Substances that are recognized by the immune system and induce an immune reaction.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
Differentiation antigens found on thymocytes and on cytotoxic and suppressor T-lymphocytes. CD8 antigens are members of the immunoglobulin supergene family and are associative recognition elements in MHC (Major Histocompatibility Complex) Class I-restricted interactions.
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
Substances elaborated by bacteria that have antigenic activity.
A bifunctional enzyme that catalyzes the synthesis and HYDROLYSIS of CYCLIC ADP-RIBOSE (cADPR) from NAD+ to ADP-RIBOSE. It is a cell surface molecule which is predominantly expressed on LYMPHOID CELLS and MYELOID CELLS.
Glycoproteins found on immature hematopoietic cells and endothelial cells. They are the only molecules to date whose expression within the blood system is restricted to a small number of progenitor cells in the bone marrow.
Differentiation antigens expressed on B-lymphocytes and B-cell precursors. They are involved in regulation of B-cell proliferation.
A member of the tumor necrosis factor receptor superfamily with specificity for CD40 LIGAND. It is found on mature B-LYMPHOCYTES and some EPITHELIAL CELLS, lymphoid DENDRITIC CELLS. Evidence suggests that CD40-dependent activation of B-cells is important for generation of memory B-cells within the germinal centers. Mutations of the gene for CD40 antigen result in HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 3. Signaling of the receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
A membrane glycoprotein and differentiation antigen expressed on the surface of T-cells that binds to CD40 ANTIGENS on B-LYMPHOCYTES and induces their proliferation. Mutation of the gene for CD40 ligand is a cause of HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 1.
Unglycosylated phosphoproteins expressed only on B-cells. They are regulators of transmembrane Ca2+ conductance and thought to play a role in B-cell activation and proliferation.
Substances elaborated by viruses that have antigenic activity.
Costimulatory T-LYMPHOCYTE receptors that have specificity for CD80 ANTIGEN and CD86 ANTIGEN. Activation of this receptor results in increased T-cell proliferation, cytokine production and promotion of T-cell survival.
Acidic sulfated integral membrane glycoproteins expressed in several alternatively spliced and variable glycosylated forms on a wide variety of cell types including mature T-cells, B-cells, medullary thymocytes, granulocytes, macrophages, erythrocytes, and fibroblasts. CD44 antigens are the principle cell surface receptors for hyaluronate and this interaction mediates binding of lymphocytes to high endothelial venules. (From Abbas et al., Cellular and Molecular Immunology, 2d ed, p156)
Differentiation antigens expressed on pluripotential hematopoietic cells, most human thymocytes, and a major subset of peripheral blood T-lymphocytes. They have been implicated in integrin-mediated cellular adhesion and as signalling receptors on T-cells.
Glycolipid-anchored membrane glycoproteins expressed on cells of the myelomonocyte lineage including monocytes, macrophages, and some granulocytes. They function as receptors for the complex of lipopolysaccharide (LPS) and LPS-binding protein.
Glycoprotein members of the immunoglobulin superfamily which participate in T-cell adhesion and activation. They are expressed on most peripheral T-lymphocytes, natural killer cells, and thymocytes, and function as co-receptors or accessory molecules in the T-cell receptor complex.
Ratio of T-LYMPHOCYTES that express the CD4 ANTIGEN to those that express the CD8 ANTIGEN. This value is commonly assessed in the diagnosis and staging of diseases affecting the IMMUNE SYSTEM including HIV INFECTIONS.
Glycoproteins expressed on all mature T-cells, thymocytes, and a subset of mature B-cells. Antibodies specific for CD5 can enhance T-cell receptor-mediated T-cell activation. The B-cell-specific molecule CD72 is a natural ligand for CD5. (From Abbas et al., Cellular and Molecular Immunology, 2d ed, p156)
Antigens expressed primarily on the membranes of living cells during sequential stages of maturation and differentiation. As immunologic markers they have high organ and tissue specificity and are useful as probes in studies of normal cell development as well as neoplastic transformation.
A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.
Glycoproteins expressed on cortical thymocytes and on some dendritic cells and B-cells. Their structure is similar to that of MHC Class I and their function has been postulated as similar also. CD1 antigens are highly specific markers for human LANGERHANS CELLS.
Antibodies produced by a single clone of cells.
The 140 kDa isoform of NCAM (neural cell adhesion molecule) containing a transmembrane domain and short cytoplasmic tail. It is expressed by all lymphocytes mediating non-MHC restricted cytotoxicity and is present on some neural tissues and tumors.
Antigens expressed on the cell membrane of T-lymphocytes during differentiation, activation, and normal and neoplastic transformation. Their phenotypic characterization is important in differential diagnosis and studies of thymic ontogeny and T-cell function.
A membrane-bound or cytosolic enzyme that catalyzes the synthesis of CYCLIC ADP-RIBOSE (cADPR) from nicotinamide adenine dinucleotide (NAD). This enzyme generally catalyzes the hydrolysis of cADPR to ADP-RIBOSE, as well, and sometimes the synthesis of cyclic ADP-ribose 2' phosphate (2'-P-cADPR) from NADP.
Surface antigens expressed on myeloid cells of the granulocyte-monocyte-histiocyte series during differentiation. Analysis of their reactivity in normal and malignant myelomonocytic cells is useful in identifying and classifying human leukemias and lymphomas.
A costimulatory ligand expressed by ANTIGEN-PRESENTING CELLS that binds to CTLA-4 ANTIGEN with high specificity and to CD28 ANTIGEN with low specificity. The interaction of CD80 with CD28 ANTIGEN provides a costimulatory signal to T-LYMPHOCYTES, while its interaction with CTLA-4 ANTIGEN may play a role in inducing PERIPHERAL TOLERANCE.
Tetraspanin proteins found at high levels in cells of the lymphoid-myeloid lineage. CD53 antigens may be involved regulating the differentiation of T-LYMPHOCYTES and the activation of B-LYMPHOCYTES.
A cell adhesion protein that was originally identified as a heat stable antigen in mice. It is involved in METASTASIS and is highly expressed in many NEOPLASMS.
Zinc-binding metalloproteases that are members of the type II integral membrane metalloproteases. They are expressed by GRANULOCYTES; MONOCYTES; and their precursors as well as by various non-hematopoietic cells. They release an N-terminal amino acid from a peptide, amide or arylamide.
Any part or derivative of any protozoan that elicits immunity; malaria (Plasmodium) and trypanosome antigens are presently the most frequently encountered.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
A costimulatory ligand expressed by ANTIGEN-PRESENTING CELLS that binds to CD28 ANTIGEN with high specificity and to CTLA-4 ANTIGEN with low specificity. The interaction of CD86 with CD28 ANTIGEN provides a stimulatory signal to T-LYMPHOCYTES, while its interaction with CTLA-4 ANTIGEN may play a role in inducing PERIPHERAL TOLERANCE.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.
A tumor necrosis factor receptor subtype found in a variety of tissues and on activated LYMPHOCYTES. It has specificity for FAS LIGAND and plays a role in regulation of peripheral immune responses and APOPTOSIS. Multiple isoforms of the protein exist due to multiple ALTERNATIVE SPLICING. The activated receptor signals via a conserved death domain that associates with specific TNF RECEPTOR-ASSOCIATED FACTORS in the CYTOPLASM.
Antigens determined by leukocyte loci found on chromosome 6, the major histocompatibility loci in humans. They are polypeptides or glycoproteins found on most nucleated cells and platelets, determine tissue types for transplantation, and are associated with certain diseases.
Membrane antigens associated with maturation stages of B-lymphocytes, often expressed in tumors of B-cell origin.
High-molecular weight glycoproteins uniquely expressed on the surface of LEUKOCYTES and their hemopoietic progenitors. They contain a cytoplasmic protein tyrosine phosphatase activity which plays a role in intracellular signaling from the CELL SURFACE RECEPTORS. The CD45 antigens occur as multiple isoforms that result from alternative mRNA splicing and differential usage of three exons.
Process of classifying cells of the immune system based on structural and functional differences. The process is commonly used to analyze and sort T-lymphocytes into subsets based on CD antigens by the technique of flow cytometry.
Substances of fungal origin that have antigenic activity.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The major group of transplantation antigens in the mouse.
A 67-kDa sialic acid binding lectin that is specific for MYELOID CELLS and MONOCYTE-MACROPHAGE PRECURSOR CELLS. This protein is the smallest siglec subtype and contains a single immunoglobulin C2-set domain. It may play a role in intracellular signaling via its interaction with SHP-1 PROTEIN-TYROSINE PHOSPHATASE and SHP-2 PROTEIN-TYROSINE PHOSPHATASE.
Any part or derivative of a helminth that elicits an immune reaction. The most commonly seen helminth antigens are those of the schistosomes.
Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.
Cell-surface glycoprotein beta-chains that are non-covalently linked to specific alpha-chains of the CD11 family of leukocyte-adhesion molecules (RECEPTORS, LEUKOCYTE-ADHESION). A defect in the gene encoding CD18 causes LEUKOCYTE-ADHESION DEFICIENCY SYNDROME.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
A member of the tumor necrosis factor receptor superfamily that may play a role in the regulation of NF-KAPPA B and APOPTOSIS. They are found on activated T-LYMPHOCYTES; B-LYMPHOCYTES; NEUTROPHILS; EOSINOPHILS; MAST CELLS and NK CELLS. Overexpression of CD30 antigen in hematopoietic malignancies make the antigen clinically useful as a biological tumor marker. Signaling of the receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
Glycoproteins found on the membrane or surface of cells.
A critical subpopulation of regulatory T-lymphocytes involved in MHC Class I-restricted interactions. They include both cytotoxic T-lymphocytes (T-LYMPHOCYTES, CYTOTOXIC) and CD8+ suppressor T-lymphocytes.
Sites on an antigen that interact with specific antibodies.
A subtype of tetraspanin proteins that play a role in cell adhesion, cell motility, and tumor metastasis. CD9 antigens take part in the process of platelet activation and aggregation, the formation of paranodal junctions in neuronal tissue, and the fusion of sperm with egg.
A glycoprotein that is secreted into the luminal surface of the epithelia in the gastrointestinal tract. It is found in the feces and pancreaticobiliary secretions and is used to monitor the response to colon cancer treatment.
A subclass of HLA-D antigens that consist of alpha and beta chains. The inheritance of HLA-DR antigens differs from that of the HLA-DQ ANTIGENS and HLA-DP ANTIGENS.
A trisaccharide antigen expressed on glycolipids and many cell-surface glycoproteins. In the blood the antigen is found on the surface of NEUTROPHILS; EOSINOPHILS; and MONOCYTES. In addition, CD15 antigen is a stage-specific embryonic antigen.
Those proteins recognized by antibodies from serum of animals bearing tumors induced by viruses; these proteins are presumably coded for by the nucleic acids of the same viruses that caused the neoplastic transformation.
Established cell cultures that have the potential to propagate indefinitely.
A sialic acid-rich protein and an integral cell membrane mucin. It plays an important role in activation of T-LYMPHOCYTES.
Leukocyte differentiation antigens and major platelet membrane glycoproteins present on MONOCYTES; ENDOTHELIAL CELLS; PLATELETS; and mammary EPITHELIAL CELLS. They play major roles in CELL ADHESION; SIGNAL TRANSDUCTION; and regulation of angiogenesis. CD36 is a receptor for THROMBOSPONDINS and can act as a scavenger receptor that recognizes and transports oxidized LIPOPROTEINS and FATTY ACIDS.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A group of three different alpha chains (CD11a, CD11b, CD11c) that are associated with an invariant CD18 beta chain (ANTIGENS, CD18). The three resulting leukocyte-adhesion molecules (RECEPTORS, LEUKOCYTE ADHESION) are LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN-1; MACROPHAGE-1 ANTIGEN; and ANTIGEN, P150,95.
Large, transmembrane, non-covalently linked glycoproteins (alpha and beta). Both chains can be polymorphic although there is more structural variation in the beta chains. The class II antigens in humans are called HLA-D ANTIGENS and are coded by a gene on chromosome 6. In mice, two genes named IA and IE on chromosome 17 code for the H-2 antigens. The antigens are found on B-lymphocytes, macrophages, epidermal cells, and sperm and are thought to mediate the competence of and cellular cooperation in the immune response. The term IA antigens used to refer only to the proteins encoded by the IA genes in the mouse, but is now used as a generic term for any class II histocompatibility antigen.
A group of antigens that includes both the major and minor histocompatibility antigens. The former are genetically determined by the major histocompatibility complex. They determine tissue type for transplantation and cause allograft rejections. The latter are systems of allelic alloantigens that can cause weak transplant rejection.
Small glycoproteins found on both hematopoietic and non-hematopoietic cells. CD59 restricts the cytolytic activity of homologous complement by binding to C8 and C9 and blocking the assembly of the membrane attack complex. (From Barclay et al., The Leukocyte Antigen FactsBook, 1993, p234)
IMMUNOGLOBULINS on the surface of B-LYMPHOCYTES. Their MESSENGER RNA contains an EXON with a membrane spanning sequence, producing immunoglobulins in the form of type I transmembrane proteins as opposed to secreted immunoglobulins (ANTIBODIES) which do not contain the membrane spanning segment.
Nuclear antigen with a role in DNA synthesis, DNA repair, and cell cycle progression. PCNA is required for the coordinated synthesis of both leading and lagging strands at the replication fork during DNA replication. PCNA expression correlates with the proliferation activity of several malignant and non-malignant cell types.
Oligosaccharide antigenic determinants found principally on NK cells and T-cells. Their role in the immune response is poorly understood.
A transmembrane protein belonging to the tumor necrosis factor superfamily that specifically binds to CD27 ANTIGEN. It is found on activated T-LYMPHOCYTES; B-LYMPHOCYTES; and DENDRITIC CELLS where it plays a role in stimulating the proliferation of CD4-POSITIVE T-LYMPHOCYTES and CD8-POSITIVE T-LYMPHOCYTES.
A ubiquitously expressed complement receptor that binds COMPLEMENT C3B and COMPLEMENT C4B and serves as a cofactor for their inactivation. CD46 also interacts with a wide variety of pathogens and mediates immune response.
A class of animal lectins that bind to carbohydrate in a calcium-dependent manner. They share a common carbohydrate-binding domain that is structurally distinct from other classes of lectins.
Glycoproteins with a wide distribution on hematopoietic and non-hematopoietic cells and strongly expressed on macrophages. CD58 mediates cell adhesion by binding to CD2; (ANTIGENS, CD2); and this enhances antigen-specific T-cell activation.
55-kDa antigens found on HELPER-INDUCER T-LYMPHOCYTES and on a variety of other immune cell types. CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in MAJOR HISTOCOMPATIBILITY COMPLEX class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as INTERLEUKIN-15 receptors and bind to the HIV receptors, binding directly to the HIV ENVELOPE PROTEIN GP120.
A ubiquitously expressed membrane glycoprotein. It interacts with a variety of INTEGRINS and mediates responses to EXTRACELLULAR MATRIX PROTEINS.
A CD antigen that contains a conserved I domain which is involved in ligand binding. When combined with CD18 the two subunits form MACROPHAGE-1 ANTIGEN.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A glycoprotein that is a kallikrein-like serine proteinase and an esterase, produced by epithelial cells of both normal and malignant prostate tissue. It is an important marker for the diagnosis of prostate cancer.
An integrin alpha subunit of approximately 150-kDa molecular weight. It is expressed at high levels on monocytes and combines with CD18 ANTIGEN to form the cell surface receptor INTEGRIN ALPHAXBETA2. The subunit contains a conserved I-domain which is characteristic of several of alpha integrins.
The lipopolysaccharide-protein somatic antigens, usually from gram-negative bacteria, important in the serological classification of enteric bacilli. The O-specific chains determine the specificity of the O antigens of a given serotype. O antigens are the immunodominant part of the lipopolysaccharide molecule in the intact bacterial cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
A specific HLA-A surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-A*02 allele family.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Progenitor cells from which all blood cells derive.
The number of CD4-POSITIVE T-LYMPHOCYTES per unit volume of BLOOD. Determination requires the use of a fluorescence-activated flow cytometer.
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
Carbohydrate antigens expressed by malignant tissue. They are useful as tumor markers and are measured in the serum by means of a radioimmunoassay employing monoclonal antibodies.
GPI-linked membrane proteins broadly distributed among hematopoietic and non-hematopoietic cells. CD55 prevents the assembly of C3 CONVERTASE or accelerates the disassembly of preformed convertase, thus blocking the formation of the membrane attack complex.
Cell adhesion molecules present on virtually all monocytes, platelets, and granulocytes. CD31 is highly expressed on endothelial cells and concentrated at the junctions between them.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Membrane glycoproteins consisting of an alpha subunit and a BETA 2-MICROGLOBULIN beta subunit. In humans, highly polymorphic genes on CHROMOSOME 6 encode the alpha subunits of class I antigens and play an important role in determining the serological specificity of the surface antigen. Class I antigens are found on most nucleated cells and are generally detected by their reactivity with alloantisera. These antigens are recognized during GRAFT REJECTION and restrict cell-mediated lysis of virus-infected cells.
Tetraspanin proteins that are involved in a variety of cellular functions including BASEMENT MEMBRANE assembly, and in the formation of a molecular complexes on the surface of LYMPHOCYTES.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
A member of the tumor necrosis factor receptor superfamily that is specific for 4-1BB LIGAND. It is found in a variety of immune cell types including activated T-LYMPHOCYTES; NATURAL KILLER CELLS; and DENDRITIC CELLS. Activation of the receptor on T-LYMPHOCYTES plays a role in their expansion, production of cytokines and survival. Signaling by the activated receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Proteins prepared by recombinant DNA technology.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.
Polymorphic class I human histocompatibility (HLA) surface antigens present on almost all nucleated cells. At least 20 antigens have been identified which are encoded by the A locus of multiple alleles on chromosome 6. They serve as targets for T-cell cytolytic responses and are involved with acceptance or rejection of tissue/organ grafts.
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).
Receptors present on activated T-LYMPHOCYTES and B-LYMPHOCYTES that are specific for INTERLEUKIN-2 and play an important role in LYMPHOCYTE ACTIVATION. They are heterotrimeric proteins consisting of the INTERLEUKIN-2 RECEPTOR ALPHA SUBUNIT, the INTERLEUKIN-2 RECEPTOR BETA SUBUNIT, and the INTERLEUKIN RECEPTOR COMMON GAMMA-CHAIN.
Sets of cell surface antigens located on BLOOD CELLS. They are usually membrane GLYCOPROTEINS or GLYCOLIPIDS that are antigenically distinguished by their carbohydrate moieties.
Those hepatitis B antigens found on the surface of the Dane particle and on the 20 nm spherical and tubular particles. Several subspecificities of the surface antigen are known. These were formerly called the Australia antigen.
Ubiquitously-expressed tetraspanin proteins that are found in late ENDOSOMES and LYSOSOMES and have been implicated in intracellular transport of proteins.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Tetraspanin proteins found associated with LAMININ-binding INTEGRINS. The CD151 antigens may play a role in the regulation of CELL MOTILITY.
A component of the B-cell antigen receptor that is involved in B-cell antigen receptor heavy chain transport to the PLASMA MEMBRANE. It is expressed almost exclusively in B-LYMPHOCYTES and serves as a useful marker for B-cell NEOPLASMS.
An encapsulated lymphatic organ through which venous blood filters.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Human immune-response or Class II antigens found mainly, but not exclusively, on B-lymphocytes and produced from genes of the HLA-D locus. They are extremely polymorphic families of glycopeptides, each consisting of two chains, alpha and beta. This group of antigens includes the -DR, -DQ and -DP designations, of which HLA-DR is most studied; some of these glycoproteins are associated with certain diseases, possibly of immune etiology.
A membrane-bound tumor necrosis family member found primarily on activated T-LYMPHOCYTES that binds specifically to CD30 ANTIGEN. It may play a role in INFLAMMATION and immune regulation.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
A class of enzymes involved in the hydrolysis of the N-glycosidic bond of nitrogen-linked sugars.
A form of undifferentiated malignant LYMPHOMA usually found in central Africa, but also reported in other parts of the world. It is commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. B-cell antigens are expressed on the immature cells that make up the tumor in virtually all cases of Burkitt lymphoma. The Epstein-Barr virus (HERPESVIRUS 4, HUMAN) has been isolated from Burkitt lymphoma cases in Africa and it is implicated as the causative agent in these cases; however, most non-African cases are EBV-negative.
Molecules on the surface of B- and T-lymphocytes that recognize and combine with specific antigens.
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.
An alpha-integrin subunit found on lymphocytes, granulocytes, macrophages and monocytes. It combines with the integrin beta2 subunit (CD18 ANTIGEN) to form LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN-1.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Antigens of the virion of the HEPATITIS B VIRUS or the Dane particle, its surface (HEPATITIS B SURFACE ANTIGENS), core (HEPATITIS B CORE ANTIGENS), and other associated antigens, including the HEPATITIS B E ANTIGENS.
The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.
The processes triggered by interactions of ANTIBODIES with their ANTIGENS.
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
Mice homozygous for the mutant autosomal recessive gene "scid" which is located on the centromeric end of chromosome 16. These mice lack mature, functional lymphocytes and are thus highly susceptible to lethal opportunistic infections if not chronically treated with antibiotics. The lack of B- and T-cell immunity resembles severe combined immunodeficiency (SCID) syndrome in human infants. SCID mice are useful as animal models since they are receptive to implantation of a human immune system producing SCID-human (SCID-hu) hematochimeric mice.
Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
A heterogeneous group of immunocompetent cells that mediate the cellular immune response by processing and presenting antigens to the T-cells. Traditional antigen-presenting cells include MACROPHAGES; DENDRITIC CELLS; LANGERHANS CELLS; and B-LYMPHOCYTES. FOLLICULAR DENDRITIC CELLS are not traditional antigen-presenting cells, but because they hold antigen on their cell surface in the form of IMMUNE COMPLEXES for B-cell recognition they are considered so by some authors.
The type species of LYMPHOCRYPTOVIRUS, subfamily GAMMAHERPESVIRINAE, infecting B-cells in humans. It is thought to be the causative agent of INFECTIOUS MONONUCLEOSIS and is strongly associated with oral hairy leukoplakia (LEUKOPLAKIA, HAIRY;), BURKITT LYMPHOMA; and other malignancies.
T-cell receptors composed of CD3-associated alpha and beta polypeptide chains and expressed primarily in CD4+ or CD8+ T-cells. Unlike immunoglobulins, the alpha-beta T-cell receptors recognize antigens only when presented in association with major histocompatibility (MHC) molecules.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Class I human histocompatibility (HLA) surface antigens encoded by more than 30 detectable alleles on locus B of the HLA complex, the most polymorphic of all the HLA specificities. Several of these antigens (e.g., HLA-B27, -B7, -B8) are strongly associated with predisposition to rheumatoid and other autoimmune disorders. Like other class I HLA determinants, they are involved in the cellular immune reactivity of cytolytic T lymphocytes.
The altered state of immunologic responsiveness resulting from initial contact with antigen, which enables the individual to produce antibodies more rapidly and in greater quantity in response to secondary antigenic stimulus.
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
The phenomenon of target cell destruction by immunologically active effector cells. It may be brought about directly by sensitized T-lymphocytes or by lymphoid or myeloid "killer" cells, or it may be mediated by cytotoxic antibody, cytotoxic factor released by lymphoid cells, or complement.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
A melanosome-specific protein that plays a role in the expression, stability, trafficking, and processing of GP100 MELANOMA ANTIGEN, which is critical to the formation of Stage II MELANOSOMES. The protein is used as an antigen marker for MELANOMA cells.
A widely distributed cell surface transmembrane glycoprotein that stimulates the synthesis of MATRIX METALLOPROTEINASES. It is found at high levels on the surface of malignant NEOPLASMS and may play a role as a mediator of malignant cell behavior.
A general term for various neoplastic diseases of the lymphoid tissue.
An albumin obtained from the white of eggs. It is a member of the serpin superfamily.
Antigens associated with specific proteins of the human adult T-cell immunodeficiency virus (HIV); also called HTLV-III-associated and lymphadenopathy-associated virus (LAV) antigens.
An inhibitory T CELL receptor that is closely related to CD28 ANTIGEN. It has specificity for CD80 ANTIGEN and CD86 ANTIGEN and acts as a negative regulator of peripheral T cell function. CTLA-4 antigen is believed to play role in inducing PERIPHERAL TOLERANCE.
A promyelocytic cell line derived from a patient with ACUTE PROMYELOCYTIC LEUKEMIA. HL-60 cells lack specific markers for LYMPHOID CELLS but express surface receptors for FC FRAGMENTS and COMPLEMENT SYSTEM PROTEINS. They also exhibit phagocytic activity and responsiveness to chemotactic stimuli. (From Hay et al., American Type Culture Collection, 7th ed, pp127-8)
A widely expressed transmembrane glycoprotein that functions as a METASTASIS suppressor protein. It is underexpressed in a variety of human NEOPLASMS.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
A group of differentiation surface antigens, among the first to be discovered on thymocytes and T-lymphocytes. Originally identified in the mouse, they are also found in other species including humans, and are expressed on brain neurons and other cells.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
The specific failure of a normally responsive individual to make an immune response to a known antigen. It results from previous contact with the antigen by an immunologically immature individual (fetus or neonate) or by an adult exposed to extreme high-dose or low-dose antigen, or by exposure to radiation, antimetabolites, antilymphocytic serum, etc.
Manifestations of the immune response which are mediated by antigen-sensitized T-lymphocytes via lymphokines or direct cytotoxicity. This takes place in the absence of circulating antibody or where antibody plays a subordinate role.
A single, unpaired primary lymphoid organ situated in the MEDIASTINUM, extending superiorly into the neck to the lower edge of the THYROID GLAND and inferiorly to the fourth costal cartilage. It is necessary for normal development of immunologic function early in life. By puberty, it begins to involute and much of the tissue is replaced by fat.
Endogenous tissue constituents that have the ability to interact with AUTOANTIBODIES and cause an immune response.
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
Nuclear antigens encoded by VIRAL GENES found in HUMAN HERPESVIRUS 4. At least six nuclear antigens have been identified.
A soluble substance elaborated by antigen- or mitogen-stimulated T-LYMPHOCYTES which induces DNA synthesis in naive lymphocytes.
A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.
A cell line derived from cultured tumor cells.
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
A sex-specific cell surface antigen produced by the sex-determining gene of the Y chromosome in mammals. It causes syngeneic grafts from males to females to be rejected and interacts with somatic elements of the embryologic undifferentiated gonad to produce testicular organogenesis.
A cell adhesion molecule of the immunoglobulin superfamily that is expressed in ENDOTHELIAL CELLS and is involved in INTERCELLULAR JUNCTIONS.
Antigens stimulating the formation of, or combining with heterophile antibodies. They are cross-reacting antigens found in phylogenetically unrelated species.
CD4-positive T cells that inhibit immunopathology or autoimmune disease in vivo. They inhibit the immune response by influencing the activity of other cell types. Regulatory T-cells include naturally occurring CD4+CD25+ cells, IL-10 secreting Tr1 cells, and Th3 cells.
Antibodies obtained from a single clone of cells grown in mice or rats.
Antigenic determinants recognized and bound by the T-cell receptor. Epitopes recognized by the T-cell receptor are often located in the inner, unexposed side of the antigen, and become accessible to the T-cell receptors after proteolytic processing of the antigen.
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
A heterodimeric protein that is a cell surface antigen associated with lymphocyte activation. The initial characterization of this protein revealed one identifiable heavy chain (ANTIGENS, CD98 HEAVY CHAIN) and an indeterminate smaller light chain. It is now known that a variety of light chain subunits (ANTIGENS, CD98 LIGHT CHAINS) can dimerize with the heavy chain. Depending upon its light chain composition a diverse array of functions can be found for this protein. Functions include: type L amino acid transport, type y+L amino acid transport and regulation of cellular fusion.
The hepatitis B antigen within the core of the Dane particle, the infectious hepatitis virion.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
The complex formed by the binding of antigen and antibody molecules. The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES.
They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system.
The sum of the weight of all the atoms in a molecule.
Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.
A group of the D-related HLA antigens found to differ from the DR antigens in genetic locus and therefore inheritance. These antigens are polymorphic glycoproteins comprising alpha and beta chains and are found on lymphoid and other cells, often associated with certain diseases.
Immunoglobulins produced in response to VIRAL ANTIGENS.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
A glycolipid, cross-species antigen that induces production of antisheep hemolysin. It is present on the tissue cells of many species but absent in humans. It is found in many infectious agents.
Elements of limited time intervals, contributing to particular results or situations.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
An inhibitory B7 antigen that has specificity for the T-CELL receptor PROGRAMMED CELL DEATH 1 PROTEIN. CD274 antigen provides negative signals that control and inhibit T-cell responses and is found at higher than normal levels on tumor cells, suggesting its potential role in TUMOR IMMUNE EVASION.
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
A species of POLYOMAVIRUS originally isolated from Rhesus monkey kidney tissue. It produces malignancy in human and newborn hamster kidney cell cultures.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
Substances that augment, stimulate, activate, potentiate, or modulate the immune response at either the cellular or humoral level. The classical agents (Freund's adjuvant, BCG, Corynebacterium parvum, et al.) contain bacterial antigens. Some are endogenous (e.g., histamine, interferon, transfer factor, tuftsin, interleukin-1). Their mode of action is either non-specific, resulting in increased immune responsiveness to a wide variety of antigens, or antigen-specific, i.e., affecting a restricted type of immune response to a narrow group of antigens. The therapeutic efficacy of many biological response modifiers is related to their antigen-specific immunoadjuvanticity.
Antigens that exist in alternative (allelic) forms in a single species. When an isoantigen is encountered by species members who lack it, an immune response is induced. Typical isoantigens are the BLOOD GROUP ANTIGENS.
Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell.
A melanosome-associated protein that plays a role in the maturation of the MELANOSOME.
The genetic region which contains the loci of genes which determine the structure of the serologically defined (SD) and lymphocyte-defined (LD) TRANSPLANTATION ANTIGENS, genes which control the structure of the IMMUNE RESPONSE-ASSOCIATED ANTIGENS, HUMAN; the IMMUNE RESPONSE GENES which control the ability of an animal to respond immunologically to antigenic stimuli, and genes which determine the structure and/or level of the first four components of complement.
Bone marrow-derived lymphocytes that possess cytotoxic properties, classically directed against transformed and virus-infected cells. Unlike T CELLS; and B CELLS; NK CELLS are not antigen specific. The cytotoxicity of natural killer cells is determined by the collective signaling of an array of inhibitory and stimulatory CELL SURFACE RECEPTORS. A subset of T-LYMPHOCYTES referred to as NATURAL KILLER T CELLS shares some of the properties of this cell type.
A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
Form of passive immunization where previously sensitized immunologic agents (cells or serum) are transferred to non-immune recipients. When transfer of cells is used as a therapy for the treatment of neoplasms, it is called adoptive immunotherapy (IMMUNOTHERAPY, ADOPTIVE).

