Substances that are recognized by the immune system and induce an immune reaction.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
Differentiation antigens found on thymocytes and on cytotoxic and suppressor T-lymphocytes. CD8 antigens are members of the immunoglobulin supergene family and are associative recognition elements in MHC (Major Histocompatibility Complex) Class I-restricted interactions.
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
Substances elaborated by bacteria that have antigenic activity.
A bifunctional enzyme that catalyzes the synthesis and HYDROLYSIS of CYCLIC ADP-RIBOSE (cADPR) from NAD+ to ADP-RIBOSE. It is a cell surface molecule which is predominantly expressed on LYMPHOID CELLS and MYELOID CELLS.
Glycoproteins found on immature hematopoietic cells and endothelial cells. They are the only molecules to date whose expression within the blood system is restricted to a small number of progenitor cells in the bone marrow.
Differentiation antigens expressed on B-lymphocytes and B-cell precursors. They are involved in regulation of B-cell proliferation.
A member of the tumor necrosis factor receptor superfamily with specificity for CD40 LIGAND. It is found on mature B-LYMPHOCYTES and some EPITHELIAL CELLS, lymphoid DENDRITIC CELLS. Evidence suggests that CD40-dependent activation of B-cells is important for generation of memory B-cells within the germinal centers. Mutations of the gene for CD40 antigen result in HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 3. Signaling of the receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
A membrane glycoprotein and differentiation antigen expressed on the surface of T-cells that binds to CD40 ANTIGENS on B-LYMPHOCYTES and induces their proliferation. Mutation of the gene for CD40 ligand is a cause of HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 1.
Unglycosylated phosphoproteins expressed only on B-cells. They are regulators of transmembrane Ca2+ conductance and thought to play a role in B-cell activation and proliferation.
Substances elaborated by viruses that have antigenic activity.
Costimulatory T-LYMPHOCYTE receptors that have specificity for CD80 ANTIGEN and CD86 ANTIGEN. Activation of this receptor results in increased T-cell proliferation, cytokine production and promotion of T-cell survival.
Acidic sulfated integral membrane glycoproteins expressed in several alternatively spliced and variable glycosylated forms on a wide variety of cell types including mature T-cells, B-cells, medullary thymocytes, granulocytes, macrophages, erythrocytes, and fibroblasts. CD44 antigens are the principle cell surface receptors for hyaluronate and this interaction mediates binding of lymphocytes to high endothelial venules. (From Abbas et al., Cellular and Molecular Immunology, 2d ed, p156)
Differentiation antigens expressed on pluripotential hematopoietic cells, most human thymocytes, and a major subset of peripheral blood T-lymphocytes. They have been implicated in integrin-mediated cellular adhesion and as signalling receptors on T-cells.
Glycolipid-anchored membrane glycoproteins expressed on cells of the myelomonocyte lineage including monocytes, macrophages, and some granulocytes. They function as receptors for the complex of lipopolysaccharide (LPS) and LPS-binding protein.
Glycoprotein members of the immunoglobulin superfamily which participate in T-cell adhesion and activation. They are expressed on most peripheral T-lymphocytes, natural killer cells, and thymocytes, and function as co-receptors or accessory molecules in the T-cell receptor complex.
Ratio of T-LYMPHOCYTES that express the CD4 ANTIGEN to those that express the CD8 ANTIGEN. This value is commonly assessed in the diagnosis and staging of diseases affecting the IMMUNE SYSTEM including HIV INFECTIONS.
Glycoproteins expressed on all mature T-cells, thymocytes, and a subset of mature B-cells. Antibodies specific for CD5 can enhance T-cell receptor-mediated T-cell activation. The B-cell-specific molecule CD72 is a natural ligand for CD5. (From Abbas et al., Cellular and Molecular Immunology, 2d ed, p156)
Antigens expressed primarily on the membranes of living cells during sequential stages of maturation and differentiation. As immunologic markers they have high organ and tissue specificity and are useful as probes in studies of normal cell development as well as neoplastic transformation.
A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.
Glycoproteins expressed on cortical thymocytes and on some dendritic cells and B-cells. Their structure is similar to that of MHC Class I and their function has been postulated as similar also. CD1 antigens are highly specific markers for human LANGERHANS CELLS.
Antibodies produced by a single clone of cells.
The 140 kDa isoform of NCAM (neural cell adhesion molecule) containing a transmembrane domain and short cytoplasmic tail. It is expressed by all lymphocytes mediating non-MHC restricted cytotoxicity and is present on some neural tissues and tumors.
Antigens expressed on the cell membrane of T-lymphocytes during differentiation, activation, and normal and neoplastic transformation. Their phenotypic characterization is important in differential diagnosis and studies of thymic ontogeny and T-cell function.
A membrane-bound or cytosolic enzyme that catalyzes the synthesis of CYCLIC ADP-RIBOSE (cADPR) from nicotinamide adenine dinucleotide (NAD). This enzyme generally catalyzes the hydrolysis of cADPR to ADP-RIBOSE, as well, and sometimes the synthesis of cyclic ADP-ribose 2' phosphate (2'-P-cADPR) from NADP.
Surface antigens expressed on myeloid cells of the granulocyte-monocyte-histiocyte series during differentiation. Analysis of their reactivity in normal and malignant myelomonocytic cells is useful in identifying and classifying human leukemias and lymphomas.
A costimulatory ligand expressed by ANTIGEN-PRESENTING CELLS that binds to CTLA-4 ANTIGEN with high specificity and to CD28 ANTIGEN with low specificity. The interaction of CD80 with CD28 ANTIGEN provides a costimulatory signal to T-LYMPHOCYTES, while its interaction with CTLA-4 ANTIGEN may play a role in inducing PERIPHERAL TOLERANCE.
Tetraspanin proteins found at high levels in cells of the lymphoid-myeloid lineage. CD53 antigens may be involved regulating the differentiation of T-LYMPHOCYTES and the activation of B-LYMPHOCYTES.
A cell adhesion protein that was originally identified as a heat stable antigen in mice. It is involved in METASTASIS and is highly expressed in many NEOPLASMS.
Zinc-binding metalloproteases that are members of the type II integral membrane metalloproteases. They are expressed by GRANULOCYTES; MONOCYTES; and their precursors as well as by various non-hematopoietic cells. They release an N-terminal amino acid from a peptide, amide or arylamide.
Any part or derivative of any protozoan that elicits immunity; malaria (Plasmodium) and trypanosome antigens are presently the most frequently encountered.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
A costimulatory ligand expressed by ANTIGEN-PRESENTING CELLS that binds to CD28 ANTIGEN with high specificity and to CTLA-4 ANTIGEN with low specificity. The interaction of CD86 with CD28 ANTIGEN provides a stimulatory signal to T-LYMPHOCYTES, while its interaction with CTLA-4 ANTIGEN may play a role in inducing PERIPHERAL TOLERANCE.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.
A tumor necrosis factor receptor subtype found in a variety of tissues and on activated LYMPHOCYTES. It has specificity for FAS LIGAND and plays a role in regulation of peripheral immune responses and APOPTOSIS. Multiple isoforms of the protein exist due to multiple ALTERNATIVE SPLICING. The activated receptor signals via a conserved death domain that associates with specific TNF RECEPTOR-ASSOCIATED FACTORS in the CYTOPLASM.
Antigens determined by leukocyte loci found on chromosome 6, the major histocompatibility loci in humans. They are polypeptides or glycoproteins found on most nucleated cells and platelets, determine tissue types for transplantation, and are associated with certain diseases.
Membrane antigens associated with maturation stages of B-lymphocytes, often expressed in tumors of B-cell origin.
High-molecular weight glycoproteins uniquely expressed on the surface of LEUKOCYTES and their hemopoietic progenitors. They contain a cytoplasmic protein tyrosine phosphatase activity which plays a role in intracellular signaling from the CELL SURFACE RECEPTORS. The CD45 antigens occur as multiple isoforms that result from alternative mRNA splicing and differential usage of three exons.
Process of classifying cells of the immune system based on structural and functional differences. The process is commonly used to analyze and sort T-lymphocytes into subsets based on CD antigens by the technique of flow cytometry.
Substances of fungal origin that have antigenic activity.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The major group of transplantation antigens in the mouse.
A 67-kDa sialic acid binding lectin that is specific for MYELOID CELLS and MONOCYTE-MACROPHAGE PRECURSOR CELLS. This protein is the smallest siglec subtype and contains a single immunoglobulin C2-set domain. It may play a role in intracellular signaling via its interaction with SHP-1 PROTEIN-TYROSINE PHOSPHATASE and SHP-2 PROTEIN-TYROSINE PHOSPHATASE.
Any part or derivative of a helminth that elicits an immune reaction. The most commonly seen helminth antigens are those of the schistosomes.
Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.
Cell-surface glycoprotein beta-chains that are non-covalently linked to specific alpha-chains of the CD11 family of leukocyte-adhesion molecules (RECEPTORS, LEUKOCYTE-ADHESION). A defect in the gene encoding CD18 causes LEUKOCYTE-ADHESION DEFICIENCY SYNDROME.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
A member of the tumor necrosis factor receptor superfamily that may play a role in the regulation of NF-KAPPA B and APOPTOSIS. They are found on activated T-LYMPHOCYTES; B-LYMPHOCYTES; NEUTROPHILS; EOSINOPHILS; MAST CELLS and NK CELLS. Overexpression of CD30 antigen in hematopoietic malignancies make the antigen clinically useful as a biological tumor marker. Signaling of the receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
Glycoproteins found on the membrane or surface of cells.
A critical subpopulation of regulatory T-lymphocytes involved in MHC Class I-restricted interactions. They include both cytotoxic T-lymphocytes (T-LYMPHOCYTES, CYTOTOXIC) and CD8+ suppressor T-lymphocytes.
Sites on an antigen that interact with specific antibodies.
A subtype of tetraspanin proteins that play a role in cell adhesion, cell motility, and tumor metastasis. CD9 antigens take part in the process of platelet activation and aggregation, the formation of paranodal junctions in neuronal tissue, and the fusion of sperm with egg.
A glycoprotein that is secreted into the luminal surface of the epithelia in the gastrointestinal tract. It is found in the feces and pancreaticobiliary secretions and is used to monitor the response to colon cancer treatment.
A subclass of HLA-D antigens that consist of alpha and beta chains. The inheritance of HLA-DR antigens differs from that of the HLA-DQ ANTIGENS and HLA-DP ANTIGENS.
A trisaccharide antigen expressed on glycolipids and many cell-surface glycoproteins. In the blood the antigen is found on the surface of NEUTROPHILS; EOSINOPHILS; and MONOCYTES. In addition, CD15 antigen is a stage-specific embryonic antigen.
Those proteins recognized by antibodies from serum of animals bearing tumors induced by viruses; these proteins are presumably coded for by the nucleic acids of the same viruses that caused the neoplastic transformation.
Established cell cultures that have the potential to propagate indefinitely.
A sialic acid-rich protein and an integral cell membrane mucin. It plays an important role in activation of T-LYMPHOCYTES.
Leukocyte differentiation antigens and major platelet membrane glycoproteins present on MONOCYTES; ENDOTHELIAL CELLS; PLATELETS; and mammary EPITHELIAL CELLS. They play major roles in CELL ADHESION; SIGNAL TRANSDUCTION; and regulation of angiogenesis. CD36 is a receptor for THROMBOSPONDINS and can act as a scavenger receptor that recognizes and transports oxidized LIPOPROTEINS and FATTY ACIDS.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A group of three different alpha chains (CD11a, CD11b, CD11c) that are associated with an invariant CD18 beta chain (ANTIGENS, CD18). The three resulting leukocyte-adhesion molecules (RECEPTORS, LEUKOCYTE ADHESION) are LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN-1; MACROPHAGE-1 ANTIGEN; and ANTIGEN, P150,95.
Large, transmembrane, non-covalently linked glycoproteins (alpha and beta). Both chains can be polymorphic although there is more structural variation in the beta chains. The class II antigens in humans are called HLA-D ANTIGENS and are coded by a gene on chromosome 6. In mice, two genes named IA and IE on chromosome 17 code for the H-2 antigens. The antigens are found on B-lymphocytes, macrophages, epidermal cells, and sperm and are thought to mediate the competence of and cellular cooperation in the immune response. The term IA antigens used to refer only to the proteins encoded by the IA genes in the mouse, but is now used as a generic term for any class II histocompatibility antigen.
A group of antigens that includes both the major and minor histocompatibility antigens. The former are genetically determined by the major histocompatibility complex. They determine tissue type for transplantation and cause allograft rejections. The latter are systems of allelic alloantigens that can cause weak transplant rejection.
Small glycoproteins found on both hematopoietic and non-hematopoietic cells. CD59 restricts the cytolytic activity of homologous complement by binding to C8 and C9 and blocking the assembly of the membrane attack complex. (From Barclay et al., The Leukocyte Antigen FactsBook, 1993, p234)
IMMUNOGLOBULINS on the surface of B-LYMPHOCYTES. Their MESSENGER RNA contains an EXON with a membrane spanning sequence, producing immunoglobulins in the form of type I transmembrane proteins as opposed to secreted immunoglobulins (ANTIBODIES) which do not contain the membrane spanning segment.
Nuclear antigen with a role in DNA synthesis, DNA repair, and cell cycle progression. PCNA is required for the coordinated synthesis of both leading and lagging strands at the replication fork during DNA replication. PCNA expression correlates with the proliferation activity of several malignant and non-malignant cell types.
Oligosaccharide antigenic determinants found principally on NK cells and T-cells. Their role in the immune response is poorly understood.
A transmembrane protein belonging to the tumor necrosis factor superfamily that specifically binds to CD27 ANTIGEN. It is found on activated T-LYMPHOCYTES; B-LYMPHOCYTES; and DENDRITIC CELLS where it plays a role in stimulating the proliferation of CD4-POSITIVE T-LYMPHOCYTES and CD8-POSITIVE T-LYMPHOCYTES.
A ubiquitously expressed complement receptor that binds COMPLEMENT C3B and COMPLEMENT C4B and serves as a cofactor for their inactivation. CD46 also interacts with a wide variety of pathogens and mediates immune response.
A class of animal lectins that bind to carbohydrate in a calcium-dependent manner. They share a common carbohydrate-binding domain that is structurally distinct from other classes of lectins.
Glycoproteins with a wide distribution on hematopoietic and non-hematopoietic cells and strongly expressed on macrophages. CD58 mediates cell adhesion by binding to CD2; (ANTIGENS, CD2); and this enhances antigen-specific T-cell activation.
55-kDa antigens found on HELPER-INDUCER T-LYMPHOCYTES and on a variety of other immune cell types. CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in MAJOR HISTOCOMPATIBILITY COMPLEX class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as INTERLEUKIN-15 receptors and bind to the HIV receptors, binding directly to the HIV ENVELOPE PROTEIN GP120.
A ubiquitously expressed membrane glycoprotein. It interacts with a variety of INTEGRINS and mediates responses to EXTRACELLULAR MATRIX PROTEINS.
A CD antigen that contains a conserved I domain which is involved in ligand binding. When combined with CD18 the two subunits form MACROPHAGE-1 ANTIGEN.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A glycoprotein that is a kallikrein-like serine proteinase and an esterase, produced by epithelial cells of both normal and malignant prostate tissue. It is an important marker for the diagnosis of prostate cancer.
An integrin alpha subunit of approximately 150-kDa molecular weight. It is expressed at high levels on monocytes and combines with CD18 ANTIGEN to form the cell surface receptor INTEGRIN ALPHAXBETA2. The subunit contains a conserved I-domain which is characteristic of several of alpha integrins.
The lipopolysaccharide-protein somatic antigens, usually from gram-negative bacteria, important in the serological classification of enteric bacilli. The O-specific chains determine the specificity of the O antigens of a given serotype. O antigens are the immunodominant part of the lipopolysaccharide molecule in the intact bacterial cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
A specific HLA-A surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-A*02 allele family.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Progenitor cells from which all blood cells derive.
The number of CD4-POSITIVE T-LYMPHOCYTES per unit volume of BLOOD. Determination requires the use of a fluorescence-activated flow cytometer.
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
Carbohydrate antigens expressed by malignant tissue. They are useful as tumor markers and are measured in the serum by means of a radioimmunoassay employing monoclonal antibodies.
