Research: Critical and exhaustive investigation or experimentation, having for its aim the discovery of new facts and their correct interpretation, the revision of accepted conclusions, theories, or laws in the light of newly discovered facts, or the practical application of such new or revised conclusions, theories, or laws. (Webster, 3d ed)Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Research Personnel: Those individuals engaged in research.Research Support as Topic: Financial support of research activities.Biomedical Research: Research that involves the application of the natural sciences, especially biology and physiology, to medicine.Biophysics: The study of PHYSICAL PHENOMENA and PHYSICAL PROCESSES as applied to living things.Research Design: A plan for collecting and utilizing data so that desired information can be obtained with sufficient precision or so that an hypothesis can be tested properly.Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Ethics, Research: The moral obligations governing the conduct of research. Used for discussions of research ethics as a general topic.National Institutes of Health (U.S.): An operating division of the US Department of Health and Human Services. It is concerned with the overall planning, promoting, and administering of programs pertaining to health and medical research. Until 1995, it was an agency of the United States PUBLIC HEALTH SERVICE.PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.BooksPublishing: "The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.MEDLINE: The premier bibliographic database of the NATIONAL LIBRARY OF MEDICINE. MEDLINE® (MEDLARS Online) is the primary subset of PUBMED and can be searched on NLM's Web site in PubMed or the NLM Gateway. MEDLINE references are indexed with MEDICAL SUBJECT HEADINGS (MeSH).Serial Publications: Publications in any medium issued in successive parts bearing numerical or chronological designations and intended to be continued indefinitely. (ALA Glossary of Library and Information Science, 1983, p203)Biological Science Disciplines: All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.Abstracting and Indexing as Topic: Activities performed to identify concepts and aspects of published information and research reports.Information Storage and Retrieval: Organized activities related to the storage, location, search, and retrieval of information.Bibliometrics: The use of statistical methods in the analysis of a body of literature to reveal the historical development of subject fields and patterns of authorship, publication, and use. Formerly called statistical bibliography. (from The ALA Glossary of Library and Information Science, 1983)Bile Canaliculi: Minute intercellular channels that occur between liver cells and carry bile towards interlobar bile ducts. Also called bile capillaries.Perna: A genus of freshwater mussel in the family MYTILIDAE, class BIVALVIA. It is found in tropical and warm temperate coastal waters. Most species have green in their shells.Boron: A trace element with the atomic symbol B, atomic number 5, and atomic weight [10.806; 10.821]. Boron-10, an isotope of boron, is used as a neutron absorber in BORON NEUTRON CAPTURE THERAPY.Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.Transcytosis: The transport of materials through a cell. It includes the uptake of materials by the cell (ENDOCYTOSIS), the movement of those materials through the cell, and the subsequent secretion of those materials (EXOCYTOSIS).Copper: A heavy metal trace element with the atomic symbol Cu, atomic number 29, and atomic weight 63.55.Liver Neoplasms, Experimental: Experimentally induced tumors of the LIVER.Bile: An emulsifying agent produced in the LIVER and secreted into the DUODENUM. Its composition includes BILE ACIDS AND SALTS; CHOLESTEROL; and ELECTROLYTES. It aids DIGESTION of fats in the duodenum.Lysosome-Associated Membrane Glycoproteins: Ubiquitously expressed integral membrane glycoproteins found in the LYSOSOME.Lysosomes: A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured. Such rupture is supposed to be under metabolic (hormonal) control. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)Colitis: Inflammation of the COLON section of the large intestine (INTESTINE, LARGE), usually with symptoms such as DIARRHEA (often with blood and mucus), ABDOMINAL PAIN, and FEVER.Dextran Sulfate: Long-chain polymer of glucose containing 17-20% sulfur. It has been used as an anticoagulant and also has been shown to inhibit the binding of HIV-1 to CD4-POSITIVE T-LYMPHOCYTES. It is commonly used as both an experimental and clinical laboratory reagent and has been investigated for use as an antiviral agent, in the treatment of hypolipidemia, and for the prevention of free radical damage, among other applications.Mast Cells: Granulated cells that are found in almost all tissues, most abundantly in the skin and the gastrointestinal tract. Like the BASOPHILS, mast cells contain large amounts of HISTAMINE and HEPARIN. Unlike basophils, mast cells normally remain in the tissues and do not circulate in the blood. Mast cells, derived from the bone marrow stem cells, are regulated by the STEM CELL FACTOR.Colitis, Ulcerative: Inflammation of the COLON that is predominantly confined to the MUCOSA. Its major symptoms include DIARRHEA, rectal BLEEDING, the passage of MUCUS, and ABDOMINAL PAIN.Colon: The segment of LARGE INTESTINE between the CECUM and the RECTUM. It includes the ASCENDING COLON; the TRANSVERSE COLON; the DESCENDING COLON; and the SIGMOID COLON.Trinitrobenzenesulfonic Acid: A reagent that is used to neutralize peptide terminal amino groups.Intestinal Mucosa: Lining of the INTESTINES, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI.Mice, Inbred C57BLCell Degranulation: The process of losing secretory granules (SECRETORY VESICLES). This occurs, for example, in mast cells, basophils, neutrophils, eosinophils, and platelets when secretory products are released from the granules by EXOCYTOSIS.Inflammatory Bowel Diseases: Chronic, non-specific inflammation of the GASTROINTESTINAL TRACT. Etiology may be genetic or environmental. This term includes CROHN DISEASE and ULCERATIVE COLITIS.Publications: Copies of a work or document distributed to the public by sale, rental, lease, or lending. (From ALA Glossary of Library and Information Science, 1983, p181)HungaryAcademies and Institutes: Organizations representing specialized fields which are accepted as authoritative; may be non-governmental, university or an independent research organization, e.g., National Academy of Sciences, Brookings Institution, etc.Databases, Bibliographic: Extensive collections, reputedly complete, of references and citations to books, articles, publications, etc., generally on a single subject or specialized subject area. Databases can operate through automated files, libraries, or computer disks. The concept should be differentiated from DATABASES, FACTUAL which is used for collections of data and facts apart from bibliographic references to them.

Altered trafficking of lysosomal proteins in Hermansky-Pudlak syndrome due to mutations in the beta 3A subunit of the AP-3 adaptor. (1/236)

Hermansky-Pudlak syndrome (HPS) is a genetic disorder characterized by defective lysosome-related organelles. Here, we report the identification of two HPS patients with mutations in the beta 3A subunit of the heterotetrameric AP-3 complex. The patients' fibroblasts exhibit drastically reduced levels of AP-3 due to enhanced degradation of mutant beta 3A. The AP-3 deficiency results in increased surface expression of the lysosomal membrane proteins CD63, lamp-1, and lamp-2, but not of nonlysosomal proteins. These differential effects are consistent with the preferential interaction of the AP-3 mu 3A subunit with tyrosine-based signals involved in lysosomal targeting. Our results suggest that AP-3 functions in protein sorting to lysosomes and provide an example of a human disease in which altered trafficking of integral membrane proteins is due to mutations in a component of the sorting machinery.  (+info)

PETA-3/CD151, a member of the transmembrane 4 superfamily, is localised to the plasma membrane and endocytic system of endothelial cells, associates with multiple integrins and modulates cell function. (2/236)

