GlucuronidaseElectrophoresis, Cellulose Acetate: Electrophoresis in which cellulose acetate is the diffusion medium.Mucopolysaccharidosis VII: Mucopolysaccharidosis characterized by excessive dermatan and heparan sulfates in the urine and Hurler-like features. It is caused by a deficiency of beta-glucuronidase.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Lysosomes: A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured. Such rupture is supposed to be under metabolic (hormonal) control. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)Antibody Specificity: The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.Mice, Inbred Strains: Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.Antibodies, Viral: Immunoglobulins produced in response to VIRAL ANTIGENS.Antibodies, Bacterial: Immunoglobulins produced in a response to BACTERIAL ANTIGENS.Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Kidney: Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.Testosterone: A potent androgenic steroid and major product secreted by the LEYDIG CELLS of the TESTIS. Its production is stimulated by LUTEINIZING HORMONE from the PITUITARY GLAND. In turn, testosterone exerts feedback control of the pituitary LH and FSH secretion. Depending on the tissues, testosterone can be further converted to DIHYDROTESTOSTERONE or ESTRADIOL.Antibody Formation: The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.Antibodies, Neutralizing: Antibodies that reduce or abolish some biological activity of a soluble antigen or infectious agent, usually a virus.Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.Genes: A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.Antibody Affinity: A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes.Fluorescent Antibody Technique: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.Antibodies, Anti-Idiotypic: Antibodies which react with the individual structural determinants (idiotopes) on the variable region of other antibodies.Binding Sites, Antibody: Local surface sites on antibodies which react with antigen determinant sites on antigens (EPITOPES.) They are formed from parts of the variable regions of FAB FRAGMENTS.HIV Antibodies: Antibodies reactive with HIV ANTIGENS.Epitopes: Sites on an antigen that interact with specific antibodies.Antibodies, Neoplasm: Immunoglobulins induced by antigens specific for tumors other than the normally occurring HISTOCOMPATIBILITY ANTIGENS.Antibodies, Protozoan: Immunoglobulins produced in a response to PROTOZOAN ANTIGENS.Antibodies, Antinuclear: Autoantibodies directed against various nuclear antigens including DNA, RNA, histones, acidic nuclear proteins, or complexes of these molecular elements. Antinuclear antibodies are found in systemic autoimmune diseases including systemic lupus erythematosus, Sjogren's syndrome, scleroderma, polymyositis, and mixed connective tissue disease.Cross Reactions: Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.Procollagen: A biosynthetic precursor of collagen containing additional amino acid sequences at the amino-terminal and carboxyl-terminal ends of the polypeptide chains.Liposomes: Artificial, single or multilaminar vesicles (made from lecithins or other lipids) that are used for the delivery of a variety of biological molecules or molecular complexes to cells, for example, drug delivery and gene transfer. They are also used to study membranes and membrane proteins.Immunoassay: A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.Antigen-Antibody Complex: The complex formed by the binding of antigen and antibody molecules. The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES.Patents as Topic: Exclusive legal rights or privileges applied to inventions, plants, etc.Acute-Phase Reaction: An early local inflammatory reaction to insult or injury that consists of fever, an increase in inflammatory humoral factors, and an increased synthesis by hepatocytes of a number of proteins or glycoproteins usually found in the plasma.Complement System Proteins: Serum glycoproteins participating in the host defense mechanism of COMPLEMENT ACTIVATION that creates the COMPLEMENT MEMBRANE ATTACK COMPLEX. Included are glycoproteins in the various pathways of complement activation (CLASSICAL COMPLEMENT PATHWAY; ALTERNATIVE COMPLEMENT PATHWAY; and LECTIN COMPLEMENT PATHWAY).Phosphotyrosine: An amino acid that occurs in endogenous proteins. Tyrosine phosphorylation and dephosphorylation plays a role in cellular signal transduction and possibly in cell growth control and