GPI-linked membrane proteins broadly distributed among hematopoietic and non-hematopoietic cells. CD55 prevents the assembly of C3 CONVERTASE or accelerates the disassembly of preformed convertase, thus blocking the formation of the membrane attack complex.
Cell adhesion molecules present on virtually all monocytes, platelets, and granulocytes. CD31 is highly expressed on endothelial cells and concentrated at the junctions between them.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Membrane glycoproteins consisting of an alpha subunit and a BETA 2-MICROGLOBULIN beta subunit. In humans, highly polymorphic genes on CHROMOSOME 6 encode the alpha subunits of class I antigens and play an important role in determining the serological specificity of the surface antigen. Class I antigens are found on most nucleated cells and are generally detected by their reactivity with alloantisera. These antigens are recognized during GRAFT REJECTION and restrict cell-mediated lysis of virus-infected cells.
Tetraspanin proteins that are involved in a variety of cellular functions including BASEMENT MEMBRANE assembly, and in the formation of a molecular complexes on the surface of LYMPHOCYTES.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
A member of the tumor necrosis factor receptor superfamily that is specific for 4-1BB LIGAND. It is found in a variety of immune cell types including activated T-LYMPHOCYTES; NATURAL KILLER CELLS; and DENDRITIC CELLS. Activation of the receptor on T-LYMPHOCYTES plays a role in their expansion, production of cytokines and survival. Signaling by the activated receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Proteins prepared by recombinant DNA technology.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.
Polymorphic class I human histocompatibility (HLA) surface antigens present on almost all nucleated cells. At least 20 antigens have been identified which are encoded by the A locus of multiple alleles on chromosome 6. They serve as targets for T-cell cytolytic responses and are involved with acceptance or rejection of tissue/organ grafts.
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).
Receptors present on activated T-LYMPHOCYTES and B-LYMPHOCYTES that are specific for INTERLEUKIN-2 and play an important role in LYMPHOCYTE ACTIVATION. They are heterotrimeric proteins consisting of the INTERLEUKIN-2 RECEPTOR ALPHA SUBUNIT, the INTERLEUKIN-2 RECEPTOR BETA SUBUNIT, and the INTERLEUKIN RECEPTOR COMMON GAMMA-CHAIN.
Sets of cell surface antigens located on BLOOD CELLS. They are usually membrane GLYCOPROTEINS or GLYCOLIPIDS that are antigenically distinguished by their carbohydrate moieties.
Those hepatitis B antigens found on the surface of the Dane particle and on the 20 nm spherical and tubular particles. Several subspecificities of the surface antigen are known. These were formerly called the Australia antigen.
Ubiquitously-expressed tetraspanin proteins that are found in late ENDOSOMES and LYSOSOMES and have been implicated in intracellular transport of proteins.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Tetraspanin proteins found associated with LAMININ-binding INTEGRINS. The CD151 antigens may play a role in the regulation of CELL MOTILITY.
A component of the B-cell antigen receptor that is involved in B-cell antigen receptor heavy chain transport to the PLASMA MEMBRANE. It is expressed almost exclusively in B-LYMPHOCYTES and serves as a useful marker for B-cell NEOPLASMS.
An encapsulated lymphatic organ through which venous blood filters.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Human immune-response or Class II antigens found mainly, but not exclusively, on B-lymphocytes and produced from genes of the HLA-D locus. They are extremely polymorphic families of glycopeptides, each consisting of two chains, alpha and beta. This group of antigens includes the -DR, -DQ and -DP designations, of which HLA-DR is most studied; some of these glycoproteins are associated with certain diseases, possibly of immune etiology.
A membrane-bound tumor necrosis family member found primarily on activated T-LYMPHOCYTES that binds specifically to CD30 ANTIGEN. It may play a role in INFLAMMATION and immune regulation.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
A class of enzymes involved in the hydrolysis of the N-glycosidic bond of nitrogen-linked sugars.
A form of undifferentiated malignant LYMPHOMA usually found in central Africa, but also reported in other parts of the world. It is commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. B-cell antigens are expressed on the immature cells that make up the tumor in virtually all cases of Burkitt lymphoma. The Epstein-Barr virus (HERPESVIRUS 4, HUMAN) has been isolated from Burkitt lymphoma cases in Africa and it is implicated as the causative agent in these cases; however, most non-African cases are EBV-negative.
Molecules on the surface of B- and T-lymphocytes that recognize and combine with specific antigens.
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.
An alpha-integrin subunit found on lymphocytes, granulocytes, macrophages and monocytes. It combines with the integrin beta2 subunit (CD18 ANTIGEN) to form LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN-1.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Antigens of the virion of the HEPATITIS B VIRUS or the Dane particle, its surface (HEPATITIS B SURFACE ANTIGENS), core (HEPATITIS B CORE ANTIGENS), and other associated antigens, including the HEPATITIS B E ANTIGENS.
The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.
The processes triggered by interactions of ANTIBODIES with their ANTIGENS.
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
Mice homozygous for the mutant autosomal recessive gene "scid" which is located on the centromeric end of chromosome 16. These mice lack mature, functional lymphocytes and are thus highly susceptible to lethal opportunistic infections if not chronically treated with antibiotics. The lack of B- and T-cell immunity resembles severe combined immunodeficiency (SCID) syndrome in human infants. SCID mice are useful as animal models since they are receptive to implantation of a human immune system producing SCID-human (SCID-hu) hematochimeric mice.
Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
A heterogeneous group of immunocompetent cells that mediate the cellular immune response by processing and presenting antigens to the T-cells. Traditional antigen-presenting cells include MACROPHAGES; DENDRITIC CELLS; LANGERHANS CELLS; and B-LYMPHOCYTES. FOLLICULAR DENDRITIC CELLS are not traditional antigen-presenting cells, but because they hold antigen on their cell surface in the form of IMMUNE COMPLEXES for B-cell recognition they are considered so by some authors.
The type species of LYMPHOCRYPTOVIRUS, subfamily GAMMAHERPESVIRINAE, infecting B-cells in humans. It is thought to be the causative agent of INFECTIOUS MONONUCLEOSIS and is strongly associated with oral hairy leukoplakia (LEUKOPLAKIA, HAIRY;), BURKITT LYMPHOMA; and other malignancies.
T-cell receptors composed of CD3-associated alpha and beta polypeptide chains and expressed primarily in CD4+ or CD8+ T-cells. Unlike immunoglobulins, the alpha-beta T-cell receptors recognize antigens only when presented in association with major histocompatibility (MHC) molecules.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Class I human histocompatibility (HLA) surface antigens encoded by more than 30 detectable alleles on locus B of the HLA complex, the most polymorphic of all the HLA specificities. Several of these antigens (e.g., HLA-B27, -B7, -B8) are strongly associated with predisposition to rheumatoid and other autoimmune disorders. Like other class I HLA determinants, they are involved in the cellular immune reactivity of cytolytic T lymphocytes.
The altered state of immunologic responsiveness resulting from initial contact with antigen, which enables the individual to produce antibodies more rapidly and in greater quantity in response to secondary antigenic stimulus.
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
The phenomenon of target cell destruction by immunologically active effector cells. It may be brought about directly by sensitized T-lymphocytes or by lymphoid or myeloid "killer" cells, or it may be mediated by cytotoxic antibody, cytotoxic factor released by lymphoid cells, or complement.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
A melanosome-specific protein that plays a role in the expression, stability, trafficking, and processing of GP100 MELANOMA ANTIGEN, which is critical to the formation of Stage II MELANOSOMES. The protein is used as an antigen marker for MELANOMA cells.
A widely distributed cell surface transmembrane glycoprotein that stimulates the synthesis of MATRIX METALLOPROTEINASES. It is found at high levels on the surface of malignant NEOPLASMS and may play a role as a mediator of malignant cell behavior.
A general term for various neoplastic diseases of the lymphoid tissue.
An albumin obtained from the white of eggs. It is a member of the serpin superfamily.
Antigens associated with specific proteins of the human adult T-cell immunodeficiency virus (HIV); also called HTLV-III-associated and lymphadenopathy-associated virus (LAV) antigens.
An inhibitory T CELL receptor that is closely related to CD28 ANTIGEN. It has specificity for CD80 ANTIGEN and CD86 ANTIGEN and acts as a negative regulator of peripheral T cell function. CTLA-4 antigen is believed to play role in inducing PERIPHERAL TOLERANCE.
A promyelocytic cell line derived from a patient with ACUTE PROMYELOCYTIC LEUKEMIA. HL-60 cells lack specific markers for LYMPHOID CELLS but express surface receptors for FC FRAGMENTS and COMPLEMENT SYSTEM PROTEINS. They also exhibit phagocytic activity and responsiveness to chemotactic stimuli. (From Hay et al., American Type Culture Collection, 7th ed, pp127-8)
A widely expressed transmembrane glycoprotein that functions as a METASTASIS suppressor protein. It is underexpressed in a variety of human NEOPLASMS.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
A group of differentiation surface antigens, among the first to be discovered on thymocytes and T-lymphocytes. Originally identified in the mouse, they are also found in other species including humans, and are expressed on brain neurons and other cells.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
The specific failure of a normally responsive individual to make an immune response to a known antigen. It results from previous contact with the antigen by an immunologically immature individual (fetus or neonate) or by an adult exposed to extreme high-dose or low-dose antigen, or by exposure to radiation, antimetabolites, antilymphocytic serum, etc.
Manifestations of the immune response which are mediated by antigen-sensitized T-lymphocytes via lymphokines or direct cytotoxicity. This takes place in the absence of circulating antibody or where antibody plays a subordinate role.
A single, unpaired primary lymphoid organ situated in the MEDIASTINUM, extending superiorly into the neck to the lower edge of the THYROID GLAND and inferiorly to the fourth costal cartilage. It is necessary for normal development of immunologic function early in life. By puberty, it begins to involute and much of the tissue is replaced by fat.
Endogenous tissue constituents that have the ability to interact with AUTOANTIBODIES and cause an immune response.
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
Nuclear antigens encoded by VIRAL GENES found in HUMAN HERPESVIRUS 4. At least six nuclear antigens have been identified.
A soluble substance elaborated by antigen- or mitogen-stimulated T-LYMPHOCYTES which induces DNA synthesis in naive lymphocytes.
A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.
A cell line derived from cultured tumor cells.
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
A sex-specific cell surface antigen produced by the sex-determining gene of the Y chromosome in mammals. It causes syngeneic grafts from males to females to be rejected and interacts with somatic elements of the embryologic undifferentiated gonad to produce testicular organogenesis.
A cell adhesion molecule of the immunoglobulin superfamily that is expressed in ENDOTHELIAL CELLS and is involved in INTERCELLULAR JUNCTIONS.
Antigens stimulating the formation of, or combining with heterophile antibodies. They are cross-reacting antigens found in phylogenetically unrelated species.
CD4-positive T cells that inhibit immunopathology or autoimmune disease in vivo. They inhibit the immune response by influencing the activity of other cell types. Regulatory T-cells include naturally occurring CD4+CD25+ cells, IL-10 secreting Tr1 cells, and Th3 cells.
Antibodies obtained from a single clone of cells grown in mice or rats.
Antigenic determinants recognized and bound by the T-cell receptor. Epitopes recognized by the T-cell receptor are often located in the inner, unexposed side of the antigen, and become accessible to the T-cell receptors after proteolytic processing of the antigen.
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
A heterodimeric protein that is a cell surface antigen associated with lymphocyte activation. The initial characterization of this protein revealed one identifiable heavy chain (ANTIGENS, CD98 HEAVY CHAIN) and an indeterminate smaller light chain. It is now known that a variety of light chain subunits (ANTIGENS, CD98 LIGHT CHAINS) can dimerize with the heavy chain. Depending upon its light chain composition a diverse array of functions can be found for this protein. Functions include: type L amino acid transport, type y+L amino acid transport and regulation of cellular fusion.
The hepatitis B antigen within the core of the Dane particle, the infectious hepatitis virion.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
The complex formed by the binding of antigen and antibody molecules. The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES.
They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system.
The sum of the weight of all the atoms in a molecule.
Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.
A group of the D-related HLA antigens found to differ from the DR antigens in genetic locus and therefore inheritance. These antigens are polymorphic glycoproteins comprising alpha and beta chains and are found on lymphoid and other cells, often associated with certain diseases.
Immunoglobulins produced in response to VIRAL ANTIGENS.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
A glycolipid, cross-species antigen that induces production of antisheep hemolysin. It is present on the tissue cells of many species but absent in humans. It is found in many infectious agents.
Elements of limited time intervals, contributing to particular results or situations.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
An inhibitory B7 antigen that has specificity for the T-CELL receptor PROGRAMMED CELL DEATH 1 PROTEIN. CD274 antigen provides negative signals that control and inhibit T-cell responses and is found at higher than normal levels on tumor cells, suggesting its potential role in TUMOR IMMUNE EVASION.
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
A species of POLYOMAVIRUS originally isolated from Rhesus monkey kidney tissue. It produces malignancy in human and newborn hamster kidney cell cultures.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
Substances that augment, stimulate, activate, potentiate, or modulate the immune response at either the cellular or humoral level. The classical agents (Freund's adjuvant, BCG, Corynebacterium parvum, et al.) contain bacterial antigens. Some are endogenous (e.g., histamine, interferon, transfer factor, tuftsin, interleukin-1). Their mode of action is either non-specific, resulting in increased immune responsiveness to a wide variety of antigens, or antigen-specific, i.e., affecting a restricted type of immune response to a narrow group of antigens. The therapeutic efficacy of many biological response modifiers is related to their antigen-specific immunoadjuvanticity.
Antigens that exist in alternative (allelic) forms in a single species. When an isoantigen is encountered by species members who lack it, an immune response is induced. Typical isoantigens are the BLOOD GROUP ANTIGENS.
Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell.
A melanosome-associated protein that plays a role in the maturation of the MELANOSOME.
The genetic region which contains the loci of genes which determine the structure of the serologically defined (SD) and lymphocyte-defined (LD) TRANSPLANTATION ANTIGENS, genes which control the structure of the IMMUNE RESPONSE-ASSOCIATED ANTIGENS, HUMAN; the IMMUNE RESPONSE GENES which control the ability of an animal to respond immunologically to antigenic stimuli, and genes which determine the structure and/or level of the first four components of complement.
Bone marrow-derived lymphocytes that possess cytotoxic properties, classically directed against transformed and virus-infected cells. Unlike T CELLS; and B CELLS; NK CELLS are not antigen specific. The cytotoxicity of natural killer cells is determined by the collective signaling of an array of inhibitory and stimulatory CELL SURFACE RECEPTORS. A subset of T-LYMPHOCYTES referred to as NATURAL KILLER T CELLS shares some of the properties of this cell type.
A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
Form of passive immunization where previously sensitized immunologic agents (cells or serum) are transferred to non-immune recipients. When transfer of cells is used as a therapy for the treatment of neoplasms, it is called adoptive immunotherapy (IMMUNOTHERAPY, ADOPTIVE).

