Autoantibodies directed against various nuclear antigens including DNA, RNA, histones, acidic nuclear proteins, or complexes of these molecular elements. Antinuclear antibodies are found in systemic autoimmune diseases including systemic lupus erythematosus, Sjogren's syndrome, scleroderma, polymyositis, and mixed connective tissue disease.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Immunoglobulins produced in response to VIRAL ANTIGENS.
Antibodies that react with self-antigens (AUTOANTIGENS) of the organism that produced them.
Antibodies produced by a single clone of cells.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
A chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.
Antibodies that reduce or abolish some biological activity of a soluble antigen or infectious agent, usually a virus.
A heterogeneous group of disorders, some hereditary, others acquired, characterized by abnormal structure or function of one or more of the elements of connective tissue, i.e., collagen, elastin, or the mucopolysaccharides.
Antibodies found in adult RHEUMATOID ARTHRITIS patients that are directed against GAMMA-CHAIN IMMUNOGLOBULINS.
A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
Antibodies which react with the individual structural determinants (idiotopes) on the variable region of other antibodies.
Disorders that are characterized by the production of antibodies that react with host tissues or immune effector cells that are autoreactive to endogenous peptides.
A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes.
A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
Local surface sites on antibodies which react with antigen determinant sites on antigens (EPITOPES.) They are formed from parts of the variable regions of FAB FRAGMENTS.
A chronic multi-system disorder of CONNECTIVE TISSUE. It is characterized by SCLEROSIS in the SKIN, the LUNGS, the HEART, the GASTROINTESTINAL TRACT, the KIDNEYS, and the MUSCULOSKELETAL SYSTEM. Other important features include diseased small BLOOD VESSELS and AUTOANTIBODIES. The disorder is named for its most prominent feature (hard skin), and classified into subsets by the extent of skin thickening: LIMITED SCLERODERMA and DIFFUSE SCLERODERMA.
Antibodies reactive with HIV ANTIGENS.
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
Sites on an antigen that interact with specific antibodies.
Immunoglobulins induced by antigens specific for tumors other than the normally occurring HISTOCOMPATIBILITY ANTIGENS.
Immunoglobulins produced in a response to PROTOZOAN ANTIGENS.
Disorders of connective tissue, especially the joints and related structures, characterized by inflammation, degeneration, or metabolic derangement.
Chronic inflammatory and autoimmune disease in which the salivary and lacrimal glands undergo progressive destruction by lymphocytes and plasma cells resulting in decreased production of saliva and tears. The primary form, often called sicca syndrome, involves both KERATOCONJUNCTIVITIS SICCA and XEROSTOMIA. The secondary form includes, in addition, the presence of a connective tissue disease, usually rheumatoid arthritis.
A chronic self-perpetuating hepatocellular INFLAMMATION of unknown cause, usually with HYPERGAMMAGLOBULINEMIA and serum AUTOANTIBODIES.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Endogenous tissue constituents that have the ability to interact with AUTOANTIBODIES and cause an immune response.
An idiopathic vascular disorder characterized by bilateral Raynaud phenomenon, the abrupt onset of digital paleness or CYANOSIS in response to cold exposure or stress.
The complex formed by the binding of antigen and antibody molecules. The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES.
Arthritis of children, with onset before 16 years of age. The terms juvenile rheumatoid arthritis (JRA) and juvenile idiopathic arthritis (JIA) refer to classification systems for chronic arthritis in children. Only one subtype of juvenile arthritis (polyarticular-onset, rheumatoid factor-positive) clinically resembles adult rheumatoid arthritis and is considered its childhood equivalent.
Inflammation of the iris characterized by circumcorneal injection, aqueous flare, keratotic precipitates, and constricted and sluggish pupil along with discoloration of the iris.
Autoantibodies directed against phospholipids. These antibodies are characteristically found in patients with systemic lupus erythematosus (LUPUS ERYTHEMATOSUS, SYSTEMIC;), ANTIPHOSPHOLIPID SYNDROME; related autoimmune diseases, some non-autoimmune diseases, and also in healthy individuals.
A syndrome with overlapping clinical features of systemic lupus erythematosus, scleroderma, polymyositis, and Raynaud's phenomenon. The disease is differentially characterized by high serum titers of antibodies to ribonuclease-sensitive extractable (saline soluble) nuclear antigen and a "speckled" epidermal nuclear staining pattern on direct immunofluorescence.
Autoantibodies directed against cytoplasmic constituents of POLYMORPHONUCLEAR LEUKOCYTES and/or MONOCYTES. They are used as specific markers for GRANULOMATOSIS WITH POLYANGIITIS and other diseases, though their pathophysiological role is not clear. ANCA are routinely detected by indirect immunofluorescence with three different patterns: c-ANCA (cytoplasmic), p-ANCA (perinuclear), and atypical ANCA.
Represents 15-20% of the human serum immunoglobulins, mostly as the 4-chain polymer in humans or dimer in other mammals. Secretory IgA (IMMUNOGLOBULIN A, SECRETORY) is the main immunoglobulin in secretions.
Immunoglobulins produced in a response to FUNGAL ANTIGENS.
A term used to describe a variety of localized asymmetrical SKIN thickening that is similar to those of SYSTEMIC SCLERODERMA but without the disease features in the multiple internal organs and BLOOD VESSELS. Lesions may be characterized as patches or plaques (morphea), bands (linear), or nodules.
FIBROSIS of the hepatic parenchyma due to obstruction of BILE flow (CHOLESTASIS) in the intrahepatic or extrahepatic bile ducts (BILE DUCTS, INTRAHEPATIC; BILE DUCTS, EXTRAHEPATIC). Primary biliary cirrhosis involves the destruction of small intra-hepatic bile ducts and bile secretion. Secondary biliary cirrhosis is produced by prolonged obstruction of large intrahepatic or extrahepatic bile ducts from a variety of causes.
Glomerulonephritis associated with autoimmune disease SYSTEMIC LUPUS ERYTHEMATOSUS. Lupus nephritis is histologically classified into 6 classes: class I - normal glomeruli, class II - pure mesangial alterations, class III - focal segmental glomerulonephritis, class IV - diffuse glomerulonephritis, class V - diffuse membranous glomerulonephritis, and class VI - advanced sclerosing glomerulonephritis (The World Health Organization classification 1982).
The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).
The processes triggered by interactions of ANTIBODIES with their ANTIGENS.
Antibodies, often monoclonal, in which the two antigen-binding sites are specific for separate ANTIGENIC DETERMINANTS. They are artificial antibodies produced by chemical crosslinking, fusion of HYBRIDOMA cells, or by molecular genetic techniques. They function as the main mediators of targeted cellular cytotoxicity and have been shown to be efficient in the targeting of drugs, toxins, radiolabeled haptens, and effector cells to diseased tissue, primarily tumors.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Antibodies which elicit IMMUNOPRECIPITATION when combined with antigen.
Multi-subunit proteins which function in IMMUNITY. They are produced by B LYMPHOCYTES from the IMMUNOGLOBULIN GENES. They are comprised of two heavy (IMMUNOGLOBULIN HEAVY CHAINS) and two light chains (IMMUNOGLOBULIN LIGHT CHAINS) with additional ancillary polypeptide chains depending on their isoforms. The variety of isoforms include monomeric or polymeric forms, and transmembrane forms (B-CELL ANTIGEN RECEPTORS) or secreted forms (ANTIBODIES). They are divided by the amino acid sequence of their heavy chains into five classes (IMMUNOGLOBULIN A; IMMUNOGLOBULIN D; IMMUNOGLOBULIN E; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M) and various subclasses.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Inflammation of any part of the KIDNEY.
A form of antibodies consisting only of the variable regions of the heavy and light chains (FV FRAGMENTS), connected by a small linker peptide. They are less immunogenic than complete immunoglobulin and thus have potential therapeutic use.
The protein components that constitute the common core of small nuclear ribonucleoprotein particles. These proteins are commonly referred as Sm nuclear antigens due to their antigenic nature.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Antibodies that inhibit the reaction between ANTIGEN and other antibodies or sensitized T-LYMPHOCYTES (e.g., antibodies of the IMMUNOGLOBULIN G class that compete with IGE antibodies for antigen, thereby blocking an allergic response). Blocking antibodies that bind tumors and prevent destruction of tumor cells by CYTOTOXIC T-LYMPHOCYTES have also been called enhancing antibodies. (Rosen et al., Dictionary of Immunology, 1989)
Inflammation of a serous membrane.
A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.
Process whereby the immune system reacts against the body's own tissues. Autoimmunity may produce or be caused by AUTOIMMUNE DISEASES.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Substances that are recognized by the immune system and induce an immune reaction.
Univalent antigen-binding fragments composed of one entire IMMUNOGLOBULIN LIGHT CHAIN and the amino terminal end of one of the IMMUNOGLOBULIN HEAVY CHAINS from the hinge region, linked to each other by disulfide bonds. Fab contains the IMMUNOGLOBULIN VARIABLE REGIONS, which are part of the antigen-binding site, and the first IMMUNOGLOBULIN CONSTANT REGIONS. This fragment can be obtained by digestion of immunoglobulins with the proteolytic enzyme PAPAIN.
Inflammation of a muscle or muscle tissue.
Immunologically detectable substances found in the CELL NUCLEUS.
Antiphospholipid antibodies found in association with systemic lupus erythematosus (LUPUS ERYTHEMATOSUS, SYSTEMIC;), ANTIPHOSPHOLIPID SYNDROME; and in a variety of other diseases as well as in healthy individuals. The antibodies are detected by solid-phase IMMUNOASSAY employing the purified phospholipid antigen CARDIOLIPIN.
Antibodies elicited in a different species from which the antigen originated. These antibodies are directed against a wide variety of interspecies-specific antigens, the best known of which are Forssman, Hanganutziu-Deicher (H-D), and Paul-Bunnell (P-B). Incidence of antibodies to these antigens--i.e., the phenomenon of heterophile antibody response--is useful in the serodiagnosis, pathogenesis, and prognosis of infection and latent infectious states as well as in cancer classification.
Diagnostic procedures involving immunoglobulin reactions.
Antibodies that can catalyze a wide variety of chemical reactions. They are characterized by high substrate specificity and share many mechanistic features with enzymes.
Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
A form of lupus erythematosus in which the skin may be the only organ involved or in which skin involvement precedes the spread into other body systems. It has been classified into three forms - acute (= LUPUS ERYTHEMATOSUS, SYSTEMIC with skin lesions), subacute, and chronic (= LUPUS ERYTHEMATOSUS, DISCOID).
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
A chronic systemic disease, primarily of the joints, marked by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Etiology is unknown, but autoimmune mechanisms have been implicated.
Serum glycoproteins participating in the host defense mechanism of COMPLEMENT ACTIVATION that creates the COMPLEMENT MEMBRANE ATTACK COMPLEX. Included are glycoproteins in the various pathways of complement activation (CLASSICAL COMPLEMENT PATHWAY; ALTERNATIVE COMPLEMENT PATHWAY; and LECTIN COMPLEMENT PATHWAY).
Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.
Antibodies from non-human species whose protein sequences have been modified to make them nearly identical with human antibodies. If the constant region and part of the variable region are replaced, they are called humanized. If only the constant region is modified they are called chimeric. INN names for humanized antibodies end in -zumab.
A form of cutaneous tuberculosis. It is seen predominantly in women and typically involves the NASAL MUCOSA; BUCCAL MUCOSA; and conjunctival mucosa.
A genus of parasitic protozoans found in the digestive tract of invertebrates, especially insects. Organisms of this genus have an amastigote and choanomastigote stage in their life cycle.
Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell.
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
A chronic form of cutaneous lupus erythematosus (LUPUS ERYTHEMATOSUS, CUTANEOUS) in which the skin lesions mimic those of the systemic form but in which systemic signs are rare. It is characterized by the presence of discoid skin plaques showing varying degrees of edema, erythema, scaliness, follicular plugging, and skin atrophy. Lesions are surrounded by an elevated erythematous border. The condition typically involves the face and scalp, but widespread dissemination may occur.
A glycoprotein that is important in the activation of CLASSICAL COMPLEMENT PATHWAY. C4 is cleaved by the activated COMPLEMENT C1S into COMPLEMENT C4A and COMPLEMENT C4B.
Methods used for studying the interactions of antibodies with specific regions of protein antigens. Important applications of epitope mapping are found within the area of immunochemistry.
Inflammation of the renal glomeruli (KIDNEY GLOMERULUS) that can be classified by the type of glomerular injuries including antibody deposition, complement activation, cellular proliferation, and glomerulosclerosis. These structural and functional abnormalities usually lead to HEMATURIA; PROTEINURIA; HYPERTENSION; and RENAL INSUFFICIENCY.
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
Small RNAs found in the cytoplasm usually complexed with proteins in scRNPs (RIBONUCLEOPROTEINS, SMALL CYTOPLASMIC).
Established cell cultures that have the potential to propagate indefinitely.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
Positive test results in subjects who do not possess the attribute for which the test is conducted. The labeling of healthy persons as diseased when screening in the detection of disease. (Last, A Dictionary of Epidemiology, 2d ed)
Elements of limited time intervals, contributing to particular results or situations.
Complexes of RNA-binding proteins with ribonucleic acids (RNA).
Substances elaborated by bacteria that have antigenic activity.
An encapsulated lymphatic organ through which venous blood filters.
A subacute or chronic inflammatory disease of muscle and skin, marked by proximal muscle weakness and a characteristic skin rash. The illness occurs with approximately equal frequency in children and adults. The skin lesions usually take the form of a purplish rash (or less often an exfoliative dermatitis) involving the nose, cheeks, forehead, upper trunk, and arms. The disease is associated with a complement mediated intramuscular microangiopathy, leading to loss of capillaries, muscle ischemia, muscle-fiber necrosis, and perifascicular atrophy. The childhood form of this disease tends to evolve into a systemic vasculitis. Dermatomyositis may occur in association with malignant neoplasms. (From Adams et al., Principles of Neurology, 6th ed, pp1405-6)
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
Acidic phospholipids composed of two molecules of phosphatidic acid covalently linked to a molecule of glycerol. They occur primarily in mitochondrial inner membranes and in bacterial plasma membranes. They are the main antigenic components of the Wassermann-type antigen that is used in nontreponemal SYPHILIS SERODIAGNOSIS.
Transfer of immunity from immunized to non-immune host by administration of serum antibodies, or transplantation of lymphocytes (ADOPTIVE TRANSFER).
The presence of antibodies directed against phospholipids (ANTIBODIES, ANTIPHOSPHOLIPID). The condition is associated with a variety of diseases, notably systemic lupus erythematosus and other connective tissue diseases, thrombopenia, and arterial or venous thromboses. In pregnancy it can cause abortion. Of the phospholipids, the cardiolipins show markedly elevated levels of anticardiolipin antibodies (ANTIBODIES, ANTICARDIOLIPIN). Present also are high levels of lupus anticoagulant (LUPUS COAGULATION INHIBITOR).
Proteins prepared by recombinant DNA technology.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Inflammation of any one of the blood vessels, including the ARTERIES; VEINS; and rest of the vasculature system in the body.
Serum globulins that migrate to the gamma region (most positively charged) upon ELECTROPHORESIS. At one time, gamma-globulins came to be used as a synonym for immunoglobulins since most immunoglobulins are gamma globulins and conversely most gamma globulins are immunoglobulins. But since some immunoglobulins exhibit an alpha or beta electrophoretic mobility, that usage is in decline.
A broad-specificity HLA-DR antigen that is associated with HLA-DRB1 CHAINS encoded by DRB1*11 and DRB1*12 alleles.
Partial immunoglobulin molecules resulting from selective cleavage by proteolytic enzymes or generated through PROTEIN ENGINEERING techniques.
The sum of the weight of all the atoms in a molecule.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
A cluster of convoluted capillaries beginning at each nephric tubule in the kidney and held together by connective tissue.
Substances elaborated by viruses that have antigenic activity.
Highly conserved nuclear RNA-protein complexes that function in RNA processing in the nucleus, including pre-mRNA splicing and pre-mRNA 3'-end processing in the nucleoplasm, and pre-rRNA processing in the nucleolus (see RIBONUCLEOPROTEINS, SMALL NUCLEOLAR).
Glands that secrete SALIVA in the MOUTH. There are three pairs of salivary glands (PAROTID GLAND; SUBLINGUAL GLAND; SUBMANDIBULAR GLAND).
Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Group of diseases mediated by the deposition of large soluble complexes of antigen and antibody with resultant damage to tissue. Besides SERUM SICKNESS and the ARTHUS REACTION, evidence supports a pathogenic role for immune complexes in many other IMMUNE SYSTEM DISEASES including GLOMERULONEPHRITIS, systemic lupus erythematosus (LUPUS ERYTHEMATOSUS, SYSTEMIC) and POLYARTERITIS NODOSA.
Antigens determined by leukocyte loci found on chromosome 6, the major histocompatibility loci in humans. They are polypeptides or glycoproteins found on most nucleated cells and platelets, determine tissue types for transplantation, and are associated with certain diseases.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
A glycoprotein that is central in both the classical and the alternative pathway of COMPLEMENT ACTIVATION. C3 can be cleaved into COMPLEMENT C3A and COMPLEMENT C3B, spontaneously at low level or by C3 CONVERTASE at high level. The smaller fragment C3a is an ANAPHYLATOXIN and mediator of local inflammatory process. The larger fragment C3b binds with C3 convertase to form C5 convertase.
Within most types of eukaryotic CELL NUCLEUS, a distinct region, not delimited by a membrane, in which some species of rRNA (RNA, RIBOSOMAL) are synthesized and assembled into ribonucleoprotein subunits of ribosomes. In the nucleolus rRNA is transcribed from a nucleolar organizer, i.e., a group of tandemly repeated chromosomal genes which encode rRNA and which are transcribed by RNA polymerase I. (Singleton & Sainsbury, Dictionary of Microbiology & Molecular Biology, 2d ed)
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
A class Ia antiarrhythmic drug that is structurally-related to PROCAINE.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed)
Measurement of rate of settling of erythrocytes in anticoagulated blood.
Serologic tests in which a known quantity of antigen is added to the serum prior to the addition of a red cell suspension. Reaction result is expressed as the smallest amount of antigen which causes complete inhibition of hemagglutination.
That region of the immunoglobulin molecule that varies in its amino acid sequence and composition, and comprises the binding site for a specific antigen. It is located at the N-terminus of the Fab fragment of the immunoglobulin. It includes hypervariable regions (COMPLEMENTARITY DETERMINING REGIONS) and framework regions.
EPIDEMIOLOGIC STUDIES based on the detection through serological testing of characteristic change in the serum level of specific ANTIBODIES. Latent subclinical infections and carrier states can thus be detected in addition to clinically overt cases.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.
INFLAMMATION of salivary tissue (SALIVARY GLANDS), usually due to INFECTION or injuries.
An excess of GAMMA-GLOBULINS in the serum due to chronic infections or PARAPROTEINEMIAS.
Unique genetically-controlled determinants present on ANTIBODIES whose specificity is limited to a single group of proteins (e.g., another antibody molecule or an individual myeloma protein). The idiotype appears to represent the antigenicity of the antigen-binding site of the antibody and to be genetically codetermined with it. The idiotypic determinants have been precisely located to the IMMUNOGLOBULIN VARIABLE REGION of both immunoglobin polypeptide chains.
Techniques used to demonstrate or measure an immune response, and to identify or measure antigens using antibodies.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
The acquired form of infection by Toxoplasma gondii in animals and man.
Mouse strains constructed to possess identical genotypes except for a difference at a single gene locus.
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Techniques for removal by adsorption and subsequent elution of a specific antibody or antigen using an immunosorbent containing the homologous antigen or antibody.
Small antigenic determinants capable of eliciting an immune response only when coupled to a carrier. Haptens bind to antibodies but by themselves cannot elicit an antibody response.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
A mouse substrain that is genetically predisposed to the development of systemic lupus erythematosus-like syndrome, which has been found to be clinically similar to the human disease. It has been determined that this mouse strain carries a mutation in the fas gene. Also, the MRL/lpr is a useful model to study behavioral and cognitive deficits found in autoimmune diseases and the efficacy of immunosuppressive agents.
Enlargement of the spleen.
The phenomenon of immense variability characteristic of ANTIBODIES. It enables the IMMUNE SYSTEM to react specifically against the essentially unlimited kinds of ANTIGENS it encounters. Antibody diversity is accounted for by three main theories: (1) the Germ Line Theory, which holds that each antibody-producing cell has genes coding for all possible antibody specificities, but expresses only the one stimulated by antigen; (2) the Somatic Mutation Theory, which holds that antibody-producing cells contain only a few genes, which produce antibody diversity by mutation; and (3) the Gene Rearrangement Theory, which holds that antibody diversity is generated by the rearrangement of IMMUNOGLOBULIN VARIABLE REGION gene segments during the differentiation of the ANTIBODY-PRODUCING CELLS.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
A collection of cloned peptides, or chemically synthesized peptides, frequently consisting of all possible combinations of amino acids making up an n-amino acid peptide.
Enzymes which catalyze the hydrolases of ester bonds within DNA. EC 3.1.-.
Removal and pathologic examination of specimens in the form of small pieces of tissue from the living body.
Antibodies to the HEPATITIS C ANTIGENS including antibodies to envelope, core, and non-structural proteins.
Proteins conjugated with nucleic acids.
Antibodies from an individual that react with ISOANTIGENS of another individual of the same species.
The classes of immunoglobulins found in any species of animal. In man there are nine classes that migrate in five different groups in electrophoresis; they each consist of two light and two heavy protein chains, and each group has distinguishing structural and functional properties.
A chronic inflammatory condition affecting the axial joints, such as the SACROILIAC JOINT and other intervertebral or costovertebral joints. It occurs predominantly in young males and is characterized by pain and stiffness of joints (ANKYLOSIS) with inflammation at tendon insertions.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
A highly vascularized endocrine gland consisting of two lobes joined by a thin band of tissue with one lobe on each side of the TRACHEA. It secretes THYROID HORMONES from the follicular cells and CALCITONIN from the parafollicular cells thereby regulating METABOLISM and CALCIUM level in blood, respectively.
Small chromosomal proteins (approx 12-20 kD) possessing an open, unfolded structure and attached to the DNA in cell nuclei by ionic linkages. Classification into the various types (designated histone I, histone II, etc.) is based on the relative amounts of arginine and lysine in each.
Antibodies obtained from a single clone of cells grown in mice or rats.
Studies used to test etiologic hypotheses in which inferences about an exposure to putative causal factors are derived from data relating to characteristics of persons under study or to events or experiences in their past. The essential feature is that some of the persons under study have the disease or outcome of interest and their characteristics are compared with those of unaffected persons.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
Administration of vaccines to stimulate the host's immune response. This includes any preparation intended for active immunological prophylaxis.
Diseases which have one or more of the following characteristics: they are permanent, leave residual disability, are caused by nonreversible pathological alteration, require special training of the patient for rehabilitation, or may be expected to require a long period of supervision, observation, or care. (Dictionary of Health Services Management, 2d ed)
Antibodies to the HEPATITIS B ANTIGENS, including antibodies to the surface (Australia) and core of the Dane particle and those to the "e" antigens.
The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.
Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
Resistance to a disease-causing agent induced by the introduction of maternal immunity into the fetus by transplacental transfer or into the neonate through colostrum and milk.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
Antibodies specific to INSULIN.
Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.
Studies which start with the identification of persons with a disease of interest and a control (comparison, referent) group without the disease. The relationship of an attribute to the disease is examined by comparing diseased and non-diseased persons with regard to the frequency or levels of the attribute in each group.
Drugs that are used to treat RHEUMATOID ARTHRITIS.
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
The clear constricted portion of the chromosome at which the chromatids are joined and by which the chromosome is attached to the spindle during cell division.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.
A single chain of deoxyribonucleotides that occurs in some bacteria and viruses. It usually exists as a covalently closed circle.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Any part or derivative of any protozoan that elicits immunity; malaria (Plasmodium) and trypanosome antigens are presently the most frequently encountered.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
3-Mercapto-D-valine. The most characteristic degradation product of the penicillin antibiotics. It is used as an antirheumatic and as a chelating agent in Wilson's disease.
The phenomenon of antibody-mediated target cell destruction by non-sensitized effector cells. The identity of the target cell varies, but it must possess surface IMMUNOGLOBULIN G whose Fc portion is intact. The effector cell is a "killer" cell possessing Fc receptors. It may be a lymphocyte lacking conventional B- or T-cell markers, or a monocyte, macrophage, or polynuclear leukocyte, depending on the identity of the target cell. The reaction is complement-independent.
A characteristic symptom complex.
The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.
Observation of a population for a sufficient number of persons over a sufficient number of years to generate incidence or mortality rates subsequent to the selection of the study group.
An immunoglobulin fragment composed of one variable domain from an IMMUNOGLOBULIN HEAVY CHAIN or IMMUNOGLOBULIN LIGHT CHAIN.
Polysaccharides found in bacteria and in capsules thereof.
The rate dynamics in chemical or physical systems.
A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Unstable isotopes of iodine that decay or disintegrate emitting radiation. I atoms with atomic weights 117-139, except I 127, are radioactive iodine isotopes.
Studies in which individuals or populations are followed to assess the outcome of exposures, procedures, or effects of a characteristic, e.g., occurrence of disease.
The total number of cases of a given disease in a specified population at a designated time. It is differentiated from INCIDENCE, which refers to the number of new cases in the population at a given time.
Agents that suppress immune function by one of several mechanisms of action. Classical cytotoxic immunosuppressants act by inhibiting DNA synthesis. Others may act through activation of T-CELLS or by inhibiting the activation of HELPER CELLS. While immunosuppression has been brought about in the past primarily to prevent rejection of transplanted organs, new applications involving mediation of the effects of INTERLEUKINS and other CYTOKINES are emerging.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Suspensions of attenuated or killed bacteria administered for the prevention or treatment of infectious bacterial disease.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Field of chemistry that pertains to immunological phenomena and the study of chemical reactions related to antigen stimulation of tissues. It includes physicochemical interactions between antigens and antibodies.
Layers of protein which surround the capsid in animal viruses with tubular nucleocapsids. The envelope consists of an inner layer of lipids and virus specified proteins also called membrane or matrix proteins. The outer layer consists of one or more types of morphological subunits called peplomers which project from the viral envelope; this layer always consists of glycoproteins.

