Substances that are recognized by the immune system and induce an immune reaction.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
Differentiation antigens found on thymocytes and on cytotoxic and suppressor T-lymphocytes. CD8 antigens are members of the immunoglobulin supergene family and are associative recognition elements in MHC (Major Histocompatibility Complex) Class I-restricted interactions.
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
Substances elaborated by bacteria that have antigenic activity.
A bifunctional enzyme that catalyzes the synthesis and HYDROLYSIS of CYCLIC ADP-RIBOSE (cADPR) from NAD+ to ADP-RIBOSE. It is a cell surface molecule which is predominantly expressed on LYMPHOID CELLS and MYELOID CELLS.
Glycoproteins found on immature hematopoietic cells and endothelial cells. They are the only molecules to date whose expression within the blood system is restricted to a small number of progenitor cells in the bone marrow.
Differentiation antigens expressed on B-lymphocytes and B-cell precursors. They are involved in regulation of B-cell proliferation.
A member of the tumor necrosis factor receptor superfamily with specificity for CD40 LIGAND. It is found on mature B-LYMPHOCYTES and some EPITHELIAL CELLS, lymphoid DENDRITIC CELLS. Evidence suggests that CD40-dependent activation of B-cells is important for generation of memory B-cells within the germinal centers. Mutations of the gene for CD40 antigen result in HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 3. Signaling of the receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
A membrane glycoprotein and differentiation antigen expressed on the surface of T-cells that binds to CD40 ANTIGENS on B-LYMPHOCYTES and induces their proliferation. Mutation of the gene for CD40 ligand is a cause of HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 1.
Unglycosylated phosphoproteins expressed only on B-cells. They are regulators of transmembrane Ca2+ conductance and thought to play a role in B-cell activation and proliferation.
Substances elaborated by viruses that have antigenic activity.
Costimulatory T-LYMPHOCYTE receptors that have specificity for CD80 ANTIGEN and CD86 ANTIGEN. Activation of this receptor results in increased T-cell proliferation, cytokine production and promotion of T-cell survival.
Acidic sulfated integral membrane glycoproteins expressed in several alternatively spliced and variable glycosylated forms on a wide variety of cell types including mature T-cells, B-cells, medullary thymocytes, granulocytes, macrophages, erythrocytes, and fibroblasts. CD44 antigens are the principle cell surface receptors for hyaluronate and this interaction mediates binding of lymphocytes to high endothelial venules. (From Abbas et al., Cellular and Molecular Immunology, 2d ed, p156)
Differentiation antigens expressed on pluripotential hematopoietic cells, most human thymocytes, and a major subset of peripheral blood T-lymphocytes. They have been implicated in integrin-mediated cellular adhesion and as signalling receptors on T-cells.
Glycolipid-anchored membrane glycoproteins expressed on cells of the myelomonocyte lineage including monocytes, macrophages, and some granulocytes. They function as receptors for the complex of lipopolysaccharide (LPS) and LPS-binding protein.
Glycoprotein members of the immunoglobulin superfamily which participate in T-cell adhesion and activation. They are expressed on most peripheral T-lymphocytes, natural killer cells, and thymocytes, and function as co-receptors or accessory molecules in the T-cell receptor complex.
Ratio of T-LYMPHOCYTES that express the CD4 ANTIGEN to those that express the CD8 ANTIGEN. This value is commonly assessed in the diagnosis and staging of diseases affecting the IMMUNE SYSTEM including HIV INFECTIONS.
Glycoproteins expressed on all mature T-cells, thymocytes, and a subset of mature B-cells. Antibodies specific for CD5 can enhance T-cell receptor-mediated T-cell activation. The B-cell-specific molecule CD72 is a natural ligand for CD5. (From Abbas et al., Cellular and Molecular Immunology, 2d ed, p156)
Antigens expressed primarily on the membranes of living cells during sequential stages of maturation and differentiation. As immunologic markers they have high organ and tissue specificity and are useful as probes in studies of normal cell development as well as neoplastic transformation.
A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.
Glycoproteins expressed on cortical thymocytes and on some dendritic cells and B-cells. Their structure is similar to that of MHC Class I and their function has been postulated as similar also. CD1 antigens are highly specific markers for human LANGERHANS CELLS.
Antibodies produced by a single clone of cells.
The 140 kDa isoform of NCAM (neural cell adhesion molecule) containing a transmembrane domain and short cytoplasmic tail. It is expressed by all lymphocytes mediating non-MHC restricted cytotoxicity and is present on some neural tissues and tumors.
Antigens expressed on the cell membrane of T-lymphocytes during differentiation, activation, and normal and neoplastic transformation. Their phenotypic characterization is important in differential diagnosis and studies of thymic ontogeny and T-cell function.
A membrane-bound or cytosolic enzyme that catalyzes the synthesis of CYCLIC ADP-RIBOSE (cADPR) from nicotinamide adenine dinucleotide (NAD). This enzyme generally catalyzes the hydrolysis of cADPR to ADP-RIBOSE, as well, and sometimes the synthesis of cyclic ADP-ribose 2' phosphate (2'-P-cADPR) from NADP.
Surface antigens expressed on myeloid cells of the granulocyte-monocyte-histiocyte series during differentiation. Analysis of their reactivity in normal and malignant myelomonocytic cells is useful in identifying and classifying human leukemias and lymphomas.
A costimulatory ligand expressed by ANTIGEN-PRESENTING CELLS that binds to CTLA-4 ANTIGEN with high specificity and to CD28 ANTIGEN with low specificity. The interaction of CD80 with CD28 ANTIGEN provides a costimulatory signal to T-LYMPHOCYTES, while its interaction with CTLA-4 ANTIGEN may play a role in inducing PERIPHERAL TOLERANCE.
Tetraspanin proteins found at high levels in cells of the lymphoid-myeloid lineage. CD53 antigens may be involved regulating the differentiation of T-LYMPHOCYTES and the activation of B-LYMPHOCYTES.
A cell adhesion protein that was originally identified as a heat stable antigen in mice. It is involved in METASTASIS and is highly expressed in many NEOPLASMS.
Zinc-binding metalloproteases that are members of the type II integral membrane metalloproteases. They are expressed by GRANULOCYTES; MONOCYTES; and their precursors as well as by various non-hematopoietic cells. They release an N-terminal amino acid from a peptide, amide or arylamide.
Any part or derivative of any protozoan that elicits immunity; malaria (Plasmodium) and trypanosome antigens are presently the most frequently encountered.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
A costimulatory ligand expressed by ANTIGEN-PRESENTING CELLS that binds to CD28 ANTIGEN with high specificity and to CTLA-4 ANTIGEN with low specificity. The interaction of CD86 with CD28 ANTIGEN provides a stimulatory signal to T-LYMPHOCYTES, while its interaction with CTLA-4 ANTIGEN may play a role in inducing PERIPHERAL TOLERANCE.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.
A tumor necrosis factor receptor subtype found in a variety of tissues and on activated LYMPHOCYTES. It has specificity for FAS LIGAND and plays a role in regulation of peripheral immune responses and APOPTOSIS. Multiple isoforms of the protein exist due to multiple ALTERNATIVE SPLICING. The activated receptor signals via a conserved death domain that associates with specific TNF RECEPTOR-ASSOCIATED FACTORS in the CYTOPLASM.
Antigens determined by leukocyte loci found on chromosome 6, the major histocompatibility loci in humans. They are polypeptides or glycoproteins found on most nucleated cells and platelets, determine tissue types for transplantation, and are associated with certain diseases.
Membrane antigens associated with maturation stages of B-lymphocytes, often expressed in tumors of B-cell origin.
High-molecular weight glycoproteins uniquely expressed on the surface of LEUKOCYTES and their hemopoietic progenitors. They contain a cytoplasmic protein tyrosine phosphatase activity which plays a role in intracellular signaling from the CELL SURFACE RECEPTORS. The CD45 antigens occur as multiple isoforms that result from alternative mRNA splicing and differential usage of three exons.
Process of classifying cells of the immune system based on structural and functional differences. The process is commonly used to analyze and sort T-lymphocytes into subsets based on CD antigens by the technique of flow cytometry.
Substances of fungal origin that have antigenic activity.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The major group of transplantation antigens in the mouse.
A 67-kDa sialic acid binding lectin that is specific for MYELOID CELLS and MONOCYTE-MACROPHAGE PRECURSOR CELLS. This protein is the smallest siglec subtype and contains a single immunoglobulin C2-set domain. It may play a role in intracellular signaling via its interaction with SHP-1 PROTEIN-TYROSINE PHOSPHATASE and SHP-2 PROTEIN-TYROSINE PHOSPHATASE.
Any part or derivative of a helminth that elicits an immune reaction. The most commonly seen helminth antigens are those of the schistosomes.
Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.
Cell-surface glycoprotein beta-chains that are non-covalently linked to specific alpha-chains of the CD11 family of leukocyte-adhesion molecules (RECEPTORS, LEUKOCYTE-ADHESION). A defect in the gene encoding CD18 causes LEUKOCYTE-ADHESION DEFICIENCY SYNDROME.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
A member of the tumor necrosis factor receptor superfamily that may play a role in the regulation of NF-KAPPA B and APOPTOSIS. They are found on activated T-LYMPHOCYTES; B-LYMPHOCYTES; NEUTROPHILS; EOSINOPHILS; MAST CELLS and NK CELLS. Overexpression of CD30 antigen in hematopoietic malignancies make the antigen clinically useful as a biological tumor marker. Signaling of the receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
Glycoproteins found on the membrane or surface of cells.
A critical subpopulation of regulatory T-lymphocytes involved in MHC Class I-restricted interactions. They include both cytotoxic T-lymphocytes (T-LYMPHOCYTES, CYTOTOXIC) and CD8+ suppressor T-lymphocytes.
Sites on an antigen that interact with specific antibodies.
A subtype of tetraspanin proteins that play a role in cell adhesion, cell motility, and tumor metastasis. CD9 antigens take part in the process of platelet activation and aggregation, the formation of paranodal junctions in neuronal tissue, and the fusion of sperm with egg.
A glycoprotein that is secreted into the luminal surface of the epithelia in the gastrointestinal tract. It is found in the feces and pancreaticobiliary secretions and is used to monitor the response to colon cancer treatment.
A subclass of HLA-D antigens that consist of alpha and beta chains. The inheritance of HLA-DR antigens differs from that of the HLA-DQ ANTIGENS and HLA-DP ANTIGENS.
A trisaccharide antigen expressed on glycolipids and many cell-surface glycoproteins. In the blood the antigen is found on the surface of NEUTROPHILS; EOSINOPHILS; and MONOCYTES. In addition, CD15 antigen is a stage-specific embryonic antigen.
Those proteins recognized by antibodies from serum of animals bearing tumors induced by viruses; these proteins are presumably coded for by the nucleic acids of the same viruses that caused the neoplastic transformation.
Established cell cultures that have the potential to propagate indefinitely.
A sialic acid-rich protein and an integral cell membrane mucin. It plays an important role in activation of T-LYMPHOCYTES.
Leukocyte differentiation antigens and major platelet membrane glycoproteins present on MONOCYTES; ENDOTHELIAL CELLS; PLATELETS; and mammary EPITHELIAL CELLS. They play major roles in CELL ADHESION; SIGNAL TRANSDUCTION; and regulation of angiogenesis. CD36 is a receptor for THROMBOSPONDINS and can act as a scavenger receptor that recognizes and transports oxidized LIPOPROTEINS and FATTY ACIDS.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A group of three different alpha chains (CD11a, CD11b, CD11c) that are associated with an invariant CD18 beta chain (ANTIGENS, CD18). The three resulting leukocyte-adhesion molecules (RECEPTORS, LEUKOCYTE ADHESION) are LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN-1; MACROPHAGE-1 ANTIGEN; and ANTIGEN, P150,95.
Large, transmembrane, non-covalently linked glycoproteins (alpha and beta). Both chains can be polymorphic although there is more structural variation in the beta chains. The class II antigens in humans are called HLA-D ANTIGENS and are coded by a gene on chromosome 6. In mice, two genes named IA and IE on chromosome 17 code for the H-2 antigens. The antigens are found on B-lymphocytes, macrophages, epidermal cells, and sperm and are thought to mediate the competence of and cellular cooperation in the immune response. The term IA antigens used to refer only to the proteins encoded by the IA genes in the mouse, but is now used as a generic term for any class II histocompatibility antigen.
A group of antigens that includes both the major and minor histocompatibility antigens. The former are genetically determined by the major histocompatibility complex. They determine tissue type for transplantation and cause allograft rejections. The latter are systems of allelic alloantigens that can cause weak transplant rejection.
Small glycoproteins found on both hematopoietic and non-hematopoietic cells. CD59 restricts the cytolytic activity of homologous complement by binding to C8 and C9 and blocking the assembly of the membrane attack complex. (From Barclay et al., The Leukocyte Antigen FactsBook, 1993, p234)
IMMUNOGLOBULINS on the surface of B-LYMPHOCYTES. Their MESSENGER RNA contains an EXON with a membrane spanning sequence, producing immunoglobulins in the form of type I transmembrane proteins as opposed to secreted immunoglobulins (ANTIBODIES) which do not contain the membrane spanning segment.
Nuclear antigen with a role in DNA synthesis, DNA repair, and cell cycle progression. PCNA is required for the coordinated synthesis of both leading and lagging strands at the replication fork during DNA replication. PCNA expression correlates with the proliferation activity of several malignant and non-malignant cell types.
Oligosaccharide antigenic determinants found principally on NK cells and T-cells. Their role in the immune response is poorly understood.
A transmembrane protein belonging to the tumor necrosis factor superfamily that specifically binds to CD27 ANTIGEN. It is found on activated T-LYMPHOCYTES; B-LYMPHOCYTES; and DENDRITIC CELLS where it plays a role in stimulating the proliferation of CD4-POSITIVE T-LYMPHOCYTES and CD8-POSITIVE T-LYMPHOCYTES.
A ubiquitously expressed complement receptor that binds COMPLEMENT C3B and COMPLEMENT C4B and serves as a cofactor for their inactivation. CD46 also interacts with a wide variety of pathogens and mediates immune response.
A class of animal lectins that bind to carbohydrate in a calcium-dependent manner. They share a common carbohydrate-binding domain that is structurally distinct from other classes of lectins.
Glycoproteins with a wide distribution on hematopoietic and non-hematopoietic cells and strongly expressed on macrophages. CD58 mediates cell adhesion by binding to CD2; (ANTIGENS, CD2); and this enhances antigen-specific T-cell activation.
55-kDa antigens found on HELPER-INDUCER T-LYMPHOCYTES and on a variety of other immune cell types. CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in MAJOR HISTOCOMPATIBILITY COMPLEX class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as INTERLEUKIN-15 receptors and bind to the HIV receptors, binding directly to the HIV ENVELOPE PROTEIN GP120.
A ubiquitously expressed membrane glycoprotein. It interacts with a variety of INTEGRINS and mediates responses to EXTRACELLULAR MATRIX PROTEINS.
A CD antigen that contains a conserved I domain which is involved in ligand binding. When combined with CD18 the two subunits form MACROPHAGE-1 ANTIGEN.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A glycoprotein that is a kallikrein-like serine proteinase and an esterase, produced by epithelial cells of both normal and malignant prostate tissue. It is an important marker for the diagnosis of prostate cancer.
An integrin alpha subunit of approximately 150-kDa molecular weight. It is expressed at high levels on monocytes and combines with CD18 ANTIGEN to form the cell surface receptor INTEGRIN ALPHAXBETA2. The subunit contains a conserved I-domain which is characteristic of several of alpha integrins.
The lipopolysaccharide-protein somatic antigens, usually from gram-negative bacteria, important in the serological classification of enteric bacilli. The O-specific chains determine the specificity of the O antigens of a given serotype. O antigens are the immunodominant part of the lipopolysaccharide molecule in the intact bacterial cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
A specific HLA-A surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-A*02 allele family.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Progenitor cells from which all blood cells derive.
The number of CD4-POSITIVE T-LYMPHOCYTES per unit volume of BLOOD. Determination requires the use of a fluorescence-activated flow cytometer.
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
Carbohydrate antigens expressed by malignant tissue. They are useful as tumor markers and are measured in the serum by means of a radioimmunoassay employing monoclonal antibodies.