Reduced up-regulation of memory and adhesion/integrin molecules in susceptible mice and poor expression of immunity to pulmonary tuberculosis. (1/124)

Previous studies examining the expression of adhesion and integrin molecules on CD4 T lymphocytes generated in response to virulent Mycobacterium tuberculosis infection revealed that certain inbred mouse strains susceptible to breakdown of chronic disease and subsequent reactivation had poor expression of these molecules, which might underlie their inability to adequately focus into lung tissues and mediate protection. The current study examines the possibility that prior vaccination with BCG, or a prior tuberculosis infection, would overcome this deficiency. It was found, however, that this was not the case. Whereas both resistant (C57BL/6) and susceptible (DBA/2, CBA/J) strains were equally well protected in the spleen after intravenous challenge, the latter strains were poorly protected in the lungs regardless of whether the challenge was given by the intravenous or aerosol route. Again, this was associated with poor up-regulation of adhesion and integrin molecules and with histological evidence in memory immune animals of a reduced and delayed influx of T lymphocytes into the lungs.  (+info)

In vivo IL-10 production reactivates chronic pulmonary tuberculosis in C57BL/6 mice. (2/124)

The production of immunosuppressive cytokines, such as IL-10 and TGF-beta, has been documented in individuals diagnosed with active tuberculosis. In addition, IL-10 production is increased within the lungs of mice that have chronic mycobacterial infection. Therefore, we hypothesized that the down-regulatory properties of IL-10 might contribute to the reactivation of chronic Mycobacterium tuberculosis infection in mice. To determine the influence of IL-10 on the course of infection, transgenic mice producing increased amounts of IL-10 under the control of the IL-2 promotor were infected with M. tuberculosis via the respiratory route. Mice that overexpressed IL-10 showed no increase in susceptibility during the early stages of infection, but during the chronic phase of the infection showed evidence of reactivation tuberculosis with a highly significant increase in bacterial numbers within the lungs. Reactivation was associated with the formation of macrophage-dominated lesions, decreased mRNA production for TNF and IL-12p40, and a decrease in Ag-specific IFN-gamma secretion. These data support the hypothesis that IL-10 plays a pivotal role during the chronic/latent stage of pulmonary tuberculosis, with increased production playing a potentially central role in promoting reactivation tuberculosis.  (+info)

ICSBP is essential for the development of mouse type I interferon-producing cells and for the generation and activation of CD8alpha(+) dendritic cells. (3/124)

Interferon (IFN) consensus sequence-binding protein (ICSBP) is a transcription factor playing a critical role in the regulation of lineage commitment, especially in myeloid cell differentiation. In this study, we have characterized the phenotype and activation pattern of subsets of dendritic cells (DCs) in ICSBP(-/-) mice. Remarkably, the recently identified mouse IFN-producing cells (mIPCs) were absent in all lymphoid organs from ICSBP(-/-) mice, as revealed by lack of CD11c(low)B220(+)Ly6C(+)CD11b(-) cells. In parallel, CD11c(+) cells isolated from ICSBP(-/-) spleens were unable to produce type I IFNs in response to viral stimulation. ICSBP(-/-) mice also displayed a marked reduction of the DC subset expressing the CD8alpha marker (CD8alpha(+) DCs) in spleen, lymph nodes, and thymus. Moreover, ICSBP(-/-) CD8alpha(+) DCs exhibited a markedly impaired phenotype when compared with WT DCs. They expressed very low levels of costimulatory molecules (intercellular adhesion molecule [ICAM]-1, CD40, CD80, CD86) and of the T cell area-homing chemokine receptor CCR7, whereas they showed higher levels of CCR2 and CCR6, as revealed by reverse transcription PCR. In addition, these cells were unable to undergo full phenotypic activation upon in vitro culture in presence of maturation stimuli such as lipopolysaccharide or poly (I:C), which paralleled with lack of Toll-like receptor (TLR)3 mRNA expression. Finally, cytokine expression pattern was also altered in ICSBP(-/-) DCs, as they did not express interleukin (IL)-12p40 or IL-15, but they displayed detectable IL-4 mRNA levels. On the whole, these results indicate that ICSBP is a crucial factor in the regulation of two possibly linked processes: (a) the development and activity of mIPCs, whose lack in ICSBP(-/-) mice may explain their high susceptibility to virus infections; (b) the generation and activation of CD8alpha(+) DCs, whose impairment in ICSBP(-/-) mice can be responsible for the defective generation of a Th1 type of immune response.  (+info)

Roles of neutrophil beta 2 integrins in kinetics of bacteremia, extravasation, and tick acquisition of Anaplasma phagocytophila in mice. (4/124)

Tick saliva contains anti-inflammatory and immunosuppressive substances that facilitate blood feeding and enhance tick-vectored pathogen transmission, including Anaplasma phagocytophila an etiologic agent of granulocytic ehrlichiosis. As such, inflammation at a tick-feeding site is strikingly different than that typically observed at other sites of inflammation. Up-regulation of CD11b/CD18 occurs in host granulocytes following interaction or infection with A phagocytophila, and the absence of CD11b/CD18 results in early increases in bacteremia. We hypothesized that beta 2 integrin-dependent infection kinetics and leukocyte extravasation are important determinants of neutrophil trafficking to, and pathogen acquisition at, tick-feeding sites. A phagocytophila infection kinetics were evaluated in CD11a/CD18, CD11b/CD18, and CD18 knock-out mice using quantitative polymerase chain reaction (PCR) of blood, ticks, and skin biopsies in conjunction with histopathology. A marked increase in the rate of A phagocytophila infection of neutrophils and pathogen burden in blood followed tick feeding. Infection kinetics were modified by beta 2 integrin expression and systemic neutrophil counts. Significant neutrophil-pathogen trafficking was observed to both suture and tick sites. Despite the prominent role for beta 2 integrins in neutrophil arrest in flowing blood, successful pathogen acquisition by ticks occurred in the absence of beta 2 integrins. Establishment of feeding pools that rely less on leukocyte trafficking and more on small hemorrhages may explain the ready amplification of A phagocytophila DNA from ticks infested on CD11/CD18-deficient mouse strains.  (+info)

Regulation of cellular adhesion molecule expression in murine oocytes, peri-implantation and post-implantation embryos. (5/124)

Expression of the adhesion molecules, ICAM-1, VCAM-1, NCAM, CD44, CD49d (VLA-4, alpha chain), and CD11a (LFA-1, alpha chain) on mouse oocytes, and pre- and peri-implantation stage embryos was examined by quantitative indirect immunofluorescence microscopy. ICAM-1 was most strongly expressed at the oocyte stage, gradually declining almost to undetectable levels by the expanded blastocyst stage. NCAM, also expressed maximally on the oocyte, declined to undetectable levels beyond the morula stage. On the other hand, CD44 declined from highest expression at the oocyte stage to show a second maximum at the compacted 8-cell/morula. This molecule exhibited high expression around contact areas between trophectoderm and zona pellucida during blastocyst hatching. CD49d was highly expressed in the oocyte, remained significantly expressed throughout and after blastocyst hatching was expressed on the polar trophectoderm. Like CD44, CD49d declined to undetectable levels at the blastocyst outgrowth stage. Expression of both VCAM-1 and CD11a was undetectable throughout. The diametrical temporal expression pattern of ICAM-1 and NCAM compared to CD44 and CD49d suggest that dynamic changes in expression of adhesion molecules may be important for interaction of the embryo with the maternal cellular environment as well as for continuing development and survival of the early embryo.  (+info)

Ly6C induces clustering of LFA-1 (CD11a/CD18) and is involved in subtype-specific adhesion of CD8 T cells. (6/124)

Ly6C is a hemopoietic cell differentiation Ag found on a subset of CD8 T cells in the periphery. It is involved in target cell killing by CTLs, augments TCR-mediated activation of IL-2 and IFN-gamma production in CD8 T cells, and regulates CD8 T cell homing in vivo. In this study, we show that cross-linking of Ly6C causes clustering of LFA-1 (CD11a/CD18) on the surface of CD8 T cells via a mechanism dependent on reorganization of actin cytoskeleton and intracellular protease, calpain, but not the phosphatidylinositol 3-kinase pathway. In the capillary flow-adhesion assay, Ly6C cross-linking significantly augments lymphocyte adhesion to endothelium, and this is inhibited by an Ab that blocks LFA-1 function. Furthermore, upon in vitro cross-linking and during in vivo homing into lymph nodes, Ly6C is transiently lost from cell surface but becomes re-expressed on lymph node-resident CD8 T cells. The abilities of Ly6C to induce LFA-1 clustering and to be re-expressed after signaling-associated down-regulation may be important in regulating the homing of CD8 T cells into lymph nodes and in subsequent steps of CD8 T cell activation and effector function that again involve LFA-1.  (+info)

Rho GTPase is activated by cytotoxic necrotizing factor 1 in peripheral blood T lymphocytes: potential cytotoxicity for intestinal epithelial cells. (7/124)

Some strains of Escherichia coli related to acute cystitis or colitis produce a toxin named cytotoxic necrotizing factor 1 (CNF-1). CNF-1 mediates its effects on epithelial cells or phagocytes via the permanent activation of small GTP-binding proteins, caused by the toxin-induced deamidation of Glu(63) of p21 Rho. The behavior of peripheral blood T lymphocytes during the acute phase of bacterial colitis has been poorly investigated. Our study was conducted to test whether (i) peripheral blood T lymphocytes can be activated by CNF-1 and (ii) CNF-1-activated T lymphocytes are cytotoxic against intestinal epithelial cells. Activation of T lymphocytes by CNF-1 was assessed by electrophoresis, flow cytometry, confocal microscopy, and electron microscopy studies. Assays for migration and adherence of CNF-1-treated T lymphocytes were performed in Transwell chambers with T84 intestinal epithelial cells grown on polycarbonate semipermeable filters. CNF-1 induced a decrease in the electrophoretic mobility of the GTP-binding protein Rho in treated T lymphocytes. CNF-1 provoked an increase in the content of actin stress fibers and pseudopodia in T lymphocytes. Several adherence molecules were clustered into cytoplasmic projections in CNF-1-treated T lymphocytes and adherence of such lymphocytes on the basolateral pole of T84 was increased, resulting in cytotoxicity toward epithelial cells. Such enhanced adherence in response to CNF-1 was dependent on p42-44(MAP) kinase activation of T lymphocytes. Taken together, these results suggest that CNF-1, by acting on T lymphocytes, may increase in an important fashion the virulence of certain strains of E. coli against the intestinal epithelia.  (+info)

Transmission of pseudorabies virus from immune-masked blood monocytes to endothelial cells. (8/124)

Pseudorabies virus (PRV) may cause abortion, even in the presence of vaccination-induced immunity. Blood monocytes are essential to transport the virus in these immune animals, including transport to the pregnant uterus. Infected monocytes express viral proteins on their cell surface. Specific antibodies recognize these proteins and should activate antibody-dependent cell lysis. Previous work showed that addition of PRV-specific polyclonal antibodies to PRV-infected monocytes induced internalization of viral cell surface proteins, protecting the cells from efficient antibody-dependent lysis in vitro (immune-masked monocytes). As a first step to reach the pregnant uterus, PRV has to cross the endothelial cell barrier of the maternal blood vessels. The current aim was to investigate in vitro whether immune-masked PRV-infected monocytes can transmit PRV in the presence of virus-neutralizing antibodies via adhesion and fusion of these monocytes with endothelial cells. Porcine blood monocytes, infected with a lacZ-carrying PRV strain, were incubated with PRV-specific antibodies to induce internalization. Then, cells were co-cultivated with endothelial cells for different periods of time. Only PRV-infected monocytes with internalized viral cell surface proteins adhered efficiently to endothelial cells. LacZ transmission to endothelial cells, as a measure for monocyte-endothelial cell fusion, could be detected after co-cultivation from 30 min onwards. Virus transmission was confirmed by the appearance of plaques. Adhesion of immune-masked PRV-infected monocytes to endothelial cells was mediated by cellular adhesion complex CD11b-CD18 and subsequent fusion was mediated by the virus. In conclusion, immune-masked PRV-infected monocytes can adhere and subsequently transmit virus to endothelial cells in the presence of PRV-neutralizing antibodies.  (+info)