Autoantibodies to RNA polymerases recognize multiple subunits and demonstrate cross-reactivity with RNA polymerase complexes. (1/2145)

OBJECTIVE: To determine the subunit specificity of autoantibody directed to RNA polymerases (RNAP) I, II, and III, which is one of the major autoantibody responses in patients with systemic sclerosis (SSc). METHODS: Thirty-two SSc sera with anti-RNAP antibodies (23 with anti-RNAP I/III, 5 with anti-RNAP I/III and II, and 4 with anti-RNAP II alone) were analyzed by immunoblotting using affinity-purified RNAP and by immunoprecipitation using 35S-labeled cell extracts in which RNAP complexes were dissociated. Antibodies bound to individual RNAP subunits were eluted from preparative immunoblots and were further analyzed by immunoblotting and immunoprecipitation. RESULTS: At least 15 different proteins were bound by antibodies in anti-RNAP-positive SSc sera in various combinations. All 9 sera immunoprecipitating RNAP II and all 28 sera immunoprecipitating RNAP I/III recognized the large subunit proteins of RNAP II and III, respectively. Reactivity to RNAP I large subunits was strongly associated with bright nucleolar staining by indirect immunofluorescence. Affinity-purified antibodies that recognized a 62-kd subunit protein cross-reacted with a 43-kd subunit protein and immunoprecipitated both RNAP I and RNAP III. Antibodies that recognized a 21-kd subunit protein obtained from sera that were positive for anti-RNAP I/III and II antibodies immunoprecipitated both RNAP II and RNAP III. CONCLUSION: Anti-RNAP antibodies recognize multiple subunits of RNAP I, II, and III. Moreover, the results of this study provide the first direct evidence that antibodies that recognize shared subunits of human RNAPs or epitopes present on different human RNAP subunits are responsible for the recognition of multiple RNAPs by SSc sera.  (+info)

Estrogen enhancement of anti-double-stranded DNA antibody and immunoglobulin G production in peripheral blood mononuclear cells from patients with systemic lupus erythematosus. (2/2145)

OBJECTIVE: To study the in vitro effect of estrogen on IgG anti-double-stranded DNA (anti-dsDNA) antibody and total IgG production in peripheral blood mononuclear cells (PBMC) from patients with systemic lupus erythematosus (SLE), in order to elucidate its regulatory role in SLE. METHODS: PBMC from SLE patients and normal donors were cultured with 17beta-estradiol (E2). IgG anti-dsDNA antibodies, total IgG, and cytokine activity in the culture supernatants were measured by enzyme-linked immunosorbent assay. RESULTS: E2 enhanced production of IgG anti-dsDNA antibodies as well as total IgG in PBMC from SLE patients. Anti-dsDNA production in patients with inactive disease was less responsive to E2 than that in patients with active disease. E2 also enhanced total IgG, but not anti-dsDNA, production in the PBMC of normal donors. Antibody production was increased by E2 to a lesser extent in patients' B cells than in their PBMC. Anti-interleukin-10 (anti-IL-10) antibodies partially blocked the E2-induced increase in antibody production in patients' PBMC, but anti-IL-10 had no effect on B cells. E2 increased IL-10 production by patients' monocytes. Exogenous IL-10 acted additively with E2 in increasing antibody production in patients' B cells. CONCLUSION: These results suggest that E2 may polyclonally increase the production of IgG, including IgG anti-dsDNA, in SLE patients' PBMC by enhancing B cell activity and by promoting IL-10 production in monocytes. These findings support the involvement of E2 in the pathogenesis of SLE.  (+info)

Up-regulation of glomerular extracellular matrix and transforming growth factor-beta expression in RF/J mice. (3/2145)

BACKGROUND: RF/J mice were first reported as a murine model of spontaneous glomerulosclerosis by Gude and Lupton in 1960, but the precise histologic characteristics and immunopathological background of this mouse have not been investigated further. METHODS: Measurements of serum levels of immunoglobulins, anti-single strand DNA (anti-ss-DNA) antibody, complement (C3), and circulating immune complex (IC) were performed. Analyses of glomerular histological and immunopathological lesions in association with the detection of mRNA expression of collagen IV, TGF-beta, matrix protein turnover related enzymes, matrix metalloproteinase-2 (MMP-2), tissue inhibitor of metalloproteinase-2 (TIMP-2) and platelet-derived growth factor (PDGF) were also performed in young (10-week-old) and elderly (60-week-old) RF/J mice with age-matched BALB/C mice as the controls. RESULTS: High levels of serum IgA and IgG from as early as 20 weeks of age were noted in the RF/J mice. Serum anti-ss-DNA antibody of aged RF/J mice increased up to 23% of that of aged MRL-lpr/lpr mice, and serum C3 concentration significantly decreased with age, reaching lower levels than that of BALB/c mice. IgA-IC levels were significantly high compared to BALB/C mice both in the early and late stages of life, whereas IgG-IC levels were high only in mice younger than 20 weeks. Semiquantitative and quantitative analyzes of renal histopathological findings revealed significantly marked and age-related mesangial matrix expansion in RF/J mice, with increasing frequency of global glomerular sclerosis and tubulointerstitial damage. On the other hand, although precise measurements of glomerular cell numbers also showed an apparent augmentation in both young and old RF/J mice compared to BALB/C mice, glomerular cellularity decreased with age in RF/J mice. Immunohistochemical study revealed massive immunoglobulin deposition from a young age in association with significantly higher accumulation of matrix proteins, such as types I and IV collagen and laminin from the early stage of life. In addition, in these glomeruli, transforming growth factor-beta1 (TGF-beta1) was highly expressed both in young and old mice. The mRNA expression of MMP-2 was up-regulated only in the early stage of life. Although PDGF mRNA of RF/J mice was significantly up-regulated in the early stage of life, the differences between the mice disappeared in the late stage of life. CONCLUSIONS: These findings suggest that in RF/J mice, an immunopathological background inducing high serum immunoglobulin and IC levels from the early stage of life is closely related to mesangioproliferative glomerular lesions mediated by PDGF, and that development of massive extracellular matrix accumulation in glomeruli was induced by up-regulated expression of TGF-beta with inappropriate regulation of protein turnover-related enzyme production.  (+info)

Cryoglobulinaemia and rheumatic manifestations in patients with hepatitis C virus infection. (4/2145)

OBJECTIVES: To investigate the association of cryoglobulinaemia and rheumatic manifestations in Korean patients with hepatitis C virus (HCV) infection. METHODS: Forty nine Korean patients with HCV infection were recruited. The prevalence, concentration, and type of cryoglobulin (by immunofixation), rheumatoid factor (RF), antinuclear antibody (ANA), and various rheumatological symptoms were investigated and HCV genotype was determined by polymerase chain reaction with genotype specific primer. RESULTS: The prevalence of cryoglobulin was 59% in Korean HCV patients and the concentration of cryoglobulin was 9.8 (7.9) g/l (mean (SD)). The type of cryoglobulinaemia was identified in 23 (80%) of 29 HCV patients with cryoglobulinaemia and they were all type III. There were no differences in age, sex, history of operation and transfusion, proportion of liver cirrhosis between the patients with cryoglobulinaemia and those without cryoglobulinaemia. The frequencies of RF and ANA were 14% and 3.4% respectively in HCV patients with cryoglobulinaemia. There was no difference in HCV genotype between the patients with cryoglobulinaemia and those without cryoglobulinaemia. Clinical features of HCV patients were as follows: arthralgia/arthritis (35%), cutaneous manifestation (37%), Raynaud's phenomenon (8%), paresthesia (44%), dry eyes (22%), dry mouth (10%), oral ulcer (33%), and abdominal pain (14%). However, these rheumatological symptoms did not differ between the two groups. CONCLUSION: Although the rheumatological symptoms were not different between HCV patients with and without cryoglobulinaemia, HCV patients showed various rheumatological manifestations. These result suggests that HCV infection could be included as one of the causes in patients with unexplained rheumatological symptoms.  (+info)

A critical evaluation of enzyme immunoassays for detection of antinuclear autoantibodies of defined specificities. I. Precision, sensitivity, and specificity. (5/2145)

OBJECTIVE: To determine the performance characteristics of enzyme-based immunoassay (EIA) kits for the detection of antinuclear and other autoantibodies of defined specificities. METHODS: Nine manufacturers of EIA kits to detect antibodies of defined specificities participated in a study in which they received coded sera from the Centers for Disease Control and Prevention. These coded sera contained different dilutions of antibody of one specificity mixed with sera containing antibodies of other specificities. The manufacturers were asked to use their standard technology to determine antibody content and send the data to a committee of the International Union of Immunological Societies for analysis. The data were analyzed for sensitivity and specificity in the detection of anti-double-stranded DNA (anti-dsDNA), anti-single-stranded DNA, antihistone, anti-Sm, anti-U1 RNP, anti-SSA/Ro, anti-SSB/La, anti-Scl-70 (DNA topoisomerase I), anticentromere, and anti-Jo-1 antibodies. In addition, replicate samples were included in the coded sera to evaluate the precision of each EIA method. RESULTS: Lack of sensitivity and specificity was most evident in the anti-dsDNA and anti-Sm kits, although 2 kits for anti-dsDNA achieved acceptable sensitivity and specificity. Generally, anti-SSA/Ro, anti-SSB/La, anti-Scl-70, anticentromere, and anti-Jo-1 kits performed well. Many false-positive results were obtained with a multiple myeloma serum containing cryoprecipitates, but multiple myeloma sera without cryoprecipitates presented no problem in the EIA system. Precision, based on evaluation of replicate samples, varied from very good to poor. CONCLUSION: No single manufacturer was clearly superior to others in terms of their products' overall sensitivity, specificity, and precision. Areas that needed improvement were in kits for the detection of antibodies to dsDNA and to Sm antigen. Some EIA kits achieved good sensitivity and specificity. Individual manufacturers were informed of the performance of their respective kits so they could take measures to correct perceived deficiencies and thus improve the reliability of a group of important diagnostic assays used in the evaluation of systemic rheumatic diseases.  (+info)

Influence of ethnic background on clinical and serologic features in patients with systemic sclerosis and anti-DNA topoisomerase I antibody. (6/2145)

OBJECTIVE: To investigate the effect of ethnicity on clinical and serologic expression in patients with systemic sclerosis (SSc) and anti-DNA topoisomerase I (anti-topo I) antibody. METHODS: Clinical and serologic features, as well as HLA class II allele frequencies, were compared among 47 North American white, 15 North American black, 43 Japanese, and 12 Choctaw Native American SSc patients with anti-topo I antibody. RESULTS: The frequency of progressive pulmonary interstitial fibrosis was lower, and cumulative survival rates were better in white compared with black and Japanese patients. Sera of white and black patients frequently recognized the portion adjacent to the carboxyl terminus of topo I, sera of Japanese patients preferentially recognized the portion adjacent to the amino terminus of topo I, and sera of Choctaw patients recognized both portions of topo I. Anti-RNA polymerase II and anti-SSA/Ro antibodies were present together with anti-topo I antibody more frequently in sera of Japanese patients than in sera of white patients. The HLA-DRB1 alleles associated with anti-topo I antibody differed; i.e., DRB1*1101-*1104 in whites and blacks, DRB1*1502 in Japanese, and DRB1*1602 in Choctaws. Multivariate analysis showed that ethnic background was an independent determinant affecting development of severe lung disease as well as survival. CONCLUSION: Clinical and serologic features in SSc patients were strongly influenced by ethnic background. The variability of disease expression in the 4 ethnic groups suggests that multiple factors linked to ethnicity, including genetic and environmental factors, modulate clinical manifestations, disease course, and autoantibody status in SSc.  (+info)

Disparate T cell requirements of two subsets of lupus-specific autoantibodies in pristane-treated mice. (7/2145)

Intraperitoneal injection of pristane induces a lupus-like disease in BALB/c and other non-autoimmune mice characterized by autoantibody production and the development of immune complex disease closely resembling lupus nephritis. Two subsets of autoantibodies are induced by pristane: IgG anti-DNA DNA and -chromatin autoantibodies are strongly IL-6-dependent, whereas IgG anti-nRNP/Sm and -Su antibodies are not. The present studies were carried out to examine the role of T cells in establishing this dichotomy between the production of anti-nRNP/Sm/Su versus anti-DNA/chromatin autoantibodies. Autoantibody production and renal disease were evaluated in athymic (nude) mice treated with pristane. BALB/c nu/nu mice spontaneously developed IgM and IgG anti-single-stranded (ss)DNA and -chromatin, but not anti-nRNP/Sm or -Su, autoantibodies. Pristane treatment increased the levels of IgG anti-chromatin antibodies in nu/nu mice, but did not induce production of anti-nRNP/Sm or -Su antibodies. In contrast, BALB/c nu/+ and +/+ control mice did not spontaneously produce autoantibodies, whereas anti-nRNP/Sm and -Su autoantibodies were induced by pristane in approx. 50% of nu/+ and +/+ mice and anti-DNA/chromatin antibodies at lower frequencies. Nude mice spontaneously developed mild renal lesions that were marginally affected by pristane, but were generally milder than the lesions developing in pristane-treated nu/+ and +/+ mice. The data provide further evidence that two distinct pathways with different cytokine and T cell requirements are involved in autoantibody formation in pristane-induced lupus. This dichotomy may be relevant to understanding differences in the regulation of anti-DNA versus anti-nRNP/Sm autoantibodies in systemic lupus erythematosus, as well as the association of anti-DNA, but not anti-nRNP/Sm, with lupus nephritis.  (+info)

Electrocardiographic abnormalities in a murine model injected with IgG from mothers of children with congenital heart block. (8/2145)

BACKGROUND: It is a widely held view that congenital heart block (CHB) is caused by the transplacental transfer of maternal autoantibodies (anti-SSA/Ro and/or anti-SSB/La) into the fetal circulation. To test this hypothesis and to reproduce human CHB, an experimental mouse model (BALB/c) was developed by passive transfer of human autoantibodies into pregnant mice. METHODS AND RESULTS: Timed pregnant mice (n=54) were injected with a single intravenous bolus of purified IgG containing human anti-SSA/Ro and anti-SSB/La antibodies from mothers of children with CHB. To parallel the "window period" of susceptibility to CHB in humans, 3 groups of mice were used: 8, 11, and 16 days of gestation. Within each group, we tested 10, 25, 50, and 100 microg of IgG. At delivery, ECGs were recorded and analyzed for conduction abnormalities. Bradycardia and PR interval were significantly increased in 8-, 11-, and 16-day gestational groups when compared with controls (P<0.05). QRS duration was not significantly different between all groups. Antibody levels measured by ELISA in both mothers and their offspring confirmed the transplacental transfer of the human antibodies to the pups. CONCLUSIONS: The passive transfer model demonstrated bradycardia, first-degree but not complete atrioventricular block in pups. The greater percentage and degree of bradycardia and PR prolongation in the 11-day mouse group correlates with the "window period" of susceptibility observed in humans. The high incidence of bradycardia suggests possible sinoatrial node involvement. All together, these data provide relevant insights into the pathogenesis of CHB.  (+info)

