OrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentGeographicals
Antibodies, HeterophileAntigens, HeterophileInfectious MononucleosisForssman AntigenImmunoconglutininsAntigensHemagglutination TestsLatex Fixation TestsFalse Positive ReactionsImmunoassayAntigens, BacterialAntigens, NeoplasmAntigens, SurfaceHerpesvirus 4, HumanImmunoglobulin MReagent Kits, DiagnosticImmunoglobulin GAntigens, ViralAntibodies, ViralAntigens, ProtozoanAntigens, Polyomavirus TransformingHLA AntigensEpstein-Barr Virus InfectionsEpstein-Barr Virus Nuclear AntigensBaltimorePancreaticoduodenectomy
Antibodies, Heterophile
Antibodies elicited in a different species from which the antigen originated. These antibodies are directed against a wide variety of interspecies-specific antigens, the best known of which are Forssman, Hanganutziu-Deicher (H-D), and Paul-Bunnell (P-B). Incidence of antibodies to these antigens--i.e., the phenomenon of heterophile antibody response--is useful in the serodiagnosis, pathogenesis, and prognosis of infection and latent infectious states as well as in cancer classification.
Antigens, Heterophile
Antigens stimulating the formation of, or combining with heterophile antibodies. They are cross-reacting antigens found in phylogenetically unrelated species.
Infectious Mononucleosis
A common, acute infection usually caused by the Epstein-Barr virus (HERPESVIRUS 4, HUMAN). There is an increase in mononuclear white blood cells and other atypical lymphocytes, generalized lymphadenopathy, splenomegaly, and occasionally hepatomegaly with hepatitis.
Forssman Antigen
A glycolipid, cross-species antigen that induces production of antisheep hemolysin. It is present on the tissue cells of many species but absent in humans. It is found in many infectious agents.
Immunoconglutinins
Autoantibodies directed against newly-formed EPITOPES created as the COMPLEMENT cascade is activated and the proteins involved change their conformations.
Antigens
Substances that are recognized by the immune system and induce an immune reaction.
Hemagglutination Tests
Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed)
Latex Fixation Tests
Passive agglutination tests in which antigen is adsorbed onto latex particles which then clump in the presence of antibody specific for the adsorbed antigen. (From Stedman, 26th ed)
False Positive Reactions
Positive test results in subjects who do not possess the attribute for which the test is conducted. The labeling of healthy persons as diseased when screening in the detection of disease. (Last, A Dictionary of Epidemiology, 2d ed)
Immunoassay
A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.
Antigens, Bacterial
Substances elaborated by bacteria that have antigenic activity.
Antigens, Neoplasm
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
Antigens, Surface
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
Herpesvirus 4, Human
The type species of LYMPHOCRYPTOVIRUS, subfamily GAMMAHERPESVIRINAE, infecting B-cells in humans. It is thought to be the causative agent of INFECTIOUS MONONUCLEOSIS and is strongly associated with oral hairy leukoplakia (LEUKOPLAKIA, HAIRY;), BURKITT LYMPHOMA; and other malignancies.
Immunoglobulin M
A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.
Reagent Kits, Diagnostic
Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.
Immunoglobulin G
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
Antigens, Viral
Substances elaborated by viruses that have antigenic activity.
Antibodies, Viral
Immunoglobulins produced in response to VIRAL ANTIGENS.
Antigens, Protozoan
Any part or derivative of any protozoan that elicits immunity; malaria (Plasmodium) and trypanosome antigens are presently the most frequently encountered.
Antigens, Polyomavirus Transforming
Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.
HLA Antigens
Antigens determined by leukocyte loci found on chromosome 6, the major histocompatibility loci in humans. They are polypeptides or glycoproteins found on most nucleated cells and platelets, determine tissue types for transplantation, and are associated with certain diseases.
Epstein-Barr Virus Infections
Infection with human herpesvirus 4 (HERPESVIRUS 4, HUMAN); which may facilitate the development of various lymphoproliferative disorders. These include BURKITT LYMPHOMA (African type), INFECTIOUS MONONUCLEOSIS, and oral hairy leukoplakia (LEUKOPLAKIA, HAIRY).
Epstein-Barr Virus Nuclear Antigens
Nuclear antigens encoded by VIRAL GENES found in HUMAN HERPESVIRUS 4. At least six nuclear antigens have been identified.
Baltimore
Pancreaticoduodenectomy
The excision of the head of the pancreas and the encircling loop of the duodenum to which it is connected.

Induction of complement attack on human cells by Gal(alpha1,3)Gal xenoantigen expression as a gene therapy approach to cancer. (1/163)

Galactose(alpha1,3)galactose on the surface of cells of non-primate organs is the major xenoantigen responsible for hyperacute rejection in xenotransplantation. The antigen is synthesised by (alpha1, 3)galactosyl transferase. Humans lack this enzyme and their serum contains high levels of pre-existing natural antibody which recognises the structure and activates complement. We have evaluated in vitro the potential for delivery of this enzyme to sensitise human cells to complement attack as a gene therapy approach to cancer. Retrovirus-mediated delivery of (alpha1,3)galactosyl transferase resulted in high level expression which led to serum-mediated lysis of five human cell targets, including endothelial and primary melanoma cells. Lysis was specific for those cells expressing the antigen in a mixed cell population. The mechanism of cell lysis mimicked that involved in hyperacute rejection: activation of the classical complement pathway by natural antibody specific for galactose(alpha1,3)galactose. The degree of lysis was determined by both the level of specific antibody and the expression of glycophosphatidylinositol-linked complement regulatory proteins. We conclude that expression of (alpha1,3)galactosyl transferase is a promising new therapeutic approach for cancer gene therapy, avoiding toxicity problems associated with application of prodrugs and with the potential to elicit further immunological responses.  (+info)

The human antibody response to porcine xenoantigens is encoded by IGHV3-11 and IGHV3-74 IgVH germline progenitors. (2/163)

Preformed and induced Ab responses present a major immunological barrier to the use of pig organs for human xenotransplantation. We generated IgM and IgG gene libraries established from lymphocytes of patients treated with a bioartificial liver (BAL) containing pig hepatocytes and used these libraries to identify IgVH genes that encode human Ab responses to pig xenoantigens. Genes encoded by the VH3 family are increased in expression in patients following BAL treatment. cDNA libraries representing the VH3 gene family were generated, and the relative frequency of expression of genes used to encode the Ab response was determined at days 0, 10, and 21. Ig genes derived from the IGHV3-11 and IGHV3-74 germline progenitors increase in frequency post-BAL. The IGHV3-11 gene encodes 12% of VH3 cDNA clones expressed as IgM Abs at day 0 and 32.4-39.0% of cDNA clones encoding IgM Abs in two patients at day 10. IGHV3-11 and IGHV3-74 genes encoding IgM Abs in these patients are expressed without evidence of somatic mutation. By day 21, an isotype switch occurs and IGHV3-11 IgVH progenitors encode IgG Abs that demonstrate somatic mutation. We cloned these genes into a phagemid vector, expressed these clones as single-chain Abs, and demonstrated that the IGHV3-11 gene encodes Abs with the ability to bind to the gal alpha (1,3) gal epitope. Our results demonstrate that the xenoantibody response in humans is encoded by IgVH genes restricted to IGHV3-11 and IGHV3-74 germline progenitors. IgM Abs are expressed in germline configuration and IgG Abs demonstrate somatic mutations by day 21.  (+info)

The Th1/Th2 nature of concurrent immune responses to unrelated antigens can be independent. (3/163)

We tested the independence hypothesis, namely that the Th1/Th2 nature of concurrent immune responses, generated in the same secondary lymphoid organ to non-cross-reacting Ags, can be independently determined. Some infectious agents and some adjuvants contain modulatory molecules that affect the Th1/Th2 nature of immune responses in a non-Ag-specific manner. We therefore excluded infectious agents as Ags and the use of adjuvants to generate immune responses. We first show that the dose of xenogeneic RBC administered i.v. determines the Th1/Th2 nature of the splenic immune response. Low doses generate a virtually exclusive Th1 response, whereas a higher dose induces either a mixed Th1/Th2 or a predominantly Th2 response, and stimulates the production of specific Abs. We immunized individual mice simultaneously with a low dose of one kind of xenogeneic RBC and with a higher dose of another non-cross-reacting xenogeneic RBC and assessed the Th1/Th2 nature of the immune responses generated in the spleen to each kind of RBC. The Th1/Th2 nature of the response to each RBC in doubly immunized mice was indistinguishable from that of the corresponding immune response in singly immunized mice. We discuss the significance of our findings for understanding immune class regulation, and the possible reasons why such independence is not always seen.  (+info)

Class II MHC/peptide complexes are released from APC and are acquired by T cell responders during specific antigen recognition. (4/163)

T cell expression of class II MHC/peptide complexes may be important for maintenance of peripheral self-tolerance, but mechanisms underlying the genesis of class II MHC glycoproteins on T cells are not well resolved. T cell APC (T-APC) used herein were transformed IL-2-dependent clones that constitutively synthesized class II MHC glycoproteins. When pulsed with myelin basic protein (MBP) and injected into Lewis rats, these T-APC reduced the severity of experimental autoimmune encephalomyelitis, whereas unpulsed T-APC were without activity. Normal MBP-reactive clones cultured without APC did not express class II MHC even when activated with mitogens and exposed to IFN-gamma. However, during a 4-h culture with T-APC or macrophage APC, recognition of MBP or mitogenic activation of responder T cells elicited high levels of I-A and I-E expression on responders. Acquisition of class II MHC glycoproteins by responders was resistant to the protein synthesis inhibitor cycloheximide, coincided with transfer of a PKH26 lipophilic dye from APC to responders, and resulted in the expression of syngeneic and allogeneic MHC glycoproteins on responders. Unlike rested I-A- T cell clones, rat thymic and splenic T cells expressed readily detectable levels of class II MHC glycoproteins. When preactivated with mitogens, naive T cells acquired APC-derived MHC class II molecules and other membrane-associated proteins when cultured with xenogeneic APC in the absence of Ag. In conclusion, this study provides evidence that APC donate membrane-bound peptide/MHC complexes to Ag-specific T cell responders by a mechanism associated with the induction of tolerance.  (+info)

HLA-E and HLA-G expression on porcine endothelial cells inhibit xenoreactive human NK cells through CD94/NKG2-dependent and -independent pathways. (5/163)

