Musculoskeletal Diseases: Diseases of the muscles and their associated ligaments and other connective tissue and of the bones and cartilage viewed collectively.Musculoskeletal System: The MUSCLES, bones (BONE AND BONES), and CARTILAGE of the body.Musculoskeletal Pain: Discomfort stemming from muscles, LIGAMENTS, tendons, and bones.Hydroxymethylglutaryl-CoA Reductase Inhibitors: Compounds that inhibit HMG-CoA reductases. They have been shown to directly lower cholesterol synthesis.Home Care Agencies: Public or private organizations that provide, either directly or through arrangements with other organizations, home health services in the patient's home. (Hospital Administration Terminology, 2d ed)Rheumatic Diseases: Disorders of connective tissue, especially the joints and related structures, characterized by inflammation, degeneration, or metabolic derangement.Military Personnel: Persons including soldiers involved with the armed forces.Hospitals, Military: Hospitals which provide care for the military personnel and usually for their dependents.Joint DiseasesMilitary Medicine: The practice of medicine as applied to special circumstances associated with military operations.Lymphocyte Function-Associated Antigen-1: An integrin heterodimer widely expressed on cells of hematopoietic origin. CD11A ANTIGEN comprises the alpha chain and the CD18 antigen (ANTIGENS, CD18) the beta chain. Lymphocyte function-associated antigen-1 is a major receptor of T-CELLS; B-CELLS; and GRANULOCYTES. It mediates the leukocyte adhesion reactions underlying cytolytic conjugate formation, helper T-cell interactions, and antibody-dependent killing by NATURAL KILLER CELLS and granulocytes. Intracellular adhesion molecule-1 has been defined as a ligand for lymphocyte function-associated antigen-1.Fluorescein-5-isothiocyanate: Fluorescent probe capable of being conjugated to tissue and proteins. It is used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.Histocompatibility: The degree of antigenic similarity between the tissues of different individuals, which determines the acceptance or rejection of allografts.Antigens, CD58: Glycoproteins with a wide distribution on hematopoietic and non-hematopoietic cells and strongly expressed on macrophages. CD58 mediates cell adhesion by binding to CD2; (ANTIGENS, CD2); and this enhances antigen-specific T-cell activation.Thiocyanates: Organic derivatives of thiocyanic acid which contain the general formula R-SCN.Fluoresceins: A family of spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one derivatives. These are used as dyes, as indicators for various metals, and as fluorescent labels in immunoassays.Fluorescent Dyes: Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.Dextrans: A group of glucose polymers made by certain bacteria. Dextrans are used therapeutically as plasma volume expanders and anticoagulants. They are also commonly used in biological experimentation and in industry for a wide variety of purposes.Intercellular Adhesion Molecule-1: A cell-surface ligand involved in leukocyte adhesion and inflammation. Its production is induced by gamma-interferon and it is required for neutrophil migration into inflamed tissue.Antigens, CD2: Glycoprotein members of the immunoglobulin superfamily which participate in T-cell adhesion and activation. They are expressed on most peripheral T-lymphocytes, natural killer cells, and thymocytes, and function as co-receptors or accessory molecules in the T-cell receptor complex.Membrane Microdomains: Detergent-insoluble CELL MEMBRANE components. They are enriched in SPHINGOLIPIDS and CHOLESTEROL and clustered with glycosyl-phosphatidylinositol (GPI)-anchored proteins.Receptors, Antigen, T-Cell: Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.beta-Cyclodextrins: Cyclic GLUCANS consisting of seven (7) glucopyranose units linked by 1,4-glycosidic bonds.Adaptor Proteins, Signal Transducing: A broad category of carrier proteins that play a role in SIGNAL TRANSDUCTION. They generally contain several modular domains, each of which having its own binding activity, and act by forming complexes with other intracellular-signaling molecules. Signal-transducing adaptor proteins lack enzyme activity, however their activity can be modulated by other signal-transducing enzymesSignal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Antigen-Presenting Cells: A heterogeneous group of immunocompetent cells that mediate the cellular immune response by processing and presenting antigens to the T-cells. Traditional antigen-presenting cells include MACROPHAGES; DENDRITIC CELLS; LANGERHANS CELLS; and B-LYMPHOCYTES. FOLLICULAR DENDRITIC CELLS are not traditional antigen-presenting cells, but because they hold antigen on their cell surface in the form of IMMUNE COMPLEXES for B-cell recognition they are considered so by some authors.Jurkat Cells: A CELL LINE derived from human T-CELL LEUKEMIA and used to determine the mechanism of differential susceptibility to anti-cancer drugs and radiation.Caveolae: Endocytic/exocytic CELL MEMBRANE STRUCTURES rich in glycosphingolipids, cholesterol, and lipid-anchored membrane proteins that function in ENDOCYTOSIS (potocytosis), transcytosis, and SIGNAL TRANSDUCTION. Caveolae assume various shapes from open pits to closed vesicles. Caveolar coats are composed of CAVEOLINS.CD4-Positive T-Lymphocytes: A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.Alopecia Areata: Loss of scalp and body hair involving microscopically inflammatory patchy areas.Alopecia: Absence of hair from areas where it is normally present.Scalp: The outer covering of the calvaria. It is composed of several layers: SKIN; subcutaneous connective tissue; the occipitofrontal muscle which includes the tendinous galea aponeurotica; loose connective tissue; and the pericranium (the PERIOSTEUM of the SKULL).Hair Follicle: A tube-like invagination of the EPIDERMIS from which the hair shaft develops and into which SEBACEOUS GLANDS open. The hair follicle is lined by a cellular inner and outer root sheath of epidermal origin and is invested with a fibrous sheath derived from the dermis. (Stedman, 26th ed) Follicles of very long hairs extend into the subcutaneous layer of tissue under the SKIN.Judaism: The religion of the Jews characterized by belief in one God and in the mission of the Jews to teach the Fatherhood of God as revealed in the Hebrew Scriptures. (Webster, 3d ed)Hair: A filament-like structure consisting of a shaft which projects to the surface of the SKIN from a root which is softer than the shaft and lodges in the cavity of a HAIR FOLLICLE. It is found on most surfaces of the body.T-Lymphocytes: Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.Autoimmune Diseases: Disorders that are characterized by the production of antibodies that react with host tissues or immune effector cells that are autoreactive to endogenous peptides.Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Antigens, CD20: Unglycosylated phosphoproteins expressed only on B-cells. They are regulators of transmembrane Ca2+ conductance and thought to play a role in B-cell activation and proliferation.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Antibody Specificity: The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.Antibodies, Monoclonal, Murine-Derived: Antibodies obtained from a single clone of cells grown in mice or rats.Antibodies, Viral: Immunoglobulins produced in response to VIRAL ANTIGENS.Antibodies, Bacterial: Immunoglobulins produced in a response to BACTERIAL ANTIGENS.Antibodies, Neutralizing: Antibodies that reduce or abolish some biological activity of a soluble antigen or infectious agent, usually a virus.Antibody Formation: The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.Epitopes: Sites on an antigen that interact with specific antibodies.Propylene Glycol: A clear, colorless, viscous organic solvent and diluent used in pharmaceutical preparations.Firefly Luciferin: A benzothaizole which is oxidized by LUCIFERASES, FIREFLY to cause emission of light (LUMINESCENCE).Alkynes: Hydrocarbons with at least one triple bond in the linear portion, of the general formula Cn-H2n-2.Oximes: Compounds that contain the radical R2C=N.OH derived from condensation of ALDEHYDES or KETONES with HYDROXYLAMINE. Members of this group are CHOLINESTERASE REACTIVATORS.Aequorin: A photoprotein isolated from the bioluminescent jellyfish Aequorea. It emits visible light by an intramolecular reaction when a trace amount of calcium ion is added. The light-emitting moiety in the bioluminescence