Unique genetically-controlled determinants present on ANTIBODIES whose specificity is limited to a single group of proteins (e.g., another antibody molecule or an individual myeloma protein). The idiotype appears to represent the antigenicity of the antigen-binding site of the antibody and to be genetically codetermined with it. The idiotypic determinants have been precisely located to the IMMUNOGLOBULIN VARIABLE REGION of both immunoglobin polypeptide chains.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Antibodies which react with the individual structural determinants (idiotopes) on the variable region of other antibodies.
Antibodies produced by a single clone of cells.
Immunoglobulins produced in response to VIRAL ANTIGENS.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.
Local surface sites on antibodies which react with antigen determinant sites on antigens (EPITOPES.) They are formed from parts of the variable regions of FAB FRAGMENTS.
Sites on an antigen that interact with specific antibodies.
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
Antibodies that reduce or abolish some biological activity of a soluble antigen or infectious agent, usually a virus.
The processes triggered by interactions of ANTIBODIES with their ANTIGENS.
A hapten capable of eliciting both antibody formation and delayed hypersensitivity when bound to aromatic amino acids, polypeptides or proteins. It is used as an immunologic research tool.
A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.
Immunoglobulins induced by antigens specific for tumors other than the normally occurring HISTOCOMPATIBILITY ANTIGENS.
Abnormal immunoglobulins characteristic of MULTIPLE MYELOMA.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes.
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
That region of the immunoglobulin molecule that varies in its amino acid sequence and composition, and comprises the binding site for a specific antigen. It is located at the N-terminus of the Fab fragment of the immunoglobulin. It includes hypervariable regions (COMPLEMENTARITY DETERMINING REGIONS) and framework regions.
Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell.
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
Small antigenic determinants capable of eliciting an immune response only when coupled to a carrier. Haptens bind to antibodies but by themselves cannot elicit an antibody response.
Antibodies that react with self-antigens (AUTOANTIGENS) of the organism that produced them.
Autoantibodies directed against various nuclear antigens including DNA, RNA, histones, acidic nuclear proteins, or complexes of these molecular elements. Antinuclear antibodies are found in systemic autoimmune diseases including systemic lupus erythematosus, Sjogren's syndrome, scleroderma, polymyositis, and mixed connective tissue disease.
Univalent antigen-binding fragments composed of one entire IMMUNOGLOBULIN LIGHT CHAIN and the amino terminal end of one of the IMMUNOGLOBULIN HEAVY CHAINS from the hinge region, linked to each other by disulfide bonds. Fab contains the IMMUNOGLOBULIN VARIABLE REGIONS, which are part of the antigen-binding site, and the first IMMUNOGLOBULIN CONSTANT REGIONS. This fragment can be obtained by digestion of immunoglobulins with the proteolytic enzyme PAPAIN.
Stable iodine atoms that have the same atomic number as the element iodine, but differ in atomic weight. I-127 is the only naturally occurring stable iodine isotope.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Antibodies reactive with HIV ANTIGENS.
The complex formed by the binding of antigen and antibody molecules. The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES.
Immunoglobulins produced in a response to PROTOZOAN ANTIGENS.
Allelic variants of the immunoglobulin light chains (IMMUNOGLOBULIN LIGHT CHAINS) or heavy chains (IMMUNOGLOBULIN HEAVY CHAINS) encoded by ALLELES of IMMUNOGLOBULIN GENES.
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
The largest of polypeptide chains comprising immunoglobulins. They contain 450 to 600 amino acid residues per chain, and have molecular weights of 51-72 kDa.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Partial immunoglobulin molecules resulting from selective cleavage by proteolytic enzymes or generated through PROTEIN ENGINEERING techniques.
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
One of the types of light chains of the immunoglobulins with a molecular weight of approximately 22 kDa.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
The phenomenon of immense variability characteristic of ANTIBODIES. It enables the IMMUNE SYSTEM to react specifically against the essentially unlimited kinds of ANTIGENS it encounters. Antibody diversity is accounted for by three main theories: (1) the Germ Line Theory, which holds that each antibody-producing cell has genes coding for all possible antibody specificities, but expresses only the one stimulated by antigen; (2) the Somatic Mutation Theory, which holds that antibody-producing cells contain only a few genes, which produce antibody diversity by mutation; and (3) the Gene Rearrangement Theory, which holds that antibody diversity is generated by the rearrangement of IMMUNOGLOBULIN VARIABLE REGION gene segments during the differentiation of the ANTIBODY-PRODUCING CELLS.
Polypeptide chains, consisting of 211 to 217 amino acid residues and having a molecular weight of approximately 22 kDa. There are two major types of light chains, kappa and lambda. Two Ig light chains and two Ig heavy chains (IMMUNOGLOBULIN HEAVY CHAINS) make one immunoglobulin molecule.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed)
Transfer of immunity from immunized to non-immune host by administration of serum antibodies, or transplantation of lymphocytes (ADOPTIVE TRANSFER).
Represents 15-20% of the human serum immunoglobulins, mostly as the 4-chain polymer in humans or dimer in other mammals. Secretory IgA (IMMUNOGLOBULIN A, SECRETORY) is the main immunoglobulin in secretions.
Immunoglobulins produced in a response to FUNGAL ANTIGENS.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
A subdiscipline of genetics which deals with the genetic basis of the immune response (IMMUNITY).
Substances that are recognized by the immune system and induce an immune reaction.
The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).
Calcium and magnesium salts used therapeutically in hepatobiliary dysfunction.
Serologic tests in which a known quantity of antigen is added to the serum prior to the addition of a red cell suspension. Reaction result is expressed as the smallest amount of antigen which causes complete inhibition of hemagglutination.
Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.
Multi-subunit proteins which function in IMMUNITY. They are produced by B LYMPHOCYTES from the IMMUNOGLOBULIN GENES. They are comprised of two heavy (IMMUNOGLOBULIN HEAVY CHAINS) and two light chains (IMMUNOGLOBULIN LIGHT CHAINS) with additional ancillary polypeptide chains depending on their isoforms. The variety of isoforms include monomeric or polymeric forms, and transmembrane forms (B-CELL ANTIGEN RECEPTORS) or secreted forms (ANTIBODIES). They are divided by the amino acid sequence of their heavy chains into five classes (IMMUNOGLOBULIN A; IMMUNOGLOBULIN D; IMMUNOGLOBULIN E; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M) and various subclasses.
Antibodies, often monoclonal, in which the two antigen-binding sites are specific for separate ANTIGENIC DETERMINANTS. They are artificial antibodies produced by chemical crosslinking, fusion of HYBRIDOMA cells, or by molecular genetic techniques. They function as the main mediators of targeted cellular cytotoxicity and have been shown to be efficient in the targeting of drugs, toxins, radiolabeled haptens, and effector cells to diseased tissue, primarily tumors.
Any discrete, presumably solitary, mass of neoplastic PLASMA CELLS either in BONE MARROW or various extramedullary sites.
Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.
One of the types of light chain subunits of the immunoglobulins with a molecular weight of approximately 22 kDa.
A form of antibodies consisting only of the variable regions of the heavy and light chains (FV FRAGMENTS), connected by a small linker peptide. They are less immunogenic than complete immunoglobulin and thus have potential therapeutic use.
Antibodies that inhibit the reaction between ANTIGEN and other antibodies or sensitized T-LYMPHOCYTES (e.g., antibodies of the IMMUNOGLOBULIN G class that compete with IGE antibodies for antigen, thereby blocking an allergic response). Blocking antibodies that bind tumors and prevent destruction of tumor cells by CYTOTOXIC T-LYMPHOCYTES have also been called enhancing antibodies. (Rosen et al., Dictionary of Immunology, 1989)
A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
Antibodies elicited in a different species from which the antigen originated. These antibodies are directed against a wide variety of interspecies-specific antigens, the best known of which are Forssman, Hanganutziu-Deicher (H-D), and Paul-Bunnell (P-B). Incidence of antibodies to these antigens--i.e., the phenomenon of heterophile antibody response--is useful in the serodiagnosis, pathogenesis, and prognosis of infection and latent infectious states as well as in cancer classification.
Cells of the lymphoid series that can react with antigen to produce specific cell products called antibodies. Various cell subpopulations, often B-lymphocytes, can be defined, based on the different classes of immunoglobulins that they synthesize.
Antibodies that can catalyze a wide variety of chemical reactions. They are characterized by high substrate specificity and share many mechanistic features with enzymes.
Serum globulins with high molecular weight. (Dorland, 28th ed)
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Antibodies from an individual that react with ISOANTIGENS of another individual of the same species.
Established cell cultures that have the potential to propagate indefinitely.
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
Polysaccharides found in bacteria and in capsules thereof.
An encapsulated lymphatic organ through which venous blood filters.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
A specific immune response elicited by a specific dose of an immunologically active substance or cell in an organism, tissue, or cell.
The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.
Antibodies from non-human species whose protein sequences have been modified to make them nearly identical with human antibodies. If the constant region and part of the variable region are replaced, they are called humanized. If only the constant region is modified they are called chimeric. INN names for humanized antibodies end in -zumab.
A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
Derivatives of phenylacetic acid. Included under this heading are a variety of acid forms, salts, esters, and amides that contain the benzeneacetic acid structure. Note that this class of compounds should not be confused with derivatives of phenyl acetate, which contain the PHENOL ester of ACETIC ACID.
Abnormal immunoglobulins, especially IGG or IGM, that precipitate spontaneously when SERUM is cooled below 37 degrees Celsius. It is characteristic of CRYOGLOBULINEMIA.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Methods used for studying the interactions of antibodies with specific regions of protein antigens. Important applications of epitope mapping are found within the area of immunochemistry.
Unstable isotopes of iodine that decay or disintegrate emitting radiation. I atoms with atomic weights 117-139, except I 127, are radioactive iodine isotopes.
Autoantibodies directed against phospholipids. These antibodies are characteristically found in patients with systemic lupus erythematosus (LUPUS ERYTHEMATOSUS, SYSTEMIC;), ANTIPHOSPHOLIPID SYNDROME; related autoimmune diseases, some non-autoimmune diseases, and also in healthy individuals.
Antibodies found in adult RHEUMATOID ARTHRITIS patients that are directed against GAMMA-CHAIN IMMUNOGLOBULINS.
A chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow.
Any of numerous agile, hollow-horned RUMINANTS of the genus Capra, in the family Bovidae, closely related to the SHEEP.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
Abnormal immunoglobulins synthesized by atypical cells of the MONONUCLEAR PHAGOCYTE SYSTEM. Paraproteins containing only light chains lead to Bence Jones paraproteinemia, while the presence of only atypical heavy chains leads to heavy chain disease. Most of the paraproteins show themselves as an M-component (monoclonal gammopathy) in electrophoresis. Diclonal and polyclonal paraproteins are much less frequently encountered.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Proteins prepared by recombinant DNA technology.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
A method to identify and enumerate cells that are synthesizing ANTIBODIES against ANTIGENS or HAPTENS conjugated to sheep RED BLOOD CELLS. The sheep red blood cells surrounding cells secreting antibody are lysed by added COMPLEMENT producing a clear zone of HEMOLYSIS. (From Illustrated Dictionary of Immunology, 3rd ed)
Disorders that are characterized by the production of antibodies that react with host tissues or immune effector cells that are autoreactive to endogenous peptides.
Deliberate prevention or diminution of the host's immune response. It may be nonspecific as in the administration of immunosuppressive agents (drugs or radiation) or by lymphocyte depletion or may be specific as in desensitization or the simultaneous administration of antigen and immunosuppressive drugs.
IMMUNOGLOBULINS on the surface of B-LYMPHOCYTES. Their MESSENGER RNA contains an EXON with a membrane spanning sequence, producing immunoglobulins in the form of type I transmembrane proteins as opposed to secreted immunoglobulins (ANTIBODIES) which do not contain the membrane spanning segment.
Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.
Substances elaborated by bacteria that have antigenic activity.
A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Organic compounds that contain two nitro groups attached to a phenol.
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
Administration of vaccines to stimulate the host's immune response. This includes any preparation intended for active immunological prophylaxis.
The sum of the weight of all the atoms in a molecule.
Elements of limited time intervals, contributing to particular results or situations.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
Endogenous tissue constituents that have the ability to interact with AUTOANTIBODIES and cause an immune response.
Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
A group of antigens that includes both the major and minor histocompatibility antigens. The former are genetically determined by the major histocompatibility complex. They determine tissue type for transplantation and cause allograft rejections. The latter are systems of allelic alloantigens that can cause weak transplant rejection.
Antibodies to the HEPATITIS C ANTIGENS including antibodies to envelope, core, and non-structural proteins.
A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.
A plant family of the order Geraniales, subclass Rosidae, class Magnoliopsida.
Substances elaborated by viruses that have antigenic activity.
An immunoglobulin which accounts for less than 1% of plasma immunoglobulin. It is found on the membrane of many circulating B LYMPHOCYTES.
Leukemia associated with HYPERPLASIA of the lymphoid tissues and increased numbers of circulating malignant LYMPHOCYTES and lymphoblasts.
T-cell enhancement of the B-cell response to thymic-dependent antigens.
Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
A genus of gram-positive, spherical bacteria found in soils and fresh water, and frequently on the skin of man and other animals.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Manifestations of the immune response which are mediated by antigen-sensitized T-lymphocytes via lymphokines or direct cytotoxicity. This takes place in the absence of circulating antibody or where antibody plays a subordinate role.
Autoantibodies directed against cytoplasmic constituents of POLYMORPHONUCLEAR LEUKOCYTES and/or MONOCYTES. They are used as specific markers for GRANULOMATOSIS WITH POLYANGIITIS and other diseases, though their pathophysiological role is not clear. ANCA are routinely detected by indirect immunofluorescence with three different patterns: c-ANCA (cytoplasmic), p-ANCA (perinuclear), and atypical ANCA.
The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
EPIDEMIOLOGIC STUDIES based on the detection through serological testing of characteristic change in the serum level of specific ANTIBODIES. Latent subclinical infections and carrier states can thus be detected in addition to clinically overt cases.
Techniques used to demonstrate or measure an immune response, and to identify or measure antigens using antibodies.
A group of glucose polymers made by certain bacteria. Dextrans are used therapeutically as plasma volume expanders and anticoagulants. They are also commonly used in biological experimentation and in industry for a wide variety of purposes.
Techniques for removal by adsorption and subsequent elution of a specific antibody or antigen using an immunosorbent containing the homologous antigen or antibody.
The rate dynamics in chemical or physical systems.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
A collection of cloned peptides, or chemically synthesized peptides, frequently consisting of all possible combinations of amino acids making up an n-amino acid peptide.
Vaccines or candidate vaccines designed to prevent or treat cancer. Vaccines are produced using the patient's own whole tumor cells as the source of antigens, or using tumor-specific antigens, often recombinantly produced.
The classes of immunoglobulins found in any species of animal. In man there are nine classes that migrate in five different groups in electrophoresis; they each consist of two light and two heavy protein chains, and each group has distinguishing structural and functional properties.
A group of heterogeneous lymphoid tumors generally expressing one or more B-cell antigens or representing malignant transformations of B-lymphocytes.
Proteins, protein complexes, or glycoproteins secreted by suppressor T-cells that inhibit either subsequent T-cells, B-cells, or other immunologic phenomena. Some of these factors have both histocompatibility (I-J) and antigen-specific domains which may be linked by disulfide bridges. They can be elicited by haptens or other antigens and may be mass-produced by hybridomas or monoclones in the laboratory.
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
The genetic region which contains the loci of genes which determine the structure of the serologically defined (SD) and lymphocyte-defined (LD) TRANSPLANTATION ANTIGENS, genes which control the structure of the IMMUNE RESPONSE-ASSOCIATED ANTIGENS, HUMAN; the IMMUNE RESPONSE GENES which control the ability of an animal to respond immunologically to antigenic stimuli, and genes which determine the structure and/or level of the first four components of complement.
Antibodies obtained from a single clone of cells grown in mice or rats.
Any cell, other than a ZYGOTE, that contains elements (such as NUCLEI and CYTOPLASM) from two or more different cells, usually produced by artificial CELL FUSION.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
Antigens that exist in alternative (allelic) forms in a single species. When an isoantigen is encountered by species members who lack it, an immune response is induced. Typical isoantigens are the BLOOD GROUP ANTIGENS.
A basic constituent of lecithin that is found in many plants and animal organs. It is important as a precursor of acetylcholine, as a methyl donor in various metabolic processes, and in lipid metabolism.
Antibodies to the HEPATITIS B ANTIGENS, including antibodies to the surface (Australia) and core of the Dane particle and those to the "e" antigens.
A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.
The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.
Resistance to a disease-causing agent induced by the introduction of maternal immunity into the fetus by transplacental transfer or into the neonate through colostrum and milk.
Genes encoding the different subunits of the IMMUNOGLOBULINS, for example the IMMUNOGLOBULIN LIGHT CHAIN GENES and the IMMUNOGLOBULIN HEAVY CHAIN GENES. The heavy and light immunoglobulin genes are present as gene segments in the germline cells. The completed genes are created when the segments are shuffled and assembled (B-LYMPHOCYTE GENE REARRANGEMENT) during B-LYMPHOCYTE maturation. The gene segments of the human light and heavy chain germline genes are symbolized V (variable), J (joining) and C (constant). The heavy chain germline genes have an additional segment D (diversity).
Antibodies specific to INSULIN.
An immunological attack mounted by a graft against the host because of tissue incompatibility when immunologically competent cells are transplanted to an immunologically incompetent host; the resulting clinical picture is that of GRAFT VS HOST DISEASE.
Serum glycoproteins participating in the host defense mechanism of COMPLEMENT ACTIVATION that creates the COMPLEMENT MEMBRANE ATTACK COMPLEX. Included are glycoproteins in the various pathways of complement activation (CLASSICAL COMPLEMENT PATHWAY; ALTERNATIVE COMPLEMENT PATHWAY; and LECTIN COMPLEMENT PATHWAY).
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
The phenomenon of target cell destruction by immunologically active effector cells. It may be brought about directly by sensitized T-lymphocytes or by lymphoid or myeloid "killer" cells, or it may be mediated by cytotoxic antibody, cytotoxic factor released by lymphoid cells, or complement.
The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.
The characteristic three-dimensional shape of a molecule.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
A malignancy of mature PLASMA CELLS engaging in monoclonal immunoglobulin production. It is characterized by hyperglobulinemia, excess Bence-Jones proteins (free monoclonal IMMUNOGLOBULIN LIGHT CHAINS) in the urine, skeletal destruction, bone pain, and fractures. Other features include ANEMIA; HYPERCALCEMIA; and RENAL INSUFFICIENCY.
Any part or derivative of any protozoan that elicits immunity; malaria (Plasmodium) and trypanosome antigens are presently the most frequently encountered.
Derivatives of BENZOIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxybenzene structure.
Diagnostic procedures involving immunoglobulin reactions.
The phenomenon of antibody-mediated target cell destruction by non-sensitized effector cells. The identity of the target cell varies, but it must possess surface IMMUNOGLOBULIN G whose Fc portion is intact. The effector cell is a "killer" cell possessing Fc receptors. It may be a lymphocyte lacking conventional B- or T-cell markers, or a monocyte, macrophage, or polynuclear leukocyte, depending on the identity of the target cell. The reaction is complement-independent.
A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.
The major group of transplantation antigens in the mouse.
An immunoglobulin fragment composed of one variable domain from an IMMUNOGLOBULIN HEAVY CHAIN or IMMUNOGLOBULIN LIGHT CHAIN.
Manipulation of the host's immune system in treatment of disease. It includes both active and passive immunization as well as immunosuppressive therapy to prevent graft rejection.
The type species of LENTIVIRUS and the etiologic agent of AIDS. It is characterized by its cytopathic effect and affinity for the T4-lymphocyte.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Genetic loci in the vertebrate major histocompatibility complex that encode polymorphic products which control the immune response to specific antigens. The genes are found in the HLA-D region in humans and in the I region in mice.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Suspensions of attenuated or killed bacteria administered for the prevention or treatment of infectious bacterial disease.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Field of chemistry that pertains to immunological phenomena and the study of chemical reactions related to antigen stimulation of tissues. It includes physicochemical interactions between antigens and antibodies.
Layers of protein which surround the capsid in animal viruses with tubular nucleocapsids. The envelope consists of an inner layer of lipids and virus specified proteins also called membrane or matrix proteins. The outer layer consists of one or more types of morphological subunits called peplomers which project from the viral envelope; this layer always consists of glycoproteins.
Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios.
A genus of gram-positive, coccoid bacteria whose organisms occur in pairs or chains. No endospores are produced. Many species exist as commensals or parasites on man or animals with some being highly pathogenic. A few species are saprophytes and occur in the natural environment.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
The altered state of immunologic responsiveness resulting from initial contact with antigen, which enables the individual to produce antibodies more rapidly and in greater quantity in response to secondary antigenic stimulus.
A cell line derived from cultured tumor cells.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
Glycoproteins found on the membrane or surface of cells.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Inorganic or organic compounds that contain arsenic.
Radiotherapy where cytotoxic radionuclides are linked to antibodies in order to deliver toxins directly to tumor targets. Therapy with targeted radiation rather than antibody-targeted toxins (IMMUNOTOXINS) has the advantage that adjacent tumor cells, which lack the appropriate antigenic determinants, can be destroyed by radiation cross-fire. Radioimmunotherapy is sometimes called targeted radiotherapy, but this latter term can also refer to radionuclides linked to non-immune molecules (see RADIOTHERAPY).
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.

