A myelodysplastic-myeloproliferative disease characterized by monocytosis, increased monocytes in the bone marrow, variable degrees of dysplasia, but an absence of immature granulocytes in the blood.
A pediatric acute myeloid leukemia involving both myeloid and monocytoid precursors. At least 20% of non-erythroid cells are of monocytic origin.
A leukemia affecting young children characterized by SPLENOMEGALY, enlarged lymph nodes, rashes, and hemorrhages. Traditionally classed as a myeloproliferative disease, it is now considered a mixed myeloproliferative-mylelodysplastic disorder.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Substances that are recognized by the immune system and induce an immune reaction.
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
Substances elaborated by bacteria that have antigenic activity.
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
Substances elaborated by viruses that have antigenic activity.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Established cell cultures that have the potential to propagate indefinitely.
Form of leukemia characterized by an uncontrolled proliferation of the myeloid lineage and their precursors (MYELOID PROGENITOR CELLS) in the bone marrow and other sites.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Transforming proteins coded by myb oncogenes. Transformation of cells by v-myb in conjunction with v-ets is seen in the avian E26 leukemia virus.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Progenitor cells from which all blood cells derive.
Surface antigens expressed on myeloid cells of the granulocyte-monocyte-histiocyte series during differentiation. Analysis of their reactivity in normal and malignant myelomonocytic cells is useful in identifying and classifying human leukemias and lymphomas.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Any part or derivative of any protozoan that elicits immunity; malaria (Plasmodium) and trypanosome antigens are presently the most frequently encountered.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
An acute myeloid leukemia in which 80% or more of the leukemic cells are of monocytic lineage including monoblasts, promonocytes, and MONOCYTES.
Antigens determined by leukocyte loci found on chromosome 6, the major histocompatibility loci in humans. They are polypeptides or glycoproteins found on most nucleated cells and platelets, determine tissue types for transplantation, and are associated with certain diseases.
Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.
Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.
Antibodies produced by a single clone of cells.
Antigens expressed primarily on the membranes of living cells during sequential stages of maturation and differentiation. As immunologic markers they have high organ and tissue specificity and are useful as probes in studies of normal cell development as well as neoplastic transformation.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
The developmental history of specific differentiated cell types as traced back to the original STEM CELLS in the embryo.
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Clonal hematopoietic stem cell disorders characterized by dysplasia in one or more hematopoietic cell lineages. They predominantly affect patients over 60, are considered preleukemic conditions, and have high probability of transformation into ACUTE MYELOID LEUKEMIA.
Clonal expansion of myeloid blasts in bone marrow, blood, and other tissue. Myeloid leukemias develop from changes in cells that normally produce NEUTROPHILS; BASOPHILS; EOSINOPHILS; and MONOCYTES.
Substances of fungal origin that have antigenic activity.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism.
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
Any part or derivative of a helminth that elicits an immune reaction. The most commonly seen helminth antigens are those of the schistosomes.
An important regulator of GENE EXPRESSION during growth and development, and in NEOPLASMS. Tretinoin, also known as retinoic acid and derived from maternal VITAMIN A, is essential for normal GROWTH; and EMBRYONIC DEVELOPMENT. An excess of tretinoin can be teratogenic. It is used in the treatment of PSORIASIS; ACNE VULGARIS; and several other SKIN DISEASES. It has also been approved for use in promyelocytic leukemia (LEUKEMIA, PROMYELOCYTIC, ACUTE).
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
The major group of transplantation antigens in the mouse.
Leukocytes with abundant granules in the cytoplasm. They are divided into three groups according to the staining properties of the granules: neutrophilic, eosinophilic, and basophilic. Mature granulocytes are the NEUTROPHILS; EOSINOPHILS; and BASOPHILS.
The process in developing sex- or gender-specific tissue, organ, or function after SEX DETERMINATION PROCESSES have set the sex of the GONADS. Major areas of sex differentiation occur in the reproductive tract (GENITALIA) and the brain.
The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Conditions which cause proliferation of hemopoietically active tissue or of tissue which has embryonic hemopoietic potential. They all involve dysregulation of multipotent MYELOID PROGENITOR CELLS, most often caused by a mutation in the JAK2 PROTEIN TYROSINE KINASE.
A promyelocytic cell line derived from a patient with ACUTE PROMYELOCYTIC LEUKEMIA. HL-60 cells lack specific markers for LYMPHOID CELLS but express surface receptors for FC FRAGMENTS and COMPLEMENT SYSTEM PROTEINS. They also exhibit phagocytic activity and responsiveness to chemotactic stimuli. (From Hay et al., American Type Culture Collection, 7th ed, pp127-8)
Cells derived from the BLASTOCYST INNER CELL MASS which forms before implantation in the uterine wall. They retain the ability to divide, proliferate and provide progenitor cells that can differentiate into specialized cells.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
A glycoprotein that is secreted into the luminal surface of the epithelia in the gastrointestinal tract. It is found in the feces and pancreaticobiliary secretions and is used to monitor the response to colon cancer treatment.
Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
Leukemia induced experimentally in animals by exposure to leukemogenic agents, such as VIRUSES; RADIATION; or by TRANSPLANTATION of leukemic tissues.
Those proteins recognized by antibodies from serum of animals bearing tumors induced by viruses; these proteins are presumably coded for by the nucleic acids of the same viruses that caused the neoplastic transformation.
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Sites on an antigen that interact with specific antibodies.
A highly polar organic liquid, that is used widely as a chemical solvent. Because of its ability to penetrate biological membranes, it is used as a vehicle for topical application of pharmaceuticals. It is also used to protect tissue during CRYOPRESERVATION. Dimethyl sulfoxide shows a range of pharmacological activity including analgesia and anti-inflammation.
A subclass of HLA-D antigens that consist of alpha and beta chains. The inheritance of HLA-DR antigens differs from that of the HLA-DQ ANTIGENS and HLA-DP ANTIGENS.
Nuclear antigen with a role in DNA synthesis, DNA repair, and cell cycle progression. PCNA is required for the coordinated synthesis of both leading and lagging strands at the replication fork during DNA replication. PCNA expression correlates with the proliferation activity of several malignant and non-malignant cell types.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
A progressive, malignant disease of the blood-forming organs, characterized by distorted proliferation and development of leukocytes and their precursors in the blood and bone marrow. Leukemias were originally termed acute or chronic based on life expectancy but now are classified according to cellular maturity. Acute leukemias consist of predominately immature cells; chronic leukemias are composed of more mature cells. (From The Merck Manual, 2006)
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
IMMUNOGLOBULINS on the surface of B-LYMPHOCYTES. Their MESSENGER RNA contains an EXON with a membrane spanning sequence, producing immunoglobulins in the form of type I transmembrane proteins as opposed to secreted immunoglobulins (ANTIBODIES) which do not contain the membrane spanning segment.
Elements of limited time intervals, contributing to particular results or situations.
A group of antigens that includes both the major and minor histocompatibility antigens. The former are genetically determined by the major histocompatibility complex. They determine tissue type for transplantation and cause allograft rejections. The latter are systems of allelic alloantigens that can cause weak transplant rejection.
Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.
Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.
Large, transmembrane, non-covalently linked glycoproteins (alpha and beta). Both chains can be polymorphic although there is more structural variation in the beta chains. The class II antigens in humans are called HLA-D ANTIGENS and are coded by a gene on chromosome 6. In mice, two genes named IA and IE on chromosome 17 code for the H-2 antigens. The antigens are found on B-lymphocytes, macrophages, epidermal cells, and sperm and are thought to mediate the competence of and cellular cooperation in the immune response. The term IA antigens used to refer only to the proteins encoded by the IA genes in the mouse, but is now used as a generic term for any class II histocompatibility antigen.
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
A trisaccharide antigen expressed on glycolipids and many cell-surface glycoproteins. In the blood the antigen is found on the surface of NEUTROPHILS; EOSINOPHILS; and MONOCYTES. In addition, CD15 antigen is a stage-specific embryonic antigen.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Proteins prepared by recombinant DNA technology.
High-molecular weight glycoproteins uniquely expressed on the surface of LEUKOCYTES and their hemopoietic progenitors. They contain a cytoplasmic protein tyrosine phosphatase activity which plays a role in intracellular signaling from the CELL SURFACE RECEPTORS. The CD45 antigens occur as multiple isoforms that result from alternative mRNA splicing and differential usage of three exons.
The classes of BONE MARROW-derived blood cells in the monocytic series (MONOCYTES and their precursors) and granulocytic series (GRANULOCYTES and their precursors).
A human cell line established from a diffuse histiocytic lymphoma (HISTIOCYTIC LYMPHOMA, DIFFUSE) and displaying many monocytic characteristics. It serves as an in vitro model for MONOCYTE and MACROPHAGE differentiation.
The development and formation of various types of BLOOD CELLS. Hematopoiesis can take place in the BONE MARROW (medullary) or outside the bone marrow (HEMATOPOIESIS, EXTRAMEDULLARY).
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
Glycoproteins found on immature hematopoietic cells and endothelial cells. They are the only molecules to date whose expression within the blood system is restricted to a small number of progenitor cells in the bone marrow.
Stem cells derived from HEMATOPOIETIC STEM CELLS. Derived from these myeloid progenitor cells are the MEGAKARYOCYTES; ERYTHROID CELLS; MYELOID CELLS; and some DENDRITIC CELLS.
A glycoprotein that is a kallikrein-like serine proteinase and an esterase, produced by epithelial cells of both normal and malignant prostate tissue. It is an important marker for the diagnosis of prostate cancer.
Differentiation antigens found on thymocytes and on cytotoxic and suppressor T-lymphocytes. CD8 antigens are members of the immunoglobulin supergene family and are associative recognition elements in MHC (Major Histocompatibility Complex) Class I-restricted interactions.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
A phorbol ester found in CROTON OIL with very effective tumor promoting activity. It stimulates the synthesis of both DNA and RNA.
The lipopolysaccharide-protein somatic antigens, usually from gram-negative bacteria, important in the serological classification of enteric bacilli. The O-specific chains determine the specificity of the O antigens of a given serotype. O antigens are the immunodominant part of the lipopolysaccharide molecule in the intact bacterial cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.
Mapping of the KARYOTYPE of a cell.
Process of classifying cells of the immune system based on structural and functional differences. The process is commonly used to analyze and sort T-lymphocytes into subsets based on CD antigens by the technique of flow cytometry.
Proteins encoded by homeobox genes (GENES, HOMEOBOX) that exhibit structural similarity to certain prokaryotic and eukaryotic DNA-binding proteins. Homeodomain proteins are involved in the control of gene expression during morphogenesis and development (GENE EXPRESSION REGULATION, DEVELOPMENTAL).
The process of bone formation. Histogenesis of bone including ossification.
Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone.
Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
An acidic glycoprotein of MW 23 kDa with internal disulfide bonds. The protein is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. GM-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages.
Carbohydrate antigens expressed by malignant tissue. They are useful as tumor markers and are measured in the serum by means of a radioimmunoassay employing monoclonal antibodies.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
Membrane glycoproteins consisting of an alpha subunit and a BETA 2-MICROGLOBULIN beta subunit. In humans, highly polymorphic genes on CHROMOSOME 6 encode the alpha subunits of class I antigens and play an important role in determining the serological specificity of the surface antigen. Class I antigens are found on most nucleated cells and are generally detected by their reactivity with alloantisera. These antigens are recognized during GRAFT REJECTION and restrict cell-mediated lysis of virus-infected cells.
A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
Bone-marrow-derived, non-hematopoietic cells that support HEMATOPOETIC STEM CELLS. They have also been isolated from other organs and tissues such as UMBILICAL CORD BLOOD, umbilical vein subendothelium, and WHARTON JELLY. These cells are considered to be a source of multipotent stem cells because they include subpopulations of mesenchymal stem cells.
Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.
A myeloproliferative disorder characterized by neoplastic proliferation of erythroblastic and myeloblastic elements with atypical erythroblasts and myeloblasts in the peripheral blood.
A specific HLA-A surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-A*02 allele family.
Glycoproteins found on the membrane or surface of cells.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
An encapsulated lymphatic organ through which venous blood filters.
The differentiation of pre-adipocytes into mature ADIPOCYTES.
Methods for maintaining or growing CELLS in vitro.
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in leukemia.
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Sets of cell surface antigens located on BLOOD CELLS. They are usually membrane GLYCOPROTEINS or GLYCOLIPIDS that are antigenically distinguished by their carbohydrate moieties.
A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Embryonic (precursor) cells of the myogenic lineage that develop from the MESODERM. They undergo proliferation, migrate to their various sites, and then differentiate into the appropriate form of myocytes (MYOCYTES, SKELETAL; MYOCYTES, CARDIAC; MYOCYTES, SMOOTH MUSCLE).
Those hepatitis B antigens found on the surface of the Dane particle and on the 20 nm spherical and tubular particles. Several subspecificities of the surface antigen are known. These were formerly called the Australia antigen.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Clonal myeloid disorders that possess both dysplastic and proliferative features but are not properly classified as either MYELODYSPLASTIC SYNDROMES or MYELOPROLIFERATIVE DISORDERS.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.
Very large BONE MARROW CELLS which release mature BLOOD PLATELETS.
A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.
Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.
55-kDa antigens found on HELPER-INDUCER T-LYMPHOCYTES and on a variety of other immune cell types. CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in MAJOR HISTOCOMPATIBILITY COMPLEX class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as INTERLEUKIN-15 receptors and bind to the HIV receptors, binding directly to the HIV ENVELOPE PROTEIN GP120.
The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS.
Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.
Polymorphic class I human histocompatibility (HLA) surface antigens present on almost all nucleated cells. At least 20 antigens have been identified which are encoded by the A locus of multiple alleles on chromosome 6. They serve as targets for T-cell cytolytic responses and are involved with acceptance or rejection of tissue/organ grafts.
The sum of the weight of all the atoms in a molecule.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Normal cellular genes homologous to viral oncogenes. The products of proto-oncogenes are important regulators of biological processes and appear to be involved in the events that serve to maintain the ordered procession through the cell cycle. Proto-oncogenes have names of the form c-onc.
Glycoproteins expressed on cortical thymocytes and on some dendritic cells and B-cells. Their structure is similar to that of MHC Class I and their function has been postulated as similar also. CD1 antigens are highly specific markers for human LANGERHANS CELLS.
Developmental events leading to the formation of adult muscular system, which includes differentiation of the various types of muscle cell precursors, migration of myoblasts, activation of myogenesis and development of muscle anchorage.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
The cells in the granulocytic series that give rise to mature granulocytes (NEUTROPHILS; EOSINOPHILS; and BASOPHILS). These precursor cells include myeloblasts, promyelocytes, myelocytes and metamyelocytes.
A species of ALPHARETROVIRUS causing anemia in fowl.
Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. It occurs spontaneously or through interaction with viruses, oncogenes, radiation, or drugs/chemicals.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
Cells in the body that store FATS, usually in the form of TRIGLYCERIDES. WHITE ADIPOCYTES are the predominant type and found mostly in the abdominal cavity and subcutaneous tissue. BROWN ADIPOCYTES are thermogenic cells that can be found in newborns of some species and hibernating mammals.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
A cell line derived from cultured tumor cells.
The external, nonvascular layer of the skin. It is made up, from within outward, of five layers of EPITHELIUM: (1) basal layer (stratum basale epidermidis); (2) spinous layer (stratum spinosum epidermidis); (3) granular layer (stratum granulosum epidermidis); (4) clear layer (stratum lucidum epidermidis); and (5) horny layer (stratum corneum epidermidis).
Transport proteins that carry specific substances in the blood or across cell membranes.
An acute myeloid leukemia in which abnormal PROMYELOCYTES predominate. It is frequently associated with DISSEMINATED INTRAVASCULAR COAGULATION.
A protein found most abundantly in the nervous system. Defects or deficiencies in this protein are associated with NEUROFIBROMATOSIS 1, Watson syndrome, and LEOPARD syndrome. Mutations in the gene (GENE, NEUROFIBROMATOSIS 1) affect two known functions: regulation of ras-GTPase and tumor suppression.
The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.
A family of DNA-binding transcription factors that contain a basic HELIX-LOOP-HELIX MOTIF.
Human immune-response or Class II antigens found mainly, but not exclusively, on B-lymphocytes and produced from genes of the HLA-D locus. They are extremely polymorphic families of glycopeptides, each consisting of two chains, alpha and beta. This group of antigens includes the -DR, -DQ and -DP designations, of which HLA-DR is most studied; some of these glycoproteins are associated with certain diseases, possibly of immune etiology.
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
A receptor for MACROPHAGE COLONY-STIMULATING FACTOR encoded by the c-fms proto-oncogene (GENES, FMS). It contains an intrinsic protein-tyrosine kinase activity. When activated the receptor undergoes autophosphorylation, phosphorylation of down-stream signaling molecules and rapid down-regulation.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
Genes whose gain-of-function alterations lead to NEOPLASTIC CELL TRANSFORMATION. They include, for example, genes for activators or stimulators of CELL PROLIFERATION such as growth factors, growth factor receptors, protein kinases, signal transducers, nuclear phosphoproteins, and transcription factors. A prefix of "v-" before oncogene symbols indicates oncogenes captured and transmitted by RETROVIRUSES; the prefix "c-" before the gene symbol of an oncogene indicates it is the cellular homolog (PROTO-ONCOGENES) of a v-oncogene.
Retroviral proteins that have the ability to transform cells. They can induce sarcomas, leukemias, lymphomas, and mammary carcinomas. Not all retroviral proteins are oncogenic.
Molecules on the surface of B- and T-lymphocytes that recognize and combine with specific antigens.
Adherence of cells to surfaces or to other cells.
Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
The processes triggered by interactions of ANTIBODIES with their ANTIGENS.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
A multilineage cell growth factor secreted by LYMPHOCYTES; EPITHELIAL CELLS; and ASTROCYTES which stimulates clonal proliferation and differentiation of various types of blood and tissue cells.
A severe sometimes chronic anemia, usually macrocytic in type, that does not respond to ordinary antianemic therapy.
A basic enzyme that is present in saliva, tears, egg white, and many animal fluids. It functions as an antibacterial agent. The enzyme catalyzes the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. EC 3.2.1.17.
The physiologically active form of vitamin D. It is formed primarily in the kidney by enzymatic hydroxylation of 25-hydroxycholecalciferol (CALCIFEDIOL). Its production is stimulated by low blood calcium levels and parathyroid hormone. Calcitriol increases intestinal absorption of calcium and phosphorus, and in concert with parathyroid hormone increases bone resorption.
A cytologic technique for measuring the functional capacity of stem cells by assaying their activity.
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
Antigens of the virion of the HEPATITIS B VIRUS or the Dane particle, its surface (HEPATITIS B SURFACE ANTIGENS), core (HEPATITIS B CORE ANTIGENS), and other associated antigens, including the HEPATITIS B E ANTIGENS.
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
A mononuclear phagocyte colony-stimulating factor (M-CSF) synthesized by mesenchymal cells. The compound stimulates the survival, proliferation, and differentiation of hematopoietic cells of the monocyte-macrophage series. M-CSF is a disulfide-bonded glycoprotein dimer with a MW of 70 kDa. It binds to a specific high affinity receptor (RECEPTOR, MACROPHAGE COLONY-STIMULATING FACTOR).
An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.1.
Specialized forms of antibody-producing B-LYMPHOCYTES. They synthesize and secrete immunoglobulin. They are found only in lymphoid organs and at sites of immune responses and normally do not circulate in the blood or lymph. (Rosen et al., Dictionary of Immunology, 1989, p169 & Abbas et al., Cellular and Molecular Immunology, 2d ed, p20)
The rate dynamics in chemical or physical systems.
Glycolipid-anchored membrane glycoproteins expressed on cells of the myelomonocyte lineage including monocytes, macrophages, and some granulocytes. They function as receptors for the complex of lipopolysaccharide (LPS) and LPS-binding protein.
The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.
The GENETIC TRANSLATION products of the fusion between an ONCOGENE and another gene. The latter may be of viral or cellular origin.
A group of three different alpha chains (CD11a, CD11b, CD11c) that are associated with an invariant CD18 beta chain (ANTIGENS, CD18). The three resulting leukocyte-adhesion molecules (RECEPTORS, LEUKOCYTE ADHESION) are LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN-1; MACROPHAGE-1 ANTIGEN; and ANTIGEN, P150,95.

