Antibodies epitope mapping. Medical search

In all animal species thus far studied, anti-Sm Abs initially recognize proline-rich epitopes in the carboxyl terminus of the Sm-B/B' protein and subsequently to multiple other epitopes in B/B' and D. The absence of appropriate mAbs has limited our understanding of the genetic and structural basis of this autoimmune response. (uthscsa.edu)

Epitope mapping and genetic studies revealed that the anti-Sm response is produced by distinct B cell clones with restricted epitope reactivity. (uthscsa.edu)

On the aggregate, our results demonstrate that combinatorial libraries can recapitulate the immune repertoire of peripheral blood B memory cells and that epitope spreading appears to occur through the sequential recruitment of nonclonally related autoreactive B cell clones. (uthscsa.edu)

Here, we describe, for the first time, the design and use of peptide arrays covering all human proteins for the analysis of antibody specificity, based on parallel in situ photolithic synthesis of a total of 2.1 million overlapping peptides. (mcponline.org)
Epitope mapping was carried out using microarrays that contained synthesized overlapping peptides covering the full sequence of VlsE from B. burgdorferi B31. (prohealth.com)
With the use of overlapping peptides covering the predicted amino acid sequences of four FhGST cDNAs, we have defined the linear epitopes recognized by polyclonal antibody from sheep vaccinated with FhGST. (jimmunol.org)
These overlapping peptides are assayed for binding reactivity, and the sequence common to the reactive peptides, the consensus sequence, is the epitope ( 8 ). (asm.org)
The ADCC epitopes were mapped using the matrix of overlapping peptides. (frontiersin.org)

Epitope mapping is a powerful tool in analysing the surface topography of an antigen. (innovations-report.com)
The binding of an antibody to the antigen defines a specific binding site or epitope which sterically interferes with the binding of another antibody which has the same or a closely located binding site. (innovations-report.com)
A model study of the use of monoclonal antibodies in capture enzyme immunoassays for antigen quantification exploiting the epitope map of tick-born. (nih.gov)
Hence, we aimed to compare the binding kinetics of a panel of monoclonal antibodies and their relative binding sites to flagellin antigen using a surface plasmon resonance biosensor. (mdpi.com)
Here, we describe a high-resolution method for mapping epitopes of antibodies based on bacterial surface expression of antigen fragments followed by antibody-based flow cytometric sorting. (diva-portal.org)
Epitopes are determined by DNA sequencing of the sorted antibody-binding cells followed by sequence alignment back to the antigen sequence. (diva-portal.org)
Identification and mapping of antibody epitopes on a three-dimensional model of the antigen. (jimmunol.org)
Because the corresponding sequence in CYP3A5 (L N D K QKH) and CYP3A7 (L KE TQKH) differs from CYP3A4 by only two amino acids, six peptides with either one or two amino acid changes were used to determine which amino acid is essential for antibody-antigen interaction. (aspetjournals.org)
In the SPOTs method, antibody-reactive epitopes are mapped on an antigen with known amino acid sequence. (asm.org)
Antigen peptides represent specific epitopes for stimulation of T cells in T cell assays such as ELISPOT. (jpt.com)
Mapping studies with the E1 antigen using an additional five monoclonal antibodies demonstrated that the two strongest inhibitory monoclonal antibodies (18A9 and 21C3) shared the same binding region on E1, whereas the third strongest inhibitor (15A9) displayed an epitope region that overlapped the previous two on the E1 subunit. (researchwithnj.com)
The detection of serum IgG anti-p53 antibodies provides indirect evidence that the immune response to p53 is antigen driven and involves T-helper cells. (aacrjournals.org)
Identifiseringen av en antigen epitop av immunsystemet åpner for forståelsen av den beskyttende mekanisme av nøytraliserende antistoffer som kan fremme utvikling av vaksiner og peptid narkotika. (jove.com)
With a high affinity (K Dapp = 1.3 × 10 -8 M) and no cross-reactivity to either sialyl-Tn epitope or blood group A antigens, scFv G2-D11 is an excellent candidate for a well-defined anti-Tn-antigen reagent. (up.pt)
The observed staining was localized to the cytoplasm and in some cases to the membrane, whereas the surrounding tissue was completely negative demonstrating the usefulness of the novel Tn-antigen binding antibody. (up.pt)
However, it remains to be seen whether high-titered and cross-reactive neutralizing antibodies will be produced by active immunization with a novel viral antigen. (asm.org)
In this study, we attempted to develop a sequential immunization strategy that has proven to be effective at eliciting neutralization antibodies to primary HIV-1 by targeting the HIV-1 Env V3 neutralization epitope site as a model antigen. (asm.org)

