Substances that are recognized by the immune system and induce an immune reaction.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
Differentiation antigens found on thymocytes and on cytotoxic and suppressor T-lymphocytes. CD8 antigens are members of the immunoglobulin supergene family and are associative recognition elements in MHC (Major Histocompatibility Complex) Class I-restricted interactions.
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
Substances elaborated by bacteria that have antigenic activity.
A bifunctional enzyme that catalyzes the synthesis and HYDROLYSIS of CYCLIC ADP-RIBOSE (cADPR) from NAD+ to ADP-RIBOSE. It is a cell surface molecule which is predominantly expressed on LYMPHOID CELLS and MYELOID CELLS.
Glycoproteins found on immature hematopoietic cells and endothelial cells. They are the only molecules to date whose expression within the blood system is restricted to a small number of progenitor cells in the bone marrow.
Differentiation antigens expressed on B-lymphocytes and B-cell precursors. They are involved in regulation of B-cell proliferation.
A member of the tumor necrosis factor receptor superfamily with specificity for CD40 LIGAND. It is found on mature B-LYMPHOCYTES and some EPITHELIAL CELLS, lymphoid DENDRITIC CELLS. Evidence suggests that CD40-dependent activation of B-cells is important for generation of memory B-cells within the germinal centers. Mutations of the gene for CD40 antigen result in HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 3. Signaling of the receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
A membrane glycoprotein and differentiation antigen expressed on the surface of T-cells that binds to CD40 ANTIGENS on B-LYMPHOCYTES and induces their proliferation. Mutation of the gene for CD40 ligand is a cause of HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 1.
Unglycosylated phosphoproteins expressed only on B-cells. They are regulators of transmembrane Ca2+ conductance and thought to play a role in B-cell activation and proliferation.
Substances elaborated by viruses that have antigenic activity.
Costimulatory T-LYMPHOCYTE receptors that have specificity for CD80 ANTIGEN and CD86 ANTIGEN. Activation of this receptor results in increased T-cell proliferation, cytokine production and promotion of T-cell survival.
Acidic sulfated integral membrane glycoproteins expressed in several alternatively spliced and variable glycosylated forms on a wide variety of cell types including mature T-cells, B-cells, medullary thymocytes, granulocytes, macrophages, erythrocytes, and fibroblasts. CD44 antigens are the principle cell surface receptors for hyaluronate and this interaction mediates binding of lymphocytes to high endothelial venules. (From Abbas et al., Cellular and Molecular Immunology, 2d ed, p156)
Differentiation antigens expressed on pluripotential hematopoietic cells, most human thymocytes, and a major subset of peripheral blood T-lymphocytes. They have been implicated in integrin-mediated cellular adhesion and as signalling receptors on T-cells.
Glycolipid-anchored membrane glycoproteins expressed on cells of the myelomonocyte lineage including monocytes, macrophages, and some granulocytes. They function as receptors for the complex of lipopolysaccharide (LPS) and LPS-binding protein.
Glycoprotein members of the immunoglobulin superfamily which participate in T-cell adhesion and activation. They are expressed on most peripheral T-lymphocytes, natural killer cells, and thymocytes, and function as co-receptors or accessory molecules in the T-cell receptor complex.
Ratio of T-LYMPHOCYTES that express the CD4 ANTIGEN to those that express the CD8 ANTIGEN. This value is commonly assessed in the diagnosis and staging of diseases affecting the IMMUNE SYSTEM including HIV INFECTIONS.
Glycoproteins expressed on all mature T-cells, thymocytes, and a subset of mature B-cells. Antibodies specific for CD5 can enhance T-cell receptor-mediated T-cell activation. The B-cell-specific molecule CD72 is a natural ligand for CD5. (From Abbas et al., Cellular and Molecular Immunology, 2d ed, p156)
Antigens expressed primarily on the membranes of living cells during sequential stages of maturation and differentiation. As immunologic markers they have high organ and tissue specificity and are useful as probes in studies of normal cell development as well as neoplastic transformation.
A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.
Glycoproteins expressed on cortical thymocytes and on some dendritic cells and B-cells. Their structure is similar to that of MHC Class I and their function has been postulated as similar also. CD1 antigens are highly specific markers for human LANGERHANS CELLS.
Antibodies produced by a single clone of cells.
The 140 kDa isoform of NCAM (neural cell adhesion molecule) containing a transmembrane domain and short cytoplasmic tail. It is expressed by all lymphocytes mediating non-MHC restricted cytotoxicity and is present on some neural tissues and tumors.
Antigens expressed on the cell membrane of T-lymphocytes during differentiation, activation, and normal and neoplastic transformation. Their phenotypic characterization is important in differential diagnosis and studies of thymic ontogeny and T-cell function.
A membrane-bound or cytosolic enzyme that catalyzes the synthesis of CYCLIC ADP-RIBOSE (cADPR) from nicotinamide adenine dinucleotide (NAD). This enzyme generally catalyzes the hydrolysis of cADPR to ADP-RIBOSE, as well, and sometimes the synthesis of cyclic ADP-ribose 2' phosphate (2'-P-cADPR) from NADP.
Surface antigens expressed on myeloid cells of the granulocyte-monocyte-histiocyte series during differentiation. Analysis of their reactivity in normal and malignant myelomonocytic cells is useful in identifying and classifying human leukemias and lymphomas.
A costimulatory ligand expressed by ANTIGEN-PRESENTING CELLS that binds to CTLA-4 ANTIGEN with high specificity and to CD28 ANTIGEN with low specificity. The interaction of CD80 with CD28 ANTIGEN provides a costimulatory signal to T-LYMPHOCYTES, while its interaction with CTLA-4 ANTIGEN may play a role in inducing PERIPHERAL TOLERANCE.
Tetraspanin proteins found at high levels in cells of the lymphoid-myeloid lineage. CD53 antigens may be involved regulating the differentiation of T-LYMPHOCYTES and the activation of B-LYMPHOCYTES.
A cell adhesion protein that was originally identified as a heat stable antigen in mice. It is involved in METASTASIS and is highly expressed in many NEOPLASMS.
Zinc-binding metalloproteases that are members of the type II integral membrane metalloproteases. They are expressed by GRANULOCYTES; MONOCYTES; and their precursors as well as by various non-hematopoietic cells. They release an N-terminal amino acid from a peptide, amide or arylamide.
Any part or derivative of any protozoan that elicits immunity; malaria (Plasmodium) and trypanosome antigens are presently the most frequently encountered.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
A costimulatory ligand expressed by ANTIGEN-PRESENTING CELLS that binds to CD28 ANTIGEN with high specificity and to CTLA-4 ANTIGEN with low specificity. The interaction of CD86 with CD28 ANTIGEN provides a stimulatory signal to T-LYMPHOCYTES, while its interaction with CTLA-4 ANTIGEN may play a role in inducing PERIPHERAL TOLERANCE.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.
A tumor necrosis factor receptor subtype found in a variety of tissues and on activated LYMPHOCYTES. It has specificity for FAS LIGAND and plays a role in regulation of peripheral immune responses and APOPTOSIS. Multiple isoforms of the protein exist due to multiple ALTERNATIVE SPLICING. The activated receptor signals via a conserved death domain that associates with specific TNF RECEPTOR-ASSOCIATED FACTORS in the CYTOPLASM.
Antigens determined by leukocyte loci found on chromosome 6, the major histocompatibility loci in humans. They are polypeptides or glycoproteins found on most nucleated cells and platelets, determine tissue types for transplantation, and are associated with certain diseases.
Membrane antigens associated with maturation stages of B-lymphocytes, often expressed in tumors of B-cell origin.
High-molecular weight glycoproteins uniquely expressed on the surface of LEUKOCYTES and their hemopoietic progenitors. They contain a cytoplasmic protein tyrosine phosphatase activity which plays a role in intracellular signaling from the CELL SURFACE RECEPTORS. The CD45 antigens occur as multiple isoforms that result from alternative mRNA splicing and differential usage of three exons.
Process of classifying cells of the immune system based on structural and functional differences. The process is commonly used to analyze and sort T-lymphocytes into subsets based on CD antigens by the technique of flow cytometry.
Substances of fungal origin that have antigenic activity.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The major group of transplantation antigens in the mouse.
A 67-kDa sialic acid binding lectin that is specific for MYELOID CELLS and MONOCYTE-MACROPHAGE PRECURSOR CELLS. This protein is the smallest siglec subtype and contains a single immunoglobulin C2-set domain. It may play a role in intracellular signaling via its interaction with SHP-1 PROTEIN-TYROSINE PHOSPHATASE and SHP-2 PROTEIN-TYROSINE PHOSPHATASE.
Any part or derivative of a helminth that elicits an immune reaction. The most commonly seen helminth antigens are those of the schistosomes.
Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.
Cell-surface glycoprotein beta-chains that are non-covalently linked to specific alpha-chains of the CD11 family of leukocyte-adhesion molecules (RECEPTORS, LEUKOCYTE-ADHESION). A defect in the gene encoding CD18 causes LEUKOCYTE-ADHESION DEFICIENCY SYNDROME.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
A member of the tumor necrosis factor receptor superfamily that may play a role in the regulation of NF-KAPPA B and APOPTOSIS. They are found on activated T-LYMPHOCYTES; B-LYMPHOCYTES; NEUTROPHILS; EOSINOPHILS; MAST CELLS and NK CELLS. Overexpression of CD30 antigen in hematopoietic malignancies make the antigen clinically useful as a biological tumor marker. Signaling of the receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
Glycoproteins found on the membrane or surface of cells.
A critical subpopulation of regulatory T-lymphocytes involved in MHC Class I-restricted interactions. They include both cytotoxic T-lymphocytes (T-LYMPHOCYTES, CYTOTOXIC) and CD8+ suppressor T-lymphocytes.
Sites on an antigen that interact with specific antibodies.
A glycoprotein that is secreted into the luminal surface of the epithelia in the gastrointestinal tract. It is found in the feces and pancreaticobiliary secretions and is used to monitor the response to colon cancer treatment.
A subclass of HLA-D antigens that consist of alpha and beta chains. The inheritance of HLA-DR antigens differs from that of the HLA-DQ ANTIGENS and HLA-DP ANTIGENS.
A trisaccharide antigen expressed on glycolipids and many cell-surface glycoproteins. In the blood the antigen is found on the surface of NEUTROPHILS; EOSINOPHILS; and MONOCYTES. In addition, CD15 antigen is a stage-specific embryonic antigen.
Those proteins recognized by antibodies from serum of animals bearing tumors induced by viruses; these proteins are presumably coded for by the nucleic acids of the same viruses that caused the neoplastic transformation.
Established cell cultures that have the potential to propagate indefinitely.
A sialic acid-rich protein and an integral cell membrane mucin. It plays an important role in activation of T-LYMPHOCYTES.
Leukocyte differentiation antigens and major platelet membrane glycoproteins present on MONOCYTES; ENDOTHELIAL CELLS; PLATELETS; and mammary EPITHELIAL CELLS. They play major roles in CELL ADHESION; SIGNAL TRANSDUCTION; and regulation of angiogenesis. CD36 is a receptor for THROMBOSPONDINS and can act as a scavenger receptor that recognizes and transports oxidized LIPOPROTEINS and FATTY ACIDS.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A group of three different alpha chains (CD11a, CD11b, CD11c) that are associated with an invariant CD18 beta chain (ANTIGENS, CD18). The three resulting leukocyte-adhesion molecules (RECEPTORS, LEUKOCYTE ADHESION) are LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN-1; MACROPHAGE-1 ANTIGEN; and ANTIGEN, P150,95.
Large, transmembrane, non-covalently linked glycoproteins (alpha and beta). Both chains can be polymorphic although there is more structural variation in the beta chains. The class II antigens in humans are called HLA-D ANTIGENS and are coded by a gene on chromosome 6. In mice, two genes named IA and IE on chromosome 17 code for the H-2 antigens. The antigens are found on B-lymphocytes, macrophages, epidermal cells, and sperm and are thought to mediate the competence of and cellular cooperation in the immune response. The term IA antigens used to refer only to the proteins encoded by the IA genes in the mouse, but is now used as a generic term for any class II histocompatibility antigen.
A group of antigens that includes both the major and minor histocompatibility antigens. The former are genetically determined by the major histocompatibility complex. They determine tissue type for transplantation and cause allograft rejections. The latter are systems of allelic alloantigens that can cause weak transplant rejection.
Small glycoproteins found on both hematopoietic and non-hematopoietic cells. CD59 restricts the cytolytic activity of homologous complement by binding to C8 and C9 and blocking the assembly of the membrane attack complex. (From Barclay et al., The Leukocyte Antigen FactsBook, 1993, p234)
IMMUNOGLOBULINS on the surface of B-LYMPHOCYTES. Their MESSENGER RNA contains an EXON with a membrane spanning sequence, producing immunoglobulins in the form of type I transmembrane proteins as opposed to secreted immunoglobulins (ANTIBODIES) which do not contain the membrane spanning segment.
Nuclear antigen with a role in DNA synthesis, DNA repair, and cell cycle progression. PCNA is required for the coordinated synthesis of both leading and lagging strands at the replication fork during DNA replication. PCNA expression correlates with the proliferation activity of several malignant and non-malignant cell types.
Oligosaccharide antigenic determinants found principally on NK cells and T-cells. Their role in the immune response is poorly understood.
A transmembrane protein belonging to the tumor necrosis factor superfamily that specifically binds to CD27 ANTIGEN. It is found on activated T-LYMPHOCYTES; B-LYMPHOCYTES; and DENDRITIC CELLS where it plays a role in stimulating the proliferation of CD4-POSITIVE T-LYMPHOCYTES and CD8-POSITIVE T-LYMPHOCYTES.
A ubiquitously expressed complement receptor that binds COMPLEMENT C3B and COMPLEMENT C4B and serves as a cofactor for their inactivation. CD46 also interacts with a wide variety of pathogens and mediates immune response.
A class of animal lectins that bind to carbohydrate in a calcium-dependent manner. They share a common carbohydrate-binding domain that is structurally distinct from other classes of lectins.
Glycoproteins with a wide distribution on hematopoietic and non-hematopoietic cells and strongly expressed on macrophages. CD58 mediates cell adhesion by binding to CD2; (ANTIGENS, CD2); and this enhances antigen-specific T-cell activation.
Spherical phototrophic bacteria found in mud and stagnant water exposed to light.
A ubiquitously expressed membrane glycoprotein. It interacts with a variety of INTEGRINS and mediates responses to EXTRACELLULAR MATRIX PROTEINS.
A CD antigen that contains a conserved I domain which is involved in ligand binding. When combined with CD18 the two subunits form MACROPHAGE-1 ANTIGEN.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A glycoprotein that is a kallikrein-like serine proteinase and an esterase, produced by epithelial cells of both normal and malignant prostate tissue. It is an important marker for the diagnosis of prostate cancer.
An integrin alpha subunit of approximately 150-kDa molecular weight. It is expressed at high levels on monocytes and combines with CD18 ANTIGEN to form the cell surface receptor INTEGRIN ALPHAXBETA2. The subunit contains a conserved I-domain which is characteristic of several of alpha integrins.
The lipopolysaccharide-protein somatic antigens, usually from gram-negative bacteria, important in the serological classification of enteric bacilli. The O-specific chains determine the specificity of the O antigens of a given serotype. O antigens are the immunodominant part of the lipopolysaccharide molecule in the intact bacterial cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
A specific HLA-A surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-A*02 allele family.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Progenitor cells from which all blood cells derive.
The number of CD4-POSITIVE T-LYMPHOCYTES per unit volume of BLOOD. Determination requires the use of a fluorescence-activated flow cytometer.
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
Carbohydrate antigens expressed by malignant tissue. They are useful as tumor markers and are measured in the serum by means of a radioimmunoassay employing monoclonal antibodies.
GPI-linked membrane proteins broadly distributed among hematopoietic and non-hematopoietic cells. CD55 prevents the assembly of C3 CONVERTASE or accelerates the disassembly of preformed convertase, thus blocking the formation of the membrane attack complex.