carcinogenesis.Hybridomas: Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell.Tyrosine: A non-essential amino acid. In animals it is synthesized from PHENYLALANINE. It is also the precursor of EPINEPHRINE; THYROID HORMONES; and melanin.Stretchers: Bed-like structures for transporting or temporarily holding patients.Neoplasms: New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.Maytansine: An ansa macrolide isolated from the MAYTENUS genus of East African shrubs.Polyethylene Glycols: Polymers of ETHYLENE OXIDE and water, and their ethers. They vary in consistency from liquid to solid depending on the molecular weight indicated by a number following the name. They are used as SURFACTANTS, dispersing agents, solvents, ointment and suppository bases, vehicles, and tablet excipients. Some specific groups are NONOXYNOLS, OCTOXYNOLS, and POLOXAMERS.Immunoconjugates: Combinations of diagnostic or therapeutic substances linked with specific immune substances such as IMMUNOGLOBULINS; MONOCLONAL ANTIBODIES; or ANTIGENS. Often the diagnostic or therapeutic substance is a radionuclide. These conjugates are useful tools for specific targeting of DRUGS and RADIOISOTOPES in the CHEMOTHERAPY and RADIOIMMUNOTHERAPY of certain cancers.MaleimidesDrug Delivery Systems: Systems for the delivery of drugs to target sites of pharmacological actions. Technologies employed include those concerning drug preparation, route of administration, site targeting, metabolism, and toxicity.Phenolsulfonphthalein: Red dye, pH indicator, and diagnostic aid for determination of renal function. It is used also for studies of the gastrointestinal and other systems.Schools, Medical: Educational institutions for individuals specializing in the field of medicine.Blood Banks: Centers for collecting, characterizing and storing human blood.Dermatology: A medical specialty concerned with the skin, its structure, functions, diseases, and treatment.Calcium Phosphates: Calcium salts of phosphoric acid. These compounds are frequently used as calcium supplements.Diploidy: The chromosomal constitution of cells, in which each type of CHROMOSOME is represented twice. Symbol: 2N or 2X.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Chlorpheniramine: A histamine H1 antagonist used in allergic reactions, hay fever, rhinitis, urticaria, and asthma. It has also been used in veterinary applications. One of the most widely used of the classical antihistaminics, it generally causes less drowsiness and sedation than PROMETHAZINE.Clotrimazole: An imidazole derivative with a broad spectrum of antimycotic activity. It inhibits biosynthesis of the sterol ergostol, an important component of fungal CELL MEMBRANES. Its action leads to increased membrane permeability and apparent disruption of enzyme systems bound to the membrane.Acyclovir: A GUANOSINE analog that acts as an antimetabolite. Viruses are especially susceptible. Used especially against herpes.Histamine H1 Antagonists: Drugs that selectively bind to but do not activate histamine H1 receptors, thereby blocking the actions of endogenous histamine. Included here are the classical antihistaminics that antagonize or prevent the action of histamine mainly in immediate hypersensitivity. They act in the bronchi, capillaries, and some other smooth muscles, and are used to prevent or allay motion sickness, seasonal rhinitis, and allergic dermatitis and to induce somnolence. The effects of blocking central nervous system H1 receptors are not as well understood.Drug Interactions: The action of a drug that may affect the activity, metabolism, or toxicity of another drug.Receptors, Histamine H1: A class of histamine receptors discriminated by their pharmacology and mode of action. Most histamine H1 receptors operate through the inositol phosphate/diacylglycerol second messenger system. Among the many responses mediated by these receptors are smooth muscle contraction, increased vascular permeability, hormone release, and cerebral glyconeogenesis. (From Biochem Soc Trans 1992 Feb;20(1):122-5)Butyrophenones: Compounds containing phenyl-1-butanone.
... a chimeric antibody produced in a hybridoma. Epicyte - spermicidal antibodies in corn Epicyte was purchased by Biolex in 2004 ... ProdiGene struck agreements with Sigma to distribute ProdiGene's corn-produced aprotinin, trypsin, beta-glucuronidase (GUS), ... antibodies against Streptococcus mutans, antibodies against doxorubicin, and ICAM 1 receptor in tobacco PlantForm Corporation ... The product under development, ALT-836, formerly known as TNX-832 and Sunol-cH36, is the not the plant-produced antibody, but ...
