Substances that are recognized by the immune system and induce an immune reaction.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
Differentiation antigens found on thymocytes and on cytotoxic and suppressor T-lymphocytes. CD8 antigens are members of the immunoglobulin supergene family and are associative recognition elements in MHC (Major Histocompatibility Complex) Class I-restricted interactions.
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
Substances elaborated by bacteria that have antigenic activity.
A bifunctional enzyme that catalyzes the synthesis and HYDROLYSIS of CYCLIC ADP-RIBOSE (cADPR) from NAD+ to ADP-RIBOSE. It is a cell surface molecule which is predominantly expressed on LYMPHOID CELLS and MYELOID CELLS.
Glycoproteins found on immature hematopoietic cells and endothelial cells. They are the only molecules to date whose expression within the blood system is restricted to a small number of progenitor cells in the bone marrow.
Differentiation antigens expressed on B-lymphocytes and B-cell precursors. They are involved in regulation of B-cell proliferation.
A member of the tumor necrosis factor receptor superfamily with specificity for CD40 LIGAND. It is found on mature B-LYMPHOCYTES and some EPITHELIAL CELLS, lymphoid DENDRITIC CELLS. Evidence suggests that CD40-dependent activation of B-cells is important for generation of memory B-cells within the germinal centers. Mutations of the gene for CD40 antigen result in HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 3. Signaling of the receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
A membrane glycoprotein and differentiation antigen expressed on the surface of T-cells that binds to CD40 ANTIGENS on B-LYMPHOCYTES and induces their proliferation. Mutation of the gene for CD40 ligand is a cause of HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 1.
Unglycosylated phosphoproteins expressed only on B-cells. They are regulators of transmembrane Ca2+ conductance and thought to play a role in B-cell activation and proliferation.
Substances elaborated by viruses that have antigenic activity.
Costimulatory T-LYMPHOCYTE receptors that have specificity for CD80 ANTIGEN and CD86 ANTIGEN. Activation of this receptor results in increased T-cell proliferation, cytokine production and promotion of T-cell survival.
Acidic sulfated integral membrane glycoproteins expressed in several alternatively spliced and variable glycosylated forms on a wide variety of cell types including mature T-cells, B-cells, medullary thymocytes, granulocytes, macrophages, erythrocytes, and fibroblasts. CD44 antigens are the principle cell surface receptors for hyaluronate and this interaction mediates binding of lymphocytes to high endothelial venules. (From Abbas et al., Cellular and Molecular Immunology, 2d ed, p156)
Differentiation antigens expressed on pluripotential hematopoietic cells, most human thymocytes, and a major subset of peripheral blood T-lymphocytes. They have been implicated in integrin-mediated cellular adhesion and as signalling receptors on T-cells.
Glycolipid-anchored membrane glycoproteins expressed on cells of the myelomonocyte lineage including monocytes, macrophages, and some granulocytes. They function as receptors for the complex of lipopolysaccharide (LPS) and LPS-binding protein.
Glycoprotein members of the immunoglobulin superfamily which participate in T-cell adhesion and activation. They are expressed on most peripheral T-lymphocytes, natural killer cells, and thymocytes, and function as co-receptors or accessory molecules in the T-cell receptor complex.
Ratio of T-LYMPHOCYTES that express the CD4 ANTIGEN to those that express the CD8 ANTIGEN. This value is commonly assessed in the diagnosis and staging of diseases affecting the IMMUNE SYSTEM including HIV INFECTIONS.
Glycoproteins expressed on all mature T-cells, thymocytes, and a subset of mature B-cells. Antibodies specific for CD5 can enhance T-cell receptor-mediated T-cell activation. The B-cell-specific molecule CD72 is a natural ligand for CD5. (From Abbas et al., Cellular and Molecular Immunology, 2d ed, p156)
Antigens expressed primarily on the membranes of living cells during sequential stages of maturation and differentiation. As immunologic markers they have high organ and tissue specificity and are useful as probes in studies of normal cell development as well as neoplastic transformation.
A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.
Glycoproteins expressed on cortical thymocytes and on some dendritic cells and B-cells. Their structure is similar to that of MHC Class I and their function has been postulated as similar also. CD1 antigens are highly specific markers for human LANGERHANS CELLS.
Antibodies produced by a single clone of cells.
The 140 kDa isoform of NCAM (neural cell adhesion molecule) containing a transmembrane domain and short cytoplasmic tail. It is expressed by all lymphocytes mediating non-MHC restricted cytotoxicity and is present on some neural tissues and tumors.
Antigens expressed on the cell membrane of T-lymphocytes during differentiation, activation, and normal and neoplastic transformation. Their phenotypic characterization is important in differential diagnosis and studies of thymic ontogeny and T-cell function.
A membrane-bound or cytosolic enzyme that catalyzes the synthesis of CYCLIC ADP-RIBOSE (cADPR) from nicotinamide adenine dinucleotide (NAD). This enzyme generally catalyzes the hydrolysis of cADPR to ADP-RIBOSE, as well, and sometimes the synthesis of cyclic ADP-ribose 2' phosphate (2'-P-cADPR) from NADP.
Surface antigens expressed on myeloid cells of the granulocyte-monocyte-histiocyte series during differentiation. Analysis of their reactivity in normal and malignant myelomonocytic cells is useful in identifying and classifying human leukemias and lymphomas.
A costimulatory ligand expressed by ANTIGEN-PRESENTING CELLS that binds to CTLA-4 ANTIGEN with high specificity and to CD28 ANTIGEN with low specificity. The interaction of CD80 with CD28 ANTIGEN provides a costimulatory signal to T-LYMPHOCYTES, while its interaction with CTLA-4 ANTIGEN may play a role in inducing PERIPHERAL TOLERANCE.
Tetraspanin proteins found at high levels in cells of the lymphoid-myeloid lineage. CD53 antigens may be involved regulating the differentiation of T-LYMPHOCYTES and the activation of B-LYMPHOCYTES.
A cell adhesion protein that was originally identified as a heat stable antigen in mice. It is involved in METASTASIS and is highly expressed in many NEOPLASMS.
Zinc-binding metalloproteases that are members of the type II integral membrane metalloproteases. They are expressed by GRANULOCYTES; MONOCYTES; and their precursors as well as by various non-hematopoietic cells. They release an N-terminal amino acid from a peptide, amide or arylamide.
Any part or derivative of any protozoan that elicits immunity; malaria (Plasmodium) and trypanosome antigens are presently the most frequently encountered.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
A costimulatory ligand expressed by ANTIGEN-PRESENTING CELLS that binds to CD28 ANTIGEN with high specificity and to CTLA-4 ANTIGEN with low specificity. The interaction of CD86 with CD28 ANTIGEN provides a stimulatory signal to T-LYMPHOCYTES, while its interaction with CTLA-4 ANTIGEN may play a role in inducing PERIPHERAL TOLERANCE.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.
A tumor necrosis factor receptor subtype found in a variety of tissues and on activated LYMPHOCYTES. It has specificity for FAS LIGAND and plays a role in regulation of peripheral immune responses and APOPTOSIS. Multiple isoforms of the protein exist due to multiple ALTERNATIVE SPLICING. The activated receptor signals via a conserved death domain that associates with specific TNF RECEPTOR-ASSOCIATED FACTORS in the CYTOPLASM.
Antigens determined by leukocyte loci found on chromosome 6, the major histocompatibility loci in humans. They are polypeptides or glycoproteins found on most nucleated cells and platelets, determine tissue types for transplantation, and are associated with certain diseases.
Membrane antigens associated with maturation stages of B-lymphocytes, often expressed in tumors of B-cell origin.
High-molecular weight glycoproteins uniquely expressed on the surface of LEUKOCYTES and their hemopoietic progenitors. They contain a cytoplasmic protein tyrosine phosphatase activity which plays a role in intracellular signaling from the CELL SURFACE RECEPTORS. The CD45 antigens occur as multiple isoforms that result from alternative mRNA splicing and differential usage of three exons.
Process of classifying cells of the immune system based on structural and functional differences. The process is commonly used to analyze and sort T-lymphocytes into subsets based on CD antigens by the technique of flow cytometry.
Substances of fungal origin that have antigenic activity.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The major group of transplantation antigens in the mouse.
A 67-kDa sialic acid binding lectin that is specific for MYELOID CELLS and MONOCYTE-MACROPHAGE PRECURSOR CELLS. This protein is the smallest siglec subtype and contains a single immunoglobulin C2-set domain. It may play a role in intracellular signaling via its interaction with SHP-1 PROTEIN-TYROSINE PHOSPHATASE and SHP-2 PROTEIN-TYROSINE PHOSPHATASE.
Any part or derivative of a helminth that elicits an immune reaction. The most commonly seen helminth antigens are those of the schistosomes.
Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.
Cell-surface glycoprotein beta-chains that are non-covalently linked to specific alpha-chains of the CD11 family of leukocyte-adhesion molecules (RECEPTORS, LEUKOCYTE-ADHESION). A defect in the gene encoding CD18 causes LEUKOCYTE-ADHESION DEFICIENCY SYNDROME.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
A member of the tumor necrosis factor receptor superfamily that may play a role in the regulation of NF-KAPPA B and APOPTOSIS. They are found on activated T-LYMPHOCYTES; B-LYMPHOCYTES; NEUTROPHILS; EOSINOPHILS; MAST CELLS and NK CELLS. Overexpression of CD30 antigen in hematopoietic malignancies make the antigen clinically useful as a biological tumor marker. Signaling of the receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
Glycoproteins found on the membrane or surface of cells.
A critical subpopulation of regulatory T-lymphocytes involved in MHC Class I-restricted interactions. They include both cytotoxic T-lymphocytes (T-LYMPHOCYTES, CYTOTOXIC) and CD8+ suppressor T-lymphocytes.
Sites on an antigen that interact with specific antibodies.
A subtype of tetraspanin proteins that play a role in cell adhesion, cell motility, and tumor metastasis. CD9 antigens take part in the process of platelet activation and aggregation, the formation of paranodal junctions in neuronal tissue, and the fusion of sperm with egg.
A glycoprotein that is secreted into the luminal surface of the epithelia in the gastrointestinal tract. It is found in the feces and pancreaticobiliary secretions and is used to monitor the response to colon cancer treatment.
A subclass of HLA-D antigens that consist of alpha and beta chains. The inheritance of HLA-DR antigens differs from that of the HLA-DQ ANTIGENS and HLA-DP ANTIGENS.
A trisaccharide antigen expressed on glycolipids and many cell-surface glycoproteins. In the blood the antigen is found on the surface of NEUTROPHILS; EOSINOPHILS; and MONOCYTES. In addition, CD15 antigen is a stage-specific embryonic antigen.
Those proteins recognized by antibodies from serum of animals bearing tumors induced by viruses; these proteins are presumably coded for by the nucleic acids of the same viruses that caused the neoplastic transformation.
Established cell cultures that have the potential to propagate indefinitely.
A sialic acid-rich protein and an integral cell membrane mucin. It plays an important role in activation of T-LYMPHOCYTES.
Leukocyte differentiation antigens and major platelet membrane glycoproteins present on MONOCYTES; ENDOTHELIAL CELLS; PLATELETS; and mammary EPITHELIAL CELLS. They play major roles in CELL ADHESION; SIGNAL TRANSDUCTION; and regulation of angiogenesis. CD36 is a receptor for THROMBOSPONDINS and can act as a scavenger receptor that recognizes and transports oxidized LIPOPROTEINS and FATTY ACIDS.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A group of three different alpha chains (CD11a, CD11b, CD11c) that are associated with an invariant CD18 beta chain (ANTIGENS, CD18). The three resulting leukocyte-adhesion molecules (RECEPTORS, LEUKOCYTE ADHESION) are LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN-1; MACROPHAGE-1 ANTIGEN; and ANTIGEN, P150,95.
Large, transmembrane, non-covalently linked glycoproteins (alpha and beta). Both chains can be polymorphic although there is more structural variation in the beta chains. The class II antigens in humans are called HLA-D ANTIGENS and are coded by a gene on chromosome 6. In mice, two genes named IA and IE on chromosome 17 code for the H-2 antigens. The antigens are found on B-lymphocytes, macrophages, epidermal cells, and sperm and are thought to mediate the competence of and cellular cooperation in the immune response. The term IA antigens used to refer only to the proteins encoded by the IA genes in the mouse, but is now used as a generic term for any class II histocompatibility antigen.
A group of antigens that includes both the major and minor histocompatibility antigens. The former are genetically determined by the major histocompatibility complex. They determine tissue type for transplantation and cause allograft rejections. The latter are systems of allelic alloantigens that can cause weak transplant rejection.
Small glycoproteins found on both hematopoietic and non-hematopoietic cells. CD59 restricts the cytolytic activity of homologous complement by binding to C8 and C9 and blocking the assembly of the membrane attack complex. (From Barclay et al., The Leukocyte Antigen FactsBook, 1993, p234)
IMMUNOGLOBULINS on the surface of B-LYMPHOCYTES. Their MESSENGER RNA contains an EXON with a membrane spanning sequence, producing immunoglobulins in the form of type I transmembrane proteins as opposed to secreted immunoglobulins (ANTIBODIES) which do not contain the membrane spanning segment.
Nuclear antigen with a role in DNA synthesis, DNA repair, and cell cycle progression. PCNA is required for the coordinated synthesis of both leading and lagging strands at the replication fork during DNA replication. PCNA expression correlates with the proliferation activity of several malignant and non-malignant cell types.
Oligosaccharide antigenic determinants found principally on NK cells and T-cells. Their role in the immune response is poorly understood.
A transmembrane protein belonging to the tumor necrosis factor superfamily that specifically binds to CD27 ANTIGEN. It is found on activated T-LYMPHOCYTES; B-LYMPHOCYTES; and DENDRITIC CELLS where it plays a role in stimulating the proliferation of CD4-POSITIVE T-LYMPHOCYTES and CD8-POSITIVE T-LYMPHOCYTES.
A ubiquitously expressed complement receptor that binds COMPLEMENT C3B and COMPLEMENT C4B and serves as a cofactor for their inactivation. CD46 also interacts with a wide variety of pathogens and mediates immune response.
A class of animal lectins that bind to carbohydrate in a calcium-dependent manner. They share a common carbohydrate-binding domain that is structurally distinct from other classes of lectins.
Glycoproteins with a wide distribution on hematopoietic and non-hematopoietic cells and strongly expressed on macrophages. CD58 mediates cell adhesion by binding to CD2; (ANTIGENS, CD2); and this enhances antigen-specific T-cell activation.
55-kDa antigens found on HELPER-INDUCER T-LYMPHOCYTES and on a variety of other immune cell types. CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in MAJOR HISTOCOMPATIBILITY COMPLEX class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as INTERLEUKIN-15 receptors and bind to the HIV receptors, binding directly to the HIV ENVELOPE PROTEIN GP120.
A ubiquitously expressed membrane glycoprotein. It interacts with a variety of INTEGRINS and mediates responses to EXTRACELLULAR MATRIX PROTEINS.
A CD antigen that contains a conserved I domain which is involved in ligand binding. When combined with CD18 the two subunits form MACROPHAGE-1 ANTIGEN.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A glycoprotein that is a kallikrein-like serine proteinase and an esterase, produced by epithelial cells of both normal and malignant prostate tissue. It is an important marker for the diagnosis of prostate cancer.
An integrin alpha subunit of approximately 150-kDa molecular weight. It is expressed at high levels on monocytes and combines with CD18 ANTIGEN to form the cell surface receptor INTEGRIN ALPHAXBETA2. The subunit contains a conserved I-domain which is characteristic of several of alpha integrins.
The lipopolysaccharide-protein somatic antigens, usually from gram-negative bacteria, important in the serological classification of enteric bacilli. The O-specific chains determine the specificity of the O antigens of a given serotype. O antigens are the immunodominant part of the lipopolysaccharide molecule in the intact bacterial cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
A specific HLA-A surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-A*02 allele family.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Progenitor cells from which all blood cells derive.
The number of CD4-POSITIVE T-LYMPHOCYTES per unit volume of BLOOD. Determination requires the use of a fluorescence-activated flow cytometer.
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
Carbohydrate antigens expressed by malignant tissue. They are useful as tumor markers and are measured in the serum by means of a radioimmunoassay employing monoclonal antibodies.
