Luciferases, Bacterial: Luciferases from BACTERIA such as PHOTOBACTERIUM; VIBRIO; and PHOTORHABDUS.Biotin: A water-soluble, enzyme co-factor present in minute amounts in every living cell. It occurs mainly bound to proteins or polypeptides and is abundant in liver, kidney, pancreas, yeast, and milk.Vibrio: A genus of VIBRIONACEAE, made up of short, slightly curved, motile, gram-negative rods. Various species produce cholera and other gastrointestinal disorders as well as abortion in sheep and cattle.Flavin Mononucleotide: A coenzyme for a number of oxidative enzymes including NADH DEHYDROGENASE. It is the principal form in which RIBOFLAVIN is found in cells and tissues.Luciferases: Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms have evolved differently so have different structures and substrates.Sodium Azide: A cytochrome oxidase inhibitor which is a nitridizing agent and an inhibitor of terminal oxidation. (From Merck Index, 12th ed)Luminescent Measurements: Techniques used for determining the values of photometric parameters of light resulting from LUMINESCENCE.Photobacterium: A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that are common in the marine environment and on the surfaces and in the intestinal contents of marine animals. Some species are bioluminescent and are found as symbionts in specialized luminous organs of fish.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Vibrionaceae: A family of gram-negative bacteria whose members predominate in the bacterial flora of PLANKTON; FISHES; and SEAWATER. Some members are important pathogens for humans and animals.Cell Surface Display Techniques: Techniques utilizing cells that express RECOMBINANT FUSION PROTEINS engineered to translocate through the CELL MEMBRANE and remain attached to the outside of the cell.Epitope Mapping: Methods used for studying the interactions of antibodies with specific regions of protein antigens. Important applications of epitope mapping are found within the area of immunochemistry.Epitopes: Sites on an antigen that interact with specific antibodies.Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Antibody Specificity: The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Antibodies, Viral: Immunoglobulins produced in response to VIRAL ANTIGENS.Antibodies, Bacterial: Immunoglobulins produced in a response to BACTERIAL ANTIGENS.Bacterial Vaccines: Suspensions of attenuated or killed bacteria administered for the prevention or treatment of infectious bacterial disease.Typhoid-Paratyphoid Vaccines: Vaccines used to prevent TYPHOID FEVER and/or PARATYPHOID FEVER which are caused by various species of SALMONELLA. Attenuated, subunit, and inactivated forms of the vaccines exist.HIV-1: The type species of LENTIVIRUS and the etiologic agent of AIDS. It is characterized by its cytopathic effect and affinity for the T4-lymphocyte.Immunoconjugates: Combinations of diagnostic or therapeutic substances linked with specific immune substances such as IMMUNOGLOBULINS; MONOCLONAL ANTIBODIES; or ANTIGENS. Often the diagnostic or therapeutic substance is a radionuclide. These conjugates are useful tools for specific targeting of DRUGS and RADIOISOTOPES in the CHEMOTHERAPY and RADIOIMMUNOTHERAPY of certain cancers.HIV Infections: Includes the spectrum of human immunodeficiency virus infections that range from asymptomatic seropositivity, thru AIDS-related complex (ARC), to acquired immunodeficiency syndrome (AIDS).Vaccines, Attenuated: Live vaccines prepared from microorganisms which have undergone physical adaptation (e.g., by radiation or temperature conditioning) or serial passage in laboratory animal hosts or infected tissue/cell cultures, in order to produce avirulent mutant strains capable of inducing protective immunity.Simian immunodeficiency virus: Species of the genus LENTIVIRUS, subgenus primate immunodeficiency viruses (IMMUNODEFICIENCY VIRUSES, PRIMATE), that induces acquired immunodeficiency syndrome in monkeys and apes (SAIDS). The genetic organization of SIV is virtually identical to HIV.Immunotoxins: Semisynthetic conjugates of various toxic molecules, including RADIOACTIVE ISOTOPES and bacterial or plant toxins, with specific immune substances such as IMMUNOGLOBULINS; MONOCLONAL ANTIBODIES; and ANTIGENS. The antitumor or antiviral immune substance carries the toxin to the tumor or infected cell where the toxin exerts its poisonous effect.HIV: Human immunodeficiency virus. A non-taxonomic and historical term referring to any of two species, specifically HIV-1 and/or HIV-2. Prior to 1986, this was called human T-lymphotropic virus type III/lymphadenopathy-associated virus (HTLV-III/LAV). From 1986-1990, it was an official species called HIV. Since 1991, HIV was no longer considered an official species name; the two species were designated HIV-1 and HIV-2.Acquired Immunodeficiency Syndrome: An acquired defect of cellular immunity associated with infection by the human immunodeficiency virus (HIV), a CD4-positive T-lymphocyte count under 200 cells/microliter or less than 14% of total lymphocytes, and increased susceptibility to opportunistic infections and malignant neoplasms. Clinical manifestations also include emaciation (wasting) and dementia. These elements reflect criteria for AIDS as defined by the CDC in 1993.Fimbriae, Bacterial: Thin, hairlike appendages, 1 to 20 microns in length and often occurring in large numbers, present on the cells of gram-negative bacteria, particularly Enterobacteriaceae and Neisseria. Unlike flagella, they do not possess motility, but being protein (pilin) in nature, they possess antigenic and hemagglutinating properties. They are of medical importance because some fimbriae mediate the attachment of bacteria to cells via adhesins (ADHESINS, BACTERIAL). Bacterial fimbriae refer to common pili, to be distinguished from the preferred use of "pili", which is confined to sex pili (PILI, SEX).Fimbriae Proteins: Proteins that are structural components of bacterial fimbriae (FIMBRIAE, BACTERIAL) or sex pili (PILI, SEX).Enterotoxigenic Escherichia coli: Strains of ESCHERICHIA COLI that produce or contain at least one member of either heat-labile or heat-stable ENTEROTOXINS. The organisms colonize the mucosal surface of the small intestine and elaborate their enterotoxins causing DIARRHEA. They are mainly associated with tropical and developing countries and affect susceptible travelers to those places.Bacterial Proteins: Proteins found in any species of bacterium.Bacterial Adhesion: Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Adhesins, Escherichia coli: Thin, filamentous protein structures, including proteinaceous capsular antigens (fimbrial antigens), that mediate adhesion of E. coli to surfaces and play a role in pathogenesis. They have a high affinity for various epithelial cells.Escherichia coli Infections: Infections with bacteria of the species ESCHERICHIA COLI.Escherichia coli Proteins: Proteins obtained from ESCHERICHIA COLI.Enterotoxins: Substances that are toxic to the intestinal tract causing vomiting, diarrhea, etc.; most common enterotoxins are produced by bacteria.Polysaccharides, Bacterial: Polysaccharides found in bacteria and in capsules thereof.PolysaccharidesBacterial Capsules: An envelope of loose gel surrounding a bacterial cell which is associated with the virulence of pathogenic bacteria. Some capsules have a well-defined border, whereas others form a slime layer that trails off into the medium. Most capsules consist of relatively simple polysaccharides but there are some bacteria whose capsules are made of polypeptides.IgG Deficiency: A dysgammaglobulinemia characterized by a deficiency of IMMUNOGLOBULIN G.Fungal Polysaccharides: Cell wall components constituting a polysaccharide core found in fungi. They may act as antigens or structural substrates.Complementarity Determining Regions: Three regions (CDR1; CDR2 and CDR3) of amino acid sequence in the IMMUNOGLOBULIN VARIABLE REGION that are highly divergent. Together the CDRs from the light and heavy immunoglobulin chains form a surface that is complementary to the antigen. These regions are also present in other members of the immunoglobulin superfamily, for example, T-cell receptors (RECEPTORS, ANTIGEN, T-CELL).Carbohydrate Sequence: The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.Streptococcus pneumoniae: A gram-positive organism found in the upper respiratory tract, inflammatory exudates, and various body fluids of normal and/or diseased humans and, rarely, domestic animals.Immunoglobulin G: The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.Endocarditis, Subacute Bacterial: ENDOCARDIUM infection that is usually caused by STREPTOCOCCUS. Subacute infective endocarditis evolves over weeks and months with modest toxicity and rare metastatic infection.Borrelia burgdorferi: A specific species of bacteria, part of the BORRELIA BURGDORFERI GROUP, whose common name is Lyme disease spirochete.Endocarditis, Bacterial: Inflammation of the ENDOCARDIUM caused by BACTERIA that entered the bloodstream. The strains of bacteria vary with predisposing factors, such as CONGENITAL HEART DEFECTS; HEART VALVE DISEASES; HEART VALVE PROSTHESIS IMPLANTATION; or intravenous drug use.Borrelia burgdorferi Group: Gram-negative helical bacteria, in the genus BORRELIA, that are the etiologic agents of LYME DISEASE. The group comprises many specific species including Borrelia afzelii, Borellia garinii, and BORRELIA BURGDORFERI proper. These spirochetes are generally transmitted by several species of ixodid ticks.Lyme Disease: An infectious disease caused by a spirochete, BORRELIA BURGDORFERI, which is transmitted chiefly by Ixodes dammini (see IXODES) and pacificus ticks in the United States and Ixodes ricinis (see IXODES) in Europe. It is a disease with early and late cutaneous manifestations plus involvement of the nervous system, heart, eye, and joints in variable combinations. The disease was formerly known as Lyme arthritis and first discovered at Old Lyme, Connecticut.Borrelia Infections: Infections with bacteria of the genus BORRELIA.Streptococcal Infections: Infections with bacteria of the genus STREPTOCOCCUS.Ixodes: The largest genus of TICKS in the family IXODIDAE, containing over 200 species. Many infest humans and other mammals and several are vectors of diseases such as LYME DISEASE, tick-borne encephalitis (ENCEPHALITIS, TICK-BORNE), and KYASANUR FOREST DISEASE.Endocarditis: Inflammation of the inner lining of the heart (ENDOCARDIUM), the continuous membrane lining the four chambers and HEART VALVES. It is often caused by microorganisms including bacteria, viruses, fungi, and rickettsiae. Left untreated, endocarditis can damage heart valves and become life-threatening.Streptococcus: A genus of gram-positive, coccoid bacteria whose organisms occur in pairs or chains. No endospores are produced. Many species exist as commensals or parasites on man or animals with some being highly pathogenic. A few species are saprophytes and occur in the natural environment.Antibody Formation: The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.Ultraviolet Rays: That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants.Walkers: Walking aids generally having two handgrips and four legs.Carcinoma 256, Walker: A transplantable carcinoma of the rat that originally appeared spontaneously in the mammary gland of a pregnant albino rat, and which now resembles a carcinoma in young transplants and a sarcoma in older transplants. (Stedman, 25th ed)Immunologic Memory: The altered state of immunologic responsiveness resulting from initial contact with antigen, which enables the individual to produce antibodies more rapidly and in greater quantity in response to secondary antigenic stimulus.Diphtheria Toxoid: The formaldehyde-inactivated toxin of Corynebacterium diphtheriae. It is generally used in mixtures with TETANUS TOXOID and PERTUSSIS VACCINE; (DTP); or with tetanus toxoid alone (DT for pediatric use and Td, which contains 5- to 10-fold less diphtheria toxoid, for other use). Diphtheria toxoid is used for the prevention of diphtheria; DIPHTHERIA ANTITOXIN is for treatment.Immunization, Secondary: Any immunization following a primary immunization and involving exposure to the same or a closely related antigen.Dinitrophenols: Organic compounds that contain two nitro groups attached to a phenol.