Immunotherapy of human tumors with T-cell-activating bispecific antibodies: stimulation of cytotoxic pathways in vivo. (1/629)

Bispecific monoclonal antibodies (Bi-mAbs) specific for a tumor-associated antigen and the CD3 or CD28 antigen on T lymphocytes represent one of the most successful experimental strategies for the immunotherapy of cancer. We report that the in vivo administration of both alpha-CD3/CD30 and alpha-CD28/CD30 Bi-mAbs results in the specific activation of xenotransplanted, resting human T cells infiltrating the CD30-positive Hodgkin's tumor. Bi-mAb treatment resulted in enhanced expression of cytokines such as interleukin 1beta, interleukin 2, tumor necrosis factor type alpha, and activation markers including Ki-67, CD25, and CD45RO in tumor-infiltrating lymphocytes. This antigen-dependent, local T-cell stimulation led to the activation of the cytolytic machinery in T lymphocytes, determined by the up-regulation of mRNA-encoding perforin and the cytotoxic serine-esterases granzymes A and B. The Bi-mAb-induced generation of CTLs depended on the presence of the CD30 antigen and the combined application of both Bi-mAbs. Our findings suggest that the combined application of T-cell-activating Bi-mAbs is able to achieve a tumor site-specific activation of the T-cell cytolytic machinery in vivo. The fact that these cytotoxic cells do not home in tumor-associated antigen-negative tissue and do not enter circulation might explain our previous observations (C. Renner et al., Blood, 87: 2930-2937, 1996) of a high cure rate in preclinical models even at an advanced stage of disease.  (+info)

The T-cell activation markers CD30 and OX40/CD134 are expressed in nonoverlapping subsets of peripheral T-cell lymphoma. (2/629)

The tumor necrosis factor (TNF) receptor family includes several important markers of activation in T cells. We examined expression patterns of two T-cell-associated members of these receptors, namely CD30 and OX40/CD134, in 148 cases of T-cell lymphoma to identify possible objective immunohistochemical criteria for subclassification of these tumors. CD30 expression was characteristic of tumors with an anaplastic (46/47 cases [98%]) or large-cell (10/21 [48%]) morphology and was seen in only scattered cells in other tumor types. In contrast, large numbers of OX40/CD134(+) tumors cells were typical of angioimmunoblastic lymphoma (15/16 [94%]), angiocentric lymphoma (4/4), a subset of large-cell lymphomas (10/21 [48%]), and lymphomas with a prominent histiocytic component (6/7 [86%]). Strong OX40/CD134 and CD30 coexpression was seen in only 4% of tumors, typically those with an anaplastic/Hodgkin's-like appearance. OX40/CD134 expression was characteristic of tumors composed of activated CD4(+) T cells and was not seen in small-cell T-cell lymphomas, lymphoblastic lymphomas, or other tumor types, including B-cell lymphomas or carcinomas. These results suggest that immunostaining for OX40/CD134 may be helpful in subclassification of peripheral T-cell lymphomas and that the patterns of TNF receptor family expression in these tumors may parallel those seen within nonneoplastic helper T-cell subsets.  (+info)