Looking for Antinuclear Antibody Test? Find out information about Antinuclear Antibody Test. antinuclear antibody Explanation of Antinuclear Antibody Test
CLINICAL RESEARCH STUDY The Clinical Utility of a Positive Antinuclear Antibody Test Result Aryeh M. Abeles, MD, Micha Abeles, MD Division of Rheumatology, University of Connecticut Health Center, Farmington.
TY - JOUR. T1 - Application of the log-linear model in the prediction of the antinuclear antibody test in the dog.. AU - Kass, P. H.. AU - Strombeck, D. R.. AU - Farver, T. B.. AU - Ardans, A. A.. PY - 1985/11. Y1 - 1985/11. N2 - To find possible associations between antinuclear antibody (ANA) pattern, ANA titer, and certain clinical changes and clinical laboratory test results in dogs, the veterinary medical records of 111 ANA-positive and 126 ANA-negative dogs were examined. Variables could not be found that had significant associations with ANA pattern (unlike the results in persons), because of the predominance of 2 patterns. A log-linear model for ANA titer adequately fit the observed frequency and included 2-way interactions between titer and polyarthritis, titer and hematologic disorders, and polyarthritis and lymphadenopathy.. AB - To find possible associations between antinuclear antibody (ANA) pattern, ANA titer, and certain clinical changes and clinical laboratory test results in ...
The serological diagnosis of juvenile rheumatoid arthritis (JRA) is difficult, with only 7-10% of patients 19S IgM rheumatoid factor positive. About 60-70% of patients are positive for hidden 19S IgM rheumatoid factor, but this test requires serum separation and is not available in most laboratories. Antiperinuclear factor has been described in both seropositive and seronegative adult patients with rheumatoid arthritis, but has not been thoroughly evaluated in children with JRA. This study determined the diagnostic sensitivity and specificity of antiperinuclear factor in patients with JRA. Serum samples from 64 children with JRA, 24 with systemic lupus erythematosus (SLE), and 24 control subjects were tested for the presence of antiperinuclear factor. A total of 10 (83%) of seropositive, polyarticular onset and six (37%) of seronegative, polyarticular onset patients with JRA were positive for antiperinuclear factor. The occurrence of antiperinuclear factor in five (19%) with pauciarticular onset ...
Antinuclear Antibody Test Market by Product (Reagents & Assay Kits, Systems, Software, Services), Technique (Immunofluorescence, Elisa, Multiplex), Disease (Rheumatoid Arthritis, SLE), End User (Clinical Labs, Hospitals) - Forecast to 2021 is a market research report available at US $5650 for a Single User PDF License from RnR Market Research Reports Library.
The antinuclear antibody (ANA) test is widely used as a serological marker of autoimmune disease. Antinuclear antibodies are immunoglobulins or antibodies that bind to one or more antigens expressed within the nucleus of human cells. Used selectively, the ANA test can be a useful laboratory tool to help confirm or exclude the diagnosis of systemic rheumatic disease. However, the relatively high prevalence of ANAs in other inflammatory conditions, as well as healthy individuals, can make a positive result difficult to interpret.
Introduction The finding of antinuclear antibody (ANA) positivity in a wholesome individual is usually of unknown significance and generally is benign. Outcomes Overall, ANA amounts are considerably higher in females than in men which association retains in patients using the autoimmune illnesses lupus and arthritis rheumatoid (RA) aswell as in healthful controls (HC). Age group was not considerably connected with ANA amounts and the raised ANA values cannot be described by higher IgG amounts. Another autoantibody, anti- cyclic citrullinated peptide (CCP), didnt present gender dimorphism in arthritis rheumatoid (RA) or healthful people. The autoantigen array demonstrated significant elevations of various other autoantibodies in high ANA HCs. A few of these autoantibodies had been aimed to antigens in others and epidermis had been linked to autoimmune circumstances of kidney, joints or thyroid. Gene appearance analyses showed a larger prevalence of considerably upregulated genes in HCs with ...
TY - JOUR. T1 - Antinuclear antibodies. T2 - Clinical applications. AU - Wanchu, A.. PY - 2000/12/1. Y1 - 2000/12/1. N2 - One of the common serological hallmarks of autoimmune disorders is the presence of various autoantibodies in the sera of patients affected by these disorders. Antinuclear antibodies (ANA) detection is often needed to aid the diagnosis in several autoimmune disorders. In view of the different methodologies available for their detection, it becomes essential to understand the advantages and pitfalls of each procedure. This brief review discusses some methodological aspects of ANA detection and the clinical relevance of the presence of some of the autoantibodies found in the sera of patients with autoimmune disorders.. AB - One of the common serological hallmarks of autoimmune disorders is the presence of various autoantibodies in the sera of patients affected by these disorders. Antinuclear antibodies (ANA) detection is often needed to aid the diagnosis in several autoimmune ...
Fluorescent antinuclear antibody (ANA) testing was performed on 141 sera from 114 patients with well defined rheumatic diseases including fibrositis syndrome and 24 sera from 24 healthy subjects using HEp-2 cells and rat liver as substrates. ANA titers were almost always higher on HEp-2, in most cas …
The ANA test has a specificity of around 95 percent, meaning that of 20 people who test negative, 19 actually do not have lupus, but one has tested falsely negative and may have lupus. Its sensitivity is even less, meaning that more than one person out of 20 will test falsely positive but will not have lupus. False positive ANAs are fairly common and occur more frequently in older people, people with other autoimmune diseases, with viral infections, some chronic inflammations, and after taking some medications. Medications notorious for producing positive ANA tests are procainamide, used for some heart arrhythmias, and hydralazine, which used to be frequently prescribed to treat high blood pressure. Positive ANAs may persist for years after someone has taken these drugs.. The other common belief which I want to debunk here is that it is always important, even critical, to treat a disease early in the hopes of producing a cure. This belief probably stems from the emphasis we place on detecting ...
Hi I had a positive antinuclear antibody test that came back positive with 160 homogeneous when it is supposed to be at 40 I dont know my doctor has tested me for viral infections which came back negative what is clear to the doctor is that something is fighting me in my body attacking my joints Im going to a rhemuatologist but I dont know what to expect they will test me for lupus and so forth but the doctor doesnt think that is it and tested negative for rhuematoid arthiritis but I have some type of arthiris now from whatever has been attacking the funny thing is that I had some serious injuries bout three months I think knee and feet and fingers knuckles now since about a month ago they seem to be inflammatory not all at once though so far swelling is down on my knees but its not in my fingers and hand has anyone had these symptoms the only family history I have with diseases is leukemia which took my mums brother at an early age and Alzheimers on my dads side no arthiritic conditions ...
Objective: An Italian multicentre study was promoted in order to assess the accuracy of four anti-double-stranded DNA (dsDNA) antibody assays for SLE diagnosis and monitoring. Methods: Two hundred and twenty-three patients with established SLE according to ACR classification criteria were enrolled from 9 centres. They included 59 patients at first evaluation (disease duration ,12 months) and 164 with longer disease duration (median disease duration 120 months). The sera from 55 healthy subjects and 161 patients with rheumatic, infectious or neoplastic diseases were tested as controls. SLE activity was measured by ECLAM score. Anti-dsDNA antibodies were detected in serum by means of FarrzymeTM assay, fluoroenzymeimmunoassay (EliATM), Crithidia luciliae indirect immunofluorescence (CLIFT) or Farr radioimmunoassay (Farr). Cut-off values of quantitative assays were chosen by ROC curves analysis. Statistics were conducted by SPSS software package. Results: Sensitivity for SLE diagnosis ranged between ...
An ANA test detects antinuclear antibodies in your blood. Your immune system normally makes antibodies to help you fight infection. In contrast, antinuclear antibodies often attack your bodys own tissues - specifically targeting each cells nucleus.
To investigate the possible role of anti-ENA autoantibodies in the pathogenesis of SLE nephropathy, we performed a cross sectional clustering study of 91 SLE patients using 75 clinical and laboratory variables examining the presence of anti-dsDNA and ENA autontibodies by ELISA and Western blot. We applied principal component, hierarchical cluster, multiple correspondence and logistical regression analysis. Two polar forms of SLE nephropathy and five clinical groups were identified: group 1 without overt nephropathy (n = 37), group 2 with nephropathy and only proteinuria (n = 19), group 3 nephropathy and only hematuria (n = 11), group 4 with hematuria and proteinuria (n = 14) and group 5 on renal failure (n = 10). When analyzed individually, levels of anti-dsDNA and single anti-ENA antibodies did not allow us to differentiate between renal and non-renal groups. However, when the anti-ENA autoantibodies were analyzed as a cluster, a high predictive value for clinical nephropathy was obtained. ...
The results of antinuclear antibody tests using the indirect immunofluorescence technique may be reported as a description of the pattern and the intensity of fluorescence obtained at a certain dilution. If quantitative results are required titration is necessary. Such titrations may vary greatly between different laboratories. The present study involving 26 laboratories shows an improvement of interlaboratory comparability for the homogeneous fluorescence pattern when a common reference serum is used. Cultured cells as substrate appear to give better quantitative agreement than rat liver sections. National reference sera should be standardised in items of the appropriate WHO reference preparation. ...
Define antinuclear. antinuclear synonyms, antinuclear pronunciation, antinuclear translation, English dictionary definition of antinuclear. adj. 1. Opposing the production or use of nuclear power or nuclear weaponry; antinuke. 2. Reacting with the components of a cell nucleus: antinuclear...
Three IgM monoclonal anti-DNA antibodies were produced by hybridoma techniques from an MRL-lpr/lpr mouse using denatured DNA (dDNA) as the selection antigen. All three antibodies also bound poly(dT), poly(rA), and the single-stranded random copolymer poly(dI,dT), and each antibody displayed a unique preference for a limited array of other ribo- and deoxyribopolynucleotides based on direct binding as well as inhibition studies. Inability to identify a common primary structure in the polynucleotides reactive with each antibody suggested that higher ordered structures may be important. This notion was supported by the finding that oligomers of thymidine of 25-30 nucleotides or less were ineffective in blocking antibody binding to dDNA or poly(dT). However, deliberate destabilization of putative secondary structures by decreasing counterion concentration and increasing temperature had little effect on antibody binding to poly(dT). Since the antigenic polynucleotides in general contain little known ...
CDC Split Type: (blank) WAES0703USA03739. Write-up:Information has been received from a physician concerning his daughter, a female patient, with a penicillin allergy, who on 15-DEC-2006 was vaccinated with the first dose, 0.5ml, IM, of Gardasil. There was no concomitant medication. On approximately 15-JAN-2007 (/ 15-JAN-2007 ( one month later/) later) the physicians physicians daughter experienced /numbness numbness and pain in her right foot and leg./ leg. The physician reported that his daughter was examined by both a neurologist and a rheumatologist, and was not certain if she was /experie experiencing significant disability, but was waiting to see if the numbness and pain resolved. The physician reported there was some improvement. Additional information has been requested. 01-Jan-2007 magnetic resonance imaging, Negative, 01-Jan-2007 serum C-reactive protein test, Negative, 01-Jan-2007 serum antinuclear antibodies test, Positive ...
(2000) Ciccarelli et al. Multiple sclerosis (Houndmills, Basingstoke, England). We determined whether positive ANA was related to response to rIFNss-1a in 62 relapsing-remitting MS patients. According to the presence of antinuclear antibodies (ANA) at baseline and during the f... Case Studies in Systemic Sclerosis [4185368] - A 35- Year Old Woman with Puffy Hands, Raynauds Phenomenon, & Positive Antinuclear Antibody Test.- A 30- Year Old Woman with Puffy Hands, Raynauds Phenomenon, & Carpal Tunnel Syndrome.- A 22- Year Old Woman with Raynauds Phenomenon But No Other Symptoms & No Abnormalities on Examination.- A Young Adult With
The immune system makes an abundance of proteins called antibodies. Antibodies are made by white blood cells (B cells). The antibodies recognize and combat infectious organisms (germs) in the body. Antibodies develop in our immune system to help the body fight infectious organisms. When an antibody recognizes the foreign proteins of an infectious organism, it recruits other proteins and cells to fight off the infection. This cascade of attack is called inflammation.. Sometimes these antibodies make a mistake, identifying normal, naturally-occurring proteins in our bodies as being foreign and dangerous. When these antibodies make incorrect calls, identifying a naturally-occurring protein (or self protein) as foreign, they are called autoantibodies. Autoantibodies start the cascade of inflammation, causing the body to attack itself. The antibodies that target normal proteins within the nucleus of a cell are called antinuclear antibodies (ANA). Most of us have autoantibodies, but typically in ...
FIDIS (BMD, France) is a multiplex analytical flow cytometry system for the detection of antibodies. The aim of this study was to evaluate the FIDIS connective assay system for the detection of antinuclear antibodies (ANA), and to assess the clinical utility of these parameters in the diagnosis of connective tissue diseases. The FIDIS system simultaneously measures IgG antibodies directed at dsDNA, Ro, La, RNP, Sm, Jo-1, Scl-70, rRNP, and CENP-B. ...
A positive antinuclear antibodies, or ANA, test result can change to negative, especially in people who have short-term viral infections, according to the American College of Rheumatology. However,...
An ENA (Extractable Nuclear Antigen Antibodies) panel detects the presence of one or more specific autoantibodies in the blood. Autoantibodies are produced when a persons immune system mistakenly targets and attacks the bodys own tissues. This attack can cause inflammation, tissue damage, and other signs and symptoms that are associated with an autoimmune disorder.. ENA are a subset of antinuclear antibodies (ANA), antibodies directed against proteins found in the nucleus of cells. Certain autoimmune disorders are characteristically associated with the presence of one or more extractable nuclear antigen antibodies. This association can be used to help diagnose an autoimmune disorder and to distinguish between disorders.. The ENA panel is typically a group of 6-10 autoantibody tests. The number of tests offered will depend on the laboratory and the needs of the doctors and patients it serves. ENA panel tests, and other less common ENA tests, may be able to be ordered separately depending on the ...
BACKGROUND: Morphea is an inflammatory autoimmune skin sclerosis of unknownetiology. A causative role of Borrelia burgdorferi infection has beencontroversially discussed, but no conclusive solution has yet been achieved. OBJECTIVE: Intrigued by 3 young patients with severe Borrelia-associated morpheaand high-titer antinuclear antibodies, we retrospectively examined therelationship between Borrelia exposure, serologic autoimmune phenomena and ageat disease onset in morphea patients. METHODS: In 90 morphea patients thepresence of Borrelia-specific serum antibodies was correlated to the age atdisease onset and the presence and titers of antinuclear antibodies. Patientswith active Borrelia infection or high-titer antinuclear antibodies due tosystemic sclerosis or lupus erythematosus served as controls. RESULTS: We observed a statistically highly significant association between morphea, serologic evidence of Borrelia infection, and high-titer antinuclear antibodies when disease onset was in childhood ...
Teague, P O. and Friou, G J., Antinuclear antibodies in mice. II. Transmission with spleen cells, inhibition or prevention with thymus or spleen cells. (1969). Subject Strain Bibliography 1969. 1389 ...
A short alternative open reading frame named ORF7a has recently been discovered within the nucleocapsid gene of the porcine reproductive and respiratory syndrome virus (PRRSV) genome. Proteins (7ap) translated from the ORF7a of two divergent strains - a type I and a type II - are able to completely reduce the motility of nucleic acids at relatively high molar charge ratios in gel retardation assays indicating strong dsDNA- and ssRNA-binding capability. Conserved RNA- and DNA-binding properties suggest that nucleic acid binding is a functional property of the divergent 7aps, and not an arbitrary consequence of their net positive charge. Sera from Hu7ap-immunised pigs and mice did not react with Hu7ap or Hu7ap-GFP; however, antinuclear antibodies were detected in the sera of the immunised animals, suggesting an ability of Hu7ap to interact with or mimic autoantigenic macromolecules. ...
When purified under rigorous conditions, some murine anti-double-stranded-DNA (anti-dsDNA) antibodies actually bind chromatin rather than dsDNA. This suggests that they may actually be antinucleosome antibodies that only appear to bind dsDNA when they are incompletely dissociated from nucleosomes. Experiments in murine models suggest that antibody-nucleosome complexes may play a crucial role in the pathogenesis of glomerulonephritis in systemic lupus erythematosus. Some human monoclonal anti-DNA antibodies are pathogenic when administered to mice with severe combined immunodeficiency (SCID). Our objective was to achieve stable expression of sequence-altered variants of one such antibody, B3, in Chinese hamster ovary (CHO) cells. Purified antibodies secreted by these cells were tested to investigate whether B3 is actually an antinucleosome antibody. The pathogenic effects of the antibodies were tested by implanting CHO cells secreting them into SCID mice. Purified B3 does not bind to dsDNA unless
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TY - JOUR. T1 - Ligand recognition by anti-DNA autoantibodies. Affinity, specificity, and mode of binding. AU - Swanson, Patrick C.. AU - Ackroyd, Christine. AU - Glick, Gary D.. PY - 1996/2/6. Y1 - 1996/2/6. N2 - Understanding the molecular basis of DNA recognition by anti-DNA autoantibodies is a key element in defining the role of antibody·DNA complexes in the pathogenesis of the autoimmune disorder systemic lupus erythematosus. As part of our efforts to relate anti-DNA affinity and specificity to antibody structure, and ultimately to disease pathogenesis, we have generated a panel of eight anti-DNA mAbs from an autoimmune MRL MpJ- lpr/lpr mouse and have assessed the binding properties of these antibodies. We find that none of our anti-DNA mAbs bind to RNA and only one low-affinity mAb cross-reacts with non-DNA antigens, albeit weakly. None of the mAbs in our panel bind double-stranded DNA exclusively. Antibodies that recognize single-stranded DNA can be categorized into two groups based on ...
The ANA test is ordered when someone shows signs and symptoms that are associated with a systemic autoimmune disorder. People with autoimmune disorders can have a variety of symptoms that are vague and non-specific and that change over time, progressively worsen.The antinuclear antibody (ANA) test is used as a primary test to help evaluate a person for autoimmune disorders that affect many tissues and organs throughout the body (systemic) and is most often used as one of the tests to help diagnose systemic lupus erythematosus (SLE). ANA are a group of autoantibodies produced by a persons immune system when it fails to adequately distinguish between self and nonself. They target substances found in the nucleus of a cell and cause organ and tissue damage. Depending on a persons signs and symptoms and the suspected disorder, ANA testing may be used along with or followed by other autoantibody tests. ...
ResearchMoz presents professional and in-depth study of Autoimmune Disease Diagnostics Market: (By Test Types: Antinuclear Antibody Test, Autoantibody Test, Complete Blood Count, Comprehensive Metabolic Panel, C-Reactive Protein Test, Erythrocyte Sedimentation Rate, Urinalysis and Others; Disease: Graves Disease, Hashimotos Thyroiditis, Rheumatoid Arthritis, Multiple Sclerosis, Systemic Lupus Erythematosus, Type 1 Diabetes, and Others): Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2015 - 2023.. This report on the autoimmune disease diagnostics market studies the current and future prospects of the global market. Autoimmune disease diagnostics market includes various laboratory tests that are performed to diagnose autoimmune disorders. These tests include blood tests for one or more autoantibodies and tests for inflammation. The rising prevalence of autoimmune diseases, increase in health care expenditure globally, rising government initiatives and increasing automation ...