These findings provide useful information and suggest a possible strategy for the development and design of an improved abrin-based vaccine and therapeutic antibodies. (jpt.com)
This antibody could be used as a reagent for functional studies of p53, or it may be a candidate for use as an anticancer idiotypic vaccine. (aacrjournals.org)
Fc-mediated functions of Env-specific antibodies, such as antibody-dependent cell cytotoxicity (ADCC), correlated with protection against HIV infection in an RV 144 phase III HIV vaccine trial ( 1 ). (frontiersin.org)
Hence, screening for the identification of the topographical repertoire of B-cell epitopes that elicit cross-protective immune response seems essential in the engineering of a superior PspA-based vaccine. (frontiersin.org)
Herein, we revisit epitope identification in PspA and the utility of hybridoma technology in directing the identification of protective epitope regions of PspA that can be used in vaccine research. (frontiersin.org)
Nevertheless, the identification of three novel antibody specificities within the MPER supports its further study as a promising target for vaccine design. (pubmedcentralcanada.ca)
The induction of broadly neutralizing antibodies has been one of the most pursued outcomes in the development of a preventive vaccine against human immunodeficiency virus type 1 (HIV-1). (pubmedcentralcanada.ca)
The MPER has attracted considerable attention as a potential target for vaccine-induced broadly neutralizing antibodies ( 20 , 23 , 24 ). (pubmedcentralcanada.ca)
Kirkley J.E., Naylor P.H., Marciani D.J., Kensil C.R., Newman M., Goldstein A.L. (1992) QS-21 Augments the Antibody Response to a Synthetic Peptide Vaccine Compared to Alum. (springer.com)
These results demonstrate that sequential immunization with B-cell epitope peptides may contribute to a humoral immune-based HIV vaccine strategy. (asm.org)
Indeed, they help lay the groundwork for the development of HIV-1 vaccine strategies that use sequential immunization with biologically relevant peptides to overcome difficulties associated with otherwise poorly immunogenic epitopes. (asm.org)
We previously reported that the A β peptide vaccine including the T-cell epitope of diphtheria-tetanus combined toxoid (DT) induced anti-A β antibodies, and the prior immunization with conventional DT vaccine enhanced the immunogenicity of the peptide. (hindawi.com)
The peptide vaccine induced anti-A β antibodies in cynomolgus monkeys and guinea pigs without chemical adjuvants, and excessive immune responses were not observed. (hindawi.com)
The peptide vaccine could induce a similar binding profile of anti-A β antibodies in cynomolgus monkeys and guinea pigs. (hindawi.com)
For example, the vaccine against hepatitis B does not elicit an antibody response in approximately 10% of vaccines, and the percentage of nonresponders is substantially higher in immunocompromised persons ( 1 ). (cdc.gov)

