An enzyme that catalyzes the formation of anthranilate (o-aminobenzoate) and pyruvic acid from chorismate and glutamine. Anthranilate is the biosynthetic precursor of tryptophan and numerous secondary metabolites, including inducible plant defense compounds. EC 4.1.3.27.
Benzoic acids, salts, or esters that contain an amino group attached to carbon number 2 or 6 of the benzene ring structure.
An enzyme that catalyzes the formation of N-5'-phosphoribosylanthranilic acid from anthranilate and phosphoribosylpyrophosphate, the first step in tryptophan synthesis in E. coli. It exists in a complex with ANTHRANILATE SYNTHASE in bacteria. EC 2.4.2.18.
An essential amino acid that is necessary for normal growth in infants and for NITROGEN balance in adults. It is a precursor of INDOLE ALKALOIDS in plants. It is a precursor of SEROTONIN (hence its use as an antidepressant and sleep aid). It can be a precursor to NIACIN, albeit inefficiently, in mammals.
An enzyme in the tryptophan biosynthetic pathway. EC 4.1.1.48.
A cyclohexadiene carboxylic acid derived from SHIKIMIC ACID and a precursor for the biosynthesis of UBIQUINONE and the AROMATIC AMINO ACIDS.
A subclass of enzymes of the transferase class that catalyze the transfer of an amino group from a donor (generally an amino acid) to an acceptor (generally a 2-keto acid). Most of these enzymes are pyridoxyl phosphate proteins. (Dorland, 28th ed) EC 2.6.1.
An enzyme that catalyzes the conversion of L-serine and 1-(indol-3-yl)glycerol 3-phosphate to L-tryptophan and glyceraldehyde 3-phosphate. It is a pyridoxal phosphate protein that also catalyzes the conversion of serine and indole into tryptophan and water and of indoleglycerol phosphate into indole and glyceraldehyde phosphate. (From Enzyme Nomenclature, 1992) EC 4.2.1.20.
Enzymes that catalyze the transfer of nitrogenous groups, primarily amino groups, from a donor, generally an amino acid, to an acceptor, usually a 2-oxoacid. EC 2.6.
A non-essential amino acid present abundantly throughout the body and is involved in many metabolic processes. It is synthesized from GLUTAMIC ACID and AMMONIA. It is the principal carrier of NITROGEN in the body and is an important energy source for many cells.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria found in soil, water, food, and clinical specimens. It is a prominent opportunistic pathogen for hospitalized patients.
A mechanism of communication within a system in that the input signal generates an output response which returns to influence the continued activity or productivity of that system.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
The interference in synthesis of an enzyme due to the elevated level of an effector substance, usually a metabolite, whose presence would cause depression of the gene responsible for enzyme synthesis.
A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A colorless alkaline gas. It is formed in the body during decomposition of organic materials during a large number of metabolically important reactions. Note that the aqueous form of ammonia is referred to as AMMONIUM HYDROXIDE.
The functional hereditary units of BACTERIA.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.
In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.
A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Change brought about to an organisms genetic composition by unidirectional transfer (TRANSFECTION; TRANSDUCTION, GENETIC; CONJUGATION, GENETIC, etc.) and incorporation of foreign DNA into prokaryotic or eukaryotic cells by recombination of part or all of that DNA into the cell's genome.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
Enzyme that catalyzes the first step of the tricarboxylic acid cycle (CITRIC ACID CYCLE). It catalyzes the reaction of oxaloacetate and acetyl CoA to form citrate and coenzyme A. This enzyme was formerly listed as EC 4.1.3.7.
The rate dynamics in chemical or physical systems.
An isomerase that catalyzes the conversion of chorismic acid to prephenic acid. EC 5.4.99.5.
A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that occurs in the natural environment (soil, water, and plant surfaces) or as an opportunistic human pathogen.
Infections with bacteria of the genus SERRATIA.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992).
A plant genus of the family BRASSICACEAE that contains ARABIDOPSIS PROTEINS and MADS DOMAIN PROTEINS. The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development.
The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990)
Proteins that originate from plants species belonging to the genus ARABIDOPSIS. The most intensely studied species of Arabidopsis, Arabidopsis thaliana, is commonly used in laboratory experiments.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants.
A family of anaerobic METHANOMICROBIALES whose cells are coccoid to straight or slightly curved rods. There are six genera.
An order of anaerobic, highly specialized methanogens, in the kingdom EURYARCHAEOTA. Its organisms are nonmotile or motile, with cells occurring as coccoid bodies, pseudosarcina, or rods. Families include METHANOMICROBIACEAE, Methanocorpusculaceae, and Methanospirillaceae.
The simplest saturated hydrocarbon. It is a colorless, flammable gas, slightly soluble in water. It is one of the chief constituents of natural gas and is formed in the decomposition of organic matter. (Grant & Hackh's Chemical Dictionary, 5th ed)
Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.
Deoxyribonucleic acid that makes up the genetic material of archaea.
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
The study of the origin, structure, development, growth, function, genetics, and reproduction of plants.
An essential aromatic amino acid that is a precursor of MELANIN; DOPAMINE; noradrenalin (NOREPINEPHRINE), and THYROXINE.
A plant division of GYMNOSPERMS consisting of cone-bearing trees and shrubs.
A product of hard secondary xylem composed of CELLULOSE, hemicellulose, and LIGNANS, that is under the bark of trees and shrubs. It is used in construction and as a source of CHARCOAL and many other products.
Concentrated pharmaceutical preparations of plants obtained by removing active constituents with a suitable solvent, which is evaporated away, and adjusting the residue to a prescribed standard.
PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING.
A condition characterized by a series of interrelated digital symptoms and joint changes of the lesser digits and METATARSOPHALANGEAL JOINTS of the FOOT. The syndrome can include some or all of the following conditions: hammer toe, claw toe, mallet toe, overlapping fifth toe, curly toe, EXOSTOSIS; HYPEROSTOSIS; interdigital heloma, or contracted toe.

Deletion plasmids from transformants of Pseudomonas aeruginosa trp cells with the RSF1010-trp hybrid plasmid and high levels of enzyme activity from the gene on the plasmid. (1/162)

A RSF1010-trp hybrid plasmid which contained the tryptophan operon of Escherichia coli was introduced into Pseudomonas aeruginosa trp cells by transformation. From the Trp+ transformants several deletion plasmids were obtained, and their physical maps with restriction endonucleases were constructed. P. aeruginosa trp cells with these plasmids showed at first more than 100 times higher levels of tryptophan synthetase beta activity over that of the control P. aeruginosa wild-type cells, but these levels were drastically decreased by 1 week of successive transfers of cultures. This decrease in enzyme activity was found to be due to the change on the plasmids but not to the host cells. The production of E. coli tryptophan synthetase beta enzyme in P. aeruginosa cells was proved by immunological test.  (+info)

The crystal structure of anthranilate synthase from Sulfolobus solfataricus: functional implications. (2/162)

Anthranilate synthase catalyzes the synthesis of anthranilate from chorismate and glutamine and is feedback-inhibited by tryptophan. The enzyme of the hyperthermophile Sulfolobus solfataricus has been crystallized in the absence of physiological ligands, and its three-dimensional structure has been determined at 2.5-A resolution with x-ray crystallography. It is a heterotetramer of anthranilate synthase (TrpE) and glutamine amidotransferase (TrpG) subunits, in which two TrpG:TrpE protomers associate mainly via the TrpG subunits. The small TrpG subunit (195 residues) has the known "triad" glutamine amidotransferase fold. The large TrpE subunit (421 residues) has a novel fold. It displays a cleft between two domains, the tips of which contact the TrpG subunit across its active site. Clusters of catalytically essential residues are located inside the cleft, spatially separated from clustered residues involved in feedback inhibition. The structure suggests a model in which chorismate binding triggers a relative movement of the two domain tips of the TrpE subunit, activating the TrpG subunit and creating a channel for passage of ammonia toward the active site of the TrpE subunit. Tryptophan presumably blocks this rearrangement, thus stabilizing the inactive states of both subunits. The structure of the TrpE subunit is a likely prototype for the related enzymes 4-amino 4-deoxychorismate synthase and isochorismate synthase.  (+info)

Inhibition of TATA-binding protein function by SAGA subunits Spt3 and Spt8 at Gcn4-activated promoters. (3/162)