Cerebrospinal fluid concentrations of soluble CD27 in HTLV-I associated myelopathy and multiple sclerosis. (3/629)

OBJECTIVES: Stimulation of T lymphocytes via the T cell receptor strongly enhances CD27 membrane expression and induces the release of a soluble 32 kDa form of CD27 (sCD27). CD27 is a member of the TNF receptor family, a group of molecules that have important roles in lymphocyte differentiation and survival. Raised concentrations of sCD27 have been reported in various immunopathological conditions and there is evidence that this molecule can serve as a marker of T cell activation in vivo. Concentrations of sCD27 in CSF were compared between patients with T cell mediated neurological disease and non-inflammatory controls. Also, the relation of CSF-sCD27 concentrations with clinical disease activity was investigated in patients with multiple sclerosis. METHODS: Four groups were studied: (1) eight patients with HTLV-1 associated myelopathy/ tropical spastic paraparisis (HAM)/TSP), (2) eight HTLV-I carriers, (3) 41 patients with multiple sclerosis, and (4) 43 patients with other neurological disease (OND). Concentrations of CSF-sCD27 were determined by enzyme linked immunosorbent assay (ELISA). RESULTS: Quantification of CSF-sCD27 differentiates patients with HAM/TSP from HTLV-I carriers (p<0.01) and from patients with OND (p<0.001). Moreover, the multiple sclerosis patient group was different from the OND group (p<0.0001). In patients with multiple sclerosis, CSF-sCD27 concentrations were higher in 24 patients with clinically active disease than in 17 with clinically stable disease. In addition, most of the patients with multiple sclerosis with high sCD27 concentrations showed an increase in EDSS, whereas none of the patients with low sCD27 had an EDSS increase. CONCLUSIONS: As a reliable marker of immunological disease activity in inflammatory white matter disease is still not available, it is proposed that quantification of CSF-sCD27 concentrations is a good candidate. Also, it may serve as a tool to stratify neurological diseases in inflammatory and non-inflammatory states.  (+info)

Early reduction of the over-expression of CD40L, OX40 and Fas on T cells in HIV-1 infection during triple anti-retroviral therapy: possible implications for lymphocyte traffic and functional recovery. (4/629)

Fas, CD40L and OX40 are members of the tumour necrosis factor (TNF) receptor superfamily with critical roles in T cell activation and death, B cell function, dendritic cell maturation and leucocyte traffic regulation. The aim of this study was to evaluate the effects of anti-retroviral therapy (HAART) on CD40L, OX40 and Fas expression on freshly isolated peripheral blood T cells by three-colour flow cytometry and compare them with lymphoproliferative responses, peripheral blood cell counts and viral load. Fourteen asymptomatic HIV-1+ patients treated with Lamivudine, Stavudine and Nelfinavir were prospectively investigated sequentially for 48 weeks. At baseline, patients exhibited significantly enhanced proportions and counts of CD40L+ and OX40+ cells within the CD4 subset which were corrected by weeks 8-16 of HAART. Interestingly, in the five patients showing viral load rebound during therapy in spite of increasing CD4 counts, the reduction of the levels of these costimulatory molecules was similarly maintained. Therapy induced a decrease in the over-expression of Fas, particularly in the CD4 subset where normal levels were reached at week 8. This reduction occurred in parallel with the major recovery of lymphoproliferative responses. Higher basal levels and lower reduction of Fas were associated with suboptimal suppression of viraemia. In conclusion, this previously undescribed increased expression of CD40L and OX40 may play a role in the HIV-associated pan-immune activation and represent a possible target for immunointervention, as suggested for several immunologically mediated diseases. Moreover, HAART induced an early correction of the over-expression of Fas, CD40L and OX40 in CD4 T cells which could be involved in the recovery of the cell traffic disturbances and in the T cell renewal capacity.  (+info)

CD28-independent costimulation of T cells by OX40 ligand and CD70 on activated B cells. (5/629)

OX40 and its ligand (OX40L) have been implicated in T cell-dependent humoral immune responses. To further characterize the role of OX40/OX40L in T-B cell interaction, we newly generated an anti-mouse OX40L mAb (RM134L) that can inhibit the costimulatory activity of OX40L transfectants for anti-CD3-stimulated T cell proliferation. Flow cytometric analyses using RM134L and an anti-mouse OX40 mAb indicated that OX40 was inducible on splenic T cells by stimulation with immobilized anti-CD3 mAb in a CD28-independent manner, while OX40L was not expressed on resting or activated T cells. OX40L was inducible on splenic B cells by stimulation with anti-IgM Ab plus anti-CD40 mAb, but not by either alone. These activated B cells exhibited a potent costimulatory activity for anti-CD3-stimulated T cell proliferation and IL-2 production. Anti-CD80 and anti-CD86 mAbs partially inhibited the costimulatory activity, and further inhibition was obtained by their combination with RM134L and/or anti-CD70 mAb. We also found the anti-IgM Ab- plus anti-CD40 mAb-stimulated B cells exhibited a potent costimulatory activity for proliferation of and IL-2 production by anti-CD3-stimulated CD28- T cells from CD28-deficient mice, which was substantially inhibited by RM134L and/or anti-CD70 mAb. These results indicated that OX40L and CD70 expressed on surface Ig- and CD40-stimulated B cells can provide CD28-independent costimulatory signals to T cells.  (+info)

Expression of T cell activation antigen CD134 (OX40) has no predictive value for the occurrence or response to therapy of acute graft-versus-host disease in partial T cell-depleted bone marrow transplantation. (6/629)

CD134 (OX40) is a member of the tumor necrosis factor family which is expressed by activated T lymphocytes. CD134 expression on T cells was monitored during the first 35 days post-transplant in 14 patients, receiving either an HLA-identical sibling bone marrow transplant (BMT), a matched unrelated transplant (MUD-BMT) or an autologous peripheral blood progenitor cell transplant (PBPCT). The sibling and unrelated grafts were partially depleted of T cells. CD134 expression on CD4+ T cells peaked between 7 and 14 days after BMT, with a mean peak value of 45% of CD4+ cells (range 26-70%) over all three patient groups. The observed pattern of CD4+ CD134+ expression, an increase during the first 2 weeks post-BMT followed by a gradual decline towards values of 15-40%, was similar in all groups. No difference in the kinetics of CD134 expression by CD4+ T cells was observed between the patients that did or did not develop graft-versus-host disease (GVHD), nor did the clinical effect of any treatment given for GVHD correlate with alterations in CD134 expression by CD4+ T cells. Absolute CD4+,CD134+ T cell numbers showed a more rapid increment after autologous PBPCT than after sibling or MUD transplants. We conclude that expression of CD134+ by CD4+ T lymphocytes cannot serve as a surrogate marker for allo-reactivity. CD134+ expression may reflect lymphocyte regeneration, rather than alloreactivity.  (+info)

Evidence that human CD8+CD45RA+CD27- cells are induced by antigen and evolve through extensive rounds of division. (7/629)

We recently showed that circulating human CD8(+) effector cells have a CD45RA+CD27(-) membrane phenotype. In itself this phenotype appeared to pose a paradox: CD45RA, a marker expressed by unprimed cells, combined with absence of CD27, characteristic for chronically stimulated T cells. To investigate whether differentiation towards the CD45RA+CD27(-) phenotype is dependent on antigenic stimulation and involves cellular division, TCR Vbeta usage and telomeric restriction fragment (TRF) length were analyzed within distinct peripheral blood CD8(+) subsets. FACS analysis showed that the TCR Vbeta repertoire of CD8(+)CD45RA+CD27(-) cells differed significantly from that of unprimed CD8(+)CD45RA+CD27(+) cells. Moreover, in two out of six individuals large expansions of particular Vbeta families were observed in the CD8(+)CD45RA+CD27(-) subset. CDR3 spectrotyping and single-strand confirmation analysis revealed that within the CD8(+)CD45RA+CD27(-) population most of the 22 tested Vbeta families were dominated by oligoclonal expansions. The mean TRF length was found to be 2.3+/-1.0 kb shorter in the CD8(+)CD45RA+CD27(-) subset compared with the unprimed CD8(+)CD45RA+CD27(+) population, but did not differ substantially from that of memory type, CD8(+)CD45RA-CD27(+) T cells. These findings indicate that the CD8(+)CD45RA+CD27(-) cytotoxic effector population consists of antigen-induced, clonally expanded cells and confirm that the expression of CD45RA is not a strict marker of antigen non-experienced T cells.  (+info)

Targeted disruption of Traf5 gene causes defects in CD40- and CD27-mediated lymphocyte activation. (8/629)

TRAF5 [tumor necrosis factor (TNF) receptor-associated factor 5] is implicated in NF-kappaB and c-Jun NH(2)-terminal kinase/stress-activated protein kinase activation by members of the TNF receptor superfamily, including CD27, CD30, CD40, and lymphotoxin-beta receptor. To investigate the functional role of TRAF5 in vivo, we generated TRAF5-deficient mice by gene targeting. Activation of either NF-kappaB or c-Jun NH(2)-terminal kinase/stress-activated protein kinase by tumor necrosis factor, CD27, and CD40 was not abrogated in traf5(-/-) mice. However, traf5(-/-) B cells showed defects in proliferation and up-regulation of various surface molecules, including CD23, CD54, CD80, CD86, and Fas in response to CD40 stimulation. Moreover, in vitro Ig production of traf5(-/-) B cells stimulated with anti-CD40 plus IL-4 was reduced substantially. CD27-mediated costimulatory signal also was impaired in traf5(-/-) T cells. Collectively, these results demonstrate that TRAF5 is involved in CD40- and CD27-mediated signaling.  (+info)

