Antibodies, Heterophile
Antibodies elicited in a different species from which the antigen originated. These antibodies are directed against a wide variety of interspecies-specific antigens, the best known of which are Forssman, Hanganutziu-Deicher (H-D), and Paul-Bunnell (P-B). Incidence of antibodies to these antigens--i.e., the phenomenon of heterophile antibody response--is useful in the serodiagnosis, pathogenesis, and prognosis of infection and latent infectious states as well as in cancer classification.
Antigens, Heterophile
Infectious Mononucleosis
Forssman Antigen
Immunoconglutinins
Hemagglutination Tests
Latex Fixation Tests
False Positive Reactions
Immunoassay
Antigens, Neoplasm
Antigens, Surface
Herpesvirus 4, Human
Immunoglobulin M
Reagent Kits, Diagnostic
Immunoglobulin G
Antigens, Protozoan
Antigens, Polyomavirus Transforming
Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.
HLA Antigens
Epstein-Barr Virus Infections
Epstein-Barr Virus Nuclear Antigens
Induction of complement attack on human cells by Gal(alpha1,3)Gal xenoantigen expression as a gene therapy approach to cancer. (1/163)
Galactose(alpha1,3)galactose on the surface of cells of non-primate organs is the major xenoantigen responsible for hyperacute rejection in xenotransplantation. The antigen is synthesised by (alpha1, 3)galactosyl transferase. Humans lack this enzyme and their serum contains high levels of pre-existing natural antibody which recognises the structure and activates complement. We have evaluated in vitro the potential for delivery of this enzyme to sensitise human cells to complement attack as a gene therapy approach to cancer. Retrovirus-mediated delivery of (alpha1,3)galactosyl transferase resulted in high level expression which led to serum-mediated lysis of five human cell targets, including endothelial and primary melanoma cells. Lysis was specific for those cells expressing the antigen in a mixed cell population. The mechanism of cell lysis mimicked that involved in hyperacute rejection: activation of the classical complement pathway by natural antibody specific for galactose(alpha1,3)galactose. The degree of lysis was determined by both the level of specific antibody and the expression of glycophosphatidylinositol-linked complement regulatory proteins. We conclude that expression of (alpha1,3)galactosyl transferase is a promising new therapeutic approach for cancer gene therapy, avoiding toxicity problems associated with application of prodrugs and with the potential to elicit further immunological responses. (+info)The human antibody response to porcine xenoantigens is encoded by IGHV3-11 and IGHV3-74 IgVH germline progenitors. (2/163)
Preformed and induced Ab responses present a major immunological barrier to the use of pig organs for human xenotransplantation. We generated IgM and IgG gene libraries established from lymphocytes of patients treated with a bioartificial liver (BAL) containing pig hepatocytes and used these libraries to identify IgVH genes that encode human Ab responses to pig xenoantigens. Genes encoded by the VH3 family are increased in expression in patients following BAL treatment. cDNA libraries representing the VH3 gene family were generated, and the relative frequency of expression of genes used to encode the Ab response was determined at days 0, 10, and 21. Ig genes derived from the IGHV3-11 and IGHV3-74 germline progenitors increase in frequency post-BAL. The IGHV3-11 gene encodes 12% of VH3 cDNA clones expressed as IgM Abs at day 0 and 32.4-39.0% of cDNA clones encoding IgM Abs in two patients at day 10. IGHV3-11 and IGHV3-74 genes encoding IgM Abs in these patients are expressed without evidence of somatic mutation. By day 21, an isotype switch occurs and IGHV3-11 IgVH progenitors encode IgG Abs that demonstrate somatic mutation. We cloned these genes into a phagemid vector, expressed these clones as single-chain Abs, and demonstrated that the IGHV3-11 gene encodes Abs with the ability to bind to the gal alpha (1,3) gal epitope. Our results demonstrate that the xenoantibody response in humans is encoded by IgVH genes restricted to IGHV3-11 and IGHV3-74 germline progenitors. IgM Abs are expressed in germline configuration and IgG Abs demonstrate somatic mutations by day 21. (+info)The Th1/Th2 nature of concurrent immune responses to unrelated antigens can be independent. (3/163)
We tested the independence hypothesis, namely that the Th1/Th2 nature of concurrent immune responses, generated in the same secondary lymphoid organ to non-cross-reacting Ags, can be independently determined. Some infectious agents and some adjuvants contain modulatory molecules that affect the Th1/Th2 nature of immune responses in a non-Ag-specific manner. We therefore excluded infectious agents as Ags and the use of adjuvants to generate immune responses. We first show that the dose of xenogeneic RBC administered i.v. determines the Th1/Th2 nature of the splenic immune response. Low doses generate a virtually exclusive Th1 response, whereas a higher dose induces either a mixed Th1/Th2 or a predominantly Th2 response, and stimulates the production of specific Abs. We immunized individual mice simultaneously with a low dose of one kind of xenogeneic RBC and with a higher dose of another non-cross-reacting xenogeneic RBC and assessed the Th1/Th2 nature of the immune responses generated in the spleen to each kind of RBC. The Th1/Th2 nature of the response to each RBC in doubly immunized mice was indistinguishable from that of the corresponding immune response in singly immunized mice. We discuss the significance of our findings for understanding immune class regulation, and the possible reasons why such independence is not always seen. (+info)Class II MHC/peptide complexes are released from APC and are acquired by T cell responders during specific antigen recognition. (4/163)
T cell expression of class II MHC/peptide complexes may be important for maintenance of peripheral self-tolerance, but mechanisms underlying the genesis of class II MHC glycoproteins on T cells are not well resolved. T cell APC (T-APC) used herein were transformed IL-2-dependent clones that constitutively synthesized class II MHC glycoproteins. When pulsed with myelin basic protein (MBP) and injected into Lewis rats, these T-APC reduced the severity of experimental autoimmune encephalomyelitis, whereas unpulsed T-APC were without activity. Normal MBP-reactive clones cultured without APC did not express class II MHC even when activated with mitogens and exposed to IFN-gamma. However, during a 4-h culture with T-APC or macrophage APC, recognition of MBP or mitogenic activation of responder T cells elicited high levels of I-A and I-E expression on responders. Acquisition of class II MHC glycoproteins by responders was resistant to the protein synthesis inhibitor cycloheximide, coincided with transfer of a PKH26 lipophilic dye from APC to responders, and resulted in the expression of syngeneic and allogeneic MHC glycoproteins on responders. Unlike rested I-A- T cell clones, rat thymic and splenic T cells expressed readily detectable levels of class II MHC glycoproteins. When preactivated with mitogens, naive T cells acquired APC-derived MHC class II molecules and other membrane-associated proteins when cultured with xenogeneic APC in the absence of Ag. In conclusion, this study provides evidence that APC donate membrane-bound peptide/MHC complexes to Ag-specific T cell responders by a mechanism associated with the induction of tolerance. (+info)HLA-E and HLA-G expression on porcine endothelial cells inhibit xenoreactive human NK cells through CD94/NKG2-dependent and -independent pathways. (5/163)
