Antibody-Dependent Enhancement: Enhancement of viral infectivity caused by non-neutralizing antibodies. There are at least two mechanisms known to account for this: mediation by Fc receptors (RECEPTORS, FC) or by complement receptors (RECEPTORS, COMPLEMENT). Either the virus is complexed with antiviral IMMUNOGLOBULIN G and binds to Fc receptors, or virus is coated with antiviral IMMUNOGLOBULIN M and binds to complement receptors.Antibody-Dependent Cell Cytotoxicity: The phenomenon of antibody-mediated target cell destruction by non-sensitized effector cells. The identity of the target cell varies, but it must possess surface IMMUNOGLOBULIN G whose Fc portion is intact. The effector cell is a "killer" cell possessing Fc receptors. It may be a lymphocyte lacking conventional B- or T-cell markers, or a monocyte, macrophage, or polynuclear leukocyte, depending on the identity of the target cell. The reaction is complement-independent.Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Immunoglobulin G: The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.Killer Cells, Natural: Bone marrow-derived lymphocytes that possess cytotoxic properties, classically directed against transformed and virus-infected cells. Unlike T CELLS; and B CELLS; NK CELLS are not antigen specific. The cytotoxicity of natural killer cells is determined by the collective signaling of an array of inhibitory and stimulatory CELL SURFACE RECEPTORS. A subset of T-LYMPHOCYTES referred to as NATURAL KILLER T CELLS shares some of the properties of this cell type.Cytotoxicity, Immunologic: The phenomenon of target cell destruction by immunologically active effector cells. It may be brought about directly by sensitized T-lymphocytes or by lymphoid or myeloid "killer" cells, or it may be mediated by cytotoxic antibody, cytotoxic factor released by lymphoid cells, or complement.Antibody Specificity: The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.

In vitro antibody-dependent enhancement assays are insensitive indicators of in vivo vaccine enhancement of equine infectious anemia virus. (1/83)

We have previously demonstrated a high propensity for enhancement of virus replication and disease resulting from experimental immunization of ponies with a baculovirus recombinant envelope (rgp90) vaccine from equine infectious anemia virus (EIAV). The current studies were undertaken to examine the correlation between the observed in vivo vaccine enhancement and in vitro assays for antibody-dependent enhancement (ADE) of EIAV replication. Toward this goal an optimized EIAV in vitro enhancement assay was developed using primary equine macrophage cells and used to evaluate the enhancement properties of immune serum taken from rgp90 immunized ponies that displayed various levels of vaccine enhancement after experimental challenge with EIAV. For comparison, we analyzed in parallel immune serum samples from a group of ponies immunized with a viral envelope subunit vaccine (LL-gp) that produced sterile protection from EIAV challenge. The results of these assays demonstrated that the rgp90 immune serum had a greater propensity for in vitro enhancement of EIAV replication than serum from the protected LL-gp immunized ponies; in vitro enhancement levels for the rgp90 immune sera averaged about 1.5, with a maximum enhancement value of about 2.0. While distinguishing between immune serum produced by the rgp90 and LL-gp immunizations, the in vitro enhancement assay failed to reliably correlate with the severity of in vivo enhancement observed among the rgp90 vaccine recipients. Vaccinated ponies that experienced moderate to no disease signs displayed levels of in vitro enhancement similar to those of ponies that experienced severe and fatal enhancement of disease after viral challenge. The observed in vitro enhancement was demonstrated to be dependent on serum immunoglobulin, but independent of complement. These studies demonstrate in the EIAV system that in vitro ADE assays appear to be relatively insensitive indicators of the severity of in vivo enhancement and that relatively low levels of in vitro ADE can be associated with severe to fatal enhancement of virus replication and disease in vivo. These observations suggest that relatively low levels of serum ADE observed in other lentivirus systems, including HIV-1, may have more profound effects on in vivo virus replication and disease than previously recognized.  (+info)

Infection of human cells by dengue virus is modulated by different cell types and viral strains. (2/83)

Although prior studies have investigated cellular infection by dengue virus (DV), many have used highly passaged strains. We have reassessed cellular infection by DV type 2 (DV2) using prototype and low-passage isolates representing genotypes from different geographic areas. We observed marked variation in the susceptibility to infection among cell types by different DV2 strains. HepG2 hepatoma cells were susceptible to infection by all DV2 strains assayed. Although the prototype strain generated higher titers of secreted virus than the low-passage isolates, this difference did not correspond to positive- or negative-strand viral RNA levels and thus may reflect variation in efficiency among DV2 isolates to translate viral proteins or package and/or secrete virus. In contrast, human foreskin fibroblasts were susceptible to the prototype and low-passage Thai isolates but not to five Nicaraguan strains tested, as reflected by the absence of accumulation of negative-strand viral RNA, viral antigen, and infectious virus. A similar pattern was observed with the antibody-dependent pathway of infection. U937 and THP-1 myeloid cells and peripheral blood monocytes were infected in the presence of enhancing antibodies by the prototype strain but not by low-passage Nicaraguan isolates. Again, the barrier appeared to be prior to negative-strand accumulation. Thus, depending on the cell type and viral isolate, blocks that limit the production of infectious virus in vitro may occur at distinct steps in the pathway of cellular infection.  (+info)

Specific ablation of antiviral gene expression in macrophages by antibody-dependent enhancement of Ross River virus infection. (3/83)

Ross River virus (RRV) is an indigenous Australian arthropod-borne alphavirus responsible for epidemic polyarthritis (EPA), myalgia, and lethargy in humans. Macrophages and monocytes have been associated with human RRV disease, and previous studies have shown that RRV is capable of infecting macrophages via both a natural virus receptor and by Fc receptor-mediated antibody-dependent enhancement (ADE). Similar to other viruses, such as human immunodeficiency virus and dengue virus, ADE infection results in dramatic RRV growth increases for in vitro macrophage cultures. This study demonstrates that RRV could resist lipopolysaccharide (LPS)-induced antiviral activity in macrophage cultures when infection was via the ADE pathway. Investigation of this infection pathway found that RRV was able to suppress the transcription and translation of key antiviral genes (tumor necrosis factor and inducible nitric oxide synthase) in LPS-stimulated macrophages by disrupting the transcription into mRNA of the genes coding for the associated transcription factors IRF-1 and NF-kappaB. The transcription of non-antiviral control genes was not perturbed by RRV-ADE infection, and de novo protein synthesis also was not significantly affected in RRV-ADE infected cells. The ADE pathway of infection allowed RRV to specifically target antiviral genes in macrophages, resulting in unrestricted virus replication. As ADE has been observed for several virus families and associated with disease and adverse vaccination outcomes, these findings may have broad relevance to viral disease formation and antiviral vaccination strategies.  (+info)

Infectivity-enhancing antibodies to Ebola virus glycoprotein. (4/83)

Ebola virus causes severe hemorrhagic fever in primates, resulting in mortality rates of up to 100%, yet there are no satisfactory biologic explanations for this extreme virulence. Here we show that antisera produced by DNA immunization with a plasmid encoding the surface glycoprotein (GP) of the Zaire strain of Ebola virus enhances the infectivity of vesicular stomatitis virus pseudotyped with the GP. Substantially weaker enhancement was observed with antiserum to the GP of the Reston strain, which is much less pathogenic in humans than the Ebola Zaire and Sudan viruses. The enhancing activity was abolished by heat but was increased in the presence of complement system inhibitors, suggesting that heat-labile factors other than the complement system are required for this effect. We also generated an anti-Zaire GP monoclonal antibody that enhanced viral infectivity and another that neutralized it, indicating the presence of distinct epitopes for these properties. Our findings suggest that antibody-dependent enhancement of infectivity may account for the extreme virulence of the virus. They also raise issues about the development of Ebola virus vaccines and the use of passive prophylaxis or therapy with Ebola virus GP antibodies.  (+info)

Enhancement of adenovirus vector entry into CD70-positive B-cell Lines by using a bispecific CD70-adenovirus fiber antibody. (5/83)

Although many recombinant adenovirus vectors (rAd) have been developed, especially by using group C adenoviruses, to transfer and express genes, such rAd do not readily infect B-cell lines due to the lack of the coxsackievirus-adenovirus receptor. Bispecific antibodies have been used in different cell systems to facilitate entry of rAd into otherwise nonpermissive cells. Bispecific antibody is synthesized by covalently linking two monoclonal antibodies with distinct specificities. It has been shown that lymphoproliferative tumors commonly express the cell surface protein CD70, while this receptor is normally expressed on only a small subset of highly activated B cells and T cells. We therefore investigated whether a bispecific antibody with specificities for the adenovirus fiber protein and CD70 can facilitate rAd entry and subsequent expression of rAd-encoded genes in CD70-positive B cells. We found high CD70 expression on Epstein-Barr virus (EBV)-transformed lymphoblastoid cell lines (LCLs), as well as some, but not all, Burkitt lymphoma (BL) lines. We show here that rAd encoding green fluorescent protein (Ad-GFP) infects EBV-transformed LCLs and a CD70-positive BL line 10- to 20-fold more efficiently in the presence of the CD70-fiber bispecific antibody. In contrast, the bispecific antibody does not enhance Ad-GFP infection in CD70-deficient BL cells. Using the CD70-fiber bispecific antibody, we increased the ability of rAd vectors encoding the EBV immediate-early proteins BZLF1 and BRLF1 to induce the lytic form of EBV infection in LCLs. These results indicate that the CD70-fiber bispecific antibody can enhance rAd infection of CD70-positive B cells and suggest the use of this vector to explore EBV-positive LCLs.  (+info)

Antibody-dependent enhancement of coxsackievirus B4 infectivity of human peripheral blood mononuclear cells results in increased interferon-alpha synthesis. (6/83)

IgG devoid of neutralizing activity and isolated from donor plasma by chromatography formed immune complexes with coxsackievirus B4 (CVB4) and significantly increased the infection of peripheral blood mononuclear cells with CVB4. The major host cells for CVB4 infection enhanced with IgG are monocytic CD14+ cells. The roles of CVB and adenovirus receptor and Fcgamma receptor II and III have been shown. Increased viral replication and the release of infectious particles were demonstrated when interferon (IFN)-alpha produced by infected cells was first neutralized by use of antibodies. The CVB4 IgG-induced synthesis of IFN-alpha by monocytes reflected entry and uncoating of CVB4 but not of viral replication and required the presence of CVB4 RNA inside the cells. Thus, CVB4 can infect monocytes by an antibody-dependent mechanism through interactions between the virus, antiviral antibodies, and specific receptors that result in IFN-alpha production.  (+info)

Enhancement of coxsackievirus B3 infection by antibody to a different coxsackievirus strain. (7/83)