GPI-linked membrane proteins broadly distributed among hematopoietic and non-hematopoietic cells. CD55 prevents the assembly of C3 CONVERTASE or accelerates the disassembly of preformed convertase, thus blocking the formation of the membrane attack complex.
Cell adhesion molecules present on virtually all monocytes, platelets, and granulocytes. CD31 is highly expressed on endothelial cells and concentrated at the junctions between them.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Membrane glycoproteins consisting of an alpha subunit and a BETA 2-MICROGLOBULIN beta subunit. In humans, highly polymorphic genes on CHROMOSOME 6 encode the alpha subunits of class I antigens and play an important role in determining the serological specificity of the surface antigen. Class I antigens are found on most nucleated cells and are generally detected by their reactivity with alloantisera. These antigens are recognized during GRAFT REJECTION and restrict cell-mediated lysis of virus-infected cells.
Tetraspanin proteins that are involved in a variety of cellular functions including BASEMENT MEMBRANE assembly, and in the formation of a molecular complexes on the surface of LYMPHOCYTES.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
A member of the tumor necrosis factor receptor superfamily that is specific for 4-1BB LIGAND. It is found in a variety of immune cell types including activated T-LYMPHOCYTES; NATURAL KILLER CELLS; and DENDRITIC CELLS. Activation of the receptor on T-LYMPHOCYTES plays a role in their expansion, production of cytokines and survival. Signaling by the activated receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Proteins prepared by recombinant DNA technology.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.
Polymorphic class I human histocompatibility (HLA) surface antigens present on almost all nucleated cells. At least 20 antigens have been identified which are encoded by the A locus of multiple alleles on chromosome 6. They serve as targets for T-cell cytolytic responses and are involved with acceptance or rejection of tissue/organ grafts.
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).
Receptors present on activated T-LYMPHOCYTES and B-LYMPHOCYTES that are specific for INTERLEUKIN-2 and play an important role in LYMPHOCYTE ACTIVATION. They are heterotrimeric proteins consisting of the INTERLEUKIN-2 RECEPTOR ALPHA SUBUNIT, the INTERLEUKIN-2 RECEPTOR BETA SUBUNIT, and the INTERLEUKIN RECEPTOR COMMON GAMMA-CHAIN.
Sets of cell surface antigens located on BLOOD CELLS. They are usually membrane GLYCOPROTEINS or GLYCOLIPIDS that are antigenically distinguished by their carbohydrate moieties.
Those hepatitis B antigens found on the surface of the Dane particle and on the 20 nm spherical and tubular particles. Several subspecificities of the surface antigen are known. These were formerly called the Australia antigen.
Ubiquitously-expressed tetraspanin proteins that are found in late ENDOSOMES and LYSOSOMES and have been implicated in intracellular transport of proteins.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Tetraspanin proteins found associated with LAMININ-binding INTEGRINS. The CD151 antigens may play a role in the regulation of CELL MOTILITY.
A component of the B-cell antigen receptor that is involved in B-cell antigen receptor heavy chain transport to the PLASMA MEMBRANE. It is expressed almost exclusively in B-LYMPHOCYTES and serves as a useful marker for B-cell NEOPLASMS.
An encapsulated lymphatic organ through which venous blood filters.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Human immune-response or Class II antigens found mainly, but not exclusively, on B-lymphocytes and produced from genes of the HLA-D locus. They are extremely polymorphic families of glycopeptides, each consisting of two chains, alpha and beta. This group of antigens includes the -DR, -DQ and -DP designations, of which HLA-DR is most studied; some of these glycoproteins are associated with certain diseases, possibly of immune etiology.
A membrane-bound tumor necrosis family member found primarily on activated T-LYMPHOCYTES that binds specifically to CD30 ANTIGEN. It may play a role in INFLAMMATION and immune regulation.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
A class of enzymes involved in the hydrolysis of the N-glycosidic bond of nitrogen-linked sugars.
A form of undifferentiated malignant LYMPHOMA usually found in central Africa, but also reported in other parts of the world. It is commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. B-cell antigens are expressed on the immature cells that make up the tumor in virtually all cases of Burkitt lymphoma. The Epstein-Barr virus (HERPESVIRUS 4, HUMAN) has been isolated from Burkitt lymphoma cases in Africa and it is implicated as the causative agent in these cases; however, most non-African cases are EBV-negative.
Molecules on the surface of B- and T-lymphocytes that recognize and combine with specific antigens.
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.
An alpha-integrin subunit found on lymphocytes, granulocytes, macrophages and monocytes. It combines with the integrin beta2 subunit (CD18 ANTIGEN) to form LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN-1.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Antigens of the virion of the HEPATITIS B VIRUS or the Dane particle, its surface (HEPATITIS B SURFACE ANTIGENS), core (HEPATITIS B CORE ANTIGENS), and other associated antigens, including the HEPATITIS B E ANTIGENS.
The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.
The processes triggered by interactions of ANTIBODIES with their ANTIGENS.
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
Mice homozygous for the mutant autosomal recessive gene "scid" which is located on the centromeric end of chromosome 16. These mice lack mature, functional lymphocytes and are thus highly susceptible to lethal opportunistic infections if not chronically treated with antibiotics. The lack of B- and T-cell immunity resembles severe combined immunodeficiency (SCID) syndrome in human infants. SCID mice are useful as animal models since they are receptive to implantation of a human immune system producing SCID-human (SCID-hu) hematochimeric mice.
Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
A heterogeneous group of immunocompetent cells that mediate the cellular immune response by processing and presenting antigens to the T-cells. Traditional antigen-presenting cells include MACROPHAGES; DENDRITIC CELLS; LANGERHANS CELLS; and B-LYMPHOCYTES. FOLLICULAR DENDRITIC CELLS are not traditional antigen-presenting cells, but because they hold antigen on their cell surface in the form of IMMUNE COMPLEXES for B-cell recognition they are considered so by some authors.
The type species of LYMPHOCRYPTOVIRUS, subfamily GAMMAHERPESVIRINAE, infecting B-cells in humans. It is thought to be the causative agent of INFECTIOUS MONONUCLEOSIS and is strongly associated with oral hairy leukoplakia (LEUKOPLAKIA, HAIRY;), BURKITT LYMPHOMA; and other malignancies.
T-cell receptors composed of CD3-associated alpha and beta polypeptide chains and expressed primarily in CD4+ or CD8+ T-cells. Unlike immunoglobulins, the alpha-beta T-cell receptors recognize antigens only when presented in association with major histocompatibility (MHC) molecules.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Class I human histocompatibility (HLA) surface antigens encoded by more than 30 detectable alleles on locus B of the HLA complex, the most polymorphic of all the HLA specificities. Several of these antigens (e.g., HLA-B27, -B7, -B8) are strongly associated with predisposition to rheumatoid and other autoimmune disorders. Like other class I HLA determinants, they are involved in the cellular immune reactivity of cytolytic T lymphocytes.
The altered state of immunologic responsiveness resulting from initial contact with antigen, which enables the individual to produce antibodies more rapidly and in greater quantity in response to secondary antigenic stimulus.
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
The phenomenon of target cell destruction by immunologically active effector cells. It may be brought about directly by sensitized T-lymphocytes or by lymphoid or myeloid "killer" cells, or it may be mediated by cytotoxic antibody, cytotoxic factor released by lymphoid cells, or complement.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
A melanosome-specific protein that plays a role in the expression, stability, trafficking, and processing of GP100 MELANOMA ANTIGEN, which is critical to the formation of Stage II MELANOSOMES. The protein is used as an antigen marker for MELANOMA cells.
A widely distributed cell surface transmembrane glycoprotein that stimulates the synthesis of MATRIX METALLOPROTEINASES. It is found at high levels on the surface of malignant NEOPLASMS and may play a role as a mediator of malignant cell behavior.
A general term for various neoplastic diseases of the lymphoid tissue.
An albumin obtained from the white of eggs. It is a member of the serpin superfamily.
Antigens associated with specific proteins of the human adult T-cell immunodeficiency virus (HIV); also called HTLV-III-associated and lymphadenopathy-associated virus (LAV) antigens.
An inhibitory T CELL receptor that is closely related to CD28 ANTIGEN. It has specificity for CD80 ANTIGEN and CD86 ANTIGEN and acts as a negative regulator of peripheral T cell function. CTLA-4 antigen is believed to play role in inducing PERIPHERAL TOLERANCE.
A promyelocytic cell line derived from a patient with ACUTE PROMYELOCYTIC LEUKEMIA. HL-60 cells lack specific markers for LYMPHOID CELLS but express surface receptors for FC FRAGMENTS and COMPLEMENT SYSTEM PROTEINS. They also exhibit phagocytic activity and responsiveness to chemotactic stimuli. (From Hay et al., American Type Culture Collection, 7th ed, pp127-8)
A widely expressed transmembrane glycoprotein that functions as a METASTASIS suppressor protein. It is underexpressed in a variety of human NEOPLASMS.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
A group of differentiation surface antigens, among the first to be discovered on thymocytes and T-lymphocytes. Originally identified in the mouse, they are also found in other species including humans, and are expressed on brain neurons and other cells.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
The specific failure of a normally responsive individual to make an immune response to a known antigen. It results from previous contact with the antigen by an immunologically immature individual (fetus or neonate) or by an adult exposed to extreme high-dose or low-dose antigen, or by exposure to radiation, antimetabolites, antilymphocytic serum, etc.
Manifestations of the immune response which are mediated by antigen-sensitized T-lymphocytes via lymphokines or direct cytotoxicity. This takes place in the absence of circulating antibody or where antibody plays a subordinate role.
A single, unpaired primary lymphoid organ situated in the MEDIASTINUM, extending superiorly into the neck to the lower edge of the THYROID GLAND and inferiorly to the fourth costal cartilage. It is necessary for normal development of immunologic function early in life. By puberty, it begins to involute and much of the tissue is replaced by fat.
Endogenous tissue constituents that have the ability to interact with AUTOANTIBODIES and cause an immune response.
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
Nuclear antigens encoded by VIRAL GENES found in HUMAN HERPESVIRUS 4. At least six nuclear antigens have been identified.
A soluble substance elaborated by antigen- or mitogen-stimulated T-LYMPHOCYTES which induces DNA synthesis in naive lymphocytes.
A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.
A cell line derived from cultured tumor cells.
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
A sex-specific cell surface antigen produced by the sex-determining gene of the Y chromosome in mammals. It causes syngeneic grafts from males to females to be rejected and interacts with somatic elements of the embryologic undifferentiated gonad to produce testicular organogenesis.
A cell adhesion molecule of the immunoglobulin superfamily that is expressed in ENDOTHELIAL CELLS and is involved in INTERCELLULAR JUNCTIONS.
Antigens stimulating the formation of, or combining with heterophile antibodies. They are cross-reacting antigens found in phylogenetically unrelated species.
CD4-positive T cells that inhibit immunopathology or autoimmune disease in vivo. They inhibit the immune response by influencing the activity of other cell types. Regulatory T-cells include naturally occurring CD4+CD25+ cells, IL-10 secreting Tr1 cells, and Th3 cells.
Antibodies obtained from a single clone of cells grown in mice or rats.
Antigenic determinants recognized and bound by the T-cell receptor. Epitopes recognized by the T-cell receptor are often located in the inner, unexposed side of the antigen, and become accessible to the T-cell receptors after proteolytic processing of the antigen.
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
A heterodimeric protein that is a cell surface antigen associated with lymphocyte activation. The initial characterization of this protein revealed one identifiable heavy chain (ANTIGENS, CD98 HEAVY CHAIN) and an indeterminate smaller light chain. It is now known that a variety of light chain subunits (ANTIGENS, CD98 LIGHT CHAINS) can dimerize with the heavy chain. Depending upon its light chain composition a diverse array of functions can be found for this protein. Functions include: type L amino acid transport, type y+L amino acid transport and regulation of cellular fusion.
The hepatitis B antigen within the core of the Dane particle, the infectious hepatitis virion.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
The complex formed by the binding of antigen and antibody molecules. The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES.
They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system.
The sum of the weight of all the atoms in a molecule.
Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.
A group of the D-related HLA antigens found to differ from the DR antigens in genetic locus and therefore inheritance. These antigens are polymorphic glycoproteins comprising alpha and beta chains and are found on lymphoid and other cells, often associated with certain diseases.
Immunoglobulins produced in response to VIRAL ANTIGENS.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
A glycolipid, cross-species antigen that induces production of antisheep hemolysin. It is present on the tissue cells of many species but absent in humans. It is found in many infectious agents.
Elements of limited time intervals, contributing to particular results or situations.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
An inhibitory B7 antigen that has specificity for the T-CELL receptor PROGRAMMED CELL DEATH 1 PROTEIN. CD274 antigen provides negative signals that control and inhibit T-cell responses and is found at higher than normal levels on tumor cells, suggesting its potential role in TUMOR IMMUNE EVASION.
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
A species of POLYOMAVIRUS originally isolated from Rhesus monkey kidney tissue. It produces malignancy in human and newborn hamster kidney cell cultures.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
Substances that augment, stimulate, activate, potentiate, or modulate the immune response at either the cellular or humoral level. The classical agents (Freund's adjuvant, BCG, Corynebacterium parvum, et al.) contain bacterial antigens. Some are endogenous (e.g., histamine, interferon, transfer factor, tuftsin, interleukin-1). Their mode of action is either non-specific, resulting in increased immune responsiveness to a wide variety of antigens, or antigen-specific, i.e., affecting a restricted type of immune response to a narrow group of antigens. The therapeutic efficacy of many biological response modifiers is related to their antigen-specific immunoadjuvanticity.
Antigens that exist in alternative (allelic) forms in a single species. When an isoantigen is encountered by species members who lack it, an immune response is induced. Typical isoantigens are the BLOOD GROUP ANTIGENS.
Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell.
A melanosome-associated protein that plays a role in the maturation of the MELANOSOME.
The genetic region which contains the loci of genes which determine the structure of the serologically defined (SD) and lymphocyte-defined (LD) TRANSPLANTATION ANTIGENS, genes which control the structure of the IMMUNE RESPONSE-ASSOCIATED ANTIGENS, HUMAN; the IMMUNE RESPONSE GENES which control the ability of an animal to respond immunologically to antigenic stimuli, and genes which determine the structure and/or level of the first four components of complement.
Bone marrow-derived lymphocytes that possess cytotoxic properties, classically directed against transformed and virus-infected cells. Unlike T CELLS; and B CELLS; NK CELLS are not antigen specific. The cytotoxicity of natural killer cells is determined by the collective signaling of an array of inhibitory and stimulatory CELL SURFACE RECEPTORS. A subset of T-LYMPHOCYTES referred to as NATURAL KILLER T CELLS shares some of the properties of this cell type.
A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
Form of passive immunization where previously sensitized immunologic agents (cells or serum) are transferred to non-immune recipients. When transfer of cells is used as a therapy for the treatment of neoplasms, it is called adoptive immunotherapy (IMMUNOTHERAPY, ADOPTIVE).