reaction is believed to be 2-amino-3-benzyl-5-(p-hydroxyphenyl)pyrazine (AF-350).Methanol: A colorless, flammable liquid used in the manufacture of FORMALDEHYDE and ACETIC ACID, in chemical synthesis, antifreeze, and as a solvent. Ingestion of methanol is toxic and may cause blindness.Propylene Glycols: Derivatives of propylene glycol (1,2-propanediol). They are used as humectants and solvents in pharmaceutical preparations.Ethanol: A clear, colorless liquid rapidly absorbed from the gastrointestinal tract and distributed throughout the body. It has bactericidal activity and is used often as a topical disinfectant. It is widely used as a solvent and preservative in pharmaceutical preparations as well as serving as the primary ingredient in ALCOHOLIC BEVERAGES.Tyramine: An indirect sympathomimetic. Tyramine does not directly activate adrenergic receptors, but it can serve as a substrate for adrenergic uptake systems and monoamine oxidase so it prolongs the actions of adrenergic transmitters. It also provokes transmitter release from adrenergic terminals. Tyramine may be a neurotransmitter in some invertebrate nervous systems.Luminescent Measurements: Techniques used for determining the values of photometric parameters of light resulting from LUMINESCENCE.Atlantic Islands: Widely scattered islands in the Atlantic Ocean as far north as the AZORES and as far south as the South Sandwich Islands, with the greatest concentration found in the CARIBBEAN REGION. They include Annobon Island, Ascension, Canary Islands, Falkland Islands, Fernando Po (also called Isla de Bioko and Bioko), Gough Island, Madeira, Sao Tome and Principe, Saint Helena, and Tristan da Cunha.Sexology: This discipline concerns the study of SEXUALITY, and the application of sexual knowledge such as sexual attitudes, psychology, and SEXUAL BEHAVIOR. Scope of application generally includes educational (SEX EDUCATION), clinical (SEX COUNSELING), and other settings.Parapsychology: Branch of psychology that deals with paranormal behavior and events such as telepathy, precognition, and clairvoyance, which are not explicable by present day "natural laws".Perciformes: The most diversified of all fish orders and the largest vertebrate order. It includes many of the commonly known fish such as porgies, croakers, sunfishes, dolphin fish, mackerels, TUNA, etc.Psychology, Experimental: The branch of psychology which seeks to learn more about the fundamental causes of behavior by studying various psychologic phenomena in controlled experimental situations.History, 20th Century: Time period from 1901 through 2000 of the common era.BerlinPsychoanalysis: The separation or resolution of the psyche into its constituent elements. The term has two separate meanings: 1. a procedure devised by Sigmund Freud, for investigating mental processes by means of free association, dream interpretation and interpretation of resistance and transference manifestations; and 2. a theory of psychology developed by Freud from his clinical experience with hysterical patients. (From Campbell, Psychiatric Dictionary, 1996).Occultism: The belief in or study of practices and knowledge of magical, mystical, or supernatural powers. ALCHEMY, astrology, and many RELIGIOUS PHILOSOPHIES are based upon occult principles.Silicic Acid: A hydrated form of silicon dioxide. It is commonly used in the manufacture of TOOTHPASTES and as a stationary phase for CHROMATOGRAPHY.North CarolinaInvestigational New Drug Application: An application that must be submitted to a regulatory agency (the FDA in the United States) before a drug can be studied in humans. This application includes results of previous experiments; how, where, and by whom the new studies will be conducted; the chemical structure of the compound; how it is thought to work in the body; any toxic effects found in animal studies; and how the compound is manufactured. (From the "New Medicines in Development" Series produced by the Pharmaceutical Manufacturers Association and published irregularly.)Drugs, Investigational: Drugs which have received FDA approval for human testing but have yet to be approved for commercial marketing. This includes drugs used for treatment while they still are undergoing clinical trials (Treatment IND). The main heading includes drugs under investigation in foreign countries.Carcinoembryonic Antigen: A glycoprotein that is secreted into the luminal surface of the epithelia in the gastrointestinal tract. It is found in the feces and pancreaticobiliary secretions and is used to monitor the response to colon cancer treatment.Immunosuppressive Agents: Agents that suppress immune function by one of several mechanisms of action. Classical cytotoxic immunosuppressants act by inhibiting DNA synthesis. Others may act through activation of T-CELLS or by inhibiting the activation of HELPER CELLS. While immunosuppression has been brought about in the past primarily to prevent rejection of transplanted organs, new applications involving mediation of the effects of INTERLEUKINS and other CYTOKINES are emerging.Inflammatory Bowel Diseases: Chronic, non-specific inflammation of the GASTROINTESTINAL TRACT. Etiology may be genetic or environmental. This term includes CROHN DISEASE and ULCERATIVE COLITIS.United States Food and Drug Administration: An agency of the PUBLIC HEALTH SERVICE concerned with the overall planning, promoting, and administering of programs pertaining to maintaining standards of quality of foods, drugs, therapeutic devices, etc.Academic Medical Centers: Medical complexes consisting of medical school, hospitals, clinics, libraries, administrative facilities, etc.Medical Oncology: A subspecialty of internal medicine concerned with the study of neoplasms.Ethics Committees, Research: Hospital or other institutional committees established to protect the welfare of research subjects. Federal regulations (the "Common Rule" (45 CFR 46)) mandate the use of these committees to monitor federally-funded biomedical and behavioral research involving human subjects.Herpesvirus 4, Human: The type species of LYMPHOCRYPTOVIRUS, subfamily GAMMAHERPESVIRINAE, infecting B-cells in humans. It is thought to be the causative agent of INFECTIOUS MONONUCLEOSIS and is strongly associated with oral hairy leukoplakia (LEUKOPLAKIA, HAIRY;), BURKITT LYMPHOMA; and other malignancies.Arthritis, Rheumatoid: A chronic systemic disease, primarily of the joints, marked by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Etiology is unknown, but autoimmune mechanisms have been implicated.Fibroblasts: Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.Epstein-Barr Virus Nuclear Antigens: Nuclear antigens encoded by VIRAL GENES found in HUMAN HERPESVIRUS 4. At least six nuclear antigens have been identified.Synovial Membrane: The inner membrane of a joint capsule surrounding a freely movable joint. It is loosely attached to the external fibrous capsule and secretes SYNOVIAL FLUID.Epstein-Barr Virus Infections: Infection with human herpesvirus 4 (HERPESVIRUS 4, HUMAN); which may facilitate the development of various lymphoproliferative disorders. These include BURKITT LYMPHOMA (African type), INFECTIOUS MONONUCLEOSIS, and oral hairy leukoplakia (LEUKOPLAKIA, HAIRY).Fibroblast Growth Factor 2: A single-chain polypeptide growth factor that plays a significant role in the process of WOUND HEALING and is a potent inducer of PHYSIOLOGIC ANGIOGENESIS. Several different forms of the human protein exist ranging from 18-24 kDa in size due to the use of alternative start sites within the fgf-2 gene. It has a 55 percent amino acid residue identity to FIBROBLAST GROWTH FACTOR 1 and has potent heparin-binding activity. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor (FIBROBLAST GROWTH FACTOR 1).Viral Matrix Proteins: Proteins associated with the inner surface of the lipid bilayer of the viral envelope. These proteins have been implicated in control of viral transcription and may possibly serve as the "glue" that binds the nucleocapsid to the appropriate membrane site during viral budding from the host cell.Descemet Stripping Endothelial Keratoplasty: A surgical procedure or KERATOPLASTY involving selective stripping and replacement of diseased host DESCEMET MEMBRANE and CORNEAL ENDOTHELIUM with a suitable and healthy donor posterior lamella. The advantage to this procedure is that the normal corneal surface of the recipient is retained, thereby avoiding corneal surface incisions and sutures.Cell Line: Established cell cultures that have the potential to propagate indefinitely.