GPI-linked membrane proteins broadly distributed among hematopoietic and non-hematopoietic cells. CD55 prevents the assembly of C3 CONVERTASE or accelerates the disassembly of preformed convertase, thus blocking the formation of the membrane attack complex.
Cell adhesion molecules present on virtually all monocytes, platelets, and granulocytes. CD31 is highly expressed on endothelial cells and concentrated at the junctions between them.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Membrane glycoproteins consisting of an alpha subunit and a BETA 2-MICROGLOBULIN beta subunit. In humans, highly polymorphic genes on CHROMOSOME 6 encode the alpha subunits of class I antigens and play an important role in determining the serological specificity of the surface antigen. Class I antigens are found on most nucleated cells and are generally detected by their reactivity with alloantisera. These antigens are recognized during GRAFT REJECTION and restrict cell-mediated lysis of virus-infected cells.
Tetraspanin proteins that are involved in a variety of cellular functions including BASEMENT MEMBRANE assembly, and in the formation of a molecular complexes on the surface of LYMPHOCYTES.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
A member of the tumor necrosis factor receptor superfamily that is specific for 4-1BB LIGAND. It is found in a variety of immune cell types including activated T-LYMPHOCYTES; NATURAL KILLER CELLS; and DENDRITIC CELLS. Activation of the receptor on T-LYMPHOCYTES plays a role in their expansion, production of cytokines and survival. Signaling by the activated receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Proteins prepared by recombinant DNA technology.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.
Polymorphic class I human histocompatibility (HLA) surface antigens present on almost all nucleated cells. At least 20 antigens have been identified which are encoded by the A locus of multiple alleles on chromosome 6. They serve as targets for T-cell cytolytic responses and are involved with acceptance or rejection of tissue/organ grafts.
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).
Receptors present on activated T-LYMPHOCYTES and B-LYMPHOCYTES that are specific for INTERLEUKIN-2 and play an important role in LYMPHOCYTE ACTIVATION. They are heterotrimeric proteins consisting of the INTERLEUKIN-2 RECEPTOR ALPHA SUBUNIT, the INTERLEUKIN-2 RECEPTOR BETA SUBUNIT, and the INTERLEUKIN RECEPTOR COMMON GAMMA-CHAIN.
Sets of cell surface antigens located on BLOOD CELLS. They are usually membrane GLYCOPROTEINS or GLYCOLIPIDS that are antigenically distinguished by their carbohydrate moieties.
Those hepatitis B antigens found on the surface of the Dane particle and on the 20 nm spherical and tubular particles. Several subspecificities of the surface antigen are known. These were formerly called the Australia antigen.
Ubiquitously-expressed tetraspanin proteins that are found in late ENDOSOMES and LYSOSOMES and have been implicated in intracellular transport of proteins.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Tetraspanin proteins found associated with LAMININ-binding INTEGRINS. The CD151 antigens may play a role in the regulation of CELL MOTILITY.
A component of the B-cell antigen receptor that is involved in B-cell antigen receptor heavy chain transport to the PLASMA MEMBRANE. It is expressed almost exclusively in B-LYMPHOCYTES and serves as a useful marker for B-cell NEOPLASMS.
An encapsulated lymphatic organ through which venous blood filters.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Human immune-response or Class II antigens found mainly, but not exclusively, on B-lymphocytes and produced from genes of the HLA-D locus. They are extremely polymorphic families of glycopeptides, each consisting of two chains, alpha and beta. This group of antigens includes the -DR, -DQ and -DP designations, of which HLA-DR is most studied; some of these glycoproteins are associated with certain diseases, possibly of immune etiology.
A membrane-bound tumor necrosis family member found primarily on activated T-LYMPHOCYTES that binds specifically to CD30 ANTIGEN. It may play a role in INFLAMMATION and immune regulation.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
A class of enzymes involved in the hydrolysis of the N-glycosidic bond of nitrogen-linked sugars.
A form of undifferentiated malignant LYMPHOMA usually found in central Africa, but also reported in other parts of the world. It is commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. B-cell antigens are expressed on the immature cells that make up the tumor in virtually all cases of Burkitt lymphoma. The Epstein-Barr virus (HERPESVIRUS 4, HUMAN) has been isolated from Burkitt lymphoma cases in Africa and it is implicated as the causative agent in these cases; however, most non-African cases are EBV-negative.
Molecules on the surface of B- and T-lymphocytes that recognize and combine with specific antigens.
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.
An alpha-integrin subunit found on lymphocytes, granulocytes, macrophages and monocytes. It combines with the integrin beta2 subunit (CD18 ANTIGEN) to form LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN-1.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Antigens of the virion of the HEPATITIS B VIRUS or the Dane particle, its surface (HEPATITIS B SURFACE ANTIGENS), core (HEPATITIS B CORE ANTIGENS), and other associated antigens, including the HEPATITIS B E ANTIGENS.
The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.
The processes triggered by interactions of ANTIBODIES with their ANTIGENS.
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
Mice homozygous for the mutant autosomal recessive gene "scid" which is located on the centromeric end of chromosome 16. These mice lack mature, functional lymphocytes and are thus highly susceptible to lethal opportunistic infections if not chronically treated with antibiotics. The lack of B- and T-cell immunity resembles severe combined immunodeficiency (SCID) syndrome in human infants. SCID mice are useful as animal models since they are receptive to implantation of a human immune system producing SCID-human (SCID-hu) hematochimeric mice.
Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
A heterogeneous group of immunocompetent cells that mediate the cellular immune response by processing and presenting antigens to the T-cells. Traditional antigen-presenting cells include MACROPHAGES; DENDRITIC CELLS; LANGERHANS CELLS; and B-LYMPHOCYTES. FOLLICULAR DENDRITIC CELLS are not traditional antigen-presenting cells, but because they hold antigen on their cell surface in the form of IMMUNE COMPLEXES for B-cell recognition they are considered so by some authors.
The type species of LYMPHOCRYPTOVIRUS, subfamily GAMMAHERPESVIRINAE, infecting B-cells in humans. It is thought to be the causative agent of INFECTIOUS MONONUCLEOSIS and is strongly associated with oral hairy leukoplakia (LEUKOPLAKIA, HAIRY;), BURKITT LYMPHOMA; and other malignancies.
T-cell receptors composed of CD3-associated alpha and beta polypeptide chains and expressed primarily in CD4+ or CD8+ T-cells. Unlike immunoglobulins, the alpha-beta T-cell receptors recognize antigens only when presented in association with major histocompatibility (MHC) molecules.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Class I human histocompatibility (HLA) surface antigens encoded by more than 30 detectable alleles on locus B of the HLA complex, the most polymorphic of all the HLA specificities. Several of these antigens (e.g., HLA-B27, -B7, -B8) are strongly associated with predisposition to rheumatoid and other autoimmune disorders. Like other class I HLA determinants, they are involved in the cellular immune reactivity of cytolytic T lymphocytes.
The altered state of immunologic responsiveness resulting from initial contact with antigen, which enables the individual to produce antibodies more rapidly and in greater quantity in response to secondary antigenic stimulus.
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
The phenomenon of target cell destruction by immunologically active effector cells. It may be brought about directly by sensitized T-lymphocytes or by lymphoid or myeloid "killer" cells, or it may be mediated by cytotoxic antibody, cytotoxic factor released by lymphoid cells, or complement.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
A melanosome-specific protein that plays a role in the expression, stability, trafficking, and processing of GP100 MELANOMA ANTIGEN, which is critical to the formation of Stage II MELANOSOMES. The protein is used as an antigen marker for MELANOMA cells.
A widely distributed cell surface transmembrane glycoprotein that stimulates the synthesis of MATRIX METALLOPROTEINASES. It is found at high levels on the surface of malignant NEOPLASMS and may play a role as a mediator of malignant cell behavior.
A general term for various neoplastic diseases of the lymphoid tissue.
An albumin obtained from the white of eggs. It is a member of the serpin superfamily.
Antigens associated with specific proteins of the human adult T-cell immunodeficiency virus (HIV); also called HTLV-III-associated and lymphadenopathy-associated virus (LAV) antigens.
An inhibitory T CELL receptor that is closely related to CD28 ANTIGEN. It has specificity for CD80 ANTIGEN and CD86 ANTIGEN and acts as a negative regulator of peripheral T cell function. CTLA-4 antigen is believed to play role in inducing PERIPHERAL TOLERANCE.
A promyelocytic cell line derived from a patient with ACUTE PROMYELOCYTIC LEUKEMIA. HL-60 cells lack specific markers for LYMPHOID CELLS but express surface receptors for FC FRAGMENTS and COMPLEMENT SYSTEM PROTEINS. They also exhibit phagocytic activity and responsiveness to chemotactic stimuli. (From Hay et al., American Type Culture Collection, 7th ed, pp127-8)
A widely expressed transmembrane glycoprotein that functions as a METASTASIS suppressor protein. It is underexpressed in a variety of human NEOPLASMS.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
A group of differentiation surface antigens, among the first to be discovered on thymocytes and T-lymphocytes. Originally identified in the mouse, they are also found in other species including humans, and are expressed on brain neurons and other cells.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
The specific failure of a normally responsive individual to make an immune response to a known antigen. It results from previous contact with the antigen by an immunologically immature individual (fetus or neonate) or by an adult exposed to extreme high-dose or low-dose antigen, or by exposure to radiation, antimetabolites, antilymphocytic serum, etc.
Manifestations of the immune response which are mediated by antigen-sensitized T-lymphocytes via lymphokines or direct cytotoxicity. This takes place in the absence of circulating antibody or where antibody plays a subordinate role.
A single, unpaired primary lymphoid organ situated in the MEDIASTINUM, extending superiorly into the neck to the lower edge of the THYROID GLAND and inferiorly to the fourth costal cartilage. It is necessary for normal development of immunologic function early in life. By puberty, it begins to involute and much of the tissue is replaced by fat.
Endogenous tissue constituents that have the ability to interact with AUTOANTIBODIES and cause an immune response.
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
Nuclear antigens encoded by VIRAL GENES found in HUMAN HERPESVIRUS 4. At least six nuclear antigens have been identified.
A soluble substance elaborated by antigen- or mitogen-stimulated T-LYMPHOCYTES which induces DNA synthesis in naive lymphocytes.
A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.
A cell line derived from cultured tumor cells.
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
A sex-specific cell surface antigen produced by the sex-determining gene of the Y chromosome in mammals. It causes syngeneic grafts from males to females to be rejected and interacts with somatic elements of the embryologic undifferentiated gonad to produce testicular organogenesis.
A cell adhesion molecule of the immunoglobulin superfamily that is expressed in ENDOTHELIAL CELLS and is involved in INTERCELLULAR JUNCTIONS.
Antigens stimulating the formation of, or combining with heterophile antibodies. They are cross-reacting antigens found in phylogenetically unrelated species.
CD4-positive T cells that inhibit immunopathology or autoimmune disease in vivo. They inhibit the immune response by influencing the activity of other cell types. Regulatory T-cells include naturally occurring CD4+CD25+ cells, IL-10 secreting Tr1 cells, and Th3 cells.
Antibodies obtained from a single clone of cells grown in mice or rats.
Antigenic determinants recognized and bound by the T-cell receptor. Epitopes recognized by the T-cell receptor are often located in the inner, unexposed side of the antigen, and become accessible to the T-cell receptors after proteolytic processing of the antigen.
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
A heterodimeric protein that is a cell surface antigen associated with lymphocyte activation. The initial characterization of this protein revealed one identifiable heavy chain (ANTIGENS, CD98 HEAVY CHAIN) and an indeterminate smaller light chain. It is now known that a variety of light chain subunits (ANTIGENS, CD98 LIGHT CHAINS) can dimerize with the heavy chain. Depending upon its light chain composition a diverse array of functions can be found for this protein. Functions include: type L amino acid transport, type y+L amino acid transport and regulation of cellular fusion.
The hepatitis B antigen within the core of the Dane particle, the infectious hepatitis virion.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
The complex formed by the binding of antigen and antibody molecules. The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES.
They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system.
The sum of the weight of all the atoms in a molecule.
Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.
A group of the D-related HLA antigens found to differ from the DR antigens in genetic locus and therefore inheritance. These antigens are polymorphic glycoproteins comprising alpha and beta chains and are found on lymphoid and other cells, often associated with certain diseases.
Immunoglobulins produced in response to VIRAL ANTIGENS.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
A glycolipid, cross-species antigen that induces production of antisheep hemolysin. It is present on the tissue cells of many species but absent in humans. It is found in many infectious agents.
Elements of limited time intervals, contributing to particular results or situations.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
An inhibitory B7 antigen that has specificity for the T-CELL receptor PROGRAMMED CELL DEATH 1 PROTEIN. CD274 antigen provides negative signals that control and inhibit T-cell responses and is found at higher than normal levels on tumor cells, suggesting its potential role in TUMOR IMMUNE EVASION.
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
A species of POLYOMAVIRUS originally isolated from Rhesus monkey kidney tissue. It produces malignancy in human and newborn hamster kidney cell cultures.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
Substances that augment, stimulate, activate, potentiate, or modulate the immune response at either the cellular or humoral level. The classical agents (Freund's adjuvant, BCG, Corynebacterium parvum, et al.) contain bacterial antigens. Some are endogenous (e.g., histamine, interferon, transfer factor, tuftsin, interleukin-1). Their mode of action is either non-specific, resulting in increased immune responsiveness to a wide variety of antigens, or antigen-specific, i.e., affecting a restricted type of immune response to a narrow group of antigens. The therapeutic efficacy of many biological response modifiers is related to their antigen-specific immunoadjuvanticity.
Antigens that exist in alternative (allelic) forms in a single species. When an isoantigen is encountered by species members who lack it, an immune response is induced. Typical isoantigens are the BLOOD GROUP ANTIGENS.
Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell.
A melanosome-associated protein that plays a role in the maturation of the MELANOSOME.
The genetic region which contains the loci of genes which determine the structure of the serologically defined (SD) and lymphocyte-defined (LD) TRANSPLANTATION ANTIGENS, genes which control the structure of the IMMUNE RESPONSE-ASSOCIATED ANTIGENS, HUMAN; the IMMUNE RESPONSE GENES which control the ability of an animal to respond immunologically to antigenic stimuli, and genes which determine the structure and/or level of the first four components of complement.
Bone marrow-derived lymphocytes that possess cytotoxic properties, classically directed against transformed and virus-infected cells. Unlike T CELLS; and B CELLS; NK CELLS are not antigen specific. The cytotoxicity of natural killer cells is determined by the collective signaling of an array of inhibitory and stimulatory CELL SURFACE RECEPTORS. A subset of T-LYMPHOCYTES referred to as NATURAL KILLER T CELLS shares some of the properties of this cell type.
A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
Form of passive immunization where previously sensitized immunologic agents (cells or serum) are transferred to non-immune recipients. When transfer of cells is used as a therapy for the treatment of neoplasms, it is called adoptive immunotherapy (IMMUNOTHERAPY, ADOPTIVE).

