenchymal and endothelial origin, i.e., strong to moderate immune reactivity against vimentin, factor VIII-related antigen (F8RA), Ulex europaeus agglutinin type 1 lectin (UEA-1), and CD 34, but not against keratin, epithelial membrane antigen (EMA) or S-100 protein (S100). (biomedsearch.com)
- aims to bring together leading academic scientists, researchers and research scholars to exchange and share their experiences and research results on all aspects of Antigens, Tumor-Associated Antigens and Cancer Therapy. (waset.org)
- Also, high quality research contributions describing original and unpublished results of conceptual, constructive, empirical, experimental, or theoretical work in all areas of Antigens, Tumor-Associated Antigens and Cancer Therapy are cordially invited for presentation at the conference. (waset.org)
- ICATAACT 2022 has teamed up with the Special Journal Issue on Antigens, Tumor-Associated Antigens and Cancer Therapy . (waset.org)
Synthesis8
- PCNA was originally identified as an antigen that is expressed in the nuclei of cells during the DNA synthesis phase of the cell cycle. (wikipedia.org)
- Previously isolated mutants defective in the synthesis of K antigen are resistant to bacteriophage φ16-3. (asm.org)
- By screening ca. 100,000 Tn 5 -mutagenized R. meliloti bacteria for resistance to bacteriophage φ16-3, we isolated 119 mutants, 31 of which could not be complemented by genes previously identified as being required for K-antigen synthesis. (asm.org)
- Although all of these mutants lack the K antigen, many also have altered lipopolysaccharides (LPS), suggesting that the biochemical pathways for the synthesis of K antigen and LPS have common enzymatic steps. (asm.org)
- Our studies have identified new classes of genes required for both the synthesis of K antigen and the symbiotic proficiency of S. meliloti AK631. (asm.org)
- We therefore reasoned that resistance to phage φ16-3 could be used as a powerful selection factor in isolating additional mutations in genes required for the synthesis of K antigen. (asm.org)
- Screening for mutations in genes involved in K-antigen synthesis by selecting for mutants with resistance to phage φ16-3. (asm.org)
- Genes involved in O-antigen synthesis and downstream assembly map to the rfb locus located between the galF and gnd genes in many Enterobacteriaceae . (asm.org)
Soluble3
- The hybrid protein M2eHBc was synthesized in the infected N. benthamiana plants in an amount reaching 1-2% of the total soluble protein and formed virus-like particles with the M2e peptide presented on the surface. (chemweb.com)
- 7. The composition of claim 1 further comprising a soluble antigen. (google.com)
- His symptoms were temporally related to working in an area of the plant where soluble machining oils -- a type of MWF -- were used. (cdc.gov)
Genes5
- In plants, there are no DNA polymerase beta (Pol beta) and DNA ligase III (Lig3) genes. (nih.gov)
- However, the fact that none of these genes were predicted to be glycosyltransferases, as have been found in the succinoglycan ( 5 , 6 , 14 ) and EPS II ( 1 ) biosynthetic regions, suggested that there must be additional genetic loci that encode functions required for K-antigen biosynthesis. (asm.org)
- Within this panel of antigens, 11 represented known genes and two represented genes that had not previously been identified. (pnas.org)
- Such transgenic plants will express alien genes, virtually from any organism, provided the genes are flanked by appropriate controlling elements. (whsmith.co.uk)
- About one-half of the 23 predicted plant PEX genes have been shown via subcellular localizations and/or reverse genetics experiments to code for actual peroxin homologs of yeast or mammalian peroxins. (plantphysiol.org)
Nicotiana2
- GH human Recombinant produced in Nicotiana benthamiana plant is a single chain containing 205 amino acids (molecular formula C 1025 H 1570 N 280 O 306 S 7 ) and 6-His-tag at the N-terminal having the total molecular mass of 22.9kDa. (prospecbio.com)
- In this work, a transient expression system for the production of human TG2 in Nicotiana benthamiana leaves was optimized and reactivity of plant-produced TG2 in CD screening test was evaluated. (nih.gov)
