Antibodies, Antinuclear: Autoantibodies directed against various nuclear antigens including DNA, RNA, histones, acidic nuclear proteins, or complexes of these molecular elements. Antinuclear antibodies are found in systemic autoimmune diseases including systemic lupus erythematosus, Sjogren's syndrome, scleroderma, polymyositis, and mixed connective tissue disease.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Antibody Specificity: The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.Antibodies, Viral: Immunoglobulins produced in response to VIRAL ANTIGENS.Autoantibodies: Antibodies that react with self-antigens (AUTOANTIGENS) of the organism that produced them.Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Antibodies, Bacterial: Immunoglobulins produced in a response to BACTERIAL ANTIGENS.Lupus Erythematosus, Systemic: A chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow.Fluorescent Antibody Technique: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.Antibody Formation: The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.Antibodies, Neutralizing: Antibodies that reduce or abolish some biological activity of a soluble antigen or infectious agent, usually a virus.Connective Tissue Diseases: A heterogeneous group of disorders, some hereditary, others acquired, characterized by abnormal structure or function of one or more of the elements of connective tissue, i.e., collagen, elastin, or the mucopolysaccharides.Rheumatoid Factor: Antibodies found in adult RHEUMATOID ARTHRITIS patients that are directed against GAMMA-CHAIN IMMUNOGLOBULINS.Fluorescent Antibody Technique, Indirect: A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)Antibodies, Anti-Idiotypic: Antibodies which react with the individual structural determinants (idiotopes) on the variable region of other antibodies.Autoimmune Diseases: Disorders that are characterized by the production of antibodies that react with host tissues or immune effector cells that are autoreactive to endogenous peptides.Antibody Affinity: A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes.Immunoglobulin M: A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.Immunoglobulin G: The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.Binding Sites, Antibody: Local surface sites on antibodies which react with antigen determinant sites on antigens (EPITOPES.) They are formed from parts of the variable regions of FAB FRAGMENTS.Scleroderma, Systemic: A chronic multi-system disorder of CONNECTIVE TISSUE. It is characterized by SCLEROSIS in the SKIN, the LUNGS, the HEART, the GASTROINTESTINAL TRACT, the KIDNEYS, and the MUSCULOSKELETAL SYSTEM. Other important features include diseased small BLOOD VESSELS and AUTOANTIBODIES. The disorder is named for its most prominent feature (hard skin), and classified into subsets by the extent of skin thickening: LIMITED SCLERODERMA and DIFFUSE SCLERODERMA.HIV Antibodies: Antibodies reactive with HIV ANTIGENS.Cross Reactions: Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.Epitopes: Sites on an antigen that interact with specific antibodies.Antibodies, Neoplasm: Immunoglobulins induced by antigens specific for tumors other than the normally occurring HISTOCOMPATIBILITY ANTIGENS.Antibodies, Protozoan: Immunoglobulins produced in a response to PROTOZOAN ANTIGENS.Rheumatic Diseases: Disorders of connective tissue, especially the joints and related structures, characterized by inflammation, degeneration, or metabolic derangement.Sjogren's Syndrome: Chronic inflammatory and autoimmune disease in which the salivary and lacrimal glands undergo progressive destruction by lymphocytes and plasma cells resulting in decreased production of saliva and tears. The primary form, often called sicca syndrome, involves both KERATOCONJUNCTIVITIS SICCA and XEROSTOMIA. The secondary form includes, in addition, the presence of a connective tissue disease, usually rheumatoid arthritis.Hepatitis, Autoimmune: A chronic self-perpetuating hepatocellular INFLAMMATION of unknown cause, usually with HYPERGAMMAGLOBULINEMIA and serum AUTOANTIBODIES.Enzyme-Linked Immunosorbent Assay: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.Autoantigens: Endogenous tissue constituents that have the ability to interact with AUTOANTIBODIES and cause an immune response.Raynaud Disease: An idiopathic vascular disorder characterized by bilateral Raynaud phenomenon, the abrupt onset of digital paleness or CYANOSIS in response to cold exposure or stress.Antigen-Antibody Complex: The complex formed by the binding of antigen and antibody molecules. The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES.Arthritis, Juvenile: Arthritis of children, with onset before 16 years of age. The terms juvenile rheumatoid arthritis (JRA) and juvenile idiopathic arthritis (JIA) refer to classification systems for chronic arthritis in children. Only one subtype of juvenile arthritis (polyarticular-onset, rheumatoid factor-positive) clinically resembles adult rheumatoid arthritis and is considered its childhood equivalent.Iritis: Inflammation of the iris characterized by circumcorneal injection, aqueous flare, keratotic precipitates, and constricted and sluggish pupil along with discoloration of the iris.Antibodies, Antiphospholipid: Autoantibodies directed against phospholipids. These antibodies are characteristically found in patients with systemic lupus erythematosus (LUPUS ERYTHEMATOSUS, SYSTEMIC;), ANTIPHOSPHOLIPID SYNDROME; related autoimmune diseases, some non-autoimmune diseases, and also in healthy individuals.Mixed Connective Tissue Disease: A syndrome with overlapping clinical features of systemic lupus erythematosus, scleroderma, polymyositis, and Raynaud's phenomenon. The disease is differentially characterized by high serum titers of antibodies to ribonuclease-sensitive extractable (saline soluble) nuclear antigen and a "speckled" epidermal nuclear staining pattern on direct immunofluorescence.Antibodies, Antineutrophil Cytoplasmic: Autoantibodies directed against cytoplasmic constituents of POLYMORPHONUCLEAR LEUKOCYTES and/or MONOCYTES. They are used as specific markers for GRANULOMATOSIS WITH POLYANGIITIS and other diseases, though their pathophysiological role is not clear. ANCA are routinely detected by indirect immunofluorescence with three different patterns: c-ANCA (cytoplasmic), p-ANCA (perinuclear), and atypical ANCA.Immunoglobulin A: Represents 15-20% of the human serum immunoglobulins, mostly as the 4-chain polymer in humans or dimer in other mammals. Secretory IgA (IMMUNOGLOBULIN A, SECRETORY) is the main immunoglobulin in secretions.Antibodies, Fungal: Immunoglobulins produced in a response to FUNGAL ANTIGENS.Mice, Inbred BALB CScleroderma, Localized: A term used to describe a variety of localized asymmetrical SKIN thickening that is similar to those of SYSTEMIC SCLERODERMA but without the disease features in the multiple internal organs and BLOOD VESSELS. Lesions may be characterized as patches or plaques (morphea), bands (linear), or nodules.Liver Cirrhosis, Biliary: FIBROSIS of the hepatic parenchyma due to obstruction of BILE flow (CHOLESTASIS) in the intrahepatic or extrahepatic bile ducts (BILE DUCTS, INTRAHEPATIC; BILE DUCTS, EXTRAHEPATIC). Primary biliary cirrhosis involves the destruction of small intra-hepatic bile ducts and bile secretion. Secondary biliary cirrhosis is produced by prolonged obstruction of large intrahepatic or extrahepatic bile ducts from a variety of causes.Lupus Nephritis: Glomerulonephritis associated with autoimmune disease SYSTEMIC LUPUS ERYTHEMATOSUS. Lupus nephritis is histologically classified into 6 classes: class I - normal glomeruli, class II - pure mesangial alterations, class III - focal segmental glomerulonephritis, class IV - diffuse glomerulonephritis, class V - diffuse membranous glomerulonephritis, and class VI - advanced sclerosing glomerulonephritis (The World Health Organization classification 1982).Neutralization Tests: The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).Antigen-Antibody Reactions: The processes triggered by interactions of ANTIBODIES with their ANTIGENS.Antibodies, Bispecific: Antibodies, often monoclonal, in which the two antigen-binding sites are specific for separate ANTIGENIC DETERMINANTS. They are artificial antibodies produced by chemical crosslinking, fusion of HYBRIDOMA cells, or by molecular genetic techniques. They function as the main mediators of targeted cellular cytotoxicity and have been shown to be efficient in the targeting of drugs, toxins, radiolabeled haptens, and effector cells to diseased tissue, primarily tumors.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Precipitins: Antibodies which elicit IMMUNOPRECIPITATION when combined with antigen.Immunoglobulins: Multi-subunit proteins which function in IMMUNITY. They are produced by B LYMPHOCYTES from the IMMUNOGLOBULIN GENES. They are comprised of two heavy (IMMUNOGLOBULIN HEAVY CHAINS) and two light chains (IMMUNOGLOBULIN LIGHT CHAINS) with additional ancillary polypeptide chains depending on their isoforms. The variety of isoforms include monomeric or polymeric forms, and transmembrane forms (B-CELL ANTIGEN RECEPTORS) or secreted forms (ANTIBODIES). They are divided by the amino acid sequence of their heavy chains into five classes (IMMUNOGLOBULIN A; IMMUNOGLOBULIN D; IMMUNOGLOBULIN E; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M) and various subclasses.Mice, Inbred NZBDNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Nephritis: Inflammation of any part of the KIDNEY.Single-Chain Antibodies: A form of antibodies consisting only of the variable regions of the heavy and light chains (FV FRAGMENTS), connected by a small linker peptide. They are less immunogenic than complete immunoglobulin and thus have potential therapeutic use.snRNP Core Proteins: The protein components that constitute the common core of small nuclear ribonucleoprotein particles. These proteins are commonly referred as Sm nuclear antigens due to their antigenic nature.Immunoenzyme Techniques: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.Antibodies, Blocking: Antibodies that inhibit the reaction between ANTIGEN and other antibodies or sensitized T-LYMPHOCYTES (e.g., antibodies of the IMMUNOGLOBULIN G class that compete with IGE antibodies for antigen, thereby blocking an allergic response). Blocking antibodies that bind tumors and prevent destruction of tumor cells by CYTOTOXIC T-LYMPHOCYTES have also been called enhancing antibodies. (Rosen et al., Dictionary of Immunology, 1989)Serositis: Inflammation of a serous membrane.Immunoassay: A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.Autoimmunity: Process whereby the immune system reacts against the body's own tissues. Autoimmunity may produce or be caused by AUTOIMMUNE DISEASES.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Rabbits: The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.Antigens: Substances that are recognized by the immune system and induce an immune reaction.Immunoglobulin Fab Fragments: Univalent antigen-binding fragments composed of one entire IMMUNOGLOBULIN LIGHT CHAIN and the amino terminal end of one of the IMMUNOGLOBULIN HEAVY CHAINS from the hinge region, linked to each other by disulfide bonds. Fab contains the IMMUNOGLOBULIN VARIABLE REGIONS, which are part of the antigen-binding site, and the first IMMUNOGLOBULIN CONSTANT REGIONS. This fragment can be obtained by digestion of immunoglobulins with the proteolytic enzyme PAPAIN.Myositis: Inflammation of a muscle or muscle tissue.Antigens, Nuclear: Immunologically detectable substances found in the CELL NUCLEUS.Antibodies, Anticardiolipin: Antiphospholipid antibodies found in association with systemic lupus erythematosus (LUPUS ERYTHEMATOSUS, SYSTEMIC;), ANTIPHOSPHOLIPID SYNDROME; and in a variety of other diseases as well as in healthy individuals. The antibodies are detected by solid-phase IMMUNOASSAY employing the purified phospholipid antigen CARDIOLIPIN.Antibodies, Heterophile: Antibodies elicited in a different species from which the antigen originated. These antibodies are directed against a wide variety of interspecies-specific antigens, the best known of which are Forssman, Hanganutziu-Deicher (H-D), and Paul-Bunnell (P-B). Incidence of antibodies to these antigens--i.e., the phenomenon of heterophile antibody response--is useful in the serodiagnosis, pathogenesis, and prognosis of infection and latent infectious states as well as in cancer classification.Serologic Tests: Diagnostic procedures involving immunoglobulin reactions.Antibodies, Catalytic: Antibodies that can catalyze a wide variety of chemical reactions. They are characterized by high substrate specificity and share many mechanistic features with enzymes.Immunodiffusion: Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.Sensitivity and Specificity: Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)Lupus Erythematosus, Cutaneous: A form of lupus erythematosus in which the skin may be the only organ involved or in which skin involvement precedes the spread into other body systems. It has been classified into three forms - acute (= LUPUS ERYTHEMATOSUS, SYSTEMIC with skin lesions), subacute, and chronic (= LUPUS ERYTHEMATOSUS, DISCOID).B-Lymphocytes: Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.Arthritis, Rheumatoid: A chronic systemic disease, primarily of the joints, marked by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Etiology is unknown, but autoimmune mechanisms have been implicated.Complement System Proteins: Serum glycoproteins participating in the host defense mechanism of COMPLEMENT ACTIVATION that creates the COMPLEMENT MEMBRANE ATTACK COMPLEX. Included are glycoproteins in the various pathways of complement activation (CLASSICAL COMPLEMENT PATHWAY; ALTERNATIVE COMPLEMENT PATHWAY; and LECTIN COMPLEMENT PATHWAY).Immunoblotting: Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.Antibodies, Monoclonal, Humanized: Antibodies from non-human species whose protein sequences have been modified to make them nearly identical with human antibodies. If the constant region and part of the variable region are replaced, they are called humanized. If only the constant region is modified they are called chimeric. INN names for humanized antibodies end in -zumab.Lupus Vulgaris: A form of cutaneous tuberculosis. It is seen predominantly in women and typically involves the NASAL MUCOSA; BUCCAL MUCOSA; and conjunctival mucosa.Crithidia: A genus of parasitic protozoans found in the digestive tract of invertebrates, especially insects. Organisms of this genus have an amastigote and choanomastigote stage in their life cycle.Hybridomas: Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell.Immune Sera: Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.Lupus Erythematosus, Discoid: A chronic form of cutaneous lupus erythematosus (LUPUS ERYTHEMATOSUS, CUTANEOUS) in which the skin lesions mimic those of the systemic form but in which systemic signs are rare. It is characterized by the presence of discoid skin plaques showing varying degrees of edema, erythema, scaliness, follicular plugging, and skin atrophy. Lesions are surrounded by an elevated erythematous border. The condition typically involves the face and scalp, but widespread dissemination may occur.Complement C4: A glycoprotein that is important in the activation of CLASSICAL COMPLEMENT PATHWAY. C4 is cleaved by the activated COMPLEMENT C1S into COMPLEMENT C4A and COMPLEMENT C4B.Epitope Mapping: Methods used for studying the interactions of antibodies with specific regions of protein antigens. Important applications of epitope mapping are found within the area of immunochemistry.Glomerulonephritis: Inflammation of the renal glomeruli (KIDNEY GLOMERULUS) that can be classified by the type of glomerular injuries including antibody deposition, complement activation, cellular proliferation, and glomerulosclerosis. These structural and functional abnormalities usually lead to HEMATURIA; PROTEINURIA; HYPERTENSION; and RENAL INSUFFICIENCY.Immunization: Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).RNA, Small Cytoplasmic: Small RNAs found in the cytoplasm usually complexed with proteins in scRNPs (RIBONUCLEOPROTEINS, SMALL CYTOPLASMIC).Cell Line: Established cell cultures that have the potential to propagate indefinitely.Mice, Inbred Strains: Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.False Positive Reactions: Positive test results in subjects who do not possess the attribute for which the test is conducted. The labeling of healthy persons as diseased when screening in the detection of disease. (Last, A Dictionary of Epidemiology, 2d ed)Time Factors: Elements of limited time intervals, contributing to particular results or situations.Ribonucleoproteins: Complexes of RNA-binding proteins with ribonucleic acids (RNA).Antigens, Bacterial: Substances elaborated by bacteria that have antigenic activity.Spleen: An encapsulated lymphatic organ through which venous blood filters.Dermatomyositis: A subacute or chronic inflammatory disease of muscle and skin, marked by proximal muscle weakness and a characteristic skin rash. The illness occurs with approximately equal frequency in children and adults. The skin lesions usually take the form of a purplish rash (or less often an exfoliative dermatitis) involving the nose, cheeks, forehead, upper trunk, and arms. The disease is associated with a complement mediated intramuscular microangiopathy, leading to loss of capillaries, muscle ischemia, muscle-fiber necrosis, and perifascicular atrophy. The childhood form of this disease tends to evolve into a systemic vasculitis. Dermatomyositis may occur in association with malignant neoplasms. (From Adams et al., Principles of Neurology, 6th ed, pp1405-6)Mice, Inbred C57BLBiological Markers: Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.Antigens, Surface: Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.Cardiolipins: Acidic phospholipids composed of two molecules of phosphatidic acid covalently linked to a molecule of glycerol. They occur primarily in mitochondrial inner membranes and in bacterial plasma membranes. They are the main antigenic components of the Wassermann-type antigen that is used in nontreponemal SYPHILIS SERODIAGNOSIS.Immunization, Passive: Transfer of immunity from immunized to non-immune host by administration of serum antibodies, or transplantation of lymphocytes (ADOPTIVE TRANSFER).Antiphospholipid Syndrome: The presence of antibodies directed against phospholipids (ANTIBODIES, ANTIPHOSPHOLIPID). The condition is associated with a variety of diseases, notably systemic lupus erythematosus and other connective tissue diseases, thrombopenia, and arterial or venous thromboses. In pregnancy it can cause abortion. Of the phospholipids, the cardiolipins show markedly elevated levels of anticardiolipin antibodies (ANTIBODIES, ANTICARDIOLIPIN). Present also are high levels of lupus anticoagulant (LUPUS COAGULATION INHIBITOR).Recombinant Proteins: Proteins prepared by recombinant DNA technology.T-Lymphocytes: Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.Vasculitis: Inflammation of any one of the blood vessels, including the ARTERIES; VEINS; and rest of the vasculature system in the body.gamma-Globulins: Serum globulins that migrate to the gamma region (most positively charged) upon ELECTROPHORESIS. At one time, gamma-globulins came to be used as a synonym for immunoglobulins since most immunoglobulins are gamma globulins and conversely most gamma globulins are immunoglobulins. But since some immunoglobulins exhibit an alpha or beta electrophoretic mobility, that usage is in decline.HLA-DR5 Antigen: A broad-specificity HLA-DR antigen that is associated with HLA-DRB1 CHAINS encoded by DRB1*11 and DRB1*12 alleles.Immunoglobulin Fragments: Partial immunoglobulin molecules resulting from selective cleavage by proteolytic enzymes or generated through PROTEIN ENGINEERING techniques.Molecular Weight: The sum of the weight of all the atoms in a molecule.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.Kidney Glomerulus: A cluster of convoluted capillaries beginning at each nephric tubule in the kidney and held together by connective tissue.Antigens, Viral: Substances elaborated by viruses that have antigenic activity.ArthritisRibonucleoproteins, Small Nuclear: Highly conserved nuclear RNA-protein complexes that function in RNA processing in the nucleus, including pre-mRNA splicing and pre-mRNA 3'-end processing in the nucleoplasm, and pre-rRNA processing in the nucleolus (see RIBONUCLEOPROTEINS, SMALL NUCLEOLAR).Salivary Glands: Glands that secrete SALIVA in the MOUTH. There are three pairs of salivary glands (PAROTID GLAND; SUBLINGUAL GLAND; SUBMANDIBULAR GLAND).Radioimmunoassay: Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Immune Complex Diseases: Group of diseases mediated by the deposition of large soluble complexes of antigen and antibody with resultant damage to tissue. Besides SERUM SICKNESS and the ARTHUS REACTION, evidence supports a pathogenic role for immune complexes in many other IMMUNE SYSTEM DISEASES including GLOMERULONEPHRITIS, systemic lupus erythematosus (LUPUS ERYTHEMATOSUS, SYSTEMIC) and POLYARTERITIS NODOSA.HLA Antigens: Antigens determined by leukocyte loci found on chromosome 6, the major histocompatibility loci in humans. They are polypeptides or glycoproteins found on most nucleated cells and platelets, determine tissue types for transplantation, and are associated with certain diseases.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Complement C3: A glycoprotein that is central in both the classical and the alternative pathway of COMPLEMENT ACTIVATION. C3 can be cleaved into COMPLEMENT C3A and COMPLEMENT C3B, spontaneously at low level or by C3 CONVERTASE at high level. The smaller fragment C3a is an ANAPHYLATOXIN and mediator of local inflammatory process. The larger fragment C3b binds with C3 convertase to form C5 convertase.Cell Nucleolus: Within most types of eukaryotic CELL NUCLEUS, a distinct region, not delimited by a membrane, in which some species of rRNA (RNA, RIBOSOMAL) are synthesized and assembled into ribonucleoprotein subunits of ribosomes. In the nucleolus rRNA is transcribed from a nucleolar organizer, i.e., a group of tandemly repeated chromosomal genes which encode rRNA and which are transcribed by RNA polymerase I. (Singleton & Sainsbury, Dictionary of Microbiology & Molecular Biology, 2d ed)Complement Fixation Tests: Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.Procainamide: A class Ia antiarrhythmic drug that is structurally-related to PROCAINE.Disease Models, Animal: Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.Hemagglutination Tests: Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed)Blood Sedimentation: Measurement of rate of settling of erythrocytes in anticoagulated blood.Hemagglutination Inhibition Tests: Serologic tests in which a known quantity of antigen is added to the serum prior to the addition of a red cell suspension. Reaction result is expressed as the smallest amount of antigen which causes complete inhibition of hemagglutination.Immunoglobulin Variable Region: That region of the immunoglobulin molecule that varies in its amino acid sequence and composition, and comprises the binding site for a specific antigen. It is located at the N-terminus of the Fab fragment of the immunoglobulin. It includes hypervariable regions (COMPLEMENTARITY DETERMINING REGIONS) and framework regions.Seroepidemiologic Studies: EPIDEMIOLOGIC STUDIES based on the detection through serological testing of characteristic change in the serum level of specific ANTIBODIES. Latent subclinical infections and carrier states can thus be detected in addition to clinically overt cases.Lymphocyte Activation: Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.Reagent Kits, Diagnostic: Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.Sialadenitis: INFLAMMATION of salivary tissue (SALIVARY GLANDS), usually due to INFECTION or injuries.Hypergammaglobulinemia: An excess of GAMMA-GLOBULINS in the serum due to chronic infections or PARAPROTEINEMIAS.Immunoglobulin Idiotypes: Unique genetically-controlled determinants present on ANTIBODIES whose specificity is limited to a single group of proteins (e.g., another antibody molecule or an individual myeloma protein). The idiotype appears to represent the antigenicity of the antigen-binding site of the antibody and to be genetically codetermined with it. The idiotypic determinants have been precisely located to the IMMUNOGLOBULIN VARIABLE REGION of both immunoglobin polypeptide chains.Immunologic Techniques: Techniques used to demonstrate or measure an immune response, and to identify or measure antigens using antibodies.Lymphocytes: White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.Toxoplasmosis: The acquired form of infection by Toxoplasma gondii in animals and man.Mice, Congenic: Mouse strains constructed to possess identical genotypes except for a difference at a single gene locus.Antigens, Neoplasm: Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Immunosorbent Techniques: Techniques for removal by adsorption and subsequent elution of a specific antibody or antigen using an immunosorbent containing the homologous antigen or antibody.Haptens: Small antigenic determinants capable of eliciting an immune response only when coupled to a carrier. Haptens bind to antibodies but by themselves cannot elicit an antibody response.Kidney: Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.Mice, Inbred MRL lpr: A mouse substrain that is genetically predisposed to the development of systemic lupus erythematosus-like syndrome, which has been found to be clinically similar to the human disease. It has been determined that this mouse strain carries a mutation in the fas gene. Also, the MRL/lpr is a useful model to study behavioral and cognitive deficits found in autoimmune diseases and the efficacy of immunosuppressive agents.Splenomegaly: Enlargement of the spleen.Antibody Diversity: The phenomenon of immense variability characteristic of ANTIBODIES. It enables the IMMUNE SYSTEM to react specifically against the essentially unlimited kinds of ANTIGENS it encounters. Antibody diversity is accounted for by three main theories: (1) the Germ Line Theory, which holds that each antibody-producing cell has genes coding for all possible antibody specificities, but expresses only the one stimulated by antigen; (2) the Somatic Mutation Theory, which holds that antibody-producing cells contain only a few genes, which produce antibody diversity by mutation; and (3) the Gene Rearrangement Theory, which holds that antibody diversity is generated by the rearrangement of IMMUNOGLOBULIN VARIABLE REGION gene segments during the differentiation of the ANTIBODY-PRODUCING CELLS.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Peptide Library: A collection of cloned peptides, or chemically synthesized peptides, frequently consisting of all possible combinations of amino acids making up an n-amino acid peptide.Deoxyribonucleases: Enzymes which catalyze the hydrolases of ester bonds within DNA. EC 3.1.-.Biopsy: Removal and pathologic examination of specimens in the form of small pieces of tissue from the living body.Hepatitis C Antibodies: Antibodies to the HEPATITIS C ANTIGENS including antibodies to envelope, core, and non-structural proteins.Nucleoproteins: Proteins conjugated with nucleic acids.Isoantibodies: Antibodies from an individual that react with ISOANTIGENS of another individual of the same species.Immunoglobulin Isotypes: The classes of immunoglobulins found in any species of animal. In man there are nine classes that migrate in five different groups in electrophoresis; they each consist of two light and two heavy protein chains, and each group has distinguishing structural and functional properties.Spondylitis, Ankylosing: A chronic inflammatory condition affecting the axial joints, such as the SACROILIAC JOINT and other intervertebral or costovertebral joints. It occurs predominantly in young males and is characterized by pain and stiffness of joints (ANKYLOSIS) with inflammation at tendon insertions.Flow Cytometry: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.Thyroid Gland: A highly vascularized endocrine gland consisting of two lobes joined by a thin band of tissue with one lobe on each side of the TRACHEA. It secretes THYROID HORMONES from the follicular cells and CALCITONIN from the parafollicular cells thereby regulating METABOLISM and CALCIUM level in blood, respectively.Histones: Small chromosomal proteins (approx 12-20 kD) possessing an open, unfolded structure and attached to the DNA in cell nuclei by ionic linkages. Classification into the various types (designated histone I, histone II, etc.) is based on the relative amounts of arginine and lysine in each.Antibodies, Monoclonal, Murine-Derived: Antibodies obtained from a single clone of cells grown in mice or rats.Retrospective Studies: Studies used to test etiologic hypotheses in which inferences about an exposure to putative causal factors are derived from data relating to characteristics of persons under study or to events or experiences in their past. The essential feature is that some of the persons under study have the disease or outcome of interest and their characteristics are compared with those of unaffected persons.Glycoproteins: Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.Vaccination: Administration of vaccines to stimulate the host's immune response. This includes any preparation intended for active immunological prophylaxis.Chronic Disease: Diseases which have one or more of the following characteristics: they are permanent, leave residual disability, are caused by nonreversible pathological alteration, require special training of the patient for rehabilitation, or may be expected to require a long period of supervision, observation, or care. (Dictionary of Health Services Management, 2d ed)Hepatitis B Antibodies: Antibodies to the HEPATITIS B ANTIGENS, including antibodies to the surface (Australia) and core of the Dane particle and those to the "e" antigens.Binding, Competitive: The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.Histocytochemistry: Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.Peptide Fragments: Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.Immunity, Maternally-Acquired: Resistance to a disease-causing agent induced by the introduction of maternal immunity into the fetus by transplacental transfer or into the neonate through colostrum and milk.Microscopy, Fluorescence: Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.Insulin Antibodies: Antibodies specific to INSULIN.Neutrophils: Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.Case-Control Studies: Studies which start with the identification of persons with a disease of interest and a control (comparison, referent) group without the disease. The relationship of an attribute to the disease is examined by comparing diseased and non-diseased persons with regard to the frequency or levels of the attribute in each group.Antirheumatic Agents: Drugs that are used to treat RHEUMATOID ARTHRITIS.Reproducibility of Results: The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.Centromere: The clear constricted portion of the chromosome at which the chromatids are joined and by which the chromosome is attached to the spindle during cell division.Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.Precipitin Tests: Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.DNA, Single-Stranded: A single chain of deoxyribonucleotides that occurs in some bacteria and viruses. It usually exists as a covalently closed circle.Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.Antigens, Protozoan: Any part or derivative of any protozoan that elicits immunity; malaria (Plasmodium) and trypanosome antigens are presently the most frequently encountered.Peptides: Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.Penicillamine: 3-Mercapto-D-valine. The most characteristic degradation product of the penicillin antibiotics. It is used as an antirheumatic and as a chelating agent in Wilson's disease.Antibody-Dependent Cell Cytotoxicity: The phenomenon of antibody-mediated target cell destruction by non-sensitized effector cells. The identity of the target cell varies, but it must possess surface IMMUNOGLOBULIN G whose Fc portion is intact. The effector cell is a "killer" cell possessing Fc receptors. It may be a lymphocyte lacking conventional B- or T-cell markers, or a monocyte, macrophage, or polynuclear leukocyte, depending on the identity of the target cell. The reaction is complement-independent.Syndrome: A characteristic symptom complex.Skin: The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.Prospective Studies: Observation of a population for a sufficient number of persons over a sufficient number of years to generate incidence or mortality rates subsequent to the selection of the study group.Single-Domain Antibodies: An immunoglobulin fragment composed of one variable domain from an IMMUNOGLOBULIN HEAVY CHAIN or IMMUNOGLOBULIN LIGHT CHAIN.Polysaccharides, Bacterial: Polysaccharides found in bacteria and in capsules thereof.Kinetics: The rate dynamics in chemical or physical systems.Chromatography, Affinity: A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Iodine Radioisotopes: Unstable isotopes of iodine that decay or disintegrate emitting radiation. I atoms with atomic weights 117-139, except I 127, are radioactive iodine isotopes.Follow-Up Studies: Studies in which individuals or populations are followed to assess the outcome of exposures, procedures, or effects of a characteristic, e.g., occurrence of disease.Prevalence: The total number of cases of a given disease in a specified population at a designated time. It is differentiated from INCIDENCE, which refers to the number of new cases in the population at a given time.Immunosuppressive Agents: Agents that suppress immune function by one of several mechanisms of action. Classical cytotoxic immunosuppressants act by inhibiting DNA synthesis. Others may act through activation of T-CELLS or by inhibiting the activation of HELPER CELLS. While immunosuppression has been brought about in the past primarily to prevent rejection of transplanted organs, new applications involving mediation of the effects of INTERLEUKINS and other CYTOKINES are emerging.Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.Bacterial Vaccines: Suspensions of attenuated or killed bacteria administered for the prevention or treatment of infectious bacterial disease.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Immunochemistry: Field of chemistry that pertains to immunological phenomena and the study of chemical reactions related to antigen stimulation of tissues. It includes physicochemical interactions between antigens and antibodies.