The Transmembrane 4 Superfamily member, PETA-3/CD151, is ubiquitously expressed by endothelial cells in vivo. In cultured human umbilical vein endothelial cells PETA-3 is present on the plasma membrane and predominantly localises to regions of cell-cell contact. Additionally, this protein is abundant within an intracellular compartment which accounts for up to 66% of the total PETA-3 expressed. Intracellular PETA-3 showed colocalisation with transferrin receptor and CD63 suggesting an endosomal/lysosomal localisation which was supported by immuno-electronmicroscopy studies. Co-immunoprecipitation experiments investigating possible interactions of PETA-3 with other molecules demonstrated associations with several integrin chains including beta1, beta3, beta4, (alpha)2, (alpha)3, (alpha)5, (alpha)6 and provide the first report of Transmembrane 4 Superfamily association with the (alpha)6beta4 integrin. Using 2-colour confocal microscopy, we demonstrated similar localisation of PETA-3 and integrin chains within cytoplasmic vesicles and endothelial cell junctions. In order to assess the functional implications of PETA-3/integrin associations, the effect of anti-PETA-3 antibodies on endothelial function was examined. Anti-PETA-3 mAb inhibited endothelial cell migration and modulated in vitro angiogenesis, but had no detectable effect on neutrophil transendothelial migration. The broad range of integrin associations and the presence of PETA-3 with integrins both on the plasma membrane and within intracellular vesicles, suggests a primary role for PETA-3 in regulating integrin trafficking and/or function.  (+info)

Role of autocrine stimulation on the effects of cyclic AMP on protein and lipid phosphorylation in collagen-activated and thrombin-activated platelets. (3/236)

We compared several responses in thrombin-stimulated and collagen (type I)-stimulated platelets with and without forskolin and inhibitors of autocrine stimulation (IAS: an ADP-removing system of creatine phosphate/creatine phosphokinase, Arg-Gly-Asp-Ser peptide to prevent fibrinogen/fibronectin binding to GPIIb/IIIa, SQ 29.548 as a thromboxane A2 receptor antagonist, cyproheptadine as a serotonin receptor antagonist, BN 52021 as a platelet-activating factor receptor antagonist). The pattern of tyrosine-phosphorylated proteins, the phosphorylation of lipids in the polyphosphoinositide cycle and phosphorylation of pleckstrin (P47) were studied as markers for signal-transducing responses, exposure of CD62 (P-selectin) and CD63 (Glycoprotein 53), as well as secretion of ADP + ATP and beta-N-acetyl-glycosaminidase were studied as final activation responses. Clear differences between thrombin-stimulated and collagen-stimulated platelets were observed. First, practically all protein-tyrosine phosphorylation induced by thrombin was inhibited by IAS, while a partial inhibition was observed for collagen; the phosphorylation due to collagen alone was apparently stimulated by elevation of cAMP. Secondly, the other responses to thrombin were inhibited by increased levels of cAMP, independent of autocrine stimulation. In contrast, only the autocrine part of the collagen-induced responses was inhibited by elevation of cAMP. Thus, the inhibition by elevated cAMP seen in collagen-stimulated platelets seems to be due to removal of the G-protein-mediated activation from secreted autocrine stimulators either by IAS or forskolin. The remaining activity is a pure collagen effect which is not affected by elevated levels of cAMP.  (+info)

Detection of allergen-induced basophil activation by expression of CD63 antigen using a tricolour flow cytometric method. (4/236)

In the field of allergy diagnosis, most in vitro functional tests are focused on basophils. Nevertheless, the very small number of circulating basophils limits these experiments and their clinical benefit remains controversial. As flow cytometry is a valuable tool for identifying cell populations, even at low concentrations, we developed a tricolour flow cytometric method for the study of allergen-induced basophil activation. Identification of cells was based both on CD45 expression and on the presence of IgE on the cell surface, since basophils express high-affinity receptors for IgE (Fc epsilon RI). Cell activation upon allergen challenge was assessed by the expression of CD63 antigen on the plasma membrane. Basophil isolation and activation (with the chemotactic peptide formyl-methionyl-leucyl-phenylalanine) were validated in 32 non-allergic patients. In 12 allergic patients, basophil stimulation by a relevant allergen was in most cases positive (10/12). Furthermore a concentration-dependent hook effect was observed. Of the allergic and non-allergic patients, none showed non-specific activation with an irrelevant allergen (specificity 100%). Overall, our preliminary results, even in a small population, suggest that this is a reliable and valuable method for the diagnosis of allergies complementing specific allergen IgE and skin test results. Obviously, additional clinical studies are needed to validate these first results.  (+info)

Rapid down-regulation of CD63 transcription by progesterone in human endometrial stromal cells. (5/236)

Differentiation of endometrial stromal cells (decidualization) plays a crucial role in embryo implantation and maintenance of pregnancy. While progesterone is a key factor in regulating endometrial cell decidualization, the molecular mechanisms remain unclear. In the present study, we investigated the effect of gene transcription in human endometrial stromal cells (ESC) by progesterone, oestrogen or vehicle using the polymerase chain reaction-based differential display methodology. A transcript which is down-regulated by progesterone, but not by vehicle and oestrogen, was identified from a differential display band and the progesterone sensitivity of its expression was verified in Northern blot analysis. The level of the gene expression in progesterone-treated ESC was approximately 60% of that in the vehicle- and oestrogen-treated ESC. This cDNA was revealed to be human CD63 antigen, a recently identified member of the transmembrane 4 superfamily. The inhibitory effect of progesterone is observed within 30 min after hormone treatment. In human endometrium, CD63 mRNA levels were significantly decreased (P < 0.05) during the secretory phase compared with levels during the proliferative phase. This down-regulation of CD63 in vivo elevated levels of progesterone in the secretory phase. These results suggest that CD63 transcription is down-regulated by progesterone in human endometrium.  (+info)

Monocyte activation in patients with Wegener's granulomatosis. (6/236)

OBJECTIVE: Wegener's granulomatosis (WG) is an inflammatory disorder characterised by granulomatous inflammation, vasculitis, and necrotising vasculitis and is strongly associated with anti-neutrophil cytoplasmic antibodies (ANCA). Activated monocytes/macrophages are present in renal biopsy specimens and participate in granuloma formation by synthesising and secreting a variety of chemoattractants, growth factors, and cytokines. In view of these findings, in vivo monocyte activation was evaluated in patients with WG and the findings related to parameters of clinical disease activity. METHODS: Monocyte activation was analysed by measuring plasma concentrations of soluble products of monocyte activation, that is neopterin and interleukin 6 (IL6), by ELISA, and by quantitating the surface expression of activation markers on circulating monocytes by flow cytometry. RESULTS: Twenty-four patients with active WG were included in this study. Ten of these patients were also analysed at the time of remission. Twelve patients with sepsis served as positive controls, and 10 healthy volunteers as negative controls for monocyte activation. Patients with active disease had increased monocyte activation compared with healthy controls as shown by increased concentrations of neopterin (p < 0.0001) and increased surface expression of CD11b (p < 0.05) and CD64 (p < 0.05). In those patients with increased concentrations of IL6 during active disease plasma concentrations of IL6 decreased during follow up when patients went into remission (p < 0.0001). In addition, neopterin (r = 0.37, r = 0.44), IL6 (r = 0.37, r = 0.60) and CD63 expression (r = 0.39, r = 0.45) correlated significantly with disease activity as measured by the Birmingham Vasculitis Activity Score and C reactive protein values, respectively. Compared with patients with sepsis, all markers of monocyte activation in patients with vasculitis were lower. CONCLUSION: It is concluded that disease activity in WG correlates with the extent of activation of monocytes, compatible with their role in the pathophysiology of this disease.  (+info)

Y receptor-mediated induction of CD63 transcripts, a tetraspanin determined to be necessary for differentiation of the intestinal epithelial cell line, hBRIE 380i cells. (7/236)