Prevention of collagen-induced arthritis by gene delivery of soluble p75 tumour necrosis factor receptor. (1/2060)

Collagen type II-induced arthritis (CIA) in DBA/1 mice can be passively transferred to SCID mice with spleen B- and T-lymphocytes. In the present study, we show that infection ex vivo of splenocytes from arthritic DBA/1 mice with a retroviral vector, containing cDNA for the soluble form of human p75 receptor of tumour necrosis factor (TNF-R) before transfer, prevents the development of arthritis, bone erosion and joint inflammation in the SCID recipients. Assessment of IgG subclass levels and studies of synovial histology suggest that down-regulating the effector functions of T helper-type 1 (Th1) cells may, at least in part, explain the inhibition of arthritis in the SCID recipients. In contrast, the transfer of splenocytes infected with mouse TNF-alpha gene construct resulted in exacerbated arthritis and enhancement of IgG2a antibody levels. Intriguingly, infection of splenocytes from arthritic DBA/1 mice with a construct for mouse IL-10 had no modulating effect on the transfer of arthritis. The data suggest that manipulation of the immune system with cytokines, or cytokine inhibitors using gene transfer protocols can be an effective approach to ameliorate arthritis.  (+info)

Human triclonal anti-IgG gammopathy. I. Iso-electric focusing characteristics of the IgG, IgA and IgM anti-IgG and their heavy and light chains. (2/2060)

Human IgG, IgA and IgM anti-IgG autoantibodies have been isolated from the serum of an individual with Felty's syndrome. These were initially noted as soluble circulating serum complexes by analytical ultracentrifugation. Isolation was accomplished by solid phase immunoadsorption and each of the three antibody populations obtained was shown to be of restricted heterogeneity by liquid and polyacrylamide gel electrofocussing methods. Type kappa light chains were obtained from each protein. Co-isoelectric focusing experiments of all possible pairs of these light chains showed them to have identical net charge characteristics. Heavy chains obtained from each protein were also monoclonal and of differing isoelectric point. The availability of this serum provides a human model with which to study the changes which may occur in autoantibodies during the autoimmune response.  (+info)

Plasma anti-serotonin and serotonin anti-idiotypic antibodies are elevated in panic disorder. (3/2060)

The psychoneuroimmunology of panic disorder is relatively unexplored. Alterations within brain stress systems that secondarily influence the immune system have been documented. A recent report indicated elevations of serotonin (5-HT) and ganglioside antibodies in patients with primary fibromyalgia, a condition with documented associations with panic disorder. In line with our interest in dysregulated 5-HT systems in panic disorder (PD), we wished to assess if antibodies directed at the 5-HT system were elevated in patients with PD in comparison to healthy volunteers. Sixty-three patients with panic disorder and 26 healthy volunteers were diagnosed by the SCID. Employing ELISA, we measured anti-5-HT and 5-HT anti-idiotypic antibodies (which are directed at 5-HT receptors). To include all subjects in one experiment, three different batches were run during the ELISA. Plasma serotonin anti-idiotypic antibodies: there was a significant group effect [patients > controls (p = .007)] and batch effect but no interaction. The mean effect size for the three batches was .76. Following Z-score transformation of each separate batch and then combining all scores, patients demonstrated significantly elevated levels of plasma serotonin anti-idiotypic antibodies. Neither sex nor age as covariates affected the significance of the results. There was a strong correlation between anti-serotonin antibody and serotonin anti-idiotypic antibody measures. Plasma anti-serotonin antibodies: there was a significant diagnosis effect [patients > controls (p = .037)]. Mean effect size for the three batches was .52. Upon Z-score transformation, there was a diagnosis effect with antibody elevations in patients. Covaried for sex and age, the result falls below significance to trend levels. The data raise the possibility that psychoimmune dysfunction, specifically related to the 5-HT system, may be present in PD. Potential interruption of 5-HT neurotransmission through autoimmune mechanisms may be of pathophysiologic significance in certain patients with panic disorder. It remains to be demonstrated if the peripheral autoimmunity is representative of CNS 5-HT neuronal alterations. Replication appears warranted.  (+info)

Genetic linkage of IgA deficiency to the major histocompatibility complex: evidence for allele segregation distortion, parent-of-origin penetrance differences, and the role of anti-IgA antibodies in disease predisposition. (4/2060)

Immunoglobulin A (IgA) deficiency (IgAD) is characterized by a defect of terminal lymphocyte differentiation, leading to a lack of IgA in serum and mucosal secretions. Familial clustering, variable population prevalence in different ethnic groups, and a predominant inheritance pattern suggest a strong genetic predisposition to IgAD. The genetic susceptibility to IgAD is shared with a less prevalent, but more profound, defect called "common variable immunodeficiency" (CVID). Here we show an increased allele sharing at 6p21 in affected members of 83 multiplex IgAD/CVID pedigrees and demonstrate, using transmission/diseqilibrium tests, family-based associations indicating the presence of a predisposing locus, designated "IGAD1," in the proximal part of the major histocompatibility complex (MHC). The recurrence risk of IgAD was found to depend on the sex of parents transmitting the defect: affected mothers were more likely to produce offspring with IgAD than were affected fathers. Carrier mothers but not carrier fathers transmitted IGAD1 alleles more frequently to the affected offspring than would be expected under random segregation. The differential parent-of-origin penetrance is proposed to reflect a maternal effect mediated by the production of anti-IgA antibodies tentatively linked to IGAD1. This is supported by higher frequency of anti-IgA-positive females transmitting the disorder to children, in comparison with female IgAD nontransmitters, and by linkage data in the former group. Such pathogenic mechanisms may be shared by other MHC-linked complex traits associated with the production of specific autoantibodies, parental effects, and a particular MHC haplotype.  (+info)

Antiidiotypic antibody recognizes an amiloride binding domain within the alpha subunit of the epithelial Na+ channel. (5/2060)

We previously raised an antibody (RA6.3) by an antiidiotypic approach which was designed to be directed against an amiloride binding domain on the epithelial Na+ channel (ENaC). This antibody mimicked amiloride in that it inhibited transepithelial Na+ transport across A6 cell monolayers. RA6.3 recognized a 72-kDa polypeptide in A6 epithelia treated with tunicamycin, consistent with the size of nonglycosylated Xenopus laevis alphaENaC. RA6.3 specifically recognized an amiloride binding domain within the alpha-subunit of mouse and bovine ENaC. The deduced amino acid sequence of RA6.3 was used to generate a three-dimensional model structure of the antibody. The combining site of RA6.3 was epitope mapped using a novel computer-based strategy. Organic residues that potentially interact with the RA6.3 combining site were identified by data base screening using the program LUDI. Selected residues docked to the antibody in a manner corresponding to the ordered linear array of amino acid residues within an amiloride binding domain on the alpha-subunit of ENaC. A synthetic peptide spanning this domain inhibited the binding of RA6.3 to alphaENaC. This analysis provided a novel approach to develop models of antibody-antigen interaction as well as a molecular perspective of RA6.3 binding to an amiloride binding domain within alphaENaC.  (+info)

Elimination of the immunogenicity of therapeutic antibodies. (6/2060)

The immunogenicity of therapeutic Abs limits their long-term use. The processes of complementarity-determining region grafting, resurfacing, and hyperchimerization diminish mAb immunogenicity by reducing the number of foreign residues. However, this does not prevent anti-idiotypic and anti-allotypic responses following repeated administration of cell-binding Abs. Classical studies have demonstrated that monomeric human IgG is profoundly tolerogenic in a number of species. If cell-binding Abs could be converted into monomeric non-cell-binding tolerogens, then it should be possible to pretolerize patients to the therapeutic cell-binding form. We demonstrate that non-cell-binding minimal mutants of the anti-CD52 Ab CAMPATH-1H lose immunogenicity and can tolerize to the "wild-type" Ab in CD52-expressing transgenic mice. This finding could have utility in the long-term administration of therapeutic proteins to humans.  (+info)

Cleavage of transcription factor SP1 by caspases during anti-IgM-induced B-cell apoptosis. (7/2060)

Apoptosis is instrumental in the processes generating the diversity of the B-cell repertoire. Autoreactive B-cells are eliminated by anti-IgM crosslinking after encountering self-antigens, but precise mechanisms leading to B-cell apoptosis are still not well understood. We report here the cleavage of the transcription factor SP1 in the human Burkitt lymphoma cell line BL60 during anti-IgM-induced apoptosis. Western blot analysis revealed two cleavage products of approximately 68 kDa and 45 kDa after induction of apoptosis. Cleavage could be completely inhibited by zDEVD-fmk, an inhibitor specific for caspase 3-like proteases. In-vitro cleavage of recombinant SP1 by recombinant caspase 3 (CPP32) or caspase 7 (Mch 3) results in similar cleavage products as those observed in vivo. Recombinant caspase 6 (Mch 2) primarily generates a 68-kDa cleavage product, as observed after calcium ionophore (CaI) induced B-cell apoptosis. In contrast, caspase 1 (ICE) did not cleave SP1 in vitro. The time course of SP1 cleavage during anti-IgM-induced apoptosis is paralleled by an increase of caspase activity measured by DEVD-p-nitroanilide (DEVD-pNA) cleavage. DNA band-shift assays revealed a decrease in the intensity of the full length SP1/DNA complex and an increase in the intensity of a smaller complex due to the binding of one SP1 cleavage product. By Edman sequencing we could identify a caspase 3 cleavage site after Asp584 (D584AQPQAGR), generating a 22-kDa C-terminal SP1 protein fragment which still contains the DNA binding site. Our results show the cleavage of the human transcription factor SP1 in vivo and in vitro, underlining the central role of caspase 3-like proteases during the process of anti-IgM-induced apoptosis.  (+info)

Extracellular signal-regulated kinases regulate leukotriene C4 generation, but not histamine release or IL-4 production from human basophils. (8/2060)

Human basophils secrete histamine and leukotriene C4 (LTC4) in response to various stimuli, such as Ag and the bacterial product, FMLP. IgE-mediated stimulation also results in IL-4 secretion. However, the mechanisms of these three classes of secretion are unknown in human basophils. The activation of extracellular signal-regulated kinases (ERKs; ERK-1 and ERK-2) during IgE- and FMLP-mediated stimulation of human basophils was examined. Following FMLP stimulation, histamine release preceded phosphorylation of ERKs, whereas phosphorylation of cytosolic phospholipase A2 (cPLA2), and arachidonic acid (AA) and LTC4 release followed phosphorylation of ERKs. The phosphorylation of ERKs was transient, decreasing to baseline levels after 15 min. PD98059 (MEK inhibitor) inhibited the phosphorylation of ERKs and cPLA2 without inhibition of several other tyrosine phosphorylation events, including phosphorylation of p38 MAPK. PD98059 also inhibited LTC4 generation (IC50 = approximately 2 microM), but not histamine release. Stimulation with anti-IgE Ab resulted in the phosphorylation of ERKs, which was kinetically similar to both histamine and LTC4 release and decreased toward resting levels by 30 min. Similar to FMLP, PD98059 inhibited anti-IgE-mediated LTC4 release (IC50, approximately 2 microM), with only a modest effect on histamine release and IL-4 production at higher concentrations. Taken together, these results suggest that ERKs might selectively regulate the pathway leading to LTC4 generation by phosphorylating cPLA2, but not histamine release or IL-4 production, in human basophils.  (+info)