Autoantibodies to RNA polymerases recognize multiple subunits and demonstrate cross-reactivity with RNA polymerase complexes. (1/2145)

OBJECTIVE: To determine the subunit specificity of autoantibody directed to RNA polymerases (RNAP) I, II, and III, which is one of the major autoantibody responses in patients with systemic sclerosis (SSc). METHODS: Thirty-two SSc sera with anti-RNAP antibodies (23 with anti-RNAP I/III, 5 with anti-RNAP I/III and II, and 4 with anti-RNAP II alone) were analyzed by immunoblotting using affinity-purified RNAP and by immunoprecipitation using 35S-labeled cell extracts in which RNAP complexes were dissociated. Antibodies bound to individual RNAP subunits were eluted from preparative immunoblots and were further analyzed by immunoblotting and immunoprecipitation. RESULTS: At least 15 different proteins were bound by antibodies in anti-RNAP-positive SSc sera in various combinations. All 9 sera immunoprecipitating RNAP II and all 28 sera immunoprecipitating RNAP I/III recognized the large subunit proteins of RNAP II and III, respectively. Reactivity to RNAP I large subunits was strongly associated with bright nucleolar staining by indirect immunofluorescence. Affinity-purified antibodies that recognized a 62-kd subunit protein cross-reacted with a 43-kd subunit protein and immunoprecipitated both RNAP I and RNAP III. Antibodies that recognized a 21-kd subunit protein obtained from sera that were positive for anti-RNAP I/III and II antibodies immunoprecipitated both RNAP II and RNAP III. CONCLUSION: Anti-RNAP antibodies recognize multiple subunits of RNAP I, II, and III. Moreover, the results of this study provide the first direct evidence that antibodies that recognize shared subunits of human RNAPs or epitopes present on different human RNAP subunits are responsible for the recognition of multiple RNAPs by SSc sera.  (+info)

Estrogen enhancement of anti-double-stranded DNA antibody and immunoglobulin G production in peripheral blood mononuclear cells from patients with systemic lupus erythematosus. (2/2145)

OBJECTIVE: To study the in vitro effect of estrogen on IgG anti-double-stranded DNA (anti-dsDNA) antibody and total IgG production in peripheral blood mononuclear cells (PBMC) from patients with systemic lupus erythematosus (SLE), in order to elucidate its regulatory role in SLE. METHODS: PBMC from SLE patients and normal donors were cultured with 17beta-estradiol (E2). IgG anti-dsDNA antibodies, total IgG, and cytokine activity in the culture supernatants were measured by enzyme-linked immunosorbent assay. RESULTS: E2 enhanced production of IgG anti-dsDNA antibodies as well as total IgG in PBMC from SLE patients. Anti-dsDNA production in patients with inactive disease was less responsive to E2 than that in patients with active disease. E2 also enhanced total IgG, but not anti-dsDNA, production in the PBMC of normal donors. Antibody production was increased by E2 to a lesser extent in patients' B cells than in their PBMC. Anti-interleukin-10 (anti-IL-10) antibodies partially blocked the E2-induced increase in antibody production in patients' PBMC, but anti-IL-10 had no effect on B cells. E2 increased IL-10 production by patients' monocytes. Exogenous IL-10 acted additively with E2 in increasing antibody production in patients' B cells. CONCLUSION: These results suggest that E2 may polyclonally increase the production of IgG, including IgG anti-dsDNA, in SLE patients' PBMC by enhancing B cell activity and by promoting IL-10 production in monocytes. These findings support the involvement of E2 in the pathogenesis of SLE.  (+info)

Up-regulation of glomerular extracellular matrix and transforming growth factor-beta expression in RF/J mice. (3/2145)

BACKGROUND: RF/J mice were first reported as a murine model of spontaneous glomerulosclerosis by Gude and Lupton in 1960, but the precise histologic characteristics and immunopathological background of this mouse have not been investigated further. METHODS: Measurements of serum levels of immunoglobulins, anti-single strand DNA (anti-ss-DNA) antibody, complement (C3), and circulating immune complex (IC) were performed. Analyses of glomerular histological and immunopathological lesions in association with the detection of mRNA expression of collagen IV, TGF-beta, matrix protein turnover related enzymes, matrix metalloproteinase-2 (MMP-2), tissue inhibitor of metalloproteinase-2 (TIMP-2) and platelet-derived growth factor (PDGF) were also performed in young (10-week-old) and elderly (60-week-old) RF/J mice with age-matched BALB/C mice as the controls. RESULTS: High levels of serum IgA and IgG from as early as 20 weeks of age were noted in the RF/J mice. Serum anti-ss-DNA antibody of aged RF/J mice increased up to 23% of that of aged MRL-lpr/lpr mice, and serum C3 concentration significantly decreased with age, reaching lower levels than that of BALB/c mice. IgA-IC levels were significantly high compared to BALB/C mice both in the early and late stages of life, whereas IgG-IC levels were high only in mice younger than 20 weeks. Semiquantitative and quantitative analyzes of renal histopathological findings revealed significantly marked and age-related mesangial matrix expansion in RF/J mice, with increasing frequency of global glomerular sclerosis and tubulointerstitial damage. On the other hand, although precise measurements of glomerular cell numbers also showed an apparent augmentation in both young and old RF/J mice compared to BALB/C mice, glomerular cellularity decreased with age in RF/J mice. Immunohistochemical study revealed massive immunoglobulin deposition from a young age in association with significantly higher accumulation of matrix proteins, such as types I and IV collagen and laminin from the early stage of life. In addition, in these glomeruli, transforming growth factor-beta1 (TGF-beta1) was highly expressed both in young and old mice. The mRNA expression of MMP-2 was up-regulated only in the early stage of life. Although PDGF mRNA of RF/J mice was significantly up-regulated in the early stage of life, the differences between the mice disappeared in the late stage of life. CONCLUSIONS: These findings suggest that in RF/J mice, an immunopathological background inducing high serum immunoglobulin and IC levels from the early stage of life is closely related to mesangioproliferative glomerular lesions mediated by PDGF, and that development of massive extracellular matrix accumulation in glomeruli was induced by up-regulated expression of TGF-beta with inappropriate regulation of protein turnover-related enzyme production.  (+info)

Cryoglobulinaemia and rheumatic manifestations in patients with hepatitis C virus infection. (4/2145)

OBJECTIVES: To investigate the association of cryoglobulinaemia and rheumatic manifestations in Korean patients with hepatitis C virus (HCV) infection. METHODS: Forty nine Korean patients with HCV infection were recruited. The prevalence, concentration, and type of cryoglobulin (by immunofixation), rheumatoid factor (RF), antinuclear antibody (ANA), and various rheumatological symptoms were investigated and HCV genotype was determined by polymerase chain reaction with genotype specific primer. RESULTS: The prevalence of cryoglobulin was 59% in Korean HCV patients and the concentration of cryoglobulin was 9.8 (7.9) g/l (mean (SD)). The type of cryoglobulinaemia was identified in 23 (80%) of 29 HCV patients with cryoglobulinaemia and they were all type III. There were no differences in age, sex, history of operation and transfusion, proportion of liver cirrhosis between the patients with cryoglobulinaemia and those without cryoglobulinaemia. The frequencies of RF and ANA were 14% and 3.4% respectively in HCV patients with cryoglobulinaemia. There was no difference in HCV genotype between the patients with cryoglobulinaemia and those without cryoglobulinaemia. Clinical features of HCV patients were as follows: arthralgia/arthritis (35%), cutaneous manifestation (37%), Raynaud's phenomenon (8%), paresthesia (44%), dry eyes (22%), dry mouth (10%), oral ulcer (33%), and abdominal pain (14%). However, these rheumatological symptoms did not differ between the two groups. CONCLUSION: Although the rheumatological symptoms were not different between HCV patients with and without cryoglobulinaemia, HCV patients showed various rheumatological manifestations. These result suggests that HCV infection could be included as one of the causes in patients with unexplained rheumatological symptoms.  (+info)

A critical evaluation of enzyme immunoassays for detection of antinuclear autoantibodies of defined specificities. I. Precision, sensitivity, and specificity. (5/2145)

OBJECTIVE: To determine the performance characteristics of enzyme-based immunoassay (EIA) kits for the detection of antinuclear and other autoantibodies of defined specificities. METHODS: Nine manufacturers of EIA kits to detect antibodies of defined specificities participated in a study in which they received coded sera from the Centers for Disease Control and Prevention. These coded sera contained different dilutions of antibody of one specificity mixed with sera containing antibodies of other specificities. The manufacturers were asked to use their standard technology to determine antibody content and send the data to a committee of the International Union of Immunological Societies for analysis. The data were analyzed for sensitivity and specificity in the detection of anti-double-stranded DNA (anti-dsDNA), anti-single-stranded DNA, antihistone, anti-Sm, anti-U1 RNP, anti-SSA/Ro, anti-SSB/La, anti-Scl-70 (DNA topoisomerase I), anticentromere, and anti-Jo-1 antibodies. In addition, replicate samples were included in the coded sera to evaluate the precision of each EIA method. RESULTS: Lack of sensitivity and specificity was most evident in the anti-dsDNA and anti-Sm kits, although 2 kits for anti-dsDNA achieved acceptable sensitivity and specificity. Generally, anti-SSA/Ro, anti-SSB/La, anti-Scl-70, anticentromere, and anti-Jo-1 kits performed well. Many false-positive results were obtained with a multiple myeloma serum containing cryoprecipitates, but multiple myeloma sera without cryoprecipitates presented no problem in the EIA system. Precision, based on evaluation of replicate samples, varied from very good to poor. CONCLUSION: No single manufacturer was clearly superior to others in terms of their products' overall sensitivity, specificity, and precision. Areas that needed improvement were in kits for the detection of antibodies to dsDNA and to Sm antigen. Some EIA kits achieved good sensitivity and specificity. Individual manufacturers were informed of the performance of their respective kits so they could take measures to correct perceived deficiencies and thus improve the reliability of a group of important diagnostic assays used in the evaluation of systemic rheumatic diseases.  (+info)

Influence of ethnic background on clinical and serologic features in patients with systemic sclerosis and anti-DNA topoisomerase I antibody. (6/2145)

OBJECTIVE: To investigate the effect of ethnicity on clinical and serologic expression in patients with systemic sclerosis (SSc) and anti-DNA topoisomerase I (anti-topo I) antibody. METHODS: Clinical and serologic features, as well as HLA class II allele frequencies, were compared among 47 North American white, 15 North American black, 43 Japanese, and 12 Choctaw Native American SSc patients with anti-topo I antibody. RESULTS: The frequency of progressive pulmonary interstitial fibrosis was lower, and cumulative survival rates were better in white compared with black and Japanese patients. Sera of white and black patients frequently recognized the portion adjacent to the carboxyl terminus of topo I, sera of Japanese patients preferentially recognized the portion adjacent to the amino terminus of topo I, and sera of Choctaw patients recognized both portions of topo I. Anti-RNA polymerase II and anti-SSA/Ro antibodies were present together with anti-topo I antibody more frequently in sera of Japanese patients than in sera of white patients. The HLA-DRB1 alleles associated with anti-topo I antibody differed; i.e., DRB1*1101-*1104 in whites and blacks, DRB1*1502 in Japanese, and DRB1*1602 in Choctaws. Multivariate analysis showed that ethnic background was an independent determinant affecting development of severe lung disease as well as survival. CONCLUSION: Clinical and serologic features in SSc patients were strongly influenced by ethnic background. The variability of disease expression in the 4 ethnic groups suggests that multiple factors linked to ethnicity, including genetic and environmental factors, modulate clinical manifestations, disease course, and autoantibody status in SSc.  (+info)

Disparate T cell requirements of two subsets of lupus-specific autoantibodies in pristane-treated mice. (7/2145)

Intraperitoneal injection of pristane induces a lupus-like disease in BALB/c and other non-autoimmune mice characterized by autoantibody production and the development of immune complex disease closely resembling lupus nephritis. Two subsets of autoantibodies are induced by pristane: IgG anti-DNA DNA and -chromatin autoantibodies are strongly IL-6-dependent, whereas IgG anti-nRNP/Sm and -Su antibodies are not. The present studies were carried out to examine the role of T cells in establishing this dichotomy between the production of anti-nRNP/Sm/Su versus anti-DNA/chromatin autoantibodies. Autoantibody production and renal disease were evaluated in athymic (nude) mice treated with pristane. BALB/c nu/nu mice spontaneously developed IgM and IgG anti-single-stranded (ss)DNA and -chromatin, but not anti-nRNP/Sm or -Su, autoantibodies. Pristane treatment increased the levels of IgG anti-chromatin antibodies in nu/nu mice, but did not induce production of anti-nRNP/Sm or -Su antibodies. In contrast, BALB/c nu/+ and +/+ control mice did not spontaneously produce autoantibodies, whereas anti-nRNP/Sm and -Su autoantibodies were induced by pristane in approx. 50% of nu/+ and +/+ mice and anti-DNA/chromatin antibodies at lower frequencies. Nude mice spontaneously developed mild renal lesions that were marginally affected by pristane, but were generally milder than the lesions developing in pristane-treated nu/+ and +/+ mice. The data provide further evidence that two distinct pathways with different cytokine and T cell requirements are involved in autoantibody formation in pristane-induced lupus. This dichotomy may be relevant to understanding differences in the regulation of anti-DNA versus anti-nRNP/Sm autoantibodies in systemic lupus erythematosus, as well as the association of anti-DNA, but not anti-nRNP/Sm, with lupus nephritis.  (+info)

Electrocardiographic abnormalities in a murine model injected with IgG from mothers of children with congenital heart block. (8/2145)

BACKGROUND: It is a widely held view that congenital heart block (CHB) is caused by the transplacental transfer of maternal autoantibodies (anti-SSA/Ro and/or anti-SSB/La) into the fetal circulation. To test this hypothesis and to reproduce human CHB, an experimental mouse model (BALB/c) was developed by passive transfer of human autoantibodies into pregnant mice. METHODS AND RESULTS: Timed pregnant mice (n=54) were injected with a single intravenous bolus of purified IgG containing human anti-SSA/Ro and anti-SSB/La antibodies from mothers of children with CHB. To parallel the "window period" of susceptibility to CHB in humans, 3 groups of mice were used: 8, 11, and 16 days of gestation. Within each group, we tested 10, 25, 50, and 100 microg of IgG. At delivery, ECGs were recorded and analyzed for conduction abnormalities. Bradycardia and PR interval were significantly increased in 8-, 11-, and 16-day gestational groups when compared with controls (P<0.05). QRS duration was not significantly different between all groups. Antibody levels measured by ELISA in both mothers and their offspring confirmed the transplacental transfer of the human antibodies to the pups. CONCLUSIONS: The passive transfer model demonstrated bradycardia, first-degree but not complete atrioventricular block in pups. The greater percentage and degree of bradycardia and PR prolongation in the 11-day mouse group correlates with the "window period" of susceptibility observed in humans. The high incidence of bradycardia suggests possible sinoatrial node involvement. All together, these data provide relevant insights into the pathogenesis of CHB.  (+info)

*Autoantibody

The Antinuclear antibody (ANA) test is often ordered first. ANA is a marker of the autoimmune process - it is positive with a ... Antibodies are produced by B cells in two ways: (i) randomly, and (ii) in response to a foreign protein or substance within the ... An autoantibody is an antibody (a type of protein) produced by the immune system that is directed against one or more of the ... Initially, one B cell produces one specific kind of antibody. In either case, the B cell is allowed to proliferate or is killed ...