Adenoviral gene transfer into the normal and injured spinal cord: enhanced transgene stability by combined administration of temperature-sensitive virus and transient immune blockade. (1/2721)

This study characterized gene transfer into both normal and injured adult rat dorsal spinal cord using first (E1-/E3-) or second (E1-/E2A125/E3-, temperature-sensitive; ts) generation of replication-defective adenoviral (Ad) vectors. A novel immunosuppressive regimen aimed at blocking CD4/CD45 lymphocytic receptors was tested for improving transgene persistence. In addition, the effect of gene transfer on nociception was also evaluated. Seven days after treatment, numerous LacZ-positive cells were observed after transfection with either viral vector. By 21 days after transfection, beta-galactosidase staining was reduced and suggestive of ongoing cytopathology in both Ad-treated groups, despite the fact that the immunogenicity of LacZ/Adts appeared less when compared with that elicited by the LacZ/Ad vector. In contrast, immunosuppressed animals showed a significant (P < or = 0.05) increase in the number of LacZ-positive cells not displaying cytopathology. In these animals, a concomitant reduction in numbers of macrophages/microglia and CD4 and CD8 lymphocytes was observed. Only animals that received LacZ/Adts and immunosuppression showed transgene expression after 60 days. Similar results were observed in animals in which the L4-L5 dorsal roots were lesioned before transfection. Gene transfer into the dorsal spinal cord did not affect nociception, independent of the adenovirus vector. These results indicate that immune blockade of the CD4/CD45 lymphocytic receptors enhanced transgene stability in adult animals with normal or injured spinal cords and that persistent transgene expression in the spinal cord does not interfere with normal neural function.  (+info)

Oval cell numbers in human chronic liver diseases are directly related to disease severity. (2/2721)

The risk of developing hepatocellular carcinoma is significantly increased in patients with genetic hemochromatosis, alcoholic liver disease, or chronic hepatitis C infection. The precise mechanisms underlying the development of hepatocellular carcinoma in these conditions are not well understood. Stem cells within the liver, termed oval cells, are involved in the pathogenesis of hepatocellular carcinoma in animal models and may be important in the development of hepatocellular carcinoma in human chronic liver diseases. The aims of this study were to determine whether oval cells could be detected in the liver of patients with genetic hemochromatosis, alcoholic liver disease, or chronic hepatitis C, and whether there is a relationship between the severity of the liver disease and the number of oval cells. Oval cells were detected using histology and immunohistochemistry in liver biopsies from patients with genetic hemochromatosis, alcoholic liver disease, or chronic hepatitis C. Oval cells were not observed in normal liver controls. Oval cell numbers increased significantly with the progression of disease severity from mild to severe in each of the diseases studied. We conclude that oval cells are frequently found in subjects with genetic hemochromatosis, alcoholic liver disease, or chronic hepatitis C. There is an association between severity of liver disease and increase in the number of oval cells consistent with the hypothesis that oval cell proliferation is associated with increased risk for development of hepatocellular carcinoma in chronic liver disease.  (+info)

Peripheral autoantigen induces regulatory T cells that prevent autoimmunity. (3/2721)

Previous studies have shown that autoimmune thyroiditis can be induced in normal laboratory rats after thymectomy and split dose gamma-irradiation. Development of disease can be prevented by reconstitution of PVG rats shortly after their final irradiation with either peripheral CD4(+)CD45RC- T cells or CD4(+)CD8(-) thymocytes from syngeneic donors. Although the activity of both populations is known to depend on the activities of endogenously produced interleukin 4 and transforming growth factor beta, implying a common mechanism, the issue of antigen specificity of the cells involved has not yet been addressed. In this study, we show that the regulatory T cells that prevent autoimmune thyroiditis are generated in vivo only when the relevant autoantigen is also present. Peripheral CD4(+) T cells, from rats whose thyroids were ablated in utero by treatment with 131I, were unable to prevent disease development upon adoptive transfer into thymectomized and irradiated recipients. This regulatory deficit is specific for thyroid autoimmunity, since CD4(+) T cells from 131I-treated PVG.RT1(u) rats were as effective as those from normal donors at preventing diabetes in thymectomized and irradiated PVG.RT1(u) rats. Significantly, in contrast to the peripheral CD4(+) T cells, CD4(+)CD8(-) thymocytes from 131I-treated PVG donors were still able to prevent thyroiditis upon adoptive transfer. Taken together, these data indicate that it is the peripheral autoantigen itself that stimulates the generation of the appropriate regulatory cells from thymic emigrant precursors.  (+info)

Selective recruitment of CCR4-bearing Th2 cells toward antigen-presenting cells by the CC chemokines thymus and activation-regulated chemokine and macrophage-derived chemokine. (4/2721)

Helper T cells are classified into Th1 and Th2 subsets based on their profiles of cytokine production. Th1 cells are involved in cell-mediated immunity, whereas Th2 cells induce humoral responses. Selective recruitment of these two subsets depends on specific adhesion molecules and specific chemoattractants. Here, we demonstrate that the T cell-directed CC chemokine thymus and activation-regulated chemokine (TARC) was abundantly produced by monocytes treated with granulocyte macrophage colony stimulating factor (GM-CSF) or IL-3, especially in the presence of IL-4 and by dendritic cells derived from monocytes cultured with GM-CSF + IL-4. The receptor for TARC and another macrophage/dendritic cell-derived CC chemokine macrophage-derived chemokine (MDC) is CCR4, a G protein-coupled receptor. CCR4 was found to be expressed on approximately 20% of adult peripheral blood effector/memory CD4+ T cells. T cells attracted by TARC and MDC generated cell lines predominantly producing Th2-type cytokines, IL-4 and IL-5. Fractionated CCR4+ cells but not CCR4- cells also selectively gave rise to Th2-type cell lines. When naive CD4+ T cells from adult peripheral blood were polarized in vitro, Th2-type cells selectively expressed CCR4 and vigorously migrated toward TARC and MDC. Taken together, CCR4 is selectively expressed on Th2-type T cells and antigen-presenting cells may recruit Th2 cells expressing CCR4 by producing TARC and MDC in Th2-dominant conditions.  (+info)

CD45 can act as a negative regulator for the transition from early to late CD4+ CD8+ thymocytes. (5/2721)

The differentiation process from CD4-CD8- double-negative (DN) thymocytes to CD4+CD8+ double-positive (DP) stage is accompanied by vigorous proliferation. The resulting DP cells contain a sizable proportion of large cycling cells, but most DP cells are small resting cells. To explore the molecular mechanisms which regulate cell proliferation of DP thymocytes prior to further development, we used TCR-transgenic (Tg) mice with non-selecting MHC (Tg-Neut), which contain almost exclusively DP thymocytes that are not subject to either positive or negative selection. In Tg-Neut, the thymus contained DP cells of relatively large size, which showed higher extracellular signal-regulated kinase activity and enhanced responsiveness to mitogen compared to small DP cells. This indicates that all the large DP cells in the thymus are not positively selected and that they possess proliferative potential. When Tg-Neut mice were backcrossed with CD45 knockout mice (CD454-/- Tg-Neut), the thymus showed an increase of large DP cells and cycling cells, but a decrease of apoptotic cells. Furthermore, Bcl-2 expression and Jun N-terminal kinase activity, which are associated with resistance to apoptosis, were enhanced. These observations suggest that thymocyte proliferation in the DP stage is suppressed by a CD45-related process with regulation of mitogen-activated protein kinase and Bcl-2 unless DP cells receive TCR-mediated signals.  (+info)

Early ontogeny of monocytes and macrophages in the pig. (6/2721)

Prenatal development of cord blood monocytes and tissue macrophages was studied in pig foetuses by immunophenotyping and functional assays. The function of peripheral blood monocytes was compared in germ-free and conventional piglets. First macrophages were identified by electron microscopy in foetal liver on the 25th day of gestation. Monoclonal antibodies against porcine CD45 and SWC3 antigens were used for flow cytometric identification of myelomonocytic cells in cell suspensions prepared from the yolk sac, foetal liver, spleen and cord blood. Leukocytes expressing the common myelomonocytic antigen SWC3 were found in all organs studied since the earliest stages of development. Opsonized zymosan ingestion assay was used to determine the phagocytic capacity of foetal mononuclear phagocytes isolated from cord blood, liver and spleen. In the foetal liver, avid phagocytosis of apoptic cells had been found to occur before cells were able to ingest zymosan in vitro. The first cells capable of ingesting zymosan particles were found on the 40th day of gestation in umbilical blood and 17 days later in foetal spleen and liver. Their relative proportion increased with age. Cord blood monocytes and peripheral blood monocytes in germ-free piglets had low oxidatory burst activity as shown by iodonitrophenyl tetrazolium reduction assay. A remarkable increase of oxidatory burst activity was observed in conventional piglets, probably due to activation of immune mechanisms by the microflora colonizing gastrointestinal tract.  (+info)

Phosphorylation of CD45 by casein kinase 2. Modulation of activity and mutational analysis. (7/2721)

CD45 is a receptor-type protein-tyrosine phosphatase (PTP) that is required for antigen-specific stimulation and proliferation in lymphocytes. This study was designed to determine the nature of specific kinases in lymphocytes that phosphorylate CD45 and to determine the effect of phosphorylation on CD45 PTP activity. A major cytoplasmic lymphocyte kinase that phosphorylated CD45 was identified as casein kinase 2 (CK2) by use of an in-gel kinase assay in combination with immunoprecipitation, immunodepletion, and specific inhibition. Mutational analysis of CK2 consensus sites showed that the target for CK2 was in an acidic insert of 19 amino acids in the D2 domain, and Ser to Ala mutations at amino acids 965, 968, 969, and 973 abrogated CK2 phosphorylation of CD45. CK2 phosphorylation increased CD45 activity 3-fold toward phosphorylated myelin basic protein, and this increase was reversible by PP2A treatment. Mutation of Ser to Glu at the CK2 sites had the same effect as phosphorylation and also tripled the Vmax of CD45. CD45 isolated in vivo was highly phosphorylated and could not be phosphorylated by CK2 without prior dephosphorylation with phosphatase PP2A. We conclude that CK2 is a major lymphocyte kinase that is responsible for in vivo phosphorylation of CD45, and phosphorylation at specific CK2 sites regulates CD45 PTP activity.  (+info)

Differential effects of manipulating signaling in early T cell development in intestinal intraepithelial lymphocytes and thymocytes. (8/2721)

A pre-TCR-CD3 signal is required for the efficient maturation of CD4- CD8- thymocytes to the CD4+ CD8+ stage. This study addressed whether a similar signal is required for maturation of intestinal intraepithelial lymphocytes (IEL) that may develop extrathymically. We have shown previously that IEL from mice deficient for CD3- associated zeta chains include an immature population of CD3- CD8alphaalpha+ cells expressing cytoplasmic TCR beta chains but lacking detectable surface TCRalphabeta, CD16 and B220. Here we stimulated the appearance of such IEL in epsilon+/- zeta-/- mice by expression of an activated Lck transgene or in vivo treatment with anti-CD3epsilon. Anti-CD3epsilon treatment of RAG-deficient animals also yielded CD16- B220- IEL. In contrast, expression of a TCRbeta transgene in rag-1(-/-) mice did not stimulate the appearance of CD3- CD8alphaalpha+ CD16- B220- cells. Taken together these data indicate that although anti-CD3epsilon treatment and LckF505 assist in catalyzing a CD16+ B220+ --> CD16- B220- transition, these manipulations are not equivalent to a pre-TCR signal in IEL lymphocytes.  (+info)