The indirect hemagglutination test for the detection of antibodies in cattle naturally infected mycoplasmas. (1/11816)

Stable mycoplasma antigens for the indirect hemagglutination test (IHA) were prepared employing glutaraldehyde treated sheep erythrocytes sensitized with Mycoplasma agalactiae subsp. bovis and Mycoplasma bovigenitalium antigens. Employing these antigens mycoplasma antibodies were detected in sera from cattle which had mastitic symptoms due to natural infection with either M. agalactiae subsp. bovis or M. bovigenitalium. A total of 200 cows from four herds were examined at varying intervals for the presence of M. agalactiae subsp. bovis and for the detection of antibody using growth inhibition and IHA tests. Mycoplasmas were isolated from 37 animals. Growth inhibiting antibody was detected from 56 of the 200 animals. In the IHA tests, antibody titer greater than or equal to 1:80 were detected in 148 animals, 76 of these having antibody titers greater than or equal to 1:160, while sera of 116 normal control animals had no growth inhibiting antibody and none had IHA antibody titers greater than 1:40. M. bovigenitalium was isolated from the milk of three of 26 animals in a fifth herd during an outbreak of mastitis. Growth inhibiting antibodies were demonstrated in the sera of ten of the 26 animals. However, the IHA test detected antibody titers of greater than or equal to 1:160 in 13 animals and of 1:80 in one of the 26 animals. To determine the specificity of the IHA tests, M. agalactiae subsp. bovis and M. bovigenitalium antigens were reacted with rabbit hyperimmune typing sera produced against 12 species of bovine mycoplasmatales. Homologous antisera showed IHA antibody titers of 1:1280 and 1:2560 against M. agalactiae subsp. bovis and M. bovigenitalium respectively, whereas heterologous antisera showed IHA antibody titers of less than or equal to 1:20. Also eight type-specific bovine antisera were reacted with M agalactiae subsp. bovis and M. bovigenitalium antigens in homologous and heterologous tests. Homoogous reactions showed IHA antibody titers greater than or equal to 1:320, whereas heterologous reactions showed IHA titers of less than or equal to 1:20. This IHA test promises to be useful for the detection of bovine mycoplasma antibodies in sera from cattle infected with M. agalactiae subsp. bovis or M. bovigenitalium. Thes test is sensitive, reproducible and specific and the technique is relatively simple and rapid. The antigens were stable for at least seven months.  (+info)

Studies on the response of ewes to live chlamydiae adapted to chicken embryos or tissue culture. (2/11816)

Ewes infected before gestation with chicken embryo or tissue culture adapted chlamydial strain B-577 were challenge inoculated with the homologous strain at four to 18 weeks of gestation. The ewes responsed with group specific complement fixing antibody titers of 1:8 to 1:256 by the second week after initial infection. A secondary antibody response in the surviving challenge inoculated ewes occurred at the time of lambing and reached titers of 1:32 to 1:256 by the second week after parturition. Group specific complement fixing antibodies did not appear to play a significant role in resistance to chlamydial infection. Ewes infected with the chicken embryo adapted strain B-577 excreted chlamydiae in their feces 60 days after inoculation. However, chlamydiae were not recovered from feces of ewes infected with the tissue culture adapted strain B-577. Placentas of ewes challenge inoculated by the intravenous route were consistently infected. Chlamydiae were recovered from placentas, some fetuses and lambs. In two instances when challenge inoculation was given by the intramuscular route, infection was detected only by the direct fluorescent antibody method.  (+info)

Experimental production of infectious bovine keratoconjunctivitis: comparison of serological and immunological responses using pili fractions of Moraxella bovis. (3/11816)

The effect of vaccinating cattle and mice on the development of keratoconjunctivitis was studied. Cattle were vaccinated with whole cells, disrupted cells and pili fractions of three strains of Moraxella bovis. Mice were vaccinated with pili fractions of three strains. The resistance of all vaccinated animals was challenged with virulent cultures of M. bovis. In an attempt to correlate the response seen after vaccination and challenge with a pili fraction of M. bovis, vaccinated cattle and mice were grouped on the basis of signs of disease manifested and compared on the basis of serological responses. Serum samples were tested for antibodies by a gel diffusion precipitin test. A greater number of the sera of resistant cattle had antibodies to the homologous pili antigen than those of vaccinated nonresistant cattle. Cattle vaccinated with disrupted cells were not resistant to infectious bovine kerato-conjuctivitis and their sera lacked antibodies against the pili antigens. Vaccinated mice were more resistant to infectious bovine kerato-conjuctivitis and their sera lacked antibodies against the pili antigens. Vaccinated mice were more resistant to challenge exposure by homologous than heterologous cultures. A greater number of the sera of resistant mice had antibodies to pili antigens than nonresistant mice.  (+info)

The effect of route of immunization on the lapine immune response to killed Pasteurella haemolytica and the influence of aerosol challenge with the live organism. (4/11816)

Appearance of anti-Pasteurella haemolytica antibody in the serum and broncho-alveolar washings of rabbits is independent of the route of immunization and is similar in both locations. The most influential factor in development of a humoral response is exposure to live P. haemolytica and prior exposure to the killed bacterium has no significant effect upon titre determined following aerosol challenge with live organisms.  (+info)

Activity in saline of phthalylated or succinylated derivatives of mycobacterial water-soluble adjuvant. (5/11816)

A water-soluble fraction (WSA) of the cell wall can substitute for mycobacterial cells in Freund complete adjuvant. However, when WSA is administered in saline instead of in a water-in-oil emulsion, its adjuvant activity is very weak, and under certain experimental conditions it can even inhibit the humoral immune response. The data reported in the present study show that after treatment by phthalic or succinic anhydride the adjuvant activity of WSA was markedly changed, since high levels of circulating antibodies were produced when these derivatives were administered with an antigen in an aqueous medium. Moreover, the antigenic determinants of WSA were modified and acylated WSA had no tuberculin-like activity.  (+info)

Immune response capacity after human splenic autotransplantation: restoration of response to individual pneumococcal vaccine subtypes. (6/11816)

OBJECTIVE: To evaluate features of general immune function, in particular the restoration of the humoral immune response to pneumococcal capsular polysaccharides, in humans undergoing a spleen autotransplantation after splenectomy because of trauma. SUMMARY BACKGROUND DATA: After splenectomy, patients have an increased risk of overwhelming infection or sepsis involving encapsulated bacteria such as pneumococci. The value of human spleen autotransplantation after splenectomy because of trauma has long been questioned. Mononuclear phagocyte system function appeared to be similar to that in splenectomized persons. The presence of specific antipneumococcal antibodies would allow other parts of the mononuclear phagocyte system, such as those in the liver, to phagocytose opsonized bacteria. METHODS: Ten consecutive patients undergoing splenectomy followed by autotransplantation were compared with the next 14 consecutive patients undergoing splenectomy alone. After a minimum of 6 months, the patients were vaccinated with 23-valent pneumococcal vaccine. Blood samples were taken at the time of vaccination and after 3 and 6 weeks for antipneumococcal capsular polysaccharides IgM and IgG enzyme-linked immunosorbent assay against types 3, 4, 6, 9, 14, and 23. Splenic regrowth was evaluated by scintigraphy. RESULTS: Surprisingly, several of the nonautotransplanted patients showed scintigraphic activity, indicating the presence of either accessory spleens or traumatic seeding (splenosis). Significant antibody titer increases (more than twofold) were found for both IgM and IgG in the autotransplanted patients. Splenectomized-only patients showed no significant increase in Ig levels in patients without splenic regrowth and partial improvement in patients with splenosis/accessory spleens. CONCLUSIONS: Considering this significant antipneumococcal antibody increase, spleen autotransplants can be expected to permit an adequate humoral response to pneumococcal infections and presumably also to other TI-2 antigens, and to protect against overwhelming postsplenectomy infection or sepsis.  (+info)

Helicobacter pylori infection, garlic intake and precancerous lesions in a Chinese population at low risk of gastric cancer. (7/11816)

BACKGROUND: Cangshan County of Shandong Province has one of the lowest rates of gastric cancer (GC) in China. While intestinal metaplasia (IM) and dysplasia (DYS) are less common in Cangshan than in areas of Shandong at high risk of GC, these precursor lesions nevertheless affect about 20% of adults age > or = 55. SUBJECTS AND SETTING: In order to evaluate determinants of IM and DYS in Cangshan County, a low risk area of GC a survey was conducted among 214 adults who participated in a gastroscopic screening survey in Cangshan County in 1994. METHOD: A dietary interview and measurement of serum Helicobacter pylori antibodies were performed. RESULTS: The prevalence of H. pylori was lowest (19%) among those with normal gastric mucosa, rising steadily to 35% for superficial gastritis (SG), 56% for chronic atrophic gastritis (CAG), 80% for IM, and 100% for DYS. The prevalence odds of precancerous lesions were compared with the odds of normal histology or SG. The odds ratio (OR) or CAG associated with H. pylori positivity was 4.2 (95% confidence interval [CI] : 1.7-10.0), while the OR of IM/DYS associated with H. pylori positivity was 31.5 (95% CI: 5.2-187). After adjusting for H. pylori infection, drinking alcohol was a risk factor for CAG (OR = 3.2, 95% CI: 1.1-9.2) and IM/DYS (OR = 7.8, 95% CI: 1.3-47.7). On the other hand, consumption of garlic showed non-significant protective effects and an inverse association with H. pylori infection. CONCLUSIONS: The findings of this study suggest that infection with H. pylori is a risk factor and garlic may be protective, in the development and progression of advanced precancerous gastric lesions in an area of China at relatively low risk of GC.  (+info)

Demographic, clinical and social factors associated with human immunodeficiency virus infection and other sexually transmitted diseases in a cohort of women from the United Kingdom and Ireland. MRC Collaborative Study of women with HIV. (8/11816)

BACKGROUND: Clinical experience suggests many women with HIV infection have experienced no other sexually transmitted diseases (STD). Our objective was to test the hypothesis that a substantial proportion of women with HIV infection in the United Kingdom and Ireland have experienced no other diagnosed STD and to describe the demographic, clinical and social factors associated with the occurrence of other STD in a cohort of HIV infected women. METHOD: Analysis of cross-sectional baseline data from a prospective study of 505 women with diagnosed HIV infection. The setting was 15 HIV treatment centres in the United Kingdom and Ireland. The main outcome measures were occurrence of other STD diagnosed for the first time before and after HIV diagnosis. Data were obtained from interview with women and clinic notes. We particularly focused on occurrence of gonorrhoea, chlamydia and trichomoniasis after HIV diagnosis, as these are the STD most likely to reflect recent unprotected sexual intercourse. RESULTS: The women were mainly infected via heterosexual sex (n = 304), and injection drug use (n = 174). 151 were black Africans. A total of 250 (49.5%) women reported never having been diagnosed with an STD apart from HIV, 255 (50.5%) women had ever experienced an STD besides HIV, including 109 (21.6%) who had their first other STD diagnosed after HIV. Twenty-five (5%) women reported having had chlamydia, gonorrhoea or trichomoniasis diagnosed for the first time after HIV diagnosis, possibly reflecting unprotected sexual intercourse since HIV diagnosis. In all 301 (60%) women reported having had sex with a man in the 6 months prior to entry to the study. Of these, 168 (58%) reported using condoms 'always', 66(23%) 'sometimes' and 56 (19%) 'never'. CONCLUSIONS: Half the women in this study reported having never experienced any other diagnosed STD besides HIV. However, after HIV diagnosis most women remain sexually active and at least 5% had an STD diagnosed which reflect unprotected sexual intercourse.  (+info)

*Autoimmune heart disease

Here, the anti-bacterial antibodies cross react with the heart antigens causing inflammation. Inflammatory damage leads to the ...

*Pharming (genetics)

Conducted field trials in US of feed peas for pigs that produced anti-bacterial antibodies. Former CSO is now with another ... a chimeric antibody produced in a hybridoma. Epicyte - spermicidal antibodies in corn Epicyte was purchased by Biolex in 2004 ... antibodies against Streptococcus mutans, antibodies against doxorubicin, and ICAM 1 receptor in tobacco PlantForm Corporation ... The product under development, ALT-836, formerly known as TNX-832 and Sunol-cH36, is the not the plant-produced antibody, but ...