*Systemic lupus erythematosus

Subtypes of antinuclear antibodies include anti-Smith and anti-double stranded DNA (dsDNA) antibodies (which are linked to SLE ... Antinuclear antibody (ANA) testing and anti-extractable nuclear antigen (anti-ENA) form the mainstay of serologic testing for ... These are most commonly anti-nuclear antibodies and they result in inflammation. Diagnosis can be difficult and is based on a ... These antibodies clump into antibody-protein complexes which stick to surfaces and damage blood vessels in critical areas of ...

*Drug-induced lupus erythematosus

Antinuclear antibodies are usually positive in drug induced Lupus. Anti-Neutrophil Cytoplasmic antibodies (ANCA) can also be ... Furthermore, Anti-Histone antibodies can also be positive in drug induced lupus. Anti-Histone antibodies are positive in up to ... creating antinuclear antibodies leading to an immune response. Further studies on the interactions between oxidants and ... Anti-histone antibodies in 95% of cases These signs and symptoms are not side effects of the drugs taken which occur during ...

*Anti-nuclear antibody

Anti-nuclear ribonucleoprotein (anti-nRNP) antibodies, also known as anti-U1-RNP antibodies, are found in 30-40% of SLE. They ... anti-Sm antibodies, anti-nRNP antibodies, anti-Scl-70 antibodies, anti-dsDNA antibodies, anti-histone antibodies, antibodies to ... Antinuclear antibodies (ANAs, also known as antinuclear factor or ANF) are autoantibodies that bind to contents of the cell ... This pattern is associated with anti-dsDNA antibodies, antibodies to nucleosomal components, and anti-histone antibodies. There ...

*Anti-p62 antibodies

Invernizzi P, Selmi C, Ranftler C, Podda M, Wesierska-Gadek J (2005). "Antinuclear antibodies in primary biliary cirrhosis". ... 2003). "Profile and clinical significance of anti-nuclear envelope antibodies found in patients with primary biliary cirrhosis ... Anti-p62 antibodies (AP62A) are found in a primary biliary cirrhosis. The p62 protein is also more frequent in Stage IV primary ...

*List of autoimmune diseases

Ehrenstein MR (August 1999). "Antinuclear antibodies and lupus: causes and consequences". Rheumatology. 38 (8): 691-3. doi: ... I. A clinical analysis of 55 anti-Yo antibody-positive patients". Neurology. 42 (10): 1931-7. doi:10.1212/wnl.42.10.1931. PMID ... Franceschini F, Cavazzana I (February 2005). "Anti-Ro/SSA and La/SSB antibodies". Autoimmunity. 38 (1): 55-63. doi:10.1080/ ... "Possible Source of Kawasaki Disease Found". Wisnieski JJ, Naff GB (September 1989). "Serum IgG antibodies to C1q in ...

*Dermatomyositis

... especially antinuclear antibodies (ANA). Around 80% of people with DM test positive for ANA and around 30% of people have ... anti-synthetase antibodies), including antibodies against Histidine-tRNA ligase (also called Jo-1); antibodies to signal ... recognition particle (SRP); and anti-Mi-2 antibodies. Magnetic resonance imaging may be useful to guide muscle biopsy and to ... myositis-specific autoantibodies which include antibodies to aminoacyl-tRNA synthetases ( ...

*Levamisole-induced vasculitis

... antinuclear antibodies were negative and p-ANCA was reactive. Coagulation studies were within normal limits. There was an ...

*Scleromyositis

Blood testing includes screening for the positive antinuclear antibody. Patients have symptoms of both systemic scleroderma and ... Vandergheynst F, Ocmant A, Sordet C, Humbel RL, Goetz J, Roufosse F, Cogan E, Sibilia J (2006). "Anti-pm/scl antibodies in ... Autoantibodies often found in these patients are the anti-PM/Scl (anti-exosome) antibodies. The symptoms that are seen most ... There is still unanswered question of which antibodies will best treat connective tissue diseases. In some cases scleromyositis ...

*Anti-Hu associated encephalitis

Graus F, Cordon-Cardo C, Posner JB (1985). "Neuronal antinuclear antibody in sensory neuronopathy from lung cancer". Neurology ... Nearly all people with the condition have anti-Hu antibodies in their serum. The antibody is produced by the body as an immune ... However, the injection of the antibodies into mice did not produce any disease, and the deposition of antibody was often not at ... Pohley I, Roesler K, Wittstock M, Bitsch A, Benecke R, Wolters A (2015). "NMDA-receptor antibody and anti-Hu antibody positive ...

*Nuclear dots

Pawlotsky JM, Andre C, Metreau JM, Beaugrand M, Zafrani ES, Dhumeaux D (1992). "Multiple nuclear dots antinuclear antibodies ... They are also observed at higher frequencies in subacute sclerosing panencephalitis in these instances antibodies to measles ...

*Immunoglobulin E

Permin H, Wiik A (1978). "The prevalence of IgE antinuclear antibodies in rheumatoid arthritis and systemic lupus erythematosus ... In the second approach, antibodies specific for a domain of 52 amino acid residues, referred to as CεmX or M1' (M1 prime), ... "Antibody structure". Archived from the original on September 6, 2008. Erb KJ (2007). "Helminths, allergic disorders and IgE- ... Immunoglobulin E (IgE) is a type of antibody (or immunoglobulin (Ig) "isotype") that has only been found in mammals. IgE is ...

*HLA A1-B8-DR3-DQ2

Other genes in the region, C4A-null and TNF may associated with autoimmune hepatitis The appearance of anti-nuclear antibodies ... Christian N, Smikle MF, DeCeulaer K, Daniels L, Walravens MJ, Barton EN (March 2007). "Antinuclear antibodies and HLA class II ... of these half had anti-transglutaminase antibodies, but few had endomysial antibody. This could indicate an association with ... DR3 is found to correlate with anti-Ro/La antibodies in SLE. HLA-DR3 has been consistently observed at high frequencies in ...

*Intermittent hydrarthrosis

Rheumatoid factor, cyclic citrullinated peptide antibodies and antinuclear antibodies are usually negative in IH. Palindromic ... 2007). "An unexpectedly high frequency of MEFV mutations in patients with anti-citrullinated protein antibody-negative ...

*Anti-cardiolipin antibodies

"Antinuclear antibody, lupus anticoagulant, and anticardiolipin antibody in women with idiopathic habitual abortion. A ... They are a form of anti-mitochondrial antibody. In SLE, anti-DNA antibodies and anti-cardiolipin antibodies may be present ... Anti-cardiolipin antibodies (ACA) are antibodies often directed against cardiolipin and found in several diseases, including ... Only a subset of autoimmune anti-cardiolipin antibodies bind Apo-H, these anti-apolipoprotein antibodies are associated with ...

*Robert Ira Lewy

Lewy, RI (1995). "Antinuclear Antibodies, Lipid Disturbances and Central Nervous System Imaging Abnormalities in Silicone ...

*Caplan's syndrome

Rheumatoid factor, antinuclear antibodies, and non-organ specific antibodies may be present in the serum. Silicosis and ...

*Rapidly progressive glomerulonephritis

The presence of anti-Glomerular basement membrane (GBM) antibodies suggests type I RPGN; antinuclear antibodies (ANA) may ... In addition to the anti-GBM antibodies, some cases of type I RPGN are also associated with antibodies directed against the ... The antibodies are directed against a particular protein found in the GBM, type IV collagen, specifically the noncollagenous ... The majority of type I disease, however, features anti-GBM antibodies alone; these cases are considered idiopathic. RPGN caused ...

*Acute intermittent porphyria

Allard SA, Charles PJ, Herrick AL, McColl KE, Scott JT (1990). "Antinuclear antibodies and the diagnosis of systemic lupus ... Porphobilinogen Porphobilinogen deaminase Sodium Potassium Anti-nuclear antibody Creatinine Creatine kinase White blood cell ...

*RA33

Hassfeld et al.: Demonstration of a new antinuclear antibody (anti-RA33) that is highly specific for rheumatoid arthritis. ... The pathogenic role of anti-RA33 antibodies is not fully understood. Anti-RA33 antibodies and T cells directed against RA33 ... However, anti-RA33 antibodies are not associated with significant bone erosions or disease activity. In the absence of ... Anti-RA33 antibodies can be easily detected by immunoblotting employing crude nuclear extracts or the recombinant antigen. ...

*Neutrophil extracellular traps

NETs have also been associated with the production of IgG antinuclear double stranded DNA antibodies in children infected with ... "Cytokine-associated neutrophil extracellular traps and antinuclear antibodies in Plasmodium falciparum infected children under ... and complement receptors with various ligands such as antibodies, PMA, and so on. The current understanding is that upon ...

*Anti-dsDNA antibodies

... are a group of anti-nuclear antibodies (ANA) the target antigen of which is double stranded DNA. Blood ... Animal tissue was the first substrate for immunofluorescent detection of antinuclear antibodies and has been in use since the ... The first evidence for antinuclear antibodies arose in 1948 when Hargraves, Richmond and Morton discovered the LE cell. These ... Kumar Y, Bhatia A, Minz RW (2009). "Antinuclear antibodies and their detection methods in diagnosis of connective tissue ...

*Cell nucleus

Pattern of Antinuclear Antibody, and Complement-Fixing Antibodies to DNA in Sera from Patients with Systemic Lupus ... These are known as anti-nuclear antibodies (ANA) and have also been observed in concert with multiple sclerosis as part of ... S Barned; AD Goodman; DH Mattson (1995). "Frequency of anti-nuclear antibodies in multiple sclerosis". Neurology. 45 (2): 384- ... Antibodies to certain types of chromatin organization, in particular, nucleosomes, have been associated with a number of ...

*Crithidia luciliae

... a type of anti-nuclear antibody. Anti-nuclear antibodies are a common feature in SLE and anti-dsDNA antibodies are highly ... "Guidelines for clinical use of the antinuclear antibody test and tests for specific autoantibodies to nuclear antigens. ... The kinetoplast found in C.luciliae allows them to be used for the detection of anti-dsDNA antibodies, ... the organelle can be used to detect anti-dsDNA antibodies, a common feature of the disease. C.luciliae is a eukaryotic single- ...

*HLA A1-B8 haplotype

The association with viral hepatitis was subsequently demonstrated and patients with antinuclear antibodies were more likely to ... Though, the association of A1 with autoimmune hepatitis with no anti-viral antibody was stronger than with chronic active ...

*Anti-Scl-70 antibodies

... is an anti-topoisomerase antibody-type of anti-nuclear autoantibodies, seen mainly in diffuse systemic scleroderma (with a ... Anti Scl-70 antibodies are associated with more severe scleroderma disease. The etymology of scl-70 consists of an abbreviation ... de Rooij DJ, Van de Putte LB, Habets WJ, Van Venrooij WJ (1989). "Marker antibodies in scleroderma and polymyositis: clinical ... of the antibodies. Guldner HH, Szostecki C, Vosberg HP, Lakomek HJ, Penner E, Bautz FA (1986). "Scl 70 autoantibodies from ...