Human NK cells contribute a significant role to host defense as well as xenogeneic cytotoxicity. Previous studies using human 721.221 cell line have shown that peptides derived from the leader sequence of the HLA-G binds and up-regulates the surface expression of HLA-E molecules, which was considered to consequently provide negative signals to human NK cells. However, the direct role of HLA-G in inhibiting human NK cells remains controversial. In this study, we showed that the expression of HLA-G or HLA-E in porcine endothelial cells directly protected sensitive porcine cells from human NK cell-mediated xenogeneic cytotoxicity. Ab blocking assays using F(ab')2 of the HLA class I-specific mAb PA2.6 indicated that the protection was directly mediated by the expression of HLA-G and HLA-E on the porcine cells. The HLA-E-mediated protection was blocked by anti-human CD94 Ab. In addition, the engagement of HLA-E lead to the phosphorylation of the CD94/NKG2 complex and the recruitment of SH2 domain-containing protein phosphatase 1 (SHP-1) to the complex. Therefore, HLA-E protected porcine cells from xenoreactive human NK cells through a CD94/NKG2-dependent pathway. In contrast, HLA-G inhibited human NK cells in the absence of CD94/NKG2 phosphorylation or SHP-1 recruitment, and the inhibition was not blocked by anti-CD94 Ab. Therefore, HLA-G protected porcine cells from human NK cells through a CD94/NKG2-independent pathway. These results demonstrated that both HLA-E and HLA-G could directly inhibit human NK cells in the absence of other endogenous HLA class I molecules. These results also have practical implications in preventing xenograft rejection mediated by human NK cells.  (+info)

Xenotransplantation: the importance of the Galalpha1,3Gal epitope in hyperacute vascular rejection. (6/163)

The transplantation of organs from other species into humans is considered to be a potential solution to the shortage of human donor organs. Organ transplantation from pig to human, however, results in hyperacute rejection, initiated by the binding of human natural antidonor antibody and complement. The major target antigen of this natural antibody is the terminal disaccharide Galalphal,3Gal, which is synthesized by Galbeta1,4GlcNAc alpha1,3-galactosyltransferase. Here we review our current knowledge of this key enzyme. A better understanding of structure, enzyme properties, and expression pattern of alpha1,3-galactosyltransferase has opened up several novel therapeutic approaches to prevent hyperacute vascular rejection. Cloning, and expression in vitro of the corresponding cDNA, has allowed to develop strategies to induce immune tolerance, and deplete or neutralize the natural xenoreactive antibody. Elucidation of the genomic structure has led to the production of transgenic animals that are lacking alpha1,3-galactosyltransferase activity. A detailed knowledge of the enzyme properties has formed the basis of approaches to modify donor organ glycosylation by intracellular competition. Study of the expression pattern of alpha1,3-galactosyltransferase has helped to understand the mechanism of hyperacute rejection in discordant xenotransplantation, and that of complement-mediated, natural immunity against interspecies transmission of retroviruses.  (+info)

Taking lessons from dendritic cells: multiple xenogeneic ligands for leukocyte integrins have the potential to stimulate anti-tumor immunity. (7/163)

Expression of large numbers of different costimulatory integrin ligands (CILs) attributes dendritic cells with an ability to induce primary anti-tumor immune responses. Here, we show that optimized gene transfer of the xenogeneic (human) CILs VCAM-1, MAdCAM-1 and ICAM-1 causes rapid and complete rejection of established mouse EL-4 tumors, and generates prolonged systemic anti-tumor immunity; whereas human E-cadherin weakly slows tumor growth. In each case the immune response was mediated by CD8+ T cells and NK cells, accompanied by augmented tumor-specific cytolytic T cell (CTL) activity involving both the perforin and Fas-ligand pathways. Adoptive transfer of splenocytes from cured mice rapidly cleared established tumors in recipients. The mechanism for CIL-mediated immunity is unknown, but may involve CTL-facilitated tumor lysis, since CTLs were generally twice as efficient at killing CIL-transfected tumor cells than parental tumor cells. Optimized CIL-based gene therapy may provide an approach to complement or replace conventional DC adoptive cell therapy for suppressing tumor growth.  (+info)

Naturally developing memory T cell xenoreactivity to swine antigens in human peripheral blood lymphocytes. (8/163)

Naturally developing xenospecific Abs are well-documented barriers to xenograft transplantation in humans, but whether analogous xenoreactive T cell immunity develops is not known. We used an enzyme-linked immunospot assay to determine the frequency and cytokine profiles of xenoreactive PBLs from a panel of human volunteers. Because naive T cells produce only IL-2 in short term culture, IFN-gamma production by this approach is a measure of a memory immune response. Stimulation of human PBLs or purified T lymphocytes with stimulator cells from inbred swine revealed a high frequency of IFN-gamma producers with 5-fold fewer IL-2 producers. In contrast, lymphocytes obtained from neonatal umbilical cord blood contained swine-specific IL-2 producers but few IFN-gamma producers, which is what one would expect to find with a naive phenotype. Moreover, PBLs from adults with a history of abstention from pork consumption responded to swine cells with a significantly lower frequency of IFN-gamma producers than PBLs from adults with unrestricted diets did, suggesting that pork consumption may result in priming of swine-specific T cell immunity. Our findings provide the first evidence for naturally occurring xenospecific T cell immunity in humans. The detected strength of this memory response suggests that it will present a formidable barrier to transplantation of swine organs.  (+info)