A monoclonal anti-idiotypic antibody specific to a human IgG 1 type monoclonal antibody possessing specificity to nicotinic acetylcholine receptor; a method for the production of the aforementioned monoclonal anti-idiotypic antibody by the steps of immunizing an animal with a human IgG 1 type monoclonal antibody specific to nicotinic acetylcholine receptor, collecting antibody-producing cells from the animal, fusing the collected cells with neoplastic cells, selecting from the product of fusion a hybridoma capable of producing a monoclonal anti-idiotypic antibody specific to the human IgG 1 type monoclonal antibody possessing specificity to nicotinic acetylcholine receptor, propagating the selected hybridoma thereby giving rise to said monoclonal anti-idiotypic antibody, and collecting the produced monoclonal anti-idiotypic antibody; and use of the monoclonal anti-idiotypic antibody as a reagent and as an adsorbent.
A monoclonal anti-idiotypic antibody specific to a human IgG1 type monoclonal antibody possessing specificity to nicotinic acetylcholine receptor; a method for the production of the aforementioned monoclonal anti-idiotypic antibody by the steps of immunizing an animal with a human IgG1 type monoclonal antibody specific to nicotinic acetylcholine receptor, collecting antibody-producing cells from the animal, fusing the collected cells with neoplastic cells, selecting from the product of fusion a hybridoma capable of producing a monoclonal anti-idiotypic antibody specific to the human IgG1 type monoclonal antibody possessing specificity to nicotinic acetylcholine receptor, propagating the selected hybridoma thereby giving rise to said monoclonal anti-idiotypic antibody, and collecting the produced monoclonal anti-idiotypic antibody; and use of the monoclonal anti-idiotypic antibody as a reagent and as an adsorbent.
Specific Interference with the Determination of the Tumour-Associated Glycoprotein 72 by Human Anti-Idiotypic Antibodies Formed after Treatment with the Anti-Tumour-Associated Glycoprotein 72 Antibody ...
Two mouse monoclonal anti-anti-idiotopic antibodies (anti-anti-Id, Ab3), AF14 and AF52, were prepared by immunizing BALByc mice with rabbit polyclonal antiidiotypic antibodies (anti-Id, Ab2) raised against antibody D1.3 (Ab1) specific for the antigen hen egg lysozyme. AF14 and AF52 react with an internal image monoclonal mouse anti-Id antibody E5.2 (Ab2), previously raised against D1.3, with affinity constants (1.0 3 109 M21 and 2.4 3 107 M21, respectively) usually observed in secondary responses against protein antigens. They also react with the antigen but with lower affinity (1.8 3 106 M21 and 3.8 3 106 M21). This pattern of affinities for the anti-Id and for the antigen also was displayed by the sera of the immunized mice. The amino acid sequences of AF14 and AF52 are very close to that of D1.3. In particular, the amino acid side chains that contribute to contacts with both antigen and anti-Id are largely conserved in AF14 and AF52 compared with D1.3. Therapeutic immunizations against different
Neuroblastoma treatment with chimeric anti-disialoganglioside GD2 antibody ch14.18 showed objective anti-tumor responses. Production of anti-idiotypic antibodies (Ab2) against ch14.18 (Ab1) in some cases was positively correlated with a more favorable prognosis. According to Jernes network theory, a subset of anti-idiotypic antibodies (Ab2beta) form an internal image of the antigen and induce antibodies (Ab3) against the original antigen. The molecular origin of the anti-idiotypic antibody response in tumor patients was not investigated previously. To clone anti-idiotypic antibodies, B-cells of a ch14.18-treated neuroblastoma patient with Ab2 serum reactivity were used to construct antibody phage display libraries (Methods described in Arthritis Rheum. 2000, 43:2722-2732). Upon repetitive selection of lambda and kappa Fab-phage display libraries on target antigens ch14.18 and the murine equivalent 14G2a, positive binders were enriched. Selected Ab2-clones GK2 and GK8 as well as another 38 ...
Many monoclonal antibodies that react with the lacto-N-fucopentaose III (LNF III) antigenic determinant, Gal beta 1-4(Fuc alpha 1-3)GlcNAc beta 1-3Gal beta 1-4Glc, have been described recently. The terminal trisaccharide of this determinant, fucosyllactosamine, is present on glycolipids and glycoproteins and on the surface of granulocytes, monocytes, and other cells. To study the structural and genetic diversity of these antibodies, syngeneic anti-idiotypic monoclonal antibodies were produced in BALB/c mice against PMN 6, a monoclonal antibody directed against this sequence. Anti-idiotypic antibodies 6B1 and 6C4 reacted with 50% of a panel of 20 anti-LNF III monoclonal antibodies, whereas 6A3 reacted strongly only with PMN 6. This indicates that the determinants recognized by 6C4 and 6B1 represent major cross-reactive idiotopes of this family of antibodies. The binding of idiotypic antibodies to a glycolipid bearing this antigenic determinant was completely inhibited by the three anti-idiotypic ...
Cell surface receptors for the 86-kDa glycoprotein (gp86) of human cytomegalovirus (HCMV) were identified by using two monoclonal anti-idiotype antibodies that bear the internal image of gp86. These antibodies bound to cells permissive for HCMV infection by both ELISA and immunofluorescence assay and inhibited HCMV plaque formation in human embryonic lung (HEL) cells. Immunoblot analysis showed specific binding of both internal image anti-idiotype antibodies as well as gp86 to an HEL cell membrane protein with an approximate molecular mass of 92.5 kDa. In addition, immunoprecipitation of radiolabeled membrane and cell surface proteins from human foreskin tissue, human foreskin fibroblasts, or HEL cells showed specific binding of anti-idiotype antibody predominantly to the 92.5-kDa protein.. ...
Results Anti-infliximab antibodies were detected in 21 patients (median: 131 ng/ml, range 10-200 ng/ml). Infliximab levels were weak in 25 patients (median: 0.16 microg/ml, range: ,0.1-0.41 microg/ml). In 20/25 patients (80%), the weak level of infliximab was associated to the presence of anti-infliximab antibodies. Interestingly, in sera of two patients obtained more than one year after the last infliximab perfusion, anti-infliximab antibodies were still present. Elevated infliximab levels were associated to the presence of anti-infliximab antibodies in only one patient. In 6 patients, the loss of infliximab response was not explained by the presence of anti-TNF antibodies or weak infliximab levels, suggesting that a switch to another biological agent could be more efficient than to another TNF inhibitor.. ...
Rose, L M.; Goldman, M; and Lambert, P, The production of anti-idiotypic antibodies and of idiotype- -anti-idiotype immune complexes after polyclonal activation induced by bacterial lps. (1982). Subject Strain Bibliography 1982. 2727 ...
B-cell lymphomas express surface immunoglobulin (immunoglobulin) containing unique idiotypic (idiotype) determinants which may be exploited as tumor specific markers. The inventor has produced murine monoclonal antibodies (MAbs) reactive with the idiotype marker derived from 67 patients with low grade, follicular, small cleaved cell lymphoma. Out of 199 monoclonal antibodies, 47 (24%) were found to react with pooled normal human serum immunoglobulin in concentrations ranging from 0.6 μg/ml to 160 μg/ml. Of these 40 monoclonal antibodies, 90% cross-reacted with idiotype present in normal serum in levels |50 μg/ml. Thirty-two of these anti-idiotypes were directed against a shared idiotope expressed on another patients lymphoma cells. The frequency of shared idiotope expression defined by each antibody ranged from 0.26% to 3.9% of the B-cell lymphomas tested. A panel of five anti-idiotype antibodies reacted with 80% of AIDS associated lymphomas. Based on the reactivity with these monoclonal antibodies,
Immunogenicity of Protein-315 and its Fab fragment (Fab-315) for isologous and heterologous strains of mice was investigated by comparing the characteristics of the anti-idiotypic response produced in BALB/c and A/J mice. The ability of these anti-idiotypic antisera to compete with DNP-lys for the ligand-binding site of Protein-315 were analyzed by comparing their hapten inhibition curves.. The anti-idiotypic responses of BALB/c mice to Protein-315 and to Fab-315 were similar to one another with regard to antibody specificity and sensitivity to inhibition by hapten, suggesting that both forms were equally immunogenic in inbred BALB/c mice. This observation indicates that the Fc portion of Protein-315 is not essential for the induction of anti-idiotypic response. Both BALB/c and A/J mice recognized the same antigenic determinants on Fab-315 since the anti-idiotypic antibodies produced by these animals were indistinguishable with regard to their interaction with Protein-315, Fv-315, and ...
Anti-idiotype vaccination represents an innovative approach to target tumor-associated antigen-expressing cells. This approach comes directly from Jernes idiotypic network theory, which postulates that due to the huge potentiality for diversity of the immunoglobulin variable regions, the idiotype repertoire can mimic the universe of self and foreign epitopes [1].. NeuGc-containing gangliosides are attractive targets for cancer immunotherapy because these glycolipids are non-self antigens in humans [2, 3]. In contrast, they have been detected in different human tumors by antibodies and chemical analysis [4-6]. Recent experimental data suggest that N-glycolyl-GM3 ganglioside (NeuGcGM3) is relevant for tumor biology [7].. mAb-1E10 [8] is an IgG1 anti-idiotype (Ab2) mAb obtained by immunizing Balb/c mice with mAb-P3 (Ab1) [9] coupled to keyhole limpet hemocyanin (KLH) in the presence of Freunds adjuvant. This Ab2 inhibited the binding of mAb-P3 to NeuGcGM3 ganglioside. mAb-1E10 induced an ...
TY - JOUR. T1 - Induction of murine B cell proliferation by insolubilized anti-immunoglobulins. AU - Pure, E.. AU - Vitetta, E.. PY - 1980/1/1. Y1 - 1980/1/1. N2 - This study demonstrates that intact antibodies specific for μ, δ, or light chains when coupled to Sepharose are effective in delivering proliferative signals to B cells. Furthermore, using Sepharose-coupled anti-δ antibodies, we have shown that hybridoma anti-δ (that is not active as soluble protein) is as effective as rabbit anti-δ in activating murine B cells. However, antibodies directed against two other surface molecules, I-A and H-2K, were not mitogenic. Thus, sIgM and sIgD are comparable in their ability to transmit a proliferative signal to the B cell and sIg seems to play a unique role in this regard.. AB - This study demonstrates that intact antibodies specific for μ, δ, or light chains when coupled to Sepharose are effective in delivering proliferative signals to B cells. Furthermore, using Sepharose-coupled anti-δ ...
Understanding the molecular mechanisms of immunological memory assumes importance in vaccine design. We had earlier hypothesized a mechanism for the maintenance of immunological memory through the operation of a network of idiotypic and anti-idiotypic antibodies (Ab2). Peptides derived from an internal image carrying anti-idiotypic antibody are hypothesized to facilitate the perpetuation of antigen specific T cell memory through similarity in peptide-MHC binding as that of the antigenic peptide. In the present work, the existence of such peptidomimics of the antigen in the Ab2 variable region and their similarity of MHC-I binding was examined by bioinformatics approaches. The analysis employing three known viral antigens and one tumor-associated antigen shows that peptidomimics from Ab2 variable regions have structurally similar MHC-I binding patterns as compared to antigenic peptides, indicating a structural basis for memory perpetuation. (C)) 2007 Elsevier Inc. All rights reserved.. ...
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TY - JOUR. T1 - The physiology of anti-idiotypic interactions. T2 - From clonal to paratopic selection. AU - Greally, John M.. PY - 1991. Y1 - 1991. N2 - On theoretical and experimental grounds, it has been proposed that the idiotypes of immunoglobulins and of T cell receptors are composed of multiple paratopes, as opposed to a single paratope and several idiotopes. This necessitates a revision of some of the basic principles of anti-idiotypic reactions. It is also possible to infer the presence of the same or similar paratopes on different idiotypes. A paratope cannot therefore be regarded as restricted to or unique on an idiotype. For these reasons, the perception of immunological specificity in terms of clonal units is misleading. This review proposes instead that the physiological unit of immunological specificity and regulation is the paratope. This essential alteration in the perception of the immune system is referred to as paratopic selection. The approach is assessed in terms of ...
Antibodies directed against IgG and DNA are found in the sera of autoimmune MRL/Mp lpr/lpr mice. Little is known of the molecular mechanisms underlying expression of such autoantibodies. We have investigated the binding diversity and pattern of VH gene expression in a panel of murine anti-IgG antibodies. We constructed eight hybridoma clones secreting IgM antibodies that bound to mouse IgG by using spleen cells from MRL/Mp lpr/lpr mice varying in age from 4 to 15 wk; one clone was derived from a 32-wk-old MRL +/+ mouse. The monoclonal IgM products exhibited varying binding specificities for intact mouse IgG, fragments of mouse IgG [Fc, Fab, (Fab)2], and heterologous IgG. Two of these antibodies crossreacted with B and/or Z DNA. Probes from seven of eight identified mouse VH gene families (7183, S107, Q52, J558, J606, 36-60, and 3609) were hybridized under high-stringency conditions with cytoplasmic RNA blots from each clone. Six clones hybridized only with the probe from the five-member 36-60 ...
Injections may be a helpful add-on treatment for certain people with severe allergic asthma. Learn more about how it works, how much it costs, and some of the potential side effects.
E. S. Aleksandrova, F. Koralewski, M. I. Titov, A. V. Demin, A. N. Ignatova, A. V. Kozyr, A. V. Kolesnikov, A. Tramontano, S. Paul, D. Thomas, A. G. Gabibov, A. Friboulet ...
Cosenza, H; Augustin, A; and Julius, M H., Induction and characterization of autologous anti-idiotypic antibodies. (1977). Subject Strain Bibliography 1977. 3483 ...
We conceived this study to challenge, in the light of current knowledge, the tacit assumption that omalizumab therapy is of no clinical benefit in conventionally defined, non-atopic asthmatics. In lieu of a large, long-term, conventional clinical trial in the first instance, which would have been difficult to fund given the paucity of supportive evidence, we elected to provide proof of concept that omalizumab therapy of these patients reduces IgE expression and IgE sensitisation of target cells within the bronchial mucosa while exerting a favourable effect on lung function in the short term, as assessed by changes in FEV1. We obtained bronchial biopsies before and after 12-14 weeks of treatment with omalizumab or placebo while maintaining existing therapy, reasoning that any differences in mucosal cell populations between the active and placebo cohorts observed under these conditions would be attributable to omalizumab. We set changes in mucosal IgE+ cells as a co-primary outcome measure, ...
A 13-amino-acid motif in the cytoplasmic domain of FcyRIIB modulates B-cell receptor signalling. Nature (London) 368, 7 0 - 7 3 . , and Lamoyi, E. (1981). Implications of the presence of an internal image of the antigen in anti-idiotypic antibodies: Possible application to vaccine production. Clin. Immunol. Immunopathol. 21, 397. Noelle, R. J . , and Snow, E. C. (1991). T helper cell-dependent B cell activation. FASEB J. 5, 2770-2776. Nossal, G. J. V. (1994). Negative selection of lymphocytes. Cell 76, 2 2 9 - 2 3 9 . Multifactorial nature of human immunodeficiency virus disease: Implications for therapy. Science 262, 1011-1018. Finkelman, F. , Holmes, J . , Katona, I. , Urban, J. F . , Beckmann, M. , Park, L. , Schooley, K. , Coffman, R. L . , Mosmann, T. , and Paul, W. E. (1990). Lymphokine control of in vivo immunoglobulin isotype selection. Annu. Rev. Immunol. 8, 3 0 3 - 3 3 3 . Fiorentino, D. F . , Bond, M. , and Mossman, T. R. (1989). Two types of mouse helper T cell. IV. Th2 clones ...
Results 33 children (22 male) aged 5-16 years were commenced on omalizumab. At 16 weeks there were significant improvements in mini-AQLQ; ACT; FENO; maintenance OCS dose; severe exacerbations and UHCVs.. 20/33 (60.6%) children continued omalizumab beyond the initial 16 weeks (up to 192 weeks). Compared to those who discontinued, at baseline these children had higher mini-AQLQ (4.28 vs. 3.05) and ACT (11 vs. 8), were younger (11 vs. 13 years) and were more likely to have been admitted to hospital (57.9% vs. 0%) and have had a severe exacerbation (95% vs. 50%) in the 16 weeks before starting omalizumab. Maximal reduction in number of exacerbations and hospital admissions was evident at 32 weeks; this was maintained for up to 144 weeks (Figure 1).. ...
Human Anti-Golimumab Antibody, clone AbD25455 is an anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug goli
|strong|Human Anti-Nivolumab Antibody, clone AbD30255|/strong| is a paratope specific, inhibitory anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug nivolumab. The antib…
Our laboratory is developing cancer vaccines that are being evaluated in animal models for their protective activity against tumors before they are administered to cancer patients. The vaccines are composed of antibodies mimicking tumor antigens (anti-idiotypic antibodies) or the antigens expressed in viruses (recombinant adeno- or vaccinia-viruses) and are selected based on their high probability of inducing both humoral and cellular immune responses in patients. The animal models we have developed for preclinical evaluation of the vaccines closely mimic the condition in cancer patients. In other studies which have reached clinical trials, we are evaluating the vaccinated patients immune responses to their tumors ...
Omalizumab Omalizumab? Therapeutic monoclonal antibody Source Humanized Target IgE Identifiers CAS number 242138-07-4 ATC code R03DX05 PubChem  ? DrugBank
Mean serum IFX trough level was 4.4+ 4.7mg/ml in 430 samples from 122 patients. The level was significantly lower in symptomatic phase (n=73, 2.5±5.3 μg/ml) than in the asymptomatic phase (n=357, 4.9 ± 4.6μg/ml). ROC analysis determined the cut-off level was 0.93μg/ml. Patient-based analysis revealed that the trough level was lower in Group C+D (1.3±3.3μg/ml) than Group A (4.2±4.4μg/ml) and B (5.4±5.7μg/ml), whereas administration interval was significantly shorter in Group B (7.2±1.1 wk) and C+D (6.8±1.5 wk) than Group A (8.2±0.9 wk). ATI(+) was found in 18 (11.3%) of all patients. The frequency was significantly higher in Group C+D (5/10, 50%) and E (4/8, 50%) than Group A (3/75, 4%) and B (6/38, 16%). Thus, unfavorable clinical responses were associated with low trough level and positive ATI. Muitivariate analysis using logistic regression model revealed association of therapeutic failure (Group C+D and E) with female, positive ATI, and infusion reaction (IR). Moreover, ATI was ...
