Substances that are recognized by the immune system and induce an immune reaction.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
Differentiation antigens found on thymocytes and on cytotoxic and suppressor T-lymphocytes. CD8 antigens are members of the immunoglobulin supergene family and are associative recognition elements in MHC (Major Histocompatibility Complex) Class I-restricted interactions.
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
Substances elaborated by bacteria that have antigenic activity.
A bifunctional enzyme that catalyzes the synthesis and HYDROLYSIS of CYCLIC ADP-RIBOSE (cADPR) from NAD+ to ADP-RIBOSE. It is a cell surface molecule which is predominantly expressed on LYMPHOID CELLS and MYELOID CELLS.
Glycoproteins found on immature hematopoietic cells and endothelial cells. They are the only molecules to date whose expression within the blood system is restricted to a small number of progenitor cells in the bone marrow.
Differentiation antigens expressed on B-lymphocytes and B-cell precursors. They are involved in regulation of B-cell proliferation.
A member of the tumor necrosis factor receptor superfamily with specificity for CD40 LIGAND. It is found on mature B-LYMPHOCYTES and some EPITHELIAL CELLS, lymphoid DENDRITIC CELLS. Evidence suggests that CD40-dependent activation of B-cells is important for generation of memory B-cells within the germinal centers. Mutations of the gene for CD40 antigen result in HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 3. Signaling of the receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
A membrane glycoprotein and differentiation antigen expressed on the surface of T-cells that binds to CD40 ANTIGENS on B-LYMPHOCYTES and induces their proliferation. Mutation of the gene for CD40 ligand is a cause of HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 1.
Unglycosylated phosphoproteins expressed only on B-cells. They are regulators of transmembrane Ca2+ conductance and thought to play a role in B-cell activation and proliferation.
Substances elaborated by viruses that have antigenic activity.
Costimulatory T-LYMPHOCYTE receptors that have specificity for CD80 ANTIGEN and CD86 ANTIGEN. Activation of this receptor results in increased T-cell proliferation, cytokine production and promotion of T-cell survival.
Acidic sulfated integral membrane glycoproteins expressed in several alternatively spliced and variable glycosylated forms on a wide variety of cell types including mature T-cells, B-cells, medullary thymocytes, granulocytes, macrophages, erythrocytes, and fibroblasts. CD44 antigens are the principle cell surface receptors for hyaluronate and this interaction mediates binding of lymphocytes to high endothelial venules. (From Abbas et al., Cellular and Molecular Immunology, 2d ed, p156)
Differentiation antigens expressed on pluripotential hematopoietic cells, most human thymocytes, and a major subset of peripheral blood T-lymphocytes. They have been implicated in integrin-mediated cellular adhesion and as signalling receptors on T-cells.
Glycolipid-anchored membrane glycoproteins expressed on cells of the myelomonocyte lineage including monocytes, macrophages, and some granulocytes. They function as receptors for the complex of lipopolysaccharide (LPS) and LPS-binding protein.
Glycoprotein members of the immunoglobulin superfamily which participate in T-cell adhesion and activation. They are expressed on most peripheral T-lymphocytes, natural killer cells, and thymocytes, and function as co-receptors or accessory molecules in the T-cell receptor complex.
Ratio of T-LYMPHOCYTES that express the CD4 ANTIGEN to those that express the CD8 ANTIGEN. This value is commonly assessed in the diagnosis and staging of diseases affecting the IMMUNE SYSTEM including HIV INFECTIONS.
Glycoproteins expressed on all mature T-cells, thymocytes, and a subset of mature B-cells. Antibodies specific for CD5 can enhance T-cell receptor-mediated T-cell activation. The B-cell-specific molecule CD72 is a natural ligand for CD5. (From Abbas et al., Cellular and Molecular Immunology, 2d ed, p156)
Antigens expressed primarily on the membranes of living cells during sequential stages of maturation and differentiation. As immunologic markers they have high organ and tissue specificity and are useful as probes in studies of normal cell development as well as neoplastic transformation.
A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.
Glycoproteins expressed on cortical thymocytes and on some dendritic cells and B-cells. Their structure is similar to that of MHC Class I and their function has been postulated as similar also. CD1 antigens are highly specific markers for human LANGERHANS CELLS.
Antibodies produced by a single clone of cells.
The 140 kDa isoform of NCAM (neural cell adhesion molecule) containing a transmembrane domain and short cytoplasmic tail. It is expressed by all lymphocytes mediating non-MHC restricted cytotoxicity and is present on some neural tissues and tumors.
Antigens expressed on the cell membrane of T-lymphocytes during differentiation, activation, and normal and neoplastic transformation. Their phenotypic characterization is important in differential diagnosis and studies of thymic ontogeny and T-cell function.
A membrane-bound or cytosolic enzyme that catalyzes the synthesis of CYCLIC ADP-RIBOSE (cADPR) from nicotinamide adenine dinucleotide (NAD). This enzyme generally catalyzes the hydrolysis of cADPR to ADP-RIBOSE, as well, and sometimes the synthesis of cyclic ADP-ribose 2' phosphate (2'-P-cADPR) from NADP.
Surface antigens expressed on myeloid cells of the granulocyte-monocyte-histiocyte series during differentiation. Analysis of their reactivity in normal and malignant myelomonocytic cells is useful in identifying and classifying human leukemias and lymphomas.
A costimulatory ligand expressed by ANTIGEN-PRESENTING CELLS that binds to CTLA-4 ANTIGEN with high specificity and to CD28 ANTIGEN with low specificity. The interaction of CD80 with CD28 ANTIGEN provides a costimulatory signal to T-LYMPHOCYTES, while its interaction with CTLA-4 ANTIGEN may play a role in inducing PERIPHERAL TOLERANCE.
Tetraspanin proteins found at high levels in cells of the lymphoid-myeloid lineage. CD53 antigens may be involved regulating the differentiation of T-LYMPHOCYTES and the activation of B-LYMPHOCYTES.
A cell adhesion protein that was originally identified as a heat stable antigen in mice. It is involved in METASTASIS and is highly expressed in many NEOPLASMS.
Zinc-binding metalloproteases that are members of the type II integral membrane metalloproteases. They are expressed by GRANULOCYTES; MONOCYTES; and their precursors as well as by various non-hematopoietic cells. They release an N-terminal amino acid from a peptide, amide or arylamide.
Any part or derivative of any protozoan that elicits immunity; malaria (Plasmodium) and trypanosome antigens are presently the most frequently encountered.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
A costimulatory ligand expressed by ANTIGEN-PRESENTING CELLS that binds to CD28 ANTIGEN with high specificity and to CTLA-4 ANTIGEN with low specificity. The interaction of CD86 with CD28 ANTIGEN provides a stimulatory signal to T-LYMPHOCYTES, while its interaction with CTLA-4 ANTIGEN may play a role in inducing PERIPHERAL TOLERANCE.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.
A tumor necrosis factor receptor subtype found in a variety of tissues and on activated LYMPHOCYTES. It has specificity for FAS LIGAND and plays a role in regulation of peripheral immune responses and APOPTOSIS. Multiple isoforms of the protein exist due to multiple ALTERNATIVE SPLICING. The activated receptor signals via a conserved death domain that associates with specific TNF RECEPTOR-ASSOCIATED FACTORS in the CYTOPLASM.
Antigens determined by leukocyte loci found on chromosome 6, the major histocompatibility loci in humans. They are polypeptides or glycoproteins found on most nucleated cells and platelets, determine tissue types for transplantation, and are associated with certain diseases.
Membrane antigens associated with maturation stages of B-lymphocytes, often expressed in tumors of B-cell origin.
High-molecular weight glycoproteins uniquely expressed on the surface of LEUKOCYTES and their hemopoietic progenitors. They contain a cytoplasmic protein tyrosine phosphatase activity which plays a role in intracellular signaling from the CELL SURFACE RECEPTORS. The CD45 antigens occur as multiple isoforms that result from alternative mRNA splicing and differential usage of three exons.
Process of classifying cells of the immune system based on structural and functional differences. The process is commonly used to analyze and sort T-lymphocytes into subsets based on CD antigens by the technique of flow cytometry.
Substances of fungal origin that have antigenic activity.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The major group of transplantation antigens in the mouse.
A 67-kDa sialic acid binding lectin that is specific for MYELOID CELLS and MONOCYTE-MACROPHAGE PRECURSOR CELLS. This protein is the smallest siglec subtype and contains a single immunoglobulin C2-set domain. It may play a role in intracellular signaling via its interaction with SHP-1 PROTEIN-TYROSINE PHOSPHATASE and SHP-2 PROTEIN-TYROSINE PHOSPHATASE.
Any part or derivative of a helminth that elicits an immune reaction. The most commonly seen helminth antigens are those of the schistosomes.
Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.
Cell-surface glycoprotein beta-chains that are non-covalently linked to specific alpha-chains of the CD11 family of leukocyte-adhesion molecules (RECEPTORS, LEUKOCYTE-ADHESION). A defect in the gene encoding CD18 causes LEUKOCYTE-ADHESION DEFICIENCY SYNDROME.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
A member of the tumor necrosis factor receptor superfamily that may play a role in the regulation of NF-KAPPA B and APOPTOSIS. They are found on activated T-LYMPHOCYTES; B-LYMPHOCYTES; NEUTROPHILS; EOSINOPHILS; MAST CELLS and NK CELLS. Overexpression of CD30 antigen in hematopoietic malignancies make the antigen clinically useful as a biological tumor marker. Signaling of the receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
Glycoproteins found on the membrane or surface of cells.
A critical subpopulation of regulatory T-lymphocytes involved in MHC Class I-restricted interactions. They include both cytotoxic T-lymphocytes (T-LYMPHOCYTES, CYTOTOXIC) and CD8+ suppressor T-lymphocytes.
Sites on an antigen that interact with specific antibodies.
A subtype of tetraspanin proteins that play a role in cell adhesion, cell motility, and tumor metastasis. CD9 antigens take part in the process of platelet activation and aggregation, the formation of paranodal junctions in neuronal tissue, and the fusion of sperm with egg.
A glycoprotein that is secreted into the luminal surface of the epithelia in the gastrointestinal tract. It is found in the feces and pancreaticobiliary secretions and is used to monitor the response to colon cancer treatment.
A subclass of HLA-D antigens that consist of alpha and beta chains. The inheritance of HLA-DR antigens differs from that of the HLA-DQ ANTIGENS and HLA-DP ANTIGENS.
A trisaccharide antigen expressed on glycolipids and many cell-surface glycoproteins. In the blood the antigen is found on the surface of NEUTROPHILS; EOSINOPHILS; and MONOCYTES. In addition, CD15 antigen is a stage-specific embryonic antigen.
Those proteins recognized by antibodies from serum of animals bearing tumors induced by viruses; these proteins are presumably coded for by the nucleic acids of the same viruses that caused the neoplastic transformation.
Established cell cultures that have the potential to propagate indefinitely.
A sialic acid-rich protein and an integral cell membrane mucin. It plays an important role in activation of T-LYMPHOCYTES.
Leukocyte differentiation antigens and major platelet membrane glycoproteins present on MONOCYTES; ENDOTHELIAL CELLS; PLATELETS; and mammary EPITHELIAL CELLS. They play major roles in CELL ADHESION; SIGNAL TRANSDUCTION; and regulation of angiogenesis. CD36 is a receptor for THROMBOSPONDINS and can act as a scavenger receptor that recognizes and transports oxidized LIPOPROTEINS and FATTY ACIDS.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A group of three different alpha chains (CD11a, CD11b, CD11c) that are associated with an invariant CD18 beta chain (ANTIGENS, CD18). The three resulting leukocyte-adhesion molecules (RECEPTORS, LEUKOCYTE ADHESION) are LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN-1; MACROPHAGE-1 ANTIGEN; and ANTIGEN, P150,95.
Large, transmembrane, non-covalently linked glycoproteins (alpha and beta). Both chains can be polymorphic although there is more structural variation in the beta chains. The class II antigens in humans are called HLA-D ANTIGENS and are coded by a gene on chromosome 6. In mice, two genes named IA and IE on chromosome 17 code for the H-2 antigens. The antigens are found on B-lymphocytes, macrophages, epidermal cells, and sperm and are thought to mediate the competence of and cellular cooperation in the immune response. The term IA antigens used to refer only to the proteins encoded by the IA genes in the mouse, but is now used as a generic term for any class II histocompatibility antigen.
A group of antigens that includes both the major and minor histocompatibility antigens. The former are genetically determined by the major histocompatibility complex. They determine tissue type for transplantation and cause allograft rejections. The latter are systems of allelic alloantigens that can cause weak transplant rejection.
Small glycoproteins found on both hematopoietic and non-hematopoietic cells. CD59 restricts the cytolytic activity of homologous complement by binding to C8 and C9 and blocking the assembly of the membrane attack complex. (From Barclay et al., The Leukocyte Antigen FactsBook, 1993, p234)
IMMUNOGLOBULINS on the surface of B-LYMPHOCYTES. Their MESSENGER RNA contains an EXON with a membrane spanning sequence, producing immunoglobulins in the form of type I transmembrane proteins as opposed to secreted immunoglobulins (ANTIBODIES) which do not contain the membrane spanning segment.
Nuclear antigen with a role in DNA synthesis, DNA repair, and cell cycle progression. PCNA is required for the coordinated synthesis of both leading and lagging strands at the replication fork during DNA replication. PCNA expression correlates with the proliferation activity of several malignant and non-malignant cell types.
Oligosaccharide antigenic determinants found principally on NK cells and T-cells. Their role in the immune response is poorly understood.
A transmembrane protein belonging to the tumor necrosis factor superfamily that specifically binds to CD27 ANTIGEN. It is found on activated T-LYMPHOCYTES; B-LYMPHOCYTES; and DENDRITIC CELLS where it plays a role in stimulating the proliferation of CD4-POSITIVE T-LYMPHOCYTES and CD8-POSITIVE T-LYMPHOCYTES.
A ubiquitously expressed complement receptor that binds COMPLEMENT C3B and COMPLEMENT C4B and serves as a cofactor for their inactivation. CD46 also interacts with a wide variety of pathogens and mediates immune response.
A class of animal lectins that bind to carbohydrate in a calcium-dependent manner. They share a common carbohydrate-binding domain that is structurally distinct from other classes of lectins.
Glycoproteins with a wide distribution on hematopoietic and non-hematopoietic cells and strongly expressed on macrophages. CD58 mediates cell adhesion by binding to CD2; (ANTIGENS, CD2); and this enhances antigen-specific T-cell activation.
55-kDa antigens found on HELPER-INDUCER T-LYMPHOCYTES and on a variety of other immune cell types. CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in MAJOR HISTOCOMPATIBILITY COMPLEX class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as INTERLEUKIN-15 receptors and bind to the HIV receptors, binding directly to the HIV ENVELOPE PROTEIN GP120.
A ubiquitously expressed membrane glycoprotein. It interacts with a variety of INTEGRINS and mediates responses to EXTRACELLULAR MATRIX PROTEINS.
A CD antigen that contains a conserved I domain which is involved in ligand binding. When combined with CD18 the two subunits form MACROPHAGE-1 ANTIGEN.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A glycoprotein that is a kallikrein-like serine proteinase and an esterase, produced by epithelial cells of both normal and malignant prostate tissue. It is an important marker for the diagnosis of prostate cancer.
An integrin alpha subunit of approximately 150-kDa molecular weight. It is expressed at high levels on monocytes and combines with CD18 ANTIGEN to form the cell surface receptor INTEGRIN ALPHAXBETA2. The subunit contains a conserved I-domain which is characteristic of several of alpha integrins.
The lipopolysaccharide-protein somatic antigens, usually from gram-negative bacteria, important in the serological classification of enteric bacilli. The O-specific chains determine the specificity of the O antigens of a given serotype. O antigens are the immunodominant part of the lipopolysaccharide molecule in the intact bacterial cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
A specific HLA-A surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-A*02 allele family.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Progenitor cells from which all blood cells derive.
The number of CD4-POSITIVE T-LYMPHOCYTES per unit volume of BLOOD. Determination requires the use of a fluorescence-activated flow cytometer.
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
Carbohydrate antigens expressed by malignant tissue. They are useful as tumor markers and are measured in the serum by means of a radioimmunoassay employing monoclonal antibodies.