*Kikuchi disease

Antinuclear antibodies, antiphospholipid antibodies, anti-dsDNA, and rheumatoid factor are usually negative, and may help in ... Clinical findings sometimes may include positive results for IgM/IgG/IgA antibodies. For other causes of lymph node enlargement ... In addition, serologic tests including antibodies to a host of viruses have consistently proven noncontributory and no viral ...
Looking for Antinuclear Antibody Test? Find out information about Antinuclear Antibody Test. antinuclear antibody Explanation of Antinuclear Antibody Test
CLINICAL RESEARCH STUDY The Clinical Utility of a Positive Antinuclear Antibody Test Result Aryeh M. Abeles, MD, Micha Abeles, MD Division of Rheumatology, University of Connecticut Health Center, Farmington.
The serological diagnosis of juvenile rheumatoid arthritis (JRA) is difficult, with only 7-10% of patients 19S IgM rheumatoid factor positive. About 60-70% of patients are positive for hidden 19S IgM rheumatoid factor, but this test requires serum separation and is not available in most laboratories. Antiperinuclear factor has been described in both seropositive and seronegative adult patients with rheumatoid arthritis, but has not been thoroughly evaluated in children with JRA. This study determined the diagnostic sensitivity and specificity of antiperinuclear factor in patients with JRA. Serum samples from 64 children with JRA, 24 with systemic lupus erythematosus (SLE), and 24 control subjects were tested for the presence of antiperinuclear factor. A total of 10 (83%) of seropositive, polyarticular onset and six (37%) of seronegative, polyarticular onset patients with JRA were positive for antiperinuclear factor. The occurrence of antiperinuclear factor in five (19%) with pauciarticular onset ...
Antinuclear Antibody Test Market by Product (Reagents & Assay Kits, Systems, Software, Services), Technique (Immunofluorescence, Elisa, Multiplex), Disease (Rheumatoid Arthritis, SLE), End User (Clinical Labs, Hospitals) - Forecast to 2021 is a market research report available at US $5650 for a Single User PDF License from RnR Market Research Reports Library.
The antinuclear antibody (ANA) test is widely used as a serological marker of autoimmune disease. Antinuclear antibodies are immunoglobulins or antibodies that bind to one or more antigens expressed within the nucleus of human cells. Used selectively, the ANA test can be a useful laboratory tool to help confirm or exclude the diagnosis of systemic rheumatic disease. However, the relatively high prevalence of ANAs in other inflammatory conditions, as well as healthy individuals, can make a positive result difficult to interpret.
Health, ...The presence of antinuclear antibodies (ANA) indicates the possibility...The immune system is responsible for protecting the body against forei...The Brazilian research team led by Luis Andrade MD PhD from the Fe... The ANA-HEp-2 test is positive in a sizable portion of the general po...,Accurate,interpretation,of,antinuclear,antibodies,test,key,to,confirming,autoimmune,disease,medicine,medical news today,latest medical news,medical newsletters,current medical news,latest medicine news
Autoantibodies to nuclear and cytoplasmic constituents are characteristically present in connective tissue diseases or "autoimmune" processes. The lupus erythematosus (LE) cell test was the first practical demonstration of an antinuclear antibody that was found to be in the gamma globulin fraction of sera from patients with systemic lupus erythematosus (SLE) (1). With the advent of the indirect fluorescent antibody technique, more antinuclear factors became demonstrable. Antinuclear antibodies are present in the immunoglobulin classes, gamma 2 (gamma G or IgG), gamma 1A (gamma A or IgA), and gamma 1M (gamma M or IgM) (2) and lack organ and species specificity. Thus ...
The incidence of patients with positive antinuclear antibody test results rose during three years of treatment with hydralazine. At the end of that period over half of the patients (both rapid and slow acetylators) had titres exceeding 1/20, but the
Aims: Positive serum antinuclear antibody (ANA) is present in a number of patients with chronic hepatitis C virus (HCV) infection. This study aimed to evaluate the prevalence of ANA in patients with chronic hepatitis C (CHC) and to elucidate its clinical implications in virological and histological characteristics of CHC infection.. Methods: A total of 614 CHC patients were enrolled in this prospective, hospital-based study. The serum levels of aspartate aminotransferase, alanine aminotransferase and ANA, and HCV genotype, HCV RNA level, and histological activity index scores for liver histopathology, were determined.. Results: The prevalence of positive ANA (titre ,1:40) was 35.0%. Women had a significantly higher prevalence than men (41.2 vs 31.0%; p = 0.012). Patients positive for ANA were significantly older (mean (SD), 53.7 (10.5) vs 49.7 (11.3) years; p,0.001) and had higher mean (SD) alanine aminotransferase levels (186.9 (178.8) vs 155.50 (113.5) IU/l; p,0.001) and lower mean (SD) HCV ...
The ANA test has a specificity of around 95 percent, meaning that of 20 people who test negative, 19 actually do not have lupus, but one has tested falsely negative and may have lupus. Its sensitivity is even less, meaning that more than one person out of 20 will test falsely positive but will not have lupus. False positive ANAs are fairly common and occur more frequently in older people, people with other autoimmune diseases, with viral infections, some chronic inflammations, and after taking some medications. Medications notorious for producing positive ANA tests are procainamide, used for some heart arrhythmias, and hydralazine, which used to be frequently prescribed to treat high blood pressure. Positive ANAs may persist for years after someone has taken these drugs.. The other common belief which I want to debunk here is that it is always important, even critical, to treat a disease early in the hopes of producing a cure. This belief probably stems from the emphasis we place on detecting ...
Hi I had a positive antinuclear antibody test that came back positive with 160 homogeneous when it is supposed to be at 40 I dont know my doctor has tested me for viral infections which came back negative what is clear to the doctor is that something is fighting me in my body attacking my joints Im going to a rhemuatologist but I dont know what to expect they will test me for lupus and so forth but the doctor doesnt think that is it and tested negative for rhuematoid arthiritis but I have some type of arthiris now from whatever has been attacking the funny thing is that I had some serious injuries bout three months I think knee and feet and fingers knuckles now since about a month ago they seem to be inflammatory not all at once though so far swelling is down on my knees but its not in my fingers and hand has anyone had these symptoms the only family history I have with diseases is leukemia which took my mums brother at an early age and Alzheimers on my dads side no arthiritic conditions ...
My latest blood work came back with a positive ana. I was wondering if anyone else had the same? I have an appointment with a rheumatologist to look...
Objective: An Italian multicentre study was promoted in order to assess the accuracy of four anti-double-stranded DNA (dsDNA) antibody assays for SLE diagnosis and monitoring. Methods: Two hundred and twenty-three patients with established SLE according to ACR classification criteria were enrolled from 9 centres. They included 59 patients at first evaluation (disease duration ,12 months) and 164 with longer disease duration (median disease duration 120 months). The sera from 55 healthy subjects and 161 patients with rheumatic, infectious or neoplastic diseases were tested as controls. SLE activity was measured by ECLAM score. Anti-dsDNA antibodies were detected in serum by means of FarrzymeTM assay, fluoroenzymeimmunoassay (EliATM), Crithidia luciliae indirect immunofluorescence (CLIFT) or Farr radioimmunoassay (Farr). Cut-off values of quantitative assays were chosen by ROC curves analysis. Statistics were conducted by SPSS software package. Results: Sensitivity for SLE diagnosis ranged between ...
An ANA test detects antinuclear antibodies in your blood. Your immune system normally makes antibodies to help you fight infection. In contrast, antinuclear antibodies often attack your bodys own tissues - specifically targeting each cells nucleus.
The results of antinuclear antibody tests using the indirect immunofluorescence technique may be reported as a description of the pattern and the intensity of fluorescence obtained at a certain dilution. If quantitative results are required titration is necessary. Such titrations may vary greatly between different laboratories. The present study involving 26 laboratories shows an improvement of interlaboratory comparability for the homogeneous fluorescence pattern when a common reference serum is used. Cultured cells as substrate appear to give better quantitative agreement than rat liver sections. National reference sera should be standardised in items of the appropriate WHO reference preparation. ...
CDC Split Type: (blank) WAES0703USA03739. Write-up:Information has been received from a physician concerning his daughter, a female patient, with a penicillin allergy, who on 15-DEC-2006 was vaccinated with the first dose, 0.5ml, IM, of Gardasil. There was no concomitant medication. On approximately 15-JA"N-2007 (/" 15-JAN-2007 (" one month later/") later") the physicians physicians daughter experienced /"numbness "numbness and pain in her right foot and leg./" leg." The physician reported that his daughter was examined by both a neurologist and a rheumatologist, and was not certain if she was /"experie "experiencing significant disability," but was waiting to see if the numbness and pain resolved. The physician reported there was "some improvement." Additional information has been requested. 01-Jan-2007 magnetic resonance imaging, Negative, 01-Jan-2007 serum C-reactive protein test, Negative, 01-Jan-2007 serum antinuclear antibodies test, Positive ...
OBJECTIVE: To examine the presence of serum antinuclear autoantibodies in a healthy population. METHODS: Serum of 500 normal blood donors between 18 and 60 years of age were teste..
Yes, Im seeing an MFM as well as my OB, I am taking LDA. They just said that ANA positive without any other factors and all other normal blood work could be mean that I will develop some sort of disease or problem down the road, or it could mean nothing at all. They said its not a test typically done on "healthy" people with no cause for testing, so its hard to know the exact numbers but that many healthy people just test positive for it. Its just frustrating because I got pre-e, one week later my son passed away, two days later they tell me I test positive for ANA...and yet they tell me all of these things are completely unrelated. I have two wonderful and caring doctors that I am seeing, but its just hard to accept so many things happening together that are all unrelated and yet all have such low odds of happening to me. I guess I will find it all more believable if this rainbow baby comes home safe and sound and I avoid pre-e ...
(2000) Ciccarelli et al. Multiple sclerosis (Houndmills, Basingstoke, England). We determined whether positive ANA was related to response to rIFNss-1a in 62 relapsing-remitting MS patients. According to the presence of antinuclear antibodies (ANA) at baseline and during the f...
www.MOLUNA.de Case Studies in Systemic Sclerosis [4185368] - A 35- Year Old Woman with Puffy Hands, Raynauds Phenomenon, & Positive Antinuclear Antibody Test.- A 30- Year Old Woman with Puffy Hands, Raynauds Phenomenon, & Carpal Tunnel Syndrome.- A 22- Year Old Woman with Raynauds Phenomenon But No Other Symptoms & No Abnormalities on Examination.- A Young Adult With
The immune system makes an abundance of proteins called antibodies. Antibodies are made by white blood cells (B cells). The antibodies recognize and combat infectious organisms (germs) in the body. Antibodies develop in our immune system to help the body fight infectious organisms. When an antibody recognizes the foreign proteins of an infectious organism, it recruits other proteins and cells to fight off the infection. This cascade of attack is called inflammation.. Sometimes these antibodies make a mistake, identifying normal, naturally-occurring proteins in our bodies as being "foreign" and dangerous. When these antibodies make incorrect calls, identifying a naturally-occurring protein (or self protein) as foreign, they are called autoantibodies. Autoantibodies start the cascade of inflammation, causing the body to attack itself. The antibodies that target "normal" proteins within the nucleus of a cell are called antinuclear antibodies (ANA). Most of us have autoantibodies, but typically in ...
FIDIS (BMD, France) is a multiplex analytical flow cytometry system for the detection of antibodies. The aim of this study was to evaluate the FIDIS connective assay system for the detection of antinuclear antibodies (ANA), and to assess the clinical utility of these parameters in the diagnosis of connective tissue diseases. The FIDIS system simultaneously measures IgG antibodies directed at dsDNA, Ro, La, RNP, Sm, Jo-1, Scl-70, rRNP, and CENP-B. ...
A positive antinuclear antibodies, or ANA, test result can change to negative, especially in people who have short-term viral infections, according to the American College of Rheumatology. However,...
The antinuclear antibody (ANA) test is commonly used to look for autoantibodies that attack components of your cells nucleus, or "command" center, triggering autoimmune disorders like lupus. 95% of people with lupus test positive for ANA, but a number of other, non-lupus causes can trigger a positive ANA, including infections and other autoimmune diseases. The ANA test simply provides another clue for making an accurate diagnosis.. For patients with a positive ANA, more tests are usually performed to check for other antibodies that can help confirm the diagnosis. This series of tests, commonly called an ANA panel, checks for the following antibodies: anti-double-stranded DNA, anti-Smith, anti-U1RNP, anti-Ro/SSA, and anti-La/SSB. Some laboratories also include other antibodies in their panel, including antinucleoprotein, anticentromere, or antihistone.. Doctors use these supplemental tests in conjunction with a persons clinical history to help diagnose or rule out other autoimmune disorders. ...
Teague, P O. and Friou, G J., "Antinuclear antibodies in mice. II. Transmission with spleen cells, inhibition or prevention with thymus or spleen cells." (1969). Subject Strain Bibliography 1969. 1389 ...
The ANA test is ordered when someone shows signs and symptoms that are associated with a systemic autoimmune disorder. People with autoimmune disorders can have a variety of symptoms that are vague and non-specific and that change over time, progressively worsen.The antinuclear antibody (ANA) test is used as a primary test to help evaluate a person for autoimmune disorders that affect many tissues and organs throughout the body (systemic) and is most often used as one of the tests to help diagnose systemic lupus erythematosus (SLE). ANA are a group of autoantibodies produced by a persons immune system when it fails to adequately distinguish between "self" and "nonself." They target substances found in the nucleus of a cell and cause organ and tissue damage. Depending on a persons signs and symptoms and the suspected disorder, ANA testing may be used along with or followed by other autoantibody tests. ...
ResearchMoz presents professional and in-depth study of "Autoimmune Disease Diagnostics Market: (By Test Types: Antinuclear Antibody Test, Autoantibody Test, Complete Blood Count, Comprehensive Metabolic Panel, C-Reactive Protein Test, Erythrocyte Sedimentation Rate, Urinalysis and Others; Disease: Graves Disease, Hashimotos Thyroiditis, Rheumatoid Arthritis, Multiple Sclerosis, Systemic Lupus Erythematosus, Type 1 Diabetes, and Others): Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2015 - 2023".. This report on the autoimmune disease diagnostics market studies the current and future prospects of the global market. Autoimmune disease diagnostics market includes various laboratory tests that are performed to diagnose autoimmune disorders. These tests include blood tests for one or more autoantibodies and tests for inflammation. The rising prevalence of autoimmune diseases, increase in health care expenditure globally, rising government initiatives and increasing automation ...
