Substances that are recognized by the immune system and induce an immune reaction.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
Differentiation antigens found on thymocytes and on cytotoxic and suppressor T-lymphocytes. CD8 antigens are members of the immunoglobulin supergene family and are associative recognition elements in MHC (Major Histocompatibility Complex) Class I-restricted interactions.
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
Substances elaborated by bacteria that have antigenic activity.
A bifunctional enzyme that catalyzes the synthesis and HYDROLYSIS of CYCLIC ADP-RIBOSE (cADPR) from NAD+ to ADP-RIBOSE. It is a cell surface molecule which is predominantly expressed on LYMPHOID CELLS and MYELOID CELLS.
Glycoproteins found on immature hematopoietic cells and endothelial cells. They are the only molecules to date whose expression within the blood system is restricted to a small number of progenitor cells in the bone marrow.
Differentiation antigens expressed on B-lymphocytes and B-cell precursors. They are involved in regulation of B-cell proliferation.
A member of the tumor necrosis factor receptor superfamily with specificity for CD40 LIGAND. It is found on mature B-LYMPHOCYTES and some EPITHELIAL CELLS, lymphoid DENDRITIC CELLS. Evidence suggests that CD40-dependent activation of B-cells is important for generation of memory B-cells within the germinal centers. Mutations of the gene for CD40 antigen result in HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 3. Signaling of the receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
A membrane glycoprotein and differentiation antigen expressed on the surface of T-cells that binds to CD40 ANTIGENS on B-LYMPHOCYTES and induces their proliferation. Mutation of the gene for CD40 ligand is a cause of HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 1.
Unglycosylated phosphoproteins expressed only on B-cells. They are regulators of transmembrane Ca2+ conductance and thought to play a role in B-cell activation and proliferation.
Substances elaborated by viruses that have antigenic activity.
Costimulatory T-LYMPHOCYTE receptors that have specificity for CD80 ANTIGEN and CD86 ANTIGEN. Activation of this receptor results in increased T-cell proliferation, cytokine production and promotion of T-cell survival.
Acidic sulfated integral membrane glycoproteins expressed in several alternatively spliced and variable glycosylated forms on a wide variety of cell types including mature T-cells, B-cells, medullary thymocytes, granulocytes, macrophages, erythrocytes, and fibroblasts. CD44 antigens are the principle cell surface receptors for hyaluronate and this interaction mediates binding of lymphocytes to high endothelial venules. (From Abbas et al., Cellular and Molecular Immunology, 2d ed, p156)
Differentiation antigens expressed on pluripotential hematopoietic cells, most human thymocytes, and a major subset of peripheral blood T-lymphocytes. They have been implicated in integrin-mediated cellular adhesion and as signalling receptors on T-cells.
Glycolipid-anchored membrane glycoproteins expressed on cells of the myelomonocyte lineage including monocytes, macrophages, and some granulocytes. They function as receptors for the complex of lipopolysaccharide (LPS) and LPS-binding protein.
Glycoprotein members of the immunoglobulin superfamily which participate in T-cell adhesion and activation. They are expressed on most peripheral T-lymphocytes, natural killer cells, and thymocytes, and function as co-receptors or accessory molecules in the T-cell receptor complex.
Ratio of T-LYMPHOCYTES that express the CD4 ANTIGEN to those that express the CD8 ANTIGEN. This value is commonly assessed in the diagnosis and staging of diseases affecting the IMMUNE SYSTEM including HIV INFECTIONS.
Glycoproteins expressed on all mature T-cells, thymocytes, and a subset of mature B-cells. Antibodies specific for CD5 can enhance T-cell receptor-mediated T-cell activation. The B-cell-specific molecule CD72 is a natural ligand for CD5. (From Abbas et al., Cellular and Molecular Immunology, 2d ed, p156)
Antigens expressed primarily on the membranes of living cells during sequential stages of maturation and differentiation. As immunologic markers they have high organ and tissue specificity and are useful as probes in studies of normal cell development as well as neoplastic transformation.
A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.
Glycoproteins expressed on cortical thymocytes and on some dendritic cells and B-cells. Their structure is similar to that of MHC Class I and their function has been postulated as similar also. CD1 antigens are highly specific markers for human LANGERHANS CELLS.
Antibodies produced by a single clone of cells.
The 140 kDa isoform of NCAM (neural cell adhesion molecule) containing a transmembrane domain and short cytoplasmic tail. It is expressed by all lymphocytes mediating non-MHC restricted cytotoxicity and is present on some neural tissues and tumors.
Antigens expressed on the cell membrane of T-lymphocytes during differentiation, activation, and normal and neoplastic transformation. Their phenotypic characterization is important in differential diagnosis and studies of thymic ontogeny and T-cell function.
A membrane-bound or cytosolic enzyme that catalyzes the synthesis of CYCLIC ADP-RIBOSE (cADPR) from nicotinamide adenine dinucleotide (NAD). This enzyme generally catalyzes the hydrolysis of cADPR to ADP-RIBOSE, as well, and sometimes the synthesis of cyclic ADP-ribose 2' phosphate (2'-P-cADPR) from NADP.
Surface antigens expressed on myeloid cells of the granulocyte-monocyte-histiocyte series during differentiation. Analysis of their reactivity in normal and malignant myelomonocytic cells is useful in identifying and classifying human leukemias and lymphomas.
A costimulatory ligand expressed by ANTIGEN-PRESENTING CELLS that binds to CTLA-4 ANTIGEN with high specificity and to CD28 ANTIGEN with low specificity. The interaction of CD80 with CD28 ANTIGEN provides a costimulatory signal to T-LYMPHOCYTES, while its interaction with CTLA-4 ANTIGEN may play a role in inducing PERIPHERAL TOLERANCE.
Tetraspanin proteins found at high levels in cells of the lymphoid-myeloid lineage. CD53 antigens may be involved regulating the differentiation of T-LYMPHOCYTES and the activation of B-LYMPHOCYTES.
A cell adhesion protein that was originally identified as a heat stable antigen in mice. It is involved in METASTASIS and is highly expressed in many NEOPLASMS.
Zinc-binding metalloproteases that are members of the type II integral membrane metalloproteases. They are expressed by GRANULOCYTES; MONOCYTES; and their precursors as well as by various non-hematopoietic cells. They release an N-terminal amino acid from a peptide, amide or arylamide.
Any part or derivative of any protozoan that elicits immunity; malaria (Plasmodium) and trypanosome antigens are presently the most frequently encountered.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
A costimulatory ligand expressed by ANTIGEN-PRESENTING CELLS that binds to CD28 ANTIGEN with high specificity and to CTLA-4 ANTIGEN with low specificity. The interaction of CD86 with CD28 ANTIGEN provides a stimulatory signal to T-LYMPHOCYTES, while its interaction with CTLA-4 ANTIGEN may play a role in inducing PERIPHERAL TOLERANCE.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.
A tumor necrosis factor receptor subtype found in a variety of tissues and on activated LYMPHOCYTES. It has specificity for FAS LIGAND and plays a role in regulation of peripheral immune responses and APOPTOSIS. Multiple isoforms of the protein exist due to multiple ALTERNATIVE SPLICING. The activated receptor signals via a conserved death domain that associates with specific TNF RECEPTOR-ASSOCIATED FACTORS in the CYTOPLASM.
Antigens determined by leukocyte loci found on chromosome 6, the major histocompatibility loci in humans. They are polypeptides or glycoproteins found on most nucleated cells and platelets, determine tissue types for transplantation, and are associated with certain diseases.
Membrane antigens associated with maturation stages of B-lymphocytes, often expressed in tumors of B-cell origin.
High-molecular weight glycoproteins uniquely expressed on the surface of LEUKOCYTES and their hemopoietic progenitors. They contain a cytoplasmic protein tyrosine phosphatase activity which plays a role in intracellular signaling from the CELL SURFACE RECEPTORS. The CD45 antigens occur as multiple isoforms that result from alternative mRNA splicing and differential usage of three exons.
Process of classifying cells of the immune system based on structural and functional differences. The process is commonly used to analyze and sort T-lymphocytes into subsets based on CD antigens by the technique of flow cytometry.
Substances of fungal origin that have antigenic activity.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The major group of transplantation antigens in the mouse.
A 67-kDa sialic acid binding lectin that is specific for MYELOID CELLS and MONOCYTE-MACROPHAGE PRECURSOR CELLS. This protein is the smallest siglec subtype and contains a single immunoglobulin C2-set domain. It may play a role in intracellular signaling via its interaction with SHP-1 PROTEIN-TYROSINE PHOSPHATASE and SHP-2 PROTEIN-TYROSINE PHOSPHATASE.
Any part or derivative of a helminth that elicits an immune reaction. The most commonly seen helminth antigens are those of the schistosomes.
Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.
Cell-surface glycoprotein beta-chains that are non-covalently linked to specific alpha-chains of the CD11 family of leukocyte-adhesion molecules (RECEPTORS, LEUKOCYTE-ADHESION). A defect in the gene encoding CD18 causes LEUKOCYTE-ADHESION DEFICIENCY SYNDROME.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
A member of the tumor necrosis factor receptor superfamily that may play a role in the regulation of NF-KAPPA B and APOPTOSIS. They are found on activated T-LYMPHOCYTES; B-LYMPHOCYTES; NEUTROPHILS; EOSINOPHILS; MAST CELLS and NK CELLS. Overexpression of CD30 antigen in hematopoietic malignancies make the antigen clinically useful as a biological tumor marker. Signaling of the receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
Glycoproteins found on the membrane or surface of cells.
A critical subpopulation of regulatory T-lymphocytes involved in MHC Class I-restricted interactions. They include both cytotoxic T-lymphocytes (T-LYMPHOCYTES, CYTOTOXIC) and CD8+ suppressor T-lymphocytes.
Sites on an antigen that interact with specific antibodies.
A subtype of tetraspanin proteins that play a role in cell adhesion, cell motility, and tumor metastasis. CD9 antigens take part in the process of platelet activation and aggregation, the formation of paranodal junctions in neuronal tissue, and the fusion of sperm with egg.
A glycoprotein that is secreted into the luminal surface of the epithelia in the gastrointestinal tract. It is found in the feces and pancreaticobiliary secretions and is used to monitor the response to colon cancer treatment.
A subclass of HLA-D antigens that consist of alpha and beta chains. The inheritance of HLA-DR antigens differs from that of the HLA-DQ ANTIGENS and HLA-DP ANTIGENS.
A trisaccharide antigen expressed on glycolipids and many cell-surface glycoproteins. In the blood the antigen is found on the surface of NEUTROPHILS; EOSINOPHILS; and MONOCYTES. In addition, CD15 antigen is a stage-specific embryonic antigen.
Those proteins recognized by antibodies from serum of animals bearing tumors induced by viruses; these proteins are presumably coded for by the nucleic acids of the same viruses that caused the neoplastic transformation.
Established cell cultures that have the potential to propagate indefinitely.
A sialic acid-rich protein and an integral cell membrane mucin. It plays an important role in activation of T-LYMPHOCYTES.
Leukocyte differentiation antigens and major platelet membrane glycoproteins present on MONOCYTES; ENDOTHELIAL CELLS; PLATELETS; and mammary EPITHELIAL CELLS. They play major roles in CELL ADHESION; SIGNAL TRANSDUCTION; and regulation of angiogenesis. CD36 is a receptor for THROMBOSPONDINS and can act as a scavenger receptor that recognizes and transports oxidized LIPOPROTEINS and FATTY ACIDS.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A group of three different alpha chains (CD11a, CD11b, CD11c) that are associated with an invariant CD18 beta chain (ANTIGENS, CD18). The three resulting leukocyte-adhesion molecules (RECEPTORS, LEUKOCYTE ADHESION) are LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN-1; MACROPHAGE-1 ANTIGEN; and ANTIGEN, P150,95.
Large, transmembrane, non-covalently linked glycoproteins (alpha and beta). Both chains can be polymorphic although there is more structural variation in the beta chains. The class II antigens in humans are called HLA-D ANTIGENS and are coded by a gene on chromosome 6. In mice, two genes named IA and IE on chromosome 17 code for the H-2 antigens. The antigens are found on B-lymphocytes, macrophages, epidermal cells, and sperm and are thought to mediate the competence of and cellular cooperation in the immune response. The term IA antigens used to refer only to the proteins encoded by the IA genes in the mouse, but is now used as a generic term for any class II histocompatibility antigen.
A group of antigens that includes both the major and minor histocompatibility antigens. The former are genetically determined by the major histocompatibility complex. They determine tissue type for transplantation and cause allograft rejections. The latter are systems of allelic alloantigens that can cause weak transplant rejection.
Small glycoproteins found on both hematopoietic and non-hematopoietic cells. CD59 restricts the cytolytic activity of homologous complement by binding to C8 and C9 and blocking the assembly of the membrane attack complex. (From Barclay et al., The Leukocyte Antigen FactsBook, 1993, p234)
IMMUNOGLOBULINS on the surface of B-LYMPHOCYTES. Their MESSENGER RNA contains an EXON with a membrane spanning sequence, producing immunoglobulins in the form of type I transmembrane proteins as opposed to secreted immunoglobulins (ANTIBODIES) which do not contain the membrane spanning segment.
Nuclear antigen with a role in DNA synthesis, DNA repair, and cell cycle progression. PCNA is required for the coordinated synthesis of both leading and lagging strands at the replication fork during DNA replication. PCNA expression correlates with the proliferation activity of several malignant and non-malignant cell types.
Oligosaccharide antigenic determinants found principally on NK cells and T-cells. Their role in the immune response is poorly understood.
A transmembrane protein belonging to the tumor necrosis factor superfamily that specifically binds to CD27 ANTIGEN. It is found on activated T-LYMPHOCYTES; B-LYMPHOCYTES; and DENDRITIC CELLS where it plays a role in stimulating the proliferation of CD4-POSITIVE T-LYMPHOCYTES and CD8-POSITIVE T-LYMPHOCYTES.
A ubiquitously expressed complement receptor that binds COMPLEMENT C3B and COMPLEMENT C4B and serves as a cofactor for their inactivation. CD46 also interacts with a wide variety of pathogens and mediates immune response.
A class of animal lectins that bind to carbohydrate in a calcium-dependent manner. They share a common carbohydrate-binding domain that is structurally distinct from other classes of lectins.
Glycoproteins with a wide distribution on hematopoietic and non-hematopoietic cells and strongly expressed on macrophages. CD58 mediates cell adhesion by binding to CD2; (ANTIGENS, CD2); and this enhances antigen-specific T-cell activation.
55-kDa antigens found on HELPER-INDUCER T-LYMPHOCYTES and on a variety of other immune cell types. CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in MAJOR HISTOCOMPATIBILITY COMPLEX class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as INTERLEUKIN-15 receptors and bind to the HIV receptors, binding directly to the HIV ENVELOPE PROTEIN GP120.
A ubiquitously expressed membrane glycoprotein. It interacts with a variety of INTEGRINS and mediates responses to EXTRACELLULAR MATRIX PROTEINS.
A CD antigen that contains a conserved I domain which is involved in ligand binding. When combined with CD18 the two subunits form MACROPHAGE-1 ANTIGEN.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A glycoprotein that is a kallikrein-like serine proteinase and an esterase, produced by epithelial cells of both normal and malignant prostate tissue. It is an important marker for the diagnosis of prostate cancer.
An integrin alpha subunit of approximately 150-kDa molecular weight. It is expressed at high levels on monocytes and combines with CD18 ANTIGEN to form the cell surface receptor INTEGRIN ALPHAXBETA2. The subunit contains a conserved I-domain which is characteristic of several of alpha integrins.
The lipopolysaccharide-protein somatic antigens, usually from gram-negative bacteria, important in the serological classification of enteric bacilli. The O-specific chains determine the specificity of the O antigens of a given serotype. O antigens are the immunodominant part of the lipopolysaccharide molecule in the intact bacterial cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
A specific HLA-A surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-A*02 allele family.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Progenitor cells from which all blood cells derive.
The number of CD4-POSITIVE T-LYMPHOCYTES per unit volume of BLOOD. Determination requires the use of a fluorescence-activated flow cytometer.
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
Carbohydrate antigens expressed by malignant tissue. They are useful as tumor markers and are measured in the serum by means of a radioimmunoassay employing monoclonal antibodies.
GPI-linked membrane proteins broadly distributed among hematopoietic and non-hematopoietic cells. CD55 prevents the assembly of C3 CONVERTASE or accelerates the disassembly of preformed convertase, thus blocking the formation of the membrane attack complex.
Cell adhesion molecules present on virtually all monocytes, platelets, and granulocytes. CD31 is highly expressed on endothelial cells and concentrated at the junctions between them.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Membrane glycoproteins consisting of an alpha subunit and a BETA 2-MICROGLOBULIN beta subunit. In humans, highly polymorphic genes on CHROMOSOME 6 encode the alpha subunits of class I antigens and play an important role in determining the serological specificity of the surface antigen. Class I antigens are found on most nucleated cells and are generally detected by their reactivity with alloantisera. These antigens are recognized during GRAFT REJECTION and restrict cell-mediated lysis of virus-infected cells.
Tetraspanin proteins that are involved in a variety of cellular functions including BASEMENT MEMBRANE assembly, and in the formation of a molecular complexes on the surface of LYMPHOCYTES.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
A member of the tumor necrosis factor receptor superfamily that is specific for 4-1BB LIGAND. It is found in a variety of immune cell types including activated T-LYMPHOCYTES; NATURAL KILLER CELLS; and DENDRITIC CELLS. Activation of the receptor on T-LYMPHOCYTES plays a role in their expansion, production of cytokines and survival. Signaling by the activated receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Proteins prepared by recombinant DNA technology.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.
Polymorphic class I human histocompatibility (HLA) surface antigens present on almost all nucleated cells. At least 20 antigens have been identified which are encoded by the A locus of multiple alleles on chromosome 6. They serve as targets for T-cell cytolytic responses and are involved with acceptance or rejection of tissue/organ grafts.
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).
Receptors present on activated T-LYMPHOCYTES and B-LYMPHOCYTES that are specific for INTERLEUKIN-2 and play an important role in LYMPHOCYTE ACTIVATION. They are heterotrimeric proteins consisting of the INTERLEUKIN-2 RECEPTOR ALPHA SUBUNIT, the INTERLEUKIN-2 RECEPTOR BETA SUBUNIT, and the INTERLEUKIN RECEPTOR COMMON GAMMA-CHAIN.
Sets of cell surface antigens located on BLOOD CELLS. They are usually membrane GLYCOPROTEINS or GLYCOLIPIDS that are antigenically distinguished by their carbohydrate moieties.
Those hepatitis B antigens found on the surface of the Dane particle and on the 20 nm spherical and tubular particles. Several subspecificities of the surface antigen are known. These were formerly called the Australia antigen.
Ubiquitously-expressed tetraspanin proteins that are found in late ENDOSOMES and LYSOSOMES and have been implicated in intracellular transport of proteins.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Tetraspanin proteins found associated with LAMININ-binding INTEGRINS. The CD151 antigens may play a role in the regulation of CELL MOTILITY.
A component of the B-cell antigen receptor that is involved in B-cell antigen receptor heavy chain transport to the PLASMA MEMBRANE. It is expressed almost exclusively in B-LYMPHOCYTES and serves as a useful marker for B-cell NEOPLASMS.
An encapsulated lymphatic organ through which venous blood filters.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Human immune-response or Class II antigens found mainly, but not exclusively, on B-lymphocytes and produced from genes of the HLA-D locus. They are extremely polymorphic families of glycopeptides, each consisting of two chains, alpha and beta. This group of antigens includes the -DR, -DQ and -DP designations, of which HLA-DR is most studied; some of these glycoproteins are associated with certain diseases, possibly of immune etiology.
A membrane-bound tumor necrosis family member found primarily on activated T-LYMPHOCYTES that binds specifically to CD30 ANTIGEN. It may play a role in INFLAMMATION and immune regulation.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
A class of enzymes involved in the hydrolysis of the N-glycosidic bond of nitrogen-linked sugars.
A form of undifferentiated malignant LYMPHOMA usually found in central Africa, but also reported in other parts of the world. It is commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. B-cell antigens are expressed on the immature cells that make up the tumor in virtually all cases of Burkitt lymphoma. The Epstein-Barr virus (HERPESVIRUS 4, HUMAN) has been isolated from Burkitt lymphoma cases in Africa and it is implicated as the causative agent in these cases; however, most non-African cases are EBV-negative.
Molecules on the surface of B- and T-lymphocytes that recognize and combine with specific antigens.
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.
An alpha-integrin subunit found on lymphocytes, granulocytes, macrophages and monocytes. It combines with the integrin beta2 subunit (CD18 ANTIGEN) to form LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN-1.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Antigens of the virion of the HEPATITIS B VIRUS or the Dane particle, its surface (HEPATITIS B SURFACE ANTIGENS), core (HEPATITIS B CORE ANTIGENS), and other associated antigens, including the HEPATITIS B E ANTIGENS.
The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.
The processes triggered by interactions of ANTIBODIES with their ANTIGENS.
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
Mice homozygous for the mutant autosomal recessive gene "scid" which is located on the centromeric end of chromosome 16. These mice lack mature, functional lymphocytes and are thus highly susceptible to lethal opportunistic infections if not chronically treated with antibiotics. The lack of B- and T-cell immunity resembles severe combined immunodeficiency (SCID) syndrome in human infants. SCID mice are useful as animal models since they are receptive to implantation of a human immune system producing SCID-human (SCID-hu) hematochimeric mice.
Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
A heterogeneous group of immunocompetent cells that mediate the cellular immune response by processing and presenting antigens to the T-cells. Traditional antigen-presenting cells include MACROPHAGES; DENDRITIC CELLS; LANGERHANS CELLS; and B-LYMPHOCYTES. FOLLICULAR DENDRITIC CELLS are not traditional antigen-presenting cells, but because they hold antigen on their cell surface in the form of IMMUNE COMPLEXES for B-cell recognition they are considered so by some authors.
The type species of LYMPHOCRYPTOVIRUS, subfamily GAMMAHERPESVIRINAE, infecting B-cells in humans. It is thought to be the causative agent of INFECTIOUS MONONUCLEOSIS and is strongly associated with oral hairy leukoplakia (LEUKOPLAKIA, HAIRY;), BURKITT LYMPHOMA; and other malignancies.
T-cell receptors composed of CD3-associated alpha and beta polypeptide chains and expressed primarily in CD4+ or CD8+ T-cells. Unlike immunoglobulins, the alpha-beta T-cell receptors recognize antigens only when presented in association with major histocompatibility (MHC) molecules.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Class I human histocompatibility (HLA) surface antigens encoded by more than 30 detectable alleles on locus B of the HLA complex, the most polymorphic of all the HLA specificities. Several of these antigens (e.g., HLA-B27, -B7, -B8) are strongly associated with predisposition to rheumatoid and other autoimmune disorders. Like other class I HLA determinants, they are involved in the cellular immune reactivity of cytolytic T lymphocytes.
The altered state of immunologic responsiveness resulting from initial contact with antigen, which enables the individual to produce antibodies more rapidly and in greater quantity in response to secondary antigenic stimulus.
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
The phenomenon of target cell destruction by immunologically active effector cells. It may be brought about directly by sensitized T-lymphocytes or by lymphoid or myeloid "killer" cells, or it may be mediated by cytotoxic antibody, cytotoxic factor released by lymphoid cells, or complement.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
A melanosome-specific protein that plays a role in the expression, stability, trafficking, and processing of GP100 MELANOMA ANTIGEN, which is critical to the formation of Stage II MELANOSOMES. The protein is used as an antigen marker for MELANOMA cells.
A widely distributed cell surface transmembrane glycoprotein that stimulates the synthesis of MATRIX METALLOPROTEINASES. It is found at high levels on the surface of malignant NEOPLASMS and may play a role as a mediator of malignant cell behavior.
A general term for various neoplastic diseases of the lymphoid tissue.
An albumin obtained from the white of eggs. It is a member of the serpin superfamily.
Antigens associated with specific proteins of the human adult T-cell immunodeficiency virus (HIV); also called HTLV-III-associated and lymphadenopathy-associated virus (LAV) antigens.
An inhibitory T CELL receptor that is closely related to CD28 ANTIGEN. It has specificity for CD80 ANTIGEN and CD86 ANTIGEN and acts as a negative regulator of peripheral T cell function. CTLA-4 antigen is believed to play role in inducing PERIPHERAL TOLERANCE.
A promyelocytic cell line derived from a patient with ACUTE PROMYELOCYTIC LEUKEMIA. HL-60 cells lack specific markers for LYMPHOID CELLS but express surface receptors for FC FRAGMENTS and COMPLEMENT SYSTEM PROTEINS. They also exhibit phagocytic activity and responsiveness to chemotactic stimuli. (From Hay et al., American Type Culture Collection, 7th ed, pp127-8)
A widely expressed transmembrane glycoprotein that functions as a METASTASIS suppressor protein. It is underexpressed in a variety of human NEOPLASMS.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
A group of differentiation surface antigens, among the first to be discovered on thymocytes and T-lymphocytes. Originally identified in the mouse, they are also found in other species including humans, and are expressed on brain neurons and other cells.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
The specific failure of a normally responsive individual to make an immune response to a known antigen. It results from previous contact with the antigen by an immunologically immature individual (fetus or neonate) or by an adult exposed to extreme high-dose or low-dose antigen, or by exposure to radiation, antimetabolites, antilymphocytic serum, etc.
Manifestations of the immune response which are mediated by antigen-sensitized T-lymphocytes via lymphokines or direct cytotoxicity. This takes place in the absence of circulating antibody or where antibody plays a subordinate role.
A single, unpaired primary lymphoid organ situated in the MEDIASTINUM, extending superiorly into the neck to the lower edge of the THYROID GLAND and inferiorly to the fourth costal cartilage. It is necessary for normal development of immunologic function early in life. By puberty, it begins to involute and much of the tissue is replaced by fat.
Endogenous tissue constituents that have the ability to interact with AUTOANTIBODIES and cause an immune response.
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
Nuclear antigens encoded by VIRAL GENES found in HUMAN HERPESVIRUS 4. At least six nuclear antigens have been identified.
A soluble substance elaborated by antigen- or mitogen-stimulated T-LYMPHOCYTES which induces DNA synthesis in naive lymphocytes.
A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.
A cell line derived from cultured tumor cells.
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
A sex-specific cell surface antigen produced by the sex-determining gene of the Y chromosome in mammals. It causes syngeneic grafts from males to females to be rejected and interacts with somatic elements of the embryologic undifferentiated gonad to produce testicular organogenesis.
A cell adhesion molecule of the immunoglobulin superfamily that is expressed in ENDOTHELIAL CELLS and is involved in INTERCELLULAR JUNCTIONS.
Antigens stimulating the formation of, or combining with heterophile antibodies. They are cross-reacting antigens found in phylogenetically unrelated species.
CD4-positive T cells that inhibit immunopathology or autoimmune disease in vivo. They inhibit the immune response by influencing the activity of other cell types. Regulatory T-cells include naturally occurring CD4+CD25+ cells, IL-10 secreting Tr1 cells, and Th3 cells.
Antibodies obtained from a single clone of cells grown in mice or rats.
Antigenic determinants recognized and bound by the T-cell receptor. Epitopes recognized by the T-cell receptor are often located in the inner, unexposed side of the antigen, and become accessible to the T-cell receptors after proteolytic processing of the antigen.
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
A heterodimeric protein that is a cell surface antigen associated with lymphocyte activation. The initial characterization of this protein revealed one identifiable heavy chain (ANTIGENS, CD98 HEAVY CHAIN) and an indeterminate smaller light chain. It is now known that a variety of light chain subunits (ANTIGENS, CD98 LIGHT CHAINS) can dimerize with the heavy chain. Depending upon its light chain composition a diverse array of functions can be found for this protein. Functions include: type L amino acid transport, type y+L amino acid transport and regulation of cellular fusion.
The hepatitis B antigen within the core of the Dane particle, the infectious hepatitis virion.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
The complex formed by the binding of antigen and antibody molecules. The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES.
They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system.
The sum of the weight of all the atoms in a molecule.
Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.
A group of the D-related HLA antigens found to differ from the DR antigens in genetic locus and therefore inheritance. These antigens are polymorphic glycoproteins comprising alpha and beta chains and are found on lymphoid and other cells, often associated with certain diseases.
Immunoglobulins produced in response to VIRAL ANTIGENS.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
A glycolipid, cross-species antigen that induces production of antisheep hemolysin. It is present on the tissue cells of many species but absent in humans. It is found in many infectious agents.
Elements of limited time intervals, contributing to particular results or situations.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
An inhibitory B7 antigen that has specificity for the T-CELL receptor PROGRAMMED CELL DEATH 1 PROTEIN. CD274 antigen provides negative signals that control and inhibit T-cell responses and is found at higher than normal levels on tumor cells, suggesting its potential role in TUMOR IMMUNE EVASION.
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
A species of POLYOMAVIRUS originally isolated from Rhesus monkey kidney tissue. It produces malignancy in human and newborn hamster kidney cell cultures.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
Substances that augment, stimulate, activate, potentiate, or modulate the immune response at either the cellular or humoral level. The classical agents (Freund's adjuvant, BCG, Corynebacterium parvum, et al.) contain bacterial antigens. Some are endogenous (e.g., histamine, interferon, transfer factor, tuftsin, interleukin-1). Their mode of action is either non-specific, resulting in increased immune responsiveness to a wide variety of antigens, or antigen-specific, i.e., affecting a restricted type of immune response to a narrow group of antigens. The therapeutic efficacy of many biological response modifiers is related to their antigen-specific immunoadjuvanticity.
Antigens that exist in alternative (allelic) forms in a single species. When an isoantigen is encountered by species members who lack it, an immune response is induced. Typical isoantigens are the BLOOD GROUP ANTIGENS.
Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell.
A melanosome-associated protein that plays a role in the maturation of the MELANOSOME.
The genetic region which contains the loci of genes which determine the structure of the serologically defined (SD) and lymphocyte-defined (LD) TRANSPLANTATION ANTIGENS, genes which control the structure of the IMMUNE RESPONSE-ASSOCIATED ANTIGENS, HUMAN; the IMMUNE RESPONSE GENES which control the ability of an animal to respond immunologically to antigenic stimuli, and genes which determine the structure and/or level of the first four components of complement.
Bone marrow-derived lymphocytes that possess cytotoxic properties, classically directed against transformed and virus-infected cells. Unlike T CELLS; and B CELLS; NK CELLS are not antigen specific. The cytotoxicity of natural killer cells is determined by the collective signaling of an array of inhibitory and stimulatory CELL SURFACE RECEPTORS. A subset of T-LYMPHOCYTES referred to as NATURAL KILLER T CELLS shares some of the properties of this cell type.
A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
Form of passive immunization where previously sensitized immunologic agents (cells or serum) are transferred to non-immune recipients. When transfer of cells is used as a therapy for the treatment of neoplasms, it is called adoptive immunotherapy (IMMUNOTHERAPY, ADOPTIVE).