Group B coxsackieviruses (CVBs) are a major cause of viral myocarditis and pancreatitis in humans and produce a similar pattern of disease in inbred strains of mice. As there are six strains of CVBs, individuals can be infected with multiple serotypes. This raises the possibility of antibody enhancement of infectivity (AEI) by cross-reactive but non-neutralizing antibody to a different strain from a prior infection. To determine whether AEI plays a role in coxsackievirus pathogenesis, an in vitro system using the murine macrophage cell line J774.1 was tested for enhanced infection when incubated with CVB3 plus anti-CVB2 antibody. Yields of virus were found to increase by 10-50-fold and the percentage of infected cells increased proportionately. The effect was Fc-mediated as F(ab')2 fragments of the antibody could not mediate the effect. To determine whether AEI could also be demonstrated in vivo CVB3 was injected into 5-week-old mice together with mouse polyclonal anti-CVB2. Controls included mice injected with PBS or CVB3 alone. Results showed that the titres of virus in tissues of animals injected with virus plus antibody were 1-2 logs higher than when virus was injected alone. This was accompanied by greater histopathological damage, particularly in the heart. These results have implications for human disease as infection with multiple strains likely occurs during the lifetime of an individual.  (+info)

Complement-mediated enhancement of antibody function for neutralization of pseudotype virus containing hepatitis C virus E2 chimeric glycoprotein. (8/83)

We previously reported a number of features of hepatitis C virus (HCV) chimeric glycoproteins related to pseudotype virus entry into mammalian cells. In this study, pseudotype virus was neutralized by HCV E2 glycoprotein-specific antibodies and infected human sera. Neutralization (50% reduction of pseudotype virus plaque formation) was observed with two human immunoglobulin G1 monoclonal antibodies (MAbs) at concentrations of between 2.5 and 10 microg/ml. A hyperimmune rabbit antiserum to an E2 hypervariable region 1 (HVR1) mimotope also exhibited an HCV E2 pseudotype virus neutralization titer of approximately 1/50. An E1 pseudotype virus used as a negative control was not neutralized to a significant level (<1/10) by these MAbs or rabbit antiserum to E2 HVR1. Since HCV probably has a lipid envelope, the role of complement in antibody-mediated virus neutralization was examined. Significant increases in the neutralization titers of the human MAbs (approximately 60- to 160-fold higher) and rabbit antiserum to HVR1 mimotopes (approximately 10-fold higher) were observed upon addition of guinea pig complement. Further, these studies suggested that complement activation occurred primarily by the classical pathway, since a deficiency in the C4 component led to a significant decrease in the level of virus neutralization. This same decrease was not observed with factor B-deficient complement. We also determined that 9 of 56 HCV-infected patient sera (16%) had detectable pseudotype virus neutralization activity at serum dilutions of between 1/20 and 1/50 and that complement addition enhanced the neutralization activity of some of the HCV-infected human sera. Taken together, these results suggest that during infection, HCV E2 glycoprotein induces a weak neutralizing antibody response, that those antibodies can be measured in vitro by the surrogate pseudotype virus plaque reduction assay, and that neutralization function can be augmented by complement.  (+info)

*Antibody-dependent enhancement

Gras, GS; Dormont, D (1991). "Antibody-dependent and antibody-independent complement-mediated enhancement of human ... or antibody-dependent cellular cytotoxicity-mediating antibodies (ADCC) in the serum contains infection enhancing antibodies( ... Antibody-dependent enhancement (ADE) occurs when non-neutralising antiviral proteins facilitate virus entry into host cells, ... Takada, A; Feldmann, H; Ksiazek, TG; Kawaoka, Y (2003). "Antibody-Dependent Enhancement of Ebola Virus Infection". Journal of ...

*Fc receptor

... by a mechanism known as antibody-dependent enhancement of infection. There are several different types of Fc receptors ( ... or infected cells by antibody-mediated phagocytosis or antibody-dependent cell-mediated cytotoxicity. Some viruses such as ... This process is known as antibody-dependent cell-mediated cytotoxicity (ADCC). FcγRIII on NK cells can also associate with ... Another process involving Fc receptors is called antibody-dependent cell-mediated cytotoxicity (ADCC). During ADCC, FcγRIII ...

*Ram Samudrala

Viral entry inhibitors block dengue antibody-dependent enhancement in vitro. Antiviral Research 89: 71-74, 2011. Computational ... McDermott J, Guerquin M, Frazier Z, Chang AN, Samudrala R. BIOVERSE: Enhancements to the framework for structural, functional, ... Samudrala R, Moult J. An all-atom distance-dependent conditional probability discriminatory function for protein structure ...

*Dengue fever

One of the concerns is that a vaccine could increase the risk of severe disease through antibody-dependent enhancement (ADE). ... The most widely accepted hypothesis is that of antibody-dependent enhancement (ADE). The exact mechanism behind ADE is unclear ... "Sanofi restricts dengue vaccine but downplays antibody enhancement". CIDRAP. Retrieved 2 December 2017. Webster DP, Farrar J, ... Various antibodies are generated; some bind closely to the viral proteins and target them for phagocytosis (ingestion by ...

*Sunetra Gupta

Ferguson, N.; Anderson, R.; Gupta, S. (1999). "The effect of antibody-dependent enhancement on the transmission dynamics and ...

*Eva Harris

In particular, the critical problem of the dengue virus infection, called "antibody-dependent enhancement" (ADE), is reproduced ... the antibodies produced by the immune system can lead to increased severity of the second dengue infection, instead of ... focusing on functional characterization of antibodies and B cell memory response, host gene expression profiling, and viral ...

*Dengue virus

Among the possible causes are cross-serotypic immune response, through a mechanism known as antibody-dependent enhancement, ... when infected with another serotype due to antibody-dependent enhancement. When infected with dengue virus, the immune system ... Unfortunately D7 proteins provoke immune responses, which raise anti-D7 antibody levels. These antibodies inhibit the function ... The human antibodies Ede1-C10, Ede2-A11 and Ede2-B7 potently neutralize all four DENV serotypes. Their Fab or scFv fragments ...

*Zika virus vaccine

... the vaccine needs to minimize the possibility of antibody-dependent enhancement of dengue virus infection. As of March 31, 2017 ... The goal of a Zika virus vaccine is to elicit protective antibodies against the Zika virus to prevent infection and severe ...

*Dengue fever outbreaks

One model to explain this process is known as antibody-dependent enhancement (ADE), which allows for increased uptake and ...

*Ade

... a nucleobase Adobe Digital Editions Adverse Drug Event Antibody-dependent enhancement Application Development Environment ...

*Influenza A virus subtype H7N9

This immune phenomenon is called antibody-dependent enhancement (ADE), and is perhaps best known in cases of Dengue fever when ... It is thought to occur when weakly cross-reactive antibodies form bridging complexes to facilitate uptake and replication of ... The phenomenon of cross-reacting antibodies that facilitate infection is best known in dengue infections, according to ... contacts of the infected patients did not become ill and they all tested negative for haemagglutination inhibition antibodies ...

*Dengue vaccine

... people who have not been previously infected with the dengue virus because of the phenonmenon of antibody-dependent enhancement ... "Sanofi restricts dengue vaccine but downplays antibody enhancement". CIDRAP. Retrieved 2 December 2017. "DOJ orders NBI to ...

*Original antigenic sin

Antibody-dependent enhancement Cell mediated immunity Humoral immunity Polyclonal response FDA Center for Biologics Evaluation ... these antibodies inhibit the activation of higher-affinity naive B cells that would be able to make more effective antibodies ... However, the antibodies produced by these B cells generally ineffectively bind to the altered epitopes. In addition, ... Researchers found reduced antibody responses to the 2009 pandemic H1N1 influenza vaccine in individuals who had been vaccinated ...

*List of MeSH codes (G04)

... antibody-dependent enhancement MeSH G04.185.515.880.210 --- cell transformation, viral MeSH G04.185.515.880.225 --- ... antibody-dependent cell cytotoxicity MeSH G04.610.270.500 --- macrophage activation MeSH G04.610.484.100 --- clonal anergy MeSH ... antibody affinity MeSH G04.610.143.140 --- antigenic modulation MeSH G04.610.143.232 --- binding sites, antibody MeSH G04.610. ... antibody diversity MeSH G04.610.626.073 --- antigenic variation MeSH G04.610.626.320 --- gene rearrangement, b-lymphocyte MeSH ...

*Index of immunology articles

... antibodies Antibody Antibody opsonization Antibody-dependent cell-mediated cytotoxicity Antibody-dependent enhancement Antigen ... Molecular mimicry Monoclonal antibody Monoclonal antibody therapy Monokine Mononuclear phagocyte system Monospecific antibody ... Beta-2 microglobulin Binding antibody Biological response modifiers Blocking antibody Blotto (biology) BNAber Body odor Bone ... antibodies Polyclonal B cell response Polymersome Pox party Precipitin Premunition Premunity Primary and secondary antibodies ...

*Cancer immunotherapy

... enhancement of antibody-dependent cell-mediated cytotoxicity; and CR3-dependent cellular cytotoxicity. Complement-dependent ... Human antibodies have completely human DNA. Antibody-dependent cell-mediated cytotoxicity (ADCC) requires antibodies to bind to ... Naked monoclonal antibodies are antibodies without added elements. Most antibody therapies use this antibody type. Conjugated ... Once bound to a cancer antigen, antibodies can induce antibody-dependent cell-mediated cytotoxicity, activate the complement ...

*Hormonal breast enhancement

A neutralizing antibody for HGF, but not for IGF-1 or EGF, was found to attenuate the proliferation of breast epithelial tissue ... dependent on dose and type of estrogen administered), as well as increases levels of insulin-like growth factor-binding protein ... Hormonal breast enhancement or augmentation is a highly experimental potential medical treatment for the breasts in which ... Moreover, a trial of hormonal breast enhancement in 45 young women with very high doses (80 mg/injection) of intramuscular, ...

*HLA-DQB2

1994). "HIV-1 gp41 binding proteins and antibodies to gp41 could inhibit enhancement of human Raji cell MHC class I and II ... 1994). "Envelope glycoproteins of HIV-1 interfere with T-cell-dependent B cell differentiation: role of CD4-MHC class II ...

*HLA-DQA2

1994). "HIV-1 gp41 binding proteins and antibodies to gp41 could inhibit enhancement of human Raji cell MHC class I and II ... 1994). "Envelope glycoproteins of HIV-1 interfere with T-cell-dependent B cell differentiation: role of CD4-MHC class II ... 1988). "Sequence analysis of HLA class II genes from insulin-dependent diabetic individuals". Hum. Immunol. 21 (4): 249-63. doi ...

*Tuftsin

The enhancement of antitumor immune response by immunomodulators is capable of stimulating reticuloendothelial and T-cell- ... Maximal effect was measured at tuftsin concentration 5x10-8 M. This process is highly specific and dependent on the structural ... Conjugates with polytuftsin retain tuftsin-like effects and increase the epitope specific antibody production. Tuftsin sequence ... The number of antigen-forming cells was increased by the injections of tuftsin T-dependent antigen. Tuftsin enhanced the ...

*Geoffrey W. Hoffmann

... a co-study on antibodies made in a normal immune response that bind both to foreign invaders and to antibodies with the same ... 5, 638-647 G. W. Hoffmann (1978) Incorporation of a Non-specific T Cell Dependent Helper Factor into a Network Theory of the ... and Reverse Enhancement. J. Immunol. 137, 61-68 R. B. Forsyth, G. W. Hoffmann (1990) A study of auto antiidiotypes to BSA, J. ... consisting of antibodies and lymphocytes that recognize not only things that are foreign to the body, but also each other. ...