RhoA activity is required for fibronectin assembly and counteracts beta1B integrin inhibitory effect in FRT epithelial cells. (1/1924)

FRT thyroid epithelial cells synthesize fibronectin and organize a network of fibronectin fibrils at the basal surface of the cells. Fibronectin fibril formation is enhanced by the overexpression of the ubiquitous beta1A integrin and is inhibited by the expression of the dominant-negative beta1B subunit. We tested the hypotheses that RhoA activity might mediate the integrin-dependent fibronectin fibrillogenesis and might counteract beta1B integrin inhibitory effect. FRT-beta1A cells were transfected with a vector carrying a dominant negative form of RhoA (RhoAN19) or treated with the C3 transferase exoenzyme. Both treatments inhibited fibronectin assembly and caused loss of actin microfilaments and adhesion plaques. On the other hand, FRT-beta1B cells were transfected with the constitutively activated form of RhoA (RhoAV14) or treated with the E. coli cytotoxic necrotizing factor 1, which directly activates RhoA. Either treatment restored microfilament and adhesion plaque assembly and promoted fibronectin fibril organization. A great increase in fibronectin fibril assembly was also obtained by treatment of FRT-beta1B cells with TGF-beta. Our data indicate that RhoA is required to promote fibronectin matrix assembly in FRT cells and that the activation of the signal transduction pathway downstream of RhoA can overcome the inhibitory effect of beta1B integrin.  (+info)

A region of the Yersinia pseudotuberculosis invasin protein enhances integrin-mediated uptake into mammalian cells and promotes self-association. (2/1924)

Invasin allows efficient entry into mammalian cells by Yersinia pseudotuberculosis. It has been shown that the C-terminal 192 amino acids of invasin are essential for binding of beta1 integrin receptors and subsequent uptake. By analyzing the internalization of latex beads coated with invasin derivatives, an additional domain of invasin was shown to be required for efficient bacterial internalization. A monomeric derivative encompassing the C-terminal 197 amino acids was inefficient at promoting entry of latex beads, whereas dimerization of this derivative by antibody significantly increased uptake. By using the DNA-binding domain of lambda repressor as a reporter for invasin self-interaction, we have demonstrated that a region of the invasin protein located N-terminal to the cell adhesion domain of invasin is able to self-associate. Chemical cross-linking studies of purified and surface-exposed invasin proteins, and the dominant-interfering effect of a non-functional invasin derivative are consistent with the presence of a self-association domain that is located within the region of invasin that enhances bacterial uptake. We conclude that interaction of homomultimeric invasin with multiple integrins establishes tight adherence and receptor clustering, thus providing a signal for internalization.  (+info)

CD9 is involved in invasion of human trophoblast-like choriocarcinoma cell line, BeWo cells. (3/1924)

The CD9 molecule is expressed on human extravillous trophoblasts, which invade the endometrium during implantation and placentation. To elucidate the role of CD9 in trophoblastic function, we investigated the expression of CD9 protein and mRNA in BeWo cells, a human trophoblast-like choriocarcinoma cell line, using immunohistochemistry, Western blotting and reverse transcription-polymerase chain reaction (RT-PCR). When BeWo cells were cultured with anti-CD9 monoclonal antibodies (mAb), their invasion through the extracellular matrices was significantly enhanced in a dose-dependent manner. Cell proliferation and human chorionic gonadotrophin production were unaffected. On the other hand, culture in the presence of mAb against integrins alpha3, alpha5 and beta1, which partially block the interaction with the extracellular matrices, inhibited BeWo cell invasion. Anti-CD9 monoclonal antibody had a stimulatory effect on BeWo cell invasion in the presence of anti-integrin alpha3 antibody. In contrast, it had no effect in the presence of mAb against integrins alpha5 and beta1, which were also highly expressed on BeWo cells. These findings suggest that CD9 has a function connected with the invasive properties of BeWo cells, which is partially mediated by integrin alpha5beta1. This may relate to the involvement of CD9 in trophoblastic invasion.  (+info)

Minimally modified low-density lipoprotein induces monocyte adhesion to endothelial connecting segment-1 by activating beta1 integrin. (4/1924)

We have shown previously that treatment of human aortic endothelial cells (HAECs) with minimally modified low-density lipoprotein (MM-LDL) induces monocyte but not neutrophil binding. This monocyte binding was not mediated by endothelial E-selectin, P-selectin, vascular cell adhesion molecule-I, or intercellular adhesion molecule-I, suggesting an alternative monocyte-specific adhesion molecule. We now show that moncytic alpha4beta1 integrins mediate binding to MM-LDL-treated endothelial cells. We present data suggesting that the expression of the connecting segment-1 (CS-1) domain of fibronectin (FN) is induced on the apical surface of HAEC by MM-LDL and is the endothelial alpha4beta1 ligand in MM-LDL-treated cells. Although the levels of CS-1 mRNA and protein were not increased, we show that MM-LDL treatment causes deposition of FN on the apical surface by activation of beta1integrins, particularly those associated with alpha5 integrins. Activation of beta1 by antibody 8A2 also induced CS-1-mediated monocyte binding. Confocal microscopy demonstrated the activated beta1 and CS-1colocalize in concentrated filamentous patches on the apical surface of HAEC. Both anti-CS-1 and an antibody to activated beta1 showed increased staining on the luminal endothelium of human coronary lesions with active monocyte entry. These results suggest the importance of these integrin ligand interactions in human atherosclerosis.  (+info)

Mutational analysis of cell cycle inhibition by integrin beta1C. (5/1924)

Integrin beta1C is an alternatively spliced cytoplasmic variant of the beta1 subunit that potently inhibits cell cycle progression. In this study, we analyzed the requirements for growth suppression by beta1C. A chimera containing the extracellular/transmembrane domain of the Tac subunit of the human interleukin 2 receptor (gp55) fused to the cytoplasmic domain of beta1C (residues 732-805) strongly inhibited growth in mouse 10T1/2 cells even at low expression levels, whereas chimeras containing the beta1A, beta1B, beta1D, beta3, and beta5 cytoplasmic domains had weak and variable effects. The beta1C cytoplasmic domain is composed of a membrane proximal region (732-757) common to all beta1 variants and a COOH-terminal 48-amino acid domain (758-805) unique to beta1C. The beta1C-specific domain (758-805) was sufficient to block cell growth even when expressed as a soluble cytoplasmic green fluorescent protein fusion protein. These results indicate that growth inhibition by beta1C does not require the intact receptor and can function in the absence of membrane targeting. Analysis of deletions within the beta1C-specific domain showed that the 18-amino acid sequence 775-792 is both necessary and sufficient for maximal growth inhibition, although the 13 COOH-terminal residues (793-805) also had weak activity. Finally, beta1C is known to be induced in endothelial cells in response to tumor necrosis factor and is down-regulated in prostate epithelial cells after transformation. The green fluorescent protein/beta1C (758-805) chimera blocked growth in the human endothelial cell line EV304 and in the transformed prostate epithelial cell line DU145, consistent with a role for beta1C as a growth inhibitor in vivo.  (+info)

Mouse primordial germ cells lacking beta1 integrins enter the germline but fail to migrate normally to the gonads. (6/1924)

Primordial germ cells are the founder cells of the gametes. They are set aside at the initial stages of gastrulation in mammals, become embedded in the hind-gut endoderm, then actively migrate to the sites of gonad formation. The molecular basis of this migration is poorly understood. Here we sought to determine if members of the integrin family of cell surface receptors are required for primordial germ cell migration, as integrins have been implicated in the migration of several other motile cell types. We have established a line of mice which express green fluorescent protein in germline cells that has enabled us to efficiently purify primordial germ cells at different stages by flow cytometry. We have catalogued the spectrum of integrin subunit expression by primordial germ cells during and after migration, using flow cytometry, immunocytochemistry and RT-PCR. Through analysis of integrin beta1(-/-)-->wild-type chimeras, we show that embryonic cells lacking beta1 integrins can enter the germline. However, integrin beta1(-/-) primordial germ cells do not colonize the gonad efficiently. Embryos with targeted deletion of integrin subunit alpha3, alpha6, or alphaV show no major defects in primordial germ cell migration. These results demonstrate a role for beta1-containing integrins in the development of the germline, although an equivalent role for * integrin subunit(s) has yet to be established.  (+info)

Maturation of the myogenic program is induced by postmitotic expression of insulin-like growth factor I. (7/1924)

The molecular mechanisms underlying myogenic induction by insulin-like growth factor I (IGF-I) are distinct from its proliferative effects on myoblasts. To determine the postmitotic role of IGF-I on muscle cell differentiation, we derived L6E9 muscle cell lines carrying a stably transfected rat IGF-I gene under the control of a myosin light chain (MLC) promoter-enhancer cassette. Expression of MLC-IGF-I exclusively in differentiated L6E9 myotubes, which express the embryonic form of myosin heavy chain (MyHC) and no endogenous IGF-I, resulted in pronounced myotube hypertrophy, accompanied by activation of the neonatal MyHC isoform. The hypertrophic myotubes dramatically increased expression of myogenin, muscle creatine kinase, beta-enolase, and IGF binding protein 5 and activated the myocyte enhancer factor 2C gene which is normally silent in this cell line. MLC-IGF-I induction in differentiated L6E9 cells also increased the expression of a transiently transfected LacZ reporter driven by the myogenin promoter, demonstrating activation of the differentiation program at the transcriptional level. Nuclear reorganization, accumulation of skeletal actin protein, and an increased expression of beta1D integrin were also observed. Inhibition of the phosphatidyl inositol (PI) 3-kinase intermediate in IGF-I-mediated signal transduction confirmed that the PI 3-kinase pathway is required only at early stages for IGF-I-mediated hypertrophy and neonatal MyHC induction in these cells. Expression of IGF-I in postmitotic muscle may therefore play an important role in the maturation of the myogenic program.  (+info)

Targeted disruption of fibronectin-integrin interactions in human gingival fibroblasts by the RI protease of Porphyromonas gingivalis W50. (8/1924)

Cell surface integrins mediate interactions between cells and their extracellular matrix and are frequently exploited by a range of bacterial pathogens to facilitate adherence and/or invasion. In this study we examined the effects of Porphyromonas gingivalis proteases on human gingival fibroblast (HGF) integrins and their fibronectin matrix. Culture supernatant from the virulent strain W50 caused considerably greater loss of the beta1 integrin subunit from HGF in vitro than did that of the beige-pigmented strain W50/BE1. Prior treatment of the W50 culture supernatant with the protease inhibitor Nalpha-p-tosyl-L-lysine chloromethyl ketone (TLCK) blocked its effects on cultured cells, indicating that this process is proteolytically mediated. Purified arginine-specific proteases from P. gingivalis W50 were able to mimic the effects of the whole-culture supernatant on loss of beta1 integrin expression. However purified RI, an alpha/beta heterodimer in which the catalytic chain is associated with an adhesin chain, was 12 times more active than RIA, the catalytic monomer, in causing loss of the alpha5beta1 integrin (fibronectin receptor) from HGF. No effect was observed on the alphaVbeta3 integrin (vitronectin receptor). The sites of action of RI and RIA were investigated in cells exposed to proteases pretreated with TLCK to inactivate the catalytic component. Use of both monoclonal antibody 1A1, which recognizes only the adhesin chain of RI, and a rabbit antibody against P. gingivalis whole cells indicated localization of RI on the fibroblasts in a clear, linear pattern typical of that seen with fibronectin and alpha5beta1 integrin. Exact colocalization of RI with fibronectin and its alpha5beta1 receptor was confirmed by double labeling and multiple-exposure photomicroscopy. In contrast, RIA bound to fibroblasts in a weak, patchy manner, showing only fine linear or granular staining. It is concluded that the adhesin component of RI targets the P. gingivalis arginine-protease to sites of fibronectin deposition on HGF, contributing to the rapid loss of both fibronectin and its main alpha5beta1 integrin receptor. Given the importance of integrin-ligand interactions in fibroblast function, their targeted disruption by RI may represent a novel mechanism of damage in periodontal disease.  (+info)