Expression and cellular localization of the CC chemokines PARC and ELC in human atherosclerotic plaques. (1/1497)

Local immune responses are thought to play an important role in the development of atherosclerosis. Histological studies have shown that human atherosclerotic lesions contain T lymphocytes throughout all stages of development, many of which are in an activated state. A number of novel CC chemokines have been described recently, which are potent chemoattractants for lymphocytes: PARC (pulmonary and activation-regulated chemokine), ELC (EBI1-ligand chemokine), LARC (liver and activation-regulated chemokine), and SLC (secondary lymphoid-tissue chemokine). Using reverse transcriptase-polymerase chain reaction and in situ hybridization, we have found gene expression for PARC and ELC but not for LARC or SLC in human atherosclerotic plaques. Immunohistochemical staining of serial plaque sections with specific cell markers revealed highly different expression patterns of PARC and ELC. PARC mRNA was restricted to CD68+ macrophages (n = 14 of 18), whereas ELC mRNA was widely expressed by macrophages and intimal smooth muscle cells (SMC) in nearly all of the lesions examined (n = 12 of 14). ELC mRNA was also found to be expressed in the medial SMC wall of highly calcified plaques (n = 4). Very low levels of ELC mRNA expression could also be detected in normal mammary arteries but no mRNA expression for PARC was detected in these vessels (n = 4). In vitro, ELC mRNA was found to be up-regulated in aortic SMC stimulated with tumor necrosis factor-a and interferon-gamma but not in SMC stimulated with serum. Both PARC and ELC mRNA were expressed by monocyte-derived macrophages but not monocytes. The expression patterns of PARC and ELC mRNA in human atherosclerotic lesions suggest a potential role for these two recently described CC chemokines in attracting T lymphocytes into atherosclerotic lesions.  (+info)

Phagocytosis stimulates alternative glycosylation of macrosialin (mouse CD68), a macrophage-specific endosomal protein. (2/1497)

Macrosialin (mouse CD68), a macrophage-specific member of the lysosomal-associated membrane protein family, displays N-linked glycosylation and a heavily sialylated, mucin-like domain. We show that phagocytosis of zymosan by inflammatory peritoneal macrophages potently alters glycan processing of macrosialin in vitro. The phagocytic glycoform is not induced by other forms of endocytosis and depends on particle internalization. Zymosan uptake does not influence macrosialin protein synthesis, but increases the specific incorporation of D-[2-3H]mannose, D-[6-3H]galactose, N-acetyl-D-[1-3H]glucosamine and L-[5,6-3H]fucose by 2-15-fold. The phagocytic glycoform displays increased binding of agglutinins from peanut, Amaranthus caudatus and Galanthus nivalis, whereas binding of the sialic-acid-specific Maakia amurensis agglutinin is slightly reduced. Digestion by N-Glycanase abolishes the incorporation of [3H]mannose label and Galanthus nivalis agglutinin binding activity, but preserves the incorporation of galactose and N-acetylglucosamine and specific lectin binding. We also show that phagocytosis increases the complexity and length of O-linked chains. The data presented highlight the importance of differential glycosylation in the biology of macrosialin, phagosomes and macrophages in general.  (+info)

Immunohistochemical analysis of arterial wall cellular infiltration in Buerger's disease (endarteritis obliterans). (3/1497)

PURPOSE: The diagnosis of Buerger's disease has depended on clinical symptoms and angiographic findings, whereas pathologic findings are considered to be of secondary importance. Arteries from patients with Buerger's tissue were analyzed histologically, including immunophenotyping of the infiltrating cells, to elucidate the nature of Buerger's disease as a vasculitis. METHODS: Thirty-three specimens from nine patients, in whom Buerger's disease was diagnosed on the basis of our clinical and angiographic criteria between 1980 and 1995 at Nagoya University Hospital, were studied. Immunohistochemical studies were performed on paraffin-embedded tissue with a labeled streptoavidin-biotin method. RESULTS: The general architecture of vessel walls was well preserved regardless of the stage of disease, and cell infiltration was observed mainly in the thrombus and the intima. Among infiltrating cells, CD3(+) T cells greatly outnumbered CD20(+) B cells. CD68(+) macrophages or S-100(+) dendritic cells were detected, especially in the intima during acute and subacute stages. All cases except one showed infiltration by the human leukocyte antigen-D region (HLA-DR) antigen-bearing macrophages and dendritic cells in the intima. Immunoglobulins G, A, and M (IgG, IgA, IgM) and complement factors 3d and 4c (C3d, C4c) were deposited along the internal elastic lamina. CONCLUSION: Buerger's disease is strictly an endarteritis that is introduced by T-cell mediated cellular immunity and by B-cell mediated humoral immunity associated with activation of macrophages or dendritic cells in the intima.  (+info)

Increased poly(ADP-ribosyl)ation of nuclear proteins in Alzheimer's disease. (4/1497)

Experimental studies indicate that overactivation of the DNA repair protein poly(ADP-ribose) polymerase (PARP) in response to oxidative damage to DNA can cause cell death due to depletion of NAD+. Oxidative damage to DNA and other macromolecules has been reported to be increased in the brains of patients with Alzheimer's disease. In the present study we sought evidence of PARP activation in Alzheimer's disease by immunostaining sections of frontal and temporal lobe from autopsy material of 20 patients and 10 controls, both for PARP itself and for its end-product, poly(ADP-ribose). All of the brains had previously been subjected to detailed neuropathological examination to confirm the diagnosis of Alzheimer's disease or, in the controls, to exclude Alzheimer's disease-type pathology. Double immunolabelling for poly(ADP-ribose) and microtubule-associated protein 2 (MAP2), glial fibrillary-acidic protein (GFAP), CD68, A beta-protein or tau was used to assess the identity of the cells with poly(ADP-ribose) accumulation and their relationship to plaques and neurofibrillary tangles. Both PARP- and poly(ADP-ribose)-immunolabelled cells were detected in a much higher proportion of Alzheimer's disease (20 out of 20) brains than of control brains (5 out of 10) (P = 0.0018). Double-immunolabelling for poly(ADP-ribose) and markers of neuronal, astrocytic and microglial differentiation (MAP2, GFAP and CD68, respectively) showed many of the cells containing poly(ADP-ribose) to be neurons. Most of these were small pyramidal neurons in cortical laminae 3 and 5. A few of the cells containing poly(ADP-ribose) were astrocytes. No poly(ADP-ribose) accumulation was detected in microglia. Double-immunolabelling for poly(ADP-ribose) and tau or A beta-protein indicated that the cells with accumulation of poly(ADP-ribose) did not contain tangles and relatively few occurred within plaques. Our findings indicate that there is enhanced PARP activity in Alzheimer's disease and suggest that pharmacological interventions aimed at inhibiting PARP may have a role in slowing the progression of the disease.  (+info)