CD5 negatively regulates the T-cell antigen receptor signal transduction pathway: involvement of SH2-containing phosphotyrosine phosphatase SHP-1. (1/601)

The negative regulation of T- or B-cell antigen receptor signaling by CD5 was proposed based on studies of thymocytes and peritoneal B-1a cells from CD5-deficient mice. Here, we show that CD5 is constitutively associated with phosphotyrosine phosphatase activity in Jurkat T cells. CD5 was found associated with the Src homology 2 (SH2) domain containing hematopoietic phosphotyrosine phosphatase SHP-1 in both Jurkat cells and normal phytohemagglutinin-expanded T lymphoblasts. This interaction was increased upon T-cell receptor (TCR)-CD3 cell stimulation. CD5 co-cross-linking with the TCR-CD3 complex down-regulated the TCR-CD3-increased Ca2+ mobilization in Jurkat cells. In addition, stimulation of Jurkat cells or normal phytohemagglutinin-expanded T lymphoblasts through TCR-CD3 induced rapid tyrosine phosphorylation of several protein substrates, which was substantially diminished after CD5 cross-linking. The CD5-regulated substrates included CD3zeta, ZAP-70, Syk, and phospholipase Cgammal but not the Src family tyrosine kinase p56(lck). By mutation of all four CD5 intracellular tyrosine residues to phenylalanine, we found the membrane-proximal tyrosine at position 378, which is located in an immunoreceptor tyrosine-based inhibitory (ITIM)-like motif, crucial for SHP-1 association. The F378 point mutation ablated both SHP-1 binding and the down-regulating activity of CD5 during TCR-CD3 stimulation. These results suggest a critical role of the CD5 ITIM-like motif, which by binding to SHP-1 mediates the down-regulatory activity of this receptor.  (+info)

