An abundant lysosomal-associated membrane protein that has been found to shuttle between LYSOSOMES; ENDOSOMES; and the PLASMA MEMBRANE. Loss of expression of lysosomal-associated membrane protein 2 is associated with GLYCOGEN STORAGE DISEASE TYPE IIB.
Ubiquitously expressed integral membrane glycoproteins found in the LYSOSOME.
A lysosomal-associated membrane glycoprotein that is expressed at high levels in mature DENDRITIC CELLS.
An abundant lysosomal-associated membrane protein that has been found to shuttle between LYSOSOMES; ENDOSOMES; and the PLASMA MEMBRANE. In PLATELETS and T-LYMPHOCYTES it may play a role in the cellular degranulation process.
Cellular antigens that are specific for MELANOMA cells.
A large superfamily of cell surface membrane proteins characterized by their four transmembrane domains. They play a role in a variety of processes such as cellular adhesion and motility. They may be involved in the organization of cell surface MEMBRANE MICRODOMAINS that regulate the activation of LEUKOCYTES.
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
A plant family of the order THEALES, subclass Dilleniidae, class Magnoliopsida, best known for CAMELLIA SINENSIS, which is the source of Oriental TEA.
Antibodies produced by a single clone of cells.
A subtype of tetraspanin proteins that play a role in cell adhesion, cell motility, and tumor metastasis. CD9 antigens take part in the process of platelet activation and aggregation, the formation of paranodal junctions in neuronal tissue, and the fusion of sperm with egg.
Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
Transfer of HEMATOPOIETIC STEM CELLS from BONE MARROW or BLOOD between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS). Hematopoietic stem cell transplantation has been used as an alternative to BONE MARROW TRANSPLANTATION in the treatment of a variety of neoplasms.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Transplantation between individuals of the same species. Usually refers to genetically disparate individuals in contradistinction to isogeneic transplantation for genetically identical individuals.
A constitutively expressed subfamily of the HSP70 heat-shock proteins. They preferentially bind and release hydrophobic peptides by an ATP-dependent process and are involved in post-translational PROTEIN TRANSLOCATION.
The clinical entity characterized by anorexia, diarrhea, loss of hair, leukopenia, thrombocytopenia, growth retardation, and eventual death brought about by the GRAFT VS HOST REACTION.
Preparative treatment of transplant recipient with various conditioning regimens including radiation, immune sera, chemotherapy, and/or immunosuppressive agents, prior to transplantation. Transplantation conditioning is very common before bone marrow transplantation.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Tumors or cancer of the MOUTH.
A carcinoma derived from stratified SQUAMOUS EPITHELIAL CELLS. It may also occur in sites where glandular or columnar epithelium is normally present. (From Stedman, 25th ed)
A type of extracellular vesicle, containing RNA and proteins, that is secreted into the extracellular space by EXOCYTOSIS when MULTIVESICULAR BODIES fuse with the PLASMA MEMBRANE.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
A cell line derived from cultured tumor cells.
The process of altering the morphology and functional activity of macrophages so that they become avidly phagocytic. It is initiated by lymphokines, such as the macrophage activation factor (MAF) and the macrophage migration-inhibitory factor (MMIF), immune complexes, C3b, and various peptides, polysaccharides, and immunologic adjuvants.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.

Altered trafficking of lysosomal proteins in Hermansky-Pudlak syndrome due to mutations in the beta 3A subunit of the AP-3 adaptor. (1/236)

Hermansky-Pudlak syndrome (HPS) is a genetic disorder characterized by defective lysosome-related organelles. Here, we report the identification of two HPS patients with mutations in the beta 3A subunit of the heterotetrameric AP-3 complex. The patients' fibroblasts exhibit drastically reduced levels of AP-3 due to enhanced degradation of mutant beta 3A. The AP-3 deficiency results in increased surface expression of the lysosomal membrane proteins CD63, lamp-1, and lamp-2, but not of nonlysosomal proteins. These differential effects are consistent with the preferential interaction of the AP-3 mu 3A subunit with tyrosine-based signals involved in lysosomal targeting. Our results suggest that AP-3 functions in protein sorting to lysosomes and provide an example of a human disease in which altered trafficking of integral membrane proteins is due to mutations in a component of the sorting machinery.  (+info)

PETA-3/CD151, a member of the transmembrane 4 superfamily, is localised to the plasma membrane and endocytic system of endothelial cells, associates with multiple integrins and modulates cell function. (2/236)

The Transmembrane 4 Superfamily member, PETA-3/CD151, is ubiquitously expressed by endothelial cells in vivo. In cultured human umbilical vein endothelial cells PETA-3 is present on the plasma membrane and predominantly localises to regions of cell-cell contact. Additionally, this protein is abundant within an intracellular compartment which accounts for up to 66% of the total PETA-3 expressed. Intracellular PETA-3 showed colocalisation with transferrin receptor and CD63 suggesting an endosomal/lysosomal localisation which was supported by immuno-electronmicroscopy studies. Co-immunoprecipitation experiments investigating possible interactions of PETA-3 with other molecules demonstrated associations with several integrin chains including beta1, beta3, beta4, (alpha)2, (alpha)3, (alpha)5, (alpha)6 and provide the first report of Transmembrane 4 Superfamily association with the (alpha)6beta4 integrin. Using 2-colour confocal microscopy, we demonstrated similar localisation of PETA-3 and integrin chains within cytoplasmic vesicles and endothelial cell junctions. In order to assess the functional implications of PETA-3/integrin associations, the effect of anti-PETA-3 antibodies on endothelial function was examined. Anti-PETA-3 mAb inhibited endothelial cell migration and modulated in vitro angiogenesis, but had no detectable effect on neutrophil transendothelial migration. The broad range of integrin associations and the presence of PETA-3 with integrins both on the plasma membrane and within intracellular vesicles, suggests a primary role for PETA-3 in regulating integrin trafficking and/or function.  (+info)

Role of autocrine stimulation on the effects of cyclic AMP on protein and lipid phosphorylation in collagen-activated and thrombin-activated platelets. (3/236)

We compared several responses in thrombin-stimulated and collagen (type I)-stimulated platelets with and without forskolin and inhibitors of autocrine stimulation (IAS: an ADP-removing system of creatine phosphate/creatine phosphokinase, Arg-Gly-Asp-Ser peptide to prevent fibrinogen/fibronectin binding to GPIIb/IIIa, SQ 29.548 as a thromboxane A2 receptor antagonist, cyproheptadine as a serotonin receptor antagonist, BN 52021 as a platelet-activating factor receptor antagonist). The pattern of tyrosine-phosphorylated proteins, the phosphorylation of lipids in the polyphosphoinositide cycle and phosphorylation of pleckstrin (P47) were studied as markers for signal-transducing responses, exposure of CD62 (P-selectin) and CD63 (Glycoprotein 53), as well as secretion of ADP + ATP and beta-N-acetyl-glycosaminidase were studied as final activation responses. Clear differences between thrombin-stimulated and collagen-stimulated platelets were observed. First, practically all protein-tyrosine phosphorylation induced by thrombin was inhibited by IAS, while a partial inhibition was observed for collagen; the phosphorylation due to collagen alone was apparently stimulated by elevation of cAMP. Secondly, the other responses to thrombin were inhibited by increased levels of cAMP, independent of autocrine stimulation. In contrast, only the autocrine part of the collagen-induced responses was inhibited by elevation of cAMP. Thus, the inhibition by elevated cAMP seen in collagen-stimulated platelets seems to be due to removal of the G-protein-mediated activation from secreted autocrine stimulators either by IAS or forskolin. The remaining activity is a pure collagen effect which is not affected by elevated levels of cAMP.  (+info)

Detection of allergen-induced basophil activation by expression of CD63 antigen using a tricolour flow cytometric method. (4/236)

In the field of allergy diagnosis, most in vitro functional tests are focused on basophils. Nevertheless, the very small number of circulating basophils limits these experiments and their clinical benefit remains controversial. As flow cytometry is a valuable tool for identifying cell populations, even at low concentrations, we developed a tricolour flow cytometric method for the study of allergen-induced basophil activation. Identification of cells was based both on CD45 expression and on the presence of IgE on the cell surface, since basophils express high-affinity receptors for IgE (Fc epsilon RI). Cell activation upon allergen challenge was assessed by the expression of CD63 antigen on the plasma membrane. Basophil isolation and activation (with the chemotactic peptide formyl-methionyl-leucyl-phenylalanine) were validated in 32 non-allergic patients. In 12 allergic patients, basophil stimulation by a relevant allergen was in most cases positive (10/12). Furthermore a concentration-dependent hook effect was observed. Of the allergic and non-allergic patients, none showed non-specific activation with an irrelevant allergen (specificity 100%). Overall, our preliminary results, even in a small population, suggest that this is a reliable and valuable method for the diagnosis of allergies complementing specific allergen IgE and skin test results. Obviously, additional clinical studies are needed to validate these first results.  (+info)

Rapid down-regulation of CD63 transcription by progesterone in human endometrial stromal cells. (5/236)

Differentiation of endometrial stromal cells (decidualization) plays a crucial role in embryo implantation and maintenance of pregnancy. While progesterone is a key factor in regulating endometrial cell decidualization, the molecular mechanisms remain unclear. In the present study, we investigated the effect of gene transcription in human endometrial stromal cells (ESC) by progesterone, oestrogen or vehicle using the polymerase chain reaction-based differential display methodology. A transcript which is down-regulated by progesterone, but not by vehicle and oestrogen, was identified from a differential display band and the progesterone sensitivity of its expression was verified in Northern blot analysis. The level of the gene expression in progesterone-treated ESC was approximately 60% of that in the vehicle- and oestrogen-treated ESC. This cDNA was revealed to be human CD63 antigen, a recently identified member of the transmembrane 4 superfamily. The inhibitory effect of progesterone is observed within 30 min after hormone treatment. In human endometrium, CD63 mRNA levels were significantly decreased (P < 0.05) during the secretory phase compared with levels during the proliferative phase. This down-regulation of CD63 in vivo elevated levels of progesterone in the secretory phase. These results suggest that CD63 transcription is down-regulated by progesterone in human endometrium.  (+info)

Monocyte activation in patients with Wegener's granulomatosis. (6/236)

OBJECTIVE: Wegener's granulomatosis (WG) is an inflammatory disorder characterised by granulomatous inflammation, vasculitis, and necrotising vasculitis and is strongly associated with anti-neutrophil cytoplasmic antibodies (ANCA). Activated monocytes/macrophages are present in renal biopsy specimens and participate in granuloma formation by synthesising and secreting a variety of chemoattractants, growth factors, and cytokines. In view of these findings, in vivo monocyte activation was evaluated in patients with WG and the findings related to parameters of clinical disease activity. METHODS: Monocyte activation was analysed by measuring plasma concentrations of soluble products of monocyte activation, that is neopterin and interleukin 6 (IL6), by ELISA, and by quantitating the surface expression of activation markers on circulating monocytes by flow cytometry. RESULTS: Twenty-four patients with active WG were included in this study. Ten of these patients were also analysed at the time of remission. Twelve patients with sepsis served as positive controls, and 10 healthy volunteers as negative controls for monocyte activation. Patients with active disease had increased monocyte activation compared with healthy controls as shown by increased concentrations of neopterin (p < 0.0001) and increased surface expression of CD11b (p < 0.05) and CD64 (p < 0.05). In those patients with increased concentrations of IL6 during active disease plasma concentrations of IL6 decreased during follow up when patients went into remission (p < 0.0001). In addition, neopterin (r = 0.37, r = 0.44), IL6 (r = 0.37, r = 0.60) and CD63 expression (r = 0.39, r = 0.45) correlated significantly with disease activity as measured by the Birmingham Vasculitis Activity Score and C reactive protein values, respectively. Compared with patients with sepsis, all markers of monocyte activation in patients with vasculitis were lower. CONCLUSION: It is concluded that disease activity in WG correlates with the extent of activation of monocytes, compatible with their role in the pathophysiology of this disease.  (+info)

Y receptor-mediated induction of CD63 transcripts, a tetraspanin determined to be necessary for differentiation of the intestinal epithelial cell line, hBRIE 380i cells. (7/236)

Peptide YY (PYY) and neuropeptide Y (NPY) are peptides that coordinate intestinal activities in response to luminal and neuronal signals. In this study, using the rat hybrid small intestinal epithelial cell line, hBRIE 380i cells, we demonstrated that PYY- and NPY-induced rearrangement of actin filaments may be in part through a Y1alpha and/or a nonneuronal Y2 receptor, which were cloned from both the intestinal mucosa and the hBRIE 380i cells. A number of PYY/NPY-responsive genes were also identified by subtractive hybridization of the hBRIE 380i cells in the presence or absence of a 6-h treatment with PYY. Several of these genes coded for proteins associated with the cell cytoskeleton or extracellular matrix. One of these proteins was the transmembrane-4 superfamily protein CD63, previously shown to associate with beta(1)-integrin and implicated in cell adhesion. CD63 immunoreactivity, using antibody to the extracellular domain, was highest in the differentiated cell clusters of the hBRIE 380i cells. The hBRIE 380i cells transfected with antisense CD63 cDNA lost these differentiated clusters. These studies suggest a new role for NPY and PYY in modulating differentiation through cytoskeletal associated proteins.  (+info)