GPI-linked membrane proteins broadly distributed among hematopoietic and non-hematopoietic cells. CD55 prevents the assembly of C3 CONVERTASE or accelerates the disassembly of preformed convertase, thus blocking the formation of the membrane attack complex.
Cell adhesion molecules present on virtually all monocytes, platelets, and granulocytes. CD31 is highly expressed on endothelial cells and concentrated at the junctions between them.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Membrane glycoproteins consisting of an alpha subunit and a BETA 2-MICROGLOBULIN beta subunit. In humans, highly polymorphic genes on CHROMOSOME 6 encode the alpha subunits of class I antigens and play an important role in determining the serological specificity of the surface antigen. Class I antigens are found on most nucleated cells and are generally detected by their reactivity with alloantisera. These antigens are recognized during GRAFT REJECTION and restrict cell-mediated lysis of virus-infected cells.
Tetraspanin proteins that are involved in a variety of cellular functions including BASEMENT MEMBRANE assembly, and in the formation of a molecular complexes on the surface of LYMPHOCYTES.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
A member of the tumor necrosis factor receptor superfamily that is specific for 4-1BB LIGAND. It is found in a variety of immune cell types including activated T-LYMPHOCYTES; NATURAL KILLER CELLS; and DENDRITIC CELLS. Activation of the receptor on T-LYMPHOCYTES plays a role in their expansion, production of cytokines and survival. Signaling by the activated receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Proteins prepared by recombinant DNA technology.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.
Polymorphic class I human histocompatibility (HLA) surface antigens present on almost all nucleated cells. At least 20 antigens have been identified which are encoded by the A locus of multiple alleles on chromosome 6. They serve as targets for T-cell cytolytic responses and are involved with acceptance or rejection of tissue/organ grafts.
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).
Receptors present on activated T-LYMPHOCYTES and B-LYMPHOCYTES that are specific for INTERLEUKIN-2 and play an important role in LYMPHOCYTE ACTIVATION. They are heterotrimeric proteins consisting of the INTERLEUKIN-2 RECEPTOR ALPHA SUBUNIT, the INTERLEUKIN-2 RECEPTOR BETA SUBUNIT, and the INTERLEUKIN RECEPTOR COMMON GAMMA-CHAIN.
Sets of cell surface antigens located on BLOOD CELLS. They are usually membrane GLYCOPROTEINS or GLYCOLIPIDS that are antigenically distinguished by their carbohydrate moieties.
Those hepatitis B antigens found on the surface of the Dane particle and on the 20 nm spherical and tubular particles. Several subspecificities of the surface antigen are known. These were formerly called the Australia antigen.
Ubiquitously-expressed tetraspanin proteins that are found in late ENDOSOMES and LYSOSOMES and have been implicated in intracellular transport of proteins.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Tetraspanin proteins found associated with LAMININ-binding INTEGRINS. The CD151 antigens may play a role in the regulation of CELL MOTILITY.
A component of the B-cell antigen receptor that is involved in B-cell antigen receptor heavy chain transport to the PLASMA MEMBRANE. It is expressed almost exclusively in B-LYMPHOCYTES and serves as a useful marker for B-cell NEOPLASMS.
An encapsulated lymphatic organ through which venous blood filters.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Human immune-response or Class II antigens found mainly, but not exclusively, on B-lymphocytes and produced from genes of the HLA-D locus. They are extremely polymorphic families of glycopeptides, each consisting of two chains, alpha and beta. This group of antigens includes the -DR, -DQ and -DP designations, of which HLA-DR is most studied; some of these glycoproteins are associated with certain diseases, possibly of immune etiology.
A membrane-bound tumor necrosis family member found primarily on activated T-LYMPHOCYTES that binds specifically to CD30 ANTIGEN. It may play a role in INFLAMMATION and immune regulation.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
A class of enzymes involved in the hydrolysis of the N-glycosidic bond of nitrogen-linked sugars.
A form of undifferentiated malignant LYMPHOMA usually found in central Africa, but also reported in other parts of the world. It is commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. B-cell antigens are expressed on the immature cells that make up the tumor in virtually all cases of Burkitt lymphoma. The Epstein-Barr virus (HERPESVIRUS 4, HUMAN) has been isolated from Burkitt lymphoma cases in Africa and it is implicated as the causative agent in these cases; however, most non-African cases are EBV-negative.
Molecules on the surface of B- and T-lymphocytes that recognize and combine with specific antigens.
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.
An alpha-integrin subunit found on lymphocytes, granulocytes, macrophages and monocytes. It combines with the integrin beta2 subunit (CD18 ANTIGEN) to form LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN-1.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Antigens of the virion of the HEPATITIS B VIRUS or the Dane particle, its surface (HEPATITIS B SURFACE ANTIGENS), core (HEPATITIS B CORE ANTIGENS), and other associated antigens, including the HEPATITIS B E ANTIGENS.
The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.
The processes triggered by interactions of ANTIBODIES with their ANTIGENS.
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
Mice homozygous for the mutant autosomal recessive gene "scid" which is located on the centromeric end of chromosome 16. These mice lack mature, functional lymphocytes and are thus highly susceptible to lethal opportunistic infections if not chronically treated with antibiotics. The lack of B- and T-cell immunity resembles severe combined immunodeficiency (SCID) syndrome in human infants. SCID mice are useful as animal models since they are receptive to implantation of a human immune system producing SCID-human (SCID-hu) hematochimeric mice.
Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
A heterogeneous group of immunocompetent cells that mediate the cellular immune response by processing and presenting antigens to the T-cells. Traditional antigen-presenting cells include MACROPHAGES; DENDRITIC CELLS; LANGERHANS CELLS; and B-LYMPHOCYTES. FOLLICULAR DENDRITIC CELLS are not traditional antigen-presenting cells, but because they hold antigen on their cell surface in the form of IMMUNE COMPLEXES for B-cell recognition they are considered so by some authors.
The type species of LYMPHOCRYPTOVIRUS, subfamily GAMMAHERPESVIRINAE, infecting B-cells in humans. It is thought to be the causative agent of INFECTIOUS MONONUCLEOSIS and is strongly associated with oral hairy leukoplakia (LEUKOPLAKIA, HAIRY;), BURKITT LYMPHOMA; and other malignancies.
T-cell receptors composed of CD3-associated alpha and beta polypeptide chains and expressed primarily in CD4+ or CD8+ T-cells. Unlike immunoglobulins, the alpha-beta T-cell receptors recognize antigens only when presented in association with major histocompatibility (MHC) molecules.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Class I human histocompatibility (HLA) surface antigens encoded by more than 30 detectable alleles on locus B of the HLA complex, the most polymorphic of all the HLA specificities. Several of these antigens (e.g., HLA-B27, -B7, -B8) are strongly associated with predisposition to rheumatoid and other autoimmune disorders. Like other class I HLA determinants, they are involved in the cellular immune reactivity of cytolytic T lymphocytes.
The altered state of immunologic responsiveness resulting from initial contact with antigen, which enables the individual to produce antibodies more rapidly and in greater quantity in response to secondary antigenic stimulus.
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
The phenomenon of target cell destruction by immunologically active effector cells. It may be brought about directly by sensitized T-lymphocytes or by lymphoid or myeloid "killer" cells, or it may be mediated by cytotoxic antibody, cytotoxic factor released by lymphoid cells, or complement.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
A melanosome-specific protein that plays a role in the expression, stability, trafficking, and processing of GP100 MELANOMA ANTIGEN, which is critical to the formation of Stage II MELANOSOMES. The protein is used as an antigen marker for MELANOMA cells.
A widely distributed cell surface transmembrane glycoprotein that stimulates the synthesis of MATRIX METALLOPROTEINASES. It is found at high levels on the surface of malignant NEOPLASMS and may play a role as a mediator of malignant cell behavior.
A general term for various neoplastic diseases of the lymphoid tissue.
An albumin obtained from the white of eggs. It is a member of the serpin superfamily.
Antigens associated with specific proteins of the human adult T-cell immunodeficiency virus (HIV); also called HTLV-III-associated and lymphadenopathy-associated virus (LAV) antigens.
An inhibitory T CELL receptor that is closely related to CD28 ANTIGEN. It has specificity for CD80 ANTIGEN and CD86 ANTIGEN and acts as a negative regulator of peripheral T cell function. CTLA-4 antigen is believed to play role in inducing PERIPHERAL TOLERANCE.
A promyelocytic cell line derived from a patient with ACUTE PROMYELOCYTIC LEUKEMIA. HL-60 cells lack specific markers for LYMPHOID CELLS but express surface receptors for FC FRAGMENTS and COMPLEMENT SYSTEM PROTEINS. They also exhibit phagocytic activity and responsiveness to chemotactic stimuli. (From Hay et al., American Type Culture Collection, 7th ed, pp127-8)
A widely expressed transmembrane glycoprotein that functions as a METASTASIS suppressor protein. It is underexpressed in a variety of human NEOPLASMS.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
A group of differentiation surface antigens, among the first to be discovered on thymocytes and T-lymphocytes. Originally identified in the mouse, they are also found in other species including humans, and are expressed on brain neurons and other cells.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
The specific failure of a normally responsive individual to make an immune response to a known antigen. It results from previous contact with the antigen by an immunologically immature individual (fetus or neonate) or by an adult exposed to extreme high-dose or low-dose antigen, or by exposure to radiation, antimetabolites, antilymphocytic serum, etc.
Manifestations of the immune response which are mediated by antigen-sensitized T-lymphocytes via lymphokines or direct cytotoxicity. This takes place in the absence of circulating antibody or where antibody plays a subordinate role.
A single, unpaired primary lymphoid organ situated in the MEDIASTINUM, extending superiorly into the neck to the lower edge of the THYROID GLAND and inferiorly to the fourth costal cartilage. It is necessary for normal development of immunologic function early in life. By puberty, it begins to involute and much of the tissue is replaced by fat.
Endogenous tissue constituents that have the ability to interact with AUTOANTIBODIES and cause an immune response.
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
Nuclear antigens encoded by VIRAL GENES found in HUMAN HERPESVIRUS 4. At least six nuclear antigens have been identified.
A soluble substance elaborated by antigen- or mitogen-stimulated T-LYMPHOCYTES which induces DNA synthesis in naive lymphocytes.
A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.
A cell line derived from cultured tumor cells.
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
A sex-specific cell surface antigen produced by the sex-determining gene of the Y chromosome in mammals. It causes syngeneic grafts from males to females to be rejected and interacts with somatic elements of the embryologic undifferentiated gonad to produce testicular organogenesis.
A cell adhesion molecule of the immunoglobulin superfamily that is expressed in ENDOTHELIAL CELLS and is involved in INTERCELLULAR JUNCTIONS.
Antigens stimulating the formation of, or combining with heterophile antibodies. They are cross-reacting antigens found in phylogenetically unrelated species.
CD4-positive T cells that inhibit immunopathology or autoimmune disease in vivo. They inhibit the immune response by influencing the activity of other cell types. Regulatory T-cells include naturally occurring CD4+CD25+ cells, IL-10 secreting Tr1 cells, and Th3 cells.
Antibodies obtained from a single clone of cells grown in mice or rats.
Antigenic determinants recognized and bound by the T-cell receptor. Epitopes recognized by the T-cell receptor are often located in the inner, unexposed side of the antigen, and become accessible to the T-cell receptors after proteolytic processing of the antigen.
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
A heterodimeric protein that is a cell surface antigen associated with lymphocyte activation. The initial characterization of this protein revealed one identifiable heavy chain (ANTIGENS, CD98 HEAVY CHAIN) and an indeterminate smaller light chain. It is now known that a variety of light chain subunits (ANTIGENS, CD98 LIGHT CHAINS) can dimerize with the heavy chain. Depending upon its light chain composition a diverse array of functions can be found for this protein. Functions include: type L amino acid transport, type y+L amino acid transport and regulation of cellular fusion.
The hepatitis B antigen within the core of the Dane particle, the infectious hepatitis virion.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
The complex formed by the binding of antigen and antibody molecules. The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES.
They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system.
The sum of the weight of all the atoms in a molecule.
Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.
A group of the D-related HLA antigens found to differ from the DR antigens in genetic locus and therefore inheritance. These antigens are polymorphic glycoproteins comprising alpha and beta chains and are found on lymphoid and other cells, often associated with certain diseases.
Immunoglobulins produced in response to VIRAL ANTIGENS.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
A glycolipid, cross-species antigen that induces production of antisheep hemolysin. It is present on the tissue cells of many species but absent in humans. It is found in many infectious agents.
Elements of limited time intervals, contributing to particular results or situations.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
An inhibitory B7 antigen that has specificity for the T-CELL receptor PROGRAMMED CELL DEATH 1 PROTEIN. CD274 antigen provides negative signals that control and inhibit T-cell responses and is found at higher than normal levels on tumor cells, suggesting its potential role in TUMOR IMMUNE EVASION.
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
A species of POLYOMAVIRUS originally isolated from Rhesus monkey kidney tissue. It produces malignancy in human and newborn hamster kidney cell cultures.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
Substances that augment, stimulate, activate, potentiate, or modulate the immune response at either the cellular or humoral level. The classical agents (Freund's adjuvant, BCG, Corynebacterium parvum, et al.) contain bacterial antigens. Some are endogenous (e.g., histamine, interferon, transfer factor, tuftsin, interleukin-1). Their mode of action is either non-specific, resulting in increased immune responsiveness to a wide variety of antigens, or antigen-specific, i.e., affecting a restricted type of immune response to a narrow group of antigens. The therapeutic efficacy of many biological response modifiers is related to their antigen-specific immunoadjuvanticity.
Antigens that exist in alternative (allelic) forms in a single species. When an isoantigen is encountered by species members who lack it, an immune response is induced. Typical isoantigens are the BLOOD GROUP ANTIGENS.
Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell.
A melanosome-associated protein that plays a role in the maturation of the MELANOSOME.
The genetic region which contains the loci of genes which determine the structure of the serologically defined (SD) and lymphocyte-defined (LD) TRANSPLANTATION ANTIGENS, genes which control the structure of the IMMUNE RESPONSE-ASSOCIATED ANTIGENS, HUMAN; the IMMUNE RESPONSE GENES which control the ability of an animal to respond immunologically to antigenic stimuli, and genes which determine the structure and/or level of the first four components of complement.
Bone marrow-derived lymphocytes that possess cytotoxic properties, classically directed against transformed and virus-infected cells. Unlike T CELLS; and B CELLS; NK CELLS are not antigen specific. The cytotoxicity of natural killer cells is determined by the collective signaling of an array of inhibitory and stimulatory CELL SURFACE RECEPTORS. A subset of T-LYMPHOCYTES referred to as NATURAL KILLER T CELLS shares some of the properties of this cell type.
A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
Form of passive immunization where previously sensitized immunologic agents (cells or serum) are transferred to non-immune recipients. When transfer of cells is used as a therapy for the treatment of neoplasms, it is called adoptive immunotherapy (IMMUNOTHERAPY, ADOPTIVE).