Vaccines4
- Indeed, virus-specific and 'orphan' vaccine candidates and therapeutics represent one of the most interesting applications of the plant-based technology, especially when it is necessary to produce 'rapid response' vaccines such as those directed against bioterrorism agents and diseases with pandemic potential, like influenza. (frontiersin.org)
- These are promising findingsand provide a basis for further studies on edible plant vaccines against the Rift Valley fever virus. (diva-portal.org)
- Methods and compositions are provided for the encapsulation of antigens in PLGA microspheres for use as vaccines. (google.com)
- This invention relates to the microencapsulation of antigens for use as therapeutic or prophylactic vaccines. (google.com)
Gram-negative1
- The scientists then followed up on these efforts by assembling antibiotic-antigen conjugates against gram-negative bacteria. (genengnews.com)
Expression16
- The transgenic nature of the plants and expression of the antigen was confirmed by PCR, Southern hybridization and reverse transcription (RT)-PCR. (springer.com)
- The expression levels of the antigen in the plants grown under in vitro conditions as well as the green house hardened plants were estimated by ELISA for all the four constructs. (springer.com)
- Maximum expression level of 38 ng/g F.W. of leaves was noted in plants transformed with pEFEHBS grown under in vitro conditions, whereas pHER transformed plants grown in the green house showed the maximum expression level of 19.92 ng/g F.W. of leaves. (springer.com)
- The transgenic plants were grown up to maturity in the green house and the expression of HBsAg in the fruits was confirmed by RT-PCR. (springer.com)
- Attempts were also made to enhance the expression of HBsAg in the leaves of transgenic banana plants by wounding and/or treatment with plant growth regulators. (springer.com)
- The NS1 fused to ELP and targeted to the ER (NS1 ELP-ER) showed the highest yield (445 mg/kg), approximately a forty-fold increase in accumulation levels compared to the non-fused protein (NS1-ER), representing the first example of transient expression of DENV NS1 in plant. (frontiersin.org)
- Plant-based transient expression systems can be an alternative for the production of this protein. (nih.gov)
- Plants viruses are increasingly being examined as alternative recombinant protein expression systems. (tdl.org)
- Future development of plant virus expression vectors needs to focus on the most important economic hosts, namely cereals and legumes, to develop tools to aid breeding of such hosts and systems for edible vaccine production. (tdl.org)
- This may prove a useful and environmentally safe vector for both transient expression and perhaps transgenic plants. (tdl.org)
- The HSV antigen increased time-dependently in the infected cells, and PPV reduced its expression. (nih.gov)
- The novelty of PPV is that it also reduces the antigen expression of acyclovir-resistant strain of HSV-1. (nih.gov)
- Expression of Engineered Antibodies in Plants: A Possible Tool for Spiroplasma and Phytoplasma Disease Control," Phytopathology, vol. 88, No. 12, pp. 1367-1371, Dec. 1998. (freepatentsonline.com)
- 2. The polynucleotide of claim 1, further comprising a regulatory sequence for expression in a plant, plant organ, plant tissue, or plant cell. (freepatentsonline.com)
- His research interests include the genetics and physiology of sex expression in plants through developmental genetics, somatic hybridization in plants, production of plant cybrids and molecular genetics of stress resistance. (whsmith.co.uk)
- Tobacco expression system will produce surface antigens of piscine reovirus that will be delivered as a nanoparticle-based vaccine to fish. (uit.no)
Epitopes3
- To test their usefulness as antigen their recognition by the mAbs 2G3, 5G7, or 4E1, which recognize different TG2 epitopes, was analyzed by immunoblot. (nih.gov)
- Both vac-TG2 and ER-TG2 were positively recognized by these antibodies as is shown Figure 4A, confirming that although in humans TG2 is a localized in the cytosol, the introduction into the plant secretory pathway do not affect the structure of the epitopes recognized by these mAbs. (nih.gov)