Each subject will receive an initial conditioning dose of 0.7 mg/kg/week and then will continue treatment at a dose of 1.0 mg/kg/week. The treatment period will be 24 weeks divided into two phases: 1) double-blind for 12 weeks, and 2) open-label for 12 additional weeks, in which all subjects from the placebo group and those subjects from the Raptiva ® group with ≥ 50% of improvement will be allocated to extended treatment with Raptiva ® for 12 additional weeks while non-responders to Raptiva ® (improvement ≤ 50%) will be followed in an observational manner for 12 additional weeks without treatment ...
In October of 2008, the FDA issued whats generally known as a MedWatch alert thats designed to inform the public that a problem has been discovered and a change will be made to a product or medication. This has occurred with Raptiva because the FDA discovered that its been used to treat patients under the age of 18, and this approach has led to disastrous results in certain cases.. As a result, the FDA announced that Raptiva will now contain a stern warning on all of the labels of its containers that explicitly alert doctors who may use the drug to treat those patients under the age of 18, even though Raptiva has not been approved for use by those under 18. A labeling change is one of the more severe steps that the FDA can take, but falls short of a recall, which is the most drastic step. ...
Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrin ITGAL/ITGB2 is also a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL. Integrins ITGAM/ITGB2 and ITGAX/ITGB2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin ITGAX/ITGB2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin ITGAM/ITGB2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin ITGAM/ITGB2 is also a receptor for factor X. Integrin ITGAD/ITGB2 is a receptor for ICAM3 and VCAM1. Contributes to natural killer cell cytotoxicity. Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation. Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages. In association with alpha subunit ITGAM/CD11b, required ...
Efalizumab (Raptiva) lek je koji je korišten za tretiranje autoimunskih bolesti. Originalno je bio u prodaji za lečenje psorijaze. Efalizumab je humanizovan IgG1 kapa izotip monoklonalnog antitela koje se vezuje za ljudski CD11a. Efalizumab ima molekulsku težinu od oko 150 kilo Daltona i proizvodi se u CHO ćelijama.[2][3][4]. ...
Efalizumab - Get up-to-date information on Efalizumab side effects, uses, dosage, overdose, pregnancy, alcohol and more. Learn more about Efalizumab
Efalizumab is a man-made form of a protein similar to human antibodies. Efalizumab is made to target and destroy only certain cells in the body. This may help to protect healthy cells from damage.
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
This study examined the safety and potential efficacy of the monoclonal antibody efalizumab (Raptiva) for treating sight-threatening uveitis (eye inflammation). Efalizumab controls the activity of white blood cells called lymphocytes that cause inflammation. The drug is currently approved in the United States to treat patients with moderate to severe psoriasis.. Participants 18 and older with sight-threatening intermediate or posterior uveitis of at least 3 months duration, causing persistent macular edema in one or both eyes, were eligible for this study. The uveitis required treatment with at least 20 milligrams per day of prednisone, or the equivalent, or a combination of two or more anti-inflammatory treatments such as prednisone, methotrexate, cyclophosphamide, cyclosporine, etc.. Participants underwent the following tests and procedures:. ...
Poor preliminary Phase II results of Raptiva in psoriatic arthritis patients caused slight dips in Genentech Inc. and XOMA Ltd.s value. (BioWorld Today)
New Phytologist Editors Choice April 2015. In the latest issue of New Phytologist a Rapid report by Lee et al. describes recent insights into how the fungus that causes Septoria leaf blotch disease in wheat is able to hijack the hosts signalling
Although the precise pathogenesis is not known, vitiligo appears to be an autoimmune disease involving T cell-mediated melanocyte destruction. Efalizumab, a recombinant, humanized monoclonal antibody, targets T cells, the key mediators of the immunopathogenesis of psoriasis. Although a concomitant presentation of vitiligo with psoriasis is uncommon, several cases have been reported previously in the literature. A case of a patient with vitiligo and psoriasis who was treated with efalizumab to alleviate the symptoms of psoriasis is described. Over the course of the diseases, the patient had been treated unsuccessfully with numerous therapies. The patient initiated efalizumab with a 0.7 mg/kg conditioning dose and then continued on 1 mg/kg weekly. After 2 months of efalizumab therapy, the psoriasis symptoms were reduced, and the vitiligo had visibly improved in some areas. The patient has remained on efalizumab therapy with no evidence of an exacerbation of vitiligo. The management of acute flares is also
Integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide), also known as ITGAL, is a human gene which functions in the immune system. It is involved in cellular adhesion and costimulatory signaling. It is the target of the drug efalizumab. ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. CD11a is one of the two components, along with CD18, which form lymphocyte function-associated antigen-1. Efalizumab acts as an immunosuppressant by binding to CD11a but was ...
Integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide), also known as ITGAL, is a human gene which functions in the immune system. It is involved in cellular adhesion and costimulatory signaling. It is the target of the drug efalizumab.. ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling.[1]. CD11a is one of the two components, along with CD18, which form lymphocyte function-associated antigen-1.. Efalizumab acts as an immunosuppressant by binding to ...
Macrophages are among the infiltrate components of most malignant tumors. Tumor-associated macrophages (TAMs) may secrete a variety of humoral factors, which promote or inhibit tumor growth. In general, depending on their activation pathway, macrophages exhibit two different patterns of phenotype, M1 or M2. It is assumed that TAMs comprise pattern M2. In the malignant pleural effusion, macrophages are a frequent component of cytological evaluation. In this microenvironment, TAMs could be involved in the development of immunity. The phenotype of macrophages represented in malignant and non-malignant pleural effusions is unknown. In this study, macrophages were isolated from 38 pleural effusions (15 malignant and 23 non-malignant) and the expression of a variety of immune mediators and their receptors was assessed to determine the type of activation (M1 vs. M2). The expression of mRNA was analyzed for IL-1β, IL-4, IL-6, IL-10, IL-11, IL-18, TNFα, TGFβ1, IL1R1, IL1RAP, TLR2, TLR4, VLA4, CD62L, ...
TY - JOUR. T1 - Intercellular adhesion molecule inhibitors as potential therapy for refractory uveitic macular edema. AU - Wang, Jianmin. AU - Ibrahim, Mohamed. AU - Turkcuoglu, Peykan. AU - Hatef, Elham. AU - Khwaja, Afsheen. AU - Channa, Roomasa. AU - Do, Diana V. AU - Nguyen, Quan Dong. PY - 2010/10/1. Y1 - 2010/10/1. N2 - Purpose: To describe the bioactivity of an intercellular adhesion molecule inhibitor (efalizumab) in a patient with refractory uveitic macular edema. Methods: A 55-year-old man presented with idiopathic autoimmune uveitis and associated macular edema, which could not be controlled by regional and systemic corticosteroid and selected immunomodulatory therapy. Efalizumab was administered as subcutaneous injections. Results: After 37 weekly injections of efalizumab, the uveitic macular edema was successfully eliminated. Six months following discontinuation of efalizumab, there were no signs of recurrent inflammation. Conclusion: Further investigation of the role of ...
Clone REA378 recognizes the human CD11a antigen, a single-pass type I membrane protein also known as integrin α-L (ITGAL). Integrins are heterodimeric adhesion molecules comprised of α- and β-subunits that participate in immune cell interactions as well as in immune cell-extracellular matrix interactions. CD11a combines with CD18 (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 is a receptor for CD54 (ICAM-1), CD102 (ICAM-2), CD50 (ICAM-3), and CD242 (ICAM-4). It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T cell-mediated killing, and antibody dependent killing by granulocytes and monocytes. Interactions between LFA-1 and CD54 (ICAM-1) play a critical role in T cell-B cell collaboration. Additional information: Clone REA378 displays negligible binding to Fc receptors. - Danmark
Clone REA378 recognizes the human CD11a antigen, a single-pass type I membrane protein also known as integrin α-L (ITGAL). Integrins are heterodimeric adhesion molecules comprised of α- and β-subunits that participate in immune cell interactions as well as in immune cell-extracellular matrix interactions. CD11a combines with CD18 (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 is a receptor for CD54 (ICAM-1), CD102 (ICAM-2), CD50 (ICAM-3), and CD242 (ICAM-4). It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T cell-mediated killing, and antibody dependent killing by granulocytes and monocytes. Interactions between LFA-1 and CD54 (ICAM-1) play a critical role in T cell-B cell collaboration. Additional information: Clone REA378 displays negligible binding to Fc receptors. - Belgique
Therapy for the cutaneous disease is often difficult. Patients who present primarily with skin disease (amyopathic dermatomyositis) and those in whom the muscle component is controlled but who still have significant skin disease exist. The first-line of therapy is recognizing that the patient is photosensitive and advising the patient to avoid sun exposure and to use sun protective measures, including broad-spectrum sunscreens. Hydroxychloroquine and chloroquine have been beneficial in small open-label case studies. Methotrexate is also useful. Mycophenolate mofetil has been reported to be useful. IvIg not only benefited the muscle but also cleared the skin lesions in the patients in whom it was used. Rituximab has been used for skin disease, but the results are mixed. Efalizumab has been used and may have some benefit. Efalizumab (Raptiva), a drug indicated for psoriasis, is being withdrawn from the US market and will no longer be available after June 8, 2009, because of potential risk for ...
According to the study, the majority of Dermatologists reported an increase in their use of biologics for the treatment of psoriasis in the past year, particularly in patients with moderate psoriasis. While the increased use of biologics was seen across the board, certain physician types, particularly those newer to practice, seem to be more aggressive in their use of these agents. Amgen/Wyeths Enbrel continues to hold its position as market leader amongst the biologics, however, Abbotts Humira has increased its share significantly in the past year, and Dermatologists expect its growth to continue, primarily at the expense of Enbrel. Genentech/Xomas Raptiva and Astellas Amevive are rarely used with a collective share of less than 10%.. Despite the increased use of biologics, topical agents remain the most commonly prescribed therapy for psoriasis. Warner Chilcotts Taclonex, continues to hold its position as market leader among topical agents, although Galdermas Clobex is perceived to have ...
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Supplementary Materials Supplemental file 1 JVI. analyses of TMUV-infected chicken macrophages uncovered that web host antiviral innate immune system barriers had been the main goals of TMUV in macrophages. Regardless of the activation of main pattern reputation receptor signaling, the inductions of alpha interferon (IFN-) and IFN- had been obstructed by TMUV infections on transcription and translation amounts, respectively. In the meantime, TMUV inhibited web host redox replies by repressing the transcription of genes encoding NADPH oxidase subunits and marketing Nrf2-mediated antioxidant replies. The recovery of ITGAL either from the above-mentioned innate immune system barriers was enough to suppress TMUV infections. Collectively, we identify an important step of TMUV reveal and infection intensive subversion of host antiviral innate immune system responses. IMPORTANCE Mosquito-borne flaviviruses add a mixed band of pathogenic infections that trigger significant illnesses in human beings and ...
Tutor: Micaela Lewinson. Bioinformatics is an interdisciplinary area of study linking computational tools and databases to biology. Topics such as DNA sequence analysis, genome sequencing, expression of genes, 3D-structures of proteins are all considered parts of bioinformatics. In my Bioinformatics course (BIO 260) students are introduced to this exciting new interdisciplinary area spanning computational science and biology. This is a non-traditional biology course - it does not have a wet lab, but students spend a significant time in the computer lab using various databases (such as DNA sequence databases, protein structure databases) and software packages to solve problems in biology. One of the class projects is analysis and annotation of a previously unpublished, newly sequenced genome. Through this project students have an opportunity to use digital research to make a novel contribution to science! This exciting project is made possible through my participation in a multi-institution ...
Szántó, Sándor Zoltán and Bárdos, Tamás and Gál, István and Glant, Tibor T. and Mikecz, Katalin (2004) Enhanced neutrophil extravasation and rapid progression of proteoglycan-induced arthritis in TSG-6-knockout mice. ARTHRITIS AND RHEUMATISM, 50 (9). pp. 3012-3022. ISSN 0004-3591 ...
This live imaging approach provides a method to study cellular behavior during lung-branching morphogenesis and suggests the importance of a mechanism primarily based on oriented cell proliferation and mechanical forces in forming and shaping the developing lung airways.
Antoniou et al 24 reported that 2 of 72 patients with psoriasis treated with efalizumab had an HZ episode, with a crude incidence of HZ in 28 per 1000 patients. Diet is really difficult for most people and was something I really struggled with. Subsequently, the application time is titrated up to 20 to 30 minutes as tolerated. Use apple cider vinegar if the eczema herbs for psoriasis of the scalp red like pimples, wet, watery, or bleeding; or olive oil if the eczema has dry white skin peeling off.