Any antibody to nuclear components is an ANA. Most patients with ANAs do not have SLE, but most people with SLE have ANAs. The most common screening test is IIF on rodent liver or human epithelial (HEp2) tissue,3 although ELISA tests are available.4,5 Lupus erythematous cells simply represent nuclei opsonised by ANAs and are no longer used in diagnosis. Although ANAs are very sensitive for SLE, positive ANAs are common, especially in unwell elderly individuals.6-8 Therefore, ANAs have low PPV for SLE in unselected populations or when present in low titres,6,9 and are not diagnostic. One in three healthy people have detectable ANAs on HEp-2 cells at a screening dilution of 1/40 and one in 20 will be positive at 1/160. HEp-2 cells produce more positive ANAs than rat tissue, and some ANAs (for example, anticentromere antibodies) can only be reliably detected on HEp-2 substrate. Although ANA negative SLE is reported,10 it is not clear whether this is the result of a technical artifact or whether a ...
Hello, Few of my joints hurt occasionally. My blood test returned: positive ANA, speckled pattern (all other arthritis tests are normal). Two years ago, I had negative ANA, so I assume Im not i...
Question - Chronic pain in abdominal area, tiredness, joint pain, bloating. Tests showed positive ANA, hypothyroid, elevated inflammation. Suggestion?. Ask a Doctor about diagnosis, treatment and medication for Abdominal pain, Ask an Orthopaedic Surgeon
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my 12 year old daughter has been having pain in her back,hips,knees and feet. she had a ana titer speckled pattern result of 1:160. retest? Answered by Dr. Joseph Woods: Likely no need,,,: This is apparently a very positive result that, cou...
HSV1 + HSV2 Nuclear antibody [0119] for ICC/IF. Anti-HSV1 + HSV2 Nuclear mAb (GTX36659) is tested in Herpes simplex virus samples. 100% Ab-Assurance.
years with a mean 38.4 years. The duration of the disease ranged from 1-30 years with the mean of 12.3 years. All patients were subjected to full history taking, complete clinical examination, radiological investigation and laboratory investigation with stress on detection of ANA by indirect immuno-flourescence test. Ten normal volunteers were taken as a control group to evaluate the accuracy of the analysed material. ANA test was found [+ve] in 27.6% of rheumatoid patients, two ANA patients, homogeneous and speckled were detected. It was found also that the speckled pattern had a higher percentage, 87.5% while the homogeneous type had a lower percentage 13.5%. The incidence of ANA presence increases in females and also increases with the presence of erosive changes, subcutaneous nodules and Sjogrens syndrome ...
If ANA is POSITIVE, a reflex ANA titer is always performed. ANA Reflex to additional ENA/dsDNA testing is not an orderable test at Seattle Childrens Hospital. Additional specific tests can be ordered at time of ANA collection, or, alternatively, all ANA serum is saved for 6 months. If dsDNA/ENA testing is desired after initial ANA testing, order as an Add-on Communication in CIS or call laboratory (206) 987-2102.. ...
Mouse monoclonal ds DNA antibody [35I9 DNA] validated for ELISA, IHC, ICC, Dot, ICC/IF. Referenced in 18 publications and 3 independent reviews. Immunogen…
This test guide provides information about tests that may be useful for the diagnosis of autoimmune diseases. Three screening approaches that use antinuclear antibody and specific antibody tests are discussed.
The immunofluorescent antinuclear antibody (ANA or FANA) test is positive in almost all individuals with systemic lupus (97 percent), and is the most sensitive diagnostic test currently available for confirming the diagnosis of systemic lupus when accompanied by typical clinical findings. When three or more typical clinical features are present, such as skin, joint, kidney, pleural, pericardial, hematological, or central nervous system findings as described above, a positive ANA test confirms the diagnosis of systemic lupus ...
Analysis of somatic mutations revealed that induction of anti-dsDNA autoantibodies from SLE patients are antigen driven and thus T cell dependent. Since DNA per se has repeatedly been shown not to be immunogenic, various mechanisms leading to the production of anti-dsDNA-antibodies have been discuss …
It is our premise that JDMS is a distinct disease entity and that the increase in HLA-B8 and -DR3 in JDMS places this disease in the company of other immunopathic disorders. There are conflicting data concerning immunological abnormalities in JDMS , but there appears to be impairment of natural killing and evidence of complement activation. The frequent positive ANA in JDMS raises the speculation of its relationship to the antinuclear antibody, Jo-1, found in some adults with PM, which has specificity for tRNAHis. Most newly diagnosed JDMS patients have antibodies to Coxsackie B which may be related to the pathogenesis of this disease. Specific pathological findings of endothelial cells containing reticulotubular inclusions are associated with vessel occlusion, subsequent obliteration and increased Factor VIII levels in clinically active disease ...
The medical institutes who establish diagnostic standards say that, you probably have Lupus if you have two or more of these symptoms plus a positive ANA (antinuclear antibodies) blood test, OR if you have four or more of these symptoms with no other reason for them ...
ANA also goes up and down all the time. The info and advice in the above posts is excellent. And the funny thing is that I can feel like crap and my ANA will be relatively low, then feel great and its high. So go figure. I hope thatyour other test are normal and that its just the way you are and not any illness. My ANA was elevated for over a decade and probably longer with no symptoms, then I developed diabetes with NASH and endocrine problems and all of that was still unrelated to my ANA according to my docs. then I developed sjogrens syndrome, a relatively mild form of autoimmune disease. I believe the interferon I took caused my autoimmune disease to become active. I think otherwise I would have continued to havean elevated ANA with no symptoms for the rest ofmy life otherwards ...
SUMMARY. Twenty patients with unexplained pulmonary fibrosis and circulating antinuclear factor were identified. Diseases commonly associated with antinuclear factor, such as systemic lupus erythematosus or scleroderma, were excluded. Half of the patients showed typical restrictive ventilatory defects and X-ray evidence of severe fibrotic changes. The remaining patients predominantly had evidence of obstructive airway diseases.. Four lung biopsies were available. Three showed histological evidence of interstitial pulmonary fibrosis. Immunofluorescent study of one of the biopsy sections with pulmonary fibrosis from a patient with only IgM serum antinuclear factor showed IgM in the alveolar septa. Relatively little fibrosis was present in immunoglobulin-laden areas whereas no immunoglobulin could be demonstrated in the adjacent alveolar septa showing marked fibrosis. Seven out of 10 patients with X-ray evidence of severe interstitial pulmonary fibrosis and typical restrictive ventilatory defects ...
Objective: To investigate the rote of anti-nucleosome antibodies (AnuA) in systemic lupus erythematosus (SLE).. Methods: IgG anti-nucleosome antibodies were detected by enzyme-linked immunosorbent assay (ELISA) in the sera of 233 SLE and 220 other rheumatic diseases′ patients as well as 31 health controls. The patients were also evaluated for clinical and biological parameters.. Results: (1) Out of 233 SLE patients, 144 (61.8%) were seropositive for AnuA, which was significantly higher than that of patients with other rheumatic diseases [2.7% (6/220), P < 0.001]; the sensitivities and specificities of AnuA in SLE were 61.8% and 97.6%, respectively. (2) The positive rated of AnuA in SLE lacking of anti-DNP, anti-cmDNA, anti-Sm and anti-dsDNA antibodies were 57.1%, 55.9%, 62.4% and 51.2%, respectively. (3) The frequency of the fever, skin rash, and arthralgia were significantly higher in 144 positive AnuA SLE than those in AnuA negative SLE (P < 0.05). The frequency of leukopenia, elevations of ...
Clinical trial for SYSTEMIC LUPUS ERYTHEMATOSUS , Study of the Safety and Efficacy of GDC-0853 in Participants With Moderate to Severe Active Systemic Lupus Erythematosus
NLRP3 inflammasome has been implicated in the pathogenesis of systemic lupus erythematosus (SLE). The activation of NLRP3 inflammasome results in the production of IL-1β and the subsequent inflammation. Anti-dsDNA antibodies (anti-dsDNA Abs) play critical roles in the development and progression of SLE. However, the mechanism of NLRP3 inflammasome activation in SLE is still not known. This study investigated the activation of NLRP3 inflammasome stimulated by anti-dsDNA Abs in monocytes/macrophages from SLE patients. Monocytes/macrophages from SLE patients or healthy controls were stimulated with anti-dsDNA Ab-positive serum or purified anti-dsDNA Abs. Activation of inflammasome was measured by flow cytometry or Western blot. Anti-dsDNA Abs isolated from active SLE patients were injected into female (NZB × NZW) F1 mice and the activation of NLRP3 inflammasome and the frequencies of Th17 and Treg were examined. The activity of caspase-1 was significantly increased in active SLE patients and was
IgMa anti-single-stranded DNA (anti-ssDNA), antihistone, antichromatin, and anti-Sm autoantibodies (autoAb) in VH3H9R/VLκ8R.B6, VH3H9R/VLκ8R.B6.129chr1b129/
Antinuclear antibodies are associated with rheumatic diseases including Systemic Lupus Erythematous (SLE), mixed connective tissue disease, Sjogrens syndrome, scleroderma, polymyositis, CREST syndrome, and neurologic SLE. ...
Skin testing can confirm many common types of allergies. Among the most helpful are the anti-Sm and anti-ds-DNA, because when positive, they strongly suggest SLE. I was told I had Lupus because of a positive ANA. Symptoms of ANA-positive rheumatic diseases are listed in TABLE 1. Read on to learn more about each type. 0 comment . Intradermal Skin Test . Typically, if a person tests positive for the antinuclear antibody, it means only that the person could have lupus. Your immune system normally makes antibodies to help you fight infection. In intradermal (under the skin) testing, the doctor or nurse injects a tiny amount of allergen into the outer layer of skin. My doctor and I both felt like we were finally getting to the bottom of things and he referred me to a rheumatologist, but by the time I could get in to see him, I was well again (6 weeks). last one 1:320 speckled) with an elevated CRP xs 3 (1.2 - 1.8) a slightly elevated SED rate of 37. I never had allergy symptoms and now I react to so ...
Abstract OBJECTIVE/HYPOTHESIS: The aim of this prospective study is to evaluate the possible association between Ménières disease (MD) and autoantibodies. METHODS: Fifty-five patients with definite MD (51 unilateral and 4 bilateral) were matched with 55 patients with unilateral vestibular paresis without cochlear involvement and 55 healthy subjects. Blood samples were collected from all study subjects for the determination of serum TSH, free triiodothyronine, free thyroxine, anti-TSH receptor antibody, antithyroperoxidase antibody, antithyroglobulin antibody and of antibodies to non-organ-specific antigens, namely antinuclear antibodies, antibodies to extractable nuclear antigens and antineutrophilic cytoplasmic antibodies. RESULTS: Thirty-three subjects (60%) of the MD group had 1 or more elevated serum autoantibody levels, both organ and non-organ specific; 16 patients (29.1%) with unilateral vestibular paresis had 1 or more elevated serum autoantibody levels, while 13 healthy subjects ...
Objective: Rheumatic diseases with involvement of the central nervous system (RDwCNS) may mimic multiple sclerosis (MS). Inversely, up to 60% of MS-patients have antinuclear autoantibodies (ANAs) and may be misdiagnosed as RDwCNS. The detection of antibodies against extractable nuclear antigens (ENA) and oligoclonal bands (OCB) are established valuable diagnostic tools in the differential diagnosis of RDwCNS and MS. The MRZ-reaction (MRZR) is defined by three antibody indices (AIs) against neurotropic viruses and is frequently positive in MS. To investigate the added value of MRZR combined with testing for antibodies against ENAs and OCB detection to distinguish RDwCNS from ANA positive MS ...
TY - JOUR. T1 - Congenital heart block not associated with anti-Ro/La antibodies. T2 - Comparison with anti-Ro/La-positive cases. AU - Brucato, Antonio. AU - Grava, Chiara. AU - Bortolati, Maria. AU - Ikeda, Keigo. AU - Milanesi, Ornella. AU - Cimaz, Rolando. AU - Ramoni, Veronique. AU - Vignati, Gabriele. AU - Martinelli, Stefano. AU - Sadou, Youcef. AU - Borghi, Adele. AU - Tincani, Angela. AU - Chan, E. K L. AU - Ruffatti, Amelia. PY - 2009/8. Y1 - 2009/8. N2 - Objective. To study anti-Ro/La-negative congenital heart block (CHB). Methods. Forty-five fetuses with CHB were evaluated by analysis of anti-Ro/La antibodies using sensitive laboratory methods. Results. There were 9 cases of anti-Ro/La-negative CHB; 3 died (33.3%). Only 3 (33.3%) were complete in utero and 5 (55.5%) were unstable. No specific etiology was diagnosed. Six infants (66.6%) were given pacemakers. There were 36 cases of anti-Ro/La-positive CHB. All except 2 infants (94.4%) had complete atrioventricular block in utero. Ten ...
Nearly 75% of patients receiving procainamide therapy will develop a positive ANA test within the first year of treatment, and over 90% develop a positive ANA
A progressively wide range of studies exist into the association of Anti-Nuclear Antibodies (ANAs) with Autoimmune Rheumatic Diseases (ARDS), such as Systemic Lupus Erythematosus (SLE), Sjogrens Syndrome (SjS), Systemic Scleroderma (SS), Rheumatoid Arthritis (RA), Reynauds phenomena (RP) to name but a few of the more serious and debilitating ARDs1-4. It is thought that a single base mutation in the gene responsible for anti-Phosphotidylcholine (aPTC), (an IgM Natural Autoantibody (NAb) responsible for clearance of cellular debris) is a likely mechanism by which ANA pathogenesis may originate5. Visualization of these antibodies through Indirect Immuno-Fluorescence (IIF) has long remained the principle detection path, the Gold Standard. The objective of this study is to investigate the replacement of IIF with ELISA as a screening/diagnostic method in the ongoing effort to eliminate the time-consuming and subjective nature of IIF; ELISA being easily adapted to automation. 350 routine anonymised ...
TY - JOUR. T1 - Hydronephrosis associated with antiurothelial and antinuclear autoantibodies in BALB/c-Fcgr2b-/-Pdcd1-/- mice. AU - Okazaki, Taku. AU - Otaka, Yumi. AU - Wang, Jian. AU - Hiai, Hiroshi. AU - Takai, Toshiyuki. AU - Ravetch, Jeffrey V.. AU - Honjo, Tasuku. PY - 2005/12/19. Y1 - 2005/12/19. N2 - Because most autoimmune diseases are polygenic, analysis of the synergistic involvement of various immune regulators is essential for a complete understanding of the molecular pathology of these diseases. We report the regulation of autoimmune diseases by epistatic effects of two immunoinhibitory receptors, low affinity type IIb Fc receptor for IgG (FcγRIIB) and programmed cell death 1 (PD-1). Approximately one third of the BALB/c-Fcgr2b-/-Pdcd1-/- mice developed autoimmune hydronephrosis, which is not observed in either BALB/c-Fcgr2b-/- or BALB/c-Pdcd1-/- mice. Hydronephrotic mice produced autoantibodies (autoAbs) against urothelial antigens, including uroplakin IIIa, and these antibodies ...
Prolactin is a peptide hormone produced by the anterior pituitary gland that is critical in lactation. Prolactin can also be produced by lymphocytes, and both B and T cells express prolactin receptors. These findings have suggested that prolactin has immunomodulatory functions. Studies in spontaneously autoimmune hosts have demonstrated a role for prolactin in augmenting autoreactivity. We chose to analyze prolactin effects on anti-DNA B cells in nonspontaneously autoimmune female BALB/c mice transgenic for the heavy chain of an anti-DNA antibody. Treatment with prolactin for 4 weeks induced a lupus-like phenotype with an increased number of transgene-expressing B cells, elevated serum anti-DNA antibody titers, and glomerular immunoglobulin deposits. Prolactin caused a decrease in the population of transitional B cells and an increase in mature follicular and marginal zone B cells. The DNA-reactive B cells had a follicular cell phenotype. Anti-DNA hybridomas demonstrated that prolactin alters ...
Pathology and Laboratory Medicine is a chapter in the book, Rheumatology, containing the following 24 pages: Bone Turnover Biochemical Marker, Antinuclear Antibody, ANA Staining Pattern, Anticentromere Antibody, Anti-Double Stranded DNA Antibody, Anti-Single Stranded DNA Antibody, Antihistone Antibody, Anti-Jo-1 Antibody, Anti-Ku Antibody, Anti-Mi-2 Antibody, Anti-ribosomal P Antibody, Anti-ribonucleoprotein Antibody, Anti-Topoisomerase I Antibody, Anti-Smith Antibody, Anti-Ro Antibody, Anti-La Antibody, Antineutrophil Cytoplasmic Antibody, Antiphospholipid Antibody, Lupus Anticoagulant, Synovial Fluid, Polarized Microscopy, Synovial Fluid White Blood Cell Count, Rheumatoid Factor, Major Histocompatibility Complex Antigens.
Objective. To analyze the clinical value of anti-DFS70 antibodies in a cohort of patients undergoing routine antinuclear antibodies (ANAs) testing. Methods. Sera with a dense fine speckled (DFS) indirect immunofluorescence (IIF) pattern from 100 consecutive patients and 100 patients with other IIF patterns were tested for anti-DFS70 antibodies by a novel chemiluminescence immunoassay (CIA) and for ANA by ANA Screen ELISA (both INOVA). Results. Among the 100 patients with a DFS IIF pattern, 91% were anti-DFS70 positive by CIA compared to 3% in the comparator group . The CIA and IIF titers of anti-DFS antibodies were highly correlated (rho = 0.89). ANA by ELISA was positive in 35% of patients with the DFS IIF pattern as compared to 67% of patients with other patterns . Only 12.0% of patients with DFS pattern and 13.4% with DFS pattern and anti-DFS70 antibodies detected by CIA had systemic autoimmune rheumatic disease (SARD). Only 5/91 (5.5%) patients with anti-DFS70 antibodies had SARD and their sera
LU, Zhimin et al. Altered peripheral lymphocyte subsets in untreated systemic lupus erythematosus patients with infections. Braz J Med Biol Res [online]. 2019, vol.52, n.4, e8131. Epub 15-Abr-2019. ISSN 1414-431X. The leading cause of death in systemic lupus erythematosus (SLE) patients is infection. The objective of this study was to evaluate the distribution of lymphocyte subsets in untreated SLE patients with infections. This was a cross-sectional study. Data from January 2017 to May 2018 were collected. Flow cytometry was used to measure the peripheral lymphocyte subsets including CD3+T cells, CD4+T cells, CD8+T cells, CD19+B cells, CD3-CD16+CD56NK cells, and CD3+CD16+CD56NKT cells in 25 healthy controls and 52 treatment-naive SLE patients, among whom 13 were complicated with infections. Association between the lymphocyte subsets and infections was further analyzed. SLE patients with infections (n=13) showed a significantly higher incidence rate of ...
Group A only: patients on immunosuppressive treatments had them withdrawn at baseline. All patients were allowed up to 160 mg depomedrol at baseline which could be repeated within two weeks up to a total of 4 shots maximum or until satisfactory improvement. Time to flare was calculated from baseline. moderate disease at baseline was defined as up to 3 BILAG B (moderate disease) organ scores, no BILAG A (severe disease) score and a SLEDAI ,/= 10. Severe disease required ,3 BILAG B, OR at least one BILAG A OR SLEDAI , 10 or meeting criteria for a severe flare on the SELENA SLEDAI flare index. At baseline 25 patients with moderate disease. 16 patients had severe disease. Note: severe rash with A on BILAG is only SLEDAI=2, explaining some discrepancies in measures ...
The antinuclear antibodies (ANA) test has been a cornerstone of the evaluation of connective tissue disease. The aim of this study was to investigate the diagnostic value of the ANA test in pleural or pericardial effusions of unknown causes. Over a 3-yr period, a total of 126 pleural fluid and 30 pericardial fluid samples were analysed. ANA tests were performed using a commercially available kit. The ANA kit used an indirect immunofluorescent antibody method with a human epithelial (HEP-2) cell line as substrate. Patients with high fluid ANA titre (,1:160) received a second aspiration 2 weeks after the initial aspiration if diagnosis was not confirmed. ANA results were positive in 39 pleural and 10 pericardial fluid samples. All but one of the effusions with positive ANA testing were exudative. Eleven pleural or pericardial effusions due to active systematic lupus erythematosus were identified and all had high ANA titres (1:160) with various staining patterns. Thirty-eight of 145 patients (26%) ...
TY - JOUR. T1 - Delineation of the human systemic lupus erythematosus anti-Smith antibody response using phage-display combinatorial libraries. AU - Del Rincon, I.. AU - Zeidel, M.. AU - Rey, E.. AU - Harley, J. B.. AU - James, J. A.. AU - Fischbach, M.. AU - Sanz, I.. PY - 2000/12/15. Y1 - 2000/12/15. N2 - The anti-Smith (Sm) autoantibody response is highly specific for systemic lupus erythematosus and is predominantly targeted to the Sm-B/B and -D1 polypeptides. In all animal species thus far studied, anti-Sm Abs initially recognize proline-rich epitopes in the carboxyl terminus of the Sm-B/B protein and subsequently to multiple other epitopes in B/B and D. The absence of appropriate mAbs has limited our understanding of the genetic and structural basis of this autoimmune response. Using phage-display technology and lymphocytes from a systemic lupus erythematosus patient we have generated the first and only panel of human IgG anti-Sm mAbs thus far available. These Abs reproduced to a ...