Knock-Out Pigs Press ReleaseKnock-Out Pigs Press Release
PPL THERAPEUTICS PLC. WORLD S FIRST ANNOUNCEMENT OF. CLONED KNOCK-OUT PIGS. Christmas-born pigs are a major step towards successful production of animal organs and cells for human transplantation use. Blacksburg, Virginia: PPL Therapeutics Plc, one of the world s leading biopharmaceutical companies in the application of transgenic technology to the production of human proteins for therapeutic and nutritional applications, is pleased to announce it has produced knock-out piglets which were born as a result of using nuclear transfer (cloning) and PPL s patented gene targeting technology. The five healthy births took place on Christmas Day, December 25, 2001. It has always been the objective of PPL s xenotransplantation program to produce knock-out pigs. In the future, this vital step should enable organs and/or cells from such animals to be transplanted into humans and not be rejected by the human recipient. A knock-out pig has the specific gene that leads to the human immune system rejecting pig ...
https://www.revivicor.com/KOrelease.htm
Experimental evidence showing that no mitotically active female germline progenitors exist in postnatal mouse ovaries | PNASExperimental evidence showing that no mitotically active female germline progenitors exist in postnatal mouse ovaries | PNAS
It has been generally accepted for more than half a century that, in most mammalian species, oocytes cannot renew themselves in postnatal or adult life, and that the number of oocytes is already fixed in fetal or neonatal ovaries. This assumption, however, has been challenged over the past decade. In this study, we have taken an endogenous genetic approach to this question and generated a multiple fluorescent Rosa26rbw/+;Ddx4-Cre germline reporter mouse model for in vivo and in vitro tracing of the development of female germline cell lineage. Through live cell imaging and de novo folliculogenesis experiments, we show that the Ddx4-expressing cells from postnatal mouse ovaries did not enter mitosis, nor did they contribute to oocytes during de novo folliculogenesis. Our results provide evidence that supports the traditional view that no postnatal follicular renewal occurs in mammals, and no mitotically active Ddx4-expressing female germline progenitors exist in postnatal mouse ovaries. ...
https://www.pnas.org/content/early/2012/06/29/1206600109.abstract
Features of N-Glycosylation of Immunoglobulins from Knockout Pig Models | OMICS InternationalFeatures of N-Glycosylation of Immunoglobulins from Knockout Pig Models | OMICS International
For the first time, the N-glycosylation patterns of immunoglobulin G (IgGs) isolated from the serum of two varieties of knockout pigs (lacking N-glycolylneuraminic acid..
https://www.omicsonline.org/open-access/features-of-nglycosylation-of-immunoglobulins-from-knockout-pig-models-2155-9872-1000333.php?aid=78369&view=mobile
Human thrombin and FXa mediate porcine endothelial cell activation; modulation by expression of TFPI-CD4 and hirudin-CD4 fusion...Human thrombin and FXa mediate porcine endothelial cell activation; modulation by expression of TFPI-CD4 and hirudin-CD4 fusion...
Chen, DX, Riesbeck, K, McVey, JH, Kemball-Cook, G, Tuddenham, EGD, Lechler, RI and Dorling, A (2001) Human thrombin and FXa mediate porcine endothelial cell activation; modulation by expression of TFPI-CD4 and hirudin-CD4 fusion proteins ...
http://epubs.surrey.ac.uk/825929/
Rolling adhesion of human NK cells to porcine endothelial cells mainly relies on CD49d-CD106 interactions1Rolling adhesion of human NK cells to porcine endothelial cells mainly relies on CD49d-CD106 interactions1
Acute vascular rejection in pig-to-primate xenotransplantation involves recognition and damage of porcine (po) endothelial cells (EC) by human (hu) leukocytes, probably including natural killer (NK) cells. To study such interactions we analyzed rolling and static adhesion of hu NK cells to po EC. T...
https://insights.ovid.com/transplantation/trans/2002/03/150/rolling-adhesion-human-nk-cells-porcine/23/00007890
Porcine SWC8抗体[MIL3] |Abcam中国|Anti-Porcine SWC8抗体[MIL3]Porcine SWC8抗体[MIL3] |Abcam中国|Anti-Porcine SWC8抗体[MIL3]
Porcine SWC8小鼠单克隆抗体[MIL3](ab34020)可与猪样本反应并经IHC, Flow Cyt实验严格验证，被4篇文献引用。所有产品均提供质保服务，中国75%以上现货。
http://www.abcam.cn/porcine-swc8-antibody-mil3-ab34020.html
Antigenic Heterogeneity of Vascular Endothelium | SpringerLinkAntigenic Heterogeneity of Vascular Endothelium | SpringerLink
In view of the participation of vascular endothelial cells (EC) in a wide range of normal and pathological conditions, phenotypic heterogeneity of EC is an important concept for consideration:...
https://link.springer.com/chapter/10.1007/978-1-4615-3736-6_51
mysql - Bulk-Insert Select performance over individual Inserts - Database Administrators Stack Exchangemysql - Bulk-Insert Select performance over individual Inserts - Database Administrators Stack Exchange
If were talking performance, first approach is better. Here is why:. Whenever new data is inserted into a table, the indexes will also be updated, and physically reordered. In the case on InnoDB, not only are the index files updated, but the data file itself could be reordered, it is clustered based on the primary key. So, re-ordering/updating is an expensive operation, and you want to minimize it.. When you do bulk insert, the indexes are updated at when the statement is finished. i.e. after inserting all rows. On the other hand, when you do individual inserting, physical files are updated after each insert.. The overhead of the last ...
https://dba.stackexchange.com/questions/165449/bulk-insert-select-performance-over-individual-inserts
Interaction of antibody with Forssman antigen in guinea pigs: A mechanism of adaptation to antibody- and complement-mediated...Interaction of antibody with Forssman antigen in guinea pigs: A mechanism of adaptation to antibody- and complement-mediated...
TY - JOUR. T1 - Interaction of antibody with Forssman antigen in guinea pigs. T2 - A mechanism of adaptation to antibody- and complement-mediated injury. AU - Yuzawa, Y.. AU - Brett, J.. AU - Fukatsu, A.. AU - Matsuo, S.. AU - Caldwell, P. R.B.. AU - Niesen, N.. AU - Milgrom, F.. AU - Godman, G.. AU - Stern, D.. AU - Andres, G.. PY - 1995. Y1 - 1995. N2 - Forssman antigen is a glycosphingolipid with antigenic specificity determined by extra-membrane haptenic sugars similar to blood group antigens and antigens that are the main barrier to xenogeneic organ transplantation. Herein, we describe the localization of Forssman antigen in guinea pig lungs and kidneys and the consequences of its interaction with antibodies in vitro and in vivo (Forssman reaction). Exposure of cultured guinea pig aortic endothelial cells to Forssman antibodies induced rapid redistribution of antigen-antibody complexes at the cell surface, followed by shedding that occurred by blebbing of plasma membrane as vesicles or ...
https://pure.fujita-hu.ac.jp/en/publications/interaction-of-antibody-with-forssman-antigen-in-guinea-pigs-a-me
Heterophile | Definition of Heterophile by Merriam-WebsterHeterophile | Definition of Heterophile by Merriam-Webster
Heterophile definition is - of, relating to, or being an antibody circulating in blood serum that is reactive with antigen originating in a different species. How to use heterophile in a sentence.
https://www.merriam-webster.com/dictionary/heterophile
TNF-α promotes human antibody-mediated complement-dependent cytotoxicity of porcine endothelial cells through downregulating...TNF-α promotes human antibody-mediated complement-dependent cytotoxicity of porcine endothelial cells through downregulating...
The major limitation of organ transplantation is the shortage of available organs. Xenotransplantation is considered to be an effective way to resolve the problem. Immune rejection is a major hurdle for the successful survival of pig xenografts in primate recipients. Cytokines play important roles in inflammation and many diseases including allotransplantation, however, their roles in xenotransplantation have been less well investigated. We assessed the role of several cytokines in xenotransplantation using an in vitro model of human antibody-mediated complement-dependent cytotoxicity (CDC). Porcine aortic endothelial cells (PAECs) and porcine iliac endothelial cells (PIECs) were selected as target cells. The complement regulators (CD46, CD55 and CD59) and junction protein genes were assessed by real-time PCR, flow cytometry, or western-blotting assay. Flow cytometry assay was also used to evaluate C3 and C5b-9 deposition, as well as the extent of human IgM and IgG binding to PIECs. Gene silencing was
https://biosignaling.biomedcentral.com/articles/10.1186/s12964-019-0386-7/email/correspondent/c2/new
An antigen-binding cell tumor. Heterophile cold hemagglutinin as a me by H Hiai, H Shisa et al.An antigen-binding cell tumor. Heterophile cold hemagglutinin as a me by H Hiai, H Shisa et al.
Hiai, H; Shisa, H; Nishizuka, Y; and Miyawaki, H, An antigen-binding cell tumor. Heterophile cold hemagglutinin as a membrane-bound receptor on a mouse lymphoma. (1974). Subject Strain Bibliography 1974. 975 ...
https://mouseion.jax.org/ssbb1974/975/
Forssman antigen | definition of Forssman antigen by Medical dictionaryForssman antigen | definition of Forssman antigen by Medical dictionary
Looking for online definition of Forssman antigen in the Medical Dictionary? Forssman antigen explanation free. What is Forssman antigen? Meaning of Forssman antigen medical term. What does Forssman antigen mean?
http://medical-dictionary.thefreedictionary.com/Forssman+antigen
Xenoantibody | definition of xenoantibody by Medical dictionaryXenoantibody | definition of xenoantibody by Medical dictionary
Looking for online definition of xenoantibody in the Medical Dictionary? xenoantibody explanation free. What is xenoantibody? Meaning of xenoantibody medical term. What does xenoantibody mean?
http://medical-dictionary.thefreedictionary.com/xenoantibody
HETEROPHILE ANTIBODY REACTION IN INFECTIOUS MONONUCLEOSIS* | Annals of Internal Medicine | American College of PhysiciansHETEROPHILE ANTIBODY REACTION IN INFECTIOUS MONONUCLEOSIS* | Annals of Internal Medicine | American College of Physicians
The sheep cell agglutination test or heterophile antibody reaction, as it is also called, is a laboratory procedure of considerable value in clinical medicine. In this country one frequently speaks alternately of the Paul-Bunnell test; and in Europe it is often referred to as the Hanganutziu-Deicher reaction. In 1911 Forssman1 recognized the nonspecificity of certain antigen-antibody reactions. The terms heterogenetic, heterophilic, or heterophile are applied to those antibodies that react with an antigen (sheep erythrocytes) which seemingly had nothing to do with their development. One type of heterophile antibody is known as the Forssman antibody, but there are other varieties ...
http://annals.org/aim/article-abstract/673018/heterophile-antibody-reaction-infectious-mononucleosis
Use of molecular modeling and site-directed mutagenesis to define the structural basis for the immune response to carbohydrate...Use of molecular modeling and site-directed mutagenesis to define the structural basis for the immune response to carbohydrate...
TY - JOUR. T1 - Use of molecular modeling and site-directed mutagenesis to define the structural basis for the immune response to carbohydrate xenoantigens. AU - Kearns-Jonker, Mary. AU - Barteneva, Natasha. AU - Mencel, Robert. AU - Hussain, Namath. AU - Shulkin, Irina. AU - Xu, Alan. AU - Yew, Margaret. AU - Cramer, Donald V.. N1 - Copyright: Copyright 2008 Elsevier B.V., All rights reserved.. PY - 2007/3/12. Y1 - 2007/3/12. N2 - Background: Natural antibodies directed at carbohydrates reject porcine xenografts. They are initially expressed in germline configuration and are encoded by a small number of structurally-related germline progenitors. The transplantation of genetically-modified pig organs prevents hyperacute rejection, but delayed graft rejection still occurs, partly due to humoral responses. IgVH genes encoding induced xenoantibodies are predominantly, not exclusively, derived from germline progenitors in the VH3 family. We have previously identified the immunoglobulin heavy chain ...