CD49d / integrin alpha 4, unlike other alpha integrins, neither contains an I-domain, nor undergoes disulfide-linked cleavage. It associates with beta 7 chain to form alpha 4 / beta 7 integrin, and with beta 1 chain (CD29) to form VLA-4 integrin. These complexes are important for lymphocyte migration from circulation into tissue (binding VCAM-1) and homing of T cell subsets to Peyer s patches (binding MadCAM-1), but VLA-4 is also target for invasive bacteria which contain invasin. CD49d is essential for differentiation and migration of hematopoietic stem cells by their adhesion to bone marrow stromal cells, and provides a costimulatory signal to TCR-CD3 complex by inducing phosphorylation of some focal adhesion proteins ...
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Integrins beta 5, beta 3 and alpha v are apically distributed in endometrial epithelium is an eagle-i resource of type Journal article at eagle-i Network Shared Resource Repository.
Biophysical Stimulation Induces Schwann Cell Proliferation and Demyelination via an Integrin-Dependent Mechanism in an In-Vitro Model of Chronic Nerve Compression ...
TY - JOUR. T1 - Subclassification, molecular structure, function and ligand in integrin superfamily. AU - Matsuura, N.. AU - Takada, Y.. PY - 1995/7. Y1 - 1995/7. N2 - Integrins are the major family of cell surface receptors that mediate adhesion to the extracellular matrix and sometimes cell-cell adhesive interactions. These integrin-mediated adhesive interactions are involved in the regulation of many cellular functions, including embryonic development, tumor cell growth and metastasis, programmed cell death, hemostasis, inflammation, immune reaction, bone reabsorption, etc. Integrins are composed of alpha and beta transmembrane subunits selected from among 16 alpha and 8 beta subunits that heterodimerize to produce more than 20 different receptors which bind specific ligands. Ligand binding sites have been clarified by chimera integrin protein in some integrins. Integrins link to intracellular cytoskeletal complexes and bundles of actin filaments. There have been many reports about ...
Effect of blocking αvβ3 integrins on tenascin- C-dependent smooth muscle cell morphology, attachment efficiency, and survival. (A) Representative phase c
TY - JOUR. T1 - A novel activating anti-β1 integrin monoclonal antibody binds to the cysteine-rich repeats in the β1 chain. AU - Faull, Randall J.. AU - Wang, Jian. AU - Leavesley, David I.. AU - Puzon, Wilma. AU - Russ, Graeme R.. AU - Vestweber, Dietmar. AU - Takada, Yoshikazu. PY - 1996. Y1 - 1996. N2 - The functional status of an integrin depends on the conformation of its extracellular domain, which is controlled by the cell expressing that receptor. The transmission of regulatory signals from within the cell is considered to be via propagated conformational changes from the receptors cytoplasmic tails to the extracellular ligand binding pocket. The end result is increased accessibility of the ligand binding pocket in the high affinity (active) form of integrins. We report a novel monoclonal antibody (QE.2E5) that binds within the cysteine-rich repeats in the integrin β1 chain and induces high affinity binding of fibronectin to the integrin α5β1. The QE.2E5 epitope is located ...
Plasmid mEmerald-Alpha-5-Integrin-12 from Dr. Michael Davidsons lab contains the insert Alpha-5 Integrin. This plasmid is available through Addgene.
In the normal kidney, the α8 integrin chain is expressed only on mesangial cells and vascular smooth muscle cells. α8 integrin ligates several matrix molecules including fibronectin, osteopontin and fibrillin-1. Recently, we detected de novo expression of α8 integrin on epithelial cells in renal cysts. We hypothesized that the α8 integrin chain is induced in tubular epithelia undergoing dedifferentiation and contributes to the fibrotic response in the tubulointerstitium (TI) after unilateral ureteral obstruction (UUO). After induction of UUO in rats by ligation of the right ureter, increased expression of the α8 integrin chain and its ligands was observed. In the TI, α8 integrin was localized to cytokeratin-positive epithelial cells and to interstitial fibroblasts; and colocalized with its ligands. In mice underexpressing α8 integrin UUO led to collagen deposition and fibroblast activation comparable to wild types. Mice lacking α8 integrin showed even more TI damage, fibroblast activation and
Several pieces of evidence presented here document that β1Δ/Δ or Dko mice have an uncompensated anemia at homeostasis with signs of ineffective erythropoiesis and shortened RBC survival likely because of their inability to counteract chronic ROS accumulation. As a result, membrane changes through protein oxidation and lipid peroxidation would affect membrane fluidity and stability,3,4 leading to hemolysis. Since a similar picture is not seen in the absence of only α4-integrins ([α4β1;α4β7]−/−) the data would suggest that the absence of other integrin heterodimers in β1Δ/Δ or Dkos alone or in combination are responsible for this phenotype.. Integrins expressed in differentiated erythroid cells (mainly α4β1 and α5β1) and their interactions with fibronectin (Fn) in their ME have been previously emphasized as critical for completing terminal maturation steps.30,40,41 Specifically, on the basis of in vitro studies using fetal liver cells, it was concluded that Epo and Fn regulate ...
The M290 monoclonal antibody reacts with mouse CD103 also known as integrin αE (ITGAE). CD103 is an integrin protein that binds integrin beta 7 to form the complete heterodimeric integrin molecule αEβ7. CD103 is expressed widely on intraepithelial lymphocyte (IEL) T cells (both αβ T cells and γδ T cells) and on some peripheral regulatory T cells. It has also been reported on lamina propria T cells. A subset of dendritic cells in the gut mucosa and in mesenteric lymph nodes also expresses CD103. The main ligand for CD103 is E-cadherin, an adhesion molecule expressed by epithelial cells. CD103 is thought to facilitate the interactions of T cells with epithelial cells during T cell maturation and effector functions. The M290 antibody is reported to neutralize CD103 |em|in vivo|/em|.
This gene encodes a beta subunit of integrin, which can combine with different alpha chains to form a variety of integrin heterodimers. Integrins are integral cell-surface receptors that participate in cell adhesion as well as cell-surface mediated signaling. The alphav beta5 integrin is involved in adhesion to vitronectin. [provided by RefSeq, Aug 2017] ...
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1. Lu X, Lu D, Scully M, Kakkar V. The role of integrins in cancer and the development of anti-integrin therapeutic agents for cancer therapy. Perspect Medicin Chem. 2008;2:57-73 2. Hynes RO. Integrins: a family of cell surface receptors. Cell. 1987;48:549-54 3. Hynes RO. Integrins: versatility, modulation, and signaling in cell adhesion. Cell. 1992;69:11-25 4. Serini G, Valdembri D, Bussolino F. Integrins and angiogenesis: a sticky business. Exp Cell Res. 2006;312:651-8 5. Brakebusch C, Bouvard D, Stanchi F, Sakai T, Fassler R. Integrins in invasive growth. J Clin Invest. 2002;109:999-1006 6. Hynes RO. Integrins: bidirectional, allosteric signaling machines. Cell. 2002;110:673-87 7. Vogel V, Sheetz M. Local force and geometry sensing regulate cell functions. Nat Rev Mol Cell Biol. 2006;7:265-75 8. Ginsberg MH, Partridge A, Shattil SJ. Integrin regulation. Curr Opin Cell Biol. 2005;17:509-16 9. Springer TA. Complement and the multifaceted functions of VWA and integrin I domains. Structure. ...
Shop a large selection of products and learn more about Integrin beta 5 Mouse anti-Hamster, Human, Mouse, FITC, Clone: KN52, eBioscience 25 Tests; FITC.
Integrin beta 3 antibody [JM2E5] (FITC) (integrin subunit beta 3) for FACS. Anti-Integrin beta 3 mAb (GTX43357) is tested in Human, Dog, Pig, Horse, Bovine samples. 100% Ab-Assurance.
VEGFRs in PI 3-kinase and integrin activation. (A) VEGFR knockdown. HUVECs transfected with the indicated siRNAs for 72 h were analyzed for VEGFR expression by
Integrins are expressed by virtually all cells and play key roles in a range of cellular processes. Changes in the integrin surface repertoire provide a means of altering the strength and ligand preferences of cell adhesion. Recent research has examined the affinity modulation of integrins, a rapid …
マウス・モノクローナル抗体 ab33171 交差種: Hu,Bb 適用: WB,IP,IHC-Fr,Flow Cyt…Integrin beta 3抗体一覧…画像、プロトコール、文献などWeb上の情報が満載のアブカムの Antibody…
Integrins are proteins important for integrating signals from cell to cell and for interpreting cues from their microenvironment that greatly influencing cell b...
高い抗原親和性、特異性と安定した品質を兼ね備えたアブカムのウサギ・モノクローナル抗体 RabMAb® ab52971 交差種: Hu 適用: WB,IHC-P,IHC-Fr
Given its effects on cell spreading and motility, Rap1 was postulated some time ago to be involved in integrin function; however, confirmation of this required alternative cellular models. Leukocytes represented such a model, since the positive effect of GTP-bound Rap1 on integrin-mediated adhesion is easier to see: in blood cells, in contrast to the more traditional, fibroblast and epithelial, adherent cell systems, integrins are normally kept inactive. Upon inside-out signalling elicited by various agonists, leukocyte integrins can rapidly and transiently be converted to a functionally active, ligand-binding state able to trigger the classic outside-in signalling to Rho-like GTPases (Harris et al., 2000; Schoenwaelder and Burridge, 1999).. Rap1 regulates functional activation of several integrin heterodimers:α 4β1 (VLA-4), α5β1 (VLA-5), αLβ2 (LFA-1, CD11a/CD18), αMβ2 (CR3, CD11b/CD18) and αIIbβ3 (Reedquist et al., 2000; Caron et al., 2000; Katagiri et al., 2000; Arai et al., 2001; ...
We have provided evidence that MK specifically binds to α4β1- and α6β1-integrins. The ligands of α4β1-integrin are fibronectin, a major component of the ECM, and VCAM, a member of the immunoglobulin superfamily. α4β1-Integrin recognizes LDV in the alternatively spliced III CS domain of fibronectin (Guan and Hynes, 1990; Kleiman and Mosher, 2002). α4β1-Integrin plays important roles in cell migration - it governs lymphocyte migration (Rose et al., 2001), is involved in recruitment of neutrophils to inflammatory sites (Burns et al., 2001; Henderson et al., 2001) and is essential for the migration of epicardial progenitor cells to the surface of the heart to form the epicardium (Sengbusch et al., 2002). MK also promotes the migration of neutrophils, macrophages, UMR-106 osteoblastic cells and neurons (Takada et al., 1997; Maeda et al., 1999; Horiba et al., 2000; Qi et al., 2001). Therefore, we postulated that MK-induced migration of UMR-106 cells might be mediated by α4β1-integrin. ...
The integrin family of cell surface receptors is evolutionary conserved and found in all multicellular animals. In humans 8-alpha and 18-beta integrins are non-covalently associated into 24 dimers. Integrins mediate cell-extracellular matrix and cell-cell interactions and participate in cell signalling. This ideally places integrins to regulate vital processes such as cell adhesion, migration, differentiation and cytoskeleton dynamics. Integrins also play a fundamental role in regulating cell survival and anoikis. In this thesis molecular mechanisms employed by integrins to induce signal transduction, independently or through crosstalk with other receptors, were characterised.. Rictor-mTOR (mTORC2) was required for Akt Ser473 phosphorylation in response to β1 integrin-mediated adhesion as well as EGF-, PDGF- or LPA-stimulation of MCF7 cells. ILK and PAK were dispensable for Akt Ser473 phosphorylation upon β1 integrin-engagement or EGF-stimulation. PAK was needed when this phosphorylation was ...
Mutations in the genes KRIT1, CCM2, and PDCD10 are known to result in the formation of cerebral cavernous malformations (CCMs). Although these genes have been known to be associated with CCMs since the 1990s, numerous discoveries have been made that better elucidate how they and their subsequent protein products are involved in CCM pathogenesis. Since our last review of the molecular genetics of CCM pathogenesis in 2012, breakthroughs include a more thorough understanding of the protein structures of the gene products, involvement with integrin proteins, and MEKK3 signaling pathways, and the importance of CCM2-PDCD10 interactions 1). Programmed cell death protein 10 is a protein that in humans is encoded by the PDCD10 gene. This gene encodes a protein, originally identified in a premyeloid cell line, with similarity to proteins that participate in apoptosis. Three alternative transcripts encoding the same protein, differing only in their 5 UTRs, have been identified for this gene. Loss of ...
Although ACE2 (angiotensin converting enzyme 2) is considered the primary receptor for CoV-2 cell entry, recent reports suggest that alternative pathways may contribute. This paper considers the hypothesis that viral binding to cell-surface integrins may contribute to the high infectivity and widespread extra-pulmonary impacts of the SARS-CoV-2 virus. This potential is suggested on the basis of the emergence of an RGD (arginine-glycine-aspartate) sequence in the receptor-binding domain of the spike protein. RGD is a motif commonly used by viruses to bind cell-surface integrins. Numerous signaling pathways are mediated by integrins and virion binding could lead to dysregulation of these pathways, with consequent tissue damage. Integrins on the surfaces of pneumocytes, endothelial cells and platelets may be vulnerable to CoV-2 virion binding. For instance, binding of intact virions to integrins on alveolar cells could enhance viral entry. Binding of virions to integrins on endothelial cells could ...
Integrins are transmembrane receptors that facilitate cell-extracellular matrix (ECM) adhesion. Upon ligand binding, integrins activate signal transduction pathways that mediate cellular signals such as regulation of the cell cycle, organization of the intracellular cytoskeleton, and movement of new receptors to the cell membrane. The presence of integrins allow rapid and flexible responses to events at the cell surface. Several types of integrins exist, and one cell may have multiple different types on its surface. Integrins are found in all animals.