An alternatively spliced form of CD79b gene may account for altered B-cell receptor expression in B-chronic lymphocytic leukemia. (2/601)

Several functional anomalies of B-chronic lymphocytic leukemia (B-CLL) cells may be explained by abnormalities of the B-cell receptor (BCR), a multimeric complex formed by the sIg homodimer and the noncovalently bound heterodimer Igalpha/Igbeta (CD79a/CD79b). Because the expression of the extracellular Ig-like domain of CD79b has been reported to be absent in the cells of most CLL cases, we have investigated the molecular mechanisms that may account for this defect. Peripheral blood lymphocytes (PBL) from 50 patients and two cell lines (MEC1, MEC2) obtained from the PBL of one of them were studied. MEC1, MEC2, and 75% of CLL cases did not express detectable levels of the extracellular Ig-like domain of CD79b, which was nevertheless present in greater than 80% CD19(+) cells from normal donors. In healthy subjects the expression of CD79b was equally distributed in CD5(+) and CD5(-) B-cell subsets. Reverse transcription-polymerase chain reaction (RT-PCR) analysis of CD79b RNA from all patients and from MEC1 and MEC2 cell lines consistently yielded two fragments of different size (709 bp and 397 bp). The 709-bp band corresponds to CD79b entire transcript; the 397-bp band corresponds to an alternatively spliced form lacking exon 3 that encodes the extracellular Ig-like domain. Both fragments were also visible in normal PBL. The expression of the 397-bp fragment was increased in normal activated B cells, while no difference was seen between CD5(+) and CD5(-) B cells. To obtain a more accurate estimate of the relative proportions of the two spliced forms, a radioactive PCR was performed in 13 normal and 22 B-CLL samples and the results analyzed using a digital imager. The mean value of the CD79b to the CD79b internally deleted ratio was 0.64 +/- 0.20 SD in normal donors and 0.44 +/- 0.27 SD in B-CLL (P =.01). Direct sequencing of 397-bp RT-PCR products and of genomic DNA corresponding to exon 3 from MEC1, MEC2, their parental cells, and five fresh B-CLL samples did not show any causal mutation. Single-strand conformation polymorphism analysis of exon 3 performed in 18 additional B-CLL cases showed a single abnormal shift corresponding to a TGT --> TGC polymorphic change at amino acid 122. We propose a role for the alternative splicing of CD79b gene in causing the reduced expression of BCR on the surface of B-CLL cells. As normal B cells also present this variant, the mechanism of CD79b posttranscriptional regulation might reflect the activation stage of the normal B cell from which B-CLL derives.  (+info)

Experimental autoimmune myasthenia gravis and CD5+ B-lymphocyte expression. (3/601)

Myasthenia gravis is one of the typical organ specific autoimmune disease and the CD5+ B-lymphocytes are known to be associated with the secretion of autoimmune antibodies. The authors performed the study to establish an animal model of experimental autoimmune myasthenia gravis (EAMG) by immunizing the nicotinic acetylcholine receptor (AChR) and to understand CD5+ B-lymphocyte changes in peripheral blood of EAMGs. Lewis rats weighing 150-200 g were injected subcutaneously three times with 50 microg AChR purified from the electric organ of Torpedo marmorata and Freund's adjuvant. The EAMG induction was assessed by evaluating clinical manifestations. The CD5+ B-lymphocyte was double stained using monoclonal PE conjugated anti-CD5+ and FITC conjugated anti-rat CD45R antibodies and calculated using a fluorescence-activated cell sorter (FACS). In three out of ten Lewis rats injected with purified AChR, the EAMG models were established. The animals showed definite clinical weakness responded to neostigmine; they had difficulty in climbing the slope, or easily fell down from a vertical cage. The range of CD5+ B-lymphocytes of peripheral blood in the EAMG models was 10.2%-17.5%, which was higher than in controls. In conclusion, the EAMG models were successfully established and the CD5+ B-lymphocyte expression in peripheral blood increased in EAMGs. This provided indirect evidence of the autoimmune pathomechanism of human myasthenia gravis.  (+info)

CD5 positive breast carcinoma in a patient with untreated chronic lymphocytic leukaemia: molecular studies of chromosome 13q. (4/601)

A 67 year old woman presented with a right breast lump which proved to be a grade 2 invasive ductal carcinoma with axillary lymph node metastasis. She had a five year history of CD5 positive chronic lymphocytic leukaemia, which never required treatment. Immunoperoxidase stains for CD5, using the monoclonal antibody NCL-CD-54C7, showed that there was extensive infiltration of axillary lymph nodes with CD5 positive B lymphocytes. Strong staining for CD5 was also seen in the carcinoma cells within the breast and lymph node metastases. It has recently been suggested that there is a tumour suppresser locus in chronic lymphocytic leukaemia at 13q12.3 near or at the BRCA2 locus. Deletion of regions on chromosome 13q containing the BRCA2 and RB1 genes has also been reported in sporadic breast cancers. These observations suggest that there may be a link between these two diseases acting through chromosome 13, but amplification of several microsatellite repeat markers failed to show any loss of heterozygosity or repeat instability at either these or several other loci on chromosome 13. Examination of additional such cases is needed to perform a more comprehensive study of the significance of positive CD5 staining of breast carcinoma.  (+info)

Anti-phospholipid antibodies and CD5+ B cells in HIV infection. (5/601)

This cross-sectional study evaluates the correlation between anti-phospholipid antibodies and CD5+ B cells in 110 patients infected with HIV-1. There were 89.1% of the patients who had IgG antibodies against cardiolipin and phosphatidylserine. The prevalence of IgM and IgA antibodies was < 22%. AIDS was associated with lower frequencies of IgM antibodies against cardiolipin (P = 0.05) and IgG-antibodies against cardiolipin and phosphatidylserine (P = 0.011). Drug users had higher IgM antibodies against phospholipids than patients from other risk groups (P = 0.02). A history of thromboembolic events was not accompanied by higher levels of anti-phospholipid antibodies (P > 0.2). No correlation between anti-phospholipid antibodies and CD5+ B cells was detected. Percentage part of CD5+ B lymphocytes was elevated in all patients and absolute CD4+ T lymphocyte counts and HIV p24 antigen were inversely correlated. In advanced disease a significant reduction of anti-phospholipid antibodies was contrasted with persistent elevation of CD5+ B lymphocytes. These observations may reflect immunological dysfunction involving apoptosis and endothelial damage rather than polyclonal B cell hyperstimulation. A possible explanation would be that in HIV infection an increased rate of spontaneous apoptosis in peripheral blood lymphocytes is accompanied by functional and structural changes of mitochondria. Therefore, structurally altered mitochondrial phospholipids could serve as antigen to induce specific humoral immune responses.  (+info)

Positive selection as a developmental progression initiated by alpha beta TCR signals that fix TCR specificity prior to lineage commitment. (6/601)

During positive selection, immature thymocytes commit to either the CD4+ or CD8+ T cell lineage ("commitment") and convert from short-lived thymocytes into long-lived T cells ("rescue"). By formal precursor-progeny analysis, we now identify what is likely to be the initial positive selection step signaled by alpha beta TCR, which we have termed "induction". During induction, RAG mRNA expression is downregulated, but lineage commitment does not occur. Rather, lineage commitment (which depends upon the MHC class specificity of the alpha beta TCR) only occurs after downregulation of RAG expression and the consequent fixation of alpha beta TCR specificity. We propose that positive selection can be viewed as a sequence of increasingly selective developmental steps (induction-->commitment-->rescue) that are signaled by alpha beta TCR engagements of intrathymic ligands.  (+info)

Signaling through CD5 involves acidic sphingomyelinase, protein kinase C-zeta, mitogen-activated protein kinase kinase, and c-Jun NH2-terminal kinase. (7/601)

The CD5 lymphocyte surface glycoprotein is a coreceptor involved in the modulation of Ag-specific receptor-mediated activation and differentiation signals. The molecular basis for its modulatory properties is not yet well understood. In the present study we describe early biochemical events triggered by CD5 stimulation, which include the phosphatidylcholine-specific phospholipase C (PC-PLC)-dependent activation of acidic sphingomyelinase (A-SMase) in normal and lymphoblastoid T and B cells. The functional coupling of PC-PLC and A-SMase is demonstrated by the abrogation of A-SMase activation by 1) xanthogenate tricyclodecan-9-yl (D609), a selective inhibitor of PC-PLC, and 2) replacement of several C-terminal serine residues (S458, S459, and S461) present in the cytoplasmic tail of CD5 that are known to be critical for PC-PLC activation. Additionally, we demonstrate that activation of protein kinase C-zeta (PKC-zeta) and members of the mitogen-activated protein kinase (MAPK) cascade (MAPK kinase and c-Jun NH2-terminal kinase), but not the NF-kappaB, are downstream events of the CD5 signaling pathway. A-SMase, PKC-zeta, and MAPK family members are key mediators of cell responses as diverse as proliferation, differentiation, and growth arrest and may contribute to CD5-mediated modulation of TCR or BCR signaling.  (+info)

ChT1, an Ig superfamily molecule required for T cell differentiation. (8/601)

The thymus is colonized by circulating progenitor cells that differentiate into mature T cells under the influence of the thymic microenvironment. We report here the cloning and function of the avian thymocyte Ag ChT1, a member of the Ig superfamily with one V-like and one C2-like domain. ChT1-positive embryonic bone marrow cells coexpressing c-kit give rise to mature T cells upon intrathymic cell transfer. ChT1-specific Ab inhibits T cell differentiation in embryonic thymic organ cultures and in thymocyte precursor cocultures on stromal cells. Thus, we provide clear evidence that ChT1 is a novel Ag on early T cell progenitors that plays an important role in the early stages of T cell development.  (+info)

