Antibodies
Protozoan Infections
Antibody Specificity
Antibody Formation
Antibodies, Neutralizing
Eukaryota
One of the three domains of life (the others being BACTERIA and ARCHAEA), also called Eukarya. These are organisms whose cells are enclosed in membranes and possess a nucleus. They comprise almost all multicellular and many unicellular organisms, and are traditionally divided into groups (sometimes called kingdoms) including ANIMALS; PLANTS; FUNGI; and various algae and other taxa that were previously part of the old kingdom Protista.
Antibody Affinity
A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes.
Ciliophora
Fluorescent Antibody Technique
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Antibodies, Anti-Idiotypic
RNA, Protozoan
Binding Sites, Antibody
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Antibodies, Neoplasm
Antibodies, Antinuclear
Autoantibodies directed against various nuclear antigens including DNA, RNA, histones, acidic nuclear proteins, or complexes of these molecular elements. Antinuclear antibodies are found in systemic autoimmune diseases including systemic lupus erythematosus, Sjogren's syndrome, scleroderma, polymyositis, and mixed connective tissue disease.
Protozoan Infections, Animal
Cross Reactions
Amino Acid Sequence
Entamoeba histolytica
Immunoglobulin M
Autoantibodies
Antigens, Protozoan
Neutralization Tests
The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).
Trypanosoma cruzi
Giardia lamblia
Toxoplasma
Antigen-Antibody Reactions
Antibodies, Bispecific
Antibodies, often monoclonal, in which the two antigen-binding sites are specific for separate ANTIGENIC DETERMINANTS. They are artificial antibodies produced by chemical crosslinking, fusion of HYBRIDOMA cells, or by molecular genetic techniques. They function as the main mediators of targeted cellular cytotoxicity and have been shown to be efficient in the targeting of drugs, toxins, radiolabeled haptens, and effector cells to diseased tissue, primarily tumors.
Leishmania
A genus of flagellate protozoa comprising several species that are pathogenic for humans. Organisms of this genus have an amastigote and a promastigote stage in their life cycles. As a result of enzymatic studies this single genus has been divided into two subgenera: Leishmania leishmania and Leishmania viannia. Species within the Leishmania leishmania subgenus include: L. aethiopica, L. arabica, L. donovani, L. enrietti, L. gerbilli, L. hertigi, L. infantum, L. major, L. mexicana, and L. tropica. The following species are those that compose the Leishmania viannia subgenus: L. braziliensis, L. guyanensis, L. lainsoni, L. naiffi, and L. shawi.
Single-Chain Antibodies
Antibodies, Blocking
Antibodies that inhibit the reaction between ANTIGEN and other antibodies or sensitized T-LYMPHOCYTES (e.g., antibodies of the IMMUNOGLOBULIN G class that compete with IGE antibodies for antigen, thereby blocking an allergic response). Blocking antibodies that bind tumors and prevent destruction of tumor cells by CYTOTOXIC T-LYMPHOCYTES have also been called enhancing antibodies. (Rosen et al., Dictionary of Immunology, 1989)
Immunoglobulin G
Antigen-Antibody Complex
Immunoglobulin Fab Fragments
Univalent antigen-binding fragments composed of one entire IMMUNOGLOBULIN LIGHT CHAIN and the amino terminal end of one of the IMMUNOGLOBULIN HEAVY CHAINS from the hinge region, linked to each other by disulfide bonds. Fab contains the IMMUNOGLOBULIN VARIABLE REGIONS, which are part of the antigen-binding site, and the first IMMUNOGLOBULIN CONSTANT REGIONS. This fragment can be obtained by digestion of immunoglobulins with the proteolytic enzyme PAPAIN.
Alveolata
Trypanosoma
Antibodies, Heterophile
Antibodies elicited in a different species from which the antigen originated. These antibodies are directed against a wide variety of interspecies-specific antigens, the best known of which are Forssman, Hanganutziu-Deicher (H-D), and Paul-Bunnell (P-B). Incidence of antibodies to these antigens--i.e., the phenomenon of heterophile antibody response--is useful in the serodiagnosis, pathogenesis, and prognosis of infection and latent infectious states as well as in cancer classification.
Apicomplexa
Antibodies, Catalytic
Trypanosoma brucei brucei
Kinetoplastida
Fluorescent Antibody Technique, Indirect
A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
Rabbits
Intestinal Diseases, Parasitic
Immunoglobulin A
Base Sequence
Immune Sera
Antibodies, Monoclonal, Humanized
Antibodies from non-human species whose protein sequences have been modified to make them nearly identical with human antibodies. If the constant region and part of the variable region are replaced, they are called humanized. If only the constant region is modified they are called chimeric. INN names for humanized antibodies end in -zumab.
Trypanosomatina
A suborder of monoflagellate parasitic protozoa that lives in the blood and tissues of man and animals. Representative genera include: Blastocrithidia, Leptomonas, CRITHIDIA, Herpetomonas, LEISHMANIA, Phytomonas, and TRYPANOSOMA. Species of this suborder may exist in two or more morphologic stages formerly named after genera exemplifying these forms - amastigote (LEISHMANIA), choanomastigote (CRITHIDIA), promastigote (Leptomonas), opisthomastigote (Herpetomonas), epimastigote (Blastocrithidia), and trypomastigote (TRYPANOSOMA).
Hybridomas
Trichomonas
Immunization
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
Epitope Mapping
Protozoan Vaccines
Crithidia
Antibodies, Antiphospholipid
Parasites
Cattle
Hartmannella
Trichomonas vaginalis
Enzyme-Linked Immunosorbent Assay
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Leishmania major
Antigens, Surface
Rumen
The first stomach of ruminants. It lies on the left side of the body, occupying the whole of the left side of the abdomen and even stretching across the median plane of the body to the right side. It is capacious, divided into an upper and a lower sac, each of which has a blind sac at its posterior extremity. The rumen is lined by mucous membrane containing no digestive glands, but mucus-secreting glands are present in large numbers. Coarse, partially chewed food is stored and churned in the rumen until the animal finds circumstances convenient for rumination. When this occurs, little balls of food are regurgitated through the esophagus into the mouth, and are subjected to a second more thorough mastication, swallowed, and passed on into other parts of the compound stomach. (From Black's Veterinary Dictionary, 17th ed)
Entamoeba
Immunoenzyme Techniques
Blotting, Western
Electrophoresis, Polyacrylamide Gel
Coccidiosis
Cells, Cultured
Giardiasis
Immunization, Passive
Immunoblotting
Immunohistochemistry
Leishmania donovani
Immunoassay
Immunoglobulin Fragments
Acanthamoeba
Cryptosporidium parvum
Cryptosporidium
Cloning, Molecular
Species Specificity
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Host-Parasite Interactions
Seroepidemiologic Studies
B-Lymphocytes
Radioimmunoassay
Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.
T-Lymphocytes
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
Sarcocystosis
Amoebozoa
Complement Fixation Tests
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
Leishmania mexicana
A parasitic hemoflagellate of the subgenus Leishmania leishmania that infects man and animals including rodents. The Leishmania mexicana complex causes both cutaneous (LEISHMANIASIS, CUTANEOUS) and diffuse cutaneous leishmaniasis (LEISHMANIASIS, DIFFUSE CUTANEOUS) and includes the subspecies amazonensis, garnhami, mexicana, pifanoi, and venezuelensis. L. m. mexicana causes chiclero ulcer, a form of cutaneous leishmaniasis (LEISHMANIASIS, CUTANEOUS) in the New World. The sandfly, Lutzomyia, appears to be the vector.
Cryptosporidiosis
Hemagglutination Tests
Hemagglutination Inhibition Tests
Antibodies, Antineutrophil Cytoplasmic
Autoantibodies directed against cytoplasmic constituents of POLYMORPHONUCLEAR LEUKOCYTES and/or MONOCYTES. They are used as specific markers for GRANULOMATOSIS WITH POLYANGIITIS and other diseases, though their pathophysiological role is not clear. ANCA are routinely detected by indirect immunofluorescence with three different patterns: c-ANCA (cytoplasmic), p-ANCA (perinuclear), and atypical ANCA.
Spores, Protozoan
Immunoglobulin Variable Region
That region of the immunoglobulin molecule that varies in its amino acid sequence and composition, and comprises the binding site for a specific antigen. It is located at the N-terminus of the Fab fragment of the immunoglobulin. It includes hypervariable regions (COMPLEMENTARITY DETERMINING REGIONS) and framework regions.
Sensitivity and Specificity
Blastocystis hominis
A species of parasitic protozoa found in the intestines of humans and other primates. It was classified as a yeast in 1912. Over the years, questions arose about this designation. In 1967, many physiological and morphological B. hominis characteristics were reported that fit a protozoan classification. Since that time, other papers have corroborated this work and the organism is now recognized as a protozoan parasite of humans causing intestinal disease with potentially disabling symptoms.
Microscopy, Electron
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Legionella pneumophila
Flow Cytometry
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Immunoglobulin Idiotypes
Unique genetically-controlled determinants present on ANTIBODIES whose specificity is limited to a single group of proteins (e.g., another antibody molecule or an individual myeloma protein). The idiotype appears to represent the antigenicity of the antigen-binding site of the antibody and to be genetically codetermined with it. The idiotypic determinants have been precisely located to the IMMUNOGLOBULIN VARIABLE REGION of both immunoglobin polypeptide chains.
Chagas Disease
Infection with the protozoan parasite TRYPANOSOMA CRUZI, a form of TRYPANOSOMIASIS endemic in Central and South America. It is named after the Brazilian physician Carlos Chagas, who discovered the parasite. Infection by the parasite (positive serologic result only) is distinguished from the clinical manifestations that develop years later, such as destruction of PARASYMPATHETIC GANGLIA; CHAGAS CARDIOMYOPATHY; and dysfunction of the ESOPHAGUS or COLON.
Immunologic Techniques
Glycoproteins
Antigens, Neoplasm
Theileria parva
Eimeria
Sequence Homology, Amino Acid
Immunosorbent Techniques
Haptens
Antibody Diversity
The phenomenon of immense variability characteristic of ANTIBODIES. It enables the IMMUNE SYSTEM to react specifically against the essentially unlimited kinds of ANTIGENS it encounters. Antibody diversity is accounted for by three main theories: (1) the Germ Line Theory, which holds that each antibody-producing cell has genes coding for all possible antibody specificities, but expresses only the one stimulated by antigen; (2) the Somatic Mutation Theory, which holds that antibody-producing cells contain only a few genes, which produce antibody diversity by mutation; and (3) the Gene Rearrangement Theory, which holds that antibody diversity is generated by the rearrangement of IMMUNOGLOBULIN VARIABLE REGION gene segments during the differentiation of the ANTIBODY-PRODUCING CELLS.
Recombinant Fusion Proteins
Helminths
Feces
Sarcocystis
Peptide Library
Vaccination
Acanthamoeba castellanii
Protein Binding
Sheep
Hepatitis C Antibodies
Peptides
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Isoantibodies
Parasitic Diseases
Immunoglobulin Isotypes
Immunoglobulins
Multi-subunit proteins which function in IMMUNITY. They are produced by B LYMPHOCYTES from the IMMUNOGLOBULIN GENES. They are comprised of two heavy (IMMUNOGLOBULIN HEAVY CHAINS) and two light chains (IMMUNOGLOBULIN LIGHT CHAINS) with additional ancillary polypeptide chains depending on their isoforms. The variety of isoforms include monomeric or polymeric forms, and transmembrane forms (B-CELL ANTIGEN RECEPTORS) or secreted forms (ANTIBODIES). They are divided by the amino acid sequence of their heavy chains into five classes (IMMUNOGLOBULIN A; IMMUNOGLOBULIN D; IMMUNOGLOBULIN E; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M) and various subclasses.