*Bacterial display

Antibody epitope mapping is used to find the specificity of an antibody. The epitope (antibody binding site of antigens) is ... Bacterial surface display can be used for a variety of applications. These include affinity-based screening, antibody epitope ... Rockberg J, Lofblom J, Hjelm B, Uhlen M & Stahl S (2008). "Epitope Mapping of Antibodies Using Bacterial Surface Display". ... Bacterial display systems were first introduced by Freudl et al. and Charbit et al. in 1986, when they used bacterial surface ...

*Gerald Domingue

Domingue GJ, Acevedo HF, Powell JE, Stevens VC (1 July 1986). "Antibodies to bacterial vaccines demonstrating specificity for ... Domingue GJ, Schlegel JU (December 1978). "Novel bacterial structures in human blood. II. Bacterial variants as etiologic ... He first became interested in the role of atypical bacterial forms after noting that a large number of patients with urinary ... They noted that the forms, which they called electron dense bodies were so small that they could pass through bacterial filters ...

*Kary Mullis

"Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer". J. Mol. Med. 93 (6): ... Why not divert a fraction of those antibodies to the influenza strain you just picked up? A chemical linker synthesized with an ... The first proof-of-principle of this technology, re-targeting pre-existing antibodies to the surface of a pathogenic strep ... Buschman, Heather (May 6, 2015). "Molecular Homing Beacon Redirects Human Antibodies to Fight Pathogenic Bacteria" (Press ...

*George Siber bibliography

"Correlation between Serum IgG-2 Concentrations and the Antibody Response to Bacterial Polysaccharide Antigens". New England ... "Recurrent sinopulmonary infection and impaired antibody response to bacterial capsular polysaccharide antigen in children with ... "Antibody response to pretreatment immunization and post-treatment boosting with bacterial polysaccharide vaccines in patients ... Abstract P20). Siber GR, Santosham M, Priehs CM, Reid R, Letson W, Madore D and Eby R. Impaired antibody (Ab) response to H. ...

*List of MeSH codes (D12.776.124)

... antibodies, archaeal MeSH D12.776.124.486.485.114.107 -- antibodies, bacterial MeSH D12.776.124.486.485.114.107.288 -- ... antibodies, archaeal MeSH D12.776.124.790.651.114.125 -- antibodies, bacterial MeSH D12.776.124.790.651.114.125.288 -- ... antibodies, blocking MeSH D12.776.124.486.485.114.167 -- antibodies, catalytic MeSH D12.776.124.486.485.114.179 -- antibodies, ... antibodies, blocking MeSH D12.776.124.790.651.114.167 -- antibodies, catalytic MeSH D12.776.124.790.651.114.179 -- antibodies, ...

*Protein M

Hofer, Ursula (2014). "Bacterial physiology: Mycoplasmal protein binds antibodies". Nature Reviews Microbiology. 12 (4): 234- ... It is presumably a universal antibody-binding protein, as it is known to be reactive against all antibody types tested so far. ... It is capable of preventing the antigen-antibody interaction due to its high binding affinity to any antibody. The Scripps ... He found that M. genitalium was particularly responsive to all types of antibodies he tested from 20 patients. The antibody ...

*Cholera vaccine

The vaccine acts by inducing antibodies against both the bacterial components and CTB. The antibacterial intestinal antibodies ... Bacterial strains of both Inaba and Ogawa serotypes and of El Tor and Classical biotypes are included in the vaccine. Dukoral ... The anti-toxin intestinal antibodies prevent the cholera toxin from binding to the intestinal mucosal surface, thereby ...

*Passive immunity

... and is the most common antibody of the five types of antibodies found in the body. IgG antibodies protects against bacterial ... blood would be obtained for the antibodies. Patients who are immunized with the antibodies from animals may develop serum ... Antibody treatments can be time consuming and are given through an intravenous injection or IV, while a vaccine shot or jab is ... In humans, maternal antibodies (MatAb) are passed through the placenta to the fetus by an FcRn receptor on placental cells. ...

*Lyme disease microbiology

It is a two-strain, multiantigen vaccine which induces an antibody response to bacterial proteins OspA and OspC. Samuels DS, ... While each individual bacterial cell contains just one copy of the ospC gene, the gene sequence of ospC among different strains ... The bacterial membranes in at least the B31, NL303 and N40 strains of B. burgdorferi do not contain lipopolysaccharide, which ... However, rounded cell types triggered by an antibody binding to the OspB surface protein are damaged and dying forms of the ...

*Allotropes of oxygen

"Evidence for Antibody-Catalyzed Ozone Formation in Bacterial Killing and Inflammation". Science. 298 (5601): 2195-2199. Bibcode ...

*Regulatory B cells

However, peripheral blood Bregs were mostly CD24 and CD27 positive after cultivation with anti-CD40 antibody and CpG bacterial ... They were also positive for CD25, CD71 and PD-L1 after stimulation by CpG bacterial DNA and through TLR9. There are several ... In the 1970s it was noticed that Bregs could suppress immune reaction independently of antibody production. In 1996 Janeway´s ...

*Swine influenza

"Antibodies to selected viral and bacterial pathogens in European wild boars from southcentral Spain". Journal of Wildlife ... Antibodies to the virus are also common in pigs in other countries. The main route of transmission is through direct contact ... Of the 25 swine exhibitors aged 9 to 19 at the fair, 19 tested positive for antibodies to SIV, but no serious illnesses were ... Studies by the National Institutes of Health show a single dose creates enough antibodies to protect against the virus within ...

*Brucellosis

Identification of specific antibodies against bacterial lipopolysaccharide and other antigens can be detected by the standard ... Demonstration of antibodies against the agent either with the classic Huddleson, Wright, and/or Bengal Rose reactions, either ... B. ceti has been identified in four of the 14 cetacean families, but the antibodies have been detected in seven of the families ... False-negative SAT may be caused by the presence of blocking antibodies (the prozone phenomenon) in the α2-globulin (IgA) and ...

*Infectious bovine keratoconjunctivitis

This stimulate antibody production against the bacterial pilli to prevent their attachment and invasion of the conjuntiva. The ... and oversized or incorrectly placed ear tags may cause mechanical damage to the eye and facilitate bacterial colonization. The ...

*Mycoplasma hyorhinis

Bacterial culture, immunofluorescent antibody testing (IFAT), complement fixation test and haemagglutination can all be used to ... Type strain of Mycoplasma hyorhinis at BacDive - the Bacterial Diversity Metadatabase. ...

*Diagnostic microbiology

Antibodies are added to a bacterial solution, providing an antigen for the binding of fluorescent anti-antibody adherence. Mass ... Antibody detection works by attaching an indicator to an antibody with a known specificity and observing whether the antibody ... A benefit of antibody detection (ELISA) is that protein identification on a microorganism becomes faster than a western blot. ... By observing the ring formed around the disk formed due to the lack of bacterial growth, the zone of inhibition can be found, ...

*Single-domain antibody

The smaller size and single domain make these antibodies easier to transform into bacterial cells for bulk production, making ... is an antibody fragment consisting of a single monomeric variable antibody domain. Like a whole antibody, it is able to bind ... Single-domain camelids antibodies have been shown to be just as specific as a regular antibody and in some cases they are more ... In contrast to common antibodies, two out of six single-domain antibodies survived a temperature of 90 °C (194 °F) without ...

*Antigen

... named the hypothetical substances halfway between bacterial constituents and antibodies "substances immunogenes ou antigenes" ( ... In most cases, an adapted antibody can only react to and bind one specific antigen; in some instances, however, antibodies may ... Antigens are "targeted" by antibodies. Each antibody (immune response) is specifically produced by the immune system to match ... While antigens are the "target" of antibodies, immunoglobulin-binding proteins "attack" antibodies. . T-dependent antigen - ...

*HSP60

Bacterial HSP60 is causing the immune system to create anti-chaperonin antibodies, even though bacterial and human HSP60 have ... Antibodies against HSP60 targeted both the mitochondrial and cytoplasmic form. Nonetheless, antibodies against the signal ... These new antibodies are then recognizing and attacking human HSP60 which causes an autoimmune disease. This suggests that ... This suggests that the cell has different receptors and responses to human and bacterial HSP60. In addition, it has been shown ...

*Tuberculosis diagnosis

This skin patch contains antibodies recognizing the secreted bacterial protein MPB-64 passing through the blood capillaries of ... Antibodies from Lymphocyte Secretion or Antibody in Lymphocyte Supernatant or ALS Assay is an immunological assay to detect ... The principle is based on the secretion of antibody from in vivo activated plasma B cells found in blood circulation for a ... If the patch detects this secreted bacterial protein, the surrounding skin will redden. Two tests are available: the Mantoux ...

*Magnetotactic bacteria

Magnetotactic bacterial cells have been used to determine south magnetic poles in meteorites and rocks containing fine-grained ... the formation of magnetic antibodies, and the quantification of IgG; the detection and removal of Escherichia coli cells with a ... Magnetotactic bacterial magnetite crystals have been used in studies of magnetic domain analysis and in many commercial ... Bacterial magnetosome particles, unlike those produced chemically, have a consistent shape, a narrow size distribution within ...

*Salmonellosis

The Malawian researchers have identified an antibody that protects children against bacterial infections of the blood caused by ... A study at Queen Elizabeth Hospital in Blantyre found that children up to two years old develop antibodies that aid in killing ... Antibodies against nontyphoidal Salmonella were first found in Malawi children in research published in 2008. ... The high concentration on of antibodies of the yolk does not inhibit the Salmonella multiplication. Only seldom does ...

*Immunosurgery

... pathogen-free environment to increase the likelihood that the animal has not developed natural antibodies against the bacterial ... The trophoectoderm's cell junctions and tight epithelium "shield" the ICM from antibody binding by effectively making the cell ... rinsing it with hES derivation media to remove the antibodies, exposing it to complement, and then removing the lysed ...