CD63 associates with CD11/CD18 in large detergent-resistant complexes after translocation to the cell surface in human neutrophils. (8/236)

CD63 antibody binding to the neutrophil surface triggers a transient activation signal that regulates the adhesive activity and surface expression of CD11/CD18. Gel permeation chromatography demonstrated that all of the cell surface CD11/CD18 associated with CD63 eluted in the void volume, indicating that they were present in large detergent-resistant complexes. In contrast, the majority of the total cellular CD63, CD11 and CD18, which are largely intracellular, was not present in complexes. The data suggest that intracellular CD11, CD18 and CD63 are not in detergent-resistant complexes, but enter such complexes following translocation to the cell surface.  (+info)

Two lines of evidence in our current study suggest that surface TEMs can serve as exit gateways for HIV-1. First, most cell surface punctae where either Gag or Env clusters in both HeLa cell and in Jurkat T lymphocytes are also occupied by one of the tetraspanins (Figs. 6-8⇑⇑ and 10). Second, cellular TSG101 and VPS28, the components of the cellular budding machinery responsible for viral egress (Morita and Sundquist, 2004), when recruited to the plasma membrane in cells producing HIV-1, accumulate at CD63-containing TEMs (Fig. 8). Furthermore, we find that distortion of TEM distribution in virus-producing cells by an anti-CD9 antibody (K41) correlates with inhibition of HIV-1 release (unpublished data).. In a study where we used the FlAsH technique for successive dual-color labeling (Gaietta et al., 2002) of Gag in various virus-secreting cell types, we observed localization of newly synthesized Gag at distinct areas on the plasma membrane (Rudner et al., 2005). We also documented that ...
RESUMEN. The basophil activation test (BAT) has become a pervasive test for allergic response through the development of flow cytometry, discovery of activation markers such as CD63 and unique markers identifying basophil granulocytes.. Basophil activation test measures basophil response to allergen cross-linking IgE on between 150 and 2000 basophil granulocytes in ,0.1 ml fresh blood. Dichotomous activation is assessed as the fraction of reacting basophils.. In addition to clinical history, skin prick test, and specific IgE determination, BAT can be a part of the diagnostic evaluation of patients with food-, insect venom-, and drug allergy and chronic urticaria. It may be helpful in determining the clinically relevant allergen.. Basophil sensitivity may be used to monitor patients on allergen immunotherapy, anti-IgE treatment or in the natural resolution of allergy. Basophil activation test may use fewer resources and be more reproducible than challenge testing. As it is less stressful for the ...
The Republican Research and Practical Center for Epidemiology and Microbiology, Minsk, Belarus. The Basophil Activation Test (BAT) is a modern cellular method of allergy diagnosis, which due to its high efficiency has firmly taken its place in the diagnostic algorithm of food, pollen and drug allergy, and chronic urticaria. A promising direction of the BAT is to evaluate the effectiveness of specific immunotherapy, treatment with anti-IgE drugs, and the natural resolution of allergy. The current review presents the main approach of the BAT procedure and the efficacy of the use for the diagnosis for various types of allergies, as well as the rules for evaluating the diagnostic results. ...
Basophil activation in unfractionated samples such as whole blood can be measured by changes in the expression of cell surface markers or even intracellular events (e.g. phosphorylation, oxidative burst, calcium flux) by flow cytometry. Separate markers (e.g. as CD123+ HLA-DR-) are used to specifically identify basophils in addition to those used for assessment of activation. A feature of this protocol is to stimulate the basophils both in the presence and absence of autologous plasma. This protocol is specifically for a mouse allergen SCIT study conducted at JHU. ...
Tetraspanins are exposed at the surface of cellular membranes, which allows for the fixation of cognate antibodies. Developing specific antibodies in conjunction with genetic data would largely contribute to deciphering their biological behavior. In this short review, we summarize the main functions …
A change in morphology and behavior of a basophil resulting from exposure to a cytokine, chemokine, soluble factor, or to (at least in mammals) an antigen which the basophil has specifically bound via IgE bound to Fc-epsilonRI receptors, leading to the in…
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Ying Zhang, Ines Thiele, Dana Weekes, Zhanwen Li, Lukasz Jaroszewski, Krzysztof Ginalski, Ashley Deacon, John Wooley, Scott Lesley, Ian Wilson, Bernhard Palsson, Andrei Osterman, Adam Godzik. Three-Dimensional Structural View of the Central Metabolic Network of Thermotoga maritima. Science. 2009 Sep 18;325(5947):1544-9.. Alexey M. Eroshkin, Andrew LeBlanc, Dana Weekes, Kai Post, Zhanwen Li, Akhil Rajput, Sal T. Butera, Dennis R. Burton, Adam Godzik. bNAber: database of broadly neutralizing HIV antibodies. Nucl. Acids Res. 2013; published on November 7, 2013.. Ye Y, Godzik A. FATCAT: a web server for flexible structure comparison and structure similarity searching. Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W582-5 ...
The basophil activation test (BAT), in which translocation of markers to the surface of blood basophils is measured in response to allergen by flow cytometry, is a rapid assay that is gaining popularity. Two markers are currently being evaluated for the BAT; CD63 and the lineage-specific CD203c. In a recent report, detection of CD203c after lysis with Saponin was shown to be superior to detection of CD63 after lysis with formic acid. We wanted to compare a) lysis with formic acid and lysis with Saponin, b) the response through CD203c and CD63, and c) the definition 10% activated cells above background with the probability binning metric T(χ) | 4, on sets of data generated with blood basophils stimulated with varying concentrations of anti-FcεRI antibody. Blood from volunteers was incubated with serial logarithmic dilutions of anti-FcεRI and subsequently with antibodies to CD203c PE and CD63 FITC. Sets of samples set up in parallel were lysed with either Saponin based Whole Blood Lysing reagent or
This is a case report of anaphylaxis in which the basophil activation test (BAT) was used to identify the etiological agent. Although skin tests are considered the most effective methods for identifying anaphylactic triggers, the test itself presents a risk of inducing anaphylaxis. The BAT is advantageous because of its inherent lack of risk, high sensitivity and specificity to identify the suspected anaphylactic agents, and diagnostic accuracy comparable to conventional skin testing. Therefore, in the future, the BAT is likely to become the preferred test for the detection of allergens over conventional skin tests. ...
This is a case report of anaphylaxis in which the basophil activation test (BAT) was used to identify the etiological agent. Although skin tests are considered the most effective methods for identifying anaphylactic triggers, the test itself presents a risk of inducing anaphylaxis. The BAT is advantageous because of its inherent lack of risk, high sensitivity and specificity to identify the suspected anaphylactic agents, and diagnostic accuracy comparable to conventional skin testing. Therefore, in the future, the BAT is likely to become the preferred test for the detection of allergens over conventional skin tests. ...
Immediate selective hypersensitivity reactions to pyrazolones are thought to be mediated by specific-IgE antibodies. The diagnosis of selective reactions to pyrazolones is mainly based on the clinical history, skin testing and/or drug provocation test (DPT). Skin testing has low sensitivity and has potential risk of eliciting an anaphylactic response as well as DPT. Therefore the use of in vitro tests could be a safe and straightforward approach, although till now no well-validated test is available. Basophil activation test (BAT) has been proposed as a new in vitro diagnostic tool for different drug reactions although its sensibility is not optimal. The aim of this study was to analyze the potential usefulness of dipyrone metabolites to improve BAT sensitivity. ...
The clavulanic acid (CLV) is nowadays frequently included in the treatment with Amoxicillin (AX). This component of the antibiotic therapy initially thought to have a low immunogenic capacity; however immediate allergic reactions to CLV have been reported in a 30% of patients allergic to AX-CLV. Basophil activation test (BAT) has shown promising results demonstrating specific recognition of CLV determinants. The aim of this study was to assess the value of BAT in the evaluation of immediate allergic reactions to CLV. ...
TY - JOUR. T1 - Anaphylaxis to xylitol diagnosed by skin prick test and basophil activation test. AU - Okamoto, Kaoru. AU - Kagami, Michiko. AU - Kawai, Manabu. AU - Mori, Yuji. AU - Yamawaki, Kazuo. AU - Nakajima, Yoichi. AU - Kondo, Yasuto. AU - Tsuge, Ikuya. PY - 2019/1. Y1 - 2019/1. UR - http://www.scopus.com/inward/record.url?scp=85058961080&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=85058961080&partnerID=8YFLogxK. U2 - 10.1016/j.alit.2018.08.001. DO - 10.1016/j.alit.2018.08.001. M3 - Letter. C2 - 30190098. AN - SCOPUS:85058961080. VL - 68. SP - 130. EP - 131. JO - Allergology International. JF - Allergology International. SN - 1323-8930. IS - 1. ER - ...
Looking for basophil degranulation test? Find out information about basophil degranulation test. A white blood cell with granules that stain with basic dyes and are water-soluble Explanation of basophil degranulation test
B. Eberlein Basophil activation test in the diagnosis of insect venom allergies Clinical & Experimental Allergy 39. Version of Record online: 21 OCT 2009 , DOI: 10.1111/j.1365-2222.2009.03375.x. Complete the form below and we will send an e-mail message containing a link to the selected article on your behalf. Required = Required Field. ...
Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Essential for trafficking and compartmentalization of CD19 receptor on the cell surface of activated B cells (PubMed:23499492). Upon initial encounter with a microbial pathogen, enables the assembly of CD19-CR2 and B cell receptor complexes at signaling TERMs, lowering the threshold dose of antigen required to trigger B cell clonal expansion and humoral immune response (By similarity). In T cells, associates with CD4 or CD8 coreceptors and defines the maturation state of antigen-induced synapses with B cells (By similarity). Facilitates localization of CD3 in these immune synapses, required for costimulation and sustained activation of T cells, preferentially triggering T helper type 2 immune response (PubMed:11046035). Can act both as positive and negative regulator of homotypic or heterotypic cell-cell fusion processes. In myoblasts
Speakers Speakers from Authorities: Dr Klaus Cussler, Paul-Ehrlich-Institut, Germany Dr Don Singer, USP, USA Dr Ingo Spreitzer, PEI, Germany Dr Karin Nordgren, NIBSC Speakers from …
In the face of increasing prevalence of hypersensitivity reactions, introduction of effective, reliable and safe methods plays a crucial role in their diagnosing. Among the currently available laboratory (in vitro) methods is basophil activation test (BAT). It is a flow- cytometry based assay that allows to identificate in the blood sample basophils and additionally to asses the degree of cell activation after exposure to an antigen. The most common superficial identification markers are CD63 and CD203c, which increase in number after activation. Basophil actvation test can be applied to confirm diagnosis of allergy to Hymenoptera venoms, food, pollens and hypersensitivity to drugs. The aim of present paper is to present theoretical methods of this test as well as its pros and cons. We focus also on presentation of clinical case where BAT seemed to be a necessary addition to a routine diagnostic pathway. We present a case of identification of the culprit drug which caused an anaphylactic ...
Tetraspanins are a superfamily of glycoproteins that function as organisers of membranes by clustering with each other to form tetraspanin-enriched microdomains, into which certain other receptors and signalling proteins are recruited and regulated. Tetraspanin microdomains have been implicated in a range of biological processes including cell signalling, adhesion, intracellular trafficking, cell-cell fusion and viral entry. The tetraspanin CD37 was recently shown to negatively regulate the C-type lectin-like receptor dectin-1, which is essential for innate immune responses to fungal pathogens. The aim of this thesis was to firstly develop a cell line model system to investigate the mechanism by which tetraspanins inhibit dectin-1, and to secondly extend this work to the dectin-1-related CLEC-2, which is essential for platelet thrombus formation and stability. Using a nuclear factor of activated T-cells (NFAT) transcriptional reporter assay in the Jurkat T-cell line, transient over-expression ...