- mAbs recognized epitopes on the 29-kDa native antigen on some but not all clinical isolates tested. (pnas.org)
Bacteria7
- The PMB-antigen conjugates successfully recruited the antibodies found in human serum, giving an indication that they may be capable of harnessing the immune system to eliminate dangerous, disease-causing bacteria. (genengnews.com)
- Plant-parasitic nematodes are obligate parasites that cannot be grown on plates of bacteria as a food source to provide large numbers of individuals as can certain free-living nematodes (e.g. (apsnet.org)
- Plant Pathogenic Bacteria, 66, 331-335. (gva.es)
- In contrast, S. meliloti mutants that fail to synthesize at least one of these polysaccharides in symbiotically appropriate forms will produce ineffective or Fix − nodules, which contain few bacteria and which are incapable of fixing nitrogen on alfalfa host plants. (asm.org)
- An antigen is a molecule (usually a protein ) expressed by a bacteria or virus that is recognized by the adaptive immune system as foreign which can stimulate the production of antibodies and combine specifically with them. (wikipedia.org)
- Antigens include bacteria , cells of transplanted organs , plant pollen and toxins . (wikipedia.org)
- Vaccinations usually contain dead bacteria or antigen so the antibodies can familiarise themselves and kill it. (wikipedia.org)
Purification1
- The generated plant made NS1, even without extensive purification, showed potential to be used for the development of the NS1 diagnostic tests in resource-limited areas where dengue is endemic. (frontiersin.org)
Molecule2
- At the outer surface of the cell the molecule contains an antigen that has been acquired from the surrounding environment. (encyclopedia.com)
- Usually an antigen is a molecule , perhaps on the cell surface of a bacterium or virus . (wikipedia.org)
Vitro2
- These transgenic plants were multiplied under in vitro using floral apex cultures. (springer.com)
- This study evaluated anti-inflammatory activity of phytochemical-rich extracts prepared from 12 herbal plants using human tonsil epithelial cells (HTonEpiC) in vitro . (peerj.com)
PCNA1
- Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. (wikipedia.org)
Viruses1
- Plants are locked in an ancient arms race with hostile viruses, but genome editing is giving crops the upper hand. (the-scientist.com)
Gene8
- The authors thank Dr. G.D. May, Boyce Thompson Institute for Plant research Inc., Ithaca, NY, USA, for providing the EFE gene promoter of banana. (springer.com)
- Hood EE, Gelvin SB, Melchers LS, Hoekama A (1993) New Agrobacterium helper plasmid for gene transfer to plant cells. (springer.com)
- So far, two gene regions that are required for the production of K antigen have been identified. (asm.org)
- Using this method, we carried out a large-scale genetic study that resulted in the identification of four new, phenotypically and genetically distinct classes of K-antigen mutations which are not complemented by plasmids carrying the rkp-1 region or the rkpZ + gene. (asm.org)
- Kidd antigens are enhanced by enzymes Jk(a-b-) represents the null phenotype and usually results from homozygosity for a silent gene at the JK locus. (wikipedia.org)
- Characterization of a wheat class Ib chitinase gene differentially induced in isogenic lines by infection with Puccinia graminis," Plant Science, vol. 103, No. 2, pp. 177-187, 1994. (freepatentsonline.com)
- O-antigen serotypes emerge as a consequence of the gene content rather than sequence variation at this locus ( 44 ). (asm.org)
- The O-antigen gene cluster of E. sakazakii has not been previously characterized. (asm.org)
Transgenic plant1
- A transgenic plant expressing the glycoprotein of Crimean-Congo hemorrhagic fever virus has been developed and tested in mice as an oral immunogen. (greenmedinfo.com)
Pollen1
- Allergens, like pollen, are nothing more than foreign plant antigens. (medlineplus.gov)
Immune7
- Essentially the different HLA arrangement on cells allows the immune system to develop an inventory of "self" antigens in the body. (encyclopedia.com)