Core 2-containing O-glycans on CD43 are preferentially expressed in the memory subset of human CD4 T cells. (1/292)

Human CD4 T cells can be divided into two functionally distinct subsets: a CD45RO+ memory subset and a CD45RA+ naive subset. In an attempt to identify novel cell surface molecules on these cells, we have developed a mAb, anti-1D4. The antigen defined by anti-1D4 was preferentially expressed on the memory subset of freshly isolated peripheral CD4 T cells and 1D4+ CD4 T cells functionally corresponded to memory T cells. Retrovirus-mediated expression cloning revealed that the 1 D4 antigen is human CD43. Transfection of CHO-leu cells, which stably express human CD43, with core 2 beta-1,6-N-acetylglucosaminyltransferase (C2GnT) conferred expression of the 1D4 antigen and mRNA of C2GnT was detected by RT-PCR only in 1D4+ T cells but not in 1D4- T cells, implying that the 1 D4 antigen is composed of core 2-containing O-glycans on CD43. Reactivity with anti-1 D4 was completely abolished when cells were treated with neuraminidase, while them remained weak binding of anti-T305, a previously described mAb which also reacts with CD43 modified with core 2-containing O-glycans. Moreover, anti-1D4 markedly reacted with NIH-3T3 cells expressing human CD43 and low levels of endogenous C2GnT, whereas anti-T305 reacted slightly. These results indicate that the 1D4 antigen is distinct from the epitope defined by anti-T305 and anti-1D4 is a more sensitive probe to detect core 2-containing O-glycans than anti-T305. Taken together, our results indicate that core 2-containing O-glycans, whose expression can easily be detected with anti-1D4, are preferentially expressed in the CD45RO+ memory subset of CD4 T cells.  (+info)

B cell response after MMTV infection: extrafollicular plasmablasts represent the main infected population and can transmit viral infection. (2/292)

The immune response to mouse mammary tumor virus (MMTV) relies on the presentation of an MMTV-encoded superantigen by infected B cells to superantigen-specific T cells. The initial extrafollicular B cell differentiation involved the generation of B cells expressing low levels of B220. These B220low B cells corresponded to plasmablasts that expressed high levels of CD43 and syndecan-1 and were CD62 ligand- and IgD-. Viral DNA was detected nearly exclusively in these B220low B cells by PCR, and retroviral type-A particles were observed in their cytoplasm by electron microscopy. An MMTV transmission to the offspring was also achieved after transfer of B220low CD62 ligand- CD43+ plasmablasts into noninfected females. These data suggest that B220low plasmablasts, representing the bulk of infected B cells, are capable of sustaining viral replication and may be involved in the transmission of MMTV.  (+info)

Probing cell-surface architecture through synthesis: an NMR-determined structural motif for tumor-associated mucins. (3/292)

Cell-surface mucin glycoproteins are altered with the onset of oncogenesis. Knowledge of mucin structure could be used in vaccine strategies that target tumor-associated mucin motifs. Thus far, however, mucins have resisted detailed molecular analysis. Reported herein is the solution conformation of a highly complex segment of the mucin CD43. The elongated secondary structure of the isolated mucin strand approaches the stability of motifs found in folded proteins. The features required for the mucin motif to emerge are also described. Immunocharacterization of related constructs strongly suggests that the observed epitopes represent distinguishing features of tumor cell-surface architecture.  (+info)

Structural requirements for CD43 function. (4/292)

The regulation of T cell activation and adhesion by CD43 (leukosialin, sialophorin) has been thought to be mainly a function of the large size and negative charge of the extracellular domain of the protein. In this work, we demonstrate that the cytoplasmic tail is both necessary and sufficient for the negative regulatory effect of CD43 on cell-cell adhesion. Expression of mutant CD43 proteins in primary T cells from CD43-deficient mice demonstrated that the antiproliferative effect of CD43 is also dependent upon the cytoplasmic tail. In contrast, Ab-mediated costimulation through CD43 does not require the intracellular domain of CD43. These data demonstrate that CD43 primarily serves as a negative regulator of T cell activation and adhesion, and that this is mediated not exclusively by passive effects of the extracellular domain, but requires participation of the cytoplasmic tail, perhaps through interactions with the cytoskeleton, or alternatively, active regulation of intracellular signaling pathways.  (+info)

An antiserum raised against the recombinant cytoplasmic tail of the human CD43 glycoprotein identifies CD43 in many mammalian species. (5/292)

Leukosialin or CD43 is a heavily O-glycosylated transmembrane protein expressed on all cells of the haematopoietic cell lineage with the exception of red blood cells and mature B cells. This antigen has been identified in human, mouse and rat with monoclonal antibodies. Although orthologues of many human and rodent leucocyte cell surface antigens have been described in recent years, CD43, despite its abundance on human and rodent cells, remained uncharacterized in other vertebrate species. The comparison of CD43 amino acid sequences from human, mouse and rat indicated a high level of homology in the cytoplasmic domain. A serum, (p.aCD43cp) raised against the recombinant cytoplasmic tail of the human CD43, was shown not only to recognize human CD43, but it bound to putative CD43 orthologues in many mammalian species. CD43 was found to be expressed in the same leucocyte subpopulations and circumstantial evidence suggested that CD43 is also regulated similarly during leucocyte ontogeny in all species investigated. As CD43+ cells were readily observed in fixed tissues, the p.aCD43cp serum may be used as a reliable reagent for the verification of the haematopoietic origin of infiltrations and, used together with other reagents, for the serological characterization of normal and pathological lymphoid tissues and lymphoid infiltrations in experimental work and in animal disease.  (+info)

Modulation of integrin function in hematopoietic progenitor cells by CD43 engagement: possible involvement of protein tyrosine kinase and phospholipase C-gamma. (6/292)

Attachment of cells to extracellular matrix components is critical for the regulation of hematopoiesis. CD43 is a mucin-like transmembrane sialoglycoprotein expressed on the surface of almost all hematopoietic cells. A highly extended structure of extracellular mucin with negative charge may function as a repulsive barrier to hematopoietic cells. However, some investigators have shown that CD43 has proadhesive properties, and engagement of CD43 has been reported to upregulate integrin-mediated cell adhesion in T cells. We found that cross-linking of CD43 with monoclonal antibodies (MoAbs) enhanced integrin alpha4beta1 (very late antigen [VLA]-4) and alpha5 beta1 (VLA-5)-dependent adhesion of human cord blood CD34(+) cells to fibronectin. CD34(+) CD38(hi), but not CD34(+)CD38(-/low) cells responded significantly to the stimulus, suggesting that committed, but not stem and more immature progenitors are sensitive to CD43-mediated activation of integrin. To elucidate the molecular mechanism leading to integrin activation, we used the growth factor-dependent cell line MO7e. Cross-linking of CD43 induced tyrosine phosphorylation of several intracellular molecules including the protein tyrosine kinase Syk, the proto-oncogene product Cbl, and phospholipase C (PLC)-gamma2 in MO7e cells. Moreover, protein tyrosine kinase inhibitor herbimycin A and PLC inhibitor U73122 both blocked CD43-induced enhancement of adhesion to fibronectin. These results indicate that signals mediated through CD43 may increase integrin affinity to fibronectin via a pathway dependent on protein tyrosine kinase and PLC-gamma activation in hematopoietic progenitors.  (+info)

Characterization of anergic anti-DNA B cells: B cell anergy is a T cell-independent and potentially reversible process. (7/292)

Anti-single stranded DNA (ssDNA) and anti-double stranded DNA (dsDNA) B cells are regulated in non-autoimmune mice. In this report we show that while both anti-ssDNA and anti-dsDNA B cells are blocked in their ability to differentiate into antibody-secreting cells, other phenotypic and functional characteristics distinguish them from one another. Splenic anti-ssDNA B cells are found distributed throughout the B cell follicle, and are phenotypically mature and long-lived. On the other hand, splenic anti-dsDNA B cells are short-lived, exhibit an immature and antigen-experienced phenotype, and localize to the T-B interface of the splenic follicle. Functionally, anti-ssDNA B cells proliferate, albeit suboptimally, in response to anti-IgM, lipopolysaccharide (LPS) and CD40L/IL-4 + anti-IgM stimulation, and tyrosine phosphorylate intracellular proteins upon mIgM cross-linking. Anti-dsDNA B cells, on the other hand, are functionally unresponsive to anti-IgM and LPS stimulation, and do not phosphorylate intracellular proteins, including Syk, upon mIg stimulation. Importantly, anti-DNA B cell anergy is maintained in the absence of T cells since both anti-ssDNA and anti-dsDNA B cells are as efficiently regulated in RAG2(-/-) mice as in their RAG2(+/+) counterparts. Interestingly, the severely anergic state of anti-dsDNA B cells is partially reversible upon stimulation with CD40 ligand and IL-4. In response to these signals, anti-dsDNA B cells remain viable, up-regulate cell surface expression of B7-2 and IgM, and restore their ability to proliferate and phosphorylate Syk upon mIg cross-linking. Collectively, these data suggest that anti-DNA B cell anergy encompasses distinct phenotypes which, even in its most severe form, may be reversible upon stimulation with T cell-derived factors.  (+info)

A common signaling pathway via Syk and Lyn tyrosine kinases generated from capping of the sialomucins CD34 and CD43 in immature hematopoietic cells. (8/292)

The sialomucin CD34 is a useful marker for hematopoietic stem/progenitor cells. However, the role of CD34 remains poorly understood. Here we investigate the functions of CD34 and another sialomucin CD43 coexpressed on hematopoietic stem/progenitor cells. Stimulation of undifferentiated hematopoietic KG1a cells with anti-CD34 or anti-CD43 induced homotypic cytoadhesion, accompanied by formation of a long-lived cap of CD34 and CD43 respectively, which colocalized with F-actin. Stimulation with either antibody specifically increased tyrosine phosphorylation of the identical set of proteins of Lyn, Syk, pp60, pp69, and pp77 at the capping site. These events were similar to those observed in monocytic U937 cells ectopically expressing CD34. After stimulation of KG1a cells, coimmunoprecipitation of Lyn with pp69 and pp77 and of Syk with pp37 was detected in the membrane fraction. Blockade of antibody-induced cap formation by treatment with cytochalasin D leads to inhibition of tyrosine phosphorylation of Syk and pp77 and homotypic cytoadhesion. Moreover, normal human CD34(+) bone marrow cells showed cap formation of CD34 or CD43 after stimulation. These results suggest that crosslinking of either CD34 or CD43 activates the same signaling pathway for cytoadhesion through Lyn, Syk, and the novel tyrosine-phosphorylated proteins within hematopoiesis.  (+info)

