Substances that are recognized by the immune system and induce an immune reaction.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
Differentiation antigens found on thymocytes and on cytotoxic and suppressor T-lymphocytes. CD8 antigens are members of the immunoglobulin supergene family and are associative recognition elements in MHC (Major Histocompatibility Complex) Class I-restricted interactions.
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
Substances elaborated by bacteria that have antigenic activity.
A bifunctional enzyme that catalyzes the synthesis and HYDROLYSIS of CYCLIC ADP-RIBOSE (cADPR) from NAD+ to ADP-RIBOSE. It is a cell surface molecule which is predominantly expressed on LYMPHOID CELLS and MYELOID CELLS.
Glycoproteins found on immature hematopoietic cells and endothelial cells. They are the only molecules to date whose expression within the blood system is restricted to a small number of progenitor cells in the bone marrow.
Differentiation antigens expressed on B-lymphocytes and B-cell precursors. They are involved in regulation of B-cell proliferation.
A member of the tumor necrosis factor receptor superfamily with specificity for CD40 LIGAND. It is found on mature B-LYMPHOCYTES and some EPITHELIAL CELLS, lymphoid DENDRITIC CELLS. Evidence suggests that CD40-dependent activation of B-cells is important for generation of memory B-cells within the germinal centers. Mutations of the gene for CD40 antigen result in HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 3. Signaling of the receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
A membrane glycoprotein and differentiation antigen expressed on the surface of T-cells that binds to CD40 ANTIGENS on B-LYMPHOCYTES and induces their proliferation. Mutation of the gene for CD40 ligand is a cause of HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 1.
Unglycosylated phosphoproteins expressed only on B-cells. They are regulators of transmembrane Ca2+ conductance and thought to play a role in B-cell activation and proliferation.
Substances elaborated by viruses that have antigenic activity.
Costimulatory T-LYMPHOCYTE receptors that have specificity for CD80 ANTIGEN and CD86 ANTIGEN. Activation of this receptor results in increased T-cell proliferation, cytokine production and promotion of T-cell survival.
Acidic sulfated integral membrane glycoproteins expressed in several alternatively spliced and variable glycosylated forms on a wide variety of cell types including mature T-cells, B-cells, medullary thymocytes, granulocytes, macrophages, erythrocytes, and fibroblasts. CD44 antigens are the principle cell surface receptors for hyaluronate and this interaction mediates binding of lymphocytes to high endothelial venules. (From Abbas et al., Cellular and Molecular Immunology, 2d ed, p156)
Differentiation antigens expressed on pluripotential hematopoietic cells, most human thymocytes, and a major subset of peripheral blood T-lymphocytes. They have been implicated in integrin-mediated cellular adhesion and as signalling receptors on T-cells.
Glycolipid-anchored membrane glycoproteins expressed on cells of the myelomonocyte lineage including monocytes, macrophages, and some granulocytes. They function as receptors for the complex of lipopolysaccharide (LPS) and LPS-binding protein.
Glycoprotein members of the immunoglobulin superfamily which participate in T-cell adhesion and activation. They are expressed on most peripheral T-lymphocytes, natural killer cells, and thymocytes, and function as co-receptors or accessory molecules in the T-cell receptor complex.
Ratio of T-LYMPHOCYTES that express the CD4 ANTIGEN to those that express the CD8 ANTIGEN. This value is commonly assessed in the diagnosis and staging of diseases affecting the IMMUNE SYSTEM including HIV INFECTIONS.
Glycoproteins expressed on all mature T-cells, thymocytes, and a subset of mature B-cells. Antibodies specific for CD5 can enhance T-cell receptor-mediated T-cell activation. The B-cell-specific molecule CD72 is a natural ligand for CD5. (From Abbas et al., Cellular and Molecular Immunology, 2d ed, p156)
Antigens expressed primarily on the membranes of living cells during sequential stages of maturation and differentiation. As immunologic markers they have high organ and tissue specificity and are useful as probes in studies of normal cell development as well as neoplastic transformation.
A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.
Glycoproteins expressed on cortical thymocytes and on some dendritic cells and B-cells. Their structure is similar to that of MHC Class I and their function has been postulated as similar also. CD1 antigens are highly specific markers for human LANGERHANS CELLS.
Antibodies produced by a single clone of cells.
The 140 kDa isoform of NCAM (neural cell adhesion molecule) containing a transmembrane domain and short cytoplasmic tail. It is expressed by all lymphocytes mediating non-MHC restricted cytotoxicity and is present on some neural tissues and tumors.
Antigens expressed on the cell membrane of T-lymphocytes during differentiation, activation, and normal and neoplastic transformation. Their phenotypic characterization is important in differential diagnosis and studies of thymic ontogeny and T-cell function.
A membrane-bound or cytosolic enzyme that catalyzes the synthesis of CYCLIC ADP-RIBOSE (cADPR) from nicotinamide adenine dinucleotide (NAD). This enzyme generally catalyzes the hydrolysis of cADPR to ADP-RIBOSE, as well, and sometimes the synthesis of cyclic ADP-ribose 2' phosphate (2'-P-cADPR) from NADP.
Surface antigens expressed on myeloid cells of the granulocyte-monocyte-histiocyte series during differentiation. Analysis of their reactivity in normal and malignant myelomonocytic cells is useful in identifying and classifying human leukemias and lymphomas.
A costimulatory ligand expressed by ANTIGEN-PRESENTING CELLS that binds to CTLA-4 ANTIGEN with high specificity and to CD28 ANTIGEN with low specificity. The interaction of CD80 with CD28 ANTIGEN provides a costimulatory signal to T-LYMPHOCYTES, while its interaction with CTLA-4 ANTIGEN may play a role in inducing PERIPHERAL TOLERANCE.
Tetraspanin proteins found at high levels in cells of the lymphoid-myeloid lineage. CD53 antigens may be involved regulating the differentiation of T-LYMPHOCYTES and the activation of B-LYMPHOCYTES.
A cell adhesion protein that was originally identified as a heat stable antigen in mice. It is involved in METASTASIS and is highly expressed in many NEOPLASMS.
Zinc-binding metalloproteases that are members of the type II integral membrane metalloproteases. They are expressed by GRANULOCYTES; MONOCYTES; and their precursors as well as by various non-hematopoietic cells. They release an N-terminal amino acid from a peptide, amide or arylamide.
Any part or derivative of any protozoan that elicits immunity; malaria (Plasmodium) and trypanosome antigens are presently the most frequently encountered.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
A costimulatory ligand expressed by ANTIGEN-PRESENTING CELLS that binds to CD28 ANTIGEN with high specificity and to CTLA-4 ANTIGEN with low specificity. The interaction of CD86 with CD28 ANTIGEN provides a stimulatory signal to T-LYMPHOCYTES, while its interaction with CTLA-4 ANTIGEN may play a role in inducing PERIPHERAL TOLERANCE.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.
A tumor necrosis factor receptor subtype found in a variety of tissues and on activated LYMPHOCYTES. It has specificity for FAS LIGAND and plays a role in regulation of peripheral immune responses and APOPTOSIS. Multiple isoforms of the protein exist due to multiple ALTERNATIVE SPLICING. The activated receptor signals via a conserved death domain that associates with specific TNF RECEPTOR-ASSOCIATED FACTORS in the CYTOPLASM.
Antigens determined by leukocyte loci found on chromosome 6, the major histocompatibility loci in humans. They are polypeptides or glycoproteins found on most nucleated cells and platelets, determine tissue types for transplantation, and are associated with certain diseases.
Membrane antigens associated with maturation stages of B-lymphocytes, often expressed in tumors of B-cell origin.
High-molecular weight glycoproteins uniquely expressed on the surface of LEUKOCYTES and their hemopoietic progenitors. They contain a cytoplasmic protein tyrosine phosphatase activity which plays a role in intracellular signaling from the CELL SURFACE RECEPTORS. The CD45 antigens occur as multiple isoforms that result from alternative mRNA splicing and differential usage of three exons.
Process of classifying cells of the immune system based on structural and functional differences. The process is commonly used to analyze and sort T-lymphocytes into subsets based on CD antigens by the technique of flow cytometry.
Substances of fungal origin that have antigenic activity.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The major group of transplantation antigens in the mouse.
A 67-kDa sialic acid binding lectin that is specific for MYELOID CELLS and MONOCYTE-MACROPHAGE PRECURSOR CELLS. This protein is the smallest siglec subtype and contains a single immunoglobulin C2-set domain. It may play a role in intracellular signaling via its interaction with SHP-1 PROTEIN-TYROSINE PHOSPHATASE and SHP-2 PROTEIN-TYROSINE PHOSPHATASE.
Any part or derivative of a helminth that elicits an immune reaction. The most commonly seen helminth antigens are those of the schistosomes.
Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.
Cell-surface glycoprotein beta-chains that are non-covalently linked to specific alpha-chains of the CD11 family of leukocyte-adhesion molecules (RECEPTORS, LEUKOCYTE-ADHESION). A defect in the gene encoding CD18 causes LEUKOCYTE-ADHESION DEFICIENCY SYNDROME.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
A member of the tumor necrosis factor receptor superfamily that may play a role in the regulation of NF-KAPPA B and APOPTOSIS. They are found on activated T-LYMPHOCYTES; B-LYMPHOCYTES; NEUTROPHILS; EOSINOPHILS; MAST CELLS and NK CELLS. Overexpression of CD30 antigen in hematopoietic malignancies make the antigen clinically useful as a biological tumor marker. Signaling of the receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
Glycoproteins found on the membrane or surface of cells.
A critical subpopulation of regulatory T-lymphocytes involved in MHC Class I-restricted interactions. They include both cytotoxic T-lymphocytes (T-LYMPHOCYTES, CYTOTOXIC) and CD8+ suppressor T-lymphocytes.
Sites on an antigen that interact with specific antibodies.
A subtype of tetraspanin proteins that play a role in cell adhesion, cell motility, and tumor metastasis. CD9 antigens take part in the process of platelet activation and aggregation, the formation of paranodal junctions in neuronal tissue, and the fusion of sperm with egg.
A glycoprotein that is secreted into the luminal surface of the epithelia in the gastrointestinal tract. It is found in the feces and pancreaticobiliary secretions and is used to monitor the response to colon cancer treatment.
A subclass of HLA-D antigens that consist of alpha and beta chains. The inheritance of HLA-DR antigens differs from that of the HLA-DQ ANTIGENS and HLA-DP ANTIGENS.
A trisaccharide antigen expressed on glycolipids and many cell-surface glycoproteins. In the blood the antigen is found on the surface of NEUTROPHILS; EOSINOPHILS; and MONOCYTES. In addition, CD15 antigen is a stage-specific embryonic antigen.
Those proteins recognized by antibodies from serum of animals bearing tumors induced by viruses; these proteins are presumably coded for by the nucleic acids of the same viruses that caused the neoplastic transformation.
Established cell cultures that have the potential to propagate indefinitely.
A sialic acid-rich protein and an integral cell membrane mucin. It plays an important role in activation of T-LYMPHOCYTES.
Leukocyte differentiation antigens and major platelet membrane glycoproteins present on MONOCYTES; ENDOTHELIAL CELLS; PLATELETS; and mammary EPITHELIAL CELLS. They play major roles in CELL ADHESION; SIGNAL TRANSDUCTION; and regulation of angiogenesis. CD36 is a receptor for THROMBOSPONDINS and can act as a scavenger receptor that recognizes and transports oxidized LIPOPROTEINS and FATTY ACIDS.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A group of three different alpha chains (CD11a, CD11b, CD11c) that are associated with an invariant CD18 beta chain (ANTIGENS, CD18). The three resulting leukocyte-adhesion molecules (RECEPTORS, LEUKOCYTE ADHESION) are LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN-1; MACROPHAGE-1 ANTIGEN; and ANTIGEN, P150,95.
Large, transmembrane, non-covalently linked glycoproteins (alpha and beta). Both chains can be polymorphic although there is more structural variation in the beta chains. The class II antigens in humans are called HLA-D ANTIGENS and are coded by a gene on chromosome 6. In mice, two genes named IA and IE on chromosome 17 code for the H-2 antigens. The antigens are found on B-lymphocytes, macrophages, epidermal cells, and sperm and are thought to mediate the competence of and cellular cooperation in the immune response. The term IA antigens used to refer only to the proteins encoded by the IA genes in the mouse, but is now used as a generic term for any class II histocompatibility antigen.
A group of antigens that includes both the major and minor histocompatibility antigens. The former are genetically determined by the major histocompatibility complex. They determine tissue type for transplantation and cause allograft rejections. The latter are systems of allelic alloantigens that can cause weak transplant rejection.
Small glycoproteins found on both hematopoietic and non-hematopoietic cells. CD59 restricts the cytolytic activity of homologous complement by binding to C8 and C9 and blocking the assembly of the membrane attack complex. (From Barclay et al., The Leukocyte Antigen FactsBook, 1993, p234)
IMMUNOGLOBULINS on the surface of B-LYMPHOCYTES. Their MESSENGER RNA contains an EXON with a membrane spanning sequence, producing immunoglobulins in the form of type I transmembrane proteins as opposed to secreted immunoglobulins (ANTIBODIES) which do not contain the membrane spanning segment.
Nuclear antigen with a role in DNA synthesis, DNA repair, and cell cycle progression. PCNA is required for the coordinated synthesis of both leading and lagging strands at the replication fork during DNA replication. PCNA expression correlates with the proliferation activity of several malignant and non-malignant cell types.
Oligosaccharide antigenic determinants found principally on NK cells and T-cells. Their role in the immune response is poorly understood.
A transmembrane protein belonging to the tumor necrosis factor superfamily that specifically binds to CD27 ANTIGEN. It is found on activated T-LYMPHOCYTES; B-LYMPHOCYTES; and DENDRITIC CELLS where it plays a role in stimulating the proliferation of CD4-POSITIVE T-LYMPHOCYTES and CD8-POSITIVE T-LYMPHOCYTES.
A ubiquitously expressed complement receptor that binds COMPLEMENT C3B and COMPLEMENT C4B and serves as a cofactor for their inactivation. CD46 also interacts with a wide variety of pathogens and mediates immune response.
A class of animal lectins that bind to carbohydrate in a calcium-dependent manner. They share a common carbohydrate-binding domain that is structurally distinct from other classes of lectins.
Glycoproteins with a wide distribution on hematopoietic and non-hematopoietic cells and strongly expressed on macrophages. CD58 mediates cell adhesion by binding to CD2; (ANTIGENS, CD2); and this enhances antigen-specific T-cell activation.
55-kDa antigens found on HELPER-INDUCER T-LYMPHOCYTES and on a variety of other immune cell types. CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in MAJOR HISTOCOMPATIBILITY COMPLEX class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as INTERLEUKIN-15 receptors and bind to the HIV receptors, binding directly to the HIV ENVELOPE PROTEIN GP120.
A ubiquitously expressed membrane glycoprotein. It interacts with a variety of INTEGRINS and mediates responses to EXTRACELLULAR MATRIX PROTEINS.
A CD antigen that contains a conserved I domain which is involved in ligand binding. When combined with CD18 the two subunits form MACROPHAGE-1 ANTIGEN.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A glycoprotein that is a kallikrein-like serine proteinase and an esterase, produced by epithelial cells of both normal and malignant prostate tissue. It is an important marker for the diagnosis of prostate cancer.
An integrin alpha subunit of approximately 150-kDa molecular weight. It is expressed at high levels on monocytes and combines with CD18 ANTIGEN to form the cell surface receptor INTEGRIN ALPHAXBETA2. The subunit contains a conserved I-domain which is characteristic of several of alpha integrins.
The lipopolysaccharide-protein somatic antigens, usually from gram-negative bacteria, important in the serological classification of enteric bacilli. The O-specific chains determine the specificity of the O antigens of a given serotype. O antigens are the immunodominant part of the lipopolysaccharide molecule in the intact bacterial cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
A specific HLA-A surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-A*02 allele family.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Progenitor cells from which all blood cells derive.
The number of CD4-POSITIVE T-LYMPHOCYTES per unit volume of BLOOD. Determination requires the use of a fluorescence-activated flow cytometer.
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
Carbohydrate antigens expressed by malignant tissue. They are useful as tumor markers and are measured in the serum by means of a radioimmunoassay employing monoclonal antibodies.
GPI-linked membrane proteins broadly distributed among hematopoietic and non-hematopoietic cells. CD55 prevents the assembly of C3 CONVERTASE or accelerates the disassembly of preformed convertase, thus blocking the formation of the membrane attack complex.
Cell adhesion molecules present on virtually all monocytes, platelets, and granulocytes. CD31 is highly expressed on endothelial cells and concentrated at the junctions between them.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Membrane glycoproteins consisting of an alpha subunit and a BETA 2-MICROGLOBULIN beta subunit. In humans, highly polymorphic genes on CHROMOSOME 6 encode the alpha subunits of class I antigens and play an important role in determining the serological specificity of the surface antigen. Class I antigens are found on most nucleated cells and are generally detected by their reactivity with alloantisera. These antigens are recognized during GRAFT REJECTION and restrict cell-mediated lysis of virus-infected cells.
Tetraspanin proteins that are involved in a variety of cellular functions including BASEMENT MEMBRANE assembly, and in the formation of a molecular complexes on the surface of LYMPHOCYTES.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
A member of the tumor necrosis factor receptor superfamily that is specific for 4-1BB LIGAND. It is found in a variety of immune cell types including activated T-LYMPHOCYTES; NATURAL KILLER CELLS; and DENDRITIC CELLS. Activation of the receptor on T-LYMPHOCYTES plays a role in their expansion, production of cytokines and survival. Signaling by the activated receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Proteins prepared by recombinant DNA technology.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.
Polymorphic class I human histocompatibility (HLA) surface antigens present on almost all nucleated cells. At least 20 antigens have been identified which are encoded by the A locus of multiple alleles on chromosome 6. They serve as targets for T-cell cytolytic responses and are involved with acceptance or rejection of tissue/organ grafts.
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).
Receptors present on activated T-LYMPHOCYTES and B-LYMPHOCYTES that are specific for INTERLEUKIN-2 and play an important role in LYMPHOCYTE ACTIVATION. They are heterotrimeric proteins consisting of the INTERLEUKIN-2 RECEPTOR ALPHA SUBUNIT, the INTERLEUKIN-2 RECEPTOR BETA SUBUNIT, and the INTERLEUKIN RECEPTOR COMMON GAMMA-CHAIN.
Sets of cell surface antigens located on BLOOD CELLS. They are usually membrane GLYCOPROTEINS or GLYCOLIPIDS that are antigenically distinguished by their carbohydrate moieties.
Those hepatitis B antigens found on the surface of the Dane particle and on the 20 nm spherical and tubular particles. Several subspecificities of the surface antigen are known. These were formerly called the Australia antigen.
Ubiquitously-expressed tetraspanin proteins that are found in late ENDOSOMES and LYSOSOMES and have been implicated in intracellular transport of proteins.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Tetraspanin proteins found associated with LAMININ-binding INTEGRINS. The CD151 antigens may play a role in the regulation of CELL MOTILITY.
A component of the B-cell antigen receptor that is involved in B-cell antigen receptor heavy chain transport to the PLASMA MEMBRANE. It is expressed almost exclusively in B-LYMPHOCYTES and serves as a useful marker for B-cell NEOPLASMS.
An encapsulated lymphatic organ through which venous blood filters.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Human immune-response or Class II antigens found mainly, but not exclusively, on B-lymphocytes and produced from genes of the HLA-D locus. They are extremely polymorphic families of glycopeptides, each consisting of two chains, alpha and beta. This group of antigens includes the -DR, -DQ and -DP designations, of which HLA-DR is most studied; some of these glycoproteins are associated with certain diseases, possibly of immune etiology.
A membrane-bound tumor necrosis family member found primarily on activated T-LYMPHOCYTES that binds specifically to CD30 ANTIGEN. It may play a role in INFLAMMATION and immune regulation.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
A class of enzymes involved in the hydrolysis of the N-glycosidic bond of nitrogen-linked sugars.
A form of undifferentiated malignant LYMPHOMA usually found in central Africa, but also reported in other parts of the world. It is commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. B-cell antigens are expressed on the immature cells that make up the tumor in virtually all cases of Burkitt lymphoma. The Epstein-Barr virus (HERPESVIRUS 4, HUMAN) has been isolated from Burkitt lymphoma cases in Africa and it is implicated as the causative agent in these cases; however, most non-African cases are EBV-negative.
Molecules on the surface of B- and T-lymphocytes that recognize and combine with specific antigens.
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.
An alpha-integrin subunit found on lymphocytes, granulocytes, macrophages and monocytes. It combines with the integrin beta2 subunit (CD18 ANTIGEN) to form LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN-1.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Antigens of the virion of the HEPATITIS B VIRUS or the Dane particle, its surface (HEPATITIS B SURFACE ANTIGENS), core (HEPATITIS B CORE ANTIGENS), and other associated antigens, including the HEPATITIS B E ANTIGENS.
The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.
The processes triggered by interactions of ANTIBODIES with their ANTIGENS.
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
Mice homozygous for the mutant autosomal recessive gene "scid" which is located on the centromeric end of chromosome 16. These mice lack mature, functional lymphocytes and are thus highly susceptible to lethal opportunistic infections if not chronically treated with antibiotics. The lack of B- and T-cell immunity resembles severe combined immunodeficiency (SCID) syndrome in human infants. SCID mice are useful as animal models since they are receptive to implantation of a human immune system producing SCID-human (SCID-hu) hematochimeric mice.
Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
A heterogeneous group of immunocompetent cells that mediate the cellular immune response by processing and presenting antigens to the T-cells. Traditional antigen-presenting cells include MACROPHAGES; DENDRITIC CELLS; LANGERHANS CELLS; and B-LYMPHOCYTES. FOLLICULAR DENDRITIC CELLS are not traditional antigen-presenting cells, but because they hold antigen on their cell surface in the form of IMMUNE COMPLEXES for B-cell recognition they are considered so by some authors.
The type species of LYMPHOCRYPTOVIRUS, subfamily GAMMAHERPESVIRINAE, infecting B-cells in humans. It is thought to be the causative agent of INFECTIOUS MONONUCLEOSIS and is strongly associated with oral hairy leukoplakia (LEUKOPLAKIA, HAIRY;), BURKITT LYMPHOMA; and other malignancies.
T-cell receptors composed of CD3-associated alpha and beta polypeptide chains and expressed primarily in CD4+ or CD8+ T-cells. Unlike immunoglobulins, the alpha-beta T-cell receptors recognize antigens only when presented in association with major histocompatibility (MHC) molecules.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Class I human histocompatibility (HLA) surface antigens encoded by more than 30 detectable alleles on locus B of the HLA complex, the most polymorphic of all the HLA specificities. Several of these antigens (e.g., HLA-B27, -B7, -B8) are strongly associated with predisposition to rheumatoid and other autoimmune disorders. Like other class I HLA determinants, they are involved in the cellular immune reactivity of cytolytic T lymphocytes.
The altered state of immunologic responsiveness resulting from initial contact with antigen, which enables the individual to produce antibodies more rapidly and in greater quantity in response to secondary antigenic stimulus.
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
The phenomenon of target cell destruction by immunologically active effector cells. It may be brought about directly by sensitized T-lymphocytes or by lymphoid or myeloid "killer" cells, or it may be mediated by cytotoxic antibody, cytotoxic factor released by lymphoid cells, or complement.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
A melanosome-specific protein that plays a role in the expression, stability, trafficking, and processing of GP100 MELANOMA ANTIGEN, which is critical to the formation of Stage II MELANOSOMES. The protein is used as an antigen marker for MELANOMA cells.
A widely distributed cell surface transmembrane glycoprotein that stimulates the synthesis of MATRIX METALLOPROTEINASES. It is found at high levels on the surface of malignant NEOPLASMS and may play a role as a mediator of malignant cell behavior.
A general term for various neoplastic diseases of the lymphoid tissue.
An albumin obtained from the white of eggs. It is a member of the serpin superfamily.
Antigens associated with specific proteins of the human adult T-cell immunodeficiency virus (HIV); also called HTLV-III-associated and lymphadenopathy-associated virus (LAV) antigens.
An inhibitory T CELL receptor that is closely related to CD28 ANTIGEN. It has specificity for CD80 ANTIGEN and CD86 ANTIGEN and acts as a negative regulator of peripheral T cell function. CTLA-4 antigen is believed to play role in inducing PERIPHERAL TOLERANCE.
A promyelocytic cell line derived from a patient with ACUTE PROMYELOCYTIC LEUKEMIA. HL-60 cells lack specific markers for LYMPHOID CELLS but express surface receptors for FC FRAGMENTS and COMPLEMENT SYSTEM PROTEINS. They also exhibit phagocytic activity and responsiveness to chemotactic stimuli. (From Hay et al., American Type Culture Collection, 7th ed, pp127-8)
A widely expressed transmembrane glycoprotein that functions as a METASTASIS suppressor protein. It is underexpressed in a variety of human NEOPLASMS.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
A group of differentiation surface antigens, among the first to be discovered on thymocytes and T-lymphocytes. Originally identified in the mouse, they are also found in other species including humans, and are expressed on brain neurons and other cells.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
The specific failure of a normally responsive individual to make an immune response to a known antigen. It results from previous contact with the antigen by an immunologically immature individual (fetus or neonate) or by an adult exposed to extreme high-dose or low-dose antigen, or by exposure to radiation, antimetabolites, antilymphocytic serum, etc.
Manifestations of the immune response which are mediated by antigen-sensitized T-lymphocytes via lymphokines or direct cytotoxicity. This takes place in the absence of circulating antibody or where antibody plays a subordinate role.
A single, unpaired primary lymphoid organ situated in the MEDIASTINUM, extending superiorly into the neck to the lower edge of the THYROID GLAND and inferiorly to the fourth costal cartilage. It is necessary for normal development of immunologic function early in life. By puberty, it begins to involute and much of the tissue is replaced by fat.
Endogenous tissue constituents that have the ability to interact with AUTOANTIBODIES and cause an immune response.
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
Nuclear antigens encoded by VIRAL GENES found in HUMAN HERPESVIRUS 4. At least six nuclear antigens have been identified.
A soluble substance elaborated by antigen- or mitogen-stimulated T-LYMPHOCYTES which induces DNA synthesis in naive lymphocytes.
A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.
A cell line derived from cultured tumor cells.
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
A sex-specific cell surface antigen produced by the sex-determining gene of the Y chromosome in mammals. It causes syngeneic grafts from males to females to be rejected and interacts with somatic elements of the embryologic undifferentiated gonad to produce testicular organogenesis.
A cell adhesion molecule of the immunoglobulin superfamily that is expressed in ENDOTHELIAL CELLS and is involved in INTERCELLULAR JUNCTIONS.
Antigens stimulating the formation of, or combining with heterophile antibodies. They are cross-reacting antigens found in phylogenetically unrelated species.
CD4-positive T cells that inhibit immunopathology or autoimmune disease in vivo. They inhibit the immune response by influencing the activity of other cell types. Regulatory T-cells include naturally occurring CD4+CD25+ cells, IL-10 secreting Tr1 cells, and Th3 cells.
Antibodies obtained from a single clone of cells grown in mice or rats.
Antigenic determinants recognized and bound by the T-cell receptor. Epitopes recognized by the T-cell receptor are often located in the inner, unexposed side of the antigen, and become accessible to the T-cell receptors after proteolytic processing of the antigen.
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
A heterodimeric protein that is a cell surface antigen associated with lymphocyte activation. The initial characterization of this protein revealed one identifiable heavy chain (ANTIGENS, CD98 HEAVY CHAIN) and an indeterminate smaller light chain. It is now known that a variety of light chain subunits (ANTIGENS, CD98 LIGHT CHAINS) can dimerize with the heavy chain. Depending upon its light chain composition a diverse array of functions can be found for this protein. Functions include: type L amino acid transport, type y+L amino acid transport and regulation of cellular fusion.
The hepatitis B antigen within the core of the Dane particle, the infectious hepatitis virion.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
The complex formed by the binding of antigen and antibody molecules. The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES.
They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system.
The sum of the weight of all the atoms in a molecule.
Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.
A group of the D-related HLA antigens found to differ from the DR antigens in genetic locus and therefore inheritance. These antigens are polymorphic glycoproteins comprising alpha and beta chains and are found on lymphoid and other cells, often associated with certain diseases.
Immunoglobulins produced in response to VIRAL ANTIGENS.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
A glycolipid, cross-species antigen that induces production of antisheep hemolysin. It is present on the tissue cells of many species but absent in humans. It is found in many infectious agents.
Elements of limited time intervals, contributing to particular results or situations.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
An inhibitory B7 antigen that has specificity for the T-CELL receptor PROGRAMMED CELL DEATH 1 PROTEIN. CD274 antigen provides negative signals that control and inhibit T-cell responses and is found at higher than normal levels on tumor cells, suggesting its potential role in TUMOR IMMUNE EVASION.
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
A species of POLYOMAVIRUS originally isolated from Rhesus monkey kidney tissue. It produces malignancy in human and newborn hamster kidney cell cultures.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
Substances that augment, stimulate, activate, potentiate, or modulate the immune response at either the cellular or humoral level. The classical agents (Freund's adjuvant, BCG, Corynebacterium parvum, et al.) contain bacterial antigens. Some are endogenous (e.g., histamine, interferon, transfer factor, tuftsin, interleukin-1). Their mode of action is either non-specific, resulting in increased immune responsiveness to a wide variety of antigens, or antigen-specific, i.e., affecting a restricted type of immune response to a narrow group of antigens. The therapeutic efficacy of many biological response modifiers is related to their antigen-specific immunoadjuvanticity.
Antigens that exist in alternative (allelic) forms in a single species. When an isoantigen is encountered by species members who lack it, an immune response is induced. Typical isoantigens are the BLOOD GROUP ANTIGENS.
Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell.
A melanosome-associated protein that plays a role in the maturation of the MELANOSOME.
The genetic region which contains the loci of genes which determine the structure of the serologically defined (SD) and lymphocyte-defined (LD) TRANSPLANTATION ANTIGENS, genes which control the structure of the IMMUNE RESPONSE-ASSOCIATED ANTIGENS, HUMAN; the IMMUNE RESPONSE GENES which control the ability of an animal to respond immunologically to antigenic stimuli, and genes which determine the structure and/or level of the first four components of complement.
Bone marrow-derived lymphocytes that possess cytotoxic properties, classically directed against transformed and virus-infected cells. Unlike T CELLS; and B CELLS; NK CELLS are not antigen specific. The cytotoxicity of natural killer cells is determined by the collective signaling of an array of inhibitory and stimulatory CELL SURFACE RECEPTORS. A subset of T-LYMPHOCYTES referred to as NATURAL KILLER T CELLS shares some of the properties of this cell type.
A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
Form of passive immunization where previously sensitized immunologic agents (cells or serum) are transferred to non-immune recipients. When transfer of cells is used as a therapy for the treatment of neoplasms, it is called adoptive immunotherapy (IMMUNOTHERAPY, ADOPTIVE).