SAGA is a 1.8-MDa yeast protein complex that is composed of several distinct classes of transcription-related factors, including the adaptor/acetyltransferase Gcn5, Spt proteins, and a subset of TBP-associated factors. Our results indicate that mutations that completely disrupt SAGA (deletions of SPT7 or SPT20) strongly reduce transcriptional activation at the HIS3 and TRP3 genes and that Gcn5 is required for normal HIS3 transcriptional start site selection. Surprisingly, mutations in Spt proteins involved in the SAGA-TBP interaction (Spt3 and Spt8) cause derepression of HIS3 and TRP3 transcription in the uninduced state. Consistent with this finding, wild-type SAGA inhibits TBP binding to the HIS3 promoter in vitro, while SAGA lacking Spt3 or Spt8 is not inhibitory. We detected two distinct forms of SAGA in cell extracts and, strikingly, one lacks Spt8. Conditions that induce HIS3 and TRP3 transcription result in an altered balance between these complexes strongly in favor of the form without Spt8. These results suggest that the composition of SAGA may be dynamic in vivo and may be regulated through dissociable inhibitory subunits.  (+info)

A Bacillus subtilis operon containing genes of unknown function senses tRNATrp charging and regulates expression of the genes of tryptophan biosynthesis. (4/162)

Strains of Bacillus subtilis containing a temperature-sensitive tryptophanyl-tRNA synthetase produce elevated levels of the tryptophan pathway enzymes, when grown at high temperatures in the presence of excess tryptophan. This increase is because of reduced availability of the tryptophan-activated trp RNA-binding attenuation protein (TRAP). To test the hypothesis that this elevated trp gene expression was caused by the overproduction of a transcript capable of binding and sequestering TRAP, a computer program was designed to search the B. subtilis genome sequence for additional potential TRAP binding sites. A region containing a stretch of (G/A)AG trinucleotide repeats, characteristic of a TRAP binding site, was identified in the yczA-ycbK operon. We show that transcriptional regulation of the yczA-ycbK operon is controlled by the T-box antitermination mechanism in response to the level of uncharged tRNA(Trp), and that the presence of a trpS1 mutant allele increases production of the yczA-ycbK transcript. Elevated yczA-ycbK expression was shown to activate transcription of the trp operon. Deletion of the yczA-ycbK operon abolishes the trpS1 effect on trp gene expression. The purpose of increasing expression of the genes of tryptophan biosynthesis in the trpS mutant would be to provide additional tryptophan to overcome the charged tRNA(Trp) deficiency. Therefore, in B. subtilis, as in Escherichia coli, transcription of the tryptophan biosynthetic genes is regulated in response to changes in the extent of charging of tRNA(Trp) as well as the availability of tryptophan.  (+info)

A Bacillus subtilis gene of previously unknown function, yhaG, is translationally regulated by tryptophan-activated TRAP and appears to be involved in tryptophan transport. (5/162)

Computer analysis of the Bacillus subtilis genome sequence revealed a gene with no previously attributed function, yhaG, specifying a transcript containing a presumptive binding site for the tryptophan-activated regulatory protein, TRAP. The presumptive TRAP binding site overlaps the yhaG Shine-Dalgarno sequence and translation initiation region. TRAP was shown to regulate expression of yhaG translationally. Production of the yhaG transcript in vivo was found to compete for the binding of TRAP to other known TRAP binding sites. YhaG is likely to be a transmembrane protein involved in tryptophan transport.  (+info)

The nucleotide sequence of the first externally suppressible--1 frameshift mutant, and of some nearby leaky frameshift mutants. (6/162)

Nine mutants within a 23 nucleotide sequence of the trpE gene of Salmonella typhimurium have been characterized. trpE91, a mutant which is externally suppressible has a single base deletion. Eight (or nine) nucleotides upstream of this deletion, two independently isolated mutations have the same transversion. In combination with trpE91 these mutations lead to partial restoration of synthesis of anthranilate synthetase in the absence of external suppressors. In the transversion the sequence A CA is changed to A AA and this new sequence may be the site where frameshifting occurs to allow leakiness. Leakiness is displayed by two further mutants of the same sign as trpE91, and one of the opposite sign, in the absence of any base substitution or external suppressors. Specific sequences, e.g., UUUC, may be especially prone to frameshifting and this sequence is created at the site of the +1 frameshift mutant which displays leakiness. In the new reading frame generated by the two -1 frame leaky mutants, a tryptophan codon is encountered. Leakiness is necessarily detected in the absence of tryptophan and under these conditions there will be a shortage of charged tryptophan tRNA. The possibility of such functional imbalance leading to frameshifting in these mutants is discussed.  (+info)

Increasing tryptophan synthesis in a forage legume Astragalus sinicus by expressing the tobacco feedback-insensitive anthranilate synthase (ASA2) gene. (7/162)

A cDNA clone that encodes a feedback-insensitive anthranilate synthase (AS), ASA2, isolated from a 5-methyl-tryptophan (Trp) (5MT)-resistant tobacco cell line under the control of the constitutive cauliflower mosaic virus 35S promoter, was introduced into the forage legume Astragalus sinicus by Agrobacterium rhizogenes with kanamycin selection. The 35S-ASA2 gene was expressed constitutively as demonstrated by northern-blot hybridization analyses and the presence of feedback-insensitive AS. Hairy root lines transformed with 35S-ASA2 grew in concentrations of up to 100 microM 5MT, whereas the controls were completely inhibited by 15 microM 5MT. Expression of the feedback-insensitive ASA2 resulted in a 1.3- to 5.5-fold increase in free Trp. Kinetic studies of the AS activity demonstrate the Trp feedback alterations and indicate that the ASA2 alpha-subunit can interact with the native A. sinicus beta-subunit to form an active enzyme. The ASA2 transcript and high free Trp were also detected in the leaves, stems, and roots of plants regenerated from the transformed hairy roots. Thus, we show for the first time that ASA2 can be used to transform plants of a different species to increase the levels of the essential amino acid Trp and impart 5MT resistance.  (+info)

Decay of mutualistic potential in aphid endosymbionts through silencing of biosynthetic loci: Buchnera of Diuraphis. (8/162)

Buchnera, the primary bacterial endosymbiont of aphids, is known to provision essential amino acids lacking in the hosts' diet of plant sap. The recent discovery of silenced copies of genes for tryptophan biosynthesis (trpEG) in certain Buchnera lineages suggests a decay in symbiotic functions in some aphid species. However, neither the distribution of pseudogenes among lineages nor the impact of this gene silencing on amino-acid availability in hosts has been assessed. In Buchnera of the aphid Diuraphis noxia, tandem repeats of these pseudogenes have persisted in diverse lineages, and thpEG pseudogenes have originated at least twice within this aphid genus. Measures of amino-acid concentrations in Diuraphis species have shown that the presence of the pseudogene is associated with a decreased availability of tryptophan, indicating that gene silencing decreases nutrient provisioning by symbionts. In Buchnera of Diuraphis, rates of nonsynonymous substitutions are elevated in functional trpE copies, supporting the hypothesis that pseudogene origin and persistence reflect a reduced selection for symbiont biosynthetic contributions. The parallel evolution of trpEG pseudogenes in Buchnera of Diuraphis and certain other aphid hosts suggests that either selection at the host level is not effective or that fitness in these aphids is not limited by tryptophan availability.  (+info)