... beta glucuronidase (p-ANCA pattern), azurocidin (p and c-ANCA pattern), actin (p and a-ANCA) and h-lamp-2 (c-ANCA). Enzyme- ... Classical p-ANCA occurs with antibodies directed to MPO. p-ANCA without nuclear extension occurs with antibodies to BPI, ... Sinclair, D; Stevens, JM (Sep 2007). "Role of antineutrophil cytoplasmic antibodies and glomerular basement membrane antibodies ... Anti-neutrophil cytoplasmic antibodies (ANCAs) are a group of autoantibodies, mainly of the IgG type, against antigens in the ...
... s express a high-affinity receptor (FcεRI) for the Fc region of IgE, the least-abundant member of the antibodies. This ... lysosomal enzymes β-hexosaminidase β-glucuronidase arylsulfatases newly formed lipid mediators (eicosanoids): thromboxane ... As a result, mast cells are coated with IgE, which is produced by plasma cells (the antibody-producing cells of the immune ... binding of antibody or complement-coated bacteria to complement or immunoglobulin receptors, or recognition of endogenous ...
Hess M, Stritzker J, Härtl B, Sturm JB, Gentschev I, Szalay AA (2011). "Bacterial glucuronidase as general marker for oncolytic ... 2009). "Anti-VEGF single-chain antibody GLAF-1 encoded by oncolytic vaccinia virus significantly enhances antitumor therapy". ...
Their innate immune systems do not produce antibodies, and they should seemingly not be able to respond to new challenges ... required activation by skin-associated microbial glucuronidases and sulfatases. In the absence of microbial populations in the ...
It contained three bacterial genes, two CP4 EPSPS genes, and a gene encoding beta-glucuronidase (GUS) from Escherichia coli as ... Jha, Alok (14 August 2012) Julian Ma: I'm growing antibodies in tobacco plants to help prevent HIV The Guardian. Retrieved 12 ... Tobacco plants have been modified to produce therapeutic antibodies. Algae is under development for use in biofuels. ... antibodies and antigens for vaccinations A study led by the European Food Safety Authority (EFSA) found also viral genes in ...
In 1988 the first human antibodies were produced in plants. In 1987, the ice-minus strain of Pseudomonas syringae became the ... beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants". The EMBO Journal. 6 (13): 3901-07. ISSN ... permanent dead link] Spencer, L; Humphries, J; Brantly, M. (12 May 2005). "Antibody Response to Aerosolized Transgenic Human ...
To do so, it produces a range of enzymes such as lyases, glucuronidases, sulfoesterases, and sulfamidases. The lyases have ... cross-reactivity of danaparoid with heparin-induced antibodies is reported as less than 10%. The effects of heparin are ...
... antibodies labeled with radioactive isotopes for quantifying the levels of IgE antibody in the blood. Other newer methods use ... EPD uses dilutions of allergen and an enzyme, beta-glucuronidase, to which T-regulatory lymphocytes are supposed to respond by ... A rule of thumb is that the higher the IgE antibody value, the greater the likelihood of symptoms. Allergens found at low ... To assess the presence of allergen-specific IgE antibodies, two different methods can be used: a skin prick test, or an allergy ...
In 1988 the first human antibodies were produced in plants.[27] In 1987, the ice-minus strain of Pseudomonas syringae became ... "GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants". The EMBO Journal. 6 (13): ... "Antibody Response to Aerosolized Transgenic Human Alpha1-Antitrypsin". New England Journal of Medicine. 352 (19): 19. PMID ...
It contained three bacterial genes, two CP4 EPSPS genes, and a gene encoding beta-glucuronidase (GUS) from Escherichia coli as ... Jha, Alok (14 August 2012) Julian Ma: I'm growing antibodies in tobacco plants to help prevent HIV The Guardian. Retrieved 12 ... antibodies[57] and antigens for vaccinations[58] A study led by the European Food Safety Authority (EFSA) found also viral ... Tobacco plants have been modified to produce therapeutic antibodies.[147] ...