Peptide YY (PYY) and neuropeptide Y (NPY) are peptides that coordinate intestinal activities in response to luminal and neuronal signals. In this study, using the rat hybrid small intestinal epithelial cell line, hBRIE 380i cells, we demonstrated that PYY- and NPY-induced rearrangement of actin filaments may be in part through a Y1alpha and/or a nonneuronal Y2 receptor, which were cloned from both the intestinal mucosa and the hBRIE 380i cells. A number of PYY/NPY-responsive genes were also identified by subtractive hybridization of the hBRIE 380i cells in the presence or absence of a 6-h treatment with PYY. Several of these genes coded for proteins associated with the cell cytoskeleton or extracellular matrix. One of these proteins was the transmembrane-4 superfamily protein CD63, previously shown to associate with beta(1)-integrin and implicated in cell adhesion. CD63 immunoreactivity, using antibody to the extracellular domain, was highest in the differentiated cell clusters of the hBRIE 380i cells. The hBRIE 380i cells transfected with antisense CD63 cDNA lost these differentiated clusters. These studies suggest a new role for NPY and PYY in modulating differentiation through cytoskeletal associated proteins.  (+info)

CD63 associates with CD11/CD18 in large detergent-resistant complexes after translocation to the cell surface in human neutrophils. (8/236)

CD63 antibody binding to the neutrophil surface triggers a transient activation signal that regulates the adhesive activity and surface expression of CD11/CD18. Gel permeation chromatography demonstrated that all of the cell surface CD11/CD18 associated with CD63 eluted in the void volume, indicating that they were present in large detergent-resistant complexes. In contrast, the majority of the total cellular CD63, CD11 and CD18, which are largely intracellular, was not present in complexes. The data suggest that intracellular CD11, CD18 and CD63 are not in detergent-resistant complexes, but enter such complexes following translocation to the cell surface.  (+info)

*CD63

... antigen is a protein that in humans is encoded by the CD63 gene. CD63 is mainly associated with membranes of intracellular ... 1991). "CD63 antigen. A novel lysosomal membrane glycoprotein, cloned by a screening procedure for intracellular antigens in ... In cell biology, CD63 is often used as a marker for multivessicular bodies, which are enriched with CD63. CD63 has been shown ... CD63 protein, human at the US National Library of Medicine Medical Subject Headings (MeSH) Human CD63 genome location and CD63 ...

*Basophil activation

The CD63 marker is an FITC labeled antigen which can bind to an CD63 protein and is used to sort the cells via FACS( ... the expression of the CD63 antigen on the cell surface (plasma membrane) allows identification of the allergen responsible for ... After degranulation a CD63 marker (labeld antibodies) is added to the test tube. Several minutes at room temperature gives the ... It can be used for different allergies (e.g. bee venom, drugs, contrast media). Degranulated cell expose CD63 molecules on ...

*CD82 (gene)

1992). "C33 antigen recognized by monoclonal antibodies inhibitory to human T cell leukemia virus type 1-induced syncytium ... CD82 (gene) has been shown to interact with CD19, CD63 and CD234 Cluster of differentiation GRCh38: Ensembl release 89: ... 1991). "A new superfamily of lymphoid and melanoma cell proteins with extensive homology to Schistosoma mansoni antigen Sm23". ... 1996). "Transmembrane-4 superfamily proteins CD81 (TAPA-1), CD82, CD63, and CD53 specifically associated with integrin alpha 4 ...

*CD49d

... antigen at the US National Library of Medicine Medical Subject Headings (MeSH) ITGA4 Info with links in the Cell ... CD63, and CD53 specifically associated with integrin alpha 4 beta 1 (CD49d/CD29)". J. Immunol. 157 (5): 2039-47. PMID 8757325. ... Takada Y, Strominger JL, Hemler ME (1987). "The very late antigen family of heterodimers is part of a superfamily of molecules ... antigen CD49D, alpha 4 subunit of VLA-4 receptor)". Hadari YR, Arbel-Goren R, Levy Y, Amsterdam A, Alon R, Zakut R, Zick Y ( ...

*CD37

"The primary structure of the human leukocyte antigen CD37, a species homologue of the rat MRC OX-44 antigen". The Journal of ... CD53 and CD63". FEBS Letters. 288 (1-2): 1-4. doi:10.1016/0014-5793(91)80988-F. PMID 1879540. Berditchevski F (December 2001 ... Leukocyte antigen CD37 is a protein that in humans is encoded by the CD37 gene. The protein encoded by this gene is a member of ... Angelisová P, Hilgert I, Horejsí V (1994). "Association of four antigens of the tetraspans family (CD37, CD53, TAPA-1, and R2/ ...

*CD9

... antigen is a protein that in humans is encoded by the CD9 gene. The protein encoded by this gene is a member of the ... Radford KJ, Thorne RF, Hersey P (May 1996). "CD63 associates with transmembrane 4 superfamily members, CD9 and CD81, and with ... Boucheix C, Benoit P, Frachet P, Billard M, Worthington RE, Gagnon J, Uzan G (1991). "Molecular cloning of the CD9 antigen. A ... Radford KJ, Thorne RF, Hersey P (1996). "CD63 associates with transmembrane 4 superfamily members, CD9 and CD81, and with beta ...

*CD81

Radford KJ, Thorne RF, Hersey P (May 1996). "CD63 associates with transmembrane 4 superfamily members, CD9 and CD81, and with ... 1994). "Mouse homologue of C33 antigen (CD82), a member of the transmembrane 4 superfamily: complementary DNA, genomic ... Radford KJ, Thorne RF, Hersey P (1996). "CD63 associates with transmembrane 4 superfamily members, CD9 and CD81, and with beta ... The tetraspanin family includes CD9, CD37, CD53, CD63, CD81 (this protein), CD82 and CD151. CD81 interacts directly with ...

*CD53

1990). "The human leucocyte surface antigen CD53 is a protein structurally similar to the CD37 and MRC OX-44 antigens". ... 1998). "Expression of tetra-spans transmembrane family (CD9, CD37, CD53, CD63, CD81 and CD82) in normal and neoplastic human ... Leukocyte surface antigen CD53 is a protein that in humans is encoded by the CD53 gene. The protein encoded by this gene is a ... A pan-leukocyte antigen related to membrane transport proteins". J. Immunol. 145 (12): 4322-5. PMID 2258620. Dianzani U, ...

*Basophil

Recently, Heneberg proposed that basophils may be defined as the cellular population positive for CD13, CD44, CD54, CD63, CD69 ... pollen proteins or helminth antigens. Recent studies in mice suggest that basophils may also regulate the behavior of T cells ... When activated, some additional surface markers are known to be upregulated (CD13, CD107a, CD164), or surface-exposed (CD63, ...

*CD151

Raph blood group system in the BGMUT blood group antigen gene mutation database Human CD151 genome location and CD151 gene ... CD63, and alpha5beta1 integrin". J. Histochem. Cytochem. 45 (4): 515-25. doi:10.1177/002215549704500404. PMID 9111230. Suzuki Y ... identifies a novel platelet surface antigen". Br. J. Haematol. 79 (2): 263-70. doi:10.1111/j.1365-2141.1991.tb04531.x. PMID ... "Molecular cloning of cDNA encoding a novel platelet-endothelial cell tetra-span antigen, PETA-3". Blood. 86 (4): 1348-55. PMID ...

*TSPAN8

"Molecular cloning of cDNA for the human tumor-associated antigen CO-029 and identification of related transmembrane antigens". ... Gwynn B, Eicher EM, Peters LL (July 1996). "Genetic localization of Cd63, a member of the transmembrane 4 superfamily, reveals ...

*PI4KA

Subrahmanyam G, Rudd CE, Schneider H (2003). "Association of T cell antigen CD7 with type II phosphatidylinositol-4 kinase, a ... Israels SJ, McMillan-Ward EM (2005). "CD63 modulates spreading and tyrosine phosphorylation of platelets on immobilized ... Israels SJ, McMillan-Ward EM (2005). "CD63 modulates spreading and tyrosine phosphorylation of platelets on immobilized ...