- Rather, class II molecules are on the surface of immune cells such as macrophages and B-lymphocytes that are designed to process cells and present the antigens from these cells to T lymphocytes. (encyclopedia.com)
- Defects in the structure of the HLAs is the cause of some diseases where the body's immune system perceives a host antigen as foreign and begins to attack the body's own tissue. (encyclopedia.com)
- Innate immune responses are rapid and independent of antigen. (nature.com)
- Innate immune systems are found in all classes of plants and animals. (nature.com)
- Diverse repertoires of hypervariable immunoglobulin receptors (TCR and BCR) recognize antigens in the adaptive immune system. (elifesciences.org)
- The first time that a new antigen comes into contact with the body the response of the immune system will be a complete immune response . (wikipedia.org)
Production10
- We described the rapid production of the domain III (DIII) of the envelope (E) protein in plants as a vaccine candidate for West Nile Virus (WNV). (hindawi.com)
- For the M protein, this is the first description of production in plants, while for plant-derived N protein we demonstrate that it is recognized by sera of patients from the SARS outbreak in Hong Kong in 2003. (frontiersin.org)
- Production of protective antigens in transgenic plants, or "pharming," is a promising emerging approach, and a zoonosis-like TB is a good model for investigating the potential of this approach. (cirad.fr)
- Pharma-Planta, a European Commission-funded project and consortium, was set up to address this topic, within which a component is aimed at assessing the production efficacy and stability of the TB antigens in different compartments of the plant cell. (cirad.fr)
- 6-Benzylaminopurine (6-BA), a cytokinin plant growth regulator, has been banned for use in bean sprout production in China. (cdc.gov)
- The 128,000 square-foot facility will be the company's highest-volume production plant in the world and begins operations in the second half of 2021 with a mission to produce 600 million QuickVue SARS rapid antigen tests per year for the detection and diagnosis of COVID-19 infections. (massdevice.com)
- This book provides the molecular and biotechnological background for genetic transformation in plants, as well as updated information about the production of antibodies, antigens and other medical and health products by transgenic plants. (whsmith.co.uk)
- In recent years, Prof Galun is engaged in the production of transgenic plants. (whsmith.co.uk)
- When an antigen is introduced into the body it causes the production of antibodies. (wikipedia.org)
- Antigens stimulate the production of antibodies: they do not produce them directly. (wikipedia.org)
HBsAg1
- HBsAg obtained from transgenic banana plants is similar to human serum derived one in buoyant density properties. (springer.com)
Therapeutic1
- In the last decade, studies have highlighted the potential therapeutic compounds found in plants, including chemical in foods such as turmeric, apple peels, red grapes and green tea. (medindia.net)
Urine2
- in 1953, individuals who lack the Jk antigen (Jk null) are unable to maximally concentrate their urine. (wikipedia.org)
- all four diagnoses were confirmed by Legionella urine antigen. (cdc.gov)
Substances1
- Additional substances with specific blood group activity have been found in certain plants. (britannica.com)
Polysaccharide4
- In this study, a polysaccharide fraction was prepared from Prunella vulgaris (PPV), and its effects on the expressions of HSV-1 and HSV-2 antigens in their host Vero cells were investigated with flow cytometry. (nih.gov)
- After incubations with 25-100 microg/ml of PPV the HSV antigen-positive cells were reduced by 24.8-92.6%, respectively, showing that this polysaccharide fraction has a different mode of anti-HSV action from acyclovir. (nih.gov)
- For Sinorhizobium meliloti (also known as Rhizobium meliloti ) AK631 to establish effective symbiosis with alfalfa, it must be able to synthesize a symbiotically active form of its K antigen, a capsular polysaccharide containing a Kdo (3-deoxy- d -manno-octulosonic acid) derivative. (asm.org)