... rmewing at vax.oxford.ac.uk rmewing at vax.oxford.ac.uk Fri Aug 27 09:57:05 EST ... I am interested in obtaining an antibody to the Beta - glucuronidase enzyme (GUS) for use in maize tissue. Any offers / ...
... Joseph Walker jmwalke1 at FACSTAFF.WISC.EDU Thu Jul 13 07:41:41 EST 1995 *Previous ... If anyone knows of a supply of good anti B-glucuronidase antibodies please contact me at: jmwalke1 at facstaff.wisc.edu * ... I am looking for antibodies against B-glucuronidase to be used in an immunoprecipitation assay. We ran out of our lot and have ...
... antibody validated for WB, IHC. Referenced in 2 publications. Immunogen corresponding to synthetic peptide ... Primary antibodies. Secondary antibodies. ELISA, Matched Antibody Pairs and Multiplex Immunoassays. Cell and tissue imaging ... Custom antibody development. We offer world-leading antibody development platforms based on a proprietary RabMAb® rabbit ... Synthetic peptide corresponding to Escherichia coli Beta Glucuronidase (GUS) aa 1-16 (N terminal) conjugated to Keyhole Limpet ...
Mouse Monoclonal Anti-beta-Glucuronidase/GUSB Antibody (105). Validated: WB, ELISA, IHC, IHC-Fr. Tested Reactivity: Human. 100 ... Home » beta-Glucuronidase/GUSB » beta-Glucuronidase/GUSB Antibodies » beta-Glucuronidase/GUSB Antibody (105) ... beta-Glucuronidase/GUSB Antibody (105) Summary. Immunogen. The antibody reacts with the Beta-Glucuronidase antigen. ... PTMs for beta-Glucuronidase/GUSB Antibody (NBP1-42247). Learn more about PTMs related to beta-Glucuronidase/GUSB Antibody (NBP1 ...
Beta Glucuronidase antibody LS-C700612 is a Cy3-conjugated rabbit polyclonal antibody to rat Beta Glucuronidase (GUSB ). ... Antibodies All Antibodies Primary Antibodies Secondary Antibodies IHC-plus Antibodies PathPlus Antibodies IsoType Control ... Beta Glucuronidase antibody LS-C700612 is a Cy3-conjugated rabbit polyclonal antibody to rat Beta Glucuronidase (GUSB ). ... GUSB / Beta Glucuronidase Antibody (C‑Terminus) LS‑C40459 Species: Human, Mouse, Bat, Guinea pig, Horse, Rabbit, Chicken, ...
Here, we investigated if local expression of β-glucuronidase (βG) on cancer cells to catalytically convert SN38G to SN38 could ... Surface β-glucuronidase-expressing cells were 20 to 80-fold more sensitive to SN-38G than the parental cells. Intravenous CPT- ... Cells were stained with a rat antibody against murine βG (7G7)23 or a rat antibody against HA (Roche, Mannheim, Germany) ... Biotin-labeled goat anti-HA or biotin-labeled goat anti-rabbit immunoglobulin G (control antibody) antibodies were applied to ...
BETA GLUCURONIDASE/GUSB information: Molecular Weight: 74732 MW; Subcellular Localization: Lysosome. ... Polyclonal antibody for BETA GLUCURONIDASE/GUSB detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive ... Western Blot Antibodies. IHC Antibodies. Flow Cytometry Antibodies ChIP Antibodies Secondary Antibodies. -HRP Conjugated. - ... Human Antibodies&ELISA. Mouse Antibodies&ELISA. Rat Antibodies&ELISA. Cow/Bovine Antibodies&ELISA. Pig/Porcine Antibodies&ELISA ...
Haisma, HJ, Boven, E, Van Muijen, M, De Jong, J, Van der Vijgh, WJF & Pinedo, HM 1992, A monoclonal antibody-β-glucuronidase ... A monoclonal antibody-β-glucuronidase conjugate as activator of the prodrug epirubicin-glucuronide for specific treatment of ... A monoclonal antibody-β-glucuronidase conjugate as activator of the prodrug epirubicin-glucuronide for specific treatment of ... A monoclonal antibody-β-glucuronidase conjugate as activator of the prodrug epirubicin-glucuronide for specific treatment of ...