*Phage display

Antibody phage display was later used by Carlos F. Barbas at The Scripps Research Institute to create synthetic human antibody ... Phage eluted in the final step can be used to infect a suitable bacterial host, from which the phagemids can be collected and ... HUMIRA, an antibody to TNF alpha, was the world's first fully human antibody, which achieved annual sales exceeding $1bn. Below ... The invention of antibody phage display revolutionised antibody drug discovery. Initial work was done by laboratories at the ...
E. coli antibody (FITC) for ELISA, FACS, ICC/IF. Anti-E. coli pAb (GTX40856) is tested in E. coli, Bacteria samples. 100% Ab-Assurance.
BioVendor - BioVendor Research and Diagnostic Products is a developer and manufacturer of immunoassays, recombinant proteins, antibodies and endotoxin-removal products.
BioVendor - BioVendor Research and Diagnostic Products is a developer and manufacturer of immunoassays, recombinant proteins, antibodies and endotoxin-removal products.
Blair, P B. and Lane, M, "Age- and tumor-correlated changes in functional antibody responses to mtv in neonatally infected mice." (1980). Subject Strain Bibliography 1980. 2387 ...
4. (Middle and Africa) Latin America Enzyme-Linked Immunosorbent Assay (ELISA) Market.. Report also includes Enzyme-Linked Immunosorbent Assay (ELISA) market growth rate XXXX % during forecast period. Worldwide Enzyme-Linked Immunosorbent Assay (ELISA) industry report covers competitors/Manufacturers Profiles in Enzyme-Linked Immunosorbent Assay (ELISA) market with their Business Overview. Enzyme-Linked Immunosorbent Assay (ELISA) Market report also includes Enzyme-Linked Immunosorbent Assay (ELISA) market by Type and Applications, Enzyme-Linked Immunosorbent Assay (ELISA) Sales, Revenue, Price and Enzyme-Linked Immunosorbent Assay (ELISA) Industry Share. This research (Enzyme-Linked Immunosorbent Assay (ELISA) Market Research) study also includes worldwide Enzyme-Linked Immunosorbent Assay (ELISA) Market Competition, by Manufacturer, by Manufacturer. Worldwide Enzyme-Linked Immunosorbent Assay (ELISA) Sales and Enzyme-Linked Immunosorbent Assay (ELISA) Revenue by Regions (2011-2016). Purchase ...
can be a pathogen with increasing severity for which host antibody responses provide protection from disease. changes Favipiravir in the rates and severity of disease generating renewed interest in novel approaches to disease treatment and prevention, including toxin-specific vaccines [4, 13C20]. It has been observed that toxin-specific, host antibodies influence the outcome of colonization and infection [21]. Patients with anti-toxin A antibodies at the time of colonization with spores are at lower risk of progression to active and severe disease [22]. Once infected, individuals who develop strong anti-toxin antibody responses clear their disease following antimicrobial treatment and remain disease free [23]. Such studies provide scientific rationale for development of a vaccine against toxins. While numerous studies have presented candidate vaccines [21, 24C28], to date, none has examined the DNA vaccine platform. DNA vaccination has a several advantages versus other modalities including ...
Booster responses to pertussis antigens [pertussis toxoid (PT), filamentous hemagglutinin (FHA), pertactin (PRN), and fimbriae types 2 and 3 (FIM)] were measured by enzyme-linked immunosorbent assay (ELISA). Booster responses were defined as participants with either a pre-vaccination antibody concentration less than lower limit of quantitation (,LLOQ), achieving a post-vaccination level ≥4X LLOQ, or pre-vaccination antibody concentrations ≥LLOQ but ,4X LLOQ, achieving a 4-fold rise rate of post-vaccination, or a pre-vaccination antibody concentration ≥4X LLOQ, achieving a 2-fold response ...
The anti-Pertussis concentration were determined by ELISA. The criteria for demonstrating booster response are: (i) Pre-vaccination antibody concentrations less than the lower limit of quantitation (LLOQ) for each anti-pertussis antibody (PT, FHA, FIM, and PRN) but a post-vaccination levels ≥ 4 x LLOQ; or (ii) Pre-vaccination antibody concentrations ≥ LLOQ but , 4 x LLOQ with a 4-fold rise rate; or (iii) Pre-vaccination antibody concentrations ≥ 4 x LLOQ but with a 2-fold rise rate ...
With the Antibody Dynamics platform, investigators have access to technologies that allow the interrogation of the biophysical and functional properties of antibody isotypes and subclasses to a variety of global health diseases. These properties can lead to the identification of correlates of protection, which is essential for enabling product development and licensure of highly efficacious vaccines. Moreover, characterization of antibody responses can enable a greater understanding of the mechanistic underpinnings of immune protection, leading to further innovations in therapeutic and prevention strategies to improve human health.. The Antibody Dynamics platform uses customizable, antigen-specific binding, avidity, and functional assays in a GCLP-compliant environment to profile biophysical and functional antibody responses to infection and vaccination. Profiling is available for all antibody isotypes and subclasses and for several global health diseases, including malaria, TB, hepatitis B, ...
The kits are optimized for labeling antibodies with a concentration between 0.5-1.0 mg/mL. If your antibody solution is too dilute, you can concentrate it by centrifugation using the ultra-filtration vial provided in the kit. If your antibody solution is too concentrated, you can dilute it with 1x PBS. Antibody concentrations outside the recommended range may result in either under or over labeling ...
Used Mercedes G500 For Sale - 28 images - Used 2013 Mercedes G Class For Sale, Used G500 Mercedes Sale, Used 2015 Mercedes G Class For Sale In Essex, 2005 Mercedes G500 For Sale White On Black Beverly, Used Mercedes G Class For Sale Buy Cheap Pre Owned
RESULTS:. In relation to HBV, the study showed 0.5% of carriers, 8.3% with a previous infection profile, 1.1% with a previous or current infection profile, and 33.6% with a vaccine response, characterizing that municipality as low endemicity for this virus. HBV DNA was detected in 66.7% of the HBsAg+ samples, with viral load between , 55 IU/mL and , 38,000 IU/mL; 69.2% of these samples were A1 subgenotype, 15.4% F2, and 15.4% F4. Of the total, 1.1% were samples from individuals anti-HBc total+ isolated whose molecular biology tests showed that 9.3% had HBV DNA, with viral load between , 15 IU/mL and 48 IU/mL. ...
Shop Weak toxin ELISA Kit, Recombinant Protein and Weak toxin Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
There is no contraindication for receiving steroid injections when someone is HIV positive. Current infection would mean having an additional active infection, particularly in the area where...
Optimizing the concentration of primary and secondary antibodies prior to Western blot leads to improved data, a reduction in time and savings in valuable resources.
MSP1-19 particular rabbit antisera plus the AMA1 MAb were when compared in one cycle and two cycle development inhibition assays to ascertain whether or not a next cycle of parasite advancement would boost the sensitivity of your assay. Growth from the PcMSP1-19 parasite line in the presence of inhibitory antibodies confirmed that a next cycle elevated antibody mediated development inhibition for both equally the PcMSP1-19 and AMA1 antibodies but not the PfMSP1-19 antibodies (Figure 3A ...
Patients will not be able to join the trial if:. · Infection not caused by S. aureus alone in the opinion of the infection specialist (e.g. S. aureus is considered a blood culture contaminant, or polymicrobial culture with another organism likely to be contributing clinically to the current infection ...
I have A- blood and my Dr. sent me to get an antibody screening since we dont know the fathers blood type.he told me to go 2 days before my appt. So I went Monday.when I went to the Dr.my results hadn...
This website contains information on products which is targeted to a wide range of audiences and could contain product details or information otherwise not accessible or valid in your country. Please be aware that we do not take any responsibility for accessing such information which may not comply with any legal process, regulation, registration or usage in the country of your origin ...
The introduction of vaccines containing the capsular polysaccharides of N. meningitidis, S. pneumonia, and H. influenzae type b has driven a significant reduction in cases of disease caused by these bacteria. The polysaccharide-specific antibody responses following vaccination are well characterized, however less is known about the B cells underlying this response. Here, we summarize the plasma cell (PC) and memory B cell (BMEM) responses following plain polysaccharide and protein-polysaccharide conjugate vaccination, drawing together studies covering a range of vaccines and age groups. These studies show that infant primary PC and BMEM responses to polysaccharide-conjugate vaccines are low in relation to older age groups but are significantly higher following booster doses. PC kinetics have generally been found to follow a similar pattern irrespective of vaccine type or age group, whereas divergent BMEM responses have been reported following plain polysaccharide and conjugate vaccination. A degree of
Shop Anti-lipopolysaccharide factor ELISA Kit, Recombinant Protein and Anti-lipopolysaccharide factor Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
An abnormal result means you have a current infection with C burnetii, or that you have been exposed to the bacteria in the past. People with past exposure may have antibodies, even if they are not aware that they were exposed. Further testing may be needed to distinguish between current, previous, and chronic infection. During the early stage of an illness, few antibodies may be detected. Antibody production increases during the course of an infection. For this reason, this test may be repeated several weeks after the first test. ...
A free platform for explaining your research in plain language, and managing how you communicate around it - so you can understand how best to increase its impact.
There have been many attempts to identify functional activity of serum or salivary antibodies in vitro and in addition some conclusions can be drawn from in
Looking for medication to treat infection+of+the+blood+caused+by+klebsiella+bacteria? Find a list of current medications, their possible side effects, dosage, and efficacy when used to treat or reduce the symptoms of infection+of+the+blood+caused+by+klebsiella+bacteria
Enlightened veterinarians and pet parents have become increasingly wary of the health risks, and lack of benefits, associated with repeatedly vaccinating dogs after their initial "puppy shots." Is titer testing the solution to the over-vaccination problem? Heres a crash course to help you muddle through the mire of misinformation surrounding this simple blood test, and to help you decide whether or not to test your dogs antibody titers.. What is titer testing?A titer test (pronounced TIGHT er) is a laboratory test measuring the existence and level of antibodies to disease in blood. Antibodies are produced when an antigen (like a virus or bacteria) provokes a response from the immune system. This response can come from natural exposure or from vaccination. (Note: titering is also called serum vaccine antibody titering and serologic vaccine titering.). How is the test performed? Your test result will have an explanation of what your pets test result means. But if you want to know more, heres ...
View Notes - micro report 4 from MICRO 400 at Kansas State University. Enzyme-Linked Immunosorbent Assay Matthew Ricke TA: Meg and Smita I ntroduction: The ELISA test is an Antibody based test. The
Specific antibodies against p185. (A) Anti p185-antibody titre in vaccinated and control animals. Mean antibody titres are indicated by full line (**P=0.0024).
Comparative antibody response to vaccine candidates.(A) Individual serum antibody titers towards wild-type Hla determined after three immunizations (B) Neutrali
Complete Report Details @ https://marketreportscenter.com/reports/159995/global-enzyme-linked-immunosorbent-assay-elisa-industry-2016-market-research-report. Regional Insights:. The report lets you have an edge across the targeted regions with the comprehensive competitive framework. It analyzes the market on the basis of segmentation at a regional level coupled with price rate, profit, forecast, and estimates. The report studies the use of Enzyme-Linked Immunosorbent Assay (ELISA) across several sectors to study and projects the future growth prospects. The report covers regional analysis of the market with respect to the existing market size and future prospects. It features historical stats, data and revenue estimation of the market segments and sub-segments in accordance with the top geographic regions and their countries. It discusses the current scenario of the Enzyme-Linked Immunosorbent Assay (ELISA) market across major geographic segments North America, China, Europe, Asia (Ex. China) ...