Expression of the cell adhesion molecules on leukocytes that demarginate during acute maximal exercise. (1/1480)

The pulmonary vascular bed is an important reservoir for the marginated pool of leukocytes that can be mobilized by exercise or catecholamines. This study was designed to determine the phenotypic characteristics of leukocytes that are mobilized into the circulation during exercise. Twenty healthy volunteers performed incremental exercise to exhaustion [maximal O2 consumption (VO2 max)] on a cycle ergometer. Blood was collected at baseline, at 3-min intervals during exercise, at VO2 max, and 30 min after exercise. Total white cell, polymorphonuclear leukocyte (PMN), and lymphocyte counts increased with exercise to VO2 max (P < 0.05). Flow cytometric analysis showed that the mean fluorescence intensity of L-selectin on PMN (from 14.9 +/- 1 at baseline to 9.5 +/- 1.6 at VO2 max, P < 0.05) and lymphocytes (from 11.7 +/- 1.2 at baseline to 8 +/- 0.8 at VO2 max, P < 0.05) decreased with exercise. Mean fluorescence intensity of CD11b on PMN increased with exercise (from 10.2 +/- 0.6 at baseline to 25 +/- 2.5 at VO2 max, P < 0.002) but remained unchanged on lymphocytes. Myeloperoxidase levels in PMN did not change with exercise. In vitro studies showed that neither catecholamines nor plasma collected at VO2 max during exercise changed leukocyte L-selectin or CD11b levels. We conclude that PMN released from the marginated pool during exercise express low levels of L-selectin and high levels of CD11b.  (+info)

Androgen influence on lacrimal gland apoptosis, necrosis, and lymphocytic infiltration. (2/1480)