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This eighth edition of the United States Code was prepared and published pursuant to section 202 (c) of Title 1 of the Code. It contains a consolidation and codification of all the general and permanent laws of the United States which are in force on January 20, 1971, By statutory authority this edition may be cited U.S.C. 1970 ed. Previous editions were published in 1926, 1934, 1940, 1946, 1952, 1958, and 1964.. Inasmuch as many of the general and permanent laws which are required to be incorporated in this Code are inconsistent, redundant, and obsolete, the Committee on the Judiciary is engaged in a comprehensive project of revising and enacting the Code into law, title by title. In furtherance of this plan bills have been enacted to revise, codify and enact into law Titles 1, 3, 4, 5, 6, 9, 10, 13, 14, 17, 18, 23, 28, 32, 35, 37, 38, 39, and 44. In addition, bills relating to other titles are also being prepared for introduction. When this work is completed all the titles of the Code will ...
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein and is similar in sequence to its family member CD53 antigen. It is known to complex with integrins and other transmembrane 4 superfamily proteins. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008 ...
Exposure of APL as well as non-APL samples to any concentration of As2O3 did not affect the expression of beta2 integrins (CD11a and CD11b), CD45 isoforms (RA, RB and R0), CD44/H-CAM, CD33 and the CEA-related antigen family members CD66ade and CD66b, thus ...
Literatura, Paramaśiva, Śiva e Śakti, entendendo os tattva-s (Paramasiva,Parama siva,Paramashiva,Parama shiva,Paramshiva,Param shiva,Siva,Shiva,Sakti,Shakti) - The sun of Sanskrit knowledge
Original citation: J. Clin. Invest.112:286-297 (2003). doi:10.1172/JCI18025.. Citation for this corrigendum: J. Clin. Invest.113:1069 (2004). doi:10.1172/JCI18025E1.. The legends for Figures 6 and 7 contained inaccuracies, and the correct versions appear below. The conclusions of the article are unaffected.. Figure 6 BAFF increases the generation of ISC from activated memory B cells. (a and b) Memory B cells were preactivated with CD40L and IL-2/IL-10 for 4 days and then recultured with (a) media (black bars), or (b) IL-2/IL-10 alone (black bars) or in the presence of CD40L (white bars) or BAFF (gray bars). Each value represents the mean Ig secretion ± SEM of five (a) or seven (b) experiments using cells from different donors. *P , 0.05; **P , 0.01. (c) Secondary B cell cultures were performed in the absence (black bars) or presence (white bars) of soluble TACI-Ig (20 μg/ml). The values represent the mean IgA ± SD of duplicate samples. (d) Memory B cells were preactivated with ...
M-T271 recognizes CD27, a member of the tumor necrosis factor receptor family. CD27 is expressed at differing levels on memory B cells, a fraction of plasma cells, and on naive and memory T cells, but is not expressed on naive B cells or effector T cells. It is also expressed on NK cells. CD27 therefore represents a useful marker to distinguish certain T and B cell subsets from each other. - Österreich
Activated naive B cells that seed a GC and undergo SHM, Ig isotype switching, and selection by a specific Ag can differentiate into memory B cells or plasma cells. It is generally accepted that the processes of SHM and isotype switching are markers of memory B cells. In human tonsils, memory B cells were historically identified by the loss of IgD together with other markers such as CD38 (5, 10, 11, 17). The case for using IgD and CD38 to separate memory (IgD−CD38−) from naive (IgD+CD38−) and GC (IgD−CD38+) B cells was supported by the finding that the majority of tonsil IgD+ cells expressed unmutated IgV region genes, while those expressed by IgD− cells were mutated (5, 11, 25, 26). Studies using these markers demonstrated that although both naive and memory B cells were in a quiescent state, memory cells exhibited enhanced responses compared to naive B cells in vitro (10, 17, 18, 19, 20). Together, these articles established a scheme to identify human memory B cells.. However, ...
Memory B cells are generated in germinal centers (GC) and contribute to serological immunity by rapidly differentiating into plasma cells. Human memory B cells can be identified by the expression of CD27. These cells exhibit more rapid responses than naive (CD27-) B cells following stimulation in vitro, consistent with the heightened kinetics of secondary responses in vivo. CD27+ B cells express mutated Ig V region genes; however a significant proportion continue to express IgM, suggesting the existence of IgM+ memory B cells. The observation that mutated IgM+CD27+ B cells are generated in humans who cannot form GC led to the conclusions that these cells are generated independently of GC and thus are not memory cells and that they mediate responses to T cell-independent Ag. Although some studies support the idea that IgM+CD27+ B cells participate in T cell-independent responses, many others do not. In this review we will provide alternate interpretations of the biology of IgM+CD27+ B cells and propose
B-cells go through a variety of stages during their development which is discussed in B-cell Development. However, the major functionally important stages to be aware of are the final stages known as the Plasma Cell and Memory B Cell stages. Plasma Cells are B-cells specialized for high levels of antibody synthesis and secretion. Memory B Cells are quiescent antigen-sepecific cells which differentiate following a primary immune response to a particular microbe which can become rapidly activated to differentiate into Plasma Cells if the microbe if re-encountered. B-cells can also be classified based on the subtype of antibody which they secrete ...
Quantitative variation in the expression of MHC-encoded class II (Ia) glycoproteins has been associated with stages of lymphocyte development and a number
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Finally, we compared both the frequency and the numbers of Flu- and CMV-specific T cells before EBV infection with those after resolution of the acute phase of infection. Although acute EBV infection transiently alters the CD8+ T cell compartment, both the frequency and total numbers of CD8+ and CD4+ T cells ,150 d after acute infection are similar to baseline (Fig. 1, D and E), indicating that homeostasis of the peripheral immune compartment is not grossly altered by infectious mononucleosis. Consistent with this, there was no significant loss of either CMV- or Flu- specific memory T cells at later time points after EBV infection (Fig. 5 C). Altogether, these data suggest there is no attrition of peripheral blood memory CD8+ T cells during or after heterologous infections in young adults.. This was surprising because studies in animal models showed attrition of preexisting memory CD8+ T cells in many infections (Selin et al., 1996, 1999; Varga et al., 2001; Smith et al., 2002; Liu et al., 2003; ...
Scientists already knew that the formation of memory B lymphocytes is not as effective in elderly population, putting them at greater risk when facing new pathogens such as the SARS-CoV-2 coronavirus.. The study in Melameds lab revealed that as part of the aging process, existing memory B lymphocytes use hormonal signals to obstruct the production of new ones. As a result, the immune system becomes better at responding to pathogens it encountered before, but less capable of adapting to new threats.. Seeking a solution for this problem, Melameds lab collaborated with the departments of hematology and rheumatology at Tel Aviv Sourasky Medical Center and Rambam Health Care Campus in Haifa, respectively.. They examined elderly patients who had undergone B-cell depletion as a treatment for conditions such as lupus, lymphoma and multiple sclerosis. They found that after a significant amount of memory B lymphocytes were removed, the patients immune system was rejuvenated and began producing new, ...
Free online memory games. metro match is the classic memory game with a twist. Aj and ep are extremes on the spectrum of human memory. and their cases say more than any brain scan about the extent to which our memories make us who we. The game will be opened inside a popup window. please turn off any block popup program before clicking on the play button. Here, we show that human memory b lymphocytes. proliferate and differentiate into plasma cells in response to polyclonal stimuli, .. Is human memory similar to to the ram in a pc? discussion by michael freed, aerospace human factors, nasa ames research center. Exploiting human memory to create secure and emorable passwords they are difficult to remember because there are limits to human memory. Basic perofrmance data were obtained on the effect of critical task variables in unaided multiobject tracking behavior. .forgetmenot. intimate computing in support of human memory. a prototype device for memory support, an example of contextbased ...
Results Twenty patients were included. Nine were treated by etanercept (ETN), 9 by certolizumab pegol and 2 by adalimumab. The percentage of B cells significantly increased under TNFi from (median [IQR 25-75]) 4.6 (3.5-6.7) to 7.6 (5.2-9.9) % of lymphocytes. No change was observed in the different subtypes of B cells. However, in patients treated with ETN, IgD-CD27- double negative memory B cells significantly increased from 4.6 (2.5-5.4) to 7.7 (6.2-11.0)(p=0.03). The variation of those double negative B cells were significantly different from those observed with monoclonal antibodies (+1.6 [0.0-5.4] vs 0.3 [-1.3-1.8]% of B cells, p=0.02). No change of T, NK, NKT cells was observed in either group. EULAR responders at 3 months had significantly higher percentage of CD27+ memory B cells at baseline (32.9 [25.2-40.6] vs 19.5 [12.3-19.6]% of B cells, respectively; p=0.02), especially IgD+CD27+ pre-switch memory B cells (19.3 [9.8-21.8] vs 5.9 [4.9-9.4]% of B cells, respectively; p=0.02). Since ...
The long-term goal of our research is to understand the cellular and molecular basis for B cell memory, an essential component of immunity to most pathogens. We...
Tracer or toxin injections. For tracer experiments, under chloral hydrate anesthesia (7% in saline; 350 mg/kg), a fine glass pipette containing 1.0% cholera toxin subunit B (CTB; List Biologic, Campbell, CA), 12.5% biotinylated dextran (BD), or a mixture of 1% CTB and 12.5% BD was lowered to the precalculated targets based on the rat atlas of Paxinos and Watson (1998), and 9 nl of a solution containing the tracers was injected by an air pressure system. Phaseolus vulgaris leukoagglutinin (PHA-L; 2.5%) was injected by iontophoresis with a current of 5 μA for 15 min (7 s on and 7 s off). After two additional minutes, the pipette was slowly withdrawn and the incision was closed with wound clips. Animals survived for 7 d. The coordinates for tracer injections were as follows: medial prefrontal cortex, anteroposterior (AP), 2.20 mm, medial-lateral (ML), 0.4 mm, dorsoventral (DV), 4.6 mm; midline thalamus, AP, -2.8 mm, ML, 0 mm, DV, 4.4 mm; intralaminar thalamus, AP, -2.8 mm, ML, 0.8 mm, DV, 5.6 mm; ...
Stimulation by antigen through the B cell receptor (BCR) followed by cognate T cell help drives proliferation and differentiation of antigen-specific naı̈ve B lymphocytes into memory B cells and plasma cells (1, 2). Memory B cells carrying somatically mutated immunoglobulin (Ig) genes survive in secondary lymphoid organs in the absence of antigen (3) and mediate secondary immune responses upon rechallenge. In contrast, plasma cells are terminally differentiated, nondividing cells that home to spleen and bone marrow and are the main source of antibody, which they secrete at a high rate. Mouse plasma cells can be long-lived and are able to sustain antibody production for several months in the absence of memory B cells or antigen (4, 5). However, it is less likely that long-lived plasma cells produced during an immune response will maintain a constant supply of specific antibody over a human life-span, because even long-lived plasma cells would eventually need to be replenished over a human ...
The mental faculty that allows us to retain information as well as recall experiences from a long time ago is known as memory. the human memory is a highly. Here, we show that human memory b lymphocytes. proliferate and differentiate into plasma cells in response to polyclonal stimuli, .. It is more accurate to speak of human memories rather than of human memory, since people have several distinctly different types. The human memory and cognition lab uses empirical, computational, and developmental approaches to understand how memory works in humans. Turn the cards, test your memory see whether you can match them in correct pairs. instructions to play. there are pairs of images hidden in this applet. Try remember where the images are f youre about to go crazy pictures in this game are from poisons icons. return to game index. How is your memory for faces? back to games back to memory games back to face recognition. ame developed by kien caoxuan. When you are considering face, try to imprint it ...
In the late 1990s, I was doing a postdoc at the wonderful DNAX Research Institute of Molecular and Cellular Biology in California. Many fundamental discoveries in basic molecular and cellular immunology had been made at DNAX in the 1980s and 90s. To be a young postdoc from Sydney working in that environment was just magic, inspirational, and influential. Toward the end of my postdoc, I was starting to think about returning to Australia for the next phase of my career. Luckily for me, in 1998, I published my first ever paper in JEM (Tangye et al., 1998). This paper identified specific cell surface markers (particularly CD27) that enabled detection (and subsequent isolation and detailed functional analysis) of human memory B cells. I honestly think that having achieved some measure of success during my DNAX postdoc-i.e., a first author JEM paper-played a very important role in my securing a research fellowship awarded by the University of Sydney, which enabled me to return to a position in Sydney ...
Regulation of inhibitory IgSF receptors in memory B cells by IL-4. Naive and memory B cells were purified from peripheral blood and cultured either in medium al
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Tálamus je siva možganovina jajčaste somerne[1] oblike v zadajšnjem delu medmožganov (diencefalona) s številnimi jedri.[2] Pomemben je pri prevajanju senzoričnih in motoričnih signalov v možgansko skorjo[1][3] ter uravnavanju zavesti, spanja in budnosti. Talamus predstavlja del kompleksne strukture možganskih jeder hipotalamusa, epitalamusa, pretalamusa (ventralnega talamusa) in dorzalnega talamusa.[4] ...
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The regulatory cyclin, Cyclin T1 (CycT1), is a host factor essential for HIV-1 replication in CD4 T cells and macrophages. The importance of CycT1 and the Positive Transcription Elongation Factor b (P-TEFb) complex for HIV replication is well-established, but regulation of CycT1 expression and protein levels during HIV replication and latency establishment in CD4 T cells is less characterized. To better define the regulation of CycT1 levels during HIV replication in CD4 T cells, multiparameter flow cytometry was utilized to study the interaction between HIV replication (intracellular p24) and CycT1 of human peripheral blood memory CD4 T cells infected with HIV in vitro. CycT1 was further examined in CD4 T cells of human lymph nodes. In activated (CD3+CD28 costimulation) uninfected blood memory CD4 T cells, CycT1 was most significantly upregulated in maximally activated (CD69+CD25+ and HLA.DR+CD38+) cells. In memory CD4 T cells infected with HIV in vitro, two distinct infected populations of p24+CycT1+
Given the genetic diversity of B-cell lymphomas and differential antigen expression patterns across lymphoma subtypes, it is unlikely that a single small molecule or antibody-based therapeutic will effectively treat all categories of NHL. Therefore, the use of therapeutic antibody combinations targeting different tumor antigens is expected to produce a more robust antitumor response. Simultaneously targeting CD20 and the TNFR family member CD40 may be productive, because both are expressed on the majority of B-cell lymphomas and mediate differential signaling events through their cytoplasmic domains. We evaluated the potential of improving rituximab-based therapies in NHL by targeting CD40 with dacetuzumab. In vivo analysis of the dacetuzumab-rituximab combination in the Ramos NHL xenograft model showed the capacity of dacetuzumab to augment rituximab activity. Potential mechanisms of action behind the ability of dacetuzumab to enhance rituximab activity in vivo include improved recruitment of ...
Issuu is a digital publishing platform that makes it simple to publish magazines, catalogs, newspapers, books, and more online. Easily share your publications and get them in front of Issuus millions of monthly readers. Title: Multiple trace theory of human memory, Author: Daniel Krchnak, Name: Multiple trace theory of human memory, Length: 17 pages, Page: 1, Published: 2013-09-23
Siva Athreya speaking at BIRS workshop, Stochastic Analysis and its Applications, on Tuesday, October 24, 2017 on the topic: Small noise limit for singularly perturbed diffusion.
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One possibility, however, was that IgM+ memory cells switched immunoglobulin isotype after challenge and contributed to the swIg+ progeny. This possibility was difficult to assess as long as swIg+ memory cells were present at the time of challenge. Therefore, the secondary response was tested in mice that were primed with PE 450 days earlier and contained 100,000 PE-specific IgM+ and scarcely any swIg+ memory cells. These mice generated very few swIg+ cells of any kind after challenge, which indicated that the IgM+ memory cells did not undergo isotype switching. The IgM+ memory cells increased only twofold after challenge (Fig. 4B), in contrast to the robust primary response of naïve IgM+ cells to intraperitoneal injection of PE, which generated many IgM+ and swIg+ germinal center and memory cells (Fig. 4C).. Several lines of evidence suggested that the poor secondary response of IgM+ memory cells was related to anti-PE immunoglobulin present during challenge. Injection of hyperimmune serum ...
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Purified Recombinant Human SIVA1 Protein, Myc/DDK-tagged, C13 and N15-labeled from Creative Biomart. Recombinant Human SIVA1 Protein, Myc/DDK-tagged, C13 and N15-labeled can be used for research.
View mouse Siva1 Chr12:112644828-112649152 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression
... pathway induced by the CD27 antigen, a member of the tumor necrosis factor receptor (TFNR) superfamily. The CD27 antigen ... Prasad KV, Ao Z, Yoon Y, Wu MX, Rizk M, Jacquot S, Schlossman SF (Jul 1997). "CD27, a member of the tumor necrosis factor ... Held-Feindt J, Mentlein R (2002). "CD70/CD27 ligand, a member of the TNF family, is expressed in human brain tumors". Int. J. ... SIVA1 has been shown to interact with CD27. Siva protein is a zinc-containing intracellular ligand of the CD4 receptor that ...