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Leukosialin also known as sialophorin or CD43 (cluster of differentiation 43) is a transmembrane cell surface protein that in humans is encoded by the SPN (sialophorin) gene. Sialophorin (leukosialin) is a major sialoglycoprotein on the surface of human T lymphocytes, monocytes, granulocytes, and some B lymphocytes, which appears to be important for immune function and may be part of a physiologic ligand-receptor complex involved in T-cell activation. Defects in the CD43 molecule are associated with the development of Wiskott-Aldrich syndrome. It also appears in about 25% of intestinal MALTomas.[citation needed] Using immunohistochemistry, CD43 can be demonstrated in the paracortical T-cells of healthy lymph nodes and tonsils; it is also positive in a range of lymphoid and myeloid tumours. Although it is present in over 90% of T-cell lymphomas, it is generally less effective at demonstrating this condition than is CD3 antigen. However, it may be useful as part of a panel to demonstrate B-cell ...
Mouse Monoclonal Anti-CD43/Sialophorin Antibody (W3/13) [DyLight 488]. Validated: Flow, IHC-Fr, IHC-P. Tested Reactivity: Rat. 100% Guaranteed.
Promab CD106 - This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different
Promab CD106 - This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different
Supplier: ProMab Technologies Type of Product: Monoclonal Antibody Description: This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may
Adenoidal tissue (also known as nasopharyngeal tonsils) of 58% of humans in the pediatric age group contains immature T-lymphoid cells with the phenotype of thymocytes (TdT+, CD1abc+, cytoplasmic CD3 ...
|strong|Rat anti Human CD235a antibody, clone YTH89.1|/strong| recognizes glycophorin A, a major sialoglycoprotein of the human erythrocyte membrane.
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Definition: D2-40 is a monoclonal antibody recognizing podoplanin , a 40 000 kD O-linked sialoglycoprotein located in lymphatic endothelial (...)
generating the core 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is a precursor for many extended O-glycans in ...
Glycophorins are the major sialoglycoprotein component of erythrocyte cell membranes. They are heavily glycosylated and rich in sialic, making the erythrocytes membranes very hydrophilic. This property enables them to circulate without adhering to other cells or the walls of blood vessels. Glycophorins are classified based on the quantity present in the cell membrane. Glycophorin A is the most abundant. The N-terminal glycosylated segment of glycophorin A, which lies outside the erythrocyte membrane, bears MN blood group receptors. It is encoded by the GYPA gene in humans, and many variants exist due to recombination between the GYPA and GYPB genes. Glycophorin A contains a receptor for binding the malaria parasite Plasmodium falciparum; it also binds several viruses. Glycophorin A is also known as sialoglycoprotein alpha, MN sialoglycoprotein, CD235a antigen, MN, GPA, MNS, GPSAT, PAS-2, CD235a, GPErik, HGpMiV, HGpMiXI, and HGpSta(C).. ...
Glycophorins are the major sialoglycoprotein component of erythrocyte cell membranes. They are heavily glycosylated and rich in sialic, making the erythrocytes membranes very hydrophilic. This property enables them to circulate without adhering to other cells or the walls of blood vessels. Glycophorins are classified based on the quantity present in the cell membrane. Glycophorin A is the most abundant. The N-terminal glycosylated segment of glycophorin A, which lies outside the erythrocyte membrane, bears MN blood group receptors. It is encoded by the GYPA gene in humans, and many variants exist due to recombination between the GYPA and GYPB genes. Glycophorin A contains a receptor for binding the malaria parasite Plasmodium falciparum; it also binds several viruses. Glycophorin A is also known as sialoglycoprotein alpha, MN sialoglycoprotein, CD235a antigen, MN, GPA, MNS, GPSAT, PAS-2, CD235a, GPErik, HGpMiV, HGpMiXI, and HGpSta(C).. ...
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TY - JOUR. T1 - Purification and characterization of a human sialoglycoprotein antigen expressed in immature thymocytes and fetal tissues. AU - Cecco, L.. AU - Bond, H. M.. AU - Bonelli, P.. AU - Tuccillo, F.. AU - Cerra, M.. AU - Tassone, P.. AU - Sorice, R.. AU - Lamberti, A.. AU - Morrone, G.. AU - Venuta, S.. PY - 1998. Y1 - 1998. N2 - The monoclonal antibody UN1 was previously produced in our laboratory on the basis of selective reactivity with human thymocytes and has been classified as unclustered by the 5th and 6th International Work shop and Conference on Human Leukocyte Differentiation Antigens. The antigen recognized by mAb UN1 was found to be expressed on the cell surface of immature human thymocytes, a subpopulation of peripheral T lymphocytes and on several fetal tissues including thymus. The UN1 antigen is purified from childrens thymus by ionexchange and affinity chromatography. Two-dimensional electrophoresis shows that the purified antigen displays microheterogeneity appearing ...
An integral sialoglycoprotein with Mr approximately 130,000 (Sgp 130) and highest expression in adult chicken gizzard smooth muscle has been recently identified as an excellent candidate for classification as a plasma membrane protein natively associated (directly or indirectly) with actin microfilaments (Rogalski, A.A., and S.J. Singer, 1985, J. Cell Biol., 101:785-801). In this study, the relative in situ distributions of the Sgp 130 integral species (a designation that also includes non-smooth muscle molecular forms) and the peripheral protein, vinculin, have been simultaneously revealed for the first time in selected cultured cells and tissues abundant in microfilament-membrane attachment sites, particularly, smooth and cardiac muscle. Specific antibody probes against Sgp 130 (mouse mAb 30B6) and vinculin (affinity-purified rabbit antibody) were used in double indirect immunofluorescent and immunoelectron microscopic experiments. In contrast to the widespread distributions of vinculin at ...
Description: This gene encodes a member of the N-acetylglucosaminyltransferase family. The encoded protein is a beta-6-N-acetylglucosamine-transferase that catalyzes the formation of core 2 and core 4 O-glycans on mucin-type glycoproteins ...
What is Wiskott-Aldrich syndrome? Wiskott-Aldrich syndrome results in greater susceptibility to infections and abnormal bleeding. The latter occurs due to unusually small, dysfunctional platelets. What is the treatment for Wiskott-Aldrich syndrome? This condition is often cured by stem transplantation from bone marrow, peripheral blood, or cord blood.
If the KIR receptors of NK cell find their cognate antigen on the surface of target cell, formation of the lytic synapse is ... d-SMAC (distal-SMAC) enriched in CD43 and CD45 molecules. New investigations, however, have shown that a "bull's eye" is not ... The process of formation begins when the T-cell receptor (TCR) binds to the peptide:MHC complex on the antigen-presenting cell ... Play media In immunology, an immunological synapse (or immune synapse) is the interface between an antigen-presenting cell or ...
Antigen-naive T cells expand and differentiate into memory and effector T cells after they encounter their cognate antigen ... Exhausted T cells typically indicate higher levels of CD43, CD69 and inhibitory receptors combined with lower expression of ... T cell exhaustion can be triggered by several factors like persistent antigen exposure and lack of CD4 T cell help. Antigen ... These self-antigens are expressed by thymic cortical epithelial cells on MHC molecules on the surface of cortical epithelial ...
He also found the novel antigen JL1 expressed in thymocytes (J Exp Med. 178:1447-51, 1993). JL1 is a unique epitope of CD43 ... He also found the novel antigen JL1 on thymocytes, which has been developed as a therapeutic target for leukemia. Today he is ... JL1, a novel differentiation antigen of human cortical thymocyte. J Exp Med. 178:1447-51, 1993 Choi EY, Park WS, Jung KC, Chung ... Another important achievement of his research is the induction of antigen-specific T cell tolerance, which has been a distant ...
... the hematopoietic mucin-like family of molecules that includes leukosialin/CD43 and stem cell antigen CD34; the lysosomal/ ... "MACROPHAGE ANTIGEN CD68; CD68". omim.org. Retrieved 16 September 2017. Leong, Anthony S-Y; Cooper, Kumarason; Leong, F Joel W-M ... Other names or aliases for this gene in humans and other animals include: CD68 Molecule, CD68 Antigen, GP110, Macrosialin, ... "Macrophage/histiocytic antigen CD68 expression in neoplastic and reactive lymph nodes". Roczniki Akademii Medycznej W ...
... antigens, cd43 MeSH D12.776.395.560.631.650.264 - antigens, cd164 The list continues at List of MeSH codes (D12.776) § MeSH ... antigens, cd22 MeSH D12.776.395.550.200.098 - antigens, cd24 MeSH D12.776.395.550.200.131 - antigens, cd31 MeSH D12.776.395.550 ... antigens, cd146 MeSH D12.776.395.550.200.175 - antigens, cd164 MeSH D12.776.395.550.200.200 - cadherins MeSH D12.776.395.550. ... antigens, cd56 MeSH D12.776.395.550.200.250.520.578 - neural cell adhesion molecule l1 MeSH D12.776.395.550.200.275 - integrin ...
... antigens, cd38 MeSH D23.050.301.264.035.140 - antigens, cd40 MeSH D23.050.301.264.035.143 - antigens, cd43 MeSH D23.050.301.264 ... antigens, cd38 MeSH D23.101.100.110.140 - antigens, cd40 MeSH D23.101.100.110.143 - antigens, cd43 MeSH D23.101.100.110.145 - ... antigens, cd15 MeSH D23.101.100.900.131 - antigens, cd31 MeSH D23.101.100.920 - antigens, ly MeSH D23.101.100.930 - antigens, ... forssman antigen MeSH D23.050.285.018 - antigens, cd24 MeSH D23.050.285.025 - antigens, cd30 MeSH D23.050.285.040 - antigens, ...
... antigens, cd43 MeSH D12.644.233.800.350 - glycophorin MeSH D12.644.233.900 - teicoplanin MeSH D12.644.233.925 - vancomycin MeSH ...
... +antigen at the US National Library of Medicine Medical Subject Headings (MeSH) Human SPN genome location and SPN gene ... Defects in the CD43 molecule are associated with the development of Wiskott-Aldrich syndrome. It also appears in about 25% of ... 1998). "CD43 interacts with moesin and ezrin and regulates its redistribution to the uropods of T lymphocytes at the cell-cell ... 2000). "CD43-mediated signals induce DNA binding activity of AP-1, NF-AT, and NFkappa B transcription factors in human T ...
This process prevents the circulation of T cells that are self-reactive and recognise self antigen. Both galectin-1 and ... CD43 and CD45 are involved in apoptosis. Galectin-7 is expressed under the p53 promoter and may have a key role in regulating ...
B1b cells seem to recognize more types of antigens including intracellular antigens. Previously, B1b cell antigen recognition ... Human B1 cells have been found to have marker profile of CD20+CD27+CD43+CD70- and could either be CD5+ or CD5-, which has been ... making antibodies against antigens and acting as antigen-presenting cells. These B1 cells are commonly found in peripheral ... Hence, there appears to be a role for self or foreign antigen in shaping the repertoire of the B-1 B cell compartment. B1 B ...
Antigen-naïve T cells expand and differentiate into memory and effector T cells after they encounter their cognate antigen ... Exhausted T cells typically indicate higher levels of CD43, CD69 and inhibitory receptors combined with lower expression of ... T cell exhaustion can be triggered by several factors like persistent antigen exposure and lack of CD4 T cell help.[57] Antigen ... Antigen discrimination[edit]. A unique feature of T cells is their ability to discriminate between healthy and abnormal (e.g. ...
Hematopoietic progenitor cell antigen CD34 also known as CD34 antigen is a protein that in humans is encoded by the CD34 gene.[ ... Drew E, Merzaban JS, Seo W, Ziltener HJ, McNagny KM (Jan 2005). "CD34 and CD43 inhibit mast cell adhesion and are required for ... A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells". Journal of ... Antigens, CD34 at the US National Library of Medicine Medical Subject Headings (MeSH) ...
Sialyl Lewis X is also one of the most important blood group antigens and is displayed on the terminus of glycolipids that are ... CD43 or CD44. This sialyl-Lewisx mediated binding to selectins allows circulating leukocytes to stick to and roll along ... Sialyl Lewis x is also an inflammation-associated antigen on liver cells. It becomes over expressed on diseased liver cells and ... Sialyl LewisX (sLeX) also known as cluster of differentiation 15s (CD15s) or stage-specific embryonic antigen 1 (SSEA-1), is a ...
Antigens,+CD34 at the US National Library of Medicine Medical Subject Headings (MeSH) Mouse CD Antigen Chart Human CD Antigen ... Drew E, Merzaban JS, Seo W, Ziltener HJ, McNagny KM (Jan 2005). "CD34 and CD43 inhibit mast cell adhesion and are required for ... CD34 derives its name from the cluster of differentiation protocol that identifies cell surface antigens. CD34 was first ... A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells". Journal of ...
4-1BBL (4-1BB ligand) is found on APCs (antigen presenting cells) and binds to 4-1BB. ...
Tissue Antigens (англ.)русск. : journal. - 2007. - Vol. 68, no. 6. - P. 509-517. - DOI:10.1111/j.1399-0039.2006.00726.x. - PMID ...
In humans, the CD44 antigen is encoded by the CD44 gene on Chromosome 11.[5] CD44 has been referred to as HCAM (homing cell ... The CD44 antigen is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. ... Indian blood group system at BGMUT Blood Group Antigen Gene Mutation Database at NCBI, NIH ... "Carcinoembryonic antigen and CD44 variant isoforms cooperate to mediate colon carcinoma cell adhesion to E- and L-selectin in ...
The organization of the SELPLG gene closely resembles that of CD43 and the human platelet glycoprotein GpIb-alpha both of which ... Fuhlbrigge RC, Kieffer JD, Armerding D, Kupper TS (1997). "Cutaneous lymphocyte antigen is a specialized form of PSGL-1 ... Tissue Antigens. 54 (5): 499-507. doi:10.1034/j.1399-0039.1999.540507.x. PMID 10599889. Epperson TK, Patel KD, McEver RP, ...