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Shop a large selection of products and learn more about CD29 (Integrin beta 1) Mouse anti-Human, PE, Clone: TS2/16, eBioscience 25 Tests; PE.
References for Abcams Integrin beta 5 peptide (786-799) (ab45697). Please let us know if you have used this product in your publication
These data show that high levels of αvβ6 integrins are significantly associated with poor prognosis. There is also a trend towards worse prognosis with high levels of αSMA. However, there were no differences observed between samples with UIP or NSIP histology, although this study was not sufficiently powered to detect a difference between the two groups. Furthermore, there was no apparent relationship between the number of fibroblastic foci and mortality, consistent with previous reports [8]. This is the first study to demonstrate a tissue immunomarker in ILD with a significant association with the prognosis. A notable observation is that the median survival of patients with the highest expression of the αvβ6 integrin was only 25 months, which is comparable to the published survival data in IPF. This suggests that increased expression of the αvβ6 integrin may represent a distinct endotype of progressive fibrotic ILD, and could be useful as a biomarker for disease progression and ...
The activation of integrin-type adhesion receptors might result in the increased affinity of the receptor for ligand. In addition, the activated receptor might display new epitopes, which are increasingly monitored in clinical settings. Here, we highlight examples of integrin activation that is no …
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The regulation of CD11b integrin levels on human blood leukocytes and leukotriene B4-stimulated skin by a specific leukotriene B4 receptor antagonist (LY293111 ...
alpha 4 integrins are cell surface receptors that mediate cell-extracellular matrix (ECM) and cell-cell adhesions by interacting with fibronectin (FN) and vascular cell adhesion molecule 1 (VCAM-1), respectively. We have generated a null mutation in the gene for the alpha 4 integrin subunit. Homozygous null embryos express no alpha 4 integrins and show two unexpected defects, both of which lead to embryonic lethality. The first defect is failure of fusion of the allantois with the chorion during placentation. The second is in the development of the epicardium and coronary vessels leading to cardiac hemorrhage. Both processes clearly involve alpha 4 integrin interactions that were previously unsuspected. alpha 4 integrin and VCAM-1 are expressed at the sites of these interactions. These results raise the possibility of abortifacients targeting alpha 4 integrins, and raise serious questions about potential side effects of drugs currently being designed to block alpha 4 integrin functions in ...
The mammary gland epithelium comprises two major cell types: basal and luminal. Basal cells interact directly with the extracellular matrix (ECM) and express higher levels of the ECM receptors, integrins, than luminal cells. We show that deletion of beta1 integrin from basal cells abolishes the regenerative potential of the mammary epithelium and affects mammary gland development. The mutant epithelium was characterized by an abnormal ductal branching pattern and aberrant morphogenesis in pregnancy, although at the end of gestation, the secretory alveoli developed from beta1 integrin-positive progenitors. Lack of beta1 integrin altered the orientation of the basal-cell division axis and in mutant epithelium, in contrast to control tissue, the progeny of beta1 integrin-null basal cells, identified by a genetic marker, was found in the luminal compartment. These results reveal, for the first time, the essential role of the basal mammary epithelial cell-ECM interactions mediated by beta1 integrins in the
Integrin beta 1 / CD29 antibody [HM beta 1-1] (integrin beta 1 (fibronectin receptor beta)) for FACS, IP. Anti-Integrin beta 1 / CD29 mAb (GTX42117) is tested in Mouse, Rat samples. 100% Ab-Assurance.
Autor: Pozzi, A. et al.; Genre: Zeitschriftenartikel; Im Druck veröffentlicht: 2008-04-15; Keywords: kidney; development; basement membrane; receptors; Titel: beta 1 integrin expression by podocytes is required to maintain glomerular structural integrity
Invitrogen Anti-CD61 (Integrin beta 3) Monoclonal (2C9.G3), eBioscience™, Catalog # 16-0611-82. Tested in Flow Cytometry (Flow), Neutralization (Neu) and Functional assay (FN) applications. This antibody reacts with Mouse, Rat samples. Supplied as 100 µg purified antibody (1 mg/mL).
Invitrogen Anti-CD61 (Integrin beta 3) Monoclonal (2C9.G3), eBioscience™, Catalog # 11-0611-82. Tested in Flow Cytometry (Flow) applications. This antibody reacts with Mouse, Rat samples. Supplied as 100 µg purified antibody (0.5 mg/mL). Pengcheng Zhou, Sonia Erfani, Zeyi Liu, Changhe Jia, Yecang Chen, Bingwei Xu, Xinyu Deng, Jose E. Alfáro, Li Chen, Dana Napier, Michael Lu, Jian-An Huang,...
CD49d, also known as α4 integrin or VLA-4 α chain, is an integrin chain that forms a heterodimer with either integrin β1 (α4β1, VLA-4) or β7 (α4β7). It binds to VCAM-1, MAdCAM-1, fibronectin, and collagens.
Monoklonale und polyklonale Integrin beta 4 Antikörper für viele Methoden. Ausgesuchte Qualitäts-Hersteller für Integrin beta 4 Antikörper. Hier bestellen.
Human Keratinocyte Stem Cell Serum Free Differentiation Media.. This product is also available with Serum Cat# M36008-09DS. This product would require pre-coated flasks with Human Keratinocyte Stem Cell Extra-cellular Differentiation Matrix Cat# D36008-09 and Human Keratinocyte Stem Cells Cat# 36008-09. This product is tissue tested including Stem Cells and is available as 500ml sterile filtered unit.. The product is also available as a pack of 6, 500ml unit sizes.. ...
TY - JOUR. T1 - p63 identifies keratinocyte stem cells. AU - Pellegrini, Graziella. AU - Dellambra, Elena. AU - Golisano, Osvaldo. AU - Martinelli, Enrica. AU - Fantozzi, Ivana. AU - Bondanza, Sergio. AU - Ponzin, Diego. AU - McKeon, Frank. AU - De Luca, Michele. PY - 2001/3/13. Y1 - 2001/3/13. N2 - The proliferative compartment of stratified squamous epithelia consists of stem and transient amplifying (TA) keratinocytes. Some polypeptides are more abundant in putative epidermal stem cells than in TA cells, but no polypeptide confined to the stem cells has yet been identified. Here we show that the p63 transcription factor, a p53 homologue essential for regenerative proliferation in epithelial development, distinguishes human keratinocyte stem cells from their TA progeny. Within the cornea, nuclear p63 is expressed by the basal cells of the limbal epithelium, but not by TA cells covering the corneal surface. Human keratinocyte stem and TA cells when isolated in culture give rise to holoclones ...
Rat Keratinocyte Stem Cells Frozen Vial. This Product is also available as Plated Cells. T25 Plated Cells: $550.00. T75 Plated Cells: $750.00. T150 Plated Cells: $2500.00. T225 Plated Cells: $5500.00. This product also requires Celprogens Rat Keratinocyte Stem Cell Culture Extra-cellular Matrix. Cat# E55008-09 and Complete Media with Serum Cat# M55008-09S, and for serum free conditions Cat# M55008-09 is required provided the cells are weaned off the serum. as indicated in their specified protocol provided with purchase of these cells.. Source : Rat Skin. Positive Markers: Keratinocyte function (CD44), and profileration index (Ki67), keratin, CD71, CD34, keratinocyte derived chemokine (KC).. 120 Population Doublings.. Cells are only guaranteed with purchase of Celprogen Media, Extra Cellular Matrix, Trypsin EDTA, 1X PBS, and freezing media for the appropriate cell culture, for 30 days from the date of shipment.. ...
Epithelial ovarian cancer is a fatal disease, with a cure rate of only 30%. Several recent studies have targeted integrins for cancer treatment. Preclinical studies have shown the effectiveness of several integrin inhibitors for blocking cancer progression, especially by blocking angiogenesis. Because the initial critical step in ovarian cancer metastasis is the attachment of cancer cells to the peritoneum or omentum and because clinical trials have provided positive results for anti-angiogenic therapy, therapies targeting integrins may be the most feasible approach for treating cancer. This review summarizes the current understanding of integrin biology in ovarian cancer metastasis and various therapeutic approaches involving integrin inhibitors. However, no integrin inhibitor has shown favorable results thus far. However, conjugates of cytotoxic agents with the triplet sequence arginine-glycine-aspartate (RGD) peptides targeting α5β1-, αvβ3-, and αvβ6-integrins may be promising integrin
Integrins are cell adhesion receptors which mediate interactions between the extracellular matrix and the actin cytoskeleton. They are heterodimers composed of α and β subunits. As adhesion receptors, integrins are important for cell-cell and cell-matrix interactions and therefore are essential for the structural integrity of an organ. Moreover, integrin-extracellular matrix interactions play important roles in the coordinated integration of external and internal cues that are essential for proper development. β1 integrin is the most widely expressed integrin and controls various developmental processes, including neurogenesis, chondrogenesis, skin and hair follicle morphogenesis, and myoblast fusion. To determine the role of β1 integrin in normal development of the mouse mammary gland, with a particular emphasis on how β1 integrins influcence proliferation, differentiation and apoptosis; we examined the consequence of conditional deletion of β1 integrin in mammary epithelia. ...
The Shc adaptor protein, particularly its p52 isoform, has been identified as a primary signaling partner for the tyrosine(s)-phosphorylated cytoplasmic tails of activated β3 integrins. Inspired by our recent structure of the Shc PTB domain in complex with a bi-phosphorylated peptide derived from β3 cytoplasmic tail, we have initiated the investigation of Shc interaction with phospholipids of the membrane. We are particularly focused on PtdIns and their effects on Shc mediated integrin signaling in vitro. Here we present thermodynamic profiles and molecular details of the interactions between Shc, integrin, and PtdIns, all of which have been studied by ITC and solution NMR methods. A model of p52 Shc interaction with phosphorylated β3 integrin cytoplasmic tail at the cytosolic face of the plasma membrane is proposed based on these data.
Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with ...
Order monoclonal and polyclonal Integrin beta 4 antibodies for many applications. Selected quality suppliers for anti-Integrin beta 4 antibodies.
Mouse Monoclonal Anti-Integrin beta 4/CD104 Antibody (UM-A9) [DyLight 755]. Validated: WB, ELISA, Flow, ICC/IF, IHC-Fr. Tested Reactivity: Human. 100% Guaranteed.
Dr. Vaclav Vetvicka, PhD is the Worlds Leading Expert on Beta Glucan and has studied extensively how it affects your Immune System.
... antigens, cd27 MeSH D23.050.301.264.035.128 - antigens, cd28 MeSH D23.050.301.264.035.129 - antigens, cd29 MeSH D23.050.301.264 ... antigens, cd20 MeSH D23.050.301.264.051.140 - antigens, cd40 MeSH D23.050.301.264.129 - antigens, cd29 MeSH D23.050.301.264.894 ... antigens, cd27 MeSH D23. - antigens, cd28 MeSH D23. - antigens, cd29 MeSH D23. - ... antigens, cd29 MeSH D23.101.100.150 - antigens, differentiation, b-lymphocyte MeSH D23. - antigens, cd5 MeSH ...
... antigens, cd18 MeSH D12.776.543.750.705.408.200.500 - antigens, cd29 MeSH D12.776.543.750.705.408.200.750 - integrin beta3 MeSH ... antigen, b-cell MeSH D12.776.543.750.705.816.821.500 - antigens, cd79 MeSH D12.776.543.750.705.816.824 - receptors, antigen, t- ... antigens, cd22 MeSH D12.776.543.550.200.124 - antigens, cd24 MeSH D12.776.543.550.200.131 - antigens, cd31 MeSH D12.776.543.550 ... antigens, cd27 MeSH D12.776.543.750.705.852.760.072 - antigens, cd30 MeSH D12.776.543.750.705.852.760.097 - antigens, cd40 MeSH ...
CD29, or CD79a. The cells may also express many of the structural and non-structural gene abnormalities cited in the ... or one of the various tests for hepatitis C antigen. Extracavitary PEL is diagnosed based on findings that their mass lesions ... with PEL that is associated with cirrosis due to hepatitis evidence positive serum tests for the hepatitis virus B antigen ( ...
CK19, Cytokeratin 19, K19) Kit L-selectin (CD62L) Lamin A/C Lewis X antigen (Le(X)) LeX Lgr5 Lrp4 MCM2 MCSP Metallothionein (MT ... CD29) TNFRSF8 (CD30) PECAM-1 (CD31) Siglec-3 (CD33) CD34 CD44 NCAM (CD56) CD73 CD9 CD90 CDCP1 Circulating anticoagulants ... May 2006). "Lack of expression of the chondroitin sulphate proteoglycan neuron-glial antigen 2 on candidate stem cell ... Muramatsu T, Muramatsu H (2004). "Carbohydrate antigens expressed on stem cells and early embryonic cells". Glycoconjugate ...
Macrophage-1 antigen (CD11b+CD18). *VLA-4 (CD49d+CD29). *Glycoprotein IIb/IIIa (ITGA2B+ITGB3) ...
Macrophage-1 antigen (CD11b+CD18). *VLA-4 (CD49d+CD29). *Glycoprotein IIb/IIIa (ITGA2B+ITGB3) ... "Interaction of glycogen synthase kinase 3beta with the DF3/MUC1 carcinoma-associated antigen and beta-catenin". Molecular and ...
Macrophage-1 antigen (CD11b+CD18). *VLA-4 (CD49d+CD29). *Glycoprotein IIb/IIIa (ITGA2B+ITGB3) ... Primarily, the VCAM-1 protein is an endothelial ligand for VLA-4 (Very Late Antigen-4 or integrin α4β1) of the β1 subfamily of ...
Macrophage-1 antigen (CD11b+CD18). *VLA-4 (CD49d+CD29). *Glycoprotein IIb/IIIa (ITGA2B+ITGB3) ... In humans, the CD44 antigen is encoded by the CD44 gene on Chromosome 11.[5] CD44 has been referred to as HCAM (homing cell ... The CD44 antigen is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. ... Indian blood group system at BGMUT Blood Group Antigen Gene Mutation Database at NCBI, NIH ...