Subcutaneous Immunotherapy (SCIT) modifies the allergic response and relieves allergic symptoms. SCIT is the only and a very effective treatment for insect venom allergy. We hypothesized that basophil sensitivity, measured through the basophil activation test, would decrease during SCIT up dosing. Expression of CD203c was compared to CD63 as marker for basophil activation, using a Bland Altman plot and ROC curves. Patients (n = 18) starting subcutaneous SCIT for wasp allergy with an up dosing scheme of 7 to 11 weeks were enrolled. Heparinised blood samples were drawn at weeks 1-4, 7 and at the first maintenance visit. Basophils were stimulated at 7 log dilutions of V. vespula allergen for 15 min, and were stained with CD203c and CD63. Basophils were identified as CD203c+ leukocytes, and the proportion of CD63+ and CD203c+ cells were plotted against allergen concentration. A sigmoid curve was fitted to the points, and the allergen concentration at which half of the maximal activation was achieved, LC50,
Abstract Our research is aimed at understanding the mechanism of action of tetraspanins. This is a multi-gene family, which has shown remarkable conservation over evolution and whose members are expressed in mammals, insects and nematodes. Tetraspanins are also widely expressed in most cell types, forming molecular associations with different proteins in the different cell types. The tetraspanin CD81 was originally identified in our laboratory as a receptor that controls cell growth. To better define the role of CD81 we created CD81-deficient mice. These mice have impairments in their immune, nervous and reproductive systems. CD81 has been implicated in the pathogenesis of two major human diseases: hepatitis C virus (HCV) and malaria. CD81 is the putative receptor for HCV, CD81 is also required for infection by malaria. Plasmodium sporozoites mature in the liver to merozoites, the stage that infects red blood cells, this maturation step is CD81-dependent.. Recent Studies CD81 is a widely ...
U.S., June 15 -- ClinicalTrials.gov registry received information related to the study (NCT03182491) titled Mechanisms of Anaphylaxis on June 7. Brief Summary: The purpose of this study is to explore different mechanisms for anaphylaxis and find novel biomarkers for this hypersensitivity syndrome. The study participants are patients with anaphylaxis, patients with mild allergic reactions, and patients with febrile transfusion reactions. We will also include a group of healthy controls. Study Start Date: Study Type: Observational [Patient Registry] Condition: Anaphylaxis Allergy Transfusion Reaction Febrile Transfusion Reaction Intervention: Diagnostic Test: Biomarkers (platelet activating factor [PAF], anaphylatoxins) and basophil activation test (BAT) Analysis of biomarkers and basophil activation test Recruitment Status: Recruiting Sponsor: Haukeland University Hospital Information provided by (Responsible Party): Haukeland University Hospital Published by HT Digital Content Services with ...
Background:Tetraspanins are small transmembrane proteins, found in all higher eukaryotes, that compartmentalize cellular membranes through interactions with partner proteins. CD81 is a prototypical tetraspanin and contributes to numerous physiological and pathological processes, including acting as a critical entry receptor for hepatitis C virus (HCV). Antibody engagement of tetraspanins can induce a variety of effects, including actin cytoskeletal rearrangements, activation of MAPK-ERK signaling and cell migration. However, the epitope specificity of most anti-tetraspanin antibodies is not known, limiting mechanistic interpretation of these studies.Methods:We generated a panel of monoclonal antibodies (mAbs) specific for CD81 second extracellular domain (EC2) and performed detailed epitope mapping with a panel of CD81 mutants. All mAbs were screened for their ability to inhibit HCV infection and E2-CD81 association. Nanoscale distribution of cell surface CD81 was investigated by scanning electron
Background:Tetraspanins are small transmembrane proteins, found in all higher eukaryotes, that compartmentalize cellular membranes through interactions with partner proteins. CD81 is a prototypical tetraspanin and contributes to numerous physiological and pathological processes, including acting as a critical entry receptor for hepatitis C virus (HCV). Antibody engagement of tetraspanins can induce a variety of effects, including actin cytoskeletal rearrangements, activation of MAPK-ERK signaling and cell migration. However, the epitope specificity of most anti-tetraspanin antibodies is not known, limiting mechanistic interpretation of these studies.Methods:We generated a panel of monoclonal antibodies (mAbs) specific for CD81 second extracellular domain (EC2) and performed detailed epitope mapping with a panel of CD81 mutants. All mAbs were screened for their ability to inhibit HCV infection and E2-CD81 association. Nanoscale distribution of cell surface CD81 was investigated by scanning electron
Background: Tetraspanins are small transmembrane proteins, found in all higher eukaryotes, that compartmentalize cellular membranes through interactions with partner proteins. CD81 is a prototypical tetraspanin and contributes to numerous physiological and pathological processes, including acting as a critical entry receptor for hepatitis C virus (HCV). Antibody engagement of tetraspanins can induce a variety of effects, including actin cytoskeletal rearrangements, activation of MAPK-ERK signaling and cell migration. However, the epitope specificity of most anti-tetraspanin antibodies is not known, limiting mechanistic interpretation of these studies. Methods: We generated a panel of monoclonal antibodies (mAbs) specific for CD81 second extracellular domain (EC2) and performed detailed epitope mapping with a panel of CD81 mutants. All mAbs were screened for their ability to inhibit HCV infection and E2-CD81 association. Nanoscale distribution of cell surface CD81 was investigated by scanning electron
With various integrin-tetraspanin combinations being described in different cell types, the main challenge remains for us to understand the structural basis of all these interactions. In particular, it will be important to establish whether the regions that are engaged in the α3β1-CD151 interaction also form the contact interface of other integrin-tetraspanin pairs. Furthermore, what is the hierarchical order of the interactions between integrin-proximal CD151-CD81 and other tetraspanins? A detailed pair-wise analysis of various tetraspanin-tetraspanin interactions should determine the spatial organisation of integrin-tetraspanin clusters and set up a structural framework for future functional analyses. An important aspect of this work will be to establish which part(s) of tetraspanins controls their association with PtdIns 4-K and PKC isoforms. Current data pose a number of intriguing questions for the future. Firstly, why is PtdIns 4-K-associated activity not detected in the ...
Tetraspanins are family of small membrane proteins and they are involved in multitude of biological process. Structurally theyare characterized by having four transmembrane domains, short inner and outer loops, one large extra cellular loop containsCCG motif and N and C terminal. Iconic features of these proteins are formation of Tetraspanin Enriched Micro domains(TEMs) by interacting among themselves and with other transmembrane and cytosolic proteins. These domains provide asignaling platform for many important cellular functions such as immune response induction, fertilization, viral infection,maintenance of skin integrity and malignant process. Tetraspanin CD151 is frequently over expressed on cancer cells and isfunctionally linked to cancer metastasis. CD151 forms direct and stable and interaction with integrin molecules and regulatesthe cellular functions. Increasing evidence emerging from in vitro, in vivo and clinical analyses associates that CD151partnership with integrins ?6?1 and ...
Tetraspanins are membranes proteins involved in every aspect of cell-cell interaction, including adhesion, fusion, differentiation and extracellular vesicle production. As the principal components of a type of membrane microdomain, they have therapeutic and diagnostic potential in a wide range of diseases and new imaging techniques are beginning to reveal the fine detail of these microdomains and their biophysical properties. In this Focussed Meeting, the roles of tetraspanins in cancer, development, infection and immunity will be highlighted. In addition, the role of tetraspanins in the formation and function of extracellular vesicles will be explored and an optional half-day workshop will be held, providing hands-on experience of vesicle purification and characterisation. ... [Information of the supplier] ...
Pronephric cilia in ocrl-/- zebrafish.A. Confocal images of pronephric cilia, detected using anti-acetylated tubulin antibody, in wild-type, ocrl-/- mutant, con
Used to detect a broad range of pyrogens in parenteral products including pharmaceuticals, biopharmaceuticals or medical devices, the monocyte...
Title: Investigating the molecular mechanism of COPD in tetraspanin CD9/CD81 DKO mice- a new model for ageing. 6/9 Yuko Tsuchiya. 6/16 Special seminar 15:00 ...
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Clone FR3-16A11 recognizes CD203c, a glycosylated type II transmembrane molecule that belongs to the family of ecto-nucleotide pyrophosphatase/ phosphodiesterase (E-NPP3) enzymes. Among hematopoietic cells, expression of CD203c is restricted to basophils as well as to mast cells and their precursors, and has been described as specific for this lineage. Protein and/or mRNA expression of CD203c has also been found in solid tissues such as uterus or prostate. Basophils and mast cells are key producers of mediators that drive the onset of inflammatory responses, e.g., in allergy. Allergen challenge leads to a rapid up-regulation of activation markers such as CD203c or CD63. Due to its restricted expression pattern, CD203c is discussed as a specific marker to monitor the allergen-induced activation of basophils, e.g., in flow cytometric basophil activation tests of the peripheral blood. - Great Britain
Allergic diseases affect approximately 30% of adults and has an impact on both the individuals quality of life as well as an economic impact on society. Two effector cells involved in allergic disease are mast cells and basophils, where basophils are more readily available in blood and therefore of great interest when studying allergy. Basophils can be recruited into the tissue during inflammation originating from for example allergic reactions or parasite infections. Allergy diagnostics starts with evaluation of the patients medical history followed by in vivo and/or in vitro testing. All diagnostic tests have different advantages and disadvantages are chosen depending on the patient and the circumstances. In vivo tests include the gold standard of allergy diagnostics, which is the challenge tests, but also the commonly used skin prick test (SPT). Allergy diagnostics can also be done in vitro using allergen-specific IgE antibody assays and the basophil activation test (BAT). BAT is useful to ...
Purpose of review Mechanisms involved in the development of type 2 immunity are poorly defined. In addition progenitors that differentiate into mature basophils have recently been recognized. Summary The current review revisits basophils with the goal of providing insights into understanding unappreciated functions of basophils studies of human basophils have provided insights into understanding basophil […]. ...
E xosomes are a discrete population of vesicles that are secreted from various cell types to the extracellular media. Characterization of exosomes from differ...
Exosome onderzoek Isolatie , Detectie , Ontdekking , Diensten Exosome-isolatie op uitzonderlijk niveau van zuiverheid en opbrengst ExoQuick® ULTRA / ExoQuick®-TC ULTRA EV-isolatiekits voor de zuivering van hoogwaardige extracellulaire blaasjes…. ...
The tetraspanin superfamily proteins play important roles in organizing membrane protein complexes, modulating integrin function, and controlling T cell adhesion. Tetraspanins such as CD82 contain two extracellular loops with its N terminus, C terminus, and inner loop exposed to the cytoplasm. The m …