*Development of analogs of thalidomide

This enhances natural and antibody dependent cellular cytotoxicity. Lenalidomide and pomalidomide are about 100-1000 times more ... increasing sensitivity to FAS mediated cell death and enhancement of TNF-related apoptosis inducing ligand. They have also been ... Lenalidomide is also approved for transfusion-dependent anemia due to low or intermediate-1-risk myelodysplastic syndromes ... shown to cause dose dependent G0/G1 cell cycle arrest in leukemia cell lines where the analogs showed 100 times more potency ...

*H2AFY

Deng L, Wang D, de la Fuente C, Wang L, Li H, Lee CG, Donnelly R, Wade JD, Lambert P, Kashanchi F (Oct 2001). "Enhancement of ... Chadwick BP, Willard HF (Jun 2002). "Cell cycle-dependent localization of macroH2A in chromatin of the inactive X chromosome". ... "Novel tumor antigens identified by autologous antibody screening of childhood medulloblastoma cDNA libraries". International ...

*Outline of immunology

Antibody-dependent cell-mediated cytotoxicity (ADCC) Foreign Pernicious anemia Hemolytic disease of the newborn Autoimmune ... Prevention Immunostimulants Immunotherapy Activation immunotherapy Cancer immunotherapy Autologous immune enhancement therapy ... Antibodies Kinds of Antibodies Monoclonal antibodies Polyclonal antibodies Autoantibody Microantibody Neutralizing antibody ... Antibodies Immunoglobulin A (IgA) IgA1 IgA2 Immunoglobulin D (IgD) Immunoglobulin E (IgE) Immunoglobulin G (IgG) IgG1 IgG2 IgG3 ...

*Lectin

"Lectin-Dependent Enhancement of Ebola Virus Infection via Soluble and Transmembrane C-type Lectin Receptors". PLoS ONE. 8 (4 ... Lectins are similar to antibodies in their ability to agglutinate red blood cells.[medical citation needed] The toxicity of ...