An equimolar complex of 4,4'-Dinitrocarbanilide and 2-Hydroxy-4,6-dimethylpyrimidine. A coccidiostat for poultry.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Membrane glycoproteins consisting of an alpha subunit and a BETA 2-MICROGLOBULIN beta subunit. In humans, highly polymorphic genes on CHROMOSOME 6 encode the alpha subunits of class I antigens and play an important role in determining the serological specificity of the surface antigen. Class I antigens are found on most nucleated cells and are generally detected by their reactivity with alloantisera. These antigens are recognized during GRAFT REJECTION and restrict cell-mediated lysis of virus-infected cells.
Tetraspanin proteins that are involved in a variety of cellular functions including BASEMENT MEMBRANE assembly, and in the formation of a molecular complexes on the surface of LYMPHOCYTES.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
A member of the tumor necrosis factor receptor superfamily that is specific for 4-1BB LIGAND. It is found in a variety of immune cell types including activated T-LYMPHOCYTES; NATURAL KILLER CELLS; and DENDRITIC CELLS. Activation of the receptor on T-LYMPHOCYTES plays a role in their expansion, production of cytokines and survival. Signaling by the activated receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Proteins prepared by recombinant DNA technology.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.
Polymorphic class I human histocompatibility (HLA) surface antigens present on almost all nucleated cells. At least 20 antigens have been identified which are encoded by the A locus of multiple alleles on chromosome 6. They serve as targets for T-cell cytolytic responses and are involved with acceptance or rejection of tissue/organ grafts.
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).
Receptors present on activated T-LYMPHOCYTES and B-LYMPHOCYTES that are specific for INTERLEUKIN-2 and play an important role in LYMPHOCYTE ACTIVATION. They are heterotrimeric proteins consisting of the INTERLEUKIN-2 RECEPTOR ALPHA SUBUNIT, the INTERLEUKIN-2 RECEPTOR BETA SUBUNIT, and the INTERLEUKIN RECEPTOR COMMON GAMMA-CHAIN.
Sets of cell surface antigens located on BLOOD CELLS. They are usually membrane GLYCOPROTEINS or GLYCOLIPIDS that are antigenically distinguished by their carbohydrate moieties.
Those hepatitis B antigens found on the surface of the Dane particle and on the 20 nm spherical and tubular particles. Several subspecificities of the surface antigen are known. These were formerly called the Australia antigen.
Ubiquitously-expressed tetraspanin proteins that are found in late ENDOSOMES and LYSOSOMES and have been implicated in intracellular transport of proteins.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Tetraspanin proteins found associated with LAMININ-binding INTEGRINS. The CD151 antigens may play a role in the regulation of CELL MOTILITY.
A component of the B-cell antigen receptor that is involved in B-cell antigen receptor heavy chain transport to the PLASMA MEMBRANE. It is expressed almost exclusively in B-LYMPHOCYTES and serves as a useful marker for B-cell NEOPLASMS.
An encapsulated lymphatic organ through which venous blood filters.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Human immune-response or Class II antigens found mainly, but not exclusively, on B-lymphocytes and produced from genes of the HLA-D locus. They are extremely polymorphic families of glycopeptides, each consisting of two chains, alpha and beta. This group of antigens includes the -DR, -DQ and -DP designations, of which HLA-DR is most studied; some of these glycoproteins are associated with certain diseases, possibly of immune etiology.
A membrane-bound tumor necrosis family member found primarily on activated T-LYMPHOCYTES that binds specifically to CD30 ANTIGEN. It may play a role in INFLAMMATION and immune regulation.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
A class of enzymes involved in the hydrolysis of the N-glycosidic bond of nitrogen-linked sugars.
A form of undifferentiated malignant LYMPHOMA usually found in central Africa, but also reported in other parts of the world. It is commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. B-cell antigens are expressed on the immature cells that make up the tumor in virtually all cases of Burkitt lymphoma. The Epstein-Barr virus (HERPESVIRUS 4, HUMAN) has been isolated from Burkitt lymphoma cases in Africa and it is implicated as the causative agent in these cases; however, most non-African cases are EBV-negative.
Molecules on the surface of B- and T-lymphocytes that recognize and combine with specific antigens.
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.
An alpha-integrin subunit found on lymphocytes, granulocytes, macrophages and monocytes. It combines with the integrin beta2 subunit (CD18 ANTIGEN) to form LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN-1.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Antigens of the virion of the HEPATITIS B VIRUS or the Dane particle, its surface (HEPATITIS B SURFACE ANTIGENS), core (HEPATITIS B CORE ANTIGENS), and other associated antigens, including the HEPATITIS B E ANTIGENS.
The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.
The processes triggered by interactions of ANTIBODIES with their ANTIGENS.
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
Mice homozygous for the mutant autosomal recessive gene "scid" which is located on the centromeric end of chromosome 16. These mice lack mature, functional lymphocytes and are thus highly susceptible to lethal opportunistic infections if not chronically treated with antibiotics. The lack of B- and T-cell immunity resembles severe combined immunodeficiency (SCID) syndrome in human infants. SCID mice are useful as animal models since they are receptive to implantation of a human immune system producing SCID-human (SCID-hu) hematochimeric mice.
Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
A heterogeneous group of immunocompetent cells that mediate the cellular immune response by processing and presenting antigens to the T-cells. Traditional antigen-presenting cells include MACROPHAGES; DENDRITIC CELLS; LANGERHANS CELLS; and B-LYMPHOCYTES. FOLLICULAR DENDRITIC CELLS are not traditional antigen-presenting cells, but because they hold antigen on their cell surface in the form of IMMUNE COMPLEXES for B-cell recognition they are considered so by some authors.
The type species of LYMPHOCRYPTOVIRUS, subfamily GAMMAHERPESVIRINAE, infecting B-cells in humans. It is thought to be the causative agent of INFECTIOUS MONONUCLEOSIS and is strongly associated with oral hairy leukoplakia (LEUKOPLAKIA, HAIRY;), BURKITT LYMPHOMA; and other malignancies.
T-cell receptors composed of CD3-associated alpha and beta polypeptide chains and expressed primarily in CD4+ or CD8+ T-cells. Unlike immunoglobulins, the alpha-beta T-cell receptors recognize antigens only when presented in association with major histocompatibility (MHC) molecules.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Class I human histocompatibility (HLA) surface antigens encoded by more than 30 detectable alleles on locus B of the HLA complex, the most polymorphic of all the HLA specificities. Several of these antigens (e.g., HLA-B27, -B7, -B8) are strongly associated with predisposition to rheumatoid and other autoimmune disorders. Like other class I HLA determinants, they are involved in the cellular immune reactivity of cytolytic T lymphocytes.
The altered state of immunologic responsiveness resulting from initial contact with antigen, which enables the individual to produce antibodies more rapidly and in greater quantity in response to secondary antigenic stimulus.
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
The phenomenon of target cell destruction by immunologically active effector cells. It may be brought about directly by sensitized T-lymphocytes or by lymphoid or myeloid "killer" cells, or it may be mediated by cytotoxic antibody, cytotoxic factor released by lymphoid cells, or complement.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
A melanosome-specific protein that plays a role in the expression, stability, trafficking, and processing of GP100 MELANOMA ANTIGEN, which is critical to the formation of Stage II MELANOSOMES. The protein is used as an antigen marker for MELANOMA cells.
A widely distributed cell surface transmembrane glycoprotein that stimulates the synthesis of MATRIX METALLOPROTEINASES. It is found at high levels on the surface of malignant NEOPLASMS and may play a role as a mediator of malignant cell behavior.
A general term for various neoplastic diseases of the lymphoid tissue.
An albumin obtained from the white of eggs. It is a member of the serpin superfamily.
Antigens associated with specific proteins of the human adult T-cell immunodeficiency virus (HIV); also called HTLV-III-associated and lymphadenopathy-associated virus (LAV) antigens.
An inhibitory T CELL receptor that is closely related to CD28 ANTIGEN. It has specificity for CD80 ANTIGEN and CD86 ANTIGEN and acts as a negative regulator of peripheral T cell function. CTLA-4 antigen is believed to play role in inducing PERIPHERAL TOLERANCE.
A promyelocytic cell line derived from a patient with ACUTE PROMYELOCYTIC LEUKEMIA. HL-60 cells lack specific markers for LYMPHOID CELLS but express surface receptors for FC FRAGMENTS and COMPLEMENT SYSTEM PROTEINS. They also exhibit phagocytic activity and responsiveness to chemotactic stimuli. (From Hay et al., American Type Culture Collection, 7th ed, pp127-8)
A widely expressed transmembrane glycoprotein that functions as a METASTASIS suppressor protein. It is underexpressed in a variety of human NEOPLASMS.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
A group of differentiation surface antigens, among the first to be discovered on thymocytes and T-lymphocytes. Originally identified in the mouse, they are also found in other species including humans, and are expressed on brain neurons and other cells.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
The specific failure of a normally responsive individual to make an immune response to a known antigen. It results from previous contact with the antigen by an immunologically immature individual (fetus or neonate) or by an adult exposed to extreme high-dose or low-dose antigen, or by exposure to radiation, antimetabolites, antilymphocytic serum, etc.
Manifestations of the immune response which are mediated by antigen-sensitized T-lymphocytes via lymphokines or direct cytotoxicity. This takes place in the absence of circulating antibody or where antibody plays a subordinate role.
A single, unpaired primary lymphoid organ situated in the MEDIASTINUM, extending superiorly into the neck to the lower edge of the THYROID GLAND and inferiorly to the fourth costal cartilage. It is necessary for normal development of immunologic function early in life. By puberty, it begins to involute and much of the tissue is replaced by fat.
Endogenous tissue constituents that have the ability to interact with AUTOANTIBODIES and cause an immune response.
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
Nuclear antigens encoded by VIRAL GENES found in HUMAN HERPESVIRUS 4. At least six nuclear antigens have been identified.
A soluble substance elaborated by antigen- or mitogen-stimulated T-LYMPHOCYTES which induces DNA synthesis in naive lymphocytes.
A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.
A cell line derived from cultured tumor cells.
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
A sex-specific cell surface antigen produced by the sex-determining gene of the Y chromosome in mammals. It causes syngeneic grafts from males to females to be rejected and interacts with somatic elements of the embryologic undifferentiated gonad to produce testicular organogenesis.
A cell adhesion molecule of the immunoglobulin superfamily that is expressed in ENDOTHELIAL CELLS and is involved in INTERCELLULAR JUNCTIONS.
Antigens stimulating the formation of, or combining with heterophile antibodies. They are cross-reacting antigens found in phylogenetically unrelated species.
CD4-positive T cells that inhibit immunopathology or autoimmune disease in vivo. They inhibit the immune response by influencing the activity of other cell types. Regulatory T-cells include naturally occurring CD4+CD25+ cells, IL-10 secreting Tr1 cells, and Th3 cells.
Antibodies obtained from a single clone of cells grown in mice or rats.
Antigenic determinants recognized and bound by the T-cell receptor. Epitopes recognized by the T-cell receptor are often located in the inner, unexposed side of the antigen, and become accessible to the T-cell receptors after proteolytic processing of the antigen.
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
A heterodimeric protein that is a cell surface antigen associated with lymphocyte activation. The initial characterization of this protein revealed one identifiable heavy chain (ANTIGENS, CD98 HEAVY CHAIN) and an indeterminate smaller light chain. It is now known that a variety of light chain subunits (ANTIGENS, CD98 LIGHT CHAINS) can dimerize with the heavy chain. Depending upon its light chain composition a diverse array of functions can be found for this protein. Functions include: type L amino acid transport, type y+L amino acid transport and regulation of cellular fusion.
The hepatitis B antigen within the core of the Dane particle, the infectious hepatitis virion.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
The complex formed by the binding of antigen and antibody molecules. The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES.
They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system.
The sum of the weight of all the atoms in a molecule.
Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.
A group of the D-related HLA antigens found to differ from the DR antigens in genetic locus and therefore inheritance. These antigens are polymorphic glycoproteins comprising alpha and beta chains and are found on lymphoid and other cells, often associated with certain diseases.
Immunoglobulins produced in response to VIRAL ANTIGENS.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
A glycolipid, cross-species antigen that induces production of antisheep hemolysin. It is present on the tissue cells of many species but absent in humans. It is found in many infectious agents.
Elements of limited time intervals, contributing to particular results or situations.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
An inhibitory B7 antigen that has specificity for the T-CELL receptor PROGRAMMED CELL DEATH 1 PROTEIN. CD274 antigen provides negative signals that control and inhibit T-cell responses and is found at higher than normal levels on tumor cells, suggesting its potential role in TUMOR IMMUNE EVASION.
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
A species of POLYOMAVIRUS originally isolated from Rhesus monkey kidney tissue. It produces malignancy in human and newborn hamster kidney cell cultures.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
Substances that augment, stimulate, activate, potentiate, or modulate the immune response at either the cellular or humoral level. The classical agents (Freund's adjuvant, BCG, Corynebacterium parvum, et al.) contain bacterial antigens. Some are endogenous (e.g., histamine, interferon, transfer factor, tuftsin, interleukin-1). Their mode of action is either non-specific, resulting in increased immune responsiveness to a wide variety of antigens, or antigen-specific, i.e., affecting a restricted type of immune response to a narrow group of antigens. The therapeutic efficacy of many biological response modifiers is related to their antigen-specific immunoadjuvanticity.
Antigens that exist in alternative (allelic) forms in a single species. When an isoantigen is encountered by species members who lack it, an immune response is induced. Typical isoantigens are the BLOOD GROUP ANTIGENS.
Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell.
A melanosome-associated protein that plays a role in the maturation of the MELANOSOME.
The genetic region which contains the loci of genes which determine the structure of the serologically defined (SD) and lymphocyte-defined (LD) TRANSPLANTATION ANTIGENS, genes which control the structure of the IMMUNE RESPONSE-ASSOCIATED ANTIGENS, HUMAN; the IMMUNE RESPONSE GENES which control the ability of an animal to respond immunologically to antigenic stimuli, and genes which determine the structure and/or level of the first four components of complement.
Bone marrow-derived lymphocytes that possess cytotoxic properties, classically directed against transformed and virus-infected cells. Unlike T CELLS; and B CELLS; NK CELLS are not antigen specific. The cytotoxicity of natural killer cells is determined by the collective signaling of an array of inhibitory and stimulatory CELL SURFACE RECEPTORS. A subset of T-LYMPHOCYTES referred to as NATURAL KILLER T CELLS shares some of the properties of this cell type.
A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
Form of passive immunization where previously sensitized immunologic agents (cells or serum) are transferred to non-immune recipients. When transfer of cells is used as a therapy for the treatment of neoplasms, it is called adoptive immunotherapy (IMMUNOTHERAPY, ADOPTIVE).
Immunoglobulins produced in a response to PROTOZOAN ANTIGENS.
Antibodies that react with self-antigens (AUTOANTIGENS) of the organism that produced them.