A homogeneous phase reaction occurs with the antibody and complement acting to release the enzyme if an immunospecific antigen- ... antibody complex is formed at the surface of the liposome. The positions of the antigen and antibody can be reversed. ... enzyme encapsulating liposomes which are immunospecifically ruptured in the presence of cognate antibody and active complement ... β-glucuronidase. 3.4 Acting on peptide bonds. 3.4.2 Peptidyl-amino acid hydrolase ...
Atypical STEC O157:H7 strains showing a β-glucuronidase-positive phenotype (GP STEC O157:H7) have rarely been isolated from ... Major virulence genes are shared between typical (β-glucuronidase-negative) and GP STEC O157:H7 strains, and the Stx2-producing ... coated with capture antibodies that recognize both Stx1 and Stx2. We conjugated monoclonal antibodies against Stx1 and Stx2 ( ... The uidA gene, which encodes β-glucuronidase, was intact in all the GP STEC O157:H7 strains sequenced in this study, as well as ...
Primary & Secondary Antibody Conjugates. Primary Antibodies , Secondary Antibodies Search with Antibody Finder ... Antibody & Protein Labeling Kits. Protein Labeling Kits , Small Ligand Labeling Mix-n-Stain™ Antibody Labeling Kits ... Antibody Finder & Advanced SearchBrochures & Safety ReportsFAQsNew! Biotium Insider BlogPublicationsSpectra ViewerTech Tips ... X-GlcU (5-Bromo-4-chloro-3-indolyl-β-D-glucuronide, cyclohexylammonium salt) is a chromogenic β-glucuronidase substrate widely ...
In addition, the antibodies have potential uses in diagnosis of a variety of diseases, including certain cancers. The ... antibodies, which have demonstrated affinity for a variety of molecules containing o-phosphotyrosine residues, were prepared ... A hybridoma cell line is disclosed that secretes monoclonal antibodies which serve as a high titer, reproducible, biological ... Microbial B-glucuronidase genes, gene production and uses thereof. US20030191283 *. 31 Jan 2002. 9 Oct 2003. Genentech, Inc.. ...
Beta-glucuronidase-mediated drug release. Curr Pharm Des 2002;8:1391-403. ... Antibody-drug conjugates (ADC) seek to combine the exquisite specificity of an antibody to its cognate antigen with the ... Pharmocokinetic experiments were performed using radiolabeled antibodies and ADCs. To a solution of antibody or ADC in PBS ... Minor groove binder antibody conjugates employing a water soluble beta-glucuronide linker. Bioorg Med Chem Lett 2007;17:2278-80 ...
Galactosylated antibodies and antibody-enzyme conjugates in antibody-directed enzyme prodrug therapy. Cancer 1994;73:1114-20. ... β-Glucuronidase-mediated drug release. Curr Pharm Des 2002;8:1391-403. ... Mouse Model for Antibody Responses. Female CB6F1 mice were immunized with 20 μg/mL β-lactamase or β-lactamase variant i.p. with ... Antibody-directed enzyme prodrug therapy with the T268G mutant of human carboxypeptidase A1: in vitro and in vivo studies with ...
Rabbit Polyclonal Anti-Cathepsin D Antibody. Lysosomes Marker. Validated: IHC, IHC-P. Tested Reactivity: Human. 100% Guaranteed ... Reviews for Cathepsin D Antibody (NB120-826) (0) There are no reviews for Cathepsin D Antibody (NB120-826). By submitting a ... Diseases for Cathepsin D Antibody (NB120-826). Discover more about diseases related to Cathepsin D Antibody (NB120-826). ... PTMs for Cathepsin D Antibody (NB120-826). Learn more about PTMs related to Cathepsin D Antibody (NB120-826). ...