Intracellular adhesion molecule-1 modulates beta-chemokines and directly costimulates T cells in vivo. (1/194)The potential roles of adhesion molecules in the expansion of T cell-mediated immune responses in the periphery were examined using DNA immunogen constructs as model antigens. We coimmunized cDNA expression cassettes encoding the adhesion molecules intracellular adhesion molecule-1 (ICAM-1), lymphocyte function associated-3 (LFA-3), and vascular cell adhesion molecule-1 (VCAM-1) along with DNA immunogens, and we analyzed the resulting antigen-specific immune responses. We observed that antigen-specific T-cell responses can be enhanced by the coexpression of DNA immunogen and adhesion molecules ICAM-1 and LFA-3. Coexpression of ICAM-1 or LFA-3 molecules along with DNA immunogens resulted in a significant enhancement of T-helper cell proliferative responses. In addition, coimmunization with pCICAM-1 (and more moderately with pCLFA-3) resulted in a dramatic enhancement of CD8-restricted cytotoxic T-lymphocyte responses. Although VCAM-1 and ICAM-1 are similar in size, VCAM-1 coimmunization did not have any measurable effect on cell-mediated responses. These results suggest that ICAM-1 and LFA-3 provide direct T-cell costimulation. These observations are further supported by the finding that coinjection with ICAM-1 dramatically enhanced the level of interferon-gamma (IFN-gamma) and beta-chemokines macrophage inflammatory protein-1alpha (MIP-1alpha), MIP-1beta, and regulated on activation normal T-cell expression and secreted (RANTES) produced by stimulated T cells. Through comparative studies, we observed that ICAM-1/LFA-1 T-cell costimulatory pathways are independent of CD86/CD28 pathways and that they may synergistically expand T-cell responses in vivo. (+info)
Crystal structure of the CD2-binding domain of CD58 (lymphocyte function-associated antigen 3) at 1.8-A resolution. (2/194)The binding of the cell surface molecule CD58 (formerly lymphocyte function-associated antigen 3) to its ligand, CD2, significantly increases the sensitivity of antigen recognition by T cells. This was the first heterophilic cell adhesion interaction to be discovered and is now an important paradigm for analyzing the structural basis of cell-cell recognition. The crystal structure of a CD2-binding chimeric form of CD58, solved to 1.8-A resolution, reveals that the ligand binding domain of CD58 has the expected Ig superfamily V-set topology and shares several of the hitherto unique structural features of CD2, consistent with previous speculation that the genes encoding these molecules arose via duplication of a common precursor. Nevertheless, evidence for considerable divergence of CD2 and CD58 is also implicit in the structures. Mutations that disrupt CD2 binding map to the highly acidic surface of the AGFCC'C" beta-sheet of CD58, which, unexpectedly, lacks marked shape complementarity to the equivalent, rather more basic CD58-binding face of human CD2. The specificity of the very weak interactions of proteins mediating cell-cell recognition may often derive largely from electrostatic complementarity, with shape matching at the protein-protein interface being less exact than for interactions that combine specificity with high affinity, such as those involving antibodies. (+info)
Functional glycan-free adhesion domain of human cell surface receptor CD58: design, production and NMR studies. (3/194)A general strategy is presented here for producing glycan-free forms of glycoproteins without loss of function by employing apolar-to-polar mutations of surface residues in functionally irrelevant epitopes. The success of this structure-based approach was demonstrated through the expression in Escherichia coli of a soluble 11 kDa adhesion domain extracted from the heavily glycosylated 55 kDa human CD58 ectodomain. The solution structure was subsequently determined and binding to its counter-receptor CD2 studied by NMR. This mutant adhesion domain is functional as determined by several experimental methods, and the size of its binding site has been probed by chemical shift perturbations in NMR titration experiments. The new structural information supports a 'hand-shake' model of CD2-CD58 interaction involving the GFCC'C" faces of both CD2 and CD58 adhesion domains. The region responsible for binding specificity is most likely localized on the C, C' and C" strands and the C-C' and C'-C" loops on CD58. (+info)
Structure of a heterophilic adhesion complex between the human CD2 and CD58 (LFA-3) counterreceptors. (4/194)Interaction between CD2 and its counterreceptor, CD58 (LFA-3), on opposing cells optimizes immune recognition, facilitating contacts between helper T lymphocytes and antigen-presenting cells as well as between cytolytic effectors and target cells. Here, we report the crystal structure of the heterophilic adhesion complex between the amino-terminal domains of human CD2 and CD58. A strikingly asymmetric, orthogonal, face-to-face interaction involving the major beta sheets of the respective immunoglobulin-like domains with poor shape complementarity is revealed. In the virtual absence of hydrophobic forces, interdigitating charged amino acid side chains form hydrogen bonds and salt links at the interface (approximately 1200 A2), imparting a high degree of specificity albeit with low affinity (K(D) of approximately microM). These features explain CD2-CD58 dynamic binding, offering insights into interactions of related immunoglobulin superfamily receptors. (+info)
Human endothelial cells augment early CD40 ligand expression in activated CD4+ T cells through LFA-3-mediated stabilization of mRNA. (5/194)Human endothelial cells (EC) augment CD40 ligand (CD40L) expression on PHA-activated CD4+ T cells at early times (e.g., 4-6 h). Fixed EC, devoid of mRNA, are comparable to living EC in their capacity to augment early CD40L expression on CD4+ T cells. Fixed EC increase T cell mRNA expression of both IL-2 and CD40L compared with PHA alone at 6 h. EC are unable to increase the rate of transcription of CD40L compared with PHA alone as measured with a promoter-reporter gene, although they do increase transcription of an IL-2 promoter-reporter gene. Fixed EC prolong the half-life of CD40L mRNA >2-fold. Inclusion of anti-human LFA-3 (CD58) mAb or pretreatment of EC with an LFA-3 antisense oligonucleotide blocks EC-induced increases in CD40L expression, whereas mAb to ICAM-1 or pretreatment with ICAM-1 antisense oligonucleotide does not. Moreover, mAb to LFA-3 reverses the capacity of EC to prolong the half-life of CD40L mRNA, whereas mAb to ICAM-1, even in combination with mAb to ICAM-2, does not. We conclude that EC use LFA-3 to increase early CD40L protein expression on newly activated CD4+ T cells by stabilizing CD40L mRNA. (+info)
A triad of costimulatory molecules synergize to amplify T-cell activation. (6/194)The activation of a T cell has been shown to require two signals via molecules present on professional antigen-presenting cells: signal 1, via a peptide/MHC complex; and signal 2, via a costimulatory molecule. Here, the role of three costimulatory molecules in the activation of T cells was examined. Poxvirus (vaccinia and avipox) vectors were used because of their ability to efficiently express multiple genes. Murine cells provided with signal 1 and infected with either recombinant vaccinia or avipox vectors containing a TRIad of COstimulatory Molecules (B7-1/ICAM-1/LFA-3, designated TRICOM) induced the activation of T cells to a far greater extent than cells infected with any one or two costimulatory molecules. Despite this T-cell "hyperstimulation" using TRICOM vectors, no evidence of apoptosis above that seen using the B7-1 vector was observed. Results using the TRICOM vectors were most dramatic under conditions of either low levels of first signal or low stimulator cell:T-cell ratios. Experiments using a four-gene construct also showed that TRICOM recombinants can enhance antigen-specific T-cell responses in vivo. These studies thus demonstrate for the first time the ability of vectors to introduce three costimulatory molecules into cells, thereby activating both CD4+ and CD8+ T-cell populations to levels greater than those achieved with the use of only one or two costimulatory molecules. This new threshold of T-cell activation has broad implications in vaccine design and development. (+info)
A space-time structure determination of human CD2 reveals the CD58-binding mode. (7/194)We describe a procedure for a space-time description of protein structures. The method is capable of determining populations of conformational substates, and amplitudes and directions of internal protein motions. This is achieved by fitting static and dynamic NMR data. The approach is based on the jumping-among-minima concept. First, a wide conformational space compatible with structural NMR data is sampled to find a large set of substates. Subsequently, intrasubstate motions are sampled by using molecular dynamics calculations with force field energy terms. Next, the populations of substates are fitted to NMR relaxation data. By diagonalizing a second moment matrix, directions and amplitudes of motions are identified. The method was applied to the adhesion domain of human CD2. We found that very few substates can account for most of the experimental data. Furthermore, only two types of collective motions have high amplitudes. They represent transitions between a concave (closed) and flat (open) binding face and resemble the change upon counter-receptor (CD58) binding. (+info)
Characterization of activated lymphocyte-tumor cell adhesion. (8/194)This study demonstrates the variable expression of ICAM-1 and leukocyte function antigen-3 (LFA-3) on four tumor cell lines (COLO526, K562, Daudi, and HT-29). In addition, phorbol ester (PMA) activation of lymphocytes modulated LFA-1 from a uniform to a clustered surface distribution; whereas after treatment with high levels of Mg2+ ions, the unique epitope for high-affinity LFA-1 was identified using clone Mab24. Using a flow cytometric adhesion assay it was demonstrated that PMA-activated lymphocytes formed conjugates with COLO526 and Daudi, and that these conjugates were inhibited by anti-CD2 with varying inhibition by LFA-1 clones MHM24 and 25.3.1. When lymphocytes were induced to express the high-affinity form of LFA-1, conjugates were identified with COLO526, K562, and Daudi and these conjugates were sensitive to the presence of both CD2 and LFA-1 antibodies. Further studies using confocal microscopy confirmed significant adhesion between peripheral blood lymphocytes pretreated with either PMA or high levels of Mg2+ and the adherent cell line COLO526. In conclusion, this unique study has demonstrated for the first time the important role of the active form of LFA-1 on the lymphocyte cell surface for conjugate formation with an ICAM-1-expressing tumor cell; also, two pathways of cell signaling were identified for conjugate formation to occur. (+info)
It interacts with other adhesion molecules, such as lymphocyte function-associated antigen-3 (LFA-3/CD58) in humans, or CD48 in ... CD2 Antigen at the US National Library of Medicine Medical Subject Headings (MeSH) Mouse CD Antigen Chart Human CD Antigen ... The great majority of T cell lymphomas and leukaemias also express CD2, making it possible to use the presence of the antigen ... Hahn WC, Menu E, Bothwell AL, Sims PJ, Bierer BE (1992). "Overlapping but nonidentical binding sites on CD2 for CD58 and a ...