ID HAMAR1_8_PE619 STANDARD; PRT; 489 AA. AC HAMAR1_8_PE619; Q5V448; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE RecName: Full=Anthranilate synthase component 1 1; EC=4.1.3 27;AltName: DE Full=Anthranilate synthase component I 1; (HAMAR1_8.PE619). GN Name=trpE1; OrderedLocusNames=rrnAC0709; OS HALOARCULA MARISMORTUI ATCC 43049. OC Archaea; Euryarchaeota; Halobacteria; Halobacteriales; Halobacteriaceae; OC Haloarcula. OX NCBI_TaxID=272569; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS HAMAR1_8.PE619. CC Haloarcula marismortui ATCC 43049 chromosome chromosome I, complete CC sequence. CC -!- ANNOTATIONS ORIGIN:TRPE1_HALMA CC -!- CATALYTIC ACTIVITY: Chorismate + L-glutamine = anthranilate + CC pyruvate + L-glutamate. CC -!- PATHWAY: Amino-acid biosynthesis; L-tryptophan biosynthesis; L- CC tryptophan from chorismate: step 1/5. CC -!- SUBUNIT: Tetramer of two components I and ...
Anthranilate synthase component I; Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine- binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentr [...] (508 aa ...
Anthranilate synthase component 1; Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine- binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentr [...] (497 aa ...
Anthranilate is an aromatic amine used industrially as an intermediate for the synthesis of dyes, perfumes, pharmaceuticals and other classes of products. Chemical synthesis of anthranilate is an unsustainable process since it implies the use of nonrenewable benzene and the generation of toxic by-products. In Escherichia coli anthranilate is synthesized from chorismate by anthranilate synthase (TrpED) and then converted to phosphoribosyl anthranilate by anthranilate phosphoribosyl transferase to continue the tryptophan biosynthetic pathway. With the purpose of generating a microbial strain for anthranilate production from glucose, E. coli W3110 trpD9923, a mutant in the trpD gene that displays low anthranilate producing capacity, was characterized and modified using metabolic engineering strategies. Sequencing of the trpED genes from E. coli W3110 trpD9923 revealed a nonsense mutation in the trpD gene, causing the loss of anthranilate phosphoribosyl transferase activity, but maintaining anthranilate
ID METEZ_1_PE1899 STANDARD; PRT; 522 AA. AC METEZ_1_PE1899; D7EBI9; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE SubName: Full=Anthranilate synthase component I; EC=4.1.3 27; DE (METEZ_1.PE1899). GN OrderedLocusNames=Metev_2002; OS METHANOHALOBIUM EVESTIGATUM Z-7303. OC Archaea; Euryarchaeota; Methanomicrobia; Methanosarcinales; OC Methanosarcinaceae; Methanohalobium. OX NCBI_TaxID=644295; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS METEZ_1.PE1899. CC Methanohalobium evestigatum Z-7303 chromosome, complete genome. CC sequence. CC -!- ANNOTATIONS ORIGIN:D7EBI9_METEZ CC -!- GENE_FAMILY: HOG000025142 [ FAMILY / ALN / TREE ] DR UniProtKB/Swiss-Prot; D7EBI9; -. DR EMBL; CP002069; ADI74831.1; -; Genomic_DNA. DR RefSeq; YP_003727627.1; NC_014253.1. DR ProteinModelPortal; D7EBI9; -. DR GeneID; 9347662; -. DR GenomeReviews; CP002069_GR; Metev_2002. DR KEGG; mev:Metev_2002; -. DR ...
Arabidopsis thaliana roots grow in a wavy pattern upon a slanted surface. A novel mutation in the anthranilate synthase alpha 1 (ASA1) gene, named trp5-2wvc1, and mutations in the tryptophan synthase alpha and beta 1 genes (trp3-1 and trp2-1, respectively) confer a compressed root wave phenotype on …
Bashiri G., Johnston JM., Evans GL., Bulloch EMM., Goldstone DC., Jirgis ENM., Kleinboelting S., Castell A., Ramsay RJ. and Manos-Turvey A. (2015) Structure and inhibition of subunit I of the anthranilate synthase complex of Mycobacterium tuberculosis and expression of the active complex. Acta Crystallographica Section D Biological Crystallography 71(11): 2297-2308. http://dx.doi.org/10.1107/S1399004715017216 ...
Regulation and Cell signalingRegulation and Cell signaling - no subcategorySex pheromones in Enterococcus faecalis and other Firmicutes Heptaprenyl diphosphate synthase component I (EC 2.5.1.30) ...
1I7S: The structures of anthranilate synthase of Serratia marcescens crystallized in the presence of (i) its substrates, chorismate and glutamine, and a product, glutamate, and (ii) its end-product inhibitor, L-tryptophan.
Alfa Chemistry is the worlds leading provider for special chemicals. We offer qualified products for 552-37-4(SODIUM ANTHRANILATE),please inquire us for 552-37-4(SODIUM ANTHRANILATE).
Mycobacteria tuberculosis (Mtb), the causative agent of tuberculosis, is responsible for more death in the world today than any other bacteria. As part of the Tuberculosis Structural Genomics Consortium (TBSGC), our research group previously determined the structure of anthranilate phosphoribosyl transferase (AnPRT) from Mtb. AnPRT is the second enzyme in the tryptophan biosynthetic pathway and was identified as a potential drug target through gene knockout experiments, which resulted in a strain of Mtb that was essentially avirulent even in immunodeficient mice. AnPRT catalyses a reaction between anthranilate and phosphoribosylpyrophosphate (PRPP), and the crystal structure of Mtb-AnPRT was originally determined with and without PRPP (PDB ID: 1ZVW and 2BPQ, respectively). In silico docking was used to predict the binding motif of anthranilate, the second substrate, surprisingly predicted two sites despite a 1:1 reaction ratio with PRPP. Previously, 165 compounds were screened for inhibitory ...
CIS-3-HEXENYL ANTHRANILATE 65405-76-7 NMR spectrum, CIS-3-HEXENYL ANTHRANILATE H-NMR spectral analysis, CIS-3-HEXENYL ANTHRANILATE C-NMR spectral analysis ect.
Anthranilate phosphoribosyltransferaseN-(5-phospho-D-ribosyl)-anthranilate + diphosphate = anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate ...
The Russian wheat aphid, Diuraphis noxia (Mordvilko), is a serious insect pest of wheat and barley. It affects the quality and yield of grain by sucking plant sap from the newest growth whilst toxic substances are injected ...
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F-, araC14, leuB6(Am), secA206(aziR), fhuA23, lacY1, proC83, tsx-67, purE42, glnX44(AS), galK2(Oc), λ-, trpE38, xthA15, his-208, rfbC1, mgl-51, argG77, rpsL109(strR), glpR201, xylA5, mtl-1, ilvA681, katG17::Tn10, thiE1, metA160 ...
1GTF: The structure of the trp RNA-binding attenuation protein (TRAP) bound to a 53-nucleotide RNA molecule containing GAGUU repeats
casSAR Dugability of P25170 | TRPC | Multifunctional tryptophan biosynthesis protein - Also known as TRPG_PHACH, TRPC. Trifunctional enzyme bearing the Gln amidotransferase (GATase) domain of anthranilate synthase, indole-glycerolphosphate synthase, and phosphoribosylanthranilate isomerase activities.
Shop Multifunctional tryptophan biosynthesis protein ELISA Kit, Recombinant Protein and Multifunctional tryptophan biosynthesis protein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
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The substrate saturation and temperature-dependent kinetic properties of soluble and membrane-bound forms of acetylcholinestarase (A C h E) from brain and butyrylcholinester- ase (B C h E) from heart and liver were examined. In simultaneous studies these parameters were also measured for A C h E in erythrocyte membranes and for B C h E in the serum from rat and humans. For both soluble and membrane-bound forms of the enzyme from the three tissues, two components were discernible. In the brain, K m of component I (high affinity) and component II (low affinity) was somewhat higher in membrane-bound form than that of the soluble form components, while the Vmax values were significantly higher by about five fold. In the heart, K m of component II was lower in membrane-bound form than in the soluble form, while Vmax for both the components was about four to six fold higher in the membrane-bound form. In the liver, Vmax was marginally higher for the two components of the membrane-bound enzyme; the K m ...
When cursor points to a box further details will be displayed in a toltip window. If you click on the box you will change to appropriate reaction scheme or enzyme specification.. ...
Page contains details about trp-RNA binding attenuation protein K35C mutant nanocages . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Phosphoribosylanthranilate isomerase and indoleglycerol-phosphate synthase: tryptophan biosynthetic enzymes from Thermotoga maritima ...