Human NK cells contribute a significant role to host defense as well as xenogeneic cytotoxicity. Previous studies using human 721.221 cell line have shown that peptides derived from the leader sequence of the HLA-G binds and up-regulates the surface expression of HLA-E molecules, which was considered to consequently provide negative signals to human NK cells. However, the direct role of HLA-G in inhibiting human NK cells remains controversial. In this study, we showed that the expression of HLA-G or HLA-E in porcine endothelial cells directly protected sensitive porcine cells from human NK cell-mediated xenogeneic cytotoxicity. Ab blocking assays using F(ab')2 of the HLA class I-specific mAb PA2.6 indicated that the protection was directly mediated by the expression of HLA-G and HLA-E on the porcine cells. The HLA-E-mediated protection was blocked by anti-human CD94 Ab. In addition, the engagement of HLA-E lead to the phosphorylation of the CD94/NKG2 complex and the recruitment of SH2 domain-containing protein phosphatase 1 (SHP-1) to the complex. Therefore, HLA-E protected porcine cells from xenoreactive human NK cells through a CD94/NKG2-dependent pathway. In contrast, HLA-G inhibited human NK cells in the absence of CD94/NKG2 phosphorylation or SHP-1 recruitment, and the inhibition was not blocked by anti-CD94 Ab. Therefore, HLA-G protected porcine cells from human NK cells through a CD94/NKG2-independent pathway. These results demonstrated that both HLA-E and HLA-G could directly inhibit human NK cells in the absence of other endogenous HLA class I molecules. These results also have practical implications in preventing xenograft rejection mediated by human NK cells. (+info)Xenotransplantation: the importance of the Galalpha1,3Gal epitope in hyperacute vascular rejection. (6/163)
The transplantation of organs from other species into humans is considered to be a potential solution to the shortage of human donor organs. Organ transplantation from pig to human, however, results in hyperacute rejection, initiated by the binding of human natural antidonor antibody and complement. The major target antigen of this natural antibody is the terminal disaccharide Galalphal,3Gal, which is synthesized by Galbeta1,4GlcNAc alpha1,3-galactosyltransferase. Here we review our current knowledge of this key enzyme. A better understanding of structure, enzyme properties, and expression pattern of alpha1,3-galactosyltransferase has opened up several novel therapeutic approaches to prevent hyperacute vascular rejection. Cloning, and expression in vitro of the corresponding cDNA, has allowed to develop strategies to induce immune tolerance, and deplete or neutralize the natural xenoreactive antibody. Elucidation of the genomic structure has led to the production of transgenic animals that are lacking alpha1,3-galactosyltransferase activity. A detailed knowledge of the enzyme properties has formed the basis of approaches to modify donor organ glycosylation by intracellular competition. Study of the expression pattern of alpha1,3-galactosyltransferase has helped to understand the mechanism of hyperacute rejection in discordant xenotransplantation, and that of complement-mediated, natural immunity against interspecies transmission of retroviruses. (+info)Taking lessons from dendritic cells: multiple xenogeneic ligands for leukocyte integrins have the potential to stimulate anti-tumor immunity. (7/163)
Expression of large numbers of different costimulatory integrin ligands (CILs) attributes dendritic cells with an ability to induce primary anti-tumor immune responses. Here, we show that optimized gene transfer of the xenogeneic (human) CILs VCAM-1, MAdCAM-1 and ICAM-1 causes rapid and complete rejection of established mouse EL-4 tumors, and generates prolonged systemic anti-tumor immunity; whereas human E-cadherin weakly slows tumor growth. In each case the immune response was mediated by CD8+ T cells and NK cells, accompanied by augmented tumor-specific cytolytic T cell (CTL) activity involving both the perforin and Fas-ligand pathways. Adoptive transfer of splenocytes from cured mice rapidly cleared established tumors in recipients. The mechanism for CIL-mediated immunity is unknown, but may involve CTL-facilitated tumor lysis, since CTLs were generally twice as efficient at killing CIL-transfected tumor cells than parental tumor cells. Optimized CIL-based gene therapy may provide an approach to complement or replace conventional DC adoptive cell therapy for suppressing tumor growth. (+info)Naturally developing memory T cell xenoreactivity to swine antigens in human peripheral blood lymphocytes. (8/163)
Naturally developing xenospecific Abs are well-documented barriers to xenograft transplantation in humans, but whether analogous xenoreactive T cell immunity develops is not known. We used an enzyme-linked immunospot assay to determine the frequency and cytokine profiles of xenoreactive PBLs from a panel of human volunteers. Because naive T cells produce only IL-2 in short term culture, IFN-gamma production by this approach is a measure of a memory immune response. Stimulation of human PBLs or purified T lymphocytes with stimulator cells from inbred swine revealed a high frequency of IFN-gamma producers with 5-fold fewer IL-2 producers. In contrast, lymphocytes obtained from neonatal umbilical cord blood contained swine-specific IL-2 producers but few IFN-gamma producers, which is what one would expect to find with a naive phenotype. Moreover, PBLs from adults with a history of abstention from pork consumption responded to swine cells with a significantly lower frequency of IFN-gamma producers than PBLs from adults with unrestricted diets did, suggesting that pork consumption may result in priming of swine-specific T cell immunity. Our findings provide the first evidence for naturally occurring xenospecific T cell immunity in humans. The detected strength of this memory response suggests that it will present a formidable barrier to transplantation of swine organs. (+info)
Knock-Out Pigs Press Release
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Human thrombin and FXa mediate porcine endothelial cell activation; modulation by expression of TFPI-CD4 and hirudin-CD4 fusion...