Very late antigen receptor:. *Integrin alpha1beta1. *Integrin alpha2beta1. *Integrin alpha3beta1. *VLA-4 *CD49d+CD29 ... "Entrez Gene: ITGB3 integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)".. *^ May, K. E.; Villar, J.; Kirtley, S.; ... CD61+Antigens at the US National Library of Medicine Medical Subject Headings (MeSH) ...
Tissue Antigens (англ.)русск. : journal. - 2007. - Vol. 68, no. 6. - P. 509-517. - DOI:10.1111/j.1399-0039.2006.00726.x. - PMID ...
1997). "The Oka blood group antigen is a marker for the M6 leukocyte activation antigen, the human homolog of OX-47 antigen, ... 1992). "Human leukocyte activation antigen M6, a member of the Ig superfamily, is the species homologue of rat OX-47, mouse ... Kasinrerk W, Fiebiger E, Stefanová I, Baumruker T, Knapp W, Stockinger H (1992). "Human leukocyte activation antigen M6, a ... Ok blood group system at BGMUT Blood Group Antigen Gene Mutation Database at NCBI, NIH ...
CD97 antigen je protein koji je kod ljudi kodiran CD97 genom.[1][2][3] ... CD29 • CD30 • CD31 • CD32 (A, B) • CD33 • CD34 • CD35 • CD36 • CD37 • CD38 • CD39 • CD40 • CD41 • CD42 (a, b, c, d) • CD43 • ... 2001). „Tissue distribution of the human CD97 EGF-TM7 receptor". Tissue Antigens. 57 (4): 325-31. PMID 11380941. doi:10.1034/j. ... Expression cloning and chromosomal mapping of the leukocyte activation antigen CD97, a new seven-span transmembrane molecule of ...
CD74 (англ. HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain) - мембранный белок ... II histocompatibility antigen gamma chaingamma chain of class II antigensIiHLA-DR antigens-associated invariant chainIa antigen ... Riberdy J.M., Newcomb J.R., Surman M.J., Barbosa J.A., Cresswell P. HLA-DR molecules from an antigen-processing mutant cell ... Machamer C.E., Cresswell P. Biosynthesis and glycosylation of the invariant chain associated with HLA-DR antigens (англ.) // ...
Seligman P. A., Butler C. D., Massey E. J., etal. The p97 antigen is mapped to the q24-qter region of chromosome 3; the same ... Le Beau M. M., Diaz M. O., Plowman G. D., etal. Chromosomal sublocalization of the human p97 melanoma antigen. (англ.) // Hum. ... Plowman G. D., Brown J. P., Enns C. A., etal. Assignment of the gene for human melanoma-associated antigen p97 to chromosome 3 ... Rose T. M., Plowman G. D., Teplow D. B., etal. Primary structure of the human melanoma-associated antigen p97 ( ...
... uveitis antigens induce CXCR3- and CXCR5-expressing lymphocytes and immature dendritic cells to migrate (англ.) // Blood (англ ...
... is a co-receptor of the T cell receptor (TCR) and assists the latter in communicating with antigen-presenting cells. The ... Leucocyte typing: human leucocyte differentiation antigens detected by monoclonal antibodies: specification, classification, ... T cells displaying CD4 molecules (and not CD8) on their surface, therefore, are specific for antigens presented by MHC II and ... CD1+Antigen at the US National Library of Medicine Medical Subject Headings (MeSH) ...
CD29 • CD30 • CD31 • CD32 (A, B) • CD33 • CD34 • CD35 • CD36 • CD37 • CD38 • CD39 • CD40 • CD41 • CD42 (a, b, c, d) • CD43 • ... 1991). „Expression of the YB5.B8 antigen (c-kit proto-oncogene product) in normal human bone marrow". Blood. 78 (1): 30-7. PMID ... 2003). „Signal transduction-associated and cell activation-linked antigens expressed in human mast cells". Int. J. Hematol. 75 ...
In addition to aiding with cytotoxic T cell antigen interactions the CD8 co-receptor also plays a role in T cell signaling. The ... the CD8 co-receptor plays a role in T cell signaling and aiding with cytotoxic T cell antigen interactions. ... This affinity keeps the T cell receptor of the cytotoxic T cell and the target cell bound closely together during antigen- ... Once the T cell receptor binds its specific antigen Lck phosphorylates the cytoplasmic CD3 and ζ-chains of the TCR complex ...
Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) also known as CD66e (Cluster of Differentiation 66e), is a ... 2001). "Heterogeneous RNA-binding protein M4 is a receptor for carcinoembryonic antigen in Kupffer cells". J. Biol. Chem. 276 ( ... CEACAM5, CD66e, CEA, carcinoembryonic antigen related cell adhesion molecule 5. External IDs. HomoloGene: 128801 GeneCards: ... Oikawa S, Nakazato H, Kosaki G (1987). "Primary structure of human carcinoembryonic antigen (CEA) deduced from cDNA sequence". ...
van Rhenen A., van Dongen G. A., Kelder A., et al. The novel AML stem cell associated antigen CLL-1 aids in discrimination ...
CD29 • CD30 • CD31 • CD32 (A, B) • CD33 • CD34 • CD35 • CD36 • CD37 • CD38 • CD39 • CD40 • CD41 • CD42 (a, b, c, d) • CD43 • ... 1996). "CD88 antibodies specifically bind to C5aR on dermal CD117+ and CD14+ cells and react with a desmosomal antigen in human ...
A new ligand for human leukocyte antigen class II antigens". The Journal of Experimental Medicine. 176 (2): 327-37. doi:10.1084 ... A new ligand for human leukocyte antigen class II antigens". The Journal of Experimental Medicine. 176 (2): 327-37. doi:10.1084 ... antigen processing and presentation of exogenous peptide antigen via MHC class II. ... antigen binding. • transmembrane signaling receptor activity. • MHC class II protein binding. Cellular component. • membrane. • ...
It is also called Lewis x and SSEA-1 (stage-specific embryonic antigen 1) and represents a marker for murine pluripotent stem ... CD15 Antigen at the US National Library of Medicine Medical Subject Headings (MeSH) ... CD15 (3-fucosyl-N-acetyl-lactosamine) is a cluster of differentiation antigen - an immunologically significant molecule. CD15 ...
B cells can present antigens to a specialized group of helper T cells called TFH cells. If an activated TFH cell recognizes the ... Roles of T cell-B-cell-activating molecule (5c8 antigen) and CD40 in contact-dependent help". Journal of Immunology. 149 (12): ... It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In ... Grewal, IS; Xu, J; Flavell, RA (7 December 1995). "Impairment of antigen-specific T-cell priming in mice lacking CD40 ligand". ...
I. Partial characterization of soluble Ki-1 antigen and detection of the antigen in cell culture supernatants and in serum by ... Josimovic-Alasevic O, Dürkop H, Schwarting R, Backé E, Stein H, Diamantstein T (Jan 1989). "Ki-1 (CD30) antigen is released by ... CD30+Antigens at the US National Library of Medicine Medical Subject Headings (MeSH) ... results from cDNA cloning and sequence comparison of the CD30 antigen from different sources". Molecular Immunology. 31 (17): ...
Eichler W, Hamann J, Aust G (Nov 1997). "Expression characteristics of the human CD97 antigen". Tissue Antigens. 50 (5): 429-38 ... Hamann J, Wishaupt JO, van Lier RA, Smeets TJ, Breedveld FC, Tak PP (Apr 1999). "Expression of the activation antigen CD97 and ... Tissue Antigens. 57 (4): 325-31. doi:10.1034/j.1399-0039.2001.057004325.x. PMID 11380941.. ... "Expression cloning and chromosomal mapping of the leukocyte activation antigen CD97, a new seven-span transmembrane molecule of ...
"Direct association of adenosine deaminase with a T cell activation antigen, CD26". Science. 261 (5120): 466-9. doi:10.1126/ ...
CD64+Antigens at the US National Library of Medicine Medical Subject Headings (MeSH) ...
CD29 • CD30 • CD31 • CD32 (A, B) • CD33 • CD34 • CD35 • CD36 • CD37 • CD38 • CD39 • CD40 • CD41 • CD42 (a, b, c, d) • CD43 • ... 2000). "Characterization of a new member of the TNF family expressed on antigen presenting cells.". Biol. Chem. 380 (12): 1443- ... "BLyS receptor signatures resolve homeostatically independent compartments among naïve and antigen-experienced B cells.". Semin ...
Ebert LM, McColl SR (2002). "Up-regulation of CCR5 and CCR6 on distinct subpopulations of antigen-activated CD4+ T lymphocytes ... This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may ... dendritic cells induce antitumor immunity when genetically fused with nonimmunogenic tumor antigens". J. Immunol. 167 (11): ...
Macrophage-1 antigen (CD11b+CD18). *VLA-4 (CD49d+CD29). *Glycoprotein IIb/IIIa (ITGA2B+ITGB3) ...
CD29 • CD30 • CD31 • CD32 (A, B) • CD33 • CD34 • CD35 • CD36 • CD37 • CD38 • CD39 • CD40 • CD41 • CD42 (a, b, c, d) • CD43 • ... CD97 antigen je protein koji je kod ljudi kodiran CD97 genom.[1][2][3] ... 2001). "Tissue distribution of the human CD97 EGF-TM7 receptor". Tissue Antigens 57 (4): 325-31. PMID 11380941. doi:10.1034/j. ... "Expression cloning and chromosomal mapping of the leukocyte activation antigen CD97, a new seven-span transmembrane molecule of ...
antigen processing and presentation of peptide antigen via MHC class I. • antigen processing and presentation of exogenous ... antigen processing and presentation of exogenous peptide antigen via MHC class I. • lipoprotein transport. • negative ... peptide antigen via MHC class I, TAP-dependent. • platelet degranulation. • MyD88-dependent toll-like receptor signaling ...
antigen binding. • virus receptor activity. • protein binding. • transmembrane signaling receptor activity. • identical protein ...
T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell. • T cell antigen ... CD29 · CD30 · CD31 · CD32 (A, B) · CD33 · CD34 · CD35 · CD36 · CD37 · CD38 · CD39 · CD40 · CD41 · CD42 (a, b, c, d) · CD43 · ...
... antigens cd29 include Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples ... Antigens, CD29: Integrin beta-1 chains which are expressed as heterodimers that are noncovalently associated with specific ... Cd29 is expressed on resting and activated leukocytes and is a marker for all of the very late activation antigens on cells. ( ... from: Barclay et al., The Leukocyte Antigen FactsBook, 1993, p164) Isolation and Characterization of Cardiac Mesenchymal ...
CD29" by people in this website by year, and whether "Antigens, CD29" was a major or minor topic of these publications. ... "Antigens, CD29" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... Below are the most recent publications written about "Antigens, CD29" by people in Profiles. ... Below are MeSH descriptors whose meaning is more general than "Antigens, CD29". ...
CD29 is a 130 kD protein, also known as integrin β1, VLA-β chain, or GPIIa. ... Antigen Details Structure Integrin family, 130 kD Distribution Leukocytes, endothelial cells, smooth muscle, epithelial cells ... CD29 is a 130 kD protein, also known as integrin β1, VLA-β chain, or GPIIa. It is a member of the integrin family, expressed ... Antigen References 1. Noto K, et al. 1995. Int. Immunol. 7:835.. 2. Springer TA. 1990. Nature 346:425. ...
CD29 is a 130 kD single chain type I glycoprotein also known as integrin β1, VLA-β chain, or gpIIa. ... Antigen Details Structure Integrin, type I glycoprotein, forms VLA-1 to VLA-6 heterodimers with CD49a-f (α1-α6), also ... Integrins, which include CD29, bind to several cell surface (e.g. VCAM-1, MadCAM-1) and extracellular matrix molecules. CD29 ... CD29 is a 130 kD single chain type I glycoprotein also known as integrin β1, VLA-β chain, or gpIIa. It is broadly expressed on ...
CD29 antibody [12G10]. Validated in WB, ICC/IF, IHC-Fr, FACS, IP, ELISA. Tested in Human, Mink. - professional antibody ... Antigen Species Human Immunogen Purified human beta1 integrin preparation from HT1080 fibrosarcoma cell extract. ... There are currently no reviews for Integrin beta 1 / CD29 antibody [12G10] (GTX75707). Be the first to share your experience ... There are currently no references for Integrin beta 1 / CD29 antibody [12G10] (GTX75707). Be the first to share your ...
Shop a large selection of products and learn more about CD29 (Integrin beta 1) Armenian Hamster anti-Mouse, Rat, Clone: ... Antigen. CD29 (Integrin beta 1). Classification. Monoclonal. Concentration. 0.5 mg/mL. Formulation. PBS with 0.09% ... CD29 binds non-covalently with the alpha integrins CD49a-f to form the VLA-1 through VLA-6 complexes, as well as with CD51. ... CD29 (beta1 integrin subunit, GPIIa) forms non-covalently linked heterodimers with at least 6 different alpha chains (alpha1- ...
... products and learn more about CD29 Mouse anti-Human, Brilliant Violet 786, Clone: MAR4., BD 100 Tests; 100 Tests; Brilliant ... comprising the very late activation antigen (VLA) subfamily of adhesion receptors. The β 1 subunit has a broad tissue ... The MAR4 monoclonal antibody specifically binds to CD29. CD29 is a 130 kDa integrin β 1 subunit that is expressed as a ...
CD_antigen: CD29. ,p>This subsection of the ,a href="">Names and ...
DETERMINATION OF GENE EXPRESSION OF CD25 AND CD29 IN ALBINO RATS IMMUNIZED WITH VI ANTIGEN OF SALMONELLA TYPHI COUPLED TO ... TT group (121.6±38.4) at (P≤ 0.05). The gene expression of CD25 and CD29 was done by RT-qPCR . The results of relative gene ... The results of relative gene expression in CD29 gene showed that T3group recorded significantly increment (162.3256±89.52) than ... to develop an experimental vaccine against typhoid fever through determination of the immunogenic properties of Vi antigen when ...
Mouse Monoclonal Anti-Integrin beta 1/CD29 Antibody (12G10) [Alexa Fluor® 700]. Stem Cell Marker. Validated: WB, ELISA, Flow, ... Alternate Names for Integrin beta 1/CD29 Antibody (12G10) [Alexa Fluor® 700]. *CD29 antigen ... Additional Integrin beta 1/CD29 Products. Integrin beta 1/CD29 NB100-63255AF700 * Integrin beta 1/CD29 Antibodies ... Home » Integrin beta 1/CD29 » Integrin beta 1/CD29 Antibodies » Integrin beta 1/CD29 Antibody (12G10) [Alexa Fluor® 700] ...
Antigens, CD29 / metabolism. Cell Line. Collagen Type I / pharmacology*. Enzyme Activation / drug effects. Enzyme Precursors / ... 0/Antigens, CD29; 0/Collagen Type I; 0/Enzyme Precursors; 0/Integrin alpha1; 127497-59-0/Tissue Inhibitor of Metalloproteinase- ...
0/Antibodies; 0/Antigens, CD29; 0/Chemokine CCL5; 0/Receptors, CCR5 From MEDLINE®/PubMed®, a database of the U.S. National ... Antigens, CD29 / metabolism. Cell Movement / physiology*. Chemokine CCL5 / genetics, pharmacology, physiology*. Endometrium / ...
Heat-stable antigen (HSA) CD14 CD29 β1 integrin CD144 VE-cadherin CD44 Hyaluronic acid/fibronectin receptor CD15 Stage-specific ... Lin antigens consist of the following group of lineage markers: CD2, CD3, CD4, CD5, CD8, NK1.1, B220, Ter-119, and Gr-1 ( ... Thymus cell antigen-1 (Thy-1) CD56 Neural cell adhesion molecule (NCAM) ... neuronal nuclear antigen; Ngn-3, neurogenin 3; Nkx2.5, NK2 homeobox 5; OCN, osteocalcin; OPN, osteopontin; Pax-6, paired box ...
Integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) antibody ... Noto K et al. Identification and functional characterization of mouse CD29 with a mAb. Int Immunol 7:835-42 (1995). PubMed: ...
Integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) antibody ... Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.. ... Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. ... Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. ...
integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes. MDF2, MSK12). ...
CD29 associates non‐covalently with the alpha integrins CD49a-f to form the VLA-1 through VLA-6, and CD51 to form αvß1 ... CD29 is broadly expressed on various tissues, including leukocytes, endothelial cells, and epithelial cells. Additional ... Clone REA1074 reacts with mouse and rat CD29 antigen, a 110-120 kDa integrin family member, also known as integrin ß1. ... Clone REA1074 reacts with mouse and rat CD29 antigen, a 110-120 kDa integrin family member, also known as integrin ß1. ...
The cytoplasmic domain of CD29 can interact with cytoskeletal proteins and signaling molecules. In humans, CD29 can be found in ... CD29 is also known as integrin β1 or GPIIa. It forms heterodimers with integrins α1-11 and αV to form functional integrin ... Clone REA1060 recognizes the human CD29 antigen, a 130 kDa single-pass type I membrane protein. ... Clone REA1060 recognizes the human CD29 antigen, a 130 kDa single-pass type I membrane protein. CD29 is also known as integrin ...
K., et al.: Identification and functional characterization of mouse CD29 with a mAb. Int. Immunol.7. 835-842 (1995). *. ... Publications] Nakajima,A.,et al.: Requirement of CD28-CD86 co-stimulation in the interaction between antigen-primed T helper ... Studies on the differention and activation mechanisms of T cell vi lymphocye functioning antigen (LFA).. Research Project ... Publications] Nakajima, A., et al.: Requirement of CD28-CD86 co-stimulation in the interaction between antigen-primed T helper ...
Sheep Polyclonal Anti-Bone marrow stromal cell antigen 1/CD157 Antibody. Validated: WB. Tested Reactivity: Mouse. 100% ... Additional Bone marrow stromal cell antigen 1/CD157 Products. Bone marrow stromal cell antigen 1/CD157 AF4710 * Bone marrow ... Blogs on Bone marrow stromal cell antigen 1/CD157. There are no specific blogs for Bone marrow stromal cell antigen 1/CD157, ... Home » Bone marrow stromal cell antigen 1/CD157 » Bone marrow stromal cell antigen 1/CD157 Antibodies » Bone marrow stromal ...
POSITIVE surface Antigen. NEGATIVE surface Antigen. Adhesion molecules. CD9 ; CD29 (β1-integrin);. CD49d (α4-integrin); CD54 ( ...
... antigen CD29 includes MDF2, MSK12)) for ELISA, FACS, ICC/IF, IHC, IP, WB, Functional Assay. Anti-Integrin beta 1 / CD29 mAb ( ... CD29 antibody [TS2/16] (integrin, beta 1 (fibronectin receptor, beta polypeptide, ... antigen CD29 includes MDF2, MSK12) Antibody , MDF2 Antibody , FNRB Antibody , CD29 Antibody , MSK12 Antibody. ... integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12). ...
Ki-67 Antigen (Ki 67 Antigen) 3. CD29 Antigens 4. Fibronectins (Fibronectin) ...
CD49f-CD29; Integrin alpha(6)beta(1); Integrin alpha6Abeta1; Integrin alpha6Bbeta1; Platelet Glycoprotein Ic-IIa; VLA-6; alpha ... Very Late Antigen Receptors: 8*Integrin alpha6beta1: 78. *Laminin Receptors: 415*Integrin alpha6beta1: 78 ... 6 beta 1 Integrin; alpha6beta1 Integrin; CD49f CD29; Glycoprotein Ic-IIa, Platelet; Ic-IIa, Platelet Glycoprotein; Integrin, ...
CD29 explanation free. What is CD29? Meaning of CD29 medical term. What does CD29 mean? ... Looking for online definition of CD29 in the Medical Dictionary? ... antigen CD29 includes MDF2, MSK12) (ITGB1), cyclin-dependent ... CD29. Also found in: Wikipedia. CD29. a type I transmembrane protein present on some CD4 helper T cells, platelets, and ... Previous studies have suggested CD29 is required for Ebola virus infection; blocking CD29 could halt Ebolas spread in the body ...
CD antigen CD29CD29• Fibronectin receptor subunit beta• FNRB• glycoprotein IIa• GPIIA• integrin beta 1• integrin beta-1• ... antigen CD29 includes MDF2, MSK12)• MDF2• MSK12• very late activation protein, beta polypeptide• VLA-4 subunit beta• VLAB• VLA- ...
integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) [Source. Mouse Orthologue:. Itgb1 ...
CD29, CD31, CD44, CD54, CD62e, CD106, CD166, and CD146). (e) Human leukocyte antigens (HLA-G) and costimulatory molecules (CD27 ... For instance, CD29 and CD166 were highly expressed whilst CD58, CD62, CD102, and CD146 were weakly expressed in all analyzed ... HLA antigens and costimulatory molecules profiling was performed to assess the immunogenicity state of liver-derived cells. As ... Profiling of cell adhesion molecules was also evaluated for CD29, CD44, CD49e, CD54, CD58, CD62, CD102, CD106, CD146, and CD166 ...
In phenotypic analysis using flow cytometry, similarity among all stem cell marker expression was found, excluding CD29 and ... In a phenotypic analysis using flow cytometry, similarity of stem cell marker expression was found excluding CD29 and CD105. In ... human leukocyte antigen HLA-DR is also negative (below 2%). Meanwhile, each cell type had positively expressed mesenchymal stem ... In phenotypic analysis using flow cytometry, similarity among all stem cell marker expression was found, excluding CD29 and ...
Alphabetical products and learn more about CD29 (Integrin beta 1) Rat anti-Mouse, Clone: KMI6, eBioscience™ 100 μg; ... Antigen. CD29 (Integrin beta 1). Classification. Monoclonal. Concentration. 0.5 mg/mL. Formulation. PBS with 0.09% ... CD29 (beta1 integrin subunit, GPIIa) forms non-covalently linked heterodimers with at least 6 different alpha chains (alpha1- ...
  • 7. A process as claimed in claim 1 wherein the tumor cells are from breast carcinoma, lung carcinoma, or neuroblastoma, and the antibody composition contains antibodies specific for antigens expressed on the surface of cells from breast carcinoma, lung carcinoma, or neuroblastoma. (
  • F ) Left: Confocal microscopy images of immune synapses of naïve B cells and LZ GC B cells on antigen-coated PLB stained with Alexa Fluor 488 phalloidin for F-actin (green) and antibodies specific for ezrin (red). (
  • Recently, we described a panel of monoclonal antibodies with superior selectivity for mesenchymal stem cells, including the monoclonal antibodies W8B2 against human mesenchymal stem cell antigen-1 (MSCA-1) and 39D5 against a CD56 epitope, which is not expressed on natural killer cells. (
  • Design and Methods Bone marrow derived mesenchymal stem cells from healthy donors were analyzed and isolated by flow cytometry using a large panel of antibodies against surface antigens including CD271, MSCA-1, and CD56. (
  • CD antigens for cluster of differentiation, which indicates a defined subset of cellular surface receptors (epitopes) that identify cell type and stage of differentiation, and which are recognized by antibodies. (
  • Antibodies to CD11a/CD18 (leukocyte function-associated antigen-1 [LFA-1]) present in ATG induced a dose-dependent down-modulation of cell surface expression of this beta2 integrin on lymphocytes, monocytes, and neutrophils. (
  • ATG also contained functional antibodies to the beta1 integrin CD49d/CD29 (VLA-4), the alpha4beta7 integrin, CD50, CD54, and CD102 but not to CD62L. (
  • Flow cytometric analysis revealed that pMSC expressed surface antigens also found on hMSC, including CD90, MSCA-1 (TNAP/W8B2 antigen), CD44, CD29 and SLA class I. Clonogenic outgrowth was significantly enriched following selection of CD271+ cells from BM of human and pig (129 ± 29 and 1961 ± 485 fold, respectively). (
  • Using immunocytochemistry we show that horse MS cells homogenously express collagens, alkaline phosphatase activity, CD44, CD90 and CD29. (
  • Besides, immunohistochemistry and flow cytometry revealed that SH2, SH3, CD71, CD29, CD44, CD90, and CD120A were all positive expressed, which can be generally used to identify and amplify the mesenchymal stem cells [ 1 ]. (
  • 11 , 12 Another population of CD34 + cells, located around the vasa vasorum in the adventitia of arteries and veins, also express typical pericyte markers (NG2, PDGFRβ, and RGS5) together with mesenchymal (CD44, CD90, CD73, CD29) and stemness antigens (Oct-4 and Sox-2). (
  • MHC class II molecules and CD29 were up-regulated in pro-Th1 DCs whilst CD18 and CD44 were up-regulated in pro-Th2 DCs. (
  • CD29 is a 130 kD protein, also known as integrin β 1 , VLA-β chain, or GPIIa. (
  • CD29 (beta1 integrin subunit, GPIIa) forms non-covalently linked heterodimers with at least 6 different alpha chains (alpha1-alpha6, CD49a-f) determining the binding properties of beta1 (VLA) integrins. (
  • CD29 is also known as integrin β1 or GPIIa. (
  • It was initially characterized independently as protein gpIIa appearing on platelets, as the common Beta subunit of the very late activation antigen (VLA), and as a component of various protein complexes binding to extracellular matrix proteins. (
  • CD49b non-covalently pairs with the β-1 integrin (CD29, GPIIa) to form the VLA-2 (very late activation antigen) complex. (
  • CD29 is also known as platelet GPIIa. (
  • CD29 (β1 integrin subunit, GPIIa) forms non-covalently linked heterodimers with at least 6 different α chains (α1-α6, CD49a-f) determining the binding properties of β1 (VLA) integrins. (
  • Then the section was stained with 10 µg/mL of CD3 (clone 1F4) Alexa Fluor® 488 (blue), 10 µg/mL of CD29 (clone HMβ1-1) Alexa Fluor® 647 (yellow), and 10 µg/mL of purified CD38 (clone 14.27) overnight at 4°C, followed by 5 µg/mL of anti-mouse IgG2b (clone RMG2b-1) Alexa Fluor® 594 (red) for two hours at room temperature. (
  • CD29 Mouse anti-Human, Brilliant Violet 786, Clone: MAR4. (
  • Clone REA1074 reacts with mouse and rat CD29 antigen, a 110-120 kDa integrin family member, also known as integrin ß1. (
  • Clone REA1060 recognizes the human CD29 antigen, a 130 kDa single-pass type I membrane protein. (
  • Hamster anti Mouse CD29 antibody, clone HM beta 1-1 recognizes the murine integrin beta 1 subunit (CD29), a ~110 kDa cell surface glycoprotein that is widely expressed by a variety of cells including all leucocytes. (
  • CD49d is the integrin α4 chain that non-covalently pairs with CD29 (β1 chain) to form the very late activation antigen 4 (VLA-4), or with the integrin β7 chain. (
  • In addition, lymphocytes respond to chemotactic stimuli, activate FBs via ICAM-1, and bind to non-cellular integrins expressed on collagen and fibronectin via surface VLA-1 (CD49a, CD29) and VLA-4 (CD49d, CD29). (
  • Integrins are a versatile family of adhesion molecules 2 of which α4β1 integrin (also called very-late antigen-4 or CD49d/CD29) is an important mediator of immune cell migration into the CNS. (
  • 2) MSCs must express the surface antigens CD105, CD73, and CD90. (
  • A lack of expression of hematopoietic antigens (CD45, CD34, CD14/CD11b, CD79a/CD19, HLA-DR) is recommended, along with a minimum purity of ≥95% for CD105, CD73, and CD90 positive cells and ≤2% expression of hematopoietic antigens. (
  • Dental stem cells express mesenchymal stem cell markers like Stro-1, CD146, CD106, CD90, CD73 CD29 and CD13. (
  • Integrin beta-1 (ITGB1), also named as CD29, is a 130 kDa single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. (
  • CD29 is a 130 kDa integrin β 1 subunit that is expressed as a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. (
  • 2001) Differential effects of CD18, CD29, and CD49 integrin subunit inhibition on neutrophil migration in pulmonary inflammation. (
  • That increase was independent of IFN-γ production by T cells, but was mediated by integrins CD11b, CD18, and CD29. (
  • Lymphocyte and monocyte ligands, L-selectin, sialated glycoproteins, lymphocyte function-associated antigen 1 (LFA-1) (CD11a, CD18), and Mac1 (CD11b, CD18) bind to the EC rec and modulate the migration of these cells. (
  • All integrins which include CD29 bind to extracellular matrix proteins including collagen, laminin, fibronectin and vitronectin, whereas some CD29-containing integrins can also interact with cellular receptors such as VCAM-1 and MadCAM-1. (
  • CD29 or the integrin Beta-1 belongs to the family of cell adhesion receptors. (
  • It associates with integrin β1 chain (or CD29) to form very late antigen-6, and with integrin β4 chain (or CD104) to form the α6β4 complex, both of which are important laminin receptors ( 12 ). (
  • Two of the company's founders, Drs. Michel Saelain and Renier J. Brentjens won the New York Intellectual Property Law Association's "Inventor of the Year" award for their work in the design of chimeric antigen receptors (CARs), a major part of the company's therapeutic platform. (
  • Non peptide antigen presentation to T-cell receptors on NKT cells. (
  • Since expression of CD antigens is associated with cellular differentiation, cancer cells may differ from their normal counterpart in their CD profile. (
  • They should express specific cell surface markers, such as cluster of differentiation (CD) 73, D90, CD105, and lack the expression of CD14, CD34, CD45 and human leukocyte antigen-DR (HLA-DR). (
  • These cells express both myeloid lineage differentiation antigen Gr-1 (Ly6G and Ly6C) and α M integrin CD11b, and in control mice represent ∼20% to 30% of normal bone marrow cells, 2% to 4% of all nucleated splenocytes, and are practically absent in lymph nodes. (
  • Dental tissues, a new source for stem cells, provide cells having mesenchymal stem cell characteristics such as fibroblast-like structure, expression of surface antigens specific for mesenchymal stem cells, regeneration ability, multilineage differentiation capacity and immunomodulatory features. (
  • Thymus cell antigen 1 (Thy1), also known as cluster of differentiation (CD)90, and integrin α6 (ITGA6), also known as CD49f, are important molecules in cancer and putative markers of various stem cell types. (