Identification of the block in targeted retroviral-mediated gene transfer. (5/1497)

A chimeric retroviral vector (33E67) containing a CD33-specific single-chain antibody was generated in an attempt to target cells displaying the CD33 surface antigen. The chimeric envelope protein was translated, processed, and incorporated into viral particles as efficiently as wild-type envelope protein. The viral particles carrying the 33E67 envelope protein could bind efficiently to the CD33 receptor on target cells and were internalized, but no gene transfer occurred. A unique experimental approach was used to examine the basis for this postbinding block. Our data indicate that the chimeric envelope protein itself cannot participate in the fusion process, the most reasonable explanation being that this chimeric protein cannot undergo the appropriate conformational change that is thought to be triggered by receptor binding, a suggested prerequisite to subsequent fusion and core entry. These results indicate that the block to gene transfer in this system, and probably in most of the current chimeric retroviral vectors to date, is the inability of the chimeric envelope protein to undergo this obligatory conformational change.  (+info)

CD40 expression on graft infiltrates and parenchymal CD154 (CD40L) induction in human chronic renal allograft rejection. (6/1497)

BACKGROUND: CD40-CD154 (CD40L) costimulatory signaling plays a pivotal role in the effector mechanisms of transplant graft rejection. In animal models, CD40-CD154 blockade induces long-term graft acceptance concurrent with an absence of chronic rejection (CR) lesions. Given the critical importance of CD40-CD154 interactions in the development of chronic transplant allograft rejection, the relevance of in situ CD40 and CD154 expression was assessed in human chronic renal allograft rejection. METHODS: The expression of CD40, CD154, CD68, and T-cell receptor (TCR)alpha/beta was analyzed by immunohistochemistry. Serial cryostat sections of snap-frozen core renal allograft biopsies were obtained from 30 renal transplant patients. Biopsy specimens received diagnoses of CR (N = 23) according to the Banff classification and were compared with controls (N = 7) consisting of stable allografts and normal kidney tissue. RESULTS: Striking CD40 staining of graft cellular infiltrates (P = 0.016) was observed in renal allografts with CR compared with controls. The CD40+ cellular infiltrates in CR were predominantly TCR alpha/beta + T cells and some CD68+ macrophages. These findings were contrasted by the low-level CD40 expression detected in glomeruli and tubules of CR and controls. However, glomerular induction of CD154 was observed in CR allografts (P = 0.028) as compared with controls. CD154 immunoreactivity was demonstrated on glomerular endothelial, epithelial, and mesangial cells. Moderate CD154 expression was detected on tubular epithelial cells, and only weak CD154 immunoreactivity was observed on the infiltrates in isolated CR cases. CONCLUSION: In human chronic renal allograft rejection, CD40 is expressed on graft-infiltrating cells of the T cell and macrophage compartments. CD154 expression is induced on glomerular and tubular epithelial cells during CR, demonstrating another novel source of CD154 expression. The data substantiate the potential contributory role of an interaction between CD40+ graft-destructive effector T cells and macrophages with CD154+ renal allograft parenchymal cells in the development of chronic renal allograft rejection.  (+info)

The myeloid-specific sialic acid-binding receptor, CD33, associates with the protein-tyrosine phosphatases, SHP-1 and SHP-2. (7/1497)

The myeloid restricted membrane glycoprotein, CD33, is a member of the recently characterized "sialic acid-binding immunoglobulin-related lectin" family. Although CD33 can mediate sialic acid-dependent cell interactions as a recombinant protein, its function in myeloid cells has yet to be determined. Since CD33 contains two potential immunoreceptor tyrosine-based inhibition motifs in its cytoplasmic tail, we investigated whether it might act as a signaling receptor in myeloid cells. Tyrosine phosphorylation of CD33 in myeloid cell lines was stimulated by cell surface cross-linking or by pervanadate, and inhibited by PP2, a specific inhibitor of Src family tyrosine kinases. Phosphorylated CD33 recruited both the protein-tyrosine phosphatases, SHP-1 and SHP-2. CD33 was dephosphorylated in vitro by the co-immunoprecipitated tyrosine phosphatases, suggesting that it might also be an in vivo substrate. The first CD33 phosphotyrosine motif is dominant in CD33-SHP-1/SHP-2 interactions, since mutating tyrosine 340 in a CD33-cytoplasmic tail fusion protein significantly reduced binding to SHP-1 and SHP-2 in THP-1 lysates, while mutation of tyrosine 358 had no effect. Furthermore, the NH2-terminal Src homology 2 domain of SHP-1 and SHP-2, believed to be essential for phosphatase activation, selectively bound a CD33 phosphopeptide containing tyrosine 340 but not one containing tyrosine 358. Finally, mutation of tyrosine 340 increased red blood cell binding by CD33 expressed in COS cells. Hence, CD33 signaling through selective recruitment of SHP-1/SHP-2 may modulate its ligand(s) binding activity.  (+info)

Expression of the activation antigen CD97 and its ligand CD55 in rheumatoid synovial tissue. (8/1497)

OBJECTIVE: Fibroblast-like synoviocytes (FLS) express decay-accelerating factor (CD55) at high levels. Recently, it was found that CD55 is a specific cellular ligand for the 7-span transmembrane receptor CD97. The objective of this study was to define the expression of this receptor-ligand pair in synovial tissue (ST) to provide more insight into the interaction between FLS and surrounding cells. METHODS: Antibodies against CD97 and CD55 were used for immunohistologic analysis of synovial biopsy specimens from 16 patients with rheumatoid arthritis (RA) and 15 patients with osteoarthritis (OA). In addition, an enzyme-linked immunosorbent assay system was used to determine the expression of soluble CD97 (sCD97) in synovial fluid (SF) from 30 patients with RA, 13 with OA, and 10 with reactive arthritis (ReA). RESULTS: In both RA and OA ST sections, strong expression of CD55 was confirmed on FLS in the intimal lining layer, where it was also found that all macrophages expressed CD97. The percentage of macrophages that expressed CD97 was lower in the synovial sublining (P = 0.005). The mean levels of sCD97 in SF were significantly higher in RA patients than in patients with OA or ReA (P < 0.0001). CONCLUSION: These results suggest that FLS are able to interact with macrophages via the CD97/CD55 receptor-ligand system. In this respect, the CD97/CD55 pair may account for the specific architecture of the intimal lining layer and may be of primary importance in maintaining and amplifying synovial inflammation. The specific increase in sCD97 levels in RA SF might be related to the presence of activated proteolytic systems or to the increase in synovial mass, rather than a consequence of local receptor-ligand interaction.  (+info)