Entamoebiasis
Membrane Proteins
Peptide Fragments
Antibodies, Monoclonal, Murine-Derived
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Sequence Alignment
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Hepatitis B Antibodies
Immunodiffusion
RNA, Ribosomal, 18S
Precipitin Tests
Binding, Competitive
Neospora
A genus of protozoan parasites of the subclass COCCIDIA. Its species are parasitic in dogs, cattle, goats, and sheep, among others. N. caninum, a species that mainly infects dogs, is intracellular in neural and other cells of the body, multiplies by endodyogeny, has no parasitophorous vacuole, and has numerous rhoptries. It is known to cause lesions in many tissues, especially the brain and spinal cord as well as abortion in the expectant mother.
Macrophages
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
Immunity, Maternally-Acquired
Leishmania infantum
A parasitic hemoflagellate of the subgenus Leishmania leishmania that infects man and animals and causes visceral leishmaniasis (LEISHMANIASIS, VISCERAL). Human infections are confined almost entirely to children. This parasite is commonly seen in dogs, other Canidae, and porcupines with humans considered only an accidental host. Transmission is by Phlebotomus sandflies.
Cell Membrane
Complement System Proteins
Serum glycoproteins participating in the host defense mechanism of COMPLEMENT ACTIVATION that creates the COMPLEMENT MEMBRANE ATTACK COMPLEX. Included are glycoproteins in the various pathways of complement activation (CLASSICAL COMPLEMENT PATHWAY; ALTERNATIVE COMPLEMENT PATHWAY; and LECTIN COMPLEMENT PATHWAY).
Lupus Erythematosus, Systemic
A chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow.
Theileriasis
Chromatography, Affinity
Leishmania tropica
Leishmaniasis
A disease caused by any of a number of species of protozoa in the genus LEISHMANIA. There are four major clinical types of this infection: cutaneous (Old and New World) (LEISHMANIASIS, CUTANEOUS), diffuse cutaneous (LEISHMANIASIS, DIFFUSE CUTANEOUS), mucocutaneous (LEISHMANIASIS, MUCOCUTANEOUS), and visceral (LEISHMANIASIS, VISCERAL).
RNA, Messenger
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Erythrocytes
Autoantigens
Paramecium
Mice, Inbred Strains
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
Plasmodium
A genus of protozoa that comprise the malaria parasites of mammals. Four species infect humans (although occasional infections with primate malarias may occur). These are PLASMODIUM FALCIPARUM; PLASMODIUM MALARIAE; PLASMODIUM OVALE, and PLASMODIUM VIVAX. Species causing infection in vertebrates other than man include: PLASMODIUM BERGHEI; PLASMODIUM CHABAUDI; P. vinckei, and PLASMODIUM YOELII in rodents; P. brasilianum, PLASMODIUM CYNOMOLGI; and PLASMODIUM KNOWLESI in monkeys; and PLASMODIUM GALLINACEUM in chickens.
Antibody-Dependent Cell Cytotoxicity
The phenomenon of antibody-mediated target cell destruction by non-sensitized effector cells. The identity of the target cell varies, but it must possess surface IMMUNOGLOBULIN G whose Fc portion is intact. The effector cell is a "killer" cell possessing Fc receptors. It may be a lymphocyte lacking conventional B- or T-cell markers, or a monocyte, macrophage, or polynuclear leukocyte, depending on the identity of the target cell. The reaction is complement-independent.
Plasmodium falciparum
Lymphocyte Activation
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Cattle Diseases
DNA
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Single-Domain Antibodies
Trypanocidal Agents
Antigens, CD
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
Linear peptide specificity of bovine antibody responses to p67 of Theileria parva and sequence diversity of sporozoite-neutralizing epitopes: implications for a vaccine. (1/3338)
A stage-specific surface antigen of Theileria parva, p67, is the basis for the development of an anti-sporozoite vaccine for the control of East Coast fever (ECF) in cattle. By Pepscan analysis with a series of overlapping synthetic p67 peptides, the antigen was shown to contain five distinct linear peptide sequences recognized by sporozoite-neutralizing murine monoclonal antibodies. Three epitopes were located between amino acid positions 105 to 229 and two were located between positions 617 to 639 on p67. Bovine antibodies to a synthetic peptide containing one of these epitopes neutralized sporozoites, validating this approach for defining immune responses that are likely to contribute to immunity. Comparison of the peptide specificity of antibodies from cattle inoculated with recombinant p67 that were immune or susceptible to ECF did not reveal statistically significant differences between the two groups. In general, antipeptide antibody levels in the susceptible animals were lower than in the immune group and neither group developed high responses to all sporozoite-neutralizing epitopes. The bovine antibody response to recombinant p67 was restricted to the N- and C-terminal regions of p67, and there was no activity against the central portion between positions 313 and 583. So far, p67 sequence polymorphisms have been identified only in buffalo-derived T. parva parasites, but the consequence of these for vaccine development remains to be defined. The data indicate that optimizations of the current vaccination protocol against ECF should include boosting of relevant antibody responses to neutralizing epitopes on p67. (+info)Preparation of antibodies directed to the Babesia ovata- or Theileria sergenti-parasitized erythrocytes. (2/3338)
To investigate the surface antigens of the bovine red blood cells (RBCs) parasitized by Babesia ovata or Theileria sergenti, attempts were made to produce monoclonal antibodies (mAbs) with BALB/c mice. Comparable numbers of hybridomas producing anti-piroplasm mAbs, as well as anti-bovine RBC mAbs, were obtained from the mice immunized with B. ovata- or T. sergenti-PRBCs. However, mAbs directed to the surface of parasitized RBCs (PRBCs) were obtained only from the mice immunized with B. ovata-PRBCs, but not from those immunized with T. sergenti-PRBCs. When serum samples from the immunized mice and the infected cattle were examined, antibodies recognizing B. ovata-PRBC surface were detected in the sera against B. ovata, but analogous antibodies were undetectable in the sera against T. sergenti, despite that the sera showed substantial antibody titers to T. sergenti piroplasms. The results suggest that significant antigenic modifications occur on the surface of B. ovata-PRBCs, but not on the surface of T. sergenti-PRBCs. (+info)Immunoglobulin subclass distribution and diagnostic value of Leishmania donovani antigen-specific immunoglobulin G3 in Indian kala-azar patients. (3/3338)
Visceral leishmaniasis, or kala-azar, a fatal tropical disease, remains problematic, as early diagnosis is difficult and treatment often results in drug resistance and relapse. We have developed a sensitive enzyme-linked immunosorbent assay (ELISA), using leishmanial membrane antigenic extracts (LAg) to detect specific antibody responses in 25 untreated Indian visceral leishmaniasis patients. To investigate the pathogenetic significance of isotype markers in kala-azar, relative levels of specific immunoglobulin G (IgG), IgM, IgA, IgE, and IgG subclasses were analyzed under clinically established diseased conditions. Since LAg showed higher sensitivity for specific IgG than lysate, the immunoglobulin isotype responses were evaluated, with LAg as antigen. Compared to 60 controls, which included patients with malaria, tuberculosis, leprosy, and typhoid and healthy subjects, visceral leishmaniasis patients showed significantly higher IgG (100% sensitivity, 85% specificity), IgM (48% sensitivity, 100% specificity), and IgE (44% sensitivity, 98.3% specificity) responses. Low levels of IgA in visceral leishmaniasis patients contrasted with a 13-fold-higher reactivity in sera from patients with leprosy. Among IgG subclasses, IgG1, -3, and -4 responses were significantly higher in visceral leishmaniasis patients than in the controls. IgG2 response, however, was significantly higher (twofold) in leprosy than even visceral leishmaniasis patients. The rank orders for sensitivity (IgG = IgG1 = IgG3 = IgG4 > IgG2 > IgM > IgE > IgA) and specificity (IgM = IgG3 > IgE > IgG4 > IgG2 > IgG > IgG1 > IgA) for LAg-specific antibody responses suggest the potentiality of IgG3 as a diagnostic marker for visceral leishmaniasis. (+info)Enzyme-linked immunosorbent assay for IgA antibodies to Trypanosoma cruzi in congenital infection. (4/3338)
With the aim of achieving earlier diagnosis of congenital Trypanosoma cruzi infection, we assessed the usefulness of detecting specific IgA antibody by an ELISA. We evaluated 12 pregnant women chronically infected with T. cruzi, their newborn infants, and three additional neonates with parasitemia at birth. The IgA-specific antibody was detected by adapting the procedure for use of a commercial IgG ELISA, the Hemagen Chagas' Kit (Hemagen Diagnostics, Inc., Waltham, MA). Trypanosoma cruzi-specific IgA was detected in 10 (83%) of 12 mothers at delivery, in one of three parasitemic infants, and one of 12 newborns of the chronically infected women. Testing of 13 infants at six months of age revealed IgA in seven infants (54%), of whom four also had persistent T. cruzi-specific IgG. Detection of T. cruzi-specific IgA could provide a criterion for diagnosis of congenital infection in the absence of detectable parasitemia. (+info)Prevalence of intestinal parasite infections with special reference to Entamoeba histolytica on the island of Bioko (Equatorial Guinea). (5/3338)
The prevalence of intestinal parasitic infections was assessed (1993 through 1995) among two different groups of persons on the island of Bioko, Equatorial Guinea. In the first group, parasitologic examinations were performed on stool specimens from a household-based sample of 557 dwellers from the rural area of the island. In the second group, 1,633 inpatients and outpatients at the General Hospital of Malabo (the capital of the country) were studied. All age groups were represented in both groups. The average prevalence of the most common protozoan and helminthic intestinal infections in rural and urban areas, respectively, was as follows: Entamoeba histolytica/E. dispar (14.9% and 32.7%, respectively), Giardia lamblia (7.2% and 8.6%), Ascaris lumbricoides (45.8% and 31.4%), and Trichuris trichiura (25.7% and 36.4%). Seventy-nine sera from patients with amebic liver abscess (suspected by ultrasonography) were studied by an immunohemagglutination assay, with 44 (56%) showing anti-E. histolytica titers > or = 1:32. Of these 79 sera, 71 were studied by an enzyme immunoassay, 86% of which were positive with titers > or = 1:64. This study showed that parasitic infections in Equatorial Guinea represent a major health problem. (+info)Immunization of mice with DNA-based Pfs25 elicits potent malaria transmission-blocking antibodies. (6/3338)
Immunological intervention, in addition to vector control and malaria chemotherapy, will be needed to stop the resurgence of malaria, a disease with a devastating impact on the health of 300 to 500 million people annually. We have pursued a vaccination strategy, based on DNA immunization in mice with genes encoding two antigens present on the sexual stages of Plasmodium falciparum, Pfs25 and Pfg27, to induce biologically important antibodies that can block development of the parasite in the Anopheles mosquito and thus transmission of the disease. DNA encoding Pfs25 when administered by the intramuscular route, either alone or with DNA encoding Pfg27, had the most potent transmission-blocking effects, resulting in up to a 97% decrease in oocyst numbers in mosquito midguts and a 75% decrease in rate of infection. Immunization with DNA encoding a Pfg27-Pfs25 fusion protein was less effective and DNA encoding Pfg27 elicited antibodies in sera that had only modest effects on the infectivity of the parasite. These results show for the first time that DNA vaccination can result in potent transmission-blocking antibodies in mice and suggest that the Pfs25 gene should be included as part of a multicomponent DNA vaccine. (+info)Antibodies reactive with the N-terminal domain of Plasmodium falciparum serine repeat antigen inhibit cell proliferation by agglutinating merozoites and schizonts. (7/3338)
The serine repeat antigen (SERA) is a vaccine candidate antigen of Plasmodium falciparum. Immunization of mice with Escherichia coli-produced recombinant protein of the SERA N-terminal domain (SE47') induced an antiserum that was inhibitory to parasite growth in vitro. Affinity-purified mouse antibodies specific to the recombinant protein inhibited parasite growth between the schizont and ring stages but not between the ring and schizont stages. When Percoll-purified schizonts were cultured with the affinity-purified SE47'-specific antibodies, schizonts and merozoites were agglutinated. Indirect-immunofluorescence assays with unfixed parasite cells showed that SE47'-specific immunoglobulin G (IgG) bound to SERA molecules on rupturing schizonts and merozoites but the IgG did not react with the schizont-infected erythrocytes (RBC). Furthermore, double-fluorescence staining against SE47'-specific IgG and anti-human RBC membrane IgG showed that the RBC membrane disappeared from SE47'-specific-IgG-bound schizonts after cultivation. These observations suggest that the SE47'-specific antibodies inhibit parasite growth by cross-linking SERA molecules that are associated with merozoites in rupturing schizonts with partly broken RBC and parasitophorous vacuole membranes, blocking merozoite release. (+info)Value of Western blotting in the clinical follow-up of canine leishmaniasis. (8/3338)
Specific serum antibody levels in Leishmania infantum-infected dogs treated with a combination of glucantime and allopurinol were estimated by indirect immunofluorescence and Western blotting. The sensitivity of Western blot was greater than that obtained with immunofluorescence titration. In general, both diagnostic methods concurred with the post-treatment clinical status of the animals. Clinical improvement of successfully treated dogs was related to lower immunofluorescence titers and simpler and/or less reactive immunodetection patterns in Western blotting. The recognition, by infected dogs, of certain low molecular weight antigens, particularly one of approximately 26 kDa, was restricted to pretreatment samples and a single animal in relapse thus apparently constituting an active infection marker. (+info)
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Malabsorption
IgA Anti-transglutaminase antibodies or IgA Anti-endomysial antibodies for Coeliac disease (gluten sensitive enteropathy). ... Microscopy is particularly useful in diarrhoea, may show protozoa like Giardia, ova, cyst and other infective agents. Fecal fat ...