Following M. bovis infection, a marked in vitro expansion suggests that bovine γδ T cells can mount memory-like responses upon restimulation with mycobacterial Ag (Fig. 1) and (reviewed in Ref. 35). However, to date, a combination of surface markers that effectively identify effector/memory-like subsets of bovine Ag-specific γδ T cells have not been reported. In this study, we show that, compared with CD4 T cells, proliferating M. bovis-specific γδ T cells do not significantly alter CD45RO expression after restimulation with mycobacterial Ags in vitro. Our results agree with reports showing that γδ T cells from human TB patients do not modulate CD45RO expression compared with γδ T cells from noninfected subjects (16, 17). Thus, γδ T cells may acquire a preactivated state early in their development and thus, CD45RO is not useful to identify Ag-experienced γδ T cells. In contrast to CD45RO expression, we found that proliferating γδ T cells from M. bovis-infected cattle ...
The effects of a chimeric monoclonal antibody (chA6 mAb) that recognizes both the RO and RB isoforms of the transmembrane protein tyrosine phosphatase CD45 on human T cells were investigated. Chimeric A6 (chA6) mAb potently inhibited antigen-specific and polyclonal T cell responses. ChA6 mAb induced activation-independent apoptosis in CD4 + CD45RO/RB high T cells but not in CD8 + T cells. In addition, CD4 + T cell lines specific for tetanus toxoid (TT) generated in the presence of chA6 mAb were anergic and suppressed the proliferation and interferon (IFN)-γ production by TT-specific effector T cells by an interleukin-10-dependent mechanism, indicating that these cells were equivalent to type 1 regulatory T cells. Similarly, CD8 + T cell lines specific for the influenza A matrix protein-derived peptide (MP.58-66) generated in the presence of chA6 mAb were anergic and suppressed IFN-γ production by MP.58-66-specific effector CD8 + T cells. Furthermore, chA6 mAb significantly prolonged human ...
PTPRC - PTPRC (untagged)-Human protein tyrosine phosphatase, receptor type, C (PTPRC), transcript variant 3 available for purchase from OriGene - Your Gene Company.
Mark, there has been a number of discussions on this list regarding RA/RO... Im not going to rehash those discussions, since I dont want to bore everyone else. Go to the archives and read over the material -- it includes references. But once again, CD45RA+ cells are BOTH naive AND memory. You CANNOT use RA and/or RO to identify naive T cells without an additional marker such as CD62L, CD11a, CD27. As for Double-positive, it depends on how you define positive. Bright (true) RA+RO+ double positives are very rare in peripheral blood but common in active tissues (like tonsil). Cells positive for one and dull for the other are normal resting memory T cells. There are no double-negative cells that are viable. Percentages in the peripheral blood are meaningless for the simple reason that RA+ cells are a heterogeneous mixture of naive & memory. Especially the CD8s, where anywhere between 20 and 80% of RA+ cells can be memory. (In CD4, most (95%) are naive; however, in many disease states ...
Mouse monoclonal antibody raised against native PTPRC. Native purified PTPRC from human thymocytes and T lymphocytes. (MAB4578) - Products - Abnova
CD45 (PTPRC) cloned gene : ORF from ATG to Stop, in pUNO1 expression plasmid selectable in E.coli and mammalian cells. Fully sequenced.
The CD45 molecules are single chain integral membrane proteins comprising at least five isoforms ranging from 180 to 220 kDa. They are generated by alternative splicing combinations of three exons (A, B, and C) of the genomic sequence. The non-restricted CD45, Leucocyte Common Antigen (LCA) consists of an extracellular sequence, proximal to the membrane, which is common to all CD45 isoforms. All the monoclonal antibodies that belong to the CD45 cluster react with this part of the antigen and are able to recognize all CD45 isoforms. These isoforms have extracytoplasmic sequences ranging from 391 to 552 amino acids long with numerous N-linked carbohydrate attachment sites. The cytoplasmic portion contains two phospho-tyrosine-phosphatase domains. The non-restricted CD45 epitope is present on the surface of all human leucocytes, lymphocytes, eosinophils monocytes, basophils and neutrophils by order of decreasing level of expression. CD45 is a major component of the lymphocyte membrane. It is absent ...
The CD45 molecules are single chain integral membrane proteins comprising at least five isoforms ranging from 180 to 220 kDa. They are generated by alternative splicing combinations of three exons (A, B, and C) of the genomic sequence. The non-restricted CD45, Leucocyte Common Antigen (LCA) consists of an extracellular sequence, proximal to the membrane, which is common to all CD45 isoforms. All the monoclonal antibodies that belong to the CD45 cluster react with this part of the antigen and are able to recognize all CD45 isoforms. These isoforms have extracytoplasmic sequences ranging from 391 to 552 amino acids long with numerous N-linked carbohydrate attachment sites. The cytoplasmic portion contains two phospho-tyrosine-phosphatase domains. The non-restricted CD45 epitope is present on the surface of all human leucocytes, lymphocytes, eosinophils monocytes, basophils and neutrophils by order of decreasing level of expression. CD45 is a major component of the lymphocyte membrane. It is absent ...
The CD45 molecules are single chain integral membrane proteins comprising at least five isoforms ranging from 180 to 220 kDa. They are generated by alternative splicing combinations of three exons (A, B, and C) of the genomic sequence. The non-restricted CD45, Leucocyte Common Antigen (LCA) consists of an extracellular sequence, proximal to the membrane, which is common to all CD45 isoforms. All the monoclonal antibodies that belong to the CD45 cluster react with this part of the antigen and are able to recognize all CD45 isoforms. These isoforms have extracytoplasmic sequences ranging from 391 to 552 amino acids long with numerous N-linked carbohydrate attachment sites. The cytoplasmic portion contains two phospho-tyrosine-phosphatase domains. The non-restricted CD45 epitope is present on the surface of all human leucocytes, lymphocytes, eosinophils monocytes, basophils and neutrophils by order of decreasing level of expression. CD45 is a major component of the lymphocyte membrane. It is absent ...
The CD45 antibody recognizes the human CD45 antigen, a tyrosine phosphatase also known as leukocyte common antigen (LCA). The CD45 molecule is required for T and B cell activation and is expressed in at least five isoforms depending on the differentiation status of the cell. The CD45 antibody recognizes a common epitope of all CD45 isoforms. - Australia
|p|CD45 is a 180 - 240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes or platelets. CD45 is a signaling molecule that regulates a va
|p|CD45 is a 180 - 240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes or platelets. CD45 is a signaling molecule that regulates a va
Quantity100 testsVolume1ImmunogenHuman thymocytes and T lymphocytesBackground InformationCD45 (LCA; leukocyte common antigen) is a receptor-type pr...
Quantity100 testsVolume0.4ImmunogenHuman thymocytes and T lymphocytesBackground InformationCD45 (LCA; leukocyte common antigen) is a receptor-type ...
CD45, also known as leukocyte common antigen (LCA) and T200, is expressed on the plasma membrane of all hematopoietic cells except mature red blood cells and platelets.
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Looking for online definition of leukocyte common antigen in the Medical Dictionary? leukocyte common antigen explanation free. What is leukocyte common antigen? Meaning of leukocyte common antigen medical term. What does leukocyte common antigen mean?
Abstract. We recently reported that cross-linking the leukocyte common antigen (CD45) can rapidly induce aggregation of human peripheral blood mononuclear cell
In the present study, we show several new findings on the functional roles of PTP-1B and CD148 in platelet activation. The main finding is that PTP-1B is a selective regulator of SFKs downstream of the integrin αIIbβ3, whereas CD148 is a global regulator of SFKs in resting and activated platelets. As a result, deletion of PTP-1B has much milder effects than deletion of CD148 and additive effects in some instances, such as downstream of αIIbβ3 and CLEC-2, but not GPVI. The other major finding of this study is that PTP-1B is essential for optimal platelet aggregation on collagen under high shear rates, an experience in stenotic arteries. Ablation of PTP-1B results in a dramatic reduction in large aggregate formation but still allows platelets to stick together and form monolayers and smaller aggregates. This is in striking contrast to CD148-deficient platelets, which form very few and small clusters of platelets under the same conditions. These findings have important implications for ...
We examined the developmental profile of TCR-gamma/delta+ cells with respect to CD45RO expression. Although total TCR-gamma/delta+ cells were negligible in the neonatal blood and increased with advancing age, most blood TCR-gamma/delta+ cells markedly expressed CD45RO without a distinction of age, probably reflecting a different CD45RO expression of two subsets defined by BB3 and delta TCS1 mAbs. The vast majority of BB3+ cells expressed CD45RO, whereas expression of CD45RO was virtually absent in the delta TCS1+ population. Functional studies revealed that, while both TCR-gamma/delta+ cell subsets showed CD3-mediated activation, only BB3+ (or Ti gamma A+) cells, but not delta TCS1+ cells, appeared to proliferate in response to PPD in PPD-reactive individuals. The results suggested that the CD45RO+ (BB3+ or Ti gamma A+) subset among blood TCR-gamma/delta+ cells may be mainly involved in the memory or primed component of the immune system responding to some foreign antigens. ...
In this study, we have analyzed the role of IL-10 signaling in CD4 T cells by generating dominant-negative IL-10Rα TG mice. Our results indicated that direct IL-10 signals control colitis development, in particular when caused by the Treg cell-depleted CD45RBlo memory/effector CD4 T cell population. This has also spotlighted characteristic differences in the mechanism of colitis development mediated by CD45RBhi cells or Treg cell-depleted CD45RBlo CD4+ T cells.. It was previously reported by others that activation could reduce the expression of IL-10R1 (IL-10Rα) mRNA in human T cell clones (Liu et al., 1994). We found that although expression of mouse IL-10Rα is reduced upon activation, it recovered after 24-48 h both at the level of mRNA (not depicted) and cell surface protein expression (Fig. S1). In addition, the surface expression of IL-10Rα is detectable at any time of activation by flow cytometry. The IL-10Rα on the surface of T cells is functional after IL-10 stimulation as Stat3 ...
In this study, we have analyzed the role of IL-10 signaling in CD4 T cells by generating dominant-negative IL-10Rα TG mice. Our results indicated that direct IL-10 signals control colitis development, in particular when caused by the Treg cell-depleted CD45RBlo memory/effector CD4 T cell population. This has also spotlighted characteristic differences in the mechanism of colitis development mediated by CD45RBhi cells or Treg cell-depleted CD45RBlo CD4+ T cells.. It was previously reported by others that activation could reduce the expression of IL-10R1 (IL-10Rα) mRNA in human T cell clones (Liu et al., 1994). We found that although expression of mouse IL-10Rα is reduced upon activation, it recovered after 24-48 h both at the level of mRNA (not depicted) and cell surface protein expression (Fig. S1). In addition, the surface expression of IL-10Rα is detectable at any time of activation by flow cytometry. The IL-10Rα on the surface of T cells is functional after IL-10 stimulation as Stat3 ...
Clone REA504 recognizes the rat CD45 antigen, also known as leukocyte common antigen (LCA), which is a 180-220 kDa type I transmembrane protein with multiple isoforms and differential glycosylation. It appears in at least five isoforms depending on the differentiation status of the cell. CD45 is expressed at high levels on all cells of hematopoietic origin except for erythrocyte and platelets. It plays a role in signal transduction through B and T cell antigen receptors. Additional information: Clone REA504 displays negligible binding to Fc receptors. - Suomi
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As you may have noticed, I have not been blogging for a while now and I think am finally starting to miss it. It sort of felt like the purpose why I was so motivated to keep the blog running was slowly fading away. The main goal the last 5 years was to create a memory from my daughters early years mostly for her in the future. The years she would not remember. I wanted to log the travels, the small big moments, my feelings towards her, where we stood for as parents and in life, the baby steps, our values, our life of 3. ...
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In a previous study we isolated a series of rat monoclonal antibodies to the human leukocyte common (LC) antigen and demonstrated that synergistic complement lysis was possible between IgG2b antibodies which recognised different epitopes. In this report we have examined the mechanisms that were involved in synergistic lysis. We found that the number of C1q binding sites increased from 30,000 to 40,000/cell using a single antibody to 90,000/cell when two IgG2b antibodies to different epitopes were used together. The affinity of C1q binding also increased approx. 3-fold for the synergistic pair, and there was a similar increase in the rate of C1 activation. Combinations of an IgG2b with IgG1 or IgG2a gave much smaller increases in the amount of C1q bound and in the rate of C1 activation. Despite the large number of C1q molecules bound with the optimal synergistic pair and the increased rate of C1 activation, lysis was inefficient in serum depleted of Factor D, suggesting a requirement for the alternative
In a previous study we isolated a series of rat monoclonal antibodies to the human leukocyte common (LC) antigen and demonstrated that synergistic complement lysis was possible between IgG2b antibodies which recognised different epitopes. In this report we have examined the mechanisms that were involved in synergistic lysis. We found that the number of C1q binding sites increased from 30,000 to 40,000/cell using a single antibody to 90,000/cell when two IgG2b antibodies to different epitopes were used together. The affinity of C1q binding also increased approx. 3-fold for the synergistic pair, and there was a similar increase in the rate of C1 activation. Combinations of an IgG2b with IgG1 or IgG2a gave much smaller increases in the amount of C1q bound and in the rate of C1 activation. Despite the large number of C1q molecules bound with the optimal synergistic pair and the increased rate of C1 activation, lysis was inefficient in serum depleted of Factor D, suggesting a requirement for the alternative
Protein tyrosine phosphatase CD45 is a key player in T-cell receptor signaling and lymphocyte development. Differential expression of multiple CD45 isoforms resulting from the alternative splicing of exons A, B, and C, which encode part of the extracellular domain, is an important feature of CD45 expression. We report a novel isoform that results from the alternative splicing of a previously undiscovered exon between the constitutively spliced exon 3 and the alternatively spliced exon A. This 123-bpâ€:#x0093:long exon encodes 41 amino acids and is unlikely to undergo te extensive glycosylation seen for the regions encoded by exons A, B, and C. Reverse transcriptase polymerase chain reaction demonstrates that this isoform is expressed in human peripheral blood mononuclear cells and cell lines of lymphoid origin, but with a clearly different pattern to that of the isoforms caused by exons A, B, and C, implying a different regulatory mechanism.
Abstract. Abstract 3988Multiple myeloma (MM) is an incurable plasma cell malignancy predominantly negative for the surface protein tyrosine phosphatase CD45. P
The RA3.3A1/6.1 monoclonal antibody reacts with mouse B220 also known as CD45R. B220 is a 220 kDa transmembrane protein tyrosine phosphatase expressed on B cells and some subsets of T and NK cells. B220 plays a critical role in TCR and BCR signaling and is commonly used as a B cell marker. The RA3.3A1/6.1 antibody is commonly used for |em|in vivo|/em| B cell depletion.
The lungs, immune organs, and systemic small vessels are the main targets of virus attack, so that extensive consolidation of the lung, diffuse alveolar damage with hyaline membrane formation, respiratory distress, and decreased immune function are the main causes of death. (A) H&E (original magnification ×400). The marginal sinus and germinal centres disappeared in some lymph nodes, and many monocytes and plasmacytoid monocytes could be seen in the remaining lymphatic sinus. Available under CC-BY-SA license. Immunoinformatics‐aided identification of T cell and B cell epitopes in the surface glycoprotein of 2019‐nCoV. Viral particles were identified by transmission electron microscopy. Systemic toxic changes included degeneration and necrosis of the parenchyma cells in the lung, liver, kidney, heart, and adrenal gland. 10.1016/j.jstrokecerebrovasdis.2020.105182. Cytokeratin (CK, 1 : 50), epithelial membrane antigen (EMA, 1 : 100), CD68 (1 : 100), and leukocyte common antigen (LCA, 1 : 100) ...