In addition to the previously characterized IR6 region in the variable domain, specific sequences in the N- and C-terminal invariable domains of VlsE were found to be major B cell epitopes in affected patients. (prohealth.com)
A comparison of epitopes on FhGST with the B cell epitopes on Sm28, a 28-kDa GST from Schistosoma mansoni, has found few similarities. (jimmunol.org)
We evaluated the amino acid sequence of β-lactamase from Enterobacter cloacae for the presence of human T-cell epitopes using a cell-based proliferation assay using samples from 65 community donors. (aacrjournals.org)
β-Lactamase was found to contain four CD4 + T-cell epitopes. (aacrjournals.org)
The linear B-cell epitopes within the VlsE IR6 peptide were previously mapped using sera from experimentally infected monkeys, from mice, and from humans clinically diagnosed with Lyme disease using an overlapping peptide technique. (asm.org)
An approach to the identification of T cell epitopes in the genomic era: application to Francisella tularensis. (rochester.edu)

Since linear epitopes of PspA were not protective, larger overlapping fragments containing 100 amino acids of PspA of strain Rx1 were constructed (fragments 1 to 7, numbered from the N terminus) to permit the mapping of antibodies with conformational epitopes not represented in the peptide arrays. (asm.org)
Antibodies from mice immunized with fragments 1, 2, 4, and 5 were capable of binding onto the surface of pneumococci and mediating protection against a lethal challenge. (asm.org)
The fact that immunization of mice with 100-amino-acid fragments located at the more conserved N-terminal region of PspA (fragments 1 and 2) induced protection against a pneumococcal challenge indicates that the induction of antibodies against conformational epitopes present at this region may be important in strategies for inducing broad protection against pneumococci. (asm.org)
Monoclonal antibodies raised in mice were used to immunoscreen random ADAMTS13 peptide fragments expressed by Lambda gt 11 bacteriophage. (kzoo.edu)
The ADAMTS13 fragments to which each monoclonal antibody bound were identified by sequencing the corresponding Lambda gt 11 cDNA, and the minimal linear peptide sequence necessary for antibody recognition and binding was determined. (kzoo.edu)
Our human anti-CEA IgG2κ is a promising candidate for radioimmunotherapy in intact form, as F(ab') 2 fragments, or as a bispecific antibody. (biomedcentral.com)

By selecting human antibody gene diversity libraries directly on the surface of prostate cancer cells, we have taken a functional approach to identifying fully human, tumor-specific monoclonal antibodies without prior knowledge of their target antigens. (aacrjournals.org)
Selection conditions have been optimized to favor tumor-specific antibody binding and internalization. (aacrjournals.org)
These tumor-specific internalizing antibodies are likely to be useful for targeted therapeutics either alone or in combination with effector molecules. (aacrjournals.org)
The antigens they bind constitute a tumor-specific internalizing epitope space that is likely to play a significant role in cancer cell homeostasis. (aacrjournals.org)
Targeting components of this epitope space may facilitate development of immunotherapeutic and small molecule-based strategies as well as the use of other therapeutic agents that rely upon delivery to the interior of the tumor cell. (aacrjournals.org)
Peptide microarrays that display overlapping peptide scans through antigens from infectious organisms or tumor associated antigens for antibody or serum profiling. (jpt.com)
As a first step in understanding the anti-tumor activity of ATN-658, we set out to identify the epitope on uPAR to which ATN-658 binds. (plos.org)
These studies reveal a new functional epitope on uPAR involved in tumor progression and demonstrate a previously unrecognized strategy for the therapeutic targeting of uPAR. (plos.org)
Human antibodies have been isolated against a number of tumor antigens, including p53 (18) , and these human anti-p53 antibodies were shown to bind with high affinity to an epitope between residues 27 and 44 of the NH 2 terminus of p53. (aacrjournals.org)
Antibody-directed enzyme prodrug therapy (ADEPT) delivers chemotherapeutic agents in high concentration to tumor tissue while minimizing systemic drug exposure. (aacrjournals.org)
a ) an antibody fragment that binds selectively to tumor tissue and ( b ) an enzyme domain that catalyzes the conversion of a nontoxic prodrug to an active drug at the tumor site. (aacrjournals.org)
A broadly applicable approach to T cell epitope identification: application to improving tumor associated epitopes and identifying epitopes in complex pathogens. (rochester.edu)
Here we report the identification of several MET monoclonal antibodies (mAb) that block MET-dependent signaling and tumor growth. (aacrjournals.org)