The absence of PspA or presence of antibody to PspA facilitates the complement-dependent phagocytosis of pneumococci in vitro.: Pneumococcal surface protein A (
Recently I run on a FACS analysis using simply the FITC conjugated antibody directly stained against one of the membrane proteins of my cells. I did twice. The first time I used almost 1:150 FITC conjugated antibody and the results showed no signals. So I stained my cells with diluted antibody at the concentration of 1:5, 1: 10 and 1:20 at the second time. Although the results showed very high positive signals, I doubted about it. It looked like the percentage of the positive cells decreased with the antibody concentration. The only explanation is that the antibody concentration is too high and most of the signals come from the background. Am I right ...
Research Report on Global Enzyme-Linked Immunosorbent Assay (Elisa) Testing Market 2016 Industry, Analysis, Research, Share, Growth, Sales, Trends, Supply, Forecast to 2021. The Report includes market price, demand, trends, size, Share, Growth, Forecast, Analysis & Overview.. Send Your Enquiry @ http://www.qyresearchreports.com/sample/sample.php?rep_id=743302&type=E ...
You will not be accepted as a blood donor if you are taking drugs for any current infections or heart diseases, or are undergoing cancer treatment.. Please postpone your donation:. ...
compare the 2013 Toyota Land Cruiser with 2013 Mercedes-Benz G Class, side by side. See rating, reviews, features, prices, specifications and pictures
Rabbit polyclonal Endo G antibody validated for WB and tested in Human and Mouse. Immunogen corresponding to recombinant fragment
Rabbit polyclonal Endo G antibody validated for WB, ELISA, IHC, ICC/IF and tested in Human, Mouse and Rat. Referenced in 20 publications and 3 independent…
Gentaur molecular products has all kinds of products like :search , Kamiya \ Mouse Reference Serum 3 \ KT-527 for more molecular products just contact us
anti-PRP39a | premRNA-processing factor 39 antibodies, At1g04080, AS14 2784, Q93ZR3, F4I448, RNA processing, mRNA 5-splice site recognition, regulation of timing of transition from vegetative to reproductive phase
Does anyone know of a supplier for an antibody (polyclone or monoclone) to E. coli (either the whole bacteria or bacteria fragment/ epitope)? Thanks in advance. /==========*===========*===========*===========*===========*=========*========, , ___ , , /, /, , , / , mlwillia at apgea.army.mil , , / , / , , ,/ ,__ Chemical Research Development and , , / , / , , , \ , Engineering Center , , / , / , , , \ ,___ Aberdeen Proving Ground, MD USA , , , ,==========*===========*===========*===========*===========*=========*========, ...
This case involves transfusing a patient who has a positive antibody screen with crossmatch-compatible RBC before the antibody has been identified. A...
マウス・モノクローナル抗体 ab4066 交差種: Ms,Rat,Cow,Hu 適用: WB,IHC-P,IHC-Fr,ICC/IF…S100抗体一覧…画像、プロトコール、文献などWeb上の情報が満載のアブカムの Antibody…
ヤギ・ポリクローナル抗体 ab14708 交差種: Hu 適用: WB,IHC-P…S6K1抗体一覧…画像、プロトコール、文献などWeb上の情報が満載のアブカムの Antibody 製品。国内在庫と品質保証制度も充実。
titers - MedHelps titers Center for Information, Symptoms, Resources, Treatments and Tools for titers. Find titers information, treatments for titers and titers symptoms.
This page highlights the benefits of the recently developed strong emitting dioxetanes (AquaSpark(TM)), which are ideal for applications in enzyme linked immunosorbent assays (ELISA).
TY - JOUR. T1 - Chlamydia pneumoniae serology. T2 - Interlaboratory variation in microimmunofluorescence assay results. AU - Peeling, Rosanna W.. AU - Wang, San Pin. AU - Grayston, J. Thomas. AU - Blasi, Francesco. AU - Boman, Jens. AU - Clad, Andreas. AU - Freidank, Heike. AU - Gaydos, Charlotte A.. AU - Gnarpe, Judy. AU - Hagiwara, Toshikatsu. AU - Jones, Robert B.. AU - Orfila, Jeanne. AU - Persson, Kenneth. AU - Puolakkainen, Mirja. AU - Saikku, Pekka. AU - Schachter, Julius. PY - 2000. Y1 - 2000. N2 - The lack of standardization in chlamydia serology has made interpretation of published data difficult. This study was initiated to determine the extent of interlaboratory variation of microimmunofluorescence (MIF) test results for the serodiagnosis of Chlamydia pneumoniae infections. Identical panels of 22 sera were sent to 14 laboratories in eight countries for the determination of IgG and IgM antibodies by MIF. Although there was extensive variation in the numeric titer values, the overall ...
Streptococcus pneumoniae is a serious worldwide pathogen and the focus of numerous vaccine development projects. Currently the most widely accepted surrogate marker for evaluating the efficacy of a given vaccine is to utilize ELISA. Measurement of antibody concentration by ELISA without reduction in cross-reactive antibodies causes an overestimation of antibody concentration and therefore protection, this is most notable in the aged, an at risk group for this infection. We compared the immune response to the pneumococcal polysaccharides (PPS) 4 and 14 of 20 young to 20 elderly adults. Pre-and post-vaccination IgG antibody concentrations and antibody avidity against PPS4 and PPS14 were measured using two different enzyme-linked immunosorbant assay (ELISA) absorption protocols. All sera were pre-absorbed with either cell-wall polysaccharide (CPS), or CPS and serotype 22F polysaccharide. Pre- and post-vaccination IgG antibody concentrations for serotype 4, but not 14, were significantly lowered with the
Where to buy Human group A streptococcal polysaccharide antibody ELISA Kit? Human group A streptococcal polysaccharide antibody ELISA Kit Suppliers,Human group A streptococcal polysaccharide antibody ELISA Kit Prices on Green Stone - medicinerawmaterials.com.
Repeated immunisation with the experimental vaccine lead to increasing levels of specific serum antibodies in the vaccinated rainbow trout. The farmed rainbow trout responded with high antibody levels to a single injection with the commercial vaccine. However, the diversity in responses was more pronounced in the farmed fish. Primary visual examinations for vaccine status in rainbow trout from the commercial farm revealed a large pool of unvaccinated specimens (vaccination failure rate = 20%) among the otherwise vaccinated fish. Through serum analyses using the ELISA in a blinded set-up it was possible to separate samples collected from the farmed rainbow trout into vaccinated and unvaccinated fish ...
The invention described herein provides human antibodies produced in non-human animals that specifically bind to Streptococcus pneumoniae capsular polysaccharide (PPS-3). The invention further provides methods for making the antibodies in a non-human animal and for expressing the antibodies in cells including hybridomas and recombinant host cell systems. Kits and pharmaceutical compositions comprising the antibodies are also provided in addition to methods of treating, inhibitng or preventing S. pneumoniae infection or conditions or disorders caused by such infection by administering to a patient the pharmaceutical compositions described herein.
Anti-Borrelia burgdorferi antibody conjugated to FITC validated for IHC, ICC/IF. Immunogen corresponding to tissue, cells or virus
This is the first comprehensive report of serologic responses to the oral ETEC-rCTB vaccine in subjects living in an area where ETEC is endemic. We consider the pediatric data presented herein especially relevant, since children and infants in Egypt and other developing countries represent important targets for ETEC immunization. Several of our findings bear importance for future evaluations of the ETEC-rCTB vaccine. First, the vaccine elicited pronounced antitoxin antibody responses of both IgA and IgG isotypes in all three age groups as reported here and elsewhere (15). Second, a high proportion of vaccinated children achieved seroconversion to each of the four vaccine-shared CF antigens studied, and the magnitude of antibody elevations, particularly of IgA isotype, was substantial. Third, both age and preimmunization titer were independently and inversely associated with the magnitude of IgA antibody responses to the vaccine. Last, IgG antibodies to CF antigens appeared to reflect a ...
Chlamydia trachomatis antibody [103] for ELISA. Anti-Chlamydia trachomatis mAb (GTX40767) is tested in Chlamydia samples. 100% Ab-Assurance.
Effect of anti-γ chain and anti-µ chain on secondary antibody response of rabbit spleen cells was studied in vitro. Spleen cells from rabbits primed with dinitrophenylbovine γ globulin (DNP-BGG) were stimulated in vitro with the antigen either in the presence or absence of anti-heavy chain antibody. After 24 hr of incubation, the cells were washed and cultured in the absence of the antigen. Antibody response was evaluated by enumerating IgG and IgM antibody producing cells and by measuring anti-DNP antibodies in culture media. It was found that anti-γ chain suppressed both IgG and IgM antibody response, while anti-µ chain suppressed only IgM antibody response. When the primed cells were cultured without antigen stimulation, IgM antibody producing cells increased after 3 days culture. The response was also suppressed by treatment of the cells by either anti-γ or anti-µ chain. The treatment with anti-heavy chains did not inhibit secretion of antibodies from antibody producing cells. It ...
Salivary and serum antibody response against Neisseria meningitidis after vaccination with conjugate polysaccharide vaccines in Ethiopian ...
Efficiency of a Pneumococcal Opsonophagocytic Killing Assay Improved by Multiplexing and by Coloring Colonies: For evaluating pneumococcal vaccines, the opsonop
The Antibody Resource Page (http://www.antibodyresource.com/) is an invaluable website to researchers and educators. Here is just some of what can be found on the page: 1. How to Find an Antibody - a variety of ways on and off the web to find the antibody you are looking for. There are links to free search engines that allow you to search a multitude of companies for the specific antibody you need. 2. Online Companies - links to over 110 companies that sell antibodies or antibody related products. Is your company listed on this page? 3. Antibody Image Gallery - animated antibody pictures are available 4. Bulletin Board - Have a question? Then stop by and post a message. 5. Educational Resources - a variety of new links have been added.There are links to pages on immunochemistry, antibody production, autoimmunity, vaccines, immunology and much more. This page is divided up into sections on research, educational, and health resources. 6. The latest in antibody news - Get up-to-date, ...
IgA subclass antibodies have been reported in a few studies in patients with CAC. IgA antibodies and IgA1 antibodies were increased in comparison with the
Mouse Monoclonal Anti-Francisella Tularensis LPS Antibody (T14) [DyLight 405]. Validated: ELISA, ICC/IF. Tested Reactivity: Bacteria. 100% Guaranteed.
Vol 6: Engineering Venoms Toxin-Neutralizing Antibody Fragments and Its Therapeutic Potential.. This article is from Toxins, volume 6.AbstractSerum therapy remains the only specific treatment against envenoming, but . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Baker, P J.; Prescott, B; Stashak, P W.; and Amsbaugh, D F., "Characterization of the antibody response to type iii pneumococcal polysaccharide at the cellular level. Iii. Studies on the average avidity of the antibody produced by specific plaque-forming cells." (1971). Subject Strain Bibliography 1971. 1026 ...
Looking for protective antibody? Find out information about protective antibody. protein produced by the immune system in response to the presence in the body of antigens: foreign proteins or polysaccharides such as bacteria, bacterial... Explanation of protective antibody
Since antibodies are only produced after exposure to foreign material including organisms such as bacteria and viruses, they indicate exposure to that organism.. Because antibodies remain in circulation for quite some time, the presence of an antibody titre to a particular organism does not necessarily mean that that organism is the cause of the illness that your pet is experiencing.. The lack of an antibody titre to an organism can indicate either of two things. The first is that no exposure to that organism has occurred and therefore your pets current clinical condition is due to another cause. The other is that your pet has been so recently exposed to the organism that there has been insufficient time for antibodies to be produced in sufficient quantities to be detectable in the serum.. Therefore if we suspect that a particular disease (for example leptospirosis) is causing illness in your pet, it is important to obtain both acute and convalescent serum titres. This simply means that blood ...