How to treat a patient with c-Myc positive diffuse large B-Cell lymphoma? - From Our Readers, Viewpoints, You Make the Call: Readers Response - ASH Clinical News
Study Flashcards On chronic lymphocytic leukemias at Cram.com. Quickly memorize the terms, phrases and much more. Cram.com makes it easy to get the grade you want!
CD20/CD22-positive diffuse large B-cell NHL that has relapsed after 1 or 2 prior therapies; one prior therapy must include anthracyclines and one must include rituximab in combination with chemotherapy. - Relapsed/disease progression within 12 months after start of prior therapy and/or secondary International Prognostic Index (sIPI) score greater than 1. - Eligible for autologous stem cell transplant (aSCT). ...
This dissertation presents my studies investigating the delivery of CD40 ligand (CD40L, CD154) from helper T lymphocytes to antigen-presenting B lymphocytes. CD40L is a type two transmembrane TNF superfamily cytokine made by T cells that engages CD40 on antigen-presenting cells, including B cells, initiating downstream signaling resulting in B cell proliferation, differentiation, and antibody formation. Helper T cells can produce CD40L de novo upon antigen-specific interactions, but they also have an intracellular secretory compartment containing a small amount of preformed CD40L that is brought to the T cell surface rapidly upon antigen recognition. In the second chapter of the dissertation, I investigate the function of preformed CD40L in the absence of de novo CD40L. Preformed CD40L is capable of fulfilling some functions previously assumed to require de novo CD40L, including upregulation of costimulatory molecules, production of cytokines by DCs, and antigen-specific proliferation of B cells.
Genetic factors that contribute to increased incidence of CLL:. - Early studies have associated the risk of developing aggressive CLL with polymorphisms in the gene encoding CD5 (located at 11q13), CD38 (located at 4p15), or tumor necrosis factor-α (TNF-α) and other genes mapping to 13q21.33-q22.2.. - Disease-susceptibility associated with single nucleotide polymorphisms in or around genes encoding proteins involved in apoptosis or immune regulatory pathways, namely CCNH (located at 5q13.3), APAF1 (located at 12q23), IL16 (located at 15q26.3), CASP8 (located at 2q33.1), NOS2A (located at 17q11.1), and CCR7 (located at 17q21.2). ...
Aggressive CD20 positive Diffuse Large B-cell lymphoma confirmed by Laboratory of Pathology, NCI.. HIV + serology.. All stages (I-IV) of disease.. ECOG Performance status 0-4. NHL previously untreated with cytotoxic chemotherapy; however, patients may be entered if they have had prior cyclophosphamide for an urgent problem at diagnosis (e.g. epidural cord compression, superior vena cava syndrome) and/or a single dose of intrathecal methotrexte (MTX) at the time of the pre-treatment diagnostic lumbar puncture. Age greater than or equal to 18 years. Laboratory tests (unless impairment due to respective organ involvement by tumor):. ...
Aggressive CD20 positive Diffuse Large B-cell lymphoma confirmed by Laboratory of Pathology, NCI.. HIV + serology.. All stages (I-IV) of disease.. ECOG Performance status 0-4. NHL previously untreated with cytotoxic chemotherapy; however, patients may be entered if they have had prior cyclophosphamide for an urgent problem at diagnosis (e.g. epidural cord compression, superior vena cava syndrome) and/or a single dose of intrathecal methotrexte (MTX) at the time of the pre-treatment diagnostic lumbar puncture. Age greater than or equal to 18 years. Laboratory tests (unless impairment due to respective organ involvement by tumor):. ...
We recently demonstrated that the prognosis for de novo CD5-positive (CD5+) diffuse large-B-cell lymphoma (DLBCL) is markedly worse than that for CD5-negative (CD5-) DLBCL. Our findings also suggested that on the basis of its clinical features CD5+ DLBCL may constitute a unique disease category. How …
In the past decade, the suppressive effects, mainly through the secretion of IL-10, of regulatory B cells on inflammatory responses have been reported in a variety of immune disorders (33-36). Additionally, immune regulation through the interaction of immune cells with the intrinsic phenotype of regulatory B cells (e.g., CD1dhiCD5+, T2-MZP, Tim-1+, and CD9+) were demonstrated in various diseases, and it plays a critical role in autoimmune diseases (37). In recent studies, functional studies in cancer diseases are emerging (38-40). In particular, the change of the distribution of regulatory B cells in cancer tissue is considered to one of important indicators (8-10). Emerging evidence suggests that regulatory B cells suppress effector immune cells including IFN-γ-producing cytotoxicity cells in various cancer diseases through the secretion of IL-10 (11). Although regulatory B cells have to play the suppressive role on the effector function of T cells in autoimmune diseases to cure diseases (41), ...
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The described experiments indicate that a relatively simple procedure of delivering peptide for a prolonged time period in subimmunogenic forms is suited to induce de novo CD4+25+ suppressor T cells from naive T cells in peripheral lymphoid organs in the absence of a functioning thymus as well as in the absence of a developing immune response. By all investigated criteria (i.e., surface phenotype, long-term stability in the absence of the inducing TCR ligand, Foxp3 expression, Ag-induced expansion in vivo, and suppressive activity in vitro and in vivo as well as cytokine production), the induced suppressor T cells are indistinguishable from intrathymically generated CD4+25+ T cells that were shown to have an essential role in preventing autoimmunity under physiological conditions.. The described experiments are akin to studies conducted by i.v. injection of soluble proteins that were described decades ago and that became known as low zone tolerance experiments (29, 30). It is well possible ...
The described experiments indicate that a relatively simple procedure of delivering peptide for a prolonged time period in subimmunogenic forms is suited to induce de novo CD4+25+ suppressor T cells from naive T cells in peripheral lymphoid organs in the absence of a functioning thymus as well as in the absence of a developing immune response. By all investigated criteria (i.e., surface phenotype, long-term stability in the absence of the inducing TCR ligand, Foxp3 expression, Ag-induced expansion in vivo, and suppressive activity in vitro and in vivo as well as cytokine production), the induced suppressor T cells are indistinguishable from intrathymically generated CD4+25+ T cells that were shown to have an essential role in preventing autoimmunity under physiological conditions.. The described experiments are akin to studies conducted by i.v. injection of soluble proteins that were described decades ago and that became known as low zone tolerance experiments (29, 30). It is well possible ...
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Anaplastic lymphoma kinase (ALK)-positive diffuse large B-cell lymphoma (DLBCL) is a rare, recently defined tumor distinct in many aspects from ALK-positive anaplastic large cell lymphoma (ALCL). We present two additional cases of ALK+DLBCL recently diagnosed in our department and a review of literature. A 48-year old man presented with a large upper neck mass growing slowly over 18 months. Histologically the tumor was diagnosed as an ALK-positive diffuse large B-cell lymphoma. with plasmablastic features. Large, frequently intrasinusoidal tumor cells expressed CD138, EMA, weakly IgA and kappa, but were negative for other B-cell markers, T-cell markers and CD30. The ALK staining was cytoplasmic with the increased intensity in the Golgi area. At the diagnosis the patient manifested with the stage IIIB. Three courses of CHOP resulted in partial and only transient remission. The patient died of massive bleeding from his decomposing tumor 3 months after the diagnosis. A 49-year old man complaining ...
Fibrin associated Epstein-Barr Virus (EBV)-positive Diffuse Large B-Cell Lymphoma (DLBCL) - a published case study within a frontotemporal arachnoid cyst with hemorrhage
To determine the clinical significance of CD5 expression in diffuse large B-cell lymphoma (DLBL) without a clinical history of low-grade B-cell lymphoma, we have reviewed the clinical features and therapeutic outcome of 25 patients with de novo CD5-positive DLBL, and compared the results with those of 87 patients with CD5-negative ...
Etiologic-based therapy is an ideal pharmacological option to treat or prevent diseases. There is no known etiology for multiple sclerosis (MS); however, envir...
Comentário: Bom aí está o novo CD do Ozzy, com uma pegada diferente de Black Rain muitos poderão não gostar e muitos vão amar, mas na minha opinião esse álbum tá bem ao estilo Ozzy só que diferente devido as pitadas de Firewind que Gus G. deixou nas bases e nos solos de guitarra. Recomendo que baixe pois na minha opinião ficou bom, é tipo um daqueles álbuns que não pode faltar quando você estiver na estrada! ...
It is generally assumed that chronic lymphocytic leukemia of B cell origin (B-CLL) is characterized by the presence of surface membrane immunoglobulins (SmIg) and by the absence of cytoplasmic immunoglobulins (CyIg). In a variable number of cases SmI
A variety of cell surface markers have been proposed for different regulatory B cell subsets (21, 22). The generalized ex vivo phenotype of B10 cells from untreated mice is IgMhighIgDlowCD1dhighCD5+CD19highCD23lowB220high, with ,10% coexpressing IgG or IgA (13, 15, 35, 38). Thereby, spleen B10 cells share surface markers with multiple phenotypically defined B cell subsets, including transitional, marginal zone, marginal zone precursor, memory, and B1 B cells (6, 11, 13-15, 38, 41, 43, 44). Spleen B10 cells are enriched within the CD1dhighCD5+CD19high subpopulation (Fig. 2), where 15-20% are B10 cells, and up to 50% are B10+B10pro cells (6, 13, 15, 29). Small numbers of B10 cells are also found within other spleen B cell fractions. The phenotype of B10pro cells after culture reflects their in vitro activation more than their subset of origin. For example, most mouse and human B cells upregulate CD5 expression following CD40 stimulation in vitro (31, 32). Spleen IL-10+ B cells are also enriched ...
We present a case of a female patient (79 years) with pathohistologic diagnosis of Hodgkins lymphoma (HL) (stage IIIB, histologic type MC) for which she was treated with chemotherapy according to LVPP protocol (6 cycles) with good therapeutic response. Unexpectedly, 18 months after HL diagnosis leukocytosis occurred (19.4 x 10(9)/L) with 65% of lymphocytes with lymphoplasmocytic differentiation. Immunophenotype of these cells is typical for B-chronic lymphocytic leukemia (B-CLL) (CD5/CD19+, CD23-, CD38 +/-; with weak expression of monoclonal light chains lambda). Molecular analysis confirmed clonal immunoglobulin heavy chain gene (IgH) rearrangement of peripheral blood lymphocytes. The diagnosis of B-CLL imposed the question of the connection between two neoplasms of lymphocytic origin. Molecular analysis of lymph node biopsy taken at the time of lymphoma diagnosis revealed clonal population of B lymphocytes. That test undeniably proved coexistence of both diseases from the beginning. The latest PCR
CD 11c or Integrin alpha-X (ITGAX) is a heterodimeric glycoprotein consisting of an α- and β-subunit and has seven repeating integrin domains. This transmembrane receptor type I plays a pivotal role in T cell killing and mediates intercellular adhesions during inflammation. Predominat expression levels have been found in dendritic cells, monocytes, macrophages, neutrophils and a small subset of B cells. Under pathological conditions, CD 11c is a marker for hairy cell leukemia, acute non-lymphocytic leukemias, and some chronic lymphocytic leukemias ...
B cell subsets have been found to exhibit a negative regulatory function, like Tregs. The present study investigates the effects of CD5+CD19+ interleukin (IL)-10 (B10) on the occurrence and development of oesophageal carcinoma by analysing B10 levels in the peripheral blood of patients with...
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IL-21 can induce both plasma cells and regulatory B cells. In this article, we demonstrate that untreated HIV patients display CD4+ T cells with enhanced IL-21 expression and high in vivo frequencies of regulatory B cells overexpressing the serine protease granzyme B. Granzyme B-expressing regulatory B cells (GraB cells) cells from HIV patients exhibit increased expression of CD5, CD43, CD86, and CD147 but do not produce IL-10. The main functional characteristic of their regulatory activity is direct granzyme B-dependent degradation of the TCR-ζ-chain, resulting in significantly decreased proliferative T cell responses. Although Th cells from HIV patients secrete IL-21 in a Nef-dependent manner, they barely express CD40L. When culturing such IL-21+CD40L− Th cells with B cells, the former directly induce B cell differentiation into GraB cells. In contrast, the addition of soluble CD40L multimers to T cell/B cell cultures redirects B cell differentiation toward plasma cells, indicating that ...
Carole Goutsmedt, Laëtitia Le Pottier, Jacques-Olivier Pers. Identification of an antigen-specific regulatory B cell subset in humans.. 35th European Workshop for Rheumatology Research, Mar 2015, Budapest, Hungary. 74 (Supplément 1 A1.27), pp.A11, 2015, Annals of the Rheumatic Diseases. 〈hal-01128705〉 ...
In this issue of Clinical Cancer Research, Saiya-Cork and colleagues used integrative genomic profiling to identify that the insulin receptor (INSR) is significantly overexpressed in about 25% of chronic lymphocytic leukemias (CLL), many of which carry deletion 11q (1). Deletion 11q has been associated with marked lymphadenopathy and rapid disease progression in CLL, leading to short overall survival (2, 3). At diagnosis or initiation of first therapy, deletion 11q is the most common high-risk abnormality in CLL. Although the molecular pathogenesis of CLL with each characteristic chromosome abnormality is being intensively studied, much remains to be understood. Most interest in 11q deletion has focused on loss of the ataxia telangiectasia mutated (ATM) gene, a well-known tumor suppressor gene involved in cell cycle checkpoint signaling and DNA repair. Because 11q deletion generally only affects one of the two chromosomes, the other ATM allele would be expected to be mutated if ATM is a key ...
Warwick Davis has praised the late Bob Monkhouse for being a genius. The 44-year-old star - who is to follow in Bobs footsteps by taking over as host of game show Celebrity Squares when it re-launches on ITV - has confessed he knows he has big…
Ibrutinib, a new drug under development, is showing promise as a treatment for mantle cell lymphoma and chronic lymphocytic leukemia (CLL), according to new studies.
TLR-9 and IL-15 synergy promotes the in vitro clonal expansion of chronic lymphocytic leukemia B cellsMongini PK, Gupta R, Boyle E, Nieto J, Lee H, Stein J, Bandovic J, Stankovic T, Barrientos J, Kolitz JE, Allen SL, Rai K, Chu CC, Chiorazzi N, J Immunol. 2015 Aug 1; 195(3):901-23. doi: 10.4049/jimmunol.1403189. Epub 2015 Jul 1 ...
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B-cell chronic lymphocytic leukemia (CLL) patients display leukemic clones bearing either germline or somatically mutated immunoglobulin heavy variable (IGHV ) genes. Most information on CLL immunoglobulins (Igs), such as the definition of stereotyped B-cell receptors (BCRs), was derived from germline unmutated Igs. In particular, detailed studies on the distribution and nature of mutations in paired heavy- and light-chain domains of CLL clones bearing mutated Igs are lacking. To address the somatic hyper-mutation dynamics of CLL Igs, we analyzed the mutation pattern of paired IGHV-diversity-joining (IGHV-D-J ) and immunoglobulin kappa/lambda variable-joining (IGK/LV-J ) rearrangements of 193 leukemic clones that displayed ≥ 2% mutations in at least one of the two immunoglobulin variable (IGV ) genes (IGHV and/or IGK/LV ). The relationship between the mutation frequency in IGHV and IGK/LV complementarity determining regions (CDRs) and framework regions (FRs) was evaluated by correlation ...
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Hematology and Immunology. Uppsala University, Science for Life Laboratory, SciLifeLab. ...
Description: B-Cell Chronic Lymphocytic Leukemia - Pipeline Review, H1 2017, provides an overview of the B-Cell Chronic Lymphocytic Leukemia (Infectious Di
Uzonyi, Barbara and Mácsik-Valent, Bernadett and Lukácsi, Szilvia Zsófia and Kiss, Richárd and Török, Katalin and Kremlitzka, Mariann and Bajtay, Zsuzsanna and Demeter, Judit and Bödör, Csaba and Erdei, Anna (2017) Functional studies of chronic lymphocytic leukemia B cells expressing beta2-integrin type complement receptors CR3 and CR4. IMMUNOLOGY LETTERS, 189. pp. 73-81. ISSN 0165-2478 Peti, E. and Schellenberger, Judit and Németh, G. and Málnási Csizmadia, G. and Oláh, I. and Török, Katalin and Czóbel, Szilárd (2017) Presentation of the HUSEEDwild - a seed weight and germination database of the Pannonian flora - through analysing life forms and social behaviour types. APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH, 15 (1). pp. 225-244. ISSN 1589-1623 Kövendi-Jakó, Anna and Csecserits, Anikó and Halassy, Melinda and Halász, Krisztián and Szitár, Katalin and Török, Katalin (2017) Relationship of germination and establishment for twelve plant species in restored dry grassland. ...