Any antibody to nuclear components is an ANA. Most patients with ANAs do not have SLE, but most people with SLE have ANAs. The most common screening test is IIF on rodent liver or human epithelial (HEp2) tissue,3 although ELISA tests are available.4,5 Lupus erythematous cells simply represent nuclei opsonised by ANAs and are no longer used in diagnosis. Although ANAs are very sensitive for SLE, positive ANAs are common, especially in unwell elderly individuals.6-8 Therefore, ANAs have low PPV for SLE in unselected populations or when present in low titres,6,9 and are not diagnostic. One in three healthy people have detectable ANAs on HEp-2 cells at a screening dilution of 1/40 and one in 20 will be positive at 1/160. HEp-2 cells produce more positive ANAs than rat tissue, and some ANAs (for example, anticentromere antibodies) can only be reliably detected on HEp-2 substrate. Although "ANA negative" SLE is reported,10 it is not clear whether this is the result of a technical artifact or whether a ...
Hi, I have many of the Lupus symptoms but all the test show negative; is possible that I have lupus and all the tests (except anti-nuclear antibodies) show negative result?. The Rheumatologist said it might be Fibromyalgia but i have peeling nails and a little bit of face rash so it doesnt completely match.
Hello, Few of my joints hurt occasionally. My blood test returned: positive ANA, speckled pattern (all other arthritis tests are normal). Two years ago, I had negative ANA, so I assume Im not i...
Question - Chronic pain in abdominal area, tiredness, joint pain, bloating. Tests showed positive ANA, hypothyroid, elevated inflammation. Suggestion?. Ask a Doctor about diagnosis, treatment and medication for Abdominal pain, Ask an Orthopaedic Surgeon
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HSV1 + HSV2 Nuclear antibody [0119] for ICC/IF. Anti-HSV1 + HSV2 Nuclear mAb (GTX36659) is tested in Herpes simplex virus samples. 100% Ab-Assurance.
If ANA is POSITIVE, a reflex ANA titer is always performed. ANA Reflex to additional ENA/dsDNA testing is not an orderable test at Seattle Childrens Hospital. Additional specific tests can be ordered at time of ANA collection, or, alternatively, all ANA serum is saved for 6 months. If dsDNA/ENA testing is desired after initial ANA testing, order as an Add-on Communication in CIS or call laboratory (206) 987-2102.. ...
Mouse monoclonal ds DNA antibody [35I9 DNA] validated for ELISA, IHC, ICC, Dot, ICC/IF. Referenced in 18 publications and 3 independent reviews. Immunogen…
This test guide provides information about tests that may be useful for the diagnosis of autoimmune diseases. Three screening approaches that use antinuclear antibody and specific antibody tests are discussed.
Anti ds DNA antibody-SLE Anti histone antibody-Drug induced SLE Anti sm antibody/antibody to core protein of small nuclear ribonucleoprotein/anti smith
The immunofluorescent antinuclear antibody (ANA or FANA) test is positive in almost all individuals with systemic lupus (97 percent), and is the most sensitive diagnostic test currently available for confirming the diagnosis of systemic lupus when accompanied by typical clinical findings. When three or more typical clinical features are present, such as skin, joint, kidney, pleural, pericardial, hematological, or central nervous system findings as described above, a positive ANA test confirms the diagnosis of systemic lupus ...
The ssDNA antibody test detects ssDNA and some dsDNA antibodies. As a result, patient samples should also be tested for dsDNA antibodies in order to more accurately determine the level of anti-ssDNA antibodies. By calculating the ratio (ssDNA/dsDNA), a conclusion can be made as to the presence of ssDNA antibodies. A ratio less than or equal to 1.0 indicates the absence of ssDNA antibodies. A ratio greater than 1.0 indicates the presence of ssDNA antibodies ...
It is our premise that JDMS is a distinct disease entity and that the increase in HLA-B8 and -DR3 in JDMS places this disease in the company of other immunopathic disorders. There are conflicting data concerning immunological abnormalities in JDMS , but there appears to be impairment of natural killing and evidence of complement activation. The frequent positive ANA in JDMS raises the speculation of its relationship to the antinuclear antibody, Jo-1, found in some adults with PM, which has specificity for tRNAHis. Most newly diagnosed JDMS patients have antibodies to Coxsackie B which may be related to the pathogenesis of this disease. Specific pathological findings of endothelial cells containing reticulotubular inclusions are associated with vessel occlusion, subsequent obliteration and increased Factor VIII levels in clinically active disease ...
The medical institutes who establish diagnostic standards say that, you probably have Lupus if you have two or more of these symptoms plus a positive ANA (antinuclear antibodies) blood test, OR if you have four or more of these symptoms with no other reason for them ...
ANA also goes up and down all the time. The info and advice in the above posts is excellent. And the funny thing is that I can feel like crap and my ANA will be relatively low, then feel great and its high. So go figure. I hope thatyour other test are normal and that its just the way you are and not any illness. My ANA was elevated for over a decade and probably longer with no symptoms, then I developed diabetes with NASH and endocrine problems and all of that was still unrelated to my ANA according to my docs. then I developed sjogrens syndrome, a relatively mild form of autoimmune disease. I believe the interferon I took caused my autoimmune disease to become active. I think otherwise I would have continued to havean elevated ANA with no symptoms for the rest ofmy life otherwards ...
The purpose of this study was to evaluate the precision and accuracy of a commercial multiplexed kit for the measurement of 9 anti-nuclear antibodies (ANAs; anti-SS/A, anti-SS/B, anti-Sm, anti-RNP, anti-Jo-1, anti-Scl-70, anti-dsDNA, anti-Centromere B, and anti-Histone), and to compare these results to a subset of ANAs measured by enzyme-linked immunosorbent assays (ELISA) and immunodiffusion (ID)
The production of auto-antibodies is one of the predominant characteristics of autoimmune disorders. Because IL-2 deficient mice develop autoimmunity, we asked how IL-2 deficiency might impair endogenous mechanisms of B cell tolerance. To this end, we mated BALB/c anti-dsDNA H chain knock-in mice, in which B cells producing anti-dsDNA antibodies are properly regulated, with IL-2 deficient mice and assessed the phenotype of their offspring. IL-2 deficient mice expressing the anti-dsDNA H chain knock-in allele developed anti-dsDNA antibodies of both IgM and IgG isotypes. Production of these antibodies occurred through the disruption of several mechanisms of endogenous tolerance, including deletion, maturational arrest, and follicular exclusion. In summary, our results suggest that IL-2 plays an important role in regulating B cell tolerance.
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Background EBV infection and the immune response may be involved in the pathogenesis of rheumatoid arthritis (RA). All were reported as titers except BZLF-1 and CMV which were reported as positive or unfavorable. ANA positive samples were excluded. Elevated EBV antibody titers had been defined as top of the 20% (or nearest titer) among handles. Conditional logistic regression analyses modeled RA risk connected with raised EBV WAY-362450 titers or the existence/lack CMV further altered for pack-years smoking cigarettes and alcoholic beverages intake. Outcomes 87 occurrence RA situations had been identified. Mean time for you to RA after bloodstream pull was 6.2 (±3.5) years in NHS and 1.9 (±0.6) years in NHSII. Antibody titers against EBV werent different between pre-RA situations and handles significantly. Conclusions Within this prospective research of females we observed zero association between EBV RA and serologies risk. distributed epitope (exams (to take into account the complementing ...
After washing to remove non-specifically bound antibodies, the substrate is incubated with an anti-human antibody conjugated to fluorescein. When results are positive, a stable three-part complex forms, consisting of fluorescent antibody bound to human antinuclear antibody that is bound to nuclear antigen. This complex can be visualized with the aid of a fluorescent microscope. In positive samples, the cell nuclei will show a bright apple-green fluorescence with a staining pattern characteristic of the particular nuclear antigen distribution within the cells. If the sample is negative for ANA, the nucleus will show no clearly discernible pattern of nuclear fluorescence. The cytoplasm may demonstrate weak staining while the non-chromosome region of mitotic cells demonstrates brighter staining ...
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Human epithelial cell (HEp-2) substrate slides, reagents, controls, and supplies for antinuclear antibody immunofluorescence assays (8 x 6 wells)
Comp Metabolic Panel, Lipid Panel, CBC with Diff w/ Pit, Amylase, Antinuclear Antibodies, CRP, Sed Rate, TSH (3rd Generation), Uric Acid, Urinalysis, Vitamin D (25-Hydroxy), and Testosterone Free Lipose. I will also be taking a chest X-Ray as it can be difficult to breathe while inflamed. All tests turned out well (some low and high areas but all very near the acceptable range) except my Sed Rate / C-Reactive Protein. Both are very high (SED rate now at 101, normal 0-15 range). Also, my blood pressure was lower than expected. Last visit a week ago was 118/70, now it was 94/60 - this did not concern the doctor, but I do need to watch ...
Satoh, M., Chan, E.K., Ho, L.A., Rose, K.M., Parks, C.G., Cohn, R.D., Jusko, T.A., Walker, N.J., Germolec, D.R., Whitt, I.Z., Crockett, P.W., Pauley, B.A., Chan, J.Y., Ross, S.J., Birnbaum, L.S., Zeldin, D.C., Miller, F.W. . Prevalence and sociodemographic correlates of antinuclear antibodies in the United States ...
Satoh, M., Chan, E.K., Ho, L.A., Rose, K.M., Parks, C.G., Cohn, R.D., Jusko, T.A., Walker, N.J., Germolec, D.R., Whitt, I.Z., Crockett, P.W., Pauley, B.A., Chan, J.Y., Ross, S.J., Birnbaum, L.S., Zeldin, D.C., Miller, F.W. . Prevalence and sociodemographic correlates of antinuclear antibodies in the United States ...
Hi, my name is Kristi. I was just diagnosis with CREST. My sister has LUPUS (involving kidneys) and after participating in a LUPUS genetic study for her, they found a high positive ANA in me. I went to my general practitioner and they did another ANA and it was negative. I went to a Rheumatologist and she told me they have to specifically ask for the anticentromere pattern of ANA or they will not test for this rare pattern and that is why I got the negative result the second time. The rheumatologist repeated the ANA once more on me, specifically asking for the centromere pattern and it came back with a high positive of 1:1,280. She diagnosed me with CREST due to my raynauds and daily severe heart burn. I also have the beginning skin changes on my fingers. She ordered the lung PFT, Echocardiogram heart test and a Barrium swallow to check the condition of my esophagus. She thinks it is limited, not diffuse, so I guess I am lucky there. Any advice or encouragement is welcome. Thanks, Kristi ...
According to the present findings, SD produced an immediate and short-lasting effect, manifested by an earlier triggering of the disease, which was reflected by more cases of positive ANA. Nonetheless, SD did not affect the evolution or severity of the disease, according to the proteinuria and longevity data.. Similar to other studies (7, 25), the onset and evolution of the disease was based on the presence and amount of ANA. This is an extremely sensitive and specific parameter, and, contrary to human beings, it is a measurement of disease activity.. One of the most replicable effects of SD in rats is the progressive increase of energy expenditure, manifested by augmented food intake and weight loss (3, 11, 16). Contrary to what is observed in rats, SD did not produce weight loss in NZB/NZW F1 mice. Both control and SD mice gained weight after the manipulation compared with their pre-SD body weight. Although we did not assess food intake, it could be hypothesized that this distinct effect of SD ...
Mouse monoclonal CD45RO antibody [UCHL-1] validated for WB, IHC, Flow Cyt and tested in Human and Rat. Referenced in 3 publications. Immunogen corresponding to…
Nuclear Antigen antibody [58-15] for ICC/IF, IHC-P. Anti-Nuclear Antigen mAb (GTX39529) is tested in Human, Rat samples. 100% Ab-Assurance.
ENAP. If ENA or any of the components of the CLS ENA test are ordered on CLS requisition, even if "Mitogen Advanced Diagnostic Lab" or "MADL" is also written on the CLS requisition, order ENAP ...
Double-stranded DNA antibody answers are found in the Guide to Diagnostic Tests powered by Unbound Medicine. Available for iPhone, iPad, Android, and Web.
Hello all, I am not sure if anyone can help me but I recently had some blood work done and had some questions about the results. I had a positive ANA a couple of years ago, but it was never tite...
However, just because a soldier decides he/she no longer likes the USA, does not mean that he/she is actually going to do anything about it. And just because you have an ANA, does not mean that your body is being attacked. In fact most ANAs do not actually do anything. In one study, low level ANAs (titers of 1:40) occurred in 32% of normal patients, moderate level ANAs (titers of 1:80) occurred in 13% of normal patients, and high level ANAs (titers of 1:320) occurred in 3% of normal patients. Up to 37% of those over 65 can have a positive ANA ...
Hi Dr Coulam, I am wondering if you think IVIg would be a prescribed treatment for one or any of the following: anti-thyroid antibodies anti-phosphatidic antibodies anti-nuclear antibodies (1:40 titre) If not IVIg, would prednisone be sufficient? Any other thoughts of treatment options?
Number of Patients With : Antinuclear and/or Anti-SSa and/or Anti-SSb and/or Anti-RNP and/or Anti-DNA and/or Anti-Sm and/or Anticardiolipid and/or Anti β2Gp1 and/or Antiganglioside Autoantibodies (Genetics Analyses From Blood Samples ...
History: Young teen-age male complained of red scaly plaques on his nose of 6 months duration. The scales were adherent to the skin and their forceful detachment revealed carpets tack appearance. No associated other skin problem or systemic disease. The anti-nuclear antibody (ANA) was positive. ...
CDC Split Type: WAES0704USA03537. Write-up:Information has been received from a physician concerning a 19 year old female patient who was vaccinated with a dose of Gardasil. The patient experienced joint pain after getting the injection. Unspecified medical attention was sought. Patients outcome was unknown. Additional information has been requested. 07/06/07 This is in follow-up to report(s) previously submitted on 5/14/2007. Initial and follow up information by telephone has been received from a physician concerning a 19 year old female patient who was vaccinated with a first and second dose of GARDASIL. The patient experienced joint pain after getting her second injection. Follow up information from the physician indicated that the patient had a positive ANA and an increased sed rate and he was trying to rule out the possibility of lupus. The physician requested additional information about GARDASIL and lupus and whether or not the patient should receive her third injection. On ...
Well I finally asked my doctor to send me a copy of my blood results so I know what they are talking about. So here are the results: ANA titer 1:10240 Scl...
ENA Screen ELISA is an indirect solid phase ELISA for the screening of IgG class autoantibodies against extractable nuclear antigens (ENA) in human serum or plasma. (KA1106) - Products - Abnova
Anti-Histone H4 (acetyl K8) antibody - ChIP Grade (ab15823) has been cited in 31 publications. References for Human, Mouse, Chk, Sc, Ce in ChIP, EM, ICC/IF, WB
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SUMMARY. Twenty patients with unexplained pulmonary fibrosis and circulating antinuclear factor were identified. Diseases commonly associated with antinuclear factor, such as systemic lupus erythematosus or scleroderma, were excluded. Half of the patients showed typical restrictive ventilatory defects and X-ray evidence of severe fibrotic changes. The remaining patients predominantly had evidence of obstructive airway diseases.. Four lung biopsies were available. Three showed histological evidence of interstitial pulmonary fibrosis. Immunofluorescent study of one of the biopsy sections with pulmonary fibrosis from a patient with only IgM serum antinuclear factor showed IgM in the alveolar septa. Relatively little fibrosis was present in immunoglobulin-laden areas whereas no immunoglobulin could be demonstrated in the adjacent alveolar septa showing marked fibrosis. Seven out of 10 patients with X-ray evidence of severe interstitial pulmonary fibrosis and typical restrictive ventilatory defects ...