The Anti-Siglec-H antibody reacts with the 34 kDa mouse sialic acid-binding immunoglobulin-like lectin (Siglec) H. Siglec-H is specifically expressed on mouse plasmacytoid dendritic cells1 - a subset of CD11c+ dendritic cells detected at low frequency in all lymphoid tissues, peripheral blood, and some non-lymphoid tissues. Binding of antibodies to Siglec-H inhibits type I interferon production, which can be induced in plasmacytoid dendritic cells by DNA and RNA viruses.2,3 - USA
The Anti-Siglec-H antibody reacts with the 34 kDa mouse sialic acid-binding immunoglobulin-like lectin (Siglec) H. Siglec-H is specifically expressed on mouse plasmacytoid dendritic cells1 - a subset of CD11c+ dendritic cells detected at low frequency in all lymphoid tissues, peripheral blood, and some non-lymphoid tissues. Binding of antibodies to Siglec-H inhibits type I interferon production, which can be induced in plasmacytoid dendritic cells by DNA and RNA viruses.2,3 | USA
One hundred and ninety-four adult patients admitted to a Department of Internal Medicine at a tertiary hospital with suspected community acquired infections. Daily blood sampling for sCD163 analysis was performed for up to 5 days. Laboratory analyses were performed with a sandwich ELISA using polyclonal rabbit anti-CD163 as the catching antibody and monoclonal anti-CD163 as the secondary antibody. The patients were classified according to SIRS criteria. The patients were divided in the following groups: patients with no proven infection (n = 67, control group), patients with possible infection (n = 21, not included in analysis), patients with proven infection without SIRS (n = 25), patients with sepsis (n = 52), patients with severe sepsis (n = 29). Only one patient had septic shock. Twelve patients had bacteraemia. Demographic data, comorbidity, microbiological aetiology, biochemical parameters, focus of infection, severity score and mortality on day 28 were recorded. ...
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In this report, we show that blockade of CSF1/CSF1R signaling in pancreatic tumors depletes CD206Hi TAMs and reprograms remaining macrophages to support antitumor immunity. The blockade alone modestly enhances antitumor IFN responses, promotes CTL infiltration, and slows tumor progression. However, the therapeutic effect is limited by the induction of T-cell checkpoint molecules, including PDL1 on tumor cells and CTLA4 on T cells. Addition of the CSF1/CSF1R blockade markedly improved the efficacy of αPD1 and αCTLA4 checkpoint immunotherapy and led to the regression of even well-established PDAC tumors. These data suggest that CSF1/CSF1R signaling may be an effective therapeutic target to reprogram the immunosuppressive microenvironment of human PDAC tumors and enhance the efficacy of immunotherapy.. Recent data from several groups suggest that inhibition of CSF1R signaling alters the immunologic responses of tumor-infiltrating macrophages in several cancer types (10-12, 31-33). Mok and ...
CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia ...
CD33 (gp62 or siglec-3) is a glycosylated transmembrane protein that is a member of the sialic acid-binding immunoglobulin-like lectin (siglec) family. The genomic locus of this protein has been mapped to chromosome 19q13.1-3.5. The function of CD33 is not known, but it may have a role in cell-to-cell adhesion. In maturing granulocytic cells, there is progressive down-regulation of CD33 from the blast stage to mature neutrophils. However, in monocytes and macrophages/histiocytes, strong expression of CD33 is maintained throughout maturation ...
Rabbit polyclonal antibody raised against synthetic peptide of MNDA. A synthetic peptide corresponding to MNDA. (PAB25135) - Products - Abnova
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F4/80 -----Original Message----- From: [email protected] [mailto:[email protected]]On Behalf Of GT Hebert Sent: 11 July 2005 17:30 To: [email protected] Subject: [Histonet] Macrophage marker mouse tissue Hello, I am in need of a good macrophage marker on FFPE tissue. I will be using it on mouse tissue... mainly the heart (but other tissues may be included). So far I have looked into a number of antibodies (CD68, Mac-2) but can never be certain which one to choose. Thank you in advance for your help. Sincerely, Gustave Gustave Hebert, HT (ASCP) Scientist II Wyeth Research Cardiovascular and Metabolic Diseases Cambridge MA --------------------------------- Sell on Yahoo! Auctions - No fees. Bid on great items. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing ...
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Cynomolgus Siglec-15, His Tag (SG5-C52H6) is expressed from human 293 cells (HEK293). It contains AA Phe 20 - Thr 263 (Accession # A0A2K5UY47-1).
First-generation immune checkpoint inhibitors, including anti-CTLA-4 and anti-programmed death 1 (anti-PD-1) antibodies, have led to major clinical progress, yet resistance frequently leads to treatment failure. Thus, new targets acting on T cells are needed. CD33-related sialic acid-binding immunoglobulin-like lectins (Siglecs) are pattern-recognition immune receptors binding to a range of sialoglycan ligands, which appear to function as self-associated molecular patterns (SAMPs) that suppress autoimmune responses. Siglecs are expressed at very low levels on normal T cells, and these receptors were not until recently considered as interesting targets on T cells for cancer immunotherapy. Here, we show an upregulation of Siglecs, including Siglec-9, on tumor-infiltrating T cells from non-small cell lung cancer (NSCLC), colorectal, and ovarian cancer patients. Siglec-9-expressing T cells coexpressed several inhibitory receptors, including PD-1. Targeting of the sialoglycan-SAMP/Siglec pa
TY - JOUR. T1 - Characterization of expression of glycan ligands for Siglec-F in normal mouse lungs. AU - Guo, Jin P.. AU - Brummet, Mary E.. AU - Myers, Allen C.. AU - Na, Ho Jeong. AU - Rowland, Elizabeth. AU - Schnaar, Ronald L.. AU - Zheng, Tao. AU - Zhu, Zhou. AU - Bochner, Bruce S.. PY - 2011/2/1. Y1 - 2011/2/1. N2 - Sialic acid-binding immunoglobulin-like lectin (Siglec)-F, an inhibitory receptor on mouse eosinophils, preferentially recognizes the glycan ligand 69-sulfated sialyl Lewis X, but little is known about the requirements for its lung expression. RT-PCR and immunohistochemistry were used to detect and localize the sulfotransferase keratin sulfate galactose 6-O sulfotransferase (KSGal6ST, alsoknown as carbohydrate sulfotransferase 1; gene name, Chst1) that is putatively required for 6′-sulfated Sialyl Lewis X synthesis. RT-PCR detected the greatest constitutive expression of Chst1 in lung, liver, andspleen tissue.Immunohistochemistry localized the expression of KSGal6ST in lung ...
First-generation immune checkpoint inhibitors, including anti-CTLA-4 and anti-programmed death 1 (anti-PD-1) antibodies, have led to major clinical progress, yet resistance frequently leads to treatment failure. Thus, new targets acting on T cells are needed. CD33-related sialic acid-binding immunoglobulin-like lectins (Siglecs) are pattern-recognition immune receptors binding to a range of sialoglycan ligands, which appear to function as self-associated molecular patterns (SAMPs) that suppress autoimmune responses. Siglecs are expressed at very low levels on normal T cells, and these receptors were not until recently considered as interesting targets on T cells for cancer immunotherapy. Here, we show an upregulation of Siglecs, including Siglec-9, on tumor-infiltrating T cells from non-small cell lung cancer (NSCLC), colorectal, and ovarian cancer patients. Siglec-9-expressing T cells coexpressed several inhibitory receptors, including PD-1. Targeting of the sialoglycan-SAMP/Siglec pathway in ...
Sepsis is the most frequent cause of death in hospitalized patients, and severe sepsis is a leading contributory factor to acute respiratory distress syndrome (ARDS). At present, there is no effective treatment for these conditions, and care is primarily supportive. Murine sialic acid-binding immunoglobulin-like lectin-E (Siglec-E) and its human orthologs Siglec-7 and Siglec-9 are immunomodulatory receptors found predominantly on hematopoietic cells. These receptors are important negative regulators of acute inflammatory responses and are potential targets for the treatment of sepsis and ARDS. We describe a Siglec-targeting platform consisting of poly(lactic-co-glycolic acid) nanoparticles decorated with a natural Siglec ligand, di(α2→8) N-acetylneuraminic acid (α2,8 NANA-NP). This nanoparticle induced enhanced oligomerization of the murine Siglec-E receptor on the surface of macrophages, unlike the free α2,8 NANA ligand. Furthermore, treatment of murine macrophages with these nanoparticles ...
Tumor-infiltrating immune cells are a hallmark of most solid tumors, and the presence of varied immune populations significantly affects clinical outcomes for patients with cancer (1, 2). Historically, tumor-infiltrating immune cells have been viewed as restraining tumor progression (3), but in recent years, it has become more widely appreciated that chronic immune responses play critical roles in promoting tumor progression, metastasis, and resistance to cytotoxic therapies (1). Therefore, understanding the molecular mechanisms by which malignant cells derail antitumor immune responses to favor disease progression is critical to identify potential therapeutic targets. Recently, we reported that selective depletion of tumor-infiltrating macrophages (TAM) by neutralizing colony-stimulating factor-1 (CSF1) or inhibiting CSF1 receptor (CSF1R) activity improves the efficacy of chemotherapy in mammary tumors, in part by instigating antitumor responses by CD8+ T cells (4). Similarly, deficiency in the ...
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SIGLEC H, PerCP-eFluor™ 710, clone: eBio440c, eBioscience™ 100μg; PerCP-eFluor™ 710 SIGLEC H, PerCP-eFluor™ 710, clone: eBio440c,...
Abstract: Siglecs are a family of sialic acid-recognizing immunoglobulin-like lectins that exhibit multiple human-specific and human-universal differences, including changes in binding specificity (Siglec-5, -7, -9, -11, -12 and 14); changes in expression pattern (Siglec-1, -5, -6, and -11); gene conversion (SIGLEC11); gene deletion (SIGLEC13) and pseudogenization (SIGLEC17). Human-unique pseudogenes of SIGLEC12, SIGLEC14 and SIGLEC16 are also polymorphic within human populations, suggesting ongoing selection on this family of genes. The apparently higher concentration of SIGLEC changes in the human lineage may have been selected by interactions with pathogens binding Siglecs, and/or as compensatory responses to the loss of the sialic acid N-glycolylneuraminic acid (Neu5Gc) in humans. Human-specific Siglec changes of particular interest include expression of Siglec-11 in brain microglia, expression of Siglec-6 on placental trophoblast, suppression of Siglec-5 expression on adaptive immune cells, ...
Prospective, multicenter, uncontrolled cohort study to analyze the efficacy of a risk adapted treatment strategy, including gemtuzumab ozogamicin (GO) d
Siglec-15 serves as a paradigm for several siglecs, including Siglec-14[1], Siglec-16[2] and Siglec-H[3][4], that contain a basic amino acid within the transmembrane domain[5]. This leads to association of these siglecs with a transmembrane adaptor protein containing an immunoreceptor tyrosine based activation motif (ITAM). Siglec-15 is unusual compared to other siglecs that share this paradigm in two respects. Firstly it can associate with two ITAM containing adaptors, DAP12 and DAP10, whereas Siglec-14, Siglec-16, and Siglec-H show a restricted association with DAP12. Siglec-15 is also unusual in having four cysteine residues in the V-set domain predicted to result in an inter-sheet disulfide that is absent from all other known siglecs. These potentially activating siglecs are expressed on myeloid cells and dendritic cells and may be involved in innate responses to pathogen challenge. ...
Linköping University, Faculty of Health Sciences. Linköping University, Department of Clinical and Experimental Medicine, Pediatrics . Östergötlands Läns Landsting, Centre of Paediatrics and Gynecology and Obstetrics, Department of Paediatrics in Linköping. ...
A delicate balance between inducers and inhibitors of apoptosis exists in any given cell (37). In cancer cells, including melanoma, this equilibrium is often skewed in favor of survival, with IAPs being predominant (38, 39). Committing melanoma cells to apoptosis, which would be an ideal outcome for most therapies, therefore requires additional stimuli. Inhibition of IAPs for one can restore the balance between survival and death signals, thereby facilitating programmed cell death in melanoma.. Chronic inflammation in the tumor microenvironment of melanoma lesions often leads to elevated levels of TNF-α, at least in part, provided by the tumor-infiltrating immune cells such as macrophages (23-25). Levels of tumor-infiltrating macrophages have been shown to be associated with aggressive disease (40). All melanoma cell lines tested were resistant to treatment with exogenous TNF-α as a single agent. This was in line with previous clinical experiences, where minimal antitumor effect and ...
Siglec-H, 0.5 mg. Siglec-H, or sialic acid binding immunoglobulin-like lectin H, is a CD33 related protein expressed specifically by plasmacytoid dendritic cells or pDCs (1, 2).
This phase II trial studies the how well fractionated gemtuzumab ozogamicin works in treating measurable residual disease in participants with acute myeloid leukemia. Immunotherapy with monoclonal antibodies, such as gemtuzumab ozogamicin, may help the bodys immune system attack the cancer, and may interfere with the ability of tumor cells to grow and spread. ...
Activation of monocyte-derived cells occurs at most sites of inflammation and serves as a first line of defense in the immune system by removing foreign objects...
Background: EBUS-TBNA is performed to harvest cytologic and core-biopsy specimens in patients with mediastinal lesions. Rapid cytologic examinations of the sample are expected to increase the diagnostic rates. We studied the value of USB microscope as Rapid On-site Microscopic Evaluation (ROME) of core-biopsy specimens.. Methods: We performed ROME in 20 core-biopsy specimens sampled by EBUS-TBNA. The specimens were divided into five groups according to the microscopic findings. The group I was classified when whitish tumor islets were not found. The group II has one or several tiny suspicious tumor islets. The group III has less than 5 visible tumor islets, the group IV 5 - 10, and the group V more than 10 tumor islets. The ROME was compared with the final pathologic examination. Results: Among 20 specimens, 3 (15%) were in group I, 2 (10%) in group II, 5 (25%) in group III, 7 (35%) in group IV, and 3 (15%) in group V, respectively. When compared with the pathologic examination, clinical ...
Siglec G Mouse anti-Mouse, PerCP-eFluor 710, Clone: SH2.1, eBioscience™ 25μg; PerCP-eFluor 710 Siglec G Mouse anti-Mouse, PerCP-eFluor 710, Clone: SH2.1,...