PURPOSE: Previous studies have shown that ovariectomy and hypophysectomy cause regression of the lacrimal gland and have implicated androgens as trophic hormones that support the gland. The purposes of this study were to test the hypothesis that glandular regression after ovariectomy is due to apoptosis, to identify the cell type or types that undergo apoptosis, to survey the time course of the apoptosis, and to determine whether ovariectomy-induced apoptosis could be prevented by dihydrotestosterone (DHT) treatment. METHODS: Groups of sexually mature female New Zealand White rabbits were ovariectomized and killed at various time periods up to 9 days. Additional groups of ovariectomized rabbits were treated with 4 mg/kg DHT per day. At each time period, sham-operated rabbits were used as controls. Lacrimal glands were removed and processed for analysis of apoptosis as assessed by DNA fragmentation and for morphologic examination. DNA fragmentation was determined using the TdT-dUTP terminal nick-end labeling assay and by agarose gel electrophoresis. Labeled nuclei were quantified by automated densitometry. Sections were also stained for RTLA (rabbit thymic lymphocyte antigen), rabbit CD18, and La antigen. Morphology was evaluated by both light and electron microscopy. RESULTS: The time course of apoptosis exhibited two phases, a rapid and transient phase and a second prolonged phase. A transient phase peaked at approximately 4 to 6 hours after ovariectomy. The values for degraded DNA as a percentage of total nuclear area were 4.29%+/-0.79% and 4.26%+/-0.54%, respectively. The values for sham-operated controls examined at the same time periods were 1.77%+/-0.08% and 0.82%+/-0.21%, respectively. The percentage of degraded DNA at 24 hours after ovariectomy was not different from controls examined at the same interval after sham operation. The percentage of degraded DNA 6 days after ovariectomy was significantly increased (8.5%+/-2.4%), compared with sham-operated animals at the same time period (0.68%+/-0.03%). DNA laddering was more pronounced after ovariectomy. Dihydrotestosterone treatment in ovariectomized rabbits suppressed the increase in DNA degradation. Morphologic examination of lacrimal gland sections indicated that ovariectomy caused apoptosis of interstitial cells rather than acinar or ductal epithelial cells. Tissue taken 4 hours and 6 days after ovariectomy showed nuclear chromatin condensation principally in plasma cells. Increased numbers of macrophages were also evident. Significant levels of cell degeneration and cell debris, characteristic of necrosis, were observed in acinar regions 6 days after ovariectomy. Dihydrotestosterone prevented this necrosis. Increased numbers of RTLA+, CD18+, and La+ interstitial cells were also evident 6 days after ovariectomy. In addition, ovariectomy increased La expression in ductal cells. Dihydrotestosterone treatment prevented the increase in numbers of lymphoid cells and La expression. Dihydrotestosterone also promoted the appearance of mitotic figures in acinar cells and increased the sizes of acini by 43% (P < 0.05). CONCLUSIONS: Glandular atrophy observed after ovariectomy is likely to proceed by necrosis of acinar cells rather than apoptosis. This process begins with an apparent time lag after a rapid phase of interstitial cell apoptosis. These processes are accompanied by increased lymphocytic infiltration. These results suggest that a critical level of androgen is necessary to maintain lacrimal gland structure and function and that a decrease in available androgen below this level could trigger lacrimal gland apoptosis and necrosis, and an autoimmune response. Because apoptotic and necrotic cell fragments may be sources of autoantigens that can be processed and presented to initiate an autoimmune reaction, we surmise that cell death triggered by androgen withdrawal may trigger an autoimmune response such as that encountered in Sjogren's syndrome. (ABSTRACT TRUNCATED)  (+info)

Antibodies to CD18 influence neutrophil migration through extracellular matrix. (3/1480)

Mac-1 (CD11b/CD18) is known to be involved in neutrophil (PMN) adhesion to endothelial cells and extracellular matrix. Although antibodies to CD 18 are being tested for therapy in humans, their role in PMN migration through the extracellular matrix is unknown. We used direct visualization to quantify PMN motility through reconstituted, three-dimensional gels of collagen type I. Gels were prepared with different concentrations of collagen (ranging from 0.1 to 1.0 mg/mL) and PMN migration was examined in the presence and absence of antibodies to CD18 (anti-CD18), with and without stimulation by N-formyl peptides. In low-concentration gels (<0.6 mg/mL), anti-CD18 had a significant influence on PMN migration, increasing motility in unstimulated PMN by 90% at 0.3 mg/mL collagen, and decreasing motility in N-formyl-methionyl-leucyl-phenylalanine (fMLP)-stimulated PMN by 70% at 0.4 mg/mL collagen. But antiCD18 had no effect on the rate of cell migration through high-concentration collagen gels (>0.6 mg/mL). PMN migration through collagen gels is CD18-dependent but only under conditions of high hydration, suggesting that CD18-mediated effects (e.g., adhesion to gel fibers) are only important when the fiber density is relatively low. Anti-CD18 inhibited, but did not eliminate, the adhesion of fMLP-stimulated PMN to the surface of collagen gels, suggesting that cells use multiple mechanisms for gaining traction within the gel. Because of the multiple modes of interaction between motile cells and the deformable fiber matrix, blockade of one component, such as CD18, can enhance the rate of cell migration under one set of conditions, and inhibit under another.  (+info)

Characterization of beta2 (CD18) integrin phosphorylation in phorbol ester-activated T lymphocytes. (4/1480)

Integrins are transmembrane proteins involved in cell-cell and cell-extracellular-matrix interactions. The affinity and avidity of integrins for their ligands change in response to cytoplasmic signals. This 'inside-out' activation has been reported to occur also with beta2 integrins (CD18). The beta2 integrin subunit has previously been shown to become phosphorylated in T lymphocytes on cytoplasmic serine and the functionally important threonine residues after treatment with phorbol esters or on triggering of T-cell receptors. We have now characterized the phosphorylation of beta2 integrins in T-cells in more detail. When T-cells were activated by phorbol esters the phosphorylation was mainly on Ser756. After inhibition of serine/threonine phosphatases, phosphorylation was also found in two of the threonine residues in the threonine triplet 758-760 of the beta2 cytoplasmic domain. Activation of T-cells by phorbol esters resulted in phosphorylation in only approx. 10% of the integrin molecules. Okadaic acid increased this phosphorylation to approx. 30% of the beta2 molecules, assuming three phosphorylation sites. This indicates that a strong dynamic phosphorylation exists in serine and threonine residues of the beta2 integrins.  (+info)

Differential regulation of beta1 integrins by chemoattractants regulates neutrophil migration through fibrin. (5/1480)

Chemoattractants differ in their capacity to stimulate neutrophils to adhere to and to migrate through matrices containing fibrin. Formyl methionyl leucyl phenylalanine (fMLP) stimulates neutrophils to adhere closely to, but not to migrate into, fibrin gels. Leukotriene B4 (LTB4) stimulates neutrophils to adhere loosely to and to migrate through fibrin gels. We report that alpha5beta1 integrins regulate the different migratory behaviors on fibrin gels of neutrophils in response to these chemoattractants. fMLP, but not LTB4, activated neutrophil beta1 integrins, as measured by binding of mAb 15/7 to an activation epitope on the beta1 integrins. Antibodies or peptides that block alpha5beta1 integrins prevented fMLP-stimulated neutrophils from forming zones of close apposition on fibrin and reversed fMLP's inhibitory effect on neutrophil chemotaxis through fibrin. In contrast, neither peptides nor antibodies that block beta1 integrins affected the capacity of LTB4-stimulated neutrophils to form zones of loose apposition or to migrate through fibrin gels. These results suggest that chemoattractants generate at least two different messages that direct neutrophils, and perhaps other leukocytes, to accumulate at specific anatomic sites: a general message that induces neutrophils to crawl and a specific message that prepares neutrophils to stop when they contact appropriate matrix proteins for activated beta1 integrins.  (+info)

Monocyte activation in rheumatoid arthritis (RA): increased integrin, Fc gamma and complement receptor expression and the effect of glucocorticoids. (6/1480)

The aim of this work was to study the expression of beta 1- and beta 2-integrins, CR1, CD44 and Fc gamma receptors on peripheral blood monocytes in RA. The expression of these receptors was measured by flow cytometry, before and after treatment with low-dose prednisolone. Expression of the same receptors was also measured before and after treatment with metyrapone, a substance that inhibits the synthesis of cortisol in the adrenals. The expression of the beta 2-integrins CD11a, CD11b and CD18, of CD35 (CR1), and of Fc gamma RII and Fc gamma RI (CD32 and CD64) on monocytes was elevated in the RA patients compared with healthy controls, while the expression of the beta 1-integrins (CD29, CD49d, CD49f) was unaffected. A significant correlation between monocyte expression of CD64 and C-reactive protein (CRP), and blood platelet count, respectively, was found in the group of patients with RA. After 4-6 weeks of treatment with low-dose prednisolone, the expression on the monocytes of CD11a, CD11b, CD18, CD35, CD32 and CD64 was normalized. A significant correlation (r = 0.64, P = 0.02) was found between the decrease in expression of CD11b and clinical improvement after prednisolone treatment. Two days of metyrapone treatment, which significantly lowered the serum cortisol levels, elevated the expression of CD35 and CD49f. Priming of peripheral monocytes seems to be one of the mechanisms behind the recruitment of monocytes to the rheumatoid synovium. One reason for the good clinical effects of prednisolone in RA could be a down-regulation of adhesion and phagocytosis receptors on monocytes.  (+info)

Specific activation of leukocyte beta2 integrins lymphocyte function-associated antigen-1 and Mac-1 by chemokines mediated by distinct pathways via the alpha subunit cytoplasmic domains. (7/1480)

We show that CC chemokines induced a sustained increase in monocyte adhesion to intercellular adhesion molecule-1 that was mediated by Mac-1 (alphaMbeta2) but not lymphocyte function-associated antigen-1 (LFA-1; alphaLbeta2). In contrast, staining for an activation epitope revealed a rapid and transient up-regulation of LFA-1 activity by monocyte chemotactic protein-1 (MCP-1) in monocytes and Jurkat CCR2 chemokine receptor transfectants or by stromal-derived factor-1alpha in Jurkat cells. Differential kinetics for activation of Mac-1 (sustained) and LFA-1 (transient) avidity in response to stromal-derived factor-1alpha were confirmed by expression of alphaM or alphaL in alphaL-deficient Jurkat cells. Moreover, expression of chimeras containing alphaL and alphaM cytoplasmic domain exchanges indicated that alpha cytoplasmic tails conferred the specific mode of regulation. Coexpressing alphaM or chimeras in mutant Jurkat cells with a "gain of function" phenotype that results in constitutively active LFA-1 demonstrated that Mac-1 was not constitutively active, whereas constitutive activity was mediated via the alphaL cytoplasmic tail, implying the presence of distinct signaling pathways for LFA-1 and Mac-1. Transendothelial chemotaxis of monocytes in response to MCP-1 was dependent on LFA-1; however, Mac-1 was involved at MCP-1 concentrations stimulating its avidity, showing differential contributions of beta2 integrins. Our data suggest that a specific regulation of beta2 integrin avidity by chemokines may be important in leukocyte extravasation and may be triggered by distinct activation pathways transduced via the alpha subunit cytoplasmic domains.  (+info)

Lipopolysaccharide-coated erythrocytes activate human neutrophils via CD14 while subsequent binding is through CD11b/CD18. (8/1480)

Interaction of LPS with monocytes and neutrophils is known to occur via CD14 and is strongly enhanced by LPS-binding protein (LBP). Integrins as well as CD14 play a role in the interaction of erythrocytes (E) coated with LPS or whole Gram-negative bacteria with phagocytes. We reasoned that the density of LPS on a particle is an important determinant in these interactions. Therefore, E were coated with different concentrations of LPS (ELPS). The binding of these ELPS to neutrophils was evaluated by flow cytometry. Simultaneously, we measured fMLP receptor expression to evaluate neutrophil activation. ELPS only bound to neutrophils in the presence of LBP. Blocking CD14 inhibited both activation and binding, whereas blocking complement (C) receptor 3 (CR3) inhibited binding but not activation. TNF activation restored ELPS binding in CD14-blocked cells but not in cells in which CR3 was blocked. Salmonella minnesota did bind to neutrophils independent of CR3 or CD14. The addition of LBP enhanced binding twofold, and this surplus was dependent upon CD14 but not on CR3. We conclude that ELPS interact with neutrophils via CD14, initially giving rise to cell activation; subsequently, binding is solely mediated by activated CR3.  (+info)