We produced five new anti-HER2 monoclonal antibodies, all directed against conformational epitope or epitopes restricted to the native form of the extracellular domain. (biomedcentral.com)

Antibodies are of importance for the field of proteomics, both as reagents for imaging cells, tissues, and organs and as capturing agents for affinity enrichment in mass-spectrometry-based techniques. (mcponline.org)
Knowledge about an antibody's linear epitopes is also useful in, for instance, developing assays involving the capture of peptides obtained from trypsin cleavage of samples prior to mass spectrometry analysis. (mcponline.org)
A large number of methods have therefore been developed to determine the epitopes of antibodies, including mass spectrometry ( 9 ), solid phase libraries ( 10 , 11 ), and different display systems ( 12 ⇓ - 14 ) such as bacterial display ( 15 ) and phage display ( 16 ). (mcponline.org)

The unique property of specific high-affinity binding to more or less any target of interest has made antibodies tremendously useful in numerous applications. (diva-portal.org)
Complementary binding peptides are then assayed by affinity binding of the antibody. (asm.org)
These strong antibody responses may be caused by affinity maturation as a result of repeated exposure to the virus or by the fact that dromedaries-apart from conventional antibodies-have relatively unique, heavy chain-only antibodies (HCAbs). (sciencemag.org)
We observed a wide range of anti-HCV potencies that were independent of their epitope grouping, but associated with their relative affinity for cell-surface expressed CD81. (ox.ac.uk)
KD-247 bound with high affinity to the "PGR" motif within the HIV-1 Env V3 tip region, and, among the established reference antibodies, it most effectively neutralized primary HIV-1 field isolates possessing the matching neutralization sequence motif, suggesting its promise for clinical applications involving passive immunizations. (asm.org)
While in theory, high-affinity antibodies that recognize the relatively conserved GPGR epitope could potentially neutralize many strains of HIV-1 clade B, in practice, such antibodies in sera from HIV-infected individuals show little neutralization activity in vitro ( 2 ), suggesting that the immunogenicity of the GPGR sequence is similarly low. (asm.org)

The results from this study support the notion of PCV2 modulation of immunity, including antibody responses that may represent a precursor for disease. (k-state.edu)

However, it is known that some individuals develop broadly cross-neutralizing antibodies during natural HIV-1 infection ( 5 , 6 , 18 , 25 , 26 ). (pubmedcentralcanada.ca)
In some cases, broadly cross-neutralizing antibodies have been mapped to the CD4 binding site, the coreceptor binding site (CD4i), and other undefined epitopes within gp120. (pubmedcentralcanada.ca)
With regard to the role of neutralizing antibody responses in HIV-1 infection, broadly reactive neutralizing antibodies such as 2G12 ( 54 ), 2F5 ( 45 ), and 4E10 ( 5 ) have been proven to suppress immune deficiency virus infection in macaques ( 14 , 38 ) and humans ( 62 ). (asm.org)
A sound rationale exists for antibody targeting of the MET receptor tyrosine kinase, but therapeutic agents that can broadly block HGF ligand binding and exon 14-mutated or amplified MET to induce receptor degradation have yet to be reported. (aacrjournals.org)

Although some studies suggest that antibodies against ZIKV NS1 are protective, their activity during congenital infection is unknown. (nature.com)
Anti-NS1 antibodies provide an alternative strategy for protection against congenital ZIKV infection without causing ADE. (nature.com)
These data demonstrate the existence of MPER-specific cross-neutralizing antibodies in plasma, although the ability to elicit such potent antiviral antibodies during natural infection appears to be rare. (pubmedcentralcanada.ca)
Therapeutic antibody cocktails, however, have shown efficacy against otherwise lethal Ebola virus infection and show significant promise for eventual human use. (pnas.org)
Monoclonal antibody (mAb) cocktails are particularly attractive candidates due to their proven postexposure efficacy in nonhuman primate models of EBOV infection. (pnas.org)
However, the specific antibodies that confer protective immunity against human immunodeficiency virus type 1 (HIV-1) infection are not well known, since most primary strains of HIV-1 are relatively resistant to neutralization ( 40 , 47 ). (asm.org)