*Lyme disease

The OspC antibodies kill any of the bacteria that have not been killed by the OspA antibodies. Canine Recombinant Lyme, ... The specific approach to their use is dependent on the individual affected and the stage of the disease. For most people with ... burgdorferi in ticks and for enhancement of spirochetemia in mice". Cell. 89 (7): 1111-9. doi:10.1016/S0092-8674(00)80298-6. ... IgM and IgG antibody levels may be elevated for years even after successful treatment with antibiotics. As antibody levels are ...
Antibody-dependent enhancement (ADE) occurs when non-neutralising antiviral proteins facilitate virus entry into host cells, leading to increased infectivity in the cells. Some cells do not have the usual receptors on their surfaces that viruses use to gain entry. The antiviral proteins (i.e., the antibodies) bind to antibody Fc receptors that some of these cells have in the plasma membrane. The viruses bind to the antigen binding site at the other end of the antibody. ADE is common in cells cultured in the laboratory, but rarely occurs in vivo except for dengue virus. This virus can use this mechanism to infect human macrophages, causing a normally mild viral infection to become life-threatening. The most widely known example of ADE occurs in the setting of infection with the dengue virus (DENV). DENV is a single-stranded positive-polarity RNA virus of the Flaviviridae family. It causes a disease of varying severity in humans, from dengue fever (DF), which is usually self-limited, to dengue ...
Read "Determination of antibody concentration as the main parameter in a dengue virus antibody-dependent enhancement assay using FcγR-expressing BHK cells, Archives of Virology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
DENV infection elicits primarily a poor quality immune response directing a high proportion of antibody against non-protective, potentially pathogenic epitopes and only a small proportion against potently neutralizing and protective epitopes. In this report we have shown the manipulation of these potentially pathogenic epitopes as a vaccine strategy [41] that can reduce ADE in vitro and in vivo. Immunization of mice demonstrated that knocking out immunodominant cross-reactive epitopes in the EDIIFP and EDIII did not significantly effect DENV-2 neutralization, however the removal of these epitopes dramatically altered the vaccine induced antibody repertoire and sera from vaccinated mice shows reduced ADE in vitro and reduced lethal enhancement of DENV in vivo. Such a strategy could be applicable to other DENV vaccine formats, however, it might not be applicable to DENV live-attenuated vaccines because mutations in the EDIIFP can be lethal [42].. Our findings demonstrate that by introducing ...
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Immunopathogenesis of Intense Dengue in Secondary Infections.The kinetics of viremia inside of a individual with secondary dengue, the timing of typical difficulties, and achievable mechanistic results in are proven. Early in secondary an infection (or Most important infection of infants), antibody-dependent enhancement is assumed to boost in vivo concentrations of virus.17 Antibody-dependent improvement is associated with the existence of non-neutralizing or subneutralizing levels of dengue virus-reactive IgG induced by a Principal an infection, or obtained passively in newborns. A large infected cell mass leads to elevated concentrations of acute-period reaction proteins, cytokines, and chemokines; technology of immune complexes; and use of enhance and launch of break up products. The activation, proliferation, and secretion of cytokines in tissues by memory T lymphocytes recognizing conserved and altered peptide ligands are postulated to incorporate on the inflammatory milieu throughout ...
Bacteriophage P2 is known for its exceptionally low rate of spontaneous (non-integrative) recombination, which however may be stimulated by ultraviolet irradiation of the phage. We show here that ligated dimers, made in vitro from mixtures of DNAs of
Antibody-dependent enhancement (ADE) has been proposed as a mechanism to explain dengue hemorrhagic fever (DHF) in the course of a secondary dengue infection. roles in virus attachment to cells and fusion with membranes, and is the major target for neutralizing antibody. It contains the main epitopes recognized by neutralizing antibodies (virus-particular and cross-reactive epitopes) [5,6]. This proteins offers three structural and practical domains: domain II provides the inner fusion peptide (in charge of the fusion of flaviviruses with their target cellular material) and domain III the cellular receptor-binding motifs [7,8]. Domains I and III consist of predominantly subcomplex- and type-particular epitopes, whereas domain II provides the main flavivirus group and subgroup cross-reactive epitopes [9C11]. M protein could be within two forms. In cell-connected (immature) virions, prM (the precursor of M proteins) is noticed, which forms a heterodimer with the Electronic protein (prM-Electronic ...
An Fc receptor is a protein found on the surface of certain cells - including, among others, B lymphocytes, follicular dendritic cells, natural killer cells, macrophages, neutrophils, eosinophils, basophils, human platelets, and mast cells - that contribute to the protective functions of the immune system. Its name is derived from its binding specificity for a part of an antibody known as the Fc (Fragment, crystallizable) region. Fc receptors bind to antibodies that are attached to infected cells or invading pathogens. Their activity stimulates phagocytic or cytotoxic cells to destroy microbes, or infected cells by antibody-mediated phagocytosis or antibody-dependent cell-mediated cytotoxicity. Some viruses such as flaviviruses use Fc receptors to help them infect cells, by a mechanism known as antibody-dependent enhancement of infection. There are several different types of Fc receptors (abbreviated FcR), which are classified based on the type of antibody that they recognize. The Latin letter ...
Antibodies that enhance human immunodeficiency virus (HIV) infectivity have been found in the blood of infected individuals and in infected or immunized animals. These findings raise serious concern for the development of a safe vaccine against acquired immunodeficiency syndrome. To address the in vivo relevance and mechanism of this phenomenon, antibody-dependent enhancement of HIV infectivity in peripheral blood macrophages, lymphocytes, and human fibroblastoid cells was studied. Neither Leu3a, a monoclonal antibody directed against the CD4 receptor, nor soluble recombinant CD4 even at high concentrations prevented this enhancement. The addition of monoclonal antibody to the Fc receptor III (anti-FcRIII), but not of antibodies that react with FcRI or FcRII, inhibited HIV type 1 and HIV type 2 enhancement in peripheral blood macrophages. Although enhancement of HIV infection in CD4+ lymphocytes could not be blocked by anti-FcRIII, it was inhibited by the addition of human immunoglobulin G ...
Live-attenuated vaccines (LAV) are reputed to be the most cost-effective tools for controlling epidemics. With increasing disease outbreaks caused by virus infections, vaccines will have to be delivered to both adults and children, who may have pre-existing cross-reactive antibodies due to previous exposure with an antigenically related virus strain. We and others have shown in vitro that cross-reactive antibodies can improve vaccine efficacy by enhancing LAV infection in Fc gamma-receptor (FcgR) expressing antigen-presenting cells (APCs), a process known as antibody-dependent enhancement (ADE). However, the relevance and occurrence of ADE has yet to be demonstrated clinically. We conducted an open-label trial where subjects are sequentially immunized with the inactivated Japanese Encephalitis (JE) vaccine (Ixiaro®) followed by a live-attenuated yellow fever (YF) vaccine (Stamaril®). To generate a range of cross-reactive antibodies concentrations, subjects were divided into 3 groups, where ...
Antibodies, Antibody-dependent Enhancement, Concentrations, Dengue, Dengue Virus, Disease, Epitopes, Fc Receptor, Glycoprotein, Histories, Human, Infection, Inhibition, Liposome, Monoclonal Antibodies, Mosquito, Mutations, Patients, Proteins, Therapeutic
Therapies to prevent maternal Zika virus (ZIKV) infection and its subsequent fetal developmental complications are urgently required. We isolated three potent ZIKV-neutralizing monoclonal antibodies (nmAbs) from the plasmablasts of a ZIKV-infected patient-SMZAb1, SMZAb2, and SMZAb5-directed against two different domains of the virus. We engineered these nmAbs with Fc LALA mutations that abrogate Fcg receptor binding, thus eliminating potential therapy-mediated antibody-dependent enhancement. We administered a cocktail of these three nmAbs to nonhuman primates 1 day before challenge with ZIKV and demonstrated that the nmAbs completely prevented viremia in serum after challenge. Given that numerous antibodies have exceptional safety profiles in humans, the cocktail described here could be rapidly developed to protect uninfected pregnant women and their fetuses ...
TY - JOUR. T1 - Suppression of lipopolysaccharide-induced antiviral transcription factor (STAT1 and NF-kB) complexes by antibody-dependent enhancement of macrophage infection by Ross River virus. AU - Mahalingam, Surendran. AU - Lidbury, Brett. PY - 2002. Y1 - 2002. N2 - Subneutralizing concentrations of antibody may enhance virus infection by bringing the virus-antibody complex into contact with the cell surface Fc receptors; this interaction facilitates entry of virus into the cell and is referred to as antibody-dependent enhancement (ADE) of infection. Northern analysis of macrophage RNA demonstrated that ADE infection by the indigenous Australian alphavirus Ross River (RRV-ADE) ablated or diminished message for tumor necrosis factor alpha (TNF-alpha), nitric-oxide synthase 2 (NOS2), and IFN regulatory factor 1 (IRF-1), as well as for IFN-inducible protein 10 (IP-10) and IFN-beta; the transcription of a control gene was unaffected. Additionally, electrophoretic mobility-shift assay (EMSA) ...
대전광역시 유성구 대학로 245 한국과학기술정보연구원TEL : 042.869.1234 서울시 동대문구 회기로 66NDSL고객센터 : 080.969.4114E-mail : [email protected] 대표자 : 한선화사업자등록번호 : 205-82-04043 ...
Current Research and Scholarly Interests Studies in our lab are aimed at defining mechanisms in human immunity and disease. We are particularly interested the hypothesis that IgG repertoire diversity is a central driver of heterogeneity in human immune functioning and susceptibility to infectious diseases. Our work is defining how diversity that exists in the IgG Fc domain repertoire among people, which we define by serum IgG subclass and Fc glycoform distributions, impacts immune processes such as vaccine responses and susceptibility to antibody-dependent enhancement of dengue disease (Wang TT, Cell. 2015 and Wang TT, Science. 2017). IgG subclass and Fc glycoform distributions are key regulators of immunity because these determine the structure of Fc domains within immune complexes that form during vaccination or infection. Fc structure, in turn, determines the affinity of immune complexes for various Fc receptors on effector cells. Thus, we are studying how the Fc domain repertoire of an ...
Current Research and Scholarly Interests Studies in our lab are aimed at defining mechanisms in human immunity and disease. We are particularly interested the hypothesis that IgG repertoire diversity is a central driver of heterogeneity in human immune functioning and susceptibility to infectious diseases. Our work is defining how diversity that exists in the IgG Fc domain repertoire among people, which we define by serum IgG subclass and Fc glycoform distributions, impacts immune processes such as vaccine responses and susceptibility to antibody-dependent enhancement of dengue disease (Wang TT, Cell. 2015 and Wang TT, Science. 2017). IgG subclass and Fc glycoform distributions are key regulators of immunity because these determine the structure of Fc domains within immune complexes that form during vaccination or infection. Fc structure, in turn, determines the affinity of immune complexes for various Fc receptors on effector cells. Thus, we are studying how the Fc domain repertoire of an ...
Brazilian soldiers last year led a battle against Zika in a door-to-door campaign about how to control mosquitoes that carry the disease. EVARISTO SA/AFP/GETTY IMAGES. CLICK HERE - Science - Enhancement of Zika virus pathogenesis by preexisting antiflavivirus immunity. sciencemag.org - by Jon Cohen - March 30, 2017. Close relatives have complicated relationships with each other even in the viral world. A new mouse study shows that if the animals have antibodies from dengue or West Nile virus, it sets them up for more severe disease from their close cousin, Zika virus.. If such "antibody-dependent enhancement" (ADE) also takes place in people, it could have helped fuel Zikas recent explosion in Brazil, where more than 90% of people in some communities have been infected with dengue. ADE could also complicate the development of vaccines for West Nile, dengue, and Zika. And with the onset of spring reigniting local transmission of Zika last week in the continental United States-where West Nile is ...
Antibody‐dependent cell‐mediated cytotoxicity (ADCC) is an immunologic cytotoxic effector mechanism that is dependent on the cooperative interaction of humoral and cellular effector elements
Patient advocate group Mesh Down Under (MDU), headed by Charlotte Korte, Carmel Berry and Patricia Sullivan, has grown to nearly 500 members since it was founded in 2012. In that time, numerous petitions, communications and meetings with officials from ACC, Medsafe, the Ministry of Health, as well as the Royal Australian and New Zealand College of Obstetricians and Gynaecologists (RANZCOG), the Medical Council, the College of GPs, MPs and even Coleman himself, have taken place. ...
This study proposes to directly test the hypothesis that antibody-dependent enhancement (ADE) is the critical factor in the development of dengue hemorrhagic fever (DHF) in infants. DHF occurs in two distinct clinical settings: a) in children and adults with secondary DENV infection, and b) in infants with primary DENV infection born to mothers with prior DENV infection. The ADE hypothesis proposes that pre-existing serotype-cross-reactive non-neutralizing anti-DENV antibodies bind the heterotypic DENV during secondary infection and enhance its uptake into immune cells, leading to increased viral load and DHF. This model suggests that DHF in DENV-infected infants is caused by the enhancing effect of waning maternal anti-DENV antibodies, thus causing a
Dengue virus co-circulates as four serotypes, and sequential infections with more than one serotype are common. One hypothesis for the increased severity seen in secondary infections is antibody-dependent enhancement (ADE) leading to increased replication in Fc receptor-bearing cells. In this study, we have generated a panel of human monoclonal antibodies to dengue virus. Antibodies to the structural precursor-membrane protein (prM) form a major component of the response. These antibodies are highly cross-reactive among the dengue virus serotypes and, even at high concentrations, do not neutralize infection but potently promote ADE. We propose that the partial cleavage of prM from the viral surface reduces the density of antigen available for viral neutralization, leaving dengue viruses susceptible to ADE by antibody to prM, a finding that has implications for future vaccine design.. ...
The dogma of the antibody-dependent enhancement model (ADE) for the development of dengue hemorrhagic fever (DHF) is being challenged by research published this week in PLoS Medicine.
Nunya Chotiwan was born in Bangkok, Thailand. She received the Sri Trang Thong Scholarship awarded to outstanding students in science, and joined the Honors Program during her undergraduate studies at Mahidol University, Thailand. She graduated with a Bachelors Degree, summa cum laude, in Biology. In 2011-2013, Nunya received a Fulbright Scholarship to pursue a Masters Degree at the Department of Microbiology, Immunology and Pathology (MIP) at Colorado State University. During this period, she conducted research on "molecular determinant of dengue virus envelop protein that are critical for virus entry and fusion in antibody-dependent enhancement of infection" with Dr. Claire Y.-H Huang at the Centers for Disease Control and Prevention. After her graduation, Nunya continued her graduate studies joining the Ph.D. program in the Dept. of MIP at Colorado State University. She received a research assistantship and conducts her Ph.D. dissertation with Dr. Rushika Perera, a Boettcher Investigator at ...