Rolling adhesion of human NK cells to porcine endothelial cells mainly relies on CD49d-CD106 interactions1
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Interaction of antibody with Forssman antigen in guinea pigs: A mechanism of adaptation to antibody- and complement-mediated...
Heterophile | Definition of Heterophile by Merriam-Webster
TNF-α promotes human antibody-mediated complement-dependent cytotoxicity of porcine endothelial cells through downregulating...
An antigen-binding cell tumor. Heterophile cold hemagglutinin as a me by H Hiai, H Shisa et al.
Forssman antigen | definition of Forssman antigen by Medical dictionary
Xenoantibody | definition of xenoantibody by Medical dictionary
HETEROPHILE ANTIBODY REACTION IN INFECTIOUS MONONUCLEOSIS* | Annals of Internal Medicine | American College of Physicians
Use of molecular modeling and site-directed mutagenesis to define the structural basis for the immune response to carbohydrate...
Antibodies, heterophile synonyms, Antibodies, heterophile antonyms - FreeThesaurus.com
Most recent papers with the keyword Modifier gene vwf | Read by QxMD
New Century Labs | Heterophile Mono Screen
Efficiency of porcine endothelial cell infection with human cytomegalovirus depends on both virus tropism and endothelial cell...
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Engraftment and development of xenogeneic cells in normal mammals having reconstituted hematopoietic deficient immune...
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Mechanism of leflunomide-induced prevention of xenoantibody formation and xenograft rejection in the hamster to rat heart...
Sequence Detail
Primary Porcine Cells | Swine | Pig | Cell Applications
Xenotransplantation | 2018(07) articles
Goat Anti-Pig IgG Polyclonal Antibody (Unconjugated) | VWR
Erratum: Absence of Gal epitope prolongs survival of swine lungs in an ex vivo model of hyperacute rejection ...
Glycotope Receives Regulatory Approval For Glycoexpress(TM) Technology And Initiates First Clinical Trial With Lead Antibody GT...
Genetic modification of alpha-Gal expression in xenogeneic endothelial cells yields a complex immunological response - Leach Lab
What Is Xenotransplantation?
Xenotransplantation by Kaitlin Ball on Prezi
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anti-Porcine circovirus type 2 Capsid antibody | GeneTex
Complete chemoenzymatic synthesis of the Forssman antigen using novel glycosyltransferases identified in Campylobacter jejuni...
Immunological responses in mice to full-thickness corneal grafts engineered from porcine collagen
Elimination of N-Glycolylneuraminic Acid From Animal Products For Human Use - Patent application
Heterophile Antibody (Infectious Mononucleosis) by Latex Agglutination with Reflex to Titer | ARUP Lab Test Directory
Goat anti-Pig IgG-heavy and light chain cross adsorbed Antibody Affinity Purified - A100-205A - 0.5 mg - Bethyl Laboratories,...
SureStep™ Mono Test
QuickVue+ Mononucleosis Test | Quidel
Xenotransplantation - the transplant of organs or tissues between different species has become a major issue within the past
Jens Krause | IGB
Heterophile antigen
Heterophile antigens are antigens of similar nature, if not identical, that are present in different tissues in different ... Example: Forssman antigen, cross reacting microbial antigen so antibodies to these antigens produced by one species cross react ... but the hetereophile antigen is shared by different species. Other heterophile antigens are responsible for some diagnostic ... "Studies on heterophile antigen and antibody". Journal of Pathology. 24 (2): 217-240. doi:10.1002/path.1700240214. v t e v t e ( ...
History and naming of human leukocyte antigens
Rapaport FT, Kano K, Milgrom F (1968). "Heterophile antibodies in human transplantation". J. Clin. Invest. 47 (3): 633-42. doi: ... The Hu-1 antigens were renamed the Human-lymphoid (HL) allo-antigens (HL-As). Allo-antigen comes from the observation that a ... Human leukocyte antigens (HLA) began as a list of antigens identified as a result of transplant rejection. The antigens were ... As a result, scientists were able to identify a few antigens. At first the first antigens were called the Hu-1 antigens and ...
Heterophile antibody
Heterophile antibodies are antibodies induced by external antigens (heterophile antigens). Some cross-react with self-antigens ... Heterophile antigen An immunoassay is a biochemical test, frequently used in medical diagnostic testing, that measures the ... These are considered heterophile antibodies. In clinical diagnosis, the heterophile antibody test specifically refers to a ... Heterophile antibodies can cause significant interference in any immunoassay. The presence of a heterophile antibody is ...
Infectious mononucleosis
Elgh, F; Linderholm, M (1996). "Evaluation of six commercially available kits using purified heterophile antigen for the rapid ... Early antigen (EA) Anti-EA IgG appears in the acute phase of illness and disappears after 3 to 6 months. It is associated with ... EBV nuclear antigen (EBNA) Antibody to EBNA slowly appears 2 to 4 months after the onset of symptoms and persists for the rest ... The heterophile antibody test is a screening test that gives results within a day, but has significantly less than full ...
John Forssman
He is known for discovery of the "Forssman antigen", defined as a glycolipid heterophile antigen found on tissue cells of many ... His name is also associated with the following two terms: "Forssman antibody" (heterophile antibody) and "Forssman reaction", ... also referred to as a "Forssman antigen-antibody reaction". Über die Ursachen, welche die Wachsthumsrichtung der peripheren ...