This article proposes a biological defense initiative based on developing, producing, and stockpiling specific antibody reagents that can be used to protect the population against biological warfare threats. (cdc.gov)

In spite of the substantial effort invested in the design of an immunogen capable of inducing such antibodies, little success has been achieved. (pubmedcentralcanada.ca)
3 The study's hypothesis was that these two adjuvants function by different means to increase antibody titer to a synthetic peptide immunogen. (springer.com)

Accordingly, we report here the isolation of 45 BG505 nAbs with multiple specificities from immunized and infected rhesus macaques.Notably, all of the autologous nAbs bind in close proximity to known bnAb epitopes and might therefore sterically hinder elicitation of bnAbs. (ssrn.com)

The identification of an 11-1F4-related mimotope should facilitate development of pan-light chain fibril-reactive antibodies that could be used in the diagnosis and treatment of patients with AL amyloidosis. (nih.gov)
These patents are US# 8,101,726 "Ligands binding the complex of urokinase type plasminogen activator (uPA) and its receptor (uPAR) that inhibit downstream uPAR interactions: identification and use in diagnosis or therapy" (Parry and Mazar) and US# 8,105,602 "Urokinase type plasminogen activator receptor epitope, monoclonal antibodies derived therefrom and methods of use thereof" (Parry and Mazar). (plos.org)
Identification of a dominant CD4 T cell epitope in the membrane lipoprotein Tul4 from Francisella tularensis LVS. (rochester.edu)
Antibodies to CD99 are used in diagnostic immunohistochemistry to distinguish Ewing's sarcoma from other tumours of similar histological appearance, as well as for the identification of thymic tumours, and of spindle cell tumours, such as synovial sarcoma, haemangiopericytoma, and meningioma. (wikipedia.org)

For epitope mapping, pairwise comparisons were completed to determine the binding inhibition of each paired combination of monoclonal antibodies. (mdpi.com)
An antipeptide antibody has been produced that recognizes CYP3A4 and exhibits greater than 90-95% inhibition on CYP3A4-mediated reactions [Wang RW and Lu AYH (1997) Drug Metab Dispos 25:762- (aspetjournals.org)
This conclusion was based on the reversal of antibody inhibition of testosterone 6β-hydroxylation when peptides with overlapping sequence in this region were preincubated with the antibody. (aspetjournals.org)
Incubation of CMY-2 β-lactamase with a 10-fold molar excess of anti-CMY-2 antibody for 60 min resulted in greater than 80% inhibition of nitrocefin hydrolysis. (asm.org)
To further explore the role of antibodies in ADAMTS13 inhibition, this study examined the minimal peptide sequence recognized by a monoclonal antibody. (kzoo.edu)
Of the 10 monoclonal antibodies that showed the greatest inhibition of PDHc, 4 were elicited by PDHc and 6 by E1. (researchwithnj.com)
The three monoclonal antibodies that demonstrated the strongest inhibition appeared to bind the same region on the PDHc. (researchwithnj.com)
We elucidated that the blocking activity of these antibodies is mediated through 2 mechanisms involving inhibition of HGF binding and induction of receptor degradation. (aacrjournals.org)

CD4-induced (CD4i) antibodies bind gp120 more efficiently after CD4 binding and block the interaction with the chemokine receptor. (nih.gov)
90 monoclonal antibodies that specifically bind and enter prostate cancer cells, with little or no binding to control cells. (aacrjournals.org)
In particular, the MET mAb KTN0073 and KTN0074 bind the Sema/PSI domain, at overlapping but distinct epitopes, preventing HGF interaction with MET and triggering receptor ubiquitination and degradation. (aacrjournals.org)
In contrast to antibodies used in cancer therapeutics, which aim to stimulate an immune attack, therapeutic antibodies for inflammatory and autoimmune diseases are designed to do the opposite: neutralizing antibodies bind to pro-inflammatory factors and prevent them from exacerbating the immune response. (genscript.com)