Kallidin- and bradykinin-degrading pathways in human heart: degradation of kallidin by aminopeptidase M-like activity and bradykinin by neutral endopeptidase. (1/485)

BACKGROUND: Since kinins kallidin (KD) and bradykinin (BK) appear to have cardioprotective effects ranging from improved hemodynamics to antiproliferative effects, inhibition of kinin-degrading enzymes should potentiate such effects. Indeed, it is believed that this mechanism is partly responsible for the beneficial effects of angiotensin-converting enzyme (ACE) inhibitors. In the heart, enzymes other than ACE may contribute to local degradation of kinins. The purpose of this study was to investigate which enzymes are responsible for the degradation of KD and BK in human heart tissue. METHODS AND RESULTS: Cardiac membranes were prepared from the left ventricles of normal (n=5) and failing (n=10) hearts. The patients had end-stage congestive heart failure as the result of coronary heart disease or idiopathic dilated cardiomyopathy. Heart tissue was incubated with KD or BK in the presence or absence of enzyme inhibitors. We found no difference in the enzymes responsible for kinin metabolism or their activities between normal and failing hearts. Thus KD was mostly converted into BK by the aminopeptidase M-like activity. When BK was used as substrate, it was converted into an inactive metabolite BK-(1-7) mostly (80% to 90%) by the neutral endopeptidase (NEP) activity, with ACE unexpectedly playing only a minor role. The low enzymatic activity of ACE in the cardiac membranes, compared with that of NEP, was not due to chronic ACE inhibitor therapy, because the cardiac ACE activities of patients, whether receiving ACE inhibitors or not, and of normal subjects were all equal. CONCLUSIONS: The present in vitro study shows that in human cardiac membranes, the most critical step in kinin metabolism, that is, inactivation of BK, appears to be mediated mostly by NEP. This observation suggests a role for NEP in the local control of BK concentration in heart tissue. Thus inhibition of cardiac NEP activity could be cardioprotective by elevating the local concentration of BK in the heart.  (+info)

Immunophenotype of myeloid cells in myelodysplastic syndromes and its clinical implications. (2/485)

OBJECTIVE: To explore the immunophenotype of myeloid cells in myelodysplastic syndyomes (MDS) and its clinical implications. METHODS: A panel of monoclonal antibody was used to detect CD13+, CD33+, CD15+ and CD14+ antigens on the membrane surfaces of myeloid cells in the bone marrow from 51 MDS, 21 aplastic anemia (AA), 21 paroxysmal nocturnal hemoglobinuria (PNH) patients. 10 acute myeloblastic leukemia (AML) patients and 15 normal subjects by immunoenzymatic assay. The morphology and chromosome karyotype of bone marrow cells of MDS patients were also examined. RESULTS: CD14+, CD13+ and CD33+ cells in the bone marrow were more in MDS patients than in normal controls, AA patients and PNH patients. CD15+ cells in the bone marrow were less in MDS patients than in normal controls. CONCLUSIONS: The percentages of CD14+, CD13+ and CD33+ positive cells in the bone marrow of MDS patients were related to the percentage of myeloblasts, the chromosomal aberrations and the response to treatment. It indicated that there is immunophenotypic misexpression of myeloid cells in MDS patients. Immunophenotype analysis of myeloid cells might be useful for the diagnosis and treatment of MDS patients.  (+info)

The flow cytometric pattern of CD34, CD15 and CD13 expression in acute myeloblastic leukemia is highly characteristic of the presence of PML-RARalpha gene rearrangements. (3/485)

BACKGROUND AND OBJECTIVE: Rapid identification of AML patients carrying the t(15;17) translocation for treatment decision-making is currently made on the basis of morphologic screening. However, the existence of both false positives and negatives highlights the need for more objective methods of screening AML cases and further molecular confirmation of the t(15;17) translocation. DESIGN AND METHODS: In the present study we analyzed a total of 111 AML cases in order to investigate whether immunophenotyping based on the assessment of multiple-stainings analyzed at flow cytometry could improve the sensitivity and specificity of morphologic identification of acute promyelocytic leukemia (APL) carrying the t(15;17) translocation. FISH analysis was used as a complementary technique for cases in which morphology and molecular biology yielded discrepant results. RESULTS: Concordant results between morphology and RT-PCR were found in 102/111 (91.8%) cases: 34 patients had M3/PML-RARalpha+ and 68 non-M3/PML-RARalpha- disease. Nine cases showed discrepants results. Multivariate analysis showed that the best combination of immunologic markers for discriminating between M3/PML-RARalpha+ and non-M3/PML-RARalpha- cases was that of the presence of heterogeneous expression of CD13, the existence of a single major blast cell population, and a characteristic CD34/CD15 phenotypic pattern (p<0.02). A score system based on these parameters was designed, and the 34 M3/PML-RARalpha+ cases showed a score of 3 (presence of the 3 phenotypic characteristics). In contrast, only 1 out of the 68 (1.3%) non-M3/PML-RARalpha- cases had this score, most o these latter cases (53/68, 78%) scoring either 0 or 1. Therefore, among these cases, immunophenotyping showed a sensitivity of 100% and a specificity of 99% for predicting PML/RARalpha gene rearrangements. Of the 9 cases in which morphology and molecular biology results were discrepant, four cases displayed M3 morphology without PML/RARalpha rearrangements by RT-PCR. In only one of these 4 cases did the immunophenotype score 3, this being the only FISH positive case. From the remaining five discrepant cases (non-M3 morphology while positive for PML/RARalpha) two cases had a phenotypic score of 3 and were FISH positive while the other three were negative by FISH. Upon repeating RT-PCR studies, two of these latter three cases became negative. INTERPRETATION AND CONCLUSIONS: Our results show that immunophenotyping may be of great value for quick screening of APL with PML/RARalpha rearrangements.  (+info)

Dissociation between growth arrest and differentiation in Caco-2 subclone expressing high levels of sucrase. (4/485)

Growth arrest and cell differentiation are generally considered temporally and functionally linked phenomena in small intestinal crypt cells and colon tumor cell lines (Caco-2, HT-29). We have derived a Caco-2 subclone (NGI3) that deviates from such a paradigm. In striking contrast with the parental cells, proliferative and subconfluent NGI3 cells were found to express sucrase-isomaltase (SI) mRNA and to synthesize relatively high levels of SI, dipeptidyl peptidase IV, and aminopeptidase N (APN). In postconfluent cells, little difference was seen in SI mRNA levels between Caco-2 and NGI3 cells, but the latter still expressed much higher levels of SI that could be attributed to higher rates of translation. APN expression was also greatly enhanced in NGI3 cells. To determine whether high levels of brush-border enzymes correlated with expression of cell-cycle regulatory proteins, we investigated their relative cellular levels in growing and growth-arrested cells. The results showed that the cyclin-dependent kinase inhibitors (p21 and p27) and D-type cyclins (D1 and D3) were all induced in postconfluent cells, but NGI3 cells expressed much higher levels of p21. This study demonstrated that cell growth and expression of differentiated traits are not mutually exclusive in intestinal epithelial cells and provided evidence indicating that posttranscriptional events play an important role in regulation of SI expression.  (+info)

Temporal association of the N- and O-linked glycosylation events and their implication in the polarized sorting of intestinal brush border sucrase-isomaltase, aminopeptidase N, and dipeptidyl peptidase IV. (5/485)

The temporal association between O-glycosylation and processing of N-linked glycans in the Golgi apparatus as well as the implication of these events in the polarized sorting of three brush border proteins has been the subject of the current investigation. O-Glycosylation of pro-sucrase-isomaltase (pro-SI), aminopeptidase N (ApN), and dipeptidyl peptidase IV (DPPIV) is drastically reduced when processing of the mannose-rich N-linked glycans is blocked by deoxymannojirimycin, an inhibitor of the Golgi-located mannosidase I. By contrast, O-glycosylation is not affected in the presence of swainsonine, an inhibitor of Golgi mannosidase II. The results indicate that removal of the outermost mannose residues by mannosidase I from the mannose-rich N-linked glycans is required before O-glycosylation can ensue. On the other hand, subsequent mannose residues in the core chain impose no sterical constraints on the progression of O-glycosylation. Reduction or modification of N- and O-glycosylation do not affect the transport of pro-SI, ApN, or DPPIV to the cell surface per se. However, the polarized sorting of two of these proteins, pro-SI and DPPIV, to the apical membrane is substantially altered when O-glycans are not completely processed, while the sorting of ApN is not affected. The processing of N-linked glycans, on the other hand, has no influence on sorting of all three proteins. The results indicate that O-linked carbohydrates are at least a part of the sorting mechanism of pro-SI and DPPIV. The sorting of ApN implicates neither O-linked nor N-linked glycans and is driven most likely by carbohydrate-independent mechanisms.  (+info)

Purification, characterization, gene cloning, sequencing, and overexpression of aminopeptidase N from Streptococcus thermophilus A. (6/485)

The general aminopeptidase PepN from Streptococcus thermophilus A was purified to protein homogeneity by hydroxyapatite, anion-exchange, and gel filtration chromatographies. The PepN enzyme was estimated to be a monomer of 95 kDa, with maximal activity on N-Lys-7-amino-4-methylcoumarin at pH 7 and 37 degrees C. It was strongly inhibited by metal chelating agents, suggesting that it is a metallopeptidase. The activity was greatly restored by the bivalent cations Co2+, Zn2+, and Mn2+. Except for proline, glycine, and acidic amino acid residues, PepN has a broad specificity on the N-terminal amino acid of small peptides, but no significant endopeptidase activity has been detected. The N-terminal and short internal amino acid sequences of purified PepN were determined. By using synthetic primers and a battery of PCR techniques, the pepN gene was amplified, subcloned, and further sequenced, revealing an open reading frame of 2,541 nucleotides encoding a protein of 847 amino acids with a molecular weight of 96,252. Amino acid sequence analysis of the pepN gene translation product shows high homology with other PepN enzymes from lactic acid bacteria and exhibits the signature sequence of the zinc metallopeptidase family. The pepN gene was cloned in a T7 promoter-based expression plasmid and the 452-fold overproduced PepN enzyme was purified to homogeneity from the periplasmic extract of the host Escherichia coli strain. The overproduced enzyme showed the same catalytic characteristics as the wild-type enzyme.  (+info)

Quantification of conversion and degradation of circulating angiotensin in rats. (7/485)

The aim of the present study was to quantify with a uniform technique the rates of conversion of ANG I to ANG II in the lung and kidney and the degradation of both peptides to biologically inactive products in the pulmonary, renal, and systemic circulation. We infused the peptides intravenously, into the left ventricle, and into the left renal artery of rats and compared their effects on renal blood flow. The measured change in renal blood flow was used as a bioassay parameter to estimate the concentration of circulating ANG II. Mathematical analysis of our data allowed us to calculate conversion and degradation rates. Furthermore, the role of aminopeptidases A (EC 3.4.11.7) and N (EC 3.4.11.2) in the degradation of the peptides in the kidney was investigated by intrarenal infusion of the inhibitor amastatin. Our results show that the conversion rate of ANG I is 75% in the pulmonary and 21% in the renal circulation. Both peptides are degraded by 5% in the pulmonary, by 67% in the systemic, and by 93% in the renal circulation. Amastatin prevented 60% of the renal degradation of the peptides to inactive products, and this effect could be attributed to inhibition of aminopeptidase N. The results indicate that the converting capacity of the kidney is of minor importance for endocrine generation of ANG II but could be useful for the paracrine production.  (+info)

Demonstration that human mast cells arise from a progenitor cell population that is CD34(+), c-kit(+), and expresses aminopeptidase N (CD13). (8/485)

Human mast cells are known to arise from a CD34(+)/c-kit(+) progenitor cell population that also gives rise to neutrophils, eosinophils, basophils, and monocytes. To further characterize cells within the CD34(+)/c-kit(+) population that yield mast cells, this progenitor was additionally sorted for CD13, a myeloid marker known to appear early on rodent mast cells and cultured human mast cells, but not expressed or expressed at low levels on human tissue mast cells; and cultured in recombinant human (rh) stem cell factor (rhSCF), rh interleukin-3 (rhIL-3; first week only), and rhIL-6. Initial sorts revealed that although the majority of cells in culture arose from the CD34(+)/c-kit(+)/CD13(-) cell population, mast cells arose from a CD34(+)/c-kit(+)/CD13(+) progenitor cell that also gave rise to a population of monocytes. Sequential sorting confirmed that CD34(+)/c-kit(+)/CD13(+) cells in CD34(+)/c-kit(+)/CD13(-) sorts gave rise to the few mast cells observed in CD13(-) sorted cells. CD34(+)/c-kit(+)/CD13(+) cells plated as single cells in the presence of various cytokine combinations gave rise to pure mast cell, monocyte, or mixed mast cell/monocyte progeny. Addition of either rh granulocyte-macrophage colony-stimulating factor (rhGM-CSF) or rhIL-5 to the CD34(+)/c-kit(+)/CD13(+) progenitor cell population cultured in rhSCF, rhIL-3, and rhIL-6 did increase the number of total cells cultured and in the case of rhIL-5, did increase total mast cell numbers. Neither rhGM-CSF or rhIL-5 led to additional cell populations, ie, even with the addition of rhGM-CSF or rhIL-5, only mast cells and monocytes grew from CD34(+)/c-kit(+)/CD13(+) cells. Thus, human mast cells and a population of monocytes arise from precursor cells that express CD34, c-kit, and CD13; and within which, are mast cell, monocyte, and mast/monocyte (bipotential) precursors.  (+info)