Our data demonstrate that an alternative approach to inhibiting leukocyte migration by enhancing the activation of integrins with small molecules is highly effective in reducing leukocyte infiltration and subsequent inflammation in vivo (Fig. 5). We used a cell-based HTS assay (22, 24) to identify and optimize small-molecule agonists of CD11b/CD18, which we have termed leukadherins. The leukadherin compounds LA1 to LA3 have similar chemical structures, and they bind to the ligand-binding αA domain and convert CD11b/CD18 into its active conformation. We showed that leukadherins, but not the structurally similar compound LA-C, promoted CD11b/CD18-dependent cell adhesion and decreased leukocyte motility, which led to a substantial reduction in leukocyte TEM and recruitment into tissues.. In addition, we found that leukadherins had a higher affinity for CD11b/CD18 than did a CD11b/CD18 agonist (32), perhaps because of their more rotationally constrained furanyl thiazolidinone central ring ...
Clone REA1074 reacts with mouse and rat CD29 antigen, a 110-120 kDa integrin family member, also known as integrin ß1. Integrins are cell-surface receptors, expressed as heterodimers essential in various processes mediating intercellular or cell-matrix interaction. CD29 associates non‐covalently with the alpha integrins CD49a-f to form the VLA-1 through VLA-6, and CD51 to form αvß1 complexes. CD29 is broadly expressed on various tissues, including leukocytes, endothelial cells, and epithelial cells. Additional information: Clone REA1074 displays negligible binding to Fc receptors. - España
I steeped the grains at 155F for 30 minutes and had to add filtered water to the boil to make up for the wort that was absorbed by the grain. I did this after the steeping had been completed and the grain sack had been remove, just prior to boiling the wort. Once the boil started I added the hops and waited 20 minutes before adding the DME and letting it boil for the remaining 10 minutes. Next time I will add the hops and 1/2 of the DME to the boil at the same time and then add the remaining 1/2 of the DME 10 minutes prior to flameout ...
Leukocyte adhesion deficiency (LAD), is a rare autosomal recessive disorder characterized by immunodeficiency resulting in recurrent infections. LAD is currently divided into three subtypes: LAD1, LAD2, and the recently described LAD3, also known as LAD-1/variant. In LAD3, the immune defects are supplemented by a Glanzmann thrombasthenia-like bleeding tendency. LAD was first recognized as a distinct clinical entity in the 1970s. The classic descriptions of LAD included recurrent bacterial infections, defects in neutrophil adhesion, and a delay in umbilical cord sloughing. The adhesion defects result in poor leukocyte chemotaxis, particularly neutrophil, inability to form pus and neutrophilia. Individuals with LAD suffer from bacterial infections beginning in the neonatal period. Infections such as omphalitis, pneumonia, gingivitis, and peritonitis are common and often life-threatening due to the infants inability to properly destroy the invading pathogens. These individuals do not form ...
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The disease-gene associations are derived from automatic text mining of the biomedical literature, manually curated database annotations, cancer mutation data, and genome-wide association studies. The confidence of each association is signified by stars, where ★★★★★ is the highest confidence and ★☆☆☆☆ is the lowest.. Developed by Sune Frankild, Albert Pallejà, Kalliopi Tsafou, and Lars Juhl Jensen from the Novo Nordisk Foundation Center for Protein Research.. ...
CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component ...
CD11c is a member of the leukocyte integrin family of adhesion proteins. t is reported to be expressed in normal tissues, mainly on myeloid cells eg.
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We have previously reported a newly discovered congenital disorder of neutrophil adhesion, leukocyte adhesion deficiency syndrome type 2 (LAD II). The clinical manifestations of this syndrome are similar to those seen in the classic leukocyte adhesion deficiency syndrome, now designated type 1 (LAD I), but the two syndromes differ in the molecular basis of their adhesion defects. LAD I is caused by a deficiency in the CD18 integrin adhesion molecules while LAD II patients are deficient in expression of sialyl-Lewis X (SLeX), a carbohydrate ligand for selectins. In this report we demonstrate that neutrophils from a LAD II patient bind minimally or not at all to recombinant E-selectin, purified platelet P-selectin, or P-selectin expressed on histamine-activated human umbilical vein endothelial cells, but have normal levels of L-selectin and CD11b/CD18 integrin, and adhere to and migrate across endothelium when CD11b/CD18 is activated. We compare LAD I and LAD II patient neutrophil function in ...
Leukocyte adhesion deficiency type I (LAD I) is a failure to express CD18, which composes the common ß2 subunit of LFA1 family (ß2 integrins ...
Macrophage infiltration is a hallmark of psoriasis. In the psoriasiform skin disease, activated macrophages were significantly increased in lesional skin as well as in inflamed skin draining lymph nodes (DLNs) of affected CD18hypo mice and were identified to be an important source of TNF-alpha. In vivo both depletion of macrophages and neutralization of TNF-alpha resulted in a significant reduction of the psoriasiform dermatitis. The injection of combination of recombinant JE/MCP-1 and recombinant TNF-alpha elicited skin lesion in healthy PL/J CD18hypo mice. To shed light on the mechanisms that mediate Treg cells suppression of autoimmunity, the functions of Treg and Tresp cells were examined in CD18hypo mice. Low expression of CD18 is responsible for the disruption of cell-cell contacts between dendritic cells and CD4+CD25+ Treg cells. This disruption of cell-cell contacts results in an impaired suppressor function of CD4+CD25+ Treg cells on pathogenic responder T cells. Reduced expression of ...
ITGB2 Full-Length MS Protein Standard (NP_001120963), Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine, was produced in human 293 cells (HEK293) with fully chemically defined cell culture medium to obtain incorporation efficiency at Creative-Proteomics. The product of this gene belongs to the integrin beta chain family of proteins. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. This gene encodes the integrin beta chain beta 2. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Defects in this gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the same protein have been identified for this gene.
ADH-503 | CD11b/CD18 agonist | Leukadherin-1 choline salt | LA1 | ADH503 | ADH 503 | CAS [2055362-74-6] - [2055362-72-4] | Axon 3048 | Axon Ligand™ with >99% purity available from supplier Axon Medchem, prime source of life science reagents for your resea
System C Primer, 2Nd.Ed. P/B. (Cd Included) by Bhasker.J. and a great selection of similar Used, New and Collectible Books available now at AbeBooks.com.
Leukocyte Adhesion Deficiency type I (LAD-I) is a primary immune deficiency caused by mutations in the CD18 subunit of β2 integrins. Affected individuals suffer from recurrent mucocutaneous infections and pathologic inflammation in certain mucosal barriers, including the periodontium, skin and the colon. Our laboratory has recently dissected the mechanistic basis of LAD-I-associated periodontitis but the mechanisms underlying LAD-I- associated colitis are yet to be defined. To answer this question, we utilized as model the CD18−/− mice after demonstrated that CD18 deficiency renders mice highly susceptible to Citrobacter rodentium-induced colitis, a widely used model of human colitis caused by enteropathogenic and enterohaemorrhagic Escherichia coli. Strikingly, we found that CD18−/− mice displayed significantly reduced IL-22 production and IL-22 producing Group 3 innate lymphoid cells (ILC3s). Therapeutic delivery of recombinant IL-22 (rIL-22) protected CD18−/− mice from C. ...
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The cord should separate by about day 8 but delayed separation has been linked with an increased incidence of omphalitis as well as a number of immune disorders such as leucocyte adhesion deficiency.. Immediate versus delayed cord clamping. When the baby is delivered about a third of the fetal-placental blood remains in the placenta (around 30 mls/kg) so with delayed cord clamping the infant gains around 25-30mls/kg of blood via a placental transfusion. The majority of this transfusion occurs within a minute if the neonate is held at the level of the uterus and is almost complete by three minutes.. A recent Cochrane review suggested that delayed cord clamping (by 1 to 3 minutes) is likely to be beneficial as long as access to treatment for jaundice requiring phototherapy is available. This review suggested no difference in adverse events such as severe maternal post-partum haemorrhage, low Apgar scores or worsening neonatal mortality figures when compared to immediate clamping. There did ...
Shop Leukocyte surface antigen ELISA Kit, Recombinant Protein and Leukocyte surface antigen Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Recognition of bacterial lipopolysaccharide (LPS) by the innate immune system elicits strong pro-inflammatory responses that can eventually cause a fatal sepsis syndrome in humans. LPS-mediated activation of mammalian cells is believed to involve the interaction of LPS with lipopolysaccharide-binding protein (LBP) in the serum and, subsequently with CD14. Although there is no doubt that CD14 binds LPS, CD14 is not capable of initiating a transmembrane activation signal because it is a glycosylphosphatidylinositol (GPI)-anchored protein. Accumulating evidence has suggested that LPS must interact with a transmembrane receptor(s) that is responsible for signal transduction. Integrins CD11c and/or CD18, Toll-like receptors (TLRs), as well as CD55, have been suggested to serve this function. Recently, we have revealed that a signalling complex of receptors is formed following LPS stimulation, which comprises heat-shock proteins (Hsps) 70 and 90, chemokine receptor 4 (CXCR4) and growth differentiation ...
The leukocyte integrins play a critical role in a number of cellular adhesive interactions during the immune response. We describe here the isolation and characterization of the chicken beta 2 (CD18) subunit, common to the leukocyte integrin family. The deduced 748-amino-acid sequence reveals a transmembrane protein with 65% and 64% identity with its human and murine homologues, respectively. The chicken beta 2 can associate on the cell surface with the human alpha subunit of LFA-1 and yields a hybrid molecule capable of binding to purified ICAM-1 and ICAM-3 ...
|strong|Mouse anti Bovine CD1w3 antibody, clone CC43 |/strong|recognizes the bovine CD1w3 cell surface antigen, a ~44 kDa molecule expressed as a heterodimer with beta 2 microglobulin.|br||br|Bov…
Leukocyte integrins are intimately involved in transient adherence of leukocytes to endothelium and to each other in the processes of extravasation and cell activation. In this study, seven mAb directed against human CD11a and two mAb directed against human CD18, the alpha- and beta-chains of the leukocyte functional Ag-1 molecule, respectively, were analyzed for their ability to inhibit several leukocyte functional Ag-1-mediated interactions. The best blocking mAb in these studies, a rat anti-human CD18, YFC51.1, was subsequently humanized by complementarily-determining region grafting, associated with human C regions and expressed. The humanized mAb was shown to maintain binding for human CD18. Even though the humanized mAb was an IgG1 isotype it still retained the functional blocking characteristics of the rat mAb while failing to mediate cell killing. The IgG1 mAb was unable to bind human Clq and could block but did not mediate antibody-dependent cellular cytotoxicity.
Anogen is a Canadian biopharmaceutical company, who has been producing antibody products for research, diagnostic and therapeutic applications.
CD11c is a member of the leukocyte integrin family of adhesion proteins. t is reported to be expressed in normal tissues, mainly on myeloid cells eg.
Leukocyte adhesion deficiency/congenital disorder of glycosylation IIc (LAD II/CDG IIc) is a genetic disease characterized by a decreased expression of fucose in glycoconjugates, resulting in leukocyte adhesion deficiency and severe morphological and neurological abnormalities. The biochemical defect is a reduced transport of guanosine diphosphate-L-fucose (GDP-L-fucose) from cytosol into the Golgi compartment, which reduces its availability as substrate for fucosyltransferases. The aim of this study was to determine the effects of a limited supply of GDP-L-fucose inside the Golgi on core fucosylation (a1,6-fucose linked to core N-acetylglucosamine [GlcNAc]) of N-linked glycans in LAD II fibroblasts. The results showed that, although [3H]fucose incorporation was generally reduced in LAD II cells, core fucosylation was affected to a greater extent compared with other types of fucosylation of N-linked oligosaccharides. In particular, core fucosylation was found to be nearly absent in biantennary ...
We report that a subpopulation (10%) of the Mac-1 (CD1 1b/CD18) molecules on activated neutrophils mediates adhesion to ICAM-1 and fibrinogen. We describe a novel mAb (CBRM1/5) that binds to an activation-specific neoepitope on a subset of Mac-1 molecules on neutrophils and monocytes after stimulation with chemoattractants or phorobol esters but does not recognize Mac-1 on resting myeloid cells. CBRM1/5 immunoprecipitates a subpopulation of Mac-1 molecules from detergent lysates of neutrophils, binds to immunoaffinity-purified Mac-1, and localizes to the I domain on the alpha chain of Mac-1. Because CBRM1/5 recognizes a fraction of Mac-1 on activated neutrophils, but still blocks Mac-1-dependent adhesion to fibrinogen and ICAM-1, we suggest that only a small subset of Mac-1 molecules is competent to mediate adhesion. ...
Bernard Chera, T.H. , Sing Chana, H. , Kleinb, G. F. , Jabkowskib, J. , Schadenböck-Kranzlc, G. , Zachd, O. , Roca, X., Alex Law, S.K. (2011) A novel 3′ splice-site mutation and a novel gross deletion in leukocyte adhesion deficiency (LAD)-1star, open. Biochemical and Biophysical Research Communications, 404 (4). pp. 1099-1104. ISSN 0006-291X ...
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Abstract. We have recently found that antibodies to L-selectin, the homing receptor on neutrophils, are as effective as those to beta 2-integrin at blocking fo
ls -F Makefile src/ lib/ $ gtags $ ls G* GPATH GRTAGS GTAGS $ global main src/main.c $ (cd src; global main) main.c $ global -x main main 10 src/main.c main (argc, argv) { $ global -f src/main.c main 10 src/main.c main (argc, argv) { func1 55 src/main.c func1() { func2 72 src/main.c func2() { func3 120 src/main.c func3() { $ global -x ^[sg]et set_num 20 lib/util.c set_num(values) { get_num 30 lib/util.c get_num() { $ global -rx set_num set_num 113 src/op.c set_num(32); set_num 225 src/opop.c if (set_num(0) , 0) { $ global strlen $ (cd /usr/src/sys; gtags) $ export GTAGSLIBPATH=/usr/src/sys $ global -a strlen /usr/src/sys/libkern/strlen.c $ (cd /usr/src/lib; gtags) $ GTAGSLIBPATH=/usr/src/lib:/usr/src/sys $ global -a strlen /usr/src/lib/libc/string/strlen.c ...
The laboratory has demonstrated that Rac GTPases are key regulators of the engraftment and mobilization functions of hematopoietic stem cells. Increasing focus has been on the dysregulated function of these key molecular switches in leukemia. Recent data from the Williams laboratory implicates both Rac and RhoH in acute and chronic leukemias and current efforts include development of new inhibitors of these molecular targets and validation studies of these molecules in leukemia model systems. In addition, this basic work has helped to define the molecular abnormalities in two rare immunodeficiency diseases, Leukocyte Adhesion Deficiency Type IV (due to RAC2 mutations) and Epidermodysplasia Verruciformis (due to mutations in RHOH).. Much of the basic information derived from these studies is also being applied to improve the methods of gene transfer into hematopoietic stem cells using retrovirus and lentivirus vectors.. Dr. Williams is serving as sponsor/investigator for a number of human gene ...
TY - JOUR. T1 - Endogenous nitric oxide inhibits neutrophil adherence to lung epithelial cells to modulate interleukin-8 release. AU - Lin, Horng Chyuan. AU - Wang, Chun Hua. AU - Yu, Chih Teng. AU - Hwang, Kuo Shiung. AU - Kuo, Han Pin. PY - 2001/8/3. Y1 - 2001/8/3. N2 - To investigate the effect of neutrophil adherence to epithelial cells on the release of interleukin 8 (IL-8), we measured neutrophil adherence in the presence or absence of IFN-γ+TNF-α+IL-1β (cytomix) stimulation on cultured A549 epithelial cells. The extent of neutrophil adherence to A549 epithelial cells was measured and the concomitant production of IL-8 and nitrite were assayed. The roles of adhesion molecules and nitrite in modulation of neutrophil adherence were examined by pretreatment with oversaturating ICAM-1 blocking antibody and L-NAME (1mM), respectively. There was a time-dependent spontaneous and cytomix-induced release of IL-8 from epithelial cells, as well as a time-dependent increase in the magnitude of ...
Définitions de 1 3 alpha l fucosidase, synonymes, antonymes, dérivés de 1 3 alpha l fucosidase, dictionnaire analogique de 1 3 alpha l fucosidase (anglais)
Is TNF-α Inhibitor-Induced Psoriasiform Dermatitis Always Psoriasiform? J Drugs Dermatol. 2020 Oct 01;19(10):1009-1010 Authors: Wei C, Murphy EC, Zahn J, Friedman AJ PMID: 33026774 [PubMed - as supplied by publisher]...
CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component ...
Background CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is...
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3. B 림프구의 분화단계에 따른 세포막 단백질 ∎ CD(cluster of differentiation) ※ CD19 : 성숙 B 세포의 표면에 지속적으로 존재하는항원 ( panmarker ). 4. 성숙 B cell 의 활성화 ∎ 활성화된 B cell - plasma cell( IgM ) 로 분화 - ...
αvβ3 integrin-dependent invasion of C8161.9 cells. A) Cell surface expression levels of αvβ3 (clear bar) and αvβ5 (black bar) integrins in two metastatic
The complement system exerts many of its effects through complement receptors (CRs). Of the 8 plasma membrane receptors for complement, only deficiencies of CR3 and CR4 due to CD18 deficiency have been described, known as leukocyte adhesion deficiency (LAD) type 1.