NAD+ synthase (EC 6.3.5.1) catalyzes the last step in the biosynthesis of nicotinamide adenine dinucleotide and is induced by stress factors such as heat shock and glucose limitation. The three-dimensional structure of NH3-dependent NAD+ synthetase from Bacillus subtilis, in its free form and in complex with ATP shows that the enzyme consists of a tight homodimer with alpha/beta subunit topology [(PUBMED:8895556)]. This domain is also found in guanosine 5-monophosphate (GMP) synthetase. GMP synthase catalyses the synthesis of GMP from XMP. The protein is a homodimer, but in some archaea it is a heterodimer composed of a glutamine amidotransferase subunit and a ATP pyrophosphatase subunit. In eucaryotes, bacteria, and some archaea the two catalytic units are encoded by a single gene, producing a two-domain-type GMP, with a GATase domain in the N-terminal half and a ATP-PPase domain in the C-terminal half. This entry represents the ATP pyrophosphatase domain. ...
Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).
The infestation of the cultivated grain crops by phloem feeding aphids has generated a great deal of interest over the years, due to the serious damage they cause to the crops and yield losses that result. The mechanism of the interaction between aphids and host plants remains largely unknown in spite of efforts to understand the basis of aphid feeding on grain crops. Greater efforts are required to explain the mechanism(s) of this interaction in order to achieve sustainable agriculture. This thesis focused on an investigation of the mechanism of feeding by the Russian wheat aphid, Diuraphis noxia Mordvilko (RWA) and the bird cherry-oat aphid, Rhopalosiphum padi L. (BCA) on barley and wheat cultivars. These two aphids co-occur naturally, but they inflict very different feeding effects on host plants. Structural and functional approaches were employed to investigate their feeding habits and these were then related to the observed differences in their host plants. Transmission electron microscopy ...
anthranilate synthase / indole-3-glycerol phosphate synthase / phosphoribosylanthranilate isomerase [EC:4.1.3.27 4.1.1.48 5.3.1.24 ...
PQS biosynthetic functions of several of the pqs products are suggested by their sequence homologies (Fig. 4). PhnA and PhnB presumably synthesize the anthranilate precursor of PQS from chorismate (4, 10). Gene pqsA encodes a product homologous to benzoate coenzyme A ligase, which may be involved in activating anthranilate for PQS synthesis. Genes pqsB, pqsC, and pqsD encode proteins homologous to β-keto-acyl-acyl carrier protein synthases and are presumably involved in the production of a long chain hydrocarbon which reacts with anthranilate in the PQS biosynthetic pathway (4). Gene pqsE is not homologous to any defined proteins and our results indicate that it is not required for PQS synthesis. Gene pqsH encodes a putative FAD-dependent monooxygenase that may be responsible for the addition of the hydroxyl group to PQS.. Although phnA and phnB were originally assumed to encode an anthranilate synthetase comprising part of the phenazine biosynthetic pathway (10), recent studies by Mavrodi et ...
Next-day shipping cDNA ORF clones derived from PET112 glutamyl-tRNA(Gln) amidotransferase subunit PET112 available at GenScript, starting from $99.00.
TY - JOUR. T1 - Short-term metabolic fate of 13N-labeled glutamate, alanine, and glutamine(amide) in rat liver. AU - Cooper, A. J.L.. AU - Nieves, E.. AU - Rosenspire, K. C.. AU - Filc-DeRicco, S.. AU - Gelbard, A. S.. AU - Brusilow, S. W.. PY - 1988/12/1. Y1 - 1988/12/1. N2 - Tracer quantities (in 0.2 ml) of 13N-labele glutamate, alanine, or glutamine(amide) were administered rapidly (≤ 2 s) via the portal vein of anesthetized adult male rats. Liver content of tracer at 5 s was 57 ± 6 (n = 6), 24 ± 1 (n = 3), and 69 ± 7 (n = 3) % of the injected dose, respectively. Portal-hepatic vein differences for the corresponding amino acids were 17 ± 6, 26 ± 8, and 19 ± 9% (n = 4), respectively, suggesting some export of glutamate and glutamine, but not of alanine, to the hepatic vein. Following L-[13N]glutamate administration, label rapidly appeared in liver alanine and aspartate (within seconds). The data emphasize the rapidity of nitrogen exchange via linked transaminases. By 30 s following ...
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Decreases in enzyme activity often have little effect on the flux carried by the pathway. Similarly, up-modulation of single genes, and hence of the dependent enzyme concentrations, is frequently found to be ineffective in increasing the flux in the pathway in which the enzyme occurs. This insensitivity to enzyme variation is demonstrated experimentally for five separate enzymes in the tryptophan synthesis system of yeast, first by down-modulation of the gene dose and secondly by increasing the dose using multi-copy vectors. Such a lack of response is discussed in terms of the concepts of metabolic control analysis. When these five enzymes, however, were simultaneously increased by a multi-copy vector carrying all five genes, a substantial elevation of the flux to tryptophan was observed. These findings revealed a new phenomenon, namely the more than additive effects on the flux of simultaneous elevations of several enzyme activities. ...
3-(2-Aminoethyl)indol-5-ol p-aminobenzoate dihydrochloride | C17H19Cl2N3O2 | CID 29683 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.
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The pab1 gene of the basidiomycete Coprinus cinereus encodes PABA synthase, necessary for para-aminobenzoic acid production. The C. cinereus protein is bifunctional with an N-terminal glutamine amidotransferase domain and a C-terminal chorismate amination domain. In most bacteria, these two functions are encoded in separate genes (e.g., pabA and pabB of E. coli). Fused PABA synthases have so far been detected in actinomycetes, Plasmodium falciparum, fungi and Arabidopsis thaliana. Phylogenetic analysis shows that the fused PAB sequences form a tight group that also includes uncharacterized PabB homologues from several bacteria. Unfused bacterial PabA proteins group with the glutamine amidotransferase subunits of bacterial anthranilate synthases, independent of organismal systematics, indicating a complex and perhaps independent evolutionary origin. In contrast, unfused PabB group and fused PabA/B proteins form a monophyletic group on a branch separate from the chorismate amination subunits of
Forty single gene mutations in Chlamydomonas reinhardtii were isolated based on resistance to the compound 5-methyl anthranilic acid (5-MAA). In other organisms, 5-MAA is converted to 5-methyltryptophan (5-MT) and 5-MT is a potent inhibitor of anthranilate synthase, which catalyzes the first committed step in tryptophan biosynthesis. The mutant strains fall into two phenotypic classes based on the rate of cell division in the absence of 5-MAA. Strains with class I mutations divide more slowly than wild-type cells. These 17 mutations map to seven loci, which are designated MAA1 to MAA7. Strains with class II mutations have generation times indistinguishable from wild-type cells, and 7 of these 23 mutations map to loci defined by class I mutations. The remainder of the class II mutations map to 9 other loci, which are designated MAA8-MAA16. The maa5-1 mutant strain excretes high levels of anthranilate and phenylalanine into the medium. In this strain, four enzymatic activities in the tryptophan ...
TY - JOUR. T1 - TrpM, a Small Protein Modulating Tryptophan Biosynthesis and Morpho-Physiological Differentiation in Streptomyces coelicolor A3(2). AU - Puglia, Anna Maria. AU - Botta, Luigi. AU - Giardina, Anna. AU - Gallo, Giuseppe. AU - Sutera, Alberto. AU - Palazzotto, Emilia. AU - Scaloni, Andrea. AU - Renzone, Giovanni. AU - Palazzotto, Emilia. PY - 2016. Y1 - 2016. N2 - In the model actinomycete Streptomyces coelicolor A3(2), small open reading frames encoding proteins with unknown functions were identified in several amino acid biosynthetic gene operons, such as SCO2038 (trpX) in the tryptophan trpCXBA locus. In this study, the role of the corresponding protein in tryptophan biosynthesis was investigated by combining phenotypic and molecular analyses. The 2038KO mutant strain was characterized by delayed growth, smaller aerial hyphae and reduced production of spores and actinorhodin antibiotic, with respect to the WT strain. The capability of this mutant to grow on minimal medium was ...
Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).
Mycobacterium tuberculosis is an important pathogen of mammals that relies on 2-hydroxyphenyloxazoline-containing siderophore molecules called mycobactins for the acquisition of iron in the restrictive environment of the mammalian macrophage. These compounds have been proposed to be biosynthesized through the action of a cluster of genes that include both nonribosomal peptide synthase and polyketide synthase components. One of these genes encodes a protein, MbtB, that putatively couples activated salicylic acid with serine or threonine and then cyclizes this precursor to the phenyloxazoline ring system. We have used gene replacement through homologous recombination to delete the mbtB gene and replace this with a hygromycin-resistance cassette in the virulent strain of M. tuberculosis H37Rv. The resulting mutant is restricted for growth in iron-limited media but grows normally in iron-replete media. Analysis of siderophore production by this organism revealed that the biosynthesis of all salicylate