T cell vaccination (TCV) activates Tregs of 2 kinds: anti-idiotypic (anti-id) and anti-ergotypic (anti-erg). These regulators furnish a useful view of the physiology of T cell regulation of the immune response. Anti-id Tregs recognize specific effector clones by their unique TCR CDR3 peptides; anti-id networks of CD4+ and CD8+ Tregs have been described in detail. Here we shall focus on anti-erg T regulators. Anti-erg T cells, unlike anti-id T cells, do not recognize the clonal identity of effector T cells; rather, anti-erg T cells recognize the state of activation of target effector T cells, irrespective of their TCR specificity. We consider several features of anti-erg T cells: their ontogeny, subset markers, and target ergotope molecules; mechanisms by which they regulate other T cells; mechanisms by which they get regulated; and therapeutic prospects for anti-erg upregulation and downregulation.. ...
The responsiveness to IL-4 with and without costimulation with anti-IgM antibodies or phorbolester was studied in 35 cases of low grade non-Hodgkin Iymphoma by analyzing enhancement of CD23 and HLA dass li expression. The predominant phenotype responds directly to IL-4. Separate differentiation states can be distinguished according to coordinate or differential upregulation of CD23 and HLA dass II molecules by IL-4 alone, and differences in responsiveness to anti-IgM antibodies. A particular subgroup of B-lymphoma cells defines a separate stage of B-eeil differentiation. They fail to express high affinity binding sites for IL-4 and accordingly do not respond to IL-4- mediated signals. Cross-linking membrane lgM receptors or direct activation of protein kinase C via phorbolester induces IL-4 receptor expression and subsequent IL-4 reactivity ...
The responsiveness to IL-4 with and without costimulation with anti-IgM antibodies or phorbolester was studied in 35 cases of low grade non-Hodgkin Iymphoma by analyzing enhancement of CD23 and HLA dass li expression. The predominant phenotype responds directly to IL-4. Separate differentiation states can be distinguished according to coordinate or differential upregulation of CD23 and HLA dass II molecules by IL-4 alone, and differences in responsiveness to anti-IgM antibodies. A particular subgroup of B-lymphoma cells defines a separate stage of B-eeil differentiation. They fail to express high affinity binding sites for IL-4 and accordingly do not respond to IL-4- mediated signals. Cross-linking membrane lgM receptors or direct activation of protein kinase C via phorbolester induces IL-4 receptor expression and subsequent IL-4 reactivity ...
我們的研究主軸是利用抗體工程技術來開發新藥。這些以抗體為結構基礎的藥物,主要標的牽涉於IgE引致的過敏反應過程。我們也積極從事開發可提升抗體工程 的數種創新技術平台。其中一項研究計畫就是要發展人源化、高親和力,及對人體膜IgE分子內CεmX具結合特異性的抗體,以用來控制表現IgE的B淋巴細 胞。CεmX是張教授的研究群發現的;它是一含有具特異序列的52個氨基酸長的胜肽區段。如發展成功,anti-CεmX可與張教授發明的anti-IgE,如已在美國等國核准上市用於嚴重哮喘的omalizumab(商名Xolair),共同使用。. ...
ForteBios Anti-Murine IgG Quantitation biosensors provide a rapid method for precise quantitation of murine immunoglobulins from crude lysates and cell culture supernatants. For Determination of Antibody Concentration
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Rabbit Polyclonal ID4 antibody N-Term for WB. Published in 2 Pubmed References. Order this anti-ID4 antibody. | Product number ABIN2780374
Abcam provides specific protocols for Anti-gamma H2A.X (phospho S139) antibody - ChIP Grade (ab2893) : ChIP protocols, Immunoprecipitation protocols…
Anti-gamma H2A.X (phospho S139) antibody [9F3] (ab26350) has been cited in 47 publications. References for Human, Mouse, Rat, Dog, Pig in ICC, ICC/IF, IF, IHC…
The progression of this project, as well as the outcomes, will be extremely relevant to the public and scientific researchers since Severe Allergic Asthma is a common disease worldwide. AA is well documented and the public already have a great deal of understanding and so are more likely to be interested in new advances in research related to this condition. The objectives of this work package are to ...
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TY - JOUR. T1 - Host Anti-antibody Responses Following Adeno-associated Virus-mediated Delivery of Antibodies Against HIV and SIV in Rhesus Monkeys. AU - Martinez-Navio, José M.. AU - Fuchs, Sebastian P.. AU - Pedreño-López, Sònia. AU - Rakasz, Eva G.. AU - Gao, Guangping. AU - Desrosiers, Ronald C.. PY - 2015/10/7. Y1 - 2015/10/7. N2 - Long-term delivery of antibodies against the human immunodeficiency virus (HIV) using adeno-associated virus (AAV) vectors is a promising approach for the prevention or treatment of HIV infection. However, host antibody responses to the delivered antibody are a serious concern that could significantly limit the applicability of this approach. Here, we describe the dynamics and characteristics of the anti-antibody responses in monkeys that received either rhesus anti-simian immunodeficiency virus (SIV) antibodies (4L6 or 5L7) in prevention trials or a combination of rhesusized human anti-HIV antibodies (1NC9/8ANC195/3BNC117 or 10-1074/10E8/3BNC117) in therapy ...
Methods: Consecutive patients with severe asthma disease (n=15; Group IA, pretreatment and Group IB, post-treatment) underwent omalizumab treatment. Control group was age- and sex-matched including 25 healthy in Group II. Blood samples from both the groups were taken during their first visit (Group IA and II) and then after 12 months of treatment in asthmatic patients (Group IB). Serum levels of homocysteine (Hcy), eosinophil cationic peptide (ECP), 25-hydroxyvitamin D (25(OH)D), interleukin-1β (IL-1β), soluble OX2 (sCD200) and clinical follow-up tests including fractional exhaled nitric oxide (FeNO), asthma control test (ACT), and pulmonary function tests were evaluated ...
Anti-IgE (omalizumab) has been shown to be an effective add-on therapy for patients with allergic severe asthma. In this observational study patients aged over 18 year with uncontrolled severe persistent asthma are selected for add-on therapy with omalizumab. Patients were on high dose of ICS and had a documented history of 2-6 exacerbations requiring treatment with systemic corticosteroids ( with ,15 mg/day prednisone or other medications at similar dose, for at least 3 days). The individual dose and frequency of omalizumab administration is assessed from the dosing table. Lung function tests and asthma questionnaires (ACQ, AQLQ and RQLQ) are used in the aim of assessing clinical improvement after omalizumab treatment. Induced sputum (IS) and exhaled breath condensate (EBC) are used as a simple non-invasive methods for monitoring cellular and biochemical changes in the airways. Total blood eosinophil count, IS cytology, IS and EBC periostin and IL-6 concentrations are measured. Analyses are ...
General It is not known whether Simulect use will have a long-term effect on the ability of the immune system to respond to antigens first encountered during Simulect -induced immunosuppression. Immunogenicity Of renal transplantation patients treated with Simulect and tested for anti-idiotype antibodies, 4/339 developed an anti-idiotype antibody response, with no deleterious clinical effect upon the patient. In none of these cases was there evidence that the presence of anti-idiotype antibody accelerated Simulect clearance or decreased the period of receptor saturation. In Study 2, the incidence of human anti-murine antibody (HAMA) in renal transplantation patients treated with Simulect was 2/138 in patients not exposed to muromonab-CD3 and 4/34 in patients who subsequently received muromonab-CD3. The available clinical data on the use of muromonab-CD3 in patients previously treated with Simulect suggest that subsequent use of muromonab-CD3 or other murine anti-lymphocytic antibody preparations ...
Omalizumab, a humanized monoclonal anti-IgE antibody has the potential to alter allergen processing. Recently, it has been postulated the assessment of PHA-stimulated adenosine triphosphate (ATP) activity as maker of CD4+ T cells activity in peripheral blood cells. We present the case report of a 35-year-old woman with a history of chronic idiopathic urticaria and angioedema of 8 years of development with poor response to treatment. The patient was partially controlled with cyclosporine at doses of 100 mg/12 h. However, she was still developing hives daily. Finally treatment with omalizumab was started at dose of 300 mg every 2 weeks. The patient experienced a decrease in urticarial lesions 2 days after starting therapy. We also evaluated the effects of omalizumab therapy on the activity of peripheral blood CD4+ T cells from the patient, in order to determine the potential modification of anti-IgE therapy on the process of antigen presentation-recognition. Activity of CD4+ cells by ATP release was
Rabbit anti-idiotypic antibodies were prepared by injection of specifically purified anti-p-azobenzoate antibodies (D) from individual donor rabbits. Benzoate derivatives were found to be strong inhibitors of the reactions of D with anti-D antisera. There was a close correlation between the combining affinities of the benzoate derivatives used and their effectiveness as inhibitors. Compounds tested that are chemically unrelated to benzoate were ineffective. The results indicate either that the combining site of anti-benzoate antibody is part of an important idiotypic determinant, which is sterically blocked by hapten, or that the hapten induces a conformational change which alters idiotypic determinants not involving the active site. Such conformational changes, if they occur, must be restricted since hapten has little effect on the reactions of F(ab)2 fragments of anti-benzoate antibodies with antisera directed to rabbit fragment Fab and no detectable effect on reactions with antibodies ...
anti-Immunoglobulin G, Immunoglobulin M (IgG, IgM) (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP)) ABIN637975 from antibodies-online
Deterioration of asthma upon withdrawal of Xolair as demonstrated by meeting at least one of the following: *Worsening of pulmonary function tests (FEV1 ,80% predicted for height, age, and sex) and activity levels while off Xolair treatment; *Worsening of asthma exacerbations defined as doubling of inhaled steroid dose, increase in dose of oral steroids, or initiation of oral, intravenous, intramuscular, or subcutaneous (SC) steroids while off Xolair treatment; *Increased use of rescue medications while off Xolair treatment; *ER visits or unscheduled office visits for asthma that may or may not result in hospitalization while off Xolair ...
Omalizumab is a monoclonal anti-IgE antibody currently used in the treatment of chronic spontaneous urticaria (CSU) as a third-line option in cases refractory to treatment with the licensed dose of the first-line treatment or up to 4 times that dose. The introduction of omalizumab into the therapeutic arsenal for CSU brought about a real revolution in the management of this condition. However, the dose recommended in the Summary of Product Characteristics-based on the results of pivotal studies carried out as part of the approval process-is 300 mg/mo for a period of up to 6 months. In the present issue, the Catalan-Balearic working group presents a treatment algorithm to guide the use of omalizumab in the management of CSU. They discuss the various aspects of management related to the rational and evidence-based use of this drug, including candidate population, monitoring tools (Urticaria Activity Score 7 [UAS7] and Urticarial Control Test [UCT]), starting dose and dose adjustment as well as the ...
Case report: We present the case of a 48 year old woman, with severe persistent allergic asthma, despite level 4 (GINA) medical treatment, who initiated omalizumab in order to control her nocturnal symptoms and her frequent unscheduled medical visits. Before treatment and at 12 weeks: clinical evaluation with ACT was registered; lung function, FeNO, IgE and eosinophils were measured. Two-dimensional gas chromatography (GC ´GC-ToFMS) combined with headspace solid phase microextraction (HS-SPME) was applied to the untargeted study of the volatile metabolomic urine profile. ...
IL-12 R beta 1 Goat anti-Mouse, Polyclonal, R&D Systems™ 100μg; Unlabeled IL-12 R beta 1 Goat anti-Mouse, Polyclonal, R&D Systems™ Primary...
The study by Milgrom and colleagues is their second on the use of omalizumab (anti-IgE antibody) in the treatment of asthma and the third published in the past 3 months that addresses treatment with anti-IgE in large, multicenter asthma studies. Concurrent studies by Busse and colleagues (1) and Soler and colleagues (2) included , 500 adult patients aged 12 to 75 years and used medium to high-dose inhaled steroids (500 to 1200 µg/d of beclomethasone). They used a design similar to that of Milgrom and colleagues and achieved similar results in terms of steroid reduction and decreases in exacerbations. These studies, along with an earlier publication by Milgrom and colleagues (3), make a case for anti-IgE antibodies as adjunctive treatment for steroid-dependent patients with asthma. The advantages of anti-IgE over conventional therapies include once or twice-monthly subcutaneous injections and its tolerability with infrequent side effects. However, many questions remain. Although the association ...
Omalizumab - Get up-to-date information on Omalizumab side effects, uses, dosage, overdose, pregnancy, alcohol and more. Learn more about Omalizumab
CHO-Anti-Human IgE scFv stable cell line is clonally-derived from a CHO cell line, which has been transfected with an anti-human IgE scFv gene to allow expression of the scFv. It is an example of a cell line transfected using our proprietary CBTGS gene screening and amplification system.
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Author(s): Markus Brede ,Ulrich Behn Subject(s): CX.3 Category: Abstract:. The talk deals with modelling a subsystem of the immune system, the so-called idiotypic network. Idiotypic networks, a concept conceived by N.K. Jerne in 1974, are functional networks of interacting antibodies and B-cells. In principle, Jernes framework provides solutions to many issues in immunology, such as immunological memory, mechanisms for antigen recognition and the question of self/non-self discrimination. Explaining the interconnection between the elementary components local dynamics, network formation and architecture, and possible modes of global system function appears to be an ideal playground of statistical mechanics. We present a simple cellular automaton model based on a graph representation of the system. From a simplified description of idiotypic interactions rules for the random evolution of networks of occupied and empty sites on these graphs are derived. In certain biologically relevant parameter ...
THURSDAY, June 8, 2017 (HealthDay News) -- For patients with a significant response to allergen challenge, omalizumab induces protective effects against early (EAR) and late allergic reactions (LAR), according to a study published online June 5 in Allergy.. Jordis Trischler, M.D., from the University Hospital Frankfurt in Germany, and colleagues determined the time course of the early (EAR) and late allergic reaction (LAR). Ten patients with a significant response to allergen challenge were treated with omalizumab. At week one, two, four, and eight, bronchial allergen provocations were repeated.. The researchers observed a significant reduction in EAR after four weeks (change in forced expiratory volume in one second [ΔFEV1], 28 versus 11 percent; P , 0.001; exhaled nitric oxide, 86 versus 53 ppb; P , 0.05), and there was a reduction in basophil activation after two weeks (CD63 expression, 79 versus 32 percent; P , 0.05). After one week there was a reduction in LAR (ΔFEV1, 26 versus 13 ...
Container/Tube:. Preferred: Green-top (lithium heparin) tube. Acceptable: Gold-top serum gel tube or Plain Red-top tube. Specimen Volume: 170 uL of plasma or serum. Stability: Keep tubes stoppered and upright at all times. Do not use samples that have been stored at room temperature for longer than 8 hours. Tightly cap and refrigerate specimens at 2° to 8°C if the assay is not completed within 8 hours. Freeze samples at or below -20°C if the sample is not assayed within 48 hours. Freeze samples only once and mix thoroughly after thawing. Collection Instructions:. Note: 1. Human anti-mouse antibodies or other heterophile antibodies may be present in patient specimens. This assay has been specially formulated to minimize the effects of these antibodies, however results from patients known to have these antibodies should be carefully evaluated.. 2. Label specimen appropriately (plasma/serum).. ...
Goat anti-Mouse IgM Secondary Antibody, HRP conjugate from Invitrogen for Western Blot, Immunocytochemistry and Immunohistochemistry applications. Supplied as 2 mL purified secondary antibody (0.8 mg/ml) in PBS with 15mg/ml BSA and no preservative; pH 7.6.
Antibodies, Treatment, Human, Patients, Plasma, Serum, Igg, Immunoglobulin, Monoclonal Antibodies, Therapeutic, Anti-idiotype Antibodies, Breast, Breast Cancer, Calibration, Cancer, Elisa, Enzyme-linked Immunosorbent Assay, Horseradish, Horseradish Peroxidase, Immunoassay
The basic components of the diagnostic test systems are antigens and specific antibodies. The main objective of developing express tests for the diagnosis of bovine leukemia virus (BLV) is to obtain a virus antigen drug, which is very time-consuming to prepare. This problem can be solved by producing anti-idiotype antibodies that have a chemical structure identical to that of the viral antigen and does not require large expenditures to manufacture [1, 2 ...
Human IgG antibody, pre-adsorbed (TxRd) for ELISA, FACS, ICC/IF. Anti-Human IgG pAb (GTX27151) is tested in Human samples. 100% Ab-Assurance.
Learn about the potential side effects of Xolair (omalizumab). Includes common and rare side effects information for consumers and healthcare professionals.
This paper evaluates a representative sample of the best anti-ID and pro-ID publications and presents a conclusion as to the present state of the evidence and arguments regarding these positions. Published in Origins, n. 63.
Raffáč Š, Gombošová L, Gabzdilová J, Novotná L, Šajtyová K, Pekárová T, Kopolovets I, Tóth Š. Detection of anti-infliximab antibodies in Slovak IBD patients and its costs saving effect. Neuro Endocrinol Lett. 2017 Nov; 38(Suppl1): 5-9 ...