Integrin-dependent adhesions are mechanosensitive constructions in which talin mediates a linkage to actin filaments either directly or indirectly by recruiting vinculin. unique functions in spatial variations and tightness sensing. Overall these total results shed fresh light about talin function and constrain models for cellular mechanosensing. Launch Integrins connect the ECM towards the actin cytoskeleton through a complicated group of linkages where the cytoskeletal proteins talin has a prominent function (Ziegler et al. 2008 Calderwood et al. 2013 The N-terminal FERM (or mind) domains of talin binds right to integrin β subunit cytoplasmic domains and is necessary for conformational activation of integrins to bind ECM proteins with high affinity. Talin includes three F-actin-binding sites (ABSs) using the considerably C-terminal-binding site in the fishing rod domain Stomach muscles3 generally regarded as the main. The talin fishing rod domain also includes multiple binding sites for ...
mouse knock-out or mouse --, human knock-in mutations. Combinatorial epitopes involving residues distant in the sequence provide support for a specific alignment between the beta-subunit and I domains that was used to construct a three-dimensional model. Antigenic residues 133, 332, and 339 are on the first and last predicted alpha-helices of the I-like domain, which are adjacent on its \front.\ Other antigenic residues in beta2 and in other integrin beta subunits are present on the front. No antigenic residues are present on the \back\ of the domain, which is predicted to be in an interface with other domains, such as the alpha subunit beta-propeller domain. Most mutations in the beta2 subunit in leukocyte adhesion deficiency are predicted to be buried in the beta2 subunit I-like domain. Two long insertions are present relative to alpha-subunit I-domains. One is tied down to the back of the I-like domain by a disulfide bond. The other corresponds to the \specificity-determining loop\ ...
Although it has previously been reported that resident macrophages disappear from the inflamed site within the first hour of thioglycollate injection to the peritoneum, resident macrophages do participate in the production of proinflammatory agonists (such as TNF-α, GM-CSF, IL-8, and other chemokines) that activate neutrophils (57, 58). We reported previously that neutrophil influx into the peritoneum is increased in response to thioglycollate in Lsp1−/− mice which harbor increased levels of resident peritoneal macrophages. Therefore, it was important to study the role of LSP1 in neutrophil migration in vivo, independently from changes in resident cell levels to distinguish between intrinsic vs environmental factors influencing the migration of activated neutrophils. In this study, we found that in unstimulated knee joints the number of the synoviocytes was indistinguishable between the two genotypes while the accumulation in the synovial fluid of Lsp1−/− neutrophils was significantly ...
A panel of 21 alpha-subunit (CD11a) and 10 beta-subunit (CD18) anti-LFA-1 mAbs was screened for ability to activate LFA-1. A single anti-CD11a mAb, MEM-83, was identified which was able to directly induce the binding of T cells to purified ICAM-1 immobilized on plastic. This ICAM-1 binding could be achieved by monovalent Fab fragments of mAb MEM-83 at concentrations equivalent to whole antibody, was associated with appearance of the activation reporter epitope detected by mAb 24, and was completely inhibited by anti-ICAM-1 and LFA-1 blocking mAbs. The epitope recognized by mAb MEM-83 was distinct from that recognized by mAb NKI-L16, an anti-CD11a mAb previously reported to induce LFA-1 activation, in that it was constitutively present on freshly isolated peripheral blood mononuclear cells and was not divalent cation dependent for expression. The ICAM-1 binding activity induced by mAb MEM-83 was, however, dependent on the presence of Mg2+ divalent cations. Using an in vitro-translated CD11a ...
ACROBiosystems provides a unique set of CD47-relevant products, including mutilple avi tag pre-biotinylated proteins, for rapid high throughput screening.
Effect of blocking αvβ3 integrins on tenascin- C-dependent smooth muscle cell morphology, attachment efficiency, and survival. (A) Representative phase c
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The I domain of lymphocyte function-associated antigen (LFA)-1 contains an intercellular adhesion molecule (ICAM)-1 and ICAM-3 binding site, but the relationship of this site to regulated adhesion is unknown. To study the adhesive properties of the LFA-1 I domain, we stably expressed a GPI-anchored form of this I domain (I-GPI) on the surface of baby hamster kidney cells. I-GPI cells bound soluble ICAM-1 (sICAM-1) with a low avidity and affinity. Flow cell experiments demonstrated a specific rolling interaction of I-GPI cells on bilayers containing purified full length ICAM-1 or ICAM-3. The LFA-1 activating antibody MEM-83, or its Fab fragment, decreased the rolling velocity of I-GPI cells on ICAM-1-containing membranes. In contrast, the interaction of I-GPI cells with ICAM-3 was blocked by MEM-83. Rolling of I-GPI cells was dependent on the presence of Mg2+. Mn2+ only partially substituted for Mg2+, giving rise to a small fraction of rolling cells and increased rolling velocity. This suggests that the
TY - JOUR. T1 - Supraceliac, but not infrarenal, aortic cross-clamping upregulates neutrophil integrin CD11b. AU - Hill, Gary E.. AU - Mihalakakos, Paul J.. AU - Spurzem, John R.. AU - Baxter, Timothy B.. PY - 1995. Y1 - 1995. N2 - Objective: To evaluate the effects of supraceliac and infrarenal aortic cross-clamping on the expression of neutrophil integrin CD11b (a marker of systemic cytokine release). Design: Two groups, determined by anatomic placement of aortic cross-clamp. Laboratory personnel were blinded as to group assignment. Setting: University teaching and community hospitals. Laboratory facilities used were university and Veterans Affairs medical centers. Participants: Patients scheduled for aortic surgery. Interventions: Blood sampling was performed at baseline, after 30 minutes of aortic cross-clamp duration, 30 and 90 minutes after reperfusion (for tumor necrosis factor-α plasma levels in infrarenal cross-clamp group), and at baseline and 90 minutes reperfusion (for neutrophil ...
Integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide), also known as ITGAL, is a human gene which functions in the immune system. It is involved in cellular adhesion and costimulatory signaling. It is the target of the drug efalizumab.. ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling.[1]. CD11a is one of the two components, along with CD18, which form lymphocyte function-associated antigen-1.. Efalizumab acts as an immunosuppressant by binding to ...
3.Title: Designing multiplex PCR tests for simultaneous screening of bovine leukocyte adhesion deficiency, bovine citrullinemia and factor XI deficiency genetic diseases in cattle. Authors: Anshuman Kumar, Ravi Kumar D, Vineeth MR, Govind Mohan, S Jayakumar, Saket K Niranjan and ID Gupta. Source: Ruminant Science (2017)-6(2):215-220. ...
Neutrophils express receptors for numerous phlogistons which, when occupied, trigger distinct signal-transduction pathways. Previous studies have shown that stimulation of neutrophils with chemoattractants induces shedding of the adhesive molecule L-selectin and increased expression of the beta 2-integrin CD11b/CD18. We determined the effect of ligation of classic, G-protein-linked chemoattractant receptors [C5a, interleukin-8 (IL-8), formylmethionyl-leucylphenylalanine (FMLP) and substance P], receptors for the Fc portion of IgG (Fc gamma receptors) and receptors for transforming growth factor beta (TGF beta) on expression of adhesive molecules by neutrophils and the stimulus-transduction mechanisms thought to mediate these changes. We were surprised to observe that occupancy of Fc gamma receptors by immunocomplexes (BSA-anti-BSA) stimulated increased expression by neutrophils of CD11b/CD18 at concentrations which did not affect L-selectin expression (EC50 9 micrograms/ml versus 350 ...
The leukocyte adhesion cascade is an important paradigm of immunity and mediates leukocyte recruitment in acute or chronic inflammatory responses. Leukocyte recruitment requires several adhesive interactions between leukocytes and endothelial cells. The adhesion of leukocytes to the endothelial cell surface is mediated by interactions between leukocyte integrins, such as the beta1-integrin family member VLA-4 (a4b1) or the beta2-integrin family members LFA-1 (aLb2, CD11a/CD18), Mac-1 (aMb2, CD11b/CD18, complement receptor-3), and their endothelial counter-receptors of the immunoglobulin superfamily (ICAM-1, VCAM-1) (1). Our lab has made significant contributions to the leukocyte adhesion cascade, including the recent identification of a novel endogenous inhibitor of leukocyte recruitment, the endothelial-derived molecule Developmental Endothelial Locus-1 (Del-1, Edil3) (2-4).. Mobilization of hematopoietic stem cells (HSC) from the bone marrow to the periphery takes place upon infection. HSC ...
Given its effects on cell spreading and motility, Rap1 was postulated some time ago to be involved in integrin function; however, confirmation of this required alternative cellular models. Leukocytes represented such a model, since the positive effect of GTP-bound Rap1 on integrin-mediated adhesion is easier to see: in blood cells, in contrast to the more traditional, fibroblast and epithelial, adherent cell systems, integrins are normally kept inactive. Upon inside-out signalling elicited by various agonists, leukocyte integrins can rapidly and transiently be converted to a functionally active, ligand-binding state able to trigger the classic outside-in signalling to Rho-like GTPases (Harris et al., 2000; Schoenwaelder and Burridge, 1999).. Rap1 regulates functional activation of several integrin heterodimers:α 4β1 (VLA-4), α5β1 (VLA-5), αLβ2 (LFA-1, CD11a/CD18), αMβ2 (CR3, CD11b/CD18) and αIIbβ3 (Reedquist et al., 2000; Caron et al., 2000; Katagiri et al., 2000; Arai et al., 2001; ...
Sigma-Aldrich offers abstracts and full-text articles by [G Liu, J T Link, Z Pei, E B Reilly, S Leitza, B Nguyen, K C Marsh, G F Okasinski, T W von Geldern, M Ormes, K Fowler, M Gallatin].
Intercellular Adhesion Molecule-1 information including symptoms, causes, diseases, symptoms, treatments, and other medical and health issues.
A cell-surface ligand involved in leukocyte adhesion and inflammation. Its production is induced by gamma-interferon and it is required for neutrophil migration into inflamed tissue ...
Neutrophils are a key cell type of nonadaptive immune system and are the first phagocytic cell type that reaches mucosal inflammatory sites. On the last stage of their journey from the blood stream to a mucosal surface, neutrophils cross a generally sealed epithelium by migrating along the paracellu …
4F7C: Crystal Structures of Bovine CD1d Reveal Altered αGalCer Presentation and a Restricted A Pocket Unable to Bind Long-Chain Glycolipids.
Additionally tumors can escape antigen-directed therapies by loss or down-regulation of the associated antigens, as well ... doi:10.1158/2159-8290.CD-18-0442. ISSN 2159-8274. PMID 30135176. Upadhyay, Ranjan; Boiarsky, Jonathan A.; Pantsulaia, Gvantsa; ... Different antigens are able to escape through a variety of mechanisms. For example, the African trypanosome parasites are able ... Some antigens may even target pathways different than those the vaccine had originally intended to target. Recent research on ...
CD11a is one of the two components, along with CD18, which form lymphocyte function-associated antigen-1. Efalizumab acts as an ... Integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide), also known as ITGAL, is ... CD11a+Antigen at the US National Library of Medicine Medical Subject Headings (MeSH) ITGAL Info with links in the Cell ... Qu A, Leahy DJ (1995). "Crystal structure of the I-domain from the CD11a/CD18 (LFA-1, alpha L beta 2) integrin". Proc. Natl. ...
"Humanization of the anti-CD18 antibody 6.7: an unexpected effect of a framework residue in binding to antigen". Molecular ... These CDRs are in direct contact with the antigen and are involved in binding antigen, while the framework regions support the ... residues that are in contact with the antigen and those not in contact with the antigen. Framework residues that come in ... If the CDR regions have high affinity for the epitope of antigen, it has been found to be more effective to have a more rigid ...
They are known to bind to leucocyte integrins CD11/CD18 such as LFA-1 and Macrophage-1 antigen, during inflammation and in ...
CD18, CD54). Thus activated Vγ9Vδ2 T cells behave like APCs (γδ T-APC) and present antigens to αβ T cells. This leads to turn ... The antigens recognized by non-Vδ2 T cells expanded in the above infectious contexts have not been characterized, but the fact ... It is still not clear whether these non-peptidic antigens bind directly to the Vγ9/Vδ2 TCR or if a presenting element exists. ... However, none of the known antigen-presenting molecules like MHC class I and II or CD1 are required for γδ T cell activation ...
... and respiratory burst Flow cytometry with monoclonal antibodies is used to screen for deficiencies of CD18. Because the CD18 ... including lymphocyte function-associated antigen 1 (LFA-1), complement receptor 3 (CR-3), and complement receptor 4 (CR-4). The ... This gene encodes CD18, a protein present in several cell surface receptor complexes found on white blood cells, ...
... b/CD18) present on macrophages that is also called Macrophage-1 antigen (CR3) and αMβ2 integrin. CD11c/CD18 also called ... For example, LFA1 (CD11a/CD18) short representation of Lymphocyte Function-associated Antigen 1, also called αLβ2 integrin Mac1 ... In cell biology, CD11 is the α (alpha) component of various integrins, especially ones in which the β (beta) component is CD18 ...
Leukotoxin A: kills granulocytes, monocytes, and other white blood cells expressing integrin beta-2 (CD18) Cytolethal ... cells Inhibition of granulocyte functions Resistant to complement-mediated killing Lipopolysaccharides Surface antigens Heat ...
... antigens, cd8 MeSH D23.050.301.264.894.113 - antigens, cd13 MeSH D23.050.301.264.894.118 - antigens, cd18 MeSH D23.050.301.264. ... antigens, cd14 MeSH D23.050.301.264.035.115 - antigens, cd15 MeSH D23.050.301.264.035.118 - antigens, cd18 MeSH D23.050.301.264 ... antigens, cd14 MeSH D23.101.100.110.115 - antigens, cd15 MeSH D23.101.100.110.118 - antigens, cd18 MeSH D23.101.100.110.119 - ... antigens, cd8 MeSH D23.101.100.894.113 - antigens, cd13 MeSH D23.101.100.894.118 - antigens, cd18 MeSH D23.101.100.894.126 - ...
Binding of CD18 and CD11 results in the formation of Lymphocyte Functions Associated Antigen 1 (LFA-1), a protein found on B ... CD18+antigen at the US National Library of Medicine Medical Subject Headings (MeSH) ITGB2 Info with links in the Cell Migration ... CD18 also exists in soluble, ligand binding forms. Deficiencies in CD18 expression can lead to adhesion defects in circulating ... thus destroying the antigen. In humans, lack of functional CD18 causes Leukocyte Adhesion Deficiency, a disease defined by a ...
CD18 Macrophage-1 antigen (CR3) - Heterodimer: CD11b / CD18 Integrin alphaXbeta2 (CR4) - Heterodimer: CD11c / CD18 Very late ... Antigen Antigenicity Immunogen Superantigen Allergen Hapten Epitope Linear Conformational Mimotope Tumor antigen Antigen- ... ITGB3 Fibrinogen receptor Macrophage-1 antigen (CR3) - Heterodimer: CD11b / CD18 Fibronectin receptor: Integrin alpha2beta1 ... CD18 CR4 - Heterodimer: CD11c / CD18 CRIg (Complement receptor of the immunoglobulin family) Anaphylatoxin receptors C3a ...
Usually, a target cell line expressing a certain surface-exposed antigen is incubated with antibody specific for that antigen. ... These bacteria target the CD18 portion of leukocytes, which has historically been shown to impact ADCC in adhesion-deficient ... ADCC is also important in the use of vaccines, as creation of antibodies and the destruction of antigens introduced to the host ... Frey, Joachim (2019/12). "RTX Toxins of Animal Pathogens and Their Role as Antigens in Vaccines and Diagnostics". Toxins. 11 ( ...
Integrin+alphaM at the US National Library of Medicine Medical Subject Headings (MeSH) Mouse CD Antigen Chart Human CD Antigen ... Todd RF, Petty HR (May 1997). "Beta 2 (CD11/CD18) integrins can serve as signaling partners for other leukocyte receptors". The ... Schymeinsky J, Mócsai A, Walzog B (August 2007). "Neutrophil activation via beta2 integrins (CD11/CD18): molecular mechanisms ... The second chain of αMβ2 is the common integrin β2 subunit known as CD18, and integrin αMβ2 thus belongs to the β2 subfamily ( ...
LAD1 is caused by low expression of CD11 and CD18. CD18 is found on chromosome 21 and CD11 is found on chromosome 16. Leukocyte ... "Lymphocyte function-associated antigen 1 (LFA-1): a surface antigen distinct from Lyt-2,3 that participates in T lymphocyte- ... Lymphocyte function-associated antigen 1 (LFA-1) is an integrin found on lymphocytes and other leukocytes. LFA-1 plays a key ... The antigen that bound to the monoclonal antibodies was identified as an important molecule in cellular recognition processes. ...
... (hereafter complement receptor 3 or CR3) (CD11b/CD18) is a human cell surface receptor found on B and T ... CR3 CD11b/CD18 Macrophage 1 antigen (Mac-1) Macrophage Todd R (1996). "The continuing saga of complement receptor type 3 (CR3 ... Macrophage-1 antigen (or integrin αMβ2 or macrophage integrin or Mac-1) is a complement receptor ("CR3") consisting of CD11b ( ... Wagner C, Hänsch GM, Stegmaier S, Denefleh B, Hug F, Schoels M (April 2001). "The complement receptor 3, CR3 (CD11b/CD18), on T ...
It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by rhinovirus as a receptor for entry into ... Katz FE, Parkar M, Stanley K, Murray LJ, Clark EA, Greaves MF (Jan 1985). "Chromosome mapping of cell membrane antigens ... Huang C, Springer TA (Aug 1995). "A binding interface on the I domain of lymphocyte function-associated antigen-1 (LFA-1) ... ICAM-1 has been shown to interact with CD11a, EZR and CD18. GRCh38: Ensembl release 89: ENSG00000090339 - Ensembl, May 2017 ...