*CD29

... Antigen at the US National Library of Medicine Medical Subject Headings (MeSH) Human ITGB1 genome location and ITGB1 gene ... Radford KJ, Thorne RF, Hersey P (May 1996). "CD63 associates with transmembrane 4 superfamily members, CD9 and CD81, and with ... CD29 is an integrin unit associated with very late antigen receptors. It is known to conjoin with alpha-3 subunit to create ... "Entrez Gene: ITGB1 integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)". Hynes RO (Apr ...

*Alain de Weck

2002). "Flow cytometric basophil activation test by detection of CD63 expression in patients with immediate-type reactions to ... leukocyte histocompatibility antigen (HLA group) typing for organ transplant (mainly kidney and liver), as well as cellular ...

*CD117

... has been shown to interact with: APS, BCR, CD63, CD81, CD9, CRK, CRKL, DOK1, FES, GRB10, Grb2, KITLG, LNK, LYN, MATK, ... Ashman LK, Cambareri AC, To LB, Levinsky RJ, Juttner CA (1991). "Expression of the YB5.B8 antigen (c-kit proto-oncogene product ... "Signal transduction-associated and cell activation-linked antigens expressed in human mast cells". Int. J. Hematol. 75 (4): 357 ...
Two lines of evidence in our current study suggest that surface TEMs can serve as exit gateways for HIV-1. First, most cell surface punctae where either Gag or Env clusters in both HeLa cell and in Jurkat T lymphocytes are also occupied by one of the tetraspanins (Figs. 6-8⇑⇑ and 10). Second, cellular TSG101 and VPS28, the components of the cellular budding machinery responsible for viral egress (Morita and Sundquist, 2004), when recruited to the plasma membrane in cells producing HIV-1, accumulate at CD63-containing TEMs (Fig. 8). Furthermore, we find that distortion of TEM distribution in virus-producing cells by an anti-CD9 antibody (K41) correlates with inhibition of HIV-1 release (unpublished data).. In a study where we used the FlAsH technique for successive dual-color labeling (Gaietta et al., 2002) of Gag in various virus-secreting cell types, we observed localization of newly synthesized Gag at distinct areas on the plasma membrane (Rudner et al., 2005). We also documented that ...
Basophil activation in unfractionated samples such as whole blood can be measured by changes in the expression of cell surface markers or even intracellular events (e.g. phosphorylation, oxidative burst, calcium flux) by flow cytometry. Separate markers (e.g. as CD123+ HLA-DR-) are used to specifically identify basophils in addition to those used for assessment of activation. A feature of this protocol is to stimulate the basophils both in the presence and absence of autologous plasma. This protocol is specifically for a mouse allergen SCIT study conducted at JHU. ...
Basophil activation in unfractionated samples such as PBMC (note basophils are less dense than Ficoll) or whole blood can be measured by changes in the expression of cell surface markers or even intracellular events (e.g. phosphorylation, oxidative burst, calcium flux) by flow cytometry. Separate markers (e.g. as CD123+ HLA-DR-) are used to specifically identify basophils in addition to those used for assessment of activation. This protocol is specifically for the JAX study. ...
A change in morphology and behavior of a basophil resulting from exposure to a cytokine, chemokine, soluble factor, or to (at least in mammals) an antigen which the basophil has specifically bound via IgE bound to Fc-epsilonRI receptors, leading to the in…
Ying Zhang, Ines Thiele, Dana Weekes, Zhanwen Li, Lukasz Jaroszewski, Krzysztof Ginalski, Ashley Deacon, John Wooley, Scott Lesley, Ian Wilson, Bernhard Palsson, Andrei Osterman, Adam Godzik. Three-Dimensional Structural View of the Central Metabolic Network of Thermotoga maritima. Science. 2009 Sep 18;325(5947):1544-9.. Alexey M. Eroshkin, Andrew LeBlanc, Dana Weekes, Kai Post, Zhanwen Li, Akhil Rajput, Sal T. Butera, Dennis R. Burton, Adam Godzik. bNAber: database of broadly neutralizing HIV antibodies. Nucl. Acids Res. 2013; published on November 7, 2013.. Ye Y, Godzik A. FATCAT: a web server for flexible structure comparison and structure similarity searching. Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W582-5 ...
Immediate selective hypersensitivity reactions to pyrazolones are thought to be mediated by specific-IgE antibodies. The diagnosis of selective reactions to pyrazolones is mainly based on the clinical history, skin testing and/or drug provocation test (DPT). Skin testing has low sensitivity and has potential risk of eliciting an anaphylactic response as well as DPT. Therefore the use of in vitro tests could be a safe and straightforward approach, although till now no well-validated test is available. Basophil activation test (BAT) has been proposed as a new in vitro diagnostic tool for different drug reactions although its sensibility is not optimal. The aim of this study was to analyze the potential usefulness of dipyrone metabolites to improve BAT sensitivity. ...
The clavulanic acid (CLV) is nowadays frequently included in the treatment with Amoxicillin (AX). This component of the antibiotic therapy initially thought to have a low immunogenic capacity; however immediate allergic reactions to CLV have been reported in a 30% of patients allergic to AX-CLV. Basophil activation test (BAT) has shown promising results demonstrating specific recognition of CLV determinants. The aim of this study was to assess the value of BAT in the evaluation of immediate allergic reactions to CLV. ...
Looking for basophil degranulation test? Find out information about basophil degranulation test. A white blood cell with granules that stain with basic dyes and are water-soluble Explanation of basophil degranulation test
B. Eberlein Basophil activation test in the diagnosis of insect venom allergies Clinical & Experimental Allergy 39. Version of Record online: 21 OCT 2009 , DOI: 10.1111/j.1365-2222.2009.03375.x. Complete the form below and we will send an e-mail message containing a link to the selected article on your behalf. Required = Required Field. ...
In the face of increasing prevalence of hypersensitivity reactions, introduction of effective, reliable and safe methods plays a crucial role in their diagnosing. Among the currently available laboratory (in vitro) methods is basophil activation test (BAT). It is a flow- cytometry based assay that allows to identificate in the blood sample basophils and additionally to asses the degree of cell activation after exposure to an antigen. The most common superficial identification markers are CD63 and CD203c, which increase in number after activation. Basophil actvation test can be applied to confirm diagnosis of allergy to Hymenoptera venoms, food, pollens and hypersensitivity to drugs. The aim of present paper is to present theoretical methods of this test as well as its pros and cons. We focus also on presentation of clinical case where BAT seemed to be a necessary addition to a routine diagnostic pathway. We present a case of identification of the culprit drug which caused an anaphylactic ...
Tetraspanins are a superfamily of glycoproteins that function as organisers of membranes by clustering with each other to form tetraspanin-enriched microdomains, into which certain other receptors and signalling proteins are recruited and regulated. Tetraspanin microdomains have been implicated in a range of biological processes including cell signalling, adhesion, intracellular trafficking, cell-cell fusion and viral entry. The tetraspanin CD37 was recently shown to negatively regulate the C-type lectin-like receptor dectin-1, which is essential for innate immune responses to fungal pathogens. The aim of this thesis was to firstly develop a cell line model system to investigate the mechanism by which tetraspanins inhibit dectin-1, and to secondly extend this work to the dectin-1-related CLEC-2, which is essential for platelet thrombus formation and stability. Using a nuclear factor of activated T-cells (NFAT) transcriptional reporter assay in the Jurkat T-cell line, transient over-expression ...
Previous article in issue: Pistachio vicilin, Pis v 3, is immunoglobulin E-reactive and cross-reacts with the homologous cashew allergen, Ana o 1 Previous article in issue: Pistachio vicilin, Pis v 3, is immunoglobulin E-reactive and cross-reacts with the homologous cashew allergen, Ana o 1 ...
The tetraspanins are a superfamily of transmembrane proteins with diverse functions and can form extended microdomains within the plasma membrane in conjunction with partner proteins, which probably includes receptors for bacterial adhesins. Neisseria meningitidis, the causative agent of meningococcal disease, attaches to host nasopharyngeal epithelial cells via type IV pili and opacity (Opa) proteins. We examined the role of tetraspanin function in Neisseria meningitidis adherence to epithelial cells. Tetraspanins CD9, CD63, and CD151 were expressed by HEC-1-B and DETROIT 562 cells. Coincubation of cells with antibodies against all three tetraspanin molecules used individually or in combination, with recombinant tetraspanin extracellular domains (EC2), or with small interfering RNAs (siRNAs) significantly reduced adherence of Neisseria meningitidis. In contrast, recombinant CD81, a different tetraspanin, had no effect on meningococcal adherence. Antitetraspanin antibodies reduced the adherence ...
Abstract Our research is aimed at understanding the mechanism of action of tetraspanins. This is a multi-gene family, which has shown remarkable conservation over evolution and whose members are expressed in mammals, insects and nematodes. Tetraspanins are also widely expressed in most cell types, forming molecular associations with different proteins in the different cell types. The tetraspanin CD81 was originally identified in our laboratory as a receptor that controls cell growth. To better define the role of CD81 we created CD81-deficient mice. These mice have impairments in their immune, nervous and reproductive systems. CD81 has been implicated in the pathogenesis of two major human diseases: hepatitis C virus (HCV) and malaria. CD81 is the putative receptor for HCV, CD81 is also required for infection by malaria. Plasmodium sporozoites mature in the liver to merozoites, the stage that infects red blood cells, this maturation step is CD81-dependent.. Recent Studies CD81 is a widely ...