Cross-reactivity
In immunology, cross-reactivity has a more narrow meaning of the reaction between an antibody and an antigen that differs from ... An example of helpful cross-reactivity is in heterophile antibody tests, which detect Epstein-Barr virus using antibodies with ... There can be cross-reactivity between the immune system and the antigens of two different pathogens, or between one pathogen ... For example, the tetanus toxin is a single protein macromolecular antigen but will stimulate many immune responses due to the ...
Heterophile antibody test
Heterophile also can mean that it is an antibody that reacts with antigens other than the antigen that stimulated it (an ... Elgh, F; Linderholm, M (1996). "Evaluation of six commercially available kits using purified heterophile antigen for the rapid ... It has the following characteristics: VCAs (Viral Capsid Antigen) of the IgM class, antibodies to EBV early antigen (anti-EA), ... An elevated heterophile antibody level may persist up to 1 year. The test is usually performed using commercially available ...
Index of immunology articles
Herd immunity Heterophile Heterophile antigen Heterosubtypic immunity Histamine Histocompatibility Human leukocyte antigen ... opsonization Antibody-dependent cell-mediated cytotoxicity Antibody-dependent enhancement Antigen Antigen presentation Antigen ... Fc receptor Fc receptor-like molecule FcεRI Fibroblast-like synoviocyte FITkit FluoroSpot Forssman antigen Fragment antigen- ... DAMPs Danger model Defensin Degranulation Dendritic cell Dextran 1 Dispanin Dog leukocyte antigen Drug resistance Duffy antigen ...
List of MeSH codes (D12.776)
... antigen, b-cell MeSH D12.776.377.715.548.950.500 - antigens, cd79 MeSH D12.776.377.715.647.100 - alpha-macroglobulins See List ... heterophile MeSH D12.776.377.715.548.114.224 - antibodies, monoclonal MeSH D12.776.377.715.548.114.224.570 - muromonab-cd3 MeSH ... antigen-antibody complex MeSH D12.776.377.715.548.114.301 - antitoxins MeSH D12.776.377.715.548.114.301.138 - antivenins MeSH ... antigens, polyomavirus transforming MeSH D12.776.624.664.520.420 - papillomavirus e7 proteins MeSH D12.776.624.664.520.750 - ...
List of MeSH codes (D12.776.124)
... antigen, b-cell MeSH D12.776.124.486.485.950.500 - antigens, cd79 MeSH D12.776.124.790.106.050 - alpha 1-antichymotrypsin MeSH ... heterophile MeSH D12.776.124.486.485.114.224 - antibodies, monoclonal MeSH D12.776.124.486.485.114.224.570 - muromonab-cd3 MeSH ... antigens, cd46 MeSH D12.776.124.486.274.920.250 - complement c1 inactivator proteins MeSH D12.776.124.486.274.920.250.500 - ... antigen-antibody complex MeSH D12.776.124.486.485.114.301 - antitoxins MeSH D12.776.124.486.485.114.301.138 - antivenins MeSH ...
Pharyngitis
The heterophile test can be used if this is suspected. Herpes simplex virus can cause multiple mouth ulcers. Measles Common ... Otherwise, a rapid antigen detection test (RADT) or throat swab is recommended. Other conditions that can produce similar ...
Pregnancy test
Direct measurement of antigens, such as hCG, was made possible after the invention of the radioimmunoassay in 1959. ... heterophile antibodies, enterocystoplasties, gestational trophoblastic diseases (GTD), and gestational trophoblastic neoplasms ...
Fatal Respiratory Diphtheria in a U.S. Traveler to Haiti ---
Pennsylvania, 2003
Epstein-Barr Virus (EBV) Infectious Mononucleosis (Mono) Workup: Approach Considerations, Laboratory Studies, Imaging Studies
These heterophile antibodies react to antigens from animal RBCs.. * Sheep RBCs agglutinate in the presence of heterophile ... Viral capsid antigen (VCA) - Anti-VCA IgM and anti-VCA IgG antibodies directed against EBV viral capsid antigen are present at ... Heterophile antibodies. Since heterophile antibodies typically return quicker than EBV specific antibodies, it is the test of ... Patients who remain heterophile negative after 6 weeks with a mononucleosis illness should be considered as having heterophile- ...
NHANES 2011-2012: Tuberculosis - Quantiferon In Gold Data Documentation, Codebook, and Frequencies
LBXTBA - TB Antigen result. Variable Name: LBXTBA. SAS Label: TB Antigen result. English Text: TB Antigen result. Target: Both ... The effect of heterophile antibodies in the QuantiFERON®-TB Gold IT ELISA is minimized by the addition of normal mouse serum to ... LBXTBA (TB antigen value) minus LBXTBN (Nil) value must be =, 0.35 IU gamma interferon (IF)/ml AND LBXTBA (TB antigen value) ... TB Antigen tube and a Mitogen tube (positive control). The tubes are shaken to mix antigen with the whole blood and incubated ...
Law Document English View | Ontario.ca
Hepatitis Associated Antigen or Antibody Immunoassay (e.g. hepatitis B surface antigen or antibody, hepatitis B anticore ... Heterophile Antibodies - screen (slide or single tube) - with or without absorption.. 70. Virus antibodies - hemagglutination ... Prostate Specific Antigen (PSA), Total.. O. Reg. 46/98, s. 2; O. Reg. 62/02, s. 1; O. Reg. 239/04, s. 6; O. Reg. 71/07, s. 2; O ... 5. That a Hepatitis B surface antigen test not be performed if the person requesting the test indicates that the test is for a ...