This antigen along with other blood group antigens was used to identify the Basque people as a genetically separate group.[49] ... Because the Duffy antigen is uncommon in those of Black African descent, the presence of this antigen has been used to detect ... The Fy4 antigen, originally described on Fy (a-b-) RBCs, is now thought to be a distinct, unrelated antigen and is no longer ... The Duffy antigen is expressed in greater quantities on reticulocytes than on mature erythrocytes.[21] While the Duffy antigen ...
"Entrez Gene: ITGB3 integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)".. *^ May, K. E.; Villar, J.; Kirtley, S.; ... CD61+Antigens at the US National Library of Medicine Medical Subject Headings (MeSH) ...
... antigens, cd18 MeSH D12.776.543.750.705.408.200.500 - antigens, cd29 MeSH D12.776.543.750.705.408.200.750 - integrin beta3 MeSH ... antigen, b-cell MeSH D12.776.543.750.705.816.821.500 - antigens, cd79 MeSH D12.776.543.750.705.816.824 - receptors, antigen, t- ... antigens, cd22 MeSH D12.776.543.550.200.124 - antigens, cd24 MeSH D12.776.543.550.200.131 - antigens, cd31 MeSH D12.776.543.550 ... antigens, cd27 MeSH D12.776.543.750.705.852.760.072 - antigens, cd30 MeSH D12.776.543.750.705.852.760.097 - antigens, cd40 MeSH ...
LFA-1 (CD11a+CD18). *Integrin alphaXbeta2 (CD11c+CD18). *Macrophage-1 antigen (CD11b+CD18) ...
LFA-1 (CD11a+CD18). *Integrin alphaXbeta2 (CD11c+CD18). *Macrophage-1 antigen (CD11b+CD18) ... "Interaction of glycogen synthase kinase 3beta with the DF3/MUC1 carcinoma-associated antigen and beta-catenin". Molecular and ...
LFA-1 (CD11a+CD18). *Integrin alphaXbeta2 (CD11c+CD18). *Macrophage-1 antigen (CD11b+CD18) ... Primarily, the VCAM-1 protein is an endothelial ligand for VLA-4 (Very Late Antigen-4 or integrin α4β1) of the β1 subfamily of ...
LFA-1 (CD11a+CD18). *Integrin alphaXbeta2 (CD11c+CD18). *Macrophage-1 antigen (CD11b+CD18) ...
LFA-1 (CD11a+CD18). *Integrin alphaXbeta2 (CD11c+CD18). *Macrophage-1 antigen (CD11b+CD18) ... In humans, the CD44 antigen is encoded by the CD44 gene on Chromosome 11.[5] CD44 has been referred to as HCAM (homing cell ... The CD44 antigen is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. ... Indian blood group system at BGMUT Blood Group Antigen Gene Mutation Database at NCBI, NIH ...
... has been shown to interact with: Androgen receptor, BRCA1, CTNNB1, CD18, CD29, CD49c, CREB1, EIF6, FHL3, IGFBP5, ITGA7, ... rise in circulatory prostate-specific antigen (PSA) levels after surgical or radiography treatment) FHL2 expression is ...
It was originally named theta (θ) antigen, then Thy-1 (THYmocyte differentiation antigen 1) due to its prior identification in ... It has been shown to interact with the leukocyte integrin Mac1 (CD11b/CD18) and may play a role in leukocyte homing and ... The antigen Thy-1 was the first T cell marker to be identified. Thy-1 was discovered by Reif and Allen in 1964 during a search ... Reif AE, Allen JM (1964). "The AKR thymic antigen and its distribution in leukemias and nervous tissue". J. Exp. Med. 120 (3): ...
1995). "CD23 regulates monocyte activation through a novel interaction with the adhesion molecules CD11b-CD18 and CD11c-CD18 ... Human CD Antigen Chart. *ITGAX Info with links in the Cell Migration Gateway ... A cadea alfa X de integrina combínase coa cadea beta 2 (CD18 ou ITGB2) para formar unha integrina específica de leucocitos ... Bilsland CA, Diamond MS, Springer TA (1994). "The leukocyte integrin p150,95 (CD11c/CD18) as a receptor for iC3b. Activation by ...
... antigen is a protein that, in humans, is encoded by the CD63 gene. CD63 is mainly associated with membranes of ... Skubitz KM, Campbell KD, Iida J, Skubitz AP (October 1996). "CD63 associates with tyrosine kinase activity and CD11/CD18, and ... Hotta H, Miyamoto H, Hara I, Takahashi N, Homma M (May 1992). "Genomic structure of the ME491/CD63 antigen gene and functional ... Metzelaar MJ, Wijngaard PL, Peters PJ, Sixma JJ, Nieuwenhuis HK, Clevers HC (February 1991). "CD63 antigen. A novel lysosomal ...
Collecting lymphatic vessel permeability facilitates adipose tissue inflammation and distribution of antigen to lymph node- ... role of intercellular adhesion molecule 1 and the CD11/CD18 integrins. Journal of Experimental Medicine, 183(2):451-462. PMCID ... are involved in the immune response by acting as a site for macrophages and dendritic cells to uptake antigens. The results ... Minimal differentiation of classical monocytes as they survey steady-state tissues and transport antigen to lymph nodes. ...
CD11c+Antigens at the US National Library of Medicine Medical Subject Headings (MeSH) Mouse CD Antigen Chart Human CD Antigen ... 1995). "CD23 regulates monocyte activation through a novel interaction with the adhesion molecules CD11b-CD18 and CD11c-CD18". ... Bilsland CA, Diamond MS, Springer TA (1994). "The leukocyte integrin p150,95 (CD11c/CD18) as a receptor for iC3b. Activation by ... Valentin A, Lundin K, Patarroyo M, Asjö B (1990). "The leukocyte adhesion glycoprotein CD18 participates in HIV-1-induced ...
CD18 • CD19 • CD20 • CD21 • CD22 • CD23 • CD24 • CD25 • CD26 • CD27 • CD28 • CD29 • CD30 • CD31 • CD32 (A, B) • CD33 • CD34 • ... 2000). "Characterization of a new member of the TNF family expressed on antigen presenting cells.". Biol. Chem. 380 (12): 1443- ... "BLyS receptor signatures resolve homeostatically independent compartments among naïve and antigen-experienced B cells.". Semin ...
I. Partial characterization of soluble Ki-1 antigen and detection of the antigen in cell culture supernatants and in serum by ... Josimovic-Alasevic O, Dürkop H, Schwarting R, Backé E, Stein H, Diamantstein T (Jan 1989). "Ki-1 (CD30) antigen is released by ... CD30+Antigens at the US National Library of Medicine Medical Subject Headings (MeSH) ... results from cDNA cloning and sequence comparison of the CD30 antigen from different sources". Molecular Immunology. 31 (17): ...
CD18 (Erlizumab) • CD20 (Afutuzumab, Ocrelizumab, Pascolizumab) • CD23 (Lumiliksimab) • CD40 (Teneliksimab, Toralizumab) • ... Induction of Potent and Long-Lasting T-Cell Responses against Cancer Antigens". Cancer Research 62: 1477-1480. ... "A divalent major histocompatibility complex/IgG1 fusion protein induces antigen-specific T cell activation in vitro and in ...
Seligman P. A., Butler C. D., Massey E. J., etal. The p97 antigen is mapped to the q24-qter region of chromosome 3; the same ... Le Beau M. M., Diaz M. O., Plowman G. D., etal. Chromosomal sublocalization of the human p97 melanoma antigen. (англ.) // Hum. ... Plowman G. D., Brown J. P., Enns C. A., etal. Assignment of the gene for human melanoma-associated antigen p97 to chromosome 3 ... Rose T. M., Plowman G. D., Teplow D. B., etal. Primary structure of the human melanoma-associated antigen p97 ( ...
Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) also known as CD66e (Cluster of Differentiation 66e), is a ... 2001). "Heterogeneous RNA-binding protein M4 is a receptor for carcinoembryonic antigen in Kupffer cells". J. Biol. Chem. 276 ( ... CEACAM5, CD66e, CEA, carcinoembryonic antigen related cell adhesion molecule 5. External IDs. HomoloGene: 128801 GeneCards: ... Oikawa S, Nakazato H, Kosaki G (1987). "Primary structure of human carcinoembryonic antigen (CEA) deduced from cDNA sequence". ...
The protein also carries the Jr(a) antigen, which defines the Junior blood group system.[9] ...
van Rhenen A., van Dongen G. A., Kelder A., et al. The novel AML stem cell associated antigen CLL-1 aids in discrimination ...
2002). "CCR6 colocalizes with CD18 and enhances adhesion to activated endothelial cells in CCR6-transduced Jurkat T cells". J. ... Ebert LM, McColl SR (2002). "Up-regulation of CCR5 and CCR6 on distinct subpopulations of antigen-activated CD4+ T lymphocytes ... This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may ... dendritic cells induce antitumor immunity when genetically fused with nonimmunogenic tumor antigens". J. Immunol. 167 (11): ...
"Guiding the selection of human antibodies from phage display repertoires to a single epitope of an antigen". Bio/Technology. 12 ... drug were found by guiding the selection of human antibodies from phage display repertoires to a single epitope of an antigen ...
It is also called Lewis x and SSEA-1 (stage-specific embryonic antigen 1) and represents a marker for murine pluripotent stem ... CD15 Antigen at the US National Library of Medicine Medical Subject Headings (MeSH) ... CD15 (3-fucosyl-N-acetyl-lactosamine) is a cluster of differentiation antigen - an immunologically significant molecule. CD15 ...
A new ligand for human leukocyte antigen class II antigens". The Journal of Experimental Medicine. 176 (2): 327-37. doi:10.1084 ... A new ligand for human leukocyte antigen class II antigens". The Journal of Experimental Medicine. 176 (2): 327-37. doi:10.1084 ... antigen processing and presentation of exogenous peptide antigen via MHC class II. ... antigen binding. • transmembrane signaling receptor activity. • MHC class II protein binding. Cellular component. • membrane. • ...
CD18 • CD19 • CD20 • CD21 • CD22 • CD23 • CD24 • CD25 • CD26 • CD27 • CD28 • CD29 • CD30 • CD31 • CD32 (A, B) • CD33 • CD34 • ... CD97 antigen je protein koji je kod ljudi kodiran CD97 genom.[1][2][3] ... 2001). "Tissue distribution of the human CD97 EGF-TM7 receptor". Tissue Antigens 57 (4): 325-31. PMID 11380941. doi:10.1034/j. ... "Expression cloning and chromosomal mapping of the leukocyte activation antigen CD97, a new seven-span transmembrane molecule of ...
CD18 (Erlizumab) • CD20 (Afutuzumab, Ocrelizumab, Pascolizumab) • CD23 (Lumiliksimab) • CD40 (Teneliksimab, Toralizumab) • ... ćelije bile identifikovane kao najpotentniji proizvođači tipa I interferona u odgovoru na antigen, i bile su nazvani prirodne ...
The serotype or serovar, is a classification of Salmonella into subspecies based on antigens that the organism presents. It is ... Salmonellae are also able to breach the intestinal barrier via phagocytosis and trafficking by CD18-positive immune cells, ... Serotyping is done by mixing cells with antibodies for a particular antigen. It can give some idea about risk. A 2014 study ... and flagellar H antigens (the Kauffman-White classification). The full name of a serotype is given as, for example, Salmonella ...
Nakon presađivanja (transplantacije) organa, telo skoro uvek „odbaci" novi organ usled razlike ljudskih leukocit antigen ... CD18 (Erlizumab) • CD20 (Afutuzumab, Ocrelizumab, Pascolizumab) • CD23 (Lumiliksimab) • CD40 (Teneliksimab, Toralizumab) • ...
"Direct association of adenosine deaminase with a T cell activation antigen, CD26". Science. 261 (5120): 466-9. doi:10.1126/ ...
Past this period CD3 blocks the TCR-antigen binding and causes conformational change or the removal of the entire TCR3/CD3 ... The IL-2a (CD25, T-cell activation antigen, TAC) is expressed only by the already-activated T lymphocytes. Therefore, it is of ... The antilymphocyte (ALG) and antithymocyte antigens (ATG) are being used. They are part of the steroid-resistant acute ... Monoclonal antibodies are directed towards exactly defined antigens. Therefore, they cause fewer side-effects. Especially ...
B cells can present antigens to a specialized group of helper T cells called TFH cells. If an activated TFH cell recognizes the ... Roles of T cell-B-cell-activating molecule (5c8 antigen) and CD40 in contact-dependent help". Journal of Immunology. 149 (12): ... It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In ... Grewal, IS; Xu, J; Flavell, RA (7 December 1995). "Impairment of antigen-specific T-cell priming in mice lacking CD40 ligand". ...
antigen processing and presentation of peptide antigen via MHC class I. • antigen processing and presentation of exogenous ... antigen processing and presentation of exogenous peptide antigen via MHC class I. • lipoprotein transport. • negative ... peptide antigen via MHC class I, TAP-dependent. • platelet degranulation. • MyD88-dependent toll-like receptor signaling ...
... uveitis antigens induce CXCR3- and CXCR5-expressing lymphocytes and immature dendritic cells to migrate (англ.) // Blood (англ ...
In addition to aiding with cytotoxic T cell antigen interactions the CD8 co-receptor also plays a role in T cell signaling. The ... the CD8 co-receptor plays a role in T cell signaling and aiding with cytotoxic T cell antigen interactions. ... This affinity keeps the T cell receptor of the cytotoxic T cell and the target cell bound closely together during antigen- ... Once the T cell receptor binds its specific antigen Lck phosphorylates the cytoplasmic CD3 and ζ-chains of the TCR complex ...
antigen binding. • virus receptor activity. • protein binding. • transmembrane signaling receptor activity. • identical protein ...
CD18 • CD19 • CD20 • CD21 • CD22 • CD23 • CD24 • CD25 • CD26 • CD27 • CD28 • CD29 • CD30 • CD31 • CD32 (A, B) • CD33 • CD34 • ... 1996). "CD88 antibodies specifically bind to C5aR on dermal CD117+ and CD14+ cells and react with a desmosomal antigen in human ...
This change in shape allows the binding of plasma protein Factor B, which allows Factor D to cleave Factor B into Ba and Bb. Bb remains bound to C3(H2O) to form C3(H2O)Bb. This complex is also known as a fluid-phase C3-convertase. This convertase, the alternative pathway C3-convertase, although only produced in small amounts, can cleave multiple C3 proteins into C3a and C3b. The complex is believed to be unstable until it binds properdin, a serum protein. The addition of properdin forms the complex C3bBbP, a stable compound which can bind an additional C3b to form alternative pathway C5-convertase. The C5-convertase of the alternative pathway consists of (C3b)2BbP (sometimes referred to as C3b2Bb). After the creation of C5 convertase (either as (C3b)2BbP or C4b2a3b from the classical pathway), the complement system follows the same path regardless of the means of activation (alternative, classical, or lectin). C5-convertase cleaves C5 into C5a and C5b. C5b binds sequentially to C6, C7, C8 and ...
CD74 (англ. HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain) - мембранный белок ... II histocompatibility antigen gamma chaingamma chain of class II antigensIiHLA-DR antigens-associated invariant chainIa antigen ... Riberdy J.M., Newcomb J.R., Surman M.J., Barbosa J.A., Cresswell P. HLA-DR molecules from an antigen-processing mutant cell ... Machamer C.E., Cresswell P. Biosynthesis and glycosylation of the invariant chain associated with HLA-DR antigens (англ.) // ...
CD18" by people in this website by year, and whether "Antigens, CD18" was a major or minor topic of these publications. ... "Antigens, CD18" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... Below are the most recent publications written about "Antigens, CD18" by people in Profiles. ... Below are MeSH descriptors whose meaning is more general than "Antigens, CD18". ...
Divalent cation substitution reveals CD18- and very late antigen-dependent pathways that mediate human neutrophil adherence to ... Divalent cation substitution reveals CD18- and very late antigen-dependent pathways that mediate human neutrophil adherence to ... Divalent cation substitution reveals CD18- and very late antigen-dependent pathways that mediate human neutrophil adherence to ... Divalent cation substitution reveals CD18- and very late antigen-dependent pathways that mediate human neutrophil adherence to ...
CD18 is the 95 kDa integrin β2-chain which forms non-covalently-bound heterodimers with one of four α subunits. ... CD11/CD18) are the major adhesion molecule family of leukocytes. Most leukocytes express one or more members of this family. ... CD18 Antigen. The beta2 integrins (CD11/CD18) are the major adhesion molecule family of leukocytes. Most leukocytes express one ... CD18 is the 95 kDa integrin β2-chain which forms non-covalently-bound heterodimers with one of four α subunits. The four alpha ...
CD18 is a 90-95 kD type I transmembrane protein also known as integrin β2 subunit, LFA-1 β subunit, and β2 integrin. ... Antigen Details Structure Integrin, type I transmembrane glycoprotein, associates with CD11a, CD11b, or CD11c, 90-95 kD ... CD18 non-covalently associates with CD11a, CD11b or CD11c. CD18 is expressed on all leukocytes. CD18 and associated α chains ... CD18 is a 90-95 kD type I transmembrane protein also known as integrin β2 subunit, LFA-1 β subunit, and β2 integrin. ...
Antigens, CD / immunology* * Brain Edema / immunology * Brain Edema / therapy* * CD18 Antigens * Combined Modality Therapy ... Enhanced attenuation of meningeal inflammation and brain edema by concomitant administration of anti-CD18 monoclonal antibodies ...
White cell differentiation antigens. W. Knapp, and B. Dörken, and W. R. Gilks, and E. P. Rieber, and R. E. Schmidt, and H. ... Surface expression of CD11b and CD18 of imMDCs and maMDCs at day 7. Shaded histograms represent the binding of CD11b- and CD18- ... CD11b/CD18; CR4, CD11c/CD18), and C3d, C3dg (CR2, CD21). HIV-1 infection of human lymphocytes and monocytes/macrophages is ... Cutting Edge: Productive HIV-1 Infection of Dendritic Cells via Complement Receptor Type 3 (CR3, CD11b/CD18). Zsuzsa Bajtay, ...
... a possible CD2/lymphocyte function-associated antigen-1 functional association in humans, Immunology, vol. 99, no. 3, pp. 440- ... T1 - A novel CD18 genomic deletion in a patient with severe leucocyte adhesion deficiency: a possible CD2/lymphocyte function- ... A novel CD18 genomic deletion in a patient with severe leucocyte adhesion deficiency: a possible CD2/lymphocyte function- ... title = "A novel CD18 genomic deletion in a patient with severe leucocyte adhesion deficiency: a possible CD2/lymphocyte ...
This study reports the sequencing of the wild boar beta2-integrin CD11a and CD18 cDNAs. Predicted CD11a and CD18 subunits share ... Besides these strong overall similarities, wild boar and domestic pig LFA-1 differ by 2 (CD18) and 1 or 3 (CD11a) substitutions ... CD11a/CD18, alphaLbeta2), expressed on all leukocytes, is essential for many adhesive functions of the immune system. ... with a larger interspecies conservation for the CD18 than the CD11a. ...
CD11b Antigen / metabolism * CD18 Antigens / genetics * CD18 Antigens / immunology * CD18 Antigens / metabolism ... TDM-induced Mincle signaling on neutrophils increased cell adherence by enhancing F-actin polymerization and CD11b/CD18 surface ... coactivation of the Mincle and TLR2 pathways by TDM and Pam3CSK4 treatment synergistically induced CD11b/CD18 surface ...
Mouse monoclonal CD18 antibody [MEM-48] conjugated to Biotin. Validated in Flow Cyt and tested in Human. Cited in 1 publication ... Ab28093 reacts with an epitope located in the region encoded by aa 534-546 in cysteine rich repeat 3 of the CD18 antigen. ... Anti-CD18 antibody [MEM-48], prediluted (Allophycocyanin) (ab40914) *Anti-CD18 antibody [MEM-48] - Low endotoxin, Azide free ( ... Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte- ...