Objective: To investigate the rote of anti-nucleosome antibodies (AnuA) in systemic lupus erythematosus (SLE).. Methods: IgG anti-nucleosome antibodies were detected by enzyme-linked immunosorbent assay (ELISA) in the sera of 233 SLE and 220 other rheumatic diseases′ patients as well as 31 health controls. The patients were also evaluated for clinical and biological parameters.. Results: (1) Out of 233 SLE patients, 144 (61.8%) were seropositive for AnuA, which was significantly higher than that of patients with other rheumatic diseases [2.7% (6/220), P < 0.001]; the sensitivities and specificities of AnuA in SLE were 61.8% and 97.6%, respectively. (2) The positive rated of AnuA in SLE lacking of anti-DNP, anti-cmDNA, anti-Sm and anti-dsDNA antibodies were 57.1%, 55.9%, 62.4% and 51.2%, respectively. (3) The frequency of the fever, skin rash, and arthralgia were significantly higher in 144 positive AnuA SLE than those in AnuA negative SLE (P < 0.05). The frequency of leukopenia, elevations of ...
Limited data is available regarding the clinical manifestations and pattern of Systemic Lupus Erythematosus (SLE) in Sudan. This study aimed to determine the clinical manifestations and Antinuclear Antibodies (ANA) profile among Sudanese adults with SLE and lupus nephritis (LN).
IgMa anti-single-stranded DNA (anti-ssDNA), antihistone, antichromatin, and anti-Sm autoantibodies (autoAb) in VH3H9R/VLκ8R.B6, VH3H9R/VLκ8R.B6.129chr1b129/
Antinuclear antibodies are associated with rheumatic diseases including Systemic Lupus Erythematous (SLE), mixed connective tissue disease, Sjogrens syndrome, scleroderma, polymyositis, CREST syndrome, and neurologic SLE. ...
Kumar, V.; Krasny, S.; Beutner, E.H., 1985: Specificity of the Crithidia luciliae method for detecting anti-DNA antibodies. Effect of absorption for lipoproteins
Abstract OBJECTIVE/HYPOTHESIS: The aim of this prospective study is to evaluate the possible association between Ménières disease (MD) and autoantibodies. METHODS: Fifty-five patients with definite MD (51 unilateral and 4 bilateral) were matched with 55 patients with unilateral vestibular paresis without cochlear involvement and 55 healthy subjects. Blood samples were collected from all study subjects for the determination of serum TSH, free triiodothyronine, free thyroxine, anti-TSH receptor antibody, antithyroperoxidase antibody, antithyroglobulin antibody and of antibodies to non-organ-specific antigens, namely antinuclear antibodies, antibodies to extractable nuclear antigens and antineutrophilic cytoplasmic antibodies. RESULTS: Thirty-three subjects (60%) of the MD group had 1 or more elevated serum autoantibody levels, both organ and non-organ specific; 16 patients (29.1%) with unilateral vestibular paresis had 1 or more elevated serum autoantibody levels, while 13 healthy subjects ...
Tannen, R H. and Weber, W W., "Antinuclear antibodies related to acetylator phenotype in mice." (1980). Subject Strain Bibliography 1980. 457 ...
A progressively wide range of studies exist into the association of Anti-Nuclear Antibodies (ANAs) with Autoimmune Rheumatic Diseases (ARDS), such as Systemic Lupus Erythematosus (SLE), Sjogrens Syndrome (SjS), Systemic Scleroderma (SS), Rheumatoid Arthritis (RA), Reynauds phenomena (RP) to name but a few of the more serious and debilitating ARDs1-4. It is thought that a single base mutation in the gene responsible for anti-Phosphotidylcholine (aPTC), (an IgM Natural Autoantibody (NAb) responsible for clearance of cellular debris) is a likely mechanism by which ANA pathogenesis may originate5. Visualization of these antibodies through Indirect Immuno-Fluorescence (IIF) has long remained the principle detection path, the "Gold Standard". The objective of this study is to investigate the replacement of IIF with ELISA as a screening/diagnostic method in the ongoing effort to eliminate the time-consuming and subjective nature of IIF; ELISA being easily adapted to automation. 350 routine anonymised ...
Prolactin is a peptide hormone produced by the anterior pituitary gland that is critical in lactation. Prolactin can also be produced by lymphocytes, and both B and T cells express prolactin receptors. These findings have suggested that prolactin has immunomodulatory functions. Studies in spontaneously autoimmune hosts have demonstrated a role for prolactin in augmenting autoreactivity. We chose to analyze prolactin effects on anti-DNA B cells in nonspontaneously autoimmune female BALB/c mice transgenic for the heavy chain of an anti-DNA antibody. Treatment with prolactin for 4 weeks induced a lupus-like phenotype with an increased number of transgene-expressing B cells, elevated serum anti-DNA antibody titers, and glomerular immunoglobulin deposits. Prolactin caused a decrease in the population of transitional B cells and an increase in mature follicular and marginal zone B cells. The DNA-reactive B cells had a follicular cell phenotype. Anti-DNA hybridomas demonstrated that prolactin alters ...
... is a chapter in the book, Rheumatology, containing the following 24 pages: Bone Turnover Biochemical Marker, Antinuclear Antibody, ANA Staining Pattern, Anticentromere Antibody, Anti-Double Stranded DNA Antibody, Anti-Single Stranded DNA Antibody, Antihistone Antibody, Anti-Jo-1 Antibody, Anti-Ku Antibody, Anti-Mi-2 Antibody, Anti-ribosomal P Antibody, Anti-ribonucleoprotein Antibody, Anti-Topoisomerase I Antibody, Anti-Smith Antibody, Anti-Ro Antibody, Anti-La Antibody, Antineutrophil Cytoplasmic Antibody, Antiphospholipid Antibody, Lupus Anticoagulant, Synovial Fluid, Polarized Microscopy, Synovial Fluid White Blood Cell Count, Rheumatoid Factor, Major Histocompatibility Complex Antigens.
Objective. To analyze the clinical value of anti-DFS70 antibodies in a cohort of patients undergoing routine antinuclear antibodies (ANAs) testing. Methods. Sera with a dense fine speckled (DFS) indirect immunofluorescence (IIF) pattern from 100 consecutive patients and 100 patients with other IIF patterns were tested for anti-DFS70 antibodies by a novel chemiluminescence immunoassay (CIA) and for ANA by ANA Screen ELISA (both INOVA). Results. Among the 100 patients with a DFS IIF pattern, 91% were anti-DFS70 positive by CIA compared to 3% in the comparator group . The CIA and IIF titers of anti-DFS antibodies were highly correlated (rho = 0.89). ANA by ELISA was positive in 35% of patients with the DFS IIF pattern as compared to 67% of patients with other patterns . Only 12.0% of patients with DFS pattern and 13.4% with DFS pattern and anti-DFS70 antibodies detected by CIA had systemic autoimmune rheumatic disease (SARD). Only 5/91 (5.5%) patients with anti-DFS70 antibodies had SARD and their sera
Group A only: patients on immunosuppressive treatments had them withdrawn at baseline. All patients were allowed up to 160 mg depomedrol at baseline which could be repeated within two weeks up to a total of 4 shots maximum or until satisfactory improvement. Time to flare was calculated from baseline. moderate disease at baseline was defined as up to 3 BILAG B (moderate disease) organ scores, no BILAG A (severe disease) score and a SLEDAI ,/= 10. Severe disease required ,3 BILAG B, OR at least one BILAG A OR SLEDAI , 10 or meeting criteria for a severe flare on the SELENA SLEDAI flare index. At baseline 25 patients with moderate disease. 16 patients had severe disease. Note: severe rash with A on BILAG is only SLEDAI=2, explaining some discrepancies in measures ...
DESCRIPTION WARNING: Positive ANA Titer: The prolonged administration of procainamide often leads in the development of a positive anti-nuclear antibody (ANA) test, with or without symptoms of a lupus erythematosus-like syndrome. I...
OBJECTIVE: To compare the clinical, laboratory, and demographic variables of women in our clinic with systemic lupus erythematosus (SLE) who have had a pregnancy resulting in a live birth and identify any correlations with either term or preterm delivery. METHODS: Pregnancies in women with SLE from 1999 to 2001 were retrospectively reviewed. We recorded demographic data, disease activity (SLE Disease Activity Index, SLEDAI), obstetric history, prednisone dosage, other medications taken during pregnancy, history of renal disease, and autoantibody status [including antinuclear antibody, anti-DNA, anticardiolipin IgG (aCL), and lupus anticoagulant (LAC)]. Preterm delivery was defined as gestational age at delivery , 37 weeks. We performed a literature survey using PubMed and the key words SLE, pregnancy, and outcome. RESULTS: Of the 72 pregnancies, 28 (38.9%) resulted in preterm deliveries. There were no significant differences in any demographic or disease variables measured comparing term versus ...
Expansion of autoreactive B cells could explain the presence of autoantibodies in the autoimmune disease, systemic lupus erythematosus (SLE). To gain better insight into the production of autoreactive B cells, the authors have developed a drug-induced model of lupus in BALB/c mice transgenic for the heavy chain of an anti-DNA antibody. Normally, the transgenic mice display effective regulation of the transgene-expressing anti-DNA B cells. When treated with exogenous 17-estradiol (E2), autoreactive B cells were activated and mice developed a lupus-like disease. ...
Patients with drug-related lupus erythematosus produce antibodies to nuclear histones which can be detected by a three-step indirect immunofluorescence technique. Procainamide-related antinuclear antibodies were detected by this technique, but hydralazine-related antinuclear antibodies were not. Certain evidence suggests that antibodies induced by the two drugs are reactive with different subclasses of histones. Hydralazine was shown to interact with a soluble DNA-histone complex, and the resulting interaction rendered the histone moiety resistant to trypsin digestion. This mechanism may help to maintain DNA-histone complexes in a potentially immunogenic form and result in the production of autoantibodies ...
Systemic lupus erythematosus (SLE) is a heterogeneous autoimmune disease which may affect multiple organ systems. Interferon alpha (IFNα) and autoantibodies that form immune complexes with nuclear antigens (ANA) are hallmarks believed to drive the disease into a vicious circle of inflammation, tissue damage, autoantigen exposure and autoantibody production.. In SLE, the disease course is characterized by episodes of exacerbations alternating with remissions. In order to best treat the patient it is important to closely monitor symptoms and signs of disease activity. Because of the disease heterogeneity, no single biomarker has yet been found to reflect SLE disease activity in general, although antidouble stranded DNA (anti-dsDNA) antibodies sometimes indicate activity, primarily with renal involvement, and constitutes an item of the SLE disease activity score SLEDAI-2K. However, the method of anti-dsDNA measurement is not standardized and therefore varies between different laboratories. In many ...
GRIEMBERG, Gloria et al. Immunofluorescence assay with Crithidia luciliae for the detection of anti-DNA antibodies: Atypical images and their relationship with Chagas disease and leishmaniasis. Medicina (B. Aires) [online]. 2006, vol.66, n.1, pp. 3-8. ISSN 1669-9106.. Anti-native DNA antibodies can be detected by indirect immunofluorescence assay with Crithidia luciliae, displaying an annular image due to a kinetoplast containing double stranded DNA. Other structures such as membrane, flagellum and basal corpuscle can be stained as well, showing what is called atypical fluorescent images. As C. luciliae belongs to the Trypanosomatidae family, which include the human pathogens Trypanosoma cruzi and Leishmania spp., it was considered that these atypical images could be caused by cross-reactions. Serological studies for Chagas disease were performed in 105 serum samples displaying atypical images. Sixty four percent of the samples from non endemic and 78.3% from endemic areas for Chagas disease ...
Data from a randomized, double-blind, placebo-controlled study in 449 patients of 3 doses of belimumab (1, 4, 10 mg/kg) or placebo plus standard of care therapy (SOC) over a 56-week period were analyzed. The Safety of Estrogens in Lupus Erythematosus: National Assessment (SELENA) version of the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) and British Isles Lupus Assessment Group (BILAG) SLE disease activity instruments, the Short Form 36 health survey, and biomarker analyses were used to create a novel SRI. Response to treatment in a subset of 321 serologically active SLE patients (antinuclear antibodies ≥1:80 and/or anti-double-stranded DNA antibodies ≥30 IU/ml) at baseline was retrospectively evaluated using the SRI. ...
Inflammatory eye disease (uveitis) can develop as a complication in children who have juvenile idiopathic arthritis (JIA). Children and adults who have JIA can develop cataracts, glaucoma, corneal degeneration (band keratopathy), or vision loss.. The incidence of eye disease is 2 to 34 out of 100 children who have JIA.footnote 1 It is most common in oligoarticular and RF-negative polyarticular forms of the disease. Eye disease associated with JIA often has no symptoms, although blurred vision may be an early sign. To prevent eye problems from progressing to the point that vision loss occurs, regular eye examinations by an ophthalmologist are very important for children who have JIA. Eye disease develops in about 30 out of 100 children who have oligoarticular JIA, particularly children who have a positive antinuclear antibody (ANA) test result.footnote 2. Early detection and treatment of inflammatory eye disease gives a child the best chance of a good outcome. Discuss the appropriate examination ...
Definition of mixed connective tissue diseases in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is mixed connective tissue diseases? Meaning of mixed connective tissue diseases as a legal term. What does mixed connective tissue diseases mean in law?
Looking for online definition of mixed connective tissue disease in the Medical Dictionary? mixed connective tissue disease explanation free. What is mixed connective tissue disease? Meaning of mixed connective tissue disease medical term. What does mixed connective tissue disease mean?
70 1 OPTIC NEURITIS, TRANSVERSE MYELITIS, AND ANTI-DNA ANTIBODIES NINE YEARS AFTER THYMECTOMY FOR MYASTHENIA GRAVIS MICHEL GOLDMAN, ALAIN HERODE, SAMUEL BORENSTEIN. and ANDRE ZANEN The occurrence of several autoimmune disorders in the same individual is a frequent observation. An impairment of T suppressor cells could be involved in the pathogenesis of these syndromes, as suggested by the T cell abnormalities that are often encountered in patients with autoimmune diseases. We report the occurrence of optic neuritis and transverse myelitis associated with serologic features of systemic lupus erythematosus (SLE) in a young woman who underwent thymectomy for myasthenia gravis. A particular genetic background and T cell abnormalities could have played a role in the development of this autoimmune syndrome. The problem raised by the diagnosis of SLE in patients with neurologic symptoms and the possible confusion with multiple sclerosis will be emphasized. Case report. A IZyear-old girl was examined in ...
An association between congenital heart block (CHB) and connective tissue diseases such as lupus and Sjögren Syndrome has been reported in the literature [7]. There is a strong correlation between congenital heart block and maternal autoantibodies to 48 kD La, 52 kD Ro and 60 kD Ro ribonucleoproteins. The resultant fibrosis of foetal AV node is thought to be due to immune mediated tissue damage to foetal heart following transplacental passage of maternal IgG autoantibodies [8].. A study by Buyon et al examined 105 mothers with Ro and/or La positive sera, and reported that 113 infants were diagnosed with CHB. Of the 87 pregnancies with adequate records, bradyarrhythmias detected before 30 weeks gestation in 71 (82%) - median 23 weeks. Of the 113 infants, 22 (19%) died, mostly within 3 months of birth. Of the 107 live-born children, 67 (63%) required pacing; 35 within 9 days, 15 within 1 year and 17 after 1 year. Of the 49 mothers who had subsequent pregnancies, 8 (16%) had another child with CHB ...
Title: Mixed Connective Tissue Disease, a Roundabout to Rheumatic Diseases?. VOLUME: 5 ISSUE: 2. Author(s):Silvia Bellando-Randone, Maurizio Cutolo, Laszlo Czirjak and Marco Matucci-Cerinic. Affiliation:Department of Biomedicine, Division of Rheumatology AOUC, DENOThe Center, University of Florence, Villa Monna Tessa, Viale Pieraccini 18, 50139, Firenze, Italy.. Keywords:MCTD, capillaroscopy, therapy, systemic sclerosis. Abstract: Introduction: Mixed Connective Tissue Disease (MCTD) has originally been described as a syndrome that consisted of a combination of features typically found in patients with Systemic lupus erythematosus (SLE), Systemic Sclerosis (SSc), polymyositis/dermatomyositis (PM/DM), or rheumatoid arthritis (RA). Objective: The diagnosis of MCTD still remains controversial and it is critically dependent on the demonstration of high-titer anti-U1-RNP antibodies. When first described, MCTD, was believed to have a good prognosis. After longer follow-up studies this opinion has now ...