β-glucuronidase served as internal standard. Bottom, immunohistochemical staining of hASH2 of a kidney carcinoma (renal cell; ... For control, the blots were developed with an antibody specific for actin. B, tumor and normal tissue from the same biopsy were ... The reactions were standardized for β-glucuronidase expression. The signal from primary HDF was artificially set as 1. BT483 ... Monoclonal antibodies (mAb) 4C5 and 6A9 were generated by immunizing a LOU/C rat with bacterially expressed glutathione S- ...
1982) 2 1044-51), β-glucuronidase, peroxidase, β-lactamase (U.S. Pat. Nos. 5,741,657 and 5,955,604), catalytic antibodies, ... such as an antibody or antibody fragment or a protein comprising an antibody fragment, or a genetic construct which acts at the ... LOX-1 inhibition via blocking antibodies or antisense technology is associated with attenuation of sepsis, heart failure, ... via a europium-tagged anti-GST antibody, and the coactivator peptide is labeled with allophycocyanin via a streptavidin-biotin ...
Hemlibra Hemlibra (emicizumab-kxwh) is a bispecific factor IXa- and factor X-directed antibody indicated to... ... Mepsevii Mepsevii (vestronidase alfa) is a recombinant human lysosomal beta glucuronidase indicated for the... ... Fasenra Fasenra (benralizumab) is an interleukin-5 receptor alpha-directed cytolytic monoclonal antibody... ...
Hemlibra Hemlibra (emicizumab-kxwh) is a bispecific factor IXa- and factor X-directed antibody indicated to... ... Mepsevii Mepsevii (vestronidase alfa) is a recombinant human lysosomal beta glucuronidase indicated for the... ... Fasenra Fasenra (benralizumab) is an interleukin-5 receptor alpha-directed cytolytic monoclonal antibody... ...
Antibody detection by enzyme-linked immunosorbent assay (ELISA). Total IgA and IgG2b antibody titres in blood serum and ... g) IL-17A expression relative to the housekeeping gene β-glucuronidase (GUSB) as determined in small intestinal tissue samples ... IgG2b antibodies. (e) IgA antibody levels in small intestinal tissue explant supernatants. (f,g) pIgR and TGF-β mRNA expression ... or with anti-CD3/CD28 antibodies as a positive control. IL-17A responses triggered by anti-CD3/CD28 antibodies did not differ ...
Anti-C5a antibodies also significantly attenuated the release of the lysosomal enzyme beta-D-glucuronidase from ZAS-stimulated ... such as antineutrophil cytoplasmic antibodies, antinuclear antibodies, and antiglomerular basement membrane antibodies, may ... Control (I), septic (II) and septic + anti-C5a antibody (III) groups (n = 4) were studied. The antibody-treated group (III) ... The antibody to human C5a was thus shown to be cross-reactive with primate C5a and was specific since C3a and C4a levels were ...
Anti-C5a antibodies also significantly attenuated the release of the lysosomal enzyme beta-D-glucuronidase from ZAS-stimulated ... such as antineutrophil cytoplasmic antibodies, antinuclear antibodies, and antiglomerular basement membrane antibodies, may ... Control (I), septic (II) and septic + anti-C5a antibody (III) groups (n = 4) were studied. The antibody-treated group (III) ... The antibody to human C5a was thus shown to be cross-reactive with primate C5a and was specific since C3a and C4a levels were ...
... glucuronidase, beta; KPLPO, ribosomal protein, large, P0; TFRC, transferrin receptor (p90, CD71).. Analytical validation of the ... antigen identified by monoclonal antibody Ki-67; MYBL2, v-myb myeloblastosis viral oncogene homolog (avian)-like 2; PGR, ...
... or are detected by specific antibodies. Enzyme reporters, such as β-glucuronidase (GUS; Jefferson, 1987; Kertbundit et al., ... or of specific antibodies, requires labor-intensive and time-consuming techniques like immunocytochemistry and electron ... Analysis of T-DNA-mediated translational beta-glucuronidase gene fusions. Plant Mol Biol 36: 205-217. ...