Sigma-Aldrich offers abstracts and full-text articles by [Valentina Salzman, Valentina Porro, Mariela Bollati-Fogolín, Pablo S Aguilar].
Use our protocol compatibility table to understand which immune signaling and phenotyping antibodies will work together in your multicolor flow cytometry experiment.
Hello everyone, Weve talked before about where and how B cells mature from naive cells to memory cells, and how that process might be responsible for autoimmunity. During an infection, B cells migrate to the so-called Germinal centres where they ...
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Попробуйте оправдать игровые автоматы Вокруг киберпространства, которое вы покидаете, вы услышите, как веб-сайты дают вам возможность сыграть в онлайн-слоты на мертвую версию. Если вы выполнили загрузку «Кассино», то вы точно поймете, что нужно выиграть в следующих четырех казино. В эдикте, чтобы исключить очередное разочарование в слотах, вы можете захотеть получить авто, которое завещает гарантированную плату, сопоставимую с тем, что на самом деле говорится. Осуществление выставки дает вам возможность убедиться, что мерчендайзинг был просто ...
Попробуйте оправдать игровые автоматы Вокруг киберпространства, которое вы покидаете, вы услышите, как веб-сайты дают вам возможность сыграть в онлайн-слоты на мертвую версию. Если вы выполнили загрузку «Кассино», то вы точно поймете, что нужно выиграть в следующих четырех казино. В эдикте, чтобы исключить очередное разочарование в слотах, вы можете захотеть получить авто, которое завещает гарантированную плату, сопоставимую с тем, что на самом деле говорится. Осуществление выставки дает вам возможность убедиться, что мерчендайзинг был просто ...
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Before I get this party started, I must say thanks to everyone for all your sweet prayers, thoughts and phone calls. Its always a blessing to feel loved! In case you dont know or havent heard yet, Lovebug was involved in a car chase /shooting / fight while working last Tuesday night. During a physical altercation with one of the…
Complete information for PTPRC gene (Protein Coding), Protein Tyrosine Phosphatase Receptor Type C, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
CD45RA is a specific splice variant of the transmembrane tyrosine phosphatase CD45. The CD45RA isoform is most highly expressed on resting/naive T cells, B cells and monocytes.
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WATER FOR THE ORIGINATION OF LIFE. Which water is optimal for the origin (generation) of life? The memory of water is an informational medium for the biggest miracle in nature. More information:. http://www.medicalbiophysics.dir.bg/de/water_memory.html. Memory of Water an Origin of Living Matter Bioresonance Effects. Dr. Ignat Ignatov - Biophysicist. 2006 Sofia, Bulgarien. ...
He was completely out of the water now, bare feet against the untamed earth. The grass was soft, but jagged pine needles lay in wait beneath it. Antonius felt none of it, his feet numbed to the sensations. He stared at Abigail, his height towering over her, looking considerably intimidating without trying. He was waiting for her reply and instead, saw that she had once again remembered his state of undress.. The obvious was no concern of hers, her eyes too caught on his chest and the jagged scars that marked it. Two were long and particularly nasty looking, one moving over his pectoral and the other looking as if it had been a harsh stab near his kidney. The others were small and faded, but those two were like glaring beacons in the night, somewhat new, but closed and freshly scarred. A sudden, shuddering thought came to her mind-what did he do when he was a werewolf? She had never truly considered it before and now felt immensely foolish.. She had moved closer to him and not realized it until ...
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On Wed, Apr 30, 2008 at 10:09:04AM +0100, Richard Earnshaw wrote: , The compiler never directly generates SWP, so this must be an in-line , assembler statement. Since theres no SWP instruction in Thumb, the , code that generates this will need reworking to push the required , instructions out-of-line. Ok, I tried finding the culprit asm code but failed to notice sys/arch/arm/include/lock.h: ... #if defined(_KERNEL) static __inline int __swp(int __val, volatile unsigned char *__ptr) { __asm volatile(swpb %0, %1, [%2] : =&r (__val) : r (__val), r (__ptr) : memory); return __val; } #else static __inline int __swp(int __val, volatile int *__ptr) { __asm volatile(swp %0, %1, [%2] : =&r (__val) : r (__val), r (__ptr) : memory); return __val; } #endif /* _KERNEL */ ... static __inline void __attribute__((__unused__)) __cpu_simple_lock(__cpu_simple_lock_t *alp) { while (__swp(__SIMPLELOCK_LOCKED, alp) != __SIMPLELOCK_UNLOCKED) continue; } static __inline int ...
FMO controls are samples that contain all the antibodies you are testing in your experimental samples, minus one of them. When analyzing the minus, or left out parameter in an FMO control, you give yourself a strong negative control to work with. Its a strong negative control because the left out marker in the FMO control allows you to take into account how the other stains in your panel affect the respective minus parameter. Many flow cytometry gates are difficult to define. This is especially true when youre looking at activation markers within a continuum or accounting for the large data spread that occurs when compensating a 10+ color experiment. The only way to convince reviewers that your gate is in the proper place is by using FMO controls. Heres why you need to use FMO controls for any multicolor flow cytometry experiment and how to prepare these controls properly.. Read More ...
Single-nucleotide polymorphisms in NAGNAG acceptors are highly predictive for variations of alternative splicing. [PMID 18634151] No association between systemic sclerosis and C77G polymorphism in the human PTPRC (CD45) gene. [PMID 20158892 ...
The revelation of the memories of previous lives, on the other hand-that is, the revelation of what has been experienced by selves and thinkers prior to the present self and thinker-consists of a time-reversal to a time either hundreds or thousands of years before the present time (parallel to the time-reversal from 55 to 44 in the above report), which is then followed by a progression of time in a forward direction (parallel to the forward movement of time from 44 to 55-and, 33 minutes later, to 28-in the above report) through those experiences in precisely the same way they were first experienced; that is, without any knowledge of either future time or future experiences of other selves and thinkers. In other words, a memory of a previous life does not perfectly fit the definition of a memory as experienced, or defined, by a self or a thinker. That is, one does not really remember what those previous selves and thinkers have experienced. Rather, it is the re-experience ...
Khan bantered saying soon after the injection was pushed into his body, he thought all his problems magically vanished, and so did his memory.
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Pao LI, Bedzyk WD, Persin C, Cambier JC (February 1997). "Molecular targets of CD45 in B cell antigen receptor signal ... recruitment to B cell antigen receptor and activation following stimulation of CD45- B cells". Journal of Immunology. 158 (6): ... Vilen BJ, Famiglietti SJ, Carbone AM, Kay BK, Cambier JC (July 1997). "B cell antigen receptor desensitization: disruption of ... Tissue Antigens. 69 (2): 145-53. doi:10.1111/j.1399-0039.2006.00710.x. PMID 17257317. BTLA+protein,+human at the US National ...
2000). "CD45-associated protein is not essential for the regulation of antigen receptor-mediated signal transduction". Eur. J. ... 2004). "PTPRC (CD45) C77G mutation does not contribute to multiple sclerosis susceptibility in Sardinian patients". J. Neurol. ... Vogel A, Strassburg CP, Manns MP (2003). "77 C/G mutation in the tyrosine phosphatase CD45 gene and autoimmune hepatitis: ... 1994). "LPAP, a novel 32-kDa phosphoprotein that interacts with CD45 in human lymphocytes". J. Biol. Chem. 269 (46): 29102-11. ...
In these cells, a small amount of LYN is associated with cell surface receptor proteins, including the B cell antigen receptor ... Greer SF, Justement LB (May 1999). "CD45 regulates tyrosine phosphorylation of CD22 and its association with the protein ... Brown VK, Ogle EW, Burkhardt AL, Rowley RB, Bolen JB, Justement LB (Jun 1994). "Multiple components of the B cell antigen ... Yamamoto T, Yamanashi Y, Toyoshima K (Apr 1993). "Association of Src-family kinase Lyn with B-cell antigen receptor". ...
Marker analysis has also led to debate over the origin of the cell type; it coexpresses CD45, a leukocyte common antigen, and ... centers of lymphoid follicles and have an integral role in regulation of the germinal center reaction and present antigens to B ... CD15, a monocyte common antigen. Because of the debate and difficulty of staining, pathologic diagnosis often requires ...
1994). "Multiple components of the B cell antigen receptor complex associate with the protein tyrosine phosphatase, CD45". J. ... It is associated with agammaglobulinemia-6. The B lymphocyte antigen receptor is a multimeric complex that includes the antigen ... PDBe-KB provides an overview of all the structure information available in the PDB for Human B-cell antigen receptor complex- ... Müller B, Cooper L, Terhorst C (1995). "Interplay between the human TCR/CD3 epsilon and the B-cell antigen receptor associated ...
They express LCAs (leucocyte common antigens) CD45, CD14, CD33, and CD4 (also expressed by T helper cells). These histiocytes ... Their main activity is antigen presentation; they express Factor XIIIa, CD1c, and Class II Human leukocyte antigens. A subset ... Langerhans cells are antigen-presenting cells but have undergone further differentiation. Skin Langerhans cells express CD1a, ... Phagocytosis is the main process of macrophages and antigen presentation the main property of dendritic cells (so called ...
Reth M (1992). "Antigen receptors on B lymphocytes". Annual Review of Immunology. 10 (1): 97-121. doi:10.1146/annurev.iy. ... CD45". The Journal of Biological Chemistry. 269 (25): 17238-44. PMID 7516335. Pani G, Kozlowski M, Cambier JC, Mills GB, ... Engels N, Wollscheid B, Wienands J (Jul 2001). "Association of SLP-65/BLNK with the B cell antigen receptor through a non-ITAM ... Brown VK, Ogle EW, Burkhardt AL, Rowley RB, Bolen JB, Justement LB (Jun 1994). "Multiple components of the B cell antigen ...
... is also known as CD45 antigen (CD stands for cluster of differentiation), which was originally called leukocyte common ... A unique secreted adenovirus E3 protein binds to the leukocyte common antigen CD45 and modulates leukocyte functions. Proc Natl ... CD45 does not colocalize with lipid rafts on murine and human non-transformed hematopoietic cells, but CD45 positioning within ... May 2004). "CD45 variant alleles: possibly increased frequency of a novel exon 4 CD45 polymorphism in HIV seropositive Ugandans ...
If the KIR receptors of NK cell find their cognate antigen on the surface of target cell, formation of the lytic synapse is ... d-SMAC (distal-SMAC) enriched in CD43 and CD45 molecules. New investigations, however, have shown that a "bull's eye" is not ... The process of formation begins when the T-cell receptor (TCR) binds to the peptide:MHC complex on the antigen-presenting cell ... Play media In immunology, an immunological synapse (or immune synapse) is the interface between an antigen-presenting cell or ...
Cd45, a cell surface glycoprotein, plays a critical role in antigen-stimulated dephosphorylation of specific phosphotyrosines ... Hermiston ML, Zikherman J, Zhu JW (March 2009). "CD45, CD148, and Lyp/Pep: critical phosphatases regulating Src family kinase ...
"Physical associations between CD45 and CD4 or CD8 occur as late activation events in antigen receptor-stimulated human T cells ... The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen- ... The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell ... Barber EK, Dasgupta JD, Schlossman SF, Trevillyan JM, Rudd CE (1989). "The CD4 and CD8 antigens are coupled to a protein- ...
They also lack the expression of markers CD14, CD34, CD45, which can be important in the ability of stromal cells to remain ... Low levels of human leukocyte antigen (HLA-DR) make MSC's hypoimmunogenic. MSC's have trilineage differentiation where they are ...
During ligand binding, CD45 and CD148 are excluded from the TCR region. It was also shown that both the truncation of CD45 and ... The TCR/peptide-MHC complex, formed when a T cell recognises its ligand on an antigen presenting cell (APC) and the T-cell-APC ... CD45 phosphatase is not completely excluded from the close-contact zone and thus the signal generated in the case of a ... Phosphatases CD45 and CD148 with much larger ectodomains than TCR are sterically excluded from the close contact zones, while ...
CD4 antigen - CD45 antigen - CD95 antigen - CDC28 protein kinase - cell - cell adhesion molecule - Cell biology - cell cycle ... T-cell antigen receptors - tachykinin - tachykinin receptor - talin protein - tandem repeat sequence - taste bud - TATA box - ... carcinoembryonic antigen - carrier - carrier protein - CAS registry number - casein - catabolism - catalyst - catalytic domain ... alpha-beta T-cell antigen receptor - alpha-fetoprotein - alpha-globulin - alpha-macroglobulin - alpha-MSH - Ames test - amide ...
CD45 antigens, and many other surface receptors required to act in the antigen-presenting, phagocytic, and cytotoxic roles that ... In some cases, microglia can also be activated by IFN-γ to present antigens, but do not function as effectively as if they had ... As mentioned above, resident non-activated microglia act as poor antigen presenting cells due to their lack of MHC class I/II ... Once they have been presented with antigens, T-cells go on to fulfill a variety of roles including pro-inflammatory recruitment ...
... antigens, cd45 MeSH D08.811.277.352.650.625.700.200 - cdc25 phosphatase MeSH D08.811.277.352.650.625.725 - pyruvate ... antigens, cd38 MeSH D08.811.277.450.770 - oligo-1,6-glucosidase MeSH D08.811.277.450.770.800 - sucrase-isomaltase complex MeSH ... prostate-specific antigen MeSH D08.811.277.656.300.760.442.875 - tissue kallikreins MeSH D08.811.277.656.300.760.501 - mannose- ... antigens, cd13 MeSH D08.811.277.656.350.555.200 - carboxypeptidase b MeSH D08.811.277.656.350.555.250 - carboxypeptidase h MeSH ...
... and CD79a and also express the common leukocyte antigen CD45, which is uncommon on RSH cells. The LP cells have scant cytoplasm ...
... antigens, cd40 MeSH D23.050.301.264.035.143 - antigens, cd43 MeSH D23.050.301.264.035.145 - antigens, cd45 MeSH D23.050.301.264 ... antigens, cd40 MeSH D23.101.100.110.143 - antigens, cd43 MeSH D23.101.100.110.145 - antigens, cd45 MeSH D23.101.100.110.146 - ... antigens, cd15 MeSH D23.101.100.900.131 - antigens, cd31 MeSH D23.101.100.920 - antigens, ly MeSH D23.101.100.930 - antigens, ... forssman antigen MeSH D23.050.285.018 - antigens, cd24 MeSH D23.050.285.025 - antigens, cd30 MeSH D23.050.285.040 - antigens, ...
For example, when an antigen-presenting cell displays a peptide antigen on MHC class II proteins, a CD4+ cell will aid those ... Lck activation is controlled by the opposing actions of CD45 and Csk. CD45 activates Lck by dephosphorylating a tyrosine in its ... During an immune response, professional antigen-presenting cells (APCs) endocytose antigens (typically bacteria or viruses), ... that a host antigen is foreign. As a result, the CD8+ T cells treat the host cell presenting that antigen as infected, and go ...
Evidence for this model is given by the observation that in T cells, phosphatases CD45 and CD148 segregate from the T-cell ... Li HL, Davis W, Puré E (April 1999). "Suboptimal cross-linking of antigen receptor induces Syk-dependent activation of p70S6 ... Irles C, Symons A, Michel F, Bakker TR, van der Merwe PA, Acuto O (February 2003). "CD45 ectodomain controls interaction with ... May 2016). "Initiation of T cell signaling by CD45 segregation at 'close contacts'". Nature Immunology. 17 (5): 574-582. doi: ...
It is only when the cell comes in contact with an antigen presenting cell that the larger CD45 is displaced due to the close ... Antigens that activate B cells with the help of T-cell are known as T cell-dependent (TD) antigens and include foreign proteins ... Antigens that activate B cells without T cell help are known as T cell-independent (TI) antigens and include foreign ... As with TD antigens, B cells activated by TI antigens need additional signals to complete activation, but instead of receiving ...
"Striking similarities between antigen receptor J pieces and sequence in the second chain of the murine CD8 antigen". Nature. ... Johnson helped to establish the function of CD45 as a critical protein tyrosine phosphatase in T cell activation and defined ... "CD45 regulates GM-CSF, retinoic acid and T cell homing in intestinal inflammation". Mucosal Immunology. 9 (6): 1514-1527. doi: ... "Mutational analysis of CD45, a leukocyte specific tyrosine phosphatase". Journal of Biological Chemistry. 267 (12): 8035-8041. ...
... small clusters of microglial cells with enhanced major histocompatibility complex class II antigen, CD45 and CD68 antigen ... December 2008). "Antigen microarrays identify unique serum autoantibody signatures in clinical and pathologic subtypes of ... "EBNA1-specific T cells from patients with multiple sclerosis cross react with myelin antigens and co-produce IFN-gamma and IL-2 ...