We have used the SPOT peptide synthesis method to map the linear epitopes recognized by anti-SHV-1 and anti-CMY-2 polyclonal antibodies to their respective antigens. (asm.org)
Epitopes capable of inducing antibodies with broad cross-reactivities should thus be the preferred antigens. (asm.org)

The use of recombinant human antibody techniques has recently provided insight into the molecular basis of the humoral immune response to many diseases, including cancer. (aacrjournals.org)
Readily reproducible protocols for lead generation range from the cloning of human immunoglobulin genes to the generation of human recombinant antibodies by humanization approaches, molecular display technologies, and transgenic animals. (springer.com)

There was no correlation between an antibody response to linear peptide epitopes and the level of protection induced in sheep by vaccination with FhGST. (jimmunol.org)

The aim of this study was to identify individuals with serum reactivity against the central and COOH-terminal domains of p53 and to isolate and characterize these recombinant human antibodies. (aacrjournals.org)

Antibody phage display libraries were constructed from four immunoreactive individuals, and the libraries were panned against the central and COOH-terminal domains of p53. (aacrjournals.org)

Aiming to identify the immunodominant epitopes of abrin, several neutralizing antiabrin polyclonal antibodies were screened using a set of 15-mer peptides spanning the amino acid sequence of either the A or B subunits of abrin. (jpt.com)

To gain a comprehensive picture of the antibody response induced upon 4CMenB vaccination and to obtain relevant translational information directly from human studies, we have isolated a panel of human monoclonal antibodies from adult vaccinees. (omicsdi.org)
Mice transgenic for human immunoglobulin genes were immunized with CfaE to generate a panel of human monoclonal IgG1 antibodies (HuMAbs). (asm.org)

Toluene diisocyanate (TDI)-specific monoclonal antibodies: production and epitope mapping. (cdc.gov)
Dominant and minor epitopes were found to be present on all four of the sequences although some epitopes were shown to be specific to particular FhGST. (jimmunol.org)
A modified evolutionary trace (ET) analysis called reverse ET (RET) was used to predict the type-specific B-cell neutralizing epitopes. (antibody-antibodies.com)
It was found that the type-specific neutralizing epitopes of HAdV3 are located at the top surface of hexon tower regions (residue numbers: 135-146, 169-178, 237-251, 262-272, 420-434). (antibody-antibodies.com)
An antibody fragment (1159.8) that was specific for the whole molecule as well as the central domain was isolated from one of the libraries. (aacrjournals.org)
HIV-specific antibody-dependent cell cytotoxicity (ADCC) is likely to be important in governing protection from human immunodeficiency virus (HIV) and slowing disease progression. (frontiersin.org)
In order to establish if these antibodies were directly responsible for the observed neutralization breadth, we used MPER-coated magnetic beads to deplete plasmas of these specific antibodies. (pubmedcentralcanada.ca)
Once the amino acids detected by the antibody are determined, the surrounding amino acids can be exchanged to reveal the influence of the environment on the detection of a specific epitope. (qiagen.com)
The specific epitope has not been mapped. (fishersci.com)
Passive antibody therapy has substantial advantages over antimicrobial agents and other measures for postexposure prophylaxis, including low toxicity and high specific activity. (cdc.gov)
This article reviews the activity of humoral immunity against several biological agents, discusses the advantages and disadvantages of an antibody-based defense strategy ( Table ), and proposes stockpiling specific antibodies for use in the event of biological attacks. (cdc.gov)