... , or lymphocyte function-associated antigen 3 (LFA-3), is a cell adhesion molecule expressed on Antigen Presenting Cells ( ... CD58 Antigen at the US National Library of Medicine Medical Subject Headings (MeSH). ... Polymorphisms in the CD58 gene are associated with increased risk for multiple sclerosis. Barbosa JA, Mentzer SJ, Kamarck ME, ... This adhesion occurs as part of the transitory initial encounters between T cells and Antigen Presenting Cells before T cell ...
List of MeSH codes (D23)
... antigens, cd56 MeSH D23.050.301.264.035.157 --- antigens, cd57 MeSH D23.050.301.264.035.158 --- antigens, cd58 MeSH D23.050. ... antigens, cd56 MeSH D188.8.131.52.157 --- antigens, cd57 MeSH D184.108.40.206.158 --- antigens, cd58 MeSH D220.127.116.11.159 ... hla-a antigens MeSH D23.050.301.500.450.370.372 --- hla-a1 antigen MeSH D23.050.301.500.450.370.374 --- hla-a2 antigen MeSH ... hla-b antigens MeSH D23.050.301.500.450.380.383 --- hla-b7 antigen MeSH D23.050.301.500.450.380.385 --- hla-b8 antigen MeSH ...
Alan M. Krensky
Krensky AM, Robbins E, Springer TA, and Burakoff SJ: LFA-1, LFA-2, and LFA-3 antigens are involved in CTL-target conjugation. J ... CD58). Proc. Natl. Acad. Sci. USA 1982; 79: 7489-7493. "NIH Director Selects Dr. Alan M. Krensky as NIH Deputy Director for the ... Krensky AM, Weiss A, Crabtree G, Davis M, Parham P: Mechanisms of disease: T lymphocyte - antigen interactions in transplant ... Sanchez-Madrid F, Krensky AM, Ware CF, Robbins E, Strominger JL, Burakoff SJ, Springer TA: Three distinct antigens associated ...
Outline of immunology
Antigen Antigenicity Immunogen Superantigen Allergen Hapten Epitope Linear Conformational Mimotope Tumor antigen Antigen- ... CD2 family CD2 CD58 Signaling lymphocytic activation molecules (SLAMs) SLAMF1 (CD150) SLAMF2 (CD48) SLAMF3 (CD229, LY9) SLAMF4 ... T cells Antigen receptor - T cell receptor (TCR) Subunits - [email protected] / [email protected] / [email protected] / [email protected] Co-receptors CD8 (CD8α / CD8β) CD4 ... B cells Antigen receptor - B cell receptor (BCR) Subunits- Immunoglobulin heavy chain / Immunoglobulin light chain Co-receptors ...
1990). "The CD59 antigen is a structural homologue of murine Ly-6 antigens but lacks interferon inducibility". Eur. J. Immunol ... 1992). "Overlapping but nonidentical binding sites on CD2 for CD58 and a second ligand CD59". Science. 256 (5065): 1805-1807. ... CD59 Antigen at the US National Library of Medicine Medical Subject Headings (MeSH) Human CD59 genome location and CD59 gene ... 1990). "Isolation and expression of the full-length cDNA encoding CD59 antigen of human lymphocytes". DNA Cell Biol. 9 (3): 213 ...
"Replication of CD58 and CLEC16A as genome-wide significant risk genes for multiple sclerosis". Journal of Human Genetics. 54 ( ... Tissue Antigens. 73 (4): 326-9. doi:10.1111/j.1399-0039.2009.01216.x. PMID 19317741. Martínez A, Perdigones N, Cénit MC, Espino ...
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Crystal structure of the CD2-binding domain of CD58 (lymphocyte function-associated antigen 3) at 1.8-A resolution. - Oxford...
The binding of the cell surface molecule CD58 (formerly lymphocyte function-associated antigen 3) to its ligand, CD2, significantly increases the sensitivity of antigen recognition by T cells. This was the first heterophilic cell adhesion interaction to be discovered and is now an important paradigm for analyzing the structural basis of cell-cell recognition. The crystal structure of a CD2-binding chimeric form of CD58, solved to 1.8-A resolution, reveals that the ligand binding domain of CD58 has the expected Ig superfamily V-set topology and shares several of the hitherto unique structural features of CD2, consistent with previous speculation that the genes encoding these molecules arose via duplication of a common precursor. Nevertheless, evidence for considerable divergence of CD2 and CD58 is also implicit in the structures. Mutations that disrupt CD2 binding map to the highly acidic surface of the AGFCCC beta-sheet of CD58, which, unexpectedly, lacks marked shape complementarity to the equivalent
Studies on the role of lymphocyte function-associated antigen 1 (LFA-1) in T cell activation. - Semantic Scholar
The effect of polyclonal (anti alpha and beta chain) and monoclonal (anti alpha-chain) antibodies against lymphocyte function-associated antigen 1 (LFA-1) on T cell activation was studied. When added at the beginning of activation but not after 24 h or later the antibodies as well as the F(ab)2 or Fab fragments of polyclonal antibodies inhibited concanavalin A (Con A)-induced proliferation, interleukin 2 (IL-2) production, and the expression of receptors for IL-2 and transferrin. The inhibitory effect reached a maximum at the same time as optimal proliferation (72 h). Inhibition of proliferation lasted for 5 days or longer, although IL-2 production was only inhibited during the first 48 h of culture. Receptors for IL-2 and transferrin were re-expressed to the original level after 3 days of activation. Addition of external IL-2 at the beginning of the anti-LFA-1 containing culture prevented the inhibition of IL-2 receptor expression, while inhibition of transferrin receptor expression was unaffected,
transplant organs such as kidneys. They respond by recognising foreign antigens or peptides in the lymph nodes or sites of infection in the body. This leads to the proliferation of T cells and the expansion of reactive cells. The ability to respond depends on the movement of T-cells and length of time that T-cells spend attached to antigen presenting cells such as dendritic cells (DCs) that present the foreign antigen. Ligation of the antigen-receptor (also called the T-cell receptor or TCR) sends intracellular signals (termed the inside-out pathway) that slow T-cell motility allowing them to make stable contacts with DCs. Although essential for T cell function, the identity of the T cell receptor inside-out pathway for lymphocyte function-associated antigen 1 (LFA-1) adhesion has proved elusive.. As published this month in Immunity, the Rudd lab reports the identification of a novel inside-out pathway that is mediated by N-terminal SKAP1 (SKAP-55) domain binding to the C-terminal ...
Homo sapiens integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1; macrophage antigen 1 (mac-1) beta subunit) (ITGB2), mRNA. (H00003689-R02) - Products - Abnova
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The lymphocyte function-associated antigen 1 I domain is a transient binding module for intercellular adhesion molecule (ICAM)...
The I domain of lymphocyte function-associated antigen (LFA)-1 contains an intercellular adhesion molecule (ICAM)-1 and ICAM-3 binding site, but the relationship of this site to regulated adhesion is unknown. To study the adhesive properties of the LFA-1 I domain, we stably expressed a GPI-anchored form of this I domain (I-GPI) on the surface of baby hamster kidney cells. I-GPI cells bound soluble ICAM-1 (sICAM-1) with a low avidity and affinity. Flow cell experiments demonstrated a specific rolling interaction of I-GPI cells on bilayers containing purified full length ICAM-1 or ICAM-3. The LFA-1 activating antibody MEM-83, or its Fab fragment, decreased the rolling velocity of I-GPI cells on ICAM-1-containing membranes. In contrast, the interaction of I-GPI cells with ICAM-3 was blocked by MEM-83. Rolling of I-GPI cells was dependent on the presence of Mg2+. Mn2+ only partially substituted for Mg2+, giving rise to a small fraction of rolling cells and increased rolling velocity. This suggests that the
Lifitegrast is a non-steroidal, small molecule, integrin antagonist that inhibits lymphocyte function-associated antigen-1 (LFA-1; CD11a/CD18), which is being
Leukocyte function-associated antigen 1 (LFA-1) and CD44 are signalling molecules for cytoskeleton-dependent morphological...
Signaling through the leukocyte function-associated antigen 1 (LFA-1) molecule has previously been shown to induce homotypic aggregation in T cells and to induce cytoskeletal changes in T lymphoma cells. In this study we describe the induction of a d
Tricom Vaccine Delivered Safely With Smallpox, Avipox Vectors | Cancer Network | The Oncology Journal
MIAMI BEACH-Sequential Tri-com vaccines against cancers testing positive for carcinoembryonic antigen (CEA) have produced one clinical response and no toxicity among 23 patients in a phase I clinical trial. 1
The launch of an experimental fin-stabilized Japanese rocket with a shoebox-sized CubeSat was scrubbed Tuesday, pushing back a test flight to demonstrate how companies and institutions can inexpensively put small satellites in space. ...
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Purified lymphocyte function-associated antigen 3 binds to CD2 and mediates T lymphocyte adhesion. - Nuffield Department of...