Fingerprint Dive into the research topics of Cytokine gene expression in B-cell chronic lymphocytic leukemia: Evidence of constitutive interleukin-8 (IL-8) mRNA expression and secretion of biologically active IL-8 protein. Together they form a unique fingerprint. ...
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CD22 expression mediates the regulatory functions of peritoneal B-1a cells during the remission phase of contact hypersensitivity reactions.s profile, publications, research topics, and co-authors
The study is a Phase 1b open label, non-randomized, single institution clinical trial that is designed to evaluate the safety and tolerability of three repeat
Heart transplantation is widely used for the treatment of several heart diseases. Regulatory B cells (Breg cells) serve a critical role in immune tolerance. However, the role of Breg cells in immune tolerance in the context of allogeneic heart transplantation remains poorly understood. The present study aimed to explore the effect of histone deacetylase (HDAC) inhibitor trichostatin A (TSA)‑regulated Breg on the regulation of immune tolerance in heart transplantation. By constructing anallogeneic heart transplantation mouse model, and performing flow cytometry, reverse transcription‑quantitative PCR, western blotting and carboxyfluorescein succinimidyl esterstaining assays, TSA‑regulated Breg cells and their effects on immune tolerance in heart transplantation were evaluated. The results demonstrated that TSA increased the frequency of CD19+CD5+CD1dhigh Breg cells both in vitro and in vivo. Moreover, TSA treatment increased the frequency of IL‑10 and TGF‑β‑producing ...
Reacts with ZAP-70 expressed in T cells, natural killer cells, pro/pre B cells but not in normal mature B cells. The antibody is a useful aid for classification of a subset of chronic lymphocytic leukemias (CLL). In CLL, ZAP-70 expression is closely associated with an unmutated configuration of the immunoglobulin heavy-chain variable region (IgVH) genes (1).|* This product is for in vitro diagnostic use only. The product embodies technology described in US Patent 7,329,502 and pending Canadian Patent Application No. 2,413,475.
B-Cell Chronic Lymphocytic Leukemia - Market Insights, Epidemiology and Market Forecast - 2025 B-Cell Chronic Lymphocytic Leukemia - Market Insights, Epidemiology and Market Forecast - - Market research report and industry analysis - 10975456
Due to its relatively slow clinical progression, B cell chronic lymphocytic leukemia (B-CLL) is classically described as a disease of accumulation rather than proliferation. However, evidence for various forms of clonal evolution suggests that B-CLL clones may be more dynamic than previously assumed. We used a nonradioactive, stable isotopic labeling method to measure B-CLL cell kinetics in vivo. Nineteen patients drank an aliquot of deuterated water (2H2O) daily for 84 days, and 2H incorporation into the deoxyribose moiety of DNA of newly divided B-CLL cells was measured by gas chromatography/mass spectrometry, during and after the labeling period. Birth rates were calculated from the kinetic profiles. Death rates were defined as the difference between calculated birth and growth rates. These analyses demonstrated that the leukemic cells of each patient had definable and often substantial birth rates, varying from 0.1% to greater than 1.0% of the entire clone per day. Those patients with birth ...
CD15 (3-fucosyl-N-acetyl-lactosamine) is a cluster of differentiation antigen - an immunologically significant molecule. CD15 is a carbohydrate adhesion molecule that can be expressed on glycoproteins, glycolipids and proteoglycans. CD15 mediates phagocytosis and chemotaxis, found on neutrophils; expressed in patients with Hodgkin disease, some B-cell chronic lymphocytic leukemias, acute lymphoblastic leukemias, and most acute nonlymphocytic leukemias. It is also called Lewis x and SSEA-1 (stage-specific embryonic antigen 1) and represents a marker for murine pluripotent stem cells, in which it plays an important role in adhesion and migration of the cells in the preimplantation embryo. It is synthezised by FUT4 (fucosyltransferase 4) and FUT9. CD15 is present on almost all Reed-Sternberg cells, including their rare mononuclear variants, and, as such, can be used in immunohistochemistry to identify the presence of such cells in biopsies. The presence of these cells is diagnostic of Hodgkins ...
Toll-like receptors (TLRs) play a key role in B cell-mediated innate and adaptive immunity. It has been shown that interleukin 10 (IL-10)-producing regulatory B cells (B10 cells) can negatively regulate cellular immune responses and inflammation in autoimmune diseases. In this study, we determined the effect of TLR4 signaling on the CD40-activated B10 cell competency. The results demonstrated that LPS and CD40L synergistically stimulated proliferation of mouse splenocytes. The percentage of B10 cells in cultured splenocytes was significantly increased after CD40L stimulation but such increase was diminished by the addition of LPS. Such effects by LPS were only observed in cells from WT but not TLR4−/− mice. IL-10 mRNA expression and protein production in B10 cells from cultured splenocytes were significantly up-regulated by CD40L stimulation but were inhibited after the addition of LPS in a TLR4-dependent manner. This study suggests that LPS-induced TLR4 signaling attenuate CD40L-activated
In order to clarify whether PMNs mediate trogocytosis or phagocytosis of opsonized primary CLL B cells, we performed live-cell time-lapse microscopy experiments. Purified PMNs from healthy donors were cocultured with CLL B-cell samples in the presence of the following anti-CD20 antibodies: wild-type rituximab (RTX-WT), glycoengineered rituximab (RTX-GE), or glycoengineered obinutuzumab (OBZ-GE). Cells were followed for up to 6 hours under the microscope. To our surprise, we could not detect any phagocytic event in up to 6 hours of time-lapse experiments, but only observed the repeated close contact between PMNs and anti-CD20-opsonized CLL B-cell targets, suggesting that trogocytosis rather than phagocytosis takes place. Figure 1A shows selected images of PMNs in contact with CLL B cells opsonized with OBZ-GE. The phase-contrast images obtained at the start of the experiment, before all CLL B cells had settled down to the bottom of the well, show the clear morphological differences between the ...
The balance between immune effector cells and immunosuppressive cells and how this regulates the tumor microenvironment has been well referred to. of Bregs and review our current understanding of Bregs and their inhibition of anti-tumor resistant replies in murine growth versions and tumor sufferers. research, in the past due 1990s, displaying that the adoptive transfer of turned on splenic N cells activated patience and the difference of Testosterone levels cells into suppressor Testosterone levels cells in unsuspecting receiver rodents.33, 34 After these seminal findings, which designated a function for Temsirolimus suppressor B cells in resistant patience, the term regulatory B cells (Bregs) was not coined until nearly 30 years later on, by Bhan and Mizoguchi.35 Mizoguchi et al identified a population of gut-associated, IL-10-creating, CD1d-expressing B cells that suppressed the development of colitis-related intestinal inflammation by downregulating inflammatory cascades.35 However, despite ...
CD5 antigen-like is a protein that in humans is encoded by the CD5L gene. GRCh38: Ensembl release 89: ENSG00000073754 - Ensembl ... 2002). "IgM are associated to Sp alpha (CD5 antigen-like)". Electrophoresis. 23 (7-8): 1203-6. doi:10.1002/1522-2683(200204)23: ... "Entrez Gene: CD5L CD5 molecule-like". Human CD5L genome location and CD5L gene details page in the UCSC Genome Browser. Tissot ...
1994). "CD5 is associated with the human B cell antigen receptor complex". Eur. J. Immunol. 24 (4): 812-6. doi:10.1002/eji. ... It is associated with agammaglobulinemia-6. The B lymphocyte antigen receptor is a multimeric complex that includes the antigen ... PDBe-KB provides an overview of all the structure information available in the PDB for Human B-cell antigen receptor complex- ... Müller B, Cooper L, Terhorst C (1995). "Interplay between the human TCR/CD3 epsilon and the B-cell antigen receptor associated ...
... and CD5. B1a expresses high CD5 level, while B1b expresses low CD5 to almost-absent levels; both are CD19+ and B220low/-.[ ... B1b cells seem to recognize more types of antigens including intracellular antigens. Previously, B1b cell antigen recognition ... making antibodies against antigens and acting as antigen-presenting cells. These B1 cells are commonly found in peripheral ... CD5-CD72 is thought to mediate B cell-B cell interaction. What differentiates B1 cells from other B cells is the variable ...
MCL is a subtype of B-cell lymphoma, due to CD5 positive antigen-naive pregerminal center B-cell within the mantle zone that ... "Low-grade B-cell lymphoma with coexpression of both CD5 and CD10. A report of 3 cases". Arch. Pathol. Lab. Med. 125 (7): 951-3 ...
B-cell-associated antigens such as CD19, CD20, CD22, and CD79a are usually expressed. In contrast to small lymphocytic lymphoma ... and MCL, staining for CD5 is usually negative, and these lymphomas can be further distinguished with CD23 (positive in small ...
Those antibodies are e.g. targeted to CD2, CD3, CD4, CD5, CD8, NK1.1, B220, TER-119, and Gr-1 in mice and CD3 (T lymphocytes), ... Certain antibodies can be used to detect or purify cells with these markers by binding to their surface antigens. A standard ...
... involvement of malignant B-cells that do not express CD5, CD10, or BCL6 and commonly have a translocation between chromosomes ... develops as a consequence of chronic inflammation and specific antigen stimulation. In support of this possibility, there have ... and associations with chronic inflammatory diseases and chronic antigen stimulation; Mantle cell lymphoma differs from DFL by ...
... b-lymphocyte surface antigens CD19, CD20, CD22, CD79a and FMC7, and weak expression of CD5 and CD23. Due to the similarities ... A case has been described as CD20+, CD22+, and CD5-. It can also be CD5+. Another case was described as CD45+, CD19+, CD20+, ... B-lymphocytes have two responsibilities: Production of antibodies - In response to antigens, B-lymphocytes produce and release ... one of its key identifiers is the absence in expression of the surface antigens CD10, CD11c, CD25, CD103 and cyclin D1 - an ...
... antigen, t-cell MeSH D23.050.301.264.035.104 - antigens, cd4 MeSH D23.050.301.264.035.105 - antigens, cd5 MeSH D23.050.301.264. ... antigen, t-cell MeSH D23.050.301.264.894.100 - antigens, cd4 MeSH D23.050.301.264.894.101 - antigens, cd5 MeSH D23.050.301.264. ... antigen, t-cell MeSH D23.101.100.110.104 - antigens, cd4 MeSH D23.101.100.110.105 - antigens, cd5 MeSH D23.101.100.110.107 - ... antigen, t-cell MeSH D23.101.100.894.100 - antigens, cd4 MeSH D23.101.100.894.101 - antigens, cd5 MeSH D23.101.100.894.107 - ...
"CD5 is associated with the human B cell antigen receptor complex". European Journal of Immunology. 24 (4): 812-6. doi:10.1002/ ... Reth M (1992). "Antigen receptors on B lymphocytes". Annual Review of Immunology. 10 (1): 97-121. doi:10.1146/annurev.iy. ... Engels N, Wollscheid B, Wienands J (Jul 2001). "Association of SLP-65/BLNK with the B cell antigen receptor through a non-ITAM ... Brown VK, Ogle EW, Burkhardt AL, Rowley RB, Bolen JB, Justement LB (Jun 1994). "Multiple components of the B cell antigen ...
PKA type I colocalizes with the T-cell and B-cell antigen receptors and causes inhibition of T- and B-cell activation. PKA has ... 1986), "Antibody binding to CD5 (Tp67) and Tp44 T cell surface molecules: effects on cyclic nucleotides, cytoplasmic free ... 1996), "Cyclic AMP-dependent protein kinase (cAK) in human B cells: co-localization of type I cAK (RIα2C2) with the antigen ... In lymphocytes, the intracellular levels of cAMP increase upon antigen-receptor stimulation and even more so in response to ...
CD5+Antigen at the US National Library of Medicine Medical Subject Headings (MeSH) Human CD5 genome location and CD5 gene ... "Entrez Gene: CD5 CD5 molecule". Brown MH, Lacey E (Nov 15, 2010). "A ligand for CD5 is CD5". Journal of Immunology. 185 (10): ... CD5 includes a scavenger receptor cysteine-rich protein domain. T cells express higher levels of CD5 than B cells. CD5 is ... "Evidence for an association between the T cell receptor/CD3 antigen complex and the CD5 antigen in human T lymphocytes". Eur. J ...
... are antigens found on all T cells. They include CD2, CD3, CD5 and CD7. Mario Roederer (October 2004). Cytometry ...
Distinct sets of stereotyped antigen receptors indicate the limited primary structural diversity in antigen-binding sites of ... Production of autoantibodies by CD5-expressing B lymphocytes from patients with chronic lymphocytic leukemia" J Exp Med 169: ... These findings have led to the view that (auto)antigen drive is a promoting factor in the development and evolution of CLL and ... Chiorazzi N, Ferrarini M. (2003) B Cell Chronic Lymphocytic Leukemia: Lessons learned from studies of the B cell antigen ...
FO B cells express high levels of IgD, and CD23; lower levels of CD21 and IgM; and no CD1 or CD5, readily distinguishing this ... Antigen-specific memory B cell development. Annu Rev Immunol. 2005;23:487-513.. ... Two-photon imaging of lymphocyte motility and antigen response in intact lymph node. Science. 2002;296(5574):1869-1873. ...
Tsuge I, Utsumi KR, Ueda R, Takamoto S, Takahashi T (1985). "Assignment of gene coding human T-cell differentiation antigen, ... "The accessory molecules CD5 and CD6 associate on the membrane of lymphoid T cells". J. Biol. Chem. 278 (10): 8564-71. doi: ... Overview of all the structural information available in the PDB for UniProt: P30203 (Human T-cell differentiation antigen CD6) ... "Involvement of CD166 in the activation of human gamma delta T cells by tumor cells sensitized with nonpeptide antigens". J. ...
The ability of T cells to recognize foreign antigens is mediated by the T cell receptor (TCR), which is a surface protein able ... Early, double negative thymocytes express (and can be identified by) CD2, CD5 and CD7. Still during the double negative stage, ... This allows single positive thymocytes to be exposed to a more complex set of self-antigens than is present in the cortex, and ... Cells which do not have a high affinity for self-antigens survive negative selection. At this stage, some cells are also ...
The cells usually do not express CD5, CD10, CD30, or CD138. The neoplastic cells are also usually characterized as being of the ... chimeric antigen receptor T cell therapy using CD19-directed CAR-T cells; and lenalidomide, a drug with multiple anti-tumor ...
It was originally named theta (θ) antigen, then Thy-1 (THYmocyte differentiation antigen 1) due to its prior identification in ... It is one of the "pan T cell markers"(of mice) like CD2, CD5 and CD28. In humans, Thy-1 is also expressed by endothelial cells ... The antigen Thy-1 was the first T cell marker to be identified. Thy-1 was discovered by Reif and Allen in 1964 during a search ... Reif AE, Allen JM (1964). "The AKR thymic antigen and its distribution in leukemias and nervous tissue". J. Exp. Med. 120 (3): ...
MZ B cells shuttle between the blood-filled marginal zone for antigen collection and the follicle for antigen delivery to ... CD5, and CD11b that help to distinguish them phenotypically from follicular (FO) B cells and B1 B cells. MZ B cells are innate- ... MZ B cells respond to a wide spectrum of T-independent, but also T-dependent antigens. It is believed that MZ B cells are ... Moreover, MZ B cells are potent antigen-presenting cells, that are able to activate CD4+ T cells more effectively than FO B ...
The 5-10% of DLBCL, NOS cases in which the neoplastic cells express CD5 have a very poor prognosis that is not improved by even ... Zheng XH, Zhang XY, Dong QQ, Chen F, Yang SB, Li WB (January 2020). "Efficacy and safety of chimeric antigen receptor-T cells ... Gene and protein markers in the neoplastic cells of DLBCL, NOS that have clinical significance include CD5, MYC, BCL2, BCL6, ... Lee YH, Kim CH (July 2019). "Evolution of chimeric antigen receptor (CAR) T cell therapy: current status and future ...
The malignant cells in this disease, unlike FL, stain positive for CD5 and CD23. FL is typically a slowly growing lymphoma with ... lymphocyte function-associated antigen 3, that is involved in activating T-cells), CDKN2A (encoding p16INK4a and p14arf tumor ... infusion of tisagenlecleucel chimeric antigen receptor T cells (i.e. CAR T cells) (i.e. T cells that have been isolated from ... and CD79 but not CD5, CD11c, or CD23 cell surface proteins; genomic analyses reveal that these cells contain t(14:18)(q32:q21.3 ...