CD11a/CD18; αL:β2) (T cells) and (ii) the interactions between lymphocyte function-associated antigen 3 (LFA-3; CD58) (DC) and ... Sensitization phase - antigen processing and presentation. The initial step of every specific immune reaction to an antigen (Ag ... Cutaneous lymphocyte antigen is a specialized form of PSGL-1 expressed on skin-homing T cells. Nature 1997: 389: 978-981. * ... Cutting edge: CD1a+ antigen-presenting cells in human dermis respond rapidly to CCR7 ligands. J Immunol 2006: 176: 5730-5734. * ...
Mouse Monoclonal Anti-Integrin beta 2/CD18 Antibody (MEM-48) [Biotin]. Validated: Flow. Tested Reactivity: Human. 100% ... integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1;macrophage antigen 1 (mac-1) beta subunit) ... include CD11a/CD18, CD11b/CD18, CD11c/CD18, and CD11d/CD18. They consist of a common beta subunit (CD18) and homologous alpha ... CD11a/CD18, LFA-1), aMb2 (CD11b/CD18, Mac-1, CR3), aXb2 (CD11c/CD18) and aDb2 (CD11d/CD18). In most cases, the response ...
Rat Monoclonal Anti-Integrin beta 2/CD18 Antibody (YFC118.3) [Janelia Fluor® 549]. Validated: Flow, ICC/IF, IHC, IHC-Fr. Tested ... integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1;macrophage antigen 1 (mac-1) beta subunit) ... include CD11a/CD18, CD11b/CD18, CD11c/CD18, and CD11d/CD18. They consist of a common beta subunit (CD18) and homologous alpha ... This recognises the human CD18 cell surface antigen, the integrin beta2 subunit. CD18 is expressed by the majority of ...
Human Leukocyte Antigen - antigen D Related; HR, heart rate; HRmax, maximum heart rate; HRR, heart rate reserve; HSC, ... CD18, cluster of differentiation 18; CD19, cluster of differentiation 19; CD28, cluster of differentiation 28; CD45RA, cluster ... ACSM, American College of Sports Medicine; APC, antigen presenting cell; CCR7, C-C chemokine receptor type 7; CD4, cluster of ... Dendritic cells differentiate from monocytes to become professional antigen presenting cells (APCs). They form a part of the ...
Buy our Recombinant Human CD18 protein. Ab114283 is a full length protein produced in Wheat germ and has been validated in WB, ... Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C. *Leukocyte cell adhesion molecule CD18 ... 12.5% SDS-PAGE analysis of CD18 protein (ab114283). Stained with Coomassie Blue. ...
Here, we generate a bispecific 4-1BB-agonistic trimerbody targeting the carcinoembryonic antigen (CEA) that is highly expressed ... Here, we generate a bispecific 4-1BB-agonistic trimerbody targeting the carcinoembryonic antigen (CEA) that is highly expressed ... doi: 10.1158/2159-8290.CD-18-0367. PubMed Abstract , CrossRef Full Text , Google Scholar ... CEA is a non-internalizing antigen (17), which may be advantageous compared to other antigens that are rapidly internalized ...
CD18; CELL-FREE SYSTEM; CELLS; CELLULAR STRESSES; CERAMIDE; CHEMOKINE GENE-EXPRESSION; CHROMOSOMAL LOCATION; CLOSTRIDIUM- ... ANTIGEN; ANTINEOPLASTIC AGENTS; ANTIOXIDANTS; ATHEROSCLEROTIC LESION; Autoimmune Diseases (Multiple Sclerosis, Rheumatoid ...
Mac-1: A macrophage differentiation antigen identified by monoclonal antibody. Eur. J. Immunol. 9, 301-306 (1979).. ... Indeed, blocking CD11b/CD18 and its ligands (7-9) and ablation of the genes encoding CD11b (3) or CD18 (10) decrease the ... The integrin CD11b/CD18 (also known as Mac-1), which is a heterodimer of the αM (CD11b) and β2 (CD18) subunits, is critical for ... described an agonist of CD11b/CD18, termed IMB-10, that targets the αA domain of CD11b/CD18 (32). We compared the relative ...
ITGB2 / CD18 (ITGB2 Antibody Abstract) Background Name/Gene ID: ITGB2. Family: Integrin. Synonyms: ITGB2, CD18 antigen, CD18, ... Antigen Integrin beta 2 (ITGB2) Antibodies show synonyms for this antigen * CD18 ... A synthetic peptide corresponding to a sequence at the C-terminus of human CD18(732-748aa LREYRRFEKEKLKSQWN), identical to the ...
The results obtained after incubation of PMN with mAb IB4 (against CD18) suggest that the adhesion of PMN to uninfected ... In turn this activation antigen ELAM-1 binds with its putative ligand present on the PMN membrane. The virus-induced PMN ... Cytomegalovirus induced PMN adherence in relation to an ELAM-1 antigen present on infected endothelial cell monolayers.. Span ... mAb ENA1 reacts with an inducible endothelial surface antigen which has characteristics similar to those of ELAM-1. Incubation ...
Dermal neutrophil emigration in WT and CD18−/− mice 6 h after topical application of 2% croton oil. Emigrated neutrophils were ... Antigens, CD18/biosynthesis/genetics/physiology*. *Cell Movement/immunology*. *Leukocyte-Adhesion Deficiency Syndrome/genetics/ ... or peritoneum of mutant mice deficient in CD18 (CD18-/- mutants). Peripheral blood of CD18-/- mutants contained 11-fold more ... or peritoneum of mutant mice deficient in CD18 (CD18-/- mutants). Peripheral blood of CD18-/- mutants contained 11-fold more ...
ProSpecs CD Antigens include: CD4, CD40, CD10, CD11B, CD14, CD146, CD147, CD1A, CD2, CD21, CD23, CD25, CD29, CD31, CD34, CD38 ...
Protein Aliases: antigen CD11b (p170); CD11 antigen-like family member B; CD11b; CD11B (p170); cell surface glycoprotein MAC-1 ... CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). ... macrophage antigen alpha; macrophage antigen alpha polypeptide; Neutrophil adherence receptor; neutrophil adherence receptor ... CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, ...
Antigen Integrin alpha M (ITGAM) Antikörper Synonyme für dieses Antigen anzeigen * CD11b/CD18 ... Synonyms: CR3A, MO1A, CD11B, MAC-1, MAC1A, SLEB6, Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell ... CD11 antigen-like family member C, Leu M5, Leukocyte adhesion glycoprotein p150,95 alpha chain, Leukocyte adhesion receptor ...
Antigen Integrin alpha M (ITGAM) Antibodies show synonyms for this antigen * CD11b/CD18 ... Synonyms: CR3A, MO1A, CD11B, MAC-1, MAC1A, SLEB6, Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell ... CD11 antigen-like family member C, Leu M5, Leukocyte adhesion glycoprotein p150,95 alpha chain, Leukocyte adhesion receptor ...
CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). ... M1/70 is also cross-reactive to human CD11b, and can be used for the detection of this antigen on human peripheral blood ... CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, ... CD11b non-covalently associates with CD18 to form alphaMbeta2 integrin (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen ...
Leucocytes of patients lacking CD18 or expressing dysfunctional CD18 are defective in chemotaxis, phagocytosis, and homotypic ... CD18 associated integrins bind several ligands including cell surface and extracellular matrix proteins and are involved in ... Clone TS1/18 reacts with CD18, a type I transmembrane protein with an approximate molecular weight of 90-95 kDa. It non- ... Alternative names of antigen. ITGB2, CD18, LAD, LCAMB, LFA-1, Mac-1 α, MF17, MFI7. ...
... also known as lymphocyte function-associated antigen 1 (LFA-1). LFA-1 is a member of the integrin family and consists of two ... chains, a (CD11a) and b2 (CD18). It is expressed on T and B cells as well as on macrophages and neutrophils. LFA-1 plays an ... Clone REA 880 recognizes the CD11a/CD18 heterodimer, also known as lymphocyte function-associated antigen 1 (LFA-1). LFA-1 is a ... Clone REA 880 recognizes the CD11a/CD18 heterodimer, also known as lymphocyte function-associated antigen 1 (LFA-1). LFA-1 is a ...
LAD1 is caused by low expression of CD11 and CD18. CD18 is found on chromosome 21 and CD11 is found on chromosome 16. Leukocyte ... "Lymphocyte function-associated antigen 1 (LFA-1): a surface antigen distinct from Lyt-2,3 that participates in T lymphocyte- ... Lymphocyte function-associated antigen 1 (LFA-1) is an integrin found on lymphocytes and other leukocytes. LFA-1 plays a key ... The antigen that bound to the monoclonal antibodies was identified as an important molecule in cellular recognition processes. ...
... a tumor associated antigen such as HER2, HER3 or HER4 receptor; antibodies against various protein antigens like CD proteins ... anti-CD18 or anti-CD11b antibodies); growth factors such as VEGF; superoxide dismutase; factor; anti-clotting factors such as ... blood group antigens; flk2/flt3 receptor; obesity (OB) receptor; insulin A-chain; insulin B-chain; interleukins (ILs), e.g., IL ... viral antigen such as, for example, a portion of the AIDS envelope, gp120; transport proteins; homing receptors; addressins; ...
  • The four alpha subunits are: CD11a (αL) (all leukocytes), CD11b (αM) (granulocytes, monocytes, some macrophages), CD11c (αX) (granulocytes, monocytes, dendritic antigen presenting cells), CD11d (αD) (CD8 T-cell subpopulation including large granular lymphocytes, macrophages and gamma-delta T cells in splenic red pulp). (beckman.com)
  • CD18 non-covalently associates with CD11a, CD11b or CD11c. (biolegend.com)
  • The most predominant beta 2-integrin lymphocyte function-associated antigen-1 (LFA-1, CD11a/CD18, alpha L beta 2), expressed on all leukocytes, is essential for many adhesive functions of the immune system. (biomedcentral.com)
  • This study reports the sequencing of the wild boar beta 2-integrin CD11a and CD18 cDNAs. (biomedcentral.com)
  • Predicted CD11a and CD18 subunits share all the main structural characteristics of their mammalian homologues, with a larger interspecies conservation for the CD18 than the CD11a. (biomedcentral.com)
  • Besides these strong overall similarities, wild boar and domestic pig LFA-1 differ by 2 (CD18) and 1 or 3 (CD11a) substitutions, of which one is located in the crucial I-domain (CD11a, E168D). (biomedcentral.com)
  • CD18, integrin b2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (b2) integrins: aLb2 (CD11a/CD18, LFA-1), aMb2 (CD11b/CD18, Mac-1, CR3), aXb2 (CD11c/CD18) and aDb2 (CD11d/CD18). (novusbio.com)
  • Clone REA 880 recognizes the CD11a/CD18 heterodimer, also known as lymphocyte function-associated antigen 1 (LFA-1). (miltenyibiotec.com)
  • LFA-1 is a member of the integrin family and consists of two chains, a (CD11a) and b2 (CD18). (miltenyibiotec.com)
  • CD18 is the 95 kDa integrin β2-chain which forms non-covalently-bound heterodimers with the integrin α subunits: CD11a, CD11b and CD11c (αL, αM and αX, respectively). (beckman.com)
  • Shang XZ, Issekutz AC: Contribution of CD11a/CD18, CD11b/CD18, ICAM-1 (CD54) and -2 (CD102) to human monocyte migration through endothelium and connective tissue fibroblast barriers. (exbio.cz)
  • ICAM1 binds to integrins such as CD11a / CD18, or CD11b / CD18. (prospecbio.com)
  • CD11a (LFA-1 alpha, ITGAL) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. (fishersci.com)
  • Three CD11 alpha chains and a common CD18 beta chain form heterodimer transmembrane complexes (CD11a/CD18, CD11b/CD18, CD11c/CD18). (medscape.com)
  • CD11a/CD18) is one of the integrins (β2 integrins) exclusively expressed on leukocytes, and its counterligands are the intercellular adhesion molecules 1, 2, and 3 (ICAM-1, -2, and -3) ( 13 , 35 , 59 ). (asm.org)
  • Lymphocyte function-associated antigen 1 is a heterodimer integrin CD11a (integrin alpha L, p189) and CD18 (integrin beta-2). (brainscape.com)
  • 2 In addition, fluvastatin inhibits the expression of lymphocyte function-associated antigen (CD11a/CD18) and ICAM-1 on human monocytic cell lines. (ahajournals.org)
  • The CD11/CD18 adhesion molecules consist of three heterodimers sharing a common beta subunit (CD18) with a distinct alpha subunit (CD11a, CD11b, CD11c). (fishersci.com)
  • Lymphocyte and monocyte ligands, L-selectin, sialated glycoproteins, lymphocyte function-associated antigen 1 (LFA-1) (CD11a, CD18), and Mac1 (CD11b, CD18) bind to the EC rec and modulate the migration of these cells. (medscape.com)
  • CD11a/CD18) in cytokine-triggered firm leukocyte adhesion to arterial endothelium in vivo. (lu.se)
  • CD11a/CD18) when migrating in response to chemoattractants either across the vasculature into lymph nodes or across stimulated vessels associated with infected tissue. (rupress.org)
  • Interactions between cytotoxic lymphocytes and their targets require the T cell antigen receptor (TCR) and the integrin lymphocyte function-associated molecule-1 (LFA-1, CD11a/CD18). (sciencemag.org)
  • CD11a is the integrin alpha L chain that forms with CD18 the heterodimer molecule Lymphocyte Function-associated Antigen-1 (LFA-1). (peprotech.com)
  • The intercellular adhesion molecules ICAM-1, 2 and 3 are, like VCAM-1, members of the immunoglobulin superfamily (IgSF), but they are recognized by an I domain-containing integrin, lymphocyte-function-associated antigen 1 (LFA-1, or CD11a/CD18). (rcsb.org)
  • i) interaction between lymphocytes, (ii) interaction between T-cells and antigen presenting cells, (iii) adhesion of naïve lymphocytes to post-capillary high endothelial venules of secondary lymphoid tissues, (iv) adhesion of leukocytes to activated endothelium at sites of inflammation for extravasation, (v) control of cell differentiation and proliferation, and (vi) antibody-dependent killing by natural killer cells and granulocytes. (biomedcentral.com)
  • There are currently no images for Integrin beta 2/CD18 Antibody (NBP1-44612). (novusbio.com)
  • In human umbilical vein endothelial cells infected with cytomegalovirus (CMV), an activation antigen recognized by monoclonal antibody (mAb) ENA1 appeared. (nih.gov)
  • This antibody recognizes the antigen presenting on the cell surface membrane of tissue macrophages. (osu.edu)
  • Phospho-CD18 (Ser756/Thr758/759) Antibody Shipping Temperature: HOT Storage Temperature: -20C. (bio-medicine.org)
  • Amplification of Human B Cell Activation by a Monoclonal Antibody to the B Cell-Specific Antigen CD22, Bp 130/140," J. Immunol. (freepatentsonline.com)
  • The antibody MEM-148 recognizes an extracellular epitope on CD18 which is essentially inaccessible in intact integrin molecules on resting leukocytes, but is exposed on high-affinity state of LFA-1 or on unassociated CD18. (exbio.cz)
  • Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD18 (MEM-48) Alexa Fluor® 700 antibody (4 μl reagent / 100 μl of peripheral whole blood). (exbio.cz)
  • Separation of human lymphocytes (red-filled) from human CD18 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD18 (MEM-48) Alexa Fluor® 700 antibody (4 μl reagent / 100 μl of peripheral whole blood). (exbio.cz)
  • Monoclonal antibody to CD18, the beta-chain of LFA-1, did not induce this phenotype. (biomedsearch.com)
  • antibody responses to polysaccharide antigens are decreased and antibody responses to protein antigens are slightly reduced. (medscape.com)
  • Altieri DC, Edgington T (1988) A monoclonal antibody reacting with distinct adhesion molecules defines a transition in the functional state of the receptor CD11b/CD18 (Mac-1). (springer.com)
  • Applications Tested: This 118/A5 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval. (fishersci.com)
  • Based on expression of CD138 and B220, we reveal a unique and major subtype of antigen-specific memory B cells (B220 − CD138 − ) that are distinct from antibody-secreting B cells (B220 +/ −CD138 + ) and B220 + CD138 − memory B cells. (rupress.org)
  • The pattern of cellular differentiation after transfer indicates that B220 − memory B cells act as stable self-replenishing intermediates that arise from B220 + memory B cells and produce antibody-secreting cells on rechallenge with antigen. (rupress.org)
  • Each antibody is crafted with care according to rigorous protocols for immunogen design and preparation, presentation to host animal, and high-affinity purification against the antigen. (abgent.com)
  • In contrast, anti-very late antigen (VLA)-5 mAb antibodies significantly reduced Mn(2+)-mediated PMN adherence to fibronectin, but not to laminin or fibrinogen, demonstrating that VLA-5-mediated PMN adherence to fibronectin, but not to fibrinogen or laminin. (jimmunol.org)
  • These data are consistent with expectations based on studies using blocking antibodies to inhibit CD11/CD18 complexes, and on observations of humans lacking CD11/CD18 complexes. (nih.gov)
  • The antigen that bound to the monoclonal antibodies was identified as an important molecule in cellular recognition processes. (wikipedia.org)
  • However, variation of this coat can lead to antibodies being unable to recognize and eliminate the antigen. (wikipedia.org)
  • CTVT samples were incubated with biotinylated antibodies to specific membrane and cytoplasmic antigens (anti-lysozyme, anti-macrophage, anti-vimentin, anti-CD18, monoclonal anti-CD117, monoclonal anti-CD3, polyclonal anti-CD117, polyclonal CD3 and anti-CD79a), followed by the avidin-biotin-peroxidase complex technique. (scielo.br)
  • There was no immunoreactivity to anti-lysozyme, anti-macrophage, anti-CD18, monoclonal anti-CD117, monoclonal anti-CD3 and anti-CD79a antibodies. (scielo.br)
  • Anti-interleukin (IL)-1α/β and anti-tumor necrosis factor-α antibodies, or anti-lymphocyte function-associated antigen-1 and very late antigen-4 antibodies, had little or no inhibitory effects on MCP-1 secretion by cocultures. (ahajournals.org)
  • Precise cell identification can be achieved by the application of monoclonal antibodies (mAb) specific for leukocyte antigens to isolated cells, cell smears and tissue sections. (vin.com)
  • Argenbright LW, Letts LG, Rothlein R (1991) Monoclonal antibodies to the leukocyte membrane CD18 glycoprotein complex and to intercellular adhesion molecule-1 inhibit leukocyte-endothelial adhesion in rabbits. (springer.com)
  • It involved β2 integrins and Fcγ receptor, because it was prevented by anti-CD18 antibodies and was not observed with anti-PR3 F(ab′) 2 . (asnjournals.org)
  • The CD11c/CD18 complex is expressed on dendritic cells (highly expressed on type 1 myeloid DCs), monocytes, macrophages, granulocytes, NK cells, and subsets of T and B cells. (fishersci.com)
  • CD11c/CD18 signals very late antigen-4 activation to initiate foamy monocyte recruitment during the onset of hypercholesterolemia. (springermedizin.de)
  • The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). (thermofisher.com)
  • On the other hand, trisomy 4 was found in three cases (3.2%) and these cells showed low expressions of CD19 ( P =0.06) and IL-7 receptor ( P =0.05), and high expressions of CD33 ( P =0.13), CD18 ( P =0.03), and CD56 ( P =0.03) when compared to t(8;21) AML without additional karyotypes. (nature.com)