CD2 is a T lymphocyte glycoprotein that functions in adhesion of T lymphocytes and also as a putative receptor for activation signals. Functional data suggest that LFA-3, a widely distributed cell surface glycoprotein, may be the biological ligand of CD2. We have purified LFA-3 from human erythrocytes and characterized the purified protein functionally. LFA-3 bound specifically to CD2+ cells, and this binding was inhibited by CD2 mAb. Conversely, purified LFA-3 inhibited binding of CD2 mAb to cells, and the concentration required for this effect suggests that LFA-3 half-saturated CD2 at 1-5 nM LFA-3. Purified LFA-3 inhibited rosetting of human and sheep erythrocytes with CD2+ T lymphoma cells and T lymphocytes, and mediated aggregation of a CD2+ T lymphoma cell line. Purified LFA-3 reconstituted into planar membranes mediated efficient CD2-dependent adhesion of T lymphoblasts. These data demonstrate that LFA-3 is a ligand for CD2 and that LFA-3 can mediate T lymphocyte adhesion.
Critical amino acids in the lymphocyte function-associated antigen-1 I domain mediate intercellular adhesion molecule 3 binding...
We have identified amino acid residues within the evolutionarily conserved I domain of the alpha-chain (CD11a) of the leukocyte integrin leukocyte function-associated antigen (LFA) 1 that are critical for intercellular adhesion molecule (ICAM) 3 (CD50) binding. ICAM-3, a ligand of LFA-1, is thought to mediate intercellular adhesion essential for the initiation of immune responses. Using a panel of human/murine I domain chimeras and point mutants, we observed that the Ile-Lys-Gly-Asn motif, located in the NH2-terminal part of the CD11a I domain, is required for ICAM-3 but not ICAM-1 binding. These findings demonstrate that the I domain of CD11a contains distinct functional subdomains for ligand specific binding. An aspartic acid located at position 137, which is essential to ICAM-1/LFA-1 interactions (Edwards, C.P., M. Champe, T. Gonzalez, M.E. Wessinger, S.A. Spencer, L.G. Presta, P.W. Berman, and S.C. Bodary. 1995. J. Biol. Chem. 270:12635-12640), was also critical for ICAM-3 binding, whereas ...
CD2 interacts with lymphocyte function-associated antigen (LFA-3) to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
This study aimed to determine the effects of dietary spermine supplementation on the inflammatory response and immune function of the thymus and spleen in piglets. Eighty suckling piglets were randomly assigned to receive adequate nutrients supplemented with spermine (0.4 mmol/kg body weight) or restricted nutrient intake supplemented with normal saline for 7 h or 3, 6 and 9 days in pairs. Regardless of treatment time, spermine supplementation decreased (p , 0.05, compared with the controls) the following: (1) tumour necrosis factor α (TNF-α), interleukin (IL)-1β, 2 and 6, and interferon (IFN)-γ levels in serum; (2) gene expression of cluster of differentiation 8 and integrin beta-2 in the thymus and spleen and the lymphocyte function-associated antigen 1 in the thymus; (3) mRNA levels of TNF-α, IL 1β, 2, 6, and 12, IFN-γ and inducible nitric oxide synthase in the thymus and spleen, as well as IL-8 in the spleen; and (4) eukaryotic IF4E-binding protein 1, Janus kinase 2, signal transducer ...
ICOS Corporation (a subsidiary of Eli Lilly) was developing small molecule antagonists of lymphocyte function-associated antigen-1 (LFA-1) as potential
CD2, also known as T-cell surface antigen CD2, is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. It interacts with other adhesion molecules, such as lymphocyte function-associated antigen-3 (LFA-3/CD58) in hum ...
CD2, also known as T-cell surface antigen CD2, is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. It interacts with other adhesion molecules, such as lymphocyte function-associated antigen-3 (LFA-3/CD58) in hum ...
En immunologie, une synapse immunologique (ou synapse immune) est linterface entre une cellule présentatrice dantigène ou cellule cible et un lymphocyte tel quun effecteur cellule T ou cellule Natural Killer. La synapse immunologique est aussi connue sous le nom de complexe supramoléculaire dadhésion ou SMAC (pour supramolecular adhesion complex en anglais). Cette structure est composée danneaux concentriques chacun contenant des groupes (clusters) séparés les uns les autres de protéines: c-SMAC (central-SMAC) composé de lisoforme θ isoform de la protéine kinase C, de CD2, CD4, CD8, CD28, Lck, et Fyn. p-SMAC (peripheral-SMAC) au sein duquel la protéine LFA-1 (Lymphocyte Function-associated Antigen-1) et la protéine du cytosquelette protéine taline sont agrégées. d-SMAC (distal-SMAC) enrichi en molécules CD43 et CD45,. Ce complexe dans son ensemble est supposé avoir plusieurs fonctions incluant entre autres (liste non exhaustive): Régulation de lactivation des ...
Integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide), also known as ITGAL, is a human gene which functions in the immune system. It is involved in cellular adhesion and costimulatory signaling. It is the target of the drug efalizumab.. ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling.. CD11a is one of the two components, along with CD18, which form lymphocyte function-associated antigen-1.. Efalizumab acts as an immunosuppressant by binding to ...
Discovery of novel p-arylthio cinnamides as antagonists of leukocyte function-associated antigen-1/intracellular adhesion...
Sigma-Aldrich offers abstracts and full-text articles by [G Liu, J T Link, Z Pei, E B Reilly, S Leitza, B Nguyen, K C Marsh, G F Okasinski, T W von Geldern, M Ormes, K Fowler, M Gallatin].
Lifitegrast is an integrin lymphocyte function-associated antigen-1 (LFA-1) antagonist; inhibits Jurkat T cell attachment to ICAM-1 with an IC50 of 2.98 nM. Buy Integrin inhibitor Lifitegrast from AbMole BioScience.
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Gentaur Molecular :Accu \ CD11a, LFA 1, alpha, Ly 15, Clone 121 7, Rat Mab anti Mouse, FITC \ ACL8916F-3
Gentaur molecular products has all kinds of products like :search , Accu \ CD11a, LFA_1, alpha, Ly 15, Clone 121_7, Rat Mab anti_Mouse, FITC \ ACL8916F-3 for more molecular products just contact us
Integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide), also known as ITGAL, is a human gene which functions in the immune system. It is involved in cellular adhesion and costimulatory signaling. It is the target of the drug efalizumab. ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. CD11a is one of the two components, along with CD18, which form lymphocyte function-associated antigen-1. Efalizumab acts as an immunosuppressant by binding to CD11a but was ...
Estradiol (E2) plays a key role in breast cancer progression. Most breast cancer recurrences express the estrogen receptor (ER), but nearly 50% of patients are resistant to anti-estrogen therapy. Novel therapeutic targets of ER positive breast cancers are needed. Protumoral neutrophils expressing the lymphocyte function-associated antigen 1 (LFA-1) integrin may mediate cancer metastasis and transforming growth factor β1 (TGFβ1) is the major chemoattractant for neutrophils. The role of E2 in neutrophil-ER+ breast cancer cell interactions is unknown. We studied this in vivo using murine breast cancers in immunocompetent mice and human breast cancers in nude mice. Cell dissemination was evaluated in a zebrafish model, and microdialysis of breast cancer patients was performed. In vitro studies were done with mammosphere cultures of breast cancer cells and human neutrophils. We found that E2 increased the number of LFA-1+ neutrophils recruited to the invasive edge of mouse tumors, increased TGFβ1 ...
In recent years, small interference RNAs (siRNAs) have greatly enhanced our understanding of protein functions by allowing knockdown of targeted proteins at the mRNA level
Differential effects of polyphenols and alcohol of red wine on the expression of adhesion molecules and inflammatory cytokines...
Background: Few clinical studies have focused on the alcoholindependent cardiovascular effects of the phenolic compounds of red wine (RW). Objective: We aimed to evaluate the effects of ethanol and phenolic compounds of RW on the expression of inflammatory biomarkers related to atherosclerosis in subjects at high risk of cardiovascular disease. Design: Sixty-seven high-risk, male volunteers were included in a randomized, crossover consumption trial. After a washout period, all subjects received RW (30 g alcohol/d), the equivalent amount of dealcoholized red wine (DRW), or gin (30 g alcohol/d) for 4 wk. Before and after each intervention period, 7 cellular and 18 serum inflammatory biomarkers were evaluated. Results: Alcohol increased IL-10 and decreased macrophage-derived chemokine concentrations, whereas the phenolic compounds of RW decreased serum concentrations of intercellular adhesion molecule- 1, E-selectin, and IL-6 and inhibited the expression of lymphocyte function-associated antigen 1 ...