In this study, we determined serum aminopeptidase N/CD13 concentrations in patients with NSCLC using an aminopeptidase N/CD13-specific mAb (MH8-11), which inhibits cell motility and angiogenesis in vitro (10). Serum aminopeptidase N/CD13 concentrations were significantly elevated in patients with advanced-stage NSCLC. When the serum aminopeptidase N/CD13 values corresponding to the best diagnostic accuracy to separate the healthy controls and the patients with NSCLC were used as the cutoff value, the sensitivity of aminopeptidase N/CD13 was poorer than that of serum carcinoembryonic antigen; this suggests that serum aminopeptidase N/CD13 is not valuable as a diagnostic marker. However, a high serum aminopeptidase N/CD13 was significantly associated with established adverse prognostic factors in NSCLC, such as advanced stage, poor performance status, and poor response to chemotherapy. Serum aminopeptidase N/CD13 levels were also associated with overall survival in univariate analyses and had an ...
HUMAN coronaviruses (HCV) in two serogroups represented by HCV-229E and HCV-OC43 are an important cause of upper respiratory tract infections1. Here we report that human aminopeptidase N, a cell-surface metalloprotease on intestinal, lung and kidney epithelial cells2-5, is a receptor for human coronavirus strain HCV-229E, but not for HCV-OC43. A monoclonal antibody, RBS, blocked HCV-229E virus infection of human lung fibroblasts, immunoprecipitated aminopeptidase N and inhibited its enzymatic activity. HCV-229E-resistant murine fibroblasts became susceptible after transfection with complementary DNA encoding human aminopeptidase N. By contrast, infection of human cells with HCV-OC43 was not inhibited by antibody RBS and expression of aminopeptidase N did not enhance HCV-OC43 replication in mouse cells. A mutant aminopeptidase lacking the catalytic site of the enzyme did not bind HCV-229E or RBS and did not render murine cells susceptible to HCV-229E infection, suggesting that the virus-binding site may
Aminopeptidase N (Myeloid Plasma Membrane Glycoprotein CD13 or Alanyl Aminopeptidase or Aminopeptidase M or Microsomal Aminopeptidase or gp150 or CD13 or ANPEP or EC 3.4.11.2) - Market research report and industry analysis - 11296626
Aminopeptidase N (APN)/CD13 as ubiquitously expressed membrane peptidase exerts important functions in diverse cellular processes, such as proliferation, migration and differentiation. Previously, a role of APN in the invasiveness of melanoma cells has been demonstrated, but the underlying molecular mechanisms controlling APN expression are not understood. The present study demonstrates that lack of APN expression in primary and established melanoma cells was directly associated with a high-grade DNA methylation status of the myeloid APN promoter. Demethylation by 5-aza-2-desoxycytidine not only induced constitutive and cytokine-regulated APN protein expression but also resulted in an increased APN-dependent migration of melanoma cells. Furthermore, its heterogeneous expression was inversely correlated to the expression of melanocytic marker proteins in established as well as in short-term cultured human melanoma cells. Staining of tissue microarrays generated from a large series of melanoma ...
Mouse Monoclonal Anti-Aminopeptidase N/CD13 Antibody (CC81) [DyLight 488]. Validated: Flow. Tested Reactivity: Bovine. 100% Guaranteed.
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual ...
Membrane alanyl aminopeptidase (EC 3.4.11.2) also known as alanyl aminopeptidase (AAP) or aminopeptidase N (AP-N) is an enzyme that in humans is encoded by the ANPEP gene. Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, ...
Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract ...
The IUPHAR/BPS Guide to Pharmacology. Leucyl-cysteinyl aminopeptidase - M1: Aminopeptidase N. Detailed annotation on the structure, function, physiology, pharmacology and clinical relevance of drug targets.
CD13/aminopeptidase N (APN), which is a zinc-dependent metalloproteinase, plays a vital role in the growth, migration, angiogenesis, and metastasis of tumours. Thus, in situ molecular imaging of endogenous APN levels is considerably significant for investigating APN and its different functions. In this study 2018 Chemical Science HOT Article Collection
Studies have come to conflicting conclusions about whether adiponectin (APN) expression is associated with cancer prognosis. To help resolve this question, we meta-analyzed the available evidence. PubMed, EMBASE, the Cochrane Library, the Chinese Biological Medical Database and the Chinese National Knowledge Infrastructure Database were systematically searched to identify all eligible studies examining APN expression and prognosis for patients with any type of cancer. Pooled hazard ratios (HRs) and corresponding 95 % confidence intervals (CIs) related to overall survival (OS) or disease-free survival (DFS) were calculated. Ten studies involving 999 patients were meta-analyzed. Analysis across all patients revealed no significant association between high/positive APN expression and DFS, but they did show a significant association between high/positive APN expression and OS (HR 1.51, 95 %CI 1.21 to 1.89). Subgroup analysis showed that high/positive APN expression in non-Asians was significantly associated
In the human T-cellline H9 a neutral aminopeptidase activity was detected that appeared to be distinguished from other known aminopeptidases with respect to its biochemical characterisation. The aim of this work was to identify this enzyme and to subject it to comprehensive biochemical characterization. The enzyme was purified to homogeneity by the application of a number of conventional chromatographic methods and identified by means of mass spectrometry (Maldi TOF) as cytosol alanyl aminopeptidase (EC 3.4.11.14; cAAP, PSA). The biochemical characteristics of the H9 cell-derived peptidase revealed similarities to the corresponding enzyme of other tissues, however, substantial differences have been found out. Apparently, due to these tissue-specific differences there have been assigned different names to this enzyme before. The cytosolic alanyl aminopeptidase showed high sensitivity to inhibitors that have been regarded as specific for the membrane-bound aminopeptidase N (APN): Phebestin, ...
The invention relates to the use of inhibitors of dipeptidyl peptidase IV DP IV and enzymes having the same specific nature of substrate DP IV enzyme activitycombined with inhibitors of alanyl aminopeptidase aminopeptidase N, APNor enzymes having the same specific nature of substrate APN enzyme Try the new Google Patents, with machine-classified Google Scholar results, and Japanese and South Korean patents.. Combined use of enzyme inhibitors for the treatment and prevention of allergic reactions of type i according to the gell and coombs classification, and for the treatment and prevention of dermatological diseases associated with follicular kostengünstige Sprays bei Psoriasis epidermal hyperkeratosis and reinforced keratinocyte proliferation EP B1. The invention kostengünstige Sprays bei Psoriasis to the use of inhibitors of dipeptidyl peptidase IV DP IV and enzymes having the same specific nature of substrate DP IV enzyme activitycombined with inhibitors of alanyl aminopeptidase ...
Després de la bona acollida assolida amb Verkami del 1r cd de Cançons des del mòbil, ara és el moment de recollir les noves cançons al 2n cd
Anpep - mouse gene knockout kit via CRISPR, 1 kit. |dl||dt|Kit Component:|/dt||dd|- |strong|KN301326G1|/strong|, Anpep gRNA vector 1 in |a href=http://www.origene.com/CRISPR-CAS9/Detail.
Aminopeptidase with broad substrate specificity to several peptides. Shows strong preference for leucine but cleaves also next to Arg and lysine in peptide-bond-containing substrates.
To determine the primary structure of CD13, a 150-kD cell surface glycoprotein originally identified on subsets of normal and malignant human myeloid cells, we isolated the complete sequences encoding the polypeptide in overlapping complementary DNA (cDNA) clones. The authenticity of our cDNA clones …
The graphic displays domains and Protease cut sites on the protein sequence. Drag your mouse right/left over the graphic. Use the selection boxes on the right to select which annotations to view simultaneously. Combine annotation with multiple checkmarks.. ...
Use Bio-Rads PrimePCR assays, controls, templates for your target gene. Every primer pair is optimized, experimentally validated, and performance guaranteed.
Summary The pepN gene has been cloned into the multicopy plasmid pBR322. The restriction map of the insert was established and the gene was localized. By comparison with the restriction map of the plasmid pJP30 bearing the ompF region, it has been possible to order the ompF, asnS, and pepN genes.... mehr ...
In addition to their well characterized effects at dopamine receptors, neuroleptic drugs have been shown to affect the level and in vitro metabolism of neuropeptides. In the present study, the effect of acute and subchronic administration of the neuroleptic haloperidol and the nonselective, dopamine agonist apomorphine on neuropeptidase activity was determined in regional, rat brain P2 membranes. Subchronic administration of haloperidol decreased the activity of aminopeptidase N in the frontal cortex and caudate-putamen. In contrast, subchronic administration of apomorphine increased aminopeptidase N activity in the frontal cortex and caudate-putamen. Neutral endopeptidase 24.11 also was affected differentially in the caudate-putamen, but both subchronic haloperidol and apomorphine decreased neutral endopeptidase 24.11 activity in the frontal cortex. Metalloendopeptidase 24.15 activity was decreased in the caudate-putamen after acute haloperidol and increased in the frontal cortex after acute ...
F4⁺ enterotoxigenic Escherichia coli (ETEC) strains cause diarrheal disease in neonatal and post-weaned piglets. Several different host receptors for F4 fimbriae have been described, with porcine aminopeptidase N (APN) reported most recently. The FaeG subunit is essential for the binding of the three F4 variants to host cells. Here we show in both yeast two-hybrid and pulldown assays that APN bind ...
Zhao M, Yuan X, Wei J, et al. Functional Roles Of Cadherin, Aminopeptidase-N And Alkaline Phosphatase From Helicoverpa Armigera (Hübner) In The Action Mechanism Of Bacillus Thuringiensis Cry2Aa[J]. Scientific Reports, 2017.
Acétylmuramyl Alanyl Isoglutamine; acetylmuramyl alanyl isoglutamine; alanyl isoglutamine, acetylmuramyl; dipeptide, muramyl; isoglutamine, acetylmuramyl alanyl; mur nac l ala d isogln; muramyl dipeptide; mur-nac-l-ala-d-isogln; n acetyl muramyl l alanyl d glutamic alpha amide; n acetylmuramyl l alanyl d isoglutamine; n-acetyl-muramyl-l-alanyl-d-glutamic-alpha-amide; n-acetylmuramyl-l-alanyl-d-isoglutamine. Aide pour diagnostic médical. Méthode automatique dassociations de symptômes pour classifier les plus de 4.000 maladies en quatre langues différentes
The objective of this study was to determine rabbit seminal plasma enzyme activity. Furthermore, correlations between semen parameters and enzyme activity and male age were examined. The study was performed using 17 New Zealand White males from 5 to 9 mo old. Overall, 252 semen samples were collected from bucks from May to September. Semen characteristics were analysed and the seminal plasma was obtained by centrifugation. The activities of alanyl aminopeptidase (APN), aspartate transaminase (AST), alanine aminotransferase (ALT), gamma-glutamyl transpeptidase (GOT), lactate dehydrogenase (LDH) and alkaline phosphatase (ALKP) in the seminal plasma fluid were measured. Significant differences between males were found in APN, GGT, LDH, ALKP and ALT activities (P<0.05). No significant differences between enzyme activity and male age were found. We also observed significant positive correlations between male age and sperm concentration (r=0.26), progressive motility (r=0.17) and amplitude of ...
Melflufen (melphalan flufenamide, previously designated J1) is an anticancer drug under development by Oncopeptides AB. It is a peptidase enhanced cytotoxic (PEnC) that exerts a targeted delivery of melphalan in cells with high expression of aminopeptidases, such as aminopeptidase N, which has been described as over-expressed in human malignancies. Aminopeptidase N plays a functional role in malignant angiogenesis. Currently, melflufen is under evaluation in a multinational and randomized phase III study for the treatment of patients with relapsed and refractory multiple myeloma. Melflufen is metabolized by aminopeptidase hydrolysis and by spontaneous hydrolyisis on N-mustard. Its biological half-life is 10 minutes in vitro. Melflufen is a peptidase enhanced cytotoxic (PEnC) with a targeted delivery within tumor cells of melphalan, a widely used classical chemotherapeutic belonging to a group of alkylating agents developed more than 50 years ago. Substantial clinical experience has been ...
The co-distribution of SFN with APN at the leading edge of fibroblasts was unpredicted. Interestingly, an in vitro wound scratch assay revealed higher expression of APN within the migratory fibroblasts, where it co-distributed with vinculin, a migration marker (Lai et al., 2010). In addition, the inhibition of cell migration by reducing or blocking APN levels in tumor cells, lymphocytes and endothelial cells has also been reported (Terauchi et al., 2007; Proost et al., 2007). Therefore, the higher expression of APN receptor at the leading edge of migratory cells could explain the enhanced co-distribution results observed with biotin-SFN. Furthermore, the movement of cells through ECM is highly dependent on the activity of proteases such as MMPs (Gianneli et al., 1997; Kessenbrock et al., 2010; Pilcher et al., 1997; Pirila et al., 2003). This raises the possibility that SFN interaction with APN and the subsequent increase in MMP-1 activity could play a role in the cell migration of ...
Prices are in US dollars.. These products are for laboratory research purposes only, not for any human or animal diagnostic or therapeutic use.. All site content © 2017 Cell Sciences, Inc.. ...
Prices are in US dollars.. These products are for laboratory research purposes only, not for any human or animal diagnostic or therapeutic use.. All site content © 2017 Cell Sciences, Inc.. ...
CHAPTER LXXVIII. 203 Jh 4^ !j3 .2-^ aS-: 04g what mon fal ames vish ^ 0 w CD -^ a-c i^, w-w Uesckiption of the Single VISHTIS. -c; .£ Ti fO N CD o3 rc; ■^ t+H 0 c3 f,, A cS ^ OJ -C 4J Pi 1-1 It is white, has three eyes, and rides on an elephant, which always remains the same. In the one hand he has a huge rock, in the other a vajra of iron, which it throws. It destroys the cattle over which it rises. He who makes war coming from the direction whence it rises will be victorious. A man must not turn with his face towards it when tearing out fattening herbs, digging out treasures, and trying to satisfy the wants of life. It has the colour of crystal. In one hand it holds a three¬ fold parasvadha, and in the other a rosary. It looks towards heaven, and saj^s hd hd hd. It rides on an ox. Its time is favourable for handing over the chil¬ dren to the schools, for con¬ cluding peace, giving alms, and works of piety. ■Sg2 si osq It is pistachio-coloured like a parrot. It looks like ...
Aerobic activity іѕ аnу activity thаt requires сlеаn air tο produce thе nесеѕѕаrу ATP (adenosine triphosphate) tο carry out thе activity. Aerobic work out activity gets ATP through thе Krebs Design. Once through thе Krebs pattern generates 36 ATPs compared tο јυѕt 2 ATP through glycolysis οr anaerobic pattern. Aerobic work out activity іѕ essential fοr уουr center health.. Aerobic activity achieves thе following:. 1. Increases уουr VO2 max (уουr maximum сlеаn air uptake). It dοеѕ thіѕ bу helping thе quality οf сlеаn air уουr human іѕ аblе tο υѕе during regular aerobic work out activities. If уουre out οf shape аnd rυn a range, уου mіght rυn іt іn ten minutes οr ѕο, bυt іf уου hаνе a regular aerobic workout routine (whісh сουld involve operating, walking, managing, driving, οr аnу center activity), уου саn speed up managing thе range bу minutes bесаυѕе уουll bе breathing more ...