Clone FR3-16A11 recognizes CD203c, a glycosylated type II transmembrane molecule that belongs to the family of ecto-nucleotide pyrophosphatase/ phosphodiesterase (E-NPP3) enzymes. Among hematopoietic cells, expression of CD203c is restricted to basophils as well as to mast cells and their precursors, and has been described as specific for this lineage. Protein and/or mRNA expression of CD203c has also been found in solid tissues such as uterus or prostate. Basophils and mast cells are key producers of mediators that drive the onset of inflammatory responses, e.g., in allergy. Allergen challenge leads to a rapid up-regulation of activation markers such as CD203c or CD63. Due to its restricted expression pattern, CD203c is discussed as a specific marker to monitor the allergen-induced activation of basophils, e.g., in flow cytometric basophil activation tests of the peripheral blood. - Lëtzebuerg
Plasma exchange to remove HIT antibodies: dissociation between enzyme-immunoassay and platelet activation test reactivities Academic Article ...
Hypersensitivity reactions to betalactams (BLs) are classified as immediate or nonimmediate. The former usually appear within 1 h of drug-intake and are mediated by specific IgE-antibodies. Nonimmediate reactions are those occurring more than 1 h after drug-intake, and they can be T-cell mediated. The diagnostic evaluation of allergic reactions to BLs has changed over the last 5 years, for several reasons. Major and minor determinants are no longer commercially available for skin testing in many countries. In immediate allergic reactions, the sensitivity of skin testing and immunoassays is decreasing and new in vitro methods, such as the basophil activation test, are gaining importance for diagnosis. For nonimmediate reactions, skin testing appears to be less sensitive than previous results, although more studies need to be carried out in this direction. Nevertheless, the drug provocation test is still necessary for diagnosis. ...
Basophils are a rare granulocyte population that has been associated with allergic and inflammatory responses. It is essential to understand the regulatory mechanisms by which basophils are kept in check, considering the impact of dysregulated basophil function on immune responses under different pathological conditions. Among immunoregulatory cells, CD4+CD25+FoxP3+ regulatory T cells (Tregs) are the key players that maintain immune tolerance. The mechanisms by which Tregs regulate and suppress diverse immune cell subsets have been studied extensively, but the impact of Tregs on basophil functions is not well understood. We report that human basophils are refractory to Treg-mediated suppression and found that Tregs stimulate resting basophils to induce the expression of activation markers including CD69, CD203c, and CD13 and the release of basophil cytokines including IL-13, IL-8, and IL-4. Mechanistically, Tregs could induce human basophil activation via IL-3 and STAT5 activation, whereas ...
Previous research suggests that human basophil activation may be inhibited by histamine even at extremely low doses (high dilutions). However, uncertainti
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Human immunodeficiency virus type 1 (HIV-1) transmission takes place primarily through cell-cell contacts known as virological synapses. Formation of these transient adhesions between infected and uninfected cells can lead to transmission of viral particles followed by separation of the cells. Alternatively, the cells can fuse, thus forming a syncytium. Tetraspanins, small scaffolding proteins that are enriched in HIV-1 virions and actively recruited to viral assembly sites, have been found to negatively regulate HIV-1 Env-induced cell-cell fusion. How these transmembrane proteins inhibit membrane fusion, however, is currently not known. As a first step towards elucidating the mechanism of fusion repression by tetraspanins, e.g., CD9 and CD63, we sought to identify the stage of the fusion process during which they operate. Using a chemical epistasis approach, four fusion inhibitors were employed in tandem with CD9 overexpression. Cells overexpressing CD9 were found to be sensitized to inhibitors
Tetraspanins function as molecular organizers of multi-protein complexes by assembling primary complexes of a relatively low mass into extensive networks involved in cellular signalling. In this paper, we summarize our studies performed on the tetraspanin D6.1A/CO-029/TM4SF3 expressed by rat carcinoma cells. Primary complexes of D6.1A are almost indistinguishable from complexes isolated with anti-CD9 antibody. Indeed, both tetraspanins directly associate with each other and with a third tetraspanin, CD81. Moreover, FPRP (prostaglandin F2α receptor-regulatory protein)/EWI-F/CD9P-1), an Ig superfamily member that has been described to interact with CD9 and CD81, is also a prominent element in D6.1A complexes. Primary complexes isolated with D6.1A-specific antibody are clearly different from complexes containing the tetraspanin CD151. CD151 is found to interact only with D6.1A if milder conditions, i.e. lysis with LubrolWX instead of Brij96, are applied to disrupt cellular membranes. CD151 ...
Supplementary MaterialsData_Sheet_1. by looking at with healthy individuals, higher levels of serum exosomal miR-92b-3p, let-7g-5p, miR-146b-5p, and miR-9-5p were found to be significantly associated with early-stage GC ( 0.05). Diagnostic power of the combined panels of the exosomal miRNAs or the combination of exosomal BAY 80-6946 supplier miRNAs and CEA outperformed that of single exosomal miRNA marker for establishing a diagnosis of early-stage GC. The combined diagnosis of exosomal miR-92b-3p + let-7g-5p + miR-146b-5p + miR-9-5p with CEA had the most powerful efficiency with an AUC up to 0.786. In addition, serum levels of exosomal miR-92b-3p were significantly associated with poor cohesiveness (= 0.0021), let-7g-5p and miR-146b-5p were significantly correlated with nerve infiltration (= 0.0234 and = 0.0126, respectively), and miR146b-5p was statistically correlated with tumor invasion depth in early-stage GC (= 0.0089). In conclusion, serum exosomal miR-92b-3p, -146b-5p, -9-5p, and ...
Thomas Schneider is the author of these articles in the Journal of Visualized Experiments: Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity, Quasi-light Storage for Optical Data Packets, Studying Soft-matter and Biological Systems over a Wide Length-scale from Nanometer and Micrometer Sizes at the Small-angle Neutron Diffractometer KWS-2
UK-based Wickham Laboratories has introduced the monocyte activation test (MAT) as the latest addition to its portfolio of in vitro alternatives to in vivo testing methods.
human CD231 antigen: tetraspanin protein; has the unique potential to modulate HIV-1 infectivity through incorporation into released HIV-1 particles
What are exosomes, and why are they becoming so relevant in the medical and pharmaceutical field? Read all about exosomes and their versatility here.
The CD63 marker is a fluorescein isothiocyanate labeled antigen which can bind to an CD63 protein and is used to sort the cells ... the expression of the CD63 antigen on the cell surface (plasma membrane) allows identification of the allergen responsible for ... After degranulation a CD63 marker (labeled antibodies) is added to the test tube. Several minutes at room temperature gives the ... It can be used for different allergies (e.g. bee venom, drugs, contrast media). Degranulated cell expose CD63 molecules on ...
... has been shown to interact with CD19, CD63 and CD234. CD82 plays a key role in the development of endometriosis. ... November 1992). "C33 antigen recognized by monoclonal antibodies inhibitory to human T cell leukemia virus type 1-induced ... CD63, and CD53 specifically associated with integrin alpha 4 beta 1 (CD49d/CD29)". Journal of Immunology. 157 (5): 2039-47. ... "A new superfamily of lymphoid and melanoma cell proteins with extensive homology to Schistosoma mansoni antigen Sm23". European ...
... antigen is a protein that, in humans, is encoded by the CD63 gene. CD63 is mainly associated with membranes of ... CD63+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH) Human CD63 genome location and CD63 ... "CD63 antigen. A novel lysosomal membrane glycoprotein, cloned by a screening procedure for intracellular antigens in eukaryotic ... Hotta H, Miyamoto H, Hara I, Takahashi N, Homma M (May 1992). "Genomic structure of the ME491/CD63 antigen gene and functional ...
CD49d+antigen at the US National Library of Medicine Medical Subject Headings (MeSH) ITGA4 Info with links in the Cell ... CD63, and CD53 specifically associated with integrin alpha 4 beta 1 (CD49d/CD29)". J. Immunol. 157 (5): 2039-47. PMID 8757325. ... "Entrez Gene: ITGA4 integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)". Hadari YR, Arbel-Goren R, Levy Y, ... Takada Y, Strominger JL, Hemler ME (1987). "The very late antigen family of heterodimers is part of a superfamily of molecules ...
"The primary structure of the human leukocyte antigen CD37, a species homologue of the rat MRC OX-44 antigen". The Journal of ... CD53 and CD63". FEBS Letters. 288 (1-2): 1-4. doi:10.1016/0014-5793(91)80988-F. PMID 1879540. S2CID 26316623. Berditchevski F ( ... Leukocyte antigen CD37 is a protein that in humans is encoded by the CD37 gene. The protein encoded by this gene is a member of ... Angelisová P, Hilgert I, Horejsí V (1994). "Association of four antigens of the tetraspans family (CD37, CD53, TAPA-1, and R2/ ...
This antigen along with other blood group antigens was used to identify the Basque people as a genetically separate group.[49] ... Because the Duffy antigen is uncommon in those of Black African descent, the presence of this antigen has been used to detect ... The Fy4 antigen, originally described on Fy (a-b-) RBCs, is now thought to be a distinct, unrelated antigen and is no longer ... The Duffy antigen is expressed in greater quantities on reticulocytes than on mature erythrocytes.[21] While the Duffy antigen ...
It forms a alphaIIbbeta3-CD9-CD63 complex on the surface of platelets that interacts directly with other cells such as ... "Molecular cloning of the CD9 antigen. A new family of cell surface proteins". The Journal of Biological Chemistry. 266 (1): 117 ... CD63 ADAM17 CD81 Tetraspanin Myogenesis Fertilisation GRCh38: Ensembl release 89: ENSG00000010278 - Ensembl, May 2017 GRCm38: ... Radford KJ, Thorne RF, Hersey P (May 1996). "CD63 associates with transmembrane 4 superfamily members, CD9 and CD81, and with ...
Radford KJ, Thorne RF, Hersey P (May 1996). "CD63 associates with transmembrane 4 superfamily members, CD9 and CD81, and with ... 1994). "Mouse homologue of C33 antigen (CD82), a member of the transmembrane 4 superfamily: complementary DNA, genomic ... Radford KJ, Thorne RF, Hersey P (1996). "CD63 associates with transmembrane 4 superfamily members, CD9 and CD81, and with beta ... The tetraspanin family includes CD9, CD37, CD53, CD63, CD81 (this protein), CD82 and CD151. CD81 interacts directly with ...
CD154 knockout mice are incapable of producing IgG, IgE, or IgA as a response to antigens. Microvesicles can also transfer ... Finally, tetraspanin proteins, including CD9, CD37, CD63 and CD81 are one of the most abundant protein families found in ... This mechanism of action can be used in processes such as antigen presentation, where MHC molecules on the surface of ... For example, those released from antigen-presenting cells (APCs), such as B cells and dendritic cells, are enriched in proteins ...
1990). "The human leucocyte surface antigen CD53 is a protein structurally similar to the CD37 and MRC OX-44 antigens". ... 1998). "Expression of tetra-spans transmembrane family (CD9, CD37, CD53, CD63, CD81 and CD82) in normal and neoplastic human ... Leukocyte surface antigen CD53 is a protein that in humans is encoded by the CD53 gene. The protein encoded by this gene is a ... A pan-leukocyte antigen related to membrane transport proteins". J. Immunol. 145 (12): 4322-5. PMID 2258620. Dianzani U, ...
"Molecular cloning of cDNA for the human tumor-associated antigen CO-029 and identification of related transmembrane antigens". ... Gwynn B, Eicher EM, Peters LL (July 1996). "Genetic localization of Cd63, a member of the transmembrane 4 superfamily, reveals ...
Raph blood group system in the BGMUT blood group antigen gene mutation database Human CD151 genome location and CD151 gene ... CD63, and alpha5beta1 integrin". J. Histochem. Cytochem. 45 (4): 515-25. doi:10.1177/002215549704500404. PMID 9111230. Suzuki Y ... identifies a novel platelet surface antigen". Br. J. Haematol. 79 (2): 263-70. doi:10.1111/j.1365-2141.1991.tb04531.x. PMID ... "Molecular cloning of cDNA encoding a novel platelet-endothelial cell tetra-span antigen, PETA-3". Blood. 86 (4): 1348-55. doi: ...
Subrahmanyam G, Rudd CE, Schneider H (2003). "Association of T cell antigen CD7 with type II phosphatidylinositol-4 kinase, a ... Israels SJ, McMillan-Ward EM (2005). "CD63 modulates spreading and tyrosine phosphorylation of platelets on immobilized ... Israels SJ, McMillan-Ward EM (2005). "CD63 modulates spreading and tyrosine phosphorylation of platelets on immobilized ...