With the assistance of a phosphatase present on the intracellular section of CD45 (common leukocyte antigen), these molecules ... For example, when an antigen-presenting cell expresses an antigen on MHC class II, a CD4+ cell will aid those cells through a ... It is unknown what role the relatively bulky extracellular region of CD45 plays during cell interactions, but CD45 has various ... that a host antigen is foreign. As a result, the CD8+ T cells treat the host cell presenting that antigen as infected, and go ...
This process prevents the circulation of T cells that are self-reactive and recognise self antigen. Both galectin-1 and ... CD43 and CD45 are involved in apoptosis. Galectin-7 is expressed under the p53 promoter and may have a key role in regulating ...
... +Antigen at the US National Library of Medicine Medical Subject Headings (MeSH) Mouse CD Antigen Chart Human CD Antigen ... Regulation by the CD45 tyrosine phosphatase". J. Immunol. 145 (8): 2448-54. PMID 1976695. Luzzati AL, Pugliese O, Giacomini E, ... The great majority of T cell lymphomas and leukaemias also express CD2, making it possible to use the presence of the antigen ... It has also been called T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, erythrocyte receptor and rosette receptor. It ...
Regulation by the CD45 tyrosine phosphatase.". J. Immunol. 145 (8): 2448-54. PMID 1976695.. ... 1992). "The antigen-specific induction of normal human lymphocytes in vitro is down-regulated by a conserved HIV p24 epitope ... Schraven B, Samstag Y, Altevogt P, Meuer SC (1990). "Association of CD2 and CD45 on human T lymphocytes.". Nature 345 (6270): ... 1986). "The sheep erythrocyte receptor and both alpha and beta chains of the human T-lymphocyte antigen receptor bind the ...
This antigen along with other blood group antigens was used to identify the Basque people as a genetically separate group.[49] ... Because the Duffy antigen is uncommon in those of Black African descent, the presence of this antigen has been used to detect ... The Fy4 antigen, originally described on Fy (a-b-) RBCs, is now thought to be a distinct, unrelated antigen and is no longer ... The Duffy antigen is expressed in greater quantities on reticulocytes than on mature erythrocytes.[21] While the Duffy antigen ...
Her PhD supervisor was Eric Bell , the first to describe CD45 expression on memory T cells. After her PhD, she completed ... Marshall's work has investigated how mast cells are involved in the early immune response to infection and antigen. She is best ... One of which Depends on Persisting Antigen". The Journal of Experimental Medicine. 185 (4): 767-776. doi:10.1084/jem.185.4.767 ... roles of mast cells and their ability to mobilize dendritic cells during the early immune response to infection or antigen. Her ...
Sialyl Lewis X is also one of the most important blood group antigens and is displayed on the terminus of glycolipids that are ... Immunohistochemical panels for the diagnosis of Hodgkins disease typically employ CD15 along with CD30 and CD45; the latter ... Sialyl Lewis x is also an inflammation-associated antigen on liver cells. It becomes over expressed on diseased liver cells and ... Sialyl LewisX (sLeX) also known as cluster of differentiation 15s (CD15s) or stage-specific embryonic antigen 1 (SSEA-1), is a ...
Surface antigens[edit]. Terminally differentiated plasma cells express relatively few surface antigens, and do not express ... the Interleukin-6 receptor and lack of expression of CD45. In humans, CD27 is a good marker for plasma cells, naive B cells are ... Another important surface antigen is CD319 (SLAMF7). This antigen is expressed at high levels on normal human plasma cells. It ... After leaving the bone marrow, the B cell acts as an antigen presenting cell (APC) and internalizes offending antigens, which ...
2000). "Characterization of a new member of the TNF family expressed on antigen presenting cells.". Biol. Chem. 380 (12): 1443- ... CD45 • CD46 • CD47 • CD48 • CD49 (a, b, c, d, e, f) • CD50 ... "BLyS receptor signatures resolve homeostatically independent compartments among naïve and antigen-experienced B cells.". Semin ...
This is carried out by using donor-derived antigen-presenting cells. These new methods have reduced culture time to 10-12 days ... CD45 deficiency, CD3δ/CD3ε deficiency. T-/B- SCID (both T and B cells absent): RAG 1/2 deficiency, DCLRE1C deficiency, ... recurrent infections and failure of the development of antibodies on exposure to antigens. The 1999 criteria also distinguish ... selective immunoglobulin A deficiency Specific antibody deficiency to specific antigens with normal B cell and normal Ig ...
I. Partial characterization of soluble Ki-1 antigen and detection of the antigen in cell culture supernatants and in serum by ... Josimovic-Alasevic O, Dürkop H, Schwarting R, Backé E, Stein H, Diamantstein T (Jan 1989). "Ki-1 (CD30) antigen is released by ... CD30+Antigens at the US National Library of Medicine Medical Subject Headings (MeSH) ... results from cDNA cloning and sequence comparison of the CD30 antigen from different sources". Molecular Immunology. 31 (17): ...
T-cell sensitivity to antigen could be increased via avidity-based mechanism. The antigen sensitivity is higher in antigen- ... CD45 - a transmembrane protein whose intracellular tail functions as a tyrosine phosphatase that activates Src family kinases ... Each recombined TCR possess unique antigen specificity, determined by the structure of the antigen-binding site formed by the α ... many TCRs recognize the same antigen peptide and many antigen peptides are recognized by the same TCR.[2] ...
Macrophage-1 antigen (CD11b+CD18). *VLA-4 (CD49d+CD29). *Glycoprotein IIb/IIIa (ITGA2B+ITGB3) ...
Seligman P. A., Butler C. D., Massey E. J., etal. The p97 antigen is mapped to the q24-qter region of chromosome 3; the same ... Le Beau M. M., Diaz M. O., Plowman G. D., etal. Chromosomal sublocalization of the human p97 melanoma antigen. (англ.) // Hum. ... Plowman G. D., Brown J. P., Enns C. A., etal. Assignment of the gene for human melanoma-associated antigen p97 to chromosome 3 ... Rose T. M., Plowman G. D., Teplow D. B., etal. Primary structure of the human melanoma-associated antigen p97 ( ...
Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) also known as CD66e (Cluster of Differentiation 66e), is a ... 2001). "Heterogeneous RNA-binding protein M4 is a receptor for carcinoembryonic antigen in Kupffer cells". J. Biol. Chem. 276 ( ... CEACAM5, CD66e, CEA, carcinoembryonic antigen related cell adhesion molecule 5. External IDs. HomoloGene: 128801 GeneCards: ... Oikawa S, Nakazato H, Kosaki G (1987). "Primary structure of human carcinoembryonic antigen (CEA) deduced from cDNA sequence". ...
The protein also carries the Jr(a) antigen, which defines the Junior blood group system.[9] ...
van Rhenen A., van Dongen G. A., Kelder A., et al. The novel AML stem cell associated antigen CLL-1 aids in discrimination ...
"Interaction of glycogen synthase kinase 3beta with the DF3/MUC1 carcinoma-associated antigen and beta-catenin". Molecular and ...
Ebert LM, McColl SR (2002). "Up-regulation of CCR5 and CCR6 on distinct subpopulations of antigen-activated CD4+ T lymphocytes ... This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may ... dendritic cells induce antitumor immunity when genetically fused with nonimmunogenic tumor antigens". J. Immunol. 167 (11): ...
Rozbicie tych kompleksów powoduje utratę funkcji CD4[5]. Funkcja[edytuj , edytuj kod]. CD4 jest jednym z koreceptorów receptora ... The CD4 and CD8 T cell surface antigens are associated with the internal membrane tyrosine-protein kinase p56lck.. „Cell". 55 ( ... Constitutively active Lck kinase in T cells drives antigen receptor signal transduction.. „Immunity". 32 (6), s. 766-77, Jun ... Nomenclature for clusters of differentiation (CD) of antigens defined on human leukocyte populations. IUIS-WHO Nomenclature ...
W ten sposób zidentyfikowano limfocyty Ts jako populację o niskiej ekspresji białek CD5[16], CD45RB[17] i CD45RC[18]. Problemem ... Antigen-specific peripheral shaping of the natural regulatory T cell population. „J Exp Med". 205 (13), s. 3105-3117, grudzień ... De novo production of antigen-specific suppressor cells in vivo. „Nat Protoc". 1 (2), s. 653-661, 2006. PMID: 17802642. ... Regulation of the immune response to tumor antigens. X. Activation of third-order suppressor T cells that abrogate anti-tumor ...
It is also called Lewis x and SSEA-1 (stage-specific embryonic antigen 1) and represents a marker for murine pluripotent stem ... Immunohistochemical panels for the diagnosis of Hodgkins disease typically employ CD15 along with CD30 and CD45; the latter ... CD15 Antigen at the US National Library of Medicine Medical Subject Headings (MeSH) ... CD15 (3-fucosyl-N-acetyl-lactosamine) is a cluster of differentiation antigen - an immunologically significant molecule. CD15 ...
A new ligand for human leukocyte antigen class II antigens". The Journal of Experimental Medicine. 176 (2): 327-37. doi:10.1084 ... A new ligand for human leukocyte antigen class II antigens". The Journal of Experimental Medicine. 176 (2): 327-37. doi:10.1084 ... antigen processing and presentation of exogenous peptide antigen via MHC class II. ... antigen binding. • transmembrane signaling receptor activity. • MHC class II protein binding. Cellular component. • membrane. • ...
... antigen je protein koji je kod ljudi kodiran CD97 genom.[1][2][3] ... CD45 • CD46 • CD47 • CD48 • CD49 (a, b, c, d, e, f) • CD50 ... 2001). "Tissue distribution of the human CD97 EGF-TM7 receptor". Tissue Antigens 57 (4): 325-31. PMID 11380941. doi:10.1034/j. ... "Expression cloning and chromosomal mapping of the leukocyte activation antigen CD97, a new seven-span transmembrane molecule of ...
"Entrez Gene: ITGB3 integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)".. *^ May, K. E.; Villar, J.; Kirtley, S.; ... CD61+Antigens at the US National Library of Medicine Medical Subject Headings (MeSH) ...
The Reed-Sternberg cells are identified as large often bi-nucleated cells with prominent nucleoli and an unusual CD45-, CD30+, ... is not routinely used to treat Hodgkin lymphoma due to the lack of CD20 surface antigens in most cases. The use of rituximab in ...
"Direct association of adenosine deaminase with a T cell activation antigen, CD26". Science. 261 (5120): 466-9. doi:10.1126/ ...
B cells can present antigens to a specialized group of helper T cells called TFH cells. If an activated TFH cell recognizes the ... Roles of T cell-B-cell-activating molecule (5c8 antigen) and CD40 in contact-dependent help". Journal of Immunology. 149 (12): ... It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In ... Grewal, IS; Xu, J; Flavell, RA (7 December 1995). "Impairment of antigen-specific T-cell priming in mice lacking CD40 ligand". ...
antigen processing and presentation of peptide antigen via MHC class I. • antigen processing and presentation of exogenous ... antigen processing and presentation of exogenous peptide antigen via MHC class I. • lipoprotein transport. • negative ... peptide antigen via MHC class I, TAP-dependent. • platelet degranulation. • MyD88-dependent toll-like receptor signaling ...
Primarily, the VCAM-1 protein is an endothelial ligand for VLA-4 (Very Late Antigen-4 or integrin α4β1) of the β1 subfamily of ...
... uveitis antigens induce CXCR3- and CXCR5-expressing lymphocytes and immature dendritic cells to migrate (англ.) // Blood (англ ...
In addition to aiding with cytotoxic T cell antigen interactions the CD8 co-receptor also plays a role in T cell signaling. The ... the CD8 co-receptor plays a role in T cell signaling and aiding with cytotoxic T cell antigen interactions. ... This affinity keeps the T cell receptor of the cytotoxic T cell and the target cell bound closely together during antigen- ... Once the T cell receptor binds its specific antigen Lck phosphorylates the cytoplasmic CD3 and ζ-chains of the TCR complex ...
antigen binding. • virus receptor activity. • protein binding. • transmembrane signaling receptor activity. • identical protein ...
1996). "CD88 antibodies specifically bind to C5aR on dermal CD117+ and CD14+ cells and react with a desmosomal antigen in human ... CD45 • CD46 • CD47 • CD48 • CD49 (a, b, c, d, e, f) • CD50 ...
CD74 (англ. HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain) - мембранный белок ... II histocompatibility antigen gamma chaingamma chain of class II antigensIiHLA-DR antigens-associated invariant chainIa antigen ... Riberdy J.M., Newcomb J.R., Surman M.J., Barbosa J.A., Cresswell P. HLA-DR molecules from an antigen-processing mutant cell ... Machamer C.E., Cresswell P. Biosynthesis and glycosylation of the invariant chain associated with HLA-DR antigens (англ.) // ...
CD45 antigen explanation free. What is CD45 antigen? Meaning of CD45 antigen medical term. What does CD45 antigen mean? ... Looking for online definition of CD45 antigen in the Medical Dictionary? ... CD45 antigen , definition of CD45 antigen by Medical dictionary https://medical-dictionary.thefreedictionary.com/CD45+antigen ... CD45. (redirected from CD45 antigen) CD45. a type I transmembrane protein present on all hemopoietic cells (except erythrocytes ...
The CD45 antigens occur as multiple isoforms that result from alternative mRNA splicing and differential usage of three exons. ... CD45 Antigens: High-molecular weight glycoproteins uniquely expressed on the surface of LEUKOCYTES and their hemopoietic ... CD45 Antigen; Antigens, CD45; 2H4 Antigens; B220 Antigen; B220 Antigens; CD45R0 Antigens; CD45RB Antigens; CD45RCAntigens; L-CA ... Leukocyte Common Antigen; T200 Antigens; Antigen, B220; Antigen, CD45; Antigen, Leukocyte Common; Antigens, 2H4; Antigens, B220 ...
The CD45 tyrosine phosphatase regulates specific pools of antigen receptor-associated p59fyn and CD4-associated p56lck tyrosine ... which did not express the CD45 phosphotyrosine phosphatase. The cells were sorted into CD45- and CD45+ populations and shown to ... Activation by CD45 of these receptor-associated kinase pools correlates with the ability of the TCR and its coreceptors to ... Using this technique it was shown that cell surface CD4-p56lck kinase activity was 78% lower in CD45- than in CD45+ cells. ...
The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed ...
Induction of CD45 isoform switch in murine B cells by antigen receptor stimulation and by phorbol myristate acetate and ...
Human Protein Tyrosine Phosphatase CD45 (PTPRC) Antigen Immunoaffinity Purified Polyclonal \ X2178P for more molecular products ... Antigen Immunoaffinity Purified Polyclonal. Related products : Human Protein Tyrosine Phosphatase CD45 (PTPRC) Antigen ... Antigen Immunoaffinity Purified Polyclonal / Product Detail : X2178P Human Protein Tyrosine Phosphatase CD45 (PTPRC) Antigen ... Human Protein Tyrosine Phosphatase CD45 (PTPRC) Antigen Immunoaffinity Purified Polyclonal antibody storage GENTAUR recommends ...
CD45" by people in this website by year, and whether "Antigens, CD45" was a major or minor topic of these publications. ... The CD45 antigens occur as multiple isoforms that result from alternative mRNA splicing and differential usage of three exons. ... "Antigens, CD45" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... Below are the most recent publications written about "Antigens, CD45" by people in Profiles. ...
Washington University Physicians. Department of Pathology & Immunology. 509 S. Euclid Ave.. St. Louis, MO 63110. Phone: 314-747-1100 , Fax: 314-362-4080. [email protected] Contact us ». ...
Leukocyte Common Antigens. LinkOut - more resources. Full Text Sources. *Elsevier Science. Medical. *Immune System and ... Previously, the serpentine twists and turns of the CD45 research field have tended to draw attention to CD45 either as a ... This review draws heavily on CD45 knockout mouse data to emphasize that CD45 has both positive and negative actions in ... The CD45 tyrosine phosphatase: a positive and negative regulator of immune cell function.. Alexander DR1. ...
On human leucocytes, five different isoforms of CD45 have been identified, named ABC, AB, BC, B and 0. Clone 2B11 reacts with ... all known isotypes of the CD45 family and clone PD7/26 has been clustered as anti-CD45RB. The antibody is a useful aid for ... CD45 is a transmembrane glycoprotein expressed on most nucleated cells of hematopoetic origin. ... FLEX Monoclonal Mouse Anti-Human CD45, Leucocyte Common Antigen, Clones 2B11 + PD7/26, Ready-to-Use (Dako Omnis) - EN / FR / DE ...
Subsets of rat CD4+ T cells defined by their differential expression of variants of the CD45 antigen: developmental ... Subsets of rat CD4+ T cells defined by their differential expression of variants of the CD45 antigen: developmental ... Subsets of rat CD4+ T cells defined by their differential expression of variants of the CD45 antigen: developmental ... Animals, Antigens, Differentiation, B-Lymphocytes, CD4-Positive T-Lymphocytes, Histocompatibility Antigens, Interleukin-2, ...
CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, ce ... CD45 is known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all ... CD45 is known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all ... CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3 ...
CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the ... Antigen References 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.. 2. Trowbridge IS, et al. 1993. ... Antigen Details Structure Protein tyrosine phosphatase (PTP) family, 180-240 kD Distribution All hematopoietic cells except ... CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation ...