https://research.nu.edu.kz/en/publications/use-of-molecular-modeling-and-site-directed-mutagenesis-to-define
Antibodies, heterophile synonyms, Antibodies, heterophile antonyms - FreeThesaurus.comAntibodies, heterophile synonyms, Antibodies, heterophile antonyms - FreeThesaurus.com
Synonyms for Antibodies, heterophile in Free Thesaurus. Antonyms for Antibodies, heterophile. 4 synonyms for infectious mononucleosis: glandular fever, kissing disease, mono, mononucleosis. What are synonyms for Antibodies, heterophile?
http://www.freethesaurus.com/Antibodies%2C+heterophile
Most recent papers with the keyword Modifier gene vwf | Read by QxMDMost recent papers with the keyword Modifier gene vwf | Read by QxMD
PURPOSE OF REVIEW: This review describes the most recent progress in xeno lung transplantation (XLTx) to date. It describes the potential mechanisms of early xeno lung graft loss, as well as the latest therapeutic strategies to overcome them. RECENT FINDINGS: Using ex-vivo perfusion models of porcine lungs with human blood, the use of genetically modified pig lungs along with novel pharmaceutical approaches has recently been studied. Strategies that have demonstrated improved lung survival include the knockout of known xenoantigens (GalTKO and N-glycolylneuraminic acid-KO), genes that regulate complement activation (hCD46 and hCD55), as well as the inflammation/coagulation cascade (human leukocyte antigen-E, human thrombomodulin, human endothelial protein C receptor, hCD47, hCD39, hCD73 and heme oxygenase-1 ...
https://www.readbyqxmd.com/keyword/119050
New Century Labs | Heterophile Mono ScreenNew Century Labs | Heterophile Mono Screen
Heterophile antibodies, in patients with infectious mononucleosis, may be present as early as the fourth day of illness, and by the twenty-first day of illness, 90% of patients will exhibit a positive test. The Epstein-Barr virus causes infectious mononucleosis.
https://newcenturylabs.com/product/heterophile-mono-screen
Efficiency of porcine endothelial cell infection with human cytomegalovirus depends on both virus tropism and endothelial cell...Efficiency of porcine endothelial cell infection with human cytomegalovirus depends on both virus tropism and endothelial cell...
TrendTerms displays relevant terms of the abstract of this publication and related documents on a map. The terms and their relations were extracted from ZORA using word statistics. Their timelines are taken from ZORA as well. The bubble size of a term is proportional to the number of documents where the term occurs. Red, orange, yellow and green colors are used for terms that occur in the current document; red indicates high interlinkedness of a term with other terms, orange, yellow and green decreasing interlinkedness. Blue is used for terms that have a relation with the terms in this document, but occur in other documents ...
https://www.zora.uzh.ch/id/eprint/41526/
key:work eclrrhj6wzbvrcqwzudcwwxv4e - Internet Archive Scholarkey:work eclrrhj6wzbvrcqwzudcwwxv4e - Internet Archive Scholar
e α1,3-galactose (Gal) antigens, boosted by exposure to porcine red blood cells (PRBC) and the depletion of anti-Gal antibodies with GAS914. Production of anti-non-Gal antibodies correlated on day 10 with an augmented IgM and IgG antibody reactivity to L35 (porcine lymphoblastic cells). On days 20 and 30 there was an increased binding of IgG to AOC-40 (porcine endothelial cells), which paralleled an IgG antibody binding to HMEC-1 (human microvascular endothelial cells). These antibodies caused the apoptosis of AOC-40 and HMEC-1 cells through two different pathways, with and without DNA fragmentation, respectively. Western blotting of anti-non-Gal antibodies showed the increased intensity of several protein bands in AOC-40 and HMEC-1 lysates, and the transient detection of a few new bands, compared to samples before PRBC injection. Treatment with cyclophosphamide in one animal led to the virtual disappearance of anti-non-Gal antibody binding to AOC-40 and HMEC-1 proteins without modifying the ...
https://scholar.archive.org/search?q=key:work_eclrrhj6wzbvrcqwzudcwwxv4e
Sandwhich ELISA using pig serum - ELISASandwhich ELISA using pig serum - ELISA
Heterophile abs. Abs in the samples against abs in the assay. Only way to remove is review your data and locate high false positives. Add into your sample buffer non-specific ab (IgG) of the species used as capture and detector in your system and check with the false positive. ,,use this buffer and pre-incubate the samples to bind up the heterophile ab,, Once the false positive is reduced or eliminated test a select few of your samples. Hopefully, the samples will line up according to the conentration you expect ...
http://www.protocol-online.org/biology-forums/posts/39615.html
Four Ways Oryx and Crake Predicted the Future - Science FridayFour Ways Oryx and Crake Predicted the Future - Science Friday
The goal of the pigoon project was to grow an assortment of foolproof human-tissue organs in a transgenic knockout pig host-organs that would transplant smoothly and avoid rejection, but would also be able to fend off attacks by opportunistic microbes and viruses, of which there were more strains every year. A rapid maturity gene was spliced in so the pigoon kidneys and livers and hearts would be ready sooner, and now they were perfecting a pigoon that could grow five or six kidneys at a time. Such a host animal could be reaped of its extra kidneys; then, rather than be destroyed, it could keep on living and grow more organs, much as a lobster could grow another claw to replace a missing one. That would be less wasteful, as it took a lot of food and care to grow a pigoon.-from Oryx and Crake, chapter 2. The prospect of animals growing human organs for harvesting just got a bit of a reality boost: A couple weeks ago, the National Institutes of Health proposed changes to federal guidelines for ...
https://www.sciencefriday.com/articles/four-ways-oryx-and-crake-predicted-the-future/
Engraftment and development of xenogeneic cells in normal mammals having reconstituted hematopoietic deficient immune...
Non-human chimeric mammals are created from a mammal having hematopoietic cells replaced with hematopoietic cells from a hematopoietic deficient mammal donor, and optionally in which xenogeneic cells and/or tissue are engrafted. The xenogeneic, preferably human, cells or tissue may be hematopoietic cells, in which case the chimeric mammal can produce xenogeneic B and/or T cells, and can be used as a source of mammalian, preferably human, monoclonal antibodies and/or T cells. Alternatively, the xenogeneic cells or tissue may be non-hematopoietic, such as normal or pathological cells or tissue, which can form a stable transplant in the chimeric mammal and thus can be used as an animal model of various pathologies or to test therapeutic or diagnostic agents or modalities.
http://www.patentgenius.com/patent/5709843.html
Gentaur Molecular :ICL \ anti-Pig IgG h+l Unconjugated A.P. Host Goat \ GGHL-5AGentaur Molecular :ICL \ anti-Pig IgG h+l Unconjugated A.P. Host Goat \ GGHL-5A
Gentaur molecular products has all kinds of products like :search , ICL \ anti-Pig IgG h+l Unconjugated A.P. Host Goat \ GGHL-5A for more molecular products just contact us
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Rabbit Anti-Pig AP (IgG H&L) (ab6778) | AbcamRabbit Anti-Pig AP (IgG H&L) (ab6778) | Abcam
Anti-Pig Alkaline Phosphatase validated for WB, ELISA, IHC-P, IHC-Fr, IM, Dot, ICC/IF. Other Alkaline Phosphatase secondaries available.
http://www.abcam.com/rabbit-pig-igg-hl-alkaline-phosphatase-ab6778.html
SWISS-MODEL Template Library | 6s04.1
SWISS-MODEL Template Library (SMTL) entry for 6s04.1. Crystal structure of an inverting family GH156 exosialidase from uncultured bacterium pG7 in complex with N-glycolylneuraminic acid
https://swissmodel.expasy.org/templates/6s04
Gmail Resource: Gmail Drive 1.04Gmail Resource: Gmail Drive 1.04
GMail Drive is a Shell Namespace Extension that creates a virtual filesystem around your Google GMail account, allowing you to use GMail as a storage medium. GMail Drive creates a virtual filesystem on top of your Google GMail account and enables you to save and retrieve files stored on your GMail account directly from inside Windows Explorer. GMail Drive literally adds a new drive to your computer under the My Computer folder, where you can create new folders, copy and dragndrop files to ...
http://gmailresource.blogspot.com/2004/12/gmail-drive-104.html
Heterophile antibody test | Article about heterophile antibody test by The Free DictionaryHeterophile antibody test | Article about heterophile antibody test by The Free Dictionary
Looking for heterophile antibody test? Find out information about heterophile antibody test. heterophile agglutination test Explanation of heterophile antibody test
http://encyclopedia2.thefreedictionary.com/heterophile+antibody+test
Mechanism of leflunomide-induced prevention of xenoantibody formation and xenograft rejection in the hamster to rat heart...Mechanism of leflunomide-induced prevention of xenoantibody formation and xenograft rejection in the hamster to rat heart...
Mechanism of leflunomide-induced prevention of xenoantibody formation and xenograft rejection in the hamster to rat heart transplantation model
https://pubmed.ncbi.nlm.nih.gov/7879012/?dopt=Abstract
Sequence DetailSequence Detail
J:188842 Bergfeld AK, et al., Metabolism of vertebrate amino sugars with N-glycolyl groups: elucidating the intracellular fate of the non-human sialic acid N-glycolylneuraminic acid. J Biol Chem. 2012 Aug 17;287(34):28865-81 ...
http://www.informatics.jax.org/sequence/Q9DCJ9
Primary Porcine Cells | Swine | Pig | Cell ApplicationsPrimary Porcine Cells | Swine | Pig | Cell Applications
Our new headquarters is located at 6455 Weathers Place, San Diego, CA 92121.. Please update our contact info in your address book.. ...
https://www.cellapplications.com/porcine
Xenotransplantation | 2018(07) articlesXenotransplantation | 2018(07) articles
xenotransplantation - Read articles from Issue 2018(07). Read article PDFs using your inistitutions subscriptions with no additional login.
https://insights.ovid.com/xenotransplantation/xeno/2018/07/000
Goat Anti-Pig IgG Polyclonal Antibody (Unconjugated) | VWRGoat Anti-Pig IgG Polyclonal Antibody (Unconjugated) | VWR
Special note about Community Feature Content. Any content and/or opinions uploaded, expressed or submitted through any Community Feature or any other publicly available section of the Web Site (including password-protected areas), and all articles and responses to questions, other than the content explicitly authorized by the Company, are solely the opinions and responsibility of the person or entity submitting them and do not necessarily reflect the opinions of the Company. By way of example, any recommended or suggested use of products or services available from the Company that is posted through a Community Feature is not a sign of approval or recommendation by the Company. If you choose to follow any such recommendation you do so at your own risk.. Links to Third Party Sites. The Web Site may contain links to other websites on the internet. The Company is not responsible for the content, products, services or practices of any third party websites, including without limitation sites linked to ...
https://us.vwr.com/store/product/17060162/goat-anti-pig-igg-polyclonal-antibody-unconjugated
Erratum: Absence of Gal epitope prolongs survival of swine lungs in an ex vivo model of hyperacute rejection ...
Fingerprint Dive into the research topics of Erratum: Absence of Gal epitope prolongs survival of swine lungs in an ex vivo model of hyperacute rejection (Xenotransplantation (2011) DOI: 10.1111/j.1399-3089.2011.00633.x). Together they form a unique fingerprint. ...
https://augusta.pure.elsevier.com/en/publications/erratum-absence-of-gal-epitope-prolongs-survival-of-swine-lungs-i/fingerprints/
Glycotope Receives Regulatory Approval For Glycoexpress(TM) Technology And Initiates First Clinical Trial With Lead Antibody GT...Glycotope Receives Regulatory Approval For Glycoexpress(TM) Technology And Initiates First Clinical Trial With Lead Antibody GT...