U.S., June 15 -- ClinicalTrials.gov registry received information related to the study (NCT03182491) titled Mechanisms of Anaphylaxis on June 7. Brief Summary: The purpose of this study is to explore different mechanisms for anaphylaxis and find novel biomarkers for this hypersensitivity syndrome. The study participants are patients with anaphylaxis, patients with mild allergic reactions, and patients with febrile transfusion reactions. We will also include a group of healthy controls. Study Start Date: Study Type: Observational [Patient Registry] Condition: Anaphylaxis Allergy Transfusion Reaction Febrile Transfusion Reaction Intervention: Diagnostic Test: Biomarkers (platelet activating factor [PAF], anaphylatoxins) and basophil activation test (BAT) Analysis of biomarkers and basophil activation test Recruitment Status: Recruiting Sponsor: Haukeland University Hospital Information provided by (Responsible Party): Haukeland University Hospital Published by HT Digital Content Services with ...
Although tetraspanins are considered to be organizers (in cis) of cellular membranes, their overall job description remains relatively vague. One of the few specific functions that have been clearly established for several members of the tetraspanin family is their regulation (positive or negative) of membrane fusion processes. For example, one of the tetraspanins that we identified to be present at HIV-1 release sites, CD9, is absolutely required for sperm-egg fusion. Although it remains unknown how exactly tetraspanins control fusion processes, it is thought that they do so by manipulating the interactions of fusogenic proteins with other membrane proteins (integral or peripheral). Given these fusion regulation functions, and given that viral infections require the fusion of viral and cellular membranes, we reasoned that tetraspanins might also regulate virus-mediated fusion processes. We thus started to analyse whether overexpression (either transient or stable) or down-regulation of ...
Tetraspanins are family of small membrane proteins and they are involved in multitude of biological process. Structurally theyare characterized by having four transmembrane domains, short inner and outer loops, one large extra cellular loop containsCCG motif and N and C terminal. Iconic features of these proteins are formation of Tetraspanin Enriched Micro domains(TEMs) by interacting among themselves and with other transmembrane and cytosolic proteins. These domains provide asignaling platform for many important cellular functions such as immune response induction, fertilization, viral infection,maintenance of skin integrity and malignant process. Tetraspanin CD151 is frequently over expressed on cancer cells and isfunctionally linked to cancer metastasis. CD151 forms direct and stable and interaction with integrin molecules and regulatesthe cellular functions. Increasing evidence emerging from in vitro, in vivo and clinical analyses associates that CD151partnership with integrins ?6?1 and ...
Pronephric cilia in ocrl-/- zebrafish.A. Confocal images of pronephric cilia, detected using anti-acetylated tubulin antibody, in wild-type, ocrl-/- mutant, con
basophil - MedHelps basophil Center for Information, Symptoms, Resources, Treatments and Tools for basophil. Find basophil information, treatments for basophil and basophil symptoms.
Title: Investigating the molecular mechanism of COPD in tetraspanin CD9/CD81 DKO mice- a new model for ageing. 6/9 Yuko Tsuchiya. 6/16 Special seminar 15:00 ...
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Clone FR3-16A11 recognizes CD203c, a glycosylated type II transmembrane molecule that belongs to the family of ecto-nucleotide pyrophosphatase/ phosphodiesterase (E-NPP3) enzymes. Among hematopoietic cells, expression of CD203c is restricted to basophils as well as to mast cells and their precursors, and has been described as specific for this lineage. Protein and/or mRNA expression of CD203c has also been found in solid tissues such as uterus or prostate. Basophils and mast cells are key producers of mediators that drive the onset of inflammatory responses, e.g., in allergy. Allergen challenge leads to a rapid up-regulation of activation markers such as CD203c or CD63. Due to its restricted expression pattern, CD203c is discussed as a specific marker to monitor the allergen-induced activation of basophils, e.g., in flow cytometric basophil activation tests of the peripheral blood. - Great Britain
E xosomes are a discrete population of vesicles that are secreted from various cell types to the extracellular media. Characterization of exosomes from differ...
BioAssay record AID 375664 submitted by ChEMBL: Activity at CB2 receptor in human basophil assessed as reduction of anti-IgE-FITC conjugated antibody-stimulated CD203c expression after 30 mins by flow cytometric analysis in presence of 100 nM AM251.
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Human immunodeficiency virus type 1 (HIV-1) transmission takes place primarily through cell-cell contacts known as virological synapses. Formation of these transient adhesions between infected and uninfected cells can lead to transmission of viral particles followed by separation of the cells. Alternatively, the cells can fuse, thus forming a syncytium. Tetraspanins, small scaffolding proteins that are enriched in HIV-1 virions and actively recruited to viral assembly sites, have been found to negatively regulate HIV-1 Env-induced cell-cell fusion. How these transmembrane proteins inhibit membrane fusion, however, is currently not known. As a first step towards elucidating the mechanism of fusion repression by tetraspanins, e.g., CD9 and CD63, we sought to identify the stage of the fusion process during which they operate. Using a chemical epistasis approach, four fusion inhibitors were employed in tandem with CD9 overexpression. Cells overexpressing CD9 were found to be sensitized to inhibitors
Tetraspanins function as molecular organizers of multi-protein complexes by assembling primary complexes of a relatively low mass into extensive networks involved in cellular signalling. In this paper, we summarize our studies performed on the tetraspanin D6.1A/CO-029/TM4SF3 expressed by rat carcinoma cells. Primary complexes of D6.1A are almost indistinguishable from complexes isolated with anti-CD9 antibody. Indeed, both tetraspanins directly associate with each other and with a third tetraspanin, CD81. Moreover, FPRP (prostaglandin F2α receptor-regulatory protein)/EWI-F/CD9P-1), an Ig superfamily member that has been described to interact with CD9 and CD81, is also a prominent element in D6.1A complexes. Primary complexes isolated with D6.1A-specific antibody are clearly different from complexes containing the tetraspanin CD151. CD151 is found to interact only with D6.1A if milder conditions, i.e. lysis with LubrolWX instead of Brij96, are applied to disrupt cellular membranes. CD151 ...
Thomas Schneider is the author of these articles in the Journal of Visualized Experiments: Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity, Quasi-light Storage for Optical Data Packets, Studying Soft-matter and Biological Systems over a Wide Length-scale from Nanometer and Micrometer Sizes at the Small-angle Neutron Diffractometer KWS-2
UK-based Wickham Laboratories has introduced the monocyte activation test (MAT) as the latest addition to its portfolio of in vitro alternatives to in vivo testing methods.
human CD231 antigen: tetraspanin protein; has the unique potential to modulate HIV-1 infectivity through incorporation into released HIV-1 particles
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
The inhibitory myeloid immunoglobulin receptor CD300a (IRp60) has been shown to downregulate mast cell and eosinophil activities, thereby serving as a potential target for inhibiting allergic effector cell input in allergy. Our aims were to study the expression and functional properties of this receptor in purified human basophils, cells that crucially contribute to Th2-type immunity and allergy. Basophils homogeneously expressed CD300a as well as the inhibitory receptor CD200R on their cell surface, and these expressions increased after anti-IgE stimulation. IgE-mediated basophil degranulation was also significantly inhibited by crosslinking of either CD200R or CD300a (by 90% and 50%, respectively). Inhibitory SHIP-1 phosphorylations were also induced by CD200R and CD300a, although they were not noticeably increased by IgE-dependent activation. We conclude that both CD200R and CD300a play a role in reducing IgE-mediated basophil function and may crucially govern the known differential ...
Early studies pointed to the potential of tetraspanins to associate with a wide variety of partner proteins in a tetraspanin web. Not only do tetraspanins associate with each other, but they also associate with many Ig superfamily proteins, proteoglycans, complement regulatory proteins, integrins, growth factors, growth factor receptors, and signaling enzymes (for review see Boucheix and Rubinstein, 2001). Assembly of the tetraspanin web is based on multiple levels of interactions (Serru et al., 1999; Stipp et al., 2001a,b; and references therein). The first level includes primary interactions between specific tetraspanins and other proteins. These interactions are direct, and resist disruption by detergents such as Brij96, digitonin, and/or Triton X-100 (see examples below). Second level interactions are indirect, more numerous, and much more sensitive to disruption by Brij96, digitonin, and/or Triton X-100. Soluble second level complexes (maintained in detergents such as Brij56, Brij99, and ...
CD9, a member of the tetraspanin superfamily of proteins participates in the regulation of cell adhesive functions such as cell migration. The mechanisms underlying CD9 mediated cell migration are not known. In the current study, we investigated the molecular basis for the CD9 promoted cell migration. Our findings show that the phosphatidylinositol-3 kinase (PI-3K) inhibitors, wortamannin and LY294002 inhibited CD9 promoted cell motility in Chinese hamster ovary (CHO) cells. In contrast, inhibitors targeting protein kinase C or mitogen-activated protein kinase had no effect on CD9 driven CHO cell motility. Furthermore, inhibition of PI-3K activity in CHO cells by dominant/negative PI-3K cDNA transfection abolished CD9 mediated pro-migratory effects. Consistent with these observations, CD9 expression in CHO cells and in the rat aortic smooth muscle (RASM) cells induced enhanced phosphorylation of PI-3K substrate, Akt. In CHO cells, CD9 expression also enhanced protein levels and tyrosine phosphorylation
Scientists from the Institute for Biomedical Research (IRB Barcelona) have carried out successful in vitro tests using wasp venom to kill tumour cells. The next step will be to test its efficacy in mouse models.
Exosomes are nanosized vesicles released from every cell in the body including those in the respiratory tract and lungs. They are found in most body fluids and contain a number of different biomolecules including proteins, lipids, and both mRNA and noncoding RNAs. Since they can release their contents, particularly
Define basophil: a basophilic substance or structure; especially : a white blood cell containing basophilic granules that is similar in function to a…