  • Thymus cell antigen 1 (Thy1), also known as cluster of differentiation (CD)90, is a 25-37-kDa glycophosphatidylinositol-anchored protein that is expressed in numerous cell types, including T cells, neurons, endothelial cells, fibroblasts and numerous tumor cells. (
  • The CD antigens / Cluster of differentiation nomenclature was established in the 1st International Workshop and Conference on Human Leukocyte Differentiation Antigens (HLDA), which was held in Paris in 1982. (
  • CD15, CD24, and CD29 define a surface biomarker code for neural lineage differentiation of stem cells. (
  • Fluorescence-activated cell sorting (FACS) for the CD15(-)/CD29(LO)/CD24(HI) profile reduced proliferative cell types in human embryonic stem cell differentiation. (
  • Description: The eBioHMb1-1 monoclonal antibody reacts with mouse and rat CD29 (integrin beta 1), a 110-120 kDa member of the beta integrin family expressed by leukocytes, endothelial, smooth muscle and epithelial cells. (
  • Description: The KMI6 monoclonal antibody reacts with mouse CD29 (integrin beta1), a 120-130kDa member of the beta integrin family expressed by leukocytes, endothelial, smooth muscle and epithelial cells. (
  • Stage-specific embryonic antigen (SSEA)-4, CD146 and stromal precursor antigen-1 (Stro-1) are the hallmarks of mesenchymal stem cells. (
  • Studies shown that the surface antigen phenotype of mesenchymal stem cells was not single but had the characteristics of mesenchymal, endothelial and muscle cells. (
  • Flow cytometry analysis of CD29 in peripheral blood granulocytes compared to an isotype control (blue). (
  • 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml, followed by the addition of 50 ul of isotype control and CD29 antibody [TS2/16] at a dilution of 0.5 ug/test. (
  • In contrast, CD14, CD79α and the embryonic stem cell markers Oct-4, SSEA (stage specific embryonic antigen) -1, -3, -4, TRA (tumor rejection antigen) -1-60 and -1-81 are not expressed. (
  • The HMß1-1 antibody reacts with both mouse and rat CD29. (
  • The antibody MEM-101A reacts with an extracellular epitope of CD29 (integrin beta1 chain), a 130 kDa single chain type I glycoprotein expressed as a heterodimer (non-covalently associated with the integrin alpha subunits 1-6). (
  • This antibody reacts with CD29 (integrin beta chain), a 130 KDa single chain type I glycoprotein expressed as a heterodimer (non-covalently associated with the integrin alpha subunits 1-6). (
  • The CD49b antigen is a transmembrane glycoprotein of 150 kDa also known as the α-2 integrin and as platelet GPIa. (
  • Intravascular large B-cell lymphoma cells have been demonstrated to lack cell surface proteins critical to lymphocyte transvascular migration, including CD29 ([beta]1 integrin) and CD54 (ICAM-1 or CD11a ligand). (
  • This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. (
  • Other molecules up-regulated in pro-Th2 DC compared to pro-Th1 DCs included some potentially involved in protein folding during antigen processing (clathrin and Rab-7), whilst other non-membrane proteins such as enzymes/transporters related to cell metabolism (malate dehydrogenase, pyruvate kinase, and ATPase Na+/K+) were also recorded. (
  • 9 ) involved the depletion of hematopoietic and endothelial cells from freshly dissociated cell preparations and definition of different mammary cell subpopulations based on their cell-surface expression of CD24 (heat-stable antigen) and either CD29 (β 1 -integrin) or CD49f (α 6 -integrin). (
  • Subsets that expressed highest levels of CD29 or CD49f and CD24 (termed the "double-positive" population) were found to be enriched in MaSCs by transplanting these cells at limiting dilution into cleared fat pads. (
  • and (3) CD15(-)/CD29(LO)/CD24(HI) selected neuroblasts and neurons. (
  • In conclusion, combinatorial CD15/CD24/CD29 marker profiles define neural lineage development of neural stem cell, neural crest, and neuronal populations from human stem cells. (
  • Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. (
  • The cytoplasmic domain of CD29 can interact with cytoskeletal proteins and signaling molecules. (
  • Staphylococcal enterotoxins are classified as superantigens that act by linking T-cell receptor with MHC class II molecules, which are expressed on classical antigen-presenting cells (APC). (
  • Staphylococcal enterotoxins are superantigens due its properties to induce extensive proliferation of T cells mediated by cross-linking of the variable region of the β chain of the T-cell receptor (TCR) with MHC class II molecules on antigen-presenting cells (APC) such as macrophages and dendritic cells ( Fraser, 2011 ). (
  • The CD antigens are protocol used for the identification and investigation of cell surface molecules providing targets for immunophenotyping of cells. (
  • OS2966 selectively modulates CD29 (integrin b1 subunit), a critical path driver of multiple mechanisms of tumor growth and progression including proliferation, invasion, angiogenesis, and therapy resistance. (
  • Tumor-induced T-cell abnormalities include antigen-specific nonresponsiveness (anergy/tolerance), deletion of T cells by apoptosis, and nonspecific suppression of T-cell function. (
  • In both animal models of cancer and in the clinical setting, unresponsiveness of T cells to tumor-specific antigens has been shown to be an early event in tumor progression. (
  • MDSCs may exert an immunosuppressive effect in both an antigen-specific and nonspecific manner depending on their localization and the specific characteristics of the tumor. (
  • It seems that in the tumor site, the immunosuppressive activity of MDSC is antigen-nonspecific and is primarily mediated by the production of nitric oxide (NO) in combination with a high arginase activity. (
  • Importantly, this antigen-specific nature of immune suppression may address an apparent paradox: why a number of studies have reported a lack of profound systemic immune suppression in tumor-bearing mice despite the presence of a very high number of immunosuppressive MDSC. (
  • CD antigens are used widely for research, immunotherary, tumor and drug target. (
  • Integrins, which include CD29, bind to several cell surface (e.g. (
  • CD29 binds non-covalently with the alpha integrins CD49a-f to form the VLA-1 through VLA-6 complexes, as well as with CD51. (
  • CD29 is broadly expressed on various tissues, including leukocytes, endothelial cells, and epithelial cells. (
  • CD29 is broadly expressed on majority of hematopoietic and non-hematopoietic cells (leukocytes, platelets, fibroblasts, endothelial cells, epithelial cells and mast cells). (
  • In situ staining demonstrated virus capsid antigen in koilocytotic cells and surrounding cells in the hyperplastic epithelial layer. (
  • The CD4,CD45RO, or memory T-cell, subset was numerically normal but expressed increased levels of adhesion markers (CD29, CD54, and CD58). (
  • CD157, also known as bone marrow stromal cell antigen 1 (BST-1), is a glycosyl phosphatidylinositol anchored membrane protein that belongs to the CD38 family (1). (
  • MA5-11429 targets CD29 in IHC (P) and Western blot applications and shows reactivity with Human and Rat samples. (
  • The antigen mediates cellular adhesion and has broad cellular reactivity, but is not expressed by erythrocytes. (
  • There are currently no images for Bone marrow stromal cell antigen 1/CD157 Antibody (AF4710). (
  • CD29 acts as a fibronectin receptor and is involved in a variety of cell-cell and cell-matrix interactions. (
  • Cluster designation (CD) antigens are cell surface markers that can be used to identify constituent cell populations of an organ. (
  • To date, the immunophenotyping of cell surface antigens relies on flow cytometry, allowing estimation of 3-6 markers at a time. (
  • Studies on the differention and activation mechanisms of T cell vi lymphocye functioning antigen (LFA). (
  • In phenotypic analysis using flow cytometry, similarity among all stem cell marker expression was found, excluding CD29 and CD105. (
  • With the exception of red blood cells and possible weak expression on granulocytes, CD29 is expressed nearly all cell and tissue types. (
  • Recent findings indicate that myeloid-derived suppressor cells can induce antigen-specific CD8 + T-cell tolerance through a posttranslation mechanism which involves modification (nitration) of CD8 and the T-cell receptor itself on the T-cell surface. (
  • Because MDSC are able to take up, process, and present antigens, this may provide a mechanistic foundation for antigen-specific T-cell suppression in cancer ( 5 , 6 ). (
  • B ) Kymographs of DIC images of naïve and LZ GC B cell immune synapses on antigen-containing PLB. (
  • CD29 complexes are involved in cell-cell and cell-matrix adhesion, depending on the α subunit associated to CD29. (
  • NOR MSC treatment of experimental type 1 diabetes resulted in long-term reversal of hyperglycemia, and therapy was shown to alter diabetogenic cytokine profile, to diminish T-cell effector frequency in the pancreatic lymph nodes, to alter antigen-presenting cell frequencies, and to augment the frequency of the plasmacytoid subset of DCs. (
  • However, no work has been done in horse MS cells to examine the expression profile of proteins and cell surface antigens that are expressed in human MS cells. (
  • These cells also expressed low levels of the stem cell antigen Sca-1 and seemed to have a basal position within the mammary epithelium ( 8 , 9 ). (
  • As a siganl, CD antigens usually initiated, altering the behavior of the cell. (
  • Expression levels of cell surface antigens such as CD38 and HLA-DR are related to HIV disease stages. (
  • The recently described DotScan antibody microarray technology enables the simultaneous analysis of a large number of cell surface antigens. (
  • Pairwise comparisons identified 17 statistically differential cell surface antigens including 5 novel ones (CD212b1, CD218a, CD183, CD3 epsilon and CD9), not previously reported. (
  • Our study not only confirmed cell surface antigens previously reported to be related to HIV disease stages, but also identified 5 novel ones. (
  • For the first time our study shows how density of cell surface antigens could be efficiently exploited in an array manner in relation to HIV disease stages. (
  • The recently developed antibody microarray technology enables the simultaneous analysis of a large number of cell surface antigens on a single chip. (
  • 12 ], who used a similar antibody microarray to demonstrate the conservation of unique cell surface antigen mosaics in cryopreserved PBMCs from HIV+ individuals. (
  • Antigens, CD29" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (
  • Flow cytometry analysis (surface staining) of human peripheral blood with anti-human CD29 (MEM-101A) biotin, streptavidin-APC. (
  • To date, the immunophenotyping of CD antigens relies on flow cytometry. (
  • CD29 is expressed on resting and activated leukocytes and is a marker for all of the very late activation antigens on cells. (
  • This first-in-class antibody against the CD29 molecule blocks Ebola virus entry into human cells in laboratory models. (
  • MA5-11429 has been used successfully in the western blot analysis of integrin beta 1 (CD29) using human cells lysates. (
  • Calcipotriol inhibits the proliferation of hyperproliferative CD29 positive keratinocytes in psoriatic epidermis in the absence of an effect on the function and number of antigen-presenting cells. (
  • In this environment, T cells are suppressed irrespective of the nature of the antigens these cells could encounter. (
  • The most effective means to secure such contact is the antigen-specific interaction between MDSC and T cells. (
  • Fig. 1 GC B cells engage antigen through BCRs concentrated in F-actin and ezrin-rich pod-like structures. (
  • A ) DIC, IRM, and merged images of immune synapses of live naïve B cells placed on antigen-containing PLB and live LZ GC B cells placed on antigen-containing PLB or PLB with no antigen. (
  • C ) Membrane movement in the immune synapses of live naïve, LZ GC, and DZ GC B cells with time on antigen-containing PLB imaged by IRM. (
  • D ) STED super-resolution images of F-actin formed in immune synapses of naïve B cells and GC B cells placed on PLB that contained antigen. (
  • E ) Colocalization of F-actin and BCR in immune synapses of naïve and LZ GC B cells imaged by confocal microscopy on PLB that contained antigen. (
  • G ) Bottom and orthogonal views of F-actin (green) and BCR (red) in naïve and LZ GC B cells imaged by confocal microscopy on antigen-containing PLB. (
  • The level of plasma fibronectin, a ligand of CD29, correlated with the number of interleukin-10 spot-forming cells. (
  • The MS cells also express MHC class I antigens but do not express class II antigens, although they are inducible by treatment with interferon gamma (IFN-γ). (
  • Sixty-eight percent of the implant-associated T cells coexpressed CD4 and CD29, while only 3% of the T cells coexpressed CD4 and CD45RO. (
  • The expression of HLA-DR and the predominance of CD29+ CD4+ T cells indicate that there is immune activation with the potential for stimulating antigen-specific antibody production. (
  • The CD29 antigen is the 130 kDa integrin β1 chain which is expressed as a heterodimer, non-covalently associated with the integrin α subunits 1 to 6 (CD49a to CD49f) also known as Very Late Antigens (VLA). (
  • CD29 forms non-covalent bonds with the integrin alpha subunits, including CD51 and CD49a-f, to form heterodimers. (
  • 8, 2014 /PRNewswire-iReach/ -- OncoSynergy announced that it has raised over 50% of their crowdfunding goal to support a study testing their experimental drug targeting the CD29 molecule, OS2966, for ebola infection. (
  • Today, the HLDA Workshop meeting has been held 10 times and has over 371 CD antigens molecule have been identified. (
  • In association with CD49a-f, CD29 forms the VLA-1 through VLA-6 complexes, respectively. (
  • FACS analysis of human peripheral blood monocytes using GTX75707 Integrin beta 1 / CD29 antibody [12G10]. (