This has allowed analysis of on- and off-target binding of both monoclonal and polyclonal antibodies, complemented with precise mapping of epitopes based on full amino acid substitution scans. (mcponline.org)
Single amino acid deletions identified DMVLNETLRL as the minimum amino acid sequence required for antibody binding. (aspetjournals.org)
Dr. Bloom has developed a method to map and quantify how all possible amino-acid mutations to Env affect antibody neutralization. (fhcrc.org)

Epitope mapping is widely applied as an efficient tool for discovering the antigenic moieties of toxins, thus providing invaluable information needed for the development of vaccines and therapies. (jpt.com)
Information derived from these mapping methods can inform which epitopes to best target for development of antibodies and vaccines to HIV. (fhcrc.org)
Alternatives to traditional antiretroviral therapy are a highly active area of research, including vaccines, synthetic peptides, and antibodies. (fhcrc.org)

Antibodies directed to different epitopes on the extracellular domain of HER2 are promising candidates for replacement or combinatorial therapy. (biomedcentral.com)

With over 20 monoclonal-antibody-based drugs now on the market and over 100 in clinical trials, the field of antibody therapeutics has become a central component of the pharmaceutical industry ( 7 ). (mcponline.org)
Following the discovery of ERBB2 gene amplifications in breast cancer, the HER2 receptor became an attractive target for monoclonal antibody-based therapies. (biomedcentral.com)

Here we have used recombinant alpha-synucleins and peptide competition to show that the monoclonal anti-alpha-synuclein antibody LB509 recognizes amino acids 115-122 of human alpha-synuclein. (nih.gov)

AMG 102 is a fully human monoclonal antibody that selectively targets and neutralizes hepatocyte growth factor/scatter factor (HGF/SF). (aacrjournals.org)

Efforts to select protective antibodies to P. aeruginosa and other pathogens have been mostly target-centric, focusing on bacterial surface features or virulence factors correlated with disease. (rupress.org)

Examples of CD4i antibodies are limited, and the prototypes of the CD4i antibodies exhibit only weak neutralizing activity against primary, clinical HIV-1 isolates. (nih.gov)
In the absence of erythema migrans, the basis for diagnosis of Lyme disease is the demonstration of an antibody response against Borrelia burgdorferi in an appropriate clinical setting. (asm.org)
These findings and emerging clinical data support the discovery and development of MET-targeting antibodies and TKIs as therapeutic options for patients. (aacrjournals.org)

Of the five monoclonal antibodies, only two interfered with the binding of each other. (mdpi.com)

A detailed analysis of the recognition sites that are located in an area analogous to the omega loop of class A β-lactamases (V211 to V223) showed that the amino acids Q215 to E219 are important in antibody binding. (asm.org)
In the present study we wanted to discern whether SHV-1 variants are recognized as well as SHV-1 by the anti-SHV-1 antibody or whether SHV recognition due to mutations would be altered. (asm.org)
Alanine scanning identified Y-173, F-174, Q-175 and K-179 as important for antibody recognition. (k-state.edu)
No known function has previously been attributed to this epitope Structural insights into epitope recognition were obtained from structural studies of the Fab fragment of ATN-658 bound to uPAR. (plos.org)

Of these, seventeen monoclonal antibodies bound to PA polypeptide spanning amino acids 101-400 and three bound to that of amino acids 518-600, while the other two did not react with any PA polypeptides tested with the exception of full-length PA. (deepdyve.com)
another (II) bound to sites that overlapped with epitopes in either region I or III. (elsevier.com)