CD13, also known as aminopeptidase N, APN and gp150, is a 150-170 kDa transmembrane ectoenzyme occurring on monocytes and granulocytes but not lymphocytes in blood.
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A story: In the mid-1990s, I participated inside a PSA guideline group for Malignancy Care Ontario. The usual literature search was carried out, which focused on the evidence of mortality benefit and the potential harms of screening. I have been building decision types of PSA testing2 and measuring quality-of-life and price results for a long time. I was somewhat chagrined that non-e of my documents resulted in in the search. I had been created by it question, So why will be the remaining committee and We viewing this nagging issue thus differently? My model recommended that there could be a mortality advantage but general a lack of quality-adjusted life span. This meant that the true manner in which patients valued health outcomes was an integral area of the testing decision. Quite simply, this is a preference-sensitive decision. Furthermore, it seemed as well obvious to say that price was another concern Mouse monoclonal to CD13.COB10 reacts with CD13, 150 kDa aminopeptidase N (APN). CD13 is ...
Opiorfin je endogeno hemijsko jedinjenje koje je prvo izolovano iz ljudske pljuvačke. Inicijalna istraživanja na miševima su pokazala da ovo jedinjenje ima veći analgetski efekat od morfina. Opiorfin deluje putem zaustavljanja normalnog razlaganja prirodnih analgetskih opioida u kičmenoj moždini, zvanih enkefalini. On je relativno mali peptid koji se sastoji od pet aminokiselina: Gln-Arg-Phe-Ser-Arg.[1][2][3][4][5][6][7][8] Opiorfin pentapetid potiče od N-terminalnog regiona proteina PROL1 (prolinom bogat, lakrimal 1).[1] Opiorfin inhibira dve proteaze: neutralnu ekto-endopeptidazu (MME) i ekto-aminopeptidazu N (ANPEP).[1] ...
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3-Fluoro-DL-tyrosine can be used to study how halogenated tyrosines affect proteins such as bacteriorhodopsin, microvillar enzymes, and β-galactosidases.
The pig intestinal brush border enzymes aminopeptidase N (EC 3.4.11.2) and lactase-phlorizin hydrolase (EC 3.2.1.23-62) are present in the microvillar membrane as homodimers. Dimethyl adipimidate was used to cross-link the two [35S]methionine-labeled brush border enzymes from cultured mucosal explants. For aminopeptidase N, dimerization did not begin until 5-10 min after synthesis, and maximal dimerization by cross-linking of the transient form of the enzyme required 1 h, whereas the mature form of aminopeptidase N cross-linked with unchanged efficiency from 45 min to 3 h of labeling. Formation of dimers of this enzyme therefore occurs prior to the Golgi-associated processing, and the slow rate of dimerization may be the rate-limiting step in the transport from the endoplasmic reticulum to the Golgi complex. For lactase-phlorizin hydrolase, the posttranslational processing includes a proteolytic cleavage of its high molecular weight precursor. Since only the mature form and not the precursor of this
Porcine deltacoronavirus (PDCoV), can be an emerging enteropathogenic coronavirus in pigs, that poses a novel threat to swine husbandry worldwide. contamination (Godet et al., 1994) and in severe acute respiratory syndrome coronavirus (SARS-CoV) in the genus (Li et al., 2005). In contrast, the RBD regions of murine hepatitis computer virus and bovine coronavirus, both in the genus are located in the N-terminus of the S1 domain name (Peng et al., 2011, 2012). However, the immunodominant neutralizing region associated with delta-CoVs, such as PDCoV, has not been identified. Recent elucidation of PDCoV spike protein structures by cryo-electron microscopy reveal that this S1 subunit consists of four independently folded domains, specified A, B, C, and D (Xiong et al., 2018). Lately, Li et al. (Li et al., 2018) confirmed the fact that porcine aminopeptidase N, previously regarded as an operating receptor for transmissible gastroenteritis pathogen (TGEV), also interacts using the B area of PDCoV S1 ...
The property of solutions of Triton X-114 to separate into detergent-rich and detergent-poor phases at 30 degrees C has been exploited to investigate the identities of the aminopeptidases in synaptic membrane preparations from pig striatum. When titrated with an antiserum to aminopeptidase N (EC 3.4.11.2), synaptic membranes solubilized with Triton X-100 revealed that this enzyme apparently comprises no more than 5% of the activity releasing tyrosine from [Leu]enkephalin. When assayed in the presence of puromycin, this proportion increased to 20%. Three integral membrane proteins were fractionated by phase separation in Triton X-114. Aminopeptidase activity, endopeptidase-24.11 and peptidyl dipeptidase A partitioned predominantly into the detergent-rich phase when kidney microvillar membranes were so treated. However, only 5.5% of synaptic membrane aminopeptidase activity partitioned into this phase, although the other peptidases behaved predictably. About half of the aminopeptidase activity in ...
To analyze the influence of cell differentiation and the effects of hormones on the subcellular distribution of apical antigens in polarized epithelial cells, we have compared the localization of three brush border (BB) hydrolases [neutral endopeptidase (ENDO), aminopeptidase N (APN), and dipeptidyl …
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The E-Lib is a centralized database of information on APN projects and related outputs. It contains metadata and outputs of all completed and ongoing APN projects since 1998, as well as APN publications such as the Science Bulletin, books, proceedings, reports and policy briefs, etc.
If you are interested in phonographs, gramophones and the history of recorded sound, then the Canadian Antique Phonograph Society (CAPS) is for you. This site provides a glimpse of the Societys programs and activities, and highlights from its bi-monthly publication Antique Phonograph News (APN).
The present invention relates to isolated polypeptides having aminopeptidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
cytosol, aminopeptidase activity, identical protein binding, manganese ion binding, metalloexopeptidase activity, peptidase activity, protein homotetramerization
The protein encoded by this gene is an aminopeptidase which prefers acidic amino acids, and specifically favors aspartic acid over glutamic acid. It is thought to be a cytosolic protein involved in general metabolism of intracellular proteins. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016 ...
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Analysis of Antimalarial Synergy between Bestatin and Endoprotease Inhibitors Using Statistical Response-Surface Modelling: The pathway of hemoglobin degradatio
Pelatihan PONEK - Pelatihan FKTP - Pelatihan MFK - Pelatihan PPGDON - Pelatihan APN - Pelatihan IPCN - Pelatihan Geriatri - Pelatihan Asesor - Pelatihan Case Manajer - Pelatihan Clinical Pathway. Pelatihan PONEK 2020, Pelatihan PONED 2020, Pelatihan Keperawatan 2020, Pelatihan Asesor Perawat, Pelatihan Asesor Bidan, Pelatihan Geriatri 2020, Pelatihan PPI 2020, Pelatihan IPCD 2020, Pelatihan MFK, Pelatihan Asesor Keperawatan, Pelatihan Asesor Bidan 2020, Pelatihan Geriatri, Pelatihan PPI, Pelatihan IPCD, Pelatihan IPCN, Pelatihan Humas Dan Marekting Rumah Sakit, Pelatihan ANJAB, Pelatihan PMKP, Pelatihan HIV Dan AIDS, Pelatihan FKTP, Pelatihan EWS, Pelatihan IPAL, Pelatihan, Pelatihan Kredensial Rumah Sakit, Pelatihan Case Manager, Pelatihan Clinical Pathway, Pelatihan Arsip Elektronik Rumah Sakit, Pelatihan Arsip Elektronik Pemda, Pelatihan Gerontik, Pelatihan APN, Pelatihan HDP, Pelatihan K3 Rumah Sakit, Pelatihan Koding, Pelatihan Gizi Rumah Sakit, Pelatihan Kepala Bidang Keperawatan, ...
Pelatihan PONEK - Pelatihan FKTP - Pelatihan MFK - Pelatihan PPGDON - Pelatihan APN - Pelatihan IPCN - Pelatihan Geriatri - Pelatihan Asesor - Pelatihan Case Manajer - Pelatihan Clinical Pathway. Pelatihan PONEK 2020, Pelatihan PONED 2020, Pelatihan Keperawatan 2020, Pelatihan Asesor Perawat, Pelatihan Asesor Bidan, Pelatihan Geriatri 2020, Pelatihan PPI 2020, Pelatihan IPCD 2020, Pelatihan MFK, Pelatihan Asesor Keperawatan, Pelatihan Asesor Bidan 2020, Pelatihan Geriatri, Pelatihan PPI, Pelatihan IPCD, Pelatihan IPCN, Pelatihan Humas Dan Marekting Rumah Sakit, Pelatihan ANJAB, Pelatihan PMKP, Pelatihan HIV Dan AIDS, Pelatihan FKTP, Pelatihan EWS, Pelatihan IPAL, Pelatihan, Pelatihan Kredensial Rumah Sakit, Pelatihan Case Manager, Pelatihan Clinical Pathway, Pelatihan Arsip Elektronik Rumah Sakit, Pelatihan Arsip Elektronik Pemda, Pelatihan Gerontik, Pelatihan APN, Pelatihan HDP, Pelatihan K3 Rumah Sakit, Pelatihan Koding, Pelatihan Gizi Rumah Sakit, Pelatihan Kepala Bidang Keperawatan, ...
Unlike myeloid dendritic cells, myeloid antigens like CD11b, CD11c, CD13, CD14 and CD33 are not present on pDC surfaces. ... In humans, pDCs exhibit plasma cell morphology and express CD4, HLA-DR, CD123, blood-derived dendritic cell antigen-2 (BDCA-2 ... Villadangos, José A.; Young, Louise (September 2008). "Antigen-Presentation Properties of Plasmacytoid Dendritic Cells". ... which allows the cell to optimize its antigen-presenting abilities. MHC class I on pDC surfaces are able to activate CD8+ T ...
... close correlation with expression of myeloid-associated antigen CD13". Leukemia. 6 (7): 662-8. PMID 1378163. Lynch M, Baker E, ...
The blasts react with antibodies to myeloperoxidase and antibodies to CD13, CD33, and CD34. Human leukocyte antigen (HLA)-DR is ... but immunophenotyping demonstrates myeloid antigens. In acute myeloblastic leukemia (M0), the blasts are agranular and ...
The MEROPS online database for peptidases and their inhibitors: M01.001 CD13+Antigens at the US National Library of Medicine ... Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme ... Look AT, Ashmun RA, Shapiro LH, Peiper SC (April 1989). "Human myeloid plasma membrane glycoprotein CD13 (gp150) is identical ... Söderberg C, Giugni TD, Zaia JA, Larsson S, Wahlberg JM, Möller E (November 1993). "CD13 (human aminopeptidase N) mediates ...
... antigens, cd13 MeSH D08.811.277.656.350.555.200 - carboxypeptidase b MeSH D08.811.277.656.350.555.250 - carboxypeptidase h MeSH ... antigens, cd13 MeSH D08.811.277.656.675.555.200 - carboxypeptidase b MeSH D08.811.277.656.675.555.250 - carboxypeptidase h MeSH ... antigens, cd13 MeSH D08.811.277.656.350.100.235 - cystinyl aminopeptidase MeSH D08.811.277.656.350.100.373 - glutamyl ... antigens, cd38 MeSH D08.811.277.450.770 - oligo-1,6-glucosidase MeSH D08.811.277.450.770.800 - sucrase-isomaltase complex MeSH ...
... antigens, cd7 MeSH D23.050.301.264.894.108 - antigens, cd8 MeSH D23.050.301.264.894.113 - antigens, cd13 MeSH D23.050.301.264. ... antigens, cd13 MeSH D23.050.301.264.035.114 - antigens, cd14 MeSH D23.050.301.264.035.115 - antigens, cd15 MeSH D23.050.301.264 ... antigens, cd13 MeSH D23.101.100.110.114 - antigens, cd14 MeSH D23.101.100.110.115 - antigens, cd15 MeSH D23.101.100.110.118 - ... antigens, cd7 MeSH D23.101.100.894.108 - antigens, cd8 MeSH D23.101.100.894.113 - antigens, cd13 MeSH D23.101.100.894.118 - ...
This antigen along with other blood group antigens was used to identify the Basque people as a genetically separate group.[49] ... Because the Duffy antigen is uncommon in those of Black African descent, the presence of this antigen has been used to detect ... The Fy4 antigen, originally described on Fy (a-b-) RBCs, is now thought to be a distinct, unrelated antigen and is no longer ... The Duffy antigen is expressed in greater quantities on reticulocytes than on mature erythrocytes.[21] While the Duffy antigen ...
Rehli M, Krause SW, Kreutz M, Andreesen R (June 1995). "Carboxypeptidase M is identical to the MAX.1 antigen and its expression ... "Distribution of carboxypeptidase M on lymphoid and myeloid cells parallels the other zinc-dependent proteases CD10 and CD13". ...
White Cell Differentiation Antigens. Oxford University Press. Knapp, W; et al. (1989). Leucocyte Typing IV. Oxford University ... Antibodies CD71/Transferrin receptor-1 CD13 marker for kidney disorder CD33 marker for AML treatment CD4+/CD8+ ratio Immune ... "CD Antigens" (PDF). abcam. 2009. Retrieved 2014-11-22. Passlick B, Flieger D, Ziegler-Heitbrock HW (1989). "Identification and ... In the example of CD4 & CD8, these molecules are critical in antigen recognition. Others (e.g., CD135) act as cell surface ...
... +Antigen at the US National Library of Medicine Medical Subject Headings (MeSH) Human CD164 genome location and CD164 gene ... 2005). "Identification of CD13, CD107a, and CD164 as novel basophil-activation markers and dissection of two response patterns ...
Azuma Y, Ito M, Taniguchi A, Matsumoto K (June 2004). "Expression of cell surface Lewis X and Y antigens and FUT4 mRNA is ... Cruse JM, Lewis RE, Sanders CM, Webb RN, Beason KL, Lam J, Koehler J (October 2007). "Diminished CD10, CD13, and CD15 ... Azuma Y, Kurusu Y, Sato H, Higai K, Matsumoto K (April 2007). "Increased expression of Lewis X and Y antigens on the cell ... Higai K, Ichikawa A, Matsumoto K (September 2006). "Binding of sialyl Lewis X antigen to lectin-like receptors on NK cells ...
... and sialyl Tn antigens in colorectal cancer patients: multivariate analysis of predictive factors for serum antigen levels". ... "A functional glycoproteomics approach identifies CD13 as a novel E-selectin ligand in breast cancer". Biochimica et Biophysica ... which is a glycoprotein that serves as a ligand for macrophage-1 antigen (Mac-1) and lymphocyte function-associated antigen 1 ( ... which is a glycoprotein that serves as a ligand for macrophage-1 antigen (Mac-1) and lymphocyte function-associated antigen 1 ( ...
doi:10.1158/2159-8290.CD-13-0424. PMC 4154497. PMID 24771846. Robinson G, Parker M, Kranenburg TA, Lu C, Chen X, Ding L, et al ... "An N-acetylated natural ligand of human histocompatibility leukocyte antigen (HLA)-B39. Classical major histocompatibility ...
"Direct association of adenosine deaminase with a T cell activation antigen, CD26". Science. 261 (5120): 466-9. doi:10.1126/ ...
In addition to aiding with cytotoxic T cell antigen interactions the CD8 co-receptor also plays a role in T cell signaling. The ... the CD8 co-receptor plays a role in T cell signaling and aiding with cytotoxic T cell antigen interactions. ... This affinity keeps the T cell receptor of the cytotoxic T cell and the target cell bound closely together during antigen- ... Once the T cell receptor binds its specific antigen Lck phosphorylates the cytoplasmic CD3 and ζ-chains of the TCR complex ...
... is a co-receptor of the T cell receptor (TCR) and assists the latter in communicating with antigen-presenting cells. The ... Leucocyte typing: human leucocyte differentiation antigens detected by monoclonal antibodies: specification, classification, ... T cells displaying CD4 molecules (and not CD8) on their surface, therefore, are specific for antigens presented by MHC II and ... CD1+Antigen at the US National Library of Medicine Medical Subject Headings (MeSH) ...
CD64+Antigens at the US National Library of Medicine Medical Subject Headings (MeSH) ...
It is also called Lewis x and SSEA-1 (stage-specific embryonic antigen 1) and represents a marker for murine pluripotent stem ... CD15 Antigen at the US National Library of Medicine Medical Subject Headings (MeSH) ... CD15 (3-fucosyl-N-acetyl-lactosamine) is a cluster of differentiation antigen - an immunologically significant molecule. CD15 ...
Mast cell differentiation antigens: expression in normal and malignant cells and use for diagnostic purposes". Eur. J. Clin. ... The leukemic cells usually are strongly positive for CD13, CD33, CD68, and CD117. Characteristically, basophil (e.g. CD11b, ...
Recently, Heneberg proposed that basophils may be defined as the cellular population positive for CD13, CD44, CD54, CD63, CD69 ... pollen proteins or helminth antigens. Recent studies in mice suggest that basophils may also regulate the behavior of T cells ... When activated, some additional surface markers are known to be upregulated (CD13, CD107a, CD164), or surface-exposed (CD63, ...