Use Bio-Rads PrimePCR assays, controls, templates for your target gene. Every primer pair is optimized, experimentally validated, and performance guaranteed.
Polyclonal antibody for Siglec 2/CD22 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. Siglec 2/CD22 information: Molecular Weight: 95348 MW; Subcellular Localization: Cell membrane; Single-pass type I membran
Varje måndag, klockan 14:15 till 16:00, ges seminarier om aktuell forskning på Vi2 i rum 4307, hus 4, Polacksbacken, Uppsala. Ibland ges även extra seminarier vid andra tillfällen än den ovan nämnda tiden. Seminarierna är öppna för alla som är intresserade av ämnet.. ...
PRIMARY OBJECTIVES:. I. To study safety and efficacy single agent Gemtuzumab Ozogamicin (Mylotarg®) as induction therapy for patients with Acute Myeloid Leukemia (AML) who have relapsed after standard treatments or who are not candidates for standard consolidation treatment after Daunorubicin and cytosine arabinoside.. SECONDARY OBJECTIVES:. I. To correlate morbidity and mortality with the use of gemtuzumab (gemtuzumab ozogamicin) to specific subtypes of leukemia.. II. To correlate gemtuzumab response to degree of cluster of differentiation (CD) 33 positivity.. III. To correlate FMS-Related Tyrosine Kinase 3 (FLT 3)/nucleophosmin (NPM) status and CD 33 positivity to gemtuzumab response.. IV. To document incidence of sinusoidal obstruction syndrome with the use of gemtuzumab.. OUTLINE:. Patients receive gemtuzumab ozogamicin intravenously (IV) over 2 hours on days 1 and 15. Treatment continues for 28 days in the absence of disease progression or unacceptable toxicity.. After completion of study ...
Hepatotoxicity, including life-threatening and sometimes fatal hepatic VOD events, have been reported in patients receiving MYLOTARG as a single agent or as part of a combination chemotherapy regimen [see Adverse Reactions (6)].. In ALFA-0701, VOD events were reported in 6/131 (5%) adult patients during or following treatment with MYLOTARG, or following later hematopoietic stem cell transplantation (HSCT). The median time from the MYLOTARG dose to onset of VOD was 9 days (range: 2-298 days), with 5 events occurring within 28 days of any dose of MYLOTARG and 1 event occurring greater than 28 days after the last dose of MYLOTARG. Three of the 6 VOD events were fatal. VOD was also reported in 2 patients in the control arm of ALFA-0701 after receiving MYLOTARG as a therapy for relapsed AML.. In MyloFrance-1 (MYLOTARG 3 mg/m2 on Days 1, 4 and 7), VOD events were reported in none of the 57 patients during or following treatment, or following HSCT after completion of MYLOTARG treatment.. In AAML0531, ...
Sialic-acid-binding immunoglobulin-like lectins (Siglecs) are members of the Ig superfamily that bind sialic acids in different linkages in a wide variety of glycoconjugates. These membrane receptors are expressed in a highly specific manner, predominantly within the haematopoietic system. The CD33- …
Tumor-associated macrophages (TAMs) are the multifarious group of cells that originate mainly from the peritumoral tissue or bone marrow and can be divided into two main types: M1 and M2. Among them are the infiltrating M1 tumor-associated macrophages present in the early stages of tumorigenesis, which can secrete proinflammatory cytokines and in turn inhibit tumor growth. On the contrary, M2 tumor-associated macrophages are predominant in the late stage of tumor formation. Type II cytokines, which are secreted by them, can promote anti-inflammatory reaction and thus promote tumor growth. However, it remains unclear when M1 tumor-associated macrophages are transformed to M2 tumor-associated macrophages, but tumor hypoxia is currently thought to be associated with such a shift. M2 tumor-associated macrophages secrete many proteases such as cathepsin, cytokines, and an epidermal growth factor. The presence of M2 TAMs make the tumor prone to growth and angiogenesis, which in turn damages other ...
Objectives: To evaluate the impact of myeloid antigen expression on complete remission (CR), event-free survival (EFS), and overall survival (OS) in patients with T-cell acute lymphoblastic leukemia (T-ALL) treated with intensive chemotherapy. Methods: We retrospectively reviewed consecutive patients diagnosed with T-ALL and treated in Sultan Qaboos University Hospital and Royal Hospital in Oman between 2004 and 2010. The diagnosis of T-ALL was established using French-American-British classification or World Health Organization criteria. Patients were considered having myeloid antigen expression if they expressed CD13, CD33, or both (My+ and My-). Results: Of the 39 patients, 38 were included in the study (25 patients with My- and median age of 18.4 years, 13 patients with My+ and median age of 22.0 years). Median follow-up was 12 months. Thirty-two out of the total cohort were eligible for response-rate assessment. Twenty-nine patients (90.6%) achieved CR with one or two courses of ...
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SAN DIEGO -- Oncologists are re-evaluating gemtuzumab ozogamicin (Mylotarg) in acute myeloid leukemia, with new results presented here suggesting its 2010 withdrawal from the market may have been prem
ALS researchers from around the world gathered in Berlin, Germany, Dec. 8-10, to report on and discuss the latest research advances in this disease. Daily updates from the symposium are available online at the Web sites of the Motor Neurone Disease Association (MNDA Symposium reports) and ALS Therapy Development Institute (ALS TDI Symposium reports). ...
Siglecs (sialic acid-binding lg-like lectins) are mainly expressed in the immune system. Sn (sialoadhesin) (siglec-1), CD22 (siglec-2) and siglec-15 are well ...
Polyclonal antibody for SIGLEC 4A/MAG detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. SIGLEC 4A/MAG information: Molecular Weight: 69069 MW; Subcellular Localization: Membrane; Single-pass type I membrane protei
Abcams Siglec 9 ELISA Kit suitable for Cell culture supernatant, Serum, Plasma in human. Reliably quantify 130 pg/ml of Siglec 9.
TY - CONF. T1 - The Role of Gemtuzumab Ozogamicin in Elderly AML Patients in Complete Remission. AU - Siragusa, Sergio. PY - 2007. Y1 - 2007. N2 - The majority of patients (pts) with acute myeloid leukemia (AML) are diagnosed in their 6th and 7th decade of life. AML in elderly pts is associated with poor response to conventional chemotherapy and limited long-term survival, reflecting a higher incidence multidrug resistance mechanisms, a low bone marrow reserve which may prevent/delay the recovery of hematopoiesis after treatment, and the occurrence of co-morbidities. Gemtuzumab ozogamicin (GO) is an immunoconjugate with a humanized anti-CD33 that after internalization, releases a cytotoxic drug, calicheamicin; ≥80% of AML pts have myeloid blast cells that express the CD33 surface antigen. GO as a single agent has low antileukemic activity (Sievers et al, J Clin Oncol 2001;19:3244-54). However, GO is being used at lower doses in combined chemotherapy regimens as induction or postremission ...
In this study we immunophenotypically differentiate subpopulations of brain macrophages into perivascular macrophages and parenchymal microglia and demonstrate that perivascular macrophages are the major cell productively infected by SIV in the CNS of macaques. Preferential infection of perivascular macrophages in the CNS may account for several important observations concerning infection of the CNS, viral dynamics in the CNS, and the role of the CNS as a viral sanctuary or reservoir.. Although it has not been directly demonstrated, it is generally assumed that lentiviruses enter the CNS by the traffic of infected monocyte/macrophages (64). Our data showing that perivascular macrophages are the major cell type, infected in the brain, support this hypothesis. Studies in chimeric rodents and humans receiving bone marrow indicate that perivascular macrophages are continuously replaced from the circulation (15)(16)(17)(43). The immunophenotype described for perivascular macrophages, ...
R01 AI072265, NIH/NIAID - Bochner (PI). Studies in this grant will explore whether Siglec-8, a molecule selectively expressed by eosinophils and mast cells, can be targeted for both diagnostic and therapeutic purposes in allergic, gastrointestinal and malignant diseases.. Siglecs (sialic acid-binding, immunoglobulin-like lectins) are cell surface proteins found predominantly on leukocytes. Siglec-8 was discovered by us about a decade ago and is selectively expressed on eosinophils and mast cells. Its closest functional paralog in the mouse is Siglec-F, which is also selectively expressed by eosinophils but unfortunately not on mast cells. Both Siglec-8 and Siglec-F preferentially and uniquely recognize the glycan 6-sulfo-sialyl Lewis X (6-sulfo-sLeX) and its non-fucosylated form. Engagement of Siglec-8/-F with antibodies (Abs) and/or artificial ligands causes eosinophil death. Administration of Siglec-F Abs in mouse models of chronic allergic asthma and eosinophilia normalizes eosinophilic ...
Gustave We use F4/80 as a macrophage marker in mice. We have also tried MAC-2. Both work fine on FFPE tissue. Most of our clients ask for the F4/80 marker. We purchase ours from serotec. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 [email protected] www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of GT Hebert Sent: Monday, July 11, 2005 9:30 AM To: [email protected] Subject: [Histonet] Macrophage marker mouse tissue Hello, I am in need of a good macrophage marker on FFPE tissue. I will be using it on mouse tissue... mainly the heart (but other tissues may be included). So far I have looked into a number of antibodies (CD68, Mac-2) but can never be certain which ...
1O9Z: The Fimbrial Adhesin F17-G of Enterotoxigenic Escherichia Coli Has an Immunoglobulin-Like Lectin Domain that Binds N-Acetylglucosamine
Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the contacts provided below. For general information, Learn About Clinical Studies. ...
This information from Lexicomp® explains what you need to know about this medication, including what its used for, how to take it, its side effects, and when to call your healthcare provider.
Metcalfe, H J and Best, A and Kanellos, T and La Radione, R M and Werling, D (2010) Flagellin expression enhances Salmonella accumulation in TLR5-positive macrophages. Developmental and Comparative Immunology, 34 (8). pp. 797-804. ...
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Sialic acid binding immunoglobulin-type lectin (Siglec) family are inhibitory receptors with diverse roles in the immune system. Siglec family contains 14 members in human and 9 in murine. Differentially expressed on various white blood cells. Here in this review we are focusing on CD22, also known as Sialic Acid-Binding Ig-Like Lectin 2 (Siglec-2). CD22 gene is located on 19q13.12 and is encoding a 140 kD type I transmembrane glycoprotein on the surface of B cells and is part of the immunoglobulin (Ig) superfamily and has been found only on B cells. CD22 has been shown to play a major role in establishing a baseline level of B-cell inhibition, and thus is a critical determinant of homeostasis in humoral immunity ...
The US Food and Drug Administration recently approved gemtuzumab ozogamicin (Mylotarg) for the treatment of patients 60 years of age and older who are in first relapse with CD33-positive acute myeloid leukemia (AML) and are not considered 1
In this study, we tried to reveal the molecular and cellular mechanisms of activation of anti-tumor immunity by CD169-positive macrophages located in lymph node sinus. We generated CD169-Cre-YFP mice, in which CD169-positive macrophages were specifically labeled with YFP, and examined the character of these cells using the mice. We also identified CD169 macrophages-producing molecules that may be able to activate anti-tumor immunity. We established an experimental model to induce cell death of tumor cells in tumor-bearing mice, found the drastic changes in tumor-associated macrophages after tumor cell death. We also reveal the roles of CD169 macrophages in the immune regulation in non-immune tissues. ...
Expression of the mannose receptor (MRC1/CD206) identifies macrophage subtypes, such as alternatively activated macrophages (AAMs) and M2-polarized tumor-associated macrophages (TAMs), which are endowed with tissue-remodeling, proangiogenic, and protumoral activity. However, the significance of MRC1 …
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T lymphocytes regulate the growth and differentiation of T cells and certain B cells through the release of secreted protein ... IL3 is produced by T lymphocytes and T-cell lymphomas only after stimulation with antigens, mitogens, or chemical activators ... However, IL3 is constitutively expressed in the myelomonocytic leukaemia cell line WEHI-3B. It is thought that the genetic ... It plays an essential role in the final differentiation of B cells into immunoglobulin-secreting cells, as well as inducing ...
... antigens, cd56 MeSH D23.101.100.894.157 - antigens, cd57 MeSH D23.101.100.900 - antigens, differentiation, myelomonocytic MeSH ... antigens, cd57 MeSH D23.050.301.264.900 - antigens, differentiation, myelomonocytic MeSH D23.050.301.264.900.045 - antigens, ... antigens, differentiation, t-lymphocyte MeSH D23.101.100.894.080 - antigens, cd1 MeSH D23.101.100.894.090 - antigens, cd2 MeSH ... antigens, cd29 MeSH D23.050.301.264.894 - antigens, differentiation, t-lymphocyte MeSH D23.050.301.264.894.080 - antigens, cd1 ...
2011 Expression of NOV and BNIP3 gene in mouse myelomonocytic leukemia and its significance 2011 Relationship between WT1- ... differentiation and cell cycle distribution of mouse thymocytes after acute radiation 2011 "CM-DiI labeled mesenchymal stem ... tumor antigen 1-specific T lymphocyte generation soon after nonmyeloablative allergenic stem-cell transplantation in acute and ...
These are phagocytosis, antigen presentation, and cytokine production. Phagocytosis is the process of uptake of microbes and ... and differentiation into macrophages or dendritic cells to effect an immune response. In an adult human, half of the monocytes ... "Disappearance of slan-positive non-classical monocytes for diagnosis of chronic myelomonocytic leukemia with an associated ... "Differentiation of monocytes". Journal of Cell Biology. 50 (2): 498-515. doi:10.1083/jcb.50.2.498. PMC 2108281. PMID 4107019. ...
Hirohashi N, Nakao M, Kubo K, Yamada A, Shichijo S, Hara A, Sagawa K, Itoh K (Dec 1993). "A novel antigen (H47 Ag) on human ... Cluster of differentiation ENSG00000276452, ENSG00000277134, ENSG00000274669, ENSG00000277807 GRCh38: Ensembl release 89: ... "A common inhibitory receptor for major histocompatibility complex class I molecules on human lymphoid and myelomonocytic cells ... The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a ...
"Entrez Gene: SPECC1 sperm antigen with calponin homology and coiled-coil domains 1". Olsen JV, Blagoev B, Gnad F, et al. (2006 ... 2005). "Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation". ... "HCMOGT-1 is a novel fusion partner to PDGFRB in juvenile myelomonocytic leukemia with t(5;17)(q33;p11.2)". Cancer Res. 64 (8): ...