Recently, Heneberg proposed that basophils may be defined as the cellular population positive for CD13, CD44, CD54, CD63, CD69 ... pollen proteins or helminth antigens. Recent studies in mice suggest that basophils may also regulate the behavior of T cells ... When activated, some additional surface markers are known to be upregulated (CD13, CD107a, CD164), or surface-exposed (CD63, ...
CD29+Antigen at the US National Library of Medicine Medical Subject Headings (MeSH) Human ITGB1 genome location and ITGB1 gene ... Radford KJ, Thorne RF, Hersey P (May 1996). "CD63 associates with transmembrane 4 superfamily members, CD9 and CD81, and with ... These and other integrin beta 1 complexes have been historically known as very late activation (VLA) antigens. Integrin beta 1 ... "Entrez Gene: ITGB1 integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)". Hynes RO (Apr ...
2002). "Flow cytometric basophil activation test by detection of CD63 expression in patients with immediate-type reactions to ... leukocyte histocompatibility antigen (HLA group) typing for organ transplant (mainly kidney and liver), as well as cellular ...
Tissue Antigens (англ.)русск. : journal. - 2007. - Vol. 68, no. 6. - P. 509-517. - DOI:10.1111/j.1399-0039.2006.00726.x. - PMID ... CD63. *CD64 (A, B, C). *CD66 (a, b, c, d, e, f) ...
CD51 • CD52 • CD53 • CD54 • CD55 • CD56 • CD57 • CD58 • CD59 • CD61 • CD62 (E, L, P) • CD63 • CD64 (A, B, C) • CD66 (a, b, c, d ... CD97 antigen je protein koji je kod ljudi kodiran CD97 genom.[1][2][3] ... 2001). „Tissue distribution of the human CD97 EGF-TM7 receptor". Tissue Antigens. 57 (4): 325-31. PMID 11380941. doi:10.1034/j. ... Expression cloning and chromosomal mapping of the leukocyte activation antigen CD97, a new seven-span transmembrane molecule of ...
1997). "The Oka blood group antigen is a marker for the M6 leukocyte activation antigen, the human homolog of OX-47 antigen, ... 1992). "Human leukocyte activation antigen M6, a member of the Ig superfamily, is the species homologue of rat OX-47, mouse ... Kasinrerk W, Fiebiger E, Stefanová I, Baumruker T, Knapp W, Stockinger H (1992). "Human leukocyte activation antigen M6, a ... Ok blood group system at BGMUT Blood Group Antigen Gene Mutation Database at NCBI, NIH ...
Seligman P. A., Butler C. D., Massey E. J., etal. The p97 antigen is mapped to the q24-qter region of chromosome 3; the same ... CD63. *CD64 (A, B, C). *CD66 (a, b, c, d, e, f) ... Richardson D. R. The role of the membrane-bound tumour antigen ... Le Beau M. M., Diaz M. O., Plowman G. D., etal. Chromosomal sublocalization of the human p97 melanoma antigen. (англ.) // Hum. ... Plowman G. D., Brown J. P., Enns C. A., etal. Assignment of the gene for human melanoma-associated antigen p97 to chromosome 3 ...
CD74 (англ. HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain) - мембранный белок ... CD63. *CD64 (A, B, C). *CD66 (a, b, c, d, e, f) ... antigensIiHLA-DR antigens-associated invariant chainIa antigen- ... Riberdy J.M., Newcomb J.R., Surman M.J., Barbosa J.A., Cresswell P. HLA-DR molecules from an antigen-processing mutant cell ... Machamer C.E., Cresswell P. Biosynthesis and glycosylation of the invariant chain associated with HLA-DR antigens (англ.) // ...
CD51 • CD52 • CD53 • CD54 • CD55 • CD56 • CD57 • CD58 • CD59 • CD61 • CD62 (E, L, P) • CD63 • CD64 (A, B, C) • CD66 (a, b, c, d ... 1991). „Expression of the YB5.B8 antigen (c-kit proto-oncogene product) in normal human bone marrow". Blood. 78 (1): 30-7. PMID ... 2003). „Signal transduction-associated and cell activation-linked antigens expressed in human mast cells". Int. J. Hematol. 75 ...
December 1984). "The neu oncogene: an erb-B-related gene encoding a 185,000-Mr tumour antigen". Nature. 312 (5994): 513-16. ... like TOP1 and a glycosylated form of tetraspanin CD63 involved in organelle structure, cytokinesis and response to lipids. " ... "Inhibition of tumor growth by a monoclonal antibody reactive with an oncogene-encoded tumor antigen". Proceedings of the ... "Monoclonal antibodies identify a cell-surface antigen associated with an activated cellular oncogene". Nature. 312 (5994): 545- ...
... is a co-receptor of the T cell receptor (TCR) and assists the latter in communicating with antigen-presenting cells. The ... Leucocyte typing: human leucocyte differentiation antigens detected by monoclonal antibodies: specification, classification, ... T cells displaying CD4 molecules (and not CD8) on their surface, therefore, are specific for antigens presented by MHC II and ... CD1+Antigen at the US National Library of Medicine Medical Subject Headings (MeSH) ...
In addition to aiding with cytotoxic T cell antigen interactions the CD8 co-receptor also plays a role in T cell signaling. The ... the CD8 co-receptor plays a role in T cell signaling and aiding with cytotoxic T cell antigen interactions. ... This affinity keeps the T cell receptor of the cytotoxic T cell and the target cell bound closely together during antigen- ... Once the T cell receptor binds its specific antigen Lck phosphorylates the cytoplasmic CD3 and ζ-chains of the TCR complex ...
In humans, the CD44 antigen is encoded by the CD44 gene on Chromosome 11.[5] CD44 has been referred to as HCAM (homing cell ... The CD44 antigen is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. ... Indian blood group system at BGMUT Blood Group Antigen Gene Mutation Database at NCBI, NIH ... "Carcinoembryonic antigen and CD44 variant isoforms cooperate to mediate colon carcinoma cell adhesion to E- and L-selectin in ...
CD51 • CD52 • CD53 • CD54 • CD55 • CD56 • CD57 • CD58 • CD59 • CD61 • CD62 (E, L, P) • CD63 • CD64 (A, B, C) • CD66 (a, b, c, d ... 1996). "CD88 antibodies specifically bind to C5aR on dermal CD117+ and CD14+ cells and react with a desmosomal antigen in human ...
van Rhenen A., van Dongen G. A., Kelder A., et al. The novel AML stem cell associated antigen CLL-1 aids in discrimination ... CD63. *CD64 (A, B, C). *CD66 (a, b, c, d, e, f) ...
A new ligand for human leukocyte antigen class II antigens". The Journal of Experimental Medicine. 176 (2): 327-37. doi:10.1084 ... A new ligand for human leukocyte antigen class II antigens". The Journal of Experimental Medicine. 176 (2): 327-37. doi:10.1084 ... antigen processing and presentation of exogenous peptide antigen via MHC class II. ... antigen binding. • transmembrane signaling receptor activity. • MHC class II protein binding. Cellular component. • membrane. • ...
Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) also known as CD66e (Cluster of Differentiation 66e), is a ... 2001). "Heterogeneous RNA-binding protein M4 is a receptor for carcinoembryonic antigen in Kupffer cells". J. Biol. Chem. 276 ( ... CEACAM5, CD66e, CEA, carcinoembryonic antigen related cell adhesion molecule 5. External IDs. HomoloGene: 128801 GeneCards: ... Oikawa S, Nakazato H, Kosaki G (1987). "Primary structure of human carcinoembryonic antigen (CEA) deduced from cDNA sequence". ...
CD51 • CD52 • CD53 • CD54 • CD55 • CD56 • CD57 • CD58 • CD59 • CD61 • CD62 (E, L, P) • CD63 • CD64 (A, B, C) • CD66 (a, b, c, d ... 2000). "Characterization of a new member of the TNF family expressed on antigen presenting cells.". Biol. Chem. 380 (12): 1443- ... "BLyS receptor signatures resolve homeostatically independent compartments among naïve and antigen-experienced B cells.". Semin ...
I. Partial characterization of soluble Ki-1 antigen and detection of the antigen in cell culture supernatants and in serum by ... Josimovic-Alasevic O, Dürkop H, Schwarting R, Backé E, Stein H, Diamantstein T (Jan 1989). "Ki-1 (CD30) antigen is released by ... CD30+Antigens at the US National Library of Medicine Medical Subject Headings (MeSH) ... results from cDNA cloning and sequence comparison of the CD30 antigen from different sources". Molecular Immunology. 31 (17): ...
Macrophage-1 antigen (CD11b+CD18). *VLA-4 (CD49d+CD29). *Glycoprotein IIb/IIIa (ITGA2B+ITGB3) ...
The protein also carries the Jr(a) antigen, which defines the Junior blood group system.[9] ...
"Interaction of glycogen synthase kinase 3beta with the DF3/MUC1 carcinoma-associated antigen and beta-catenin". Molecular and ...
Ebert LM, McColl SR (2002). "Up-regulation of CCR5 and CCR6 on distinct subpopulations of antigen-activated CD4+ T lymphocytes ... This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may ... dendritic cells induce antitumor immunity when genetically fused with nonimmunogenic tumor antigens". J. Immunol. 167 (11): ...
It is also called Lewis x and SSEA-1 (stage-specific embryonic antigen 1) and represents a marker for murine pluripotent stem ... CD15 Antigen at the US National Library of Medicine Medical Subject Headings (MeSH) ... CD15 (3-fucosyl-N-acetyl-lactosamine) is a cluster of differentiation antigen - an immunologically significant molecule. CD15 ...
CD51 • CD52 • CD53 • CD54 • CD55 • CD56 • CD57 • CD58 • CD59 • CD61 • CD62 (E, L, P) • CD63 • CD64 (A, B, C) • CD66 (a, b, c, d ... CD97 antigen je protein koji je kod ljudi kodiran CD97 genom.[1][2][3] ... 2001). "Tissue distribution of the human CD97 EGF-TM7 receptor". Tissue Antigens 57 (4): 325-31. PMID 11380941. doi:10.1034/j. ... "Expression cloning and chromosomal mapping of the leukocyte activation antigen CD97, a new seven-span transmembrane molecule of ...
"Entrez Gene: ITGB3 integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)".. *^ May, K. E.; Villar, J.; Kirtley, S.; ... CD61+Antigens at the US National Library of Medicine Medical Subject Headings (MeSH) ...
"Direct association of adenosine deaminase with a T cell activation antigen, CD26". Science. 261 (5120): 466-9. doi:10.1126/ ...
B cells can present antigens to a specialized group of helper T cells called TFH cells. If an activated TFH cell recognizes the ... Roles of T cell-B-cell-activating molecule (5c8 antigen) and CD40 in contact-dependent help". Journal of Immunology. 149 (12): ... It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In ... Grewal, IS; Xu, J; Flavell, RA (7 December 1995). "Impairment of antigen-specific T-cell priming in mice lacking CD40 ligand". ...
antigen processing and presentation of peptide antigen via MHC class I. • antigen processing and presentation of exogenous ... CD63. *CD64 (A, B, C). *CD66 (a, b, c, d, e, f) ... peptide antigen via MHC class I, TAP-dependent. • platelet ... antigen processing and presentation of exogenous peptide antigen via MHC class I. • lipoprotein transport. • negative ...
Primarily, the VCAM-1 protein is an endothelial ligand for VLA-4 (Very Late Antigen-4 or integrin α4β1) of the β1 subfamily of ...
... uveitis antigens induce CXCR3- and CXCR5-expressing lymphocytes and immature dendritic cells to migrate (англ.) // Blood (англ ... CD63. *CD64 (A, B, C). *CD66 (a, b, c, d, e, f) ...
antigen binding. • virus receptor activity. • protein binding. • transmembrane signaling receptor activity. • identical protein ...
Recently, Heneberg[17] proposed that basophils may be defined as the cellular population positive for CD13, CD44, CD54, CD63, ... pollen proteins or helminth antigens. Recent studies in mice suggest that basophils may also regulate the behavior of T cells ... When activated, some additional surface markers are known to be upregulated (CD13, CD107a, CD164), or surface-exposed (CD63, ...
Expression of the YB5.B8 antigen (c-kit proto-oncogene product) in normal human bone marrow. », Blood, vol. 78, no 1,‎ 1991. , ... CD63 (en)[10],. *CD81 (en)[10],. *CD9 (en)[10],. *CRK (gene) (en)[11],[11], ... Signal transduction-associated and cell activation-linked antigens expressed in human mast cells. », Int. J. Hematol., vol. 75 ...
T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell. • T cell antigen ... CD51 · CD52 · CD53 · CD54 · CD55 · CD56 · CD57 · CD58 · CD59 · CD61 · CD62 (E, L, P) · CD63 · CD64 (A, B, C) · CD66 (a, b, c, d ...
CD63 antigen isoform X1 [Homo sapiens] CD63 antigen isoform X1 [Homo sapiens]. gi,1370463253,ref,XP_024305051.1, ... Altered expression of CD63 and exosomes in scleroderma dermal fibroblasts. [J Dermatol Sci. 2016] Altered expression of CD63 ... The CD63 gene is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, zebrafish, fruit fly, mosquito, C.elegans, and ...
sp,P41731,CD63_MOUSE CD63 antigen OS=Mus musculus OX=10090 GN=Cd63 PE=1 SV=2 ... "CD63 is an essential cofactor to leukocyte recruitment by endothelial P-selectin.". Doyle E.L., Ridger V., Ferraro F., Turmaine ... "CD63 is an essential cofactor to leukocyte recruitment by endothelial P-selectin.". Doyle E.L., Ridger V., Ferraro F., Turmaine ... "The tetraspanin CD63 is required for efficient IgE-mediated mast cell degranulation and anaphylaxis.". Kraft S., Jouvin M.H., ...
sp,Q28709,CD63_RABIT CD63 antigen OS=Oryctolagus cuniculus OX=9986 GN=CD63 PE=2 SV=2 ... CD63_RABIT. ,p>This subsection of the Entry information section provides one or more accession number(s). These are stable ...
CD63" by people in this website by year, and whether "Antigens, CD63" was a major or minor topic of these publications. ... "Antigens, CD63" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... Below are the most recent publications written about "Antigens, CD63" by people in Profiles. ... Below are MeSH descriptors whose meaning is more general than "Antigens, CD63". ...