Specificityinternal regionStorage/StabilityAliquot and store at -20°C Minimize freezing and thawing More InformationImmunogenThe immunogen was a 12-residue peptide matching a sequence from the internal region of Mouse SETD2 See Accession Number s NP_054878 3 Formulation 0 5 mg/ml in TBS 0
Specificityinternal regionStorage/StabilityAliquot and store at -20°C Minimize freezing and thawing More InformationImmunogenThe immunogen was a 12-residue peptide matching a sequence from the internal region of Mouse Robo1 See Accession Number s NP_062286 2 Formulation 0 5 mg/ml in TBS 0
GenScriptRabbitAnti-PLCγ1(Ab-771)PolyclonalAntibodydetectsendogenouslevelsoftotalPLCγ1protein.FullNamePLCγ1Antibody(Ab-771),pAb,Rabbitabbreviated_name1PLC-γ1(Ab-771)AntibodyIgS
Pt presents to office with Asthma and is treated with Zolair. Since Zolair is considered a monoclonal antibody, what is the appropriate administration
CAR阳性表达率检测是CAR-T疗法质量控制的重要一环,常用的检测方案有利用Protein L、Anti-Fab抗体或靶点蛋白结合流式细胞术进行检测。其中,靶点蛋白结合流式细胞术的检测方案因其靶点特异性强的优势而备受青睐,许多业内人士预期靶点特异性的检测将会被监管机构纳入CAR-T质量控制监管考量的范畴。本文将对CAR阳性表达率检测相关案例做一汇总以供大家参考..
CU patients treated with Omalizumab, showed lower percentage of CD4+HLA-DR+CD38+[0,75(0,6-1,1)vs1,23(1,01-1,54)%,p,0,0001], CD4+DR+CD38-[1,7(0,7-3,15)vs3,07(2,5-4,7)%,p=0,0006] and higher percentage of CD8+DR-CD38+ [14±2 vs 8±0,5%,p=0,0001] and Th1 CM [12±0,8 vs 9±0,4%, p,0,0001] than HD.. ...
Euhesmaalbamala sp. n.: A-D Allotype male genital structures: A genital ventral B genital dorsal C S7+S8 ventral D S7+S8 dorsal. Scale bars: 200 µm.
... "anti-idiotypic antibody". If such is the case, the anti-idiotypic antibodies will be able to bind to the B lymphocyte receptor ... An anti-idiotypic monoclonal antibody was generated to possess an "internal image of cocaine". The anti-idiotypic antibody ... Ladjemi MZ (2012). "Anti-idiotypic antibodies as cancer vaccines: achievements and future improvements". Front Oncol. 2: 158. ... Because of the resemblance of anti-idiotypic antibodies to the original antigen, vaccine studies have been performed. These ...
Kennedy, R.; Eichberg, J.; Lanford, R.; Dreesman, G. (1986). "Anti-idiotypic antibody vaccine for type B viral hepatitis in ...
... yeast killer toxin-like anti-idiotypic antibodies". Journal of Immunology. 152 (6): 3175-82. PMID 8144911. Lowes KF, Shearman ... Several experiments suggest that antibodies that mimic the biological activity of killer toxins have application as antifungal ... March 1994). "Idiotypic intravaginal vaccination to protect against candidal vaginitis by secretory, ... "Therapeutic potential of yeast killer toxin-like antibodies and mimotopes". FEMS Yeast Research. 5 (1): 11-8. doi:10.1016/j. ...
"Anti-idiotypic binders for Trastuzumab validated in regulatory bioanalysis assay in collaboration with Covance". Avacta. ... "Antibody Alternatives". The Scientist Magazine®. Retrieved 2018-10-18. Sharma R, Deacon SE, Nowak D, George SE, Szymonik MP, ... Affimer reagents are suitable for use in biosensors, point-of-care diagnostics and as anti-idiotypic reagents in ... "Development and validation of anti-idiotypic Affmers to trastuzumab in a pharmacokinetic assay , Avacta". www.avacta.com. ...
Jorgensen, T.; Gaudernack, G.; Hannestad, K. (1977). "Production of BALB/c Anti-Idiotypic Antibodies Against the BALB/c Myeloma ... He further generated monoclonal antibodies specific for the hematopoietic stem cell marker, CD34. A joint collaboration with ... and generated monocyte-specific monoclonal antibodies (ID5) in hybridomas. Inspired by his research and without being ... Hospital and Baxter resulted in development of an instrument to isolate hematopoietic stem cells in large scale using anti-CD34 ...
... is an anti-idiotypic mouse monoclonal antibody that mimics NGc gangliosides, thus triggering an immune response ... March 2011). "Anti-NeuGcGM3 antibodies, actively elicited by idiotypic vaccination in nonsmall cell lung cancer patients, ... March 2002). "An anti-idiotype vaccine elicits a specific response to N-glycolyl sialic acid residues of glycoconjugates in ... 2012). "Racotumomab: an anti-idiotype vaccine related to N-glycolyl-containing gangliosides - preclinical and clinical data". ...
"Selection of human anti-HIV broadly neutralizing antibodies occurs within the context of a frozen 1F7-idiotypic repertoire". ... and B anti-anti-B (antiidiotypic) antibodies. The former stimulate anti-anti-B T cells and the latter stimulate anti-B T cells ... In the model IgG molecules are selected to have both anti-anti-(self MHC class II) and anti-anti-anti-(self MHC class II) ... of lymphocytes defined by co-selection of both anti-C and anti-anti-C lymphocytes and co-selection of anti-B and anti-anti-B ...
... of antibody response by mouse dendritic cells pulsed with tobacco mosaic virus or with rabbit antiidiotypic antibodies raised ... Urbain's idiotypic research showed a "dramatic enhancement of an antiviral immune response by dendritic cells," thus ... demonstrating the principle of a dendritic cell-enhanced immunity by means of anti-idiotype antibodies. He followed up with an ... The Immune Response Corporation filed a patent on this invention entitled: "Idiotypic Vaccination Against B-Cell Lymphoma" in ...
... anti-idiotypic antibodies, and vaccines to elicit immune responses to highly conserved viral proteins. Aquatic birds such as ... Once bound to these proteins, antibodies block virions from binding to cellular receptors, neutralizing the virus. The antibody ... B cells, a type of white blood cell, produce antibodies that bind to influenza antigens HA and NA (or HEF) and other proteins ... In other words, people exposed to a certain strain in childhood still possess antibodies to that strain at a reasonable level ...
... antibodies MeSH D12.776.377.715.548.114.071 - antibodies, anti-idiotypic MeSH D12.776.377.715.548.114.107 - antibodies, ... antibodies, bispecific MeSH D12.776.377.715.548.114.143 - antibodies, blocking MeSH D12.776.377.715.548.114.167 - antibodies, ... antibodies, helminth MeSH D12.776.377.715.548.114.191 - antibodies, heterophile MeSH D12.776.377.715.548.114.224 - antibodies, ... hiv antibodies MeSH D12.776.377.715.548.114.254.150.500 - htlv-i antibodies MeSH D12.776.377.715.548.114.254.150.510 - htlv-ii ...
... antibodies MeSH D12.776.124.486.485.114.071 - antibodies, anti-idiotypic MeSH D12.776.124.486.485.114.089 - antibodies, ... anti-idiotypic MeSH D12.776.124.790.651.114.107 - antibodies, archaeal MeSH D12.776.124.790.651.114.125 - antibodies, bacterial ... antibodies, bispecific MeSH D12.776.124.486.485.114.143 - antibodies, blocking MeSH D12.776.124.486.485.114.167 - antibodies, ... antibodies, helminth MeSH D12.776.124.486.485.114.191 - antibodies, heterophile MeSH D12.776.124.486.485.114.224 - antibodies, ...
... network theory proposed by Niels Kaj Jerne that describes the regulation of the immune system by anti-idiotypic antibodies ( ... antibodies that select for other antibodies). This class of algorithms focus on the network graph structures involved where ... antibodies (or antibody producing cells) represent the nodes and the training algorithm involves growing or pruning edges ... Immune Network Algorithms: Algorithms inspired by the idiotypic ... where selection is inspired by the affinity of antigen-antibody ...
... hormone recognition by an anti-idiotypic antibody, Science (1992) An αβ T Cell Receptor Structure at 2.5 Å and Its Orientation ... earliest research as a graduate student at Johns Hopkins University focused on understanding how anti-idiotyopic antibodies ... Engineered SIRPα variants as immunotherapeutic adjuvants to anticancer antibodies, Science (2013) Durable antitumor responses ... affinity antagonists of the receptor CD47 that potently enhance the antitumor effects of established therapeutic antibodies. ...
The anti-idiotypic antibody abagovomab in patients with recurrent ovarian cancer. A phase I trial of the AGO-OVAR. Ann Oncol. ... Abagovomab is a mouse anti-idiotype monoclonal antibody whose variable epitope mirrors a tumour antigen (CA-125) highly ... Immunological consolidation of ovarian carcinoma recurrences with monoclonal anti-idiotype antibody ACA125: immune responses ... "Medical News: ESGO: Anti-CA125 Fails to Slow Ovarian Cancer - in Meeting Coverage, ESGO from". MedPage Today. Retrieved 2011-09 ...
"Therapy of B-cell lymphoma with anti-CD20 antibodies can result in the loss of CD20 antigen expression". Clinical Cancer ... Clonal B-cells spontaneously mutate the idiotypic region of their immunoglobulin. This high mutation rate makes them prone to ... Anti-apoptotic proteins include Bcl-2, Bcl-XL, Bcl-w, Mcl-1. When anti-apoptotic family members are overexpressed, apoptotic ... Monoclonal antibodies are made by injecting human cancer cells into mice so that their immune systems create antibodies against ...
The Ag abbreviation stands for an antibody generator. Antigens are "targeted" by antibodies. Each antibody is specifically ... autologous and idiotypic or allogenic (homologous) antigens. Sometimes antigens are part of the host itself in an autoimmune ... "anti(body)-gen". Epitope - the distinct surface features of an antigen, its antigenic determinant. Antigenic molecules, ... In most cases, an adapted antibody can only react to and bind one specific antigen; in some instances, however, antibodies may ...
... network theory proposed by Niels Kaj Jerne that describes the regulation of the immune system by anti-idiotypic antibodies ( ... antibodies that select for other antibodies). This class of algorithms focus on the network graph structures involved where ... antibodies (or antibody producing cells) represent the nodes and the training algorithm involves growing or pruning edges ... Immune Network Algorithms: Algorithms inspired by the idiotypic ... where selection is inspired by the affinity of antigen-antibody ...
... anti-idiotypic antibodies. To keep things running along smoothly, the production of anti-idiotypic antibodies is automatic, ... I dont know what exactly the anti-idiotypic antibodies do with/to the antibodies to get rid of the extra antibodies. I dont ... anti-idiotypic antibodies actually means (I know I do). Well, it turns out that an idiotypic antibody is "immunologically ... You can also get cool things like anti-anti-idiotype antibodies. (Just read anti- as meta- and youll get the idea). ...
Order the anti-idiotypic antibodies you need and more today! ... ProSci is a leading provider of anti-idiotypic antibody ... Anti-Idiotypic Antibodies. The Antibody Variable Region. The idiotypic region of an antibody is defined as the unique set of ... These antibodies are known as anti-idiotypic antibodies and are particularly useful in their ability to specifically bind one ... Applications of Anti-Idiotypic Antibodies. The human or humanized nature of the majority of therapeutic monoclonal antibodies ...
... and collecting the produced monoclonal anti-idiotypic antibody; and use of the monoclonal anti-idiotypic antibody as a reagent ... monoclonal anti-idiotypic antibody by the steps of immunizing an animal with a human IgG1 type monoclonal antibody specific to ... a hybridoma capable of producing a monoclonal anti-idiotypic antibody specific to the human IgG1 type monoclonal antibody ... propagating the selected hybridoma thereby giving rise to said monoclonal anti-idiotypic antibody, ...
This antibody is able to induce an anti-idiotypic IgG response and B-T idiotypic cascade, even in the absence of any adjuvant ... B-CD8+ T Cell Interactions in the Anti-Idiotypic Response against a Self-Antibody. Darel Martínez,1 Amaury Pupo,2 Lianet ... Both humoral and CTL anti-idiotypic responses could be mechanisms to protect against the self-reactive antibody, contributing ... Interestingly, transfer of both B-1a and B-2 to BALB/Xid mice was required to recover anti-P3 IgG response in this model. In ...
"Syngeneic anti-idiotypic monoclonal antibodies to an anti-NeuGc-containing ganglioside monoclonal antibody," Hybridoma, vol. 17 ... "Establishment and characterization of an anti-idiotypic CD4+ CD8- T cell line to murine anti-alpha(1 --, 3) dextran antibody," ... B-CD8+ T Cell Interactions in the Anti-Idiotypic Response against a Self-Antibody. Darel Martínez,1 Amaury Pupo,2 Lianet ... S. Oak, L. K. Gilliam, M. Landin-Olsson et al., "The lack of anti-idiotypic antibodies, not the presence of the corresponding ...
... are leading candidates to produce anti-idiotypic antibodies. In this work, we constructed an antibody phage library from the ... Single-domain antibodies from camlid heavy-chain antibodies with the benefit features of small size, thermostability, and ease ... Anti-idiotypic antibodies recognize the antigenic determinants of an antibody, thus they can be used as surrogate antigens. ... Anti-idiotypic antibodies recognize the antigenic determinants of an antibody, thus they can be used as surrogate antigens. ...
Recently, Creative Biolabs announced the anti-idiotypic antibody for pre-clinical study of therapeutic a... ... 23, 2018, Creative Biolabs, as a world-leading supplier and manufacturer in recombinant antibody (rAb) discovery, has served ... Creative Biolabs developed anti-idiotypic antibody for assisting researchers projects. Our anti-idiotypic antibody is ... What is anti-idiotypic antibody?. Antibody is a kind of recognition proteins which are mainly found in the serum and other ...
Chain Interactions and Idiotypic Specificities of Homogeneous Rabbit Anti-Lactose Antibodies. Asoke C. Ghose and Fred Karush ... Chain Interactions and Idiotypic Specificities of Homogeneous Rabbit Anti-Lactose Antibodies Message Subject (Your Name) has ... Functionally homogeneous fractions of rabbit anti-lactose IgG antibody have been characterized with respect to their idiotypic ... This proposal would account for the inhibition by ligands as the result of the inability of ligand and anti-idiotype antibody ...
Peptidomimics of antigen are present in variable region of heavy and light chains of anti-idiotypic antibody and function as ... We have sequenced the variable regions of heavy and light chains of the anti-idiotypic antibody specific to rinderpest virus ... The functional capacity of anti-idiotypic antibody derived peptides to stimulate antigen specific T cells in vitro was tested. ... Here, we show that short peptides derived from internal image sequences of anti-idiotypic antibody (peptidomimics) can function ...
Ab2/3H6 is an anti-idiotypic antibody able to mimic the antigen recognition site of the broadly neutralizing HIV-1 antibody 2F5 ... Ab2/3H6 is an anti-idiotypic antibody able to mimic the antigen recognition site of the broadly neutralizing HIV-1 antibody 2F5 ...
... yeast killer toxin-like anti-idiotypic antibodies.. L Polonelli, F De Bernardis, S Conti, M Boccanera, M Gerloni, G Morace, W ... yeast killer toxin-like anti-idiotypic antibodies.. L Polonelli, F De Bernardis, S Conti, M Boccanera, M Gerloni, G Morace, W ... Idiotypic intravaginal vaccination to protect against candidal vaginitis by secretory, yeast killer toxin-like anti-idiotypic ... Idiotypic intravaginal vaccination to protect against candidal vaginitis by secretory, yeast killer toxin-like anti-idiotypic ...
The activation of A48 Id+ anti-BL clones anti-A48 Id antibody is specific because the pretreatment of newborn mice with anti- ... Neonatal treatment with low doses of anti-idiotypic antibody leads to the expression of a silent clone.. J Hiernaux, C Bona, P ... Neonatal treatment with low doses of anti-idiotypic antibody leads to the expression of a silent clone. ... Moreover, pretreatment of mice with anti-A48 Id antibody does not alter the MOPC 460 Id+ component of the anti-TNP response. It ...
72 by Human Anti-Idiotypic Antibodies Formed after Treatment with the Anti-Tumour-Associated Glycoprotein 72 Antibody B72.3 ...
Anti-idiotypic antibodies to anti-steroids: § 252-253 - Anti-idiotypic antibodies against anti-progesterone Description: Anti- ... Anti-idiotypic antibody raised against anti-progesterone-7- BSA.KLH conjugate (clone 2H4). Available clones: 15F11 (betatypic ... Anti-Idiotypic Antibodies Against Progesterone (No. T4-252). Lead Researcher: Dr. Fortune Kohen ... idiotypic antibody raised against anti-progesterone-7- BSA.KLH conjugate (clone 2H4). Available clones: 15F11 (betatypic), 2E11 ...
Anti-idiotypic antibody to an antibody to amyloid BETA PEPTIDE - Google Patents. Anti-idiotypic antibody to an antibody to ... monoclonal anti-idiotypic anti-Αβ antibody antibody spiked in 5% human serum) have been incubated in the wells. Anti anti-Αβ ... It has been found that by using an antibody against an antibody against the amyloid β peptide (anti-anti-Αβ antibody antibody) ... or anti-idiotypic antibodies against monoclonal anti-Αβ antibody as standard are pre-incubated with monoclonal anti-Αβ antibody ...
"Characterization of anti-idiotypic antibodies mimicking antibody- and receptor-binding sites on hepatitis A virus, Archives of ... Characterization of anti-idiotypic antibodies mimicking antibody- and receptor-binding sites on hepatitis A virus. Kiyohara, ... Characterization of anti-idiotypic antibodies mimicking antibody- and receptor-binding sites on... Kiyohara, Tomoko; Totsuka, ... Characterization of anti-idiotypic antibodies mimicking antibody- and receptor-binding sites on hepatitis A virus. ...
... anti-idotypic antibodies, anti- ids). Some of the antibodies are directed against the binding sites of the eliciting antibodies ... anti-idotypic antibodies, anti- ids). Some of the antibodies are directed against the binding sites of the eliciting antibodies ... anti-idotypic antibodies, anti- ids). Some of the antibodies are directed against the binding sites of the eliciting antibodies ... anti-idotypic antibodies, anti- ids). Some of the antibodies are directed against the binding sites of the eliciting antibodies ...
"Induction of neutralizing antibodies to papillomaviruses by anti-idiotypic antibodies",. abstract = "Anti-idiotypic antibodies ... Anti-idiotypic antibodies (anti-Ids) were generated against three mouse monoclonal antibodies (MAbs) which neutralized three ... N2 - Anti-idiotypic antibodies (anti-Ids) were generated against three mouse monoclonal antibodies (MAbs) which neutralized ... AB - Anti-idiotypic antibodies (anti-Ids) were generated against three mouse monoclonal antibodies (MAbs) which neutralized ...
A human monoclonal anti-idiotypic antibody, 105AD7, which mimics a colorectal tumor associated antigen, 791Tgp72, has been ... Clinical evidence that the human monoclonal anti-idiotypic antibody 105AD7, delays tumor growth by stimulating anti-tumor T- ... Keywords: Human monoclonal antibody, vaccine, colorectal cancer, anti-idiotypic antibody, cellular immune responses ... Abstract: A human monoclonal anti-idiotypic antibody, 105AD7, which mimics a colorectal tumor associated antigen, 791Tgp72, has ...