CD97 antigen je protein koji je kod ljudi kodiran CD97 genom.[1][2][3] ... CD43 • CD44 • CD45 • CD46 • CD47 • CD48 • CD49 (a, b, c, d, e, f) • CD50 ... 2001). „Tissue distribution of the human CD97 EGF-TM7 receptor". Tissue Antigens. 57 (4): 325-31. PMID 11380941. doi:10.1034/j. ... Expression cloning and chromosomal mapping of the leukocyte activation antigen CD97, a new seven-span transmembrane molecule of ...
1997). "The Oka blood group antigen is a marker for the M6 leukocyte activation antigen, the human homolog of OX-47 antigen, ... 1992). "Human leukocyte activation antigen M6, a member of the Ig superfamily, is the species homologue of rat OX-47, mouse ... Kasinrerk W, Fiebiger E, Stefanová I, Baumruker T, Knapp W, Stockinger H (1992). "Human leukocyte activation antigen M6, a ... Ok blood group system at BGMUT Blood Group Antigen Gene Mutation Database at NCBI, NIH ...
CD74 (англ. HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain) - мембранный белок ... II histocompatibility antigen gamma chaingamma chain of class II antigensIiHLA-DR antigens-associated invariant chainIa antigen ... Riberdy J.M., Newcomb J.R., Surman M.J., Barbosa J.A., Cresswell P. HLA-DR molecules from an antigen-processing mutant cell ... Machamer C.E., Cresswell P. Biosynthesis and glycosylation of the invariant chain associated with HLA-DR antigens (англ.) // ...
Seligman P. A., Butler C. D., Massey E. J., etal. The p97 antigen is mapped to the q24-qter region of chromosome 3; the same ... Le Beau M. M., Diaz M. O., Plowman G. D., etal. Chromosomal sublocalization of the human p97 melanoma antigen. (англ.) // Hum. ... Plowman G. D., Brown J. P., Enns C. A., etal. Assignment of the gene for human melanoma-associated antigen p97 to chromosome 3 ... Rose T. M., Plowman G. D., Teplow D. B., etal. Primary structure of the human melanoma-associated antigen p97 ( ...
It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte ... CD43, and ICAM-2". The Journal of Cell Biology. 140 (4): 885-95. doi:10.1083/jcb.140.4.885. PMC 2141743. PMID 9472040. ...
"Regulation of Cbl molecular interactions by the co-receptor molecule CD43 in human T cells". J. Biol. Chem. 276 (1): 729-37. ... of the c-cbl protooncogene is the 120-kDa tyrosine-phosphorylated protein in Jurkat cells activated via the T cell antigen ...
1991). „Expression of the YB5.B8 antigen (c-kit proto-oncogene product) in normal human bone marrow". Blood. 78 (1): 30-7. PMID ... CD43 • CD44 • CD45 • CD46 • CD47 • CD48 • CD49 (a, b, c, d, e, f) • CD50 ... 2003). „Signal transduction-associated and cell activation-linked antigens expressed in human mast cells". Int. J. Hematol. 75 ...
... is a co-receptor of the T cell receptor (TCR) and assists the latter in communicating with antigen-presenting cells. The ... Leucocyte typing: human leucocyte differentiation antigens detected by monoclonal antibodies: specification, classification, ... T cells displaying CD4 molecules (and not CD8) on their surface, therefore, are specific for antigens presented by MHC II and ... CD1+Antigen at the US National Library of Medicine Medical Subject Headings (MeSH) ...
In addition to aiding with cytotoxic T cell antigen interactions the CD8 co-receptor also plays a role in T cell signaling. The ... the CD8 co-receptor plays a role in T cell signaling and aiding with cytotoxic T cell antigen interactions. ... This affinity keeps the T cell receptor of the cytotoxic T cell and the target cell bound closely together during antigen- ... Once the T cell receptor binds its specific antigen Lck phosphorylates the cytoplasmic CD3 and ζ-chains of the TCR complex ...
CD43 • CD44 • CD45 • CD46 • CD47 • CD48 • CD49 (a, b, c, d, e, f) • CD50 ... 1996). "CD88 antibodies specifically bind to C5aR on dermal CD117+ and CD14+ cells and react with a desmosomal antigen in human ...
van Rhenen A., van Dongen G. A., Kelder A., et al. The novel AML stem cell associated antigen CLL-1 aids in discrimination ...
... uveitis antigens induce CXCR3- and CXCR5-expressing lymphocytes and immature dendritic cells to migrate (англ.) // Blood (англ ...
CD43 - CD164 - MUC1 - MUC2 - MUC3A - MUC3B - MUC4 - MUC5AC - MUC5B - MUC6 - MUC7 - MUC8 - MUC12 - MUC13 - MUC15 - MUC16 - MUC17 ... 2000). "Characterization of a new member of the TNF family expressed on antigen presenting cells.". Biol. Chem. 380 (12): 1443- ... CD43 • CD44 • CD45 • CD46 • CD47 • CD48 • CD49 (a, b, c, d, e, f) • CD50 ... CD43, Glikoforin, Glikoforin C) • Trombopoetin • Tiroglobulin • Tiroksin-vezujući proteini • Transkortin • Faktor nekroze ...
I. Partial characterization of soluble Ki-1 antigen and detection of the antigen in cell culture supernatants and in serum by ... Josimovic-Alasevic O, Dürkop H, Schwarting R, Backé E, Stein H, Diamantstein T (Jan 1989). "Ki-1 (CD30) antigen is released by ... CD30+Antigens at the US National Library of Medicine Medical Subject Headings (MeSH) ... results from cDNA cloning and sequence comparison of the CD30 antigen from different sources". Molecular Immunology. 31 (17): ...
Macrophage-1 antigen (CD11b+CD18). *VLA-4 (CD49d+CD29). *Glycoprotein IIb/IIIa (ITGA2B+ITGB3) ...
Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) also known as CD66e (Cluster of Differentiation 66e), is a ... 2001). "Heterogeneous RNA-binding protein M4 is a receptor for carcinoembryonic antigen in Kupffer cells". J. Biol. Chem. 276 ( ... CEACAM5, CD66e, CEA, carcinoembryonic antigen related cell adhesion molecule 5. External IDs. HomoloGene: 128801 GeneCards: ... Oikawa S, Nakazato H, Kosaki G (1987). "Primary structure of human carcinoembryonic antigen (CEA) deduced from cDNA sequence". ...
The protein also carries the Jr(a) antigen, which defines the Junior blood group system.[9] ...
"Interaction of glycogen synthase kinase 3beta with the DF3/MUC1 carcinoma-associated antigen and beta-catenin". Molecular and ...
Ebert LM, McColl SR (2002). "Up-regulation of CCR5 and CCR6 on distinct subpopulations of antigen-activated CD4+ T lymphocytes ... This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may ... dendritic cells induce antitumor immunity when genetically fused with nonimmunogenic tumor antigens". J. Immunol. 167 (11): ...
It is also called Lewis x and SSEA-1 (stage-specific embryonic antigen 1) and represents a marker for murine pluripotent stem ... CD15 Antigen at the US National Library of Medicine Medical Subject Headings (MeSH) ... CD15 (3-fucosyl-N-acetyl-lactosamine) is a cluster of differentiation antigen - an immunologically significant molecule. CD15 ...
A new ligand for human leukocyte antigen class II antigens". The Journal of Experimental Medicine. 176 (2): 327-37. doi:10.1084 ... A new ligand for human leukocyte antigen class II antigens". The Journal of Experimental Medicine. 176 (2): 327-37. doi:10.1084 ... antigen processing and presentation of exogenous peptide antigen via MHC class II. ... antigen binding. • transmembrane signaling receptor activity. • MHC class II protein binding. Cellular component. • membrane. • ...
... antigen je protein koji je kod ljudi kodiran CD97 genom.[1][2][3] ... CD43 • CD44 • CD45 • CD46 • CD47 • CD48 • CD49 (a, b, c, d, e, f) • CD50 ... 2001). "Tissue distribution of the human CD97 EGF-TM7 receptor". Tissue Antigens 57 (4): 325-31. PMID 11380941. doi:10.1034/j. ... "Expression cloning and chromosomal mapping of the leukocyte activation antigen CD97, a new seven-span transmembrane molecule of ...
"Entrez Gene: ITGB3 integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)".. *^ May, K. E.; Villar, J.; Kirtley, S.; ... CD61+Antigens at the US National Library of Medicine Medical Subject Headings (MeSH) ...
Antigen-naïve T cells expand and differentiate into memory and effector T cells after they encounter their cognate antigen ... Exhausted T cells typically indicate higher levels of CD43, CD69 and inhibitory receptors combined with lower expression of ... T cell exhaustion can be triggered by several factors like persistent antigen exposure and lack of CD4 T cell help.[51] Antigen ... Antigen discriminationEdit. A unique feature of T cells is their ability to discriminate between healthy and abnormal (e.g. ...
"Direct association of adenosine deaminase with a T cell activation antigen, CD26". Science. 261 (5120): 466-9. doi:10.1126/ ...
B cells can present antigens to a specialized group of helper T cells called TFH cells. If an activated TFH cell recognizes the ... Roles of T cell-B-cell-activating molecule (5c8 antigen) and CD40 in contact-dependent help". Journal of Immunology. 149 (12): ... It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In ... Grewal, IS; Xu, J; Flavell, RA (7 December 1995). "Impairment of antigen-specific T-cell priming in mice lacking CD40 ligand". ...
antigen processing and presentation of peptide antigen via MHC class I. • antigen processing and presentation of exogenous ... antigen processing and presentation of exogenous peptide antigen via MHC class I. • lipoprotein transport. • negative ... peptide antigen via MHC class I, TAP-dependent. • platelet degranulation. • MyD88-dependent toll-like receptor signaling ...
CD43 - CD164 - MUC1 - MUC2 - MUC3A - MUC3B - MUC4 - MUC5AC - MUC5B - MUC6 - MUC7 - MUC8 - MUC12 - MUC13 - MUC15 - MUC16 - MUC17 ... Ruddle NH, Waksman BH (1968). „Cytotoxicity mediated by soluble antigen and lymphocytes in delayed hypersensitivity. 3. ... CD43, Glikoforin, Glikoforin C) • Trombopoetin • Tiroglobulin • Tiroksin-vezujući proteini • Transkortin • Faktor nekroze ...
Primarily, the VCAM-1 protein is an endothelial ligand for VLA-4 (Very Late Antigen-4 or integrin α4β1) of the β1 subfamily of ...
antigen binding. • virus receptor activity. • protein binding. • transmembrane signaling receptor activity. • identical protein ...
sialyl Lewis X antigen on the surface of leukocytes.. H antigen of the ABO blood compatibility antigens. Other examples of ... molecules such as antibodies (immunoglobulins), which interact directly with antigens.. *molecules of the major ...
T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell. • T cell antigen ... CD43 · CD44 · CD45 · CD46 · CD47 · CD48 · CD49 (a, b, c, d, e, f) · CD50 ...
Signal transduction via leukocyte antigen CD43 (Sialophorin). Feedback regulation by protein kinase C. / Wong, R. C.K.; Remold- ... Signal transduction via leukocyte antigen CD43 (Sialophorin). Feedback regulation by protein kinase C. Journal of Immunology. ... The inhibition of CD43-mediated signaling by PMA was due, in part, to uncoupling of CD43 from the signal-transducing G protein ... The inhibition of CD43-mediated signaling by PMA was due, in part, to uncoupling of CD43 from the signal-transducing G protein ...
CD43, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, and gp115, is a large single chain of type I ... APC/Cy7 anti-mouse CD43 Activation-Associated Glycoform Antibody - ... Antigen References 1. van den Berg TK, et al. 2001. J. Immunol. 166:3637.. 2. Moore T, et al. 1994. J. Immunol. 153:4978.. 3. ... It has been reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesion and anti-adhesion, as well as ...
Shop a large selection of products and learn more about Thermo Scientific Lab Vision CD43/T-Cell Marker Ab-1, Mouse Monoclonal ... Antigen. CD43/T-Cell Marker Ab-1. Classification. Monoclonal. Conjugate. Unconjugated. Format. Prediluted. ... CD43 is involved in activation of T cells, B cells, NK cells, and monocytes Host Species: Mouse. Clone: DF-T1. Isotype: IgG1. ... CD43 (leukosialin, sialophorin, or leukocyte sialoglycoprotein) is a cell surface glycoprotein which is expressed on all ...
Antigens, CD34 * Leukosialin * UN1 sialoglycoprotein, human * Vascular Endothelial Growth Factor Receptor-2 ... CD43). CD43 was detected on all types of emerging clonogenic progenitors before expression of CD45, persisted on ... CD43(-)CD31(-)KDR(-) mesenchymal cells. Furthermore, we demonstrated that the first-appearing CD34(+)CD43(+)CD235a(+)CD41a(+/-) ... CD34(+)CD43(+)CD45(+)Lin(-) cells were largely devoid of VE-cadherin and KDR expression and had a distinct FLT3(high)GATA3(low) ...
Mouse monoclonal CD43 antibody [MT1] validated for WB, IHC and tested in Human. Referenced in 2 publications. Immunogen ... antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response. ... ab89691 at 1/20 dilution staining CD43 in Human mantle cell lymphoma by Immunohistochemistry, Paraffin-embedded tissue. Note ...
Mouse monoclonal CD43 antibody [SPN/839] - BSA and Azide free. Validated in WB, IHC, Flow Cyt, ICC/IF and tested in Human. ... Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (for 30 minutes ... Anti-CD43 antibody [SPN/839] - BSA and Azide free. See all CD43 primary antibodies. ... Protein - Recombinant Human CD43 protein (ab171717) SDS-PAGE, MS Secondary - Goat Anti-Mouse IgG H&L (HRP) (ab205719) WB, IP, ...
CD43 plays a regulatory role in cell adhesion, activation, and survival. ... R2/60 antibody reacts with mouse CD43, expressed by most hematopoietic cells. ... CD43 antibody,CD43 R2/60,CD43 antibody clone R2/60 ,CD43 antibody R2/60,CD43,mouse CD43,anti CD43,anti-mouse CD43,CD43 antib, ... Internal Search Keywords: 60042,mouse antibody CD43,mouse antibody CD43 R2/60,mouse antibody CD43 clone R2/60 ,clone R2/60,R2/ ...
Rat Monoclonal Anti-CD43/Sialophorin Antibody (R2/60) [Alexa Fluor® 488]. Validated: WB, Flow. Tested Reactivity: Mouse. 100% ... Alternate Names for CD43/Sialophorin Antibody (R2/60) [Alexa Fluor® 488]. *CD43 antigen ... Home » CD43/Sialophorin » CD43/Sialophorin Antibodies » CD43/Sialophorin Antibody (R2/60) [Alexa Fluor® 488] ... CD43/Sialophorin Antibody (R2/60) [Alexa Fluor® 488] Summary. Immunogen. The immunogen for this antibody was CD43. ...
Mouse Monoclonal Anti-CD43/Sialophorin Antibody (W3/13) [DyLight 488]. Validated: Flow, IHC-Fr, IHC-P. Tested Reactivity: Rat. ... Alternate Names for CD43/Sialophorin Antibody (W3/13) [DyLight 488]. *CD43 antigen ... Home » CD43/Sialophorin » CD43/Sialophorin Antibodies » CD43/Sialophorin Antibody (W3/13) [DyLight 488] ... Blogs on CD43/Sialophorin. There are no specific blogs for CD43/Sialophorin, but you can read our latest blog posts. ...
2, B220+ CD43− pre-B cells are present in wild-type and in the various single and double IgH tg mice and represent ∼8% of the ... B Cell Antigen Receptor Specificity and Surface Density Together Determine B-1 versus B-2 Cell Development. Kong-Peng Lam, ... To determine the developmental potential of B cells bearing two distinct B cell antigen receptors (BCRs), one favoring B-1 and ... B Cell Antigen Receptor Specificity and Surface Density Together Determine B-1 versus B-2 Cell Development ...