Lifitegrast is a non-steroidal, small molecule, integrin antagonist that inhibits lymphocyte function-associated antigen-1 (LFA-1; CD11a/CD18), which is being
Nitrate administration has been proposed to enhance the detection of myocardial viability when performing myocardial perfusion imaging. In this study, we aimed to compare Tl-201 exercise-rest-reinjection protocol with rest isosorbide dinitrate (ISDN)-Tc99m MIBI study in the same population examined for the myocardial viability. ...
Integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide), also known as ITGAL, is ... antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)". Lu C, Takagi J, Springer TA (May 2001). " ... CD11a is one of the two components, along with CD18, which form lymphocyte function-associated antigen-1. Efalizumab acts as an ... CD11a+Antigen at the US National Library of Medicine Medical Subject Headings (MeSH) ITGAL Info with links in the Cell ...
... binds to the CD11a subunit of lymphocyte function-associated antigen 1 and acts as an immunosuppressant by ...
CD11a, CD18, CD54). Thus activated Vγ9Vδ2 T cells behave like APCs (γδ T-APC) and present antigens to αβ T cells. This leads to ... The antigens recognized by non-Vδ2 T cells expanded in the above infectious contexts have not been characterized, but the fact ... It is still not clear whether these non-peptidic antigens bind directly to the Vγ9/Vδ2 TCR or if a presenting element exists. ... However, none of the known antigen-presenting molecules like MHC class I and II or CD1 are required for γδ T cell activation ...
For example, LFA1 (CD11a/CD18) short representation of Lymphocyte Function-associated Antigen 1, also called αLβ2 integrin Mac1 ... CD11b/CD18) present on macrophages that is also called Macrophage-1 antigen (CR3) and αMβ2 integrin. CD11c/CD18 also called ...
... antigens, cd8 MeSH D23.050.301.264.035.111 - antigens, cd11 MeSH D23.050.301.264.035.111.024 - antigens, cd11a MeSH D23.050. ... antigens, cd8 MeSH D23.101.100.110.111 - antigens, cd11 MeSH D23.101.100.110.111.024 - antigens, cd11a MeSH D23.101.100.110. ... antigens, cd15 MeSH D23.101.100.900.131 - antigens, cd31 MeSH D23.101.100.920 - antigens, ly MeSH D23.101.100.930 - antigens, ... forssman antigen MeSH D23.050.285.018 - antigens, cd24 MeSH D23.050.285.025 - antigens, cd30 MeSH D23.050.285.040 - antigens, ...
Noncognate (not antigen specific) B cells play a significant role in the transport of antigens to FDCs. They capture immune ... Adhesion between FDCs and B cells is mediated by ICAM-1 (CD54)-LFA-1 (CD11a) and VCAM-VLA-4 molecules. Activated B-cells with ... Follicular DCs receptors CR1, CR2 and FcγRIIb trap antigen opsonized by complement or antibodies. These antigens are then taken ... To become selected as a future memory cell, GC B cells must bind the antigen presented on FDCs, otherwise they enter apoptosis ...
CD18+antigen at the US National Library of Medicine Medical Subject Headings (MeSH) ITGB2 Info with links in the Cell Migration ... The known binding partners of CD18 are CD11a, CD11b, CD11c and CD11d. Binding of CD18 and CD11 results in the formation of ... Huang C, Springer TA (August 1995). "A binding interface on the I domain of lymphocyte function-associated antigen-1 (LFA-1) ... LFA-1 is involved in adhesion and binding to antigen presenting cells through interactions with the surface protein ICAM-1 ...
CD11a / CD18 Macrophage-1 antigen (CR3) - Heterodimer: CD11b / CD18 Integrin alphaXbeta2 (CR4) - Heterodimer: CD11c / CD18 Very ... Antigen Antigenicity Immunogen Superantigen Allergen Hapten Epitope Linear Conformational Mimotope Tumor antigen Antigen- ... CD11a) AM (CD11b) AV AX (CD11c) Beta subunits B1 B2 B3 B4 B5 B6 B7 B8 Dimers Cytoadhesin receptor Integrin alpha6beta4 ... T cells Antigen receptor - T cell receptor (TCR) Subunits - [email protected] / [email protected] / [email protected] / [email protected] Co-receptors CD8 (CD8α / CD8β) CD4 ...
The alpha subunit is designated CD11a; and the beta subunit, unique to leukocytes, is beta-2 or CD18. The ICAM binding site is ... "Lymphocyte function-associated antigen 1 (LFA-1): a surface antigen distinct from Lyt-2,3 that participates in T lymphocyte- ... Lymphocyte function-associated antigen 1 (LFA-1) is an integrin found on lymphocytes and other leukocytes. LFA-1 plays a key ... The antigen that bound to the monoclonal antibodies was identified as an important molecule in cellular recognition processes. ...
It is a mouse monoclonal antibody directed against the alpha chain of the protein lymphocyte function-associated antigen 1 ... Whitcup SM, Chan CC, Kozhich AT, Magone MT (November 1999). "Blocking ICAM-1 (CD54) and LFA-1 (CD11a) inhibits experimental ... July 2002). "Anti-lymphocyte function-associated antigen-1 monoclonal antibody inhibits CD40 ligand-independent immune ...
Type 1 has a positive family history, starts before the age of 40, and is associated with the human leukocyte antigen, HLA-Cw6 ... Efalizumab is a monoclonal antibody that specifically targets the CD11a subunit of LFA-1. It also blocks the adhesion molecules ... Neutralization occurs when an antidrug antibody prevents a monoclonal antibody such as infliximab from binding antigen in a ... Specifically, neutralization occurs when the antidrug antibody binds to infliximab's antigen binding site instead of TNF-α. ...
It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by rhinovirus as a receptor for entry into ... Katz FE, Parkar M, Stanley K, Murray LJ, Clark EA, Greaves MF (Jan 1985). "Chromosome mapping of cell membrane antigens ... Huang C, Springer TA (Aug 1995). "A binding interface on the I domain of lymphocyte function-associated antigen-1 (LFA-1) ... ICAM-1 has been shown to interact with CD11a, EZR and CD18. GRCh38: Ensembl release 89: ENSG00000090339 - Ensembl, May 2017 ...
"Entrez Gene: ITGB3 integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)".. *^ May, K. E.; Villar, J.; Kirtley, S.; ... CD61+Antigens at the US National Library of Medicine Medical Subject Headings (MeSH) ...
... antigens[edit]. There are five (HNA 1-5) sets of neutrophil antigens recognized.[49] The three HNA-1 antigens (a-c) ... HNA-4 is located on the αM chain (CD11b) and HNA-5 is located on the αL integrin unit (CD11a). ... The HNA-3 antigen system has two antigens (3a and 3b) which are located on the seventh exon of the CLT2 gene (SLC44A2). The HNA ... and HNA-5 antigen systems each have two known antigens (a and b) and are located in the β2 integrin. ...
LFA-1 (CD11a+CD18). *Integrin alphaXbeta2 (CD11c+CD18). *Macrophage-1 antigen (CD11b+CD18) ...
LFA-1 (CD11a+CD18). *Integrin alphaXbeta2 (CD11c+CD18). *Macrophage-1 antigen (CD11b+CD18) ...
LFA-1 (CD11a+CD18). *Integrin alphaXbeta2 (CD11c+CD18). *Macrophage-1 antigen (CD11b+CD18) ... "Interaction of glycogen synthase kinase 3beta with the DF3/MUC1 carcinoma-associated antigen and beta-catenin". Molecular and ...
LFA-1 (CD11a+CD18). *Integrin alphaXbeta2 (CD11c+CD18). *Macrophage-1 antigen (CD11b+CD18) ... Primarily, the VCAM-1 protein is an endothelial ligand for VLA-4 (Very Late Antigen-4 or integrin α4β1) of the β1 subfamily of ...
LFA-1 (CD11a+CD18). *Integrin alphaXbeta2 (CD11c+CD18). *Macrophage-1 antigen (CD11b+CD18) ... In humans, the CD44 antigen is encoded by the CD44 gene on Chromosome 11.[5] CD44 has been referred to as HCAM (homing cell ... The CD44 antigen is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. ... Indian blood group system at BGMUT Blood Group Antigen Gene Mutation Database at NCBI, NIH ...
... antigens, cd11a MeSH D12.776.543.750.705.408.100.150 - antigens, cd11b MeSH D12.776.543.750.705.408.100.200 - antigens, cd11c ... antigen, b-cell MeSH D12.776.543.750.705.816.821.500 - antigens, cd79 MeSH D12.776.543.750.705.816.824 - receptors, antigen, t- ... antigens, cd22 MeSH D12.776.543.550.200.124 - antigens, cd24 MeSH D12.776.543.550.200.131 - antigens, cd31 MeSH D12.776.543.550 ... antigens, cd27 MeSH D12.776.543.750.705.852.760.072 - antigens, cd30 MeSH D12.776.543.750.705.852.760.097 - antigens, cd40 MeSH ...
... and characterization of its interaction with the leukocyte integrin CD11a/CD18". Eur. J. Immunol. 30 (3): 810-8. doi:10.1002/ ... chromosomal localization of the human telencephalin and its distinctive interaction with lymphocyte function-associated antigen ...
CD11a. *CD11b. Bibliografía[editar , editar a fonte]. *. Stewart M, Thiel M, Hogg N (1996). "Leukocyte integrins.". Curr. Opin ... Human CD Antigen Chart. *ITGAX Info with links in the Cell Migration Gateway ...
CD3 (Muromonab-CD3, Oteliksizumab, Teplizumab, Visilizumab) • CD4 (Klenoliksimab, Keliksimab, Zanolimumab) • CD11a (Efalizumab) ... su naspramni jedan drugom i formiraju antigen-vezujuće mesto, tako da molekul antitela sadrži dva antigen-vezujuća mesta. ... Funkcionira uglavnom kao antigen receptor na B-limfocitima koji nisu bili izloženi antigenima. Dokazano je da aktivira bazofile ... i spremnim da odgovore na antigen. Aktivacija B ćelija prati angažman ćelijski vezanih molekula antitijela sa antigenom, ...
Goodpasture-antigen-binding protein kinase (EC 2.7.11.9). *-. IκB kinase (EC 2.7.11.10). *CHUK ...
Past this period CD3 blocks the TCR-antigen binding and causes conformational change or the removal of the entire TCR3/CD3 ... The IL-2a (CD25, T-cell activation antigen, TAC) is expressed only by the already-activated T lymphocytes. Therefore, it is of ... The antilymphocyte (ALG]) and antithymocyte antigens (ATG) are being used. They are part of the steroid-resistant acute ... Monoclonal antibodies are directed towards exactly defined antigens. Therefore, they cause fewer side-effects. Especially ...
"Guiding the selection of human antibodies from phage display repertoires to a single epitope of an antigen". Bio/Technology. 12 ... drug were found by guiding the selection of human antibodies from phage display repertoires to a single epitope of an antigen ...
CD3 (Muromonab-CD3, Oteliksizumab, Teplizumab, Visilizumab) • CD4 (Klenoliksimab, Keliksimab, Zanolimumab) • CD11a (Efalizumab) ... ćelije bile identifikovane kao najpotentniji proizvođači tipa I interferona u odgovoru na antigen, i bile su nazvani prirodne ...
CD3 (Muromonab-CD3, Oteliksizumab, Teplizumab, Visilizumab) • CD4 (Klenoliksimab, Keliksimab, Zanolimumab) • CD11a (Efalizumab) ... Induction of Potent and Long-Lasting T-Cell Responses against Cancer Antigens". Cancer Research 62: 1477-1480. ... "A divalent major histocompatibility complex/IgG1 fusion protein induces antigen-specific T cell activation in vitro and in ...
CD3 (Muromonab-CD3, Oteliksizumab, Teplizumab, Visilizumab) • CD4 (Klenoliksimab, Keliksimab, Zanolimumab) • CD11a (Efalizumab ... Nakon presađivanja (transplantacije) organa, telo skoro uvek „odbaci" novi organ usled razlike ljudskih leukocit antigen ...
CD3 (Muromonab-CD3, Oteliksizumab, Teplizumab, Visilizumab) • CD4 (Klenoliksimab, Keliksimab, Zanolimumab) • CD11a (Efalizumab ... ćelije bile identifikovane kao najpotentniji proizvođači tipa I interferona u odgovoru na antigen, i bile su nazvani prirodne ...
Past this period CD3 blocks the TCR-antigen binding and causes conformational change or the removal of the entire TCR3/CD3 ... The IL-2a (CD25, T-cell activation antigen, TAC) is expressed only by the already-activated T lymphocytes. Therefore, it is of ... The antilymphocyte (ALG) and antithymocyte antigens (ATG) are being used. They are part of the steroid-resistant acute ... Monoclonal antibodies are directed towards exactly defined antigens. Therefore, they cause fewer side-effects. Especially ...
Antigens, cd11a explanation free. What is Antigens, cd11a? Meaning of Antigens, cd11a medical term. What does Antigens, cd11a ... Looking for online definition of Antigens, cd11a in the Medical Dictionary? ... CD11a. (redirected from Antigens, cd11a) CD11a. a type I transmembrane protein found on lymphocytes, neutrophils, monocytes, ... Antigens, cd11a , definition of Antigens, cd11a by Medical dictionary https://medical-dictionary.thefreedictionary.com/Antigens ...
Shop a large selection of products and learn more about CD11a Mouse anti-Human, Biotin, Clone: OFYS, Invitrogen 100 Tests; ... CD11 antigen-like family member A, LFA-1 alpha, LFA-1A, antigen CD11A p180, lymphocyte function-associated antigen 1 alpha ... CD11a (LFA-1 alpha, ITGAL) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 ... CD11a is implicated in activation of LFA-1 complex. CD11a plays a central role in leukocyte intercellular adhesion through ...
Antigen Expression CD11a +; CD19 +; CD20 +; CD38 -; CD49e +. Receptor Expression growth hormone receptor ...
Antigen Expression CD11a +; CD19 +; CD20 +; CD28 +; CD38 -; CD49e +. Comments The cells are positive for Epstein-Barr nuclear ... Generation of human monoclonal antibodies reactive with cellular antigens. Proc. Natl. Acad. Sci. USA 80: 2026-2030, 1983. ... Generation of human monoclonal antibodies reactive with cellular antigens. Proc. Natl. Acad. Sci. USA 80: 2026-2030, 1983. ...
This study reports the sequencing of the wild boar beta2-integrin CD11a and CD18 cDNAs. Predicted CD11a and CD18 subunits share ... Besides these strong overall similarities, wild boar and domestic pig LFA-1 differ by 2 (CD18) and 1 or 3 (CD11a) substitutions ... of which one is located in the crucial I-domain (CD11a, E168D). As most wild boars are seropositive to the RTX toxin-producing ... CD11a/CD18, alphaLbeta2), expressed on all leukocytes, is essential for many adhesive functions of the immune system. ...
CD11a is the a chain of the leukocyte function associated antigen-1 LFA-1a). Background Chronic obstructive pulmonary disease ( ... CD11a is the a chain of the leukocyte function associated antigen-1 LFA-1a), a member of the integrin subfamily, and ... CD11a is the a chain of the leukocyte function associated antigen-1 (LFA-1a), and is expressed on all leukocytes including T ... Mouse monoclonal to CD11a.4A122 reacts with CD11a, NK and T-cell cytolysis, such as lymphocyte adhesion ...
CD11a is a subunit of LFA-1, a cell surface molecule involved in T cell mediated... ... a human anti-CD11a antibody, were investigated in human subjects with psoriasis. ... The human lymphocyte function-associated (HLFA) antigen and a related macrophage differentiation antigen (HMac-1): Functional ... The pharmacokinetics of hu1124, a human anti-CD11a antibody, were investigated in human subjects with psoriasis. CD11a is a ...