Force-induced formation and propagation of adhesion nanodomains in living fungal cells. | DIAL.pr - BOREAL
Understanding how cell adhesion proteins form adhesion domains is a key challenge in cell biology. Here, we use single-molecule atomic force microscopy (AFM) to demonstrate the force-induced formation and propagation of adhesion nanodomains in living fungal cells, focusing on the covalently anchored cell-wall protein Als5p from Candida albicans. We show that pulling on single adhesins with AFM tips terminated with specific antibodies triggers the formation of adhesion domains of 100-500 nm and that the force-induced nanodomains propagate over the entire cell surface. Control experiments (with cells lacking Als5p, single-site mutation in the protein, bare tips, and tips modified with irrelevant antibodies) demonstrate that Als5p nanodomains result from protein redistribution triggered by force-induced conformational changes in the initially probed proteins, rather than from nonspecific cell-wall perturbations. Als5p remodeling is independent of cellular metabolic activity because heat-killed ...
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International Journal of Cancer Immunology & Immunotherapy, An Open Access Journal | Graphy Publications
International Journal of Cancer Immunology & Immunotherapy (IJCII) is an international peer reviewed journal devoted towards cancer immuology & immunotherapy. All the articles published under IJCII, include high quality papers, which cover all the major field autoimmune disorders & therapy. IJCII keeps updating day-to-day research & development to the scientific niche around the world. The journal publishes original articles, commentary, editorials, letters to the editor, review articles and case report describing original research in the fields of cancer immunology.
Lifitegrast (trade name Xiidra) is an FDA approved drug indicated for the treatment of signs and symptoms of dry eye, a syndrome called keratoconjunctivitis sicca. Lifitegrast reduces inflammation by inhibiting inflammatory cell binding. Common side effects in clinical trials were eye irritation, discomfort, blurred vision, and dysgeusia (a distortion of the sense of taste). Lifitegrast is supplied as an eye drop that can be applied two times a day. Lifitegrast inhibits an integrin, lymphocyte function-associated antigen 1 (LFA-1), from binding to intercellular adhesion molecule 1 (ICAM-1). This mechanism down-regulates inflammation mediated by T lymphocytes. Lifitegrast was initially designed and developed by SARcode Bioscience which was acquired by Shire in 2013, who submitted a new drug application to the US Food and Drug Administration (FDA) in March 2015. The FDA granted Shire a priority review a month later, and requested additional clinical data, which were supplied in January 2016. The ...
#promius pharma #Promius Pharma, LLC Latest Drug Information Updates Troxyca ER Troxyca ER (oxycodone hydrochloride and naltrexone hydrochloride) is an extended-release, abuse-deterrent. Adlyxin Adlyxin (lixisenatide) is a once-daily prandial glucagon-like peptide-1 (GLP-1) receptor agonist indicated. Xiidra Xiidra (lifitegrast) is a lymphocyte function-associated antigen-1 (LFA-1) antagonist indicated for the. Epclusa Epclusa (sofosbuvir and velpatasvir) is a…
#tris pharma #Tris Pharma, Inc. Latest Drug Information Updates Troxyca ER Troxyca ER (oxycodone hydrochloride and naltrexone hydrochloride) is an extended-release, abuse-deterrent. Adlyxin Adlyxin (lixisenatide) is a once-daily prandial glucagon-like peptide-1 (GLP-1) receptor agonist indicated. Xiidra Xiidra (lifitegrast) is a lymphocyte function-associated antigen-1 (LFA-1) antagonist indicated for the. Epclusa Epclusa (sofosbuvir and velpatasvir) is a…
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DISCONTINUED: LFA 447 NanoFlash® - Xenon Flash Apparatus from NETZSCH-Gerätebau GmbH | SelectScience
Read independent reviews on DISCONTINUED: LFA 447 NanoFlash® - Xenon Flash Apparatus from NETZSCH-Gerätebau GmbH on SelectScience
KEGG BRITE: CD Molecules - Homo sapiens (human)
... neural cell adhesion molecule K06492 CD58; CD58 antigen K04008 CD59; CD59 antigen K06493 ITGB3; integrin beta 3 K06494 SELE; ... CD79A antigen K06507 CD79B; CD79B antigen K05412 CD80; CD80 antigen K06508 CD81; CD81 antigen K06509 KAI1; CD82 antigen K06510 ... CD300 antigen K06719 CD300; CD300 antigen K06719 CD300; CD300 antigen K06719 CD300; CD300 antigen K06721 CLEC10A; C-type lectin ... CD96 antigen K08446 ADGRE5; CD97 antigen K06519 SLC3A2; solute carrier family 3, member 2 K06520 CD99; CD99 antigen K06521 ...https://www.genome.jp/kegg-bin/get_htext?hsa04090+4486
Code System Concept
Cluster of differentiation antigen 58 Current Synonym true false 1222120012 CD58 - Cluster of differentiation antigen 58 ... LFA-3 - Lymphocyte function-associated antigen-3 Current Synonym true false 31103010 Lymphocyte antigen CD58 Current Synonym ... Lymphocyte function-associated antigen-3 Current Synonym true false 1222122016 ... Lymphocyte antigen CD58 (substance). Code System Preferred Concept Name. Lymphocyte antigen CD58 (substance). ...https://phinvads.cdc.gov/vads/ViewCodeSystemConcept.action?oid=2.16.840.1.113883.6.96&code=18436004
CD58 - Beckman Coulter
CD58 was first described as the lymphocyte-function associated antigen 3 (LFA-3). ... CD58 is a glycoprotein of 65-70 kDa, either transmembrane or glycosylphosphatidylinositol (GPI)-anchored. ... CD58 Antigen. CD58 is a glycoprotein of 65-70 kDa, either transmembrane or glycosylphosphatidylinositol (GPI)-anchored. CD58 ... CD58 activate the costimulation pathways of T lymphocytes and natural killer (NK) cells, maximizing the cytolysis of target ...https://www.beckman.com.au/reagents/coulter-flow-cytometry/antibodies-and-kits/single-color-antibodies/cd58
US20030003098A1 - Inhibiting rejection of a graft - Google Patents
... chimeras having enzymatically inactive polypeptides bonded to polypeptides which bind to co-stimulatory proteins of antigen- ... The methods involve treating the graft with a molecule which binds to a co-stimulatory protein of antigen-presenting cells. ... 108010084313 CD58 Antigens Proteins 0 Claims Description 7 * 108010087819 Fc Receptors Proteins 0 Claims Description 15 ... 102000016266 T-Cell Antigen Receptors Human genes 0 Description 5 * 108010092262 T-Cell Antigen Receptors Proteins 0 ...https://patents.google.com/patent/US20030003098A1/en
SangKon Oh, PhD - Research Output - Mayo Clinic
Concomitant activation and antigen uptake via human Dectin-1 results in potent antigen-specific CD8+ T cell responses. Ni, L., ... Targeting concatenated HIV antigens to human CD40 expands a broad repertoire of multifunctional CD4+ and CD8+ T cells. Flamar, ... Induction and activation of human Th17 by targeting antigens to dendritic cells via Dectin-1. Duluc, D., Joo, H., Ni, L., Yin, ... Functional Specialty of CD40 and Dendritic Cell Surface Lectins for Exogenous Antigen Presentation to CD8+ and CD4+ T Cells. ...https://mayoclinic.pure.elsevier.com/en/persons/sangkon-oh/publications/?type=%2Fdk%2Fatira%2Fpure%2Fresearchoutput%2Fresearchoutputtypes%2Fcontributiontojournal%2Farticle
Louis Picker - Publications - Oregon Health & Science University
Discordant expression of CD3 and T-cell receptor beta-chain antigens in T-lineage lymphomas. Picker, L., Brenner, M. B., Weiss ... Antigen-presenting capabilities of human monocytes correlates with their expression of HLA-DS, an Ia determinant distinct from ... Co-expression of L60 (Leu-22) and L26 antigens correlates with malignant histologic findings. Ngan, B. Y., Picker, L., Medeiros ... Expression of lymphocyte homing receptor antigen in non-Hodgkins lymphoma. Picker, L., Medeiros, L. J., Weiss, L. M., Warnke, ...https://ohsu.pure.elsevier.com/en/persons/louis-picker/publications/?type=%2Fdk%2Fatira%2Fpure%2Fresearchoutput%2Fresearchoutputtypes%2Fcontributiontojournal%2Farticle&page=3