Glycotope Receives Regulatory Approval For Glycoexpress(TM) Technology And Initiates First Clinical Trial With Lead Antibody GT-MAB 2.5-GEX - read this article along with other careers information, tips and advice on BioSpace
https://www.biospace.com/article/releases/glycotope-receives-regulatory-approval-for-glycoexpress-tm-technology-and-initiates-first-clinical-trial-with-lead-antibody-gt-mab-2-5-gex-/
Genetic modification of alpha-Gal expression in xenogeneic endothelial cells yields a complex immunological response - Leach LabGenetic modification of alpha-Gal expression in xenogeneic endothelial cells yields a complex immunological response - Leach Lab
https://jleachlab.org/wp-content/uploads/blank.png 0 0 Academic Web Pages https://jleachlab.org/wp-content/uploads/blank.png Academic Web Pages2001-01-01 00:00:002001-01-01 00:00:00Genetic modification of alpha-Gal expression in xenogeneic endothelial cells yields a complex immunological response ...
https://jleachlab.org/publication/genetic-modification-of-alpha-gal-expression-in-xenogeneic-endothelial-cells-yields-a-complex-immunological-response/
What Is Xenotransplantation?
Xenotransplantation is the transplanting of animal tissues and organs into a human, and is being studied as a substitute for human organ donors.
http://www.essortment.com/xenotransplantation-62361/
Xenotransplantation by Kaitlin Ball on PreziXenotransplantation by Kaitlin Ball on Prezi
How its done: Doctors take an organ that was donated by an animal source (most commonly pigs are used in this process) and surgically implant it into a human recipient that needs the new organs ...
https://prezi.com/rsg6svtvpth1/xenotransplantation/
Prezcobix Advanced Patient Information - Drugs.comPrezcobix Advanced Patient Information - Drugs.com
Detailed drug Information for Prezcobix. Includes common brand names, drug descriptions, warnings, side effects and dosing information.
https://www.drugs.com/cons/prezcobix.html
anti-Porcine circovirus type 2 Capsid antibody | GeneTexanti-Porcine circovirus type 2 Capsid antibody | GeneTex
Porcine circovirus type 2 Capsid antibody for IHC, IHC-P, WB. Anti-Porcine circovirus type 2 Capsid pAb (GTX128120) is tested in Porcine circovirus type 2 samples. 100% Ab-Assurance.
http://www.genetex.com/Porcine-circovirus-type-2-Capsid-antibody-GTX128120.html
Complete chemoenzymatic synthesis of the Forssman antigen using novel glycosyltransferases identified in Campylobacter jejuni...Complete chemoenzymatic synthesis of the Forssman antigen using novel glycosyltransferases identified in Campylobacter jejuni...
Complete chemoenzymatic synthesis of the Forssman antigen using novel glycosyltransferases identified in Campylobacter jejuni and Pasteurella multocida
http://nparc.nrc-cnrc.gc.ca/eng/view/object/?id=a9c45c83-8be0-46a4-9b57-297e7e95911e
Immunological responses in mice to full-thickness corneal grafts engineered from porcine collagenImmunological responses in mice to full-thickness corneal grafts engineered from porcine collagen
Tissue-engineered (TE) corneas were fabricated from porcine collagen cross-linked with 1-ethyl-3-(3-dimethyl aminoproplyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS), and were transplanted into BALB/c mice orthotopically using a full-thickness penetrating keratoplasty (PKP) procedure. The biocompatibility was evaluated by assessing both local and systemic immune responses. Myeloid cells including granulocytes and macrophages were the main infiltrating cells in recipient cornea and in retro-TE corneal membrane which developed 7-10 days post surgery. Sodium citrate was found to be effective in reducing fibrin accumulation in anterior chamber post grafting at early time points, but it did not prevent formation of the retro-TE corneal membrane. No significant T cell activation was observed in the submandibular draining lymph nodes (SMDLN) by flow cytometry. Anti-porcine type I collagen IgG antibodies were detected in the serum of grafted mice from 2 weeks post grafting and the concentration of ...
http://liu.diva-portal.org/smash/record.jsf?pid=diva2:441031
Elimination of N-Glycolylneuraminic Acid From Animal Products For Human Use - Patent applicationElimination of N-Glycolylneuraminic Acid From Animal Products For Human Use - Patent application
0113] Free Neu5Gc can be taken up by human epithelial cells from an exogenous source and incorporated into different subcellular fractions. Evidence was presented suggesting that the small amounts of Neu5Gc found in some human tissues originated from dietary sources and showed that human Caco-2 cells (human epithelial cells from a primary colon carcinoma) in culture could metabolically incorporate free Neu5Gc, as determined by a Western blot of a total homogenate, using and anti-Neu5Gc antibody. Increasing incorporation of Neu5Gc was found in the total homogenate fraction of the cells over time, with the highest level reached after incubation with 3 mM Neu5Gc for 3 days. Moreover, Western blotting with an anti Neu5Gc antibody demonstrated metabolic incorporation of Neu5Gc into glycoproteins of these cells. The partitioning of the exogenous Neu5Gc into different subcellular fractions of these cells has now been studied. Prior to feeding, Caco-2 cells were split and cultured in human serum instead ...
http://www.patentsencyclopedia.com/app/20140044798
Heterophile Antibody (Infectious Mononucleosis) by Latex Agglutination with Reflex to Titer | ARUP Lab Test DirectoryHeterophile Antibody (Infectious Mononucleosis) by Latex Agglutination with Reflex to Titer | ARUP Lab Test Directory
Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map. ...
https://ltd.aruplab.com/Tests/Pub/2007697
Goat anti-Pig IgG-heavy and light chain cross adsorbed Antibody Affinity Purified - A100-205A - 0.5 mg - Bethyl Laboratories,...Goat anti-Pig IgG-heavy and light chain cross adsorbed Antibody Affinity Purified - A100-205A - 0.5 mg - Bethyl Laboratories,...
Goat anti-Pig IgG-heavy and light chain cross adsorbed Antibody Affinity Purified - 0.5 mg - Bethyl Laboratories, Inc. - antibodies
https://www.bioscience.co.uk/product~68887
SureStep™ Mono TestSureStep™ Mono Test
SureStep™ Mono Test,A rapid test for the visual, qualitative detection of heterophile antibodies specific to infectious mononucleosis in human serum, plasma or whole blood specimens. This test is intended as an aid in the diagnosis of infectious mononucleosis in patients with characteristic clinical symptoms.,medicine,medical supply,medical supplies,medical product
http://www.bio-medicine.org/medicine-products/SureStep-26trade-3B-Mono-Test-9898-1/
QuickVue+ Mononucleosis Test | QuidelQuickVue+ Mononucleosis Test | Quidel
The QuickVue+ Mononucleosis Test detects IgM heterophile antibodies in acute phase infections using serum, plasma or whole blood.
https://www.quidel.com/immunoassays/rapid-mono-tests/quickvue-mononucleosis-test
Xenotransplantation - the transplant of organs or tissues between different species has become a major issue within the pastXenotransplantation - the transplant of organs or tissues between different species has become a major issue within the past
ISIS Sustainable Science Audit #2. August 2000. Xenotransplantation How Bad Science and Big Business Put the World at Risk from Viral Pandemics. Dr. Mae-Wan Ho. Director. Institute of Science in Society. PO Box 32097. London NW1 0XR. UK. Summary. Xenotransplantation - the transplant of animal organs into human beings - is a multi-billion dollar business venture built on the anticipated sale of patented techniques and organs, as well as drugs to overcome organ-rejection (1). It has received strong criticism and opposition from scientists warning of risks of new viruses crossing from animal organs to human subjects and from there to infect the population at large. Our regulators, however, are adopting a permissive attitude for clinical trials to go ahead. Scientific reports of virus crossing from pig to human cells (2), and of viral infections in humans subjects transplanted with baboon livers (3), are being ignored or dismissed, while inconclusive, widely faulted papers are taken as evidence that ...
https://www.iatp.org/files/Xenotransplantation_How_Bad_Science_and_Big_Bu.htm
Jens Krause | IGBJens Krause | IGB
J. Krause; J. E. Herbert-Read; F. Seebacher; P. Domenici; A. D. M. Wilson; S. Marras; M. B. S. Svendsen; D. Strömborn; J. F. Steffensen; S. Krause; P. E. Viblanc; P. Couillaud; P. Bach; P. S. Sabarros; P. Zaslansky; R. H. J. M. ...
http://www.igb-berlin.de/profile/jens-krause
Heterophile antigenHeterophile antigen
Heterophile antigens are antigens of similar nature, if not identical, that are present in different tissues in different ... Example: Forssman antigen, cross reacting microbial antigen so antibodies to these antigens produced by one species cross react ... but the hetereophile antigen is shared by different species. Other heterophile antigens are responsible for some diagnostic ... "Studies on heterophile antigen and antibody". Journal of Pathology. 24 (2): 217-240. doi:10.1002/path.1700240214. v t e v t e ( ...
https://en.wikipedia.org/wiki/Heterophile_antigen
History and naming of human leukocyte antigensHistory and naming of human leukocyte antigens
Rapaport FT, Kano K, Milgrom F (1968). "Heterophile antibodies in human transplantation". J. Clin. Invest. 47 (3): 633-42. doi: ... The Hu-1 antigens were renamed the Human-lymphoid (HL) allo-antigens (HL-As). Allo-antigen comes from the observation that a ... Human leukocyte antigens (HLA) began as a list of antigens identified as a result of transplant rejection. The antigens were ... As a result, scientists were able to identify a few antigens. At first the first antigens were called the Hu-1 antigens and ...
https://en.wikipedia.org/wiki/History_and_naming_of_human_leukocyte_antigens
Heterophile antibodyHeterophile antibody
Heterophile antibodies are antibodies induced by external antigens (heterophile antigens). Some cross-react with self-antigens ... Heterophile antigen An immunoassay is a biochemical test, frequently used in medical diagnostic testing, that measures the ... These are considered heterophile antibodies. In clinical diagnosis, the heterophile antibody test specifically refers to a ... Heterophile antibodies can cause significant interference in any immunoassay. The presence of a heterophile antibody is ...
https://en.wikipedia.org/wiki/Heterophile_antibody
Infectious mononucleosisInfectious mononucleosis
Elgh, F; Linderholm, M (1996). "Evaluation of six commercially available kits using purified heterophile antigen for the rapid ... Early antigen (EA) Anti-EA IgG appears in the acute phase of illness and disappears after 3 to 6 months. It is associated with ... EBV nuclear antigen (EBNA) Antibody to EBNA slowly appears 2 to 4 months after the onset of symptoms and persists for the rest ... The heterophile antibody test is a screening test that gives results within a day, but has significantly less than full ...
https://en.wikipedia.org/wiki/Infectious_mononucleosis
John ForssmanJohn Forssman
He is known for discovery of the "Forssman antigen", defined as a glycolipid heterophile antigen found on tissue cells of many ... His name is also associated with the following two terms: "Forssman antibody" (heterophile antibody) and "Forssman reaction", ... also referred to as a "Forssman antigen-antibody reaction". Über die Ursachen, welche die Wachsthumsrichtung der peripheren ...
https://en.wikipedia.org/wiki/John_Forssman
Cross-reactivityCross-reactivity
In immunology, cross-reactivity has a more narrow meaning of the reaction between an antibody and an antigen that differs from ... An example of helpful cross-reactivity is in heterophile antibody tests, which detect Epstein-Barr virus using antibodies with ... There can be cross-reactivity between the immune system and the antigens of two different pathogens, or between one pathogen ... For example, the tetanus toxin is a single protein macromolecular antigen but will stimulate many immune responses due to the ...
https://en.wikipedia.org/wiki/Cross-reactivity
Heterophile antibody testHeterophile antibody test