Philasterides dicentrarchi
... against the protozoan parasite Philasterides dicentrarchi: Effects on antibody production and protection". Journal of Fish ... Philasterides dicentrarchi is a marine protozoan ciliate that was first identified in 1995 after being isolated from infected ... formalin and adjuvant concentration on antibody response and protection against the pathogen". Aquaculture. 278 (1-4): 22-26. ...
Malaria vaccine
Antibodies that interfere with the binding, by either targeting the key region of the DARC or the DBP will prevent the ... By their very nature, protozoa are more complex organisms than bacteria and viruses, with more complicated structures and life ... Antibodies against the Schizont Egress Antigen-1 (PfSEA-1) were found to disable the parasite ability to rupture from the ... Antibodies are part of the specific immune response. They exert their effect by activating the complement cascade, stimulating ...
Crithidia luciliae
... a type of anti-nuclear antibody. Anti-nuclear antibodies are a common feature in SLE, and anti-dsDNA antibodies are highly ... As a parasitic protozoan, C. luciliae lacks the ability to biosynthetically produce purine bases and therefore needs to salvage ... C. luciliae is a eukaryotic single-cell protozoan. The family Trypanosomatidae belongs to the order Kinetoplastida and is ... The kinetoplast found in C. luciliae allows them to be used for the detection of anti-dsDNA antibodies, ...
Sarcocystis neurona
The protozoan Sarcocystis neurona is a protozoan of single celled character and belongs to the family, Sarcocystidae, a group ... Antibody detection in cerebrospinal fluid (CSF) offers more insight, but tends to be cofounded by flood contamination during ... Transmission of the protozoan from the infected horse to other animals is not possible based on the schizonts and daughter ... The protozoan, S. neurona, is a member of the genus Sarcocystis, and is most commonly associated with equine protozoal ...
Owais Mohammad
182550). The monoclonal antibody bearing liposomes with encapsulated chq were found to be highly effective in the treatment of ... protozoan (malaria, leishmaniasis) and fungal (candidasis and cryoptococcosis) origin. In general, specialized groups of ... It was further demonstrated that the entrapment of anti-malarial drugs like chloroquine (chq), in the antibody-coated liposomes ... Encouraged by these results, the liposomes were coated with F(ab')2 fragments of a monoclonal antibody which specifically ...
Toxoplasma gondii
In a meta-analysis of 23 studies that met inclusion criteria, the seroprevalence of antibodies to T. gondii in people with ... In 1908, while working at the Pasteur Institute in Tunis, Charles Nicolle and Louis Manceaux discovered a protozoan organism in ... Minks, being semiaquatic, are also susceptible to infection and being antibody-positive toward Toxoplasma gondii. Minks can ... Toxoplasma gondii (/ˈtɒksoʊplæzmə ˈɡɒndiaɪ/) is an obligate intracellular parasitic protozoan eukaryote (specifically an ...
Z-RNA
Formation of Z-RNA in living cells was suggested by experiments using anti-Z-RNA antibodies to stain fixed protozoan cells ...
Confocal microscopy
β-tubulin in Tetrahymena (a ciliated protozoan). Partial surface profile of a 1-Euro coin, measured with a Nipkow disk confocal ... Green signal from anti-tubulin antibody conjugated with Alexa Fluor 488) and nuclei (blue signal from DNA stained with DAPI) in ...
Babesia microti
... while the malaria protozoans are spread via mosquito. Finally, under the microscope, the merozoite form of the B. microti ... the FDA approved an antibody-based screening test for blood and organ donors. An important difference from malaria is that B. ...
Influenza A virus
... an antibody that targets the hemagglutinin protein, was discovered in 2011. FI6 is the only known antibody effective against ... protozoa and fungi that are forever changing and adapting themselves to the human host and the defenses that humans create. ... The H5N2 antibody titers of paired sera of 13 subjects increased fourfold or more. H5N8 In February 2021, Russia reported the ... Furthermore, they are also the antigen proteins to which a host's antibodies can bind and trigger an immune response. Influenza ...
Ticks of domestic animals
... the ticks act as long term reservoirs of the protozoans. In addition, some species of protozoans (within the Theileria and ... Vaccinated cattle develop antibodies circulating in their blood. When the Rh. microplus female ticks engorge with blood, the ... These pathogenic protozoa can be detected circulating in populations of the cattle hosts and tick vectors with only low levels ... Babesia bovis protozoa are transmitted by R. microplus and cause babesiosis or redwater fever in cattle throughout the tropics ...
Lucy Graves Taliaferro
... which the Taliaferros found to initiate formation of not only antibodies that had lytic properties, but also antibodies that ... She specialized in tropical parasitology, specifically trypanosomes, a group of unicellular parasitic protozoa found in ... Transfer of antibody-forming capacity in splenic materials. Science, 119:585-86. 1955 With W. H. Taliaferro. Reactions of the ... The localization of X-ray injury to the initial phases of antibody response. J. Infect. Dis., 91:105-24. 1954 With W. H. ...
Outline of immunology
Antibodies Kinds of antibodies Monoclonal antibodies Polyclonal antibodies Autoantibody Microantibody Neutralizing antibody ... Pathogens Pathogenic bacteria Viruses Fungi Protozoa Parasites Tumors Allergens Self-proteins Autoimmunity Alloimmunity Cross- ... Antibody-dependent cell-mediated cytotoxicity (ADCC) Foreign Pernicious anemia Hemolytic disease of the newborn Autoimmune ... A LILRA1 LILRA2 LILRA3 LILRA4 LILRA5 LILRA6 LILR subfamily B LILRB1 LILRB2 LILRB3 LILRB4 LILRB5 Eicosanoid receptors Antibodies ...
Occupational hazards of human nail dust
It has been suggested that absorption of trichophyton fungal antigens can give rise to immunoglobulin E (IgE) antibody ... Possible ocular hazards result from exposure to foreign bodies, allergens, bacteria, viruses, fungi and protozoa that can be ... Studies conducted in England found that the prevalence of trichophyton rubrum antibodies in podiatrists ranged from 14%-31%. ... of the population has allergic antibodies to fungal antigens, and half of them, that is 5% of the population, would be ...
Pasteur Institute
Alphonse Laveran got the 1907 Nobel Prize for his research on the role of protozoans as disease agents (notably, his discovery ... demonstrated that the serum of an animal vaccinated against the disease included the antibodies needed to defeat it. The anti- ... as the endotoxins alone are sufficient to trigger the production of antibodies. Regarding curative medicine, it was in 1911 ... especially the implication of antibodies and the mechanisms of action of the complement; Charles Nicolle received the Nobel ...
Frederick Parker Gay
He continued to research antibodies and antigens. In 1918 he published his book on typhoid fever, and in 1921 he became Head of ... He contributed to Agents of Disease and Host Resistance(1935) concerned with bacteria, fungi, protozoa, rickettsiae and viruses ... 1912: (with G.Y. Rusk) Studies in the Locus of Antibody Formation, Fifteenth International Congress on Hygiene and Demography, ...
Protozoan infection
... blood tests detect antibody to parasite for visceral leishmaniasis. The second pathogen from this phylum is Trypanosoma cruzi. ... Protozoan infections are parasitic diseases caused by organisms formerly classified in the Kingdom Protozoa. They are usually ... Some protozoa are photoautotrophic protists. These protists include strict aerobes, and use photosystems I and II in order to ... Protozoa are chemoorganotrophic protists and have three different ways of acquiring nutrients. The first method of acquiring ...
Discovery of disease-causing pathogens
Many viruses, bacteria, protozoa, fungi, helminthes and prions are identified as a confirmed or potential pathogen. In the ... An additional study found that many IBS patients from whom Blastocystis could not be identified showed a strong antibody ... The most prevalent protozoa in these studies are considered emerging infectious diseases by some researchers, because a ... Giardiasis is a disease caused by infection with the protozoan Giardia lamblia. Infection with Giardia can produce diarrhea, ...
Amyloodinium ocellatum
The protozoan reproduces asexually, the first division is longitudinal while the succeeding ones are approximately regular and ... The ELISA assay might be useful for monitoring levels of protection in susceptible populations, as elevated antibody titres ... The protozoan pathogenicity is associated with the trophont attachment to host tissues; trophonts constantly twist and turn, ... In this phase, the protozoan is round and encapsulated in a cellulose wall, which becomes thicker and confers upon it an ...