TY - JOUR. T1 - Anti-nuclear antibody reactivity in lupus may be partly hard-wired into the primary B-cell repertoire. AU - Chang, Sooghee. AU - Yang, Liu. AU - Moon, Young Mee. AU - Cho, Young Gyu. AU - Min, So Youn. AU - Kim, Tae Joo. AU - Kim, Young Joo. AU - Patrick, Wilson. AU - Kim, Ho Youn. AU - Mohan, Chandra. PY - 2009/10. Y1 - 2009/10. N2 - When monoclonal ANAs and non-ANAs generated from a genetically simplified mouse model of lupus, B6.Sle1, were recently compared, the ANAs exhibited three sequence motifs in their immunoglobulin heavy chains, including increased cationicity in CDR3 (motif A), reduced anionicity in CDR2 (motif B) and increased aspartate at H50 (motif C). The present study was designed to elucidate the extent to which these ANA-associated sequence motifs might be hard-wired into the primary B-cell repertoire in lupus. The immunoglobulin heavy chain sequence of total splenic B-cells, follicular B-cells and marginal zone B-cells from B6.Sle1 congenic mice and ...
OBJECTIVE: To compare the clinical, laboratory, and demographic variables of women in our clinic with systemic lupus erythematosus (SLE) who have had a pregnancy resulting in a live birth and identify any correlations with either term or preterm delivery. METHODS: Pregnancies in women with SLE from 1999 to 2001 were retrospectively reviewed. We recorded demographic data, disease activity (SLE Disease Activity Index, SLEDAI), obstetric history, prednisone dosage, other medications taken during pregnancy, history of renal disease, and autoantibody status [including antinuclear antibody, anti-DNA, anticardiolipin IgG (aCL), and lupus anticoagulant (LAC)]. Preterm delivery was defined as gestational age at delivery , 37 weeks. We performed a literature survey using PubMed and the key words SLE, pregnancy, and outcome. RESULTS: Of the 72 pregnancies, 28 (38.9%) resulted in preterm deliveries. There were no significant differences in any demographic or disease variables measured comparing term versus ...
Congenital heart block (CHB) is associated with maternal antibodies to Ro (SSA) and La (SSB) that cross the placenta and damage the AV node of fetus. An asymptomatic 27-year-old primigravida, diagnosed with second degree fetal heart block was investigated and found positive for antinuclear antibodies (ANA), anti-Ro and anti-La antibodies. Fetal echocardiography (2D-echo) showed no structural defect but bradycardia. The patient was started on dexamethasone 8mg/day. She had preterm premature rupture of membranes (PPROM) at 34 weeks and underwent emergency cesarean section (LSCS). The baby was born with complete heart block but was managed conservatively in view of low birth weight. The infant is now 5 months old and permanent pacemaker implantation is planned. ...
Patients with drug-related lupus erythematosus produce antibodies to nuclear histones which can be detected by a three-step indirect immunofluorescence technique. Procainamide-related antinuclear antibodies were detected by this technique, but hydralazine-related antinuclear antibodies were not. Certain evidence suggests that antibodies induced by the two drugs are reactive with different subclasses of histones. Hydralazine was shown to interact with a soluble DNA-histone complex, and the resulting interaction rendered the histone moiety resistant to trypsin digestion. This mechanism may help to maintain DNA-histone complexes in a potentially immunogenic form and result in the production of autoantibodies ...
A:Paxil may cause various negative effects in depression patients. I took lisinopril for 2 days and ended up with a huge top lip? Jai vu mon MD et lui ma dit quil ny à rien à faire? Randomized controlled study of TIPS versus paracentesis plus albumin in cirrhosis with severe ascites? The first one is the name as Pfizer still has the property of the name. Conditions associated with hypoglycemia include debilitated physical condition, buy flonase drug interactions, malnutrition, uncontrolled adrenal insufficiency, pituitary insufficiency or hypothyroidism! The Gale Encyclopedia of Medicine: Antinuclear antibody test! Pour pouvoir bénéficier de certaines fonctionnalités des Sites et Services, lutilisateur fournit, par le biais dun formulaire (avec un bouton de validation ou une case à cocher mentionnant son accord explicite), des données à caractère personnel potentiellement identifiantes telles que, par exemple, ses coordonnées ou son adresse de messagerie électronique (e-ma? After ...
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Discussion. The focus of care is to reduce the inflammatory effect of the cervical capsules or facetal region, which may traction the adjacent spinal nerve roots and centralize the diffuse pain picture. Emphasis on patient education is required. The postural changes we see may be of a cumulative nature and contribute to mid-back pain as a tendonosis strain and ligamentous sprain, also due to her tiny size in repeated lifting attempts. Synovitis of a rheumatoid arthritic nature, or member of an inflammatory arthopathy family, is strongly suspected. Additionally, if the antinuclear antibody titer is high, systemic lupus is suspected. If she develops dry mouth, dry eye, dry cough or sicca syndrome, present with a rheumatism, SLE or connective-tissue disease, we may have an overlap in conditions. Similarly, myositis is frequently a part of inflammatory arthopathies. Thus, my clinical differential diagnosis would be a form of rheumatoid arthrosis. A patient such as this should acquire baseline ...
OBJECTIVE: To determine the frequency of prolonged remission in systemic lupus erythematosus (SLE) using strict criteria for remission and to define disease characteristics and prognosis of patients achieving this state. To also determine the frequency of remission utilizing less restrictive definitions, such as allowing shorter period of disease quiescence, persistence of serological activity, or treatment in the absence of clinical disease. METHODS: Patients registered in the Lupus Clinic database between 1970 and 1997 with visits no more than 18 months apart were identified. Prolonged remission was defined as a 5-year consecutive period of no disease activity (SLE disease activity index, SLEDAI = 0) and without treatment (corticosteroids, antimalarials, or immunosuppressants). Prolonged serologically active, clinically quiescent (SACQ) was defined as active serology (elevated anti-dsDNA by Farr assay or hypocomplementemia) but no clinical activity on SLEDAI and no treatment. RESULTS: Seven ...
Systemic lupus erythematosus (SLE) is a heterogeneous, inflammatory, multisystem autoimmune disease in which antinuclear antibodies occur (often years...
Objective(s): Apoptosis is a tightly regulated process and plays a crucial role in autoimmune diseases. Because abnormalities in apoptosis are considered to be involved in the pathogenesis of systemic lupus erythematosus (SLE), in present study we studied the apoptosis in T lymphocytes from Iranian SLE patientsat protein and gene expression levels for some molecules which are involved in apoptosis pathways. Materials and Methods: Thirty five SLE patients (23 female, 12 male), and 20 age matched controls (10 female, 10 male) participated in this study. T lymphocytes were isolated from peripheral blood mononuclear cells (PBMCs) using MACS method. Apoptosis rate was studied at protein level by flow cytometer using Annexin V, and at gene expression level using semi-quantitative RT-PCR method for detection of Fas, FasL, Bcl-2, caspase 8, and caspase 9 genes. Results: The percentage of apoptotic cells in SLE patients was not different in comparison with controls (20.2% ± 1.4 vs 21.1% ± 1.0), but the
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Extractable Nuclear Antigen Antibodies (RNP, Smith, SSA, & SSB),ARUP Laboratories is a national reference laboratory and a worldwide leader in innovative laboratory research and development. ARUP offers an extensive test menu of highly complex and unique medical tests in clinical and anatomic pathology. Owned by the University of Utah, ARUP Laboratories client,medicine,medical supply,medical supplies,medical product
Introduction: Patients with systemic lupus erythematosus (SLE) are at increased risk of metabolic syndrome (MetS) and its complications. In absence of..
Systemic lupus erythematosus Bluish complexion, Bluish skin, Blue-tinge to the skin, Cough with cloudy, fishy-smelling mucus, Cough with cloudy, fishy-smelling sputum, , Chronic constipation (elderly people), , systemic lupus erythematosus
Here we demonstrate the use of redox labeled double- and single-stranded oligonucleotides as recognition probes for the reagentless, single-step, electrochemical detection of anti-DNA antibodies directly in blood serum.
Hi everyone. I am new here (and newly diagnosed) but wanted to share a little about myself. A year ago I had lumps on my neck, which after biopsy were thought to be a form of cutaneous lupus. My general practitioner ran a series of blood tests and my ANA was 1:640 anti-centromere. I went to a rheumatologist 2 weeks later who reran the blood work with a specialty lab - ANA was 1:2560. At this point I had no clinical symptoms and she told me it is possible I may never develop any. I had a baseline Echo and continued life as normal. A few weeks ago my life was turned upside-down. I began experiencing excruciating pain in my finger tips on my left hand. It felt like theyd been slammed in a car door and I could not use my hand at all. Went to general practitioner and then ER after 2 of them turned purple and would not change back to red. Both thought possibly Raynauds but it was not a classic presentation. Got in with rheumatologist again who confirmed Raynauds and explained that with scleroderma ...
RESULTS:Mean IgG-anti-IgA levels were significantly higher in psoriasis patients. The frequency of positive ANA testing was 21.1%; however, there was no correlation between IgG-anti-IgA antibody levels and ANA positivity. Only one patient had low IgA levels without high IgG-anti-IgA concentrations ...
Cardiac manifestations of NL include advanced condition defects and occasionally cardiomyopathy with a high morbidity and mortality rate. In this regard, a study by Ismirly et al. (3), which included 325 offspring exposed to maternal anti-SSA/Ro antibodies presenting with NL, showed a case fatality rate of 17%, with one-third of the cases dying in utero and a high post-natal mortality rate, too, because survival at 10 years for children born alive was ,85%, with the majority of deaths occurring within the first year of birth.. In this issue of the Journal, the same team, this time led by Saxena et al. (4), report performing a carefully conducted, complex study presenting the first evidence of maternal passively transferred autoimmunity. Subjects were identified by the U.S. Research Registry for Neonatal Lupus, which enrolled mothers with anti-SSA/Ro and/or SSB/La antibodies and having at least 1 child with NL. Maternal and cord blood in cases with cardiac NL or unaffected pregnancies were ...
A 62 year old woman was referred to hospital by her general practitioner because of a few days history of progressive shortness of breath. She had associated fever but no cough or sputum production. Her medical history included rheumatoid arthritis, for which she had been taking methotrexate for the past 12 years.. On examination, she was visibly dyspnoeic, with low oxygen saturations. Her respiratory rate was 22 breaths/min, oxygen saturation was 90% on air, heart rate was 100 beats/min, blood pressure was 115/72 mm Hg, and her temperature was 37.4ºC. Chronic hand changes, consistent with rheumatoid arthritis, were noted. Bilateral widespread crackles were heard on auscultation of the chest.. Blood tests showed a white blood cell count of 8.8×109/L (reference range 4-10) and a C reactive protein of 1219 nmol/L (,47.6). Further blood tests, including antinuclear antibody (ANA) and extractable nuclear antigen (ENA), were negative. Chest radiography showed diffuse alveolar shadowing throughout ...
Data Availability StatementAll the analysis reviews and email address details are on demand. confirmed by non-invasive liver investigations. Comprehensive recovery from the sufferers liver tests happened upon cessation from the medication. Triiodothyronine was a proper treatment alternative. Bottom line Levothyroxine-induced liver damage is a uncommon, and in todays case survey, a self-limiting, undesirable effect. The medical diagnosis of our affected individual was verified via non-invasive diagnostic methods. Understanding of this uncommon undesirable effect is essential in the differential medical diagnosis of sufferers whove commenced on levothyroxine and also have deranged liver organ enzymes in the framework of hypothyroidism. Alanine aminotransferase, Antinuclear antibody, Anti-hepatitis A pathogen antibody, 360A iodide Anti-hepatitis C pathogen antibody, Anti-hepatitis E pathogen antibody, Liver organ kidney microsome type 1 antibody, Alkaline phosphatase, Anti-smooth muscles ...
Antinuclear antibody[edit]. A test used to identify abnormal proteins, known as antinuclear antibodies, produced when the body ... "Antinuclear Antibody (ANA)". Retrieved 14 April 2020.. *^ "Complete blood count (CBC)". ... "Increasing Prevalence of Antinuclear Antibodies in the United States". Arthritis & Rheumatology. 72 (6): 1026-1035. doi:10.1002 ... "Association between Vitamin D Deficiency and Antinuclear Antibodies in Middle-Aged and Older U.S. Adults". Cancer Epidemiology ...
ANA) Antinuclear Antibodies". CARD in Libby, MT. Retrieved 2020-12-21. "LIBBY GROUND WATER CONTAMINATION Site Profile". United ...
Other auto-antibodies may be present: Antinuclear antibody measurements are not diagnostic for PBC because they are not ... Nesher G, Margalit R, Ashkenazi YJ (April 2001). "Anti-Nuclear Envelope Antibodies: Clinical Associations". Seminars in ... Anti-glycoprotein-210 antibodies, and to a lesser degree anti-p62 antibodies, correlate with the disease's progression toward ... Anti-gp210 antibodies are found in 47% of PBC patients. Anti-centromere antibodies often correlate with developing portal ...
"Antinuclear Antibody (ANA)". Retrieved 14 April 2020. "Complete blood count (CBC)". 19 ... A test used to identify abnormal proteins, known as antinuclear antibodies, produced when the body attacks its own tissues. It ... have shown an increase of antinuclear antibodies, a common biomarker for autoimmune diseases. This shows that there has been an ... "Increasing Prevalence of Antinuclear Antibodies in the United States". Arthritis & Rheumatology. 72 (6): 1026-1035. doi:10.1002 ...
Antinuclear antibody (ANA). *CBC with differential. *Chest X-ray. *Serum creatinine. *Urinalysis[11] ...
Ehrenstein MR (August 1999). "Antinuclear antibodies and lupus: causes and consequences". Rheumatology. 38 (8): 691-3. doi: ... I. A clinical analysis of 55 anti-Yo antibody-positive patients". Neurology. 42 (10): 1931-7. doi:10.1212/wnl.42.10.1931. PMID ... Franceschini F, Cavazzana I (February 2005). "Anti-Ro/SSA and La/SSB antibodies". Autoimmunity. 38 (1): 55-63. doi:10.1080/ ... "Possible Source of Kawasaki Disease Found". Wisnieski JJ, Naff GB (September 1989). "Serum IgG antibodies to C1q in ...
Subtypes of antinuclear antibodies include anti-Smith and anti-double stranded DNA (dsDNA) antibodies (which are linked to SLE ... was a part of an anti-nuclear antibody (ANA) reaction; the body produces antibodies against its own tissue. This discovery led ... Antinuclear antibody (ANA) testing and anti-extractable nuclear antigen (anti-ENA) form the mainstay of serologic testing for ... Antinuclear antibody test positive; sensitivity = 99%; specificity = 49%.[75]. *Immunologic disorder: Positive anti-Smith, anti ...
... pattern of anti-nuclear antibody, and complement-fixing antibodies to DNA in sera from patients with systemic lupus ... These are known as anti-nuclear antibodies (ANA) and have also been observed in concert with multiple sclerosis as part of ... Barned S, Goodman AD, Mattson DH (February 1995). "Frequency of anti-nuclear antibodies in multiple sclerosis". Primary. ... Antibodies to certain types of chromatin organization, in particular, nucleosomes, have been associated with a number of ...
In addition, he studied the relationship between juvenile idiopathic arthritis and uveitis to antinuclear antibodies (ANA), ... "Clinical correlates of antinuclear antibodies in juvenile rheumatoid arthritis". The Journal of Pediatrics. 83 (3): 386-389. ... His thesis was titled The significance of antibody affinity in immune complex disease. In 1976, Petty returned to Canada and ... the influence of maternal anti-IgA antibodies on the occurrence of IgA deficiency in the offspring. He and colleagues first ...
Of the antibodies, 90% have a detectable antinuclear antibody. Anticentromere antibody is more common in the limited form (80- ... and a normal antinuclear antibodies test result. No cure for scleroderma is known, though treatments exist for some of the ... It is most common in diffuse cutaneous scleroderma, and is often associated with antibodies against RNA polymerase (in 59% of ... The patient's white blood cells are destroyed with cyclophosphamide and rabbit antibodies against the white blood cells. Then, ...
... especially antinuclear antibodies (ANA). Around 80% of people with DM test positive for ANA and around 30% of people have ... anti-synthetase antibodies), including antibodies against histidine-tRNA ligase (also called Jo-1); antibodies to signal ... recognition particle (SRP); and anti-Mi-2 antibodies. Magnetic resonance imaging may be useful to guide muscle biopsy and to ... myositis-specific autoantibodies which include antibodies to aminoacyl-tRNA synthetases ( ...
... antinuclear antibodies were negative and p-ANCA was reactive. Coagulation studies were within normal limits. There was an ...
Antinuclear antibodies and anti-dsDNA antibodies may be positive. Biopsies show non specific inflammatory changes.[citation ...
... particularly antinuclear antibodies and rheumatoid factor". The Journal of Rheumatology. 20 (5): 900-3. PMID 8336322. Atta, AM ... It is defined as an antibody against the Fc portion of IgG and different RFs can recognize different parts of the IgG-Fc. RF ... Rheumatoid factor can also be a cryoglobulin (antibody that precipitates on cooling of a blood sample); it can be either type 2 ... Edkins A, Cushley W (2012). "The Jekyll and Hyde nature of antibodies". Biological Sciences Review. 25 (2): 4. Hermann, E; Vogt ...
... is an anti-nuclear antibody. Anti-Jo1 has been associated with inflammatory myopathies such as polymyositis and ... "A dermatomyositis and scleroderma overlap syndrome with a remarkable high titer of anti-exosome antibodies" (PDF). Reumatismo. ... and anti-Jo-1 antibodies--an interesting association". Clinical and Diagnostic Laboratory Immunology. 4 (2): 236-40. doi: ...
Subtypes of antinuclear antibodies include anti-Smith and anti-double stranded DNA (dsDNA) antibodies (which are linked to SLE ... Antinuclear antibody (ANA) testing and anti-extractable nuclear antigen (anti-ENA) form the mainstay of serologic testing for ... These are most commonly anti-nuclear antibodies and they result in inflammation. Diagnosis can be difficult and is based on a ... which leads to development of antinuclear antibodies. Monocytes isolated from whole blood of people with SLE show reduced ...
"Multiple nuclear dots antinuclear antibodies are not specific for primary biliary cirrhosis". Hepatology. 16 (1): 127-31. doi: ... antibodies to measles show expression in and localization to the nuclear bodies. In promyelocytic leukemia (PML), the oncogenic ...
Autoantibodies may be present including antinuclear, antiphospholipid, and anticardiolipin antibodies.[citation needed] The ...
Diagnostic testing includes screening for the positive antinuclear antibody.[citation needed] There is no current cure. The ... Vandergheynst F, Ocmant A, Sordet C, Humbel RL, Goetz J, Roufosse F, Cogan E, Sibilia J (2006). "Anti-pm/scl antibodies in ... Autoantibodies often found in these patients are the anti-PM/Scl (anti-exosome) antibodies. The symptoms that are seen most ... As of 2006 it is unclear which antibodies will best treat connective tissue diseases. One study from 2014 showed some potential ...
The antinuclear antibody (ANA) test is often ordered first. ANA is a marker of the autoimmune process - it is positive with a ... Antibodies are produced by B cells in two ways: (i) randomly, and (ii) in response to a foreign protein or substance within the ... An autoantibody is an antibody (a type of protein) produced by the immune system that is directed against one or more of the ... Initially, one B cell produces one specific kind of antibody. In either case, the B cell is allowed to proliferate or is killed ...
Graus F, Cordon-Cardo C, Posner JB (1985). "Neuronal antinuclear antibody in sensory neuronopathy from lung cancer". Neurology ... Nearly all people with the condition have anti-Hu antibodies in their serum. The antibody is produced by the body as an immune ... However, the injection of the antibodies into mice did not produce any disease, and the deposition of antibody was often not at ... Pohley I, Roesler K, Wittstock M, Bitsch A, Benecke R, Wolters A (2015). "NMDA-receptor antibody and anti-Hu antibody positive ...
Antinuclear antibodies are usually positive in drug-induced Lupus. Anti-Neutrophil Cytoplasmic antibodies (ANCA) can also be ... creating antinuclear antibodies leading to an immune response.[7] Further studies on the interactions between oxidants and ... Anti-histone antibodies in 95% of cases among those taking procainamide, hydralazine, chlorpromazine, and quinidine; however, ... Anti-Histone antibodies are positive in up to 95% of patients with drug induced lupus. The most common medications associated ...
Permin H, Wiik A (October 1978). "The prevalence of IgE antinuclear antibodies in rheumatoid arthritis and systemic lupus ... June 2010). "Antibodies specific for a segment of human membrane IgE deplete IgE-producing B cells in humanized mice". The ... In the second approach, antibodies specific for a domain of 52 amino acid residues, referred to as CεmX or M1' (M1 prime), ... Immunoglobulin E (IgE) is a type of antibody (or immunoglobulin (Ig) "isotype") that has been found only in mammals. IgE is ...