... naive B cells are CD27-, memory B-cells are CD27+ and plasma cells are CD27++. The surface antigen CD138 (syndecan-1) is ... Another important surface antigen is CD319 (SLAMF7). This antigen is expressed at high levels on normal human plasma cells. It ... After leaving the bone marrow, the B cell acts as an antigen-presenting cell (APC) and internalizes offending antigens, which ... Pieces of the antigen (which are now known as antigenic peptides) are loaded onto MHC II molecules, and presented on its ...
CD27: This molecule supports antigen-specific expansion of naïve T cells and is vital for the generation of T cell memory. CD27 ... CD27's activity is governed by the transient availability of its ligand, CD70, on lymphocytes and dendritic cells. CD27 ... "Agonist anti-human CD27 monoclonal antibody induces T cell activation and tumor immunity in human CD27-transgenic mice". J ... Like CD27, OX40 promotes the expansion of effector and memory T cells, however it is also noted for its ability to suppress the ...
However, they are able to promote the secretion of immunoglobulins though CD27/CD70 interactions. B cells begin expressing CD27 ... Centroblasts do not express immunoglobulins and are unable to respond to the follicular dendritic cell antigens present in the ... CD27 is an important marker for germinal center formation in the lymphoid follicle and is produced by centroblasts interacting ... Xiao, Yanling; Hendriks, Jenny; Langerak, Petra; Jacobs, Heinz; Borst, Jannie (2004-06-15). "CD27 Is Acquired by Primed B Cells ...
In a secondary response, the memory B cells specific to the antigen or similar antigens will respond. When memory B cells ... Memory B cells are typically distinguished by the cell surface marker CD27, although some subsets do not express CD27. Memory B ... where they can be activated by a floating foreign peptide brought in through the lymph or by antigen presented by antigen ... When reintroduced to antigen, some of these B1 cells can differentiate into memory B cells without interacting with a T cell. ...
In humans, CD27 is a good marker for plasma cells, naive B cells are CD27-, memory B-cells are CD27+ and plasma cells are ... Surface antigens[edit]. Terminally differentiated plasma cells express relatively few surface antigens, and do not express ... Another important surface antigen is CD319 (SLAMF7). This antigen is expressed at high levels on normal human plasma cells. It ... After leaving the bone marrow, the B cell acts as an antigen presenting cell (APC) and internalizes offending antigens, which ...
... antigens, cd24 MeSH D23.050.301.264.035.126 - antigens, cd26 MeSH D23.050.301.264.035.127 - antigens, cd27 MeSH D23.050.301.264 ... antigens, cd18 MeSH D23.050.301.264.894.126 - antigens, cd26 MeSH D23.050.301.264.894.127 - antigens, cd27 MeSH D23.050.301.264 ... antigens, cd24 MeSH D23. - antigens, cd26 MeSH D23. - antigens, cd27 MeSH D23. - ... antigens, cd18 MeSH D23.101.100.894.126 - antigens, cd26 MeSH D23.101.100.894.127 - antigens, cd27 MeSH D23.101.100.894.128 - ...
MZ B cells shuttle between the blood-filled marginal zone for antigen collection and the follicle for antigen delivery to ... They are furthermore distinguished by the expression of CD9 and CD27 (in humans). In mice, MZ B cells characteristically ... MZ B cells respond to a wide spectrum of T-independent, but also T-dependent antigens. It is believed that MZ B cells are ... Moreover, MZ B cells are potent antigen-presenting cells, that are able to activate CD4+ T cells more effectively than FO B ...
... +Antigens at the US National Library of Medicine Medical Subject Headings (MeSH) Human CD27 genome location and CD27 gene ... "Entrez Gene: CD27 CD27 molecule". Ribot JC, deBarros A, Pang DJ, Neves JF, Peperzak V, Roberts SJ, et al. (April 2009). "CD27 ... CD27 has been shown to interact with SIVA1, TRAF2 and TRAF3. Some mutations can decrease the expression of CD27. Three such ... CD27 is expressed on both naïve and activated effector T cells as well as NK cells and activated B cells. It is a type I ...
B1b cells seem to recognize more types of antigens including intracellular antigens. Previously, B1b cell antigen recognition ... Human B1 cells have been found to have marker profile of CD20+CD27+CD43+CD70- and could either be CD5+ or CD5-, which has been ... making antibodies against antigens and acting as antigen-presenting cells. These B1 cells are commonly found in peripheral ... Hence, there appears to be a role for self or foreign antigen in shaping the repertoire of the B-1 B cell compartment. B1 B ...
... and malignant cells that express activation-induced cytidine deaminase but not CD27, CCL20, MAdCAM-1, or C-C chemokine receptor ... develops as a consequence of chronic inflammation and specific antigen stimulation. In support of this possibility, there have ... and associations with chronic inflammatory diseases and chronic antigen stimulation; Mantle cell lymphoma differs from DFL by ...
Signaling domains from a wide variety of co-stimulatory molecules have been successfully tested, including CD28, CD27, CD134 ( ... Identification of good antigens has been challenging: such antigens must be highly expressed on the majority of cancer cells, ... After an antigen is bound to the external antigen recognition domain, CAR receptors cluster together and transmit an activation ... T cells are genetically engineered to express chimeric antigen receptors specifically directed toward antigens on a patient's ...
Antigen-specific peripheral shaping of the natural regulatory T cell population. „J Exp Med". 205 (13), s. 3105-3117, grudzień ... Epithelial and dendritic cells in the thymic medulla promote CD4+Foxp3+ regulatory T cell development via the CD27-CD70 pathway ... De novo production of antigen-specific suppressor cells in vivo. „Nat Protoc". 1 (2), s. 653-661, 2006. PMID: 17802642. ... Regulation of the immune response to tumor antigens. X. Activation of third-order suppressor T cells that abrogate anti-tumor ...
Among them, it is associated with a particular genetic variant of the human leukocyte antigen called HLA-DR7. This variant is ... peripheral B-cells show more functional IgD+ positive CD27 cells than usual.[citation needed] In the followup of 111 patients, ...
Gene Signature : CCL5, CD27, CD274 (PD-L1), CD276 (B7-H3), CD8A, CMKLR1, CXCL9, CXCR6, HLA-DQA1, HLA-DRB1, HLA-E, IDO1, LAG3, ... Antigen-activated T cells secrete CCR5 ligands (CCL2 and CCL3) to recruit natural killer (NK) cells and other innate immune ... They are found to express conformational epitopes, such as MHC molecules, as nonself antigens, which activates both B and T ... by antigen-presenting cells (APCs). CXCR3 expressing Th1-polarized CD4 T cells and cytotoxic T cells are recruited to the site ...
And there was an increase in the expression of co-stimulatory molecules CD27 and CD28 (CD27+CD28+, p=0.016; and CD27-CD28+, p= ... The investigators for this work used influenza matrix protein antigen and the tumor antigens Melan-A/MART-1 and survivin to ... In the absence of antigen presentation via MHC class II molecules, efti reactivates dormant antigen-experienced memory T cells ... circulating tumor antigen), and efti increases activation of antigen-presenting cells (APCs) as they take up that debris. This ...
In a secondary response, the memory B cells specific to the antigen or similar antigens will respond.[2] When memory B cells ... Memory B cells are typically distinguished by the cell surface marker CD27, although some subsets do not express CD27. Memory B ... When reintroduced to antigen, some of these B1 cells can differentiate into memory B cells without interacting with a T cell.[7 ... If the memory B cell later encounters the same antigen, it triggers an accelerated and robust secondary immune response.[1][2] ...
This antigen along with other blood group antigens was used to identify the Basque people as a genetically separate group.[49] ... Because the Duffy antigen is uncommon in those of Black African descent, the presence of this antigen has been used to detect ... The Fy4 antigen, originally described on Fy (a-b-) RBCs, is now thought to be a distinct, unrelated antigen and is no longer ... The Duffy antigen is expressed in greater quantities on reticulocytes than on mature erythrocytes.[21] While the Duffy antigen ...
... chimeric antigen receptor T cell therapy using CD19-directed CAR-T cells; and lenalidomide, a drug with multiple anti-tumor ... a monoclonal antibody that binds to the CD27 protein expressed by cells and thereby promotes the anti-tumor activity of T-cells ...
... antigen is a protein that in humans is encoded by the CD160 gene. CD160 is a 27 kDa glycoprotein which was initially ... CD28-CD27-cells. In tissues, CD160 is expressed on all intestinal intraepithelial lymphocytes. CD160 shows a broad specificity ... CD160+Antigen at the US National Library of Medicine Medical Subject Headings (MeSH) Human CD160 genome location and CD160 gene ...
CD27 plays a role in lymphoid proliferation, differentiation, and apoptosis. The acquisition of CD27 and Flt3 by the HSC ... Once in a secondary lymphoid organ the B cell can be introduced to an antigen that it is able to recognize. Through this ... It can be expressed as a set (Lin2, Sca1high, c-kit high, CD44+, Thy1.1low, CD27 2, and IL-7Ra2). This set is a 'barcode' for ... T cells are formed in bone marrow then migrate to the cortex of the thymus to undergo maturation in an antigen-free environment ...
... is a ligand for CD27. The CD70 protein is expressed on highly activated lymphocytes (like in T- and B-cell lymphomas). It ... Seattle Genetics Third Quarter 2013 Financial Report[permanent dead link] CD70+Antigens at the US National Library of Medicine ...
CD25 deficiency CD27 deficiency STAT5b deficiency ITK deficiency SH2D1A deficiency (XLP1) MAGT1 deficiency DOCK2 deficiency ... selective immunoglobulin A deficiency Specific antibody deficiency to specific antigens with normal B cell and normal Ig ...
... antigens, cd27 MeSH D12.776.543.750.705.852.760.072 - antigens, cd30 MeSH D12.776.543.750.705.852.760.097 - antigens, cd40 MeSH ... antigen, b-cell MeSH D12.776.543.750.705.816.821.500 - antigens, cd79 MeSH D12.776.543.750.705.816.824 - receptors, antigen, t- ... antigens, cd22 MeSH D12.776.543.550.200.124 - antigens, cd24 MeSH D12.776.543.550.200.131 - antigens, cd31 MeSH D12.776.543.550 ... antigens, cd11a MeSH D12.776.543.750.705.408.100.150 - antigens, cd11b MeSH D12.776.543.750.705.408.100.200 - antigens, cd11c ...
Integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide), also known as ITGAL, is ... Maurer D, Holter W, Majdic O, Fischer GF, Knapp W (1991). "CD27 expression by a distinct subpopulation of human B lymphocytes ... CD11a+Antigen at the US National Library of Medicine Medical Subject Headings (MeSH) ITGAL Info with links in the Cell ... CD11a is one of the two components, along with CD18, which form lymphocyte function-associated antigen-1. Efalizumab acts as an ...
These mutations include those in the following genes: UNC13D, STX11, RAB27A, STXBP2, LYST, PRF1 1, SH2D1A, BIRC4, ITK, CD27, ... This inability to remove infected and antigen-presenting cells and terminate the immune response leads to uncontrolled ...
CD27-/IgD- memory B cells are associated with increased disease activity and renal lupus. T cells, which regulate B-cell ... That leads to maturation of DCs and also to the presentation of intracellular antigens of late apoptotic or secondary necrotic ... In close proximity to TBM, follicular dendritic cells (FDC) are localised in GC, which attach antigen material to their surface ... When apoptotic material is not removed correctly by phagocytes, they are captured instead by antigen-presenting cells, which ...
Memory B cells with increased CD27+/IgD-are less susceptible to immunosuppression. CD27-/IgD- memory B cells are associated ... In close proximity to TBM, follicular dendritic cells (FDC) are localised in GC, which attach antigen material to their surface ... Antinuclear antibody (ANA) testing and anti-extractable nuclear antigen (anti-ENA) form the mainstay of serologic testing for ... That leads to maturation of DCs and also to the presentation of intracellular antigens of late apoptotic or secondary necrotic ...
B-cells that have not encountered an antigen are called naive B cells. When naïve B-cells encounter an antigen, one of the ... In normal lymphoid tissues CD27 and its ligand CD70 have a restricted expression pattern, but a 1999 study found CD70 on 71% of ... that bind to a specific antigen. Once activated by an antigen, B-cells proliferate and further differentiate into plasma cells ... Follicular dendritic cells and T cells help to select the B-cells that have a high affinity to the antigen for further ...
Dong HY, Shahsafaei A, Dorfman DM (2003). "CD148 and CD27 are expressed in B cell lymphomas derived from both memory and naïve ... Tissue Antigens. 54 (5): 485-98. doi:10.1034/j.1399-0039.1999.540506.x. PMID 10599888. Billard C, Delaire S, Raffoux E, ...
CD27 • CD28 • CD29 • CD30 • CD31 • CD32 (A, B) • CD33 • CD34 • CD35 • CD36 • CD37 • CD38 • CD39 • CD40 • CD41 • CD42 (a, b, c, ... 2000). "Characterization of a new member of the TNF family expressed on antigen presenting cells.". Biol. Chem. 380 (12): 1443- ... "BLyS receptor signatures resolve homeostatically independent compartments among naïve and antigen-experienced B cells.". Semin ...
I. Partial characterization of soluble Ki-1 antigen and detection of the antigen in cell culture supernatants and in serum by ... Josimovic-Alasevic O, Dürkop H, Schwarting R, Backé E, Stein H, Diamantstein T (Jan 1989). "Ki-1 (CD30) antigen is released by ... CD30+Antigens at the US National Library of Medicine Medical Subject Headings (MeSH) ... results from cDNA cloning and sequence comparison of the CD30 antigen from different sources". Molecular Immunology. 31 (17): ...
Macrophage-1 antigen (CD11b+CD18). *VLA-4 (CD49d+CD29). *Glycoprotein IIb/IIIa (ITGA2B+ITGB3) ...
Seligman P. A., Butler C. D., Massey E. J., etal. The p97 antigen is mapped to the q24-qter region of chromosome 3; the same ... Le Beau M. M., Diaz M. O., Plowman G. D., etal. Chromosomal sublocalization of the human p97 melanoma antigen. (англ.) // Hum. ... Plowman G. D., Brown J. P., Enns C. A., etal. Assignment of the gene for human melanoma-associated antigen p97 to chromosome 3 ... Rose T. M., Plowman G. D., Teplow D. B., etal. Primary structure of the human melanoma-associated antigen p97 ( ...
Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) also known as CD66e (Cluster of Differentiation 66e), is a ... 2001). "Heterogeneous RNA-binding protein M4 is a receptor for carcinoembryonic antigen in Kupffer cells". J. Biol. Chem. 276 ( ... CEACAM5, CD66e, CEA, carcinoembryonic antigen related cell adhesion molecule 5. External IDs. HomoloGene: 128801 GeneCards: ... Oikawa S, Nakazato H, Kosaki G (1987). "Primary structure of human carcinoembryonic antigen (CEA) deduced from cDNA sequence". ...
The protein also carries the Jr(a) antigen, which defines the Junior blood group system.[9] ...
van Rhenen A., van Dongen G. A., Kelder A., et al. The novel AML stem cell associated antigen CLL-1 aids in discrimination ...
"Interaction of glycogen synthase kinase 3beta with the DF3/MUC1 carcinoma-associated antigen and beta-catenin". Molecular and ...
Ebert LM, McColl SR (2002). "Up-regulation of CCR5 and CCR6 on distinct subpopulations of antigen-activated CD4+ T lymphocytes ... This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may ... dendritic cells induce antitumor immunity when genetically fused with nonimmunogenic tumor antigens". J. Immunol. 167 (11): ...
It is also called Lewis x and SSEA-1 (stage-specific embryonic antigen 1) and represents a marker for murine pluripotent stem ... CD15 Antigen at the US National Library of Medicine Medical Subject Headings (MeSH) ... CD15 (3-fucosyl-N-acetyl-lactosamine) is a cluster of differentiation antigen - an immunologically significant molecule. CD15 ...
A new ligand for human leukocyte antigen class II antigens". The Journal of Experimental Medicine. 176 (2): 327-37. doi:10.1084 ... A new ligand for human leukocyte antigen class II antigens". The Journal of Experimental Medicine. 176 (2): 327-37. doi:10.1084 ... antigen processing and presentation of exogenous peptide antigen via MHC class II. ... antigen binding. • transmembrane signaling receptor activity. • MHC class II protein binding. Cellular component. • membrane. • ...
CD27 • CD28 • CD29 • CD30 • CD31 • CD32 (A, B) • CD33 • CD34 • CD35 • CD36 • CD37 • CD38 • CD39 • CD40 • CD41 • CD42 (a, b, c, ... CD97 antigen je protein koji je kod ljudi kodiran CD97 genom.[1][2][3] ... 2001). "Tissue distribution of the human CD97 EGF-TM7 receptor". Tissue Antigens 57 (4): 325-31. PMID 11380941. doi:10.1034/j. ... "Expression cloning and chromosomal mapping of the leukocyte activation antigen CD97, a new seven-span transmembrane molecule of ...
Autoreactive thymic B cells are efficient antigen-presenting cells of cognate self-antigens for T cell negative selection., 110 ... Foxp3+ regulatory T cell development via the CD27-CD70 pathway, J Exp Med., 8. aprill 2013; 210(4): 715-728., doi: 10.1084/jem. ... Ana C. Anderson ja Vijay K. Kuchroo, Expression of Self-antigen in the Thymus A Little Goes a Long Way, 1. detsember 2003 // ... Christian Koble ja Bruno Kyewski, The thymic medulla: a unique microenvironment for intercellular self-antigen transfer, J. Exp ...
"Entrez Gene: ITGB3 integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)".. *^ May, K. E.; Villar, J.; Kirtley, S.; ... CD61+Antigens at the US National Library of Medicine Medical Subject Headings (MeSH) ...
"Direct association of adenosine deaminase with a T cell activation antigen, CD26". Science. 261 (5120): 466-9. doi:10.1126/ ...
B cells can present antigens to a specialized group of helper T cells called TFH cells. If an activated TFH cell recognizes the ... Roles of T cell-B-cell-activating molecule (5c8 antigen) and CD40 in contact-dependent help". Journal of Immunology. 149 (12): ... It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In ... Grewal, IS; Xu, J; Flavell, RA (7 December 1995). "Impairment of antigen-specific T-cell priming in mice lacking CD40 ligand". ...
antigen processing and presentation of peptide antigen via MHC class I. • antigen processing and presentation of exogenous ... antigen processing and presentation of exogenous peptide antigen via MHC class I. • lipoprotein transport. • negative ... peptide antigen via MHC class I, TAP-dependent. • platelet degranulation. • MyD88-dependent toll-like receptor signaling ...
Primarily, the VCAM-1 protein is an endothelial ligand for VLA-4 (Very Late Antigen-4 or integrin α4β1) of the β1 subfamily of ...
... uveitis antigens induce CXCR3- and CXCR5-expressing lymphocytes and immature dendritic cells to migrate (англ.) // Blood (англ ...
In addition to aiding with cytotoxic T cell antigen interactions the CD8 co-receptor also plays a role in T cell signaling. The ... the CD8 co-receptor plays a role in T cell signaling and aiding with cytotoxic T cell antigen interactions. ... This affinity keeps the T cell receptor of the cytotoxic T cell and the target cell bound closely together during antigen- ... Once the T cell receptor binds its specific antigen Lck phosphorylates the cytoplasmic CD3 and ζ-chains of the TCR complex ...