Anatomy7

Organisms8

Diseases1

Chemicals and Drugs55

Analytical, Diagnostic and Therapeutic Techniques and Equipment25

Phenomena and Processes24

Information Science3

Health Care1

Bacterial display

CD99

FLOT1

FLOT2

Immunomics

Immunofluorescence

Rashna Bhandari

CYP2C18

Rabies virus

Peptide microarray

Dihydrolipoyl transacetylase

Simone Warner

Antonio Lanzavecchia

Eva Engvall

Tamas Bartfai

MYOM1

Ekkehard Bautz

Epitope

Chlamydia muridarum

Epitope mapping

Conformational epitope

Matuzumab

Epitope binning

SNRPB

Fibroblast activation protein, alpha

B3GAT1

B3GAT2

Dengue virus

Computational immunology

CD200 - Википедия

Single cell sequencing

Меланотрансферрин - Википедия

Interleukin 8 receptor, alpha

Latex allergy

Xenotransplantation

ಮಲೇರಿಯಾ - ವಿಕಿಪೀಡಿಯ

Flow cytometry

Systemic lupus erythematosus

Natural killer cell

T helper cell

권성훈 - 위키백과, 우리 모두의 백과사전

Hsp90

Radioallergosorbent test

Immunoprecipitation

Immunosignature

Antigenic variation

Envelope glycoprotein GP120

Latex fixation test

Neuroscience Information Framework

MHC class I

Immunohistochemistry

Collagen, type XVIII, alpha 1

CD97

Protein microarray

மலேரியா - தமிழ் விக்கிப்பீடியா

Phage display

Reverse phase protein lysate microarray

ACAD10

NIOSHTIC-2 Publications Search - 20035265 - Toluene diisocyanate (TDI)-specific monoclonal antibodies: production and epitope...

Pair-wise Epitope mapping of monoclonal antibodies - Innovations Report

Proteome-wide Epitope Mapping of Antibodies Using Ultra-dense Peptide Arrays | Molecular & Cellular Proteomics

Proteome-wide Epitope Mapping of Antibodies Using Ultra-dense Peptide Arrays | Molecular & Cellular Proteomics

Characterization and epitope mapping of Dengue virus type 1 specific monoclonal antibodies. | Sigma-Aldrich

Epitope mapping of LB509, a monoclonal antibody directed against human alpha-synuclein

A model study of the use of monoclonal antibodies in capture enzyme immunoassays for antigen quantification exploiting the...

Mapping Neutralizing Antibody Epitope Specificities to an HIV Env Trimer in Immunized and in Infected Rhesus Macaques by...

Epitope mapping and characterization of a novel CD4-induced human monoclonal antibody capable of neutralizing primary HIV-1...

Antibodies | Free Full-Text | Kinetic Analysis and Epitope Mapping of Monoclonal Antibodies to Salmonella Typhimurium Flagellin...

Epitope mapping of antibodies to VlsE protein of Borrelia burgdorferi in post-Lyme disease syndrome. - Prohealth

Epitope mapping studies with human anti-cytochrome P450 3A antibodies. | Molecular Pharmacology

Epitope mapping of monoclonal and polyclonal antibodies using bacterial cell surface display

Mapping Tumor Epitope Space by Direct Selection of Single-Chain Fv Antibody Libraries on Prostate Cancer Cells | Cancer Research

Epitope mapping and characterization of a novel CD4 induced human monoclonal antibody capable of neutralizing primary HIV 1...

Vaccination of sheep against Fasciola hepatica with glutathione S-transferase. Identification and mapping of antibody epitopes...

Inhibitory Anti-CYP3A4 Peptide Antibody: Mapping of Inhibitory Epitope and Specificity Toward Other CYP3A Isoforms | Drug...

Epitope mapping of the influenza A virus RNA polymerase PA using monoclonal antibodies, Archives of Virology | 10.1007...

Mapping the epitope of a functional P-selectin monoclonal antibody (LYP20) to a short complement-like repeat (SCR 4) domain:...

Antibody Mapping of the Linear Epitopes of CMY-2 and SHV-1 β-Lactamases | Antimicrobial Agents and Chemotherapy

Mapping of Epitopes Recognized by Antibodies Induced by Immunization of Mice with PspA and PspC | Clinical and Vaccine...

Proteomic Mapping and Molecular Modelling of Avian H5N1 Influenza Antigen Epitopes Targeted by Human Antibodies - Research...