Mixed Connective Tissue Disease - MCTD | ARUPConsultMixed Connective Tissue Disease - MCTD | ARUPConsult

Antinuclear Antibody (ANA) with HEp-2 Substrate, IgG by IFA 3000082. Method: Semi-Quantitative Indirect Fluorescent Antibody ... Detects Smith/RNP (ENA) antibody, IgG; Smith (ENA) antibody, IgG; SSA 52 and 60 (Ro) (ENA) antibodies, IgG; SSB (La) (ENA) ... Antinuclear Antibodies. The presence of ANAs is a classic feature of SARDs ; therefore, ANA testing is a useful initial test ... and laboratory testing for antinuclear antibodies (ANAs) as well as antibodies against U1 small nuclear ribonucleoprotein (anti ...
more infohttps://arupconsult.com/content/mixed-connective-tissue-disease

Linear Scleroderma en coup de sabre With Associated Neurologic Abnormalities | Articles | PediatricsLinear Scleroderma en coup de sabre With Associated Neurologic Abnormalities | Articles | Pediatrics

EEG, electroencephalogram • CT, computed tomography • ANA, antinuclear antibodies. REFERENCES. *↵. Peterson LS, Nelson AM, Su ... Her anti-single-stranded DNA level was slightly elevated at 159 μ/ml; her antinuclear antibodies (ANA), rheumatoid factor, ... Laboratory tests including ANA, anticardiolipin antibodies, and anticentromere antibody were normal. In light of her skin ... and anti-single-stranded-DNA antibodies may be present.1,15,16 More specific autoantibodies such as Scl-70, anticentromere, Ro/ ...
more infohttp://pediatrics.aappublications.org/content/117/1/e132.full

Uncategorized - Part 3Uncategorized - Part 3

... and is associated with very high levels of a particular antinuclear antibody called anti-RNP. ... the immune system starts to produce antibodies that fight the infection. T cells are in charge of making the antibodies, and ...
more infohttps://www.stylishmedicalid.com/blog/category/uncategorized/page/3/

What are Antinuclear Antibodies?What are Antinuclear Antibodies?