CD5, or CD30; and, in particular, by their overexpression of megakaryocyte-associated tyrosine kinase. They are not infected ... and T-cell intracellular antigen-1) but no genetic abnormalities. Indolent T cell lymphoproliferative disorder of the ...
CD5, CD10, surface Ig Frequently occurs outside lymph nodes, very indolent, may be cured by local excision Nodal marginal zone ... Four chimeric antigen receptor CAR-T cell therapies are FDA-approved for non-Hodgkin lymphoma, including lisocabtagene ... By immunohistochemistry, the lymphoma cells expressed CD20, CD5, and Cyclin D1 (high-power view, H&E) Hodgkin lymphoma, nodular ... of lymphomas in adults Lymphocytes of small to intermediate size growing in diffuse pattern CD5 About 50 to 70% Occurs mainly ...
This rearrangement results in the B-cells responding to the ab\normal antigens by taking on features of marginal B-cells and ... The cells almost always express BCL2 and may express MNDA (~70% of cases), CD23 (~33% of cases) and CD5 (~20% of cases) marker ... The lymphocytes have marker protein profiles (e.g. CD20 and Bcl-2 positive; CD5, cyclin D1 and CD10 negative) that are typical ... Campylobacter jejuni-associated disease is more prevalent in individuals who express human leukocyte antigen AI19, B12, or A9 ...
CD64+Antigens at the US National Library of Medicine Medical Subject Headings (MeSH) ...
Mouse Bregs were mainly CD5 and CD1d positive in model of EAE or after exposition of Leishmania major. By contrast mouse Bregs ... "IgG4 production is confined to human IL-10-producing regulatory B cells that suppress antigen-specific immune responses". The ...
Other genes that are commonly thought to be associated with lupus are those in the human leukocyte antigen (HLA) family. There ... ISBN 978-1-4160-2999-1.[page needed] Böhm I (2004). "Increased peripheral blood B-cells expressing the CD5 molecules in ... antigens in pemphigoid List of immunofluorescence findings for autoimmune bullous conditions List of human leukocyte antigen ...
"Direct association of adenosine deaminase with a T cell activation antigen, CD26". Science. 261 (5120): 466-9. doi:10.1126/ ...
In addition to aiding with cytotoxic T cell antigen interactions the CD8 co-receptor also plays a role in T cell signaling. The ... the CD8 co-receptor plays a role in T cell signaling and aiding with cytotoxic T cell antigen interactions. ... This affinity keeps the T cell receptor of the cytotoxic T cell and the target cell bound closely together during antigen- ... Once the T cell receptor binds its specific antigen Lck phosphorylates the cytoplasmic CD3 and ζ-chains of the TCR complex ...
CD1 (a-c, 1A, 1D, 1E) • CD2 • CD3 (γ, δ, ε) • CD4 • CD5 • CD6 • CD7 • CD8 (a) • CD9 • CD10 • CD11 (a, b, c) • CD13 • CD14 • ... 2000). "Characterization of a new member of the TNF family expressed on antigen presenting cells.". Biol. Chem. 380 (12): 1443- ... "BLyS receptor signatures resolve homeostatically independent compartments among naïve and antigen-experienced B cells.". Semin ...
I. Partial characterization of soluble Ki-1 antigen and detection of the antigen in cell culture supernatants and in serum by ... Josimovic-Alasevic O, Dürkop H, Schwarting R, Backé E, Stein H, Diamantstein T (Jan 1989). "Ki-1 (CD30) antigen is released by ... CD30+Antigens at the US National Library of Medicine Medical Subject Headings (MeSH) ... results from cDNA cloning and sequence comparison of the CD30 antigen from different sources". Molecular Immunology. 31 (17): ...
Macrophage-1 antigen (CD11b+CD18). *VLA-4 (CD49d+CD29). *Glycoprotein IIb/IIIa (ITGA2B+ITGB3) ...
Human Antibodies Against Cell Surface Tumor Antigens Selected From Repertoires Displayed on T Cell Chimeric Antigen Receptors" ... CD2, CD3, CD4, CD5, CD7, CD8 - + TdT + + CytogeneticsEdit. Cytogenetic analysis has shown different proportions and frequencies ... TdT is a protein expressed early in the development of pre-T and pre-B cells, whereas CALLA is an antigen found in 80% of ALL ... Chimeric antigen receptors (CARs) have been developed as a promising immunotherapy for ALL. This technology uses a single chain ...
Seligman P. A., Butler C. D., Massey E. J., etal. The p97 antigen is mapped to the q24-qter region of chromosome 3; the same ... Le Beau M. M., Diaz M. O., Plowman G. D., etal. Chromosomal sublocalization of the human p97 melanoma antigen. (англ.) // Hum. ... Plowman G. D., Brown J. P., Enns C. A., etal. Assignment of the gene for human melanoma-associated antigen p97 to chromosome 3 ... Rose T. M., Plowman G. D., Teplow D. B., etal. Primary structure of the human melanoma-associated antigen p97 ( ...
Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) also known as CD66e (Cluster of Differentiation 66e), is a ... 2001). "Heterogeneous RNA-binding protein M4 is a receptor for carcinoembryonic antigen in Kupffer cells". J. Biol. Chem. 276 ( ... CEACAM5, CD66e, CEA, carcinoembryonic antigen related cell adhesion molecule 5. External IDs. HomoloGene: 128801 GeneCards: ... Oikawa S, Nakazato H, Kosaki G (1987). "Primary structure of human carcinoembryonic antigen (CEA) deduced from cDNA sequence". ...
van Rhenen A., van Dongen G. A., Kelder A., et al. The novel AML stem cell associated antigen CLL-1 aids in discrimination ...
"Interaction of glycogen synthase kinase 3beta with the DF3/MUC1 carcinoma-associated antigen and beta-catenin". Molecular and ...
Ebert LM, McColl SR (2002). "Up-regulation of CCR5 and CCR6 on distinct subpopulations of antigen-activated CD4+ T lymphocytes ... This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may ... dendritic cells induce antitumor immunity when genetically fused with nonimmunogenic tumor antigens". J. Immunol. 167 (11): ...
W ten sposób zidentyfikowano limfocyty Ts jako populację o niskiej ekspresji białek CD5[16], CD45RB[17] i CD45RC[18]. Problemem ... Antigen-specific peripheral shaping of the natural regulatory T cell population. „J Exp Med". 205 (13), s. 3105-3117, grudzień ... De novo production of antigen-specific suppressor cells in vivo. „Nat Protoc". 1 (2), s. 653-661, 2006. PMID: 17802642. ... Regulation of the immune response to tumor antigens. X. Activation of third-order suppressor T cells that abrogate anti-tumor ...
It is also called Lewis x and SSEA-1 (stage-specific embryonic antigen 1) and represents a marker for murine pluripotent stem ... CD15 Antigen at the US National Library of Medicine Medical Subject Headings (MeSH) ... CD15 (3-fucosyl-N-acetyl-lactosamine) is a cluster of differentiation antigen - an immunologically significant molecule. CD15 ...
A new ligand for human leukocyte antigen class II antigens". The Journal of Experimental Medicine. 176 (2): 327-37. doi:10.1084 ... A new ligand for human leukocyte antigen class II antigens". The Journal of Experimental Medicine. 176 (2): 327-37. doi:10.1084 ... antigen processing and presentation of exogenous peptide antigen via MHC class II. ... antigen binding. • transmembrane signaling receptor activity. • MHC class II protein binding. Cellular component. • membrane. • ...
CD1 (a-c, 1A, 1D, 1E) • CD2 • CD3 (γ, δ, ε) • CD4 • CD5 • CD6 • CD7 • CD8 (a) • CD9 • CD10 • CD11 (a, b, c) • CD13 • CD14 • ... CD97 antigen je protein koji je kod ljudi kodiran CD97 genom.[1][2][3] ... 2001). "Tissue distribution of the human CD97 EGF-TM7 receptor". Tissue Antigens 57 (4): 325-31. PMID 11380941. doi:10.1034/j. ... "Expression cloning and chromosomal mapping of the leukocyte activation antigen CD97, a new seven-span transmembrane molecule of ...
"Entrez Gene: ITGB3 integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)".. *^ May, K. E.; Villar, J.; Kirtley, S.; ... CD61+Antigens at the US National Library of Medicine Medical Subject Headings (MeSH) ...
B cells can present antigens to a specialized group of helper T cells called TFH cells. If an activated TFH cell recognizes the ... Roles of T cell-B-cell-activating molecule (5c8 antigen) and CD40 in contact-dependent help". Journal of Immunology. 149 (12): ... It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In ... Grewal, IS; Xu, J; Flavell, RA (7 December 1995). "Impairment of antigen-specific T-cell priming in mice lacking CD40 ligand". ...
antigen processing and presentation of peptide antigen via MHC class I. • antigen processing and presentation of exogenous ... antigen processing and presentation of exogenous peptide antigen via MHC class I. • lipoprotein transport. • negative ... peptide antigen via MHC class I, TAP-dependent. • platelet degranulation. • MyD88-dependent toll-like receptor signaling ...
Primarily, the VCAM-1 protein is an endothelial ligand for VLA-4 (Very Late Antigen-4 or integrin α4β1) of the β1 subfamily of ...
... uveitis antigens induce CXCR3- and CXCR5-expressing lymphocytes and immature dendritic cells to migrate (англ.) // Blood (англ ...
There are case reports of experimental treatments with eculizumab, a monoclonal antibody against CD5 that blocks part of the ... "Thomsen-Friedenreich antigen exposure as a cause of Streptococcus pyogenes-associated hemolytic-uremic syndrome". Clinical ...
antigen binding. • virus receptor activity. • protein binding. • transmembrane signaling receptor activity. • identical protein ...
CD1 (a-c, 1A, 1D, 1E) • CD2 • CD3 (γ, δ, ε) • CD4 • CD5 • CD6 • CD7 • CD8 (a) • CD9 • CD10 • CD11 (a, b, c) • CD13 • CD14 • ... 1996). "CD88 antibodies specifically bind to C5aR on dermal CD117+ and CD14+ cells and react with a desmosomal antigen in human ...
CD74 (англ. HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain) - мембранный белок ... II histocompatibility antigen gamma chaingamma chain of class II antigensIiHLA-DR antigens-associated invariant chainIa antigen ... Riberdy J.M., Newcomb J.R., Surman M.J., Barbosa J.A., Cresswell P. HLA-DR molecules from an antigen-processing mutant cell ... Machamer C.E., Cresswell P. Biosynthesis and glycosylation of the invariant chain associated with HLA-DR antigens (англ.) // ...
1997). "The Oka blood group antigen is a marker for the M6 leukocyte activation antigen, the human homolog of OX-47 antigen, ... 1992). "Human leukocyte activation antigen M6, a member of the Ig superfamily, is the species homologue of rat OX-47, mouse ... Kasinrerk W, Fiebiger E, Stefanová I, Baumruker T, Knapp W, Stockinger H (1992). "Human leukocyte activation antigen M6, a ... Ok blood group system at BGMUT Blood Group Antigen Gene Mutation Database at NCBI, NIH ...
CD1 (a-c, 1A, 1D, 1E) • CD2 • CD3 (γ, δ, ε) • CD4 • CD5 • CD6 • CD7 • CD8 (a) • CD9 • CD10 • CD11 (a, b, c) • CD13 • CD14 • ... 1991). „Expression of the YB5.B8 antigen (c-kit proto-oncogene product) in normal human bone marrow". Blood. 78 (1): 30-7. PMID ... 2003). „Signal transduction-associated and cell activation-linked antigens expressed in human mast cells". Int. J. Hematol. 75 ...
Tissue Antigens (англ.)русск. : journal. - 2007. - Vol. 68, no. 6. - P. 509-517. - DOI:10.1111/j.1399-0039.2006.00726.x. - PMID ...
... is not routinely used to treat Hodgkin's lymphoma due to the lack of CD20 surface antigens in most cases. The use of rituximab ... CD5+. *naive B cell (CLL/SLL). *mantle zone (Mantle cell). CD22+. *Prolymphocytic ...
T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell. • T cell antigen ... CD1 (a-c, 1A, 1D, 1E) · CD2 · CD3 (γ, δ, ε) · CD4 · CD5 · CD6 · CD7 · CD8 (a) · CD9 · CD10 · CD11 (a, b, c, d) · CD13 · CD14 · ...
CD5 antigen-likeAdd BLAST. 331. Amino acid modifications. Feature key. Position(s). DescriptionActions. Graphical view. Length ... CD5 antigen-like. Alternative name(s):. Apoptosis inhibitor expressed by macrophages1 Publication. Manual assertion based on ... sp,Q9QWK4,CD5L_MOUSE CD5 antigen-like OS=Mus musculus OX=10090 GN=Cd5l PE=1 SV=3 ...
CD5-fl, CD5-F378, CD5-F429, CD5-F441, CD5-F463, CD5-428*, and CD5-462*) were stained with the isotype-matched control rat anti- ... CD5-F378, CD5-F429, CD5-F441, and CD5-F463), and truncated forms (CD5-428* and CD5-462*; numbered according to the last amino ... or B-cell antigen receptor signaling by CD5 was proposed based on studies of thymocytes and peritoneal B-1a cells from CD5- ... CD5 negatively regulates the T-cell antigen receptor signal transduction pathway: involvement of SH2-containing phosphotyrosine ...
CD5 Antigen-Like - Proteins. Product filter CD5 Antigen-Like molecule 4-1BB Ligand 4-1BB Receptor 6-Phosphogluconate ... CD5 Antigen-Like CDNF Cell Death-Inducing DFFA-Like Effector C Claudin-4 Club Cell Protein Clusterin CNTF Collagen Triple Helix ... Prostate Specific Antigen Prostate-Specific Gene-1 Protein Delta Homolog 1 Protein disulfide-isomerase A3 Prouroguanylin PTHrP ...
The ectodomains of the lymphocyte scavenger receptors CD5 and CD6 interact with tegumental antigens from ,i,Echinococcus ... antigen P-29) for CD6, as their potential interactors. Further in vitro assays demonstrate that membrane-bound or soluble CD5 ... CD5 and CD6 are two highly homologous class I SRs mainly expressed on all T cells and the B1a cell subset, and involved in the ... We report herein the interaction of CD5 and CD6 lymphocyte surface receptors with Echinococcus granulosus sensu lato (s.l.). ...
Anti- CD5 (Lymphocyte antigen T1/Leu 1, p56 62, T cell surface glycoprotein CD5, T1) (MaxLight 750). Catalog Number. Pack Size ...
Pan-T antigens are antigens found on all T cells. They include CD2, CD3, CD5 and CD7. Mario Roederer (October 2004). Cytometry ...
Secreted protein that acts as a key regulator of lipid synthesis: mainly expressed by macrophages in lymphoid and inflammed tissues and regulates mechanisms in inflammatory responses, such as infection or atherosclerosis. Able to inhibit lipid droplet size in adipocytes. Following incorporation into mature adipocytes via CD36-mediated endocytosis, associates with cytosolic FASN, inhibiting fatty acid synthase activity and leading to lipolysis, the degradation of triacylglycerols into glycerol and free fatty acids (FFA). CD5L-induced lipolysis occurs with progression of obesity: participates in obesity-associated inflammation following recruitment of inflammatory macrophages into adipose tissues, a cause of insulin resistance and obesity-related metabolic disease. Regulation of intracellular lipids mediated by CD5L has a direct effect on transcription regulation mediated by nuclear receptors ROR-gamma (RORC). Acts as a key regulator of metabolic switch in T-helper Th17 cells. Regulates the ...
CD5 antigen-like is a protein that in humans is encoded by the CD5L gene. GRCh38: Ensembl release 89: ENSG00000073754 - Ensembl ... 2002). "IgM are associated to Sp alpha (CD5 antigen-like)". Electrophoresis. 23 (7-8): 1203-6. doi:10.1002/1522-2683(200204)23: ... "Entrez Gene: CD5L CD5 molecule-like". Human CD5L genome location and CD5L gene details page in the UCSC Genome Browser. Tissot ...
CD5 Molecule, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene ... Suggested Antigen Peptide Sequences for CD5 Gene. GenScript: Design optimal peptide antigens:. *Lymphocyte antigen T1/Leu-1 ( ... Animal Models for CD5 Gene. MGI Knock Outs for CD5:. * Cd5 Cd5,tm1.1(KOMP)Mbp, ... GeneCards Summary for CD5 Gene CD5 (CD5 Molecule) is a Protein Coding gene. Diseases associated with CD5 include Thymus Cancer ...
CD5 antigen-like. Names. CD5 antigen-like (scavenger receptor cysteine rich family). apoptosis inhibitor 6. apoptosis inhibitor ... NM_005894.2 → NP_005885.1 CD5 antigen-like isoform 1 precursor. See identical proteins and their annotated locations for NP_ ... CD5L CD5 molecule like [Homo sapiens] CD5L CD5 molecule like [Homo sapiens]. Gene ID:922 ... CD5 molecule likeprovided by HGNC. Primary source. HGNC:HGNC:1690 See related. Ensembl:ENSG00000073754 MIM:602592; Vega: ...
One novel form of anti-T-cell therapy is the immunoconjugate CD5-Plus … ... This agent is composed of the murine IgG1 monoclonal antibody H65, which is directed toward the CD5+ antigen; and ricin A chain ... Effects of an anti-CD5 immunoconjugate (CD5-plus) in recent onset type I diabetes mellitus: a preliminary investigation. The ... We conducted a dose-escalation study using CD5-Plus given as an intravenous infusion for 5 consecutive days. Fifteen subjects ( ...
Mouse Monoclonal CD5 antibody [L17F12] (PerCP). Validated in FACS. Tested in Human. ... Antigen Species Human Immunogen Human acute lymphoblastic leukemia (ALL) T cells Purification Purified by size-exclusion ... There are currently no reviews for CD5 antibody [L17F12] (PerCP) (GTX00505-16). Be the first to share your experience with this ... There are currently no references for CD5 antibody [L17F12] (PerCP) (GTX00505-16). Be the first to share your publications with ...
Shop a large selection of products and learn more about CD5 Rat anti-Mouse, FITC, Clone: 53-7.3, eBioscience 500 µg; FITC 500 ... CD5 antigen p56-62, T-cell surface glycoprotein CD5, lymphocyte antigen T1/Leu-1. ... CD5 is present on all mature T-lymphocytes, on most of thymocytes and on many T-cell leukemias and lymphomas. CD5 also reacts ... CD5 is expressed by many T cell leukemia, lymphomas, and activated T cells. Diseases associated with CD5 dysfunction include ...