CD63 antigen isoform X1 [Homo sapiens] - Protein - NCBICD63 antigen isoform X1 [Homo sapiens] - Protein - NCBI

CD63 antigen isoform X1 [Homo sapiens] CD63 antigen isoform X1 [Homo sapiens]. gi,1370463253,ref,XP_024305051.1, ... Altered expression of CD63 and exosomes in scleroderma dermal fibroblasts. [J Dermatol Sci. 2016] Altered expression of CD63 ... The CD63 gene is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, zebrafish, fruit fly, mosquito, C.elegans, and ...
more infohttps://www.ncbi.nlm.nih.gov/protein/XP_024305051.1

Cd63 - CD63 antigen - Mus musculus (Mouse) - Cd63 gene & proteinCd63 - CD63 antigen - Mus musculus (Mouse) - Cd63 gene & protein

sp,P41731,CD63_MOUSE CD63 antigen OS=Mus musculus OX=10090 GN=Cd63 PE=1 SV=2 ... "CD63 is an essential cofactor to leukocyte recruitment by endothelial P-selectin.". Doyle E.L., Ridger V., Ferraro F., Turmaine ... "CD63 is an essential cofactor to leukocyte recruitment by endothelial P-selectin.". Doyle E.L., Ridger V., Ferraro F., Turmaine ... "The tetraspanin CD63 is required for efficient IgE-mediated mast cell degranulation and anaphylaxis.". Kraft S., Jouvin M.H., ...
more infohttps://www.uniprot.org/uniprot/P41731

CD63 - CD63 antigen - Oryctolagus cuniculus (Rabbit) - CD63 gene & proteinCD63 - CD63 antigen - Oryctolagus cuniculus (Rabbit) - CD63 gene & protein

sp,Q28709,CD63_RABIT CD63 antigen OS=Oryctolagus cuniculus OX=9986 GN=CD63 PE=2 SV=2 ... CD63_RABIT. ,p>This subsection of the Entry information section provides one or more accession number(s). These are stable ...
more infohttps://www.uniprot.org/uniprot/Q28709

Antigens, CD63 | Profiles RNSAntigens, CD63 | Profiles RNS

CD63" by people in this website by year, and whether "Antigens, CD63" was a major or minor topic of these publications. ... "Antigens, CD63" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... Below are the most recent publications written about "Antigens, CD63" by people in Profiles. ... Below are MeSH descriptors whose meaning is more general than "Antigens, CD63". ...
more infohttps://profiles.rush.edu/display/26011

Validação do Teste de ativação de basófilos no diagnóstico de reações de hipersensibilidade...Validação do Teste de ativação de basófilos no diagnóstico de reações de hipersensibilidade...

Antigens CD63. Basophil degranulation test/methods. Basophils. Drug hypersensitivity/blood. Drug hypersensitivity/diagnosis. ... Antígenos CD63. Antiinflamatórios não esteróides. Basófilos. Curva ROC. Hipersensibilidade a drogas/diagnóstico. ... BAT consisted of incubating whole blood with NSAIDs, then triple-labeled with monoclonal antibodies (CD45, anti-IgE, CD63) for ... anti-IgE e CD63) para posterior leitura por citometria de fluxo. Os resultados obtidos foram comparados com as histórias ...
more infohttps://www.teses.usp.br/teses/disponiveis/5/5146/tde-24062013-152145/pt-br.php

Biomarkers - Olink	Biomarkers - Olink

CD63 antigen (CD63). Links to databases. *Uniprot P08962. Functions as cell surface receptor for TIMP1 and plays a role in the ... These curves are generated during the assay validation process using recombinant antigens. Please note that when analyzing ...
more infohttps://www.olink.com/products/neuro-exploratory-panel/biomarkers/?biomarkerId=1596

GO Gene ListGO Gene List

Cd63. CD63 antigen. NM_001042580. NM_007653. Gene Info. Cdh13. Cadherin 13. NM_019707. Gene Info. ... CD300A antigen. NM_170758. Gene Info. Cd36. CD36 antigen. NM_001159558. NM_007643. NM_001159555. NM_001159557. NM_001159556. ... CD47 antigen (Rh-related antigen, integrin-associated signal transducer). NM_010581. Gene Info. ...
more infohttps://cgap.nci.nih.gov/Genes/GoGeneQuery?PAGE=1&ORG=Mm&GOID=0060627