These are called anti-mitochondrial antibodies (AMA) and anti-nuclear antibodies (ANA), respectively. These antibodies are ... O'Brien C, Joshi S, Feld JJ, Guindi M, Dienes HP, Heathcote EJ (Aug 2008). "Long-term follow-up of antimitochondrial antibody- ... There is also evidence of anti-PDC-E2 antibodies in autoimmune hepatitis (AIH) patients. Pyruvate dehydrogenase deficiency (PDH ... "Catalytic domain of PDC-E2 contains epitopes recognized by antimitochondrial antibodies in primary biliary cirrhosis". World ...
The Anti-nuclear antibody may also be positive in up to 75% of patients. This subtype of arthritis behaves in a very similar ... Patients in this subtype are Rheumatoid factor negative; Anti-nuclear antibody is positive in approximately 25% of patients. ... as well as the presence of specific immune markers which may include Anti-nuclear antibody, HLA-B27, Rheumatoid factor and Anti ... The Anti-nuclear antigen (ANA) is positive in up to 80% of patients with oligoarthritis and is associated with a higher risk of ...
In certain patients, anti-nuclear antibody may play a role. The optimal treatment is prevention. Rigorous and continuous ... Rashid RM, Hauck M, Lasley M (Nov 2008). "Anti-nuclear antibody: a potential predictor of calciphylaxis in non-dialysis ...
Other genes in the region, C4A-null and TNF may associated with autoimmune hepatitis The appearance of anti-nuclear antibodies ... Christian N, Smikle MF, DeCeulaer K, Daniels L, Walravens MJ, Barton EN (March 2007). "Antinuclear antibodies and HLA class II ... of these half had anti-transglutaminase antibodies, but few had endomysial antibody. This could indicate an association with ... DR3 is found to correlate with anti-Ro/La antibodies in SLE. HLA-DR3 has been consistently observed at high frequencies in ...
These might include: rheumatoid factor, antinuclear factor (ANF), extractable nuclear antigen, and specific antibodies. ... The drugs to treat rheumatoid arthritis (RA) range from corticosteroids to monoclonal antibodies given intravenously. Due to ...
Rheumatoid factor, cyclic citrullinated peptide antibodies and antinuclear antibodies are usually negative in IH. Palindromic ... 2007). "An unexpectedly high frequency of MEFV mutations in patients with anti-citrullinated protein antibody-negative ...
The presence of anti-glomerular basement membrane (GBM) antibodies suggests type I RPGN; antinuclear antibodies (ANA) may ... It is thought that antineutrophil cytoplasmic antibodies (ANCA) interact with antigens in the cytoplasm of neutrophils to cause ... The majority of type I disease, however, features anti-GBM antibodies alone; these cases are considered idiopathic. ... of RPGN and features neither immune complex deposition nor anti-GBM antibodies. Instead, the glomeruli are damaged in an ...
Anticardiolipin antibodies[edit]. Anti-cardiolipin antibodies can be detected using an enzyme-linked immunosorbent assay (ELISA ... In APS there are also antibodies binding to Protein S, which is a co-factor of protein C. Thus, anti-protein S antibodies ... Thus, anti-annexin A5 antibodies increase phospholipid-dependent coagulation steps.[8] The Lupus anticoagulant antibodies are ... Antiphospholipid syndrome is an autoimmune disease, in which "antiphospholipid antibodies" (anticardiolipin antibodies and ...
Targeting can also be achieved by attaching the radioisotope to another molecule or antibody to guide it to the target tissue. ... History of the anti-nuclear movement. *International Day against Nuclear Tests. *Nuclear close calls ... CD20 monoclonal antibody conjugated to yttrium-90.[75] In 2003, the FDA approved the tositumomab/iodine (131I) tositumomab ... regimen (Bexxar), which is a combination of an iodine-131 labelled and an unlabelled anti-CD20 monoclonal antibody.[76] These ...
Permin H, Wiik A (1978). "The prevalence of IgE antinuclear antibodies in rheumatoid arthritis and systemic lupus erythematosus ... "Antibody structure". Archived from the original on September 6, 2008.. *^ Erb KJ (2007). "Helminths, allergic disorders and IgE ... Chang TW, Wu PC, Hsu CL, Hung AF (2007). Anti-IgE antibodies for the treatment of IgE-mediated allergic diseases. Adv. Immunol ... Immunoglobulin E (IgE) is a type of antibody (or immunoglobulin (Ig) "isotype") that has only been found in mammals. IgE is ...
Antinuclear antibodies (ANAs, also known as antinuclear factor or ANF)[1] are autoantibodies that bind to contents of the cell ... anti-Sm antibodies, anti-nRNP antibodies, anti-Scl-70 antibodies, anti-dsDNA antibodies, anti-histone antibodies, antibodies to ... Anti-nuclear ribonucleoprotein (anti-nRNP) antibodies, also known as anti-U1-RNP antibodies, are found in 30-40% of SLE. They ... This pattern is associated with anti-dsDNA antibodies, antibodies to nucleosomal components, and anti-histone antibodies. There ...
"One of the most common tests looks for antinuclear antibodies (ANA), and antibodies to double-stranded DNA (which makes up ... "One of the most common tests looks for antinuclear antibodies (ANA), and antibodies to double-stranded DNA (which makes up ... because most XL-CGD carrier females who have been tested for lupus do not have the auto-antibodies used to diagnose lupus or, ... these symptoms as lupus-like because most XL-CGD carrier females who have been tested for lupus do not have the auto-antibodies ...
In the 1990s and 2000s the roles of antibodies in the condition became more clear. SPS patients generally have GAD antibodies, ... Anti-thyroid, anti-intrinsic factor, anti-nuclear, anti-RNP, and anti-gliadin are also often present in blood tests. ... It is also unknown whether these antibodies are pathogenic.[25] The amount of GAD antibody titers found in SPS patients does ... Most SPS patients with high-titer GAD antibodies also have antibodies that inhibit GABA-receptor-associated protein (GABARAP).[ ...
Pattern of Antinuclear Antibody, and Complement-Fixing Antibodies to DNA in Sera from Patients with Systemic Lupus ... S Barned, AD Goodman, DH Mattson (1995). "Frequency of anti-nuclear antibodies in multiple sclerosis". Neurology. 45 (2): 384- ...
Antinuclear antibody (ANA) test can detect an underlying connective tissue disorder, especially SLE ... Antineutrophil cytoplasmic antibody (ANCA) may highly suggest granulomatosis with polyangiitis, microscopic polyangiitis, ... A systematic review of antineutrophil cytoplasmic antibody (ANCA) positive vasculitis identified best treatments depending on ... Bosch X, Guilabert A, Espinosa G, Mirapeix E (2007). "Treatment of antineutrophil cytoplasmic antibody associated vasculitis: a ...
... mimetic[edit]. Antibody mimetics are organic compounds that, like antibodies, can specifically bind antigens. They are ... Asymmetrical antibodies[edit]. Heterodimeric antibodies, which are also asymmetrical and antibodies, allow for greater ... How Lymphocytes Produce Antibody from Cells Alive!. *Antibody applications Fluorescent antibody image library, University of ... Nucleic acids and small molecules are sometimes considered antibody mimetics, but not artificial antibodies, antibody fragments ...
A number of specific antibodies found in the blood (antinuclear antibody (ANA), anti-smooth muscle antibody (SMA), anti-liver ... Positive antibodies against soluble liver antigen[14] (this group behaves like group 1)[15] (anti-SLA, anti-LP) ... The presence of anti-mitochondrial antibody is more suggestive of primary biliary cholangitis.[8] ... and anti-mitochondrial antibody (AMA)) are of use, as is finding an increased immunoglobulin G level. ...
Other auto-antibodies may be present:. Antinuclear antibody measurements are not diagnostic for PBC because they are not ... Anti-gp210 antibodies are found in 47 percent of PBC patients.[29][30]. Anti-centromere antibodies often correlate with ... Nesher G, Margalit R, Ashkenazi YJ (April 2001). "Anti-Nuclear Envelope Antibodies: Clinical Associations". Seminars in ... Anti-glycoprotein-210 antibodies, and to a lesser degree anti-p62 antibodies, correlate with the disease's progression toward ...
... pattern of anti-nuclear antibody, and complement-fixing antibodies to DNA in sera from patients with systemic lupus ... Barned S, Goodman AD, Mattson DH (February 1995). "Frequency of anti-nuclear antibodies in multiple sclerosis". Neurology. 45 ( ... These are known as anti-nuclear antibodies (ANA) and have also been observed in concert with multiple sclerosis as part of ... Antibodies to certain types of chromatin organization, in particular, nucleosomes, have been associated with a number of ...
Anti-nuclear antibody. *PBC: *Anti-gp210. *Anti-p62. *Anti-sp100. *ENA: *Anti-topoisomerase/Scl-70 ... Approximately only 0.1 to 0.5 percent of the general population has the antibody.[citation needed] ... Franceschini F, Cavazzana I (February 2005). "Anti-Ro/SSA and La/SSB antibodies". Autoimmunity. 38 (1): 55-63. doi:10.1080/ ... Immunofluorescence pattern of SS-A and SS-B antibodies. Produced using serum from a patient on HEp-20-10 cells with a FITC ...
... anti-nuclear antibodies, rheumatoid factor, HLA-B27, uric acid, or Lyme disease antibodies may also be obtained.[5] ...
Anti-nuclear antibodies) ... About two-thirds are associated with auto-antibodies to ... No immune deposits can be seen on staining, however blood tests may be positive for the ANCA antibody.[4]:558-559 ... Diagnosis may be made on clinical findings or through antistreptolysin O antibodies found in the blood. A biopsy is seldom done ... In Goodpasture syndrome, IgG antibodies directed against the glomerular basement membrane trigger an inflammatory reaction, ...
... and antinuclear antibodies. Affected individuals may experience systemic organ involvement. No single test for scleroderma ... Monoclonal antibody to inhibit T lymphocyte activation by binding to CD2 portion of human leukocyte function antigen-3.. IM. B ... Monoclonal antibody against CD20, which is expressed on B lymphocytes. IV. C (Au). Infusion-related reactions, infection, ... Anti-topoisomerase 1 antibodies, in turn, stimulate type I interferon production.. *Fibroblasts are recruited and activated by ...
Antibodies -- Antibody -- Anticholinergic -- Anticholinergic agent -- Anticholinergic agents -- Anticholinergics -- Antichrist ... Antinuclear movement -- Antioch -- Antiochus -- Antiochus I -- Antiochus III -- Antiochus IV -- Antiope -- Antioquia -- ...
... the presence of anti-phospholipid antibodies, and antinuclear antibodies. Anti-phospholipid antibodies are targeted toward the ... These antibodies also jeopardize the health of the uterus by altering the blood flow to the uterus. Antinuclear antibodies ... Both the presence of anti-phospholipids antibodies and antinuclear antibodies have toxic effects on the implantation of embryos ... Rh disease is caused by the mother producing antibodies (including IgG antibodies) against the Rhesus D antigen on her baby's ...
Speckled Immunofluorescence staining pattern of antinuclear antibodies on HEp-20-10 cells ... such as antinuclear antibody (ANA) and rheumatoid factor (because SS frequently occurs secondary to rheumatoid arthritis), ... Among the complications discussed above, women with anti-Ro/SS-A and anti-La/SS-B antibodies who become pregnant, have an ... Blood tests can be done to determine if a patient has high levels of antibodies that are indicative of the condition, ...
... as abnormal antinuclear antibody and rheumatoid factor can be found in the serum of more than 50% of persons with BMS, but ...
Anti-Nuclear Anti-body. *(a) Australian National Airways. *Australian Natives' Association. *ANC - (i) African National ...
Antinuclear antibody reflexive panel, rheumatoid factor, and antiphospholipid antibodies are tests that may assist in the ... Patients often have cartilage-specific antibodies present during acute relapsing polychondritis episodes. ...
Distinguishing laboratory characteristics are a positive, speckled anti-nuclear antibody and an anti-U1-RNP antibody.[14][15] ... The universal serological findings in patients with MCTD is the presence of anti-nuclear antibody, with anti-nRNP specificity, ... If the patient has edematous hands and/or swollen fingers in conjunction with elevated titers of antinuclear antibodies, an ... The specific signs to suspect this disease is the presence of positive antinuclear antibodies (ANA), specifically anti-RNP, ...
... a type of anti-nuclear antibody. Anti-nuclear antibodies are a common feature in SLE and anti-dsDNA antibodies are highly ... "Guidelines for clinical use of the antinuclear antibody test and tests for specific autoantibodies to nuclear antigens. ... The kinetoplast found in C.luciliae allows them to be used for the detection of anti-dsDNA antibodies, ... "Immunofluorescence staining pattern of anti-dsDNA antibodies on C. luciliae substrate. The kinetoplast located near the ...
Of the antibodies, 90% have a detectable anti-nuclear antibody. Anti-centromere antibody is more common in the limited form (80 ... The patient's white blood cells are destroyed with cyclophosphamide and rabbit antibodies against the white blood cells. Then ... and a normal antinuclear antibodies test result.[38] ... and is often associated with antibodies against RNA polymerase ... anti-topoisomerase antibodies) and occasionally by biopsy. ...
Antinuclear antibodies (ANAs, also known as antinuclear factor or ANF)[1] are autoantibodies that bind to contents of the cell ... anti-Sm antibodies, anti-nRNP antibodies, anti-Scl-70 antibodies, anti-dsDNA antibodies, anti-histone antibodies, antibodies to ... Anti-nuclear ribonucleoprotein (anti-nRNP) antibodies, also known as anti-U1-RNP antibodies, are found in 30-40% of SLE. They ... This pattern is associated with anti-dsDNA antibodies, antibodies to nucleosomal components, and anti-histone antibodies. There ...
Source for information on Antinuclear Antibody Test: Gale Encyclopedia of Medicine, 3rd ed. dictionary. ... Antinuclear Antibody Test Definition The antinuclear antibody (ANA) test is a test done early in the evaluation of a person for ... Antinuclear Antibody Test. Definition. The antinuclear antibody (ANA) test is a test done early in the evaluation of a person ... Autoantibody- An antibody that attacks the bodys own cells or tissues.. Antinuclear antibodies- Autoantibodies that attack ...
Antinuclear antibodies (ANA) are autoantibodies directed against the nuclei of some of ones own body cells. They are present ... What are Antinuclear Antibodies?. News-Medical. ( ... In IF-ANA the bound antibodies are detected using another antibody which binds to human antibody molecules, and which carries a ... In ELISA, the antigen-antibody complexes are detected by another antibody which binds to human antibodies, but which carries an ...
An antinuclear antibody test checks to see if you have an autoimmune disorder, a condition where the immune system attacks ... antinuclear antibody) test?. An ANA test looks for antinuclear antibodies in your blood. If the test finds antinuclear ... ANA (Antinuclear Antibody) Test. ... But an antinuclear antibody attacks your own healthy cells instead. Its called "antinuclear" because it targets the nucleus ( ...
Tagged asaccuracyANAantinuclear antibody testsautoantibodiesautoimmune disordersdiagnostic testsjuvenile arthritisscleroderma ... he may arrange to have you take an antinuclear antibody (ANA) test. In these kinds of connective-tissue diseases, the immune- ... So, while a positive result for double-stranded DNA and SM antibodies may confirm SLE, a negative test doesnt necessarily rule ... Healthy people can also show a positive result: around 2 per cent of the population have mildly elevated antibodies without ...
Anti-nuclear antibody?. deleted_user 09/26/2009. Okay my blood work has not been explained by a doctor yet, but I have a copy ...
What is an antinuclear antibody panel?. Antibodies are proteins made by your immune system. They help your body recognize and ... When is an antinuclear antibody panel needed?. Your doctor will likely order an ANA panel if you have signs or symptoms of an ... Antinuclear Antibody Panel (ANA Test). Medically reviewed by Debra Sullivan, PhD, MSN, RN, CNE, COI on August 30, 2017. - ... Antibodies that attack healthy proteins within the nucleus - the control center of your cells - are called antinuclear ...
... he may arrange to have you take an antinuclear antibody (ANA) test. ... he may arrange to have you take an antinuclear antibody (ANA) test. In these kinds of connective-tissue diseases, the immune- ... So, while a positive result for double-stranded DNA and SM antibodies may confirm SLE, a negative test doesnt necessarily rule ... Healthy people can also show a positive result: around 2 per cent of the population have mildly elevated antibodies without ...
According to the presence of antinuclear antibodies (ANA) at baseline and during the f... ... Antinuclear antibodies and response to IFNbeta-1a therapy in relapsing-remitting multiple sclerosis.. *Ciccarelli O ... According to the presence of antinuclear antibodies (ANA) at baseline and during the first 6 months of treatment, patients were ...
Antinuclear antibodies are the markers used for connective tissue diseases. Aim. To determine antinuclear antibody frequency ... Antinuclear antibody (ANA) is the brand name of the antibodies towards nuclear and cytoplasmic structures of the cell. ANA is a ... The Prevalence of Antinuclear Antibodies in Patients with Sarcoidosis. Senol Kobak,1 Hatice Yilmaz,1 Fidan Sever,2 Arzu Duran,3 ... anti-Ro antibody in one patient, anti-Scl-70 antibody in one patient, and anti-dsDNA antibody in one patient, and eight ...
Antinuclear antibodies: An overview. In: Wallace DJ, Hahn BH, eds. Dubois Lupus Erythematosus. 5th ed. Baltimore, Md: Williams ... Antinuclear Antibodies (ANA) Profile, 11 biomarkers, by Multiplex Immunoassay, dsDNA, RNP, Sm, SS-A, SS-B, Scl-70, Chromatin, ... Antichromatin Antibodies. AI. 51775-5. 164920. ANA Comprehensive Plus Profile. 012701. Antiribosomal P Antibodies. AI. 13636-6 ... Detection of antinuclear antibodies: Comparative evaluation of enzyme immunoassay and indirect immunofluorescence methods. Arch ...
Range of antinuclear antibodies in "healthy" individuals Arthritis Rheum. 1997 Sep;40(9):1601-11. doi: 10.1002/art.1780400909. ... Objective: To determine the range of antinuclear antibodies (ANA) in "healthy" individuals compared with that in patients with ...
... test results for antibodies to most nuclear antigen subsets are negative. ... encoded search term (What is the significance of antinuclear antibodies (ANAs) in rheumatoid arthritis (RA)?) and What is the ... What is the significance of antinuclear antibodies (ANAs) in rheumatoid arthritis (RA)?. Updated: Feb 07, 2020 ... Antibodies to porphyromonas gingivalis are associated with anticitrullinated protein antibodies in patients with rheumatoid ...
Accurate Interpretation of Antinuclear Antibodies Test Key to Confirming Autoimmune Disease. New Research Finds Pattern for ... The presence of antinuclear antibodies (ANA) indicates the possibility of autoimmunity and the indirect immunofluorescence (IIF ...
The antinuclear antibody panel is a blood test that looks at antinuclear antibodies (ANA). ... Antinuclear antibodies (ANA). ... The antinuclear antibody panel is a blood test that looks at antinuclear antibodies (ANA). ... The antinuclear antibody test looks for antibodies that bind to a part of the cell called the nucleus. ...
Find Antinuclear antibody panel information, treatments for Antinuclear antibody panel and Antinuclear antibody panel symptoms. ... MedHelps Antinuclear antibody panel Center for Information, Symptoms, Resources, Treatments and Tools for Antinuclear antibody ... Posts on Antinuclear antibody panel (53757). Negative ANA, Other Symptoms of Lupus/Autoimmune disorders - Autoimmune Disorders ...
ANTI-NUCLEAR ANTIBODY (ANA) Synonym(s):. Synonym(s). ​Anti ANA, Antinuclear Antibody, Anti Nuclear Antibody, ANA, ANF ...
Three screening approaches that use antinuclear antibody and specific antibody tests are discussed. ... Table 4. Tests to Consider When Antinuclear Antibody is Positive and Multiplex 11-Antibody Test is Negativea ... Laboratory tests include relatively nonspecific antinuclear antibody (ANA) testing and/or tests for individual antibodies that ... Antinuclear Antibodies (ANA). ...
Mouse monoclonal Nuclear Matrix Protein p84 antibody [3121C2a] validated for WB, Dot and tested in Human. Immunogen ... Anti-Nuclear Matrix Protein p84 antibody [3121C2a]. See all Nuclear Matrix Protein p84 primary antibodies. ... Primary antibodies. Secondary antibodies. ELISA, Matched Antibody Pairs and Multiplex Immunoassays. Cell and tissue imaging ... Anti-Nuclear Matrix Protein p84 antibody [3121C2a] (ab54370) + immunising recombinant fragment. Predicted band size: 76 kDa. ...
... Test Overview. An antinuclear antibody (ANA) test measures the amount and pattern of antibodies ... An antinuclear antibody (ANA) test measures the amount and pattern of antibodies in your blood that work against your own body ... An antinuclear antibodies (ANA) test is done to help identify problems with the immune system, such as:. *Rheumatoid arthritis. ... When a person has an autoimmune disease, the immune system produces antibodies that attach to the bodys own cells as though ...
A fiberoptic evanescent-wave sensor has been developed for the measurement of antinuclear antibodies in sera from patients and ... Antinuclear antibodies Fiberoptic biosensor Localized surface plasmon resonance Gold nanoparticles Enzyme-linked immunosorbent ... Detection of antinuclear antibodies by a colloidal gold modified optical fiber: comparison with ELISA. ... A fiberoptic evanescent-wave sensor has been developed for the measurement of antinuclear antibodies in sera from patients and ...
Sometimes antibodies are made in response to healthy cells. Antinuclear antibodies are antibodies made against antigens ( ... This test measures the level of antinuclear antibodies. Antibodies are made by the body to fight infection or other "foreign" ... Sample Result: Antinuclear Antibodies (ANA). This browser does not support inline PDFs. To view the Sample Results file, click ... T3 Total and Thyroid Peroxidase Antibody (TPO). ...