CD5/54/F6, Novus CD5 Antibody; Alexa Fluor 750; 0.1 mL. ... Shop a large selection of products and learn more about CD5 ... CD5 antigen, CD5 antigen (p56-62), CD5 molecule, LEU1T-cell surface glycoprotein CD5, Lymphocyte antigen T1/Leu-1, T1. ... CD5. Immunogen. Human CD5 recombinant protein (C5/473); A synthetic peptide from the intracellular region of human CD5 (CD5/54/ ... Recognizes a 67kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95% of thymocytes and 72% of ...
CD5(+) B cells are receptive to cytokines and interleukin-10 seems to be influential in the regulation of some of these CD5(+) ... The CD5(+) B cell population is prominent in early life and produce low avidity and, thereby, polyreactive antibodies. ... CD5(+) B cells probably play a role in setting up the idiotype network, antigen presentation and tolerance induction. B cells ... Indeed, activated CD5(+) B cells do proliferate, following CD5 engagement, while resting CD5(+) B cells do not. Moreover, three ...
... and CD5(-) subsets, individual CD19(+)/ IgM+/CD5(+) or CD5(-) B cells were sorted and non-productive as well as productive VH ... To analyze the immunoglobulin repertoire of human IgM+ B cells and the CD5(+) ... as well as selection before and after antigen exposure. Moreover, the influences on the repertoires of CD5(+) and CD5(-) B ... To analyze the immunoglobulin repertoire of human IgM+ B cells and the CD5(+) and CD5(-) subsets, individual CD19(+)/ IgM+/CD5 ...
Preclinical targeting of aggressive T-cell malignancies using anti-CD5 chimeric antigen receptor. / Chen, K. H.; Wada, M.; Pinz ... Preclinical targeting of aggressive T-cell malignancies using anti-CD5 chimeric antigen receptor. Leukemia. 2017 Oct 1;31(10): ... title = "Preclinical targeting of aggressive T-cell malignancies using anti-CD5 chimeric antigen receptor", ... T1 - Preclinical targeting of aggressive T-cell malignancies using anti-CD5 chimeric antigen receptor ...
Mouse monoclonal CD5 antibody [OX-19] conjugated to FITC. Tested in Rat. Cited in 2 publication(s). Immunogen corresponding to ... CD5 antigen (p56 62) antibody. *CD5 antigen antibody. *CD5 molecule antibody. *CD5_HUMAN antibody ... https://www.abcam.com/Multicolor-Human-B-cell-marker-panel-CD5-PE-CD19-APC-CD20-FITC-and-CD45-PE-Cy7-ab106071.html. We have ...
Autologous T-Cells Expressing a Second Generation CAR for Treatment of T-Cell Malignancies Expressing CD5 Antigen (MAGENTA). ... CD5 antibodies have been used to treat people with T-cell leukemia and lymphoma. For this study, anti-CD5 has been changed so ... CD5.CAR/28zeta CAR T cells Trial Summary & Details Ages: up to 75 Years. Condition: T-cell Acute Lymphoblastic Lymphoma, T-non- ... These CD5 chimeric receptor T cells with CD28 are investigational products not approved by the Food and Drug Administration . ...
CD30 +; CD38 +; CD45 +; CD 54 +; CD71 +; HLA-DR +; EMA + (epithelial membrane antigen); CD2 -; CD3 -; CD4 -; CD5 -, CD8 -; CD19 ... The cells do not express B-cell lineage restricted antigens or kappa or lambda immunoglobulin light chains or T-cell lineage- ...
Antigen Expression CD1a -; CD2 -; CD3 -; CD4 -; CD5 -; CD8 -; CD10 +; CD13 +; CD38 +; CD71 +; HLA DR + ... They are are negative for CB1, Leu 1 (CD5), Leu2 (CD8), Leu3 (CD4), Leu4 (CD3), Leu5 (CD2), Leu6 (CD1a), Leu9, Leu M1 (CD15), ... The cells are positive for the beta-2-microglobulin, Leu12, My7 (CD13), OKT9 (CD71), OKT10 (CD38) and CALLA (CD10) antigens. ...
CD5 PE - read details of Sysmex Europe GmbH antibodies in the SelectScience.net Antibody products and suppliers directory ... CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular ... CD5 (T1) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T- ... CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and ...
CD43 and NK-cell antigen CD56; negative for CD3, CD5, CD57 and CD11. Large groups of Epstein-Barr virus-encoded RNA-positive ... Most of the background cells are positive for (H) CD3, (I) CD2, (J) CD5, (K) CD7 and (L) CD8. The large atypical cells are ... Most of the background cells are positive for (H) CD3, (I) CD2, (J) CD5, (K) CD7 and (L) CD8. The large atypical cells are ... CD45RO, MT1, CD3, OPD4, βF1, CD4, CD8 and CD5. Markers of Reed-Sternberg cells. CD15, CD30, peanut agglutinin, fascin and LMP1 ...
Cd5, CD5 antigen. Gene Synonym(s). Strain of Origin. multiple strains. Chromosome. 19. ...
B1a = CD19+CD5+CD23−; B1b = CD19+CD5−CD23−; B2 = CD19+CD5−CD23+ (see gating in Supplemental Fig. 2H). (B and C) Representative ... E-G) Representative gating (E) and the quantification of (F) NP+ Igλ1+ and (G) NP+ Igλ2+ B1a cells (CD5+CD23−) isolated from ... Optimal Development of Mature B Cells Requires Recognition of Endogenous Antigens. Mark Noviski, Corey Tan, John Huizar, ... Optimal Development of Mature B Cells Requires Recognition of Endogenous Antigens. Mark Noviski, Corey Tan, John Huizar, ...
Suggested Antigen Peptide Sequences for UBP1 Gene. GenScript: Design optimal peptide antigens:. *Transcription factor LBP-1 ( ... GeneTex Cd5 antibody for UBP1 * Search for UBP1 Antibodies at ProSci * Custom Antibody Services ...
CD5 antigen: Cd5 (mice) and CD5 (humans). A list of human gene names is available at http://www.genenames.org/guidelines.html ...
CD5 Monoclonal Antibody, APC conjugate from Invitrogen for Flow Cytometry applications. This antibody reacts with Human samples ... Protein Aliases: CD5; CD5 antigen (p56-62); CD5 antigen p56-62; Lymphocyte antigen T1/Leu-1; T-cell surface glycoprotein CD5 ... Cite CD5 Monoclonal Antibody (CRIS1), APC. The following antibody was used in this experiment: CD5 Monoclonal Antibody (CRIS1 ... The CD20 antigen is present on human pre-B lymphocytes and on B-lymphocytes atall stage of maturation, except on plasma cells. ...
The expression of CD5 by a small B cell subset characterizes a developmentally and functionally distinct lineage of B cells ... CD5 plays a role in the interaction of T and B cells and is a counter-receptor for CD72. - Belgique ... CD5, which is recognized by the monoclonal antibody 53-7.3, is a 67 kDa protein also known as Ly-1. It is expressed by a ... Distribution of antigen: B cells, lymphocytes, T cells, thymocytes * Product format: Reagents are supplied in buffer containing ...
  • Anti- CD5 (Lymphocyte antigen T1/Leu 1, p56 62, T cell surface glycoprotein CD5, T1) (MaxLight 750) by USBiological, Cat. (lucerna-chem.ch)
  • CD5 is a 67 kDa human T-lymphocyte single-chain transmembrane glycoprotein. (fishersci.com)
  • In humans, CD5 is a 67kDa T lymphocyte single chain transmembrane glycoprotein. (thermofisher.com)
  • Clone UCHT2 reacts with human CD5, a 67 kDa single-chain transmembrane glycoprotein. (miltenyibiotec.com)
  • This antibody reacts with the cell surface glycoprotein CD5, a 67KDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes). (abnova.com)
  • The CD5 antigen is a single-chain transmembrane glycoprotein with a molecular weight of 67 kDa. (beckman.com)
  • McAlister MS, Davis B, Pfuhl M, Driscoll PC: NMR analysis of the N-terminal SRCR domain of human CD5: engineering of a glycoprotein for superior characteristics in NMR experiments. (exbio.cz)
  • It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains.The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. (nordicbiosite.com)
  • The CD5(+) B cell population is prominent in early life and produce low avidity and, thereby, polyreactive antibodies. (biomedsearch.com)
  • CD5 antibodies have been used to treat people with T-cell leukemia and lymphoma. (emilywhiteheadfoundation.org)
  • CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies.Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc. (selectscience.net)
  • Spleen cells of BALB/c mice were stained with CD5 antibodies and analyzed by flow cytometry using the MACSQuant® Analyzer. (miltenyibiotec.com)
  • Ledbetter, J. A. and Herzenberg, L. A. (1979) Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. (miltenyibiotec.com)
  • CLL B-lymphocytes typically show B-cell surface antigens, as demonstrated by CD19, CD20 dim , CD21, and CD23 monoclonal antibodies. (medscape.com)
  • Conversely, STAT3, CD5, and BLNK were in the immunoprecipitate of CLL cells immunoprecipitated with CK2 antibodies. (aacrjournals.org)
  • Furthermore, siRNA knockdown of CD5 or BLNK, or treatment with CD5-neutralizing antibodies significantly reduced the levels of serine pSTAT3 in CLL cells. (aacrjournals.org)
  • The potential of this technology is illustrated by its use in revealing a broad-spectrum of pre-existing anti-lipid antibodies in blood circulation and monitoring the epitope spreading of autoantibody reactivities among protein, carbohydrate, and lipid antigens in experimental autoimmune encephalomyelitis (EAE). (mdpi.com)
  • CD5 (CD5 Molecule) is a Protein Coding gene. (genecards.org)
  • The question of whether CD5 is a marker of activation or a molecule specific for a B cell lineage remains unresolved because evidence in support or against a separate lineage are still a matter for debate. (biomedsearch.com)
  • The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. (selectscience.net)
  • Description: The 53-7.3 monoclonal antibody reacts with mouse CD5, a 67 kDa protein expressed by a majority of thymocytes, mature T cells and a subset of B cells. (fishersci.com)
  • CD5 Monoclonal antibody specifically detects CD5 in Human samples. (fishersci.com)
  • The following antibody was used in this experiment: CD5 Monoclonal Antibody (CRIS1), APC from Thermo Fisher Scientific, catalog # MA1-19474, RRID AB_1072518. (thermofisher.com)
  • CD5, which is recognized by the monoclonal antibody 53-7.3, is a 67 kDa protein also known as Ly-1. (miltenyibiotec.com)
  • Mouse monoclonal antibody raised against human CD5. (abnova.com)
  • Flow cytometric analysis of human peripheral blood lymphocytes with CD5 monoclonal antibody, clone L17F12 (Cat # MAB13739). (abnova.com)
  • Mouse monoclonal antibody raised against native CD5. (abnova.com)
  • BL1a monoclonal antibody (ref. 6T-CD5.5) was used as a CD5 reference monoclonal antibody during HLDA/6. (beckman.com)
  • Engleman EG, Warnke R, Fox RI, Dilley J, Benike CJ, Levy R: Studies of a human T lymphocyte antigen recognized by a monoclonal antibody. (exbio.cz)
  • To analyze the immunoglobulin repertoire of human IgM+ B cells and the CD5(+) and CD5(-) subsets, individual CD19(+)/ IgM+/CD5(+) or CD5(-) B cells were sorted and non-productive as well as productive VH gene rearrangements were amplified from genomic DNA and sequenced. (nih.gov)
  • Chronic lymphocytic leukemia (CLL) is characterized by a clonal expansion of CD5 + CD19 + B cells found predominantly in the peripheral blood and lymphoid tissues. (bloodjournal.org)
  • Freedman et al [35] studied the immunophenotype of 100 B-CLL patients and found that all cases expressed Ia, CD19, and CD20 (pan B-cell antigens). (cancernetwork.com)
  • Fully human CD19-specific chimeric antigen receptors for T-cell therapy. (amedeo.com)
  • 2 The tumour cells express monotypic surface Ig (sIg) and sometimes cytoplasmic Ig (cIg) (usually IgM), moreover the pan B cell antigens CD19, CD20, CD22, and CD79a. (bmj.com)
  • Cherukuri A, Cheng PC, Pierce SK (2001a) The role of the CD19/CD21 complex in B-cell processing and presentation of complement-tagged antigens. (springer.com)
  • Cherukuri A, Cheng P, Sohn H, Pierce S (2001b) The CD19/CD21 complex functions to prolong B-cell antigen receptor signaling from lipid rafts. (springer.com)
  • It is characterized by the clonal accumulation of CD5 + B-cells expressing CD19, CD23 and low surface IgM. (haematologica.org)
  • The long road to the first FDA-approved gene therapy: chimeric antigen receptor T cells targeting CD19. (upenn.edu)
  • The neoplastic cells infiltrate the reactive follicles, showing follicular colonization, and have typical lymphoepithelial lesion [ 3 ].The neoplastic cells express pan B-cell antigens (CD19, CD20, CD22). (ispub.com)
  • In IVLBCL, the tumor cells express B-cell associated antigens (CD20, CD19) and may co-express CD5 and CD10. (renalandurologynews.com)
  • FACS analysis of human peripheral blood using GTX00505-16 CD5 antibody [L17F12] (PerCP). (genetex.com)
  • There are currently no references for CD5 antibody [L17F12] (PerCP) (GTX00505-16) . (genetex.com)
  • The antibody used in this study is called anti-CD5. (emilywhiteheadfoundation.org)
  • This antibody sticks to T-cell leukemia or lymphoma cells because of a substance on the outside of these cells called CD5. (emilywhiteheadfoundation.org)
  • Importantly, this technology allows simultaneous detection of serum antibody activities among the three major classes of antigens, i.e. , lipids, carbohydrates, and proteins. (mdpi.com)
  • The proliferation index evaluated with antibody against Ki67 antigen (MIB-1) reached a value of 70% (Fig. 9 ). (upmc.edu)
  • CD5 antibody is a T-cell associated marker that is also expressed by two B-cell neoplasms: lymphocytic leukemia and mantle cell lymphoma. (biocare.net)
  • The CD20 antigen is present on human pre-B lymphocytes and on B-lymphocytes atall stage of maturation, except on plasma cells. (thermofisher.com)
  • Low level expression of the CD20 antigen has been detected on normal T lymphocytes. (thermofisher.com)
  • It was recognized in line with the illustration showing B-cell antigens CD20 and also CD5. (dailystrength.org)
  • General expression of CD20 revealed the B cell nature of the tumor infiltrate, but it was interesting that CD5 expression in about 50% of tumor cells was positive. (ispub.com)
  • Immunohistochemical study showed positivity for leukocytic common antigen (CD45), B-cell marker (CD20) and bcl 6. (curehunter.com)
  • A large fraction of newly rearranged BCRs bind to self-antigens, including nucleic acids, but these specificities are efficiently removed from the repertoire by receptor editing and deletion and tolerance mechanisms such as anergy control residual self-reactivity in the periphery ( 2 - 4 ). (jimmunol.org)
  • CD5 antigen-like is a protein that in humans is encoded by the CD5L gene. (wikipedia.org)
  • In addition, stimulation of Jurkat cells or normal phytohemagglutinin-expanded T lymphoblasts through TCR-CD3 induced rapid tyrosine phosphorylation of several protein substrates, which was substantially diminished after CD5 cross-linking. (nih.gov)
  • Recognizes a 67kDa transmembrane protein, which is identified as CD5. (fishersci.com)
  • ab24500 recognises CD5 protein. (abcam.com)
  • Purified recombinant protein of full-length mouse CD5 antigen (Cd5), with C-terminal MYC/DDK tag, was expressed in HEK293T cells. (creativebiomart.net)
  • CD5 is a transmembrane protein that is expressed on the surface of T cells and a subset of B cells. (sciencemag.org)
  • Retinal pigment epithelium and microglia express the CD5 antigen-like protein, a novel autoantigen in age-related macular degeneration. (duke.edu)
  • Effects of an anti-CD5 immunoconjugate (CD5-plus) in recent onset type I diabetes mellitus: a preliminary investigation. (nih.gov)
  • Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large B-cell lymphoma. (fishersci.com)
  • Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. (fishersci.com)
  • Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. (fishersci.com)
  • Anti-CD5 does not react with granulocytes or monocytes. (fishersci.com)
  • Here, we report a robust anti-CD5 CAR (CD5CAR) transduced into a human NK cell line NK-92 that can undergo stable expansion ex vivo. (elsevier.com)
  • For this study, anti-CD5 has been changed so that instead of floating free in the blood it is now joined to the T cells. (emilywhiteheadfoundation.org)
  • Flow cytometry analysis (surface staining) of CD5 in human peripheral blood cells with anti-CD5 (L17F12) APC. (exbio.cz)
  • Together, these thymic and peripheral mechanisms ensure that T cells are negatively selected against all self-antigens. (rupress.org)
  • Autoimmune hemolytic anemia (AIHA) consists of loss of tolerance to self-antigens on red blood cells (RBCs) in the humoral compartment. (haematologica.org)
  • Another surprise is that such diverse receptors in an individual do not react with any structures contained in the self body (self-antigens) in principle, which is called self-tolerance. (springer.com)
  • The cells are positive for the beta-2-microglobulin, Leu12, My7 (CD13), OKT9 (CD71), OKT10 (CD38) and CALLA (CD10) antigens. (atcc.org)
  • CD5 is present on all mature T-lymphocytes, on most of thymocytes and on many T-cell leukemias and lymphomas. (fishersci.com)
  • The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. (fishersci.com)
  • CD5 is found on all mature T lymphocytes and on most thymocytes. (beckman.com)
  • CD5 is a ligand for the CD72 antigen, which is present on B lymphocytes. (beckman.com)
  • Chronic lymphocytic leukemia (CLL), the most common leukemia in the Western Hemisphere, is characterized by a gradual accumulation of mature-appearing lymphocytes coexpressing typical B-cell surface antigens ( 1 ) and CD5. (aacrjournals.org)
  • In contrast, antigen receptors on B and T lymphocytes diversify enormously in each individual: genes for these receptors composed of multiple various parts are assembled randomly through gene recombination during development of these cells throughout the life of the individuals. (springer.com)