The five Ab2 MAbs were injected into syngeneic mice and four of them produced strong anti-anti-idiotypic antibody (Ab3) ... When this MAb was administered alone in syngeneic mice, an specific IgG anti-idiotypic antibody (Ab2) response was induced, ... While these Ab2 MAbs were unable to generate Ab3 antibodies with the same antigenic specificity than P3 MAb, three of them ... induced antibodies bearing P3 MAb idiotopes (Ag-Id+ Ab3). These results demonstrated that these Ab2 MAbs are not internal ...
Anti-Idiotypic Reagents. January 2019 Vol. 39 No. 1 GPCR Products. January 2019 Vol. 39 No. 1 ... Antibody-Antigen Products. January 2019 Vol. 39 No. 1 Electronic Pipette Filler. ...
Idiotypic Antibody to Anti‐U1‐Ribonucleoprotein Antibody",. abstract = "We produced monoclonal antiidiotypic antibody against ... T1 - Suppression of in Vitro Production of Anti‐U1‐Ribonucleoprotein Antibody by Monoclonal AntiIdiotypic Antibody to Anti‐U1‐ ... Suppression of in Vitro Production of Anti‐U1‐Ribonucleoprotein Antibody by Monoclonal AntiIdiotypic Antibody to Anti‐U1‐ ... Suppression of in Vitro Production of Anti‐U1‐Ribonucleoprotein Antibody by Monoclonal AntiIdiotypic Antibody to Anti‐U1‐ ...
Catalysis of ecterilysis by anti-idiotypic antibody towards acetylcholinesterase. E. S. Aleksandrova, F. Koralewski, M. I. ... Fingerprint Dive into the research topics of Catalysis of ecterilysis by anti-idiotypic antibody towards acetylcholinesterase ...
... "anti-idiotypic antibody". If such is the case, the anti-idiotypic antibodies will be able to bind to the B lymphocyte receptor ... An anti-idiotypic monoclonal antibody was generated to possess an "internal image of cocaine". The anti-idiotypic antibody ... Ladjemi MZ (2012). "Anti-idiotypic antibodies as cancer vaccines: achievements and future improvements". Front Oncol. 2: 158. ... Because of the resemblance of anti-idiotypic antibodies to the original antigen, vaccine studies have been performed. These ...
Anti-idiotypic antibodies are critical reagents for bioanalytical assays supporting clinical evaluation of mAb drugs; choose ... Anti-idiotypic Antibodies Anti-Adalimumab Antibodies Anti-Alemtuzumab Antibodies Anti-Bevacizumab Antibodies Anti-Cetuximab ... Anti-Panitumumab Antibodies Anti-Rituximab Antibodies Anti-Tocilizumab Antibodies Anti-Trastuzumab Antibodies Anti-Ustekinumab ... Antibodies Anti-Denosumab Antibodies Anti-Etanercept Antibodies Anti-Golimumab Antibodies Anti-Infliximab Antibodies Anti- ...
Anti-idiotypic antibody (anti-ID antibody) is a class of antibody that specifically binds to the antigen-binding site of an ... Beyond an increasing number of our anti-ID antibody products, comprehensive anti-ID antibody custom service is also available ... Creative Biolabs Announced Anti-idiotypic Antibody to Meet Scientists Research Needs. Print This Article Share it With Friends ... Creative Biolabs Announced Anti-idiotypic Antibody to Meet Scientists Research Needs. Back To Homepage Subscribe To RSS Feed ...
Induction of broadly neutralizing antibodies (bnAbs) against HIV-1 is the utmost critical goal towards the development of a ... Although the process is conceptually similar to generating PGT128-like anti-anti- idiotypic antibodies, our strategy is ... Evaluation of a Novel Antigen-Enhanced, Anti-Idiotypic Antibody Vaccine Strategy Cho, Michael W. Neovaxsyn, Inc., Ames, IA, ... Evaluation of a Novel Antigen-Enhanced, Anti-Idiotypic Antibody Vaccine Strategy. Cho, Michael W. / Neovaxsyn, Inc.. ...
Reth, M; Kelsoe, G; and Rajewsky, K, "Idiotypic regulation by isologous monoclonal anti-idiotope antibodies." (1981). Subject ...
Escalating doses of the antibody were administered to the patient in two courses of therapy. The first course did not result in ... The failure to induce an anti-tumor response in the patient was probably related to the large concentration (approximately 400 ... and the protocol was modified to consist of five doses of antibody given over a 10-day period, with the highest dose being 500 ... AbstractA monoclonal antibody (H-99) was prepared that reacts specifically with the leukemia cells from a patient with stage IV ...
  • But eventually, you're going to get rid of all the antigens **, and then you're likely to have a surplus of antibodies - now useless. (everything2.com)
  • But your body is prepared - it has already made a second set of meta-antibodies (not a technical term) to get rid of the extra antibodies by mimicking the antigens. (everything2.com)
  • To keep things running along smoothly, the production of anti-idiotypic antibodies is automatic, but blocked by the presence of the antigens. (everything2.com)
  • Both humoral and CTL anti-idiotypic responses could be mechanisms to protect against the self-reactive antibody, contributing to keeping the tolerance to self-antigens. (hindawi.com)
  • Anti-idiotypic antibodies recognize the antigenic determinants of an antibody, thus they can be used as surrogate antigens. (cdc.gov)
  • The use of biotechnology in developing the surrogate, the absence of standard aflatoxin and organic solvents in the synthesis procedures, and the reproducibility of the VHH antibody makes it an ideal strategy for replacing conventional synthesized antigens. (cdc.gov)
  • Antibody is a kind of recognition proteins which are mainly found in the serum and other bodily fluids of vertebrates that react specifically with the antigens that induced their formation. (pharmiweb.com)
  • An IgM monoclonal antibody (MAb), named P3, has the characteristic to react specifically with a broad battery of N-glycolyl containing-gangliosides and with antigens expressed on breast tumors. (semanticscholar.org)
  • The primary objective of this study is to generate mV3-antibody complexes that could be used as a primogen to induce PGT128-like bnAbs, or at least induce a large repertoire of antibodies that target the PGT128 neutralizing epitope, which could be further refined by sequential boosting with progressively more native envelope antigens. (grantome.com)
  • Kabawat SE, Bast RC, Welch WR, Knapp RC, Colvin RB (1983) Immunopathologic characterization of a monoclonal antibody that recognizes common surface antigens of human ovarian tumors of serous, endometrioid, and clear cell types. (springer.com)
  • Immunoaffinity-purified antibodies against soluble Schistosoma mansoni egg antigens (SEA) were isolated from the sera of patients with schistosomiasis mansoni. (ajtmh.org)
  • Agglutinins and antibodies to Francisella tularensis outer membrane antigens in the early diagnosis of disease during an outbreak of tularemia. (asm.org)
  • The vaccines are composed of antibodies mimicking tumor antigens (anti-idiotypic antibodies) or the antigens expressed in viruses (recombinant adeno- or vaccinia-viruses) and are selected based on their high probability of inducing both humoral and cellular immune responses in patients. (upenn.edu)
  • 1. A monoclonal antibody produced by hybridoma cell line 7E11-C5, ATCC Designation HB 10494, which monoclonal antibody binds specifically to an epitope present on a membrane associated antigen of human prostatic cancer epithelium and normal prostatic epithelium and which does not bind to non-prostatic antigens present in other tissues. (google.com)
  • This invention relates to the production of and applications for monoclonal antibodies specific for prostatic tumor antigens. (google.com)
  • Monoclonal antibodies capable of reacting with membrane associated surface antigens are of value for the immuno-classification and detection of disease and represent novel agents for immunotherapy. (google.com)
  • Immunohistochemical characterization of 30 monoclonal antibodies against cytokeratin antigens. (kau.se)
  • The major targets of the antibody response against influenza virus are the surface glycoprotein antigens hemagglutinin (HA) and neuraminidase (NA). (biomedcentral.com)
  • This antibody subset contrasts with immune antibodies, which are produced in response to the introduction of antigens to the immune system. (els.net)
  • Natural antibodies are frequently directed to intracellular structures, rather than to cell‐surface antigens. (els.net)
  • Studies of rodents rendered deficient in natural antibodies suggest that this antibody subset is endowed with a homeostatic potential and housekeeping functions, such as recognition and removal of senescent cells and other self‐antigens. (els.net)
  • Human adenovirus 2 E1B-19K and E1B-53K tumor antigens: antipeptide antibodies targeted to the NH2 and COOH termini. (asm.org)
  • abstract = "Under a variety of circumstances antibodies can be elicited against the variable region of other antibody molecules (anti-idotypic antibodies, anti- ids). (elsevier.com)
  • Krantz MJ, MacLean G, Longenecker BM, Suresh MR (1988) A radioimmunoassay for CA 125 employing two new monoclonal antibodies (Abstract). (springer.com)
  • Anti-idiotypic antibodies are critical reagents for the development of bioanalytical assays for such evaluations. (bio-rad-antibodies.com)
  • The first glycoengineered mAbs are about to enter clinical trials and it is expected that, once glycoengineering reagents are available at affordable costs, and in-line with regulatory requirements, that targeted remodelling of antibody Fc glycosylation will become an integral part in manufacturing the next-generation of immunotherapeutics. (bioportfolio.com)
  • M.R. Schick and R.C. Kennedy , Production and Characterization of Anti-idiotypic Antibody Reagents. (elsevier.com)
  • You can expect the same for your customized antibody reagents. (rndsystems.com)
  • It is suggested that the major portion of the idiotypic determinant is constituted of amino acid residues located along the periphery of the binding site rather than the contact residues in the groove or cleft of the site. (jimmunol.org)
  • To determine if the epitope for 7H11D6 is comprised of residues involved in the alpha 2M receptor determinant, anti-idiotypic antibodies against 7H11D6 were prepared. (duke.edu)
  • The results indicate either that the combining site of anti-benzoate antibody is part of an important idiotypic determinant, which is sterically blocked by hapten, or that the hapten induces a conformational change which alters idiotypic determinants not involving the active site. (rupress.org)
  • Antibodies against the idiotypic determinant ( 18 ) and against the carboxy terminus of the IgG heavy chain where IL-2 is linked (Fc-IL2 end) were detected. (aacrjournals.org)
  • Our priority is to provide the highest quality monoclonal , polyclonal , and single domain antibody services in the industry. (prosci-inc.com)
  • As for immunogenicity assays, polyclonal anti-IDs are widely applied. (pharmiweb.com)
  • The polyclonal anti-Ids were screened in an ELISA using a panel of 10 N-MAbs (4 HPV-11 neutralizing, 5 CRPV neutralizing, and 1 BPV-1 neutralizing), and cross-reactive idiotypic reactivity was observed. (elsevier.com)
  • Two of the three polyclonal rabbit anti-Ids induced strong anti-viral antibody responses (including virus-neutralizing antibodies) in BALB/c mice following immunization. (elsevier.com)
  • If you have invested in high technology platforms for discovery of your mAb drug, and appreciate the specificity, affinity and flexibility achievable with these, why revert back to animal generated monoclonal or polyclonal antibodies as research tools to support the drug's clinical evaluation? (bio-rad-antibodies.com)
  • A polyclonal anti-idiotypic antibody was produced in rabbits against the murine monoclonal antibody OSA-1 . (musc.edu)
  • Antibodies directed against FVIII have been found to be comprised of a polyclonal IgG response. (bloodjournal.org)
  • Generate custom monoclonal or polyclonal antibodies from scratch. (rndsystems.com)
  • This antibody is able to induce an anti-idiotypic IgG response and B-T idiotypic cascade, even in the absence of any adjuvant or carrier protein. (hindawi.com)
  • It is well-known that the immunogenicity is the ability of therapeutic to induce a humoral and cell-mediated immune response leading to development of anti-drug-antibodies. (pharmiweb.com)
  • As such, developing a primogen that can induce antibodies directed predominantly against the PGT128 neutralizing epitope is of paramount importance. (grantome.com)
  • The failure to induce an anti-tumor response in the patient was probably related to the large concentration (approximately 400 μg/mL) of free serum idiotype in addition to the severely compromised immune system of the patient at the time of treatment. (mdedge.com)
  • According to Jerne's network theory, a subset of anti-idiotypic antibodies (Ab2beta) form an 'internal image' of the antigen and induce antibodies (Ab3) against the original antigen. (egms.de)
  • These results indicate that lupus autoantibodies which share Ag binding properties with pathogenic Ig, including both cross-reactive and anti-dsDNA antibodies, can induce the production of Ig with similar autoantigen binding properties through immune-network interactions. (elsevier.com)
  • Circulating antiphospholipid antibodies (APL) induce vascular injury and endothelial dysfunction, which are associated with thrombotic events and/or fetal loss. (bioportfolio.com)
  • Anti-idiotypic antibodies induce neutralizing antibodies to rabies virus glycoprotein. (asm.org)
  • Anti-OJ autoantibodies: Rare or underdetected? (bioportfolio.com)
  • Anti-OJ autoantibodies are rare myositis-specific autoantibodies that have been described to target isoleucyl-tRNA synthetase. (bioportfolio.com)
  • Interestingly, 15% of patients with MG, which is caused by autoantibodies against the AChR, have serum antibodies against DEX. (rupress.org)
  • However, some of these anti-Ids would also be autoantibodies against the AChR. (rupress.org)
  • Association between anti-Sm and anti-ribosomal P protein autoantibodies in human systemic lupus erythematosus and MRL/lpr mice. (unc.edu)
  • Cellular interactions for the in vitro production of anti-chromatin autoantibodies in MRL/Mp-lpr/lpr mice. (unc.edu)
  • In vitro and in vivo effects of antiidiotypic antibodies to spontaneously occuring autoantibodies to rat thyroglobulin. (springer.com)
  • Anti-oxidized LDL (oxLDL) antibodies are exceptional among the autoantibodies prevalent in atherosclerosis: on the one hand, a correlation was found between the existence and titers of anti-oxLDL antibodies and the extent of atherosclerosis and cardiovascular disease (CVD) ( Table 1 ). (ahajournals.org)
  • B lymphocytes secreting natural antibodies could represent a reservoir capable of mutating their immunoglobulin variable region genes to give rise to high‐affinity pathogenic autoantibodies. (els.net)
  • There is accumulating clinical evidence that 105AD7 delays tumor growth by stimulating anti-tumor T-cell responses. (iospress.com)
  • This proposal is based on a scientific premise that a priming immunogen (primogen) plays a critical role in generating a B-cell repertoire that will determine antibody responses during subsequent exposures to antigenically related boosting immunogens. (grantome.com)
  • Neuroblastoma treatment with chimeric anti-disialoganglioside GD2 antibody ch14.18 showed objective anti-tumor responses. (egms.de)
  • Insight into the potential for DNA idiotypic fusion vaccines designed for patients by analysing xenogeneic anti-idiotypic antibody responses. (mendeley.com)
  • To generate a library of genes that reflect the entirety of antibody responses to hepatitis C virus (HCV) made during the 20-year course of HCV infection in a single patient (WH) whose vir. (bioportfolio.com)
  • Extensive idiotypic connectivity has been discovered between the antibodies composing the immune responses against the acetylcholine receptor (AChR) and alpha-1,3-dextran. (rupress.org)
  • The key anti-Ids that interconnect these two responses are more crossreactive, lower-affinity antibodies. (rupress.org)
  • In clinical practice, antibody responses against FVIII are commonly identified as FVIII inhibitors by using the Bethesda or Nijmegen-modified Bethesda Assay. (bloodjournal.org)
  • 9 Although these assays have provided important results, greatly contributing to our understanding of the loss of FVIII function seen in patients, FVIII inhibitors do not reflect the whole picture of FVIII-specific antibody responses. (bloodjournal.org)
  • 1998a) Enhanced B‐1 cell development, but impaired IgG antibody responses in mice deficient in secreted IgM. (els.net)
  • This has resulted in successful immune cell- or antibody-responses against glycolipids, with promising results having been obtained in clinical trials. (frontiersin.org)
  • It is well-known that there are three main types of detection based anti-ID antibodies. (pharmiweb.com)
  • Both healthy individuals and FDRs present GAD65Ab that are inhibited by anti-Id, masking them in conventional detection methods. (monash.edu)
  • Detection of anti-adalimumab antibodies in a RA responsive cohort of patients using three different techniques. (bioportfolio.com)
  • Routinely used multiplex assays perform poorly in detection of anti-OJ an. (bioportfolio.com)
  • Antidrug antibody detection for adalimumab depends on the type of assay used: an experimental approach to help clinicians interpret diagnostic data. (bioportfolio.com)
  • To compare different methods of antidrug antibody (ADA) against adalimumab detection in ankylosing spondylitis (AS) patients and the impact of ADA on adalimumab drug levels and mean ASDAS-CRP. (bioportfolio.com)
  • Detection of Bacteroides fragilis, Bacteroides thetaiotaomicron, and Bacteroides ovatus in clinical specimens by immunofluorescence with a monoclonal antibody to B. fragilis lipopolysaccharide. (asm.org)
  • The antibodies find use in diagnostic methods such as the detection of malignant cells associated with NSCLC and in therapeutic methods. (freepatentsonline.com)
  • More recently, several assay platforms for the detection of total FVIII-binding antibodies were presented which were based on classic ELISA formats, immunoblotting or bead-based formats. (bloodjournal.org)
  • Comparison of commercial enzyme immunoassay kits with plaque reduction neutralization test for detection of measles virus antibody. (asm.org)
  • Anti-idiotypic antibody as potential serodiagnostic reagent for detection of bluetongue virus infection. (asm.org)
  • Antibodies have been used for antigen detection and therapeutics, and their specificity combined with low toxicity make them a promising pharmaceutical commodity [ 1 ]. (mdpi.com)
  • From detection antibodies for immunoassays, to flow cytometry conjugates, we can provide small to large scale custom antibody conjugation with a fast turnaround and fully tested end material. (rndsystems.com)
  • 0.002) with IC measured during the second course of treatment, indicating that development of anti-idiotypic antibodies interfered with detection of circulating hu14.18-IL2. (aacrjournals.org)