... test measures the amount and pattern of antibodies in your blood that work against your own body (autoimmune reaction). The ... An antinuclear antibody (ANA) test measures the amount and pattern of antibodies in your blood that work against your own body ... An antinuclear antibody (ANA) test measures the amount and pattern of antibodies in your blood that work against your own body ... An antinuclear antibodies (ANA) test is done to help identify problems with the immune system, such as:. *Rheumatoid arthritis. ...
Antinuclear Antibody (ANA). Accessed 2/11/2019.. *Lupus Foundation of America. The antinuclear antibody (ANA) test. Accessed 2/ ... How is an antinuclear antibody test used?. An ANA test is usually not performed as part of a routine physician visit. Instead, ... Antinuclear Antibodies (ANA). Accessed 2/11/2019.. *Malleson NP, Mackinnon MJ, Sailer-Hoeck, et. al. Review for the generalist ... How is a test for antinuclear antibody performed?. Because ANAs can be present in various situations, the ANA test is not used ...
What is Antinuclear antibodies? Meaning of Antinuclear antibodies medical term. What does Antinuclear antibodies mean? ... Looking for online definition of Antinuclear antibodies in the Medical Dictionary? Antinuclear antibodies explanation free. ... Related to Antinuclear antibodies: Lupus. Antinuclear antibodies. Autoantibodies that attack substances found in the center, or ... Antinuclear antibodies (ANA) and anti-double stranded DNA (dsDNA) antibodies are important antibodies determinants in the ...
Rabbit polyclonal Nuclear Receptor Corepressor NCoR antibody validated for WB, IP, IHC, ChIP, ICC/IF and tested in Human and ... Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade. See all Nuclear Receptor Corepressor NCoR primary antibodies. ... Primary antibodies. Secondary antibodies. ELISA, Matched Antibody Pairs and Multiplex Immunoassays. Cell and tissue imaging ... Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP ...
Anti-nuclear antibody production and immune-complex glomerulonephritis in BALB/c mice treated with pristane. M Satoh, A Kumar, ... Anti-nuclear antibody production and immune-complex glomerulonephritis in BALB/c mice treated with pristane ... Anti-nuclear antibody production and immune-complex glomerulonephritis in BALB/c mice treated with pristane ... Anti-nuclear antibody production and immune-complex glomerulonephritis in BALB/c mice treated with pristane ...
Understanding antinuclear antibodies You will receive an email whenever this article is corrected, updated, or cited in the ... Understanding antinuclear antibodies. The Journal of the American Osteopathic Association, June 1991, Vol. 91, 563. doi:10.7556 ... Pertusi R, Rubin B. Understanding antinuclear antibodies. J Am Osteopath Assoc 1991;91(6):563. doi: 10.7556/jaoa.1991.91.6.563. ... diagnostic criteria for connective tissue disorders frequently include positive antinuclear antibody (ANA) assays. Proper ...
We ask about your Anti-Nuclear Antibody level in order to learn more about your chemistry-related lab values. ... What Causes Elevated Anti-Nuclear Antibody ANA Level?. Elevated anti-nuclear antibody ANA level can have various causes, ... A differential diagnosis of your symptoms and risk factors finds the likely cause of elevated anti-nuclear antibody ANA level: ... We begin by identifying the disease conditions which have "elevated anti-nuclear antibody ANA level" as a symptom. Here are ...
  • Antinuclear antibodies ( ANAs , also known as antinuclear factor or ANF ) [1] are autoantibodies that bind to contents of the cell nucleus . (
  • Autoantibodies are proteins built by the body, but instead of guarding against foreign material (including bacteria, viruses, and fungi) as normal antibodies do, they attack the body's own cells. (
  • The antinuclear antibody test looks for a group of autoantibodies that attack substances found in the center (nucleus) of all cells. (
  • Antinuclear antibodies - Autoantibodies that attack substances found in the center, or nucleus, of all cells. (
  • Antinuclear antibodies (ANA) are autoantibodies directed against the nuclei of some of one's own body cells. (
  • Guidelines for clinical use of the antinuclear antibody test and tests for specific autoantibodies to nuclear antigens. (
  • Antinuclear antibodies (ANA) are autoantibodies mainly located in the nucleus of affected cells. (
  • Additionally, many patients with morphea have antinuclear antibody and rheumatoid factor autoantibodies. (
  • Autoantibodies are types of antibodies that target and react to your own body (called an autoimmune reaction). (
  • Screening for IgG Antinuclear Autoantibodies by HEp-2 Indirect Fluorescent Antibody Assays and the Need for Standardization. (
  • The detection of the autoantibodies present in the blood serum of an individual is known as antinuclear antibody Test. (
  • ANA stands for antinuclear antibodies or autoantibodies . (
  • Antibodies that are directed against one's own tissues are referred to as autoantibodies. (
  • Autoimmune diseases are conditions characterized by a disorder of the immune system featured by the abnormal production of antibodies (autoantibodies) directed against the tissues of the body. (
  • an·ti·nu·cle·ar n(y)ü klē ər adj being antibodies or autoantibodies that react with components and esp. (
  • Laboratory studies can show leukopenia, neutropenia (including agranulocytosis), elevated erythrocyte sedimentation rate, normal coagulation studies, and positive autoantibodies including p- and cANCA, anti-nuclear antibody , and lupus anticoagulant. (
  • When these antibodies make incorrect calls, identifying a naturally-occurring protein (or self protein) as foreign, they are called autoantibodies. (
  • These antibodies are called autoantibodies. (
  • Antibodies produced in an autoimmune disease are called autoantibodies. (
  • Antinuclear antibodies (ANA) are autoantibodies that are produced against protein markers present in the nucleus of certain cells of the body. (
  • There are many subtypes of ANAs such as anti-Ro antibodies , anti-La antibodies , anti-Sm antibodies , anti-nRNP antibodies , anti-Scl-70 antibodies , anti-dsDNA antibodies , anti-histone antibodies , antibodies to nuclear pore complexes, anti-centromere antibodies and anti-sp100 antibodies . (
  • What is the significance of antinuclear antibodies (ANAs) in rheumatoid arthritis (RA)? (
  • Although ANAs are present in approximately 40% of patients with RA, test results for antibodies to most nuclear antigen subsets are negative. (
  • Standardisation of the quantitative determination of antinuclear antibodies (ANAs) with a homogeneous pattern. (
  • The variety of ANAs used for the treatment include anti-La antibodies, anti-RNP antibodies, anti-Ro antibodies, anti-dsDNA antibodies, anti-Scl-70 antibodies, anti-centromere antibodies, anti-sp100 antibodies, and anti-histone antibodies. (
  • Antinuclear antibodies (ANAs) are found in patients whose immune system is predisposed to cause inflammation against their own body tissues. (
  • Antinuclear antibodies (ANAs) indicate the possible presence of autoimmunity. (
  • Anti-nuclear antibody - Anti nuclear antibodies (ANAs, also known as anti nuclear factor or ANF ) are antibodies directed against contents of the cell nucleus. (
  • Autoantibody - An antibody that attacks the body's own cells or tissues. (
  • A cascade autoantibody testing is usually performed by employing antinuclear antibodies (ANA) test as a first screening test and the other tests as second level determinations. (
  • To study the predictive value of antinuclear autoantibody (ANA) tests and antihistone antibodies (AHA) as risk factors for development of chronic asymptomatic uveitis of insidious onset in juvenile idiopathic arthritis (JIA). (
  • Arguably one of the most commonly ordered autoantibody tests, the antinuclear antibody (ANA) test is frequently used in a variety of medical disciplines including rheumatology. (
  • This test is also called the fluorescent antinuclear antibody test or FANA. (
  • Laboratories often use ELISA as a first screening, but will follow up with another test called the Fluorescent Antinuclear Antibody (FANA) test to confirm the results. (
  • The fluorescent antinuclear antibody test (FANA) was designed by George Friou, M.D. in 1957. (
  • The American College of Rheumatology (formerly the American Rheumatism Association) diagnostic criteria for connective tissue disorders frequently include positive antinuclear antibody (ANA) assays. (
  • Following the recent statement by the American College of Rheumatology that the IIF technique should be considered as the standard screening method for the detection of anti-nuclear antibodies (ANA), the biomedical industry has developed technological solutions which might significantly improve automation of the procedure, not only in the preparation of substrates and slides, but also in microscope reading. (
  • The best, and most common (recommended by the American College of Rheumatology) is the "indirect immunofluorescence test," which reports results in titers (concentrations of antibody). (
  • The antinuclear antibody (ANA) test is a test done early in the evaluation of a person for autoimmune or rheumatic disease, particularly systemic lupus erythematosus (SLE). (
  • Other diseases, such as scleroderma, Sj ö gren's syndrome, Raynaud's disease, rheumatoid arthritis, and autoimmune hepatitis, often have a positive test for antinuclear antibodies. (
  • The antinuclear antibody test is done by adding a person's serum to commercial cells mounted on a microscope slide. (
  • No special preparations or diet changes are required before a person undergoes an antinuclear antibody test. (
  • A positive ANA test means that the antibodies were detected in the individual's blood sample. (
  • What is an ANA (antinuclear antibody) test? (
  • An ANA test looks for antinuclear antibodies in your blood. (
  • If the test finds antinuclear antibodies in your blood, it may mean you have an autoimmune disorder . (
  • A positive result on an ANA test means that antinuclear antibodies were found in your blood. (
  • If your doctor suspects that you have an autoimmune disorder such as systemic lupus erythematosus (SLE), rheumatoid arthritis or scleroderma, he may arrange to have you take an antinuclear antibody (ANA) test. (
  • The ANA test result is reported as a titre - a measure of how much the blood sample can be diluted and yet still show the presence of antibodies. (
  • Some people with autoimmune diseases may get a negative test result for ANA but positive for other antibodies. (
  • In the subgroup analysis made by immunblot test, one patient had anticentromere antibody, one had anti-Ro antibody, one had anti-Scl-70 antibody, one had anti-dsDNA antibody, and eight patients were negative. (
  • Test is intended to be used as a follow-up to Antinuclear Antibodies (ANA) Direct [164855] . (
  • The presence of antinuclear antibodies (ANA) indicates the possibility of autoimmunity and the indirect immunofluorescence (IIF) assay on HEp-2 cells is the standard blood test (ANA-HEp-2) used to detect ANA. (
  • The antinuclear antibody panel is a blood test that looks at antinuclear antibodies (ANA). (
  • The antinuclear antibody test looks for antibodies that bind to a part of the cell called the nucleus. (
  • If the test is positive, a panel of tests may be done to identify specific antibodies. (
  • An antinuclear antibody (ANA) test measures the amount and pattern of antibodies in your blood that work against your own body (autoimmune reaction). (
  • If there are more antibodies in the blood than normal, the test is positive. (
  • This test measures the level of antinuclear antibodies. (
  • How is a test for antinuclear antibody performed? (
  • In this test, the person's blood sample is mixed with antigens (portions of proteins that bind antibodies). (
  • If the antibody for that antigen is in the blood, the test can find it. (
  • How is an antinuclear antibody test used? (
  • Review for the generalist: The antinuclear antibody test in children - When to use it and what to do with a positive titer. (
  • The antinuclear antibodies (ANA) test has been a cornerstone of the evaluation of connective tissue disease. (
  • In conclusion, although a negative antinuclear antibodies test makes a diagnosis of lupus serositis unlikely, high antinuclear antibodies titres in pleural or pericardial fluid are not diagnostic of lupus serositis even when as high as 1:5,120. (
  • Tests for anti-dsDNA, anti-RNP, anti-Sm antibodies, and a screening test for systemic rheumatic diseases (ANA Rheuma Screen) were carried out using Varelisa Enzyme Immunoassays (Pharmacia and Upjohn). (
  • article{OSullivan2013AntinuclearAT, title={Antinuclear antibody test. (
  • The antinuclear antibody (ANA) test is widely used as a serological marker of autoimmune disease. (
  • Antinuclear antibodies: when to test and how to interpret findings. (
  • The clinical utility of a positive antinuclear antibody test result. (
  • 2 Herein we report a patient whose male gender, young age, unusual presentation and negative antinuclear antibody (ANA) test created some diagnostic difficulties. (
  • Anti-nuclear Antibody (ANA) Test is used to detect antinuclear antibodies in blood. (
  • An antinuclear antibody (ANA) test is a type of blood test. (
  • Conclusion: This study, which followed up on the subjects who had disparate ANA and ENA test results, showed that anti-ENA antibodies may exist years earlier than ANA. (
  • The increasing use of Antinuclear Antibody Test can be attributed to the growing number of autoimmune diseases, rising disposable incomes, increasing awareness about the symptoms, complications and diagnosis of such disease, and the growing demand for Antinuclear Antibody Test majorly due to increase in the number of diabetic populace having type I diabetes. (
  • Brazil accounts for the largest share of the Latin America Antinuclear Antibody Test market. (
  • The demand for Antinuclear Antibody Test is expected to further increase in the light of increasing awareness and the increasing demand for the same. (
  • The major market contributors for the Latin America Antinuclear Antibody Test market are Zeus Scientific Inc., Inova Diagnostics, Immuno Concepts , Euroimmun AG, Antibodies Inc., Thermo Fisher Scientific inc. (
  • Are Antinuclear Antibody Test Results Accurate? (
  • You are referring to the antinuclear antibody test (ANA), a test which is positive often in the disease systemic lupus erythematosus (SLE), or lupus. (
  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. (
  • Antinuclear antibodies (medical test): Type of Antibody tests . (
  • The direct antiglobulin (Coombs') test was positive in all 7 cats evaluated, whereas the serum antinuclear antibody titer was greater than or equal to 1:10 in 5 of the 8 cats tested. (
  • Antinuclear antibody test - A test for unusual antibodies that are directed against structures within the nucleus of the cell. (
  • The test finds small amounts of these antibodies in up to 15% of healthy people. (
  • About 95% of people with this autoimmune disease test positive for antinuclear antibodies. (
  • Many conditions can trigger a positive antinuclear antibody test even without an autoimmune disease. (
  • The antinuclear antibody (ANA) test is used to evaluate for underlying autoimmune disorder. (
  • The systemic autoimmune rheumatic diseases (SARDs) that are frequently associated with a positive anti-nuclear antibody (ANA) test result include systemic lupus erythematosus (SLE), Sjögren's syndrome (SS), systemic sclerosis (SSc), dermatomyositis (DM) and mixed connective tissue disease (MCTD). (
  • People with autoimmune disorders can have a variety of symptoms that are vague and non-specific and that change over time, progressively worsen.The antinuclear antibody (ANA) test is used as a primary test to help evaluate a person for autoimmune disorders that affect many tissues and organs throughout the body (systemic) and is most often used as one of the tests to help diagnose systemic lupus erythematosus (SLE). (
  • The antinuclear antibody (ANA) test is a standard screening assay for detecting multiple antibodies that may be produced by a patient with an autoimmune or ANA associated rheumatic disease (AARD). (
  • The Antinuclear Antibody Test screens for the presence of antinuclear antibodies (ANA) in blood. (
  • Antinuclear antibody (ANA) test screens for the presence of ANA in blood. (
  • The Antinuclear Antibody Test can be performed either by Immunoassay method (ELISA) or Indirect Fluorescent Antibody (IFA) method to detect ANA in blood. (
  • The Antinuclear Antibody Test can be affected by a number of factors including: · Certain medications like procainamide, hydralazine, phenytoin, etc. (
  • This test looks for a specific auto-antibody called anti-CCP, which is present in an estimated 60 to 80 percent of people with RA, according to the Arthritis Foundation . (
  • The anti-CCP test is similar to the rheumatoid factor antibody test, which features later on in this article. (
  • This test looks for high levels of antinuclear antibodies, which are compounds that can attack a cell's nucleus, destroying the cell. (
  • And if the ANA test is positive, your blood can be tested for the presence of particular antinuclear antibodies, some of which are specific to certain diseases. (
  • The presence of any antinuclear antibodies is a positive test result. (
  • Global Demand for Antinuclear Antibody Test market was valued at approximately USD 951.2 million in 2019, and is expected to generate revenue of around USD 2038.2 million by end of 2026, growing at a CAGR of around 11.5 % between 2020 and 2026. (
  • The anti-nuclear antibody test detects autoimmune diseases, such as lupus or rheumatoid arthritis. (
  • A blood test determines the presence of antibodies to polynucleotides, histones and DNA. (
  • The presence of antibodies is also tested for by doing the ANA test. (
  • An Antinuclear antibodies test is used to determine if you may have an autoimmune disorder. (
  • If you present yourself with symptoms like Fever, red butterfly shaped rashes that are seen in Lupus, fatigues, joint pain accompanied with swelling and muscle pain, the doctor will order an Antinuclear Antibodies test to diagnose the exact autoimmune disorder that you may be suffering from. (
  • Presence of antinuclear antibodies in the test means that the person is most likely suffering from an autoimmune condition. (
  • The antinuclear antibody (ANA) test is used as a primary test to help evaluate a person for autoimmune disorders that affect many tissues and organs throughout the body and is most often used as one of the tests to help diagnose systemic lupus erythematosus. (
  • Do not test antinuclear antibody subserologies without a positive antinuclear antibody test result and clinical suspicion of immune-mediated disease. (
  • In certain patients antinuclear antibodies are "benign" while in systemic lupus with nephritis they are involved in production of nephritis by deposition as immune complexes with nuclear antigens and complement. (
  • We have found that, in the former, complement-fixing activity of such antibodies is low, even though present in large amounts, while in lupus nephritis complement-fixing activity is high. (
  • Variability in Antinuclear Antibody Testing to Assess Patient Eligibility for Clinical Trials of Novel Treatments for Systemic Lupus Erythematosus. (
  • In the development of novel therapies for systemic lupus erythematosus, antinuclear antibody (ANA) positivity represents a criterion for trial eligibility. (
  • We evaluated 5 commercially available HEp-2 antinuclear antibody (ANA) indirect fluorescent antibody (IFA) assays using patient serum samples from 45 patients with rheumatoid arthritis, 50 with systemic lupus erythematosus (SLE), 35 with scleroderma, 20 with Sjögren syndrome, 10 with polymyositis, and 100 healthy control subjects. (
  • The antibody in this systemic lupus erythematosus serum reacts with proliferating cell nuclear antigen (PCNA), identified as an auxiliary protein of DNA polymerase delta that is involved in DNA synthesis. (
  • By disease, global antinuclear antibody tests market is segmented into rheumatoid arthritis, systemic lupus erythematosus, Sjögren's syndrome, scleroderma and other diseases. (
  • Systemic lupus erythematosus and nephritis: severe relapse with disappearance of antinuclear antibodies. (
  • antiâ€"SS-A antibody - an antinuclear antibody that occurs in Sjцgren syndrome and systemic lupus erythematosus. (
  • Labs at diagnosis of antagonist-induced lupus-like syndromes Serological marker Result Anti-nuclear antibody 1:640 (previously 1:160 @ [RA.sup. (
  • Anti-nuclear antibodies (ANA) are possible signs of autoimmune diseases, such as lupus, scleroderma, Sjögren's syndrome, juvenile arthritis, or polymyositis and dermatomyositis. (
  • These same antibodies appear positive in women with lupus, rheumatoid arthritis, Crohn's disease and other autoimmune diseases. (
  • In ELISA, the antigen-antibody complexes are detected by another antibody which binds to human antibodies, but which carries an enzyme as a marker. (
  • Results show that detection of antinuclear antibodies by this sensor agrees quantitatively with the clinically accepted enzyme-linked immunosorbent assay (ELISA) method. (
  • We wanted to keep ANA IFA as our principle screening method, possibly reserving multiplex or ELISA methods to determine specific antibodies-a decision with which our physicians concurred. (
  • It certainly detects more antibodies than any other method, but ELISA and multiplex techniques specifically identify which antibody a patient has as opposed to providing a pattern in the IFA procedure. (
  • Global Anti-nuclear Antibody (ANA) Testing Market has been segmented on the basis of type which comprise immunofluorescence (Primary or direct and Secondary or indirect), enzymelinked, immunosorbent assay (ELISA) (Direct ELISA, Indirect ELISA, Sandwich ELISA, Competition/Inhibition ELISA), and others. (