CD58 - Wikipedia
CD58, or lymphocyte function-associated antigen 3 (LFA-3), is a cell adhesion molecule expressed on Antigen Presenting Cells ( ... CD58 Antigen at the US National Library of Medicine Medical Subject Headings (MeSH). ... Polymorphisms in the CD58 gene are associated with increased risk for multiple sclerosis. Barbosa JA, Mentzer SJ, Kamarck ME, ... This adhesion occurs as part of the transitory initial encounters between T cells and Antigen Presenting Cells before T cell ...https://en.wikipedia.org/wiki/CD58
CD2 - T-cell surface antigen CD2 precursor - Homo sapiens (Human) - CD2 gene & protein
CD2 interacts with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and ... CD2 interacts with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and ... T-cell surface antigen CD2Add BLAST. 327. Amino acid modifications. Feature key. Position(s). DescriptionActions. Graphical ... "Molecular cloning of the CD2 antigen, the T-cell erythrocyte receptor, by a rapid immunoselection procedure.". Seed B., Aruffo ...https://www.uniprot.org/uniprot/P06729
Association of CD58 gene polymorphisms with NMO spectrum disorders in a Han Chinese population. - PubMed - NCBI
Association of CD58 gene polymorphisms with NMO spectrum disorders in a Han Chinese population.. Liu J1, Shi Z1, Lian Z1, Chen ... CD58 Antigens/genetics*. *Female. *Genetic Association Studies/methods*. *Genetic Variation/genetics. *Humans ... This study aimed to perform a comprehensive assessment of the association between CD58 polymorphisms and the risk of ... In conclusion, this study identified a new NMOSD susceptibility variant, rs56302466, and suggested that CD58 polymorphisms are ...https://phgkb.cdc.gov/PHGKB/phgHome.action?action=forward&dbsource=huge&id=151145
Gendoo - Relevant features
Antigens, CD58 CD58抗原 Cadherins カドヘリン Adaptor Protein Complex alpha Subunits アダプター蛋白質複合体アルファサブユニット ... Receptors, Antigen, T-Cell, alpha-beta アルファ-ベータT細胞抗原http://gendoo.dbcls.jp/cgi-bin/gendoo.cgi?geneid=30011&taxonomy=human
Code System Concept
Lymphocyte positive for CD58 antigen (cell). Code System Preferred Concept Name. Lymphocyte positive for CD58 antigen (cell). ...https://phinvads.cdc.gov/vads/ViewCodeSystemConcept.action?oid=2.16.840.1.113883.6.96&code=117391008
Statins and Musculoskeletal Conditions, Arthropathies, and Injuries - American College of Cardiology
Antigens, CD58, Tendon Injuries, Myalgia ...http://www.acc.org/latest-in-cardiology/journal-scans/2013/06/03/16/34/statins-and-musculoskeletal-conditions-arthropathies-and-injuries
The lymphocyte associated antigen 3 (LFA-3, CD58) has been found in soluble form (sCD58) in human sera.  ... The complement inhibitor CD59 and the lymphocyte function-associated antigen-3 (LFA-3, CD58) genes possess functional binding ... The presence of LFA-3 on dendritic cells is consistent with the specialised function of these cells in presenting antigen to ... LFA-3 (CD58) mediates T-lymphocyte adhesion in chronic inflammatory infiltrates.  ...http://endonet.bioinf.med.uni-goettingen.de/hormone/ENH00668
Stem Cell | Recombinant Protein | cDNA
FGF4 FOSL1 GATA2 GBP2 GLI2 NHLH2 CD34 DLX6 IFIT2 IGSF6 IHH ICOS FGF3 IFI30 IFI35 IFIT2 KRT15 LILRB4 MYCL1 FCER2 FCGR2B CD58 HLA ... Lymphocyte function-associated antigen 3 (Ag3) (Surface glycoprotein LFA-3) (CD antigen CD58). ... 1. Viral Antigen ,,, 2. Bacterial Antigen ,,, 3. Kinase ,,,. 4. Bacterial Toxin ,,, 5. Parasite Antigen ,,,. 6. Cancer-related ... CAMPATH-1 antigen (Cambridge pathology 1 antigen) (Epididymal secretory protein E5) (CDw52) (CD antigen CD52). ...http://bioclone.us/stem-cell-cDNA-recombinant-protein-1.html
CD58 polymorphisms associated with the risk of neuromyelitis optica in a Korean population. - PubMed - NCBI
Schematic physical map, haplotypes and LD status of CD58 polymorphisms. (A) Polymorphisms identified in CD58. Coding exons are ... The analysis results showed that 6 variations (rs2300747, rs1335532, rs12044852, rs1016140, CD58_ht1, and CD58_ht3) showed ... CD58 polymorphisms associated with the risk of neuromyelitis optica in a Korean population.. Kim JY, Bae JS, Kim HJ, Shin HD1. ... CD58 Antigens. LinkOut - more resources. Full Text Sources. *BioMed Central. *Europe PubMed Central ...https://phgkb.cdc.gov/PHGKB/phgHome.action?action=forward&dbsource=huge&id=93268
LFA3抗体[MEM-63] (FITC)| Abcam中国
CD58 antigen (lymphocyte function associated antigen 3)1 antibody. *CD58 antigen antibody ... This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and - ... dependent interactions of T-lymphocytes with target cells and antigen-presenting cells and the T-lymphocyte rosetting with ...http://www.abcam.cn/lfa3-antibody-mem-63-fitc-ab26006.html
The coreceptor CD2 uses plasma membrane microdomains to transduce signals in T cells.
Antigen-Presenting Cells * Antigens, CD * Antigens, CD2 * Antigens, CD58 * CD2 Antigens * CD58 Antigens ... The interaction between a T cell and an antigen-presenting cell (APC) can trigger a signaling response that leads to T cell ... We show that the binding of CD58 to CD2, in the absence of TCR activation, also induces signaling through the actin-dependent ... In this study, we investigated another ligand-receptor interaction (CD58-CD2) that facilities T cell activation using a model ...http://vivo.mblwhoilibrary.org/display/publication29648
Alefacept in Patients With Severe Scalp Alopecia Areata - Full Text View - ClinicalTrials.gov
... lymphocyte function-associated antigen-3 CD58). Induction of a molecular signaling cascade with resultant T-cell activation and ... Activation of T-cells is initiated by interaction of the T-cell receptor with the antigen/major histocompatibility complex on ... Co-stimulatory interactions occur secondarily, including binding of the T-cell CD2 receptor to the antigen-presenting cell ... The cellular infiltrate primarily consists of activated T-lymphocytes and antigen-presenting Langerhans cells. T-lymphocytes ...https://clinicaltrials.gov/show/NCT00167102
WikiGenes - CD2 - CD2 molecule
Amino acid residues required for binding of lymphocyte function-associated antigen 3 (CD58) to its counter-receptor CD2. Osborn ... We now report that CD2 binds to a cell-surface antigen known as lymphocyte function-associated antigen-3 (LFA-3) with high ... Two antigen-independent adhesion pathways used by human cytotoxic T-cell clones. Shaw, S., Luce, G.E., Quinones, R., Gress, R.E ... Interaction between human CD2 and CD58 involves the major beta sheet surface of each of their respective adhesion domains. ...https://www.wikigenes.org/e/gene/e/914.html
CD2 Antibody (BH1) [DyLight 650] (NBP2-34561C): Novus Biologicals
CD2/CD58 binding can enhance antigen-specific T cell activation. CD2 is a transmembrane glycoprotein that is expressed on ... CD58 is a heavily glycosylated protein with a broad tissue distribution in hematopoietic and other cells, including endothelium ... CD2 interacts through its amino-terminal domain with the extracellular domain of CD58 (also designated CD2 ligand) to mediate ... Interaction between CD2 and its counter receptor LFA3 (CD58) on opposing cells optimizes immune system recognition, thereby ...https://www.novusbio.com/products/cd2-antibody-bh1_nbp2-34561c
Analysis of two-dimensional dissociation constant of laterally mobile cell adhesion molecules. - The Kennedy Institute of...