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Sodium Dodecyl Sulfate, or SDS, is an anionic detergent with widespread use in industrial and household cleaning products, scientific laboratories, and personal care products such as toothpaste and shampoo. The potential toxicity of SDS has been well-characterized in whole organism studies and its potential effects on the environment continue to be studied. Herein, we undertake a chemical-genetic screen to explore whether low concentrations of SDS have any discernible effects at the cellular level. Our screen of the homozygous diploid yeast deletion collection identified numerous gene deletions that confer sensitivity to SDS. Subsequent bioinformatic and biological analyses reveal that yeast unable to synthesize tryptophan are especially sensitive to the presence of SDS. Interestingly, even wild-type yeast with an intact tryptophan biosynthetic pathway exhibit growth defects in the presence of SDS on media lacking tryptophan. Altogether, we have shown that low levels of SDS, primarily through ...
FIGURE 1. Culture of splenic T cells or purified CD8+ T cells with IDO+ DCs, or with LT and kynurenines, results in selective CD3ζ down-regulation that is concomitant with tryptophan consumption and kynurenine production and is mediated by GCN2. A, Total T cells and purified CD4+ or CD8+ fractions from DBA/2 mice were cultured for 48 h with soluble anti-CD3 (1 μg/ml) and IDO− DCs (thick line histogram) or IDO+ DCs with (thin line histogram) or without (gray-filled histogram) 1-MT. Cells were labeled for surface CD3, CD4, or CD8 with the respective FITC-labeled reagents, and were then fixed, permeabilized and stained for total ζ-chain expression by means of PE-labeled anti-mouse CD3ζ. Filled histogram indicates isotype controls. Data are from one experiment representative of four. B, Concentrations of tryptophan (TRY) and different kynurenines (mean ± SD of three independent experiments) were measured in 24-h supernatants of total T cells and IDO+ DCs. The initial tryptophan concentration ...
cd03141 (PSSM ID: 153235): Conserved Protein Domain Family GATase1_Hsp31_like, Type 1 glutamine amidotransferase (GATase1)-like domain found in proteins similar to Escherichia coli Hsp31 protein (EcHsp31)
The TriRNA Pure Kit is a spin column system for purification of high-quality total RNA from a variety of samples. Initially, samples are homogenized in TRIzol® Reagent, GENEzol™ Reagent or other phenol, guanidine isothiocyanate reagents. Chloroform phase separation or isopropanol RNA precipitation is not required.
Anthranilate phosphoribosyltransferase from the hyperthermophilic archaeon Sulfolobus solfataricus (ssAnPRT) is encoded by the sstrpD gene and catalyzes the reaction of anthranilate (AA) with a complex of Mg(2+) and 5-phosphoribosyl-alpha1-pyrophosphate (Mg.PRPP) to N-(5-phosphoribosyl)-anthranilate (PRA) and pyrophosphate (PP(i)) within tryptophan biosynthesis. The ssAnPRT enzyme is highly thermostable (half-life at 85 degrees C = 35 min) but only marginally active at ambient temperatures (turnover number at 37 degrees C = 0.33 s(-1)). To understand the reason for the poor catalytic proficiency of ssAnPRT, we have isolated from an sstrpD library the activated ssAnPRT-D83G + F149S double mutant by metabolic complementation of an auxotrophic Escherichia coli strain. Whereas the activity of purified wild-type ssAnPRT is strongly reduced in the presence of high concentrations of Mg(2+) ions, this inhibition is no longer observed in the double mutant and the ssAnPRT-D83G single mutant. The ...
The trp operon is an operon-a group of genes that is used, or transcribed, together-that codes for the components for production of tryptophan. The trp operon is present in many bacteria, but was first characterized in Escherichia coli. The operon is regulated so that when tryptophan is present in the environment, the genes for tryptophan synthesis are not expressed. It was an important experimental system for learning about gene regulation, and is commonly used to teach gene regulation. Discovered in 1953 by Jacques Monod and colleagues, the trp operon in E. coli was the first repressible operon to be discovered. While the lac operon can be activated by a chemical (allolactose), the tryptophan (Trp) operon is inhibited by a chemical (tryptophan). This operon contains five structural genes: trp E, trp D, trp C, trp B, and trp A, which encode tryptophan synthetase. It also contains a repressive regulator gene called trp R. trp R has a promoter where RNA polymerase binds and synthesizes mRNA for a ...
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富永真琴「温度感受性TRP チャネルの生理機能と創薬標的としての意義」,第38 回生体膜と薬物の相互作用 シンポジウム,名古屋,2016 年11 月. ...
Their roles are: TrpE (P00895): Anthranilate synthase produces anthranilate. TrpD (P00904): Cooperates with TrpE. TrpC (P00909 ... The Indole-3-glycerol-phosphate synthase on the same protein then turns the product into (1S,2R)-1-C-(indol-3-yl)glycerol 3- ... Phosphoribosylanthranilate isomerase domain first turns N-(5-phospho-β-D-ribosyl)anthranilate into 1-(2-carboxyphenylamino)-1- ...
Tryptophan biosynthesis involves conversion of chorismate to anthranilate using anthranilate synthase. This enzyme requires ... Anthranilate synthase is regulated by the gene products of trpE and trpG. trpE encodes the first subunit, which binds to ... Anthranilate synthase is also regulated by feedback inhibition: tryptophan is a co-repressor to the TrpR repressor. The ... Enzymes involved in this biosynthesis include acetolactate synthase (also known as acetohydroxy acid synthase), acetohydroxy ...
"The Arabidopsis thaliana trp5 mutant has a feedback-resistant anthranilate synthase and elevated soluble tryptophan". Plant ... "Isolation of cDNAs encoding the tryptophan pathway enzyme indole-3-glycerol phosphate synthase from Arabidopsis thaliana". ...
... made from shikimate and is then attacked by nascent ammonia to produce the ortho aminobenzoate isomer by anthranilate synthase ... Enaminomycin C is formed from anthranilate, which is made via the shikimate pathway as well. Subsequent oxidation is followed ... Farinamycin is made of three different components: 3-OH-anthranilate (3-HAA), 3,4-aminohydroxybenzamide (3,4-AHBAm) and the ... natural product Enaminomycin C. 3-OH anthranilate is derived from the central shikimate pathway metabolite chorismate. ...
... may refer to: Time-resolved photon emission Anthranilate synthase, an enzyme This disambiguation page lists articles ...
... a virtual library of potential herbicidal inhibitors of the enzyme anthranilate synthase was generated by keeping the core ...
... anthranilate N-benzoyltransferase, biphenyl synthase, glycine N-benzoyltransferase, ornithine N-benzoyltransferase and ... It is a substrate in the formation of xanthonoids in Hypericum androsaemum by benzophenone synthase, condensing a molecule of ...
The Structures of Anthranilate Synthase of Serratia Marcescens Crystallized in the Presence of (i) its substrates, Chorismate ...
... anthranilate synthase EC 4.1.3.28: now EC 2.3.3.3 EC 4.1.3.29: now EC 2.3.3.4 EC 4.1.3.30: methylisocitrate lyase EC 4.1.3.31: ... 11-diene synthase EC 4.2.3.25: S-linalool synthase EC 4.2.3.26: R-linalool synthase EC 4.2.3.27: isoprene synthase EC 4.2.3.28 ... d-cadinene synthase EC 4.2.3.14: pinene synthase EC 4.2.3.15: myrcene synthase EC 4.2.3.16: (4S)-limonene synthase EC 4.2.3.17 ... chorismate synthase EC 4.2.3.6: trichodiene synthase EC 4.2.3.7: pentalenene synthase EC 4.2.3.8: casbene synthase EC 4.2.3.9: ...
... anthranilate phosphoribosyltransferase MeSH D08.811.600.085 - anthranilate synthase MeSH D08.811.600.116 - aspartate ... riboflavin synthase MeSH D08.811.913.225.825 - spermidine synthase MeSH D08.811.913.225.912 - spermine synthase MeSH D08.811. ... anthranilate synthase MeSH D08.811.520.224.600.700 - isocitrate lyase MeSH D08.811.520.224.800 - tryptophanase MeSH D08.811. ... nitric oxide synthase type i MeSH D08.811.682.664.500.772.500 - nitric oxide synthase type ii MeSH D08.811.682.664.500.772.750 ...
... mycocerosate synthase EC 2.3.1.112: D-tryptophan N-malonyltransferase EC 2.3.1.113: anthranilate N-malonyltransferase EC 2.3. ... 2-ethylmalate synthase EC 2.3.3.7: 3-ethylmalate synthase EC 2.3.3.8: ATP citrate synthase EC 2.3.3.9: malate synthase EC 2.3. ... synthase EC 2.3.3.2: decylcitrate synthase EC 2.3.3.3: citrate (Re)-synthase EC 2.3.3.4: decylhomocitrate synthase EC 2.3.3.5: ... synthase EC 2.4.1.12: cellulose synthase (UDP-forming) EC 2.4.1.13: sucrose synthase EC 2.4.1.14: sucrose-phosphate synthase EC ...