Women in Poultry Science
Infectious Mononucleosis (IM) in Emergency Medicine: Background, Pathophysiology, Epidemiology
Pathogen Safety Data Sheets: Infectious Substances - Epstein-Barr virus - Canada.ca
Tests for heterophile antibodies in mononucleosis, antibodies against viral capsid antigen (VCA), antibodies to EBV nuclear ... Direct detection of the viral antigen can be done by staining for EBNA 1 using anti complement immunofluorescence; viral RNA or ... antigen (EBNA) can also be usedFootnote 1Footnote 2Footnote 4. ...
Hypertriglyceridemia during infectious mononucleosis | Research Square
The serum heterophile antibody test was negative; however, the IgM against the viral capsid antigen (VCA) of EBV was positive. ... followed by antibodies against EBV nuclear antigen (EBNA), with a progressive disappearance of IgM against VCA, thereby ... was considered when the usual clinical syndrome was accompanied by positive IgM antibodies against the viral capsid antigen of ...
TC83 replicon vectored vaccine provides protection against Junin virus in guinea pigs - Fingerprint
- UTMB Health Research...
WHO EMRO | Trend and seroprevalence of Epstein-Barr virus in Bahrain: 2001-2015 | Volume 23, issue 12 | EMHJ volume 23, 2017
... for heterophile antibodies), and enzyme immune assay for detection of early antigens, the viral capsid antigens (VCA) and the ... The presence or absence of EBV viral capsid antigen (VCA) IgG, VCA IgM and EBV nuclear antigen (EBNA) IgG antibodies was ... EBV nuclear antigen (EBNA). Full automation of EBV serological diagnosis is important for routine diagnostic laboratories (6). ...
Epstein-Barr Virus - EBV | Choose the Right Test
... and Epstein-Barr nuclear antigen (EBNA) can be used to follow up on negative or positive heterophile antibody test results. ... Heterophile Antibody Test. The heterophile antibody test (Monospot) is a standard diagnostic test for EBV IM in patients with ... Components: EBV antibody to VCA, IgG and IgM; EBV antibody to nuclear antigen, IgG; and EBV antibody to early D antigen (EA-D ... the heterophile antibody test should not be used in children younger than 5 years, most of whom do not produce the heterophile ...
Infectious Mononucleosis/Reactive Lymphocytes - A Laboratory Guide to Clinical Hematology
DeCS
Heterologous Antigens Heterophil Antigens Heterophile Antigen Heterophile Antigens Paul Bunnell Antigens Paul-Bunnell Antigens ... Heterologous Antigens. Heterophil Antigens. Heterophile Antigen. Heterophile Antigens. Paul Bunnell Antigens. Paul-Bunnell ... Antigens, Heterophile Entry term(s). Antigens, H-D Antigens, Hanganutziu-Deicher Antigens, Heterogenetic Antigens, Heterologous ... Antigens, Heterophil Antigens, Paul-Bunnell Antigens, Xenogeneic Antigens, Xenogenic H D Antigens H-D Antigens Hanganutziu ...
IMSEAR at SEARO: Search
Pediatric Puzzler: Unremitting fever
You opt to wait for results of viral culture and rapid viral antigen testing from a nasopharyngeal swab and to reexamine the ... Could this be Epstein-Barr virus (EBV) infection? A Monospot (rapid heterophile antibody) test is negative, but the test can be ... Rapid viral antigen testing is negative for respiratory syncytial virus, adenovirus, parainfluenza, and influenza. The rectal ...
Activation of natural killer cells and macrophages by porcine endothelial cells augments specific T-cell xenoresponse -...
DeCS 2011 - December 22, 2011 version
Upper Respiratory Tract Infection: Practice Essentials, Background, Pathophysiology
Services - McCone County Health CenterMcCone County Health Center
35.00 H PYLORI ANTIGEN FECES $60.00 HEPATITIS B SURFACE AG EIA $69.00 HBSAG NEUTRALIZATION CONFIRM $144.00 INFLUENZA A/B AG EIA ... 125.25 HETEROPHILE ANTIBODY SCREEN $24.00 IMMUNOASSAY INFECTIOUS AGENT $158.50 IMMUNOFIX E-PHORESIS SERUM $124.00 INHIBIN A $ ... 148.00 PROTEIN C ANTIGEN PLASMA $275.00 PROTIEN S ACTIVITY $182.75 D-DIMER $150.00 LAP SCORE $63.00 PROTHROMBIN TIME $50.50 ... 385.00 COMPLIMENT ANTIGEN C4 $48.00 ESTRASE INHIBITOR FUNCTION C1 $62.00 CCP ANTIBODY $66.50 DNA ANTIBODY NATIVE $77.00 NUCEAR ...
Interference Evaluation Heterophile, Beta-Human Chorionic Gonadotropin, Serum - Mayo Clinic Laboratories | Pediatric Catalog
Some patients, due to exposure to animal antigens, have developed antibodies that interfere with immunoassay testing. These ... Heterophile antibody evaluation consists of pretreatment with commercial heterophile antibody blocking reagents, testing on an ... Test ID IEHCG Interference Evaluation Heterophile, Beta-Human Chorionic Gonadotropin, Serum Ordering Guidance. If "HCG Total OB ... The presence of heterophile antibody interference in the Roche Elecsys assay is suspected when 1 or more of the following are ...
Pesquisa | Prevenção e Controle de Câncer
... which include testing for specific IgM and IgG antibodies against viral capsid antigens, early antigens and EBV nuclear antigen ... However, up to 10% of patients with IM are heterophile negative. Heterophile-negative patients who have lymphocytosis or ... and a rapid heterophile antibody test. The heterophile antibody test has a sensitivity of 87% and specificity of 91% but can ... The heterophile antibody (also known as the Monospot) test is a useful screening tool for infectious mononucleosis (IM) ...
Monospot
Heterophile Antibody Test, Heterophil Agglutination Tube Test, Paul-Bunnell Test, Mononucleosis Heterophile Test, Heterophile ... Heterophile Antibodies (C0003248) Definition (CSP). antibody produced by the introduction of heterophile antigen into a species ... heterophile antibody, heterophile ab, heterophil antibody, heterophile antibodies, Heterophile Ab, Heterophile Antibody, ... HETEROPHILE ANTIBODIES SCREEN, Screening for heterophile antibodies, HETEROPHILE ANTIBODY SCREEN, Heterophile antibody screen. ...