Examples of tumoral markers used to follow up cancer treatment are the Carcinoembryonic Antigen (CEA) for colorectal cancer and ... doi:10.1158/2159-8290.CD-13-1014. ISSN 2159-8274. PMC 4433544. PMID 24801577.. ... and can discriminate between a large sample of cells based on their antigens. An example of a cellular biomarker sorting ... the Prostate Specific Antigen (PSA) for prostate cancer. In 2014, Cancer research identified Circulating Tumor Cells (CTCs) and ...
1997). "The Oka blood group antigen is a marker for the M6 leukocyte activation antigen, the human homolog of OX-47 antigen, ... 1992). "Human leukocyte activation antigen M6, a member of the Ig superfamily, is the species homologue of rat OX-47, mouse ... Kasinrerk W, Fiebiger E, Stefanová I, Baumruker T, Knapp W, Stockinger H (1992). "Human leukocyte activation antigen M6, a ... Ok blood group system at BGMUT Blood Group Antigen Gene Mutation Database at NCBI, NIH ...
Tissue Antigens (англ.)русск. : journal. - 2007. - Vol. 68, no. 6. - P. 509-517. - DOI:10.1111/j.1399-0039.2006.00726.x. - PMID ...
CD74 (англ. HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain) - мембранный белок ... II histocompatibility antigen gamma chaingamma chain of class II antigensIiHLA-DR antigens-associated invariant chainIa antigen ... Riberdy J.M., Newcomb J.R., Surman M.J., Barbosa J.A., Cresswell P. HLA-DR molecules from an antigen-processing mutant cell ... Machamer C.E., Cresswell P. Biosynthesis and glycosylation of the invariant chain associated with HLA-DR antigens (англ.) // ...
In humans, the CD44 antigen is encoded by the CD44 gene on Chromosome 11.[5] CD44 has been referred to as HCAM (homing cell ... The CD44 antigen is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. ... Indian blood group system at BGMUT Blood Group Antigen Gene Mutation Database at NCBI, NIH ... "Carcinoembryonic antigen and CD44 variant isoforms cooperate to mediate colon carcinoma cell adhesion to E- and L-selectin in ...
... uveitis antigens induce CXCR3- and CXCR5-expressing lymphocytes and immature dendritic cells to migrate (англ.) // Blood (англ ...
CD1 (a-c, 1A, 1D, 1E) • CD2 • CD3 (γ, δ, ε) • CD4 • CD5 • CD6 • CD7 • CD8 (a) • CD9 • CD10 • CD11 (a, b, c) • CD13 • CD14 • ... CD97 antigen je protein koji je kod ljudi kodiran CD97 genom.[1][2][3] ... 2001). „Tissue distribution of the human CD97 EGF-TM7 receptor". Tissue Antigens. 57 (4): 325-31. PMID 11380941. doi:10.1034/j. ... Expression cloning and chromosomal mapping of the leukocyte activation antigen CD97, a new seven-span transmembrane molecule of ...
Seligman P. A., Butler C. D., Massey E. J., etal. The p97 antigen is mapped to the q24-qter region of chromosome 3; the same ... Le Beau M. M., Diaz M. O., Plowman G. D., etal. Chromosomal sublocalization of the human p97 melanoma antigen. (англ.) // Hum. ... Plowman G. D., Brown J. P., Enns C. A., etal. Assignment of the gene for human melanoma-associated antigen p97 to chromosome 3 ... Rose T. M., Plowman G. D., Teplow D. B., etal. Primary structure of the human melanoma-associated antigen p97 ( ...
Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) also known as CD66e (Cluster of Differentiation 66e), is a ... 2001). "Heterogeneous RNA-binding protein M4 is a receptor for carcinoembryonic antigen in Kupffer cells". J. Biol. Chem. 276 ( ... CEACAM5, CD66e, CEA, carcinoembryonic antigen related cell adhesion molecule 5. External IDs. HomoloGene: 128801 GeneCards: ... Oikawa S, Nakazato H, Kosaki G (1987). "Primary structure of human carcinoembryonic antigen (CEA) deduced from cDNA sequence". ...
When it is functional, it can cause immune unresponsiveness to self-antigens via both central and peripheral tolerance. In the ... doi:10.1158/2159-8290.CD-13-0609. PMC 3975047. PMID 24346116. Liao G (12 August 2011). "ABT-199 BH-3 Mimetic Enters Phase Ia ... Due to the fact that dendritic cells are the immune system's most important antigen-presenting cells, their activity must be ... Antibodies to Bcl-2 can be used with immunohistochemistry to identify cells containing the antigen. In healthy tissue, these ...
CD1 (a-c, 1A, 1D, 1E) • CD2 • CD3 (γ, δ, ε) • CD4 • CD5 • CD6 • CD7 • CD8 (a) • CD9 • CD10 • CD11 (a, b, c) • CD13 • CD14 • ... 2000). "Characterization of a new member of the TNF family expressed on antigen presenting cells.". Biol. Chem. 380 (12): 1443- ... "BLyS receptor signatures resolve homeostatically independent compartments among naïve and antigen-experienced B cells.". Semin ...
I. Partial characterization of soluble Ki-1 antigen and detection of the antigen in cell culture supernatants and in serum by ... Josimovic-Alasevic O, Dürkop H, Schwarting R, Backé E, Stein H, Diamantstein T (Jan 1989). "Ki-1 (CD30) antigen is released by ... CD30+Antigens at the US National Library of Medicine Medical Subject Headings (MeSH) ... results from cDNA cloning and sequence comparison of the CD30 antigen from different sources". Molecular Immunology. 31 (17): ...
Macrophage-1 antigen (CD11b+CD18). *VLA-4 (CD49d+CD29). *Glycoprotein IIb/IIIa (ITGA2B+ITGB3) ...
The protein also carries the Jr(a) antigen, which defines the Junior blood group system.[9] ...
van Rhenen A., van Dongen G. A., Kelder A., et al. The novel AML stem cell associated antigen CLL-1 aids in discrimination ...
"Interaction of glycogen synthase kinase 3beta with the DF3/MUC1 carcinoma-associated antigen and beta-catenin". Molecular and ...
Ebert LM, McColl SR (2002). "Up-regulation of CCR5 and CCR6 on distinct subpopulations of antigen-activated CD4+ T lymphocytes ... This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may ... dendritic cells induce antitumor immunity when genetically fused with nonimmunogenic tumor antigens". J. Immunol. 167 (11): ...
A new ligand for human leukocyte antigen class II antigens". The Journal of Experimental Medicine. 176 (2): 327-37. doi:10.1084 ... A new ligand for human leukocyte antigen class II antigens". The Journal of Experimental Medicine. 176 (2): 327-37. doi:10.1084 ... antigen processing and presentation of exogenous peptide antigen via MHC class II. ... antigen binding. • transmembrane signaling receptor activity. • MHC class II protein binding. Cellular component. • membrane. • ...
CD1 (a-c, 1A, 1D, 1E) • CD2 • CD3 (γ, δ, ε) • CD4 • CD5 • CD6 • CD7 • CD8 (a) • CD9 • CD10 • CD11 (a, b, c) • CD13 • CD14 • ... CD97 antigen je protein koji je kod ljudi kodiran CD97 genom.[1][2][3] ... 2001). "Tissue distribution of the human CD97 EGF-TM7 receptor". Tissue Antigens 57 (4): 325-31. PMID 11380941. doi:10.1034/j. ... "Expression cloning and chromosomal mapping of the leukocyte activation antigen CD97, a new seven-span transmembrane molecule of ...
"Entrez Gene: ITGB3 integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)".. *^ May, K. E.; Villar, J.; Kirtley, S.; ... CD61+Antigens at the US National Library of Medicine Medical Subject Headings (MeSH) ...
B cells can present antigens to a specialized group of helper T cells called TFH cells. If an activated TFH cell recognizes the ... Roles of T cell-B-cell-activating molecule (5c8 antigen) and CD40 in contact-dependent help". Journal of Immunology. 149 (12): ... It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In ... Grewal, IS; Xu, J; Flavell, RA (7 December 1995). "Impairment of antigen-specific T-cell priming in mice lacking CD40 ligand". ...
antigen processing and presentation of peptide antigen via MHC class I. • antigen processing and presentation of exogenous ... antigen processing and presentation of exogenous peptide antigen via MHC class I. • lipoprotein transport. • negative ... peptide antigen via MHC class I, TAP-dependent. • platelet degranulation. • MyD88-dependent toll-like receptor signaling ...
Primarily, the VCAM-1 protein is an endothelial ligand for VLA-4 (Very Late Antigen-4 or integrin α4β1) of the β1 subfamily of ...
antigen binding. • virus receptor activity. • protein binding. • transmembrane signaling receptor activity. • identical protein ...
CD1 (a-c, 1A, 1D, 1E) • CD2 • CD3 (γ, δ, ε) • CD4 • CD5 • CD6 • CD7 • CD8 (a) • CD9 • CD10 • CD11 (a, b, c) • CD13 • CD14 • ... 1996). "CD88 antibodies specifically bind to C5aR on dermal CD117+ and CD14+ cells and react with a desmosomal antigen in human ...
Recently, Heneberg[17] proposed that basophils may be defined as the cellular population positive for CD13, CD44, CD54, CD63, ... pollen proteins or helminth antigens. Recent studies in mice suggest that basophils may also regulate the behavior of T cells ... When activated, some additional surface markers are known to be upregulated (CD13, CD107a, CD164), or surface-exposed (CD63, ...
CD1 (a-c, 1A, 1D, 1E) • CD2 • CD3 (γ, δ, ε) • CD4 • CD5 • CD6 • CD7 • CD8 (a) • CD9 • CD10 • CD11 (a, b, c) • CD13 • CD14 • ... 1991). „Expression of the YB5.B8 antigen (c-kit proto-oncogene product) in normal human bone marrow". Blood. 78 (1): 30-7. PMID ... 2003). „Signal transduction-associated and cell activation-linked antigens expressed in human mast cells". Int. J. Hematol. 75 ...
T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell. • T cell antigen ... CD1 (a-c, 1A, 1D, 1E) · CD2 · CD3 (γ, δ, ε) · CD4 · CD5 · CD6 · CD7 · CD8 (a) · CD9 · CD10 · CD11 (a, b, c, d) · CD13 · CD14 · ...
Eichler W, Hamann J, Aust G (Nov 1997). "Expression characteristics of the human CD97 antigen". Tissue Antigens. 50 (5): 429-38 ... Hamann J, Wishaupt JO, van Lier RA, Smeets TJ, Breedveld FC, Tak PP (Apr 1999). "Expression of the activation antigen CD97 and ... Tissue Antigens. 57 (4): 325-31. doi:10.1034/j.1399-0039.2001.057004325.x. PMID 11380941.. ... "Expression cloning and chromosomal mapping of the leukocyte activation antigen CD97, a new seven-span transmembrane molecule of ...
CD13" by people in this website by year, and whether "Antigens, CD13" was a major or minor topic of these publications. ... "Antigens, CD13" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... Below are the most recent publications written about "Antigens, CD13" by people in Profiles. ... Below are MeSH descriptors whose meaning is more general than "Antigens, CD13". ...
CD13 is involved in diverse functions, including degradation of peptide mediators, cellular adhesion, migration, viral ... CD13 is a membrane-bound ectopeptidase, highly expressed on monocytes, macrophages, and dendritic cells. ... CD13 is a membrane-bound ectopeptidase, highly expressed on monocytes, macrophages, and dendritic cells. CD13 is involved in ... CD13 mediates phagocytosis in human monocytic cells J Leukoc Biol. 2015 Jul;98(1):85-98. doi: 10.1189/jlb.2A0914-458R. Epub ...
CD13 is a 150-170 kD type II transmembrane glycoprotein also known as aminopeptidase N, APN, and gp150. ... CD13 is thought to be involved in the metabolism of many regulatory peptides and functions in antigen processing and the ... Antigen References 1. Shipp M, et al. 1993. Blood 82:1052.. 2. Larsen S, et al. 1996. J. Exp. Med. 184:183. ... Antigen Details Structure Zinc metallopeptidase, type II integral membrane glycoprotein, 150-170 kD Distribution Granulocytes, ...
The incubation of living fibroblasts with an anti-CD13 antibody on ice gave punctate labeling that was evenly distributed on ... CD13, a receptor for human coronavirus 229E (HCoV-229E), was identified as a major component of the Triton X-100-resistant ... The results indicate that HCoV-229E first binds to CD13 in the Triton X-100-resistant microdomain, then clusters CD13 by cross- ... Human coronavirus 229E binds to CD13 in rafts and enters the cell through caveolae J Virol. 2004 Aug;78(16):8701-8. doi: ...
Therefore, in Yogyakarta, Indonesia, we analyzed 239 ALL patients who were immunophenotyped including myeloid markers (CD13, ... CD33, CD117, and/or cMPO). Myeloid antigen expression was found in 25% of patients. Expression of myeloid antigen in B-lineage ... In the whole cohort of patients we did not find a significant association between myeloid antigen expression and survival, ... In conclusion, in the Indonesian ALL population, in particular, myeloid antigen-expressing T-ALL patients had a higher chance ...
We then used next-generation flow to determine antigen stability throughout the course of the disease, and found that the ... We investigated the prognostic value of those antigens required to monitor MRD in 1265 newly diagnosed patients enrolled in the ... Among different MRD techniques, flow cytometry provides additional information about antigen expression on tumor cells, which ... expression of antigens required to monitor MRD is mostly stable from diagnosis to MRD stages, except for CD81 whose expression ...
Mouse anti-Aminopeptidase N/CD13, Clone: APN/514, Novus Biologicals 0.1mg; Unlabeled Life Sciences:Antibodies:Primary ... This MAb recognizes an extracellular epitope of an integral membrane glycoprotein of 150kDa, identified as CD13. This antigen ... alanyl (membrane) aminopeptidase, Alanyl aminopeptidase, Aminopeptidase M, aminopeptidase N, AP-M, AP-N, CD13 antigen, CD13APN ... Aminopeptidase N/CD13 Monoclonal antibody specifically detects Aminopeptidase N/CD13 in Human samples. It is validated for Flow ...
Mouse monoclonal CD13 antibody [WM15] conjugated to APC. Validated in Flow Cyt and tested in Human. Immunogen corresponding to ... Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to ... Primary - Mouse Anti-CD13 antibody [WM15] (Allophycocyanin) (ab239295) Flow Cyt Protein - Recombinant human CD13 protein ( ... Flow cytometric analysis of human peripheral blood leukocytes labeling CD13 with ab239295. Surface staining. ...
Mouse monoclonal CD13 antibody [22A5]. Validated in IP, IHC, Flow Cyt and tested in Human. Cited in 1 publication(s). Immunogen ... Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to ... Protein - Recombinant human CD13 protein (ab134454) Functional Studies, SDS-PAGE Secondary - Goat Anti-Mouse IgG H&L (HRP) ( ... Primary - Mouse Anti-CD13 antibody [22A5] (ab20136) Flow Cyt, IP, IHC-Fr, IHC-P ...
Antigens, CD13 / blood*. Case-Control Studies. Catecholamines / urine. Down-Regulation. Enkephalin, Methionine / blood. ... EC 3.4.11.2/Antigens, CD13; EC 3.4.24.11/Neprilysin ...
Antigen Expression CD1a -; CD2 -; CD3 -; CD4 -; CD5 -; CD8 -; CD10 +; CD13 +; CD38 +; CD71 +; HLA DR + ... The cells are positive for the beta-2-microglobulin, Leu12, My7 (CD13), OKT9 (CD71), OKT10 (CD38) and CALLA (CD10) antigens. ...
... altered expression of CD13, CD33, CD34, CD38, CD117, and CD123; aberrant expression of lymphoid or mature myelomonocytic ... antigens on CD34(+) myeloblasts; and several marked alterations in maturing myelomonocytic cells. The data were translated into ...
Mouse Monoclonal Anti-Aminopeptidase N/CD13 Antibody (CC81) [DyLight 488]. Validated: Flow. Tested Reactivity: Bovine. 100% ... In cattle the antigen recognised by clone CC81 is primarily expressed on enterocytes and cells with a dendritic morphology in ... Additional Aminopeptidase N/CD13 Products. Aminopeptidase N/CD13 NB100-63392G * Aminopeptidase N/CD13 Antibodies ... Home » Aminopeptidase N/CD13 » Aminopeptidase N/CD13 Antibodies » Aminopeptidase N/CD13 Antibody (CC81) [DyLight 488] ...
... co-expression of myeloid antigens (CD13 and CD33) will not exclude patients; if possible, the lineage specific markers ...
... for the CD33 and CD13 antigen, respectively). Overall, the incidence of CD34+CD33+CD13+ ALLs was 10 (7%) of 150 informative ... CD13, CD65, HLA-DR antigens, and surface immunoglobulins. In addition, anti-CD20, anti-CD22, anti-CD3, anti-CD1a, anti-CD4, ... Because an increased incidence of myeloid antigen expression has been observed in Ph+ and/or BCR-ABL+ ALL,1 6 10 it has been ... A matched unrelated donor was defined as a donor-recipient pair matched for 9 or 10 of 10 HLA antigens using high-resolution ...