Mouse Monoclonal CD43 antibody for IHC. Order anti-CD43 antibody ABIN6297681. ... Antigen CD43 (SPN) Alternative Name Leukosialin (SPN Antibody Abstract) Background Target Description: It recognizes a cell ... Mouse anti-Human CD43 Antibody [IHC only] Immunogen Stimulated human leukocytes were used as the immunogen for the CD43 ... Product Details anti-CD43 Antibody Handling Application Details Target Details back to top ...
Mouse Monoclonal CD43 antibody for Func, FACS, IHC (p), IP, WB. Published in 6 Pubmed References. Order anti-CD43 antibody ... Antigen CD43 (SPN) Alternative Name CD43 (SPN Antibody Abstract) Background CD43 (leukosialin, sialophorin) is a transmembrane ... CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes ... Product Details anti-CD43 Antibody References Images Handling Application Details Target Details back to top ...
The antigens most commonly used include mumps (1 mg/mL), although availability of this antigen has varied; trichophytin (1:30 ... These molecules include CD3, CD2, CD28, and CD43.. T-cell activity can be directly studied. T lymphocytes express certain ... An assessment of functional antibody production in response to natural antigens or antigens to which the population is commonly ... Another class of stimulators includes antigens. PPD, streptokinase, Candida antigen, and tetanus toxoid all activate ...
Human Cancer Antigen CA19-9 (Gastrointestinal Cancer) Antigen Grade. Known contaminants: 65 Units/ml (, 1%) Centocor CA125 RIA ... Hepatitis B Surface Antigen (HBsAg), ad subtype.. Inactivation: Heat Inactivated at 60°C for 15 hours.. ... Hepatitis B Surface Antigen (HBsAg), ay subtype.. Inactivation: Heat Inactivated at 60°C for 15 hours.. ... Cyfra-21-1 Antigen Cyfra-21-1, Cytokeratin 19 Fragment, Low Cross-reactivity (LCR) Calibrator grade.. Known contaminants:. ...
CD43 and NK-cell antigen CD56; negative for CD3, CD5, CD57 and CD11. Large groups of Epstein-Barr virus-encoded RNA-positive ... CD43, CD5 and cyclin D1).[4,66-69] Addition of CD43 to the CD3/CD20 combination adds great value to the diagnostic combination ... CD15, CD20, CD30, CD43, CD45, ALK, CD57 and EMA. Anaplastic large-cell lymphoma. CD45, EMA, ALK1, CD3, CD45RO, CD4, CD8, CD20, ... CD43 is expressed on normal T cells and macrophages/histiocytes. It is not typically coexpressed on normal B cells, while is ...
CD43 is not expressed on resting conventional peripheral B cells. Thus, resting B cells are easily identified by the lack of ... CD43 expression. Additional information: Clone REA840 displays negligible binding to Fc receptors. - Österreich ... Clone REA840 recognizes the mouse CD43 antigen, a glycosylated protein also known as leukosialin or sialophorin, which is ... Clone REA840 recognizes the mouse CD43 antigen, a glycosylated protein also known as leukosialin or sialophorin, which is ...
CD43 (S7) expression identifies peripheral B cell subsets. J. Immunol. 153: 5503. ... CD1.1 Expression by Mouse Antigen-Presenting Cells and Marginal Zone B Cells. Jessica H. Roark, Se-Ho Park, Jayanthi ... The crystal structure of murine CD1: an MHC-like fold with a large hydrophobic antigen binding groove. Science 277: 339. ... I. Immature peripheral B cells in adults are heat-stable antigenhigh and exhibit unique signaling characteristics. J. Immunol. ...
Signal transduction via leukocyte antigen CD43 (sialophorin). Feedback regulation by protein kinase C. J Immunol. 1990 Feb 15; ... Engagement of the monocyte surface antigen CD14 induces lymphocyte function-associated antigen-1/intercellular adhesion ... T-cell antigen receptor (TCR)-alpha/beta heterodimer formation is a prerequisite for association of CD3-zeta 2 into ... Analysis of antigen uptake and presentation by Epstein-Barr virus-transformed human lymphoblastoid B cells. Eur J Immunol. 1984 ...
CD43, CD45, CD52, CD56, CD68, CD7, CD72, CD9, CD95, CD99 ... ProSpecs CD Antigens include: CD4, CD40, CD10, CD11B, CD14, ...
Other stains such as CD3 (K & L), CD5 (M & N) and CD43 (O & P) as well as κ and λ light changes are negative in the follicles. ... The lymph node processes and presents various antigens to either B or T lymphocytes. Antigenic stimulation can result in ... Other stains such as CD3 (K & L), CD5 (M & N) and CD43 (O & P) as well as κ and λ light changes are negative in the follicles. ... CD15, CD20, CD30, CD43, CD45, ALK, CD57 and EMA. Anaplastic large-cell lymphoma. CD45, EMA, ALK1, CD3, CD45RO, CD4, CD8, CD20, ...
CD43+antigen at the US National Library of Medicine Medical Subject Headings (MeSH) ... Defects in the CD43 molecule are associated with the development of Wiskott-Aldrich syndrome.[4] It also appears in about 25% ... CD43 has been shown to interact with EZR[6] and Moesin.[6][7] ... 1998). "CD43 interacts with moesin and ezrin and regulates its ... Leukosialin also known as sialophorin or CD43 (cluster of differentiation 43) is a transmembrane cell surface protein that in ...
CD43+antigen at the US National Library of Medicine Medical Subject Headings (MeSH) Human SPN genome location and SPN gene ... Defects in the CD43 molecule are associated with the development of Wiskott-Aldrich syndrome. It also appears in about 25% of ... 1998). "CD43 interacts with moesin and ezrin and regulates its redistribution to the uropods of T lymphocytes at the cell-cell ... 2000). "CD43-mediated signals induce DNA binding activity of AP-1, NF-AT, and NFkappa B transcription factors in human T ...
Bottom Line: In agreement with these earlier results, we show by a molecular single cell analysis that 4-7% of CD43(+) B cell ... Antigens, CD*. *B-Lymphocytes/immunology*. *Gene Expression Regulation, Developmental/genetics*. *Immunoglobulin Heavy Chains/ ... Bottom Line: In agreement with these earlier results, we show by a molecular single cell analysis that 4-7% of CD43(+) B cell ... In agreement with these earlier results, we show by a molecular single cell analysis that 4-7% of CD43(+) B cell progenitors in ...
The leukocyte antigen CD43 is expressed in different cell lines of nonhematopoietic origin. ... CD43 Christian X Andersson, Julia Fernandez-Rodriguez, Sirle Laos, Dan Baeckström, Gunnar C. Hansson, Christian Haass Biochem J ... CD43 promotes cell growth and helps to evade FAS-mediated apoptosis in non-hematopoietic cancer cells lacking the tumor ... Recombinant glycoprotein E produced in mammalian cells in large-scale as an antigen for varicella-zoster-virus-serology. ...
A and B) Sera were collected from 10-wk-old WT or Cry DKO mice and analyzed for autoantibodies using an auto-antigen microarray ... F) Peritoneal cavity cells were stained with anti-CD19, anti-B220, anti-CD5, and anti-CD43. Shown in the gates are the ... B cell responses to antigen stimulation in vivo. (A) T-dependent antibody responses to NP-KLH in Cry DKO mice. Sera were ... The sera from Cry DKO mice and Cry chimeras reacted strongly to most glomerular and nuclear antigens, suggesting that loss of ...
Exbio - Research products - Antibodies - CD and related antigens - Anti-Hu CD43 FITC ... CD43 is expressed by platelets and at high levels on the surface of all leukocytes; it is negative on resting B lymphocytes and ... Kadaja-Saarepuu L, Laos S, Jääger K, Viil J, Balikova A, Lõoke M, Hansson GC, Maimets T: CD43 promotes cell growth and helps to ... Mody PD, Cannon JL, Bandukwala HS, Blaine KM, Schilling AB, Swier K, Sperling AI: Signaling through CD43 regulates CD4 T-cell ...
CD43: For subclassifying lymphomas (it lights up most leukocytes). CD45 (LCA, leukocyte commmon antigen): Marker for white ... Recoverable antigens involve cross-linking of a tyrosine and an amino-sidechain (usually arginine). Am. J. Clin. Path. 121: 190 ... PSA (prostate specific antigen): Demonstrates that a metastatic cancer is of prostatic origin. RCC-Ma: Pretty specific for ... antigen: Infectious agent. S-100: Helps with the hard calls. Famously lights up melanomas. Also cartilage, melanocytes, sweat ...
TCR signaling induces selective exclusion of CD43 from the T cell-antigen-presenting cell contact site. J. Immunol. 161: 6459. ... Dependence of T cell antigen recognition on the dimensions of an accessory receptor-ligand complex. J. Exp. Med. 190: 31. ... A comparison of the interaction of Shc and the tyrosine kinase ZAP-70 with the T cell antigen receptor ζ chain tyrosine-based ... Normal T cells express two T cell antigen receptor populations, one of which is linked to the cytoskeleton via ζ chain and ...
Cellular immune responses to the vaccine antigen by FACS [ Time Frame: 63 days ]. CD19, IgD, CD27, CD38, CD24 and CD43 B ... Variation in humoral immune response to the vaccine antigen assessed by ELISA [ Time Frame: 3 months ]. The level (g/l) of the ... To explore the quality of the cellular immune response induced by the vaccine antigen by the quantitation of a large panel of ... Will be assessed by measuring the capability of the specific vaccine antigen plasma IgGs to:. *Cross-react with different ...
Expression of mouse CD43 in the B cell lineage of transgenic mice causes impaired immune responses to T-independent antigens.; ... IL-3 enhances both presentation of exogenous particulate antigen in association with class I major histocompatibility antigen ... A pseudogene homologous to mouse transplantation antigens: transplantation antigens are encoded by eight exons that correlate ... of exogenous antigen by tumor-infiltrating antigen-presenting cells.; Proceedings of the National Academy of Sciences of the ...
  • CD43, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, and gp115, is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. (biolegend.com)
  • CD43 (leukosialin, sialophorin, or leukocyte sialoglycoprotein) is a cell surface glycoprotein which is expressed on all thymocytes and T-cells. (fishersci.com)
  • Here, using hESC differentiation in coculture with OP9 stromal cells, we demonstrate that early progenitors committed to hematopoietic development could be identified by surface expression of leukosialin (CD43). (nih.gov)
  • The antibody MEM-59 recognizes neuraminidase-sensitive epitope on CD43 (Leukosialin), a 95-135 kDa type I transmembrane glycoprotein (mucin-type) which is involved in lymphocyte activation. (antibodies-online.com)
  • CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. (antibodies-online.com)
  • 1990) Protein sequence and gene structure for mouse leukosialin (CD43), a T lymphocyte mucin without introns in the coding sequence. (miltenyibiotec.com)
  • 1990) The nucleotide sequence of Ly 48 (mouse leukosialin, sialophorin): the mouse homolog of CD43. (miltenyibiotec.com)
  • Leukosialin also known as sialophorin or CD43 ( cluster of differentiation 43) is a transmembrane cell surface protein that in humans is encoded by the SPN ( s ialo p hori n ) gene . (wikidoc.org)
  • We have resolved B220+ IgM- B-lineage cells in mouse bone marrow into four fractions based on differential cell surface expression of determinants recognized by S7 (leukosialin, CD43), BP-1, and 30F1 (heat stable antigen). (nih.gov)
  • Stefanova I, Hilgert I, Angelisova P, Kristofova H, Horejsi V: Characterization of a 95 kDa human leucocyte sialoglycoprotein: its identity with CD43, gpL115, leukosialin and sialophorin. (sysmex-flowcytometry.com)
  • Mouse anti Human CD43 antibody, clone DFT-1 recognizes human CD43 also known as leukosialin or sialophorin. (bio-rad-antibodies.com)
  • CD43 was detected on all types of emerging clonogenic progenitors before expression of CD45, persisted on differentiating hematopoietic cells, and reliably separated the hematopoietic CD34(+) population from CD34(+)CD43(-)CD31(+)KDR(+) endothelial and CD34(+)CD43(-)CD31(-)KDR(-) mesenchymal cells. (nih.gov)
  • Furthermore, we demonstrated that the first-appearing CD34(+)CD43(+)CD235a(+)CD41a(+/-)CD45(-) cells represent precommitted erythro-megakaryocytic progenitors. (nih.gov)
  • Multipotent lymphohematopoietic progenitors were generated later as CD34(+)CD43(+)CD41a(-)CD235a(-)CD45(-) cells. (nih.gov)
  • Acquisition of CD45 expression by CD34(+)CD43(+)CD45(-)Lin(-) cells was associated with progressive myeloid commitment and a decrease of B-lymphoid potential. (nih.gov)
  • CD34(+)CD43(+)CD45(+)Lin(-) cells were largely devoid of VE-cadherin and KDR expression and had a distinct FLT3(high)GATA3(low)RUNX1(low)PU1(high)MPO(high)IL7RA(high) gene expression profile. (nih.gov)
  • These first steps lead to a series of cytoskeletal reorganizations, including development of lamellopodia and molecular segregation with displacement of large glycoproteins, including CD43 and CD45, from the central area of TCR/MHC contact ( 2 , 3 ). (jimmunol.org)
  • Large, abundant glycoproteins like CD43 and CD45 impose a steric barrier to the interaction of TCR and MHC ( 3 ). (sciencemag.org)
  • The tumoral cells were immunoreactive for leukocyte common antigen (LCA) CD45 (Fig 2B) and for the B-cell markers L26 (CD20) and 4KB5. (ajnr.org)
  • The major O-glycan carrier proteins CD43 and CD162 and isoforms of CD45 expressed on Jurkat cells were precipitated by anti- Tn mAbs with different affinities. (nih.gov)
  • Carbohydrate-independent epitopes of CD43 and CD45 were detected by using MEM-59 and LT45 mAbs. (nih.gov)
  • d-SMAC (distal-SMAC) enriched in CD43 and CD45 molecules. (wikipedia.org)
  • These cell surface antigens can comprise up to 10% of the membrane CD45 proteins. (thefreedictionary.com)
  • This antibody reacts with all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 KDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets. (abnova.com)
  • Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). (abnova.com)
  • Splenocytes of C57BL/6 mice were stained with CD43 antibodies or with the corresponding REA Control antibodies (left image) as well as with CD19 antibodies. (miltenyibiotec.com)
  • However, it may be useful as part of a panel to demonstrate B-cell lymphoblastic lymphoma , since the malignant cells in this condition are often CD43 positive, and may be difficult to stain with other antibodies. (wikidoc.org)
  • Importantly, systemic immunization by the post-fusion HA antigen results in GC B cells targeting the occluded epitope, and induces a class of protective antibodies that have cross-group specificity and afford protection independent of virus neutralization activity. (nature.com)