The intricate circuitry formed by amacrine cells in the inner plexiform layer (IPL) of the retina suggests that these interneurons play a major role in shaping the visual message. The majority of amacrine cells in the vertebrate retina are GABAergic. Thus, elucidating how GABAergic signaling is modulated in the IPL is critical in order to understand how the visual message is processed in the retina. The results presented here suggest that GABAergic signaling between amacrine cells can be modulated by the activation of metabotropic glutamate receptor 5 (mGluR5) or by the production of the second messenger, nitric oxide (NO). A novel mGluR5 splice variant was isolated from the chicken retina with a truncated carboxy-terminal tail. Whole cell electrophysiological experiments indicated that activation of mGluR5 enhances GABA-gated currents recorded from cultured chick amacrine cells. This mGluR5-dependent enhancement occurred through the inositol 1,4,5-trisphosphate pathway and was dependent upon ...
Dengue is a global public health threat caused by infection with any of the 4 related dengue virus serotypes (DENV1-4). Clinical manifestations range from self-limiting febrile illness, known as dengue fever (DF), to life-threatening severe diseases, such as dengue hemorrhagic fever (DHF) or dengue shock syndrome (DSS). Most cases of DHF/DSS are associated with secondary heterotypic infections through a phenomenon that is described as antibody-dependent enhancement of infection (ADE). There are an estimated 400 million human infections and several hundred thousand cases of severe dengue occurring yearly. At present, however, there are no approved antiviral drugs against DENV infection. The lack of a suitable animal model has hampered the evaluation of novel antiviral candidates for DENV infection. Since DENV poorly establishes infection in immunocompetent mice, AG129 mice (lacking type I and II IFN [interferon] receptors) and mouse-adapted DENV2 strains have been applied to dengue animal models that
Research recently published in PLoS Medicine challenges the dogma of the antibody-dependent enhancement model (ADE) for the development of dengue hemorrhagic fever (DHF). Dengue virus infection usually causes a severe flu like illness, although symptoms may be mild in young children. DHF, however, is a severe and sometimes fatal complication of dengue virus infection that affects about half a million people every year. DHF patients usually fall into two groups; children and adults who become infected with a second dengue virus serotype after an initial primary dengue virus infection with a different serotype, and infants with primary dengue virus infections born to mothers who have some dengue virus immunity. The current model for development of DHF in infants around 6 months old is that anti-dengue virus antibodies transferred from a dengue-immune mother to her child somehow enhance dengue virus infection, resulting in more severe symptoms (the antibody-dependent enhancement model). A ...
BACKGROUND: Dengue is one of the most important human diseases transmitted by an arthropod vector and the incidence of dengue virus infection has been increasing - over half the worlds population now live in areas at risk of infection. Most infections are asymptomatic, but a subset of patients experience a potentially fatal shock syndrome characterised by plasma leakage. Severe forms of dengue are epidemiologically associated with repeated infection by more than one of the four dengue virus serotypes. Generally attributed to the phenomenon of antibody-dependent enhancement, recent observations indicate that T-cells may also influence disease phenotype. METHODS AND FINDINGS: Virus-specific cytotoxic T lymphocytes (CTL) showing high level cross reactivity between dengue serotypes could be expanded from blood samples taken during the acute phase of secondary dengue infection. These could not be detected in convalescence when only CTL populations demonstrating significant serotype specificity were
Dengue virus is a human pathogen that causes dengue fever, which can either resolve after mild fever or lead to severe dengue hemorrhagic fever/dengue shock syndrome. The role of dengue virus levels in the blood and the kinetics of infection and immune response that results in severe dengue disease in humans is not well characterized. In this study, we analyzed 97 children with varying degrees of dengue disease, and we show that the dengue virus quantity in blood does not show any significant association with severe disease. However, most severe dengue patients had lower levels of interferons and Th1 cytokines and increased levels of secreted factors such as IL-6, IL-8 and IL-10 that could potentially cause leakage in blood capillaries. Our results indicate that monocytes, which are infected with dengue virus in patients, could possibly play a major role in dengue pathogenesis. Furthermore, using computational analysis we identified association of some of the secreted factors with severe disease ...
TY - JOUR. T1 - Prolonged persistence of IgM against dengue virus detected by commonly used commercial assays. AU - Chien, Yu-Wen. AU - Liu, Zi Hu. AU - Tseng, Fan Chen. AU - Ho, Tzu Chuan. AU - Guo, How-Ran. AU - Ko, Nai-Ying. AU - Ko, Wen-Chien. AU - Perng, Guey-Chuen. PY - 2018/4/2. Y1 - 2018/4/2. N2 - Background: Initial symptoms of dengue fever are non-specific, and thus definite diagnosis requires laboratory confirmation. Detection of IgM against dengue virus (DENV) has become widely used for dengue diagnosis. Understanding the persistence of anti-DENV IgM in subjects after acute infection is essential in order to interpret test results correctly. Although the longevity of anti-DENV IgM has been vehemently investigated in symptomatic children, anti-DENV IgM persistence in adults and in asymptomatically infected people have seldom been reported. Methods: We prospectively investigated 44 adults with detectable anti-DENV IgM in a serosurvey conducted in the 2015 dengue epidemic in Tainan, ...
The immunopathology of severe dengue remains incompletely understood. Most patients who develop severe dengue have had prior infection with one or more dengue serotypes. When an individual is infected... more
Dengue virus (DENV) has spread through most tropical and subtropical areas of the world and represents a serious public health problem. The control of DENV infection has not yet been fully successful due to lack of effective therapeutics or vaccines. Nevertheless, a better understanding of the immune responses against DENV infection may reveal new strategies for eliciting and improving antiviral immunity. T cells provide protective immunity against various viral infections by generating effector cells that cooperate to eliminate antigens and memory cells that can survive for long periods with enhanced abilities to control recurring pathogens. Following activation, CD8 T cells can migrate to sites of infection and kill infected cells, whereas CD4 T cells contribute to the elimination of pathogens by trafficking to infected tissues and providing help to innate immune responses, B cells, as well as CD8 T cells. However, it is now evident that CD4 T cells can also perform cytotoxic functions and induce
Build: Wed Jun 21 18:33:50 EDT 2017 (commit: 4a3b2dc). National Center for Advancing Translational Sciences (NCATS), 6701 Democracy Boulevard, Bethesda MD 20892-4874 • 301-435-0888. ...
The Japanese encephalitis virus (JEV) is responsible for zoonotic severe viral encephalitis transmitted by Culex mosquitoes. Although birds are reservoirs, pigs play a role as amplifying hosts, and are affected in particular through reproductive failure. Here, we show that a lentiviral JEV vector, expressing JEV prM and E proteins (TRIP/JEV.prME), but not JEV infection induces strong antibody-dependent enhancement (ADE) activities for infection of macrophages. Such antibodies strongly promoted infection via Fc receptors. ADE was found at both neutralizing and non-neutralizing serum dilutions. Nevertheless, in vivo JEV challenge of pigs demonstrated comparable protection induced by the TRIP/JEV.prME vaccine or heterologous JEV infection. Thus, either ADE antibodies cause no harm in the presence of neutralizing antibodies or may even have protective effects in vivo in pigs. Additionally, we found that both pre-infected and vaccinated pigs were not fully protected as low levels of viral RNA were found in
The cell viability and DNA damage in unstimulated sheep primary lymphocytes subjected to different extremely low electromagnetic field intensities (5, 50 and 100 µT; 50 Hz) were studied with special emphasis on apoptosis. Sheep primary lymphocytes cultured in RPMI, supplemented with 10% FBS in the absence of mitogens, were exposed till 16 h. The cell viability assessment by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay showed a dose dependent enhancement of viability at 16 h. Further, quantitative DNA laddering and flow cytometric analysis showed a significant decrease in apoptosis of the cells subjected to 100 (p
Sonodynamic therapy, the ultrasound dependent enhancement of cytotoxic activities of certain compounds (sonosensitizers) in studies with cells in vitro and in tumor bearing animals, is reviewed. The attractive features of this modality for cancer treatment emerges from the ability to focus the ultrasound energy on malignancy sites buried deep in tissues and to locally activate a preloaded sonosensitizer. Possible mechanisms of sonodynamic therapy include generation of sonosensitizer derived radicals which initiate chain peroxidation of membrane lipids via peroxyl and/or alkoxyl radicals, the physical destabilization of the cell membrane by the sonosensitizer thereby rendering the cell more susceptible to shear forces or ultrasound enhanced drug transport across the cell membrane (sonoporation). Evidence against the role of singlet oxygen in sonodynamic therapy is discussed. The mechanism of sonodynamic therapy is probably not governed by a universal mechanism, but may be influenced by multiple factors
BACKGROUND: Dengue virus is endemic in tropical and sub-tropical resource-poor countries. Dengue illness can range from a nonspecific febrile illness to a severe disease, Dengue Shock Syndrome (DSS), in which patients develop circulatory failure. Earlier diagnosis of severe dengue illnesses would have a substantial impact on the allocation of health resources in endemic countries. METHODS AND FINDINGS: We compared clinical laboratory findings collected within 72 hours of fever onset from a prospective cohort children presenting to one of two hospitals (one urban and one rural) in Thailand. Classification and regression tree analysis was used to develop diagnostic algorithms using different categories of dengue disease severity to distinguish between patients at elevated risk of developing a severe dengue illness and those at low risk. A diagnostic algorithm using WBC count, percent monocytes, platelet count, and hematocrit achieved 97% sensitivity to identify patients who went on to develop DSS while
Infectious Diseases - Dengue / Dengue Hemorrhagic Fever / Dengue Shock Syndrome Support Group - Dengue is the most important mosquito-transmitted viral disease in terms of morbidity and mortality.
In a prospective study in the outpatients department of three large hospitals in Ho Chi Minh City, Viet Nam, we will determine the early diagnostic sensitivity, specificity, positive and negative predictive values of two NS1 diagnostic tests in severe dengue cases.. The study is intended to develop a prognostic algorithm for the early identification of severe dengue cases.. Routine demographic, haematological and biochemical laboratory markers will be utilized to derive a prognostic algorithm that is clinically-useful for guiding patient triage and interventions.. We hope to discover and evaluate new early biomarkers of severe dengue and will evaluate candidate host response molecules and virological markers for their prognostic value.. We further plan to understand the phylogeography of DENV in the super-urban setting of HCMC.. We will use genome scale sequencing of DENV together with geospatial information on the residential addresses of patients to better understand transmission dynamics in ...
In a prospective study in the outpatients department of three large hospitals in Ho Chi Minh City, Viet Nam, we will determine the early diagnostic sensitivity, specificity, positive and negative predictive values of two NS1 diagnostic tests in severe dengue cases.. The study is intended to develop a prognostic algorithm for the early identification of severe dengue cases.. Routine demographic, haematological and biochemical laboratory markers will be utilized to derive a prognostic algorithm that is clinically-useful for guiding patient triage and interventions.. We hope to discover and evaluate new early biomarkers of severe dengue and will evaluate candidate host response molecules and virological markers for their prognostic value.. We further plan to understand the phylogeography of DENV in the super-urban setting of HCMC.. We will use genome scale sequencing of DENV together with geospatial information on the residential addresses of patients to better understand transmission dynamics in ...
La Jolla Institute scientists have proved a hypothesis that said antibodies contribute to severe dengue virus-induced disease.
Dengue fever, caused by infections with the dengue virus (DENV), affects nearly 400 million people globally every year. Early diagnosis and management can reduce the morbidity and mortality rates of severe forms of dengue disease as well as decrease the risk of wider outbreaks. ...
We have identified and characterized nine antigenic epitopes on the E envelope of Japanese encephalitis virus (JEV) by using mAb. Passive administration of most of the anti-JEV mAb protected mice from i.v. challenge with 1.5 x 10(3) plaque-forming units of JEV, JaGAr-01 strain. Some mAb, which possess high neutralization activity in vitro, showed high protection, and JEV-specific N mAb 503 was found the most protective. Even an injection of 2.5 micrograms/mouse of mAb 503 protected all mice from JEV infection. Furthermore, an injection of about 200 micrograms of mAb 503 on day 5 postinfection protected 82% of the mice, even when JEV was detected in more than 85% of the infected mouse brains. Synergism of protection was observed with mixtures of several mAb directed against different epitopes. Although in a murine macrophage cell line, all of the mAb groups showed antibody-dependent enhancement (ADE) of JEV infectivity in vitro, and only two flavivirus cross-reactive mAb groups showed ADE of ...
Purpose: The anaplastic lymphoma kinase (ALK) inhibitor crizotinib has been used in patients with lung cancer or inflammatory myofibroblastic tumor (IMT), both types harboring ALK fusions. However, detection of some ALK fusions is problematic with conventional anti-ALK immunohistochemistry because of their low expression. By using sensitive immunohistochemistry, therefore, we reassessed "ALK-negative" IMT cases defined with conventional immunohistochemistry (approximately 50% of all examined cases).. Experimental Design: Two cases of ALK-negative IMT defined with conventional anti-ALK immunohistochemistry were further analyzed with sensitive immunohistochemistry [the intercalated antibody-enhanced polymer (iAEP) method].. Results: The two "ALK-negative" IMTs were found positive for anti-ALK immunohistochemistry with the iAEP method. 5′-rapid amplification of cDNA ends identified a novel partner of ALK fusion, protein-tyrosine phosphatase, receptor-type, F polypeptide-interacting ...
Programme in Emerging Infectious Diseases, Duke‐NUS Medical School, Singapore, SingaporeDepartment of Microbiology and Immunology, National University of Singapore, Singapore, SingaporeSaw Swee Hock School of Public Health, National University of Singapore, Singapore, SingaporeInfectious Diseases Interdisciplinary Research Group, Singapore MIT Alliance Research and Technology CREATE Campus, Singapore, Singapore ...
The rapid spread of dengue is a worldwide public health problem. In two clinical studies of dengue in Managua, Nicaragua, we observed an abrupt increase in disease severity across several epidemic seasons of dengue virus serotype 2 (DENV-2) transmission. Waning DENV-1 immunity appeared to increase the risk of severe disease in subsequent DENV-2 infections after a period of cross-protection. The increase in severity coincided with replacement of the Asian/American DENV-2 NI-1 clade with a new virus clade, NI-2B. In vitro analyses of viral isolates from the two clades and analysis of viremia in patient blood samples support the emergence of a fitter virus in later, relative to earlier, epidemic seasons. In addition, the NI-1 clade of viruses was more virulent specifically in children who were immune to DENV-1, whereas DENV-3 immunity was associated with more severe disease among NI-2B infections. Our data demonstrate that the complex interaction between viral genetics and population dynamics of ...
1 PengertianDengue Fever (DF) adalah penyakit demam akut selama 2-7 hari dengan dua atau lebih manifestasi berikut: nyeri skepala,nyeri perut, mual, muntah, nyeri retro orbital, myalgia, atralgia, ruam kulit, hepatomegali, manifestasi perdarahan, danlekopenia.Dengue Haemoragic Fever (DHF) adalah penyakit demam akut yang disertai dengan adanya manifestasi perdarahan, yangbertendensi mengakibatkan renjatan yang dapat menyebabkan kematian (Arief Mansjoer &Suprohaita; 2000).Dengue Haemoragic Fever (DHF) adalah infeksi akut yang disebabkan oleh Arbovirus (arthropodborn virus) dan ditularkanmelalui gigitan nyamuk Aedes Aegypti dan Aedes Albopictus. (Ngastiyah, 1995).Dengue Haemoragic Fever (DHF) adalah suatu penyakit infeksi yang disebabkan oleh virus dengue dengan tipe I - IVdengan infestasi klinis dengan 5 - 7 hari disertai gejala perdarahan dan jika timbul tengatan angka kematiannya cukuptinggi (UPF IKA, 1994).Dengue Hemoragik Fever (DHF) adalah kasus demam dengue dengan kecenderungan perdarahan ...