It is an intermediate in the biosynthesis of tryptophan, where it stays inside the tryptophan synthase molecule between the ... Indole is biosynthesized in the shikimate pathway via anthranilate. ...
... anthranilate-CoA ligase EC 6.2.1.33: 4-chlorobenzoate-CoA ligase EC 6.2.1.34: trans-feruloyl-CoA synthase EC 6.2.1.35: ACP-SH: ... arginine b-lactam-synthase EC 6.3.4.1: GMP synthase. Now included in EC 6.3.5.2, GMP synthase (glutamine-hydrolysing). EC 6.3. ... imidazole ribonucleotide synthase EC 6.3.4.19: tRNAIle-lysidine synthase EC 6.3.4.20: 7-cyano-7-deazaguanine synthase EC 6.3. ... glutathionylspermidine synthase EC 6.3.1.9: trypanothione synthase EC 6.3.1.10: adenosylcobinamide-phosphate synthase EC 6.3. ...
... pentalenolactone F synthase EC 1.14.12.1: anthranilate 1,2-dioxygenase (deaminating, decarboxylating) EC 1.14.12.2: now EC 1.14 ... clavaminate synthase EC 1.14.11.22: flavone synthase EC 1.14.11.23: flavonol synthase EC 1.14.11.24: 2'-deoxymugineic-acid 2'- ... berbamunine synthase EC 1.14.21.4: salutaridine synthase EC 1.14.21.5: (S)-canadine synthase EC 1.14.21.6: lathosterol oxidase ... aureusidin synthase EC 1.21.3.7: tetrahydrocannabinolic acid synthase EC 1.21.3.8: cannabidiolic acid synthase EC 1.21.4.1: D- ...
Anthranilate-CoA ligase EC 6.2.1.33: 4-chlorobenzoate-CoA ligase EC 6.2.1.34: Trans-feruloyl-CoA synthase EC 6.2.1.35: ACP-SH: ... Thiazole synthase EC 2.8.1.11: Molybdopterin synthase sulfurtransferase EC 2.8.1.12: Molybdopterin synthase EC 2.8.1.13: tRNA- ... Biotin synthase EC 2.8.1.7: Cysteine desulfurase EC 2.8.1.8: Lipoyl synthase EC 2.8.1.9: Molybdenum cofactor sulfurtransferase ... EC 1.21.3 Isopenicillin N synthase EC 1.21.3.1 Tetrahydrocannabinolic acid synthase EC 1.21.3.7 Category:EC 1.21.4 Category:EC ...
2. N-formyl-L-kynurenine is converted to anthranilate by the pathway-specific kynureninase, AntP. 3. Anthranilate is activated ... Antimycins are produced by a non-ribosomal peptide synthetase (NRPS)/polyketide synthase (PKS) assembly complex which acts as ... 4. Anthranilate is converted to 3-aminosalicylate by a multicomponent oxygenase, AntHIJKL. 5. 3-Aminosalicylate is presented to ...
In another study involving the S. solfataricus indole-3-glycerol phosphate synthase TIM barrel protein, a conserved βαβαβ ... anthranilate (PRA) to 1-(O-carboxyphenylamino)- 1'-deoxyribulose-5'-phosphate (CdRP). PriA is a TIM barrel enzyme that ... These clamps (or hydrophobic side chain bridge analogs) are conserved in 3 indole-3-glycerolphosphate synthase TIM barrel ... indole-3-glycerol phosphate synthase, by hydrogen exchange mass spectrometry and Gō model simulation". Journal of Molecular ...
EC 2.9.1.2: O-phospho-L-seryl-tRNA(Sec):L-selenocysteinyl-tRNA synthase ... EC 6.2.1.32: Anthranilate--CoA ligase. *EC 6.2.1.33: 4-chlorobenzoate--CoA ligase ... 2-Succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase EC 2.2.1.9 ... EC 6.2.1.38: (2,2,3-trimethyl-5-oxocyclopent-3-enyl)acetyl-CoA synthase ...
anthranilate metabolic process. • NAD biosynthetic process. • 'de novo' NAD biosynthetic process from tryptophan. ... regeneration of methionine: Methionine synthase/Homocysteine methyltransferase. *Betaine-homocysteine methyltransferase. * ...
... whereas its 3 products are anthranilate, pyruvate, and L-glutamate. In enzymology, an anthranilate synthase (EC 4.1.3.27) is an ... In enzymology, an anthranilate synthase (EC 4.1.3.27) is an enzyme that catalyzes the chemical reaction chorismate + L- ... Poulsen, C; Bongaerts, RJ; Verpoorte, R (March 1993). "urification and characterization of anthranilate synthase from ... The systematic name of this enzyme class is chorismate pyruvate-lyase (amino-accepting; anthranilate-forming). Other names in ...
Anthranilate synthase component I-like (IPR019999)*Anthranilate synthase component I, TrpE-like, bacterial (IPR005257) ...
In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine ... to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high ... Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the ... Anthranilate synthase component 1 (trpE), Anthranilate synthase component 1 (C7M65_09250), Anthranilate synthase component 1 ( ...
In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine ... to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high ... Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the ... Anthranilate synthase component 1 (trpE), Anthranilate synthase component 1 (trpE), Bifunctional protein TrpGD (trpGD) ...
Overexpresion of the feedback-insensitive anthranilate synthase gene in tobacco causes tryptophan accumulation * * TSAI F-Y ...
Anthranilate is a common precursor of all these compounds and anthranilate synthase (AS) catalyzes the synthesis of ... 1992) Two anthranilate synthase genes in Arabidopsis: defense-related regulation of the tryptophan pathway. Plant Cell 4:721- ... Anthranilate synthase (AS) is a key enzyme in the synthesis of tryptophan (Trp), indole-3-acetic acid, and indole alkaloids. ... 1996) Anthranilate synthase from Ailanthus altissima cell suspension cultures. Phytochemistry 41:395-402. ...
Anthranilate synthase (AS) is a rate-limiting enzyme in the TIA pathway. The transgenic C. roseus hairy root line ... Anthranilate synthase (AS) catalyzes chorismate to anthranilate, which is considered to be the rate-limiting step in indole ... Anthranilate synthase (AS) is a rate-limiting enzyme in the TIA pathway. The transgenic C. roseus hairy root line ... 2 Anthranilate synthase genes in Arabidopsis - defense-related regulation of the tryptophan pathway. Plant Cell. 1992;4(6):721- ...
... venezuelae has one or more additional genes for anthranilate synthase. In alignments with anthranilate synthase genes from ... The anthranilate synthase activity in S. venezuelae cell extracts was inhibited by tryptophan, although only at high ... encodes a 624 amino acid sequence similar to the overall sequence of the two subunits of anthranilate synthase. The two-thirds ... increasing the concentration of exogenous tryptophan decreased the formation of anthranilate synthase; similar evidence of ...
Their roles are: TrpE (P00895): Anthranilate synthase produces anthranilate. TrpD (P00904): Cooperates with TrpE. TrpC (P00909 ... The Indole-3-glycerol-phosphate synthase on the same protein then turns the product into (1S,2R)-1-C-(indol-3-yl)glycerol 3- ... Phosphoribosylanthranilate isomerase domain first turns N-(5-phospho-β-D-ribosyl)anthranilate into 1-(2-carboxyphenylamino)-1- ...
... Other foods you could consider avoiding are: sugar, dairy products, deep-fried ... Discover out what actual customers must say about acne free in 3 days anthranilate synthase feedback inhibition does it work ... Youll be able to nonetheless exfoliate the pores and skin, which is able to anthranilate synthase feedback inhibition take ... Luckily, there are prolific acne treatment for this condition that anthranilate synthase feedback inhibition diminish each the ...
Graf, R., Mehmann, B., Braus, G. H. (1993). "Analysis of feedback-resistant anthranilate synthases from Saccharomyces ... "Nucleotide sequence of Saccharomyces cerevisiae genes TRP2 and TRP3 encoding bifunctional anthranilate synthase: indole-3- ...
The structures of anthranilate synthase of Serratia marcescens crystallized in the presence of (i) its substrates, chorismate ... ANTHRANILATE SYNTHASE A, C 519 Serratia marcescens EC#: 4.1.3.27 IUBMB Mutation: P60R, V83L, L130I, V164L, H459V, H461N, R492P ... ANTHRANILATE SYNTHASE FROM SERRATIA MARCESCENS IN COMPLEX WITH ITS END PRODUCT INHIBITOR L-TRYPTOPHAN. *DOI: 10.2210/pdb1i7s/ ...
... and expression of ANTHRANILATE SYNTHASE -alpha1 (ASA1), a gene involved in IAA-biosynthesis. ASA1 and ANTHRANILATE SYNTHASE - ... Adventitious roots; Anthranilate synthase genes; In vitro culture; Indole-3-acetic acid; Indole-3-acetic acid efflux carriers; ... in Arabidopsis thaliana thin cell layers by conversion into indole-3-acetic acid and stimulation of anthranilate synthase ... in Arabidopsis thaliana thin cell layers by conversion into indole-3-acetic acid and stimulation of anthranilate synthase ...
Anthranilate synthase component II. CGGGCTGCAT[A/G]CCAGAGCTGC. 1. 724118. G. G. A. 2009V-1096. ...
... and mutations in the tryptophan synthase alpha and beta 1 genes (trp3-1 and trp2-1, respectively) confer a compressed root wave ... A novel mutation in the anthranilate synthase alpha 1 (ASA1) gene, named trp5-2wvc1, ... A novel mutation in the anthranilate synthase alpha 1 (ASA1) gene, named trp5-2wvc1, and mutations in the tryptophan synthase ... When trp5-2wvc1 seedlings are grown on media supplemented with anthranilate metabolites, their roots wave like wild type. ...