Iron-deficiency anemia (IDA) may be the only symptom for CD,[26] detected in subclinical CD[27] and is accompanied by a ... Al-Toma A, Goerres MS, Meijer JW, Peña AS, Crusius JB, Mulder CJ (2006). "Human leukocyte antigen-DQ2 homozygosity and the ...
antigen binding. • virus receptor activity. • protein binding. • transmembrane signaling receptor activity. • identical protein ...
CD27 • CD28 • CD29 • CD30 • CD31 • CD32 (A, B) • CD33 • CD34 • CD35 • CD36 • CD37 • CD38 • CD39 • CD40 • CD41 • CD42 (a, b, c, ... 1996). "CD88 antibodies specifically bind to C5aR on dermal CD117+ and CD14+ cells and react with a desmosomal antigen in human ...
CD74 (англ. HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain) - мембранный белок ... II histocompatibility antigen gamma chaingamma chain of class II antigensIiHLA-DR antigens-associated invariant chainIa antigen ... Riberdy J.M., Newcomb J.R., Surman M.J., Barbosa J.A., Cresswell P. HLA-DR molecules from an antigen-processing mutant cell ... Machamer C.E., Cresswell P. Biosynthesis and glycosylation of the invariant chain associated with HLA-DR antigens (англ.) // ...
CD27 and CD30, besides the ligands on which the family is named (TNF). ... "Delayed hypersensitivity in vitro: its mediation by cell-free substances formed by lymphoid cell-antigen interaction". Proc. ...
... can be found in Genscripts Peptide Antigen Database. Anti- CD27 antigen; pAb has guaranteed Elisa titer of 1:64000 and 95% WB ... Protein name and sequence of (CD27 antigen) Protein Names. Recommended name:. CD27 antigen ... What we offer for CD27 antigen. Learn more about PolyExpress™ custom pAb production services ... Cd27. Protein Sequence. SEQUENCE 250 AA; 28164 MW; 48477C2E0C244697 CRC64; MAWPPPYWLC MLGTLVGLSA TLAPNSCPDK HYWTGGGLCC ...
sp,P26842,CD27_HUMAN CD27 antigen OS=Homo sapiens OX=9606 GN=CD27 PE=1 SV=2 ... "The T cell activation antigen CD27 is a member of the nerve growth factor/tumor necrosis factor receptor gene family.". ... "The TNF receptor family member CD27 signals to Jun N-terminal kinase via Traf-2.". Gravestein L.A., Amsen D., Boes M., Calvo C. ... CD27_HUMAN. ,p>This subsection of the Entry information section provides one or more accession number(s). These are stable ...
CD27 Promotes CD4 Effector T Cell Survival in Response to Tissue Self-Antigen.. Authors:. Kelly A Remedios Lauren Meyer Bahar ... Paper: CD27 Promotes CD4 Effector T Cell Survival in Response to Tissue Self-Antigen. ... Expression of CD27 on Ag-specific Teff cells resulted in enhanced skin inflammation when compared with CD27-deficient Teff ... Using BH3 profiling to assess mitochondrial cell priming, we found that CD27-deficient cells were equally as sensitive as CD27- ...
CD27- group (n = 93) had higher level of plasma cell proportion (37.00% vs 22.50%, p , .05), β2-MG (5.42 vs 3.20 mg/L, p , .05 ... p,To investigate the role of CD27 in multiple myeloma(MM), bone marrow samples from 165 newly diagnosed MM were analysed by ... CD27 antigen negative expression indicates poor prognosis in newly diagnosed multiple myeloma Bin Chu 1 , Li Bao 2 , Yutong ... CD27 antigen negative expression indicates poor prognosis in newly diagnosed multiple myeloma Bin Chu et al. Clin Immunol. 2020 ...
Compare CD27 ELISA Kits from CUSABIO Technology LLC from leading suppliers on Biocompare. View specifications, prices, ... The ELISA (enzyme-linked immunosorbent assay) is a widely used application for detecting and quantifying proteins and antigens ... CD27 ELISA Kits from CUSABIO Technology LLC. ...
p,CD27 (TNFRSF7) is a member of the TNFR family. It is a type I membrane glycoprotein with a MW of 55KDa that forms a disulfide ... CD27L receptor, T-cell activation antigen CD27, Tumor necrosis factor receptor superfamily member 7 Ave. Rating Submit a Review ... T cells expressing CD27 and CD70 play a key role in regulating B-cell activation and immunoglobulin synthesis. Soluble CD27 ( ... Antigen References 1. Camerini D, et al. 1991 J. Immunol. 147:3165.. 2. Prasad KV, et al. 1997. Proc. Natl. Acad. Sci. 94:6346. ...
CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70 and ... CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. It is a lymphocyte-specific member of the TNF-receptor ... Antigen References 1. Hintzen R, et al. 1994. Immunol. Today 15:307.. 2. Agematsu K, et al. 1995. J. Immunol. 154:3627. ... CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70 and ...
Shop a large selection of products and learn more about CD27 Armenian Hamster anti-Human, Mouse, Rat, PE, Clone: LG.7F9, ... CD27 antigen, CD27L receptor, T cell activation antigen CD27, T cell activation antigen S152, T-cell activation antigen CD27, ... degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a ... CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 has ...
The hexavalent agonist significantly boosted antigen-specific T cell responses while having no effect on non-specific T cells ... Agonistic stimulation of CD27 is therefore a promising therapeutic concept in immuno-oncology intended to boost and sustain T ... Agonistic stimulation of CD27 is therefore a promising therapeutic concept in immuno-oncology intended to boost and sustain T ... In this manuscript, we describe the development of HERA-CD27L, a true CD27 agonist with a clearly defined forward-signaling ...
A modulating disulfide-linked T cell activation antigen. is an eagle-i resource of type Journal article at Oregon Health & ... S152 (CD27). A modulating disulfide-linked T cell activation antigen.. eagle-i ID. ...
Persistence of EBV antigen-specific CD8 T cell clonotypes during homeostatic immune reconstitution in cancer patients.. PLoS ... CD27 and Chronic Lymphocytic Leukemia. View Publications. 11. Multiple Myeloma. CD27 and Multiple Myeloma. View Publications. ... CD27. COSMIC, Sanger Institute. Somatic mutation information and related details CD27. GEO Profiles, NCBI. Search the gene ... CD70-dependent release of soluble CD27 in cocultures may explain the high CD27 levels observed in sera of patients with CD70- ...
We also examined the mechanism of survival signaling by CD27 using primary DCs as antigen-presenting cells. Conventional DCs ... Mice. WT, Cd27-/- (21), OT-I (45), OT-I;Cd27-/-, F5 (46), and F5;Cd27-/- mice on a C57BL/6 background were used for experiments ... CD27 promotes IL-2 expression in effector CD8+ T cells upon influenza virus infection. Cd27+/- and Cd27-/- littermates were ... To examine whether CD27 promoted IL-2 expression during an immune response in vivo, Cd27+/- (control) and Cd27-/- littermates ...
Cd27 CD27 antigen MGI:88326 .yui-skin-sam .yui-dt th{ background:url( ...
Antigens, CD / blood* * Antigens, Differentiation, Myelomonocytic / blood* * Antiretroviral Therapy, Highly Active* * ... Soluble CD14, CD163, and CD27 biomarkers distinguish ART-suppressed youth living with HIV from healthy controls J Leukoc Biol. ... projection to assess single biomarkers that maximally contribute to discriminate among outcome groups identified soluble CD27, ...
... mice and found that CD27 makes essential contributions to mature CD4+ and CD8+ T cell function: CD27 supported antigen-specific ... The Traf-linked tumor necrosis factor receptor family member CD27 is known as a T cell costimulatory molecule. We generated ... We generated CD27-/- mice and found that CD27 makes essential contributions to mature CD4+ and CD8+ T cell function: CD27 ... CD27 is required for generation and long-term maintenance of T cell immunity Nat Immunol. 2000 Nov;1(5):433-40. doi: 10.1038/ ...
Genetic association study of interferon lambda 3, CD27, and human leukocyte antigen-DPB1 with dengue severity in Thailand ... Genetic association study of interferon lambda 3, CD27, and human leukocyte antigen-DPB1 with dengue severity in Thailand ... CD27, and human leukocyte antigen-DPB1 (HLA-DPB1) genes in Thai dengue patients. ... CD27, and HLA-DPB1 was selected to be analyzed. Genotyping was performed by TaqMan real-time PCR assay, and the association ...
Diagnostic Value of CD27 Antigen in Patients with Multiple Myeloma Diagnostic Value of CD27 Antigen in Patients with Multiple ... p,,b,OBJECTIVE,/b,To explore the expression of CD27 antigen in patients with multiple myeloma(MM), and its clinical diagnostic ... Laboratory examination of 58 cases of CD27 negative and 49 cases of CD27 positive MM patients indicated that no significant ... and IGH rearrangement results all were not significantly different between 17 cases of CD27 negative and 17 cases of CD27 ...
Armenian hamster monoclonal CD27 antibody [LG.3A10] validated for IP, IHC, Flow Cyt and tested in Human, Mouse and Rat. ... T cell activation antigen CD27 antibody. *T cell antivation antigen S152 antibody ...
500318 Cd27; CD27 antigen precursor 25069 Tnfrsf8; tumor necrosis factor receptor superfamily member 8 precursor 171369 Cd40; ... MHC class I antigen K06751 MHC1; MHC class I antigen K06751 MHC1; MHC class I antigen K06751 MHC1; MHC class I antigen K06751 ... MHC class I antigen K06751 MHC1; MHC class I antigen K06751 MHC1; MHC class I antigen K06751 MHC1; MHC class I antigen K06751 ... MHC class I antigen K06751 MHC1; MHC class I antigen K06751 MHC1; MHC class I antigen K06751 MHC1; MHC class I antigen K06751 ...
Jetzt diesen anti-CD27 Antikörper bestellen. , Produkt ABIN4260283 ... Armenischer Hamster Monoklonal CD27 Antikörper für FACS, IHC, IHC (p). ... Antigen CD27 Molecule (CD27) Synonyme für dieses Antigen anzeigen * TNFRSF7 * S152 * Tnfrsf7 ... anti-CD27 Antikörper (CD27 Molecule) (DyLight 405) CD27 Antikörper (CD27 Molecule) (DyLight 405). Details for Product anti-CD27 ...
Jetzt diesen anti-CD27 Antikörper bestellen. , Produkt ABIN4260274 ... Armenischer Hamster Monoklonal CD27 Antikörper für FACS, IHC, IHC (p). ... Antigen CD27 Molecule (CD27) Synonyme für dieses Antigen anzeigen * TNFRSF7 * S152 * Tnfrsf7 ... anti-CD27 Antikörper (CD27 Molecule) (FITC) CD27 Antikörper (CD27 Molecule) (FITC). Details for Product anti-CD27 Antibody No. ...
T cell populations seen in association with prolonged interactions between CD27 and its ligand CD70 (27). Because CD27-CD70 ... 5⇑B). Larger numbers of CD27+ p18-specific CD8+ T cells were also observed in the spleens of the mice having depot Ag for a ... Antigen persistence is required throughout the expansion phase of a CD4+ T cell response. J. Exp. Med. 201: 1555-1565. ... A, Expression of CD62L, CD27, and CD127 on CD8+tetramer+ T cells in the PBMCs of the immunized mice. Data are presented as the ...
Invitrogen Anti-CD27 Monoclonal (LG.7F9), eBioscience , Catalog # 62-0271-82. Tested in Flow Cytometry (Flow) applications. ... Protein Aliases: CD antigen 27; CD27; CD27 antigen; CD27L receptor; sCD27; soluble CD27; T-cell activation antigen CD27; Tumor ... degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a ... CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 has ...
Invitrogen Anti-CD27 Monoclonal (O323), eBioscience™, Catalog # 56-0279-42. Tested in Flow Cytometry (Flow) applications. This ... CD27 antigen; CD27L receptor; T cell activation antigen CD27; T cell activation antigen S152; T-cell activation antigen CD27; ... degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a ... Published figure using CD27 monoclonal antibody (Product # 56-0279-42). Published figure using CD27 monoclonal antibody ( ...
The reduced proportion of CD27-IgD- B cells in blood and the increased proportion in the gut implies that CD27-IgD- B cells are ... CD27-IgD- B cells have been implicated in immune responses to intestinal bacteria and recruitment to GALT, and may contribute ... We analyzed the frequencies of known B cell subsets in blood and observed a consistent reduction in the proportion of CD27−IgD ... By mass cytometry we demonstrated that CD27−IgD− B cells were proportionately enriched in the gut-associated lymphoid tissue ( ...
Gene name: CD70 antigen (CD27 ligand). Contains complete coding sequence?: Y. Chromosome: 19; Localization: 19 p13. Tissue: B- ... Molecular and biological characterization of a ligand for CD27 defines a new family of cytokines with homology to tumor ... The cloning of CD70 and its identification as the ligand for CD27. J. Immunol. 152: 1756-1761, 1994. PubMed: 8120384 ... Gene product: CD70 antigen (CD27 ligand) [CD70]. Target Gene: CD70 antigen (CD27 ligand) ...
This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It ... This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It ... Suggested Antigen Peptide Sequences for CD70 Gene. GenScript: Design optimal peptide antigens:. *cDNA FLJ60770, highly similar ... View all 16 R&D Systems CD70 (CD27 Ligand/TNFSF7) Products. *View all R&D Systems CD70 (CD27 Ligand/TNFSF7) Proteins and ...
Unconjugated Whole IgG Rabbit anti-CD27 Antibody, Affinity Purified suitable for WB applications. Visit for all your ... T cell activation antigen S152 antibody. *T14 antibody. *T-cell activation antigen CD27 antibody ...
CD45RA+ T lymphocytes respond by upregulating the CD27 antigen. After maximal stimulation, the CD27 antigen cannot be re- ... CD45RA+ T lymphocytes respond by upregulating the CD27 antigen. After maximal stimulation, the CD27 antigen cannot be re- ... Present on most peripheral blood T lymphocytes and medullary thymocytes, the CD27 antigen is upregulated upon activation with ... Present on most peripheral blood T lymphocytes and medullary thymocytes, the CD27 antigen is upregulated upon activation with ...
CD27 is expressed on subsets of B, T, and NK cells. CD27 binds to CD70. This interaction regulates cell activation. ... Clone LG.3A10 recognizes the human and murine CD27 antigen, a member of the tumor necrosis factor receptor family. ... Clone LG.3A10 recognizes the human and murine CD27 antigen, a member of the tumor necrosis factor receptor family. CD27 is ... Distribution of antigen: B cells, NK cells, plasma cells, red blood cells, T cells, thymocytes ...
  • The interaction between CD27 and its ligand, CD70, plays a role in providing co-stimulation for prolonged lymphocyte survival, enhanced T-cell proliferation, and memory-cell formation. (
  • In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predomitly confined to lymphocytes. (
  • RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. (
  • Tumor necrosis factor receptor superfamily member 7 (TNFRSF7, CD27), expressed primarily by T cells, and its ligand CD27L (TNFSF7, CD70) provide co-stimulatory signals that boost T cell activation, differentiation, and survival. (
  • CD27 signaling, induced by its ligand CD70 (TNFSF7, CD27L), plays an important role in regulating immune responses by providing co-stimulatory signals to boost T cell activation, differentiation, survival and memory formation ( 3 , 6 , 7 ). (
  • The cloning of CD70 and its identification as the ligand for CD27. (
  • Molecular and biological characterization of a ligand for CD27 defines a new family of cytokines with homology to tumor necrosis factor. (
  • This cytokine is a ligand for TNFRSF27/CD27. (
  • Cytokine which is the ligand for CD27. (
  • With its ligand CD70, CD27 acts in a co-stimulatory fashion on T lymphocytes. (
  • There are a few examples of NK receptors that recognize specific antigens, most notably murine Ly49H, which recognizes the virally-encoded ligand m157 ( 11 ). (
  • Via its interactions with ligand CD70, it plays a key role in regulating B-cell activation and immunoglobulin synthesis.CD27 also interacts with the proapoptotic protein SIVA1 and may therefore play a role in apoptosis. (
  • Enrichment of CD27 among centroblasts and the presence of its ligand CD70 on occasional T and B cells in or near germinal centers (GCs) suggested a role for CD27/CD70 interactions in clonal B cell expansion. (
  • CD27 ligand. (
  • 1F5 binds with high affinity and specificity to human and macaque CD27 and competes with ligand binding. (
  • 12 . The method of claim 11 wherein the molecule is selected from Fas ligand and CD27. (
  • and (ii) T cells activated by a third-party antigen, which stimulate B cells in a noncognate fashion via CD40 ligand and cytokine production, here referred to as bystander help ( 2 , 12 ). (
  • Description: The LG.7F9 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. (
  • Various strategies to induce CD27 signaling are currently under investigation and they can be broadly grouped into CD27L-based or agonistic antibody-based approaches ( 4 , 8 - 11 ). (
  • The immunogen for this antibody was CD27. (
  • The following product was used in this experiment: CD27 Monoclonal Antibody (LG.7F9), Super Bright 436, eBioscience from Thermo Fisher Scientific, catalog # 62-0271-82, RRID AB_2734939. (
  • The following antibody was used in this experiment: CD27 Monoclonal Antibody (O323), Alexa Fluor 700, eBioscience™ from Thermo Fisher Scientific, catalog # 56-0279-42, RRID AB_11044789. (
  • The L128 monoclonal antibody specifically binds to human CD27. (
  • In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay. (
  • Cells were incubated with an excess of purified unconjugated CD27 (REA499) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. (
  • In this article, we describe a novel human monoclonal antibody (mAb) specific for CD27 with properties that suggest a potential utility against malignancies that express CD27. (
  • The anti-CD27 mAb 1F5 was effective in mediating antibody-dependent cellular cytotoxicity of human lymphoblastic cell lines and significantly reduced their growth when transplanted into immunodeficient mice. (
  • Antibody diversity is first generated by rearrangement of immunoglobulin (Ig) genes during B cell development in the bone marrow, and later by antigen-driven diversification in germinal centers (GCs). (
  • Rearrangement and assembly of B cell antigen receptor genes generates a diverse antibody repertoire in humans and mice. (
  • Unlike their precursors, they cannot switch antibody classes, cannot act as antigen-presenting cells because they no longer display MHC-II, and do not take up antigen because they no longer display significant quantities of immunoglobulin on the cell surface. (
  • The antibody LT27 reacts with CD27 (T14), a 50-55 kDa type I transmembrane glycoprotein (member of the TNF-receptor superfamily) expressed on medullary thymocytes, peripheral T lymphocytes, some B lymphocytes and NK cells. (
  • Another approach relies upon the use of chimeric antigen receptors (CARs) that confer T cells with the MHC-independent specificity of a tumor antigen-specific antibody and potent T cell activity delivered by TCR and costimulatory domains. (
  • HIV and hepatitis C). Interestingly, the major correlate for sterilizing immunity to both viral and tumor challenge is not antigen-specific antibody titer but rather the number of antigen-specific T cells generated, known as cellular immunity ( 1 ). (
  • Once released into the blood and lymph, these antibody molecules bind to the target antigen (foreign substance) and initiate its neutralization or destruction. (
  • Small volumes of anti-CD27 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. (
  • Varlilumab is a fully human monoclonal antibody that targets CD27, a critical molecule in the activation pathway of lymphocytes. (
  • Moreover, recent findings indicate that DC can present unprocessed antigens to B cells ( 5 , 6 ) and influence the differentiation and survival of antibody-secreting cells (ASC) ( 7 ). (