KEGG BRITE: CD Molecules - Homo sapiens (human)KEGG BRITE: CD Molecules - Homo sapiens (human)

... platelet K06497 CD63; CD63 antigen K06498 FCGR1A; high affinity immunoglobulin gamma Fc receptor I K06499 CEACAM; ... CD79A antigen K06507 CD79B; CD79B antigen K05412 CD80; CD80 antigen K06508 CD81; CD81 antigen K06509 KAI1; CD82 antigen K06510 ... CD300 antigen K06719 CD300; CD300 antigen K06719 CD300; CD300 antigen K06719 CD300; CD300 antigen K06721 CLEC10A; C-type lectin ... CD96 antigen K08446 ADGRE5; CD97 antigen K06519 SLC3A2; solute carrier family 3, member 2 K06520 CD99; CD99 antigen K06521 ...
more infohttps://www.genome.jp/kegg-bin/get_htext?hsa04090+4486

CD63 : Wikis (The Full Wiki)CD63 : Wikis (The Full Wiki)

CD63 antigen is a protein that in humans is encoded by the CD63 gene.[1] ... "CD63 antigen. A novel lysosomal membrane glycoprotein, cloned by a screening procedure for intracellular antigens in eukaryotic ... CD51 · CD52 · CD53 · CD54 · CD55 · CD56 · CD57 · CD58 · CD59 · CD61 · CD62 (E, L, P) · CD63 · CD64 (A, B, C) · CD66 (a, b, c, d ... Hotta H, Miyamoto H, Hara I, et al. (1992). "Genomic structure of the ME491/CD63 antigen gene and functional analysis of the 5 ...
more infohttp://www.thefullwiki.org/CD63

cd63, CD63 molecule - Creative Biogenecd63, CD63 molecule - Creative Biogene

CD63 antigen; tspan-30; granulophysin; tetraspanin-30; melanoma-associated antigen MLA1; CD63 antigen (melanoma 1 antigen); ... CD63; CD63 molecule; MLA1; ME491; LAMP-3; OMA81H; TSPAN30; ... melanoma-associated antigen ME491; ocular melanoma-associated ... antigen; lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3; cd163; zgc:65825; zgc:77709; wu: ...
more infohttps://www.creative-biogene.com/symbolsearch_cd63.html

KAKEN - Research Projects | Study for gene transfection and intracellular distribution of transfected DNA. (KAKENHI-PROJECT...KAKEN - Research Projects | Study for gene transfection and intracellular distribution of transfected DNA. (KAKENHI-PROJECT...

Publications] T. Furuno: Surface expression of CD63 antigen (AD1 antigen) in P815 cells by transfected IgE receptors. Biochem ... Publications] T.Furuno: Surface expression of CD63 antigen (AD1 antigen) in P815 mastocytoma cells by transfected IgE ... Publications] T.Furuno: Surface expression of CD63 antigen (AD1 antigen) in P815 mastocytoma cells by transfected IgE ... Publications] H Katayama: Triggering of calcium signals in antigen-specific B cells on the supported lipid monolayes Biochem. ...
more infohttps://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07558099/

CD63-GFP traffics from secretory to endocytic compartme | Open-iCD63-GFP traffics from secretory to endocytic compartme | Open-i

A-G) An 8-h pulse (at 28.5°C) of CD63-GFP expression was chased at 18°C for 0-60 h in ... CD63-GFP traffics from secretory to endocytic compartments in SCs. ( ... fig3: CD63-GFP traffics from secretory to endocytic compartments in SCs. (A-G) An 8-h pulse (at 28.5°C) of CD63-GFP expression ... fig3: CD63-GFP traffics from secretory to endocytic compartments in SCs. (A-G) An 8-h pulse (at 28.5°C) of CD63-GFP expression ...
more infohttps://openi.nlm.nih.gov/detailedresult.php?img=PMC4151142_JCB_201401072_Fig3&req=4

CD63 - Beckman CoulterCD63 - Beckman Coulter

The CD63 antigen, also known as lysosomal membrane associated glycoprotein 3 (LAMP3), is a member of the tetraspanin (TM4SF) ... CD63 Antigen. The CD63 antigen, also known as lysosomal membrane associated glycoprotein 3 (LAMP3), is a member of the ... CD63 has been found to be identical to the ME491 antigen expressed by melanoma cells. It is also found on monocytes / ... CD63 antigen, present in azurophilic granules of non-stimulated neutrophils, is strongly expressed on the surface of ...
more infohttps://www.mybeckman.pl/reagents/coulter-flow-cytometry/antibodies-and-kits/single-color-antibodies/cd63

CD63 - Beckman CoulterCD63 - Beckman Coulter

The CD63 antigen, also known as lysosomal membrane associated glycoprotein 3 (LAMP3), is a member of the tetraspanin (TM4SF) ... CD63 Antigen. The CD63 antigen, also known as lysosomal membrane associated glycoprotein 3 (LAMP3), is a member of the ... CD63 has been found to be identical to the ME491 antigen expressed by melanoma cells. It is also found on monocytes / ... CD63 antigen, present in azurophilic granules of non-stimulated neutrophils, is strongly expressed on the surface of ...
more infohttps://www.beckman.com.au/reagents/coulter-flow-cytometry/antibodies-and-kits/single-color-antibodies/cd63

CD63 - Beckman CoulterCD63 - Beckman Coulter

The CD63 antigen, also known as lysosomal membrane associated glycoprotein 3 (LAMP3), is a member of the tetraspanin (TM4SF) ... CD63 Antigen. The CD63 antigen, also known as lysosomal membrane associated glycoprotein 3 (LAMP3), is a member of the ... CD63 has been found to be identical to the ME491 antigen expressed by melanoma cells. It is also found on monocytes / ... CD63 antigen, present in azurophilic granules of non-stimulated neutrophils, is strongly expressed on the surface of ...
more infohttps://www.mybeckman.ca/reagents/coulter-flow-cytometry/antibodies-and-kits/single-color-antibodies/cd63

Code System ConceptCode System Concept

Lymphocyte antigen CD63 Current Synonym true false 1232247016 CD63 - Cluster of differentiation antigen 63 Current Synonym true ... Lymphocyte antigen CD63 (substance). Code System Preferred Concept Name. Lymphocyte antigen CD63 (substance). ...
more infohttps://phinvads.cdc.gov/vads/ViewCodeSystemConcept.action?oid=2.16.840.1.113883.6.96&code=6178009

CD63 antibodies, mouse - Primary antibodies - Antibodies - MACS Flow Cytometry - Products - Miltenyi BiotecCD63 antibodies, mouse - Primary antibodies - Antibodies - MACS Flow Cytometry - Products - Miltenyi Biotec

Interaction of CD63 with other membrane proteins or adaptors regulates cell activities such as adhesion, migration, and ... CD63 is expressed on monocytes, macrophages, endothelium, fibroblasts, osteoclasts, smooth muscle cells, and activated ... Clone REA563 recogizes the mouse CD63 antigen. It belongs to the tetraspanin transmembrane 4 superfamily (TM4SF) of proteins, ... Clone REA563 recogizes the mouse CD63 antigen. It belongs to the tetraspanin transmembrane 4 superfamily (TM4SF) of proteins, ...
more infohttps://www.miltenyibiotec.com/BE-en/products/macs-flow-cytometry/antibodies/primary-antibodies/cd63-antibodies-mouse-rea563-1-10.html

The effect of a leukodepletion model on the activation stage of platelets | SpringerLinkThe effect of a leukodepletion model on the activation stage of platelets | SpringerLink