Synonyms for antibodies in Free Thesaurus. Antonyms for antibodies. 21 words related to antibody: active site, protein, autoantibody, precipitin, ABO antibodies, Rh antibody, antitoxin, agglutinin, Forssman antibody.... What are synonyms for antibodies?
Synonyms for antititin antibody in Free Thesaurus. Antonyms for antititin antibody. 21 words related to antibody: active site, protein, autoantibody, precipitin, ABO antibodies, Rh antibody, antitoxin, agglutinin, Forssman antibody.... What are synonyms for antititin antibody?
We will use appropriate corrections to reduce the probability required to accept statistical significance. Geometric means of the antibody concentrations (GMC) of ELISA (micro-gram/millilitre) and geometric mean titers (GMT) of OPA (titer-1) at each of the five visits and geometric mean fold rise (GMR) between visits will be calculated within each treatment group for each of the 7 pneumococcal serotypes (4,6B, 9V, 14, 18C, 19F and 23F), and 95% confidence intervals will be constructed by back transformation of the confidence intervals for the mean of the log transformed assay results computed using the Student t distribution. Comparisons of geometric mean antibody concentrations and titers measured by ELISA and OPA, respectively, between the immunization groups were performed by unpaired 2-tailed t-test for parametric analyses and Wilcoxon rank sum test for non-parametric analyses. Based on changes of GMC of ELISA and GMT of OPA at five time points (visits), the rate of wanning immunity can be ...
An antigen is a substance which is used to generate the production of antibodies. In the case of this PolyExpressTM package, this is either a native protein or peptide sequence. The origin of your target antigen is the type of organism that the protein or peptide sequence to be used as an antigen originally came from. An accession number is a unique identification given to a biological polymer sequence and may be found in protein databases such as UniProt. Peptides created through GenScripts OptimumAntigen™ design program have many advantages over full proteins when it comes to antibody production. Our OptimumAntigen™ Design Tool combines the industrys most advanced algorithms with GenScripts time-tested expertise. Each peptide antigen is measured against several protein databases to confirm the desired antibody and epitope specificity. We guarantee a polyclonal antibody with ELISA titer of 1:32,000 or better for any host with the aid of our antigen design tool and unique antibody ...
Validated Anti-Phospho-ERBB4-Y1284 antibody from Abcams OEM supplier ABclonal, validated in WB. ABclonal is epicypher validated ChIP grade Histone antibody provider. AntibodyPlus provides trial size antibody samples for antibody validation. Replacement to Abcam, Santa Cruz, Sigma and CST antibody.
Validated Anti-CALHM1 antibody from Abcams OEM supplier ABclonal, validated in WB,IHC. ABclonal is epicypher validated ChIP grade Histone antibody provider. AntibodyPlus provides trial size antibody samples for antibody validation. Replacement to Abcam, Santa Cruz, Sigma and CST antibody.
Enzyme immunoassay for the determination of IgG antibodies to Pneumococcal Capsular Polysaccharide (PCP) in human serum or plasma.
Enzyme immunoassay for the determination of IgG antibodies to Pneumococcal Capsular Polysaccharide (PCP) in human serum or plasma. SmartEIA kit is specifically designed for automated analysis using the Agility instrument.
Laying hens (ISA Brown) were divided into 5 groups with 7 birds each. Group 1 was a nonimmunized control, whereas birds in group 2 were immunized intravenously with SRBC, and those in groups 3, 4, and 5 were treated intraperitoneally with propolis (2, 10, and 50 mg/kg, respectively) on 3 consecutive days and then inoculated intravenously with SRBC. Hematological and serological analyses were carried out on d 0, 3, and 38. Natural and specific antibody levels were determined by hemagglutination with rabbit red blood cells and SRBC, respectively ...
Gentaur molecular products has all kinds of products like :search , BBridge \ anti_ PCNA antibody, rabbit serum anti_ PCNA antibody, rabbit serum \ 70-080 for more molecular products just contact us
You are hot in pursuit of an interesting protein but there are no commercial antibodies or they are bad. Its time to think about raising your own antibodies against the protein. It will be a time and money consuming undertaking but once you developed a specific antibody or set of antibodies, you will be in a unique position to generate interesting data. ...
Anti-PrP, clone DRM2-118 Antibody Anti-PrP, clone DRM2-118 | MABN768 is an antibody against PrP for use in Western Blotting, Immunohistochemistry, ELISA. - Find MSDS or SDS, a COA, data sheets and more information.
ELISA, Enzyme Linked Immunosorbent Assay is an immunochemical measurement method where antibodies or antigens that are measured have been labeled with enzymes. The presence of antigen or antibody is detected by the ability of the enzymes to e.g. induce a color change in the test.. The assay format combine the specificity of antibodies with the sensitivity of simple enzyme assays, by using antibodies or antigens coupled to an easily-assayed enzyme. ELISAs can provide a useful measurement of antigen or antibody concentration. ...
This program of research employs a Natural Exposure Advertising Research method to assess smokers responses to a series of televised anti-smoking ads in Victoria.
monoclonl antibody application specifity - posted in ELISA and Immunoassay: Hi how can antibodies become specific for an application for example western blotting or immunopercipitation? what if I use an antibody that specified for WB by supplier in other application like elisa? regards
Mouse Monoclonal S100A9 antibody for EIA, FACS, IHC (fro), IHC (p). Published in 8 Pubmed References. Order this anti-S100A9 antibody. | Product number ABIN111886
Pathogenic bacteria are usually destroyed by antibodies, but a lung-damaging bacterium turns the bodys antibody response in its favour, suggests a study published.
A normal level of IgG antibodies in an adult is between 700 and 1500 milligrams per deciliter, according to WebMD. A high level may indicate a chronic infection or other medical condition, while a...
This antibody pair set comes with matched antibody pair to detect and quantify protein level of human GTF2A1L. (H00011036-AP11) - Products - Abnova
G090 Anti-EMR1 Antibody detects endogenous levels of total EMR1 protein. Validated for WB and ELISA. Reactive for Human. AssayBiotechnology an original antibody manufacturer.
C30132 Anti-STRA8 Antibody detects endogenous levels of STRA8 protein. Validated for WB and ELISA. Reactive for Human. AssayBiotechnology an original antibody manufacturer.
This antibody pair set comes with matched antibody pair to detect and quantify protein level of human DCTN2. (H00010540-AP21) - Products - Abnova
Anyone in research antibody business will know that discussions relating to antibody quality and validation has been increasing over the last couple of years.
Find and order matched antibody pairs and products like this EWSR1 Antibodies Pair on www.antibodies-online.cn. 产品编号 ABIN2441366.
Find and order matched antibody pairs and products like this AIPL1 (Human) IP-WB Antibody Pair on www.antikoerper-onli... | Jetzt Produkt ABIN1343163 bestellen.
Find and order matched antibody pairs and products like this USP33 (Human) IP-WB Antibody Pair on www.antikoerper-onli... | Jetzt Produkt ABIN1343095 bestellen.
Cancer, aqueous and serum antibody levels may also be compared oline generate Desmontes numbers (the ratio рnline aqueous to serum buy real Snovitra online levels).Nakajima, H. 4 Linear Accelerators Another interesting concept merging the continuous beam production of a cyclotron and the flexibility in energy selection offered by RF acceleration systems has been proposed and developed by U.
FGF1-Specific Antibody 16873-1-AP has been identified with IF, ELISA. 16873-1-AP detected band in {{ptg:PositiveWB}} with {{ptg:WesternTiter}} dilution...
Creative Biolabs now provides all-round service of anti-GPCR membrane protein antibody discovery service against a variety of GPCR targets.
GTF2F2 Antibody 15139-1-AP has been identified with IF, WB, ELISA. 15139-1-AP detected 30 kDa band in K-562 cells with 1:500-1:3000 dilution...
抗體(英語4antibody(3又稱免疫球蛋白(immunoglobulin3簡稱Ig(3是一種由B淋巴細胞分泌3被免疫系統用來鑒別與中和外來物質如細菌 病毒等的大型Y形蛋白質3僅被發現存在於脊椎動物的血液等體液中3及其B細胞的細胞膜表面》抗體能識別特定外來物的一個獨特特徵3該外來目標被稱為抗原》蛋白上Y形的其中兩個分叉頂端都有一被稱為互補位(抗原結合位(的鎖狀結構3該結構僅針對一種特定的抗原表位》這就像一把鑰匙只能開一把鎖一般3使得一種抗體僅能和其中一種抗原相結合》 抗體和抗原的結合完全依靠非共價鍵的相互作用3這些非共價鍵的相互作用包括氫鍵 范德華力 ...
ASTL Antibody 21418-1-AP has been identified with ELISA, WB. 21418-1-AP detected 43 kDa band in HEK-293 cells with 1:200-1:1000 dilution...
Anti-polysaccharide immunity is a key facet of protection against several bacterial pathogens. Problems exist with current polysaccharide vaccines and alternative strategies that deliver a protective response are needed. We have identified immunological peptide mimics of type 6B and 9V pneumococcal capsular polysaccharides that could be used as vaccine antigens. Peptides mimicking antigenic properties of serotype 6B capsular polysaccharide were obtained from a phage-displayed peptide library expressing dodecameric peptides, using a human monoclonal antibody (Db3G9). A murine monoclonal antibody (206, F-5) against the serotype 9V capsular polysaccharide identified three peptide mimotopes from the dodecameric peptide library and one from a random pentadecameric peptide library. In ELISA, binding of 206, F-5 and Db3G9 to phage displaying the selected mimotopes was significantly inhibited by type-specific pneumococcal polysaccharide. Peptides were conjugated to keyhole limpet haemocyanin and were ...
BioAssay record AID 289164 submitted by ChEMBL: Inhibition of Bacillus anthracis protective antigen 63 reconstituted in diphytanoylphosphatidylcholine lipid membranes assessed as inhibition of channel conductance at 5 uM.
Pneumovax® 23 (23-valent pneumococcal polysaccharide vaccine, 23vPPBV) (1 dose), Pneumovax 23, Swindon Medical Company Limited. Administered a single dose to 2 years of age and older.Pneumonia is the 2nd leading cause of death in HK. Streptococcus pneumoniae is one of the common bacteria causing pneumonia (lung infection) and serious diseases; for example, meningitis, otitis media, sepsis and even death.According to the recommendations of the Hong Kong Centre for Health Protection: All aged 2 or above or high-risk elderly persons aged 65 or above should have pneumococcal vaccination, and high-risk individuals should be vaccinated with a 13-valent conjugate -The vaccination injection process is handled by medical professional.
TURNBULL, P.C.B. et al. Naturally acquired antibodies to Bacillus anthracis protective antigen in vultures of southern Africa. Onderstepoort j. vet. res. [online]. 2008, vol.75, n.2, pp.95-102. ISSN 2219-0635.. Sera from 19 wild caught vultures in northern Namibia and 15 (12 wild caught and three captive bred but with minimal histories) in North West Province, South Africa, were examined by an enzyme-linked immunosorbent assay (ELISA) for antibodies to the Bacillus anthracis toxin protective antigen (PA). As assessed from the baseline established with a control group of ten captive reared vultures with well-documented histories, elevated titres were found in 12 of the 19 (63 %) wild caught Namibian birds as compared with none of the 15 South African ones. There was a highly significant difference between the Namibian group as a whole and the other groups (P , 0.001) and no significant difference between the South African and control groups (P , 0.05). Numbers in the Namibian group were too small ...
Question: Some of the physicians I work with are reluctant to administer 23-valent pneumococcal polysaccharide vaccine (PPSV23) to adults, stating that it is not very effective.
TY - JOUR. T1 - Immunogenicity and safety of concomitant MF59-adjuvanted influenza vaccine and 23-valent pneumococcal polysaccharide vaccine administration in older adults. AU - Song, Joon-Young. AU - Cheong, Hee-Jin. AU - Tsai, T. F.. AU - Chang, Hyun ah. AU - Choi, Min Joo. AU - Jeon, Ji Ho. AU - Kang, Seong Hee. AU - Jeong, Eun Ju. AU - Noh, Ji Yun. AU - Kim, Woo Joo. PY - 2015/1/1. Y1 - 2015/1/1. N2 - Background: Concomitant administration of influenza and pneumococcal vaccines facilitates their uptake by older adults; however, data on immunogenicity and safety of concomitant administration of adjuvanted trivalent inactivated influenza vaccine (aIIV3) and 23-valent pneumococcal polysaccharide vaccine (PPSV23) have not been reported. Methods: Subjects aged ≥65 years (. N=. 224) were randomized 1:1:1:1 to receive MF59-aIIV3 alone, MF59-aIIV3. +. PPSV23 in contralateral arms, MF59-aIIV3. +. PPSV23 in the same arm or PPSV23 alone (Clinical Trial Number - NCT02225327). Hemagglutination ...