Heterophile also can mean that it is an antibody that reacts with antigens other than the antigen that stimulated it (an ... Elgh, F; Linderholm, M (1996). "Evaluation of six commercially available kits using purified heterophile antigen for the rapid ... It has the following characteristics: VCAs (Viral Capsid Antigen) of the IgM class, antibodies to EBV early antigen (anti-EA), ... An elevated heterophile antibody level may persist up to 1 year. The test is usually performed using commercially available ...
https://en.wikipedia.org/wiki/Heterophile_antibody_test
Index of immunology articlesIndex of immunology articles
Herd immunity Heterophile Heterophile antigen Heterosubtypic immunity Histamine Histocompatibility Human leukocyte antigen ... opsonization Antibody-dependent cell-mediated cytotoxicity Antibody-dependent enhancement Antigen Antigen presentation Antigen ... Fc receptor Fc receptor-like molecule FcεRI Fibroblast-like synoviocyte FITkit FluoroSpot Forssman antigen Fragment antigen- ... DAMPs Danger model Defensin Degranulation Dendritic cell Dextran 1 Dispanin Dog leukocyte antigen Drug resistance Duffy antigen ...
https://en.wikipedia.org/wiki/Index_of_immunology_articles
List of MeSH codes (D12.776)List of MeSH codes (D12.776)
... antigen, b-cell MeSH D12.776.377.715.548.950.500 - antigens, cd79 MeSH D12.776.377.715.647.100 - alpha-macroglobulins See List ... heterophile MeSH D12.776.377.715.548.114.224 - antibodies, monoclonal MeSH D12.776.377.715.548.114.224.570 - muromonab-cd3 MeSH ... antigen-antibody complex MeSH D12.776.377.715.548.114.301 - antitoxins MeSH D12.776.377.715.548.114.301.138 - antivenins MeSH ... antigens, polyomavirus transforming MeSH D12.776.624.664.520.420 - papillomavirus e7 proteins MeSH D12.776.624.664.520.750 - ...
https://en.wikipedia.org/wiki/List_of_MeSH_codes_(D12.776)
List of MeSH codes (D12.776.124)List of MeSH codes (D12.776.124)
... antigen, b-cell MeSH D12.776.124.486.485.950.500 - antigens, cd79 MeSH D12.776.124.790.106.050 - alpha 1-antichymotrypsin MeSH ... heterophile MeSH D12.776.124.486.485.114.224 - antibodies, monoclonal MeSH D12.776.124.486.485.114.224.570 - muromonab-cd3 MeSH ... antigens, cd46 MeSH D12.776.124.486.274.920.250 - complement c1 inactivator proteins MeSH D12.776.124.486.274.920.250.500 - ... antigen-antibody complex MeSH D12.776.124.486.485.114.301 - antitoxins MeSH D12.776.124.486.485.114.301.138 - antivenins MeSH ...
https://en.wikipedia.org/wiki/List_of_MeSH_codes_(D12.776.124)
PharyngitisPharyngitis
The heterophile test can be used if this is suspected. Herpes simplex virus can cause multiple mouth ulcers. Measles Common ... Otherwise, a rapid antigen detection test (RADT) or throat swab is recommended. Other conditions that can produce similar ...
https://en.wikipedia.org/wiki/Pharyngitis
Pregnancy testPregnancy test
Direct measurement of antigens, such as hCG, was made possible after the invention of the radioimmunoassay in 1959. ... heterophile antibodies, enterocystoplasties, gestational trophoblastic diseases (GTD), and gestational trophoblastic neoplasms ...
https://en.wikipedia.org/wiki/Pregnancy_test
Fatal Respiratory Diphtheria in a U.S. Traveler to Haiti --- Pennsylvania, 2003Fatal Respiratory Diphtheria in a U.S. Traveler to Haiti --- Pennsylvania, 2003
A rapid test for group A streptococcal antigens and a test for heterophile agglutinins were negative; he received oral ...
https://www.cdc.gov/mmwr/preview/mmwrhtml/mm5253a3.htm
Epstein-Barr Virus (EBV) Infectious Mononucleosis (Mono) Workup: Approach Considerations, Laboratory Studies, Imaging StudiesEpstein-Barr Virus (EBV) Infectious Mononucleosis (Mono) Workup: Approach Considerations, Laboratory Studies, Imaging Studies
These heterophile antibodies react to antigens from animal RBCs.. * Sheep RBCs agglutinate in the presence of heterophile ... Viral capsid antigen (VCA) - Anti-VCA IgM and anti-VCA IgG antibodies directed against EBV viral capsid antigen are present at ... Heterophile antibodies. Since heterophile antibodies typically return quicker than EBV specific antibodies, it is the test of ... Patients who remain heterophile negative after 6 weeks with a mononucleosis illness should be considered as having heterophile- ...
https://emedicine.medscape.com/article/222040-workup
NHANES 2011-2012: Tuberculosis - Quantiferon In Gold Data Documentation, Codebook, and Frequencies
LBXTBA - TB Antigen result. Variable Name: LBXTBA. SAS Label: TB Antigen result. English Text: TB Antigen result. Target: Both ... The effect of heterophile antibodies in the QuantiFERON®-TB Gold IT ELISA is minimized by the addition of normal mouse serum to ... LBXTBA (TB antigen value) minus LBXTBN (Nil) value must be =, 0.35 IU gamma interferon (IF)/ml AND LBXTBA (TB antigen value) ... TB Antigen tube and a Mitogen tube (positive control). The tubes are shaken to mix antigen with the whole blood and incubated ...
https://wwwn.cdc.gov/Nchs/Nhanes/2011-2012/TB_G.htm
Law Document English View | Ontario.caLaw Document English View | Ontario.ca
Hepatitis Associated Antigen or Antibody Immunoassay (e.g. hepatitis B surface antigen or antibody, hepatitis B anticore ... Heterophile Antibodies - screen (slide or single tube) - with or without absorption.. 70. Virus antibodies - hemagglutination ... Prostate Specific Antigen (PSA), Total.. O. Reg. 46/98, s. 2; O. Reg. 62/02, s. 1; O. Reg. 239/04, s. 6; O. Reg. 71/07, s. 2; O ... 5. That a Hepatitis B surface antigen test not be performed if the person requesting the test indicates that the test is for a ...
https://www.ontario.ca/laws/regulation/900682/v20
Women in Poultry ScienceWomen in Poultry Science
Shorb developed a heterophile antigen; it was widely used to treat pneumonia before the use of sulfa drugs. ...
https://cals.ncsu.edu/prestage-department-of-poultry-science/about/women-in-poultry-science/
Infectious Mononucleosis (IM) in Emergency Medicine: Background, Pathophysiology, EpidemiologyInfectious Mononucleosis (IM) in Emergency Medicine: Background, Pathophysiology, Epidemiology
... heterophile antibodies). Antibodies against specific EBV antigens and a number of autoantibodies also are produced. ... In 1932, the association between the disease syndrome and a positive heterophile antibody test was recognized. It was not until ...
http://emedicine.medscape.com/article/784513-overview
Pathogen Safety Data Sheets: Infectious Substances - Epstein-Barr virus - Canada.caPathogen Safety Data Sheets: Infectious Substances - Epstein-Barr virus - Canada.ca
Tests for heterophile antibodies in mononucleosis, antibodies against viral capsid antigen (VCA), antibodies to EBV nuclear ... Direct detection of the viral antigen can be done by staining for EBNA 1 using anti complement immunofluorescence; viral RNA or ... antigen (EBNA) can also be usedFootnote 1Footnote 2Footnote 4. ...
https://www.canada.ca/en/public-health/services/laboratory-biosafety-biosecurity/pathogen-safety-data-sheets-risk-assessment/epstein-barr-virus.html
Hypertriglyceridemia during infectious mononucleosis | Research SquareHypertriglyceridemia during infectious mononucleosis | Research Square
The serum heterophile antibody test was negative; however, the IgM against the viral capsid antigen (VCA) of EBV was positive. ... followed by antibodies against EBV nuclear antigen (EBNA), with a progressive disappearance of IgM against VCA, thereby ... was considered when the usual clinical syndrome was accompanied by positive IgM antibodies against the viral capsid antigen of ...
https://www.researchsquare.com/article/rs-915947/v1
TC83 replicon vectored vaccine provides protection against Junin virus in guinea pigs - Fingerprint - UTMB Health Research...TC83 replicon vectored vaccine provides protection against Junin virus in guinea pigs - Fingerprint - UTMB Health Research...
Heterophile Antigens 21% * Investigational Drugs 20% * Attenuated Vaccines 18% * Argentina 17% * Viral Antigens 17% ...
https://researchexperts.utmb.edu/en/publications/tc83-replicon-vectored-vaccine-provides-protection-against-junin-/fingerprints/
WHO EMRO | Trend and seroprevalence of Epstein-Barr virus in Bahrain: 2001-2015 | Volume 23, issue 12 | EMHJ volume 23, 2017
... for heterophile antibodies), and enzyme immune assay for detection of early antigens, the viral capsid antigens (VCA) and the ... The presence or absence of EBV viral capsid ‎antigen (VCA) IgG, VCA IgM and EBV nuclear antigen (EBNA) IgG antibodies was ... EBV nuclear antigen (EBNA). Full automation of EBV serological diagnosis is important for routine diagnostic laboratories (6). ...
https://www.emro.who.int/fr/emhj-volume-23-2017/volume-23-issue-12/trend-and-seroprevalence-of-epsteinbarr-virus-in-bahrain-20012015.html
Epstein-Barr Virus - EBV | Choose the Right TestEpstein-Barr Virus - EBV | Choose the Right Test
... and Epstein-Barr nuclear antigen (EBNA) can be used to follow up on negative or positive heterophile antibody test results. ... Heterophile Antibody Test. The heterophile antibody test (Monospot) is a standard diagnostic test for EBV IM in patients with ... Components: EBV antibody to VCA, IgG and IgM; EBV antibody to nuclear antigen, IgG; and EBV antibody to early D antigen (EA-D ... the heterophile antibody test should not be used in children younger than 5 years, most of whom do not produce the heterophile ...
https://arupconsult.com/content/epstein-barr-virus
Infectious Mononucleosis/Reactive Lymphocytes - A Laboratory Guide to Clinical HematologyInfectious Mononucleosis/Reactive Lymphocytes - A Laboratory Guide to Clinical Hematology
Positive heterophile antibody*. Positive EBV specific antigen and antibody (ELISA)*. Elevated C-reactive protein (CRP) ... Positivity for antigen or antibody varies depending on the date of testing. Some antigen or antibodies may appear only after a ...
https://openeducationalberta.ca/mlsci/chapter/infectious-mononucleosis/
DeCSDeCS
Heterologous Antigens Heterophil Antigens Heterophile Antigen Heterophile Antigens Paul Bunnell Antigens Paul-Bunnell Antigens ... Heterologous Antigens. Heterophil Antigens. Heterophile Antigen. Heterophile Antigens. Paul Bunnell Antigens. Paul-Bunnell ... Antigens, Heterophile Entry term(s). Antigens, H-D Antigens, Hanganutziu-Deicher Antigens, Heterogenetic Antigens, Heterologous ... Antigens, Heterophil Antigens, Paul-Bunnell Antigens, Xenogeneic Antigens, Xenogenic H D Antigens H-D Antigens Hanganutziu ...
https://decs.bvsalud.org/en/ths/resource/?id=28230
IMSEAR at SEARO: Search
Pediatric Puzzler: Unremitting feverPediatric Puzzler: Unremitting fever
You opt to wait for results of viral culture and rapid viral antigen testing from a nasopharyngeal swab and to reexamine the ... Could this be Epstein-Barr virus (EBV) infection? A Monospot (rapid heterophile antibody) test is negative, but the test can be ... Rapid viral antigen testing is negative for respiratory syncytial virus, adenovirus, parainfluenza, and influenza. The rectal ...
https://www.contemporarypediatrics.com/view/pediatric-puzzler-unremitting-fever
Activation of natural killer cells and macrophages by porcine endothelial cells augments specific T-cell xenoresponse -...Activation of natural killer cells and macrophages by porcine endothelial cells augments specific T-cell xenoresponse -...
Heterophile Antigens 22% * T-Lymphoid Precursor Cells 21% * T-Cell Receptor Genes 20% ...
https://pure.fujita-hu.ac.jp/en/publications/activation-of-natural-killer-cells-and-macrophages-by-porcine-end/fingerprints/
DeCS 2011 - December 22, 2011 versionDeCS 2011 - December 22, 2011 version
Xenoantibodies use Antibodies, Heterophile Xenoantigens use Antigens, Heterophile Xenobiotics Xenodiagnosis Xenograft use ...
https://decs.bvs.br/I/DeCS2011_Alfab-X.htm
Upper Respiratory Tract Infection: Practice Essentials, Background, PathophysiologyUpper Respiratory Tract Infection: Practice Essentials, Background, Pathophysiology
... positive rapid antigen detection tests do not necessitate a backup culture) ... Mononucleosis: Heterophile antibody testing (eg, Monospot). * Herpes simplex virus infection: Cell culture or polymerase chain ...
https://www.medscape.com/answers/302460-86850/what-are-the-potential-complications-of-pertussis-in-hospitalized-infants
Services - McCone County Health CenterMcCone County Health CenterServices - McCone County Health CenterMcCone County Health Center