CD11a/CD18; αL:β2) (T cells) and (ii) the interactions between lymphocyte function-associated antigen 3 (LFA-3; CD58) (DC) and ... Sensitization phase - antigen processing and presentation. The initial step of every specific immune reaction to an antigen (Ag ... Cutaneous lymphocyte antigen is a specialized form of PSGL-1 expressed on skin-homing T cells. Nature 1997: 389: 978-981. * ... Cutting edge: CD1a+ antigen-presenting cells in human dermis respond rapidly to CCR7 ligands. J Immunol 2006: 176: 5730-5734. * ...
The alpha subunit is designated CD11a; and the beta subunit, unique to leukocytes, is beta-2 or CD18. The ICAM binding site is ... "Lymphocyte function-associated antigen 1 (LFA-1): a surface antigen distinct from Lyt-2,3 that participates in T lymphocyte- ... Lymphocyte function-associated antigen 1 (LFA-1) is an integrin found on lymphocytes and other leukocytes. LFA-1 plays a key ... The antigen that bound to the monoclonal antibodies was identified as an important molecule in cellular recognition processes. ...
Name Handelsname Typ Zielstruktur Anwendungsgebiet . Efalizumab Raptiva humanisiert CD11a-Antigen Psoriasis . Golimumab Simponi ... CD11a-Antigen Psoriasis . Golimumab Simponi human TNF-α (Tumor Necrosis Factor α) Colitis ulcerosa, Rheumatoide Arthritis, ...
Invitrogen Anti-CD11a (LFA-1alpha) Monoclonal (M17/4), eBioscience , Catalog # 63-0111-82. Tested in Flow Cytometry (Flow) ... Protein Aliases: alpha L integrin; antigen CD11A p180; CD11 antigen-like family member A; integrin alpha-L; integrin alphaL; ... CD11a (LFA-1 alpha, ITGAL) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 ... T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell cell adhesion ...
CD11A (LFA1) I-domain complexed with 7A-[(4-cyanophenyl)methyl]-6-(3,5-dichlorophenyl)-5-oxo-2,3,5,7A-tetrahydro-1H-pyrrolo[1,2 ... A new class of lymphocyte function-associated antigen-1 (LFA-1) antagonists is described. Elaboration of the 2,3-dihydro-1H- ... CD11A (LFA1) I-domain complexed with 7A-[(4-cyanophenyl)methyl]-6-(3,5-dichlorophenyl)-5-oxo-2,3,5,7A-tetrahydro-1H-pyrrolo[1,2 ... A new class of lymphocyte function-associated antigen-1 (LFA-1) antagonists is described. Elaboration of the 2,3-dihydro-1H- ...
CD11a combines with CD18 (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on ... Clone REA378 recognizes the human CD11a antigen, a single-pass type I membrane protein also known as integrin α-L (ITGAL). ... CD11a combines with CD18 (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on ... Clone REA378 recognizes the human CD11a antigen, a single-pass type I membrane protein also known as integrin α-L (ITGAL). ...
... a tumor associated antigen such as HER2, HER3 or HER4 receptor; antibodies against various protein antigens like CD proteins ... anti-CD11a, anti-CD18 or anti-CD11b antibodies); growth factors such as VEGF; superoxide dismutase; factor; anti-clotting ... blood group antigens; flk2/flt3 receptor; obesity (OB) receptor; insulin A-chain; insulin B-chain; interleukins (ILs), e.g., IL ... viral antigen such as, for example, a portion of the AIDS envelope, gp120; transport proteins; homing receptors; addressins; ...
CD11a, CD11c, and CD18 gene polymorphisms and susceptibility to Behçets disease in Koreans. Tissue Antigens. 2014 Oct. 84(4): ... Skin hypersensitivity to streptococcal antigens and the induction of systemic symptoms by the antigens in Behçets disease--a ...
Integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide), also known as ITGAL, is ... antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)". Lu C, Takagi J, Springer TA (May 2001). " ... CD11a is one of the two components, along with CD18, which form lymphocyte function-associated antigen-1. Efalizumab acts as an ... CD11a+Antigen at the US National Library of Medicine Medical Subject Headings (MeSH) ITGAL Info with links in the Cell ...
Expression of adhesion molecules L-selectin (A), α4-integrin (B), CD11a (C), and intercellular adhesion molecule (ICAM)-1 (D) ... This process enables naive cells to increase the frequency of encounters with cognate antigens (9, 26). These lymphocytes have ... Effect of monoclonal antibodies against CD11a (WT-3), α4-integrin (MRα4-1), L-selectin (HRL3), or ICAM-1 (1A-29) and GdCl3 ... 4, all of the adhesion molecule-specific antibodies tested (anti-L-selectin, anti-CD11a, and anti-α4-integrin) did not alter ...
Integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide), also known as ITGAL, is ... Integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide). ... "Entrez Gene: ITGAL integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)". http ... Entrez Gene: ITGAL integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide). ...
... antigen CD11A (p180)... GraphId=d01, ,Graphics Color=000000 CenterX=13100.0 CenterY=9000.0 Width=900.0 Height=300.0 ... antigen CD18 (p95) l... GraphId=e20, ,Graphics Color=000000 CenterX=14550.0 CenterY=8700.0 Width=900.0 Height=300.0 ... antigen CD49C alpha... GraphId=b06, ,Graphics Color=000000 CenterX=10800.0 CenterY=9000.0 Width=900.0 Height=300.0 ... antigen CD49D alpha... GraphId=a0e, ,Graphics Color=000000 CenterX=10800.0 CenterY=9300.0 Width=900.0 Height=300.0 ...
... antigen CD11A (p180)... /, ,Graphics CenterX=873.3333333333334 CenterY=600.0 Width=60.0 Height=20.0 ZOrder=32768 ... antigen CD18 (p95) l... /, ,Graphics CenterX=970.0 CenterY=580.0 Width=60.0 Height=20.0 ZOrder=32768 FontSize=10 ... antigen CD49C alpha... /, ,Graphics CenterX=720.0 CenterY=600.0 Width=60.0 Height=20.0 ZOrder=32768 FontSize=10 ... antigen CD49D alpha... /, ,Graphics CenterX=720.0 CenterY=620.0 Width=60.0 Height=20.0 ZOrder=32768 FontSize=10 ...
... purified antigen or cells or cellular extracts carrying the antigen) into an animal (e.g., a mouse) so as to elicit a desired ... Chikao Morimoto (Dana-Farber Cancer Inst.); and 10F12 (CD18) and 2F12 (CD11a) mAb provided by Dr. Jerry Ritz (Dana-Farber ... "B-cell antigens: CD22." In Leukocyte Typing IV. White Cell Differentiation Antigens, Knapp et al., eds., Oxford University ... "B-cell antigens: CDw75". In "Leukocyte Typing IV. White Cell Differentiation Antigens". Knapp et al., eds. Oxford University ...
Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte- ... Ab28093 reacts with an epitope located in the region encoded by aa 534-546 in cysteine rich repeat 3 of the CD18 antigen. ...
The first phase is rapid (2 to 4 h) and allows the detection of Gag antigens in 5 to 10% of target cells. This time frame is ... CD11a (TS1/22; ATCC), CD18 (TS1/18; ATCC), and ICAM-1 and ICAM-3 (F10.2 and CBRR-IC3/1, respectively; Fifth Workshop on Human ... After a few days of culturing, when about 70% expressed Gag antigens, cells were cocultivated at a 1/1.5 ratio with recipient ... Two days later, when about 25% of the cells expressed HIV Gag antigens, further viral replication was stopped with the reverse ...
Exbio - Research products - Antibodies - CD and related antigens - Anti-Hu CD11a FITC ... The antibody MEM-25 reacts with an extracellular epitope of CD11a (alpha subunit of human LFA-1), a 170-180 kDa type I ... Fujii T, Takada-Takatori Y, Kawashima K: Roles played by lymphocyte function-associated antigen-1 in the regulation of ... Bazil V, Stefanova I, Hilgert I, Kristofova H, Vanek S, Horejsi V.: Monoclonal antibodies against human leucocyte antigens. IV ...
Exbio - Research products - Antibodies - CD and related antigens - Anti-Hu CD11a Purified ... Separation of human CD11a positive lymphocytes (red-filled) from CD11a negative blood debris (black-dashed) in flow cytometry ... The antibody MEM-83 reacts with an extracellular epitope of CD11a (alpha subunit of human LFA-1), a 170-180 kDa type I ... Fujii T, Takada-Takatori Y, Kawashima K: Roles played by lymphocyte function-associated antigen-1 in the regulation of ...
Population pharmacokinetics and pharmacodynamics of the anti-CD11a antibody hu1124 in human subjects with psoriasis. J ... If some reported covariates, such as the circulating antigen level or tumour size, are likely to be correlated to antigen mass ... In some cases, antigen targets exist in different forms, notably in the circulation and expressed at the cell surface. The ... Influence of FCGR3A-158 V/F genotype and baseline CD20 antigen count on target-mediated elimination of rituximab in patients ...
The leukocyte function-associated antigen 1 (LFA-1; CD11a/CD18) is one of the integrins (β2 integrins) exclusively expressed on ... 1993) Role of intracellular Ca2+ levels in the regulation of CD11a/CD18 mediated cell adhesion. Cell Adhes. Commun. 1:21-32. ... 1999) Affinity modulation of very late antigen-5 through phosphatidylinositol 3-kinase in mast cells. J. Immunol. 162:2850-2857 ... 1985) IL-3-dependent mouse clones that express B-220 surface antigen, contain Ig genes in germ-line configuration, and generate ...
CD11a and CD49d enhance the detection of antigen-specific T cells following human vaccination. ... Recovery of antigen-specific T cell responses from dogs infected with Leishmania (L.) infantum by use of vaccine associated TLR ... Identification of Novel Antigens Recognized by Serum Antibodies in Bovine Tuberculosis.. Lyashchenko KP, Grandison A, Keskinen ... Not All Antigens Are Created Equally: Progress, Challenges, and Lessons Associated with Developing a Vaccine for Leishmaniasis. ...
Immunologic tolerance is a state of immune unresponsiveness specific to a particular antigen or set of antigens induced by ... previous exposure to that antigen or set. Tolerance is generally accepted to be an active process and, in essence, a learning ... Antithymocyte globulin contains cytotoxic antibodies that bind to CD2, CD3, CD4, CD8, CD11a, CD18, CD25, CD44, CD45 and HLA ... Sequestration of antigens into privileged sites. Some antigens are sequestered into privileged sites away from the immune ...
integrin, alpha E (antigen CD103, human m.... 3683. 3683. ITGAL. integrin, alpha L (antigen CD11A (p180), .... ...
  • CD11a (LFA-1 alpha, ITGAL) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. (fishersci.com)
  • The most predominant beta 2-integrin lymphocyte function-associated antigen-1 (LFA-1, CD11a/CD18, alpha L beta 2), expressed on all leukocytes, is essential for many adhesive functions of the immune system. (biomedcentral.com)
  • This study reports the sequencing of the wild boar beta 2-integrin CD11a and CD18 cDNAs. (biomedcentral.com)
  • Predicted CD11a and CD18 subunits share all the main structural characteristics of their mammalian homologues, with a larger interspecies conservation for the CD18 than the CD11a. (biomedcentral.com)
  • Besides these strong overall similarities, wild boar and domestic pig LFA-1 differ by 2 (CD18) and 1 or 3 (CD11a) substitutions, of which one is located in the crucial I-domain (CD11a, E168D). (biomedcentral.com)
  • LFA-1, formed by non-covalent association of CD11a with CD18 (integrin beta 2), serves as an important adhesion molecule involved in lymphocyte and granulocyte function. (thermofisher.com)
  • CD11a combines with CD18 (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. (miltenyibiotec.com)
  • CD11a is one of the two components, along with CD18, which form lymphocyte function-associated antigen-1. (wikipedia.org)
  • Ab28093 reacts with an epitope located in the region encoded by aa 534-546 in cysteine rich repeat 3 of the CD18 antigen. (abcam.com)
  • CD11a/CD18) is one of the integrins (β2 integrins) exclusively expressed on leukocytes, and its counterligands are the intercellular adhesion molecules 1, 2, and 3 (ICAM-1, -2, and -3) ( 13 , 35 , 59 ). (asm.org)
  • ICAM1 binds to integrins such as CD11a / CD18, or CD11b / CD18. (prospecbio.com)
  • Lymphocyte function-associated antigen 1 is a heterodimer integrin CD11a (integrin alpha L, p189) and CD18 (integrin beta-2). (brainscape.com)
  • CD18 non-covalently associates with CD11a, CD11b or CD11c. (biolegend.com)
  • 2 In addition, fluvastatin inhibits the expression of lymphocyte function-associated antigen (CD11a/CD18) and ICAM-1 on human monocytic cell lines. (ahajournals.org)
  • CD11a is the integrin alpha L chain that forms with CD18 the heterodimer molecule Lymphocyte Function-associated Antigen-1 (LFA-1). (peprotech.com)
  • 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). (ptglab.com)
  • CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160). (ptglab.com)
  • CD11a/CD18) in cytokine-triggered firm leukocyte adhesion to arterial endothelium in vivo. (lu.se)
  • Here we describe the identification of CD11b, the α subunit of the αM/β2 integrin (CD11b/CD18), macrophage-1 antigen, or complement receptor 3, as a cellular receptor for leukocidin A/B (LukAB), an important toxin that contributes to S. aureus killing of human neutrophils. (pnas.org)
  • Lymphocyte and monocyte ligands, L-selectin, sialated glycoproteins, lymphocyte function-associated antigen 1 (LFA-1) (CD11a, CD18), and Mac1 (CD11b, CD18) bind to the EC rec and modulate the migration of these cells. (medscape.com)
  • Lymphocyte function-associated antigen 1 (LFA-1) consists of an alpha-chain (CD11a) and beta-chain (CD18). (allergycases.org)
  • Macrophage-1 antigen, MAC-1 (integrin alphaMbeta2) is a complement receptor (CR3) consisting of CD11b and CD18. (allergycases.org)
  • CD11a/CD18) when migrating in response to chemoattractants either across the vasculature into lymph nodes or across stimulated vessels associated with infected tissue. (rupress.org)
  • Interactions between cytotoxic lymphocytes and their targets require the T cell antigen receptor (TCR) and the integrin lymphocyte function-associated molecule-1 (LFA-1, CD11a/CD18). (sciencemag.org)
  • The CD11/CD18 adhesion molecules consist of three heterodimers sharing a common beta subunit (CD18) with a distinct alpha subunit (CD11a, CD11b, CD11c). (fishersci.com)
  • The intercellular adhesion molecules ICAM-1, 2 and 3 are, like VCAM-1, members of the immunoglobulin superfamily (IgSF), but they are recognized by an I domain-containing integrin, lymphocyte-function-associated antigen 1 (LFA-1, or CD11a/CD18). (rcsb.org)
  • Antibodies to CD11a/CD18 (leukocyte function-associated antigen-1 [LFA-1]) present in ATG induced a dose-dependent down-modulation of cell surface expression of this beta2 integrin on lymphocytes, monocytes, and neutrophils. (nih.gov)
  • In humans, lymphocyte adhesion to cells is mediated by the protein heterodimer CD11a/CD18 (Leu-CAMa, LFA-1) and its ligand CD54 (ICAM-1). (ihcworld.com)