The comparative analysis of limfogramy's indicators in adults with verified allegropathology on the background of replicative...
All patients were screened for the presence of worms (IEA for the presence of immunoglobulins A, M, G to antigens for Giardia, ... Votava, D. Bartosova, A. Krchnakova [et al.] «Diagnostic importance of heterophile antibodies and immunoglobulins IgA, IgE, IgM ... and low-avidity IgG against Epstein-Barr virus capsid antigen in children», Acta Virol. -1996.- V.40.- Р. 99-101. ...
Browse by Publication Year : 2020-2029 : HistoryofMedicine.com
Epstein-Barr virus (EBV) antibodies-viral capsid antigen IgM and IgG, and EBV-associated nuclear antigen antibodies-were also ... A rapid point-of-care test for heterophile antibodies-the Monospot test-was negative, a full blood count with autodifferential ... A rapid antigen detection test for streptococcus was negative, and because of exudates on the patients tonsils and very ...
Epstein-Barr Virus (EBV) Infectious Mononucleosis (Mono): Background, Pathophysiology, Epidemiology
Laboratory diagnosis of epstein barr virus ebv )? how do you interpret epstein barr results, epstein-barr virus (ebv), also...
... heterophile antibodies, and IgG antibodies to the EBV early antigen-diffuse (EA-D) and nuclear antigen (EBNA-1). The use of all ... viral capsid antigen (VCA) IgG, VCA IgM, and EBV nuclear antigen 1 IgG chemiluminescent immunoassays for detection of EBV ... EBV nuclear antigen (EBNA) IgG: Just like the VCA IgG these increase after the initial infection and then stay elevated for ... Here are the results of my EBV test: EBV Ab VCA, IgG, 260 EBV Ab VCA, IgM, less than 36 EBV Early Antigen Ab, IgG, 93.2 EBV ...
DeCS 2014 - December 16, 2014 version
DeCS 2008 - versión 17 de Marzo de 2008
DeCS 2010 - February 12, 2010 version
MonospotContaining heterophile antibodiesViralEBNAMononucleosisAssaysDetectionImmunoassaysProteinsNuclearPaul-BunnellSerumTestInfectionRecombinantReactSerologyInduceInfluenzaSpeciesProteinDiagnosticDescriptorNegativeAcuteTestsExposureLymphocytesOccurSpecificAssessmentPresencePositiveBloodTypePatient
Monospot7
- Agglutination of horse RBCs on exposure to heterophile antibodies is the basis of the Monospot test. (medscape.com)
- Rapid heterophile antibody testing (also referred to as Monospot) is often used as a first-line test in the diagnosis of EBV IM because it is inexpensive and has a fast turnaround. (arupconsult.com)
- Heterophile antibody testing (also known as Monospot) is often used as a first-line test in the diagnosis of Epstein-Barr virus (EBV) infectious mononucleosis (IM). (arupconsult.com)
- The heterophile antibody test (Monospot) is a standard diagnostic test for EBV IM in patients with symptoms of IM. (arupconsult.com)
- A Monospot (rapid heterophile antibody) test is negative, but the test can be unreliable in young children. (contemporarypediatrics.com)
- The heterophile antibody (also known as the Monospot) test is a useful screening tool for infectious mononucleosis (IM) resulting from primary Epstein-Barr virus (EBV) infection. (bvsalud.org)
- Don't perform heterophile antibody (monospot) testing to diagnose acute Epstein-Barr virus (EBV) infection in children under 5 years of age. (labpulse.com)
Containing heterophile antibodies2
- Manufacturers add blocking agents to their reagents, but occasional patient samples containing heterophile antibodies are incompletely blocked. (testcatalog.org)
- Heterophile blocking tube (HBT) treatment is used for troubleshooting samples that yield results that are either non-linear or do not match the clinical picture of the patient and are suspected of containing heterophile antibodies. (testcatalog.org)
Viral5
- IgM and IgG antibodies directed against viral capsid antigen has high sensitivity and specificity. (medscape.com)
- Viral capsid antigen (VCA) - Anti-VCA IgM and anti-VCA IgG antibodies directed against EBV viral capsid antigen are present at the onset of infection. (medscape.com)
- The presence or absence of EBV viral capsid antigen (VCA) IgG, VCA IgM and EBV nuclear antigen (EBNA) IgG antibodies was recorded. (who.int)
- Heterophile-negative patients who have lymphocytosis or atypical lymphocytes on peripheral blood smear should be further tested for EBV serologies, which include testing for specific IgM and IgG antibodies against viral capsid antigens, early antigens and EBV nuclear antigen proteins. (bvsalud.org)
- The Epstein-Barr virus is a very common infection The diagnosis of infection due to herpesviruses can be performed by several laboratory techniques, including virus culture, demonstration of virus-specific cellular antigens, detection of virus-induced serum antibodies, detection of viral nucleic acids (using radiolabeled probes), or visualization of histologic changes in infected tissue How Do You Get Epstein-Barr Virus in the First Place? (heldeccola.com)
EBNA3
- EBV Nuclear antigen (EBNA) - This is a protein expressed only when the virus begins to establish latency. (medscape.com)
- EBV nuclear antigen (EBNA) IgG: Just like the VCA IgG these increase after the initial infection and then stay elevated for life. (heldeccola.com)
- EBNA IgG (Epstein Barr Nuclear Antigen) - Indicates a history of infection in non-acute cases. (heldeccola.com)
Mononucleosis9
- Since heterophile antibodies typically return quicker than EBV specific antibodies, it is the test of choice in most clinical settings to determine if an individual has infectious mononucleosis. (medscape.com)
- One must consider that the heterophile antibody test results may be negative early in the course of EBV infectious mononucleosis. (medscape.com)
- Patients who remain heterophile negative after 6 weeks with a mononucleosis illness should be considered as having heterophile-negative infectious mononucleosis. (medscape.com)
- These may be considered in patients with suspected infectious mononucleosis who have a negative heterophile test. (medscape.com)