  • The investigators also looked at the impact of treatment on immune function by measuring antibody generation to antigens found in childhood vaccines. (
  • The authors noted that it was unclear why there would be a discrepancy in the number of patients who had protective titers for the two different antigens, but suggested that there may be differences in mechanisms of antibody production or corticosteroid sensitivity in the response process. (
  • Although antigen boosting leads to a transient increase in specific antibody levels, ongoing polyclonal activation of memory B cells offers a means to maintain serological memory for a human lifetime. (
  • Mouse plasma cells can be long-lived and are able to sustain antibody production for several months in the absence of memory B cells or antigen ( 4 , 5 ). (
  • Yet, if persistence of antigen was the only mechanism available to maintain antibody production, immunological memory would be limited to persisting antigens. (
  • F(ab′) 2 fragments of antibody to human Ig (anti-Ig), used as surrogate antigen, failed to induce B cell proliferation, even in the presence of cytokines ( 15 ). (
  • Sleep on the night after experimental vaccinations against hepatitis A produced a strong and persistent increase in the number of antigen-specific Th cells and antibody titres. (
  • Recombinant rabbit monoclonal antibody raised against full length human CD27. (
  • Rabbit polyclonal antibody raised against a full-length human CD27 protein. (
  • Western Blot analysis of CD27 expression in transfected 293T cell line ( H00000939-T02 ) by CD27 MaxPab polyclonal antibody. (
  • During the year, Roche and Celldex Therapeutics entered into a clinical trial collaboration to evaluate the safety, tolerability and preliminary efficacy of varlilumab, Celldex's CD27 targeting investigational antibody, and MPDL3280A (anti-PDL1), Roche's investigational cancer immunotherapy in a Phase 1/2 study in renal cell carcinoma. (
  • Varlilumab is the firm's fully human monoclonal agonist antibody that binds and activates CD27 , a critical co-stimulatory molecule in the immune activation cascade. (
  • The Traf-linked tumor necrosis factor receptor family member CD27 is known as a T cell costimulatory molecule. (
  • The high expression of CD27, a T-cell costimulatory molecule, on many types of lymphoma and leukemia prompted us to develop human anti-CD27 mAbs and determine their activity against lymphoma cells in vitro and in vivo . (
  • The TNF receptor superfamily member CD27 is a tightly regulated costimulatory molecule activated by ligation through its unique counterreceptor, CD70 ( 3 ). (
  • These T cells bind to the MHC II-antigen molecule and cause activation of the B cell. (
  • CTLA-4 binds to B7 ligands expressed on antigen-presenting cells (APCs) with higher affinity than the costimulatory molecule CD28, and both its gene and surface expression are induced during T cell activation upon APC interaction ( 1 ). (
  • 15. The nucleic acid molecule of claim 1, wherein said nucleic acid molecule comprises a nucleotide sequence encoding a chimeric antigen receptor (CAR), wherein said CAR comprises at least one of said co-stimulatory domains. (
  • CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. (
  • Soluble CD27 (sCD27), the extracellular domain of membrane-bound CD27, can be released after lymphocyte activation by differential splicing of the receptor protein or shedding from the cell surface by metalloproteinases (MMPs). (
  • CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. (
  • CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. (
  • Clone LG.3A10 recognizes the human and murine CD27 antigen, a member of the tumor necrosis factor receptor family. (
  • Whereas adaptive immune cells have rearranged receptor genes to recognize the universe of antigens, natural killer (NK) cells are innate immune lymphocytes with a limited repertoire of germ-line encoded receptors for target recognition. (
  • CD27 antigen is a member of the tumor necrosis factor receptor superfamily, and is needed for generation and maintenance of T cell immunity. (
  • In humans, CD27 is induced by B cell receptor triggering and maintained long-term ( 9 , 10 ). (
  • The TNF receptor superfamily member CD27 is best known for its important role in T-cell immunity but is also recognized as a cell-surface marker on a number of B- and T-cell malignancies. (
  • 1F5 activates T cells only in combination with T-cell receptor stimulation and does not induce proliferation of primary CD27-expressing tumor cells. (
  • These V segment substitutions occur independently of antigen binding to the B cell receptor (BCR), although gut bacteria provide an antigen-independent proliferative stimulus. (
  • To date, there has been no definitive demonstration of antigen-independent postrearrangement diversification in mice, although mouse B cells undergo postrearrangement V gene replacement in the bone marrow in response to interactions with self-antigen, a process referred to as receptor editing ( 10 ). (
  • This gene encodes a protein with an important role in the apoptotic (programmed cell death) pathway induced by the CD27 antigen, a member of the tumor necrosis factor receptor (TFNR) superfamily. (
  • CD27 belongs to the Tumor Necrosis Factor Receptor (TNFR) gene family. (
  • After leaving the bone marrow, the B cell acts as an antigen-presenting cell (APC) and internalizes offending antigens, which are taken up by the B cell through receptor-mediated endocytosis and processed. (
  • We selected most pathways Cd27 participated on our site, such as Cytokine-cytokine receptor interaction, which may be useful for your reference. (
  • Cd27 has several biochemical functions, for example, cysteine-type endopeptidase inhibitor activity involved in apoptotic process, protein binding, transmembrane signaling receptor activity. (
  • This in turn will permit downstream studies of T cell receptor (TCR) isolation, cancer antigen identification and molecular characterization of naturally occurring tumor-reactive T cells in human cancer. (
  • Adoptive transfer of T cells engineered to express a chimeric antigen receptor (CAR) has emerged as a powerful targeted immunotherapy, showing striking responses in highly refractory populations. (
  • Chimeric antigen receptor (CAR)-modified T cells targeting CD19, the best-studied CAR T-cell therapy to date, will be discussed, with a focus on clinical trials for ALL demonstrating efficacy as well as toxicity and toxicity management. (
  • Furthermore, we found that impairment of early signaling events following T cell receptor stimulation because of long term culture allows prediction of costimulatory molecules CD28 and CD27 expression levels and the number of population divisions in culture from a limited subset of signaling proteins. (
  • CD27: Receptor for CD70/CD27L. (
  • Cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) is a co-inhibitory receptor that controls T cell activation during initiation and maintenance of adaptive immune responses. (
  • CD antigens appear to carry out cytokine receptor-like functions such as CD27, CD30 and CD40. (
  • For example, disclosed herein are genetically-modified cells comprising a chimeric antigen receptor or an inducible regulatory construct incorporating the co-stimulatory domains disclosed herein. (
  • Stimulation by antigen through the B cell receptor (BCR) followed by cognate T cell help drives proliferation and differentiation of antigen-specific naı̈ve B lymphocytes into memory B cells and plasma cells ( 1 , 2 ). (
  • Molecular cloning of human and mouse Fas cDNAs demonstrated that it belongs to the tumor necrosis factor receptor (TNFR) superfamily, which includes the low-affinity nerve growth factor receptor (NGFR), TNFR types I and II, human B-cell antigen CD40, T-cell antigen CD27 , and OX40 (Johnson et al. (
  • High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. (
  • TNFRSF receptors are expressed by a wide variety of immune cells including T cells and antigen-presenting cell (APC) populations, such as dendritic cells and macrophages, as well as by tumor cells themselves ( 2 , 3 ). (
  • Yun LW, Decarlo AA, Hunter N: Blockade of protease-activated receptors on T cells correlates with altered proteolysis of CD27 by gingipains of Porphyromonas gingivalis. (
  • Classically, robust CD4 + and CD8 + antigen-specific T-cell responses are dependent upon multiple inputs derived from various kinds of receptors on the T-cell surface ( 2 ⇓ ⇓ - 5 ). (
  • CD antigens participate in immune reaction as receptors for cell communication (e.g. adherence molecules, antigen recognizing receptors). (
  • CD antigens have been shown to be identical with receptors of cytokines such as CD25 (TAC antigen). (
  • The CD Antigen's designation isn't related to the biological function, thus CD antigens include receptors, glycans, adhesion molecules, membrane-bound enzymes, etc. (
  • It is a surface antigen on activated, but not on resting, T and B lymphocytes. (
  • Present on most peripheral blood T lymphocytes and medullary thymocytes, the CD27 antigen is upregulated upon activation with the release of a soluble form, 28 to 32 kDa. (
  • 4 ) on p. 1331 of this issue identify a subset of B lymphocytes in human infants that undergoes repertoire diversification via antigen-independent V gene SHM. (
  • CD27 antigen is found on medullary thymocytes, peripheral T lymphocytes, activated B lymphocytes and NK cells. (
  • Kawakami Y, Eliyahu S, Delgado CH, Robbins PF, Sakaguchi K, Appella E, Yannelli JR, Adema GJ, Miki T, Rosenberg SA (1994) Identification of a human melanoma antigen recognized by tumor-infiltrating lymphocytes associated with in vivo tumor rejection. (
  • During the course of development from precursor cells into functionally mature forms, lymphocytes display a complex pattern of surface antigens, some of which are acquired at certain stages while others are lost. (
  • In Hematology the morphological criteria is for the description of specific developmental stages of lymphocytes unlike in CD antigens which the use of monoclonal antibodies allows the objective and precise analysis and standardized typing of mature and immature normal and malignant cells of all hematopoietic cell lineages. (
  • Decreased frequency and activated phenotype of blood CD27 IgDIgM B lymphocytes is a permanent abnormality in systemic lupus erythematosus patients. (
  • To investigate the role of CD27 in multiple myeloma(MM), bone marrow samples from 165 newly diagnosed MM were analysed by flow cytometry. (
  • The proportion of CD27, CD28, IL-17A, IFN-γ and TNF-α of Vδ2 T cells was detected by the flow cytometry. (
  • CD27 has a unique expression profile, and its negative or weak expression is highly suggestive for MM. The 8 color flow cytometry can be used to analyze the expression of multiple antigens , which can provide a reliable evidence for the diagnosis and differential diagnosis of MM disease . (
  • Among different MRD techniques, flow cytometry provides additional information about antigen expression on tumor cells, which could potentially contribute to stratify MRD-positive patients. (
  • Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-human CD27 (LT27) APC. (
  • Design and Methods Bone marrow derived mesenchymal stem cells from healthy donors were analyzed and isolated by flow cytometry using a large panel of antibodies against surface antigens including CD271, MSCA-1, and CD56. (
  • Antibodies are as followsckappa- FITC /clambda-PE/ CD38-ECD/CD45-PERCP/CD19-PC7/ CD27-APC/CD138-BV421/ CD56-BV510. (
  • Terminally differentiated plasma cells express relatively few surface antigens, and do not express common pan-B cell markers, such as CD19 and CD20. (
  • Complete remission (CR) rates as high as 90% have been reported in children and adults with relapsed and refractory ALL treated with CAR-modified T cells targeting the B-cell-specific antigen CD19. (
  • Analysis of additional surface antigens like CD45, CD19, CD56, CD27, and the intracellular immunoglobulin light chain distribution were used to differentiate polyclonal from clonal PC. (
  • Expression of CD27, CD38, CD19, and IRF4 in unstimulated and stimulated thymic B cells. (
  • CD27 binds to CD70 and plays an important role in costimulation of T cell activation, and regulation of B cell differentiation and proliferation. (
  • 1989) CD25 mAb: epitopes recognised, effect on lymphocyte activation, mediation of ADCC in Leucocyte Typing IV: White Cell Differentiation Antigens Eds Knapp, W. et al. (
  • We find that B cells acquire CD27 at the centroblast stage and lose it progressively upon further differentiation. (
  • In particular, CD27 was recently found at low levels on all GC B cells from human tonsils, and dramatically up-regulated upon their in vitro differentiation into plasma cells ( 17 ). (
  • Whether CD27/CD70 interactions enhance Ig production by delivering differentiation signals to B cells or by sustaining expansion of differentiating cells is unclear. (
  • [6] Differentiation through a T cell-independent antigen stimulation (stimulation of a B cell that does not require the involvement of a T cell) can happen anywhere in the body [2] and results in short-lived cells that secrete IgM antibodies. (
  • Since B cell maturation also involves somatic hypermutation (a process completed before differentiation into a plasma cell), these antibodies frequently have a very high affinity for their antigen. (
  • Antigen-driven proliferation and differentiation of memory B cell to short-lived plasma cells induces high levels of protective antibodies ( 9 ). (
  • We therefore searched for alternative mechanisms that might ensure sustained proliferation and differentiation of memory B cells, independently of persisting antigen. (
  • CD27 (TNFRSF7) is a member of the TNFR family. (
  • CD27 (TNFRSF7), found on T cells and some natural killer (NK) cell populations, is expressed at particularly high levels by naïve T cells and regulatory T (Treg) cells. (
  • Expression of CD27, CD28 and IL-17A in peripheral blood from patients with colorectal carcinoma. (
  • To compare the different expressions of CD27, CD28, IL-17A, IFN-γ and TNF-α in the peripheral blood sampled from patients with colorectal carcinoma and healthy volunteers. (
  • In the CRC patients, the proportions of IL-17A of CD27- Vδ2 T cells and CD28+ Vδ2 T cells were higher than those of CD27+ Vδ2 T cells and CD28- Vδ2 T cells, whereas the expression of IFN-γ and TNF-α of CD27-Vδ2 T cells was lower than that of CD27+ Vδ2 T cells. (
  • We generated CD27-/- mice and found that CD27 makes essential contributions to mature CD4+ and CD8+ T cell function: CD27 supported antigen-specific expansion (but not effector cell maturation) of naïve T cells, independent of the cell cycle-promoting activities of CD28 and interleukin 2. (
  • Adoptive transfer of T and B cells into CD27/CD28 −/− mice revealed that CD27 promotes GC formation and consequent IgG production by two distinct mechanisms. (
  • Stimulation of CD27 on B cells by CD28 + Th cells accelerates GC formation, most likely by promoting centroblast expansion. (
  • In addition, CD27 on T cells can partially substitute for CD28 in supporting GC formation. (
  • Although IL-2 aAPC induced the greatest overall TIL expansion, IL-21 aAPC induced superior expansion of CD8 + T cells with a CD27 + CD28 + "young" phenotype and superior functional cytotoxic effector characteristics, without collateral expansion of Tregs. (
  • Indeed, it was shown that the use of minimally cultured "young" less-differentiated TIL, with longer telomeres and higher levels of the co-stimulatory molecules CD27 and CD28, is an important factor for success. (
  • CD antigens are involved in modulating the biological activities of cytokines such as CD4, CD28 and CD40. (
  • These cells are characterized by the surface pattern of effector-type or effector-memory T cells (expression of CD45RA, CD11a, CD11b, CLA, [alpha]E[beta]7 and absence of CD27 , CD28, CD56, CD62L). (
  • Immobilized recombinant human CD27 at 2 µg/ml binds recombinant human CD27L with an ED 50 = 8 - 40 ng/ml. (
  • thus, it has been speculated that the CD27L binds to a trimeric structure composed by three dimeric CD27 molecules. (
  • Immobilized human recombinant CD27 at 2 µg/ml binds human CD27L (BL cat. (
  • Therefore, we have developed HERA-CD27L, a novel he xavalent TNF r eceptor a gonist (HERA) targeting CD27 and mimicking the natural signaling concept. (
  • The hexavalent agonist significantly boosted antigen-specific T cell responses while having no effect on non-specific T cells and was superior over stabilized recombinant trivalent CD27L. (
  • In this manuscript, we describe the development of HERA-CD27L, a true CD27 agonist with a clearly defined forward-signaling mechanism of action. (
  • CD27 binds to CD70 and, through this interaction, plays an important role in T cell-B cell interaction. (
  • CD27 binds to CD70. (
  • The CD27 antigen cytoplasmic tail binds to the N-terminus of this protein. (
  • B cells within the germinal center then perish through spontaneous apoptosis unless their surface immunoglobulin binds with high affinity to antigen (present within immune complexes) on follicular dendritic cells. (
  • A transmembrane protein belonging to the tumor necrosis factor superfamily that specifically binds to CD27 ANTIGEN. (
  • Cytokine that binds to CD27. (
  • A modulating disulfide-linked T cell activation antigen. (
  • Plasma cells, also called plasma B cells, are white blood cells that originate in the bone marrow and secrete large quantities of proteins called antibodies in response to being presented specific substances called antigens. (
  • These antibodies are transported from the plasma cells by the blood plasma and the lymphatic system to the site of the target antigen (foreign substance), where they initiate its neutralization or destruction. (
  • This process favors, by selection for the ability to bind antigen with higher affinity, the activation and growth of B cell clones able to secrete antibodies of higher affinity for the antigen. (
  • To date, the objective of most clinical-use vaccines has been the generation of high titers of antigen-specific neutralizing antibodies. (
  • Recombinant antibodies were tested towards different antigens to determine the frequency of autoreactive and polyreactive clones. (
  • These surface antigens were identified initially by monoclonal antibodies and the designations of the antibodies were used often as synonyms for the cell surface proteins they detected, giving rise to a plethora of different names. (
  • CD antigen nomenclature describes different monoclonal antibodies from different sources that recognize identical antigens. (
  • In the last decade the wide palette of monoclonal antibodies has been prepared which recognise of CD antigens on human cells. (
  • Recently, we described a panel of monoclonal antibodies with superior selectivity for mesenchymal stem cells, including the monoclonal antibodies W8B2 against human mesenchymal stem cell antigen-1 (MSCA-1) and 39D5 against a CD56 epitope, which is not expressed on natural killer cells. (
  • Furthermore, plasma cells secreting antibodies to recall antigens are produced in vivo at levels proportional to the frequency of specific memory B cells, even several years after antigenic stimulation. (