Immunoglobulin E
June 2010). "Antibodies specific for a segment of human membrane IgE deplete IgE-producing B cells in humanized mice". The ... IgE is utilized during immune defense against certain protozoan parasites such as Plasmodium falciparum. IgE may have evolved ... In the second approach, antibodies specific for a domain of 52 amino acid residues, referred to as CεmX or M1' (M1 prime), ... Immunoglobulin E (IgE) is a type of antibody (or immunoglobulin (Ig) "isotype") that has been found only in mammals. IgE is ...
List of MeSH codes (D12.776.124)
... antibodies, phospho-specific MeSH D12.776.124.486.485.114.252 - antibodies, protozoan MeSH D12.776.124.486.485.114.254 - ... antibodies, phospho-specific MeSH D12.776.124.790.651.114.252 - antibodies, protozoan MeSH D12.776.124.790.651.114.254 - ... antibodies MeSH D12.776.124.486.485.114.071 - antibodies, anti-idiotypic MeSH D12.776.124.486.485.114.089 - antibodies, ... antibodies, bispecific MeSH D12.776.124.486.485.114.143 - antibodies, blocking MeSH D12.776.124.486.485.114.167 - antibodies, ...
Toxoplasmosis
The IgM antibodies appear sooner after infection than the IgG antibodies and disappear faster than IgG antibodies after ... Splendore reported the protozoan in a rabbit, while Nicolle and Manceaux identified it in a North African rodent, the gundi ( ... IgG antibodies usually appear within a week or two of infection, peak within one to two months, then decline at various rates. ... Toxoplasma IgG antibodies generally persist for life, and therefore may be present in the bloodstream as a result of either ...
Blastocystosis
These antibodies, known as immunoglobulin A (IgA), make up the immune defense system of human by preventing the growth of ... Khalifa AM, El Temsahy MM, Abou El Naga IF (2001). "Effect of ozone on the viability of some protozoa in drinking water". ... Fecal antibody testing: A 2003 study at Ain Shams University in Egypt indicated that patients symptomatically infected could be ... By breaking up the antibodies, it allows the persistence of Blastocystis in the human gut. Another more recent study has also ...
Amoebiasis
... protozoa and from cysts of free-living protozoa as discussed above. Recovery procedures are not very accurate; cysts are easily ... The levels of antibody are much higher in individuals with liver abscesses. Serology only becomes positive about two weeks ... It is important to distinguish the E. histolytica cyst from the cysts of nonpathogenic intestinal protozoa such as Entamoeba ... Since antibodies may persist for years after clinical cure, a positive serological result may not necessarily indicate an ...
Babesia
About 40 cases of human babesiosis, caused by intraerythrocytic protozoans (protozoa inside red blood cells) of the genus ... To supplement a blood smear, diagnoses should be made with an indirect fluorescent antibody (IFA) test. IFA testing has a much ... In different environments, individual protozoa may develop mutations which, when they increase the protozoa's fitness, allow ... As a protozoan parasite, the most effective way to identify Babesia infection is through blood sample testing. Babesia species ...
Hepatocystis
The protozoa are transmitted by the bite of the insect vector. The sporozoites migrate to the liver where they typically form ... Dracopoli NC, Turner TR, Else JG, Jolly CJ, Anthony R, Gallo RC, Saxinger WC (1986). "STLV-I antibodies in feral populations of ... Mature gametocytes are larger than a normal erythrocyte stain poorly compared to other protozoa. In both male and female ... Bray RS (1984). "Some parasitic protozoa from the Gambia". J. Euk. Microbiol. 31 (4): 577-8. doi:10.1111/j.1550-7408.1984. ...
Premunition
Antibody action contributes to premunition. However, premunition is probably much more complex than simple antibody and antigen ... Premunity is progressive development of immunity in individuals exposed to an infective agent, mainly belonging to protozoa and ... In the case of malaria, the sporozoite and merozoite stages of Plasmodium elicit the antibody response which leads to ... However, Plasmodium can change its surface antigens, so the development of an antibody repertoire that can recognize multiple ...
Lactobacillus vaccine
Antibodies in mucosal secretions represent the first line of immune defense of the mucosae. They are capable to bind to ... Specific immunofluorescence was observed on those protozoa which had been treated with anti-lactobacillus serum and anti- ... April 1987). "IgA antibody-producing cells in peripheral blood after antigen ingestion: evidence for a common mucosal immune ... The production of specific antibodies both in serum and in the vaginal secretion has been demonstrated. As a result of the ...
Eimeria maxima
... is a protozoan that causes coccidiosis in poultry. It is located in the middle part of the intestine, on either ... "Passive immunization of chickens against Eimeria maxima infection with a monoclonal antibody developed against a gametocyte ...
Lyme disease
The OspC antibodies kill any of the bacteria that have not been killed by the OspA antibodies. Canine Recombinant Lyme, ... IgM and IgG antibody levels may be elevated for years even after successful treatment with antibiotics.[23] As antibody levels ... because the IgM antibodies may remain after the initial infection, and IgG antibodies may remain for years.[120] ... The immune system takes some time to produce antibodies in quantity. After Lyme infection onset, antibodies of types IgM and ...
Artificial induction of immunity
Engineers of small-scale humanised antibody production. Prices on application.. *^ Immunisation article in Ganfyd, the online ... because the antibodies which are transferred have a lifespan of only about 3-6 months.[18] Every placental mammal (which ... known as antibodies or immunoglobulins. This was first performed (and is still sometimes performed) by taking blood from a ... In the future it might be possible to artificially design antibodies to fit specific antigens, then produce them in large ...
Index of HIV/AIDS-related articles
... antibodies - antibody-dependent cell-mediated cytotoxicity (ADCC) - antibody-mediated immunity - antifungal medication - ... protozoa - provirus - pruritus - pseudo-Cushing's syndrome - pseudovirion - PUBMED - pulmonary - purified protein derivative ( ... neutralizing antibody - neutralizing domain - neutropenia - neutrophil - New Drug Application (NDA) - New York Cares - NIAID - ... functional antibody - fungus - fusin - fusion inhibitor - fusion mechanism - fusion peptide ...
Pasteur Institute
Alphonse Laveran got the 1907 Nobel Prize for his research on the role of protozoans as disease agents (notably, his discovery ... Jules Bordet received the Nobel prize in 1919 for his discoveries on immunity, especially the implication of antibodies and the ... demonstrated that the serum of an animal vaccinated against the disease included the antibodies needed to defeat it. The anti- ... as the endotoxins alone are sufficient to trigger the production of antibodies. ...
Innate immune system
The complement system is a biochemical cascade of the immune system that helps, or "complements", the ability of antibodies to ... Intracellular protozoa. *Plasmodium malariae. *Leishmania donovani. no. no. no Extracellular protozoa. *Entamoeba histolytica ... Activation of the complement cascade to identify bacteria, activate cells, and promote clearance of antibody complexes or dead ... Invertebrates do not possess lymphocytes or an antibody-based humoral immune system, and it is likely that a multicomponent, ...
Glossary of biology
The part of an enzyme or antibody at which substrate molecules bind and undergo a chemical reaction.. active transport. ... The study of microscopic organisms, such as bacteria, viruses, archaea, fungi and protozoa. This discipline includes ... They are a major component of the group of immune defense molecules collectively called antibodies.. infection. The invasion of ... Rajewsky, Klaus (1996). "Clonal selection and learning in the antibody system". Nature. 381 (6585): 751-758. doi:10.1038/ ...
Immunoglobulin E
"Antibody structure". Archived from the original on September 6, 2008.. *^ Erb KJ (2007). "Helminths, allergic disorders and IgE ... IgE is utilized during immune defense against certain protozoan parasites such as Plasmodium falciparum.[6] ... Chang TW, Wu PC, Hsu CL, Hung AF (2007). Anti-IgE antibodies for the treatment of IgE-mediated allergic diseases. Adv. Immunol ... Immunoglobulin E (IgE) is a type of antibody (or immunoglobulin (Ig) "isotype") that has only been found in mammals. IgE is ...
Confocal microscopy
Green signal from anti-tubulin antibody conjugated with Alexa Fluor 488) and nuclei (blue signal from DNA stained with DAPI) in ...
Immunologic adjuvant
These observations suggest that although TLR activation can lead to increases in antibody responses, TLR activation is not ... an immune signal that promotes antibody production. Alum adheres to the cell's plasma membrane and rearranges certain lipids ... "Adjuvant-enhanced antibody responses in the absence of toll-like receptor signaling". Science. 314 (5807): 1936-8. Bibcode ... "Vaccines with the MF59 Adjuvant Do Not Stimulate Antibody Responses against Squalene". Clin. Vaccine Immunol. 13 (9): 1010- ...
Mitosis
Raikov, IB (1994). "The diversity of forms of mitosis in protozoa: A comparative review". European Journal of Protistology. 30 ... Mitotic cells can be visualized microscopically by staining them with fluorescent antibodies and dyes. ...
Hantavirus vaccine
Antibody responses in humans to an inactivated hantavirus vaccine (Hantavax). Vaccine. 1999;17:2569-75. ...
Protist
Protozoa." (First class. Primordial animals. Protozoa.) [Note: each column of each page of this journal is numbered; there are ... "Isolation of Balamuthia mandrillaris-specific antibody fragments from a bacteriophage antibody display library". Experimental ... Cox, F.E.G. (1991). "Systematics of parasitic protozoa". In: Kreier, J.P. & J. R. Baker (ed.). Parasitic Protozoa, 2nd ed., vol ... Protozoa. Thiere, in welchen die verschiedenen Systeme der Organe nicht scharf ausgeschieden sind, und deren unregelmässige ...
Giardia lamblia
B) is the cyst wall selectively imaged through use of fluorescent-labelled (TRITC) antibody that is cyst wall specific.. (C) is ... Kaneda Y, Tanaka T, Saw T (1990). "Effects of berberine, a plant alkaloid, on the growth of anaerobic protozoa in axenic ... People with recurring Giardia infections, particularly those with a lack of the Immunoglobulin A antibody, may develop chronic ... është një parazit flagellated protozoan që kolonizon dhe riprodhohet në intestine të vogla, që shkakton giardiasis. Paraziti i ...
Babesiosis
Serologic testing for antibodies against Babesia (both IgG and IgM) can detect low-level infection in cases with a high ... Babesia species are in the phylum Apicomplexa, which also has the protozoan parasites that cause malaria, toxoplasmosis, and ... April 1994). "Diagnosis of babesiosis: evaluation of a serologic test for the detection of Babesia microti antibody". J. Infect ... Since detectable antibody responses require about a week after infection to develop, serologic testing may be falsely negative ...
ಟೆಂಪ್ಲೇಟು:Central nervous system navs - ವಿಕಿಪೀಡಿಯ
Template:Protozoan infection navs(edit talk links history). *Template:Psych navs(edit talk links history) ({{Psychology navs ...
Infection
For example, the use of antibodies made artificially fluorescent (fluorescently labeled antibodies) can be directed to bind to ... Several human activities have led to the emergence of zoonotic human pathogens, including viruses, bacteria, protozoa, and ... These tests are based upon the ability of an antibody to bind specifically to an antigen. The antigen, usually a protein or ... Immunoassay B on the other hand may detect or measure antibodies produced by an organism's immune system that are made to ...
List of Nobel Prize winners in Physiology or Medicine - Simple English Wikipedia, the free encyclopedia
1907 - Alphonse Laveran, France, for his work on the way protozoa can cause disease[7] ... 1987 - Susumu Tonegawa, Japan, for his discovery how the genes make different antibodies[78] ... for theories about the development and control of the immune system and the discovery of monoclonal antibodies are made[75] ... for finding out the chemical structure of antibodies[63] ...