- This test is often prescribed for adolescents exhibiting symptoms typical of mononucleosis, such as fever, fatigue, sore throat, swollen glands, etc Mono Test is intended for the qualitative detection of infectious mononucleosis heterophile antibodies in serum, plasma or whole blood as an aid in the diagnosis of infectious mononucleosis. (keresekvapnetbacio.com)
- SUMMARY AND EXPLANATION OF TEST Our Mono test detects heterophile antibodies in the blood that are produced by the immune system as a response to fighting the Epstein-Barr Virus (EBV), the most common cause of mononucleosis. (keresekvapnetbacio.com)
- For the qualitative detection of infectious mononucleosis heterophile antibodies in whole blood, serum or plasma. (carolinachemistries.com)
- Herpesviruses: Human herpesvirus 6 - HHV-6: ELISA-VIDITEST and IF-VIDITEST anti-HHV-6 kits are intended for serological diagnosis of diseases associated with HHV-6 infection, such as exanthema subitum, acute respiratory illnesses, diarrhoea with fever and febrile seizures in infants, heterophile antibody-negative infectious mononucleosis in children, also interstitial pneumonia, encephalitis, meningitis, hepatitis and aplastic anemia in immunodeficient patients. (mobitec.de)
- A small percentage of individuals will develop a negative heterophile-antibody infectious mononucleosis syndrome. (frenovobio.com)
Assays1
- Among immunometric assays, human chorionic gonadotropin (hCG) assays have been found uniquely susceptible to heterophile antibody interference, resulting in occasional false-positive results. (testcatalog.org)
Detection3
- However, previous studies indicate that sensitivity of p24 antigen detection for AHI might not exceed 80% ( 7 ). (cdc.gov)
- [2] Otherwise, a rapid antigen detection test (RADT) or throat swab is recommended. (mdwiki.org)
- Rapid detection of group A streptococcal antigen directly from throat swab specimens. (carolinachemistries.com)
Immunoassays2
- Heterophile antibodies in plasma of certain individuals are known to cause interference with immunoassays. (cdc.gov)
- The Centers for Disease Control and Prevention began addressing this problem by updating recommendations for the laboratory diagnosis of HIV in healthcare settings to include initial fourth generation HIV-1 p24 antigen-based immunoassays ( 6 ). (cdc.gov)
Proteins3
- The QuantiFERON ® -TB Gold IT test is a test for Cell Mediated Immune (CMI) responses to petide antigens that simulate mycobacterial proteins. (cdc.gov)
- The test is used to detect proteins in the blood called heterophile antibodies that are produced by the immune system in response to an Epstein-Barr virus (EBV) infection, the most common cause of mono Mono Test. (keresekvapnetbacio.com)
- Study of the immune system analyses of blood that employ antigens (foreign proteins) and antibodie. (healthsfirst.in)
Nuclear1
- Don't order antinuclear antibody (ANA) and extractable nuclear antigen (ENA) tests unless the patient is suspected to have a connective tissue disease. (labpulse.com)
Paul-Bunnell2
- Sheep RBCs agglutinate in the presence of heterophile antibodies and are the basis for the Paul-Bunnell test. (medscape.com)
- These antibodies are directed against a wide variety of interspecies-specific antigens, the best known of which are Forssman, Hanganutziu-Deicher (H-D), and Paul-Bunnell (P-B). Incidence of antibodies to these antigens--i.e., the phenomenon of heterophile antibody response--is useful in the serodiagnosis, pathogenesis, and prognosis of infection and latent infectious states as well as in cancer classification. (fpnotebook.com)
Serum1
- The effect of heterophile antibodies in the QuantiFERON ® -TB Gold IT ELISA is minimized by the addition of normal mouse serum to the green diluent and the use of F(ab')2 monoclonal antibody fragments as the IFN-γ capture antibody coated the microplate wells. (cdc.gov)
Test16
- 5. That a Hepatitis B surface antigen test not be performed if the person requesting the test indicates that the test is for a prenatal assessment. (ontario.ca)
- however, the diagnosis should be confirmed with a heterophile antibody test or through EBV-specific antibodies. (medscape.com)
- Once a reactive heterophile antibody is present, it is not necessary to test for specific antibodies to EBV. (medscape.com)
- In patients with a compatible syndrome and negative heterophile antibodies, the test can be repeated if the patient is early in their clinical course. (medscape.com)
- A test is considered positive for an IFN-γ response to the TB Antigen tube that is significantly above the Nil IFN-γ IU/mL value. (cdc.gov)
- After incubation of the blood with antigens for 16 to 24 hours and in conjunction with a regularly scheduled shipment, specimens were sent to a contracted laboratory on frozen refrigerant packs or dry ice where the QFT-GIT test for tuberculosis infection was conducted. (cdc.gov)
- In 1932, the association between the disease syndrome and a positive heterophile antibody test was recognized. (medscape.com)
- EBV-specific serology can also be used in the place of heterophile antibody testing as an initial test. (arupconsult.com)
- Although the CDC does not recommend the heterophile antibody test for general use, this test is often used as a first-line test because it is fast and inexpensive. (arupconsult.com)
- In general, the heterophile antibody test should not be used in children younger than 5 years, most of whom do not produce the heterophile antibody. (arupconsult.com)
- EBV-specific serology testing may also be used to diagnose EBV IM in place of a heterophile antibody test. (arupconsult.com)
- The 4 tests are: EA IgG (Early Antigen) - this is the most important test and it's often missed. (heldeccola.com)
- The main technical methods for COVID-19 testing include reverse transcription loop-mediated isothermal amplification (RT-LAMP), RT-PCR, serology test and antigen test. (ipscio.com)
- The heterophile test can be used if this is suspected. (mdwiki.org)
- An in vitro rapid qualitative test that detects influenza type A and type B antigens directly from nasal swab, nasopharyngeal swab and nasal aspirate/wash specimens. (carolinachemistries.com)