  • By screening a phage-displayed random peptide library, they also identified the phagotope 2/165 as a mimotope of the UN1 antigen, which was able to elicit specific antibodies against the UN1/CD43 epitope in mice. (aacrjournals.org)
  • These findings support the feasibility of using monoclonal antibodies to identify cancer-associated mimotopes and indicate the potential of cancer-associated CD43 as a target for immunotherapy. (aacrjournals.org)
  • CD antigens for cluster of differentiation, which indicates a defined subset of cellular surface receptors (epitopes) that identify cell type and stage of differentiation, and which are recognized by antibodies. (sinobiological.com)
  • 5] The RS cells in nodular lymphocyte predominance Hodgkin disease (NLPHD) variant stain positive with antibodies to leukocyte common antigen , L26, and pan-B-cell markers in contrast to the RS cells of other variants, which are Leu-M1 positive and nonreactive with pan-B-cell markers. (thefreedictionary.com)
  • Trypanosome clearance from the bloodstream is mediated by antibodies specific for their Variant Surface Glycoprotein (VSG) coat antigens. (prolekare.cz)
  • Monoclonal antibodies against human leucocyte antigens. (abnova.com)
  • We found that cross-linking of CD43 with monoclonal antibodies (MoAbs) enhanced integrin 4β1 (very late antigen [VLA]-4) and 5 β1 (VLA-5)-dependent adhesion of human cord blood CD34 + cells to fibronectin. (ashpublications.org)
  • CD43 has a highly conserved cytoplasmic domain across species 10 and cross-linking of CD43 with specific monoclonal antibodies (MoAbs) has various effects depending on cell types. (ashpublications.org)
  • The distinct V H repertoire that is found in B-1 cells has led to the hypothesis that the specificity of the B cell antigen receptor (BCR) 1 may in fact determine the differentiation of B cells into this subset ( 5 )( 6 ). (rupress.org)
  • These antigens include CD69, IL-2 receptor (CD25), transferring receptors (CD71), and major histocompatibility complex class II molecules (human leukocyte antigen DR). (medscape.com)
  • 2001) Cutting Edge: CD43 functions as a T cell counterreceptor for the macrophage adhesion receptor Sialoadhesin (Siglec-1). (miltenyibiotec.com)
  • CD43 is a murine T cell costimulatory receptor that functions independently of CD28. (labome.org)
  • Together, these data demonstrate that CD43 can serve as a receptor on T cells that can provide CD28-independent costimulation. (labome.org)
  • Tcell antigen receptor (TCR) ligation initiates tyrosine kinase activation, signaling complex assembly, and immune synapse formation. (rupress.org)
  • However, if T cells are exposed to foreign antigens during homeostatic proliferation, a dramatic expansion of antigen-specific T cells was observed in thymic-deficient mice after bone marrow transplantation and adoptive transfer of a small number of antigen-specific T-cell receptor (TCR) transgenic T cells ( 3 ). (aacrjournals.org)
  • The process of formation begins when the T-cell receptor (TCR) binds to the peptide:MHC complex on the antigen-presenting cell and initiates signaling activation through formation of microclusters/lipid rafts. (wikipedia.org)
  • This early, germline cross-reactivity is associated with B cell antigen receptor (BCR)-mediated recognition of variable epitopes with low affinity and supported by the intriguing findings of McGargill's group showing that limiting the generation of highly specific IgG B cells by rapamycin treatment enhanced the breadth of protective antibody responses to influenza 6 . (nature.com)
  • In addition, markers recently developed for PDCs, including CD123 (the interleukin-3 receptor), blood dendritic cell antigen 2 (BDCA2/CD303), (5) and T-cell leukemia/lymphoma 1 (TCL1) and CD2-associated protein (CD2AP), (6) were rarely tested in the literature published prior to 2008. (thefreelibrary.com)
  • This process, known as V(D)J recombination, assembles the vastly diverse antigen receptor genes from numerous V, D, and J coding segments. (bloodjournal.org)
  • This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. (mybiosource.com)
  • Loss of MKK7 in thymocytes and mature B cells results in hyperproliferation in response to growth factor and antigen receptor stimulation and increased thymic cellularity. (pubmedcentralcanada.ca)
  • Thus, MKK7 is an essential and specific regulator of stress-induced SAPK/JNK activation in mast cells and MKK7 negatively regulates growth factor and antigen receptor-driven proliferation in hematopoietic cells. (pubmedcentralcanada.ca)
  • This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. (abnova.com)
  • It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. (abnova.com)
  • In this study, we used immunohistochemical methods to determine whether isolated adult human skin mast cells (SMC) express surface antigens, such as adhesion molecules of the integrin, a variety of leukocyte antigens, and newly identified high affinity IgE receptor. (nii.ac.jp)
  • By interaction with actin-binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. (antibodies-online.com)
  • Alvarado M, Klassen C, Cerny J, Horejsi V, Schmidt RE: MEM-59 monoclonal antibody detects a CD43 epitope involved in lymphocyte activation. (exbio.cz)
  • The specialized junction between a T lymphocyte and an antigen-presenting cell, the immunological synapse, consists of a central cluster of T cell receptors surrounded by a ring of adhesion molecules. (sciencemag.org)
  • Play media In immunology, an immunological synapse (or immune synapse) is the interface between an antigen-presenting cell or target cell and a lymphocyte such as a T/B cell or Natural Killer cell. (wikipedia.org)
  • p-SMAC (peripheral-SMAC) within which the lymphocyte function-associated antigen-1 (LFA-1) and the cytoskeletal protein talin are clustered. (wikipedia.org)
  • This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. (cancerindex.org)
  • The nuclear feature is closer to a lymphocyte, and the cells show lymphoid antigens, such as leukocyte common antigen , CD20, and CD79. (thefreedictionary.com)
  • CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. (antibodies-online.com)
  • To determine the developmental potential of B cells bearing two distinct B cell antigen receptors (BCRs), one favoring B-1 and the other favoring B-2 cell development, we crossed V H 12 insertion mice with mice bearing either V H B1-8 or V H glD42. (rupress.org)
  • This is mediated by the interaction of T cell antigen receptors (TCRs) with their ligands, major histocompatibility molecule-peptide complexes (MHC-peptide) ( 1 ). (sciencemag.org)
  • If the KIR receptors of NK cell find their cognate antigen on the surface of target cell, formation of the lytic synapse is inhibited. (wikipedia.org)
  • To generate antigen receptors, lymphocytes also undergo V(D)J recombination by specific DNA double-strand cleavage, which may be coupled to cell cycle checkpoint pathways to minimize genomic instability (reviewed in refs. (pnas.org)
  • The immense diversity of genes encoding the variable regions of antigen receptors is generated through rearrangement of component V, D, and J segments via a cut-and-paste mechanism known as V(D)J recombination. (bloodjournal.org)
  • The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. (mybiosource.com)
  • Non peptide antigen presentation to T-cell receptors on NKT cells. (sinobiological.com)
  • Ensure accurate, reproducible reults in immunohistochemistry and flow cytometry applications with Thermo Scientific CD43/T-Cell Marker Ab-1, Mouse Monoclonal Antibody. (fishersci.com)
  • The UN1 monoclonal antibody (mAb) recognized CD43 glycoforms expressed in lymphoblastoid T-cell lines and solid tumors, and not in their normal counterpart. (aacrjournals.org)
  • When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. (antibodies-online.com)
  • Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma. (antibodies-online.com)
  • CD43 is a constitutively phosphorylated 115-kDa sialoglycoprotein expressed on a variety of blood cells including lymphocytes and monocytes. (elsevier.com)
  • The 115 kD glycoform of CD43 is expressed on most hematopietic cells including T lymphocytes, NK cells, monocytes, granulocytes, platelets, and CD5 + B cells. (biolegend.com)
  • peripheral blood lymphocytes of various species using anti-human CD43 (MEM-59). (antibodies-online.com)
  • CD43 is expressed by platelets and at high levels on the surface of all leukocytes, it is negative on resting B lymphocytes and erythrocytes. (antibodies-online.com)
  • PPD, streptokinase, Candida antigen, and tetanus toxoid all activate lymphocytes, if the patient has had a prior exposure to the antigen or superantigen. (medscape.com)
  • T lymphocytes express certain antigens after activation. (medscape.com)
  • The lymph node processes and presents various antigens to either B or T lymphocytes. (medscape.com)
  • An alternative mode of CD43 signal transduction activates pro-survival pathways of T lymphocytes Immunology, 150, 87-99. (unam.mx)
  • Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. (mybiosource.com)
  • Staining of human peripheral blood lymphocytes with FITC conjugated Mouse anti Human CD43 antibody, clone DFT-1 ( MCA555F ). (bio-rad-antibodies.com)
  • CD43 is a 381 amino acid single pass type I transmembrane glycoprotein present on the cell surface of multiple cell types including thymocytes, T-lymphocytes, neutrophils, plasma cells and platelets. (bio-rad-antibodies.com)
  • The R2/60 antibody reacts with mouse CD43, a type I transmembrane sialoglycoprotein expressed on the surface of most hematopoietic cells including thymocytes, T cells, B cells, NK cells, monocytes, granulocytes, platelets, and hematopoietic progenitor cells. (stemcell.com)
  • CD43 is a mucin-like transmembrane sialoglycoprotein expressed on the surface of almost all hematopoietic cells. (ashpublications.org)
  • The inhibition of CD43-mediated signaling by PMA was due, in part, to uncoupling of CD43 from the signal-transducing G protein. (elsevier.com)
  • Recombinant full length protein corresponding to Human CD43 aa 1-400. (abcam.com)
  • Similarly, an evaluation of the antibody response after active immunization with polysaccharide or protein antigens is possible. (medscape.com)
  • 0.05) and tumour cell expression for Ki-67 antigen and p53 protein. (bmj.com)
  • Expression of CD43 causes induction of functionally active tumor suppressor p53 protein, but in case of p53 and ARF defficiency CD43 promotes tumour proliferation and viability. (sysmex-flowcytometry.com)
  • Cross-linking of CD43 induced tyrosine phosphorylation of several intracellular molecules including the protein tyrosine kinase Syk, the proto-oncogene product Cbl, and phospholipase C (PLC)-γ2 in MO7e cells. (ashpublications.org)
  • Moreover, protein tyrosine kinase inhibitor herbimycin A and PLC inhibitor U73122 both blocked CD43-induced enhancement of adhesion to fibronectin. (ashpublications.org)
  • These results indicate that signals mediated through CD43 may increase integrin affinity to fibronectin via a pathway dependent on protein tyrosine kinase and PLC-γ activation in hematopoietic progenitors. (ashpublications.org)
  • Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD43 Antibody, Clone R2/60, Biotin, followed by streptavidin (SAV) APC (filled histogram), or a biotin-conjugated rat IgM isotype control antibody, followed by SAV APC (solid line histogram). (stemcell.com)
  • In summary, our data suggest that Tn antigen-Ab binding capacity is determined by the peptide context of the Tn antigen, antigenic specificity of the Ab and class of the immunoglobulin. (nih.gov)
  • The newly generated anti- Tn IgG mAbs with the strong specificity to glycoprotein CD43 can be particularly interesting for the application in leukemia diagnostics and therapy. (nih.gov)
  • Correlated expression of surface IgM (sIgM), CD23, and AA4.1 antigen has also been used to define three nonproliferative subpopulations of immature/transitional peripheral B cells designated: T1 (AA4.1+/CD23-/sIgMhi), T2 (AA4.1+/CD23+/sIgMhi), and T3 (AA4.1+/CD23+/sIgMlo). (thermofisher.com)
  • CD43 (clone MT1) staining of paraffin embedded Human mantle cell lymphoma. (neuromics.com)
  • Staining of human peripheral blood granulocytes with biotinylated Mouse anti Human CD43 antibody, clone DFT-1( MCA555B ). (bio-rad-antibodies.com)
  • Immunoperoxidase staining of human tonsil cryosection with Mouse anti Human CD43 antibody, clone DFT-1 ( MCA555 ) followed by the HISTAR detection system ( STAR3000 ). (bio-rad-antibodies.com)
  • Imunofluorescence staining of human tonsil cryosection with Mouse anti Human CD43, clone DFT-1 ( MCA555 ), red in A and Mouse anti Human CD21 ( MCA1195 ), green in B. C is the merged image with nuclei counterstained blue using DAPI. (bio-rad-antibodies.com)
  • Kadaja L, Laos S, Maimets T: Overexpression of leukocyte marker CD43 causes activation of the tumor suppressor proteins p53 and ARF. (exbio.cz)
  • Kadaja-Saarepuu L, Laos S, Jääger K, Viil J, Balikova A, Lõoke M, Hansson GC, Maimets T: CD43 promotes cell growth and helps to evade FAS-mediated apoptosis in non-hematopoietic cancer cells lacking the tumor suppressors p53 or ARF. (exbio.cz)
  • Results with T-cell markers CD3p and CD43 were negative in the tumor cells. (ajnr.org)
  • One of the most promising approaches to immunotherapy for cancer is based on the recognition of tumor antigens by T cells. (aacrjournals.org)
  • The identification of a large number of well-defined tumor-associated antigens offers an unprecedented opportunity for cancer immunotherapy. (aacrjournals.org)
  • The primary obstacle to the success of active-specific immunotherapy has been the inability of the current vaccines to elicit a strong and persistent immune response to tumor antigens, which for most tumors, particularly melanoma, are primarily self-antigens ( 2 ). (aacrjournals.org)
  • The therapeutic benefit of homeostatic proliferation-induced expansion of T cells against tumor antigens was subsequently shown in an animal tumor model ( 4 ). (aacrjournals.org)
  • We and others have shown that tumor rejection was enhanced by presentation of tumor antigens during homeostatic proliferation in lymphodepleted mice ( 5 - 8 ). (aacrjournals.org)