Cluster of differentiation ENSG00000274587, ENSG00000277816, ENSG00000204577 GRCh38: Ensembl release 89: ENSG00000275019, ... 1997). "A novel inhibitory receptor (ILT3) expressed on monocytes, macrophages, and dendritic cells involved in antigen ... "A common inhibitory receptor for major histocompatibility complex class I molecules on human lymphoid and myelomonocytic cells ... The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a ...
IL-3 is capable of stimulating differentiation of immature myelomonocytic cells causing changes to the macrophage and ... IL-3 is produced by T cells only after stimulation with antigens or other specific impulses. However, it was observed that IL-3 ... IL-3 receptors can be found on a variety of cell types including many immature myelomonocytic cells in the hemopoietic system ... Activated T cells can either induce their own proliferation and differentiation (autocrine signaling), or that of other T cells ...
1999). "Tetrameric complexes of human histocompatibility leukocyte antigen (HLA)-G bind to peripheral blood myelomonocytic ... Cluster of differentiation ENSG00000275463, ENSG00000131042, ENSG00000276146, ENSG00000277751 GRCh38: Ensembl release 89: ... 1998). "Human myelomonocytic cells express an inhibitory receptor for classical and nonclassical MHC class I molecules". J. ... The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a ...
Morphine causes inflammation by binding to the protein lymphocyte antigen 96, which, in turn, causes the protein to bind to ... TLR4 has also been designated as CD284 (cluster of differentiation 284). The molecular weight of TLR4 is approximately 95 kDa. ... This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. It cooperates ... TLR4 has been shown to interact with: Lymphocyte antigen 96, Myd88, and TOLLIP. Nickel, Intracellular trafficking of TLR4 is ...
Leukocyte immunoglobulin-like receptor subfamily A member 3 (LILR-A3) also known as CD85 antigen-like family member E (CD85e), ... with a preference for free heavy chains of HLA-C alleles Cluster of differentiation "Human PubMed Reference:". National Center ... "A common inhibitory receptor for major histocompatibility complex class I molecules on human lymphoid and myelomonocytic cells ... and can bind human leukocyte antigen (HLA) class I. Therefore, if secreted, the LILRA3 might impair interactions of membrane- ...
... antigen - antigen-presenting cell - antigen-presenting cell vaccine - antiglobulin test - antihormone therapy - antimetabolite ... chronic myelomonocytic leukemia - chronic phase - chronic phase chronic myelogenous leukemia - CHS 828 - CI-1033 - CI-958 - CI- ... cell differentiation - cell motility - cell proliferation - cell respiration - cell adhesion - cellular adoptive immunotherapy ... human leukocyte antigen - human lymphocyte antigen - human papillomavirus - human T-cell leukemia virus type 1 - Hürthle cell ...
For example, the presence of Sialyl-Lewisx antigen (cluster of differentiation 15s (CD15s)), which is one of SeV cell entry ... A novel myelomonocytic cell surface antigen and its distribution on leukemic cells". International Journal of Cancer. 33 (5): ... November 2015). "CD Nomenclature 2015: Human Leukocyte Differentiation Antigen Workshops as a Driving Force in Immunology". ... and sialyl Tn antigens in colorectal cancer patients: multivariate analysis of predictive factors for serum antigen levels". ...
2005). "Ligands for natural killer cell-activating receptors are expressed upon the maturation of normal myelomonocytic cells ... NCR3 has also been designated as CD337 (cluster of differentiation 337) and as NKp30. ENSG00000236979, ENSG00000206430, ... Tissue Antigens. 58 (4): 255-8. doi:10.1034/j.1399-0039.2001.580406.x. PMID 11782277. "Entrez Gene: NCR3 natural cytotoxicity ...
The FLT3 gene codes for the cluster of differentiation antigen 135 (i.e. CD135) protein or FLT3 protein. This protein is a ... or chronic myelomonocytic leukemia with involvement of tonsils. Some of these patients may present with little or no ... It mediates at least in part the cell proliferating signaling stimulated by PDGF receptors as well as by antigen receptors on T ... PDGFRA, through its tyrosine kinase activity, contributes to the growth, differentiation, and proliferation of cells. ...
Five regions of the 3' UTR that appear to bind proteins were found, one of which is HuR, a tumor antigen. HuR binds to AU-rich ... In addition to neurofibromatosis type I, mutations in NF1 can also lead to juvenile myelomonocytic leukemias (JMML), ... cell differentiation or migration. Neurofibromin is also known to interact with CASK through syndecan, a protein which is ... Haeussler J, Haeusler J, Striebel AM, Assum G, Vogel W, Furneaux H, Krone W (January 2000). "Tumor antigen HuR binds ...
... also plays a role in juvenile myelomonocytic leukemia (JMML). Studies have shown the protein's involvement in the disease ... and B-cell antigen receptors". Blood. 93 (6): 1809-16. doi:10.1182/blood.V93.6.1809.406k35_1809_1816. PMID 10068651. "Entrez ... "A novel role for Gab2 in bFGF-mediated cell survival during retinoic acid-induced neuronal differentiation". The Journal of ... "Regulation of the Erk2-Elk1 signaling pathway and megakaryocytic differentiation of Bcr-Abl(+) K562 leukemic cells by Gab2". ...
PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, ... "Human myelomonocytic cells express an inhibitory receptor for classical and nonclassical MHC class I molecules". J. Immunol. ... "SHP-1 requires inhibitory co-receptors to down-modulate B cell antigen receptor-mediated phosphorylation of cellular substrates ...
CAR-T treatment has significant side effects, and loss of the antigen targeted by the CAR-T cells is a common mechanism for ... Treatment for juvenile myelomonocytic leukemia can include splenectomy, chemotherapy, and bone marrow transplantation. Before ... differentiation or division. These mutations may occur spontaneously or as a result of exposure to radiation or carcinogenic ... One such approach used genetically modified T cells, known as chimeric antigen receptor T cells (CAR-T cells), to attack cancer ...
... atypical chronic myelomonocytic leukemia, juvenile myelomonocytic leukemia, myelodysplastic syndrome, acute myelogenous ... Similarly, differentiation of adipose from pericytes and mesenchymal cells is suppressed. Misregulation of the PDGFRβ's kinase ... CD140B+Antigen at the US National Library of Medicine Medical Subject Headings (MeSH) Biology portal. ... Mice harboring a single activated allele of PDGFRB show a number of postnatal phenotypes including reduced differentiation of ...
Antigen processing Apoptosis Biogenesis of organelles Cell cycle and division DNA transcription and repair Differentiation and ... "Germline CBL mutations cause developmental abnormalities and predispose to juvenile myelomonocytic leukemia". Nature Genetics. ... Proliferating cell nuclear antigen (PCNA) is a protein involved in DNA synthesis. Under normal physiological conditions PCNA is ... Ishikura S, Weissman AM, Bonifacino JS (July 2010). "Serine residues in the cytosolic tail of the T-cell antigen receptor alpha ...
"Requirement of tyrosine-phosphorylated Vav for morphological differentiation of all-trans-retinoic acid-treated HL-60 cells". ... of the c-cbl protooncogene is the 120-kDa tyrosine-phosphorylated protein in Jurkat cells activated via the T cell antigen ... article describing CBL function at PDBe OMIM enteries on NOONAN SYNDROME-LIKE DISORDER WITH OR WITHOUT JUVENILE MYELOMONOCYTIC ...
One such antigen was MAGE-A1. The coexistence of a progressing melanoma with melanoma-specific T cells implicitly does not ... Myelomonocytic cell products that cause apoptosis include FasL, TNF-α, and TNF-related apoptosis-inducing ligand (TRAIL). ... February 2015). "Invasive breast cancer reprograms early myeloid differentiation in the bone marrow to generate ... Preclinical mice studies implicate CAFs, TAMs and myelomonocytic cells (including several myeloid-derived suppressor cells ( ...
Myelomonocytic" by people in Harvard Catalyst Profiles by year, and whether "Antigens, Differentiation, Myelomonocytic" was a ... "Antigens, Differentiation, Myelomonocytic" is a descriptor in the National Library of Medicines controlled vocabulary ... Below are the most recent publications written about "Antigens, Differentiation, Myelomonocytic" by people in Profiles. ... Antigens, Differentiation, Myelomonocytic*Antigens, Differentiation, Myelomonocytic. *Myelomonocytic Differentiation Antigens. ...
Antigens, CD* * Antigens, Differentiation, Myelomonocytic / genetics * Carrier Proteins / genetics * Cyclic AMP Response ... M130 antigen, and peptidyl-prolyl isomerase. Those that were significantly decreased (15% to 35%) were SAS transmembrane 4 ...
Antigens, CD / metabolism * Antigens, Differentiation, Myelomonocytic / metabolism * Half-Life * Humans * Interferon-gamma / ...
Antigens, Differentiation, Myelomonocytic. Surface antigens expressed on myeloid cells of the granulocyte-monocyte-histiocyte ... series during differentiation. Analysis of their reactivity in normal and malignant myelomonocytic cells is useful in ... Differentiation of monocytes to macrophages increased galectin-9, and nonclassic monocytes from hepatitis C virus patients had ... Differentiation of monocytes to macrophages increased galectin-9. Nonclassic monocytes from hepatitis C virus patients express ...
0 (Anti-Citrullinated Protein Antibodies); 0 (Antigens, CD); 0 (Antigens, Differentiation, Myelomonocytic); 0 (CD68 antigen, ... 0 (Antigens, CD); 0 (Antigens, Differentiation, Myelomonocytic); 0 (Lectins); 0 (Receptor for Advanced Glycation End Products ... 0 (Antigens, CD); 0 (B7-H1 Antigen); 0 (Biomarkers, Tumor); 0 (CD223 antigen); 0 (CD274 protein, human); 0 (CD8 Antigens); 0 ( ... 0 (AC133 Antigen); 0 (Antigens, CD); 0 (Biomarkers, Tumor); 0 (CDH1 protein, human); 0 (CDH2 protein, human); 0 (Cadherins); 0 ...
Antigens, CD / metabolism. Antigens, Differentiation, Myelomonocytic / metabolism. Benzamides / pharmacology*. Blotting, ... 0/Antigens, Differentiation, Myelomonocytic; 0/Benzamides; 0/CD68 antigen, human; 0/Enzyme Inhibitors; 0/Madh2 protein, rat; 0/ ... 0/4-(4-(3-(pyridin-2-yl)-1H-pyrazol-4-yl)pyridin-2-yl)-N-(tetrahydro-2H-pyran-4-yl)benzamide; 0/Actins; 0/Antigens, CD; ...
0/Antigens, CD; 0/Antigens, Differentiation, Myelomonocytic; 0/Antirheumatic Agents; 0/Biological Markers; 0/CD68 antigen, ... Antigens, CD / metabolism. Antigens, Differentiation, Myelomonocytic / metabolism. Antirheumatic Agents / therapeutic use*. ...
Antigens, CD14 [D23.050.301.264.035.114]. *Antigens, Differentiation, Myelomonocytic [D23.050.301.264.900]. *Antigens, CD14 [ ... "Antigens, CD14" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... This graph shows the total number of publications written about "Antigens, CD14" by people in Harvard Catalyst Profiles by year ... Below are the most recent publications written about "Antigens, CD14" by people in Profiles. ...
Six monoclonal antibodies (MAb) which react with myelomonocytic cells representing various stages of differentiation, and which ... Biochemistry and expression of myelomonocytic antigens.. S M Goyert, E M Ferrero, S V Seremetis, R J Winchester, J Silver, A C ... Biochemistry and expression of myelomonocytic antigens.. S M Goyert, E M Ferrero, S V Seremetis, R J Winchester, J Silver, A C ... Biochemistry and expression of myelomonocytic antigens.. S M Goyert, E M Ferrero, S V Seremetis, R J Winchester, J Silver and A ...
A monoclonal antibody (MMA) that identifies a differentiation antigen on human myelomonocytic cells. Clin. Immunol. ... Monoclonal antibodies to human myelomonocyte differentiation antigens in the diagnosis of acute myeloid leukemia. Med. Oncol. ... The cutaneous lymphocyte antigen is a skin lymphocyte homing receptor for the vascular lectin endothelial cell-leukocyte ... A distinct type of sialyl Lewis X antigen defined by a novel monoclonal antibody is selectively expressed on helper memory T ...
Antigens, CD/analysis. *Antigens, Differentiation, Myelomonocytic/analysis. *Cell Differentiation. *Cell Proliferation. *Cells ... Conclusion: IL-1beta and IL-3 stimulated endothelium induces proliferation and differentiation of myeloid precursors, while IL- ... Conclusion: IL-1beta and IL-3 stimulated endothelium induces proliferation and differentiation of myeloid precursors, while IL- ... Hematopoietic progenitor cells and interleukin-stimulated endothelium: expansion and differentiation of myeloid precursors. ...
Antigens, CD/metabolism. *Antigens, CD86/metabolism. *Antigens, Differentiation, Myelomonocytic/metabolism. *Calcium-Binding ...
... of the CD11b subunit is restricted to cells of the myelomonocytic lineage and depends upon the stage of differentiation with ... The CD11b (or macrophage-1 antigen; MAC-1) subunit of the leukocyte integrin family forms a noncovalently associated ... Identification of the promoter of the myelomonocytic leukocyte integrin CD11b. D D Hickstein, D M Baker, K A Gollahon, A L Back ... Identification of the promoter of the myelomonocytic leukocyte integrin CD11b. D D Hickstein, D M Baker, K A Gollahon, A L Back ...
Complex pattern of the myelo-monocytic differentiation antigens MRP8 and MRP14 during chronic airway inflammation. ...
AML cases appear to have unique immature characteristics including low expression of myelomonocytic differentiation antigens ( ... The typical expression pattern of myelomonocytic differentiation antigens and cytokine receptors in CD4+ AML was ... The typical expression pattern of myelomonocytic differentiation antigens and cytokine receptors in CD4+ AML was CD34lowCD33 ... AML cases appear to have unique immature characteristics including low expression of myelomonocytic differentiation antigens ( ...
Leukemia stem cells also possess surface antigen profiles and signal transduction activation profiles which may allow ... Leukemia stem cells also possess surface antigen profiles and signal transduction activation profiles which may allow ... Antigens Used in Targeting AML LSCs. CD33. CD33 is a sialylated protein expressed on normal cells with myelomonocytic ... H90 targets LSCs in human AML through niche disruption and with evidence of LSC differentiation and loss of LSC self-renewal ...
... of bone marrow samples from 50 patients with MDS showed aberrant expression of differentiation antigens in the myelomonocytic ... In 49 patients with MDS defined by current morphology criteria, aberrant expression of differentiation antigens was ... aberrant expression of differentiation antigens was demonstrated in one or more subpopulations. Multiple aberrancies in ... Highly consistent differentiation patterns were seen in control samples in concordance with previous reports.5,6,8⇓⇓⇓⇓-13,15⇓- ...
113 consecutive patients with hematologic malignancies who received non-T-cell-depleted BMT from human leukocyte antigen (HLA)- ... cells under the influence of cytokines that act during early myelomonocytic differentiation.[25-32] Phenotypic and functional ... Antigen-primed dendritic cells have been used with promising results in adoptive vaccination against tumors.[34,35] Bone marrow ... Heath WR, Kurts C, Miller JF, et al: Cross-tolerance: A pathway for inducing tolerance to peripheral tissue antigens. J Exp Med ...