Myeloid plasma membrane glycoprotein CD13; p41 protein; T-cell antigen CD7; T-cell leukemia antigen; T-cell surface antigen Leu ... aminopeptidase N/CD13; ANPEP; AP-M; AP-N; Apn; CD13; CD7; CD7 antigen (p41); gp150; GP40; hAPN; LEU-9; membrane alanyl ... This antibody is a monoclonal mixture of FITC-conjugated CD7 (clone MG34, isotype IgG2a) and PE-conjugated CD13 (clone E735, ... This antibody recognizes the human CD13 cell surface glycoprotein, a 150kD molecule expressed by granulocytes and monocytes, ...
Sakai K., Hattori T., Sagawa K., Yokoyama M., Takatsuki K. Biochemical and functional characterization of MCS-2 antigen (CD13) ... 2)⇓ . The APN/CD13 gene expression ratio ranged from 0 to 2.53, with a median value of 0.50. APN/CD13 gene expression evaluated ... Correlation between APN/CD13 Expression and the IMD.. There was a significant correlation between APN/CD13 expression and the ... APN/CD13 protein was positive by immunohistochemistry in 48.0% (24 of 50) of the tumors. APN/CD13 protein was mainly found in ...
CD13 is an enzyme that is used as a biomarker to detect damage to the kidneys, and that may be used to help diagnose certain ... Defects in CD13 appear to be a cause of various types of leukemia or lymphoma. Additional information: Clone REA263 displays ... CD13 is expressed on granulocytes, myeloid progenitors, endothelial cells, epithelial cells, and subset of granular lymphoid ... REA263 recognizes the CD13 antigen, a 150-170 kDa type II transmembrane glycoprotein, which is also known as aminopeptidase N ...
... are multimerized to form the REAlease Complex to bind markers with high avidity.Clone REAL312 recognizes the CD13 antigen, a ... CD13 is an enzyme that is used as a biomarker to detect damage to the kidneys. It also serves as a receptor for one strain of ... Defects in CD13 appear to be a cause of various types of leukemia or lymphoma.The REAlease Kits consist of the respective ... CD13 is expressed on granulocytes, myeloid progenitors, endothelial cells, epithelial cells, and subset of granular lymphoid ...
CD13 Antigens (Alanine Aminopeptidase) 6. Enzymes 7. DNA (Deoxyribonucleic Acid) 8. antiarrhythmic peptide (AAP) ...
Compare and order CD13 ELISA Kits. View citations, images, detection ranges, sensitivity, prices and more. Recommended products ... CD13 Antigen Profile Antigen Summary Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and ... CD13 in Peptide Hormone Metabolism * CD13 in Peptide Hormone Metabolism * CD13 in Regulation of Systemic Arterial Blood ... aminopeptidase N/CD13 , membrane protein p161 , bAPN , cluster of differentiation antigen 13 (CD13) , kidney Zn peptidase , ...
CD13/Aminopeptidase N Is a Potential Therapeutic Target for Inflammatory Disorders Chenyang Lu, Mohammad A. Amin and David A. ... ANTIGEN RECOGNITION AND RESPONSES. *. You have accessRestricted access. AID Phosphorylation Regulates Mismatch Repair-Dependent ... Immunodominant AH1 Antigen-Deficient Necroptotic, but Not Apoptotic, Murine Cancer Cells Induce Antitumor Protection ... Capsules Equip the Human Symbiont Bacteroides thetaiotaomicron to Modulate Immune Responses to a Dominant Antigen in the ...
Anti‐CD13‐PE‐Cy5 (e.g., Beckman Coulter). * Anti‐CD19‐PE‐Cy5 (e.g., Beckman Coulter) ... The protocols can be used for detecting and enumerating human antigen‐specific T cells. Both CD8+ and CD4+ antigen‐specific T ... Phenotypic analysis of antigen‐specific T cells. Science 274:94‐96. Barouch, D.H. and Letvin, N.L. 2001. CD8+ cytotoxic T ... Cutting edge: Detection of antigen‐specific CD4+ T cells by HLA‐DR1 oligomers is dependent on the T cell activation state. J. ...
The expression of myeloid antigens CD13 and/or CD33 is a marker of ALK+ anaplastic large cell lymphomas. Am J Clin Pathol. 2008 ...
Co-expression of myeloid antigens (CD13 and CD33) allowed. - Patients with Philadelphia chromosome-positive (Ph+) ALL or bcr/ ...
Aminopeptidase N is identical to the myeloid differentiation antigen cluster of differentiation 13 (CD13). It is a zinc- ... The origin of circulating aminopeptidase N/CD13 is unclear at present. In our series, serum aminopeptidase N/CD13 levels were ... High levels of circulating aminopeptidase N/CD13 may, at least in part, reflect high levels of aminopeptidase N/CD13 expression ... Association of serum aminopeptidase N/CD13 level with clinicopathologic variables. A high serum aminopeptidase N/CD13 level was ...
One control sample showed a heterogeneous CD13 expression on monocytes; in this sample, CD13 was lower on granulocytes as well ... Both antigens are known to be phosphatidyl inositol-linked proteins that are deficient in paroxysmal nocturnal hemoglobinuria. ... The monocytic subpopulation in the MDS samples either lack expression of HLA-DR or show overexpression of this antigen or ... Aberrant expression of certain antigens was defined as more than 0.5 log different from normal expression of that specific ...
CD13 antibody LS-C759281 is an FITC-conjugated mouse monoclonal antibody to CD13 (ANPEP) from human. It is reactive with human ... ANPEP , Alanyl aminopeptidase , Aminopeptidase M , APN , Aminopeptidase N , AP-N , AP-M , CD13 , CD13 antigen , HAPN , gp150 , ... CD13 antibody LS-C759281 is an FITC-conjugated mouse monoclonal antibody to CD13 (ANPEP) from human. It is reactive with human ... Monoclonal Mouse anti‑Human ANPEP / CD13 Antibody (clone WM15, FITC) LS‑C759281 ...
Have 1+ myeloid-associated antigens (CD13, CD33, CD117). ● CD34, CD99 and HLA-DR (70%, Mod Pathol 2000;13:452). ● CD7 (30%); ...
The blasts react with antibodies to myeloperoxidase and antibodies to CD13, CD33, and CD34. Human leukocyte antigen (HLA)-DR is ... but immunophenotyping demonstrates myeloid antigens. In acute myeloblastic leukemia (M0), the blasts are agranular and ...
  • The blasts react with antibodies to myeloperoxidase and antibodies to CD13, CD33, and CD34. (wikipedia.org)
  • Unlike myeloid dendritic cells, myeloid antigens like CD11b, CD11c, CD13, CD14 and CD33 are not present on pDC surfaces. (wikipedia.org)
  • mDCs share markers in common with monocytes and granulocytes including CD13, CD33, and CD11b and perform the classical functions of DCs in taking up and presenting antigen on HLA class II molecules. (frontiersin.org)
  • In a cohort of 365 consecutively diagnosed Czech B-precursor ALL patients, we analyze distribution of MyAg+ cases and mutual relationship among CD13, CD15, CD33, CD65 and CD66c. (biomedcentral.com)
  • Erythroid precursors are classically negative for myeloid associated antigens such as CD13, CD33, and MPO. (omicsonline.org)
  • Absence of prognostic impact of CD13 and/or CD33 antigen expression in adult acute lymphoblastic leukemia. (haematologica.org)
  • RESULTS: Immunophenotyping disclosed positivity for CD33 or CD13 and for the CD34 stem cell antigen in all cases tested. (haematologica.org)
  • If the only T cell markers present are CD4 and CD7, the leukemia cells must also lack the myeloid markers CD33 and/or CD13. (mdanderson.org)
  • We here present a rare case of MPAL with t(9;22)(q34;q11.2);BCR-ABL1 and unusual B/T immunophenotype along with aberrant expression of CD13, CD33, and CD56. (springer.com)
  • IPT profile rendered a diagnosis of MPAL B/T lineage with aberrant expression of CD13, CD33, and CD56. (springer.com)
  • Flow cytometry on the CSF confirmed the presence of a myeloblast population with 14% blasts with expression of CD13, CD33, CD34, CD45 and Human Leukocyte Antigen - DR isotype (HLADR). (bmj.com)
  • Moreover, the cross-linking of CD13 with antibodies rapidly induced pSyk in human macrophages. (nih.gov)
  • Bradstock KF, Favaloro EJ, Kabral A, Kerr A, Hughes WG, Berndt MC, Musgrove E: Human myeloid differentiation antigens identified by monoclonal antibodies: expression on leukemic cells. (exbio.cz)
  • There are up to five subpopulations of CD13 molecules possessing different levels of glycosylation, which may explain the different binding patterns of various CD13 antibodies. (beckman.com)
  • Importantly, the inhibition of CD13/APN′s activity with antagonists or monoclonal antibodies inhibits tumor growth in xenograft-bearing animals, demonstrating its utility as a therapeutic angiogenic target ( 61 ). (asm.org)
  • The parameters included the ability to: form colony-forming unit fibroblasts (CFU-F), differentiate along the adipogenic and osteogenic lineages, express cell surface antigens defining the MSCs population, support normal haematopoiesis and suppress in vitro lymphocyte proliferation induced by either anti-CD3∊ plus anti-CD28 monoclonal antibodies or the mixed lymphocyte reaction. (bmj.com)
  • Our Aminopeptidase N/CD13 Antibodies can be used in a variety of model species: Bovine, Human, Mouse, Primate. (novusbio.com)
  • Choose from our Aminopeptidase N/CD13 polyclonal antibodies and browse our Aminopeptidase N/CD13 monoclonal antibody catalog. (novusbio.com)
  • A modification using precooled absolute methanol has been shown to give better results for certain cell cycle antigens such as Ki-67 and PCNA when using FITC-conjugated antibodies. (thermofisher.com)
  • The incubation of living fibroblasts with an anti-CD13 antibody on ice gave punctate labeling that was evenly distributed on the cell surface, but raising the temperature to 37 degrees C before fixation caused aggregation of the labeling. (nih.gov)
  • Aminopeptidase N/CD13 Monoclonal antibody specifically detects Aminopeptidase N/CD13 in Human samples. (fishersci.com)
  • There are currently no images for Aminopeptidase N/CD13 Antibody (NB100-63392G). (novusbio.com)
  • We previously established a novel murine monoclonal antibody(MH8-11) that recognized aminopeptidase N (APN)/cluster of differentiation antigen 13 (CD13). (aacrjournals.org)
  • Clone REAL312 is an antibody fragment derived from the full CD13 antibody molecule. (miltenyibiotec.com)
  • This antibody is a monoclonal mixture of FITC-conjugated CD7 (clone MG34, isotype IgG2a) and PE-conjugated CD13 (clone E735, isotype IgG1). (thermofisher.com)
  • This antibody recognizes the human CD13 cell surface glycoprotein, a 150kD molecule expressed by granulocytes and monocytes, and by myeloid leukemia cells. (thermofisher.com)
  • We previously developed an aminopeptidase N/CD13-specific monoclonal antibody (mAb) MH8-11, which inhibits cell motility and angiogenesis in vitro . (aacrjournals.org)
  • We previously developed an aminopeptidase N/CD13-specific monoclonal antibody (mAb) MH8-11. (aacrjournals.org)
  • CD13 antibody LS-C759281 is an FITC-conjugated mouse monoclonal antibody to CD13 (ANPEP) from human. (lsbio.com)
  • The antibody WM15 recognises an extracellular epitope of human CD13 cell surface glycoprotein, a 150 kDa molecule expressed on granulocytes, endothelial cells, epithelial cells and myeloid progenitors. (exbio.cz)
  • human kidney tissue were subjected to SDS PAGE followed by western blot with 14553-1-AP (CD13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. (ptglab.com)
  • Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14553-1-AP (CD13 antibody) at dilution of 1:200 (under 10x lens. (ptglab.com)
  • Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14553-1-AP (CD13 antibody) at dilution of 1:200 (under 40x lens. (ptglab.com)
  • Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using 14553-1-AP (CD13 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). (ptglab.com)
  • 1X10^6 HEK-293 cells were stained with 0.2ug CD13 antibody (14553-1-AP, red) and control antibody (blue). (ptglab.com)
  • The WM-15 monoclonal antibody specifically reacts with human CD13, 150-170 type II transmembrane ectoenzyme also named aminopeptidase N and gp150. (peprotech.com)
  • Each Aminopeptidase N/CD13 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. (novusbio.com)
  • Second, in an effort to focus our recombinant antibody expression efforts on those B cells that displayed evidence of clonal expansion driven by antigen stimulation, we performed deep sequencing of the Ig genes of B cells collected from seven different tumors. (cancerindex.org)
  • Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L. Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism. (umassmed.edu)
  • CD13 is a 150-170 kD type II transmembrane glycoprotein also known as aminopeptidase N, APN, and gp150. (biolegend.com)
  • CD13 is identical to aminopeptidase N (APN), a prominent membrane-bound metalloprotease present on the surface of intestinal brush border and renal tubules. (fishersci.com)
  • REA263 recognizes the CD13 antigen, a 150-170 kDa type II transmembrane glycoprotein, which is also known as aminopeptidase N or gp150. (miltenyibiotec.com)
  • 1989) Human myeloid plasma membrane glycoprotein CD13 (gp150) is identical to aminopeptidase N. J. Clin. (miltenyibiotec.com)
  • Clone REAL312 recognizes the CD13 antigen, a type II transmembrane glycoprotein, which is also known as aminopeptidase N or gp150. (miltenyibiotec.com)
  • Inhibits infection of cells by human coronavirus, inhibits aminopeptidase N activity of the CD13 molecule immunoprecipitates. (thermofisher.com)
  • Aminopeptidase N, also known as CD13, has important roles in tumor metastasis and angiogenesis. (aacrjournals.org)
  • However, the clinical significance of circulating aminopeptidase N/CD13 in patients with solid tumors is unknown. (aacrjournals.org)
  • The aim of this study was to evaluate the clinical significance of circulating aminopeptidase N/CD13 protein detected by mAb MH8-11 in patients with non-small cell lung cancer (NSCLC). (aacrjournals.org)
  • We used electrochemiluminescence immunoassay with mAb MH8-11 to determine circulating aminopeptidase N/CD13 levels in 90 healthy volunteers and 90 patients with NSCLC. (aacrjournals.org)
  • Circulating aminopeptidase N/CD13 levels were measured in sera taken before treatment and evaluated for a relationship with clinical outcomes. (aacrjournals.org)
  • A significant correlation was found between tumor progression and serum aminopeptidase N/CD13 concentrations ( r = 0.23, P = 0.029). (aacrjournals.org)
  • High serum aminopeptidase N/CD13 levels ( n = 17) were associated with advanced stage ( P = 0.004) or poor performance status ( P = 0.001). (aacrjournals.org)
  • Our data suggest that a high level of circulating aminopeptidase N/CD13 at diagnosis is an independent prognostic factor in patients with NSCLC. (aacrjournals.org)
  • Aminopeptidase N is identical to the myeloid differentiation antigen cluster of differentiation 13 (CD13). (aacrjournals.org)
  • Aminopeptidase N/CD13 is expressed in various cells outside the hematopoietic system, including epithelial cells of the intestine and kidney, synaptic membranes of the central nervous system, fibroblasts, endothelial cells, and tumor cells ( 4 , 5 ). (aacrjournals.org)
  • Aminopeptidase N/CD13 has been reported to play important roles in tumor cell invasion ( 6 , 7 ) and tumor angiogenesis ( 8 , 9 ). (aacrjournals.org)
  • In patients with resected colon or pancreatic cancer, aminopeptidase N/CD13 expression determined by immunohistochemical assay with mAb MH8-11 has been reported as an independent prognostic factor ( 10 , 11 ). (aacrjournals.org)
  • Aminopeptidase N/CD13 activity has been found to be elevated in the plasma of cancer patients and was associated with tumor load ( 12 - 14 ). (aacrjournals.org)
  • The tumor environment may be an important source of circulating aminopeptidase N/CD13. (aacrjournals.org)
  • In this report, we investigated circulating aminopeptidase N/CD13 protein concentrations detected by functional mAb MH8-11 and evaluated its clinical significance in patients with NSCLC. (aacrjournals.org)
  • We have extensively characterized the type II transmembrane metalloprotease, CD13/aminopeptidase N (APN), in angiogenic endothelial cells as one of several peptidases that play a role in angiogenesis (for a review, see reference 5 ). (asm.org)
  • Membrane-bound members of the family, with the bulk of the protein outside the cell, are also known as surface antigens, e.g. aminopeptidase N as the myeloid leukaemia marker CD13. (ebi.ac.uk)
  • The host receptor for group I CoVs, such as HCoV 229E and TGEV, is aminopeptidase N (also known as CD13) ( 4 , 21 ). (asm.org)
  • CD13, also known as aminopeptidase N, APN and gp150, is a 150-170 kDa transmembrane ectoenzyme occurring on monocytes and granulocytes but not lymphocytes in blood. (fluidigm.com)