DAHP synthase; tyrA, prephenate dehydrogenase; quiC, 3-dehydroshikimate dehydratase; trpE, anthranilate synthase; hpd, 4- ... In P. putida S12 TPL3, genome analysis revealed a mutation in the gene encoding the anthranilate synthase (trpE), resulting in ... 2015). Metabolic engineering of Pseudomonas putida KT2440 to produce anthranilate from glucose. Front. Microbiol. 6:1310. doi: ... Different Pseudomonas strains have been used for the production of a range of bio-based aromatics including anthranilate, ...
DR SUPFAM; SSF56322; TRPE_1_chor_bd; 1. DR HOGENOMDNA; METMJ_1.PE71; -. KW anthranilate synthase; KW Complete proteome; Lyase. ... 3, Last annotation update) DE SubName: Full=Anthranilate synthase, component I; EC=4.1.3 27; DE (METMJ_1.PE71). GN ... anthranilate synthase activity; IEA:EC. DR GO; GO:0009058; P:biosynthetic process; IEA:InterPro. DR InterPro; IPR005801; ADC_ ... synthase. DR InterPro; IPR019999; Anth_synth_I. DR InterPro; IPR006805; Anth_synth_I_N. DR InterPro; IPR015890; Chorismate-bd_C ...
PEP, phosphoenolpyruvate; E4P, erythrose 4-phosphate; CM, chorismate mutase; AS, anthranilate synthase. ... Chorismate may be used by the enzyme anthranilate synthase in the synthesis of Trp or converted to prephenate, which is the ... 1996). Tissue localization of u.v.-B-screening pigments and of chalcone synthase mRNA in needles of Scots pine seedlings. New ... NADH-dependent glutamate synthase; L-Glu, L-glutamate; L-Gln, L-glutamine; 2-OG, 2-oxoglutarate. ...
DR KEGG; mif:Metin_0192; -. DR OMA; EDSTIMA; -. DR GO; GO:0004049; F:anthranilate synthase activity; IEA:InterPro. DR GO; GO: ... 3, Last annotation update) DE SubName: Full=Glutamine amidotransferase of anthranilate synthase; DE (METIM_1.PE190). GN ...
Interchangeability of the two anthranilate synthases and evolutionary implications. J Bacteriol 172:884-900. ... 1990) Identification and characterization of genes for a second anthranilate synthetase in Pseudomonas aeruginosa: ...
Thermodynamics of reactions catalyzed by anthranilate synthase. Biophysical Chememistry: 84 (2000): 45-64. Byrnes, W. M.,* ... Extrinsic factors potassium chloride and glycerol induce thermostability in recombinant anthranilate synthase from ... Extrinsic factors potassium chloride and glycerol induce thermostability in recombinant anthranilate synthase from ...
Tryptophan biosynthesis involves conversion of chorismate to anthranilate using anthranilate synthase. This enzyme requires ... Anthranilate synthase is regulated by the gene products of trpE and trpG. trpE encodes the first subunit, which binds to ... Anthranilate synthase is also regulated by feedback inhibition: tryptophan is a co-repressor to the TrpR repressor. ... Enzymes involved in this biosynthesis include acetolactate synthase (also known as acetohydroxy acid synthase), acetohydroxy ...
Two anthranilate synthase genes in Arabidopsis : defenserelated regulation of the tryptophan pathway NIYOGI K. K. ... Use of a feedback-insensitive α subunit of anthranilate synthase as a selectable marker for transformation of rice and potato ... Suppressors of trp1 fluorescence identify a new Arabidopsis gene, TRP4, encoding the anthranilate synthase β subunit NIYOGI KK ... Expression of a feedback insensitive anthranilate synthase gene from tobacco increases free tryptophan in soybean plants INABA ...
Anthranilate synthase (AhANS). 2. Production of tryptophan pathway metabolites as part of an Arabidopsis defense response (e.g ... The synthesis of saponins and expression of respective genes (squalene synthase, squalene epoxidase, and β-amyrin synthase) is ... chalcone synthase; 4CL, 4-coumarate:CoA ligase; DMID, 7,2′-dihydroxy-4′-methoxy-isoflavonol dehydratase; FSII, flavone synthase ... Squalene synthase (AhSqS). 1. The downregulation of SqS reduced stomatal conductance and conserved more moisture; it also led ...
anthranilate synthase / indole-3-glycerol phosphate synthase / phosphoribosylanthranilate isomerase [EC:4.1.3.27 4.1.1.48 5.3. ... 4.1.1.48 indole-3-glycerol-phosphate synthase. CNBH0530. 4.1.3 Oxo-acid-lyases. 4.1.3.27 anthranilate synthase. CNBH0530. 5. ...
Interchangeability of the two anthranilate synthases and evolutionary implications. J. Bacteriol. 1990, 172, 884-900. [Google ... Identification and characterization of genes for a second anthranilate synthase in Pseudomonas aeruginosa: ... C. violaceum CV026 lacks the autoinducer synthase CviI and thus requires exogenous C6-HSL for violacein formation, which is QS- ...
1981 Subunit structure of anthranilate synthase from Neurospora crassa: preparation and characterization of a protease-free ... Strains carrying the trp-2 mutation were supplemented with 10 μg/ml anthranilate. Anthranilate is a metabolic precursor of ... The trp-2 gene encodes anthranilate synthetase (Keeseyet al. 1981). The mtrSR33 trp-241 mat A strain was crossed to lab strain ... 10 μg/ml anthranilate, 2% agar). For RNA analyses, tissue was grown and synchronized by light-to-dark transfers following ...
Anthranilate Synthase* * Diabetic Nephropathies / etiology* * Fructosephosphates / metabolism * Hexosamines / biosynthesis* * ...
Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction. ... Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction. ... Marker: nopaline synthase as marker gene with additional border sequence between the Bt gene and the nos gene. ... 31, pHD1050 contains the chimeric Bt2 gene under the control of the Pnos promotor, as well as the nopaline synthase gene ...
Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction. ...
  • The result implies that S. venezuelae has one or more additional genes for anthranilate synthase. (microbiologyresearch.org)
  • In alignments with anthranilate synthase genes from other organisms, ORF2 from S. venezuelae most closely resembled genes for phenazine biosynthesis in Pseudomonas. (microbiologyresearch.org)
  • Nucleotide sequence of Saccharomyces cerevisiae genes TRP2 and TRP3 encoding bifunctional anthranilate synthase: indole-3-glycerol phosphate synthase. (ymdb.ca)
  • IBA induced IAA-efflux (PIN1) and IAA-influx (AUX1/LAX3) genes, IAA-influx carriers activities, and expression of ANTHRANILATE SYNTHASE -alpha1 (ASA1), a gene involved in IAA-biosynthesis. (nih.gov)
  • A novel mutation in the anthranilate synthase alpha 1 (ASA1) gene, named trp5-2wvc1, and mutations in the tryptophan synthase alpha and beta 1 genes (trp3-1 and trp2-1, respectively) confer a compressed root wave phenotype on tilted agar surfaces. (nih.gov)
  • A key enzyme for SA biosynthesis is isochorismate synthase (ICS), which converts chorismate into isochorismate, and for which there are two genes in Arabidopsis thaliana . (portlandpress.com)
  • The sequences of genes encoding anthranilate synthase alpha 1 (ASA1), tryptophan synthase alpha (TSA), indole-3-glycerol phosphate lyase BX1 (Bx1) and cytochrome P450 monooxygenase BX2 (Bx2) were obtained. (usda.gov)
  • The cbb3 operon contains genes predicted to encode anthranilate synthase components I and II, catalyzing the formation of anthranilate and pyruvate from chorismate. (biomedcentral.com)
  • A number of genes essential for Trp production have been isolated in A. brasilense, including trpE(G) which codes for anthranilate synthase, the key enzyme in Trp biosynthesis. (agriville.com)
  • Sequencing of the trpED genes from E. coli W3110 trpD9923 revealed a nonsense mutation in the trpD gene, causing the loss of anthranilate phosphoribosyl transferase activity, but maintaining anthranilate synthase activity, thus causing anthranilate accumulation. (biomedcentral.com)
  • The effects of expressing genes encoding a feedback inhibition resistant version of the enzyme 3-deoxy-D- arabino -heptulosonate-7-phosphate synthase ( aroG fbr ), transketolase ( tktA ), glucokinase ( glk ) and galactose permease ( galP ), as well as phosphoenolpyruvate:sugar phosphotransferase system (PTS) inactivation on anthranilate production capacity, were evaluated. (biomedcentral.com)
  • In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. (uniprot.org)
  • Characterization of Rice Anthranilate Synthase α-Subunit GenesOASA1 and OASA2. (plantphysiol.org)
  • ASA1 and ANTHRANILATE SYNTHASE -beta1 (ASB1), the other subunit of the same enzyme, positively affected AR-formation in the presence of exogenous IBA, because the AR-response in the TCLs of their mutant wei2wei7 was highly reduced. (nih.gov)
  • gene encoding a feedback-insensitive α-subunit of anthranilate synthase (AS, EC 4.1.3.27). (nii.ac.jp)
  • ASA1 encodes the alpha subunit of anthranilate synthase, which catalyzes the rate-limiting step of tryptophan synthesis. (cpib.ac.uk)
  • Ihara K, Watanabe S, Sugimura K, Katagiri I, Mukohata Y. Identification of proteolipid from an extremely halophilic archaeon Halobacterium salinarum as an N,N'-dicyclohexyl-carbodiimide binding subunit of ATP synthase. (labome.org)
  • In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia (Probable). (uniprot.org)