SUPPLEMENT: The four serotypes of dengue virus (DENV) cause 100 million cases of dengue fever (DF) yearly. In ~500,00 cases, infection with DENV results in a severe disease, previously known as dengue hemorrhagic fever (DHF) causing 20,000 annual deaths 1-3. Five percent of cases of DHF affect infants during primary infections 4. Since the 1980s, cases of DHF expanded from SE Asia to other regions 1-3. In Northern Argentina, 25,000 cases of dengue were reported in 2009 5. An important consequence of this problema is that unlike in SE Asia, where ~99% of women of childbearing age are immune to DENV and transfer protective antibody to their babies through the placenta 6, susceptible fetuses and infants in Northern Argentina experience primary DENV infection while lacking maternal antibody to modulate disease. This situation has led to infrequent and severe clinical manifestations in neonates and infants. In recent years, we detected infants with severe congenital DENV infection 7 and immature ...
Dengue fever does not have a specific treatment plan. Symptoms of dehydration, fever, body pains, nausea, and vomiting may be present. These can be treated with rest, plenty of fluids, and Tylenol. However, if you become infected, you should visit a doctor, since conditions and resulting appropriate treatment can vary. Additionally, dengue can manifest in two severe forms, dengue hemorrhagic fever and dengue shock syndrome, which have more serious effects, like bleeding and shock, so getting medical attention is important.. ...
Findings provide promising target for drug and vaccine research This is the schematic of a dengue virus enveloped by proteins. Credit: Used with permission
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Ecological interactions are fundamental to the transmission of infectious disease. Arboviruses are particularly elegant examples, where rich arrays of mechanisms influence transmission between vectors and hosts. Research on host contributions to the ecology of arboviral diseases has been undertaken within multiple subdisciplines, but significant gaps in knowledge remain and multidisciplinary approaches are needed. Through our multidisciplinary review of the literature we have identified five broad areas where hosts may influence the ecology of arboviral transmission: host immunity; cross-protective immunity and antibody-dependent enhancement; host abundance; host diversity; and pathogen spillover and dispersal. Herein we discuss the known and theoretical roles of hosts within these topics and then apply this knowledge to three epidemiologically important mosquito-borne arboviruses that occur in Australia: dengue virus (DENV), Murray Valley encephalitis virus (MVEV), and Ross River virus (RRV). ...
Monocytes that traffic to lymph nodes and the spleen ultimately function in low O2 environments. While low O2 alters FcγR expression transcriptionally (Bosco et al, 2006), it is unknown how FcγR protein levels and other cellular functions that are affected can impact DENV infection. This study thus provides a first molecular view of the contributory role of low‐oxygen environments on cellular functions that impact dengue pathogenesis.. Oxygen level is known to affect viral pathogenesis in different ways. Generally, hypoxia impairs viruses that naturally infect tissues with high atmospheric oxygen concentration such as influenza virus (Magill & Francis, 1936), adenovirus (Pipiya et al, 2005), and simian virus 40 (Riedinger et al, 1999). In contrast, hypoxia has been shown to increase the infection of viruses, such as hepatitis C virus (HCV) (Vassilaki et al, 2013) and sendai virus (Ebbesen et al, 1991), which naturally target organs with low oxygen concentrations such as the liver, spleen, ...
Created by Brandon Kitchens, student of Tyrrell Conway at the University of Oklahoma. 1 Karriem-Norwood, Varnada, MD. "Dengue Fever: Symptoms, Causes, and Treatments." WebMD. WebMD, 20 Sept. 2012. Web. 24 July 2015 2Martina, B. E. E., Koraka, P., & Osterhaus, A. D. M. E. Dengue virus pathogenesis: An integrated view. Clinical Microbiology Reviews 22, 564-581 (2009). doi:10.1128/CMR.00035-09 3"Vector-Borne Viral Infections". World Health Organization. Retrieved 17 January 2011. 4 Retrieved 2010-12-23Center for Disease Control and Prevention. "Chapter 5 - Dengue Fever (DF) and Dengue Hemorrhagic Fever (DHF)". 2010 Yellow Book. 5 Wilder-Smith A, Chen LH, Massad E, Wilson ME (January 2009). "Threat of dengue to blood safety in dengue-endemic countries". Emerging Infect. Dis. 15 (1): 8-11. doi:10.3201/eid1501.071097. PMID 19116042. PMC 2660677. 6 S. B. Halstead (2007) Dengue. Lancet 370, 1644-1652 7 CDC. Centers for Disease Control and Prevention. Centers for Disease Control and Prevention, 09 June ...
The immune mechanisms involved in dengue fever and dengue hemorrhagic/dengue shock syndrome are not well understood. The ex vivo activation status of immune cells during the dengue disease in patients was examined. CD4 and CD8 T cells were reduced during the acute phase. Interestingly, CD8 T cells co-expressing activation marker HLA-DR, Q, P, and cytolytic granule protein-Tia-1 were significantly higher in dengue patients than in controls. Detection of adhesion molecules indicated that in dengue patients the majority of T cells (CD4 and CD8) express the activation/memory phenotype, characterized as CD44HIGH and lack the expression of the naïve cell marker, CD62LLOW. Also, the levels of T cells co-expressing ICAM-1 (CD54), VLA-4, and LFA-1 (CD11a) were significantly increased. CD8 T lymphocytes expressed predominantly low levels of anti-apoptotic molecule Bcl-2 in the acute phase, possibly leading to the exhibition of a phenotype of activated/effector cells. Circulating levels of IL-18, TGF-β1 ...
Dengue fever (DF) and dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS) are considered the most medically important and widespread mosquito-bor...
Synonyms: breakbone fever, dengue haemorrhagic fever, dengue shock syndrome, dandy fever, seven-day fever, duengero, ki denga pepo (Swahili, meaning sudden...
Dengvaxia is for the prevention of dengue disease caused by all dengue virus serotypes in people aged 9 to 16 years who have previously had laboratory-confirmed dengue infection and who live in endemic areas.
Journal of Clinical and Diagnostic Research aims to publish findings of doctors at grass root level and post graduate students, so that all unique medical experiences are recorded in literature.
MAJORITY of 14 children who died of Dengue Shock Syndrome were given the Dengvaxia dengue vaccine, as bared by the Public Attorneys Office (PAO), the Department of Health (DOH) said yesterday.
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It has previously been shown that activation and apoptosis of T cells increase with severity of disease in young DENV-infected patients, a process that is widely considered to be involved in the pathogenesis of dengue (32, 33, 39-43). Similarly, we found that one-third of live B cells in individuals with severe secondary DENV infection were undergoing apoptosis that was positively correlated with B cell proliferation and activation. These data suggest that DENV infection promotes activation-induced B cell death and increased B cell turnover, which is consistent with our finding of homeostatic levels of B cells in the circulation despite severe disease. Activated B cells expressed CD95, which may contribute to apoptosis through engagement of the CD95/CD95L pathway; a similar mechanism has been proposed to account for apoptosis of CD8+ T cells during severe DENV infection (32, 33).. Our finding of massive expansion of plasmablasts during days 4-7 of dengue symptoms in adults in Brazil is in ...
This page includes the following topics and synonyms: Dengue, Break-bone Fever, Dandy Fever, Duengero Fever, Seven-day Fever, Dengue Hemorrhagic Fever, Dengue Fever, Aden Fever, Dengue Shock Syndrome.
Monoclonal antibodies against tumour cells mainly act via antibody dependent cellular cytotoxicity.. They also act via complement mediated lysis and by induction of apoptosis.. ...
(CIDRAP News) - Saying dengue virus infections and deaths have mushroomed in recent years, the World Health Organization (WHO) recently released a strategy report that sets a goal of cutting deaths in half and reducing cases by 25% over the next 8 years.
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Dengue shock syndrome: A syndrome due to the dengue virus that tends to affect children under 10, causing abdominal pain, hemorrhage (bleeding) and circulatory collapse (shock). Known also as dengue hemorrhagic fever (DHF), it starts abruptly with high continuous fever and headache plus respiratory and intestinal symptoms with sore throat, cough, nausea, vomiting, and abdominal pain. Shock occurs after 2 to 6 days with sudden collapse, cool clammy extremities, weak thready pulse, and blueness around the mouth (circumoral cyanosis). There is bleeding with easy bruising, blood spots in the skin (petechiae), spitting up blood (hematemesis), blood in the stool (melena), bleeding gums and nosebleeds (epistaxis). Pneumonia and heart inflammation (myocarditis) may be present. The mortality is appreciable ranging from 6 to 30%. Most deaths occur in children. Infants under a year of age are especially at risk of death. It is also called Philippine or Southeast Asian hemorrhagic fever. ...
Author Summary Severe dengue infections are characterized by thrombocytopenia, clinical bleeding and plasma leakage. Activation of the endothelium, the inner lining of blood vessels, leads to the secretion of storage granules called Weibel Palade bodies (WPBs). We demonstrated that severe dengue in Indonesian children is associated with a strong increase in plasma levels of the WPB constituents von Willebrand factor (VWF), VWF propeptide and osteoprotegerin (OPG). An increased amount of the hemostatic protein VWF was in a hyperreactive, platelet binding conformation, and this was most pronounced in the children who died. VWF levels at enrollment were lower than expected from concurrent VWF propeptide and OPG levels and VWF levels did not correlate well with markers of disease severity. Together, this suggests that VWF is being consumed during severe dengue. Circulating levels of the VWF-cleaving enzyme ADAMTS-13 were reduced. VWF is a multimeric protein and a subset of children had a decrease in large
Everyone knows how mosquitos can wreck an end-of-summer picnic. But in some climates, these pesky intruders persist and carry a variety of detrimental diseases-some with no preventative vaccines or targeted therapies. One such passenger is dengue virus (DENV), which infects up to 400 million people each year and comes in several serotypes (1 to 4) and disease presentations-from mild infection to severe disease and sometimes death. But to treat or prevent dengue requires that we have a more complete picture of the disease pathology. Now, Modhiran et al. and Beatty et al. describe the results of in vitro and in vivo experiments that point to circulating dengue virus non-structural protein 1 (NS1) and the innate immune Toll-like receptor 4 (TLR4) as a focus for basic scientists as well as vaccine and drug developers.. DENV infection protects a patient from future reinfection with the same DENV serotype as well as producing temporary immune protection from severe dengue disease caused by a different ...
New results from an early-stage clinical trial of a dengue vaccine co-developed by researchers at the University of Vermont (UVM), Johns Hopkins University and the National Institutes of Health, bring positive news for the reported 50 to 100 million individuals infected annually with the deadly virus. According to the findings, the vaccine is safe and stimulates a strong immune response in most vaccine recipients.. The study appears in the March 15, 2013 issue of the Journal of Infectious Diseases.. Transmitted to humans by Aedes mosquitoes and prevalent in many tropical and subtropical regions of the world, dengue infection is caused by any of four related viruses: Dengue viruses (DENV) 1 through 4. Classically described dengue fever includes fever, headache, severe joint and muscle pain, and rash. Severe dengue infections, dengue hemorrhagic fever, and dengue shock syndrome include a higher risk of complications and death and are more commonly seen after a second infection with dengue viruses. ...
Dengue is a mosquito-borne disease caused by the dengue viruses. There are four distinct but related virus serotypes all of which can cause dengue fever or the more serious forms of the disease, dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS).
Substantial gaps remain in the basic understanding of the pathogenesis of dengue infection. In large part, this limitation is related to the lack of a suitable animal model. Rhesus monkeys develop viremia similar in pattern to humans after dengue vir
Read about dalazatide (formerly ShK-186), an investigational therapy under development by KPI Therapeutics to treat multiple sclerosis and other disorders.
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Veterinary researchers close to Feline Infectious Peritonitis treatment read more at news.vin.com FIP, or Feline Infectious Peritonitis, is a disease
Feline Infectious Peritonitis or FIP is a fatal viral disease that creates a lot of fear and confusion in shelters. Find out information on the causes, transmissions, symptoms, and treatments.
In the past, expression of a viral protein, the 7b protein, was postulated to occur only with virulent FCoV, and thus some authors have held that cats with antibodies to this protein were likely to have FIP. A presentation by Kennedy (Kennedy MA. Diagnostic methods for feline viral pathogens. Proc. 21st ACVIM Forum, Charlotte, NC, 2003, 733-735) stated that while cats with FIP are consistently antibody positive for this protein, it may also be present in healthy cats ...
Accurate and up to date information on FCoV, the cause of FIP, from Dr Diane Addie, lecturer and researcher in this field based at Glasgow University, UK. ...
Antibody-dependent CD56 T cell responses are functionally impaired in long-term HIV-1 infection. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Dengue Virus 3, 1 mg. The dengue virus is responsible for the tropical and sub-tropical diseases, dengue (DF) and dengue haemorrhagic fever (DHF), transmitted to individuals through the bite of the Aedes mosquito.
BACKGROUND: Severe dengue with severe plasma leakage (SD-SPL) is the most frequent of dengue severe form. Plasma biomarkers for early predictive diagnosis of SD-SPL are required in the primary clinics for the prevention of dengue death. METHODOLOGY: Among 63 confirmed dengue pediatric patients recruited, hospital based longitudinal study detected six SD-SPL and ten dengue with warning sign (DWS). To identify the specific proteins increased or decreased in the SD-SPL plasma obtained 6-48 hours before the shock compared with the DWS, the isobaric tags for relative and absolute quantification (iTRAQ) technology was performed using four patients each group ...
SCAN HISTORY OWL25_2 2 300 NSINGLE SPTR37_9f 2 125 NSINGLE INITIAL MOTIF SETS ADENOVSFIBRE1 Length of motif = 11 Motif number = 1 ADV fibre protein motif I - 1 PCODE ST INT EDDFNPVYPYE FIBP_ADE40 7 7 SDSFNPVYPYE FIBP_ADE07 26 26 EDDFNPVYPYG FIBP_ADE08 8 8 EDDFNPVYPYG FIBP_ADE09 8 8 EDTFNPVYPYD FIBP_ADE05 8 8 STSFNPVYPYE FIBP_ADE03 8 8 PANFDPVYPYD FIBP_ADECG 9 9 PANYDPVYPYD CA2FIBER 9 9 ADENOVSFIBRE2 Length of motif = 13 Motif number = 2 ADV fibre protein motif II - 1 PCODE ST INT DIPFITPPFASSN FIBP_ADE40 23 5 QHPFINPGFISPN FIBP_ADE07 42 5 NIPFLTPPFVSSN FIBP_ADE08 24 5 NIPFLTPPFVSSD FIBP_ADE09 24 5 TVPFLTPPFVSPN FIBP_ADE05 25 6 QHPFINPGFISPD FIBP_ADE03 24 5 PKPSTQPPFFNDR FIBP_ADECG 21 1 PGSSTQPPFFNNK CA2FIBER 21 1 ADENOVSFIBRE3 Length of motif = 15 Motif number = 3 ADV fibre protein motif III - 1 PCODE ST INT NGALTLKLGTGLNID FIBP_ADE40 57 21 GGSLQLKVGGGLTID FIBP_ADE07 76 21 NQNVSLKVGGGLTLQ FIBP_ADE08 58 21 NGNVSLKVGGGLTLQ FIBP_ADE09 58 21 NGMLALKMGNGLSLD FIBP_ADE05 59 21 SGSLQLKVGSGLTVD ...
We lost our dear little friend, Earl Grey yesterday after a brief, but fatal illness. He suddenly came down with FIP (Feline Infectious Peritonitis). We decided not to let him suffer, as the Doctor said that he could not last over two months, and there was no hope for a recovery. We are shocked and saddened by his passing, and are reminded of how fragile life is. Rest in Peace, pretty kitty ...
The baby died at around 7.30 PM and the cause of the babys death was bilateral pneumonia with septic shock in a case of severe Dengue," a senior doctor at the institute said quoting from the death certificate issued.. The baby, a resident of the Kareya area in the city, was admitted at the paediatric institute yesterday after being referred by a nursing home.. ...