  • LKT was first reported to bind to the β 2 integrin CD18/CD11a, also known as leukocyte functional antigen 1 (LFA-1) ( 1 , 24 , 28 ). (asm.org)
  • There is increasing experimental evidence that blocking the interaction between ICAM-1, which is expressed on endothelium, and integrins such as lymphocyte function-associated antigen-1 (CD11a/CD18) and macrophage antigen-1 (complement receptor 3, CD11b/CD18), which are expressed on the surface of leukocytes, prevents not only inflammation of vessel walls but also chronic vasospasm. (thejns.org)
  • The cell adhesion molecules involved in this process are lymphocyte function-associated antigen-1 (CD11a/CD18) and macrophage antigen-1 (CD11b/CD18), which are present on neutrophils/macrophages, and intercellular adhesion molecule-1 (CD54), which is present in endothelial cells. (thejns.org)
  • For example, humanized anti- CD11a antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity. (fishersci.com)
  • i) interaction between lymphocytes, (ii) interaction between T-cells and antigen presenting cells, (iii) adhesion of naïve lymphocytes to post-capillary high endothelial venules of secondary lymphoid tissues, (iv) adhesion of leukocytes to activated endothelium at sites of inflammation for extravasation, (v) control of cell differentiation and proliferation, and (vi) antibody-dependent killing by natural killer cells and granulocytes. (biomedcentral.com)
  • The pharmacokinetics of hu1124, a human anti-CD11a antibody, were investigated in human subjects with psoriasis. (springer.com)
  • Humanization of an anti-lymphocyte function-associated antigen (LFA)-1 monoclonal antibody and reengineering of the humanized antibody for binding to rhesus LFA-1. (springer.com)
  • The following product was used in this experiment: CD11a (LFA-1alpha) Monoclonal Antibody (M17/4), Super Bright 600, eBioscience from Thermo Fisher Scientific, catalog # 63-0111-82, RRID AB_2762656. (thermofisher.com)
  • Description: The M17/4 monoclonal antibody reacts with mouse CD11a, the 180 kDa integrin alpha L, also known as the lymphocyte function associated antigen-1 (LFA-1) alpha chain. (thermofisher.com)
  • Amplification of Human B Cell Activation by a Monoclonal Antibody to the B Cell-Specific Antigen CD22, Bp 130/140," J. Immunol. (freepatentsonline.com)
  • Antibodies exhibit complex pharmacokinetic properties, notably owing to the influence of antigen mass, i.e. the amount of antigenic targets to which the monoclonal antibody binds specifically. (springer.com)
  • To maximise therapeutic efficacy, sufficient antibody doses should be administered to ensure the saturation of antigen targets by therapeutic antibodies in all patients. (springer.com)
  • The antibody MEM-25 reacts with an extracellular epitope of CD11a (alpha subunit of human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. (exbio.cz)
  • Flow cytometry surface staining pattern of human peripheral blood cells stained using anti-human CD11a (MEM-83) purified antibody (concentration in sample 1 μg/ml) GAM APC. (exbio.cz)
  • Separation of human CD11a positive lymphocytes (red-filled) from CD11a negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD11a (MEM-83) purified antibody (concentration in sample 1 μg/ml) GAM APC. (exbio.cz)
  • Dr. Richard Trauger, CytoDyn's Chief Scientific Officer, will present a poster titled "Influence of anti-LFA-1 on FIV infection ex vivo and in vivo," describing new preliminary findings regarding CytoFeline™, a feline reactive monoclonal antibody targeting the cell antigen CD11a under development by CytoDyn for the treatment of feline immunodeficiency virus. (cnbc.com)
  • The Company's proprietary drug candidate Cytolin(R) is a monoclonal antibody that binds to CD11a, a cellular antigen that is a component of the cellular adhesion molecule LFA-1. (cnbc.com)
  • The HI111 monoclonal antibody specifically reacts with human CD11a, a 180kDA glycoprotein. (peprotech.com)
  • 1X10^6 human peripheral blood lymphocytes were surface stained with 5 ul CoraLite®647-conjugated Anti-Human CD11a (CL647-65194, Clone: TS2/4) (red) or isotype control antibody (blue). (ptglab.com)
  • To evaluate the safety and efficacy of treating macular edema, secondary to noninfectious uveitis, with a humanized anti-CD11a antibody. (arvojournals.org)
  • Anti-CD11a antibody treatments resulted in an increase in the CD56 bright regulatory NK cell population in the peripheral blood of the patients. (arvojournals.org)
  • Genentech Inc., San Francisco, CA) is a humanized form of a murine IgG1 antibody directed against CD11a, the α-subunit of lymphocyte function-associated antigen-1 (LFA-1). (arvojournals.org)
  • Efalizumab (Raptiva), used to treat psoriasis, is a recombinant humanized monoclonal antibody that binds to CD11a and acts as an immunosuppressant. (allergycases.org)
  • Applications Tested: This 118/A5 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval. (fishersci.com)
  • Each antibody is crafted with care according to rigorous protocols for immunogen design and preparation, presentation to host animal, and high-affinity purification against the antigen. (abgent.com)
  • A monoclonal anti-prostate specific membrane antigen antibody used in imaging kits to target radioactive agents to malignant prostate tissue. (drugbank.ca)
  • A monoclonal anti-CD11a antibody used to treat moderate to severe plaque psoriasis. (drugbank.ca)
  • In addition, treatment with hu1124 caused a rapid reduction in the level of CD11a expression on CD3-positive lymphocytes (T cells) to about 25% of pretreatment levels. (springer.com)
  • Lymphocyte function-associated antigen 1 (LFA-1) is an integrin found on lymphocytes and other leukocytes. (wikipedia.org)
  • HD39 (B3), A B Lineage-Restricted Antigen Whose Cell Surface Expression is Limited to Resting and Activated Human B Lymphocytes," J. Immunol. (freepatentsonline.com)
  • Mycobacterium leprae Recombinant Antigen Induces High Expression of Multifunction T Lymphocytes and Is Promising as a Specific Vaccine for Leprosy. (nih.gov)
  • The persistence of antigen will lead to loss of co-stimulatory molecules, telomere shortening and defective IL-2 production, changes that define the state replicative senescence in T lymphocytes. (nih.gov)
  • In T lymphocytes, antigen recognition triggers signal transduction by clustering T cell receptor (TCR)/CD3 multiprotein complexes. (sciencemag.org)
  • We have investigated the distribution, activity, and cytoskeletal linkage of the integrin lymphocyte function associated antigen-1 (LFA-1) on human T lymphocytes migrating on endothelial cells and on ligand intercellular adhesion molecule-1 (ICAM-1). (rupress.org)
  • CD11a is a leukocyte marker that is expressed in B and T lymphocytes, macrophages, monocytes, neutrophils, basophils and eosinophils. (fishersci.com)
  • The four alpha subunits are: CD11a (αL) (all leukocytes), CD11b (αM) (granulocytes, monocytes, some macrophages), CD11c (αX) (granulocytes, monocytes, dendritic antigen presenting cells), CD11d (αD) (CD8 T-cell subpopulation including large granular lymphocytes, macrophages and gamma-delta T cells in splenic red pulp). (beckman.com)
  • Although CD11a and CD11b share structural similarities, are both located in a gene cluster on human chromosome 16, and function as ligands for ICAM-1 (CD54), these molecules clearly have differences in expression and unique functions that are not overlapping. (jimmunol.org)
  • In particular, CD11 is composed of CD11a, CD11b and CD11c. (fishersci.com)
  • Expression of the adhesion molecules CD11a, CD11b, CD11c, very late antigen-4 (VLA-4), and L-selectin, as assessed by flow cytometry, was increased in the stimulated U937 cells, and AT resulted in significant reduction in the expression of CD11b and VLA-4. (ahajournals.org)
  • 3 These adhesion molecules include E-selectin, intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1) on ECs and members of the β-2 integrin family (CD11a, CD11b, and CD11c/18) and the β-1 integrin VLA-4 (very late antigen-4 [CD49d/29]) on monocytes. (ahajournals.org)
  • Clone REA378 recognizes the human CD11a antigen, a single-pass type I membrane protein also known as integrin α-L (ITGAL). (miltenyibiotec.com)
  • Integrin, alpha L (antigen CD11A, lymphocyte function-associated antigen 1) is also known as ITGAL or CD11a. (allergycases.org)
  • Blocking of CD11a function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. (fishersci.com)
  • Generation of human monoclonal antibodies reactive with cellular antigens. (atcc.org)
  • The human lymphocyte function-associated (HLFA) antigen and a related macrophage differentiation antigen (HMac-1): Functional effects of subunit-specific monoclonal antibodies. (springer.com)
  • The antigen that bound to the monoclonal antibodies was identified as an important molecule in cellular recognition processes. (wikipedia.org)
  • This review focuses on the influence of antigen mass on the pharmacokinetics of therapeutic antibodies quantified by pharmacokinetic modelling in humans. (springer.com)
  • Dr. Elder's work, which has been previously reported, demonstrated that feline reactive antibodies to a normal cell protein known as CD11a could inhibit FIV infection of susceptible target cells. (cnbc.com)
  • Anti-interleukin (IL)-1α/β and anti-tumor necrosis factor-α antibodies, or anti-lymphocyte function-associated antigen-1 and very late antigen-4 antibodies, had little or no inhibitory effects on MCP-1 secretion by cocultures. (ahajournals.org)
  • The variety of isoforms include monomeric or polymeric forms, and transmembrane forms (B-CELL ANTIGEN RECEPTORS) or secreted forms (ANTIBODIES). (drugbank.ca)
  • Efalizumab acts as an immunosuppressant by binding to CD11a but was withdrawn in 2009 because it was associated with severe side effects. (wikipedia.org)
  • Efalizumab acts as an immunosuppressant by binding to CD11a. (thefullwiki.org)
  • 7 , 8 In vitro studies have shown that by binding to CD11a, efalizumab can inhibit T-cell activation, T-cell trafficking, and T-cell adhesion without depleting the T cells. (arvojournals.org)
  • A new class of lymphocyte function-associated antigen-1 (LFA-1) antagonists is described. (rcsb.org)
  • Fujii T, Takada-Takatori Y, Kawashima K: Roles played by lymphocyte function-associated antigen-1 in the regulation of lymphocytic cholinergic activity. (exbio.cz)
  • T cells overexpressing lymphocyte function-associated antigen 1 become autoreactive and cause a lupuslike disease in syngeneic mice. (abnova.com)
  • Previous studies demonstrate that combined anti-lymphocyte function-associated antigen-1 (LFA-1) plus anti-CD154 therapy results in a robust form of islet allograft tolerance not observed with either individual monotherapy. (diabetesjournals.org)
  • Our data demonstrate that CD40 triggering on XG2 cells induced a myeloma cell growth arrest mediated by lymphocyte function-associated antigen 1 and intercellular adhesion molecule 2 interactions. (aacrjournals.org)
  • Lymphocyte function-associated antigen 1 (LFA-1) is found on leukocytes is involved in recruitment to the site of infection. (allergycases.org)
  • The lymphocyte function-associated antigen 1 (LFA-1) is a member of the beta2-integrin family and plays a pivotal role for T cell activation and leukocyte trafficking under inflammatory conditions. (mdpi.com)
  • Intercellular adhesion molecule-1 (ICAM1) is a ligand for lymphocyte function-associated (LFA) antigens. (ihcworld.com)
  • CD11a is a subunit of LFA-1, a cell surface molecule involved in T cell mediated immune responses. (springer.com)
  • CD11a - Subunit of LFA-1, a membrane glycoprotein that provides cell-cell adhesion by interaction with ICAM-1. (allergycases.org)
  • Integrin alpha M (ITGAM) is one protein subunit that forms the heterodimeric integrin alpha-M beta-2 (αMβ2) molecule, also known as macrophage-1 antigen (Mac-1) or complement receptor 3 (CR3) ITGAM is also known as CR3A, and cluster of differentiation molecule 11B (CD11B). (allergycases.org)
  • The results in this paper show that the mechanism of stimulation by porins of THP-1 cells enriched in CD14 receptor after treatment with 1,25-dihydroxyvitamin D 3 (vitamin D 3 ) is independent of this receptor, but is partially dependent on CD11a/18 integrins. (microbiologyresearch.org)
  • The β2 integrins are also known as the LFA-1 family or CD11a-cCD18. (allergycases.org)
  • CD11a plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. (fishersci.com)
  • Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of CD11a to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. (fishersci.com)
  • The model accounts for the continually changing number of CD11a molecules available for removing hu1124 from the circulation based on prior exposure of cells expressing CD11a to hu1124. (springer.com)
  • In addition, the model also accounts for saturation of CD11a molecules by hu1124 at drug concentrations of approximately 10 μg/ml, thereby reducing the clearance rate of hu1124 with increasing dose. (springer.com)
  • This altered migration pattern likely results from changes in the expression of leukocyte adhesion molecules such as L-selectin and CD11a. (physiology.org)
  • To identify the intracellular signals which increase the adhesiveness of leukocyte function-associated antigen 1 (LFA-1), we established an assay system for activation-dependent adhesion through LFA-1/intercellular adhesion molecule 1 ICAM-1 using mouse lymphoid cells reconstituted with human LFA-1 and then introduced constitutively active forms of signaling molecules. (asm.org)
  • Staphylococcal enterotoxins are classified as superantigens that act by linking T-cell receptor with MHC class II molecules, which are expressed on classical antigen-presenting cells (APC). (frontiersin.org)
  • Staphylococcal enterotoxins are superantigens due its properties to induce extensive proliferation of T cells mediated by cross-linking of the variable region of the β chain of the T-cell receptor (TCR) with MHC class II molecules on antigen-presenting cells (APC) such as macrophages and dendritic cells ( Fraser, 2011 ). (frontiersin.org)
  • To determine the safety and immunologic and clinical efficacy of a dendritic cell vaccine modified to hyperexpress costimulatory molecules and tumor antigen. (aacrjournals.org)
  • In this phase I study, we administered one or two cycles of four triweekly s.c./intradermal injections of ex vivo generated dendritic cells modified with a recombinant fowlpox vector encoding carcinoembryonic antigen (CEA) and a triad of costimulatory molecules [rF-CEA(6D)-TRICOM]. (aacrjournals.org)