ಪ್ರತಿರಕ್ಷಣೆ(ಇಮ್ಯೂನೈಸೇಷನ್) - ವಿಕಿಪೀಡಿಯ
ಪ್ರತಿರಕ್ಷಣೆ ಅಥವಾ ಇಮ್ಯೂನೈಸೇಷನ್ ಎನ್ನುವುದು ಒಂದು ಪ್ರಕ್ರಿಯೆಯಾಗಿದ್ದು, ಅದರಲ್ಲಿ ವ್ಯಕ್ತಿಗಳ ಪ್ರತಿರೋಧಕ ವ್ಯವಸ್ಥೆಯು ರೋಗಕಾರಕ ಪದಾರ್ಥದ ವಿರುದ್ದ (ಇಮ್ಯುನೋಜೆನ್ ಎಂದು ಕರೆಯಲಾಗುತ್ತದೆ) ಬಲಗೊಳ್ಳುತ್ತದೆ.. ಪ್ರತಿರೋಧಕ ವ್ಯವಸ್ಥೆಯು ದೇಹಕ್ಕೆ ಬಾಹ್ಯವಾಗಿರುವ ಕಣಗಳಿಗೆ (ಸ್ವಂತದಲ್ಲದ ) ಒಡ್ಡಲ್ಪಟ್ಟಾಗ, ಅದು ಪ್ರತಿರೋಧಕ ಪ್ರತಿಕ್ರಿಯೆಯೊಂದನ್ನು ಸಂಯೋಜಿಸುತ್ತದೆ, ಆದರೆ ಅದು ಅನಂತರದ ಸಂಘರ್ಷಗಳಿಗೆ (ಪ್ರತಿರಕ್ಷಾ ಸ್ಮರಣೆಯ ಮೂಲಕ) ತ್ವರಿತವಾಗಿ ...
ಮಲೇರಿಯಾ - ವಿಕಿಪೀಡಿಯ
1986). "Antibodies to the glutamate dehydrogenase of Plasmodium falciparum". Parasitology. 92, : 313-24. doi:10.1017/ ... Infectious diseases - Parasitic disease: protozoan infection: Excavata (A06-A07, B55-B57, 007, 085-086) ... an in vitro assay of protective antibodies". J Immunol. 132 (2): 909-13. PMID 6317752.. CS1 maint: multiple names: authors list ...
Magic bullet (medicine)
Research on antibody[edit]. In the early 1890s, Paul Ehrlich started to work with Emil Behring, professor of medicine at the ... a protozoan parasite that causes sleeping sickness. In 1904 they successfully prepared a red arsenic dye they called Trypan Red ... Ehrlich was also nominated for that year.[5]) From Behring's work, Ehrlich understood that antibodies produced in the blood ... He speculated that these antibodies act as bullets fired from a gun to target specific microbes. But after further research, he ...
Chagas disease
Infectious diseases - Parasitic disease: protozoan infection: Excavata (A06-A07, B55-B57, 007, 085-086) ... With the exception of blood derivatives (such as fractionated antibodies), all blood components are infective. The parasite ... Burkholder JE, Allison TC, Kelly VP (April 1980). "Trypanosoma cruzi (Chagas) (Protozoa: Kinetoplastida) in invertebrate, ... harbored a flagellate protozoan, a new species of the genus Trypanosoma, and was able to demonstrate experimentally that it ...
Pathogen
Examples of eukaryotic pathogens capable of sex include the protozoan parasites Plasmodium falciparum, Toxoplasma gondii, ... Treating the symptoms of a viral infection gives the host immune system time to develop antibodies against the viral pathogen ... protozoan, prion, viroid, or fungus.[3][4][5] Small animals, such as certain kinds of worms and insect larvae, can also produce ... Some eukaryotic organisms, including a number of protozoa and helminths, are human parasites. ...
Pfizer
Flagyl (metronidazole) is a nitroimidazole antibiotic medication used particularly for anaerobic bacteria and protozoa. It is ... PD-L1 monoclonal antibody, CS1001.[58] In October, Pfizer announced it had acquired Arixa Pharmaceuticals.[59] ...
T helper cell
Stimulates B-cells into proliferation, to induce B-cell antibody class switching, and to increase neutralizing antibody ... In addition, IFN-γ can activate iNOS to produce NOx free radicals to directly kill intracellular bacteria and protozoa. Th1 ... Antibodies do not play a direct role in this allergy type. T cells play an important role in this hypersensitivity, as they ... They are essential in B cell antibody class switching, in the activation and growth of cytotoxic T cells, and in maximizing ...
Ang Sakit na Chagas, ang malayang ensiklopedya
... ay isang tropikal na sakit na dulot ng parasite o parasito na dulot ng protozoan Trypanosoma cruzi.[1] Ito ay kadalasang ... Ang pagkakatukoy ng matagalang sakit ay sa pamamagitan ng pagkakakita ng mga antibody para sa T. cruzi sa dugo.[3] ...
Galactose
Zanetti, Maurizio; Capra, Donald J. (2003-09-02). The Antibodies. CRC Press. p. 78. ISBN 9780203216514.. ... Galactofuranose occurs in bacteria, fungi and protozoa,[7] and is recognized by a putative chordate immune lectin intelectin ...
Beekeeping
Beekeepers have high levels of antibodies (mainly IgG) reacting to the major antigen of bee venom, phospholipase A2 (PLA).[30] ... The common agents of disease that affect adult honey bees include fungi, bacteria, protozoa, viruses, parasites, and poisons. ... HELD, W.; STUCKI, M.; HEUSSER, C.; BLASER, K. (February 1989). "Production of Human Antibodies to Bee Venom Phospholipase A2 in ... Antibodies correlate with the frequency of bee stings. The entry of venom into the body from bee-stings may also be hindered ...
Cell nucleus
Most acantharean species of protozoa[69] and some fungi in mycorrhizae[70] have naturally multinucleated cells. Other examples ... Antibodies to certain types of chromatin organization, in particular, nucleosomes, have been associated with a number of ... Barned S, Goodman AD, Mattson DH (February 1995). "Frequency of anti-nuclear antibodies in multiple sclerosis". Neurology. 45 ( ... "The relation of immunoglobulin class, pattern of anti-nuclear antibody, and complement-fixing antibodies to DNA in sera from ...
Periodontal pathology
High correlation with protozoa Entamoeba gingivalis and Trichomonas tenax was then established. Progress in microscopy in the ... Genco RJ, Mashimo PA, Krygier G, Ellison SA (May 1974). "Antibody-mediated effects on the periodontium". J. Periodontol. 45 (5 ... The presence of complement and antigen-antibody complexes is evident throughout the connective and epithelial tissue. It is in ...
defense response to protozoan Antibodies | Invitrogen
...
Antibodies for proteins involved in defense response to protozoan pathways, according to their Panther/Gene Ontology ... Custom Antibody Service. Searching for an antibody we dont offer? We make custom antibodies for specific targets, species and ... Antibodies for proteins involved in defense response to protozoan pathways; according to their Panther/Gene Ontology ... If an Invitrogen™ antibody doesnt perform as described on our website or datasheet,well replace the product at no cost to you ...
anti-Toxoplasma Gondii (Tachyzoites) antibody</span...
Protozoa Host: Goat Clone: Polyclonal 1 PubMed reference , Order Toxoplasma Gondii (Tachyzoites) antibody (ABIN2476906). ... Protozoa. Host Goat. Clonality Polyclonal Application Immunofluorescence (IF). Options Bulk discount 1 reference available ... "Development of a multiplex microsphere immunoassay for the quantitation of salivary antibody responses to selected waterborne ...
Antibodies, protozoan | definition of Antibodies, protozoan by Medical dictionary
What is Antibodies, protozoan? Meaning of Antibodies, protozoan medical term. What does Antibodies, protozoan mean? ... protozoan in the Medical Dictionary? Antibodies, protozoan explanation free. ... Protozoa. (redirected from Antibodies, protozoan). Also found in: Dictionary, Thesaurus, Encyclopedia. Protozoa. [pro″to-zo´ah ... Antibodies, protozoan , definition of Antibodies, protozoan by Medical dictionary https://medical-dictionary.thefreedictionary. ...
Recent Articles | Protozoans, Disease/Medicine And Immunology | The Scientist Magazine®| Page 5
Recent Articles | Protozoans, Disease/Medicine And Neuroscience | The Scientist Magazine®
DNA-Delivered Antibodies Fight Off Lethal Bacterial Infection. By Catherine Offord , October 3, 2017 ... tags: protozoans x disease/medicine x neuroscience x cell & molecular biology x ... Mice receiving the treatment produced their own monoclonal antibodies and survived infection with the life-threatening pathogen ...
GENTAUR antibody-antibodies.com The Marketplace for Antibodies : Morphogenetic and molecular analyses of cyst wall components...
Morphogenetic and molecular analyses of cyst wall components in the ciliated protozoan Colpoda cucullus Nag-1. ... Find the right antibody for your research needs. ... antibody-antibodies.com is the marketplace for research ... Antibodies Cells Cloning Elisa Kits microRNA Analysis Multiplex Cytokine Assays PCR Peptides Proteins Transfection Viral ... Morphogenetic and molecular analyses of cyst wall components in the ciliated protozoan Colpoda cucullus Nag-1. The cyst wall of ...
Type 1 interferon (IFNalpha/beta) and type 2 nitric oxide synthase regulate the innate immune response to a protozoan parasite
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Results of search for 'su:{Antibodies, Protozoan.}'
Faculty Research Topics | Department of Microbiology & Immunology | Albert Einstein College of Medicine
Effect of the strain of Toxoplasma gondii on the development of toxoplasmic encephalitis in mice treated with antibody to...
Antibodies, Protozoan/blood. *Brain/parasitology*. *Brain/pathology. *Encephalitis/parasitology. *Encephalitis/pathology. * ... Treatment of mice with a monoclonal antibody to interferon-gamma (IFN-gamma) once weekly for 3 weeks beginning at 10 weeks ... Mice infected with the Beverley strain developed foci of acute inflammation in their brains after treatment with an antibody to ... Effect of the strain of Toxoplasma gondii on the development of toxoplasmic encephalitis in mice treated with antibody to ...
Seroprevalence rates of antibodies against leishmania infantum and other protozoan and rickettsial parasites in dogs - pdf...
Seroprevalence rates of antibodies against leishmania infantum and other protozoan and rickettsial parasites in dogs . Aline ... Seroprevalence rates of antibodies against leishmania infantum and other protozoan and rickettsial parasites in dogs ... Seroprevalence rates of antibodies against leishmania infantum and other protozoan and rickettsial parasites in dogs - Descarga ... Wilma Aparecida Seroprevalence rates of antibodies against Leishmania infantum and other protozoan and rickettsial parasites in ...
Studies on canine leishmaniasis control. 1. Evolution of infection of different clinical forms of canine leishmaniasis...
Prevalence of agglutinating anti-Leishmania antibodies in two multi-thousand Bengoli communities. - PubMed - NCBI
Patent US20060160245 - Methods to view and analyze the results from diffraction-based diagnostics - Google Patents
... protozoa; or antigens specific to these microbes; rheumatoid factor; antibodies, including, but not limited to IgG, IgM, IgA ... Patterned antibody-binding protein layers with bound antibodies cause patterned placement or binding of analytes thereon. ... antibodies) to these and other viruses; antigens specific to RSV or host response (antibodies) to the virus; an antigen; enzyme ... antibodies) to these and other viruses; antigens specific to RSV or host response (antibodies) to the virus; an antigen; enzyme ...