Antibody-dependent enhancement - WikipediaAntibody-dependent enhancement - Wikipedia

Gras, GS; Dormont, D (1991). "Antibody-dependent and antibody-independent complement-mediated enhancement of human ... or antibody-dependent cellular cytotoxicity-mediating antibodies (ADCC) in the serum contains infection enhancing antibodies( ... Antibody-dependent enhancement (ADE) occurs when non-neutralising antiviral proteins facilitate virus entry into host cells, ... Takada, A; Feldmann, H; Ksiazek, TG; Kawaoka, Y (2003). "Antibody-Dependent Enhancement of Ebola Virus Infection". Journal of ...
more infohttps://en.wikipedia.org/wiki/Antibody-dependent_enhancement

Determination of antibody concentration as the main parameter in a dengue virus antibody-dependent enhancement assay using FcγR...Determination of antibody concentration as the main parameter in a dengue virus antibody-dependent enhancement assay using FcγR...

"Determination of antibody concentration as the main parameter in a dengue virus antibody-dependent enhancement assay using FcγR ... Determination of antibody concentration as the main parameter in a dengue virus antibody-dependent enhancement assay using FcγR ... Determination of antibody concentration as the main parameter in a dengue virus antibody-dependent enhancement assay using FcγR ... Antibody-dependent enhancement of dengue virus infection mediated by bispecific antibodies against cell surface molecules other ...
more infohttps://www.deepdyve.com/lp/springer_journal/determination-of-antibody-concentration-as-the-main-parameter-in-a-6VUO89P8As

Manipulation of immunodominant dengue virus E protein epitopes reduces potential antibody-dependent enhancement | Virology...Manipulation of immunodominant dengue virus E protein epitopes reduces potential antibody-dependent enhancement | Virology...

Dengue virus Vaccine Antibody-dependent enhancement Dengue hemorrhagic fever Cross-reactive antibody Immune refocusing ... In vitro antibody-dependent enhancement. Heat inactivated mouse sera were pooled, diluted, and titrated. DENV (DENV-1 56BC94/95 ... Littaua R, Kurane I, Ennis FA: Human IgG Fc receptor II mediates antibody-dependent enhancement of dengue virus infection. J ... Morens DM, Halstead SB, Marchette NJ: Profiles of antibody-dependent enhancement of dengue virus type 2 infection. Microb ...
more infohttps://virologyj.biomedcentral.com/articles/10.1186/1743-422X-9-115

west nile virus Protocols and Video...'west nile virus' Protocols and Video...

A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus ... A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus ...
more infohttps://www.jove.com/keyword/west+nile+virus

Antibody-dependent enhancement of severe dengue disease in humans | ScienceAntibody-dependent enhancement of severe dengue disease in humans | Science

They confirmed that antibody-dependent enhancement of disease occurs at a specific range of antibody concentrations. Low levels ... Antibody-dependent enhancement of severe dengue disease in humans. By Leah C. Katzelnick, Lionel Gresh, M. Elizabeth Halloran, ... Antibody-dependent enhancement of severe dengue disease in humans. By Leah C. Katzelnick, Lionel Gresh, M. Elizabeth Halloran, ... Antibody-dependent enhancement of severe dengue disease in humans Message Subject. (Your Name) has forwarded a page to you from ...
more infohttp://science.sciencemag.org/content/358/6365/929

Antibody-Dependent Enhancement of Ebola Virus Infection | Journal of VirologyAntibody-Dependent Enhancement of Ebola Virus Infection | Journal of Virology

In vitro antibody-dependent enhancement assays are insensitive indicators of in vivo vaccine enhancement of equine infectious ... Monoclonal antibodies to the spike protein of feline infectious peritonitis virus mediate antibody-dependent enhancement of ... Antibody-Dependent Enhancement of Ebola Virus Infection. Ayato Takada, Heinz Feldmann, Thomas G. Ksiazek, Yoshihiro Kawaoka ... Antibody-Dependent Enhancement of Ebola Virus Infection Message Subject (Your Name) has forwarded a page to you from Journal of ...
more infohttps://jvi.asm.org/content/77/13/7539.full

Antibody-dependent enhancement of viral infection: molecular mechanisms and in vivo implications.  - PubMed - NCBIAntibody-dependent enhancement of viral infection: molecular mechanisms and in vivo implications. - PubMed - NCBI

... known as antibody-dependent enhancement (ADE) of viral infection, depends on the cross-linking of complexes of virus-antibody ... Antibody-dependent enhancement of viral infection: molecular mechanisms and in vivo implications.. Takada A1, Kawaoka Y. ... Besides the common receptor/coreceptor-dependent mechanism of cellular attachment, some viruses rely on antiviral antibodies ... antibody-mediated modulation of the interaction between viral protein and its coreceptor (human immunodeficiency virus) and ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/14625886?dopt=Abstract

Ligation of Fc gamma receptor IIB inhibits antibody-dependent enhancement of dengue virus infection.  - PubMed - NCBILigation of Fc gamma receptor IIB inhibits antibody-dependent enhancement of dengue virus infection. - PubMed - NCBI

Ligation of Fc gamma receptor IIB inhibits antibody-dependent enhancement of dengue virus infection.. Chan KR1, Zhang SL, Tan ... Ligation of Fc gamma receptor IIB inhibits antibody-dependent enhancement of dengue virus infection ... Ligation of Fc gamma receptor IIB inhibits antibody-dependent enhancement of dengue virus infection ... Ligation of Fc gamma receptor IIB inhibits antibody-dependent enhancement of dengue virus infection ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/21746897

Supplement to Ligation of Fc gamma receptor IIB inhibits antibody-dependent enhancement of dengue virus infection | PNASSupplement to Ligation of Fc gamma receptor IIB inhibits antibody-dependent enhancement of dengue virus infection | PNAS

Ligation of Fc gamma receptor IIB inhibits antibody-dependent enhancement of dengue virus infection. Kuan Rong Chan, Summer Li- ...
more infohttps://www.pnas.org/content/suppl/2011/07/07/1106568108.DCSupplemental

Human IgG Fc receptor II mediates antibody-dependent enhancement of dengue virus infection. | The Journal of ImmunologyHuman IgG Fc receptor II mediates antibody-dependent enhancement of dengue virus infection. | The Journal of Immunology

... and this phenomenon is called antibody-dependent enhancement. This is caused by the uptake of dengue virus-antibody complexes ... Human IgG Fc receptor II mediates antibody-dependent enhancement of dengue virus infection.. R Littaua, I Kurane and F A Ennis ... Based on these results we conclude that Fc gamma RII mediate antibody-dependent enhancement of dengue virus infection in ... Human IgG Fc receptor II mediates antibody-dependent enhancement of dengue virus infection. ...
more infohttp://www.jimmunol.org/content/144/8/3183?ijkey=3626da5d02eb126173dea74b4289a073b67027e8&keytype2=tf_ipsecsha