Antinuclear antibodies (ANA) are autoantibodies directed against the nuclei of some of ones own body cells. They are present ... What are Antinuclear Antibodies?. News-Medical. https://www.news-medical.net/health/What-are-Antinuclear-Antibodies.aspx. ( ... In IF-ANA the bound antibodies are detected using another antibody which binds to human antibody molecules, and which carries a ... In ELISA, the antigen-antibody complexes are detected by another antibody which binds to human antibodies, but which carries an ...
more infohttps://www.news-medical.net/health/What-are-Antinuclear-Antibodies.aspx

Antinuclear Antibody Test | Encyclopedia.comAntinuclear Antibody Test | Encyclopedia.com

Source for information on Antinuclear Antibody Test: Gale Encyclopedia of Medicine, 3rd ed. dictionary. ... Antinuclear Antibody Test Definition The antinuclear antibody (ANA) test is a test done early in the evaluation of a person for ... Antinuclear Antibody Test. Definition. The antinuclear antibody (ANA) test is a test done early in the evaluation of a person ... Autoantibody- An antibody that attacks the bodys own cells or tissues.. Antinuclear antibodies- Autoantibodies that attack ...
more infohttps://www.encyclopedia.com/medicine/encyclopedias-almanacs-transcripts-and-maps/antinuclear-antibody-test

Antinuclear antibody panel: MedlinePlus Medical EncyclopediaAntinuclear antibody panel: MedlinePlus Medical Encyclopedia

The antinuclear antibody panel is a blood test that looks at antinuclear antibodies (ANA). ... Antinuclear antibodies (ANA). www.rheumatology.org/I-Am-A/Patient-Caregiver/Diseases-Conditions/Antinuclear-Antibodies-ANA. ... The antinuclear antibody panel is a blood test that looks at antinuclear antibodies (ANA). ... ANA are antibodies produced by the immune system that bind to the bodys own tissues. The antinuclear antibody test looks for ...
more infohttps://medlineplus.gov/ency/article/003535.htm

Antinuclear antibodies and respon... preview & related info | MendeleyAntinuclear antibodies and respon... preview & related info | Mendeley

According to the presence of antinuclear antibodies (ANA) at baseline and during the f... ... Antinuclear antibodies and response to IFNbeta-1a therapy in relapsing-remitting multiple sclerosis.. *Ciccarelli O ... According to the presence of antinuclear antibodies (ANA) at baseline and during the first 6 months of treatment, patients were ...
more infohttps://www.mendeley.com/research-papers/antinuclear-antibodies-response-ifnbeta1a-therapy-relapsingremitting-multiple-sclerosis/

Antinuclear Antibody Panel: Purpose, Results, and RisksAntinuclear Antibody Panel: Purpose, Results, and Risks

What is an antinuclear antibody panel?. Antibodies are proteins made by your immune system. They help your body recognize and ... When is an antinuclear antibody panel needed?. Your doctor will likely order an ANA panel if you have signs or symptoms of an ... Antinuclear Antibody Panel (ANA Test). Medically reviewed by Debra Sullivan, PhD, MSN, RN, CNE, COI on August 30, 2017. - ... Antibodies that attack healthy proteins within the nucleus - the control center of your cells - are called antinuclear ...
more infohttps://www.healthline.com/health/antinuclear-antibody-panel

Antinuclear Antibodies (ANA)Antinuclear Antibodies (ANA)

... Test Overview. An antinuclear antibody (ANA) test measures the amount and pattern of antibodies ... An antinuclear antibody (ANA) test measures the amount and pattern of antibodies in your blood that work against your own body ... An antinuclear antibodies (ANA) test is done to help identify problems with the immune system, such as:. *Rheumatoid arthritis. ... When a person has an autoimmune disease, the immune system produces antibodies that attach to the bodys own cells as though ...
more infohttps://www.lmh.org/wellness/health-library/document-viewer/?id=hw2297

164920: Antinuclear Antibodies (ANA) Profile, 11... | LabCorp164920: Antinuclear Antibodies (ANA) Profile, 11... | LabCorp

Antinuclear antibodies: An overview. In: Wallace DJ, Hahn BH, eds. Dubois Lupus Erythematosus. 5th ed. Baltimore, Md: Williams ... Antinuclear Antibodies (ANA) Profile, 11 biomarkers, by Multiplex Immunoassay, dsDNA, RNP, Sm, SS-A, SS-B, Scl-70, Chromatin, ... Antichromatin Antibodies. AI. 51775-5. 164920. ANA Comprehensive Plus Profile. 012701. Antiribosomal P Antibodies. AI. 13636-6 ... Detection of antinuclear antibodies: Comparative evaluation of enzyme immunoassay and indirect immunofluorescence methods. Arch ...
more infohttps://www.labcorp.com/test-menu/20226/antinuclear-antibodies-ana-profile-11-biomarkers-by-multiplex-immunoassay-dsdna-rnp-sm-ss-a-ss-b-scl-70-chromatin-jo-1-centromere-b-sm-rnp-ribosomal-p

The Prevalence of Antinuclear Antibodies in Patients with SarcoidosisThe Prevalence of Antinuclear Antibodies in Patients with Sarcoidosis

Antinuclear antibodies are the markers used for connective tissue diseases. Aim. To determine antinuclear antibody frequency ... Antinuclear antibody (ANA) is the brand name of the antibodies towards nuclear and cytoplasmic structures of the cell. ANA is a ... The Prevalence of Antinuclear Antibodies in Patients with Sarcoidosis. Senol Kobak,1 Hatice Yilmaz,1 Fidan Sever,2 Arzu Duran,3 ... anti-Ro antibody in one patient, anti-Scl-70 antibody in one patient, and anti-dsDNA antibody in one patient, and eight ...
more infohttps://www.hindawi.com/journals/ad/2014/351852/

Antinuclear Antibodies (ANA) | Health Testing CentersAntinuclear Antibodies (ANA) | Health Testing Centers

Sometimes antibodies are made in response to healthy cells. Antinuclear antibodies are antibodies made against antigens ( ... This test measures the level of antinuclear antibodies. Antibodies are made by the body to fight infection or other "foreign" ... Sample Result: Antinuclear Antibodies (ANA). This browser does not support inline PDFs. To view the Sample Results file, click ... T3 Total and Thyroid Peroxidase Antibody (TPO). ...
more infohttps://www.healthtestingcenters.com/test/antinuclear-antibodies-ana-0/

Antinuclear Antibodies (ANA) - WellSpan Health LibraryAntinuclear Antibodies (ANA) - WellSpan Health Library

... test measures the amount and pattern of antibodies in your blood that work against your own body (autoimmune reaction). The ... An antinuclear antibody (ANA) test measures the amount and pattern of antibodies in your blood that work against your own body ... An antinuclear antibody (ANA) test measures the amount and pattern of antibodies in your blood that work against your own body ... An antinuclear antibodies (ANA) test is done to help identify problems with the immune system, such as:. *Rheumatoid arthritis. ...
more infohttps://www.wellspan.org/health-library/Document.aspx?id=hw2297

What Doctors Dont Tell You: Antinuclear antibody testsWhat Doctors Don't Tell You: Antinuclear antibody tests

... he may arrange to have you take an antinuclear antibody (ANA) test. ... he may arrange to have you take an antinuclear antibody (ANA) test. In these kinds of connective-tissue diseases, the immune- ... So, while a positive result for double-stranded DNA and SM antibodies may confirm SLE, a negative test doesnt necessarily rule ... Healthy people can also show a positive result: around 2 per cent of the population have mildly elevated antibodies without ...
more infohttp://www.healthy.net/Health/Article/Antinuclear_antibody_tests/3651

Wiley: Accurate Interpretation of Antinuclear Antibodies Test Key to Confirming Autoimmune DiseaseWiley: Accurate Interpretation of Antinuclear Antibodies Test Key to Confirming Autoimmune Disease

Accurate Interpretation of Antinuclear Antibodies Test Key to Confirming Autoimmune Disease. New Research Finds Pattern for ... The presence of antinuclear antibodies (ANA) indicates the possibility of autoimmunity and the indirect immunofluorescence (IIF ...
more infohttps://www.wiley.com/WileyCDA/PressRelease/pressReleaseId-88698.html

Antinuclear antibodies | definition of Antinuclear antibodies by Medical dictionaryAntinuclear antibodies | definition of Antinuclear antibodies by Medical dictionary

What is Antinuclear antibodies? Meaning of Antinuclear antibodies medical term. What does Antinuclear antibodies mean? ... Looking for online definition of Antinuclear antibodies in the Medical Dictionary? Antinuclear antibodies explanation free. ... Related to Antinuclear antibodies: Lupus. Antinuclear antibodies. Autoantibodies that attack substances found in the center, or ... Antinuclear antibodies (ANA) and anti-double stranded DNA (dsDNA) antibodies are important antibodies determinants in the ...
more infohttp://medical-dictionary.thefreedictionary.com/Antinuclear+antibodies

ANA (Antinuclear Antibody) Test: MedlinePlus Lab Test InformationANA (Antinuclear Antibody) Test: MedlinePlus Lab Test Information

An antinuclear antibody test checks to see if you have an autoimmune disorder, a condition where the immune system attacks ... antinuclear antibody) test?. An ANA test looks for antinuclear antibodies in your blood. If the test finds antinuclear ... medlineplus.gov/lab-tests/ana-antinuclear-antibody-test/ ANA (Antinuclear Antibody) Test. ... But an antinuclear antibody attacks your own healthy cells instead. Its called "antinuclear" because it targets the nucleus ( ...
more infohttps://medlineplus.gov/lab-tests/ana-antinuclear-antibody-test/

Nitrofurantoin-induced antinuclear antibodies and panniculitis.Nitrofurantoin-induced antinuclear antibodies and panniculitis.

... код для вставки. код для вставки на сайт или в блог. Ширина: ( ... Arthritis Rheum 20: 1121-1 124, 1977 Nitrofurantoin-induced antinuclear antibodies and panniculitis To the Editor: The causes ... a patient who had nitrofurantoin-induced panniculitis with associated serologic abnormalities of positive antinuclear antibody ... Circulating antibody to C dificile toxin was demonstrated in that patient, whereas 11 other patients with PMC-positive fecal ...
more infohttps://www.docme.ru/doc/2169604/nitrofurantoin-induced-antinuclear-antibodies-and-pannicu..

Understanding antinuclear antibodies | The Journal of the American Osteopathic AssociationUnderstanding antinuclear antibodies | The Journal of the American Osteopathic Association

Understanding antinuclear antibodies You will receive an email whenever this article is corrected, updated, or cited in the ... Understanding antinuclear antibodies. The Journal of the American Osteopathic Association, June 1991, Vol. 91, 563. doi:10.7556 ... Pertusi R, Rubin B. Understanding antinuclear antibodies. J Am Osteopath Assoc 1991;91(6):563. doi: 10.7556/jaoa.1991.91.6.563. ... diagnostic criteria for connective tissue disorders frequently include positive antinuclear antibody (ANA) assays. Proper ...
more infohttps://jaoa.org/article.aspx?articleid=2098688

Benign Versus Malignant Antinuclear Antibodies. | Annals of Internal Medicine | American College of Physicians"Benign" Versus "Malignant" Antinuclear Antibodies. | Annals of Internal Medicine | American College of Physicians

A Novel Antinuclear Antibody Associated with a Lupus-like Paraneoplastic Syndrome Annals of Internal Medicine; 109 (4): 295-297 ... Isoniazid Induction of Antinuclear Antibodies: A Prospective Study Annals of Internal Medicine; 88 (5): 650-652 ... Profiles of Antinuclear Antibodies in Systemic Rheumatic Diseases Annals of Internal Medicine; 83 (4): 464-469 ... "Benign" Versus "Malignant" Antinuclear Antibodies. George J. Friou, M.D., F.A.C.P.; Takeshi Tojo, M.D. ...
more infohttp://annals.org/aim/article-abstract/683283/benign-versus-malignant-antinuclear-antibodies

PDF] Antinuclear antibody test. | Semantic ScholarPDF] Antinuclear antibody test. | Semantic Scholar

Antinuclear antibodies are immunoglobulins or antibodies that bind to one or more antigens expressed within the nucleus of ... The antinuclear antibody (ANA) test is widely used as a serological marker of autoimmune disease. ... The antinuclear antibody (ANA) test is widely used as a serological marker of autoimmune disease. Antinuclear antibodies are ... Antinuclear antibody test.. @article{OSullivan2013AntinuclearAT, title={Antinuclear antibody test.}, author={Michael OSullivan ...
more infohttps://www.semanticscholar.org/paper/Antinuclear-antibody-test.-O%27Sullivan-Mclean-Tooke/9d9847b68dda073207c8d4c989c15f842686bdf2

Detection of antinuclear antibodies by a colloidal gold modified optical fiber: comparison with ELISA | SpringerLinkDetection of antinuclear antibodies by a colloidal gold modified optical fiber: comparison with ELISA | SpringerLink

A fiberoptic evanescent-wave sensor has been developed for the measurement of antinuclear antibodies in sera from patients and ... Antinuclear antibodies Fiberoptic biosensor Localized surface plasmon resonance Gold nanoparticles Enzyme-linked immunosorbent ... Detection of antinuclear antibodies by a colloidal gold modified optical fiber: comparison with ELISA. ... A fiberoptic evanescent-wave sensor has been developed for the measurement of antinuclear antibodies in sera from patients and ...
more infohttps://link.springer.com/article/10.1007%2Fs00216-007-1276-1

Antinuclear antibody | Article about antinuclear antibody by The Free DictionaryAntinuclear antibody | Article about antinuclear antibody by The Free Dictionary

Find out information about antinuclear antibody. Antibody to deoxyribonucleic acid, ribonucleic acid, histone, or nonhistone ... proteins found in the serum of individuals with certain autoimmune diseases.... Explanation of antinuclear antibody ... Antinuclear antibody, anti-nRNP antibody and anti-histone antibody were positive; however, anti-dsDNA antibody, anti-sm ... antinuclear antibody. Also found in: Dictionary, Thesaurus, Medical, Acronyms. antinuclear antibody. [¦an·tē¦nü·klē·ər ′an·tə‚ ...
more infohttps://encyclopedia2.thefreedictionary.com/antinuclear+antibody

Anti-nuclear antibody? | DailyStrengthAnti-nuclear antibody? | DailyStrength

Anti-nuclear antibody?. deleted_user 09/26/2009. Okay my blood work has not been explained by a doctor yet, but I have a copy ...
more infohttps://www.dailystrength.org/group/ankylosing-spondylitis/discussion/anti-nuclear-antibody
  • We wanted to keep ANA IFA as our principle screening method, possibly reserving multiplex or ELISA methods to determine specific antibodies-a decision with which our physicians concurred. (aacc.org)
  • It certainly detects more antibodies than any other method, but ELISA and multiplex techniques specifically identify which antibody a patient has as opposed to providing a pattern in the IFA procedure. (aacc.org)