  • Accordingly, antibodies specially targeted against the complementarity determining region or CDR region of another antibody, play key roles in conducting this research and in assay development. (prosci-inc.com)
  • 0001] The current invention is directed to an anti-idiotype antibody binding to an anti-Αβ antibody and an assay for detecting antibodies binding to the same or an overlapping epitope on an anti-Αβ antibody. (google.com)
  • A competitive enzyme-linked immunosorbent assay demonstrated that the anti-idiotypic IgG competed with alpha 2M-trypsin complexes for binding to 7H11D6. (duke.edu)
  • With the homologous Assay 1, involving only OC125 antibodies, false increases of CA 125 values were observed after infusion of OC125 fragments, which completely covered real CA 125. (springer.com)
  • In contrast, with Assay 2, 3 and 4, which involve no OC125 antibodies as capture antibodies, only slight false increases occurred in the presence of very high anti-OC125 antibody concentrations. (springer.com)
  • Interference was eliminated by addition of non-specific murine IgG in Assay 2 and 4, but not in Assay 1 and 3 indicating that the false increases in Assay 1 and 3 were caused by anti-idiotypic anti-OC125 antibodies. (springer.com)
  • In the presence of elevated real CA 125, with Assay 2 and to a considerably lesser degree with Assay 4, an inhibitory effect of anti-OC125 antibodies became evident leading to false decreases of CA 125 values. (springer.com)
  • Klug TL, Green PJ, Zurawski VR, Davis HM (1988) Confirmation of a false positive result in CA 125 immunoradiometric assay caused by human anti-idiotypic immunoglobulin. (springer.com)
  • Anti-adalimumab antibodies were detected in patients' sera by different techniques: bridging ELISA, reporter gene assay (RGA), and surface plasmon resonance (SPR). (bioportfolio.com)
  • Enzyme-linked immunosorbent assay of antibody to group A Streptococcus-specific C carbohydrate with trypsin-pronase-treated whole cells as antigen. (asm.org)
  • Rapid quantitation of cytomegalovirus and assay of neutralizing antibody by using monoclonal antibody to the major immediate-early viral protein. (asm.org)
  • We developed a novel epitope mapping assay that depends on competition of the serum antibodies with recombinant Fab derived from GAD65-specific monoclonal antibodies. (washington.edu)
  • As expected, there are binding antibodies with specificity to FVIII that do not neutralize the protein and, therefore, are not detectable using a Bethesda assay. (bloodjournal.org)
  • All patients developed some inhibitory activity in the binding inhibition assay designed to detect antibodies to the Fc-IL2 region of the IC. (aacrjournals.org)
  • P3 mAb: an immunogenic anti-NeuGcGM3 antibody with unusual immunoregulatory properties," Frontiers in Immunology , vol. 3, p. 94, 2012. (hindawi.com)
  • F. Stäb, F. Austrup, and E. Kölsch, "Regulation of the anti-alpha(1----3) dextran IgG antibody response of BALB/c mice by idiotype-specific T suppressor lymphocytes," Journal of Immunology , vol. 144, no. 1, pp. 53-59, 1990. (hindawi.com)
  • The anti-idiotype reaction was subject to inhibition with lactose and p-amino-phenyl-β-lactoside with some antisera, depending on the form in which the homogeneous antibody was presented for immunization. (jimmunol.org)
  • Pretreatment of newborn BALB/c mice (at 1 d of age) with 0.01-10 microgram of affinity purified BALB/c anti-A48 Id antibody followed by immunization with BL 1-2 mo later produces an anti-BL response that expresses the A48 Id. (rupress.org)
  • The activation of A48 Id+ anti-BL clones anti-A48 Id antibody is specific because the pretreatment of newborn mice with anti-MOPC 384 Id antibody, followed by immunization with BL, does not lead to its activation. (rupress.org)
  • Arresting progressive atherosclerosis by immunization with an anti-glycosaminoglycan monoclonal antibody in apolipoprotein E-deficient mice. (semanticscholar.org)
  • Immunization with the Sm nuclear antigen induces anti-Sm antibodies in normal and MRL mice. (unc.edu)
  • When you outsource generation of recombinant monoclonal anti-idiotypic antibodies to Bio-Rad you can bypass internal bottlenecks faced accessing the platforms prioritised for therapeutic drug discovery. (bio-rad-antibodies.com)
  • A panel of syngeneic monoclonal anti-idiotypic antibodies was prepared by immunizing A.SW mice with keyhole limpet hemocyanin-coupled A.SW monoclonal anti-myoglobin (HAL 19, IgG1) and screening the cloned hybridomas for production of IgG2 binding to idiotype but not to certain other anti-myoglobin antibodies of the same subclass in an ELISA. (elsevier.com)
  • The development of a panel of syngeneic monoclonal anti-idiotypic antibodies to different clusters of idiotopes on the same antibody molecule, including one that identifies a major common idiotope in immune sera, should allow the analysis of possible idiotype network regulation in vivo and in vitro in a completely syngeneic system. (elsevier.com)
  • The first is an antigen which is blocking anti-ID antibody, the second is named as non blocking because the antibody drug's paratope and idiotope do not overlap, the third one is a complex specific anti-ID which cannot bind to the antibody drug unless the drug is already bound to its antigen. (pharmiweb.com)
  • A monoclonal antibody against NeuGc-containing gangliosides contains a regulatory idiotope involved in the interaction with B and T cells. (semanticscholar.org)
  • For anti-idiotypic antibody projects, selection of the antibody is carried out on the drug in the presence of isotype sub-class matched antibodies as blockers, to avoid enrichment of specificities that bind to other regions of the antibody drug and to ensure idiotope specificity. (bio-rad-antibodies.com)
  • Idiotypic regulation by isologous monoclonal anti-idiotope antibodies. (jax.org)
  • Only Cluster II antibodies block the binding of myoglobin to HAL 19 and so identify a binding site-related idiotope(s). (elsevier.com)
  • Binding of both Cluster II monoclonals (S5 and S7) to Hal 19 is inhibited by a rabbit anti-idiotype that we previously reported detects a common cross-reactive anti-myoglobin idiotope in immune sera. (elsevier.com)
  • However, only one of these, S7, and not S5, identifies an idiotope that is present on 20 to 30% of A.SW anti-myoglobin antibodies in immune sera and ascites. (elsevier.com)
  • An idiotope is the unique set of antigenic determinants (epitopes) of the variable portion of an antibody. (wikipedia.org)
  • For an epitope that is sequence-specific (anti-FIPV system), molecular mimicry appears to be present as evidenced by the sequence homology between the CDR loops of the anti-id and the epitope of the original antigen. (elsevier.com)
  • O'Brien TJ, Raymond LM, Bannon GA, Ford DH, Hardardottir H, Miller FC, Quirk JG (1991) New monoclonal antibodies identify the glycoprotein carrying the CA 125 epitope. (springer.com)
  • 36. An antibody which specifically binds to an Hsp47 polypeptide epitope comprising the sequence AX 1 X 2 X 3 AEQLR (SEQ ID NO:29). (freepatentsonline.com)
  • G.F. Rimmelzwaan, E.J. Bunschoten, F.G.C.M. UytdeHaag, and A.D.M.E. Osterhaus , Monoclonal Anti-idiotypic Antibody Vaccines against Poliovirus, Canine Parvovirus, and Rabies Virus. (elsevier.com)
  • Anti-idiotypic antibodies Recently we identified the presence of anti-idiotypic antibodies directed against GAD65Ab in the majority of healthy individuals. (washington.edu)
  • For example, the high sensitivity and specificity of our antibodies allow for superior differentiation supporting the design and development of robust pharmacokinetic, immune response assays. (prosci-inc.com)
  • BALB/c mice immunized with bacterial levan (BL) produce an immune response that fails to generate antibody expressing the idiotype (Id) of the beta (2 leads to 6) fructosan-binding myeloma protein ABPC 48 (A48). (rupress.org)
  • Although the process is conceptually similar to generating PGT128-like anti-anti- idiotypic antibodies, our strategy is technically distinct and superior in that we are using an antigen-antibody immune complex as an immunogen. (grantome.com)
  • Antibodies that can neutralize (block) HIV-1 infection are important components of the body's immune system. (grantome.com)
  • The major goal of this proposal is to generate a novel vaccine candidate that can train the body's immune system to produce antibodies that can block fusion of viral and cellular membranes. (grantome.com)
  • Regulatory effects of isologous anti-idiotypic antibodies on the formation of different immunoglobulin classes in the immune response to phosphorylcholine in balb/c mice. (jax.org)
  • Molecular characterization of the anti-idiotypic immune response of a relapse-free neuroblastoma patient following antibody therapy. (egms.de)
  • If such is the case, the anti-idiotypic antibodies will be able to bind to the B lymphocyte receptor for the original antigen and inhibit the immune response to that antigen. (wikipedia.org)
  • Anti-idiotypic antibodies have been described as a regulatory element in the humoral immune response. (washington.edu)
  • T. Sasaki, Y. Koide, and K. Yoshigaga , Immune Suppression on Anti-DNA Antibody Production Using Anti-idiotypic Antibody-Neocarzinostatin Conjugates. (elsevier.com)
  • The prevalence of FVIII-binding antibodies was 34% (5% for titers ≥ 1:80) in patients without FVIII inhibitors (n = 77), 39% (4% for titers ≥ 1:80) in patients after successful immune tolerance induction therapy (n = 23), and 100% (n = 20, all titers ≥ 1:80) in patients with FVIII inhibitors. (bloodjournal.org)
  • With time, secretion of affinity‐mature, somatically selected antibodies will provide the immune system with a second wave of potent adaptive defence mechanism. (els.net)
  • Although well established, this technology is laborious, and it is biased by the experimental animal immune system, which limits the ability to reach a high-affinity antibody against conserved mammal proteins. (mdpi.com)
  • An antibody against the Fc-IL2 end of the IC (anti-Fc-IL2) might interfere with immune activation facilitated through IL-2. (aacrjournals.org)
  • The human or humanized nature of the majority of therapeutic monoclonal antibodies is particularly conducive to research with anti-idiotypic antibodies. (prosci-inc.com)
  • Recently, Creative Biolabs announced the anti-idiotypic antibody for pre-clinical study of therapeutic antibody and vaccine development. (pharmiweb.com)
  • Secondly, our anti-idiotypic antibody allows researchers to monitor the therapeutic Abs in samples. (pharmiweb.com)
  • Antibodies of this kind have been produced and used in a variety of situations including attempts at using them as therapeutic agents. (elsevier.com)
  • To meet the need in pre-clinical study of therapeutic antibody and vaccine development, Creative Biolabs expanded its antibody category and launched anti-idiotypic antibody products for various applications, such as pharmacokinetic study, neutralizing antibody study and immunogenicity study. (massachusettschronicle.com)
  • Commonly, in currently marketed therapeutic mAbs, the glycosylation profile is suboptimal in terms of biological properties such as antibody-dependent cell-mediated cytotoxicity (ADCC) or may give rise to safety concerns due to the presence of non-human glycans.This article will review recent innovative developments in chemo-enzymatic glycoengineering, which allow generating mAbs carrying single, well-defined, uniform Fc glycoforms, which confers the desired biological properties for the target application. (bioportfolio.com)
  • Therapeutic Monoclonal Antibodies to Complex Membrane Protein Targets: Antigen Generation and Antibody Discovery Strategies. (bioportfolio.com)
  • Engineering therapeutic bispecific antibodies using CrossMab technology. (bioportfolio.com)
  • Bispecific antibodies have recently gained major interest as they allow novel mechanisms-of-action and/or therapeutic applications that cannot be achieved using conventional IgG-based antibodies. (bioportfolio.com)
  • The study gives a unique perspective into this therapeutic strategy because the three other anti-CD38 cytolytic antibodies in clinical development (daratumumab, isatuximab, and MOR202) cannot be tested in similar models because they do not crossreact with CD38 expressed by new world primates. (aspetjournals.org)
  • Therapeutic use of antibodies is limited by methodological constraints in raising them. (mdpi.com)
  • Additionally, the heterologous character of those proteins turn them often immunogenic to humans eliciting HAMA response (Human Anti-Mouse Antibodies), which restrict their therapeutic use [ 5 ]. (mdpi.com)
  • Anti- idiotypic antibody (anti-ID antibody) is a class of antibody that specifically binds to the antigen-binding site of an antibody. (massachusettschronicle.com)
  • In some cases it can be the actual antigen-binding site, and in some cases it may comprise variable region sequences outside of the antigen-binding site on the antibody itself. (wikipedia.org)
  • TAK-079 is a human monoclonal antibody (IgG 1 λ ) that binds to CD38 and lyses bound cells by complement-dependent cytotoxicity and antibody-dependent cell-mediated cytotoxicity. (aspetjournals.org)
  • The ability to readily distinguish between endogenous antibodies in a patient's blood serum and antibody drugs is critical and made possible through the introduction of anti-idiotype antibodies which bind the unique idiotopes of each unique antibody drug. (prosci-inc.com)
  • Normally, the high concentration of antibodies found in the serum of a patient receiving a human or humanized antibody drug tends to crowd out the drug of interest, rendering it practically invisible, and thus very challenging to track and analyze. (prosci-inc.com)
  • To clone anti-idiotypic antibodies, B-cells of a ch14.18-treated neuroblastoma patient with Ab2 serum reactivity were used to construct antibody phage display libraries (Methods described in Arthritis Rheum. (egms.de)
  • Intriguingly, they were able to compete with natural patient's antibodies, contained in his serum, for binding to 14G2a. (egms.de)
  • Notably, in each serum the autoantibody activity was confined to the anti-Id Ig fraction. (elsevier.com)
  • The purpose of this prospective cohort study is to evaluate the influence of serum drug levels and development of anti-drug antibodies on clinical response to anti-TNF agents in ankylosing. (bioportfolio.com)
  • All patients had detectable anti-C1 inhibitor antibodies in serum. (biomedsearch.com)
  • Phage display represents a high-throughput technique for screening billions of random fusion antibodies against virtually any target on the surface or inside cancer cells, or even soluble markers found in patient serum. (mdpi.com)
  • Serum was assayed for anti-idiotypic antibody (anti-id Ab) based on ability to bridge biotinylated hu14.18 to plate-bound hu14.18 and ability to inhibit binding of hu14.18 to GD2 antigen and/or murine anti-idiotypic antibody. (aacrjournals.org)
  • There was a positive correlation between the peak serum level of IC in course 1 and the anti-Fc-IL2 response. (aacrjournals.org)
  • The specificity of this special region has emerged as a significant area of interest, particularly in research involving antibody drug pharmacodynamics, studies in immunogenicity and drug pharmacokinetics. (prosci-inc.com)
  • Our anti-idiotypic antibody is suitable for a variety of applications, including pharmacokinetic study, immunogenicity study and neutralizing antibody study. (pharmiweb.com)
  • In addition to the pharmacokinetic assays, anti-idiotypic antibody from Creative Biolabs is also a remarkable tool in immunogenicity assays. (pharmiweb.com)
  • It plays a very crucial role in analyzing the immunogenicity of a new antibody drug during preclinical analysis. (pharmiweb.com)
  • The immunogenicity of anti-tumor necrosis factor alpha (anti-TNF) therapy in inflammatory bowel disease (IBD) is an important cause of loss of response to therapy that may lead to escalati. (bioportfolio.com)
  • Human antibodies are of particular interest due to a lower immunogenicity response [ 1 ]. (mdpi.com)
  • Studies were performed to determine if monoclonal antibody 7H11D6 binds to the region of alpha 2-macroglobulin (alpha 2M) that interacts with cell-surface receptors. (duke.edu)
  • A.D. Strosberg , Interaction of Anti-idiotypic Antibodies with Membrane Receptors: Practical Considerations. (elsevier.com)
  • Natural antibodies could serve as innate recognition receptors, recognising various bacterial cell‐wall components or parasites. (els.net)
  • Cell receptors for baboon endogenous virus recognized by monoclonal antibodies. (asm.org)
  • The invention provides methods for production of the monoclonal antibodies by hybridoma techniques. (google.com)
  • Immunotherapy with unmodified anti-Id antibody alone does not reveal these variants, and we suggest it is the increased selective force exerted by the highly potent immunotoxins that allow these minor nonreactive populations to emerge. (elsevier.com)
  • Despite the recent success of passive immunotherapy with the anti-GD2 antibody ch14.18 and cytokines , treatment of high-risk NB remains challenging. (curehunter.com)
  • We expanded the approach of GD2-specific, antibody-based immunotherapy to an application of a GD2-specific natural killer (NK) cell line, NK-92-scFv(ch14.18)-zeta. (curehunter.com)
  • Monoclonal antibodies (mAb) that block programmed death (PD)-1 signaling pathway hold great potential as a novel cancer immunotherapy. (biomedcentral.com)
  • Once cloned, cell lines can be maintained continuously to produce an unlimited homogeneous monoclonal antibody population that can be isolated and/or purified and used clinically for in vitro immunohistological, immuno-cytological or immuno-serological diagnosis, in vivo diagnosis by localization of tumors and metastases, and immunotherapy of human cancers, particularly those of the prostate. (google.com)
  • Generation of a murine monoclonal antibody specific for N-glycolylneuraminic acid-containing gangliosides that also recognizes sulfated glycolipids," Hybridoma , vol. 14, no. 6, pp. 551-556, 1995. (hindawi.com)
  • Immunogenetic analysis of variable regions encoding AB1 and gamma-type AB2 antibodies from the NeuGc-containing ganglioside family," Hybridoma , vol. 20, no. 4, pp. 211-221, 2001. (hindawi.com)
  • Syngeneic anti-idiotypic monoclonal antibodies to an anti-NeuGc-containing ganglioside monoclonal antibody," Hybridoma , vol. 17, no. 6, pp. 527-534, 1998. (hindawi.com)
  • 1, 1986, filed Mar. 11, 1983 entitled: Monoclonal Antibodies to Human Gastrointestinal Cancers and Hybridoma Method of Produciton of the Monoclonal Antibodies, by Sakamoto et al. (freepatentsonline.com)
  • Monoclonal antibodies to prostatic cells, are produced by a hybridoma formed by fusing mouse lymphocytes and mouse myeloma cells. (google.com)
  • The screening leads to a specific antibody-producing hybridoma [ 4 ]. (mdpi.com)