Role of Dendritic Cells in Antibody-Dependent Enhancement of Dengue Virus Infection | Journal of VirologyRole of Dendritic Cells in Antibody-Dependent Enhancement of Dengue Virus Infection | Journal of Virology

Measurement of antibody-dependent infection enhancement of four dengue virus serotypes by monoclonal and polyclonal antibodies ... Antibody-dependent enhancement of dengue virus growth in human monocytes as a risk factor for dengue hemorrhagic fever. Am. J. ... Role of Dendritic Cells in Antibody-Dependent Enhancement of Dengue Virus Infection. Kobporn Boonnak, Bonnie M. Slike, Timothy ... Antibody-dependent enhancement of infection: bacteria do it too. Trends Immunol. 24:465-467. ...
more infohttps://jvi.asm.org/content/82/8/3939

Antibody dependent enhancement infection of Enterovirus 71 in vitro and in vivo | Virology Journal | Full TextAntibody dependent enhancement infection of Enterovirus 71 in vitro and in vivo | Virology Journal | Full Text

Antibody dependent enhancement (ADE) infection has been reported in various viruses and has been shown to contribute to disease ... In this study, the presence of sub-neutralizing antibody was demonstrated to enhance EV71 infection in THP-1 cells and increase ... and raise practical issues of vaccine development and antibody-based therapy. ... Antibody-dependent enhancement (ADE) of virus infection is a phenomenon in which preexisting sub-neutralizing antibodies ...
more infohttps://virologyj.biomedcentral.com/articles/10.1186/1743-422X-8-106

Suppression of lipopolysaccharide-induced antiviral transcription factor (STAT-1 and NF-kappa B) complexes by antibody...Suppression of lipopolysaccharide-induced antiviral transcription factor (STAT-1 and NF-kappa B) complexes by antibody...

... this interaction facilitates entry of virus into the cell and is referred to as antibody-dependent enhancement (ADE) of ... Subneutralizing concentrations of antibody may enhance virus infection by bringing the virus-antibody complex into contact with ... transcription factor complexes was shown to depend on replicating virus and was not simply a result of general antibody-Fc- ... Molecular mechanisms involved in antibody-dependent enhancement of dengue virus infection in humans.. Jacky Flipse, Jan C. ...
more infohttps://www.semanticscholar.org/paper/Suppression-of-lipopolysaccharide-induced-antiviral-Mahalingam-Lidbury/03ff79f01621588c38ca32818d8418413ee39866

Epitopes required for antibody-dependent enhancement of Ebola virus infection<...Epitopes required for antibody-dependent enhancement of Ebola virus infection<...

"Epitopes required for antibody-dependent enhancement of Ebola virus infection",. abstract = "We have shown that antibody- ... Epitopes required for antibody-dependent enhancement of Ebola virus infection. In: Journal of Infectious Diseases. 2007 ; Vol. ... Epitopes required for antibody-dependent enhancement of Ebola virus infection. Ayato Takada, Hideki Ebihara, Heinz Feldmann, ... Takada A, Ebihara H, Feldmann H, Geisbert T, Kawaoka Y. Epitopes required for antibody-dependent enhancement of Ebola virus ...
more infohttps://researchexperts.utmb.edu/en/publications/epitopes-required-for-antibody-dependent-enhancement-of-ebola-vir

Absence of antibody-dependent enhancement (ADE) of viral infectivity in the epidemic neuropathy in Cuba. 
    		
    		
    	Absence of antibody-dependent enhancement (ADE) of viral infectivity in the epidemic neuropathy in Cuba.

Absence of antibody-dependent enhancement (ADE) of viral infectivity in the epidemic neuropathy in Cuba. Guzmá 관련메뉴. ; n, M G ...
more infohttp://www.ndsl.kr/ndsl/search/detail/article/articleSearchResultDetail.do?cn=NART00103122

Suppression of lipopolysaccharide-induced antiviral transcription factor (STAT1 and NF-kB) complexes by antibody-dependent...Suppression of lipopolysaccharide-induced antiviral transcription factor (STAT1 and NF-kB) complexes by antibody-dependent...

... complexes by antibody-dependent enhancement of macrophage infection by Ross River virus. Proceedings of the National Academy of ... complexes by antibody-dependent enhancement of macrophage infection by Ross River virus. Proceedings of the National Academy of ... complexes by antibody-dependent enhancement of macrophage infection by Ross River virus. In: Proceedings of the National ... complexes by antibody-dependent enhancement of macrophage infection by Ross River virus, Proceedings of the National Academy ...
more infohttps://researchprofiles.canberra.edu.au/en/publications/suppression-of-lipopolysaccharide-induced-antiviral-transcription

Antibody-dependent enhancement of human immunodeficiency virus type 1 (HIV-1) infection in vitro by serum from HIV-1-infected...Antibody-dependent enhancement of human immunodeficiency virus type 1 (HIV-1) infection in vitro by serum from HIV-1-infected...

Antibody-dependent enhancement of human immunodeficiency virus type 1 (HIV-1) infection in vitro by serum from HIV-1-infected ... Antibody-dependent enhancement of human immunodeficiency virus type 1 (HIV-1) infection in vitro by serum from HIV-1-infected ... Based on recent reports of antibody-dependent enhancement of human immunodeficiency virus type 1 (HIV-1) infection in vitro by ... determine whether current HIV-1 candidate vaccines induce production of antibodies that mediate antibody-dependent enhancement ...
more infohttps://scholars.duke.edu/display/pub788982

Molecular determinants of dengue virus 2 envelope protein important for virus entry in FcγRIIA-mediated antibody-dependent...Molecular determinants of dengue virus 2 envelope protein important for virus entry in FcγRIIA-mediated antibody-dependent...

Antibody-dependent enhancement (ADE) of infection may cause severe illness in patients suffering a secondary infection by a ... determinants of dengue virus 2 envelope protein important for virus entry in FcγRIIA-mediated antibody-dependent enhancement of ... Determinants of Dengue Virus 2 Envelope Protein Important for Virus Entry in FcγRIIA-Mediated Antibody-Dependent Enhancement of ... Determinants of Dengue Virus 2 Envelope Protein Important for Virus Entry in FcγRIIA-Mediated Antibody-Dependent Enhancement of ...
more infohttps://phgkb.cdc.gov/PHGKB/phgHome.action?action=forward&dbsource=cdcpub&id=1722

Enhancement of Antibody-Dependent Cellular Cytotoxicity of Neonatal Cells by Interleukin-2 (IL-2) and IL-12 | Clinical and...Enhancement of Antibody-Dependent Cellular Cytotoxicity of Neonatal Cells by Interleukin-2 (IL-2) and IL-12 | Clinical and...

Enhancement of Antibody-Dependent Cellular Cytotoxicity of Neonatal Cells by Interleukin-2 (IL-2) and IL-12. Quoc H. Nguyen, ... Enhancement of Antibody-Dependent Cellular Cytotoxicity of Neonatal Cells by Interleukin-2 (IL-2) and IL-12 ... Enhancement of Antibody-Dependent Cellular Cytotoxicity of Neonatal Cells by Interleukin-2 (IL-2) and IL-12 ... Enhancement of Antibody-Dependent Cellular Cytotoxicity of Neonatal Cells by Interleukin-2 (IL-2) and IL-12 ...
more infohttps://cvi.asm.org/content/5/1/98

IgG2a-mediated enhancement of antibody responses is dependent on FcRgamma+ bone marrow-derived cellsIgG2a-mediated enhancement of antibody responses is dependent on FcRgamma+ bone marrow-derived cells

... Díaz de Ståhl, T Uppsala ... This thesis deals with the in vivo regulatory properties of antibodies and their specific Fc receptors. ... Circulating immune complexes play an important role in the modulation of antibody responses and in the pathogenesis of immune ... Immunization with soluble antigens in complex with specific IgG leads to an augmentation of antibody production. The cellular ...
more infohttp://uu.diva-portal.org/smash/record.jsf?pid=diva2:161136

Stalking influenza by vaccination with pre-fusion headless HA mini-stem | Scientific ReportsStalking influenza by vaccination with pre-fusion headless HA mini-stem | Scientific Reports

... yet they do not have antibody-dependent enhancement activity. ... Furthermore, antibodies indudced by these HA stems have broad ... Passive transfer of immune serum demonstrates the protection is mediated by stem-specific antibodies. ... HA stem-directed broadly neutralizing antibodies with high affinity. Mice vaccinated with the group 1 HA mini-stems are ... report the design of a bacterially expressed polypeptide that mimics a H5 HA stem by protein minimization to focus the antibody ...
more infohttps://www.nature.com/articles/srep22666?error=cookies_not_supported&code=97075c3e-292b-4902-802c-8ef87eb422dc

February - 2010 - ervFebruary - 2010 - erv

When Good Antibodies Go Bad: Antibody-Dependent Enhancement. Posted by ERV on February 19, 2010 ... antibodies even make nice cancer therapies. One of the great things about antibodies is that the cells that make them? They ... Antibodies are normally a good thing. Neutralize viruses and bacterial toxins, tag bacteria for complement so they get blown up ... No meaningful anti-XMRV antibodies. Still no XMRV in the Europe. Longer "Absence… ...
more infohttp://scienceblogs.com/erv/2010/02/

Biology-OnlineBiology-Online

Rethinking the Antibody-Dependent Enhancement Dengue Hemorrhagic Fever Model. Rethinking the Antibody-Dependent Enhancement ... Research recently published in PLoS Medicine challenges the dogma of the antibody-dependent enhancement model (ADE) for the ... antibody-dependent enhancement model). A prospective nested case-control study of infant dengue carried out by Daniel Libraty ... The current model for development of DHF in infants around 6 months old is that anti-dengue virus antibodies transferred from a ...
more infohttps://www.biology-online.org/articles/rethinking-antibody-dependent-enhancement-dengue-hemorrhagic.html

Table of Contents - November 17, 2017, 358 (6365) | ScienceTable of Contents - November 17, 2017, 358 (6365) | Science

Antibody-dependent enhancement of severe dengue disease in humans. By Leah C. Katzelnick, Lionel Gresh, M. Elizabeth Halloran, ... A long-term Nicaraguan pediatric cohort reveals that a narrow range of preexisting antibody titers increases the risk of severe ...
more infohttp://science.sciencemag.org/content/358/6365

AFM Task Force Report | Board of Scientific Counselors | CDCAFM Task Force Report | Board of Scientific Counselors | CDC

Diagnostic (intrathecal), pathogenic antibodies (autoantibodies). *Antibody-dependent enhancement. Support for broad approaches ... Support for measuring antibody response to infection in serum and CSF. * ... Kinetics of disease suggest that antibody-mediated pathology is unlikely. *Low priority given to measuring autoantibody ...
more infohttps://www.cdc.gov/ddid/bsc/afm-report.html
  • This could be due to the preferential survival of long-lived memory B cells producing homotypic antibodies. (wikipedia.org)
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