Protozoan Parasites of Rodents and Their Zoonotic Significance in Boyer-Ahmad District, Southwestern Iran
Antibodies to T. gondii were detected in the sera of 5 (9.61%) cases. Results of the molecular study showed T. gondii infection ... Moreover, 2 to 5 mL of blood was taken from each of the rodents and the sera were examined for anti-Leishmania antibodies, by ... Protozoan Parasites of Rodents and Their Zoonotic Significance in Boyer-Ahmad District, Southwestern Iran. Zeinab Seifollahi, ... The current study aimed to assess the protozoan infection of rodents in Boyer-Ahmad district, southwestern Iran. Materials and ...
The Cell - Geoffrey Cooper - Oxford University Press
Gentaur Molecular :AbD \ MOUSE ANTI CRYPTOSPORIDIUM Alexa Fluor® 488, Product Type Monoclonal Antibody, Specificity ...
Product Type Monoclonal Antibody, Specificity CRYPTOSPORIDIUM, Target Species Protozoan, Host Mouse, Format ALEXA FLUOR® 488, ... antibody-antibodies.com/anti-elisa/IgG3.html MOUSE ANTI CRYPTOSPORIDIUM Alexa Fluor® 488, Product Type Monoclonal Antibody, ... For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the ... Target Species Protozoan, Host Mouse, Format ALEXA FLUOR® 488, Isotypes IgG3, Applicat antibody storage GENTAUR recommends for ...
Seroprevalence rates of antibodies against Leishmania infantum and other protozoan and rickettsial parasites in dogs | Rev....
Full text: Available Index: LILACS (Americas) Main subject: Antibodies, Protozoan / Leishmania infantum / Dog Diseases / ... Full text: Available Index: LILACS (Americas) Main subject: Antibodies, Protozoan / Leishmania infantum / Dog Diseases / ... Seroprevalence rates of antibodies against Leishmania infantum and other protozoan and rickettsial parasites in dogs / ... Animals , Dogs , Female , Male , Antibodies, Protozoan/blood , Dog Diseases/epidemiology , Dog Diseases/parasitology , ...
WASH Benefits Kenya - Full Text View - ClinicalTrials.gov
Intestinal protozoans. Antibody response. Additional relevant MeSH terms: Diarrhea. Malnutrition. Signs and Symptoms, Digestive ... We will additionally measure Total IgG antibody titers in the blood, and we will compare groups using logged values of the ... antibody levels.. *ASQ Child Development Scores [ Time Frame: Measured 24 months after intervention ]. Interviewers will ...
WASH Benefits Bangladesh - Full Text View - ClinicalTrials.gov
Intestinal protozoans. Antibody response. Water. Sanitation. Hand washing. Breast Feeding. Complementary Feeding. ... We will additionally measure Total IgG antibody titers in the blood, and we will compare groups using logged values of the ... antibody levels.. *ASQ Child Development Scores [ Time Frame: Measured 24 months after intervention ]. Interviewers will ...
A novel protein phosphatase 2A (PP2A) is involved in the transformation of human protozoan parasite Trypanosoma cruzi |...
Abbreviations used: DMEM, Dulbeccos modified Eagles medium; DMSO, dimethyl sulphoxide; mAb, monoclonal antibody; PFGE, pulsed ... A novel protein phosphatase 2A (PP2A) is involved in the transformation of human protozoan parasite Trypanosoma cruzi. Jorge ... A novel protein phosphatase 2A (PP2A) is involved in the transformation of human protozoan parasite Trypanosoma cruzi ... A novel protein phosphatase 2A (PP2A) is involved in the transformation of human protozoan parasite Trypanosoma cruzi ...
Sex-dependent toxoplasmosis-associated differences in testosterone concentration in humans | Parasitology | Cambridge Core
Flegr J., Zitkova S., Kodym P. and Frynta D. (1996). Induction of changes in human behaviour by the parasitic protozoan ... Assessment of antitoxoplasmatic IgG antibodies with the ELISA method). Ceskoslovenska Epidemiologie 38, 355-361. ... Roberts C. W., Walker W. and Alexander J. (2001). Sex-associated hormones and immunity to protozoan parasites. Clinical ...
Antibodies Search | ALZFORUM
Acris Antibodies. monoclonal. IgG1. Rat. (liquid, supernatant, Tris/HCl, foetal calf serum, azide). ELISA, IH, IP, WB. Bovine, ... Acris Antibodies. polyclonal. Rabbit. (liquid, prrified Ig fraction, PBS, azide, affinity purified). ELISA, IB, IH, WB. Human, ... These antibodies do not cross-react with the cardiac isoform of myosin light chain 2. -. ... OC(paper on antibodies;paper on oligimerization; Charles G Glabe, University of California, Irvine)References:Lefterov, ...
Antibodies Search | ALZFORUM
Inter Research » DAO » v18
Monoclonal antibodies against sporangia and spores of Marteilla sp. (Protozoa: Ascetospora) DAO 18:211-216 , Full text in pdf ... Histopathology of Goussia clupearum (Protozoa: Apicomplexa: Coccidia) in some marine fish from Scottish waters DAO 18:195-202 ... Typing of French isolates of infectious haematopoietic necrosis virus (IHNV) with monoclonal antibodies using indirect ... Characterization of the infectious hematopoietic necrosis virus glycoprotein using neutralizing monoclonal antibodies DAO 18:29 ...
MEDLINE - Resultado p gina 1
MEDLINE - Resultado p gina 1
0 (Antibodies, Protozoan); 0 (Antigens, Protozoan); 0 (Biomarkers); 0 (Epitopes); 0 (HRP-2 antigen, Plasmodium falciparum); 0 ( ... 0 (Antigens, Protozoan); 0 (Single-Domain Antibodies); EC 4.1.2.13 (Fructose-Bisphosphate Aldolase). ... The antibodies detected the native protein in parasite lysates as a 38 kDa protein and immunofluorescence verified a parasite ... The antibodies suggested a 4-6 fold higher concentration of native PfGAPDH compared to PfLDH in immunoprecipitation and ELISA ...
Effect of different East Coast fever control strategies on disease incidence in traditionally managed Sanga cattle in Central...
Long-term preservation of blood samples for diagnosis of Trypanosoma cruzi infection.
... field research of a whole-blood preservation method was evaluated through the screening of anti-Trypanosoma cruzi antibodies in ... Antibodies, Protozoan / analysis*. Blood Preservation / methods*. Chagas Disease / diagnosis*. Evaluation Studies as Topic. ... Antibody reactivity of paired samples from preserved capillary blood (CBP) and sera from venous blood (VBS) were studied by ... field research of a whole-blood preservation method was evaluated through the screening of anti-Trypanosoma cruzi antibodies in ...
Monoclonal AntibodyParasitesTrypanosomaAntigensReactivityImmunityParasitePathogensBacteriaPlasmodiumLeishmaniaProteinsSecondary AntibodiesPrimary antibodyELISAAntigenInfectionDiagnosisIMMUNOGENProduction of antibodiesImmunofluorescenceEnzyme-linkeMalariaIntestinal protozoaMicroorganismsHumansOrganismsIsotypeDetectionInfectionsCryptosporidiumGiardiaHumoralRecombinantExtracellularCruziGENTAURToxoplasmaCystsInhibitPrevalenceTitersSalivaryDiseaseDiagnosticCiliateWaterborneSpecificSerumHumanImmuneMolecularVaccineGondii
Monoclonal Antibody2
- Treatment of mice with a monoclonal antibody to interferon-gamma (IFN-gamma) once weekly for 3 weeks beginning at 10 weeks after infection augmented the inflammatory changes in the brains of mice infected with each strain. (nih.gov)
- 1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. (avivasysbio.com)
Parasites19
- Starke Buzetti, Wilma Aparecida Seroprevalence rates of antibodies against Leishmania infantum and other protozoan and rickettsial parasites in dogs Revista Brasileira de Parasitologia Veterinária, vol. (duhnnae.com)
- The presence of antibodies against these parasites in serum samples from dogs confirmed their exposure to these parasites in this rural area . (bvsalud.org)
- The first section of this book discusses prions, viruses, protozoa, and helminth parasites both in host organisms and in food, drinking water and the environment. (indigo.ca)
- Sex-associated hormones and immunity to protozoan parasites. (ajtmh.org)
- whereas, the occurrence of eukaryotic parasites such as protozoans and microsporidians in these birds is little known. (pubmedcentralcanada.ca)
- The in vitro culture of protozoan parasites involves highly complex procedures, which are subject to many variables. (asm.org)
- There are a number of issues involved in the culture of protozoan parasites that make these procedures highly complex and subject to many variables, some of which are known and some of which are still undefined. (asm.org)
- Certainly protozoan parasites have complex life cycles. (asm.org)
- At the present time, are there any advantages in the in vitro culture of protozoan parasites, especially when revolutionary developments are going on in the field of molecular biology? (asm.org)
- HIV infection increases the burden of disease of malaria in pregnancy, in part by impairing the development of immunity.We measured total IgG and phagocytic antibodies against variant surface antigens of placental-type CS2 parasites in 187 secundigravidae (65% HIV infected).Secundigravidae may be an informative group for identification of correlates of immunity. (nih.gov)
- We measured total IgG and phagocytic antibodies against variant surface antigens of placental-type CS2 parasites in 187 secundigravidae (65% HIV infected). (nih.gov)
- Rapid card tests detecting parasite antigens and host antibodies are available for several important protozoan parasites and serve particularly well in the field setting. (els.net)
- The aim of this work was to establish a methodology for antibody delivery (antibody transfection) into viable parasites. (scirp.org)
- In electroporation was impossible to visualize the antibody inside parasites and with saponin permeabilization, antibodies were successfully introduced, but with loss of parasites viability. (scirp.org)
- Anti-cyclin antibodies were visualized in the cytoplasm from fixed transfected parasites (immunofluorescence assays). (scirp.org)
- C) Activity against parasites/protozoa Both the Bothrops brazili crude venom and its metalloproteinase (BbMP-1) are act against Plasmodium falciparum in vitro and showed low IC50 levels (0.17 and 3.2 mg/mL respectively) 71. (bartleby.com)
- The device includes a culture of live unsporulated free-living protozoan parasites. (freepatentsonline.com)
- 1. A device for testing the efficacy of a disinfectant treatment comprising (a) a culture of live unsporulated free-living protozoan parasites and (b) optionally, a receptacle for facilitating contacting of the disinfectant treatment with the culture. (freepatentsonline.com)
- 13. A method for testing the efficacy of a disinfectant treatment comprising (a) contacting a culture of live unsporulated protozoan parasites in the free-living stage with an effective amount of the disinfectant treatment, and (b) determining the percentage of protozoan parasites that have survived, wherein the culture of (a) is stored under conditions that prevent sporulation prior to the contacting. (freepatentsonline.com)
Trypanosoma6
- Feasability and suitability for field research of a whole-blood preservation method was evaluated through the screening of anti-Trypanosoma cruzi antibodies in 1209 samples under different conditions. (biomedsearch.com)
- Persistence of antibodies to Trypanosoma brucei gambiense after treatment of human trypanosomiasis in Uganda. (curehunter.com)
- This antibody recognizes Trypanosoma brucei procyclin GPEET. (acris-antibodies.com)
- Differentiation of Trypanosoma brucei, a flagellated protozoan parasite, between life cycle stages typically occurs through an asymmetric cell division process, producing two morphologically distinct daughter cells. (brookes.ac.uk)
- Chagas disease is caused by the flagellated protozoan Trypanosoma cruzi, which is mostly transmitted during a meal blood of blood‑sucking triatomine insects (kissing bugs). (bartleby.com)
- Glycolysis is essential to Trypanosoma brucei (Tb), the protozoan parasite that causes African sleeping sickness in humans and nagana in livestock, and to Trypanosoma cruzi (Tc), that causes Chagas ' Disease. (bartleby.com)