  • Additional derivation of a round cell origin can be made through the use of smooth muscle actin, desmin, Factor VIII-related antigen and glial fibrillary acidic protein (for sarcomas) or staining for specific proteins such as various hormones and hormones receptors such as estrogen receptor. (veterinarypracticenews.com)
  • Antigen standard for integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) (ITGB2), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. (creativebiomart.net)
  • In the case of lymphoid proliferation, assessment of B or T cell antigen receptor rearrangement by polymerase chain reaction (PCR) provides valuable information pertaining to antigen receptor diversity in the lesion. (vin.com)
  • Similarly, B cells are identified by demonstration of the components of the B cell antigen receptor complex (specifically CD79a). (vin.com)
  • Altieri DC, Wiltse WL, Edgington TS (1990b) Signal transduction initiated by extracellular nucleotides regulates the high affinity ligand recognition of the adhesive receptor CD11b/CD18. (springer.com)
  • Recent findings indicate that myeloid-derived suppressor cells can induce antigen-specific CD8 + T-cell tolerance through a posttranslation mechanism which involves modification (nitration) of CD8 and the T-cell receptor itself on the T-cell surface. (aacrjournals.org)
  • MCp concentrations were not altered in the tissues of mice deficient in the alphaE integrin (CD103), the beta2 integrin (CD18), or the recombination activating gene (RAG)-2 gene either alone or in combination with the interleukin (IL)-receptor common gamma chain. (nih.gov)
  • Clonality of the B-cell population could not be demonstrated using PCR testing for antigen receptor gene rearrangement. (tennessee.edu)
  • The integrin CD11b/CD18 (also known as Mac-1), which is a heterodimer of the α M (CD11b) and β 2 (CD18) subunits, is critical for leukocyte adhesion and migration and for immune functions. (sciencemag.org)
  • CD11, along with CD18, form a heterodimer adhesion molecule. (fishersci.com)
  • Patients with leukocyte adhesion molecule (CD11/CD18, beta 2 integrins) deficiency have structural defects in the common beta subunit (CD18), which prevent heterodimer formation and normal cell surface expression of these receptors, leading to life-threatening bacterial infections. (acris-antibodies.com)
  • The beta2 integrins (CD11/CD18) are the major adhesion molecule family of leukocytes. (beckman.com)
  • These receptors are essential for an effective immune system as observed in repeated infections associated with the lymphocyte adhesion deficiency (LAD) type I syndrome, a disease due to mutations in the β 2 (CD18) subunit gene leading to the lack of functional β 2 integrins on the membrane surface of leukocytes [ 12 - 14 ]. (biomedcentral.com)
  • CD18 associated integrins bind several ligands including cell surface and extracellular matrix proteins and are involved in cell-cell and cell-extracellular matrix interactions, immune regulation, and immune tolerance. (miltenyibiotec.com)
  • Tan, S. M. (2012) The leucocyte β2 (CD18) integrins: the structure, functional regulation and signalling properties. (miltenyibiotec.com)
  • Drbal K, Angelisova P, Cerny J, Hilgert I, Horejsi V: A novel anti-CD18 mAb recognizes an activation-related epitope and induces a high-affinity conformation in leukocyte integrins. (exbio.cz)
  • Integrins, specifically leukocyte function associated antigen LFA-1, are cell surface molecules which play a role in T-cell activation. (knowcancer.com)
  • 10. Drbal K, Angelisova P, Hilgert I, Cerny J, Novak P, Horejsi V: A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells. (acris-antibodies.com)
  • Lymphocyte function-associated antigen 1 (LFA-1) is an integrin found on lymphocytes and other leukocytes. (wikipedia.org)
  • Leukocyte function-associated antigen 1 (LFA-1) and CD44 are signalling molecules for cytoskeleton-dependent morphological changes in activated T cells. (biomedsearch.com)
  • Signaling through the leukocyte function-associated antigen 1 (LFA-1) molecule has previously been shown to induce homotypic aggregation in T cells and to induce cytoskeletal changes in T lymphoma cells. (biomedsearch.com)
  • To identify the intracellular signals which increase the adhesiveness of leukocyte function-associated antigen 1 (LFA-1), we established an assay system for activation-dependent adhesion through LFA-1/intercellular adhesion molecule 1 ICAM-1 using mouse lymphoid cells reconstituted with human LFA-1 and then introduced constitutively active forms of signaling molecules. (asm.org)
  • We have investigated the distribution, activity, and cytoskeletal linkage of the integrin lymphocyte function associated antigen-1 (LFA-1) on human T lymphocytes migrating on endothelial cells and on ligand intercellular adhesion molecule-1 (ICAM-1). (rupress.org)
  • Our data demonstrate that CD40 triggering on XG2 cells induced a myeloma cell growth arrest mediated by lymphocyte function-associated antigen 1 and intercellular adhesion molecule 2 interactions. (aacrjournals.org)
  • The lymphocyte function-associated antigen 1 (LFA-1) is a member of the beta2-integrin family and plays a pivotal role for T cell activation and leukocyte trafficking under inflammatory conditions. (mdpi.com)
  • It is only expressed and present on T cells for a short interval occurring after TCR engagement, and has a single identified ligand, 4-1BBL, found on antigen presenting cells ( 4 ). (frontiersin.org)
  • The engagement of 4-1BB by its ligand or agonistic mAbs provides substantial boosts to the T cell response ( 4 ), which prompted the incorporation of 4-1BB intracellular signaling domain into TCR-like chimeric antigen receptors (CARs) and ultimately greatly improved their functionality ( 5 ). (frontiersin.org)
  • In turn this activation antigen ELAM-1 binds with its putative ligand present on the PMN membrane. (nih.gov)
  • A CD Antigen that contains a conserved I domain which is involved in ligand binding. (online-medical-dictionary.org)
  • LFA-1 is not constitutively adhesive, and upregulation of the adhesive activity (avidity) of LFA-1 by external stimuli such as cytokines, chemokines, or antigens is a prerequisite for ligand binding ( 34 , 58 ). (asm.org)
  • We also investigated 4-F-GlcNAc efficacy on lymphocyte E-selectin ligand expression in LNs draining antigen-sensitized skin and on other immunological processes requisite for CHS responses. (jci.org)
  • These data indicate that 4-F-GlcNAc prevents CHS by inhibiting selectin ligand activity and the capacity of effector T cells to enter antigen-challenged skin without affecting the afferent phase of CHS. (jci.org)
  • The antagonistic mAbs used in immune checkpoint blockade are able to block T cell-inhibitory signaling from receptors such as cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) and programmed cell death-1 (PD-1), and have been successfully used in the treatment of several types of cancers ( 1 ). (frontiersin.org)
  • The engagement of the B7 family of molecules on APCs with CD28 and CD152 (cytotoxic T lymphocyte-associated antigen 4 [CTLA-4]) receptors on T cells delivers costimulatory signal(s) important in T cell activation. (rupress.org)
  • Of the 8 plasma membrane receptors for complement, only deficiencies of CR3 and CR4 due to CD18 deficiency have been described, known as leukocyte adhesion deficiency (LAD) type 1 . (medscape.com)
  • This chapter discusses selected scavenger and lectinlike antigen-presenting cell (APC) receptors in relation to innate immunity to illustrate principles and provide questions for further study. (asmscience.org)
  • CD11b non-covalently associates with CD18 to form alphaMbeta2 integrin (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen. (thermofisher.com)
  • CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. (biolegend.com)
  • The results obtained after incubation of PMN with mAb IB4 (against CD18) suggest that the adhesion of PMN to uninfected endothelial cells is CD18 glycoprotein dependent, and virus infection up-regulates this glycoprotein-dependent mechanism. (nih.gov)
  • The virus-induced PMN adhesion occurs also through a CD18 glycoprotein-dependent mechanism. (nih.gov)
  • This is achieved through the switching of its variant surface glycoprotein or VSG, a substance that coats the entire antigen. (wikipedia.org)
  • This recognises the human CD18 cell surface antigen, the integrin beta2 subunit. (novusbio.com)
  • mAb ENA1 reacts with an inducible endothelial surface antigen which has characteristics similar to those of ELAM-1. (nih.gov)
  • CD18 is expressed by all leukocytes (including histiocytes - macrophages, Langerhan's cells and dendritic cells) but no platelets. (beckman.com)
  • Dendritic cells are the most potent antigen presenting cells. (vin.com)
  • In this phase I study, we administered one or two cycles of four triweekly s.c./intradermal injections of ex vivo generated dendritic cells modified with a recombinant fowlpox vector encoding carcinoembryonic antigen (CEA) and a triad of costimulatory molecules [rF-CEA(6D)-TRICOM]. (aacrjournals.org)
  • Because of the crucial role of dendritic cells in adaptive immunity and their potent activity in animal tumor models, numerous pilot studies have evaluated immunotherapy with dendritic cells loaded with antigen in the form of peptide, protein, DNA, mRNA, tumor lysates, tumor fusions, and viral vectors ( 1 ). (aacrjournals.org)
  • To overcome these limitations, we have been studying strategies for genetic modification of dendritic cells with viral vectors encoding full length tumor antigens and costimulatory molecules. (aacrjournals.org)
  • Expression of CD40, CD54, and major histocompatibility complex (MHC) class II HLA-DR antigens by lesional keratinocytes was markedly reduced in serial biopsy specimens. (rupress.org)
  • This is present on both T and B cells and binds to ICAM-1 (CD54) on antigen presenting cells. (brainscape.com)
  • CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). (thermofisher.com)
  • CD18 is a 90-95 kD type I transmembrane protein also known as integrin β 2 subunit, LFA-1 β subunit, and β 2 integrin. (biolegend.com)
  • CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes. (novusbio.com)
  • Clone TS1/18 reacts with CD18, a type I transmembrane protein with an approximate molecular weight of 90-95 kDa. (miltenyibiotec.com)
  • Defects in the CD18 gene results in leukocyte adhesion deficiency. (osu.edu)
  • Divalent cation substitution reveals CD18- and very late antigen-dependent pathways that mediate human neutrophil adherence to fibronectin. (jimmunol.org)
  • Neutrophil emigration in the skin, lungs, and peritoneum: different requirements for CD11/CD18 revealed by CD18-deficient mice. (nih.gov)
  • To determine the role of CD11/CD18 complexes in neutrophil emigration, inflammation was induced in the skin, lungs, or peritoneum of mutant mice deficient in CD18 (CD18-/- mutants). (nih.gov)
  • During Streptococcus pneumoniae pneumonia, neutrophil emigration in CD18-/- mutants was not reduced. (nih.gov)
  • Whereas CD11/CD18 complexes are essential to the dermal emigration of neutrophils during acute dermatitis, CD18-/- mutant mice demonstrate surprising alternative pathways for neutrophil emigration during pneumonia or peritonitis. (nih.gov)
  • Dermal neutrophil emigration in WT and CD18−/− mice 6 h after topical application of 2% croton oil. (nih.gov)
  • Gao JX, Issekutz AC: Mac-1 (CD11b/CD18) is the predominant beta 2 (CD18) integrin mediating human neutrophil migration through synovial and dermal fibroblast barriers. (exbio.cz)
  • In conclusion, this study describes an amplifying loop in polymorphonuclear neutrophil activation process, whereby ANCA are involved in the membrane expression of their own antigen during cell adhesion. (asnjournals.org)
  • Anti-CD18 mAb almost completely blocked adherence to laminin, partly blocked adherence to fibrinogen, but did not inhibit adhesion to fibronectin at all. (jimmunol.org)
  • Triggering CD40 induced an homotypic aggregation of XG2 cells, and the inhibition of proliferation was totally prevented by a blocking anti-CD18 mAb. (aacrjournals.org)
  • Hence, DC in humans and dogs are best defined by their abundant expression of molecules essential to their function as antigen presenting cells. (vin.com)
  • Chatila TA, Geha RS, Arnaout MA (1989) Constitutive and stimulus-induced phosphorylation of CDU/ CD18 leukocyte adhesion molecules. (springer.com)
  • To determine the safety and immunologic and clinical efficacy of a dendritic cell vaccine modified to hyperexpress costimulatory molecules and tumor antigen. (aacrjournals.org)
  • Concentration of MHC class II molecules in lipid rafts facilitates antigen presentation. (springermedizin.de)
  • Here, we generate a bispecific 4-1BB-agonistic trimerbody targeting the carcinoembryonic antigen (CEA) that is highly expressed in cancers of diverse origins. (frontiersin.org)
  • Among the many tumor antigens described, the model antigen, carcinoembryonic antigen (CEA), is of particular interest because it is widely expressed in gastrointestinal, lung, breast, and other malignancies. (aacrjournals.org)
  • The CD11/CD18 complex is part of the beta-2 integrin family and is important in adhesion and phagocytosis (see Table 1). (medscape.com)
  • LAD1 is caused by low expression of CD11 and CD18. (wikipedia.org)
  • LFA-1 is made up of two subunits, known as CD11 and CD18. (knowcancer.com)
  • as are markers for cluster differentiation (CD) antigens such as CD79a (for B cells) and CD3 (for T cells). (veterinarypracticenews.com)
  • These cells constitutively expressed high levels of major histocompatibility complex (MHC) class II antigens but unlike EOC-20 (CRL-2469) expression was not upregulated by recombinant murine interferon-gamma. (atcc.org)
  • CD18 is the 95 kDa integrin β2-chain which forms non-covalently-bound heterodimers with one of four α subunits. (beckman.com)
  • Structural Characterization of the Human B Lymphocyte-Restricted Differentiation Antigen CD22," J. Immunol. (freepatentsonline.com)
  • HD39 (B3), A B Lineage-Restricted Antigen Whose Cell Surface Expression is Limited to Resting and Activated Human B Lymphocytes," J. Immunol. (freepatentsonline.com)
  • Role of the CD22 Human B Cell Antigen in B Cell Triggering by Anti-Immunoglobulin," J. Immunol. (freepatentsonline.com)
  • ADH-503 is an allosteric agonist of integrin CD11b/CD18 (also known as Mac-1) with an EC50 value of 4 mM. (axonmedchem.com)
  • These cells express both myeloid lineage differentiation antigen Gr-1 (Ly6G and Ly6C) and α M integrin CD11b, and in control mice represent ∼20% to 30% of normal bone marrow cells, 2% to 4% of all nucleated splenocytes, and are practically absent in lymph nodes. (aacrjournals.org)
  • The HB-6, CDw75, and CD76 Differentiation Antigens are Unique Cell-Surface Carbohydrate Determinants Generated by the .beta. (freepatentsonline.com)
  • White Cell Differentiation Antigens" McMichael, A. J., Oxford University Press. (freepatentsonline.com)
  • Expression of leukocyte differentiation antigens during the differentiation of HL-60 cells induced b. (biomedsearch.com)
  • TDM-induced Mincle signaling on neutrophils increased cell adherence by enhancing F-actin polymerization and CD11b/CD18 surface expression. (nih.gov)
  • Moreover, coactivation of the Mincle and TLR2 pathways by TDM and Pam3CSK4 treatment synergistically induced CD11b/CD18 surface expression, reactive oxygen species, and TNFα production by neutrophils. (nih.gov)
  • Peripheral blood of CD18-/- mutants contained 11-fold more neutrophils than did blood of wild-type (WT) mice. (nih.gov)
  • During irritant dermatitis induced by topical application of croton oil, the number of emigrated neutrophils in histological sections of dermis was 98% less in CD18-/- mutants than in WT mice. (nih.gov)
  • The number of emigrated neutrophils in lung sections during Escherichia coli pneumonia, or in peritoneal lavage fluid after 4 h of S. pneumoniae peritonitis, was not reduced in CD18-/- mutants, but rather was greater than the WT values (240 +/- 30 and 220 +/- 30% WT, respectively). (nih.gov)
  • Emigrated neutrophils were quantitated morphometrically and expressed as mean ± SEM standardized volume fractions for WT (closed bars) or CD18−/− (open bars) mice. (nih.gov)
  • In contrast, there was no increase in emigrated neutrophils in the dermis of CD18−/− mutant mice after 6 h of croton oil dermatitis (Fig. 2). (nih.gov)
  • CD18 is expressed on all leukocytes. (biolegend.com)
  • and the beta subunit, unique to leukocytes, is beta-2 or CD18. (wikipedia.org)
  • CD18 is expressed by most leukocytes. (osu.edu)
  • Here, we used an alternative strategy of inhibiting leukocyte recruitment by activating CD11b/CD18 with small-molecule agonists, which we term leukadherins. (sciencemag.org)
  • Activation of PMN with phorbol ester or C5a stimulated VLA-5-mediated adhesion to fibronectin, but the contribution of VLA-5 to the forces mediating adherence could only be detected when CD18 function was either blocked with mAb, or when CD18 was congenitally absent. (jimmunol.org)
  • Cytomegalovirus induced PMN adherence in relation to an ELAM-1 antigen present on infected endothelial cell monolayers. (nih.gov)
  • M1/70 is also cross-reactive to human CD11b, and can be used for the detection of this antigen on human peripheral blood monocytes, granulocytes, and a subset of NK cells. (thermofisher.com)
  • 4,5 Lovastatin affects the β2 integrin family of proteins by preventing the formation of CD11b/CD18 heterodimers on monocytes. (ahajournals.org)
  • Flow cytometry analysis (surface staining) of CD18 in human peripheral blood with anti-CD18 (MEM-148) PE. (exbio.cz)
  • Through the recombination of H. pylori's outer membrane proteins, immunoglobulins can no longer recognize these new structures and, therefore, cannot attack the antigen as part of the normal immune response. (wikipedia.org)
  • Some antigens may even target pathways different than those the vaccine had originally intended to target. (wikipedia.org)
  • The interaction with antigen-presenting cells is also a dynamic one in which the T cell crawls across the cell surface in an LFA-1-dependent manner, scanning for antigenic peptides. (rupress.org)
  • Factor VIII-realted antigen (vonWillebrand factor) is present in endothelial cells and in the cytoplasm of megakaryocytes. (osu.edu)
  • These include immune cell attachment to endothelial and antigen-presenting cells, cytotoxicity, and extravasation into tissues. (acris-antibodies.com)