The enzyme encoded by this gene has moderate glucuronidase activity with phenols. [provided by RefSeq, Jul 2008] ... antibodies-online: Search results for 6 available UGT1A7 Antibodies ranked by validation data ... antibodies-online: Search results for 6 available UGT1A7 Antibodies ranked by validation data ... antibodies-online: Search results for available UGT1A7 related products ranked by validation data ...
  • The emergence of antibody-drug conjugates (ADC), such as brentuximab vedotin and ado-trastuzumab emtansine, has led to increased efforts to identify new payloads and develop improved drug-linker technologies. (aacrjournals.org)
  • Most antibody payloads impart significant hydrophobicity to the ADC, resulting in accelerated plasma clearance and suboptimal in vivo activity, particularly for conjugates with high drug-to-antibody ratios (DAR). (aacrjournals.org)
  • We recently reported on the incorporation of a discrete PEG 24 polymer as a side chain in a β-glucuronidase-cleavable monomethylauristatin E (MMAE) linker to provide homogeneous DAR 8 conjugates with decreased plasma clearance and increased antitumor activity in xenograft models relative to a non-PEGylated control. (aacrjournals.org)
  • Antileukocyte antibodies in sera from patients with systemic lupus erythematosus (SLE) were characterized by determining cross-reacting specificies with the antigens defined by OKT3, OKT4, OKT8, OKM1 and anti-Ia hybridoma antibodies (Abs). (jci.org)
  • Anti-neutrophil cytoplasmic antibodies (ANCAs) are a group of autoantibodies, mainly of the IgG type, against antigens in the cytoplasm of neutrophil granulocytes (the most common type of white blood cell) and monocytes. (wikipedia.org)
  • GS-ANA are antibodies directed to granulocyte specific nuclear antigens. (wikipedia.org)
  • Other less common antigens include HMG1 (p-ANCA pattern), HMG2 (p-ANCA pattern), alpha enolase (p and c-ANCA pattern), catalase (p and c-ANCA pattern), beta glucuronidase (p-ANCA pattern), azurocidin (p and c-ANCA pattern), actin (p and a-ANCA) and h-lamp-2 (c-ANCA). (wikipedia.org)
  • Although IF can be used to screen for many ANCAs, ELISA is used to detect antibodies to individual antigens. (wikipedia.org)
  • Creative Diagnostics is a leading manufacturer and supplier of antibodies, viral antigens, innovative diagnostic components and critical assay reagents. (creative-diagnostics.com)
  • The antibodies, which have demonstrated affinity for a variety of molecules containing o-phosphotyrosine residues, were prepared using a synthetic analog, p-azobenzyl phosphonate (ABP) covalently linked to a carrier protein, as the antigen. (google.ca)
  • However, all patients formed antibodies against the protein components that limited the number of treatment cycles. (aacrjournals.org)
  • MitoTracker® Red CMXRos, SYTOX® Green nucleic acid stain, biotin-XX goat anti-mouse IgG antibody and Cascade Blue® NeutrAvidin biotin-binding protein. (thermofisher.com)
  • Monoclonal anti-heparanase antibody 1E1 was generated by immunizing Balb/C mice with the entire 65 kDa heparanase protein. (sprentland.com)
  • In order to obtain a stable and reproducible nisin dose-dependent synthesis of a reporter protein (β-glucuronidase) or a model antigen (the C subunit of the tetanus toxin, TTFC), the lactococcal nisRK regulatory genes were integrated into the chromosome of L. plantarum NCIMB8826. (asm.org)
  • The anti-pan carcinoma monoclonal antibody (MAb) 323/A3, linked to E. coli-derived β-glucuronidase (GUS) was used to study the tumour-site-selective activation of the prodrug Epirubicin-glucuronide (Epi-glu). (elsevier.com)
  • X-GlcU (5-Bromo-4-chloro-3-indolyl-β-D-glucuronide, cyclohexylammonium salt) is a chromogenic β-glucuronidase substrate widely used for monitoring E. coli contamination in food (such as meat, dairy products and shellfish, etc) and water, and for the detection of GUS gene expression in plants. (biotium.com)
  • 1999) High cytoplasmic expression in E. coli , purification and in vitro refolding of a single chain Fv antibody fragment against the hepatitis B surface antigen. (springer.com)