... that identifies a differentiation antigen on human myelomonocytic cells, Clin. Immunol. Immunopathol. 23:172.PubMedCrossRef ... Monoclonal antibodies that detect differentiation surface antigens on human myelomonocytic cells, Blood 59:382.PubMedGoogle ... Terminal differentiation surface antigens of myelomonocytic cells are expressed in human promyelocytic leukemia cells (HL60) ... Expression of normal monocyte-macrophage differentiation antigens on HL-60 promyelocytes undergoing differentiation induced by ...
1981) Terminal differentiation surface antigens of myelomonocytic cells are expressed in human promyelocytic leukemia cells ( ... 1992) Antigen uptake and presentation by dendritic leukocytes. Semin Immunol 4:227-236, pmid:1391797.. ... Cells were treated with PMA to induce differentiation to monocytic cells or with DMSO to induce granulocytic cells. MDC ... During this process of maturation, they lose their ability to process and present soluble antigen and become extemely potent ...
Induction of Myelomonocytic Differentiation.. A number of myeloid leukemia cell lines have been shown to differentiate in ... 32 ). Surface expression of myelomonocytic antigen CD11b was determined by indirect immunofluorescence staining and flow ... Myelomonocytic differentiation was assessed by monitoring the reduction of NBT, nonspecific α-naphthyl acetate esterase, ... 5)⇓ . Expression of CD11b, another typical myelomonocytic differentiation marker, was greatly enhanced by actinomycin D, but ...
Y. Chen, E. M. Akirav, W. Chen et al., "RAGE ligation affects T cell activation and controls T cell differentiation," Journal ... The RAGE-Mac-1 interaction has been shown to mediate the adhesion of neutrophils and myelomonocytic cells to immobilized RAGE ... RAGE expressed on T cells plays an essential role in the antigen-activated proliferative response. In a study on RAGE deficient ... or RAGE-transfected cells [2, 47]. RAGE on T cells is associated with the differentiation of this cell type, as RAGE activation ...
The CD14 antigen is found on cells of myelomonocytic lineage and is stronly expressed on monocytes and macrophages. CD14 is ... The antibodies were prepared against human leukocyte differentiation antigens. Each product is supplied in phosphate buffered ...
Antigens, Differentiation, Myelomonocytic. *Lipopolysaccharide Receptors. LinkOut - more resources. Full Text Sources. * ... Summary of workshop findings for porcine myelomonocytic markers.. Thacker E1, Summerfield A, McCullough K, Ezquerra A, ... About 38 of the mAb were reactive with myelomonocytic cells, resulting in nine clusters of interest. Although the exact ... should be helpful in further identifying various populations of myelomonocytic cells and their stages of differentiation. Out ...
... anti-myeloid differentiation antigen Gr-1), or anti-CD3 (anti-CD3 TCR-associated complex). Fc receptors of leukocytes were ... Myelo-monocytic HL60 cells were either not treated (hatched bars) or pretreated with piPLC (0.5 U/ml, 90 min, 37°C; black bars ... This in vitro differentiation induced the expression of a monocyte-specific antigen pattern including the molecules CD14, CD11b ... Myelo-monocytic HL60 cells were incubated in the absence (hatched bars) or the presence (black bars) of inactivated uPA (50 nM ...
T lymphocytes regulate the growth and differentiation of T cells and certain B cells through the release of secreted protein ... IL3 is produced by T lymphocytes and T-cell lymphomas only after stimulation with antigens, mitogens, or chemical activators ... However, IL3 is constitutively expressed in the myelomonocytic leukaemia cell line WEHI-3B. It is thought that the genetic ... It plays an essential role in the final differentiation of B cells into immunoglobulin-secreting cells, as well as inducing ...
... myelomonocytic progenitors, primitive B cell progenitors, and thymocytes. CD135 is also expressed on malignant hematopoietic ... Antigen References 1. Rappold I, et al. 1997. Blood 90:111.. 2. Rosnet O, et al. 1996. Acta Haematol. 95:218.. 3. Rosnet O, et ... CD135 is a 130-160 kD type III tyrosine kinase receptor expressed on CD34+ hematopoietic stem cells, myelomonocytic progenitors ... Tyrosine kinase growth factor receptor involved in the growth and differentiation of primitive hematopoietic cells Ligand/ ...
CD14 is a myelomonocytic differentiation antigen expressed by monocytes, macrophages, and activated granulocytes and is ... The CD14 monocyte differentiation antigen maps to a region encoding growth factors and receptors ... The CD14 monocyte differentiation antigen maps to a region encoding growth factors and receptors ... The CD14 monocyte differentiation antigen maps to a region encoding growth factors and receptors ...
The CD19 antigen (also called B4) is a type I membrane glycoprotein with a molecular weight of 95 kDa. CD19 is involved in the ... It is also found on the surface of follicular dendritic cells, on the early cells of myelomonocytic lineage and on most ... regulation of B lymphocyte development, activation and differentiation. This molecule is expressed on all normal B lymphocytes ...
Chemical-registry-number] 0 / Antigens, CD; 0 / Antigens, CD31; 0 / Antigens, Differentiation, Myelomonocytic; 0 / CD68 antigen ... Chemical-registry-number] 0 / Antigens, CD; 0 / Antigens, Differentiation, Myelomonocytic; 0 / CD68 antigen, human; EC 3.4. ... Chemical-registry-number] 0 / Actins; 0 / Antigens, CD; 0 / Antigens, Differentiation, Myelomonocytic; 0 / CD68 antigen, human ... MeSH-major] Antigens, CD / biosynthesis. Antigens, Differentiation, Myelomonocytic / biosynthesis. Biomarkers, Tumor / ...
  • Differentiation of monocytes to macrophages increased galectin-9, and nonclassic monocytes from hepatitis C virus patients had the highest levels of galectin-9. (bioportfolio.com)
  • Differentiation of monocytes to macrophages increased galectin-9. (bioportfolio.com)
  • The CD14 antigen is found on cells of myelomonocytic lineage and is stronly expressed on monocytes and macrophages. (polysciences.com)
  • Siglec F is expressed mostly on eosinophils and alveolar macrophages and lower levels of this receptor have also been reported on immature myelomonocytic cells. (fishersci.com)
  • CD14 is a myelomonocytic differentiation antigen expressed by monocytes, macrophages, and activated granulocytes and is detectable with the monoclonal antibodies MO2, MY4, and LeuM3. (sciencemag.org)
  • en] BACKGROUND: Drug-induced toxic epidermal necrolysis (TEN) probably results from a complex and specific immune cell reaction involving lymphocytes and macrophages. (uliege.be)
  • In particular, the development of protocols for the differentiation of populations of leukocytes as diverse as naïve T cells, macrophages, and natural killer cells provides opportunities for their scale-up and quality control prior to administration. (frontiersin.org)
  • As IgE/antigen immune complexes (IgE-IC) bind to human cells through FcεRI or FcεRII/CD23 surface molecules, the present study aimed to identify which functional receptor may be involved in IgE-IC interaction with human macrophages, the major effector cell during parasite infection. (semanticscholar.org)
  • The killing of Leishmania major by human macrophages is mediated by nitric oxide induced after ligation of the Fc epsilon RII/CD23 surface antigen. (semanticscholar.org)
  • In rheumatoid arthritis, CD163 is used as a marker for the differentiation between synovial macrophages and synovial intimal fibroblasts. (miltenyibiotec.com)
  • Although unlikely to be the 'initiators' of RA (if not as antigen-presenting cells in early disease), macrophages possess widespread pro-inflammatory, destructive, and remodeling capabilities that can critically contribute to acute and chronic disease. (biomedcentral.com)
  • It is unlikely that macrophages occupy a causal position in the pathogenesis of RA (except for their function as antigen-presenting cells in the hypothesis of a primary autoimmune disorder) [ 5 ]. (biomedcentral.com)
  • Thus, even before the cause of RA is identified [whether it is an infectious cause, or a clearly defined (auto)antigen or a genetic alteration (or several)], several facts render monocytes/macrophages attractive therapeutic targets. (biomedcentral.com)
  • Cells of the myelomonocytic lineage differentiate into several cell types that are involved in disease (ie monocytes/macrophages, osteoclasts and dendritic cells). (biomedcentral.com)
  • In the RA synovial membrane, a first differentiation step is that from recently immigrated monocytes to mature macrophages [ 16 ]. (biomedcentral.com)
  • These molecules are constitutively expressed on antigen-presenting cells (APC): dendritic cells (DCs), B lymphocytes, and macrophages [18]. (termedia.pl)
  • CD14 is a myelomonocytic differentiation antigen preferentially expressed on monocytes, macrophages, and activated granulocytes. (proteinkinase.biz)
  • Interleukin-3 (IL3) is a cytokine that regulates blood-cell production by controlling the production, differentiation and function of granulocytes and macrophages [1,2]. (130.88.97)
  • It is expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation. (fishersci.com)
  • MNDA (myeloid cell nuclear differentiation antigen) is a 55kDa nuclear protein constitutively expressed in myelomonocytic leukemia cells, myelomonocytic cell lines, and normal peripheral blood granulocytes and monocytes. (euromabnet.com)
  • In conclusion, flow cytometry in MDS identified aberrancies in the myelomonocytic lineage not otherwise determined by cytomorphology. (bloodjournal.org)
  • Using flow cytometry and Western blotting assays, we determined the effects of FICZ on RA-induced differentiation of HL-60 human leukemia cells. (biomedcentral.com)
  • The typical expression pattern of myelomonocytic differentiation antigens and cytokine receptors in CD4 + AML was CD34 low CD33 high CD11b high GM-CSFR high . (nature.com)
  • While the TCR receptors on T cells react with peptide antigens in the context of major histocompatibility complex (MHC) class I or II molecules, the NKT cells, with use of their TCR receptors, recognise the lipid and glycolipid antigens presented by non-polymorphic MHC class I-like glycoprotein, known as CD1d (Fig. 1) [15]. (termedia.pl)
  • Non-peptide antigen presentation to T-cell receptors on NKT cells, marks T-cells at the short cortical thymic stage of differentiation. (abcam.co.jp)
  • Non peptide antigen presentation to T-cell receptors on NKT cells. (abcam.co.jp)
  • Immature cells of the myelomonocytic lineage were shown to express two distinct molecules: one with an m.w. of 26 to 28 Kd identified by antibody SG133, and the second, a 130 to 140 Kd glycoprotein identified by MoU26. (jimmunol.org)
  • Expression of the CD11b subunit is restricted to cells of the myelomonocytic lineage and depends upon the stage of differentiation with the most mature myeloid cells expressing the highest levels of CD11b. (pnas.org)
  • Our analysis of bone marrow samples from 50 patients with MDS showed aberrant expression of differentiation antigens in the myelomonocytic lineage. (bloodjournal.org)
  • About 65 monoclonal antibodies (mAb) including 17 internal controls were analyzed for their ability to recognize and bind to various cells of the myelomonocytic lineage. (nih.gov)
  • It is also found on the surface of follicular dendritic cells, on the early cells of myelomonocytic lineage and on most stabilized B cell lines. (beckman.com)
  • Transcription factors play a major role in hematopoietic lineage determination and differentiation. (bloodjournal.org)
  • Through their unrivaled capacity for antigen processing and presentation, dendritic cells (DCs) are uniquely equipped to engage naïve T cells in dialog, implicating them in the genesis of all immune responses ( 1 ). (frontiersin.org)
  • Dendritic cells (DCs) are antigen-presenting cells derived from bone marrow precursors and form a widely distributed cellular system throughout the body. (intechopen.com)
  • In addition, markers recently developed for PDCs, including CD123 (the interleukin-3 receptor), blood dendritic cell antigen 2 (BDCA2/CD303), (5) and T-cell leukemia/lymphoma 1 (TCL1) and CD2-associated protein (CD2AP), (6) were rarely tested in the literature published prior to 2008. (thefreelibrary.com)
  • Leukemic cells of patients with chronic myelogenous leukemia (CML), acute myelogenous leukemia (AML), and chronic myelomonocytic leukemia (CMML) will all undergo substantial differentiation toward dendritic cells (DC) and may be used to drive autologous T cells to acquire anti-leukemic cytotoxicity. (begellhouse.com)
  • follicular dendritic cells are also considered to be antigen presenting cells by some. (healthmatics.info)
  • FOLLICULAR DENDRITIC CELLS are not traditional antigen-presenting cells, but because they hold antigen on their cell surface in the form of IMMUNE COMPLEXES for B-cell recognition they are considered so by some authors. (healthmatics.info)
  • Besides CD33, Siglec-9 was the most highly expressed, particularly on AML cells with features of monocytic differentiation that also expressed Siglecs-5 and -7. (dundee.ac.uk)
  • The literature of formerly called blastic NK-cell lymphoma published prior to 2008 may be heterogeneous because CD56 can be expressed in other hematopoietic lineages, including true NK lymphoma and acute myeloid leukemia with monocytic differentiation. (thefreelibrary.com)
  • In contrast, AML1-ETO had little or no effect on monocytic differentiation. (cf.ac.uk)
  • We studied 113 consecutive patients with hematologic malignancies who received non-T-cell-depleted BMT from human leukocyte antigen (HLA)-matched siblings. (cancernetwork.com)
  • The antibodies were prepared against human leukocyte differentiation antigens. (polysciences.com)
  • Here, a first analysis was performed using selected antibodies not yet included in human leukocyte differentiation antigen workshop 8 (HLDA-8). (fu-berlin.de)
  • Neither intercellular adhesion molecule-1 (ICAM-1)/leukocyte function-associated antigen-1 (LCAM-1) nor neural cell adhesion molecule (NCAM)/NCAM adhesion leads to recruitment of beta 1 integrin adhesion pathways. (ox.ac.uk)
  • Leukocyte immunoglobulin-like receptor subfamily A member 3 (LILR-A3) also known as CD85 antigen-like family member E (CD85e), immunoglobulin-like transcript 6 (ILT-6), and leukocyte immunoglobulin-like receptor 4 (LIR-4) is a protein that in humans is encoded by the LILRA3 gene located within the leukocyte receptor complex on chromosome 19q13.4. (wikipedia.org)
  • however, it is highly homologous to other LILR genes, and can bind human leukocyte antigen (HLA) class I. Therefore, if secreted, the LILRA3 might impair interactions of membrane-bound LILRs (such as LILRB1, an inhibitory receptor expressed on effector and memory CD8 T cells) with their HLA ligands, thus modulating immune reactions and influencing susceptibility to disease. (wikipedia.org)