35.00 H PYLORI ANTIGEN FECES $60.00 HEPATITIS B SURFACE AG EIA $69.00 HBSAG NEUTRALIZATION CONFIRM $144.00 INFLUENZA A/B AG EIA ... 125.25 HETEROPHILE ANTIBODY SCREEN $24.00 IMMUNOASSAY INFECTIOUS AGENT $158.50 IMMUNOFIX E-PHORESIS SERUM $124.00 INHIBIN A $ ... 148.00 PROTEIN C ANTIGEN PLASMA $275.00 PROTIEN S ACTIVITY $182.75 D-DIMER $150.00 LAP SCORE $63.00 PROTHROMBIN TIME $50.50 ... 385.00 COMPLIMENT ANTIGEN C4 $48.00 ESTRASE INHIBITOR FUNCTION C1 $62.00 CCP ANTIBODY $66.50 DNA ANTIBODY NATIVE $77.00 NUCEAR ...
https://mcconehealth.org/services/
Interference Evaluation Heterophile, Beta-Human Chorionic Gonadotropin, Serum - Mayo Clinic Laboratories | Pediatric Catalog
Some patients, due to exposure to animal antigens, have developed antibodies that interfere with immunoassay testing. These ... Heterophile antibody evaluation consists of pretreatment with commercial heterophile antibody blocking reagents, testing on an ... Test ID IEHCG Interference Evaluation Heterophile, Beta-Human Chorionic Gonadotropin, Serum Ordering Guidance. If "HCG Total OB ... The presence of heterophile antibody interference in the Roche Elecsys assay is suspected when 1 or more of the following are ...
https://pediatric.testcatalog.org/show/IEHCG
Pesquisa | Prevenção e Controle de CâncerPesquisa | Prevenção e Controle de Câncer
... which include testing for specific IgM and IgG antibodies against viral capsid antigens, early antigens and EBV nuclear antigen ... However, up to 10% of patients with IM are heterophile negative. Heterophile-negative patients who have lymphocytosis or ... and a rapid heterophile antibody test. The heterophile antibody test has a sensitivity of 87% and specificity of 91% but can ... The heterophile antibody (also known as the Monospot) test is a useful screening tool for infectious mononucleosis (IM) ...
https://pesquisa.bvsalud.org/controlecancer/?lang=pt&q=mh:Mononucleose+Infecciosa
MonospotMonospot
Heterophile Antibody Test, Heterophil Agglutination Tube Test, Paul-Bunnell Test, Mononucleosis Heterophile Test, Heterophile ... Heterophile Antibodies (C0003248) Definition (CSP). antibody produced by the introduction of heterophile antigen into a species ... heterophile antibody, heterophile ab, heterophil antibody, heterophile antibodies, Heterophile Ab, Heterophile Antibody, ... HETEROPHILE ANTIBODIES SCREEN, Screening for heterophile antibodies, HETEROPHILE ANTIBODY SCREEN, Heterophile antibody screen. ...
https://fpnotebook.com/legacy/ID/Lab/Mnspt.htm
The comparative analysis of limfogramys indicators in adults with verified allegropathology on the background of replicative...The comparative analysis of limfogramy's indicators in adults with verified allegropathology on the background of replicative...
All patients were screened for the presence of worms (IEA for the presence of immunoglobulins A, M, G to antigens for Giardia, ... Votava, D. Bartosova, A. Krchnakova [et al.] «Diagnostic importance of heterophile antibodies and immunoglobulins IgA, IgE, IgM ... and low-avidity IgG against Epstein-Barr virus capsid antigen in children», Acta Virol. -1996.- V.40.- Р. 99-101. ...
https://euroasia-science.ru/medicinskie-nauki/the-comparative-analysis-of-limfogramy%E2%80%99s-indicators-in-adults-with-verified-allegropathology-on-the-background-of-replicative-virus-activity/
Browse by Publication Year : 2020-2029 : HistoryofMedicine.comBrowse by Publication Year : 2020-2029 : HistoryofMedicine.com
Epstein-Barr virus (EBV) antibodies-viral capsid antigen IgM and IgG, and EBV-associated nuclear antigen antibodies-were also ... A rapid point-of-care test for heterophile antibodies-the Monospot test-was negative, a full blood count with autodifferential ... A rapid antigen detection test for streptococcus was negative, and because of exudates on the patients tonsils and very ...
https://www.historyofmedicineandbiology.com/year/2020/
Epstein-Barr Virus (EBV) Infectious Mononucleosis (Mono): Background, Pathophysiology, EpidemiologyEpstein-Barr Virus (EBV) Infectious Mononucleosis (Mono): Background, Pathophysiology, Epidemiology
Marshall-Andon T, Heinz P. How to use … the Monospot and other heterophile antibody tests. Arch Dis Child Educ Pract Ed. 2017 ... II Viral capsid antigen. Table 2. EBV Serologic Responses in EBV-Associated Diseases ...
https://emedicine.staging.medscape.com/article/222040-overview
Laboratory diagnosis of epstein barr virus ebv )? how do you interpret epstein barr results, epstein-barr virus (ebv), also...Laboratory diagnosis of epstein barr virus ebv )? how do you interpret epstein barr results, epstein-barr virus (ebv), also...
... heterophile antibodies, and IgG antibodies to the EBV early antigen-diffuse (EA-D) and nuclear antigen (EBNA-1). The use of all ... viral capsid antigen (VCA) IgG, VCA IgM, and EBV nuclear antigen 1 IgG chemiluminescent immunoassays for detection of EBV ... EBV nuclear antigen (EBNA) IgG: Just like the VCA IgG these increase after the initial infection and then stay elevated for ... Here are the results of my EBV test: EBV Ab VCA, IgG, 260 EBV Ab VCA, IgM, less than 36 EBV Early Antigen Ab, IgG, 93.2 EBV ...
https://heldeccola.com/doi/abs/107lckcu212hg6w7.1111/cas
DeCS 2014 - December 16, 2014 version
Xenoantigens use Antigens, Heterophile Xenobiotics Xenodiagnosis Xenograft Antitumor Assays use Xenograft Model Antitumor ...
https://decs2020.bvsalud.org/I/DeCS2014_Alfab-X.htm
DeCS 2008 - versión 17 de Marzo de 2008
Xenoantibodies use Antibodies, Heterophile. Xenoantigens use Antigens, Heterophile. Xenobiotics. Xenodiagnosis. Xenograft use ...
https://decs2018.bvsalud.org/I/DeCS2008_Alfab-X_ing.htm
DeCS 2010 - February 12, 2010 version
Xenoantibodies use Antibodies, Heterophile Xenoantigens use Antigens, Heterophile Xenobiotics Xenodiagnosis Xenograft use ...
https://decs2014.bvsalud.org/I/DeCS2010_Alfab-X.htm
DeCS 2010 - February 12, 2010 version
Xenoantibodies use Antibodies, Heterophile Xenoantigens use Antigens, Heterophile Xenobiotics Xenodiagnosis Xenograft use ...
https://decs2013.bvsalud.org/I/DeCS2010_Alfab-X.htm
MonospotContaining heterophile antibodiesViralEBNAMononucleosisAssaysDetectionImmunoassaysProteinsNuclearPaul-BunnellSerumTestInfectionRecombinantReactSerologyInduceInfluenzaSpeciesProteinDiagnosticDescriptorNegativeAcuteTestsExposureLymphocytesOccurSpecificAssessmentPresencePositiveBloodTypePatient
Monospot7
  • Agglutination of horse RBCs on exposure to heterophile antibodies is the basis of the Monospot test. (medscape.com)
  • Rapid heterophile antibody testing (also referred to as Monospot) is often used as a first-line test in the diagnosis of EBV IM because it is inexpensive and has a fast turnaround. (arupconsult.com)
  • Heterophile antibody testing (also known as Monospot) is often used as a first-line test in the diagnosis of Epstein-Barr virus (EBV) infectious mononucleosis (IM). (arupconsult.com)
  • The heterophile antibody test (Monospot) is a standard diagnostic test for EBV IM in patients with symptoms of IM. (arupconsult.com)
  • A Monospot (rapid heterophile antibody) test is negative, but the test can be unreliable in young children. (contemporarypediatrics.com)
  • The heterophile antibody (also known as the Monospot) test is a useful screening tool for infectious mononucleosis (IM) resulting from primary Epstein-Barr virus (EBV) infection. (bvsalud.org)
  • Don't perform heterophile antibody (monospot) testing to diagnose acute Epstein-Barr virus (EBV) infection in children under 5 years of age. (labpulse.com)
Containing heterophile antibodies2
  • Manufacturers add blocking agents to their reagents, but occasional patient samples containing heterophile antibodies are incompletely blocked. (testcatalog.org)
  • Heterophile blocking tube (HBT) treatment is used for troubleshooting samples that yield results that are either non-linear or do not match the clinical picture of the patient and are suspected of containing heterophile antibodies. (testcatalog.org)
Viral5
  • IgM and IgG antibodies directed against viral capsid antigen has high sensitivity and specificity. (medscape.com)
  • Viral capsid antigen (VCA) - Anti-VCA IgM and anti-VCA IgG antibodies directed against EBV viral capsid antigen are present at the onset of infection. (medscape.com)
  • The presence or absence of EBV viral capsid ‎antigen (VCA) IgG, VCA IgM and EBV nuclear antigen (EBNA) IgG antibodies was recorded. (who.int)
  • Heterophile-negative patients who have lymphocytosis or atypical lymphocytes on peripheral blood smear should be further tested for EBV serologies, which include testing for specific IgM and IgG antibodies against viral capsid antigens, early antigens and EBV nuclear antigen proteins. (bvsalud.org)
  • The Epstein-Barr virus is a very common infection The diagnosis of infection due to herpesviruses can be performed by several laboratory techniques, including virus culture, demonstration of virus-specific cellular antigens, detection of virus-induced serum antibodies, detection of viral nucleic acids (using radiolabeled probes), or visualization of histologic changes in infected tissue How Do You Get Epstein-Barr Virus in the First Place? (heldeccola.com)
EBNA3
  • EBV Nuclear antigen (EBNA) - This is a protein expressed only when the virus begins to establish latency. (medscape.com)
  • EBV nuclear antigen (EBNA) IgG: Just like the VCA IgG these increase after the initial infection and then stay elevated for life. (heldeccola.com)
  • EBNA IgG (Epstein Barr Nuclear Antigen) - Indicates a history of infection in non-acute cases. (heldeccola.com)
Mononucleosis9
  • Since heterophile antibodies typically return quicker than EBV specific antibodies, it is the test of choice in most clinical settings to determine if an individual has infectious mononucleosis. (medscape.com)
  • One must consider that the heterophile antibody test results may be negative early in the course of EBV infectious mononucleosis. (medscape.com)
  • Patients who remain heterophile negative after 6 weeks with a mononucleosis illness should be considered as having heterophile-negative infectious mononucleosis. (medscape.com)
  • These may be considered in patients with suspected infectious mononucleosis who have a negative heterophile test. (medscape.com)
  • This test is often prescribed for adolescents exhibiting symptoms typical of mononucleosis, such as fever, fatigue, sore throat, swollen glands, etc Mono Test is intended for the qualitative detection of infectious mononucleosis heterophile antibodies in serum, plasma or whole blood as an aid in the diagnosis of infectious mononucleosis. (keresekvapnetbacio.com)
  • SUMMARY AND EXPLANATION OF TEST Our Mono test detects heterophile antibodies in the blood that are produced by the immune system as a response to fighting the Epstein-Barr Virus (EBV), the most common cause of mononucleosis. (keresekvapnetbacio.com)
  • For the qualitative detection of infectious mononucleosis heterophile antibodies in whole blood, serum or plasma. (carolinachemistries.com)
  • Herpesviruses: Human herpesvirus 6 - HHV-6: ELISA-VIDITEST and IF-VIDITEST anti-HHV-6 kits are intended for serological diagnosis of diseases associated with HHV-6 infection, such as exanthema subitum, acute respiratory illnesses, diarrhoea with fever and febrile seizures in infants, heterophile antibody-negative infectious mononucleosis in children, also interstitial pneumonia, encephalitis, meningitis, hepatitis and aplastic anemia in immunodeficient patients. (mobitec.de)
  • A small percentage of individuals will develop a negative heterophile-antibody infectious mononucleosis syndrome. (frenovobio.com)
Assays1
  • Among immunometric assays, human chorionic gonadotropin (hCG) assays have been found uniquely susceptible to heterophile antibody interference, resulting in occasional false-positive results. (testcatalog.org)
Detection3
  • However, previous studies indicate that sensitivity of p24 antigen detection for AHI might not exceed 80% ( 7 ). (cdc.gov)
  • [2] Otherwise, a rapid antigen detection test (RADT) or throat swab is recommended. (mdwiki.org)
  • Rapid detection of group A streptococcal antigen directly from throat swab specimens. (carolinachemistries.com)
Immunoassays2
  • Heterophile antibodies in plasma of certain individuals are known to cause interference with immunoassays. (cdc.gov)