  • Nov. 23, 2018, Creative Biolabs, as a world-leading supplier and manufacturer in recombinant antibody (rAb) discovery, has served customers in academia and industry fields with high-quality services and products. (pharmiweb.com)
  • Over the last 10 years, Creative Biolabs has been a leader of recombinant antibody (rAb) discovery and manufacturing, providing high quality service to customers in academia and industry fields all over the world. (massachusettschronicle.com)
  • Develop a new custom recombinant antibody or enhance your existing clones. (rndsystems.com)
  • We are dedicated to ensuring our custom antibody solution fits the customer's needs by utilizing cutting edge protocols and incorporating recombinant antibody technology and antibody engineering. (rndsystems.com)
  • The best immunoassay developed with one of these VHH antibodies shows an IC50 of 0.16 ng/mL toward aflatoxin B1 and cross-reactivity toward aflatoxin B2, G1, and G2 of 90.4%, 54.4%, and 37.7%, respectively. (cdc.gov)
  • Affinity-purified anti-B1.A1 (BPV-1 N-MAb) also showed unexpected cross-reactivity with the 4 HPV-11 N-MAbs, but not with any of the CRPV N-MAbs. (elsevier.com)
  • Certain anti-DEX antibodies also bind to anti-AChR antibodies via idiotypic interactions. (rupress.org)
  • 4 ⇓ - 6 Interactions between B cells and CD4 + T cells not only initiate expansion and differentiation of B cells, but also trigger isotype switching and affinity maturation of antibodies. (bloodjournal.org)
  • The presence of GAD65Ab-specific anti-Ids was confirmed by competitive ELISA. (monash.edu)
  • Summarizing, ELISA, RGA and SPR recognized anti-adalimumab antibodies in few RA patients, showing good agreement among the methodology employed. (bioportfolio.com)
  • On the other hand, differences observed between SPR and ELISA or RGA highlight the relevance of the employed technologies in anti-drug antibody identification. (bioportfolio.com)
  • Patient sera were examined in ELISA to determine if an anti-IC antibody response occurred during treatment. (aacrjournals.org)
  • ELISA was also used to detect antibodies to the Fc-IL2 end of hu14.18-IL2. (aacrjournals.org)
  • The development of antibody to hu14.18-IL2 detected in the bridge ELISA was not related to the dose of hu14.18-IL2. (aacrjournals.org)
  • Twenty of 33 adult patients (61%) demonstrated an anti-idiotypic antibody response based on binding inhibition ELISA. (aacrjournals.org)
  • The specificity of monoclonal anti‐idiotypic antibody (IgG1, x) was determined by inhibition studies. (elsevier.com)
  • The idiotypic region of an antibody is defined as the unique set of antigenic determinants located in its variable region. (prosci-inc.com)
  • Functionally homogeneous fractions of rabbit anti-lactose IgG antibody have been characterized with respect to their idiotypic specificity and the effect of homologous recombination of isolated chains on the recovery of functional homogeneity and idiotypic determinants. (jimmunol.org)
  • C. Petit, M.E. Sauron, M. Gilbert, and J. Thabeze , Use of Immunoblotting to Detect Idiotypic Determinants on Monoclonal Antibodies. (elsevier.com)
  • More particularly, this invention relates to monoclonal antibodies against non-soluble, membrane associated, organ specific determinants expressed maximally on human normal and neoplastic prostatic epithelium. (google.com)
  • Such conformational changes, if they occur, must be restricted since hapten has little effect on the reactions of F(ab') 2 fragments of anti-benzoate antibodies with antisera directed to rabbit fragment Fab and no detectable effect on reactions with antibodies directed to allotypic determinants. (rupress.org)
  • Firstly, it is an ideal tool for pre-clinical research and antibody drug development studies. (pharmiweb.com)
  • You receive the highly specific antibodies you need in a matter of weeks, rather than months, and have a long-term secure supply to support clinical evaluation of your drug. (bio-rad-antibodies.com)
  • Neuronal surface antigen antibodies in limbic encephalitis: clinical-immunologic associations. (medscape.com)
  • Dalakas MC, Fujii M, Li M, McElroy B. The clinical spectrum of anti-GAD antibody-positive patients with stiff-person syndrome. (medscape.com)
  • Reliable monitoring of clinical relevant anti-drug antibodies is fundamental in the follow-up of patients under adalimumab treatment. (bioportfolio.com)
  • Diffuse pruritic erythema as a clinical manifestation in anti-SAE antibody-associated dermatomyositis: a case report and literature review. (bioportfolio.com)
  • We evaluated the clinical performance of anti-CEP-1 in a Chinese rheumatoid arthritis (RA) cohort. (bioportfolio.com)
  • The monoclonal antibodies of this invention possess distinctive characteristics and capabilities which make them suitable for in vitro clinical diagnostic and prognostic purposes. (google.com)
  • Examples of such antibodies will be discussed here with emphasis on those used as probes for molecular imaging and other clinical trials. (mdpi.com)
  • No association was seen between development of anti-IC antibodies and clinical toxicity. (aacrjournals.org)
  • The discovery that some of these patients develop antibodies to the hu14.18-IL2 molecule will help us in the design of future clinical trials with this molecule. (aacrjournals.org)
  • The anti‐idiotypic antibody specifically suppressed the in vitro production of anti‐U1‐RNP antibody by lymphocytes from the patient K T., and unrelated patients with a cross‐reactive idiotype, in whom idiotype‐reactive T cells were demonstrated. (elsevier.com)
  • Because of the resemblance of anti-idiotypic antibodies to the original antigen, vaccine studies have been performed. (wikipedia.org)
  • Of course, anti-idiotypic antibody is also useful in vaccine development. (pharmiweb.com)
  • Ab2/3H6 is an anti-idiotypic antibody able to mimic the antigen recognition site of the broadly neutralizing HIV-1 antibody 2F5, making it a putative candidate as an HIV-1 vaccine. (moleculardevices.com)
  • To this aim, a murine mAb (KT4, IgG1) neutralizing in vitro the anti-Candida activity of a yeast killer toxin (YKT) was used as an Id vaccine to elicit Abs with toxin-like activity in a rat vaginitis model. (jimmunol.org)
  • Induction of broadly neutralizing antibodies (bnAbs) against HIV-1 is the utmost critical goal towards the development of a protective AIDS vaccine. (grantome.com)
  • The critical roadblock to AIDS vaccine development is the difficulty in eliciting neutralizing antibodies that are broadly reactive against many different variants of the virus. (grantome.com)
  • Thus we cloned human anti-idiotypic Fab which may be suitable as a tumor vaccine against GD2-positive tumors. (egms.de)
  • I. European patent application No. 99 914 725.9 published as International application No. WO 99/54457 with the title 'Vaccine formulations comprising antiidiotypic antibodies which immunologically mimic group B streptococcal carbohydrates' was refused by the examining division. (epo.org)
  • Given the conserved nature of the stem region, stem-based vaccine approaches aim to elicit antibodies that recognize both homosubtypic and heterosubtypic strains. (biomedcentral.com)
  • In this context, vaccine approaches aimed at eliciting antibodies targeting the influenza HA glycoprotein are the primary focus. (biomedcentral.com)
  • The protection was associated with rising vaginal titers of anti-idiotypic Abs (IdAb), prevalently of the IgA isotype, that were able to passively transfer the protective state to nonimmunized animals. (jimmunol.org)
  • Importantly anti-idiotypic antibody titers are elevated during remission in patients with autoimmune disease. (washington.edu)
  • Idiotypic network connectivity and a possible cause of myasthenia gravis. (rupress.org)
  • M.C. Souroujon and S. Fuchs , Anti-idiotypic Antibodies in the Study and Treatment of Experimental Autoimmune Myasthenia Gravis. (elsevier.com)
  • A monoclonal gammopathy was detected in 12 patients (63%) at the time of diagnosis, 11 of whom had an immunoglobulin peak of the same heavy- and light-chain isotypes as the acquired anti-C1 inhibitor antibody. (biomedsearch.com)
  • Evaluation of risk and diagnostic value of quantitative assays for anti-Toxoplasma gondii immunoglobulin A (IgA), IgE, and IgM and analytical study of specific IgG in immunodeficient patients. (asm.org)
  • Natural antibodies are essentially antibodies of the immunoglobulin M (IgM) isotype present in the circulation of normal humans and other mammalian species. (els.net)
  • 2000) B‐1 and B‐2 cell‐derived immunoglobulin M antibodies are nonredundant components of the protective response to influenza virus infection. (els.net)
  • Use of anti-idiotypic antibodies to establish that monoclonal antibody 7H11D6 binds to the alpha 2-macroglobulin receptor recognition site. (duke.edu)
  • Switching on cytotoxicity by a single mutation at the heavy chain variable region of an anti-ganglioside antibody. (semanticscholar.org)
  • Antibodies against glycolipids and globular glycoproteins are found to mediate complement-dependent cytotoxicity (CDC) because they extend less than 100 angstroms from the cell membrane while antibodies to mucins that extend up to 5000 angstroms from the cell surface do not ( 8 ). (frontiersin.org)
  • Natural killer cells mediate cytolytic activity via antibody-dependent cellular cytotoxicity and non-MHC-restricted cytotoxicity ( 9 ). (aacrjournals.org)
  • Isolation of alpaca anti-idiotypic heavy-chain single-domain antibody for the aflatoxin immunoassay. (cdc.gov)
  • We produced monoclonal anti‐idiotypic antibody against immunoaffinity purified anti‐U1‐ribonucleoprotein (RNP) antibody from a patient (K.T.), by the cell fusion procedure. (elsevier.com)
  • sera or by immunoaffinity-purified antibody preparations, substantiated that anti-SEA immunoaffinity-purified antibodies only reacted with components of SEA, and anti-epimastigote immunoaffinity-purified antibodies only reacted with components of epimastigote antigenic preparation. (ajtmh.org)
  • Interestingly, transfer of both B-1a and B-2 to BALB/Xid mice was required to recover anti-P3 IgG response in this model. (hindawi.com)
  • named 94-2 and 94-7), were generated from a BALB/c mouse immunized with human monoclonal anti-hepatitis A virus (HAV) neutralizing antibody KF94. (deepdyve.com)
  • The results indicate that within a mouse strain, papillomavirus-neutralizing antibodies share cross-reactive idiotopes that group the N-MAbs into several subsets. (elsevier.com)
  • The panel of syngeneic monoclonal anti-idiotype antibodies also detects new idiotopes not detected by the rabbit anti-idiotype. (elsevier.com)
  • and the set of these individual idiotopes is termed the idiotype of the antibody. (wikipedia.org)
  • Muto MG, Lepisto EM, Van den Abbeele AD, Knapp RC, Kassis AI (1989) Influence of human antimurine antibody on CA 125 levels in patients with ovarian cancer undergoing radioimmunotherapy or immunoscintigraphy with murine monoclonal antibody OC 125. (springer.com)
  • Here, we show that short peptides derived from internal image sequences of anti-idiotypic antibody (peptidomimics) can function as both B and T cell epitopes and perpetuate antigen specific immunological memory. (nih.gov)
  • The functional capacity of anti-idiotypic antibody derived peptides to stimulate antigen specific T cells in vitro was tested. (nih.gov)
  • J.E. Blalock, J.N. Whitaker, E.N. Benveniste, and K.L. Bost , Use of Peptides Encoded by Complimentary RNA for Generating Anti-idiotypic Antibodies of Predefined Specificity. (elsevier.com)
  • Anti-idiotypic antibodies (anti-Ids) were generated against three mouse monoclonal antibodies (MAbs) which neutralized three different papillomaviruses. (elsevier.com)
  • Affinity-purified anti-H11.B2 (HPV-11 N-MAb) cross-reacted with the 3 other HPV-11 N-MAbs, was unreactive to a nonneutralizing HPV-11 MAb and all 5 CRPV N-MAbs, but surprisingly was reactive to the BPV-1 N-MAb. (elsevier.com)
  • in contrast, affinity-purified anti-CRPV-1A (CRPV N-MAb) reacted with all 5 CRPV N-MAbs, but not with any other MAb. (elsevier.com)
  • Sera were applied to affinity columns coated with GAD65-specific mAbs to absorb anti-idiotypic antibodies (anti-Ids). (monash.edu)
  • Moreover, gangliosides are actively released from the membrane of tumor cells, having a strong impact on impairing anti-tumor immunity. (frontiersin.org)
  • The isolated chains were also evaluated with respect to binding affinity and inhibition of anti-idiotype antibody. (jimmunol.org)
  • The heavy chain was also found to inhibit slightly the anti-idiotype reaction. (jimmunol.org)
  • This proposal would account for the inhibition by ligands as the result of the inability of ligand and anti-idiotype antibody to be accommodated simultaneously at contiguous sites because of overlapping volumes. (jimmunol.org)
  • The anti-idiotypic IgG inhibited the binding of 125I-labeled alpha 2M-trypsin complexes to normal rat kidney fibroblasts, and a Kd of 194 pM for the binding of the anti-idiotype to these cells was derived from a fit of the data to a model involving a single class of sites. (duke.edu)
  • K.H.Roux , Immunoelectron Microscopy of Idiotype-Anti-idiotype Complexes. (elsevier.com)
  • M.J. Nelles , Competitive Idiotype-Anti-idiotype Enzyme Immunoassay for Adenosine Deaminase Binding Protein. (elsevier.com)
  • J-M. Grzych, F. Roussel-Velge, and A. Capron , Use of Anti-idiotype Antibodies in Approaches to Immunoprophylaxis of Schistosomiasis. (elsevier.com)
  • Nature of IgG anti-lymphocyte autoantibody-reactive molecules shed from activated T cells in systemic lupus erythematosus. (unc.edu)
  • Control sera are negative for anti-DEX antibodies. (rupress.org)
  • K.B. Raines, J.R. Baker, Y.G.Lukes, L. Wartofsky, K.D. Burman (1985) Antithyrotropin antibodies in the sera of Graves′ di sease patients. (springer.com)
  • Paschke R., Teuber J., Schwedes U., Usadel K.H. (1987) Antiidiotypic Blocking of Graves′ Disease Biologic Activity with Autologous Sera But Not Consistently with Homologous Sera: Evidence for Polyclonality of Thyroid Receptor Antibodies (Trab). (springer.com)
  • Robust techniques combining an antibody library displayed on the phage surface and protein microarray allowed the identification of auto antibodies recognized by patient sera. (mdpi.com)
  • E. Hurwitz and J. Haimovich , Use of Anti-idiotypic Antibody-Drug Conjugates to Treat Experimental Murine B-Cell Tumors. (elsevier.com)
  • We have sequenced the variable regions of heavy and light chains of the anti-idiotypic antibody specific to rinderpest virus hemagglutinin protein and predicted T cell epitopes in these sequences by an immuno-informatics approach. (nih.gov)
  • Of particular interest are the antibodies that recognize epitopes of the original antibody that are in contact with antigen. (elsevier.com)
  • Remarkably, T1D patients (n = 54) and Stiff Person Syndrome patients (n = 8) show a specific lack of anti-Ids to disease-associated GAD65Ab epitopes. (monash.edu)
  • We also succeeded to detect antibody maturation towards these epitopes during progression of disease. (washington.edu)
  • These findings suggest a model for the initiation of autoimmunity in MG. Antibodies made in response to DEX epitopes on the surface of certain bacteria would elicit the production of anti-Ids. (rupress.org)
  • These studies provided structural basis for molecular mimicry of anti-ids. (elsevier.com)
  • H. Kadohler, S. Kaveri, T. Kieber-Emmons, W.J.W. Morrow, S. Maduller, and S. Raychaudhuri , Idiotypic Networks and the Nature of Molecular Mimicry: An Overview. (elsevier.com)
  • This shows that A48 Id+ anti-BL clones belong to a normally silent fraction of the anti-BL repertoire. (rupress.org)
  • Both anti-idiotypic clones inhibited binding of ch14.18 to tumor associated antigen GD2. (egms.de)
  • 1. A monoclonal anti-idiotypic antibody specific to a human IgG 1 type monoclonal antibody possessing specificity to a nicotinic acetylcholine receptor, said monoclonal anti-idiotypic antibody being produced by the cells of a strain selected from the group consisting of TA-1 strain (FERM BP-1793), TA-2 strain (FERM BP-1794), TA-3 strain (FERM BP-1795), and TA-5 strain (FERM BP-1796). (google.es)
  • ii) detecting said antibody to a nicotinic acetylcholine receptor. (google.es)
  • MAb2 94-7 competed with an anti-HAV cellular receptor antibody for binding to HAV-susceptible cells and partially blocked virus infection. (deepdyve.com)
  • The ability to generate mAb2 implies that HAV receptor-binding sites are exposed on the surface of HAV, permitting antibody access. (deepdyve.com)
  • Binding of the anti-idiotypic IgG to the alpha 2M receptor provides unequivocal evidence that 7H11D6 is binding to residues within the receptor recognition site on alpha 2M and not merely to residues sufficiently close to that region to cause steric hindrance. (duke.edu)
  • Preparation and characterization of an antibody antagonist that targets the porcine growth hormone receptor. (thefreelibrary.com)
  • Rapid T cell receptor modulation accompanies lack of in vitro mitogenic responsiveness of double negative T cells to anti-CD3 monoclonal antibody in MRL/Mp-lpr mice. (unc.edu)
  • N.R. Farid , Anti-idiotypic Antibodies as Probes of Hormone Receptor Structure and Function. (elsevier.com)
  • J.A. Glasel , Production and Properties of Antimorphine Anti-idiotypic Antibodies and their Antiopiate Receptor Activity. (elsevier.com)
  • C. Gramsch, R. Schulz, S. Kosin, A.H.S. Hassan, and A. Herz , Production and Characterization of Anti-idiotypic Antiopioid Receptor Antibodies. (elsevier.com)
  • A.D. Strosberg , Anti-idiotypic Antibodies That Interact with ~gb-Adrenergic Catecholamine Receptor. (elsevier.com)
  • I.R. Cohen, D. Elias, M. Rapoport, and Y. Shechter , Production and Properties of Anti-idiotypic Antibodies That Recognize Insulin Receptor. (elsevier.com)
  • M.B.H. Youdim, R.J. Barkey, T. Amit, and J. Guy , Anti-idiotypic Antibodies as Probes of Prolactin Receptor. (elsevier.com)
  • W.V. Williams, D.B. Weiner, and M.I. Greene , Development and Use of Antireceptor Antibodies to Study Interaction of Mammalian Reovirus Type 3 with Its Cell Surface Receptor. (elsevier.com)
  • The globular head of HA contains the receptor binding site and antibodies targeting this region inhibit virus binding to target cells. (biomedcentral.com)
  • Due to their high specificity and sensitivity these antibodies may be applicable for research, diagnosis and therapeutics. (yedarnd.com)
  • The most significant observation was the recovery of idiotypic specificity in the homologous recombinants as well as binding affinity and functional homogeneity. (jimmunol.org)
  • Syngeneic anti-idiotypic monoclonal antibodies to an anti-NeuGc-containing ganglioside monoclonal antibody. (semanticscholar.org)