  • Hematopoietic progenitor cells and interleukin-stimulated endothelium: expansion and differentiation of myeloid precursors. (nih.gov)
  • LSCs possess surface antigen profiles and signal transduction activation profiles which may allow differential targeting as compared with normal hematopoietic stem cells. (frontiersin.org)
  • They promote the development and differentiation of T and B lymphocytes, and hematopoietic cells. (wikipedia.org)
  • CD135 is a 130-160 kD type III tyrosine kinase receptor expressed on CD34 + hematopoietic stem cells, myelomonocytic progenitors, primitive B cell progenitors, and thymocytes. (biolegend.com)
  • CD135, also known as FMS-like tyrosine kinase-3, FLT3, STK-1, and Flk-2, is a growth factor receptor that binds the FLT3 ligand to promote the growth and differentiation of primitive hematopoietic cells. (biolegend.com)
  • Expressed on CD34 + hematopoietic stem cells, myelomonocytic progenitors, primitive B cell progenitors, and thymocytes. (biolegend.com)
  • It is also expressed in chronic myeloid leukemia (CML) and M2 and M3 acute myeloid leukemias (AML) as well as myelomonocytic leukemias, but not in T, B, NK and other non-hematopoietic cells. (acris-antibodies.com)
  • Accumulation of genetic defects in hematopoietic precursors leads to impaired cellular differentiation, increased proliferative potential, unregulated proliferation, and defective apoptosis. (medscape.com)
  • CD11b is a relatively late marker for myeloid differentiation, and is undetectable on most myelomonocytic hematopoietic progenitor cells and more primitive cells. (stemcell.com)
  • However, IL3 is constitutively expressed in the myelomonocytic leukaemia cell line WEHI-3B [2]. (130.88.97)
  • The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. (cancerindex.org)
  • However, CD4 + CD34 high AML cases appear to have unique immature characteristics including low expression of myelomonocytic differentiation antigens (ie CD33 and CD11b), and accumulation of chromosome abnormalities (ie t(8;21) in CD4 low CD34 high AML and chromosome 7 abnormalities in CD4 high CD34 high AML). (nature.com)
  • Detailed characterization of the CD11b promoter sequence should provide insight into the molecular events regulating the tissue-specific and developmental stage-specific expression of the CD11b molecule in myelomonocytic cells. (pnas.org)
  • Phenotypic examination of AML1-ETO cultures revealed a defect in granulocytic differentiation in terms of retention of CD34(+) cells within the culture and delayed CD11b upregulation. (cf.ac.uk)
  • Biochemistry and expression of myelomonocytic antigens. (jimmunol.org)
  • By using COS-7 cells transfected with a cDNA clone encoding the MoS39 antigen, various well-described anti-monocyte MAb, including Mo2, My4, Leu-M3 (MoP9), MoP15, MoS1, and 63D3, also bound to MoS39-expressing COS-7 cells, suggesting that this group of antibodies reacted with the same glycoprotein. (jimmunol.org)
  • The CD19 antigen (also called B4) is a type I membrane glycoprotein with a molecular weight of 95 kDa. (beckman.com)
  • Cluster of differentiation 14 (CD14) , is a cell surface glycoprotein, and is a is a component of the innate immune system. (proteinkinase.biz)
  • Surface antigens expressed on myeloid cells of the granulocyte-monocyte-histiocyte series during differentiation. (harvard.edu)
  • HCV-infected cells and differentiation increase monocyte immunoregulatory galectin-9 production. (bioportfolio.com)
  • PU.1 is highly expressed in B and myelomonocytic cells, 3 and in their precursors such as pro-B cells and granulocyte/monocyte progenitors (GMPs). (bloodjournal.org)
  • Analysis of their reactivity in normal and malignant myelomonocytic cells is useful in identifying and classifying human leukemias and lymphomas. (harvard.edu)
  • In this review, we will focus on acute leukemias where impairment of differentiation is found. (frontiersin.org)
  • During microbial infections and in myelomonocytic leukemias CD163 is expressed in a higher level. (miltenyibiotec.com)
  • But RA is not effective for other myeloid leukemias, making the mechanism of RA-induced differentiation observed in a non-APL myeloid leukemia of interest. (biomedcentral.com)
  • Phagoburst results are shown in response to PMA, fMLP and E. coli of HPC expanded in IL1-stimulated EC and following further differentiation by G-CSF in comparison to granulocytes differentiated by cytokines alone. (nih.gov)
  • BM-1 antigen is a 183 kD myeloid-specific-DNA-binding protein which is expressed in myeloid cells, including myeloid precursors and mature granulocytes. (acris-antibodies.com)
  • Morphologically, granulocyte terminal differentiation in AML1-ETO-expressing cells was inhibited by 83+/-5%, giving rise to a build-up of early to intermediate granulocytes that exhibited a number of morphological features associated with t(8;21) leukaemias. (cf.ac.uk)
  • The potentially relevant genes that were elevated (15% to 28%) were P protein, LCK, cAMP responsive element modulator, IL-7 receptor, matrix metalloproteinase-19, M130 antigen, and peptidyl-prolyl isomerase. (nih.gov)
  • The name "interleukin" was chosen in 1979, to replace the various different names used by different research groups to designate interleukin 1 (lymphocyte activating factor, mitogenic protein, T-cell replacing factor III, B-cell activating factor, B-cell differentiation factor, and "Heidikine") and interleukin 2 (TSF, etc. (wikipedia.org)
  • T lymphocytes regulate the growth and differentiation of T cells and certain B cells through the release of secreted protein factors. (wikipedia.org)
  • Clone REAL406 recognizes the human CD163 antigen, a single-chain transmembrane protein also known as hemoglobin scavenger receptor or M130. (miltenyibiotec.com)
  • In gene-set enrichment analysis, the IDO pathway revealed a significant (false discovery rate (FDR) = 0.07) regulatory T cell, fork-head box protein 3 (FoxP3)-initiated CD28-cytotoxic T lymphocyte-associated antigen 4 (CTLA-4)-inducible T cell co-stimulator (ICOS)-driven pathway leading to activation of IDO expression in antigen-presenting cells (APCs). (arctichealth.org)
  • S100A8 and S100A9 protein localization were determined in antigen-induced inflammatory arthritis in mice, mouse femoral head cartilage explants stimulated with interleukin-1 (IL-1), and in surgically-induced OA in mice. (biomedcentral.com)
  • FICZ causes changes in signaling events that are known to drive differentiation, and notably augments the RA-induced sustained activation of the RAF/MEK/ERK axis of the mitogen-activated protein kinase (MAPK) cascade. (biomedcentral.com)
  • In contrast, IL-1beta and IL-3 stimulation resulted in a 10- and 100-fold increase in cell numbers with more than 90% of these cells being CD33(+).Fewer 2-week cobblestones and greater amounts of 5-week cobblestones were observed with IL-6 and IL-3.IL-1beta and IL-3 stimulated endothelium induces proliferation and differentiation of myeloid precursors, while IL-6 treatment induced a benefit of HPC survival. (nih.gov)
  • IL-1beta and IL-3 stimulated endothelium induces proliferation and differentiation of myeloid precursors, while IL-6 treatment induced a benefit of HPC survival. (nih.gov)
  • Precursors of DCs harbor latent infection, and once terminally differentiated into mature antigen-presenting cells, they support reactivation and viral persistent replication (11, 12). (acancerjourney.info)
  • 2,3 Stem cells are capable of self-renewal, transformation into dedicated progenitor cells, and differentiation into specialized progeny. (ahajournals.org)
  • and the functional differentiation marker, inducible-oxidative metabolism. (biomedcentral.com)
  • The complex formed by the binding of antigen and antibody molecules. (healthmatics.info)
  • The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES. (healthmatics.info)
  • MSH immune response induced by binding antigen to antibody. (healthmatics.info)
  • Six monoclonal antibodies (MAb) which react with myelomonocytic cells representing various stages of differentiation, and which precipitate six different cell surface molecules, were identified. (jimmunol.org)
  • Myelodysplastic syndromes (MDSs) represent a heterogeneous group of myeloid neoplasms characterized by abnormal differentiation and maturation of myeloid cells, bone marrow failure, and a genetic instability with enhanced risk to transform to acute myeloid leukemia (AML). (bloodjournal.org)
  • In the present study, the effects of rSj16 on the differentiation of the murine myeloid leukemia WEHI-3B JCS cell line and on mouse hematopoiesis were investigated. (hindawi.com)
  • Cutaneous infiltration by neoplastic lymphocytes may be seen in acute myeloid leukemia , acute lymphocytic leukemia , chronic myeloid leukemia , chronic lymphoid leukemia , hairy cell leukemia, prolymphocytic leukemia, chronic myelomonocytic leukemia, and myelodysplastic syndromes . (medscape.com)
  • The macrophage differentiation of the rSj16-treated WEHI-3B JCS cells was confirmed by their expression of macrophage specific antigen F4/80 and phagocytic activity. (hindawi.com)
  • DCs exert immune-surveillance for exogenous and endogenous antigens and the later activation of naive T lymphocytes giving rise to various immunological responses. (intechopen.com)
  • The NKT cells can react with both endogenous and exogenous antigens, regulating a variety of autoimmune diseases, but also providing protection against different pathogens like Sphingomonas spp. (termedia.pl)
  • Antigens, Differentiation, Myelomonocytic" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
  • Most therapies available to treat acute myelogenous leukemia target leukemia blast cells which are in general a later progenitor/precursor cell no longer capable of self-renewal and multipotential differentiation. (frontiersin.org)
  • The cell line WEHI-3B JCS is a D-subclone isolated from the WEHI 3B myelomonocytic leukemia cell line [ 2 ]. (hindawi.com)
  • 4) The etiology of BPDCN is not, but it has been suggested to be associated with acute myeloid/myelomonocytic leukemia (Petrella et al. (thefreelibrary.com)
  • We recently reported that AhR promotes retinoic acid (RA)-induced granulocytic differentiation of HL-60 myeloblastic leukemia cells by restricting the nuclear abundance of the stem cell associated transcription factor Oct4. (biomedcentral.com)
  • The standard clinical management of acute promyelocytic leukemia (APL) is differentiation induction therapy using RA. (biomedcentral.com)
  • IL3 is produced by T-lymphocytes and T-lymphomas only after stimulation with antigens, mitogens, or chemical activators such as phorbol esters. (130.88.97)
  • A type of immune cell that boosts immune responses by showing antigens on its surface to other cells of the immune system. (healthmatics.info)
  • A cell that enables a T-lymphocyte to recognize an antigen by engulfing the antigen, breaking down the antigen into smaller fragments which bind to MHC molecules on the surface of the antigen presenting cell. (healthmatics.info)
  • Change in the surface ANTIGEN of a microorganism. (healthmatics.info)
  • Co-ligation of the CD19-CD21-CD81 complex with the surface IgM-B-cell antigen receptor (BCR) leads to the phosphorylation of CD19 by Syk, followed by the recruitment of positive signal transduction effectors such as phosphatidylinositol 3 kinase (PI3 kinase), Lyn, and Fyn. (mybeckman.ca)
  • Conversely, 11beta-HSD1 mRNA together with the markers of late adipocyte differentiation (FABP4 and G3PDH) were significantly lower in OF. (ox.ac.uk)
  • Further studies indicated that rSj16 induced macrophage differentiation of the WEHI-3B JCS cells, and arrested the cell cycle in the G1/G0 and G2/M phases. (hindawi.com)
  • Enforced PU.1 in a multipotent myeloid cell line induced macrophage differentiation. (bloodjournal.org)
  • 18 In primary fetal liver progenitors, the introduction of PU.1 at a high level induced macrophage differentiation, whereas a low level of PU.1 induced B-cell differentiation. (bloodjournal.org)
  • Here, we describe protocols for the directed differentiation of human iPSCs into a mixed population of CD11c + DCs through the spontaneous formation of embryoid bodies and exposure to a cocktail of growth factors, the scheduled withdrawal of which serves to guide the process of differentiation. (frontiersin.org)
  • The T-lymphocyte can now recognize and bind with the MHC-linked antigen. (healthmatics.info)
  • The processes triggered by interactions of ANTIBODIES with their ANTIGENS. (healthmatics.info)
  • Sites on an antigen that interact with specific antibodies. (healthmatics.info)
  • Most patients developed KLH antibodies supporting our hypothesis that the co-injected iDC are functional with the capacity to acquire antigens from their environment and generate an adaptive immune response. (mdpi.com)
  • In bulk liquid culture, we found that though AML1-ETO transiently inhibited the proliferation of CD34(+) cells, it promoted long-term growth of myeloid cells for more than 80 days, suggesting that differentiation was inhibited. (cf.ac.uk)
  • Although the exact identity of many of the molecules on the cells bound by the mAb remains undetermined, information obtained about the mAb analyzed in this workshop should be helpful in further identifying various populations of myelomonocytic cells and their stages of differentiation. (nih.gov)
  • FICZ also augments expression of the known MAPK signaling regulatory molecules c-Cbl, VAV1, pY458 p85 PI3K, Src-family kinases (SFKs), and IRF-1, a transcription factor associated with this putative signalsome that promotes RA-induced differentiation. (biomedcentral.com)
  • CD19 is involved in the regulation of B lymphocyte development, activation and differentiation. (beckman.com)
  • It is a signal transduction molecule that regulates lymphocyte development, activation, and differentiation. (mybeckman.ca)
  • Types of Cytomegalovirus Latency Cell culture choices possess reinforced the part from the cellular differentiation state in the maintenance of CMV latency-reactivation balance. (acancerjourney.info)
  • As a result of their marked plasticity, these differentiation pathways can be influenced by particular pathophysiological stimuli (eg an excess/imbalance of cytokines or growth factors), resulting in altered differentiation or maturation if regulatory mechanisms fail. (biomedcentral.com)
  • Our data suggest that FICZ modulates intracellular signaling pathways and enhances RA-induced differentiation. (biomedcentral.com)
  • Furthermore, the differentiation pathways that lead to reactivation depend on the inflammatory environment and also likely stimulate the adaptive T-cell response to viral infection. (acancerjourney.info)
  • In the present study, we reported the effects of rSj16 on the proliferation and differentiation of the WEHI-3B JCS and on mouse hematopoiesis. (hindawi.com)
  • Although IHC is not commonly necessary in differentiation, epithelial membrane antigen (EMA) highlights most mature sebocytes in sebaceoma, whereas its expression is uncommon in BCC. (thefreelibrary.com)