  • The characteristics of the interstitial dendritic cell found in heart were studied in detail, and this cell was shown to be negative for acid phosphatase, beta-glucuronidase, and ATPase activity, and certainly some and probably all of the cells were negative for nonspecific esterase activity. (rupress.org)
  • Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies. (lsbio.com)
  • The use of antigenic tags, such as the T7 or HA epitopes, or of specific antibodies, requires labor-intensive and time-consuming techniques like immunocytochemistry and electron microscopy. (plantphysiol.org)
  • Reduced in vivo aortic uptake of radiolabeled oxidation-specific antibodies reflects changes in plaque composition consistent with plaque stabilization. (acris-antibodies.com)
  • Boster also offers various secondary antibodies for Immunoflourescecne and IHC. (bosterbio.com)
  • Biotium offers R-phycoerythrin (R-PE) and Allophycocyanin (APC) labeled streptavidin, Annexin V and a selection of secondary antibodies. (amsthailand.com)
  • Serum antibody responses to i.p. immunisation with chicken gama globulin in BALB/c mice are also modulated by oral application of Lactobacillus. (tudelft.nl)
  • Comparative evaluation of immunoglobulin M neutralizing antibody response in acute-phase sera and virus isolation for the routine diagnosis of enterovirus infection. (asm.org)
  • For researchers wishing to investigate the suitability of our various chromogenic β-glucuronidase substrates, we offer this sampler kit, which contains 5 mg each of the following three different substrates: X-GlcU, CHA ( 10016 ), Red-β-D-GlcU, CHA ( 10018 ), and Rose-β-D-GlcU, CHA ( 10019 ). (biotium.com)
  • An analysis of other lactobacilli identified β-glucuronidase activity and gusA homologs in other L. gasseri isolates but not in other Lactobacillus species tested. (asm.org)
  • Studies with various Lactobacillus species, including L. gasseri , have consistently shown their ability to reduce the amount of fecal β-glucuronidase activity and lower the occurrence of cancer indicators present in the GI tract ( 15 , 27 , 33 , 37 , 41 ). (asm.org)
  • The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. (ptglab.com)
  • The goal of the study presented here was to determine optimal conditions for β-glucuronidase from various species toward hydrolysis of different compound classes with subsequent analysis by UHPLC/MS (TOF). (sigmaaldrich.com)
  • HRP-conjugated goat anti-rabbit antibody was purchased from Jackson ImmunoResearch (Western Grove PA). (sprentland.com)
  • 2002) showed that BM8 antibody is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas and has been shown to be a unique histological marker of the progression from peri-insulitis to ß-cell destruction and diabetes in a mouse diabetes model. (acris-antibodies.com)
  • However, evidence has slowly been accumulating to indicate that β-glucuronidase activity can also be found in a limited number of other bacteria, particularly gram-positive inhabitants of the GI tract ( 3 , 4 , 23 , 36 ). (asm.org)
  • In the intestinal tract, SN-38G is hydrolyzed back to SN-38 through the action of β -glucuronidase produced by the intestinal bacteria [ 9 ]. (hindawi.com)
  • Additionally, bacterial β-glucuronidase activity has been implicated in the generation of toxic and carcinogenic metabolites which may be important precursors to tumor initiation and large bowel cancer in the gut ( 6 , 36 ). (asm.org)
  • To determine whether IL-17A mediates human inflammatory diseases, we investigated the efficacy and safety of AIN457, a human antibody to IL-17A, in patients with psoriasis, rheumatoid arthritis, and chronic noninfectious uveitis. (sciencemag.org)
  • Comparison of methods for the generation of immunoreactive fragments of a monoclonal antibody (B72.3) reactive with human carcinomas. (semanticscholar.org)
  • 2002) Practical considerations for pharmaceutical antibody production in different crop systems. (springer.com)
  • The allergens in this hypersensitivity interact with T lymphocytes (white blood cells of the immune system) rather than antibodies, as in the case of the first type of hypersensitivity. (newworldencyclopedia.org)