Homo sapiens,Human,CD63 antigen,Granulophysin,Lysosomal-associated membrane protein 3,LAMP-3,Melanoma-associated antigen ME491, ... Antigens are peptides or recombinant or native dependent on the production method. ...
... and melanoma-associated antigen (ME491). CD63 is a member of the tetraspan transmembrane superfamily (TM4SF) protein expressed ... p,CD63 is a 53 kD type III lysosomal glycoprotein also known as LIMP, LAMP-3, gp55, ... CD63 is a 53 kD type III lysosomal glycoprotein also known as LIMP, LAMP-3, gp55, and melanoma-associated antigen (ME491). CD63 ... CD63 may be involved in platelet activation and is thought to function as a transmembrane adaptor protein. CD63 has been shown ...
CD63 is a 53 kD type III lysosomal glycoprotein also known as LIMP, LAMP-3, gp55, and melanoma-associated antigen (ME491). ... CD63 is a 53 kD type III lysosomal glycoprotein also known as LIMP, LAMP-3, gp55, and melanoma-associated antigen (ME491). CD63 ... CD63 may be involved in platelet activation and is thought to function as a transmembrane adaptor protein. CD63 has been shown ... Antigen References *Azorsa DO, et al. 1991. Blood 78:280.. *Kishimoto T, et al. Eds. 1997. Leukocyte Typing V1. Oxford ...
... products and learn more about CD63 Mouse anti-Human, Brilliant Violet 510, Clone: H5C6, BD Optibuild 50µg; Brilliant Violet ... This molecule is also referred to in the literature as LIMP, gp55, melanoma-associated antigen ME491, Pltgp40, LAMP-3 and is a ... The H5C6 monoclonal antibody specifically binds to CD63. CD63 is a 53 kDa, type III lysosomal glycoprotein, expressed on ... CD63 plays roles in mediating cellular adhesion and motility. The antibody was conjugated to BD Horizon BV510 which is part of ...
... products and learn more about CD63 Mouse anti-Human, PerCP-Cy5.5, Clone: H5C6, BD 50 Tests; PerCP-Cy5.5:Life 50 Tests; PerCP- ... This molecule is also referred to in the literature as LIMP, gp55, melanoma-associated antigen ME491, Pltgp40, LAMP-3 and is a ... The H5C6 monoclonal antibody specifically binds to CD63. CD63 is a 53 kDa, type III lysosomal glycoprotein, expressed on ...
CD 63 antigen. A novel lysosomal membrane glycoprotein, cloned by a screening procedure for intracellular antigens in in ...
Our double-immunogold staining for the exosome surface protein CD63 with either of the transgenes revealed that CD63 and the ... A role for intercellular antigen transfer in the recognition of EBV-transformed B cell lines by EBV nuclear antigen-specific ... One drawback of our model with retrovirally transduced antigen is the induced overexpression of the target antigen. High ... Therefore, it remains controversial whether intercellular antigen transfer is a sole consequence of cell death-mediated antigen ...
CD63 antigen is a protein that, in humans, is encoded by the CD63 gene. CD63 is mainly associated with membranes of ... CD63+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH) Human CD63 genome location and CD63 ... "CD63 antigen. A novel lysosomal membrane glycoprotein, cloned by a screening procedure for intracellular antigens in eukaryotic ... Hotta H, Miyamoto H, Hara I, Takahashi N, Homma M (May 1992). "Genomic structure of the ME491/CD63 antigen gene and functional ...
Mouse Monoclonal Anti-CD63 Antibody (LAMP3/968) [Janelia Fluor® 549]. Late Endosomes Marker. Validated: Flow, IHC, IHC-P, ICC/ ... Alternate Names for CD63 Antibody (LAMP3/968) [Janelia Fluor® 549]. *CD63 antigen (melanoma 1 antigen) ... Blogs on CD63. Check out the latest blog posts on CD63.. Tools for Isolation, Quantification and Analysis of Exosomes. Exosomes ... CD63: is it pro-metastatic or anti-metastatic?. CD63 is a type II membrane protein belonging to tetraspanin superfamily and it ...
Mouse Monoclonal Anti-CD63 Antibody (MX-49.129.5) [DyLight 488]. Late Endosomes Marker. Validated: WB, ELISA, Flow, ICC/IF, IHC ... Alternate Names for CD63 Antibody (MX-49.129.5) [DyLight 488]. *CD63 antigen (melanoma 1 antigen) ... Blogs on CD63. Check out the latest blog posts on CD63.. Tools for Isolation, Quantification and Analysis of Exosomes. Exosomes ... Reviews for CD63 Antibody (NBP2-34689G) (0) There are no reviews for CD63 Antibody (NBP2-34689G). By submitting a review you ...
CD63 antigen is a protein that in humans is encoded by the CD63 gene.[1] ... "CD63 antigen. A novel lysosomal membrane glycoprotein, cloned by a screening procedure for intracellular antigens in eukaryotic ... CD51 · CD52 · CD53 · CD54 · CD55 · CD56 · CD57 · CD58 · CD59 · CD61 · CD62 (E, L, P) · CD63 · CD64 (A, B, C) · CD66 (a, b, c, d ... Hotta H, Miyamoto H, Hara I, et al. (1992). "Genomic structure of the ME491/CD63 antigen gene and functional analysis of the 5 ...
CD63 antigen. C cont.. *Allergic conjunctivitis. D. *Drug desensitization. F. *Food hypersensitivity ...
Antigens CD63. Basophil degranulation test/methods. Basophils. Drug hypersensitivity/blood. Drug hypersensitivity/diagnosis. ... Antígenos CD63. Antiinflamatórios não esteróides. Basófilos. Curva ROC. Hipersensibilidade a drogas/diagnóstico. ... BAT consisted of incubating whole blood with NSAIDs, then triple-labeled with monoclonal antibodies (CD45, anti-IgE, CD63) for ... anti-IgE e CD63) para posterior leitura por citometria de fluxo. Os resultados obtidos foram comparados com as histórias ...
Rabbit polyclonal CD63 antibody. Validated in WB, ICC/IF and tested in Human. Cited in 8 publication(s). Immunogen ... This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. ... Anti-CD63 antibody (ab118307) at 1 µg/ml + OVCAR-3 cell lysate at 10 µg. Predicted band size: 26 kDa. Gel concentration:12% ... CD63 antigen (melanoma 1 antigen) antibody. *CD63 antigen antibody. *CD63 antigen melanoma 1 antigen antibody ...
Jetzt diesen anti-CD63 Antikörper bestellen. , Produkt ABIN4261694 ... Maus Monoklonal CD63 Antikörper für ELISA, FACS, ICC, IF, WB. ... Antigen CD63 Molecule (CD63) Synonyme für dieses Antigen ... anti-CD63 Antikörper (CD63 Molecule) (DyLight 755) CD63 Antikörper (CD63 Molecule) (DyLight 755). Details for Product anti-CD63 ... Produktdetails anti-CD63 Antikörper Handhabung Anwendungsinformationen Antigendetails zurück nach oben Produktdetails anti-CD63 ...
Cd63. CD63 antigen. NM_001042580. NM_007653. Gene Info. Cdh13. Cadherin 13. NM_019707. Gene Info. ... CD300A antigen. NM_170758. Gene Info. Cd36. CD36 antigen. NM_001159558. NM_007643. NM_001159555. NM_001159557. NM_001159556. ... CD47 antigen (Rh-related antigen, integrin-associated signal transducer). NM_010581. Gene Info. ...
MHC class I antigen K06751 MHC1; MHC class I antigen K06751 MHC1; MHC class I antigen K06460 CD9; CD9 antigen K06497 CD63; CD63 ... K06751 MHC1; MHC class I antigen K06751 MHC1; MHC class I antigen K06751 MHC1; MHC class I antigen K06751 MHC1; ... 967 CD63; CD63 molecule 975 CD81; CD81 molecule 3688 ITGB1; integrin subunit beta 1 4240 MFGE8; milk fat globule EGF and factor ... antigen K06508 CD81; CD81 antigen K05719 ITGB1; integrin beta 1 K17253 MFGE8; lactadherin K06490 ICAM1; intercellular adhesion ...
... platelet K06497 CD63; CD63 antigen K06498 FCGR1A; high affinity immunoglobulin gamma Fc receptor I K06499 CEACAM; ... CD79A antigen K06507 CD79B; CD79B antigen K05412 CD80; CD80 antigen K06508 CD81; CD81 antigen K06509 KAI1; CD82 antigen K06510 ... CD300 antigen K06719 CD300; CD300 antigen K06719 CD300; CD300 antigen K06719 CD300; CD300 antigen K06721 CLEC10A; C-type lectin ... CD96 antigen K08446 ADGRE5; CD97 antigen K06519 SLC3A2; solute carrier family 3, member 2 K06520 CD99; CD99 antigen K06521 ...
CD9, CD9 antigen; CD63, CD63 antigen; Exo, exosomal; MFI, mean fluorescence intensity; PHM, primary human macrophages; Rab5B, ... CD9, CD63 and Rab5B, which are used as exosomal markers, were expressed on the THP-1- and PHM-derived exosomes (Fig. 1A). ... ab92726; Abcam), CD63 (1:1,000; cat. no. sc-5275; Santa Cruz Biotechnology, Inc.), Ras-related protein Rab-5B (Rab5B; 1:1,000; ...
Interaction of CD63 with other membrane proteins or adaptors regulates cell activities such as adhesion, migration, and ... CD63 is expressed on monocytes, macrophages, endothelium, fibroblasts, osteoclasts, smooth muscle cells, and activated ... Clone REA563 recogizes the mouse CD63 antigen. It belongs to the tetraspanin transmembrane 4 superfamily (TM4SF) of proteins, ... Clone REA563 recogizes the mouse CD63 antigen. It belongs to the tetraspanin transmembrane 4 superfamily (TM4SF) of proteins, ...
... a multi-pass membrane protein which is also known as mast cell antigen AD1 or melanoma antigen ME491. CD63 is located on the ... CD63 functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. It ... CD63 belongs to the tetraspanin transmembrane 4 superfamily (TM4SF) of proteins, which are characterized by four membrane ... Nishikata, H. et al. (1992) The rat mast cell antigen AD1 (homologue to human CD63 or melanoma antigen ME491) is expressed in ...
Anti-CD63 Antibody with Goat Anti-Rabbit HRP Secondary Antibody. Shop Now ... Putting exosomes to work: Target exosomes to antigen-presenting cells. Use exosomes to deliver protein, RNA, DNA, or small ... Target exosomes to antigen-presenting cells with the pre-built XStamp-CD40L Lentivector Construct. ... Home , Products , Exosome Research , Exosome Engineering , XStamp™-CD40L Lentivector for Targeting Exosomes to Antigen- ...
CD63 antigen; tspan-30; granulophysin; tetraspanin-30; melanoma-associated antigen MLA1; CD63 antigen (melanoma 1 antigen); ... CD63; CD63 molecule; MLA1; ME491; LAMP-3; OMA81H; TSPAN30; ... melanoma-associated antigen ME491; ocular melanoma-associated ... antigen; lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3; cd163; zgc:65825; zgc:77709; wu: ...
Antigen Background CD63 antigen is a member of the TM4 superfamily with its structure consisting of four transmembrane regions ... CD63 antigen associates non-covalently with CD9, CD81 and the integrins VLA-3, VLA-4 and VLA-6. It is reported that CD63 ... CD63-U. 1ml NCL-CD63 US IVD. Lyophilised Concentrated Monoclonal Antibody. F, P. MSDS. ... CD63-S. 0.1ml NCL-CD63. Lyophilised Concentrated Monoclonal Antibody. F, P. Info. MSDS. ...
CD63 antigen (CD63). Links to databases. *Uniprot P08962. Functions as cell surface receptor for TIMP1 and plays a role in the ... These curves are generated during the assay validation process using recombinant antigens. Please note that when analyzing ...
The role of tetraspanin CD63 in antigen presentation via MHC class II. Eur. J. Immunol. 41 (9), 2556-2561. doi:10.1002/eji. ... 2015). Tetraspanin CD63 acts as a pro-metastatic factorviaβ-catenin stabilization. Int. J. Cancer 136 (10), 2304-2315. doi: ... Yamazaki, S., Inaba, K., Tarbell, K. V., and Steinman, R. M. (2006). Dendritic cells expand antigen-specific Foxp3+CD25+CD4+ ... Gordon, H., Brockes, J. P., and Wilson, F. (1988). Appearance and regulation of an antigen associated with limb regeneration ...
  • CD63 is a 53 kD type III lysosomal glycoprotein also known as LIMP, LAMP-3, gp55, and melanoma-associated antigen (ME491). (biolegend.com)
  • This molecule is also referred to in the literature as LIMP, gp55, melanoma-associated antigen ME491, Pltgp40, LAMP-3 and is a member of the tetraspan transmembrane 4 superfamily (TM4SF). (fishersci.com)
  • 1992). "Genomic structure of the ME491/CD63 antigen gene and functional analysis of the 5'-flanking regulatory sequences. (thefullwiki.org)
  • Transcriptional enhancement of the human gene encoding for a melanoma-associated antigen (ME491) in association with malignant transformation. (thefullwiki.org)
  • Clone REA444 recognizes the rat CD63 antigen, a multi-pass membrane protein which is also known as mast cell antigen AD1 or melanoma antigen ME491. (miltenyibiotec.com)
  • 1992) The rat mast cell antigen AD1 (homologue to human CD63 or melanoma antigen ME491) is expressed in other cells in culture. (miltenyibiotec.com)
  • CD63 has been found to be identical to the ME491 antigen expressed by melanoma cells. (mybeckman.pl)
  • Use exosomes to deliver protein, RNA, DNA, or small molecule cargo to antigen-presenting cells with XStamp-CD40L. (systembio.com)
  • We constructed a bispecific antibody that is composed of a rapidly internalizing antibody binding to a tumor-associated antigen, ephrin receptor A2 (EphA2), and a noninternalizing antibody binding to a highly expressed tumor-associated antigen, activated leukocyte cell adhesion molecule (ALCAM). (aacrjournals.org)
  • We developed a bispecific antibody targeting ephrin receptor A2 (EphA2), a rapidly internalizing antigen, and activated leukocyte cell adhesion molecule (ALCAM), a non- or slowly internalizing antigen, and found that the bispecific becomes internalized when the ratio of EphA2 to ALCAM is greater than approximately 1:5. (aacrjournals.org)
  • Today, the HLDA Workshop meeting has been held 10 times and has over 371 CD antigens molecule have been identified. (sinobiological.com)
  • CD63 has been shown to associate with CD9, CD81, VLA-3, and VLA-6. (biolegend.com)