  • Fully human antibodies against a specific antigen can be prepared by administering the antigen to a transgenic animal which has been modified to produce such antibodies in response to antigenic challenge, but whose endogenous loci have been disabled. (
  • The antigen-binding repertoire of the surface immunoglobulin is already unique for each clone, as a result of selective usage in each cell of different variable (V), diversity (D), and joining (J) region sequences, accompanied by the generation of random-linking N sequences (via the action of terminal transferase). (
  • Memory B cells and CD27. (
  • In the current study, we used a murine model of inducible self-antigen expression in the epidermis to elucidate the functional role of CD27 on autoreactive Teff cells. (
  • Expression of CD27 on Ag-specific Teff cells resulted in enhanced skin inflammation when compared with CD27-deficient Teff cells. (
  • CD27 signaling promoted the accumulation of IFN-γ and IL-2-producing T cells in skin draining lymph nodes in a cell-intrinsic fashion. (
  • Instead, signaling through CD27 resulted in the progressive survival of Teff cells during the autoimmune response. (
  • Using BH3 profiling to assess mitochondrial cell priming, we found that CD27-deficient cells were equally as sensitive as CD27-sufficient cells to mitochondrial outer membrane polarization upon exposure to either BH3 activator or sensitizer peptides. (
  • In contrast, CD27-deficient Teff cells expressed higher levels of active caspase 8. (
  • T cells expressing CD27 and CD70 play a key role in regulating B-cell activation and immunoglobulin synthesis. (
  • CD27 is recognized as a marker for memory B cells and is considered of diagnostic value in patients who have decreased numbers of switched memory B cells. (
  • Human CD27, amino acids (Thr21-Ile192) (Accession # NP_001233) was expressed in 293E cells. (
  • CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. (
  • CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. (
  • It has been reported that triggering CD27 plays an important role in the maturation of CD4+ and CD8+ effector cells. (
  • CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. (
  • Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. (
  • We here reveal the mechanism by which costimulatory CD27-CD70 interactions sustain survival of CD8 + effector T cells in infected tissue. (
  • By unbiased genome-wide gene expression analysis, we identified the Il2 gene as the most prominent CD27 target gene in murine CD8 + T cells. (
  • In vitro, CD27 directed IL-2 expression and promoted clonal expansion of primed CD8 + T cells exclusively by IL-2-dependent survival signaling. (
  • In mice intranasally infected with influenza virus, Cd27 -/- CD8 + effector T cells displayed reduced IL-2 production, accompanied by impaired accumulation in lymphoid organs and in the lungs, which constitute the tissue effector site. (
  • Reconstitution of Cd27 -/- CD8 + T cells with the IL2 gene restored their accumulation to wild-type levels in the lungs, but it did not rescue their accumulation in lymphoid organs. (
  • CD27 is expressed by a subset of thymocytes and virtually all mature T cells and is upregulated upon T-cell stimulation. (
  • We analyzed the frequencies of known B cell subsets in blood and observed a consistent reduction in the proportion of CD27 − IgD − B cells expressing all Ig isotypes in the blood in IBD (independent of severity of disease and treatment) compared to healthy controls. (
  • By mass cytometry we demonstrated that CD27 − IgD − B cells were proportionately enriched in the gut-associated lymphoid tissue (GALT) in IBD. (
  • Since production of TNFα is a feature of IBD relevant to therapies, we sought to determine whether B cells in GALT or the CD27 − IgD − subset in particular could contribute to pathology by secretion of TNFα or IL-10. (
  • We found that donor matched GALT and blood B cells are capable of producing TNFα as well as IL-10, but we saw no evidence that CD27 − IgD − B cells from blood expressed more TNFα compared to other subsets. (
  • The reduced proportion of CD27 − IgD − B cells in blood and the increased proportion in the gut implies that CD27 − IgD − B cells are recruited from the blood to the gut in IBD. (
  • CD27 − IgD − B cells have been implicated in immune responses to intestinal bacteria and recruitment to GALT, and may contribute to the intestinal inflammatory milieu in IBD. (
  • Upon CD27 binding, induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells. (
  • CD27 is expressed on subsets of B, T, and NK cells. (
  • By contrast, adaptive immune cells display immunologic memory that has 2 basic characteristics, antigen specificity and an amplified response upon subsequent exposure. (
  • Thus, these experiments identify an ability of innate immune cells to retain an intrinsic memory of prior activation, a function until now attributed only to antigen-specific adaptive immune cells. (
  • Studies on human B cells have featured CD27 as a marker and mediator of the B cell response. (
  • We have studied CD27 expression and function on B cells in the mouse. (
  • Expression of TNFR family member CD27 is restricted to naive and activated CD4 + and CD8 + T cells and subsets of B and NK cells. (
  • In vitro studies have established that CD27 promotes expansion of newly activated T cells ( 1 , 5 , 6 ). (
  • In CD27 −/− mice, generation and maintenance of CD4 + and CD8 + effector T cells in response to antigenic challenge is impaired ( 7 ). (
  • We have recently resolved that CD27 rescues activated T cells from death, and in this way, increments the yield of live T cells upon their clonal expansion at the site of priming. (
  • In addition, CD27 exerts a prosurvival effect on CD4 + and CD8 + T cells at tissue sites ( 8 ). (
  • CD27 + B cells are predominantly found in germinal centers (GCs) 3 and marginal zones ( 11 , 12 ). (
  • and 4) cord blood B cells lack CD27, while the percentage of CD27 + B cells in blood increases with age ( 16 ). (
  • Although these findings are in line with CD27 being a hallmark of primed B cells, they do not classify all CD27 + B cells as memory B cells. (
  • In this study, we have analyzed in detail the expression of CD27 on B cells in the mouse and find it in line with a contribution of CD27 to centroblast expansion. (
  • In vitro studies on human B cells indicate that CD27 can promote IgM, IgG, IgA, and IgE secretion ( 10 , 16 , 18 , 19 ). (
  • 20 ) found that CD27 did not drive expansion of activated B cells, but promoted the generation of a plasma cell phenotype and IgG secretion. (
  • However, other data argue that CD27 does promote expansion of activated B cells ( 19 , 21 ). (
  • Epstein-Barr virus (EBV) is associated with several cancers in which the tumor cells express EBV antigens EBNA1 and LMP2. (
  • Severe combined immunodeficient (SCID) mice inoculated with human CD27-expressing lymphoma cells were administered 1F5 to investigate direct antitumor effects. (
  • In humans, CD27 is expressed on the surface of the majority of T cells, memory B cells and plasma cells, and some natural killer (NK) cells ( 4-6 ). (
  • Recent studies suggested the existence of a subset of B cells that undergoes SHM in an antigen-independent fashion outside GCs ( 3 ), but this viewpoint has remained controversial. (
  • Engineering artificial antigen-presenting cells to express a diverse array of co-stimulatory molecules. (
  • To facilitate the therapeutic application of antigen-presenting cells (APCs), we have developed a cell-based artificial APC (aAPC) system by engineering K562 cells with lentiviruses to direct the stable expression and secretion of a variety of co-stimulatory molecules and cytokines. (
  • Importantly, B cells can present cognate tumour-derived antigens to T cells, with the functional consequences of such interactions being shaped by the B cell phenotype. (
  • naive B cells are CD27-, memory B-cells are CD27+ and plasma cells are CD27++. (
  • This antigen is expressed at high levels on normal human plasma cells. (
  • Pieces of the antigen (which are now known as antigenic peptides) are loaded onto MHC II molecules, and presented on its extracellular surface to CD4+ T cells (sometimes called T helper cells). (
  • First, the B cells must encounter a foreign antigen and are then required to be activated by T helper cells before they differentiate into specific cells. (
  • They divide rapidly and are still capable of internalizing antigens and presenting them to T cells. (
  • B cells then enter the germinal centers of secondary lymphoid follicles and undergo a process of random hypermutation in theirV immunoglobulin region genes, under the influence of activation-induced cytidine deaminase (AID), resulting in yet more diversity in the antigen-binding repertoire of their surface immunoglobulin. (
  • (B) Histograms show that both types of antigen-experienced T4 cells expressed significantly higher levels of surface PrP C than naïve T4 cells. (
  • Protein antigen (Ag)-based immunotherapies have the advantage to induce T cells with a potentially broad repertoire of specificities. (
  • Protein Ag-loaded DC were used as antigen presenting cells to stimulate T cells in vitro and subsequently analyzed in vivo for their anti-tumor effect via adoptive transfer, a treatment strategy widely studied in clinical trials as a therapy against various malignancies. (
  • Petersen TR, Dickgreber N, Hermans IF (2010) Tumor antigen presentation by dendritic cells. (
  • Blachere NE, Morris HK, Braun D, Saklani H, Di Santo JP, Darnell RB, Albert ML (2006) IL-2 is required for the activation of memory CD8 + T cells via antigen cross-presentation. (
  • An elusive goal of cellular immune vaccines is the generation of large numbers of antigen-specific T cells in response to subunit immunization. (
  • As such, identifying the factors that dictate the magnitude of antigen-specific T cells in response to immunization is of paramount importance. (
  • Others have described CD27 − IFN-γ + CD4 + T-cells as possibly predictive markers of TB disease. (
  • Physiological T cell help for B cells takes place in germinal centers (GC) in peripheral lymphoid organs, where follicular T helper (Tfh) cells interact with mature, antigen-stimulated B cells. (
  • Cells derived from aged cancer patients have a skewed immune repertoire toward cells that underwent extensive clonal expansion against persistent antigens, resulting in few tumor-specific CTLs ( 6 - 8 ). (
  • The target gene of scFv (47G4)-CD27-CD3ζ packaged in lentiviral particles could be used for high efficency tranduction of T cells and stably integrated expression. (
  • Methods Single CD27-IgD+ B cells were sorted by FACS from peripheral blood of SS patients and healthy donors (HD). (
  • The most commonly know CD antigens are CD4 and CD8 which are markers for T-helper and T-suppressor cells, respectively. (
  • The present disclosure provides novel co-stimulatory domains useful in genetically-modified cells to promote cell proliferation and/or promote cytokine secretion after antigen recognition. (
  • CD27 expression in IRF4 + cells. (
  • Memory B cells carrying somatically mutated immunoglobulin (Ig) genes survive in secondary lymphoid organs in the absence of antigen ( 3 ) and mediate secondary immune responses upon rechallenge. (
  • Naı̈ve B cells and two memory B cell subsets, carrying IgM (IgM memory B cells) or other isotypes due to class switching (switch memory B cells), were isolated from peripheral blood by sorting for expression of CD27 ( 13 ) and Ig isotypes ( Fig. 1 A), labeled with carboxyfluorescein diacetate succinimidyl ester (CFSE), and tested for their capacity to proliferate in response to CpG ( Fig. 1 B) ( 14 ). (
  • Moreover, such studies revealed a selectively enhancing influence of sleep on cytokines promoting the interaction between antigen presenting cells and T helper cells, like interleukin-12. (
  • CD27 Promotes CD4 Effector T Cell Survival in Response to Tissue Self-Antigen. (
  • Competition experiments showed that the IL-2 produced under the control of CD27 supported effector CD8 + T cell survival in the lungs in an autocrine manner. (
  • The recombinant human CD27-Fc fusion is expressed as a 400 amino acid protein consisting of Ala20 - Arg191 region of CD27 (UniProt accession #P26842) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions. (
  • Although extensively cultured CTLs retain antigen specificity for the tumor ( 10 ), they present striking alterations in function and gene and protein expressions ( 8 ), e.g. they are in an irreversible cell cycle arrest, resistant to apoptosis, with short telomeres and unable to respond to antigenic cues or IL-2 stimulation. (
  • The recommended ELISA Kit will likely detect the antigen in question with higher specificity in approved samples than the available alternatives. (
  • Recombinant protein corresponding to full-length human CD27. (
  • CD27 (NP_001233.1, 1 a.a. ~ 260 a.a) full-length human protein. (
  • 2007) Cross-reactivity of mAbs to human CD antigens with sheep leukocytes. (
  • CD27 is a 55-kDa disulfide-linked dimer that is a member of the nerve growth factor (NGF) super family. (
  • The CD27 antigen is a transmembrane glycoprotein disulfide-linked homodimer composed of 55 kDa monomers. (
  • In this study, we show that the Egr2-driven cell surface proteins LAG-3 and 4-1BB can identify dysfunctional tumor antigen-specific CD8 + TIL. (
  • The surface antigen CD138 (syndecan-1) is expressed at high levels. (
  • Another important surface antigen is CD319 (SLAMF7). (
  • Cell surface antigens of leukocytes are called CD antigens, and important for immune reactions of organisms. (
  • CD antigens have been characterized as both transmembrane proteins and cell surface proteins anchored to the plasma membrane via covalent attachment to fatty acid-containing glycolipids such as glycosylphosphatidylinositol (GPI). (
  • There were found non-significant decrease of CD27 and increase of CD56 expression on PCs in transformed group. (
  • Lower expression of CD56, CD27 and overexpression of CD44 with nestin was characteristic for both PCLs when compared to MM. (
  • Taken together, these results suggest that CD27 does not promote Teff cell survival by increasing expression of antiapoptotic BCL2 family members but instead acts by preferentially suppressing the cell-extrinsic apoptosis pathway, highlighting a previously unidentified role for CD27 in augmenting autoreactive Teff cell responses. (
  • To explore the expression of CD27 antigen in patients with multiple myeloma (MM), and its clinical diagnostic value, as well as the correlation of CD27 with clinical features and genetic abnormalities . (
  • Kara IO, Sahin B, Gunesacar R: Expression of soluble CD27 and interleukins-8 and -10 in B-cell chronic lymphocytic leukemia: correlation with disease stage and prognosis. (
  • van Oers MH, Pals ST, Evers LM, van der Schoot CE, Koopman G, Bonfrer JM, Hintzen RQ, von dem Borne AE, van Lier RA: Expression and release of CD27 in human B-cell malignancies. (
  • The expression of CD antigens is influenced by cytokines, such as binding of ligands to CD antigens which has shown to modulate the expression of cytokines. (
  • Lymphocyte subset infiltration patterns and HLA antigen status in colorectal carcinomas and adenomas. (
  • Cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) blockade can promote antitumor T cell immunity and clinical responses. (
  • Averaged variable importance projection to assess single biomarkers that maximally contribute to discriminate among outcome groups identified soluble CD27, CD14, and CD163 as the 3 most important with TNFα and LPS also highly relevant in providing separation. (
  • From an operational point of view, LTBI may best be defined as a state of persistent immune response to M. tuberculosis antigens detected either by the tuberculin skin test (TST) or by interferon (IFN)-γ release assay (IGRA) without evidence of clinically manifest TB. (
  • CD27 contributes to the early systemic immune response to mycobacterium tuberculosis infection but does not affect outcome. (
  • However, continued exposure to antigen through those low levels of immunoglobulin is important, as it partly determines the cell's lifespan. (
  • They concluded that antigen-specific immunoglobulin generation is significantly impaired following corticosteroid treatment. (
  • Your search returned 2 CD27 ELISA ELISA Kit across 1 supplier. (
  • The recommended ELISA Kit will likely detect the antigen better in the approved sample types than the available alternatives. (
  • Agonistic stimulation of CD27 is therefore a promising therapeutic concept in immuno-oncology intended to boost and sustain T cell driven anti-tumor responses. (
  • All in vitro studies rely on deliberate stimulation of CD27 with CD70 transfectants. (
  • Signaling through CD27 plays a role in T cell activation and memory. (
  • Soluble CD27 is an important prognostic marker of acute and chronic B cell maligcies. (
  • T cell priming takes place in lymph nodes and spleen, which receive the antigen by means of DC delivery. (
  • Primary CD4+ and CD8+ T cell responses to influenza virus were impaired in CD27-/- mice. (
  • Effects of deleting the gene encoding CD27 were most profound on T cell memory, reflected by delayed response kinetics and reduction of CD8+ virus-specific T cell numbers to the level seen in the primary response. (
  • The CD70-CD27 pathway plays an important role in the generation and maintenance of T cell immunity, in particular during antiviral responses. (
  • Find tissues and cell lines supported by DNA array analysis to express CD27 . (
  • New data in humans and mice now identify specific B cell populations that may have undergone antigen-independent hypermutation outside GCs. (
  • Collectively, these results suggest that the events associated with B cell development in humans and mice may not be far removed from those in other species, such as sheep, in which antigen-independent diversification occurs in the gut-associated lymphoid tissue (GALT). (
  • Unfortunately, T-cell responses to subunit immunization typically require multiple boosts to achieve even detectable antigen-specific T-cell numbers, which often have little clinical impact. (
  • T-cell responses to TB latency antigens, including heparin-binding haemagglutinin and DosR-regulon-encoded antigens have also been correlated with protection. (
  • CD antigens are found on practically all known cell types. (
  • In some cases CD antigens are expressed only at certain stages of development or under certain conditions, for example after cell activation or in certain disease conditions. (
  • Other more important CD antigens include the leukocytes integrins (CD11/CD18) and the hematopoietic stem cell marker CD34. (
  • In mouse, CD27 has been found on nearly all thymocytes excluding a population of CD46-CD8- precursors. (
  • The ELISA (enzyme-linked immunosorbent assay) is a widely used application for detecting and quantifying proteins and antigens from various samples. (
  • Also, other proteins which involved in the same pathway with Cd27 were listed below. (
  • Accordingly, GC formation in response to influenza virus infection was delayed in CD27 knockout mice. (