Both CD2 and CD58 are laterally mobile in their respective membranes. Adhesion is indicated by accumulation of CD2 and CD58 in ... We derive a new binding equation, B/F=[(Ntxf)/(KdxScell)]-[(Bxp)/Kd], where B and F are bound and free CD58 density in the ... We use this analysis to determine that the 2D Kd for CD2-CD58 is 5.4-7.6 molecules/microm2. 2D Kd analysis provides a general ... to study the interaction of CD2-expressing T cells with glass-supported planar bilayers containing fluorescently labeled CD58, ...https://www.kennedy.ox.ac.uk/publications/482603
P2X Receptors | Inhibition of DNA Glycosylases via Small Molecules
CD2 antigen also functions as the receptor for the CD58 antigen(LFA-3). progression1. However some tumours contain a relatively ... Normal B lymphocytes, monocytes or granulocytes do not express surface CD2 antigen, neither do common ALL cells. CD2 antigen ... Keywords: Biopsy Prostate-specific antigen Prostatitis Launch Prostate-specific antigen (PSA) may be the most commonly utilized ... Normal B lymphocytes, monocytes or granulocytes do not express surface CD2 antigen, Mouse monoclonal to CD2.This recognizes a ...http://cancer-colorectal.com/category/p2x-receptors/
CD2 Monoclonal Mouse Antibody (LFA2/600) | Biotium
CD2/CD58 binding can enhance antigen-specific T cell activation. CD2 is a transmembrane glycoprotein that is expressed on ... CD58 is a heavily glycosylated protein with a broad tissue distribution in hematopoietic and other cells, including endothelium ... CD2 interacts through its amino-terminal domain with the extracellular domain of CD58 (also designated CD2 ligand) to mediate ... Interaction between CD2 and its counter receptor LFA3 (CD58) on opposing cells optimizes immune system recognition, thereby ...https://biotium.com/product/monoclonal-anti-cd2-lfa2600/
Mouse Monoclonal anti-CD2 (HuLy-m1) | Biotium
CD2/CD58 binding can enhance antigen-specific T cell activation. CD2 is a transmembrane glycoprotein that is expressed on ... Activation of human T lymphocytes via the CD2 antigen results in tyrosine phosphorylation of T cell antigen receptor zeta- ... T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5; T11 ... CD58 is a heavily glycosylated protein with a broad tissue ... CD2 interacts through its amino-terminal domain with the extracellular domain of CD58 (also designated CD2 ligand) to mediate ...https://biotium.com/product/monoclonal-anti-cd2-huly-m1/
Albert Edge | Harvard Catalyst Profiles | Harvard Catalyst
... identification of a porcine antigen homologous to CD58. Transplantation. 2004 Apr 27; 77(8):1288-94. PMID: 15114100. ... antagonistic antibody AL-57 that preferentially binds the high-affinity form of lymphocyte function-associated antigen-1. J ...https://connects.catalyst.harvard.edu/profiles/display/Person/60563
Association between CD58 polymorphismsLymphocyte-associated antigenReceptorsLymphocytesMediates T-lymphocyte adhesionPolymorphismsLigandAdhesionCD48LFA3Dendritic CellsProteinCounter receptorGlycoproteinMembranesAntibodyInteractionMultiple sclerosisTumorWidely expressedSusceptibilityHumanModelActivationContact areaExpressionStudyAnalysis
- This study aimed to perform a comprehensive assessment of the association between CD58 polymorphisms and the risk of neuromyelitis optica spectrum disorders (NMOSD) in a Han Chinese population. (cdc.gov)
- Logistic regression analysis was conducted to find a possible association between CD58 polymorphisms and NMO. (cdc.gov)
- CD58 activate the costimulation pathways of T lymphocytes and natural killer (NK) cells, maximizing the cytolysis of target cells by cytotoxic T lymphocytes (CTL) and NK cells. (beckman.com.au)
- This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen-presenting cells and the T-lymphocyte rosetting with erythrocytes. (abcam.cn)
- The cellular infiltrate primarily consists of activated T-lymphocytes and antigen-presenting Langerhans cells. (clinicaltrials.gov)
- Interaction between CD2 and its counter receptor LFA3 (CD58) on opposing cells optimizes immune system recognition, thereby facilitating communication between helper T lymphocytes and antigen-presenting cells, as well as between cytolytic effectors and target cells. (novusbio.com)
- Polymorphisms in the CD58 gene are associated with increased risk for multiple sclerosis. (wikipedia.org)
- Association of CD58 gene polymorphisms with NMO spectrum disorders in a Han Chinese population. (cdc.gov)
- In conclusion, this study identified a new NMOSD susceptibility variant, rs56302466, and suggested that CD58 polymorphisms are associated with the risk of NMOSD in Han Chinese. (cdc.gov)
- CD58 polymorphisms associated with the risk of neuromyelitis optica in a Korean population. (cdc.gov)
- Thus, this study aimed to investigate the association of CD58 polymorphisms with the risk of NMO in a Korean population. (cdc.gov)
- Polymorphisms identified in CD58 . (cdc.gov)
- In this study, we investigated another ligand-receptor interaction (CD58-CD2) that facilities T cell activation using a model system consisting of Jurkat T cells interacting with a planar lipid bilayer that mimics an APC. (mblwhoilibrary.org)
- Co-stimulatory interactions occur secondarily, including binding of the T-cell CD2 receptor to the antigen-presenting cell ligand LFA-3 (lymphocyte function-associated antigen-3 CD58). (clinicaltrials.gov)
- CD2 interacts through its amino-terminal domain with the extracellular domain of CD58 (also designated CD2 ligand) to mediate cell adhesion. (novusbio.com)
- In solution, the interaction of human CD2 with its ligand CD58 is of low affinity (500 mM-1) and the interaction of rat CD2 with its ligand CD48 is of still lower affinity (40 mM-1). (ox.ac.uk)
- CD58, or lymphocyte function-associated antigen 3 (LFA-3), is a cell adhesion molecule expressed on Antigen Presenting Cells (APC), particularly macrophages. (wikipedia.org)
- It binds to CD2 (LFA-2) on T cells and is important in strengthening the adhesion between the T cells and Professional Antigen Presenting Cells. (wikipedia.org)
- CD2 interacts with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. (uniprot.org)
- A model for CD2/CD58-mediated adhesion strengthening. (ox.ac.uk)
- Yet how low affinity adhesion molecules such as CD2 and CD58 can produce stable cell adhesion is still not completely understood. (ox.ac.uk)
- In this phase I study, we administered one or two cycles of four triweekly s.c./intradermal injections of ex vivo generated dendritic cells modified with a recombinant fowlpox vector encoding carcinoembryonic antigen (CEA) and a triad of costimulatory molecules [rF-CEA(6D)-TRICOM]. (aacrjournals.org)
- Because of the crucial role of dendritic cells in adaptive immunity and their potent activity in animal tumor models, numerous pilot studies have evaluated immunotherapy with dendritic cells loaded with antigen in the form of peptide, protein, DNA, mRNA, tumor lysates, tumor fusions, and viral vectors ( 1 ). (aacrjournals.org)
- To overcome these limitations, we have been studying strategies for genetic modification of dendritic cells with viral vectors encoding full length tumor antigens and costimulatory molecules. (aacrjournals.org)
- The complement inhibitor CD59 and the lymphocyte function-associated antigen-3 (LFA-3, CD58) genes possess functional binding sites for the p53 tumor suppressor protein. (uni-goettingen.de)
- Binding of the immunoglobulin portion of the fusion protein to the FCy receptor on antigen-presenting cells potentiates apoptosis of CD-2 T-cells to thereby reduce the population of activated T-cells. (clinicaltrials.gov)
- CD58 is a heavily glycosylated protein with a broad tissue distribution in hematopoietic and other cells, including endothelium. (novusbio.com)
- The interaction between a T cell and an antigen-presenting cell (APC) can trigger a signaling response that leads to T cell activation. (mblwhoilibrary.org)
- Activation of T-cells is initiated by interaction of the T-cell receptor with the antigen/major histocompatibility complex on the antigen-presenting cells. (clinicaltrials.gov)
- It prevents co-stimulatory signals between antigen presenting cells and memory T cells by competitive inhibition of CD2 in T cells, induces selective apoptosis of CD4+ and CD8+ memory effector T cells by interaction between the Fc portion of IgG1 and the FcyIII in NK cells, and possibly direct ligation of CD2 molecules on T cells that subsequently result in the alteration in T cell agonist signaling. (clinicaltrials.gov)
- To determine the safety and immunologic and clinical efficacy of a dendritic cell vaccine modified to hyperexpress costimulatory molecules and tumor antigen. (aacrjournals.org)
- Among the many tumor antigens described, the model antigen, carcinoembryonic antigen (CEA), is of particular interest because it is widely expressed in gastrointestinal, lung, breast, and other malignancies. (aacrjournals.org)
- Both have been engineered to express tumor antigen, such as CEA, and have been tested in murine ( 10 ) and human ( 11 , 12 ) studies. (aacrjournals.org)
- We show that the binding of CD58 to CD2, in the absence of TCR activation, also induces signaling through the actin-dependent coalescence of signaling molecules (including TCR-zeta chain, Lck, and LAT) into microdomains. (mblwhoilibrary.org)
- CD2/CD58 binding can enhance antigen-specific T cell activation. (novusbio.com)
- A common goal of cancer vaccines in development is the activation of high levels of antigen-specific T cells. (aacrjournals.org)
- The analysis results showed that 6 variations (rs2300747, rs1335532, rs12044852, rs1016140, CD58_ht1, and CD58_ht3) showed significant associations (P = 0.002 ~ 0.008, P(corr) = 0.01 ~ 0.04). (cdc.gov)
- We use this analysis to determine that the 2D Kd for CD2-CD58 is 5.4-7.6 molecules/microm2. (ox.ac.uk)