  • CD63 associates with transmembrane 4 superfamily members, CD9 and CD81, and with beta 1 integrins in human melanoma. (thefullwiki.org)
  • CD63 antigen associates non-covalently with CD9, CD81 and the integrins VLA-3, VLA-4 and VLA-6. (leicabiosystems.com)
  • Here, we reveal the presence of tetraspanin-enriched microdomains (TEMs) containing the tetraspanins CD9, CD63, CD81, and CD82 at the plasma membrane. (rupress.org)
  • The vesicles derived from mammalian cells contain a family of integral membrane proteins that cross four times the lipid bilayer and are called tetraspanins [ 11 ], including the surface markers of lymphocytes and antigen-presenting cells such as CD37, CD9, CD53, CD63, CD81, and CD82. (hindawi.com)
  • Some members of the tetraspanin family, such as CD63 , CD81, and CD9, are enriched in exosomes and have been recognized as exosome markers [37]. (thefreedictionary.com)
  • CD63, as other tetraspanins (CD9, CD81, CD82), has recently been reported as forming complexes with VLA-3 and phosphatidylinositol 4-kinase, with VLA-6, CD11/CD18 and tyrosine kinase. (mybeckman.pl)
  • We have so far established high quality monoclonal antibodies against human CD9, CD63 and CD81, which are members of tetraspanin superfamily and utilized as exosomal surface markers. (cosmobio.co.jp)
  • Immunofluorescence staining with 2C6 showed the lysosomes, and also elongated structures identified as Weibel-Palade bodies by their shape, distribution, and positive staining with anti-vWF antibodies, CD63 was also found by Western blotting of subcellular fractions highly enriched in Weibel-Palade bodies. (bloodjournal.org)
  • As the emitted fluorescence intensity is proportional to the binding sites of each single cell, the intensity will increase according to the number of FITC- conjugated antibodies bound to CD63 expressing cells. (wikipedia.org)
  • After degranulation a CD63 marker (labeled antibodies) is added to the test tube. (wikipedia.org)
  • The CD63 antibodies inhibit adhesion of neutrophils to activated endothelium. (mybeckman.pl)
  • Recently, we described a panel of monoclonal antibodies with superior selectivity for mesenchymal stem cells, including the monoclonal antibodies W8B2 against human mesenchymal stem cell antigen-1 (MSCA-1) and 39D5 against a CD56 epitope, which is not expressed on natural killer cells. (haematologica.org)
  • Design and Methods Bone marrow derived mesenchymal stem cells from healthy donors were analyzed and isolated by flow cytometry using a large panel of antibodies against surface antigens including CD271, MSCA-1, and CD56. (haematologica.org)
  • A modification using precooled absolute methanol has been shown to give better results for certain cell cycle antigens such as Ki-67 and PCNA when using FITC-conjugated antibodies. (thermofisher.com)
  • When the EphA2-to-ALCAM ratio is greater than a threshold level (1:5), the amount of the bispecific taken into the tumor cell exceeds what is achieved by either the monoclonal internalizing antibody or a mixture of the two antibodies, showing a bispecific-dependent amplification effect where a small amount of the internalizing antigen EphA2 induces internalization of a larger amount of the noninternalizing antigen ALCAM. (aacrjournals.org)
  • Immunohistochemical staining was performed using a sensitive labelled streptavidin biotin (LSAB kit, Dako) on tumours using monoclonal antibodies for tetraspanin KAI1 (C33, Novacastra) and for proliferation index Ki-67 (Clone MIB-1, Dako, Denmark) after antigen retrieval. (bmj.com)
  • Interrupting macrophage presentation of donor antigens may be a solution to prevent generation of these destructive antibodies and the ensuing chronic rejection. (sciencemag.org)
  • De novo donor-specific antibodies (DSAs) to mismatched donor human leukocyte antigen after human lung transplantation predispose lung grafts to chronic rejection. (sciencemag.org)
  • Moreover, the combination of these antibodies with one against a cancer cell surface antigen resulted in sensitive detection of cancer cell derived exosomes. (cosmobio.co.jp)
  • CD antigens for cluster of differentiation, which indicates a defined subset of cellular surface receptors (epitopes) that identify cell type and stage of differentiation, and which are recognized by antibodies. (sinobiological.com)
  • 1993). "The protein CD63 is in platelet dense granules, is deficient in a patient with Hermansky-Pudlak syndrome, and appears identical to granulophysin. (thefullwiki.org)
  • CD63 was later identified as granulophysin, a platelet dense granule glycoprotein. (mybeckman.pl)
  • The H5C6 monoclonal antibody specifically binds to CD63. (fishersci.com)
  • There are currently no images for CD63 Antibody (NBP2-47936JF549). (novusbio.com)
  • One antibody, 2C6, was found to be specific for CD63, a membrane glycoprotein previously described in the lysosomes of platelets and other cell types. (bloodjournal.org)
  • The immunopurified 2C6 antigen was recognized by an anti-CD63 reference antibody, 2.28, by Western blotting. (bloodjournal.org)
  • CD63 antibody LS-C700468 is a Cy3-conjugated rabbit polyclonal antibody to CD63 (aa103-203) from human. (lsbio.com)
  • The antibody MEM-259 reacts with an extracellular/luminal epitope of CD63 (LAMP-3), a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells. (exbio.cz)
  • Each antibody is crafted with care according to rigorous protocols for immunogen design and preparation, presentation to host animal, and high-affinity purification against the antigen. (abgent.com)
  • CLBGran/12 was used as a CD63 reference monoclonal antibody during HLDA 6. (mybeckman.pl)
  • In another example, a bispecific composed of a moderately internalizing antibody arm (anti-HER2) and an internalization-inducing antibody arm (anti-CD63, anti-PRLR, or anti-APLP2) was constructed and used to improve ADC uptake ( 5-7 ). (aacrjournals.org)
  • Additionally, in an allogeneic cell transfer protocol, antigen presentation by AMs was Zbtb7a-dependent where AMs deficient in Zbtb7a failed to induce antibody and T cell responses. (sciencemag.org)
  • Mouse monoclonal antibody raised against native CD63. (abnova.com)
  • See 9 reference sequence protein isoforms for the CD63 gene. (nih.gov)
  • CD63 is a member of the tetraspan transmembrane superfamily (TM4SF) protein expressed on activated platelets, monocytes/macrophages, endothelium, fibroblasts, osteoclasts, and smooth muscle cells. (biolegend.com)
  • CD63 may be involved in platelet activation and is thought to function as a transmembrane adaptor protein. (biolegend.com)
  • CD63 antigen is a protein that, in humans, is encoded by the CD63 gene. (wikipedia.org)
  • This MAb recognizes protein of 26kDa-60kDa, which is identified as CD63. (novusbio.com)
  • Purified recombinant protein of full-length mouse Cd63 antigen (cDNA clone MGC:107286 IMAGE:30284264), complete cds, with C-terminal MYC/DDK tag, was expressed in HEK293T cells. (creativebiomart.net)
  • EVs derived from antigen presenting cells (APCs) that are loaded with either peptide or whole protein antigens are reported to induce anti-tumor immunity in animal models but show only modest improvements in cancer patients ( 2 , 7 - 9 ). (frontiersin.org)
  • In addition, western blot analysis demonstrated that the protein level of bone marrow stromal antigen‑2 (BST‑2) was downregulated by TGN. (spandidos-publications.com)
  • Bone marrow stromal antigen 2 (BST-2), also referred to as CD317, tetherin and HM1.24 is a multifunctional protein. (spandidos-publications.com)
  • The CD63 marker is a fluorescein isothiocyanate labeled antigen which can bind to an CD63 protein and is used to sort the cells via FACS(Fluorescence activated cell sorting/sorter). (wikipedia.org)
  • The protein encoded by CD63 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. (antibodies-online.cn)
  • The Muted protein is important for the steady-state level of CD63 . (antibodies-online.cn)
  • Silencing of HRS , STAM1 or TSG101 reduced the secretion of EV-associated CD63 and MHC II but each gene altered differently the size and/or protein composition of secreted EVs, as quantified by immuno-electron microscopy. (biologists.org)
  • CD63 is mainly associated with membranes of intracellular vesicles, although cell surface expression may be induced. (wikipedia.org)
  • CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. (novusbio.com)
  • A novel lysosomal membrane glycoprotein, cloned by a screening procedure for intracellular antigens in eukaryotic cells. (thefullwiki.org)
  • 2001) Dynamics of intracellular granules with CD63-GFP in rat basophilic leukemia cells. (miltenyibiotec.com)
  • The FIX & PERM Cell Permeabilization Kit consists of matched Fixation Reagent (Medium A) and Permeabilization Reagent (Medium B) for simultaneous analysis of intracellular and cell-surface antigens in the same cell population (Figure 1) . (thermofisher.com)
  • Use of FIX & PERM Cell Permeabilization Kit for simultaneous surface antigen and intracellular antigen staining. (thermofisher.com)
  • This standard procedure for intracellular staining with FIX & PERM fixation & permeabilization reagents gives optimal results for most antigens. (thermofisher.com)
  • cGMP-phosphodiesterase 6 can be activated by another cancer-retina antigen, transducin, through Wnt5a-Frizzled-2 cascade, which leads to a lowering of cGMP and an increase in intracellular calcium mobilization. (cusabio.com)
  • In macrophages, HIV-1 has been shown to bud into intracellular structures that contain the late endosome marker CD63. (pubmedcentralcanada.ca)
  • On uninfected macrophages, these proteins are seen at the cell surface and in intracellular vacuole-like structures with a complex content of vesicles and interconnected membranes that lack endosome markers, including CD63. (pubmedcentralcanada.ca)
  • Thrombin-activated human peripheral blood platelets were stained with CD63 (clone H5C6) Alexa Fluor® 700 (filled histogram) or mouse IgG1, κ Alexa Fluor® 700 isotype control (open histogram). (biolegend.com)
  • Clone REA563 recogizes the mouse CD63 antigen. (miltenyibiotec.com)
  • Interaction of CD63 with other membrane proteins or adaptors regulates cell activities such as adhesion, migration, and degranulation. (miltenyibiotec.com)
  • CD63 belongs to the tetraspanin transmembrane 4 superfamily (TM4SF) of proteins, which are characterized by four membrane spanning domains. (miltenyibiotec.com)
  • Our results indicate that CD63 colocalizes with vWF and P- selectin in the Weibel-Palade bodies of endothelial cells, and together with these adhesion proteins it could be rapidly expressed on the cell surface in areas of vascular injury and inflammation. (bloodjournal.org)
  • The role of DCs in antigens presentation of heated milk proteins will be addressed. (wur.nl)
  • Several minutes at room temperature gives the marker time to bind to the CD63 proteins on the cell membrane of the basophil. (wikipedia.org)
  • CD63 has been shown to interact with CD117 and CD82. (wikipedia.org)
  • Comments regarding 'Marked improvement of the basophil activation test by detecting CD203c instead of CD63' by Boumiza et al. (nih.gov)
  • In addition, changes in the expression of basophil surface's molecules are observed: some antigens appear de novo (CD63, CD107a), and other increase their expression (CD13, CD203c). (termedia.pl)
  • The CD203c antigen is one of the most important marker of basophil's activation. (termedia.pl)
  • The production of IL-35, TNF-α, TGF-ß, IL-10, CD63, CD203c and some other cytokines will be tested by ELISA, Cytometric Bead Array and Flow Cytometry. (wur.nl)
  • BAT for wheat was performed using two flow cytometric procedures based, respectively, on the expression of CD63 (Becton Dickinson-BD Biosciences, San Jose, CA) and CD203c (Beckman Coulter, Fullerton, CA) on the cell surface of basophils activated in vitro by exposure to wheat in double dilution. (thefreedictionary.com)
  • CD63 functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. (miltenyibiotec.com)
  • iiii) TIMP-1, through receptor ( CD63 ) binding, has a role in multiple biological processes, including inflammation and immune regulation. (thefreedictionary.com)
  • CD63 is a good marker for flow cytometric quantification of in vitro activated basophils for diagnosis of IgE-mediated allergy. (wikipedia.org)
  • In cell biology, CD63 is often used as a marker for multivesicular bodies, which in some cells are enriched with CD63, as well as for extracellular vesicles released from either the multivesicular body or the plasma membrane. (wikipedia.org)
  • Analysis of membrane expression of the CD63 human basophil activation marker. (nih.gov)
  • Western blotting was used to assess the exosome biomarkers CD63 and flotillin as well as the negative control marker calreticulin (Figure 1B). (thefreedictionary.com)
  • As flow cytometry is a valuable tool for analyzing large numbers of cells and for identifying cell populations, even at low concentrations, the percentage of basophils activated after in vitro stimulation by allergens and expressing the CD63 marker can be determined. (wikipedia.org)