  • 0.75 mm) of superficial spreading melanoma the vast majority of tumor-infiltrating lymphocytes (TIL) belong to the CD4+ subset and frequently express CD45RA antigens. (curehunter.com)
  • Although IHC is not commonly necessary in differentiation, epithelial membrane antigen (EMA) highlights most mature sebocytes in sebaceoma, whereas its expression is uncommon in BCC. (thefreelibrary.com)
  • The distinct V H repertoire that is found in B-1 cells has led to the hypothesis that the specificity of the B cell antigen receptor (BCR) 1 may in fact determine the differentiation of B cells into this subset ( 5 )( 6 ). (rupress.org)
  • CD5 and CD6 are two highly homologous class I SRs mainly expressed on all T cells and the B1a cell subset, and involved in the fine tuning of activation and differentiation signals delivered by the antigen-specific receptors (TCR and BCR, respectively), to which they physically associate. (classicistranieri.com)
  • We report herein the interaction of CD5 and CD6 lymphocyte surface receptors with Echinococcus granulosus sensu lato (s . l . (classicistranieri.com)
  • To determine the developmental potential of B cells bearing two distinct B cell antigen receptors (BCRs), one favoring B-1 and the other favoring B-2 cell development, we crossed V H 12 insertion mice with mice bearing either V H B1-8 or V H glD42. (rupress.org)
  • The expression of CD5 by a small subset of B cells characterizes a developmentally and functionally distinct lineage of B cells called B-1 cells. (fishersci.com)
  • CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. (fishersci.com)
  • Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells. (selectscience.net)
  • Our analysis of bone marrow samples from 50 patients with MDS showed aberrant expression of differentiation antigens in the myelomonocytic lineage. (bloodjournal.org)
  • Expression of one or more pan-T-cell antigens (CD45RO, CD2, CD3, CD5, and CD7), absence of pan-B-cell antigens, and histopathologic findings combine to make the diagnosis ( 8 ). (aacrjournals.org)
  • Freedman AS, Freeman G, Whitman J, Segil J, Daley J, Levine H, Nadler LM: Expression and regulation of CD5 on in vitro activated human B cells. (exbio.cz)
  • they express high levels of B220, IgD, and CD23 and moderate levels of IgM, and lack surface CD5 expression (for review see references 1, 2). (rupress.org)
  • Design and Methods In order to study the pathogenesis of autoimmune hemolytic anemia, we developed a murine model with RBC-specific expression of a model antigen carrying epitopes from hen egg lysozyme and ovalbumin. (haematologica.org)
  • Expression of MUC-1 and ALK was determined immunohistochemically after heat-induced antigen retrieval. (aacrjournals.org)
  • It has thought that CD5-expression is a marker for unusual and aggressive clinical course. (ispub.com)
  • Fig . 2B ) With exception of CD5 expression, the morphological findings and growth pattern of the neoplastic cells, led us to diagnosis of a malignant small cell B-cell non-Hodgkin lymphoma and sub-typed as extranodal marginal zone B-cell lymphoma of MALT type. (ispub.com)
  • Chen found that expression of CD5+CD19+ IL-10 Bregs in the peripheral blood of patients with hepatocellular carcinoma was higher than that in healthy controls, suggesting that Bregs play a crucial role in the development and prognosis of carcinogenesis [9]. (termedia.pl)
  • The alterations of CD45RA and CD45RO antigens expression on T helper cells in prediabetics suggest the significant role of naive or/and memory CD4+ T cell subsets in the pathogenesis of diabetes type 1. (curehunter.com)
  • The negative regulation of T- or B-cell antigen receptor signaling by CD5 was proposed based on studies of thymocytes and peritoneal B-1a cells from CD5-deficient mice. (nih.gov)
  • Here, we show that CD5 is constitutively associated with phosphotyrosine phosphatase activity in Jurkat T cells. (nih.gov)
  • CD5 was found associated with the Src homology 2 (SH2) domain containing hematopoietic phosphotyrosine phosphatase SHP-1 in both Jurkat cells and normal phytohemagglutinin-expanded T lymphoblasts. (nih.gov)
  • CD5 co-cross-linking with the TCR-CD3 complex down-regulated the TCR-CD3-increased Ca2+ mobilization in Jurkat cells. (nih.gov)
  • Further in vitro assays demonstrate that membrane-bound or soluble CD5 and CD6 forms differentially modulate the pro- and anti-inflammatory cytokine release induced following peritoneal cells exposure to E . granulosus s . l . tegumental components. (classicistranieri.com)
  • Pan-T antigens are antigens found on all T cells. (wikipedia.org)
  • CD5 also reacts with a subpopulation of activated B-cells and may act as a receptor in regulating T-cell proliferation. (fishersci.com)
  • CD5 is expressed by many T cell leukemia, lymphomas, and activated T cells. (fishersci.com)
  • The role of CD5-expressing B cells in health and disease (review). (biomedsearch.com)
  • CD5(+) B cells are receptive to cytokines and interleukin-10 seems to be influential in the regulation of some of these CD5(+) B cells. (biomedsearch.com)
  • However, we suggest the possibility of different kind of CD5(+) B cells. (biomedsearch.com)
  • Indeed, activated CD5(+) B cells do proliferate, following CD5 engagement, while resting CD5(+) B cells do not. (biomedsearch.com)
  • CD5(+) B cells probably play a role in setting up the idiotype network, antigen presentation and tolerance induction. (biomedsearch.com)
  • B cells of most of the chronic lymphoid leukemias express CD5 molecules and, surprisingly, these cells may be expanded in non-organ-specific autoimmune diseases, such as rheumatoid arthritis or primary Sjögren's syndrome. (biomedsearch.com)
  • CD5(+) B cells seems to be involved in the autoantibody production (this does not necessarily imply that pathogenic autoantibodies are produced by CD5(+) B cells) in autoimmune disease and particularly susceptible to transformation in lymphoproliferative disorders. (biomedsearch.com)
  • the VH1 family was significantly diminished in the productive rearrangements of CD5(-) B cells. (nih.gov)
  • 3-23/DP-47 was the most frequently used VH gene segment and was found significantly more often than expected from random usage in productive rearrangements of both CD5(+) and CD5(-) B cells. (nih.gov)
  • Evidence for selection based on the D segment and the JH gene usage was noted in CD5(+) B cells. (nih.gov)
  • Somatically hypermutated VHDJH rearrangements were significantly more frequent and extensive in CD5(-) compared to CD5(+) IgM+ B cells, indicating that IgM+ memory B cells were more frequent in the CD5(-) B cell population. (nih.gov)
  • Of note, the frequency of specific VH genes in the mutated population differed from that in the nonmutated population, suggesting that antigen stimulation imposed additional biases on the repertoire of IgM+ B cells. (nih.gov)
  • Moreover, the influences on the repertoires of CD5(+) and CD5(-) B cells are significantly different, suggesting that human peripheral blood CD5(+) and CD5(-) B cells represent different B cell lineages, with similarities to murine B-1a and B-2 subsets, respectively. (nih.gov)
  • Chimeric antigen receptor (CAR) immunotherapy has recently shown promise in clinical trials for B-cell malignancies, however, designing CARs for T-cell based disease remain a challenge due to the shared surface antigen pool between normal and malignant T-cells. (elsevier.com)
  • Normal T-cells express CD5 but NK (natural killer) cells do not, positioning NK cells as attractive cytotoxicity cells for CD5CAR design. (elsevier.com)
  • In this study investigators are going to attach the CD5 chimeric receptor with CD28 added to it to the patient's T cells. (emilywhiteheadfoundation.org)
  • The cells do not express B-cell lineage restricted antigens or kappa or lambda immunoglobulin light chains or T-cell lineage-restricted antigens. (atcc.org)
  • The cells do express activation antigens. (atcc.org)
  • CD5 plays a role in the interaction of T and B cells and is a counter-receptor for CD72. (miltenyibiotec.com)
  • Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of CD5-PE-Vio770. (miltenyibiotec.com)
  • Rat CD5 derived in Human Cells. (creativebiomart.net)
  • In addition, they express CD5, which is more typically found on T cells. (medscape.com)
  • Because normal CD5 + B cells are present in the mantle zone of lymphoid follicles, B-cell CLL is most likely a malignancy of a mantle zone-based subpopulation of anergic self-reactive cells devoted to the production of polyreactive natural autoantibodies. (medscape.com)
  • B-1 cells, found mainly in the pleural and peritoneal cavities, express high levels of surface IgM, low levels of B220 and IgD, and moderate levels of CD5. (rupress.org)
  • Selection of T cells expressing three distinct transgenic TCRs was also abnormal in CD5-deficient mice. (sciencemag.org)
  • Central and peripheral tolerance prevent autoimmunity by deleting the most aggressive CD8 + T cells but they spare cells that react weakly to tissue-restricted antigen (TRA). (rupress.org)
  • In contrast to high avidity cells, low avidity T cells persist in the antigen-positive periphery with no signs of anergy, unresponsiveness, or prior activation. (rupress.org)
  • Further study found that CD5 and BLNK coexpressed in CLL, but not in normal B- or T cells, are required for STAT3 phosphorylation. (aacrjournals.org)
  • To elucidate the relationship of CD5 and BLNK to CK2 and STAT3, STAT3 was immunoprecipitated from CLL cells, and CK2, CD5, and BLNK were detected in the immunoprecipitate. (aacrjournals.org)
  • CD5 is usually expressed on T cells ( 2 ), although only on a rare B-cell subtype, but not on most B cells ( 3 ). (aacrjournals.org)
  • The current case is unique in its extremely late onset of 17 years and in that two distinct components of lymphoma with different morphology and immunophenotypes coexist in the same isolated location with the conventional diffuse large B-cell lymphoma portion being EBV negative while the anaplastic looking component which expresses T-cells antigens is EBV positive. (hindawi.com)
  • Conclusions These data demonstrate that B cells autoreactive to RBC antigens survive in healthy mice with normal immune systems. (haematologica.org)
  • Exposure to IL-15 and IL-21 enables autoreactive CD8 T cells to respond to weak antigens and cause disease in a mouse model of autoimmune diabetes. (usherbrooke.ca)
  • Mice deficient for the negative regulator CD5 also produced fewer αβ cells, consistent with the model that weak signaling promotes the αβ lineage. (sciencemag.org)
  • CD5 is a pan-T cell surface marker that is also present on a subset of B cells, B-1a cells.Functional and developmental subsets of T cells express characteristic CD5 levels that vary over roughly a 30-fold range. (semanticscholar.org)
  • CRISPR-Cas9 Knock out of CD5 Enhances the Anti-Tumor Activity of Chimeric Antigen Receptor T Cells American Society of Hematology Annual Meeting Virtual (554), 2020. (upenn.edu)
  • Immunohistochemically, the malignant cells expressed markers of B-cell lineage and unusually CD5 positive. (ispub.com)
  • Research has shown that this group of cells are B cells (CD5+CD19+) that express CD5+ [4]. (termedia.pl)
  • CD5+ B cells produce autoantibodies and pro-inflammatory cytokines, increase antigen presentation, and play an important role in autoimmune diseases [5]. (termedia.pl)
  • Based on the production of different cytokines, CD5+B cells are divided into Br1, Br3, and Breg, of which those responsible for the production of interleukin (IL)-10 and for exerting a negative regulatory effect on the immunity are referred to as Br1 (B10) [6, 7]. (termedia.pl)
  • By mutation of all four CD5 intracellular tyrosine residues to phenylalanine, we found the membrane-proximal tyrosine at position 378, which is located in an immunoreceptor tyrosine-based inhibitory (ITIM)-like motif, crucial for SHP-1 association. (nih.gov)
  • CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. (selectscience.net)
  • Intracellular events involved in CD5-induced human T cell activation and proliferation. (abnova.com)
  • These data show that the cellular proteins CK2, CD5, and BLNK are required for constitutive phosphorylation of STAT3 in CLL. (aacrjournals.org)
  • While T-cell antigen receptor (TCR) recognizes antigenic peptides of proteins presented on the self MHC, B-cell antigen receptor (BCR) recognizes essentially any kinds of molecules. (springer.com)
  • Dr. Ruella's laboratory focuses on the mechanisms of relapse after chimeric antigen receptor T cell (CART) immunotherapies with the goal of rationally design innovative combined immunotherapies for relapsing/refractory leukemia and lymphoma. (upenn.edu)
  • Lipid-based antigenic cross-reactivities or molecular mimicry between cellular components and specific microbial antigens may contribute to either pathogenesis of infectious diseases or clearance of cellular lipid products [ 7 , 8 , 15 , 16 , 17 ]. (mdpi.com)
  • found that CD5+CD19+ Bregs secrete IL-10 in peripheral blood of patients with allergic diseases [8]. (termedia.pl)
  • The absence of CD5 rendered thymocytes hyperresponsive to stimulation through the T cell antigen receptor (TCR) in vitro. (sciencemag.org)
  • These observations indicate that CD5 can influence the fate of developing thymocytes by acting as a negative regulator of TCR-mediated signal transduction. (sciencemag.org)
  • Diseases associated with CD5 include Thymus Cancer and Richter's Syndrome . (genecards.org)
  • Diseases associated with CD5 dysfunction include thymus cancer and Richter's Syndrome. (fishersci.com)
  • Additionally, CD5 is highly expressed in T-cell acute lymphoblastic leukemia (T-ALL) and peripheral T-cell lymphomas (PTCLs). (elsevier.com)
  • Although usually strongly CD56+, these lymphomas are negative for other NK cell associated antigens such as CD16 and CD57. (upmc.edu)
  • The occurrence of multicentric conjunctival CD5-positive MALT lymphoma in the younger age further support to the notation that CD5+ MALT-lymphomas arising in the ocular adnexa might be characterized by an unusual clinical course. (ispub.com)
  • Circulating Autoantibodies in Age-Related Macular Degeneration Recognize Human Macular Tissue Antigens Implicated in Autophagy, Immunomodulation, and Protection from Oxidative Stress and Apoptosis. (duke.edu)
  • BACKGROUND: We investigated sera from elderly subjects with and without age-related macular degeneration (AMD) for presence of autoantibodies (AAbs) against human macular antigens and characterized their identity. (duke.edu)
  • 1 However, the frequency of AIHA grossly underestimates the frequency of humoral autoimmunity to RBC antigens, as many anti-RBC autoantibodies do not induce hemolysis, although the reasons for this are not known. (haematologica.org)
  • CD57 is the most commonly expressed cell antigen and CD56 is the least commonly expressed cell antigen. (scielo.br)
  • CD5 is also present on a B lymphocyte subset but is not found on granulocytes or monocytes. (beckman.com)
  • CD5 negatively regulates the T-cell antigen receptor signal transduction pathway: involvement of SH2-containing phosphotyrosine phosphatase SHP-1. (nih.gov)
  • One novel form of anti-T-cell therapy is the immunoconjugate CD5-Plus. (nih.gov)
  • CD5 is a counter-receptor for CD72 and plays a role in the T-B cell interaction. (fishersci.com)
  • These results indicate that the expressed repertoire of IgM+ B cell subsets is shaped by recombinational bias, as well as selection before and after antigen exposure. (nih.gov)
  • CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. (selectscience.net)
  • Finally, confocal microscopy determined that CD5 is cell membrane bound, and fractionation studies revealed that the CK2/CD5/BLNK/STAT3 complex remains in the cytoplasm, whereas serine pSTAT3 is shuttled to the nucleus. (aacrjournals.org)
  • CD5 and cyclin D1 are positive for mantle cell lymphoma). (biocare.net)
  • The relative inefficiency of humoral tolerance to RBC antigens can not be predicted, given the known characteristics of central B-cell tolerance. (haematologica.org)
  • Brummer T, Shaw PE, Reth M, Misawa Y (2002) Inducible gene deletion reveals different roles for B-Raf and Raf-1 in B-cell antigen receptor signalling. (springer.com)
  • 2 The Human Leukocyte Antigen complex (HLA) is one of the inherited factors that influences the development of B-CLL and clinical outcome of diffuse large B-cell lymphoma. (haematologica.org)
  • A total or partial lack of other T-cell antigens, such as CD2, CD5 and CD7, can be observed (3-4). (scielo.br)
  • The pseudo-immunoreceptor tyrosine-based activation motif of CD5 mediates its inhibitory action on B-cell receptor signaling. (semanticscholar.org)
  • Impaired death receptor signaling in leukemia causes antigen-independent resistance by inducing CAR T cell dysfunction. (upenn.edu)
  • The F378 point mutation ablated both SHP-1 binding and the down-regulating activity of CD5 during TCR-CD3 stimulation. (nih.gov)