  • The Centers for Disease Control and Prevention began addressing this problem by updating recommendations for the laboratory diagnosis of HIV in healthcare settings to include initial fourth generation HIV-1 p24 antigen-based immunoassays ( 6 ). (cdc.gov)
Proteins3
  • The QuantiFERON ® -TB Gold IT test is a test for Cell Mediated Immune (CMI) responses to petide antigens that simulate mycobacterial proteins. (cdc.gov)
  • The test is used to detect proteins in the blood called heterophile antibodies that are produced by the immune system in response to an Epstein-Barr virus (EBV) infection, the most common cause of mono Mono Test. (keresekvapnetbacio.com)
  • Study of the immune system analyses of blood that employ antigens (foreign proteins) and antibodie. (healthsfirst.in)
Nuclear1
  • Don't order antinuclear antibody (ANA) and extractable nuclear antigen (ENA) tests unless the patient is suspected to have a connective tissue disease. (labpulse.com)
Paul-Bunnell2
  • Sheep RBCs agglutinate in the presence of heterophile antibodies and are the basis for the Paul-Bunnell test. (medscape.com)
  • These antibodies are directed against a wide variety of interspecies-specific antigens, the best known of which are Forssman, Hanganutziu-Deicher (H-D), and Paul-Bunnell (P-B). Incidence of antibodies to these antigens--i.e., the phenomenon of heterophile antibody response--is useful in the serodiagnosis, pathogenesis, and prognosis of infection and latent infectious states as well as in cancer classification. (fpnotebook.com)
Serum1
  • The effect of heterophile antibodies in the QuantiFERON ® -TB Gold IT ELISA is minimized by the addition of normal mouse serum to the green diluent and the use of F(ab')2 monoclonal antibody fragments as the IFN-γ capture antibody coated the microplate wells. (cdc.gov)
Test16
  • 5. That a Hepatitis B surface antigen test not be performed if the person requesting the test indicates that the test is for a prenatal assessment. (ontario.ca)
  • however, the diagnosis should be confirmed with a heterophile antibody test or through EBV-specific antibodies. (medscape.com)
  • Once a reactive heterophile antibody is present, it is not necessary to test for specific antibodies to EBV. (medscape.com)
  • In patients with a compatible syndrome and negative heterophile antibodies, the test can be repeated if the patient is early in their clinical course. (medscape.com)
  • A test is considered positive for an IFN-γ response to the TB Antigen tube that is significantly above the Nil IFN-γ IU/mL value. (cdc.gov)
  • After incubation of the blood with antigens for 16 to 24 hours and in conjunction with a regularly scheduled shipment, specimens were sent to a contracted laboratory on frozen refrigerant packs or dry ice where the QFT-GIT test for tuberculosis infection was conducted. (cdc.gov)
  • In 1932, the association between the disease syndrome and a positive heterophile antibody test was recognized. (medscape.com)
  • EBV-specific serology can also be used in the place of heterophile antibody testing as an initial test. (arupconsult.com)
  • Although the CDC does not recommend the heterophile antibody test for general use, this test is often used as a first-line test because it is fast and inexpensive. (arupconsult.com)
  • In general, the heterophile antibody test should not be used in children younger than 5 years, most of whom do not produce the heterophile antibody. (arupconsult.com)
  • EBV-specific serology testing may also be used to diagnose EBV IM in place of a heterophile antibody test. (arupconsult.com)
  • The 4 tests are: EA IgG (Early Antigen) - this is the most important test and it's often missed. (heldeccola.com)
  • The main technical methods for COVID-19 testing include reverse transcription loop-mediated isothermal amplification (RT-LAMP), RT-PCR, serology test and antigen test. (ipscio.com)
  • The heterophile test can be used if this is suspected. (mdwiki.org)
  • An in vitro rapid qualitative test that detects influenza type A and type B antigens directly from nasal swab, nasopharyngeal swab and nasal aspirate/wash specimens. (carolinachemistries.com)
  • NOTE: The Coronavirus Aid, Relief, and Economic Security (CARES) Act require every CLIA certified COVID-19 testing site to report every diagnostic and screening test result (both positive and negative results) performed to detect SARS-CoV-2 or to diagnose a possible case of COVID-19 (e.g., molecular, antigen, antibody) to the appropriate state or local public health department, based on the individual's residence. (symbioticainc.com)
Infection4
  • Epstein-Barr virus (EBV) infection induces specific antibodies to EBV and various unrelated non-EBV heterophile antibodies. (medscape.com)
  • [ 32 ] Although anti-VCA IgM is highly suggestive of acute EBV infection, other herpes viruses (eg, cytomegalovirus) can induce IgM antibodies to cell lines that express EBV antigens. (medscape.com)
  • To determine the prevalence of TB infection, NHANES participants 6 years of age and older who consented to this component were skin tested with a tuberculin-purified protein derivative (PPD) product, tubersol, a commercially available antigen. (cdc.gov)
  • B-cell infection caused by EBV leads to the transformation of the B cells to immortal plasmacytoid cells, which secrete a wide variety of immunoglobulins (eg, heterophile antibodies). (medscape.com)
Recombinant1
  • Small synthetic peptides that mimic surface antigens of pathogens and are immunogenic, or vaccines manufactured with the aid of recombinant DNA techniques. (nih.gov)
React2
  • These heterophile antibodies react to antigens from animal RBCs. (medscape.com)
  • Antibodies that react with self-antigens ( AUTOANTIGENS ) of the organism that produced them. (nih.gov)
Serology1
  • If results are negative for EBV, serology tests for other virus-specific antigens (eg, cytomegalovirus , Toxoplasma gondii , human herpesvirus 6 , and HIV ) may be considered. (arupconsult.com)
Induce1
  • Antibodies, especially IGE, that bind to tissue of the same species so that ANTIGENS induce release of HISTAMINE and other vasoactive agents. (ouhsc.edu)
Influenza1
  • This study demonstrated that pregnant women were able to respond to an original myxovirus antigen, influenza A/New Jersey/8/76, in a manner equivalent to nonpregnant, age-matched controls. (elsevier.com)
Species3
  • They are cross-reacting antigens found in phylogenetically unrelated species. (bvsalud.org)
  • antibody produced by the introduction of heterophile antigen into a species which does not carry it. (fpnotebook.com)
  • Antibodies elicited in a different species from which the antigen originated. (fpnotebook.com)
Protein1
  • Prostate specific antigen (PSA) is a protein made by the prostate. (mjdrdypu.org)
Diagnostic2
  • Reactive heterophile antibodies in a patient with the typical symptoms are diagnostic of EBV and further testing for specific antibodies to EBV is not necessary. (medscape.com)
  • A diagnostic dilemma arises when the patient has clinical and laboratory features of IM, but is both heterophile negative and seronegative for IM, as illustrated in this case presentation. (bvsalud.org)
Descriptor1
  • Antigens, Bacterial" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (wakehealth.edu)
Negative2
  • 0.5 IU/mL) indicates an Indeterminate result when a blood sample also has a negative response to the TB antigens. (cdc.gov)
  • However, up to 10% of patients with IM are heterophile negative. (bvsalud.org)
Acute1
  • Early antigen (EA) - Anti-EA IgG appears during the acute phase and falls to undetectable levels after 3 to 6 months. (medscape.com)
Tests1
  • Heterophile antibodies against these tests so important role of dtc, some different antigenic site with suppressive functionality on? (redcedarassociates.com)
Exposure1
  • Some patients, due to exposure to animal antigens, have developed antibodies that interfere with immunoassay testing. (testcatalog.org)
Lymphocytes1
  • Individuals infected with M. tuberculosis complex organisms ( M. tuberculosis, M. bovis, M. africanum, M. microti, M. canetti ) usually have lymphocytes in their blood that recognize these and other mycobacterial antigens. (cdc.gov)
Occur2
  • Elevated levels of IFN-γ in the Nil sample may occur with the presence of heterophile antibodies, or to intrinsic IFN-γ secretion. (cdc.gov)
  • Analytical errors occur due to heterophile antibodies and macro-TSH. (mjdrdypu.org)
Specific2
  • The Nil samples adjust for background, heterophile antibody effects, or non-specific IFN-γ in blood samples. (cdc.gov)
  • Antibodies against specific EBV antigens and a number of autoantibodies also are produced. (medscape.com)
Assessment1
  • Finally, assessment of an analyte such as hCG with an alternative assay will often lead to apparent discrepant results in the presence of a heterophile antibody, as heterophile antibodies often interact differently with alternative assay antibodies. (testcatalog.org)
Presence1
  • Dilution of the specimen prior to assay performance often yields unexpected non-linear results in the presence of interfering substances such as heterophile antibodies. (testcatalog.org)
Positive1
  • The QuantiFERON ® -TB Gold IT system uses specialized blood collection tubes, which are used to collect whole blood via venipuncture, which include a Nil control tube, TB Antigen tube and a Mitogen tube (positive control). (cdc.gov)
Blood2
  • The tubes are shaken to mix antigen with the whole blood and incubated at 37° C + 1° C for 16 to 24 hours. (cdc.gov)
  • For each subject, 1 mL of blood was collected by venipuncture directly into each of three QFT GIT blood collection tubes in the order Nil (grey cap), TB Antigen (red cap), Mitogen (purple cap). (cdc.gov)
Type1
  • Urticarial vasculitis is a type III hypersensitivity reaction in which antigen-antibody complexes are deposited in the vascular lumina. (medscape.com)
Patient1
  • Un patient de 28 ans se présente au service des urgences pour prise en charge d'une douleur localisée au niveau de l'hypochondre gauche et du flanc gauche, apparue 48h avant son passage aux urgences. (bvsalud.org)
Epstein-Barr Virus (EBV) Infectious Mononucleosis (Mono) Workup: Approach Considerations, Laboratory Studies, Imaging Studies
Epstein-Barr Virus (EBV) Infectious Mononucleosis (Mono) Workup: Approach Considerations, Laboratory Studies, Imaging Studies (emedicine.medscape.com)
Infectious Mononucleosis (IM) in Emergency Medicine: Background, Pathophysiology, Epidemiology
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TC83 replicon vectored vaccine provides protection against Junin virus in guinea pigs - Fingerprint - UTMB Health Research...
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WHO EMRO | Trend and seroprevalence of Epstein-Barr virus in Bahrain: 2001-2015 | Volume 23, issue 12 | EMHJ volume 23, 2017
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Infectious Mononucleosis/Reactive Lymphocytes - A Laboratory Guide to Clinical Hematology
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Activation of natural killer cells and macrophages by porcine endothelial cells augments specific T-cell xenoresponse -...
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Upper Respiratory Tract Infection: Practice Essentials, Background, Pathophysiology
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Laboratory diagnosis of epstein barr virus ebv )? how do you interpret epstein barr results, epstein-barr virus (ebv), also...
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Royalty Report: Medical, Diagnostic, Device - Collection: 322230 - IPSCIO
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The antigenicity of serum proteins and their role in xenograft rejection. | Profiles RNS
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Antigens, Bacterial | Profiles RNS
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ELISA and Assay Reagents | ImmunoChemistry Technologies
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Mono test - a mononucleosis (mono) test is used to help diagnose mono,
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NLMID: 101584575X327 - Oswald T. Avery - Profiles in Science Search Results
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