CD 63 antigen. A novel lysosomal membrane glycoprotein, cloned by a screening procedure for intracellular antigens in in ...
more infohttps://link.springer.com/article/10.2478/s11536-010-0062-1

Similar articles for PubMed (Select 11411598) - PubMed - NCBISimilar articles for PubMed (Select 11411598) - PubMed - NCBI

Detection of allergen-induced basophil activation by expression of CD63 antigen using a tricolour flow cytometric method. ... For allergy diagnostic flow cytometry, detection of CD203c instead of CD63 is not at all an improvement in other hands. ... Comments regarding Marked improvement of the basophil activation test by detecting CD203c instead of CD63 by Boumiza et al. ... A new variant of the basophil activation test for allergen-induced basophil CD63 upregulation. The effect of cetirizine. ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed?LinkName=pubmed_pubmed&from_uid=11411598

CD63 - WikipediaCD63 - Wikipedia

CD63 antigen is a protein that in humans is encoded by the CD63 gene. CD63 is mainly associated with membranes of intracellular ... 1991). "CD63 antigen. A novel lysosomal membrane glycoprotein, cloned by a screening procedure for intracellular antigens in ... In cell biology, CD63 is often used as a marker for multivessicular bodies, which are enriched with CD63. CD63 has been shown ... CD63 protein, human at the US National Library of Medicine Medical Subject Headings (MeSH) Human CD63 genome location and CD63 ...
more infohttps://en.wikipedia.org/wiki/CD63

Basolateral sorting and transcytosis define the Cu+-regulated translocation of ATP7B to the bile canaliculus | Journal of Cell...Basolateral sorting and transcytosis define the Cu+-regulated translocation of ATP7B to the bile canaliculus | Journal of Cell...

Expression of GMP-140 and LIMP-CD63 (CD63 antigen) in human lymphoid tissues. Virchows Arch. B Cell Pathol. Incl. Mol. Pathol. ... 3A) (Barriocanal et al., 1986; Metzelaar et al., 1992). Two of these proteins, CD63 and LAMP1, have been used in the study that ... 6C1-F3). Separate study of the association of CD63, LIMPII and LAMP1 with the ATP7B transiting through the SAC in cells that ... Moreover, in three separate studies of the association of CD63, LIMPII and LAMP1 with the bile canaliculus of cells that had ...
more infohttp://jcs.biologists.org/content/129/11/2190

CD63 Antibody (LAMP3/968) [Janelia Fluor® 549] (NBP2-47936JF549): Novus BiologicalsCD63 Antibody (LAMP3/968) [Janelia Fluor® 549] (NBP2-47936JF549): Novus Biologicals

Mouse Monoclonal Anti-CD63 Antibody (LAMP3/968) [Janelia Fluor® 549]. Late Endosomes Marker. Validated: Flow, IHC, IHC-P, ICC/ ... Alternate Names for CD63 Antibody (LAMP3/968) [Janelia Fluor® 549]. *CD63 antigen (melanoma 1 antigen) ... Blogs on CD63. Check out the latest blog posts on CD63.. Tools for Isolation, Quantification and Analysis of Exosomes. Exosomes ... CD63: is it pro-metastatic or anti-metastatic?. CD63 is a type II membrane protein belonging to tetraspanin superfamily and it ...
more infohttps://www.novusbio.com/products/cd63-antibody-lamp3-968_nbp2-47936jf549

CD63 Antibody (MX-49.129.5) [DyLight 488] (NBP2-34689G): Novus BiologicalsCD63 Antibody (MX-49.129.5) [DyLight 488] (NBP2-34689G): Novus Biologicals

Mouse Monoclonal Anti-CD63 Antibody (MX-49.129.5) [DyLight 488]. Late Endosomes Marker. Validated: WB, ELISA, Flow, ICC/IF, IHC ... Alternate Names for CD63 Antibody (MX-49.129.5) [DyLight 488]. *CD63 antigen (melanoma 1 antigen) ... Blogs on CD63. Check out the latest blog posts on CD63.. Tools for Isolation, Quantification and Analysis of Exosomes. Exosomes ... Reviews for CD63 Antibody (NBP2-34689G) (0) There are no reviews for CD63 Antibody (NBP2-34689G). By submitting a review you ...
more infohttps://www.novusbio.com/products/cd63-antibody-mx-491295_nbp2-34689g

IMP: Integrative Multi-species PredictionIMP: Integrative Multi-species Prediction

cd63. Cd63 antigen. 0.581. med12. mediator of RNA polymerase II transcription, subunit 12 homolog. 0.564. ...
more infohttp://imp.princeton.edu/predictions/process_ortho/zebrafish-context-global/15403/?gene=49809

CD63 antibodies, mouse - Primary antibodies - Antibodies - MACS Flow Cytometry - Products - Miltenyi Biotec - USACD63 antibodies, mouse - Primary antibodies - Antibodies - MACS Flow Cytometry - Products - Miltenyi Biotec - USA

Interaction of CD63 with other membrane proteins or adaptors regulates cell activities such as adhesion, migration, and ... CD63 is expressed on monocytes, macrophages, endothelium, fibroblasts, osteoclasts, smooth muscle cells, and activated ... Clone REA563 recogizes the mouse CD63 antigen. It belongs to the tetraspanin transmembrane 4 superfamily (TM4SF) of proteins, ... Clone REA563 recogizes the mouse CD63 antigen. It belongs to the tetraspanin transmembrane 4 superfamily (TM4SF) of proteins, ...
more infohttps://www.miltenyibiotec.com/US-en/products/macs-flow-cytometry/antibodies/primary-antibodies/cd63-antibodies-mouse-rea563-1-10.html?countryRedirected=1
  • The CD63 antibodies inhibit adhesion of neutrophils to activated endothelium. (mybeckman.pl)
  • As the emitted fluorescence intensity is proportional to the binding sites of each single cell, the intensity will increase according to the number of FITC- conjugated antibodies bound to CD63 expressing cells. (wikipedia.org)
  • CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. (novusbio.com)
  • CD63, as other tetraspanins (CD9, CD81, CD82), has recently been reported as forming complexes with VLA-3 and phosphatidylinositol 4-kinase, with VLA-6, CD11/CD18 and tyrosine kinase. (mybeckman.pl)
  • CD63 is mainly associated with membranes of intracellular vesicles, although cell surface expression may be induced. (wikipedia.org)
  • CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. (novusbio.com)
  • CLBGran/12 was used as a CD63 reference monoclonal antibody during HLDA 6. (mybeckman.pl)
  • There are currently no images for CD63 Antibody (NBP2-47936JF549). (novusbio.com)
  • Altered expression of CD63 and exosomes in scleroderma dermal fibroblasts. (nih.gov)
  • Novel structurally distinct family of leucocyte surface glycoproteins including CD9, CD37, CD53 and CD63. (thefullwiki.org)
  • CD63 was first described in granules of resting platelets and on the surface membrane on activated platelets. (mybeckman.pl)
  • CD63 antigen, present in azurophilic granules of non-stimulated neutrophils, is strongly expressed on the surface of neutrophils after activation. (mybeckman.pl)
  • Degranulated cell expose CD63 molecules on their outer cell membrane, hence the granules, which contain CD63 molecules on their inner surface, merged with the cell membrane. (wikipedia.org)
  • In the hymenoptera venom allergy, BAT is an effective tool in identifying primary sensitizing antigen and in the follow up of patient in venom immunotherapy. (elsevier.com)
  • CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression. (novusbio.com)
  • CD63 has been shown to interact with CD117 [ 2 ] and CD82 . (thefullwiki.org)
  • GFP positive in C and E) and mMVBLs (asterisks in B, D, and F in more basal views) that were CD63-GFP-positive were scored (G). Data shown are from a single representative experiment out of two repeats. (nih.gov)