  • Their roles are: TrpE (P00895): Anthranilate synthase produces anthranilate. (wikipedia.org)
  • In Escherichia coli , the first two reactions in the L-Trp biosynthetic pathway are catalyzed by the enzyme complex anthranilate synthase-phosphoribosyl transferase (TrpE-TrpD). (biomedcentral.com)
  • Component I (TrpE) catalyses the conversion of chorismate and glutamine to anthranilate, glutamate and pyruvate. (biomedcentral.com)
  • The anthranilate synthase activity is the result of aminase and amidotransferase activities that are encoded by trpE and the amino terminal region encoded by trpGD , respectively (Fig. 2a ). (biomedcentral.com)
  • The Indole-3-glycerol-phosphate synthase on the same protein then turns the product into (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate. (wikipedia.org)
  • In enzymology, an anthranilate synthase (EC 4.1.3.27) is an enzyme that catalyzes the chemical reaction chorismate + L-glutamine ⇌ {\displaystyle \rightleftharpoons } anthranilate + pyruvate + L-glutamate 2 Thus, the two substrates of this enzyme are chorismate and L-glutamine, whereas its 3 products are anthranilate, pyruvate, and L-glutamate. (wikipedia.org)
  • Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. (uniprot.org)
  • The first committed enzyme in tryptophan biosynthesis is anthranilate synthase (AS). (genetics.org)
  • The larger of these (ORF2) encodes a 624 amino acid sequence similar to the overall sequence of the two subunits of anthranilate synthase. (microbiologyresearch.org)
  • Loss-of-function mutants of the Arabidopsis thaliana ACAULIS 5 ( ACL5 ) gene, which encodes spermine synthase, exhibit a severe dwarf phenotype. (biologists.org)
  • Okada M, Matsuzaki H, Shibuya I, Matsumoto K. Cloning, sequencing, and expression in Escherichia coli of the Bacillus subtilis gene for phosphatidylserine synthase. (labome.org)
  • With the purpose of generating a microbial strain for anthranilate production from glucose, E. coli W3110 trpD9923 , a mutant in the trpD gene that displays low anthranilate producing capacity, was characterized and modified using metabolic engineering strategies. (biomedcentral.com)
  • The results presented here, including the characterization of mutation in the trpD gene from strain W3110 trpD9923 and the development of a fermentation strategy, establish a step forward towards the future improvement of a sustainable process for anthranilate production. (biomedcentral.com)
  • Identification of Amino Acid Residues Involved in Feedback Inhibition of the Anthranilate Synthase in Escherichia coli", J. Biochem. (freepatentsonline.com)
  • In Escherichia coli anthranilate is synthesized from chorismate by anthranilate synthase (TrpED) and then converted to phosphoribosyl anthranilate by anthranilate phosphoribosyl transferase to continue the tryptophan biosynthetic pathway. (biomedcentral.com)
  • Other names in common use include anthranilate synthetase, chorismate lyase, and chorismate pyruvate-lyase (amino-accepting). (wikipedia.org)
  • However, neither enzyme displayed isochorismate pyruvate lyase (IPL) activity, which would enable these proteins to act as bifunctional SA synthases, i.e. to convert chorismate into SA. (portlandpress.com)
  • Other foods you could consider avoiding are: sugar, dairy products, deep-fried anthranilate synthase feedback inhibition food, meat, nut butters, and many others. (doctorsonfetalpain.org)
  • The regenerative properties of natural important anthranilate synthase feedback inhibition oils are believed to be particularly effective at treating mature skin and reducing the appearance of wrinkles and advantageous lines. (doctorsonfetalpain.org)
  • You'll be able to nonetheless exfoliate the pores and skin, which is able to anthranilate synthase feedback inhibition take away these lifeless cells. (doctorsonfetalpain.org)
  • Discover out what actual customers must say about acne free in 3 days anthranilate synthase feedback inhibition does it work and acne free in 3 days evaluate. (doctorsonfetalpain.org)
  • In E. coli citrate synthase, the enzyme involved in the condensation reaction initiating the Citric Acid Cycle is strongly inhibited by α-ketoglutarate feedback inhibition and can be inhibited by DPNH as well high concentrations of ATP. (wikipedia.org)
  • Indole-3-butyric acid promotes adventitious rooting in Arabidopsis thaliana thin cell layers by conversion into indole-3-acetic acid and stimulation of anthranilate synthase activity. (nih.gov)
  • Anthranilate is a common precursor of all these compounds and anthranilate synthase (AS) catalyzes the synthesis of anthranilate from chorismate and Gln. (plantphysiol.org)
  • Anthranilate synthase (AS) is a key enzyme in the synthesis of tryptophan (Trp), indole-3-acetic acid, and indole alkaloids. (plantphysiol.org)
  • As their last contribution to this field, his group answered the final mechanistic question in the pathway with the determination of the stereochemical course of EPSP synthase by total synthesis of the unstable tetrahedral intermediate. (berkeley.edu)
  • Anthranilate is an aromatic amine used industrially as an intermediate for the synthesis of dyes, perfumes, pharmaceuticals and other classes of products. (biomedcentral.com)
  • Chemical synthesis of anthranilate is an unsustainable process since it implies the use of nonrenewable benzene and the generation of toxic by-products. (biomedcentral.com)
  • This work constitutes the first example of a microbial system for the environmentally-compatible synthesis of anthranilate generated by metabolic engineering. (biomedcentral.com)
  • Anthranilate is an aromatic amine used as precursor for the synthesis of compounds having applications in the chemical, food and pharmaceutical industries. (biomedcentral.com)
  • Current anthranilate manufacture methods are based on chemical synthesis using precursors derived from petroleum, such as benzene. (biomedcentral.com)
  • In gram-negative bacteria, the most intensely studied quorum-sensing systems rely upon the interaction of N- acylhomoserine lactone (AHL) signal molecules, synthesized by LuxI-type AHL synthases, with LuxR-type transcriptional regulator proteins. (asm.org)
  • This finding suggested that the unusual cytoplasmic localization of the E. coli phosphatidylserine synthase plays a role in the regulation of the phospholipid polar headgroup composition in this organism. (labome.org)
  • The introduction of hydroxymandelate synthase (HmaS) from Amycolatopsis orientalis into E. coli led to a yield of 0.092 g/L S-MA. (biomedcentral.com)
  • Analysis of feedback-resistant anthranilate synthases from Saccharomyces cerevisiae. (ymdb.ca)
  • Extrinsic factors potassium chloride and glycerol induce thermostability in recombinant anthranilate synthase from Archaeoglobus fulgidus. (howard.edu)
  • Several microbial and plant species have the metabolic capacity to synthesize this aromatic compound, opening the possibility for generating sustainable technologies for anthranilate manufacture. (biomedcentral.com)
  • Carbon flow into the common aromatic pathway starts with the condensation of D-erythrose 4-phosphate (E4P) and phosphoenolpyruvate (PEP) to yield 3-deoxy-D- arabino -heptulosonate 7-phosphate (DAHP), in a reaction catalyzed by the enzyme DAHP synthase. (biomedcentral.com)
  • Novel expression vectors and constructs encoding a chloroplast transit peptide (CTP) operably linked to a monomeric anthranilate synthase are provided. (patents.com)
  • Anthranilate is an intermediate in the tryptophan biosynthetic pathway (Fig. 1 ). (biomedcentral.com)
  • The anthranilate synthase activity in S. venezuelae cell extracts was inhibited by tryptophan, although only at high concentrations of the amino acid. (microbiologyresearch.org)
  • Anthranilate synthase (AS) is a rate-limiting enzyme in the TIA pathway. (biomedcentral.com)
  • Differences in AS activity and anthranilate content among organs were small. (nii.ac.jp)
  • Subsequent reactions convert putrescine into spermidine and spermine, and these are catalyzed by spermidine and spermine synthases, which add propylamino groups from decarboxylated S -adenosylmethionine, catalyzed by S -adenosylmethionine decarboxylase (SAMDC) ( Pegg, 1988 ). (biologists.org)
  • When trp5-2wvc1 seedlings are grown on media supplemented with anthranilate metabolites, their roots wave like wild type. (nih.gov)
  • C. violaceum CV026 lacks the autoinducer synthase CviI and thus requires exogenous C6-HSL for violacein formation, which is QS-mediated [ 8 ]. (mdpi.com)
  • 1995. Le Haut-Saint-Laurent: un territoire en profonde mutation, échappant de plus en plus au contrôle naturel. (umontreal.ca)
  • TrpC (P00909): Phosphoribosylanthranilate isomerase domain first turns N-(5-phospho-β-D-ribosyl)anthranilate into 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate. (wikipedia.org)