Occurrence of antibodies to Borrelia burgdorferi in patients with nonspirochetal subacute bacterial endocarditis.Occurrence of antibodies to Borrelia burgdorferi in patients with nonspirochetal subacute bacterial endocarditis.

To determine the prevalence and specificity of antibodies to Borrelia burgdorferi in patients with nonspirochetal subacute ... Occurrence of antibodies to Borrelia burgdorferi in patients with nonspirochetal subacute bacterial endocarditis.. * ... Only 1 of 20 patients with rheumatoid arthritis without known bacterial infections had antibodies to B. burgdorferi. ... Cross-reactive antibodies to shared epitopes between B. burgdorferi and the endocarditis organism may account for the high ...
more infohttp://www.prohealth.com/library/showarticle.cfm?libid=26987

Controlling The Lyme Disease Epidemic - Science FridayControlling The Lyme Disease Epidemic - Science Friday

JOHN AUCOTT: So the current diagnostic test is based on antibody production. Those antibodies take about two or three weeks to ... This is a bacterial infection. And the standard treatment is with antibiotics. There are a subset of people that- actually, ... After two or three weeks of infection, then the antibodies form, and then the test starts to work better. But there is this gap ... Were relying on these antibodies, which are made by your immune system. And they persist for long periods of time after the ...
more infohttps://www.sciencefriday.com/segments/controlling-the-lyme-disease-epidemic/

Bacterial EndocarditisBacterial Endocarditis

... , Infective Endocarditis, Infectious Endocarditis, Subacute Bacterial Endocarditis, Acute Endocarditis, ... Bartonella serology (Antibody titers ,1:800) or tissue biopsy PCR. *Consider in culture negative cases, esp Alcoholism or ... Endocarditis bacterial, Bacterial endocarditis, BE - Bacterial endocarditis, Bacterial endocarditis (disorder), bacterial; ... Bacterial Endocarditides, Bacterial Endocarditis, Endocarditides, Bacterial, Endocarditis, Bacterial, ENDOCARDITIS BACT, BACT ...
more infohttp://www.fpnotebook.com/CV/ID/BctrlEndcrdts.htm

Human monoclonal antibodies against bacterial toxins - University of RochesterHuman monoclonal antibodies against bacterial toxins - University of Rochester

The production of stable hybrid cell lines that secrete human monoclonal antibodies against bacterial toxins by fusing post- ... SUMMARY OF CLONED MOUSE-HUMAN HYBRID CELL LINES Tissue Culture Duration of Binding Antibody Antibody Levels Antibody Production ... The human monoclonal antibodies of this invention can be used to separate bacterial toxins from biological samples by forming ... Human monoclonal antibodies specific to bacterial toxins can be used clinically for the prevention or treatment of disease. The ...
more infohttp://www.freepatentsonline.com/4689299.html

Bacterial luciferase Antibody, Biotin
		        
	Bacterial luciferase Antibody, Biotin

This antibody reacts with Bacteria, Luciferase samples. Supplied as 100 µL purified antibody (10 mg/ml) in 0.02M potassium ... Bacterial luciferase Polyclonal Antibody, Biotin conjugate from Invitrogen for Western Blot, Immunofluorescence, ... Cite Bacterial luciferase Polyclonal Antibody, Biotin. The following antibody was used in this experiment: Bacterial luciferase ... If an Invitrogen™ antibody doesnt perform as described on our website or datasheet, well replace the product at no cost to ...
more infohttps://www.thermofisher.com/antibody/product/Bacterial-Luciferase-Antibody-Polyclonal/PA1-26227

Epitope mapping of monoclonal and polyclonal antibodies using bacterial cell surface displayEpitope mapping of monoclonal and polyclonal antibodies using bacterial cell surface display

... Volk, Anna Luisa KTH, School of ... Here, we describe a high-resolution method for mapping epitopes of antibodies based on bacterial surface expression of antigen ... Antibody, Cell surface display, Epitope mapping, FACS, Gram-positive, S. carnosus National Category Cell and Molecular Biology ... 2014 (English)In: Monoclonal antibodies, Humana Press, 2014, 485-500 p.Chapter in book (Refereed) Abstract [en] The unique ...
more infohttp://kth.diva-portal.org/smash/record.jsf?pid=diva2:794868

cellular response to triacyl bacterial lipopeptide Antibodies | Invitrogen
                       
                       
    ...cellular response to triacyl bacterial lipopeptide Antibodies | Invitrogen ...

Antibodies for proteins involved in cellular response to triacyl bacterial lipopeptide pathways, according to their Panther/ ... Custom Antibody Service. Searching for an antibody we dont offer? We make custom antibodies for specific targets, species and ... Antibodies for proteins involved in cellular response to triacyl bacterial lipopeptide pathways; according to their Panther/ ... If an Invitrogen™ antibody doesnt perform as described on our website or datasheet,well replace the product at no cost to you ...
more infohttps://www.thermofisher.com/antibody/primary/panther/cellular%20response%20to%20triacyl%20bacterial%20lipopeptide

Immunobiology of Proteins & Peptides, VI: Human Immunodeficiency Virus, Antibody Immunoconjugates, Bacterial Vaccines,...Immunobiology of Proteins & Peptides, VI: Human Immunodeficiency Virus, Antibody Immunoconjugates, Bacterial Vaccines,...

Bacterial Vaccines, Immunomodulators available in on Powells.com, also read synopsis and reviews. ... Antibody-drug conjugates. Subject:. HIV antibodies.. Subject:. HIV -- immunology -- congresses.. Subject:. Antibodies, ... Bacterial vaccines.. Subject:. Aids. Subject:. Bacterial Vaccines -- immunology -- congresses.. Subject:. HIV. Subject:. ... Immunobiology of Proteins & Peptides, VI: Human Immunodeficiency Virus, Antibody Immunoconjugates, Bacterial Vaccines, ...
more infohttp://www.powells.com/book/immunobiology-of-proteins-peptides-vi-9780306440380

Antibody orientation at bacterial surfaces is related to invasive infection.Antibody orientation at bacterial surfaces is related to invasive infection.

... Nordenfelt, Pontus LU ; Waldemarson, Sofia LU ; ... the antibodies preferentially bind via Fab, facilitating opsonization and bacterial killing. IgG-poor environments represent ... the antibodies preferentially bind via Fab, facilitating opsonization and bacterial killing. IgG-poor environments represent ... the Fc-binding proteins were removed from the bacterial surface. Further... (More). Several of the most significant bacterial ...
more infohttps://lup.lub.lu.se/search/publication/3347327

BioLegend Bacterial Proteins And Toxins Antibodies ProductsBioLegend Bacterial Proteins And Toxins Antibodies Products

BioLegend offers a wide array of Bacterial Proteins And Toxins Antibodies Products. BioLegend develops and manufactures world- ...
more infohttps://www.biolegend.com/cell-biology-reagents-5334/bacterial-proteins-and-toxins-antibodies-13460/

Anti Escherichia coli 0157 Antibody, clone 1031  - Mouse Anti-Bacterial Monoclonal Antibody IF, IP - Buy Now! |AbgentAnti Escherichia coli 0157 Antibody, clone 1031 - Mouse Anti-Bacterial Monoclonal Antibody IF, IP - Buy Now! |Abgent

Mouse Anti-Bacterial Monoclonal Antibody validated in IF, IP (ABD12953), Abgent ... home , Products , Primary Antibodies , Anti Escherichia coli 0157 Antibody, clone 1031 Anti Escherichia coli 0157 Antibody, ... Submit your citation using an Abgent antibody to. [email protected], and receive a free "I Love Antibodies" mug. ... Submit your citation using an Abgent antibody to. [email protected], and receive a free "I Love Antibodies" mug. ...
more infohttp://www.abgent.com/products/ABD12953-Anti-Escherichia-coli-0157-Antibody-clone-1031-

Anti-Streptococcus pneumoniae Antibody, clone 128/390  - Mouse Anti-Bacterial Monoclonal Antibody IF, E - Buy Now! |AbgentAnti-Streptococcus pneumoniae Antibody, clone 128/390 - Mouse Anti-Bacterial Monoclonal Antibody IF, E - Buy Now! |Abgent

Mouse Anti-Bacterial Monoclonal Antibody validated in IF, E (ABD10778), Abgent ... Anti-Streptococcus pneumoniae Antibody, clone 128/390 Mouse Anti-Bacterial Monoclonal Antibody. ... Submit your citation using an Abgent antibody to. [email protected], and receive a free "I Love Antibodies" mug. ... Submit your citation using an Abgent antibody to. [email protected], and receive a free "I Love Antibodies" mug. ...
more infohttp://www.abgent.com/products/ABD10778-Anti-Streptococcus-pneumoniae-Antibody-clone-128390-

Antibody engineering: comparison of bacterial, yeast, insect and mammalian expression systems.  - PubMed - NCBIAntibody engineering: comparison of bacterial, yeast, insect and mammalian expression systems. - PubMed - NCBI

Antibody engineering: comparison of bacterial, yeast, insect and mammalian expression systems.. Verma R1, Boleti E, George AJ. ... Engineered antibody molecules, and their fragments, are being increasingly exploited as scientific and clinical tools. However ... There is no universal expression system, that can guarantee high yields of recombinant product, as every antibody-based ... In this review we describe the relative advantages and disadvantages of bacterial, yeast, insect and mammalian expression ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/9760222

Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer | SpringerLinkRetargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer | SpringerLink

Abstract The ever-increasing threat of multi-drug resistant bacterial infections has spurred renewed interest in alternative ... In this study, we examine whether these anti-Gal antibodies can be recruited and redirected to exert anti-bacterial activity. ... Oleksiewicz MB, Nagy G, Nagy E (2012) Anti-bacterial monoclonal antibodies: back to the future? Arch Biochem Biophys 526:124- ... Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer. ...
more infohttps://link.springer.com/article/10.1007%2Fs00109-015-1280-4

Exploring the impact of antibodies on the mechanics of bacterial fimbriaeExploring the impact of antibodies on the mechanics of bacterial fimbriae

... Mortezaei, Narges Umeå University, Faculty of ... Such evolutionary adaptation can thereby help in the bacterial adhesion process. Furthermore, we found that antibodies ... Open this publication in new window or tab ,,P-fimbriae in the presence of anti-PapA antibodies: new insight of antibodies ... 1. P-fimbriae in the presence of anti-PapA antibodies: new insight of antibodies action against pathogens. ...
more infohttp://umu.diva-portal.org/smash/record.jsf?pid=diva2:922844

Anti-Bacterial HCP AntibodiesAnti-Bacterial HCP Antibodies

... Antibodies against bacterial host cell proteins, including those from E. coli, L. lactis, P. ...
more infohttps://www.cygnustechnologies.com/browse/anti-bacterial-hcp-antibodies

Evidence for Antibody-Catalyzed Ozone Formation in Bacterial Killing and Inflammation | ScienceEvidence for Antibody-Catalyzed Ozone Formation in Bacterial Killing and Inflammation | Science

Evidence for Antibody-Catalyzed Ozone Formation in Bacterial Killing and Inflammation. By Paul Wentworth Jr., Jonathan E. ... Evidence for Antibody-Catalyzed Ozone Formation in Bacterial Killing and Inflammation. By Paul Wentworth Jr., Jonathan E. ... Evidence for Antibody-Catalyzed Ozone Formation in Bacterial Killing and Inflammation Message Subject. (Your Name) has ...
more infohttps://science.sciencemag.org/content/298/5601/2195/tab-article-info

Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer | SpringerLinkRetargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer | SpringerLink

Abstract The ever-increasing threat of multi-drug resistant bacterial infections has spurred renewed interest in alternative ... In this study, we examine whether these anti-Gal antibodies can be recruited and redirected to exert anti-bacterial activity. ... Oleksiewicz MB, Nagy G, Nagy E (2012) Anti-bacterial monoclonal antibodies: back to the future? Arch Biochem Biophys 526:124- ... Retargeting pre-existing human antibodies to a bacterial pathogen with an alpha-Gal conjugated aptamer. ...
more infohttps://link.springer.com/article/10.1007/s00109-015-1280-4

Ozone is Produced by Antibodies During Bacterial Killing and in 
InflammationOzone is Produced by Antibodies During Bacterial Killing and in Inflammation

Ozone is Produced by Antibodies During Bacterial Killing and in Inflammation, Say Scientists at The Scripps Research Institute ... this research opens up exciting possibilities for new antibody-. mediated therapies for conditions ranging from bacterial and ... The research article, Evidence for Antibody-Catalyzed Ozone Formation in Bacterial Killing and Inflammation is authored by ... Previously, antibodies were believed only to signal an immune response. This ability of antibodies to generate toxic compounds ...
more infohttps://mold-survivor.com/ozone_is_produced_by_antibodies.html

Limiting CDR-H3 Diversity Abrogates the Antibody Response to the Bacterial Polysaccharide α 1→3 Dextran | The Journal of...Limiting CDR-H3 Diversity Abrogates the Antibody Response to the Bacterial Polysaccharide α 1→3 Dextran | The Journal of...