Leukocyte Common Antigens / immunology* * Leukocytes / cytology * Leukocytes / immunology* * Lymphatic Vessels / cytology * ... We observed a striking co-localization of CD45(+) leukocytes with the developing lymphatics. Numerous CD45(+) cells expressed ... Integration of CD45-positive leukocytes into newly forming lymphatics of adult mice Histochem Cell Biol. 2016 Jun;145(6):629-36 ... This indicates that, similar to inflammation-induced lymphangiogenesis in man, circulating CD45(+) cells of adult mice are ...
Anti-CD45 antibody produced in rabbit Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered ... Prestige Antigens™-Blocking Protocol Prestige Antigens and the corresponding Prestige Antibodies are recommended to be used ... Anti-CD45 antibody produced in rabbit Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered ...
Mouse Monoclonal Antibody Shop CD45 Mouse anti-Rat, Brilliant Violet 510, Clone: OX-1, BD Optibuild™ ... CD45 Mouse anti-Rat, Brilliant Violet 510, Clone: OX-1, BD Optibuild - ... The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction. ... The OX-1 antibody recognizes all molecular forms of CD45 (Leukocyte Common Antigen) on all hematopoietic cells except ...
Shop online for a wide selection of CD45 Rat anti-Mouse, APC-eFluor(T) 780, Clone: 30-F11, eBioscience Rat Monoclonal Antibody ... CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to ... CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family ... CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all ...
leukocyte common antigen (CLA; CD45: %), and stem/progenitor (CD34: %; CD73: , and CD105: %) markers immediately postthaw. ... Compared to the immunophenotype of freshly thawed cells, the expression of antigens associated with all of the lineages ... Throughout a one-week period, adipose-derived SVF cells within the ObaGel constructs continued to express surface antigens ... the expressions of antigens associated with myeloid (CD19), stem/progenitor (CD34), preadipocyte (CD36), hematopoietic (CLA; ...
A deletion in the gene encoding the CD45 antigen in a patient with SCID. Journal of Immunology. 2001 Jan 15;166(2):1308-1313. ... A deletion in the gene encoding the CD45 antigen in a patient with SCID. / Tchilian, Elma Z.; Wallace, Diana L.; Wells, R. ... Fingerprint Dive into the research topics of A deletion in the gene encoding the CD45 antigen in a patient with SCID. ... title = "A deletion in the gene encoding the CD45 antigen in a patient with SCID", ...
Examples of CD45− and CD45+ cells from islets are shown in Supplementary Fig. 3H-J. From donors with type 1 diabetes (N = 6), ... Antigen presentation to T cells is mediated by antigen-presenting cells (APCs) via two classes of HLA molecules: HLA Class I, ... Single viable cells were analyzed for surface CD45 and intracellular insulin (left panels of each group). CD45−insulin+ cells ... The percentage of CD45−insulin+HLA-DR+ cells for each sample are shown in Q2 for each sample (far right columns in F and G). A ...
Buy our Recombinant mouse CD45 protein. Ab102105 is an active protein fragment produced in Escherichia coli and has been ... Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator ...
Antigen Expression CD30 +; CD45 +; EMA + (epithelial membrane antigen). Comments The cells contain two viral genomes: Epstein- ...
Antigen Expression CD30 +; CD38 +; CD45 +; CD 54 +; CD71 +; HLA-DR +; EMA + (epithelial membrane antigen); CD2 -; CD3 -; CD4 ... The cells do not express B-cell lineage restricted antigens or kappa or lambda immunoglobulin light chains or T-cell lineage- ...
Mouse monoclonal CD45 antibody [MEM-28] conjugated to APC. Validated in Flow Cyt and tested in Human. Cited in 4 publication(s ... Horejsí V et al. Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, ... Primary - Mouse Anti-CD45 antibody [MEM-28] (APC) (ab28106) Flow Cyt Protein - Recombinant human CD45 protein (Fc Chimera ... CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted ...
CD45) can rapidly induce aggregation of human peripheral blood mononuclear cell ... We recently reported that cross-linking the leukocyte common antigen ( ... The cell and molecular basis of leukocyte common antigen (CD45)- triggered, lymphocyte function-associated antigen-1-/ ... HM Lorenz, AS Lagoo, KJ Hardy; The cell and molecular basis of leukocyte common antigen (CD45)- triggered, lymphocyte function- ...
In addition, mutant embryos Mouse monoclonal to CD45.4AA9 reacts with CD45, a 180-220 kDa leukocyte common antigen (LCA). CD45 ... Tag: a 180-220 kDa leukocyte common antigen (LCA). CD45 antigen is expressed at high levels on all hematopoietic cells ...
CD45. common leukocyte antigen. all human leukocytes including lymphocytes, monocytes, granulocytes, eosinophils, and ... CD45. common leukocyte antigen. all human leukocytes including lymphocytes, monocytes, granulocytes, eosinophils, and ... Next plot with SSC-A and CD45 fluorescence intensity contains a gate selecting all CD45+ (immune) cells (blue). (B) FACS plot ... common B cell antigen. B cell development from pro-B cell to blastoid B cell and plasma B cell. Fitc. 3 µL. HIB19. ...
Rat Monoclonal Anti-CD45 Antibody (IBL-3/16) [DyLight 405]. Validated: Flow, ICC/IF, IHC, IHC-Fr, IP. Tested Reactivity: Mouse ... CD45 - Much more than just a housekeeping protein. CD45, also known as T200 or the Leukocyte Common Antigen (LCA), is encoded ... Home » CD45 » CD45 Antibodies » CD45 Antibody (IBL-3/16) [DyLight 405] (NB100-65364V) ... Blogs on CD45. Check out the latest blog posts on CD45.. The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research ...
... were used without antigen retrieval on cryosections. Hamster anti-T1α (clone 8.1.1, 1:1,000; DSHB) was used without antigen ... Lastly, there is an increase in the number of cells expressing CD45 after bleomycin (Fig. 1C). The simplest explanation is that ... After antigen retrieval (10 mM sodium citrate, pH 6, in a 2100 Retriever followed by 2M HCl at 37° for 20 min and 0.05% Trypsin ... Some S100a4-positive cells coexpressed vimentin and/or CD45 (Fig. 1 A and C and Fig. S1A). ...
CD Antigen Marker T cell. CD45 (LCA) strongly positive. CD45RO usually strongly positive ...
  • Immunohistochemical study showed positivity for leukocytic common antigen (CD45), B-cell marker (CD20) and bcl 6. (curehunter.com)
  • CD45 is known as leukocyte common antigen (LCA) and T200. (biolegend.com)
  • CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. (biolegend.com)
  • The OX-1 antibody recognizes all molecular forms of CD45 (Leukocyte Common Antigen) on all hematopoietic cells except erythrocytes. (fishersci.com)
  • Description: The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA). (fishersci.com)
  • We recently reported that cross-linking the leukocyte common antigen (CD45) can rapidly induce aggregation of human peripheral blood mononuclear cells via lymphocyte function-associated antigen-1 (LFA-1) and intercellular adhesion molecule-1 (ICAM-1) interactions. (ashpublications.org)
  • We conclude that cross-linking the leukocyte common antigen on T cells induces LFA-1--/ICAM-1--dependent T-cell--monocyte aggregation through a unique signaling pathway independent of PKC, which involves instead cAMP-/cGMP-dependent protein kinases. (ashpublications.org)
  • a 180-220 kDa leukocyte common antigen (LCA). (biomasswars.com)
  • In addition, mutant embryos Mouse monoclonal to CD45.4AA9 reacts with CD45, a 180-220 kDa leukocyte common antigen (LCA). (biomasswars.com)
  • 5B1 recognizes the human CD45 antigen, a tyrosine phosphatase also known as the leukocyte common antigen (LCA). (miltenyibiotec.com)
  • The non-restricted CD45, Leucocyte Common Antigen (LCA) consists of an extracellular sequence, proximal to the membrane, which is common to all CD45 isoforms. (beckman.com)
  • CD 45 is also referred to as Leucocyte Common Antigen. (osu.edu)
  • The pleomorphic, large, epithelioid, and spindle-shaped neoplastic cells of both specimens showed a diffuse cytoplasmic positivity for vimentin and membranous expression of leukocyte common antigen and UCHL-1 (Figure 6). (thefreedictionary.com)
  • 4) In our case, we observed membranous positivity for leukocyte common antigen in the histiocytes that infiltrated the salivary gland, which in our opinion can be regarded as additional evidence that these cells originate from bone marrow-derived monocytes or macrophages. (thefreedictionary.com)
  • Lymphoepithelioma-like carcinoma tumor cells are immunoreactive to cytokeratin and epithelial membrane antigen and are negative for leukocyte common antigen , confirming their epithelial nature. (thefreedictionary.com)
  • Polymorphism of the lymphoid background and immunohistochemical stains using keratin and leukocyte common antigen will help make the diagnosis. (thefreedictionary.com)
  • The nuclear feature is closer to a lymphocyte, and the cells show lymphoid antigens, such as leukocyte common antigen , CD20, and CD79. (thefreedictionary.com)
  • The tumor did not stain for leukocyte common antigen , showed weak cytoplasmic positivity for vimentin and epithelial membrane antigen, and displayed strong positivity for AE1-AE3, CAM 5. (thefreedictionary.com)
  • Although not all SCCs express epithelial markers, they react strongly with CD56 and leukocyte common antigen expression is negative. (thefreedictionary.com)
  • T-cell-associated antigens (CD3 and CD45RO) as well as leukocyte common antigen (CD45) were also absent. (thefreedictionary.com)
  • 5] The RS cells in nodular lymphocyte predominance Hodgkin disease (NLPHD) variant stain positive with antibodies to leukocyte common antigen , L26, and pan-B-cell markers in contrast to the RS cells of other variants, which are Leu-M1 positive and nonreactive with pan-B-cell markers. (thefreedictionary.com)
  • The mouse monoclonal antibody HI30 recognizes all isoforms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets. (exbio.cz)
  • 0.75 mm) of superficial spreading melanoma the vast majority of tumor-infiltrating lymphocytes (TIL) belong to the CD4+ subset and frequently express CD45RA antigens. (curehunter.com)
  • CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. (biolegend.com)
  • The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes. (fishersci.com)
  • CD45-deficient mice are severely immunodeficient and have very few peripheral T lymphocytes. (aston.ac.uk)
  • Experiments using highly purified T lymphocytes showed that CD45-induced aggregation requires the presence of both T cells and monocytes. (ashpublications.org)
  • Cross- linking CD45 only on T lymphocytes, but not on monocytes, initiated cellular clustering after reconstituting to the respective untreated cell type. (ashpublications.org)
  • Flow cytometric analysis of CD45 expression on human peripheral blood lymphocytes. (bdbiosciences.com)
  • Different populations were characterized by flow cytometry analysis using a broad panel of specific monoclonal antibodies against lymphoid surface antigens that stain porcine lymphocytes. (usda.gov)
  • The non-restricted CD45 epitope is present on the surface of all human leucocytes, lymphocytes, eosinophils monocytes, basophils and neutrophils by order of decreasing level of expression. (beckman.com)
  • The lymph node processes and presents various antigens to either B or T lymphocytes. (medscape.com)
  • Indeed lymphocytes lacking CD45 show significant hyporesponsiveness to antigen receptor cross-linking attributed to a failure to appropriately activate SFK ( 29 ). (rupress.org)
  • The CD45 antigen , expressed on the T cell surface, presents two different isoforms, RA and RO. (thefreedictionary.com)
  • The CD45 antigens occur as multiple isoforms that result from alternative mRNA splicing and differential usage of three exons. (curehunter.com)
  • The clone QA17A26 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45. (biolegend.com)
  • The CD45 isoforms detected in rat are cell type-, maturation-, and activation state-specific. (fishersci.com)
  • The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction. (fishersci.com)
  • CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. (fishersci.com)
  • CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. (fishersci.com)
  • CD45 protein exists in multiple isoforms as a result of alternative splicing, and these isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains. (thermofisher.com)
  • The CD45 molecule is required for T and B cell activation and is expressed in at least five isoforms depending on the differentiation status of the cell. (miltenyibiotec.com)
  • The CD45 antibody recognizes a common epitope of all CD45 isoforms. (miltenyibiotec.com)
  • The CD45 molecules are single chain integral membrane proteins comprising at least five isoforms ranging from 180 to 220 kDa. (beckman.com)
  • All the monoclonal antibodies that belong to the CD45 cluster react with this part of the antigen and are able to recognize all CD45 isoforms. (beckman.com)
  • Combinations of CD45 isoforms are crucial for immune function and disease. (exbio.cz)
  • Anti-CD45 is a mixture of two monoclonal antibodies, clones 2B11 and PD7/26, directed against different epitopes. (agilent.com)
  • Each population was characterized using monoclonal antibodies to cell surface antigens (CD21,CD4, CD8, DP CD4/CD8, CD16, CD21,gdTCR receptor, CD16,and SWC3)and to activation surface antigens(SLA-DQ and CD25 mean channels). (usda.gov)
  • CD45 antibodies react with leucocyte progenitors in bone marrow. (beckman.com)
  • Toxins attached to growth factors, antibodies and other cell targeting molecules can be used to kill harmful cells bearing specific receptors or antigens (Pastan et al. (google.com)
  • The antibodies of clone 4-18 recognize an antigen on granulocyctes. (uni-muenchen.de)
  • No group of leucocytes and no antigen could be assigned to the other antibodies. (uni-muenchen.de)
  • Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). (exbio.cz)
  • Human Protein Tyrosine Phosphatase CD45 (PTPRC) Antigen Immunoaffinity Purified Polyclonal antibody storage GENTAUR recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of Human Protein Tyrosine Phosphatase CD45 (PTPRC) Antigen Immunoaffinity Purified Polyclonal. (antibody-antibodies.com)
  • We report here that a homozygous 6-bp deletion in the gene encoding CD45 (PTPRC, gene map locus 1q31-32), which results in a loss of glutamic acid 339 and tyrosine 340 in the first fibronectin type III module of the extracellular domain of CD45, is associated with failure of surface expression of CD45 and SCID. (aston.ac.uk)
  • CD45, also known as PTPRC, is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells. (sigmaaldrich.com)
  • The protein encoded by this gene was identified as a transmembrane phosphoprotein specifically associated with tyrosine phosphatase PTPRC/CD45, a key regulator of T- and B-lymphocyte activation. (wikipedia.org)
  • C57BL/6 mouse Splenocytes were stained with purified CD45 (clone QA17A26) (Filled histogram) or mouse IgG1, κ purified isotype control (open histogram) and then stained with PE-rat anti-mouse IgG1. (biolegend.com)
  • Clone 2B11 was clustered as anti-CD45 at the Third International Workshop and Conference on Human Leucocyte Differentiation Antigens and reacts with all the known isotypes of the CD45 family (6). (agilent.com)
  • Anti-CD45 (mouse) Antibody, PE, clone 30-F11 is a highly specific rat monoclonal antibody, that targets CD45 & has been tested in Flow Cytometry, IHC, Activity Assay, IP & western blotting. (sigmaaldrich.com)
  • A murine IgG1 anti-CD45 monoclonal antibody (MoAb) with immunotherapeutic activity. (cancer.gov)
  • CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. (fishersci.com)
  • CD45 is absent on non-hematopoietic cell lines, normal and maligt, non-hematopoietic tissues. (fishersci.com)
  • CD45 is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. (thermofisher.com)
  • 3-5 It has been possible to phenotypically characterize normal primitive hematopoietic cells using cell sorting combined with functional assays and to show that the expression or lack of expression of specific cell-surface antigens correlates with the maturation state of cells within the normal hematopoietic hierarchy. (bloodjournal.org)
  • The transmembrane protein tyrosine phosphatase CD45 is expressed on all hematopoietic cells including NK cells and appears to function by dephosphorylating the COOH-terminal tyrosine of SFKs, leading to a conformation that favors autophosphorylation of the catalytic tyrosine ( 26 - 28 ). (rupress.org)
  • Although bone marrow cells expressing GFP under the ubiquitously expressed β-actin promoter efficiently engrafted the pancreas of normal and hyperglycemic mice, virtually all expressed CD45 and Mac-1/Gr-1, demonstrating that they adopt a hematopoietic rather than β-cell fate, a finding further substantiated by the complete absence of GFP + cells expressing insulin and the β-cell transcription factors pancreatic duodenal homeobox factor-1 and homeodomain protein. (diabetesjournals.org)
  • CD45 is a transmembrane glycoprotein expressed on most nucleated cells of hematopoetic origin. (agilent.com)
  • CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. (fishersci.com)
  • CD45 is a protein antigen expressed on all haemopoietic white cells and "gating" the CD45 antigen positive cells in each sample was performed as part of the DHR assay in this study. (thefreedictionary.com)
  • Lack of CD45 antigen on blast cells in childhood acute lymphoblastic leukemia is associated with chromosomal hyperdiploidy and other favorable prognostic features. (thefreedictionary.com)