Antigens12
- Response: Involves production of antibodies and generation of specialized lymphocytes against specific antigens. (scribd.com)
- The study of reactions between antibodies and antigens. (scribd.com)
- Humoral (Antibody-Mediated) Immunity (Antibody Involves production of antibodies against foreign antigens. (scribd.com)
- Primary antibodies are the key links to detect, analyze and manipulate specific antigens. (prosci-inc.com)
- Chronic exposure to antigens may favor the production of IgG4 antibodies over other antibody types. (frontiersin.org)
- IgG4 antibodies are known to reflect repeated exposure to environmental antigens and allergens ( 22 , 23 ). (frontiersin.org)
- Using crude soluble P. falciparum antigens, an ELISA-malaria antibody test detected anti-Plasmodium antibodies. (nih.gov)
- The invention further provides antibodies, monoclonal and/or polyclonal, labeled and/or unlabeled, that react with the antigens. (freepatentsonline.com)
- The invention is also directed to a method of diagnosing Lyme disease by detecting the antigens in a biological sample taken from a host using the antibodies in conventional immunoassay formats. (freepatentsonline.com)
- The advantage of the antibodies used in the invention is that they react with the antigens from geographically diverse strains of Borrelia burgdorferi, but do not react with antigens from related Borrelia spirochetes. (freepatentsonline.com)
- The present invention relates to novel antigens associated with Borrelia burgdorferi, antibodies that are raised against the antigens and the use of the antibodies to diagnose Lyme disease and for immune capture and cultivation of microorganisms. (freepatentsonline.com)
- The present-day extensive use of enzyme immunoassays and molecular methods (e.g., polymerase chain reaction) for diagnosis and characterization of animal pathogens has its origins in the use of isotope-labeled antigens and antibodies. (springer.com)
Reactivity1
- Antibody reactivity of paired samples from preserved capillary blood (CBP) and sera from venous blood (VBS) were studied by specific techniques. (biomedsearch.com)
Immunity7
- Naturally Acquired Passive Immunity: Immunity: Antibodies pass from mother to fetus via placenta or breast feeding (colostrum). (scribd.com)
- 2. . Artificially Acquired Passive Immunity: Immunity: Preformed antibodies (antiserum) are introduced into body antiserum) by injection. (scribd.com)
- We have identified a family of genes that code for targets for opsonic antibody and protective immunity in T. pallidum subspecies pallidum using two different approaches, subtraction hybridization and differential immunologic screening of a T. pallidum genomic library. (nih.gov)
- Phagocytic antibodies to CS2(VSA) are valuable tools to study acquired immunity to malaria in the context of HIV co-infection. (nih.gov)
- Also present in the mucus are protective antibodies , which are products of specific immunity. (britannica.com)
- Immunity to Protozoa and Worms 16. (whsmith.co.uk)
- Foals were protected when antibodies providing immunity were passed on in the mares' colostrum. (horsetalk.co.nz)
Parasite6
- Our studies suggest that in T. cruzi PP2A is important for the complete transformation of trypomastigotes into amastigotes during the life cycle of this protozoan parasite. (biochemj.org)
- Previous studies have indicated that parasite-specific antibody production is important in both resistance to and pathogenesis of disease. (ajtmh.org)
- In vitro cultivation of parasitic protozoa that cause human disease is invaluable, as it provides not only information on the development of the parasite but also avenues for new approaches to the containment and/or eradication of the parasite. (asm.org)
- This polyvalent antibody immunoprecipitated S-AcP activity but did not cross-react with the surface membrane acid phosphatase, indicating that these two parasite enzymes are separate gene products. (lancs.ac.uk)
- for that reason, antibodies may be taken as a tool for studying the parasite proteins function by blocking these molecules with specific antibodies. (scirp.org)
- It was also observed that anti-cyclin-A antibodies inhibit the parasite population doubling (p (scirp.org)
Pathogens6
- Griffin, Chen, Fout, Wade, Egorov: "Development of a multiplex microsphere immunoassay for the quantitation of salivary antibody responses to selected waterborne pathogens. (antibodies-online.com)
- These potential pathogens, which include viruses , bacteria , fungi , protozoans , and worms , are quite diverse , and therefore a nonspecific defense system that diverts all types of this varied microscopic horde equally is quite useful to an organism. (britannica.com)
- pathogens, and other possible related protozoans. (umd.edu)
- The identification of protozoan pathogens is based upon direct detection of the respective causative agent in clinical specimens and/or upon detection of specific immune reactions of the host. (els.net)
- The size of protozoan pathogens varies from ∼2 μm (amastigote Leishmania spp. (els.net)
- Although numerous protocols for molecular detection of protozoan pathogens have been established in the past years, consent on the most reliable technique is missing for most protozoan taxa, thus demonstration of the causative agent in native material or stained smears is still the gold standard for detection. (els.net)
Bacteria4
- defence against bacteria.Duality of Immune System I. . antibodies and are called plasma cells. (scribd.com)
- Bacteria and viruses that are inside host cells and are inaccessible to antibodies. (scribd.com)
- We examine selected extracellular and intracellular bacteria, viruses and protozoa, to explore the similarities and differences of vector/pathogen/host interactions among groups of microorganisms. (hhmi.org)
- Macrophage white blood cells engulf such organisms, which include bacteria and protozoa. (sciencephoto.com)
Plasmodium3
- There are no publications for Plasmodium Falciparum Schizont Infected RBCs Antibody (NB100-64491PCP). (novusbio.com)
- Be the first to review our Plasmodium Falciparum Schizont Infected RBCs Antibody (11B7) [PerCP] and receive a gift card or discount. (novusbio.com)
- Finally, we study Plasmodium protozoa, which cause malaria. (hhmi.org)
Leishmania4
- Prevalence of agglutinating anti-Leishmania antibodies in two multi-thousand Bengoli communities. (nih.gov)
- Moreover, 2 to 5 mL of blood was taken from each of the rodents and the sera were examined for anti- Leishmania antibodies, by ELISA, or anti- T. gondii antibodies, by modified agglutination test (MAT). (hindawi.com)
- Canine visceral leishmaniasis (CVL) is caused by the protozoan Leishmania infantum , which infects dogs and humans in many regions of Brazil . (bvsalud.org)
- Four monoclonal antibodies (McAbs) were generated against the soluble extracellular acid phosphatase (EC 3.1.3.2) (S-AcP) of Leishmania donovani. (lancs.ac.uk)
Proteins4
- Search, Find and Buy Antibodies, ELISA Kits and Proteins. (antibodies-online.com)
- Most salivary proteins (SPs) are highly immunogenic, able to elicit antibody production and memory responses ( 15 , 16 ). (frontiersin.org)
- Produce, clone, and cryopreserve, immortal rmurine hybridomos secreting IgG class monoclonal antibodies (MAB) which bind to P marinus cell walls and soluble proteins. (umd.edu)
- We used anti-cyclin-A antibody (human origin) in western blot assay with epimastigote of T. cruzi proteins and this recognized a ~ 55 kDa polypeptide. (scirp.org)
Secondary Antibodies1
- Without primary antibodies (whether polyclonal or monoclonal), the opportunity for further research involving secondary antibodies is non-existent. (prosci-inc.com)
Primary antibody5
- Thanks to years of painstaking antibody research , ProSci has created an impressive primary antibody production catalog of research-ready primary antibodies for a wide range of clinical applications. (prosci-inc.com)
- Our primary antibody selection can benefit your research endeavors, no matter your targeted study area. (prosci-inc.com)
- We're researchers ourselves, with years of lab and clinical experience backing our primary antibody production catalog. (prosci-inc.com)
- ProSci's primary antibody product line offers a wide-ranging selection of antibodies backed by science - and well-regarded by our growing client base. (prosci-inc.com)
- Call a ProSci primary antibody expert at (888) 513-9525, or visit our contact page for department-based email contacts and a secure message portal. (prosci-inc.com)
ELISA3
- The antibody can be used for easy quantification of in vitro cell proliferation by ELISA and has successfully been used for monitoring cytotoxity and apoptosis as well as ELISA-based co-culture angiogenesis and proliferation assays. (abcam.com)
- This antibody may be used in combination with clone MP5-32C11 (OASA05651) in sandwich ELISA and ELISPOT assays. (avivasysbio.com)
- We have available a DIY ELISA protocol for detection of HIV-1 gp120env incorporating code D7324 as the capture antibody. (linscottsdirectory.com)
Antigen3
- Serum containing antibodies to a specific antigen(s). (scribd.com)
- Results were negative for serum anti-A IgM, antibody to hepatitis B core antigen (anti-HBc), antibody to hepatitis B surface antigen (anti-HBs), and antibody to hepatitis C virus (anti-HCV). (cdc.gov)
- 2. Purified antibodies or antigen-binding fragments of said antibodies raised against purified extracellular membrane vesicles exported from Borrelia burgdorferi. (freepatentsonline.com)
Infection11
- Mice receiving the treatment produced their own monoclonal antibodies and survived infection with the life-threatening pathogen Pseudomonas aeruginosa . (the-scientist.com)
- The current study aimed to assess the protozoan infection of rodents in Boyer-Ahmad district, southwestern Iran. (hindawi.com)
- Journal Article] Effects of a transferring antibody against Neospora caninum infection in a murine model. (nii.ac.jp)
- The prevalence of antibodies against cytomegalovirus (CMV) and the incidence of CMV infection were tested in 98 children aged 5 to 36 months who attended the day-care center of a University hospital in São Paulo. (scielo.br)
- Antibodies that induce phagocytosis of malaria infected erythrocytes: effect of HIV infection and correlation with clinical outcomes. (nih.gov)
- In women with placental malaria infection, phagocytic antibodies to CS2(VSA) were decreased in the presence of HIV (p = 0.011) and correlated positively with infant birth weight (coef = 3.57, p = 0.025), whereas total IgG to CS2(VSA) did not. (nih.gov)
- Effect of HIV on the anti-CS2VSA antibody levels in the 'No Malaria' histopathology group.In a univariate analysis of women that showed no evidence of either peripheral or placental malaria infection, using Mann-Whitney ranksum tests, phagocytic antibodies (A) are shown to be significantly decreased by HIV infection (z = −2.17, p = 0.03). (nih.gov)
- Babesia serological testing is used to diagnose infection by the Babesia tick-borne protozoan. (questdiagnostics.com)
- The American College of Physicians recognizes that blood tests… "cannot diagnose Lyme disease alone, but they are used to confirm a diagnosis," and that "anti-Borrelia burgdorferi antibodies may take up to 2 to 6 weeks after infection to appear in the blood. (google.com)
- Toxoplasma gondii infection is a protozoan infection to which most adults have antibody protection. (health.am)
- serologic testing for specific antibodies to T cruzi is the cornerstone of diagnosing chronic T cruzi infection (Kirchhoff, et al. (bartleby.com)
Diagnosis2
- Diagnosis of EPM is based upon finding antibodies or, more recently, a DNA detection test of either blood or cerebrospinal fluid. (horses-and-horse-information.com)