  • To overcome these limitations, we have been studying strategies for genetic modification of dendritic cells with viral vectors encoding full length tumor antigens and costimulatory molecules. (aacrjournals.org)
  • K562, another human cell line of hematopoietic origin that has been extensively used in functional analyses of DC-SIGN and related molecules, lacks the principal molecules involved in the formation of immunological synaptic junctions, namely major histocompatibility complex (MHC) class II molecules and leukocyte function-associated antigen-1 (LFA-1). (pubmedcentralcanada.ca)
  • The intercellular adhesion molecules that form this junction have not been identified, although it has been reasoned that this structure may have similarities to the immunological synapse formed between antigen presenting cells (APCs) and their T cell conjugates. (pubmedcentralcanada.ca)
  • MHC class II molecules play a central role in immune responses through the presentation of processed antigens derived from endogenous and exogenous proteins that access endocytic pathways [ 14 - 17 ]. (pubmedcentralcanada.ca)
  • Antigens presented on MHC class II molecules are scanned by a T cell receptor complex that includes the CD4 molecule. (pubmedcentralcanada.ca)
  • Investigational products: LEISH-F3 (recombinant protein antigen) + GLA-SE (adjuvant) leishmaniasis vaccine and LEISH-F3 (recombinant protein antigen) + MPL-SE (adjuvant) leishmaniasis vaccine. (clinicaltrials.gov)
  • This study is a randomized, open-label clinical trial designed to evaluate the safety, tolerability, and immunogenicity of the LEISH-F3 recombinant protein antigen formulated with GLA-SE, MPL-SE, or SE adjuvant in healthy adults 18 to 49 years of age. (clinicaltrials.gov)
  • The combination of GLA-SE with a recombinant protein antigen (LEISH-F3) results in a Th1-type T cell response. (clinicaltrials.gov)
  • This is present on both T and B cells and binds to ICAM-1 (CD54) on antigen presenting cells. (brainscape.com)
  • The hypothesis is that PLP-BPI binds simultaneously to MHC-II and ICAM-1 on the antigen-presenting cell and inhibits the formation of the immunological synapse during T cell and antigen-presenting cell interactions. (aspetjournals.org)
  • It binds to ICAM-1 on antigen-presenting cells and functions as an adhesion molecule. (allergycases.org)
  • CL647-65194 targets CD11a in FC applications and shows reactivity with Human samples. (ptglab.com)
  • We identified Mono-7D6-Fab, which biophysically altered TCR/CD3 when bound and functionally enhanced immune reactivity to several weak antigens in vitro, including a gp100-derived peptide associated with melanoma. (sciencemag.org)
  • One of the receptor-mediated pharmacokinetic/pharmacodynamic models which was developed describes the dynamic interaction of hu1124 binding to CD11a, resulting in the removal of hu1124 from the circulation and reduction of cell surface CD11a. (springer.com)
  • Upon recognition of peptide-major histocompatibility complex (pMHC) antigens, T cell receptor (TCR)/CD3 triggering induces signal transduction that requires receptor clustering ( 1 , 2 ) but may be influenced by biophysical changes proposed to occur in TCR ( 3 - 8 ) and CD3 ( 9 - 12 ). (sciencemag.org)
  • Mouse monoclonal to CD11a.4A122 reacts with CD11a, a 180 kDa molecule. (a-443654.com)
  • Discovery and development of 5-[(5S,9R)-9- (4-cyanophenyl)-3-(3,5-dichlorophenyl)-1- methyl-2,4-dioxo-1,3,7-triazaspiro[4.4]non- 7-yl-methyl]-3-thiophenecarboxylic acid (BMS-587101)--a small molecule antagonist leukocyte function associated antigen-1. (rcsb.org)
  • Sarantos MR, Raychaudhuri S, Lum AF, Staunton DE, Simon SI: Leukocyte function-associated antigen 1-mediated adhesion stability is dynamically regulated through affinity and valency during bond formation with intercellular adhesion molecule-1. (exbio.cz)
  • 6 Both LFA-1 and intercellular adhesion molecule (ICAM)-1 are thought to play important roles in the pathogenesis of autoimmune disorders, and prior studies have shown that interference with adhesion molecule function, including CD11a, decreases histologic and clinical expression of endotoxin-induced uveitis. (arvojournals.org)
  • Proteolipid protein (PLP)-BPI was designed by conjugating two peptides, an encephalitogenic epitope of proteolipid protein (PLP 139-151 ) and an intercellular adhesion molecule-1-binding peptide derived from α L integrin (CD11a 237-246 ), via a spacer peptide. (aspetjournals.org)
  • Lipopolysaccharide (LPS) of Gram-negative bacteria and several surface components of Gram-positive bacteria utilize CD14 and CD11a/18 as cellular receptors to induce expression and release of cytokines. (microbiologyresearch.org)
  • Adaptive immunity is mediated by antigen receptors that can induce weak or strong immune responses depending on the nature of the antigen that is bound. (sciencemag.org)
  • CD11a is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. (fishersci.com)
  • CD11a is the a chain of the leukocyte function associated antigen-1 (LFA-1a), and is expressed on all leukocytes including T and B cells, monocytes, and granulocytes, but is absent on non-hematopoietic tissue and human platelets. (a-443654.com)
  • Flow cytometry analysis revealed a decline in L-selectin expression and an increase in CD11a expression and ICAM-1 on the surface of ConA-treated T cells. (physiology.org)
  • This process enables naive cells to increase the frequency of encounters with cognate antigens ( 9 , 26 ). (physiology.org)
  • Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-human CD11a (MEM-25) FITC. (exbio.cz)
  • In addition, we found a disease-induced depletion of T cells with high expression of the LFA-1 antigen, particularly in the CD4 + subset. (ajtmh.org)
  • A common goal of cancer vaccines in development is the activation of high levels of antigen-specific T cells. (aacrjournals.org)
  • Because of the crucial role of dendritic cells in adaptive immunity and their potent activity in animal tumor models, numerous pilot studies have evaluated immunotherapy with dendritic cells loaded with antigen in the form of peptide, protein, DNA, mRNA, tumor lysates, tumor fusions, and viral vectors ( 1 ). (aacrjournals.org)
  • We report that the intrinsically inert monovalent engagement of TCR/CD3 can specifically enhance physiologic T cell responses to weak antigens in vitro and in vivo without stimulating antigen-unengaged T cells and without interrupting T cell responses to strong antigens, an effect that we term as "co-potentiation. (sciencemag.org)
  • Anti-CD11a treatment improved visual function, reduced macular thickness, and increased the level of CD56 bright regulatory NK cells in patients with uveitic macular edema refractory to other immunosuppressive medications. (arvojournals.org)
  • VLA ("very late antigens") received their name because α1β1 and α2β1 were expressed on T cells 2 to 4 weeks after repetitive stimulation in vitro in the early experiments. (allergycases.org)
  • The interaction with antigen-presenting cells is also a dynamic one in which the T cell crawls across the cell surface in an LFA-1-dependent manner, scanning for antigenic peptides. (rupress.org)
  • 13 CD169 + macrophages directly present captured antigen to T cells or natural killer (NK) T cells 17 and are adept at transferring antigen to CD8α + DC and B cells. (bloodjournal.org)
  • This substudy will investigate whether, as in murine cells, cell surface markers (CD11a, CD49d) can be used as a surrogate to identify protective immune CD4+ T cells in human subjects receiving a vaccine antigen. (clinicaltrials.gov)
  • Specifically, Dr. Trauger will discuss confirmation of the specificity of Cytolin ® for selected blood cells, its unique binding site on its target antigen, CD11a, its ability to bind directly to HIV, and its lack of immunosuppression of HIV-specific cytotoxic T-cell ("CTL") activity. (fiercebiotech.com)
  • Here, we show that in response to viral or bacterial infection, antigen-specific CD8 T cells migrated to nonlymphoid tissues and were present as long-lived memory cells. (sciencemag.org)
  • After encounter with antigen, CD8 T cells differentiate into effector cells, which form a crucial arm of the adaptive immune response against intracellular pathogens through the action of cytokines and cell-mediated cytotoxicity ( 1 , 2 ). (sciencemag.org)
  • To address these questions, we compared antigen-specific effector and memory CD8 T cells in lymphoid and nonlymphoid tissues after viral or bacterial infections. (sciencemag.org)
  • MHC class I tetramers containing the appropriate antigenic peptide allow identification of antigen-specific CD8 T cells within an entire lymphocyte population ( 6 , 7 , 20 , 21 ). (sciencemag.org)
  • CD11a is expressed on the plasma membrane of leukocytes in a low-affinity conformation. (fishersci.com)
  • CD11a is expressed by all leukocytes. (thermofisher.com)
  • The objective of this study is to evaluate the activity of a novel peptide, i.e., bifunctional peptide inhibitor (BPI), which targets the immunological synapse and inhibits autoimmune responses in an antigen-specific manner. (aspetjournals.org)
  • When hu1124 levels fell below 3 μg/ml, the drug was rapidly cleared from the circulation and expression of CD11a returned to normal within 7-10 days thereafter. (springer.com)
  • Early indices of both peripheral CD4 and CD8 T cell activation, including IFN-γ production and increases in CD44 and CD11a expression, are attenuated in mast cell-deficient (W/W v ) mice after myelin oligodendrocyte glycoprotein 35-55 priming when compared to WT animals. (wiley.com)
  • It also appears to be crucial in the maintenance of regulatory T cell- (Treg-) mediated immunotolerance toward self-antigens, supported by the fact that IL-2-deficient mice develop severe autoimmunity with very low Treg and very high effector T numbers [7-9]. (thefreelibrary.com)
  • Release of cardiac self-antigens can subsequently lead to breakdown of heart-specific tolerance tissue and can evolve into autoimmune-mediated inflammation sustaining the disease upon eradication of the virus. (mdpi.com)
  • Among the many tumor antigens described, the model antigen, carcinoembryonic antigen (CEA), is of particular interest because it is widely expressed in gastrointestinal, lung, breast, and other malignancies. (aacrjournals.org)
  • CD11a functions in the immune system. (wikipedia.org)
  • Immunologic tolerance is a state of immune unresponsiveness specific to a particular antigen or set of antigens induced by previous exposure to that antigen or set. (medscape.com)
  • Immune response to the hepatitis B antigen in the RTS,S/AS01 malaria vaccine, and co-administration with pneumococcal conjugate and rotavirus vaccines in African children: a randomized controlled trial. (bioportfolio.com)
  • This pathway modulates the immune response to circulating particulate antigens. (bloodjournal.org)
  • This is the first report of a role for CD169 in the capture of exosomes and its potential to mediate the immune response to exosomal antigen. (bloodjournal.org)
  • Exosomes are a potential source of self-antigen for modulating the immune response against self-tissues, including tumors. (bloodjournal.org)
  • 13 , 17 CD169 + macrophages sample a wide variety of antigens and participate in generation of immunity to tumors and viruses but may also down-regulate immune responses to self-tissue. (bloodjournal.org)
  • PLP-BPI is constructed via conjugation of myelin proteolipid protein (PLP 139-151 ) with CD11a 237-246 -derived peptide (LABL) via a spacer. (aspetjournals.org)
  • Mice that received i.v. injections of PLP-BPI showed significantly lower EAE disease scores and incidence than those treated with vehicle, PLP 139-151 peptide only, CD11a 237-246 peptide only, unlinked mixture of PLP 139-151 , and CD11a 237-246 peptides, or other control peptides. (aspetjournals.org)
  • Both have been engineered to express tumor antigen, such as CEA, and have been tested in murine ( 10 ) and human ( 11 , 12 ) studies. (aacrjournals.org)
  • Cross-linking of the TCR transiently converts LFA-1 to a high avidity state and thus provides a mechanism for regulating cellular adhesion and de-adhesion in an antigen-specific manner. (sciencemag.org)
  • A human lymphocyte-associated antigen involved in cell-mediated lympholysis. (springer.com)
  • The HB-6, CDw75, and CD76 Differentiation Antigens are Unique Cell-Surface Carbohydrate Determinants Generated by the .beta. (freepatentsonline.com)
  • White Cell Differentiation Antigens" McMichael, A. J., Oxford University Press. (freepatentsonline.com)
  • B-cell and plasma antigens: new and previously defined clusters" In Leukocyte Typing III. (freepatentsonline.com)
  • Role of the CD22 Human B Cell Antigen in B Cell Triggering by Anti-Immunoglobulin," J. Immunol. (freepatentsonline.com)
  • B5.2 The carbohydrate moiety of the CD22 antigen can be modulated by inhibitors of the glycosylation pathway," B-cell antigens-papers, 65-67. (freepatentsonline.com)
  • In some cases, antigen targets exist in different forms, notably in the circulation and expressed at the cell surface. (springer.com)
  • It is also well known that LFA-1/ICAM-1-mediated adhesion establishes and strengthens the T-cell-antigen-presenting cell (APC) contact, which is a critical event for T-cell activation ( 14 , 51 , 69 ). (asm.org)
  • In vivo, Mono-7D6-Fab induced T cell antigen-dependent therapeutic responses against melanoma lung metastases, an effect that synergized with other anti-melanoma immunotherapies to significantly improve outcome and survival. (sciencemag.org)
  • Consistent with these findings, the presence of hematopoietic-associated markers (CD11a, CD14, CD45, CD86, and histocompatible locus antigen-DR [HLA-DR]) detected on the heterogeneous SVF cell population decreased upon subsequent passage of the ASCs. (nih.gov)
  • Diseases associated with CD11a dysfunction include benign cephalic histiocytosis and leukocyte adhesion deficiency. (fishersci.com)
  • If some reported covariates, such as the circulating antigen level or tumour size, are likely to be correlated to antigen mass, others, such as disease activity or disease type, may contain little information on the amount of antigenic targets. (springer.com)
  • Antigens, Western, ELISA and other in vitro binding or in vivo functional assays, and protein-protein interaction studies. (creativebiomart.net)