- NOTE: The Coronavirus Aid, Relief, and Economic Security (CARES) Act require every CLIA certified COVID-19 testing site to report every diagnostic and screening test result (both positive and negative results) performed to detect SARS-CoV-2 or to diagnose a possible case of COVID-19 (e.g., molecular, antigen, antibody) to the appropriate state or local public health department, based on the individual's residence. (symbioticainc.com)
Infection4
- Epstein-Barr virus (EBV) infection induces specific antibodies to EBV and various unrelated non-EBV heterophile antibodies. (medscape.com)
- [ 32 ] Although anti-VCA IgM is highly suggestive of acute EBV infection, other herpes viruses (eg, cytomegalovirus) can induce IgM antibodies to cell lines that express EBV antigens. (medscape.com)
- To determine the prevalence of TB infection, NHANES participants 6 years of age and older who consented to this component were skin tested with a tuberculin-purified protein derivative (PPD) product, tubersol, a commercially available antigen. (cdc.gov)
- B-cell infection caused by EBV leads to the transformation of the B cells to immortal plasmacytoid cells, which secrete a wide variety of immunoglobulins (eg, heterophile antibodies). (medscape.com)
Recombinant1
- Small synthetic peptides that mimic surface antigens of pathogens and are immunogenic, or vaccines manufactured with the aid of recombinant DNA techniques. (nih.gov)
React2
- These heterophile antibodies react to antigens from animal RBCs. (medscape.com)
- Antibodies that react with self-antigens ( AUTOANTIGENS ) of the organism that produced them. (nih.gov)
Serology1
- If results are negative for EBV, serology tests for other virus-specific antigens (eg, cytomegalovirus , Toxoplasma gondii , human herpesvirus 6 , and HIV ) may be considered. (arupconsult.com)
Induce1
- Antibodies, especially IGE, that bind to tissue of the same species so that ANTIGENS induce release of HISTAMINE and other vasoactive agents. (ouhsc.edu)
Influenza1
- This study demonstrated that pregnant women were able to respond to an original myxovirus antigen, influenza A/New Jersey/8/76, in a manner equivalent to nonpregnant, age-matched controls. (elsevier.com)
Species3
- They are cross-reacting antigens found in phylogenetically unrelated species. (bvsalud.org)
- antibody produced by the introduction of heterophile antigen into a species which does not carry it. (fpnotebook.com)
- Antibodies elicited in a different species from which the antigen originated. (fpnotebook.com)
Protein1
- Prostate specific antigen (PSA) is a protein made by the prostate. (mjdrdypu.org)
Diagnostic2
- Reactive heterophile antibodies in a patient with the typical symptoms are diagnostic of EBV and further testing for specific antibodies to EBV is not necessary. (medscape.com)
- A diagnostic dilemma arises when the patient has clinical and laboratory features of IM, but is both heterophile negative and seronegative for IM, as illustrated in this case presentation. (bvsalud.org)
Descriptor1
- Antigens, Bacterial" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (wakehealth.edu)
Negative2
- 0.5 IU/mL) indicates an Indeterminate result when a blood sample also has a negative response to the TB antigens. (cdc.gov)
- However, up to 10% of patients with IM are heterophile negative. (bvsalud.org)
Acute1
- Early antigen (EA) - Anti-EA IgG appears during the acute phase and falls to undetectable levels after 3 to 6 months. (medscape.com)
Tests1
- Heterophile antibodies against these tests so important role of dtc, some different antigenic site with suppressive functionality on? (redcedarassociates.com)
Exposure1
- Some patients, due to exposure to animal antigens, have developed antibodies that interfere with immunoassay testing. (testcatalog.org)
Lymphocytes1
- Individuals infected with M. tuberculosis complex organisms ( M. tuberculosis, M. bovis, M. africanum, M. microti, M. canetti ) usually have lymphocytes in their blood that recognize these and other mycobacterial antigens. (cdc.gov)
Occur2
- Elevated levels of IFN-γ in the Nil sample may occur with the presence of heterophile antibodies, or to intrinsic IFN-γ secretion. (cdc.gov)
- Analytical errors occur due to heterophile antibodies and macro-TSH. (mjdrdypu.org)
Specific2
- The Nil samples adjust for background, heterophile antibody effects, or non-specific IFN-γ in blood samples. (cdc.gov)
- Antibodies against specific EBV antigens and a number of autoantibodies also are produced. (medscape.com)
Assessment1
- Finally, assessment of an analyte such as hCG with an alternative assay will often lead to apparent discrepant results in the presence of a heterophile antibody, as heterophile antibodies often interact differently with alternative assay antibodies. (testcatalog.org)
Presence1
- Dilution of the specimen prior to assay performance often yields unexpected non-linear results in the presence of interfering substances such as heterophile antibodies. (testcatalog.org)
Positive1
- The QuantiFERON ® -TB Gold IT system uses specialized blood collection tubes, which are used to collect whole blood via venipuncture, which include a Nil control tube, TB Antigen tube and a Mitogen tube (positive control). (cdc.gov)
Blood2
- The tubes are shaken to mix antigen with the whole blood and incubated at 37° C + 1° C for 16 to 24 hours. (cdc.gov)
- For each subject, 1 mL of blood was collected by venipuncture directly into each of three QFT GIT blood collection tubes in the order Nil (grey cap), TB Antigen (red cap), Mitogen (purple cap). (cdc.gov)
Type1
- Urticarial vasculitis is a type III hypersensitivity reaction in which antigen-antibody complexes are deposited in the vascular lumina. (medscape.com)
Patient1
- Un patient de 28 ans se présente au service des urgences pour prise en charge d'une douleur localisée au niveau de l'hypochondre gauche et du flanc gauche, apparue 48h avant son passage aux urgences. (bvsalud.org)