- This is composed of three parts, a complex lipid, called lipid A (consisting of sugars and fatty acids), that anchors the structure to the outer membrane, a conserved core oligosaccharide, and variable polysaccharide side chains (O antigen) that extend from the latter core. (asm.org)
Tobacco1
- Removal of Vascuolar Targeting Signal from Class I Vasuolar Chitinase leads to its Extracellular Secretion in Transgenic Tobacco," Journal of Plant Biochemistry and Biotechnology, vol. 10, No. 2, pp. 139-142, Jul. (freepatentsonline.com)
Diagnosis1
- Plant purified ER-TG2 and vac-TG2 as antigen for EC diagnosis. (nih.gov)
Extracts2
Rapidly1
- The FDA issued an emergency use authorization today for the Quidel Corp. (NSDW:QDEL) QuickVue At-Home COVID-19 test, another antigen test to rapidly collect and test samples at home, without the need to send a sample to a laboratory for analysis. (massdevice.com)
Composition2
- The O-antigen locus varies in size for each serotype depending on the sugar composition and complexity of the antigen structure ( 34 ). (asm.org)
- 5. The composition of claim 3 wherein the adjuvant is coencapsulated with the antigen in the microspheres of said homogeneous population. (google.com)
Stimulation1
- The C-reactive protein (CRP), an acute phase reactant which binds selectively to T (thymus-derived) lymphocytes, was found to bind to lymphoblasts formed upon stimulation with antigens but not with mitogens. (sciencemag.org)
Cell11
- The plant XRCC1 lacks the N-terminal domain (NTD) which is required for Pol beta binding and is essential for mammalian cell survival. (nih.gov)
- Thank you for your interest in spreading the word on Plant Cell. (plantcell.org)
- Message Body (Your Name) thought you would like to see the Plant Cell web site. (plantcell.org)
- The potency of this therapy can be limited by antigen-independent (tonic) signaling, which promotes progressive CAR-T cell inactivation. (sciencemag.org)
- We've just published our special issue focussing on plant cell biology - check out the table of contents here , and a selection of the issue's highlights below. (biologists.org)
- In this interview , our Special Issue's guest editor Jenny Russinova discusses her excitement for plant cell biology and what she hopes to achieve with the issue. (biologists.org)
- Thomas Ott and colleagues review the challenges and opportunities of quantitative live-cell imaging in plants. (biologists.org)
- Kidd antigens are located on a red blood cell urea transporter (human urea transporter 11- HUT11 or UT-B1). (wikipedia.org)
- In addition, women who hit the allergy trifecta of sensitivity to plants, grass, and trees had a 73% greater chance of developing a mature B-cell lymphoma or related disorder. (medscape.com)
- for example, group A blood contains A antigens on red cell surfaces and anti-B antibodies in the surrounding serum. (britannica.com)
- Immunofluorescence microscopy with antigen affinity-purified IgGs showed an unambiguous, steady-state coexistence of AtPex16p in suspension cell peroxisomes and ER. (plantphysiol.org)
Fungi1
- 1. (Plants) any member of a kingdom of organisms (Fungi) that lack chlorophyll, leaves, true stems, and roots, reproduce by spores, and live as saprotrophs or parasites. (thefreedictionary.com)
Chemical1
- Because the HLA is a chemical tag that distinguishes "self" from "nonself," the antigen is important in the rejection of transplanted tissue and in the development of certain diseases (e.g., insulin-dependent diabetes). (encyclopedia.com)
ELISA1
- In order to test the performance of the plant purified ER-TG2 and vac-TG2 version in CD screening test an ELISA was performed using a pool of 12 sera of CD patient and control healthy donors (Figure 4B). (nih.gov)
Glycoprotein2
- The antigens used in this study were the N protein and a deletion mutant of the Gn glycoprotein.Transformed lines were analysed for specific mRNA and protein content by RT-PCR and Westernblotting, respectively. (diva-portal.org)
- Kidd comprises three antigens on a glycoprotein with 10 transmembrane spanning domains, cytoplasmic N- and C-termini and one extracellular N-glycosylation site. (wikipedia.org)