  • The global antinuclear antibody tests market is segmented by technique type into enzyme-linked immunosorbent assay (ELISA), immunofluorescence assay and multiplex assay. (
  • Enzyme-Linked Immunosorbent Assay (ELISA) dominates the global antinuclear antibody tests market. (
  • Both these tests may be used by some laboratories together: the Immunoassay method (ELISA) screens for the presence of antinuclear antibodies, and IFA method subsequently confirms the positive or equivocal Immunoassay results. (
  • In normal individuals, the immune system produces antibodies to foreign proteins ( antigens ) but not to human proteins ( autoantigens ). (
  • Each of these antibody subtypes binds to different proteins or protein complexes within the nucleus. (
  • These cells coordinate an immune response upon the detection of foreign proteins ( antigens ), producing antibodies that bind to these antigens. (
  • [7] [14] The ENAs consist of ribonucleoproteins and non- histone proteins, named by either the name of the donor who provided the prototype serum (Sm, Ro, La, Jo), or the name of the disease setting in which the antibodies were found (SS-A, SS-B, Scl-70). (
  • Antibodies are proteins that your immune system makes to fight foreign substances like viruses and bacteria. (
  • Antibodies are proteins made by your immune system . (
  • Antibodies that attack healthy proteins within the nucleus - the control center of your cells - are called antinuclear antibodies (ANA). (
  • Antinuclear antibodies are antibodies made against antigens (proteins) in the nucleus of healthy cells. (
  • Antibody to deoxyribonucleic acid, ribonucleic acid, histone, or nonhistone proteins found in the serum of individuals with certain autoimmune diseases. (
  • In humans who are normal, antibodies to foreign proteins are produced, but in some abnormal cases, the immune system begins producing antibodies to human proteins known as autoantigens. (
  • When that happens, your body can make proteins called antibodies that attack your own cells. (
  • Antinuclear antibodies attack normal proteins in the center structure (nucleus) of your body's cells. (
  • The immune system makes an abundance of proteins called antibodies. (
  • When an antibody recognizes the foreign proteins of an infectious organism, it recruits other proteins and cells to fight off the infection. (
  • Sometimes these antibodies make a mistake, identifying normal, naturally-occurring proteins in our bodies as being "foreign" and dangerous. (
  • The antibodies that target "normal" proteins within the nucleus of a cell are called antinuclear antibodies (ANA). (
  • Sometimes, antibodies target normal proteins in our body by mistake. (
  • Antibodies are basically proteins produced by your immune system to fight foreign bodies, however in an autoimmune disorder, the body fights your own healthy cells by attacking the nucleus of these cells - hence the term anti-nuclear. (
  • They are found in many disorders including autoimmunity , cancer and infection , with different prevalences of antibodies depending on the condition. (
  • Serum antinuclear antibodies (ANA) are the most used autoimmunity markers in clinical practice at any level. (
  • Now, my question is, does testing "positive abnormal" on Antinuclear Direct imply a particular type of autoimmunity? (
  • In some cases, antibodies to human antigens are produced. (
  • Sometimes, however, this process malfunctions and antibodies are produced against human antigens, which may lead to autoimmune disease. (
  • Extractable nuclear antigens (ENA) are a group of autoantigens that were originally identified as antibody targets in people with autoimmune disorders. (
  • Antibodies are large protein molecules formed by the immune system to tag certain specific molecules (called 'antigens') on foreign or non-self substances, so that immune cells can latch on to them and destroy them. (
  • Biomolecular binding of antinuclear antibodies (ANA) with extractable nuclear antigens (ENA)-functionalized gold nanoparticles results in a change of surface plasmon absorption. (
  • Antinuclear antibodies are immunoglobulins or antibodies that bind to one or more antigens expressed within the nucleus of human cells. (
  • The second chapter describes each of the relevant antigens and the significance of the finding of antibody to each. (
  • Any of various antibodies that react with nuclear antigens such as nucleic acids and histones and are often present in the blood of people with systemic autoimmune rheumatic diseases. (
  • Any of a number of circulating antibodies directed against various antigens in the nucleus, including histone, double- and single-stranded DNA, and ribonucleoprotein. (
  • SmRNP is the best antigen for detection of antibodies to either Sm or RNP but cannot discriminate between the antibody specificities. (
  • The antibodies are directed against salivary duct antigen, increase in [beta]-2 microglobulin in saliva and synovial fluid, general immune abnormalities including hypergammaglobulinemia, rheumatoid factor, antinuclear antibody , anti-DNA (Deoxyribonucleic acid) antibody and increased Erythrocyte Sedimentation Rate levels have been observed (4). (
  • antibody - An immunoglobulin molecule produced by B lymphoid cells with a specific amino acid sequence evoked in humans or other animals by an antigen (immunogen). (
  • antinuclear - Having an affinity for or reacting with the cell nucleus. (
  • The report contains the intensity of reaction (corresponding to the ANA quantity) as well as the staining pattern, which reflects the way the antibody binds to the nucleus. (
  • It's called "antinuclear" because it targets the nucleus (center) of the cells. (
  • An unusual antibody that is directed against structures within the nucleus of the cell. (
  • The antibodies in the serum of the blood are exposed in the laboratory to cells and then one determines whether or not antibodies are present that react with various parts of the nucleus of the cell. (
  • ANA are antibodies produced by the immune system of the body against protein markers found in the nucleus of its own cells. (
  • In contrast, antinuclear antibodies often attack your body's own tissues - specifically targeting each cell's nucleus. (
  • Antinuclear Antibodies are abnormal antibodies made by the body. (
  • My aunt tested "positive abnormal" on Antinuclear Antibodies Direct. (
  • Homogeneous immunofluorescence staining pattern of double stranded DNA antibodies on HEp-20-10 cells. (
  • Speckled Immunofluorescence staining pattern of anti-nuclear antibodies on HEp-20-10 cells. (
  • Detection of antinuclear antibodies: Comparative evaluation of enzyme immunoassay and indirect immunofluorescence methods. (
  • The results of antinuclear antibody testing were negative (indirect immunofluorescence, screening dilution 1:40). (
  • The results of antinuclear antibody tests using the indirect immunofluorescence technique may be reported as a description of the pattern and the intensity of fluorescence obtained at a certain dilution. (
  • Immunosorbent assay linked to enzymes and indirect immunofluorescence are the most commonly used types of tests for the detection of antibodies. (
  • Different antinuclear antibodies generate distinctive patterns on immunofluorescence staining tests. (
  • Devreese, "Automated indirect immunofluorescence microscopy enables the implementation of a quantitative internal quality control system for anti-nuclear antibody (ANA) analysis," Clinical Chemistry and Laboratory Medicine, vol. (
  • This sensing platform has the following advantages: label-free and real-time detection capability, simple to construct and use, highly sensitive, and does not require a secondary antibody. (
  • 2012) Antinuclear Antibody Detection by Automated Multiplex Immunoassay in Untreated Patients at the Time of Diagnosis. (
  • Furthermore, early detection of these antibodies may lead to improved management of asbestos related diseases as well. (
  • Investigation of antinuclear antibodies (ANA) and techniques for detection. (
  • Antinuclear antibodies (ANA) detection is often needed to aid the diagnosis in several autoimmune disorders. (
  • To determine antinuclear antibody frequency and any possible correlation with clinical and laboratory data in sarcoidosis patients. (
  • Multiplex methods may identify more pa-tients with multiple antibodies and may provide improved clinical disease association for specific antibod-ies. (
  • This study used 5 commercially available IFAs to assess the ANA status of 181 patients enrolled in a phase II clinical trial for an anti-interleukin-6 antibody. (
  • Clinical features and evolution of antinuclear antibody positive individuals in a rheumatology outpatient clinic. (
  • Antinuclear Antibodies: Contemporary Techniques and Clinical Application to Connective Tissue Diseases. (
  • This succinct and up-to-date monograph defines both the clinical relevance and technical vagaries of tests for antinuclear antibodies. (
  • These patterns have clinical relevance and reflect which nuclear constituents (autoantigens) are generative specific antibody responses. (
  • Single-stranded DNA antibody: 70% of patients with SLE but also in other autoimmune rheumatic and inflammatory conditions, and is therefore of limited clinical value. (
  • A 15-year-old girl with a past medical history of asthma presented to our pediatric rheumatology outpatient clinic for evaluation of knee pain, which was associated with a positive antinuclear antibody (ANA). (
  • Association between antinuclear antibody titers and connective tissue diseases in a Rheumatology Department. (
  • If antinuclear antibodies are in the serum, they bind to the nuclei of cells on the slide. (
  • All patients were subjected to serum estimations of antinuclear antibodies, anticentromere antibodies and anti-Scl70 antibodies. (
  • According to the presence of antinuclear antibodies (ANA) at baseline and during the first 6 months of treatment, patients were sorted in different groups. (
  • Antibodies to porphyromonas gingivalis are associated with anticitrullinated protein antibodies in patients with rheumatoid arthritis and their relatives. (
  • Rheumatoid arthritis currently occupies the largest market share of global antibody tests market due to rising prevalence of rheumatoid arthritis. (
  • Development of connective tissue disease in patients presenting with Raynaud's phenomenon: A six year follow-up with emphasis on the predictive value of antinuclear antibodies as detected by immunoblotting. (
  • Antibody - A special protein built by the immune system as a defense against foreign material entering the body. (
  • By Western blot, this antibody detects a protein at ~270 kDa representing N-CoR from HeLa cell extracts and mouse RAW 264.7/primary macrophages. (
  • Recommended labs include a complete blood count and an additional work-up for anemia, complete metabolic panel, urinalysis, erythrocyte sedimentation rate, C-reactive protein, antinuclear antibody , complement C3 and C4, and antiphospholipid and anticardiolipin antibodies. (
  • As such, the serology is normal: There is no elevation in erythrocyte sedimentation rate or C-reactive protein, and antinuclear antibody and rheumatoid factor testing will be negative. (
  • Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies. (
  • Relative complement-fixing activity of antinuclear antibodies was determined by comparing titers in the complement fluorescent technique with titers by conventional antinuclear antibodies. (
  • Antinuclear antibodies are measured in titers. (
  • ANA are antibodies produced by the immune system that bind to the body's own tissues. (
  • Antinuclear antibodies are substances produced by the immune system that attack the body's own tissues. (
  • In autoimmune diseases, the body makes antibodies that work against its own cells or tissues. (
  • Sometimes antibodies mistakenly target your healthy cells and tissues. (
  • Antinuclear antibodies often attack on own tissues. (
  • These antibodies form in the blood when the body mistakes its own cells and tissues as foreign material and starts fighting against itself. (
  • Nuclear Pore Complex antibody LS-C194935 is an unconjugated mouse monoclonal antibody to human Nuclear Pore Complex. (
  • What Causes Elevated Anti-Nuclear Antibody ANA Level? (
  • We begin by identifying the disease conditions which have "elevated anti-nuclear antibody ANA level" as a symptom. (
  • Your search returned 139 nuclear transcription factor Y subunit beta Antibodies across 25 suppliers. (
  • Your search returned 494 nuclear receptor subfamily 2 group F member 2 Antibodies across 33 suppliers. (
  • Increasing technological advancements, adoption of new techniques and increasing prevalence of various autoimmune diseases are driving the growth for global anti-nuclear antibody (ANA) testing market. (
  • The Global Anti-Nuclear Antibody (ANA) Testing Market is growing at the CAGR of ~9.6% during the forecast period and expected to reach US$ 1300.6 million by 2023. (
  • Considering the global scenario of the market, North America is largest market for Anti-nuclear Antibody (ANA) Testing, in North America region US hold largest market share while European market is second largest, and especially the growth rate is higher in west European countries. (
  • The antibody was raised using a nuclear pore complex mixture. (
  • Small volumes of anti-nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. (
  • However, it is unknown whether these elevations are seen in anti-nuclear antibody-positive (ANA + ) individuals who lack sufficient criteria for a SARD diagnosis. (
  • A Japanese court has ruled against restarting a nuclear power plant in a rare victory for antinuclear activists after the Fukushima disaster, and dealing a blow to government efforts to end a nationwide nuclear freeze. (
  • Six of 11 mice developed IgM anti-single-stranded DNA antibodies shortly after receiving pristane and 4 developed IgM anti-histone antibodies, but anti-double-stranded DNA antibodies were absent. (
  • These antibodies can be against pure double stranded DNA (ds DNA), single stranded DNA (ss DNA), or smaller molecules called polynucleotides and histones that make up the single strands. (
  • Anti-double stranded DNA antibody (anti-dsDNA): associated with SLE. (
  • Rheumatic diseases (diseases that affect connective tissue , including the joints, bone, and muscle) are also associated with these antibodies. (
  • In these kinds of connective-tissue diseases, the immune-system antibodies that normally protect you from disease turn against your body's own cells. (
  • Viral or bacterial infections, lung diseases (such as pulmonary hypertension), ulcerative colitis, cancers (of the skin, breast, lung and kidney) and even skin conditions like psoriasis can cause an increase in the number of antibodies produced. (
  • Diagnosis is further complicated by the considerable overlap in symptoms of many connective-tissue diseases as well as the presence of various antibodies. (
  • Antinuclear antibodies are the markers used for connective tissue diseases. (
  • RNP antibodies are found with a variety of rheumatoid diseases. (
  • Antinuclear antibody (ANA) testing for many years has been the primary laboratory screening approach for diagnosing connective tissue autoimmune diseases. (
  • In the literature, there are case reports suggesting that Borrelia burgdorferi infection may induce autoimmune diseases dependent on antinuclear antibodies (ANA). (
  • Prevalence of antinuclear antibodies in 3 groups of healthy individuals: blood donors, hospital personnel, and relatives of patients with autoimmune diseases. (
  • Vitamin D deficiency is associated with cancer and autoimmune diseases, but little is known about the association between vitamin D and antinuclear antibodies (ANA), a biomarker of immune dysfunction in healthy populations. (
  • Antibodies to p62 complex are involved in 1 or more autoimmune diseases. (
  • Antinuclear antibodies are found in many autoimmune diseases. (
  • In women with autoimmune diseases these antibodies cause inflammation in joints and organs. (
  • In women with no autoimmune diseases but a positive antibody, the antibody causes inflammation around the embryo at the time of implantation or in the placenta after implantation. (
  • Immune system makes antibodies to fight with various infections. (
  • Your immune system normally makes antibodies to help you fight infection. (
  • Laboratory tests include relatively nonspecific antinuclear antibody (ANA) testing and/or tests for individual antibodies that are more disease specific. (
  • Additional testing, for example with specific antibody tests, should be considered if clinically warranted ( Table 2 ). (
  • These tests can find out which antibodies are in the blood in higher amounts than normal. (
  • Evidence-based guidelines for the use of immunologic tests: antinuclear antibody testing. (
  • An antinuclear antibody tests (ANA) are used to evaluate quantity and pattern of antibodies in the blood. (
  • The global antinuclear antibody tests market is segmented by product type into reagent & assays kits, systems and software & services. (
  • Reagents and assay kits occupy the largest share of global antinuclear antibody tests market. (
  • As of the current market scenario, North America dominated the global antinuclear antibody tests market followed by the Europe. (
  • Asia Pacific is the fastest growing regional market for antinuclear antibody tests. (
  • in these cats, anemia and thrombocytopenia resolved and Coombs' and antinuclear antibody tests became negative within 2 weeks. (
  • Detect antibodies associated with SLE and mixed connective tissue disease. (
  • The first 30 pages provide the most organized and clear synopsis I have seen of the various antinuclear antibodies and their relations to individual connective tissue syndromes. (
  • The Antinuclear Antibodies (ANA) Comprehensive Profile detects antibodies associated with SLE and mixed connective tissue disease. (
  • ENAs and antibodies to them are found in various combinations in individuals with combinations of overlapping rheumatologic symptoms. (
  • The two patients who had anticentromere and anti-Scl-70 antibodies had also Sjögren's syndrome and scleroderma diagnosis, respectively. (
  • Frequent positivity of IgM antibodies suggests that persons in the early phase of infection prevailed in the group. (
  • This defence mechanism produces antibodies (large glycoproteins ) in response to an immune stimulus. (
  • When a person has an autoimmune disease, the immune system produces antibodies that attach to the body's own cells as though they were foreign substances, often causing them to be damaged or destroyed. (
  • When the immune system identifies a cell as foreign or 'non-self', it produces antibodies against these cells to destroy them. (
  • Antinuclear antibodies were determined with indirect immunofluorescent method and 1/100 titration was accepted as positive. (
  • A 15-year-old girl presented with knee pain, associated with a positive antinuclear antibody (ANA). (
  • Circulating antibody to C dificile toxin was demonstrated in that patient, whereas 11 other patients with PMC-positive fecal toxin, but without arthritis, did not have circulating antibody to C dificile toxin (3). (
  • We describe a patient who had nitrofurantoin-induced panniculitis with associated serologic abnormalities of positive antinuclear antibody (ANA) and leukopenia. (
  • Whether the inflammatory arthritis in two of them (Case 10 and 15, Table 2) along with severe drug reaction and the positive antinuclear antibody status in all three has placed them at risk for multiple drug allergy remains to be seen. (
  • Pressure points are located within the first toric iol, which is a type of extracapsular extension and/or positive surgical margins at the base of the arterioles and stimulates the formation of antinuclear antibody prednisone clots to decrease possible neonatal abstinence scoring system. (
  • 15% of normal people have positive antinuclear antibodies without any disease. (
  • Going through my lab works, I now realize that I tested positive on "Antinuclear Direct" a year earlier on my nephrologist's blood order. (
  • A fiberoptic evanescent-wave sensor has been developed for the measurement of antinuclear antibodies in sera from patients and healthy individuals. (
  • We observed that many of these patients developed antinuclear antibodies (ANA) and antidouble-stranded DNA (anti-dsDNA) antibodies while on treatment prompting us to investigate whether their development is associated with anti-TNF treatment failure. (
  • Anti-dsDNA antibodies developed in 2%, 27%, 33% and 83% of patients from the same respective groups. (
  • Significantly, the antibodies developed before treatment had failed with all three agents and their development was not related to the total time that patients had been on anti-TNF therapy. (
  • We found an antinuclear antibody highly restricted to nuclei of neurons in two patients with subacute sensory neuronopathy complicating oat cell carcinoma of the lung. (
  • To delineate if immune mechanisms might also be participating in JME, we screened the sera of 19 JME and 21 healthy control patients for anti-neuronal antibodies using immunohistochemistry and Western blot techniques. (
  • JME patients' sera showed relatively but not significantly increased antinuclear/nucleolar antibody incidence and multiple Western blot bands as compared to healthy controls, suggesting humoral immune mechanisms might be participating in JME pathogenesis. (
  • Parietal cell antibodies may also be found in elderly patients without autoimmune disease. (
  • An unexplained high antinuclear antibodies titre in pleural or pericardial effusion warrants search for malignancy. (
  • The cytoplasm is nonreactive with the antibody and the nucleolar domains contain a few small speckles. (
  • Nucleolar: the antibody is directed to the polynucleotides. (
  • We report herein a case of a 16-year-old male who developed antinuclear antibody (ANA)-negative SLE and hypothyroidism after treatment with interferon-alpha for chronic hepatitis. (