- The invention further relates to kits, for the diagnosis of Lyme disease, comprising the antibodies and ancillary reagents. (freepatentsonline.com)
IMMUNOGEN3
- The details of the immunogen for this antibody are not available. (abcam.com)
- IgY antibodies are first obtained from the egg of a domestic fowl hen which has been actively immunized against said one or more pathogenic organisms by injection with an immunogen containing immunogenic determinants specific to elicit such antibodies. (google.it)
- 1. A method for treating systemic manifestations of an infectious non- intestinal disease associated with pathogenic organisms or molecules in a mammal, said method comprising orally administering to said mammal IgY antibodies obtained from the egg of a domestic fowl hen which has been actively immunized against said pathogenic organisms by injection of the hen with an immunogen containing immunogenic determinants specific to elicit said antibodies. (google.it)
Production of antibodies1
- The immune response induced by arthropod saliva is mainly Th2, which favors the production of antibodies ( 20 , 21 ). (frontiersin.org)
Immunofluorescence3
- Immunofluorescence microscopy with an anti-EF-Tu polyclonal antibody showed that Colpoda EF-Tu (p45) was localized in the lepidosomes. (antibody-antibodies.com)
- This antibody has been shown to be useful, using immunofluorescence, in the detection of dsDNA in fx. (abcam.com)
- The feces were examined by coprological techniques (staining with iron hematoxylin, Ziehl-Neelsen, and modified Weber's chromotrope 2R-based trichrome), and with immunofluorescence antibody MERIFLUOR Cryptosporidium/Giardia assay. (pubmedcentralcanada.ca)
Enzyme-linke1
- One hundred forty-eight women with high-risk pregnancies who had abnormal pregnancy outcomes (cases) and 100 with normal pregnancies (controls) were tested for the presence of Toxoplasma DNA in their blood by a nested PCR and specific antibodies to Toxoplasma by an enzyme-linked immunosorbent assay. (ajtmh.org)
Malaria2
- Here, we provide background information and present our hypothesis that IgG4 may not only be a useful tool to measure exposure to infected mosquito bites, but that these bi-specific antibodies may also play an important role in modulation of the immune response against malaria and other vector-borne diseases in endemic settings. (frontiersin.org)
- Of the around 100 protozoan species that can infect humans, some are of prime importance for human health, including the causative agents of malaria, amebiasis, leishmaniasis and sleeping sickness . (els.net)
Intestinal protozoa1
- van Lieshout L and Verweij JJ (2010) Newer diagnostic approaches to intestinal protozoa. (els.net)
Microorganisms1
- The development of resistance and susceptibility of microorganisms to their specific antibodies. (indiana.edu)
Humans1
- Many parasitic protozoa infect only humans without adequate immunological defenses although a few infect the immunocompetent. (thefreedictionary.com)
Organisms4
- IgY antibodies are first obtained from the egg of a domestic fowl hen which has been actively immunized against said one or more pathogenic organisms. (google.it)
- The antibodies are then administered orally to a mammal suffering from an infectious systemic disease caused or exacerbated by such pathogenic organism or organisms. (google.it)
- The method of claim 1, wherein at least one of said one or more pathogenic organisms is a protozoan. (google.it)
- It was called spinal segmental myelitis until clusters of protozoa (single myelitis until clusters of protozoa (single celled organisms were found around the affected nerves. (horses-and-horse-information.com)
Isotype1
- The anti- T. cruzi epimastigote stage antibody isotype profiles in the sera of Brazilian patients from each clinical category, as well as from uninfected individuals (UNINF) from the same endemic area were analyzed. (ajtmh.org)
Detection1
- Three blood cultures as well as serologic investigations for the detection of antibodies to Entamoeba histolytica, performed on day 3 and 7 after the onset of clinical symptoms, remained negative. (curezone.org)
Infections2
- Opportunistic protozoan infections caused by Cryptosporidium parvum and Toxoplasma gondii are seen in patients who are immunosuppressed by disease or drug therapy. (thefreedictionary.com)
- Several protozoan infections show a more severe progression in the immunocompromised host , important examples are toxoplasmosis, pneumocystosis, cryptosporidiosis and visceral leishmaniasis. (els.net)
Cryptosporidium1
- The present study investigated the presence of dispersive stages of potentially zoonotic protozoans belonging to the genera Blastocystis , Giardia and Cryptosporidium , and Microsporidia spores in feces collected from birds inhabiting the breeding colony established at one lake island in Poland, Europe. (pubmedcentralcanada.ca)
Giardia3
- Giardia lamblia Antibody Cat. (prosci-inc.com)
- Giardia lamblia antibody is in a phosphate saline buffer (0.01M, pH 7.2) containing 0.1% sodium azide preservative. (prosci-inc.com)
- Giardia lamblia antibody can be stored at -20˚C, stable for one year. (prosci-inc.com)
Humoral1
- Autoinmunidad humoral en la enfermedad de Chagas cronica : anticuerpos contra las proteinas ribosomales P. / Gabriela Laura Levitus. (who.int)
Recombinant1
- Antibodies directed to purified recombinant variable domain of Tpr K can opsonize T. pallidum, Nichols strain, for phagocytosis, supporting the hypothesis that this portion of the protein is exposed at the surface of the treponeme. (nih.gov)
Extracellular1
- Antibodies are found in extracellular fluids (blood plasma. (scribd.com)
Cruzi1
- This is the first report of antibody-delivery into viable epimastigote forms of T. cruzi , with a simple and cheap technique, which will allows carrying out further studies of this protozoan. (scirp.org)
GENTAUR1
- GENTAUR antibody-antibodies.com The Marketplace for Antibodies : Morphogenetic and molecular analyses of cyst wall components in the ciliated protozoan Colpoda cucullus Nag-1. (antibody-antibodies.com)
Toxoplasma1
- Effect of the strain of Toxoplasma gondii on the development of toxoplasmic encephalitis in mice treated with antibody to interferon-gamma. (nih.gov)
Cysts1
- Of special concern are macroscopic tapeworm cysts and microscopic cysts of protozoa. (fda.gov)
Inhibit1
- Cows injected with a protein that mimics HIV's envelope make broadly neutralizing antibodies that inhibit multiple strains of the virus. (the-scientist.com)
Prevalence1
- At the beginning of the study the overall prevalence of anti-CMV IgG antibodies was 44% (43/98). (scielo.br)
Titers2
- We will additionally measure Total IgG antibody titers in the blood, and we will compare groups using logged values of the antibody levels. (clinicaltrials.gov)
- Blood antibody titers, however, should not be considered evidence of the clinical disease. (horses-and-horse-information.com)
Salivary1
- During transmission of most vector-borne diseases, the pathogen is delivered to the vertebrate host along with the arthropod saliva during blood feeding and previous studies have shown the existence of IgG4 antibodies against mosquito salivary allergens. (frontiersin.org)
Disease5
- These data suggest the presence of complex immunoregulatory processes, most likely related to differential cytokine involvement, which can influence the expression of antibody isotypes and possibly the course of disease. (ajtmh.org)
- Here, we present a brief review on the antibody response against SPs and the hypothetical implication of IgG4 antibodies in disease progression of several vector-borne diseases. (frontiersin.org)
- Broadly, the present invention is directed to the use of egg antibody preparations in the treatment of systemic disease in human and non-human mammals. (google.it)
- Equine protozoa myelitis (which, fortunately, is shortened to EPM), is a common neurological disease. (horses-and-horse-information.com)
- CDC also first recognized New Jersey as endemic for Babesiosis , another tickborne disease caused by a protozoa, in 2002. (google.com)
Diagnostic1
- Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery. (abcam.com)
Ciliate3
- In the ciliate protozoan Tetrahymena thermophila , a wide variety of programmed rearrangements occur during the development of the somatic nucleus including chromosome fragmentation and deletion of specific DNA sequences. (els.net)
- The ciliate protozoa undergo large‐scale programmed DNA rearrangements during nuclear development. (els.net)
- Philasterides dicentrarchi is a marine protozoan ciliate that was first identified in 1995 after being isolated from infected European sea bass (Dicentrarchus labrax) reared in France. (wikipedia.org)
Waterborne1
- 14. Risk assessment of waterborne protozoa: current status and future trends. (indigo.ca)
Specific1
- Chronic exposure to mosquito bites induces higher IgG4 antibodies against SPs than other IgG subclasses ( 22 , 26 , 27 ), suggesting that this specific antibody subclass may be a marker of intense exposure to arthropod vectors ( 28 , 29 ). (frontiersin.org)
Serum1
- Antiserum: Antiserum: Serology: Serology: Gamma Globulins: Globulins: Fraction of serum that contains most of the antibodies. (scribd.com)
Human4
- Further, none of the antibodies immunoprecipitated any of the five human acid phosphatase isozymes or reacted with them in Western blots or the enzyme activity binding assay. (lancs.ac.uk)
- We have generated high-affinity single-chain antibody fragment (scFv) probes for the guanine-quadruplex formed by the Stylonychia telomeric repeat, by ribosome display from the Human Combinatorial Antibody Library. (pnas.org)
- V(D)J recombination is a DNA rearrangement that occurs during the development of the human immune system to assemble functional genes encoding antibodies. (els.net)
- V(D)J recombination in human development assembles functional genes encoding antibodies. (els.net)
Immune4
- This is due to the fact that, although IgG4 may act as a blocking antibody, it is not efficient in forming large immune complexes ( 30 ). (frontiersin.org)
- Treponema pallidum major sheath protein homologue Tpr K is a target of opsonic antibody and the protective immune response. (nih.gov)
- Individuals who have sex once or twice a week show 30 percent higher levels of an antibody called immunoglobulin A, which is known to boost the immune system," he says. (valleyforward.org)
- Immune-mediated thrombocytopenia (ITP) is one of the most common causes of thrombocytopenia in dogs where the dog's immune system produces antibodies which attack and destroy the blood cells. (justanswer.com)
Molecular3
- Morphogenetic and molecular analyses of cyst wall components in the ciliated protozoan Colpoda cucullus Nag-1. (antibody-antibodies.com)
- A considerable part of protozoan diagnostics still mainly relies on microscopy, however, in the past years molecular methods, particularly polymerase chain reaction (PCR)‐based techniques, have gained more and more importance. (els.net)
- In many protozoan taxa, morphology alone does not provide enough information for identification on or below the species level, this is today mainly based on molecular biological methods . (els.net)
Vaccine1
- Surprisingly, antibodies elicited by the vaccine enter the tick when the arthropod feeds, and destroy OspA-producing B. burgdorferi in the vector. (hhmi.org)
Gondii2
- Antibodies to T. gondii were detected in the sera of 5 (9.61%) cases. (hindawi.com)
- The seroprevalence rates of anti-L. infantum, anti- Ehrlichia , anti-B. canis, anti-T. gondii and anti-N. caninum antibodies were 37.6%, 75.3%, 72%, 47.3% and 6.4%, respectively. (bvsalud.org)