  • Studies have shown that CD63 may be a marker for granule release. (mybeckman.pl)
  • CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. (novusbio.com)
  • They should express specific cell surface markers, such as cluster of differentiation (CD) 73, D90, CD105, and lack the expression of CD14, CD34, CD45 and human leukocyte antigen-DR (HLA-DR). (news-medical.net)
  • The CD antigens / Cluster of differentiation nomenclature was established in the 1st International Workshop and Conference on Human Leukocyte Differentiation Antigens (HLDA), which was held in Paris in 1982. (sinobiological.com)
  • Although IHC is not commonly necessary in differentiation, epithelial membrane antigen (EMA) highlights most mature sebocytes in sebaceoma, whereas its expression is uncommon in BCC. (thefreelibrary.com)
  • Altered expression of CD63 and exosomes in scleroderma dermal fibroblasts. (nih.gov)
  • Target exosomes to antigen-presenting cells with the pre-built XStamp-CD40L Lentivector Construct. (systembio.com)
  • Exosomes made from cells transfected or transduced with the XStamp-CD40L Lentivector will now be targeted to cells expressing CD40, primarily antigen-presenting cells. (systembio.com)
  • Here we show that inactivating the vacuolar ATPase in HeLa cells causes a dramatic increase in the production of exosomes, which display endocytosed tracers, cholesterol, and CD63. (bris.ac.uk)
  • CD63 is a 53 kDa, type III lysosomal glycoprotein, expressed on activated platelets, monocytes and macrophages. (fishersci.com)
  • The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. (novusbio.com)
  • The CD63 antigen, also known as lysosomal membrane associated glycoprotein 3 (LAMP3), is a member of the tetraspanin (TM4SF) family. (mybeckman.pl)
  • CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. (novusbio.com)
  • See the genomic reference sequence for the CD63 gene (NG_008347.1). (nih.gov)
  • See reference mRNA sequence for the CD63 gene (XM_024449283.1). (nih.gov)
  • It is reported that CD63 antigen may play a role as a tumor suppressor gene, as its expression in human melanoma cells reduces tumor spread and metastasis. (leicabiosystems.com)
  • We report here the use of gene expression profiling to identify BAX-δ as a novel candidate tumor antigen in ALL. (aacrjournals.org)
  • A lack of expression of hematopoietic antigens (CD45, CD34, CD14/CD11b, CD79a/CD19, HLA-DR) is recommended, along with a minimum purity of ≥95% for CD105, CD73, and CD90 positive cells and ≤2% expression of hematopoietic antigens. (news-medical.net)
  • Analysis of the cellular molecules present in the fractions containing virus or exclusively cellular material demonstrated that virus and cellular vesicles share several cellular antigens, with the exception of CD43 and CD63, found mainly at the virus surface, and HLA-DQ, which was found only in the cellular vesicles. (nih.gov)
  • Microvesicles are small vesicles expressing specific antigens from their cells of origin. (biomedcentral.com)
  • Such differences could be attributed to a greater heterogeneity in size, and higher MHC II and lower CD63 levels in vesicles recovered from DCs as compared with HeLa-CIITA. (biologists.org)
  • Also, the biosynthetic profile of the 2C6 antigen in endothelial cells showed a nascent molecular mass and a glycosylation pattern identical to that of CD63. (bloodjournal.org)
  • reported elevated total Annexin V EV, platelet EV ( CD63 , P-selectin-exposing), and endothelial EV (CD62, E-selectin-exposing) in 27 young healthy women compared to men while at rest. (thefreedictionary.com)
  • CD63 antigen is a member of the TM4 superfamily with its structure consisting of four transmembrane regions, short cytoplasmic N and C-termini and two extracellular regions. (leicabiosystems.com)
  • Mantegazza AR, Barrio MM, Moutel S, Bover L, Weck M, Brossart P, Teillaud JL, Mordoh J: CD63 tetraspanin slows down cell migration and translocates to the endosomal-lysosomal-MIICs route after extracellular stimuli in human immature dendritic cells. (exbio.cz)
  • Anaphylactic degranulation results in the fusion of the main histamine-containing granules with the plasma membrane, releasing the entire contents of granules to the extracellular space and exposing CD63 on the surface of basophils [120]. (thefreedictionary.com)
  • Attenuation correlated with increased oxidants in macrophages in response to DKO infection and enhanced labeling of lysosomal markers (CD63 and rab7) on DKO phagosomes. (jove.com)
  • Expression of DSA-induced Zbtb7a was restricted to alveolar macrophages (AMs), and selective disruption of Zbtb7a in AMs resulted in less bronchiolar occlusion, low immune responses to lung-restricted self-antigens, and high protection from chronic rejection in mice. (sciencemag.org)
  • Human CD63 is expressed on activated platelets and also on monocytes and macrophages. (biomol.com)
  • It is an in vitro method where a wide variety of stimuli can be tested, incubating them with the patient's blood sample, and observing basophil activation (indication of hypersensitivity) through upregulation of CD63 (or other basophil activation markers) on this leucocyte's membrane. (usp.br)
  • A new variant of the basophil activation test for allergen-induced basophil CD63 upregulation. (nih.gov)
  • Degranulated cell expose CD63 molecules on their outer cell membrane, hence the granules, which contain CD63 molecules on their inner surface, merged with the cell membrane. (wikipedia.org)
  • The CD antigens are protocol used for the identification and investigation of cell surface molecules providing targets for immunophenotyping of cells. (sinobiological.com)
  • CD63 is located on the basophilic granule membranes in resting basophils, mast cells, and platelets, and is also located on the plasma membranes of these cells. (miltenyibiotec.com)
  • Although inhalation or ingestion of allergens activates mucosal mast cells, i.v. or s.c. antigen entry activates circulating basophils and connective tissue mast cells. (rupress.org)
  • Moreover, primary B-ALL cells were recognized by BAX-δ-specific CTL, indicating that this antigen is naturally processed and presented by tumor cells. (aacrjournals.org)
  • TIMP1 (显示 TIMP1 ELISA试剂盒 ) signaling via CD63 leads to activation of hepatic stellate cells, which create an environment in the liver that increases its susceptibility to pancreatic tumor cells. (antibodies-online.cn)
  • This FITC labeled antigen emits light at a wavelength of 530 nm. (wikipedia.org)
  • Genetic localization of Cd63, a member of the transmembrane 4 superfamily, reveals two distinct loci in the mouse genome. (thefullwiki.org)
  • Exosomal markers CD63 and CD9 (显示 CD9 ELISA试剂盒 ) are elevated in pancreatic tumor tissues. (antibodies-online.cn)
  • Recently, the characterization and understanding of their biological role have become a main research area due to their potential role in vaccination, as biomarkers antigens, early diagnostic tools, and therapeutic applications. (hindawi.com)
  • Therefore, EVs are potential biomarkers and antigens for vaccination, with potential uses for early diagnostic, and therapeutic applications in several diseases. (hindawi.com)
  • The identification of new tumor-associated antigens (TAA) is critical for the development of effective immunotherapeutic strategies, particularly in diseases like B-cell acute lymphoblastic leukemia (B-ALL), where few target epitopes are known. (aacrjournals.org)
  • Several studies have shown that T-cell responses specific for distinct tumor antigens can be primed or expanded in the cancer-bearing host ( 1 - 4 ), suggesting that the immune response against tumors can be manipulated in humans for therapeutic benefit. (aacrjournals.org)
  • Ideally, these antigens should be preferentially or uniquely expressed by malignant cells, play essential roles on tumor cell survival or proliferation, or be broadly expressed on tumors, and their reactive T-cell repertoire should be spared from negative selection or functional inactivation ( 13 ). (aacrjournals.org)
  • This set of genes would fulfill an essential requirement of tumor antigens (i.e., their overexpression in cancer cells compared with normal tissues) and serve as a pool of target antigens from which to identify those capable of eliciting a tumor-specific T-cell response. (aacrjournals.org)
  • Cell surface exposition of CD63 is usually activation-dependent. (exbio.cz)
  • Tissue section of human prostate containing adenocarcinoma that has been immunostained for the cell-surface antigen CD63. (cellimagelibrary.org)
  • This image is part of a large collection of immunohistochemistry images of cell-surface antigens generated by the SCGAP Urologic Epithelial Stem Cells (UESC) Project. (cellimagelibrary.org)
  • They have been implicated in various functions in both health and disease, including intercellular communication, antigen presentation, prion transmission, and tumour cell metastasis. (bris.ac.uk)
  • Thus, the internalizing property of a cell surface antigen can be manipulated in either direction by a neighboring antigen, and this phenomenon can be exploited for therapeutic targeting. (aacrjournals.org)
  • The key question is whether the internalization propensity of a given cell surface antigen can be impacted by its neighboring surface antigens, and if so what are the parameters that govern the conversion of a noninternalizing antigen to an internalizing antigen, and vice versa. (aacrjournals.org)
  • Annexin V - positive microvesicles, leukocyte (CD45-positive), platelet (CD61-positive), activated platelet (CD62P-, CD63-positive), endothelium-derived (CD62E-positive) and tissue-factor (CD142-positive) microvesicles were identified in the peripheral blood of patients with soft tissue sarcoma ( n = 39) and healthy controls ( n = 17) using fluorescence-activated cell sorting (FACS). (biomedcentral.com)
  • Data show that CD63 is a crucial player in the regulation of the tumor cell-intrinsic metastatic potential by affecting cell plasticity. (antibodies-online.cn)
  • As a siganl, CD antigens usually initiated, altering the behavior of the cell. (sinobiological.com)
  • Non peptide antigen presentation to T-cell receptors on NKT cells. (sinobiological.com)
  • CD63 appears to be involved with cell adhesion and associates with VLA-3 and VLA-6 integrins. (biomol.com)
  • CD63 antigen is widely distributed on the surface and interior of both hematopoietic and non-hematopoietic cells such as most sweat glands, islets of Langerhans, pituitary, pancreas, peribronchial glands, Paneth cells and prostate glands. (leicabiosystems.com)
  • CD63 was first described in granules of resting platelets and on the surface membrane on activated platelets. (mybeckman.pl)
  • CD63 antigen, present in azurophilic granules of non-stimulated neutrophils, is strongly expressed on the surface of neutrophils after activation. (mybeckman.pl)
  • 2) MSCs must express the surface antigens CD105, CD73, and CD90. (news-medical.net)
  • We found that the overall internalization property of the bispecific is profoundly impacted by the relative surface expression level (antigen density ratio) of EphA2 versus ALCAM. (aacrjournals.org)
  • Development of the sensitive quantification method for exosome using their surface antigens. (cosmobio.co.jp)
  • Flow cytometry analysis of IgE-activated peripheral blood stained with anti-human CD63 (MEM-259) purified, GAM-APC. (exbio.cz)
  • Flow cytometry analysis of IgE-activated peripheral blood showing CD34 positive cells (blue) stained with anti-human CD63 (MEM-259) PE-Cy™5. (exbio.cz)
  • This image is part of a large collection of images generated from numerous specimens to characterize the distribution of CD63 in human prostate tissue. (cellimagelibrary.org)
  • Links are provided below for the UESC Project database, the entire human prostate immunostain summary, the CD63 immunostain summary, and information on the specimen that this image is from. (cellimagelibrary.org)
  • Other images of CD63 human prostate immunostains are accessible following the group link. (cellimagelibrary.org)
  • In human dendritic cells (DCs), ALIX depletion also increased MHC II in the cells, but not in the released CD63-positive EVs. (biologists.org)
  • Interactions with CD63 may therefore affect the trafficking and function of β2 integrins. (wikipedia.org)
  • Specific association of CD63 with the VLA-3 and VLA-6 integrins. (thefullwiki.org)
  • CD63 is a critical player in LMP1 (显示 PDLIM7 ELISA试剂盒 ) exosomal trafficking and LMP1 (显示 PDLIM7 ELISA试剂盒 )-mediated enhancement of exosome production and may play further roles in limiting downstream LMP1 (显示 PDLIM7 ELISA试剂盒 ) signaling. (antibodies-online.cn)
  • An in vitro Antigen 85B peptide presentation assay was used to determine antigen presentation to T cells by APCs infected with DKO or other mycobacterial strains. (jove.com)