  • The alterations of CD45RA and CD45RO antigens expression on T helper cells in prediabetics suggest the significant role of naive or/and memory CD4+ T cell subsets in the pathogenesis of diabetes type 1. (curehunter.com)
  • The CD45 tyrosine phosphatase regulates specific pools of antigen receptor-associated p59fyn and CD4-associated p56lck tyrosine in human T-cells. (nih.gov)
  • A newly isolated T-cell line (CB1) derived from a T-acute lymphoblastic leukaemia (T-ALL) patient contained cells (40% of total) which did not express the CD45 phosphotyrosine phosphatase. (nih.gov)
  • The cells were sorted into CD45- and CD45+ populations and shown to be clonal in origin. (nih.gov)
  • T-cell receptor (TCR) cross-linking or coligation of the TCR with its CD4/CD8 co-receptors induced tyrosine phosphorylation and calcium signals in CD45+ but not in CD45- cells. (nih.gov)
  • Unexpectedly, whole cell p56lck and p59fyn tyrosine kinase activities were not reduced in CD45- compared to CD45+ cells. (nih.gov)
  • Using this technique it was shown that cell surface CD4-p56lck kinase activity was 78% lower in CD45- than in CD45+ cells. (nih.gov)
  • Phosphorylation of TCR zeta- and gamma-chains occurred in TCR immunocomplexes from CD45+ but not CD45- cells, despite comparable levels of p59fyn and TCR proteins. (nih.gov)
  • Furthermore, TCR-associated tyrosine kinase activity towards an exogenous substrate was 84% lower in CD45- than in CD45+ cells. (nih.gov)
  • Addition of recombinant p59fyn to TCR immunocomplexes isolated from CD45-cells restored the phosphorylation of the TCR zeta- and gamma-chains. (nih.gov)
  • Induction of CD45 isoform switch in murine B cells by antigen receptor stimulation and by phorbol myristate acetate and ionomycin. (researchmap.jp)
  • The CD45 phosphotyrosine phosphatase is one of the most abundant glycoproteins expressed on immune cells. (nih.gov)
  • Subsets of rat CD4+ T cells defined by their differential expression of variants of the CD45 antigen: developmental relationships and in vitro and in vivo functions. (ox.ac.uk)
  • Numerous CD45(+) cells expressed the LEC marker podoplanin and were obviously integrated into the lining of lymphatic capillaries. (nih.gov)
  • This indicates that, similar to inflammation-induced lymphangiogenesis in man, circulating CD45(+) cells of adult mice are capable of initiating lymphangiogenesis and of adopting a lymphvasculogenic cellular differentiation program. (nih.gov)
  • CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. (fishersci.com)
  • These β-cells also expressed mRNA for Class II and Class II antigen presentation pathway components, but lacked the macrophage marker CD68. (diabetesjournals.org)
  • β-Cell surface expression of HLA Class II was detected on a portion of CD45 − insulin + β-cells from donors with type 1 diabetes by immunofluorescence and flow cytometry. (diabetesjournals.org)
  • Antigen presentation to T cells is mediated by antigen-presenting cells (APCs) via two classes of HLA molecules: HLA Class I, recognized by CD8 + -expressing T cells (Class I is present on nearly all nucleated cells), and HLA Class II, recognized by CD4 + -expressing T cells. (diabetesjournals.org)
  • The cells do not express B-cell lineage restricted antigens or kappa or lambda immunoglobulin light chains or T-cell lineage-restricted antigens. (atcc.org)
  • The cells do express activation antigens. (atcc.org)
  • By several criteria, CD45-induced clustering of T cells to autologous monocytes was shown to be Fc-receptor--independent. (ashpublications.org)
  • Indeed, during the first 2-4 days of an immune response all antigen-specific-cells dispersed throughout the body are retained in the restricted site where the antigen is first presented (a phenomenon named lymphocyte trapping). (frontiersin.org)
  • Recent studies suggested that this local retention was due to the formation of stable interactions between antigen-specific T cells and the antigen-presenting cells (APCs). (frontiersin.org)
  • These stable interactions would lead to T cell activation and the subsequent down-regulation of the sphingosine-1-phosphate (S1P) receptor S1P 1 at the T cell surface, preventing antigen-specific T cells to egress the lymphoid organ ( 6 ). (frontiersin.org)
  • However, these events only explain why antigen-specific-cells remain in contact with the APCs presenting the antigen. (frontiersin.org)
  • In these circumstances, some circulating antigen-specific-cells may fail to contact these rare APCs, unless their transit time through the draining lymph node (DLNs) is considerably modified. (frontiersin.org)
  • Abnormal numbers of cells expressing this antigen or aberrant expression levels of the antigen can be expected in some disease states. (miltenyibiotec.com)
  • To further define the mechanisms of CD45 action on Lyn, we metabolically labeled Lyn from CD45-positive and -negative WEHI-231 cells and analyzed cyanogen bromide fragments by SDS-PAGE analysis. (jimmunol.org)
  • In CD45-negative cells, two bands at 8.2 and 4.1 kDa were phosphorylated in the absence of B cell Ag receptor (BCR) ligation. (jimmunol.org)
  • Furthermore, coimmunoprecipitations using anti-Igα Ab demonstrated that Lyn associated with the resting BCR was constitutively phosphorylated and activated in CD45-negative cells. (jimmunol.org)
  • CD45 has been implicated in both T and B cell activation based on experiments using CD45-deficient cells ( 13 , 14 , 15 , 16 , 17 ) and cells from CD45 gene-targeted mice ( 18 , 19 ). (jimmunol.org)
  • It has been shown that CD45 in T cells dephosphorylates Lck and Fyn at the COOH-terminal negative regulatory sites, Tyr 505 ( 20 , 21 , 22 , 23 , 24 ) and Tyr 531 ( 25 , 26 , 27 ), respectively. (jimmunol.org)
  • We have previously demonstrated that in CD45-deficient clones generated from immature WEHI-231 cells, Lyn is selectively hyperphosphorylated and activated in the absence of BCR ligation. (jimmunol.org)
  • BCR-induced Ca 2+ mobilization, growth arrest, and apoptosis were also negatively regulated by CD45 in WEHI-231 cells ( 16 ). (jimmunol.org)
  • In contrast, CD45 exerted positive, crucial effects on BCR-induced growth arrest and, to a lesser extent, BCR-induced tyrosine phosphorylation in mature BAL-17 B cells ( 17 ). (jimmunol.org)
  • Leukemic blast peripheral blood cells were evaluated for expression of CD34 and Thy-1 (CD90) antigens. (bloodjournal.org)
  • 1 2 Subsequent to transformation some evidence suggests that cells from the malignant clone undergo further differentiation, creating a hierarchy of cells that differ in their expression of cell-surface antigens and may also differ in their proliferative potential. (bloodjournal.org)
  • 18 The nonobese diabetic (NOD)/SCID strain is characterized by a functional deficit in NK cells, absence of circulating complement and defective antigen-presenting cells. (bloodjournal.org)
  • Efficient T cell activation is dependent on the intimate contact between antigen-presenting cells (APCs) and T cells. (rupress.org)
  • The engagement of the B7 family of molecules on APCs with CD28 and CD152 (cytotoxic T lymphocyte-associated antigen 4 [CTLA-4]) receptors on T cells delivers costimulatory signal(s) important in T cell activation. (rupress.org)
  • The CD3 antigen is present on the cell membrane of normal T cells and is expressed in the majority of T-cell neoplasms. (osu.edu)
  • Also referred to as MART1 (Melanoma Antigen Recognized by T cells). (osu.edu)
  • Factor VIII-realted antigen (vonWillebrand factor) is present in endothelial cells and in the cytoplasm of megakaryocytes. (osu.edu)
  • both variants show intraepidermal, highly activated, proliferating T cells with variable loss of pan-T-cell antigens, contrasting with nonactivated dermal reactive T cells. (curehunter.com)
  • Among different MRD techniques, flow cytometry provides additional information about antigen expression on tumor cells, which could potentially contribute to stratify MRD-positive patients. (nature.com)
  • Adoptive transfer of T cells engineered to express a chimeric antigen receptor (CAR) is an effective therapy for select lymphomas. (sciencemag.org)
  • Among the possible design limitations of CAR-T cells for cancer are unwanted tonic (antigen-independent) signaling and off-target activation. (sciencemag.org)
  • Memory CD4+ T cells are preferentially infected by HIV-1 compared to naïve cells.Conversely, the lower cortical actin density in naïve CD4+ T cells restricted viral antigen transfer and consequently HIV-1 infection.In conclusion, the cortical actin density differentially affects the susceptibility to HIV-1 infection in naïve and memory CD4+ T cells by modulating the efficiency of HIV antigen internalization. (nih.gov)
  • Here, we evaluate the role of the cortical actin in cell-to-cell transfer of virus antigens and infection of target CD4+ T cells. (nih.gov)
  • Thus, the higher cortical actin density of memory CD4+ T cells was associated to increased efficiency of HIV-antigen internalization and the establishment of a productive infection. (nih.gov)
  • Conversely, the lower cortical actin density in naïve CD4+ T cells restricted viral antigen transfer and consequently HIV-1 infection. (nih.gov)
  • Discordant expression of antigens between intraepidermal and intradermal T cells in mycosis fungoides. (springer.com)
  • Stimulation via the major histocompatibility complex I-binding receptor, Ly49D on CD45 −/− primary NK cells resulted in the activation of phosphoinositide-3-kinase and normal cytotoxicity but failed to elicit a range of cytokines and chemokines. (rupress.org)
  • These data, supported by analogous findings after CD16 and NKG2D stimulation of CD45 −/− primary NK cells, place CD45 upstream of SFK in NK cells after stimulation via immunoreceptor tyrosine-based activation motif-containing receptors. (rupress.org)
  • Antibody bound to soluble or membrane antigens can bind CD16 on NK cells leading to antibody-dependent cell cytotoxicity (ADCC) and secretion of IFN-γ and GM-CSF ( 8 , 9 ). (rupress.org)
  • In order to broad- en the array of tools for cell-based autologous therapies, we iso- lated a novel renewable stem cell population from the adult testes that has characteristics of MSCs, termed gonadal stem cells (GSCs). (slideshare.net)
  • The key to success in this study was use of a radiolabeled antibody that has therapeutic iodine 131 attached and is designed to target leukemic bloods cells that carry a marker on the surface of the cell known as CD45. (innovations-report.com)
  • Delivered intravenously, the radiation looks for the CD45 antigen receptor on the surface of blood cells. (innovations-report.com)
  • We evaluated multiple signaling pathways in antigen-defined subpopulations in primary AML cells with FLT3-ITD mutations. (mendeley.com)
  • Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis. (exbio.cz)
  • Bijian K, Zhang L, Shen SH: Collagen-mediated survival signaling is modulated by CD45 in Jurkat T cells. (exbio.cz)
  • Molecular modeling suggests that tyrosine 340 is crucial for the structural integrity of CD45 protein. (aston.ac.uk)
  • Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. (abcam.com)
  • When comparing intracellular signaling in leukocyte aggregation induced by CD45 cross-linking versus phorbol myristate-12-13-acetate (PMA) treatment, the former was found to be fivefold to 10-fold more sensitive to H-8, a reagent that effectively blocks cAMP- and cGMP- dependent protein kinases. (ashpublications.org)
  • CD45 a receptor type protein tyrosine phosphatase protein. (phosphosite.org)
  • Using CD45-deficient clones from the immature B cell line, WEHI-231, we previously demonstrated that CD45 selectively dephosphorylates the Src-family protein tyrosine kinase Lyn and inhibits its kinase activity. (jimmunol.org)
  • Extensive analyses have demonstrated that one of the crucial enhancers of BCR signaling is the receptor-type protein tyrosine phosphatase, CD45 ( 8 , 9 , 10 , 11 , 12 ). (jimmunol.org)
  • CD45 antigen, a receptor protein-tyrosine phosphatase essential for T cell development and lymphocyte activation, is expressed on virtually all leukocytes. (cancer.gov)
  • CD45 plays a key role in TCR and BCR signal transduction. (biolegend.com)
  • CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions. (fishersci.com)
  • In addition to its role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. (thermofisher.com)
  • Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. (thermofisher.com)
  • In this study we investigate the role of the cell surface phosphatase CD45 in NK cell development and intracellular signaling from activating receptors. (rupress.org)
  • Flow Cytometry Analysis: 0.25 μg of this antibody detected CD45 in one million C57BL/6 mouse splenocytes. (sigmaaldrich.com)
  • These cell surface antigens can comprise up to 10% of the membrane CD45 proteins. (thefreedictionary.com)
  • Human whole blood was stained with either BD Horizon™ BUV395 Mouse Anti-Human CD45 antibody (Cat. (bdbiosciences.com)
  • Despite the benefits of chimeric antigen receptor (CAR)-T cell therapies against lymphoid malignancies, responses in solid tumors have been more limited and off-target toxicities have been more marked. (sciencemag.org)
  • CyTOF and conventional FCM yielded comparable results on LSC phenotypes defined by CD45, CD34, CD38, CD123, and CD99. (mendeley.com)
  • Clone 2B11 reacts with all known isotypes of the CD45 family and clone PD7/26 has been clustered as anti-CD45RB. (agilent.com)
  • This antibody reacts specifically with human CD45 and is useful for identifying human leukocytes transplanted into immunodeficient mice. (osu.edu)
  • The CD45 tyrosine phosphatase: a positive and negative regulator of immune cell function. (nih.gov)
  • CD45 is an abundant transmembrane tyrosine phosphatase, expressed on all leukocytes, and is required for efficient lymphocyte signaling. (aston.ac.uk)
  • CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. (biolegend.com)
  • This antibody recognizes the antigen presenting on the cell surface membrane of tissue macrophages. (osu.edu)
  • Tissue Antigens. (wikipedia.org)
  • Publications] Takeuchi, T, et al: 'Characterization of AE-b monoclonal antibody recogniging VHCSAGV sequence in CD45 PTPase domain' Tissue Antigens. (nii.ac.jp)
  • CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. (biolegend.com)
  • CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. (fishersci.com)
  • An essential regulator of T- and B-cell antigen receptor signaling. (phosphosite.org)
  • For example, a cell that has the receptor stem cell antigen -1, on its surface, is identified as Sca-1. (nih.gov)
  • We investigated the first SCID patient to be described with minimal cell surface expression of the leukocyte common (CD45) Ag. (aston.ac.uk)
  • Clone PD7/26 was clustered as anti-CD45RB at the Fifth International Workshop and Conference on Human Leucocyte Differentiation Antigens (7). (agilent.com)
  • White cell differentiation Antigens. (miltenyibiotec.com)
  • Our analysis of bone marrow samples from 50 patients with MDS showed aberrant expression of differentiation antigens in the myelomonocytic lineage. (bloodjournal.org)
  • Our results demonstrate that CD45 selectively regulates the pools of p59fyn and p56lck kinases which are associated with the TCR and CD4 at the cell surface. (nih.gov)
  • It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. (genecards.org)
  • Interestingly, CD45 has been associated with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45. (sigmaaldrich.com)
  • CD45 is a major component of the lymphocyte membrane. (beckman.com)
  • CD45 is present on the cell membrane of normal and neoplastic leukocytes. (osu.edu)
  • Antigens, CD45" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (childrensmercy.org)
  • or the levels of monoclonal immunoglobulins, carcinoembryonic antigen (CEA), alfa-fetoprotein (AFP), prostate specific antigen (PSA), cancer antigen 125 (CA 125) or human chorionic gonadotropin (HCG) in the bloodstream [ 2 ], [ 3 ]. (degruyter.com)
  • CD45 was also shown to dephosphorylate autophosphorylated Lyn in vitro. (jimmunol.org)
  • Beverley, Peter C. L. / A deletion in the gene encoding the CD45 antigen in a patient with SCID . (aston.ac.uk)
  • Ink4a(-/-) tumors exhibited increased CD45 expression, reflective of immune cell infiltration and upregulation of gene networks associated with the cytoskeleton, DNA damage response, and small molecule transport. (biomedsearch.com)
  • In CD45 gene-targeted clones from the chicken DT40 B cell line, Lyn was shown to be hyperphosphorylated on BCR ligation at the COOH-terminal negative regulatory residue as well as the positive regulatory tyrosine. (jimmunol.org)
  • Mice transgenic for the rat insulin promoter II gene linked to the large-T antigen of SV40 (RIPTag) develop solid β-cell tumors of the pancreas. (aacrjournals.org)
  • Taken collectively, these results suggest that CD45 keeps both BCR-associated and total cytoplasmic pools of Lyn in an inactive state, and a mechanism by which Lyn is activated by relative reduction of CD45 effect may be operative on BCR ligation. (jimmunol.org)
  • Activation by CD45 of these receptor-associated kinase pools correlates with the ability of the TCR and its coreceptors to couple to intracellular signalling pathways. (nih.gov)
  • CD45 has been shown to play an important role in T-cell activation through the antigen receptor. (sigmaaldrich.com)
  • Upon T-cell activation, CD45 recruits and dephosphorylates SKAP1 and FYN. (sigmaaldrich.com)