  • Wulfaenger J, Niedling S, Riemann D, Seliger B . Aminopeptidase N (APN)/CD13-dependent CXCR4 downregulation is associated with diminished cell migration, proliferation and invasion. (uni-halle.de)
  • CD13/Aminopeptidase N (APN) is a zinc-binding, type 2 transmembrane ectopeptidase (150 kDa), which is expressed on various cell types, such as kidney, intestinal epithelium, liver, placenta and lung cells ( 14 , 15 ). (spandidos-publications.com)
  • Patients with leukemias that express the progenitor cell antigen CD34 and/or the P-glycoprotein (MDR1 gene product) have an inferior outcome. (rexhealth.com)
  • 3 These cells are identified in vitro by their ability to form colony-forming unit fibroblasts (CFU-F) and, phenotypically, by their expression of surface markers, such as CD105, CD73, CD90, integrins and adhesion molecules in addition to the absence of CD14, CD34 and CD45 haematopoietic-related antigen. (bmj.com)
  • Flow cytometric analysis revealed approximately 82% of cells in blast region expressing dim CD13, heterogeneous CD117 , moderate CD38, dim CD34, MPO positive, dim CD19, and negative for human leukocyte antigen - antigen D-related. (thefreedictionary.com)
  • This MAb recognizes an extracellular epitope of an integral membrane glycoprotein of 150kDa, identified as CD13. (fishersci.com)
  • APN/CD13 is a surface glycoprotein encoded by a gene located on the long arm of chromosome 15 at bands q25-26. (aacrjournals.org)
  • The CD13 antigen is a transmembrane glycoprotein with a large extracellular region and a small intracellular NH2-terminal tail. (beckman.com)
  • Flow cytometry analysis (surface staining) of human peripheral blood leukocytes with anti-CD13 (WM15) biotin, streptavidin-APC. (exbio.cz)
  • In cattle the antigen recognised by clone CC81 is primarily expressed on enterocytes and cells with a dendritic morphology in the small intestine. (novusbio.com)
  • Among different MRD techniques, flow cytometry provides additional information about antigen expression on tumor cells, which could potentially contribute to stratify MRD-positive patients. (nature.com)
  • The cells are positive for the beta-2-microglobulin, Leu12, My7 (CD13), OKT9 (CD71), OKT10 (CD38) and CALLA (CD10) antigens. (atcc.org)
  • in this patient, the only positive antigens identified by immunophenotyping were CD13, NG2 HLA-DR, and CD38. (sigmaaldrich.com)
  • MVs from patients specifically expressed tumor-related antigens, such as CD19 in B cell neoplasms, CD38 in MM, CD13 in myeloid tumors, and CD30 in HL. (springer.com)
  • In the quantitative comparison of myeloid antigen, the expression of CD13 and CD38 were relatively increasedin the rote non-treated cells. (nii.ac.jp)
  • CD13/APN regulates endothelial invasion and filopodia formation. (exbio.cz)
  • 2005 ). Infection by using both temperature-sensitive mutant U19tsA58 of SV40 large T antigen (SV40T) and the essential catalytic subunit of human telomerase (hTERT) has recently proven to be effective for the development of conditionally immortalized podocytes and human glomerular endothelial cells and for the immortalization of human fibroblasts (O'Hare et al. (springer.com)
  • We have shown that endothelial CD13/APN expression is induced in response to environmental angiogenic signals and that it plays an important functional role in endothelial cell invasion and morphogenesis ( 7 , 8 , 63 ). (asm.org)
  • Results: APN/CD13 gene expression detected by reverse transcriptase-PCR was positive in 50.0% (25 of 50) of the tumors, and APN/CD13 protein positive was detected by immunohistochemistry in 48.0% (24 of 50). (aacrjournals.org)
  • Efficient vaccination against infectious agents and tumors depends on specific antigen targeting to dendritic cells (DCs). (asm.org)
  • The transmembrane peptidase prostate-specific membrane antigen (PSMA) is universally upregulated in the vasculature of solid tumors, but its functional role in tumor angiogenesis has not been investigated. (asm.org)
  • Seliger B , Ruiz-Cabello F, Garrido F. IFN inducibility of major histocompatibility antigens in tumors. (uni-halle.de)
  • Seliger B . Different regulation of MHC class I antigen processing components in human tumors. (uni-halle.de)
  • Moreover, highly efficient antigen delivery to human DCs with recombinant human coronavirus 229E and specific stimulation of human CD8 + T cells revealed that this approach is exceptionally well suited for translation into human vaccine studies. (asm.org)
  • First, we cloned VH and VL genes from single intratumoral B lymphocytes isolated from one lung tumor, expressed the genes as recombinant mAbs, and used the mAbs to identify the cognate tumor antigens. (cancerindex.org)
  • This antigen is present on most cells of myeloid origin including granulocytes, monocytes, mast cells, and GM-progenitor cells. (fishersci.com)
  • CD13 is thought to be involved in the metabolism of many regulatory peptides and functions in antigen processing and the cleavage of chemokines such as MIP-1. (biolegend.com)
  • CD13 plays a role in metabolism of biologically active peptides, in phagocytosis, and in bactericidal/tumoricidal activities. (fishersci.com)
  • Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. (abcam.com)
  • CD13 plays a role in the terminal degradation of peptides, antigen processing, and chemokine cleavage, and cellular adhesion. (peprotech.com)
  • CD13 is reported to have many different functions including degradation of enkephalins and endorphins, terminal degradation of peptides, amino acid scavenging, antigen processing, and adhesion and migration of cells. (fluidigm.com)
  • NB100-63392 recognizes bovine CD13, a 150 kD type II membrane protein shown to be a metallopeptidase in humans. (novusbio.com)
  • We also used immunohistochemistry with MH8-11 to investigate APN/CD13 protein expression. (aacrjournals.org)
  • This epitope was a M r 165,000 protein, and the sequencing analysis revealed that it was almost identical to APN/CD13. (aacrjournals.org)
  • The frequency of acute lymphoblastic leukemia (ALL) patients expressing myeloid antigens on their ALL cells varies between 5 and 36% in several different studies. (hindawi.com)
  • Aberrant expression of myeloid antigens (MyAgs) on acute lymphoblastic leukemia (ALL) cells is a well-documented phenomenon, although its regulating mechanisms are unclear. (biomedcentral.com)
  • 1992). "c-kit gene expression in CD7-positive acute lymphoblastic leukemia: close correlation with expression of myeloid-associated antigen CD13. (thefullwiki.org)
  • MHV, a group II CoV ( 5 , 6 ), recognizes carcinoembryonic antigen-related cell adhesion molecules as another group of CoV receptors. (asm.org)
  • Moreover, the clinical relevance of myeloid antigen expression in childhood ALL as a prognostic factor remains controversial [ 5 , 8 - 12 ]. (hindawi.com)
  • We investigated the prognostic value of those antigens required to monitor MRD in 1265 newly diagnosed patients enrolled in the GEM2000, GEM2005MENOS65, GEM2005MAS65 and GEM2010MAS65 protocols. (nature.com)
  • However, APN/CD13 expression was not associated with various prognostic factors. (aacrjournals.org)
  • Our data suggest that APN/CD13 may be a new prognostic marker for patients with pancreatic carcinoma and may have a relationship with the angiogenesis for this cancer. (aacrjournals.org)
  • Both total and antigen-specific count of MVs significantly correlated with different HM clinical features such as Rai stage in CLL, International Prognostic Scoring System in WM, International Staging System in MM, and clinical stage in HL. (springer.com)
  • We found that hCD13 mediates efficient phagocytosis of large particles (erythrocytes) modified so as to interact with the cell only through CD13 in human macrophages and THP-1 monocytic cells. (nih.gov)
  • CD13, a receptor for human coronavirus 229E (HCoV-229E), was identified as a major component of the Triton X-100-resistant membrane microdomain in human fibroblasts. (nih.gov)
  • Tissue, cells or virus corresponding to Human CD13. (abcam.com)
  • MA1-22542 detects CD13 in Human samples. (thermofisher.com)
  • Human leukocyte antigen (HLA)-DR is positive in most patients. (wikipedia.org)
  • The protocols can be used for detecting and enumerating human antigen‐specific T cells. (currentprotocols.com)
  • Epidemiologic data suggest that certain human leukocyte antigen (HLA) types, and even certain blood types, may increase or decrease the likelihood of developing non-Hodgkin lymphoma. (medscape.com)
  • 6. The immunoglobulin of claim 4 which is immunospecific for a human antigen. (freepatentsonline.com)
  • A 32-year-old Chinese woman diagnosed with ATLL with myeloid antigen expression received HSCT from her human leukocyte antigen (HLA)-matched sister and presented with two lesions in her right breast 6 months later. (biomedcentral.com)
  • In May 2014, the patient received allogeneic HSCT from her human leukocyte antigen (HLA)-matched sister. (biomedcentral.com)
  • Here we demonstrate that CD4 is a reliable lineage marker for the human peripheral blood antigen-presenting cell compartment that can be used to identify DCs and monocytes in parallel with lymphocytes. (frontiersin.org)
  • Diminished monocyte Human Leukocyte Antigen DR (mHLA-DR) is a reliable marker of monocyte dysfunction and immunosuppression. (biomedcentral.com)
  • We evaluated changes in the expression of CD13 before and after treatment with anticancer drugs and with or without ubenimex in the human HCC cell lines HuH7 and PLC/PRF/5. (spandidos-publications.com)
  • CD13 is a membrane-bound ectopeptidase, highly expressed on monocytes, macrophages, and dendritic cells. (nih.gov)
  • This study describes a novel vaccine approach that facilitates delivery of viral or tumor antigens to dendritic cells in vivo . (asm.org)
  • Dendritic cells are highly adapted to their role of presenting antigen and directing immune responses. (biomedsearch.com)
  • Moreover, we investigated the relationship between APN/CD13 expression and tumor angiogenesis by measuring the IMD. (aacrjournals.org)
  • Recent studies have indicated that CD13 has various functions, including roles in inflammatory and immunological responses, signal transduction, antigen processing, neuropeptide and cytokine degradation, angiogenesis and extracellular matrix degradation ( 16 , 17 ). (spandidos-publications.com)
  • As mentioned earlier, maturation also induces the expression of both MHC Class I and Class II molecules in pDCs as well, which allows the cell to optimize its antigen-presenting abilities. (wikipedia.org)
  • In addition, changes in the expression of basophil surface's molecules are observed: some antigens appear de novo (CD63, CD107a), and other increase their expression (CD13, CD203c). (termedia.pl)
  • CD13 is involved in diverse functions, including degradation of peptide mediators, cellular adhesion, migration, viral endocytosis, signaling, and positive modulation of phagocytosis mediated by FcγRs and other phagocytic receptors. (nih.gov)
  • CD13-mediated phagocytosis is independent of other phagocytic receptors, as it occurs in the absence of FcγRs, CR3, and most phagocytic receptors. (nih.gov)
  • T-ALL/LBL disease is defined as: Leukemia or lymphoma cells must express at least 2 of the following cell surface antigens: CD1a, CD2, CD3 (surface or cytoplasmic), CD4, CD5, CD7, and CD8. (mdanderson.org)
  • The FIX & PERM Cell Permeabilization Kit consists of matched Fixation Reagent (Medium A) and Permeabilization Reagent (Medium B) for simultaneous analysis of intracellular and cell-surface antigens in the same cell population (Figure 1) . (thermofisher.com)
  • Leukemic presentation is very rare, and in particular, the null phenotype ALCL without typical anaplastic morphology together with aberrant expression of CD13 and/or CD11b represents a diagnostic challenge. (sigmaaldrich.com)
  • Meissner M, Whiteside TL, van Kuik-Romein P, Valesky EM, van den Elsen PJ, Kaufmann R, Seliger B . Loss of interferon-gamma inducibility of the MHC class II antigen processing pathway in head and neck cancer: evidence for post-transcriptional as well as epigenetic regulation. (uni-halle.de)
  • Recently, a second cell surface exopeptidase, prostate-specific membrane antigen (PSMA) or glutamate carboxypeptidase II, has been shown to display an expression pattern analogous to that of CD13/APN, where it is found in angiogenic but not normal tumor vasculature ( 17 , 18 , 49 , 70 ). (asm.org)
  • We report here that biosafe coronavirus-based vaccine vectors facilitate delivery of multiple antigens and immunostimulatory cytokines to professional antigen-presenting cells in vitro and in vivo . (asm.org)
  • Mixed phenotypic acute leukemia (MPAL) is an uncommon hematological neoplasm which can either contain distinct blast populations, each of a different lineage (bilineal) or one population with multiple antigens of different lineage on the same cells (biphenotypic). (springer.com)
  • Our analysis of bone marrow samples from 50 patients with MDS showed aberrant expression of differentiation antigens in the myelomonocytic lineage. (bloodjournal.org)
  • The lineage-restricted pattern of expression of CD13 within the hemopoietic compartment suggests that it may be important in myeloid cell differentiation. (fishersci.com)
  • In addition, the relevance of myeloid antigen expression on both B-lineage and T-lineage ALL patients for treatment outcome was also analyzed. (hindawi.com)
  • Granulocytic marker CD66c - Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) is aberrantly expressed on ALL with strong correlation to genotype (negative in TEL/AML1 and MLL/AF4, positive in BCR/ABL and hyperdiploid cases). (biomedcentral.com)
  • The recommended ELISA Kit will likely detect the antigen in question with higher specificity in approved samples than the available alternatives. (antibodies-online.com)
  • DCs are equipped with molecular sensors and antigen-processing machinery to recognize pathogens, integrate chemical information and guide the specificity, magnitude and polarity of immune responses. (biomedsearch.com)
  • Terauchi M, Kajiyama H, Shibata K, Ino K, Nawa A, Mizutani S, Kikkawa F. Inhibition of APN/CD13 leads to suppressed progressive potential in ovarian carcinoma cells. (exbio.cz)
  • Inhibition of CD13 induced tumor cell apoptosis and resulted in tumor disruption, via blocking the ability of dormant CSCs to self-renew and re-initiate a tumor. (spandidos-publications.com)
  • Uncommon leukemic case of anaplastic large cell lymphoma diagnosed through a typical chromosomal abnormality t(2;5) with a null phenotype and aberrant expression of NG2 and CD13. (sigmaaldrich.com)
  • Cutting edge: Detection of antigen‐specific CD4+ T cells by HLA‐DR1 oligomers is dependent on the T cell activation state. (currentprotocols.com)
  • In humans, pDCs exhibit plasma cell morphology and express CD4, HLA-DR, CD123, blood-derived dendritic cell antigen-2 (BDCA-2), Toll-like receptor (TLR) 7 and TLR9 within endosomal compartments. (wikipedia.org)
  • Several recent studies prospectively isolated and evaluated putative hepatic CSCs by using surrogate characteristics such as xenograft tumor growth, expression of stem cell-associated antigens (e.g. (aacrjournals.org)
  • Antigen-presenting cell-derived exosomes induce and tumor-derived exosomes suppress an immune response ( 17 ). (aacrjournals.org)
  • Definition of cell immunophenotype in acute leukemias is commonly employed to identify malignant line, maturation stage and finally pattern of antigen expression in individual patients what permits better monitoring of treatment and residual disease. (termedia.pl)
  • additionally, CD13 was reported to be correlated with malignant behavior in colon, prostate and non-small cell lung cancers ( 18 - 20 ). (spandidos-publications.com)
  • Use of FIX & PERM Cell Permeabilization Kit for simultaneous surface antigen and intracellular antigen staining. (thermofisher.com)
  • Rotenone,a Mitochondrial NADH Dehydrogenase Inhibitor,Induces Cell Surface Expression of CD13 and Apoptosis in HL-60 Cells. (nii.ac.jp)