Limiting CDR-H3 Diversity Abrogates the Antibody Response to the Bacterial Polysaccharide α 1→3 Dextran. Tamer I. Mahmoud, ... The mouse antibody response to infection with Cryptococcus neoformans: VH and VL usage in polysaccharide binding antibodies. J ... Limiting CDR-H3 Diversity Abrogates the Antibody Response to the Bacterial Polysaccharide α 1→3 Dextran ... Limiting CDR-H3 Diversity Abrogates the Antibody Response to the Bacterial Polysaccharide α 1→3 Dextran ...
more infohttp://www.jimmunol.org/content/187/2/879

Anti-Pseudomonas aeruginosa IgY antibodies promote bacterial opsonization and augment the phagocytic activity of...Anti-Pseudomonas aeruginosa IgY antibodies promote bacterial opsonization and augment the phagocytic activity of...

Anti-Pseudomonas aeruginosa IgY antibodies promote bacterial opsonization and augment the phagocytic activity of ... We have observed that egg yolk antibodies (IgY) harvested from White leghorn chickens that target P. aeruginosa opsonize the ... IgY may boost the innate immunity against Pseudomonas aeruginosa in the CF setting by facilitating a rapid and prompt bacterial ... pathogen and enhance the PMN-mediated respiratory burst and subsequent bacterial killing in vitro. The effects on PMN ...
more infohttps://publichealth.ku.dk/about-the-department/general/publications/publicationlistaam/?pure=en%2Fpublications%2Fantipseudomonas-aeruginosa-igy-antibodies-promote-bacterial-opsonization-and-augment-the-phagocytic-activity-of-polymorphonuclear-neutrophils

Toxins  | Free Full-Text | Recent Developments in Antibody-Based Assays for the Detection of Bacterial Toxins | NotesToxins | Free Full-Text | Recent Developments in Antibody-Based Assays for the Detection of Bacterial Toxins | Notes

Firstly, bacterial toxins will be categorized according to their antibody binding properties into low and high molecular weight ... the types of antibodies and new techniques for producing antibodies are discussed, including poly- and mono-clonal antibodies, ... for labeling antibodies and toxins or for readout techniques will be summarized. Fourthly, microscale analysis or minimized ... single-chain variable fragments (scFv), as well as heavy-chain and recombinant antibodies. Thirdly, the use of different ...
more infohttp://www.mdpi.com/2072-6651/6/4/1325/notes

Prevalence of antibodies to selected viral and bacterial pathogens in wild boar (Sus scrofa) in Campania Region, Italy. -...Prevalence of antibodies to selected viral and bacterial pathogens in wild boar (Sus scrofa) in Campania Region, Italy. -...

All sera tested seronegative for SVDV and CSFV antibodies. These results, recorded for the first time in Campania, support the ... Samples were tested for antibodies to Leptospira interrogan, Brucella spp., Salmonella spp., Aujeszky disease virus (ADV), ... Prevalence of antibodies to selected viral and bacterial pathogens in wild boar (Sus scrofa) in Campania Region, Italy.. @ ... article{Montagnaro2010PrevalenceOA, title={Prevalence of antibodies to selected viral and bacterial pathogens in wild boar (Sus ...
more infohttps://www.semanticscholar.org/paper/Prevalence-of-antibodies-to-selected-viral-and-in-Montagnaro-Sasso/3944c24a9f62f9a2e6d5c535f981182be36a9154

Background Low avidity of antibodies against viral, parasitic and bacterial agencies | Regulation of human neutrophil-mediated...Background Low avidity of antibodies against viral, parasitic and bacterial agencies | Regulation of human neutrophil-mediated...

Background Low avidity of antibodies against viral, parasitic and bacterial agencies. By Celina Scott in General Calcium ... Background Low avidity of antibodies against viral, parasitic and bacterial agencies continues to be employed for differential ... Ercalcidiol F2 GHR GSK1059615 GSK2118436A Itga11 Ixabepilone Kenpaullone LATS1 antibody LIMK2 antibody LIN28 antibody LY2484595 ... Regular ELISA for HPV6/11/16/18/31/33/45 antibodies was utilized [10,24-27], with adjustments [17-19] for HPV16 antibody ...
more infohttp://acmbcb.org/background-low-avidity-of-antibodies-against-viral-parasitic-and-bacterial-agencies/

Antibodies Produce Ozone During Bacterial Killing and Inflammation ArchivesAntibodies Produce Ozone During Bacterial Killing and Inflammation Archives

All posts in Antibodies Produce Ozone During Bacterial Killing and Inflammation. Antibodies Produce Ozone By ozoneresearch 24 ... Antibodies Produce Ozone Antibodies Produce Ozone During Bacterial Killing and Inflammation. By Jason Socrates Bardi ... Antibodies Produce Ozone During Bacterial Killing and Inflammation antibodies produce ozone, Ozone ... The research article, "Evidence for Antibody-Catalyzed Ozone Formation in Bacterial Killing and Inflammation" is authored by ...
more infohttps://medicalozone.info/category/antibodies-produce-ozone-during-bacterial-killing-and-inflammation/
  • The anti-GAS alphamer was shown to recruit anti-Gal antibodies to the streptococcal surface in an α-Gal-specific manner, elicit uptake and killing of the bacteria by human phagocytes, and slow growth of invasive GAS in human whole blood. (springer.com)
  • These studies provide a first in vitro proof of concept that alphamers have the potential to redirect pre-existing antibodies to bacteria in a specific manner and trigger an immediate antibacterial immune response. (springer.com)
  • La Jolla, CA. November 14, 2002-Professor Richard A. Lerner, M.D., Associate Professor Paul Wentworth, Jr., Ph.D., and a team of investigators at The Scripps Research Institute (TSRI) is reporting that antibodies can destroy bacteria, playing a hitherto unknown role in immune protection. (mold-survivor.com)
  • Now, Lerner, Wentworth and their colleagues have demonstrated that antibodies also have the ability to kill bacteria. (mold-survivor.com)
  • In the Science paper, the TSRI team reports the effective killing of E. coli bacteria through hydrogen peroxide production by antibodies specific for that bacteria. (mold-survivor.com)
  • The Scripps Research Institute (TSRI) President Richard A. Lerner, Associate Professor Paul Wentworth, Jr., Ph.D., and a team of investigators at TSRI is reporting that antibodies can destroy bacteria, playing a hitherto unknown role in immune protection. (medicalozone.info)
  • If an Invitrogenantibody doesn't perform as described on our website or datasheet, we'll replace the product at no cost to you, or provide you with a credit for a future purchase. (thermofisher.com)
  • We have observed that egg yolk antibodies (IgY) harvested from White leghorn chickens that target P. aeruginosa opsonize the pathogen and enhance the PMN-mediated respiratory burst and subsequent bacterial killing in vitro. (ku.dk)
  • Each antibody is crafted with care according to rigorous protocols for immunogen design and preparation, presentation to host animal, and high-affinity purification against the antigen. (abgent.com)
  • There is no 'universal' expression system, that can guarantee high yields of recombinant product, as every antibody-based molecule will pose its own problems in terms of expression. (nih.gov)
  • They have not yet demonstrated conclusively that what they found is ozone, but they are highly confident that ozone is what the antibodies are producing because no other known molecule has the same chemical signature. (mold-survivor.com)
  • All antibodies have the ability to produce hydrogen peroxide, the report adds, but they need to first have available a molecule known as 'singlet' oxygen-another highly reactive oxygen species-to use as a substrate. (mold-survivor.com)
  • Antibodies to B. burgdorferi were assessed by enzyme-linked immunosorbent assay (ELISA) and immunoblot analysis. (prohealth.com)
  • Regular ELISA for HPV6/11/16/18/31/33/45 antibodies was utilized [10,24- with adjustments [17-for HPV16 antibody avidity evaluation. (acmbcb.org)
  • Hence knowledge of the precise binding site (epitope) of antibodies on the target protein is one of the most important features for understanding its performance and determining its reliability in immunoassays. (diva-portal.org)
  • As a result the choice of system will depend on many factors, including the molecular species being expressed, the precise sequence of the individual antibody and the preferences of the individual investigator. (nih.gov)
  • 1. A continuous cell line which produces human anti-exotixin antibodies, comprising: a stable fused cell hybrid of a human peripheral blood lymphocyte immunized by a toxin, or an imunogenic fragment thereof, or a toxoid prepared from an exotoxin, or an immunogenic fragment thereof, and a mouse myeloma cell, in which the antibodies are capable of neutralizing exotoxin. (freepatentsonline.com)
  • 3. A continuous cell line which produces human anit-tetanus toxin antibodies, comprising: a stable fused cell hybrid of a tetanus toxin-immunized or toxoid-immunized human peripheral blood lymphocyte and a mouse myeloma cell, in which the anitbodies are capable of neutralizing tetanus toxin. (freepatentsonline.com)
  • The unique property of specific high-affinity binding to more or less any target of interest has made antibodies tremendously useful in numerous applications. (diva-portal.org)
  • The aims of this thesis are: to provide insight into the structural and biomechanical differences of fimbriae expressed by enterotoxigenic and uropathogenic Escherichia coli, and to investigate how fimbrial mechanics are affected in the presence of anti-fimbrial antibodies. (diva-portal.org)
  • We provide tools for path-breaking research by developing antibodies that detect a comprehensive library of novel and established targets. (abgent.com)
  • For new targets we consult with leading experts to accelerate development of antibodies that will propel state-of-the-art research in cellular health and disease. (abgent.com)
  • Crown antibodies pass additional stringent quality requirements, including extended control sets, uniform results against multiple biologically relevant cell lines and tissues, and function in multiple applications. (abgent.com)
  • During this right time, HPV is solved through T helper cell activation of cytotoxic T cells and B cells to create neutralizing IgG antibodies . (acmbcb.org)
  • In the throat, IgG was mostly bound to the bacterial surface via Fc, whereas in the blood IgG was mostly bound via fragment antigen-binding (Fab). (lu.se)
  • Certainly the most surprising result that Lerner, Wentworth, and their colleagues found was that antibodies appear to make ozone, which they detected through its chemical signature. (mold-survivor.com)
  • Alternatively, low-avidity antibodies have already been found (beyond your six months period) in chronic attacks, e.g., cytomegalovirus (CMV) and HPV16 [21- (acmbcb.org)
  • We used force measuring optical tweezers to characterize the force-extension responses of fimbriae in the absence and presence of antibodies. (diva-portal.org)
  • This ability of antibodies to generate toxic compounds may also link them to a number of inflammatory diseases, such as atherosclerosis, lupus, multiple sclerosis, and rheumatoid arthritis. (mold-survivor.com)
  • Proper validation with controls and optimization ensure the quality of the antibody, and establish confidence in your results. (abgent.com)
  • Immunoblots of the 3 controls with increased antibodies showed only isolated 41-kd reactivity. (prohealth.com)
  • Both seroprevalence and antibody optical density index exhibited 1-year cycles, indicating that disease outbreaks were seasonal and suggesting that long-lasting antibody protection was transient. (nih.gov)
  • Bispecific antibody-targeted minicells, packaged with the chemotherapeutic paclitaxel, were shown to be safe in patients with advanced solid tumors with modest clinical efficacy observed. (edu.au)