  • Using a melanoma antigen gp100-specific TCR transgenic T-cell transfer model ( 15 ), our current study is aimed to determine whether vaccination during lymphopenia would drive a rapid and preferential expansion of naive tumor-specific CD8 + T cells and whether the lymphopenic conditions would allow a large fraction of the activated T cells to survive, persist, and mediate tumor regression. (aacrjournals.org)
  • CD antigens are used widely for research, immunotherary, tumor and drug target. (sinobiological.com)
  • Lymphoepithelioma-like carcinoma tumor cells are immunoreactive to cytokeratin and epithelial membrane antigen and are negative for leukocyte common antigen , confirming their epithelial nature. (thefreedictionary.com)
  • The tumor did not stain for leukocyte common antigen , showed weak cytoplasmic positivity for vimentin and epithelial membrane antigen, and displayed strong positivity for AE1-AE3, CAM 5. (thefreedictionary.com)
  • Rat CD43 cell surface antigen. (novusbio.com)
  • Stefanova I, Hilgert I, Kristofova H, Brown R, Low MG, Horejsi V: Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol. (exbio.cz)
  • 66224-1-Ig targets CD43 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples. (ptglab.com)
  • 1992). "Amino acid sequence of human plasma galactoglycoprotein: identity with the extracellular region of CD43 (sialophorin)" . (wikidoc.org)
  • Target Description: It recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43 (Workshop III). (antibodies-online.com)
  • Flow cytometry analysis (surface staining) of human peripheral blood with anti-CD43 (MEM-59) FITC. (exbio.cz)
  • Staurosporine, a potent inhibitor of PKC, abrogated the hyperphosphorylation of CD43 and normalized CD43-mediated signaling in PMA-treated cells. (elsevier.com)
  • CD43 plays dual roles in cell adhesion and anti-adhesion, as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors. (biolegend.com)
  • CD43 is not expressed on resting conventional peripheral B cells. (miltenyibiotec.com)
  • Thus, resting B cells are easily identified by the lack of CD43 expression. (miltenyibiotec.com)
  • Mast cell-derived IL-13 downregulates IL-12 production by skin dendritic cells to inhibit the TH1 cell response to cutaneous antigen exposure. (childrenshospital.org)
  • Cermak L, Simova S, Pintzas A, Horejsi V, Andera L: Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. (exbio.cz)
  • The current case is unique in its extremely late onset of 17 years and in that two distinct components of lymphoma with different morphology and immunophenotypes coexist in the same isolated location with the conventional diffuse large B-cell lymphoma portion being EBV negative while the anaplastic looking component which expresses T-cells antigens is EBV positive. (hindawi.com)
  • Human papillomavirus-like particles mediate functional delivery of plasmid DNA to antigen presenting cells in vivo. (rochester.edu)
  • Lastly, the movement of T cells works against sustained recognition of antigen ( 7 ). (sciencemag.org)
  • To further investigate the nature and complexity of the Tn antigen, we generated seven different anti- Tn mAbs of IgM and IgG classes raised against human Jurkat T cells , which are Tn -positive due to the low activity of T-synthase and mutation in specific chaperone Cosmc. (nih.gov)
  • Various imaging studies have established that T cells that engage antigen-presenting cells (APCs) bearing stimulatory MHC-peptide complexes undergo macromolecular rearrangements that result in the formation of an immune synapse. (rupress.org)
  • Active-specific immunotherapy with dendritic cells loaded with peptide derived from the melanoma antigen, gp100, failed to mediate regression of established B16F10 melanoma in normal mice. (aacrjournals.org)
  • When naive T cells are transferred into lymphodepleted mice (rendered lymphopenic by genetic manipulation, sublethal irradiation, or chemotherapy), they undergo "lymphopenia-driven homeostatic proliferation" in the absence of foreign antigens or inflammatory signals. (aacrjournals.org)
  • Jurkat cells were subjected to SDS PAGE followed by western blot with 66224-1-Ig (CD43 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. (ptglab.com)
  • Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using 66224-1-Ig (CD43 antibody) at dilution of 1:400 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). (ptglab.com)
  • 1X10^6 Jurkat cells were stained with 0.20ug CD43 antibody (66224-1-Ig, red) and control antibody (blue). (ptglab.com)
  • The CD43 antigen is expressed on the membrane and in the cytoplasm of T cells and cells of myeloid lineage. (neuromics.com)
  • Cells expressing the CD43 antigen are reported to include normal and neoplastic T cells. (neuromics.com)
  • To identify the intracellular signals which increase the adhesiveness of leukocyte function-associated antigen 1 (LFA-1), we established an assay system for activation-dependent adhesion through LFA-1/intercellular adhesion molecule 1 ICAM-1 using mouse lymphoid cells reconstituted with human LFA-1 and then introduced constitutively active forms of signaling molecules. (asm.org)
  • The proportion of CD8(+)CD44(+)CD43(+) Gr-1(+) effector memory cells increased after FTY720-treatment, while that of CD8(+)CD44(+)CD62L(+) central memory cells was unchanged. (nih.gov)
  • Despite the conventional association of CD40L expression with CD4 + T cells, other data suggest that CD8 + T cells are also capable of expressing CD40L ( 8 , 12 - 15 ) and this may confer on CD8 + T cells the ability to regulate antigen-specific immune responses. (frontiersin.org)
  • We have previously demonstrated that CD40-CD40L interaction is necessary for activated CD8 + T cells to prime DCs for IL-12 production in an antigen-specific manner ( 13 , 17 ). (frontiersin.org)
  • Antibody to Forssman antigen is usually recognized by agglutination of sheep red blood cells. (thefreedictionary.com)
  • Further analysis showed that metronomic CTX treatment did not delete cells with low expression of CD43, a "memory" phenotype, and that these cells maintained potent restimulatory capacity. (aacrjournals.org)
  • CD43 signals prepare human T cells to receive cytokine differentiation signals. (uniprot.org)
  • CD43 cytoplasmic tail: Protects cells from apoptotic signals, promoting cell survival. (uniprot.org)
  • The CD antigens are protocol used for the identification and investigation of cell surface molecules providing targets for immunophenotyping of cells. (sinobiological.com)
  • 4) In our case, we observed membranous positivity for leukocyte common antigen in the histiocytes that infiltrated the salivary gland, which in our opinion can be regarded as additional evidence that these cells originate from bone marrow-derived monocytes or macrophages. (thefreedictionary.com)
  • In situ staining demonstrated virus capsid antigen in koilocytotic cells and surrounding cells in the hyperplastic epithelial layer. (iospress.com)
  • In combination with other markers of hematopoietic progenitor cells such as Thy-1, Sca-1, c-Kit, CD43, and CD24, the bone marrow Lymphoid-Committed Progenitors (CLP) can be segregated into more primitive and more differentiated subsets based on expression of AA4.1. (thermofisher.com)
  • Cermák L, Símová S, Pintzas A, Horejsí V, Andera L: Molecular mechanisms involved in CD43‑mediated apoptosis of TF‑1 cells: Roles of transcription Daxx expression, and adhesion molecules. (sysmex-flowcytometry.com)
  • However, some investigators have shown that CD43 has proadhesive properties, and engagement of CD43 has been reported to upregulate integrin-mediated cell adhesion in T cells. (ashpublications.org)
  • CD34 + CD38 hi , but not CD34 + CD38 −/low cells responded significantly to the stimulus, suggesting that committed, but not stem and more immature progenitors are sensitive to CD43-mediated activation of integrin. (ashpublications.org)
  • 1-3 Pluripotent hematopoietic stem cells have been reported to express CD43 molecule. (ashpublications.org)
  • In agreement with these earlier results, we show by a molecular single cell analysis that 4-7% of CD43(+) B cell progenitors in wild-type mice rearrange immunoglobulin (Ig)kappa genes before the assembly of a productive VHDHJH joint. (nih.gov)
  • The unglycosylated form of CD43 migrates on SDS-PAGE with a molecular weight of 54 kDa (PMID: 2943740), whereas CD43 from different haematopoietic cell lines displays increased molecular weights since it is glycosylated with O-linked chains that differ in core structure and sialylation. (ptglab.com)
  • ab89691 at 1/20 dilution staining CD43 in Human mantle cell lymphoma by Immunohistochemistry, Paraffin-embedded tissue. (abcam.com)
  • Is there a role for antigen selection in mantle cell lymphoma? (semanticscholar.org)
  • Signal transduction via leukocyte antigen CD43 (Sialophorin). (elsevier.com)
  • Fingerprint Dive into the research topics of 'Signal transduction via leukocyte antigen CD43 (Sialophorin). (elsevier.com)
  • Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (abcam.com)
  • Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). (ptglab.com)
  • IgM mAbs bound the terminal GalNAc residue of the Tn antigen irrespective of the peptide context or with low selectivity to the glycoproteins. (nih.gov)
  • Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:100 (under 10x lens. (ptglab.com)
  • Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:100 (under 40x lens. (ptglab.com)
  • Third, the number of antigenic complexes on the antigen-presenting cell (APC) can be very low ( 6 ). (sciencemag.org)
  • Despite the chemical simplicity of the Tn antigen, its antigenic structure is considered to be complex and the clear determinants of Tn antigenicity remain poorly understood. (nih.gov)
  • 1. one that combines with antibody produced in response to a different but related antigen, owing to similarity of antigenic determinants. (thefreedictionary.com)
  • The immunogen for this antibody was CD43. (novusbio.com)
  • Stimulated human leukocytes were used as the immunogen for the CD43 antibody. (antibodies-online.com)
  • Each antibody is crafted with care according to rigorous protocols for immunogen design and preparation, presentation to host animal, and high-affinity purification against the antigen. (abgent.com)
  • PMA treatment did not affect the surface expression of CD43. (elsevier.com)
  • 5-7 Altered expression of CD43 has been reported in Wiskott-Aldrich syndrome 8 and human immunodeficiency virus (HIV) infection. (ashpublications.org)
  • During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response. (abcam.com)
  • However, because the nonresponse rate to hepatitis B is so high, especially among persons older than 40 years, these antigens remain unreliable in the testing of immune competence. (medscape.com)
  • The SMZ appears especially well equipped for rapid humoral immune responses to blood-borne antigens. (springer.com)
  • However, because innate recognition of disease-specific antigens is a prerequisite for adaptive immune responses to occur, we begin by briefly introducing atherosclerosis-relevant antigens recognized by innate immunity. (jci.org)
  • Although IHC is not commonly necessary in differentiation, epithelial membrane antigen (EMA) highlights most mature sebocytes in sebaceoma, whereas its expression is uncommon in BCC. (thefreelibrary.com)
  • In conclusion, this study describes an amplifying loop in polymorphonuclear neutrophil activation process, whereby ANCA are involved in the membrane expression of their own antigen during cell adhesion. (asnjournals.org)
  • Significantly, in the absence of PMA, staurosporine enhanced the rise in [Ca 2+ ]i triggered by L10, suggesting that engagement of CD43 by activating ligands results in feedback inhibition by PKC. (elsevier.com)
  • CD43 binds ligands such as CD54 and CD169 (siglec-1) and plays regulatory roles in cell adhesion, activation, and survival. (stemcell.com)
  • CD antigens can act in lot of ways, like as recepters or ligands in terms of physiology. (sinobiological.com)
  • Defects in the CD43 molecule are associated with the development of Wiskott-Aldrich syndrome . (wikidoc.org)
  • Today, the HLDA Workshop meeting has been held 10 times and has over 371 CD antigens molecule have been identified. (sinobiological.com)
  • Antigen retrieval was performed for the detection of all antigens by pressure cooking sections in citric acid buffer. (lymphedemapeople.com)
  • Expression of MUC-1 and ALK was determined immunohistochemically after heat-induced antigen retrieval. (aacrjournals.org)
  • In contrast, IgG mAbs recognized the Tn antigen in the context of a specific peptide motif. (nih.gov)
  • The CD antigens / Cluster of differentiation nomenclature was established in the 1st International Workshop and Conference on Human Leukocyte Differentiation Antigens (HLDA), which was held in Paris in 1982. (sinobiological.com)
  • Recombinant Hepatitis B Virus Core Antigen. (origene.com)
  • There are currently no images for CD43/Sialophorin Antibody (NBP1-43413AF488). (novusbio.com)
  • There are currently no images for CD43/Sialophorin Antibody (NB100-64992G). (novusbio.com)
  • Expression cloning of the R2/60 antigen identified the ligand as murine CD43. (labome.org)
  • LFA-1 is not constitutively adhesive, and upregulation of the adhesive activity (avidity) of LFA-1 by external stimuli such as cytokines, chemokines, or antigens is a prerequisite for ligand binding ( 34 , 58 ). (asm.org)
  • In mouse mutants incapable of expressing mu chains, VkappaJkappa joints are detected in the CD43(+) B cell progenitors. (nih.gov)
  • Together with the results obtained in the mouse mutants, these data fit a model in which CD43(+) progenitors rearrange IgH and Igkappa loci independently, with a seven times higher frequency in the former. (nih.gov)
  • It has been reported that CD43 binds to CD54 and Siglec-1. (biolegend.com)
  • Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD43 (Commercially tested by LifeSpan BioSciences. (antibodies-online.com)
  • Polymorphism of the lymphoid background and immunohistochemical stains using keratin and leukocyte common antigen will help make the diagnosis. (thefreedictionary.com)
  • Structure of the human sialophorin (CD43) gene. (wikidoc.org)
  • The 1B11 antibody reacts with the activation-associated glycoform of CD43. (biolegend.com)