Annexins: Family of calcium- and phospholipid-binding proteins which are structurally related and exhibit immunological cross-reactivity. Each member contains four homologous 70-kDa repeats. The annexins are differentially distributed in vertebrate tissues (and lower eukaryotes) and appear to be involved in MEMBRANE FUSION and SIGNAL TRANSDUCTION.Annexin A4: Protein of the annexin family originally isolated from the electric organ of the electric ray Torpedo marmorata. It has been found in a wide range of mammalian tissue where it is localized to the apical membrane of polarized EPITHELIAL CELLS.Annexin A2: A member of the annexin family that is a substrate for a tyrosine kinase, ONCOGENE PROTEIN PP60(V-SRC). Annexin A2 occurs as a 36-KDa monomer and in a 90-KDa complex containing two subunits of annexin A2 and two subunits of S100 FAMILY PROTEIN P11. The monomeric form of annexin A2 was formerly referred to as calpactin I heavy chain.Annexin A1: Protein of the annexin family exhibiting lipid interaction and steroid-inducibility.Annexin A3: A protein of the annexin family that catalyzes the conversion of 1-D-inositol 1,2-cyclic phosphate and water to 1-D-myo-inositol 1-phosphate.Annexin A5: A protein of the annexin family isolated from human PLACENTA and other tissues. It inhibits cytosolic PHOSPHOLIPASE A2, and displays anticoagulant activity.Annexin A7: An annexin family member that plays a role in MEMBRANE FUSION and signaling via VOLTAGE-DEPENDENT CALCIUM CHANNELS.Calcium-Binding Proteins: Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.Phosphatidylserines: Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a serine moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and serine and 2 moles of fatty acids.Cypriniformes: An order of fish with 26 families and over 3,000 species. This order includes the families CYPRINIDAE (minnows and CARPS), Cobitidae (loaches), and Catostomidae (suckers).Methylthiouracil: A thiourea antithyroid agent that inhibits the synthesis of thyroid hormone. It is used in the treatment of hyperthyroidism.Calcium: A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.Tetraphenylborate: An anionic compound that is used as a reagent for determination of potassium, ammonium, rubidium, and cesium ions. It also uncouples oxidative phosphorylation and forms complexes with biological materials, and is used in biological assays.Killifishes: Small oviparous fishes in the family Cyprinodontidae, usually striped or barred black. They are much used in mosquito control.Phospholipids: Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Dictyostelium: A genus of protozoa, formerly also considered a fungus. Its natural habitat is decaying forest leaves, where it feeds on bacteria. D. discoideum is the best-known species and is widely used in biomedical research.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.BooksPublishing: "The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.MEDLINE: The premier bibliographic database of the NATIONAL LIBRARY OF MEDICINE. MEDLINE® (MEDLARS Online) is the primary subset of PUBMED and can be searched on NLM's Web site in PubMed or the NLM Gateway. MEDLINE references are indexed with MEDICAL SUBJECT HEADINGS (MeSH).Serial Publications: Publications in any medium issued in successive parts bearing numerical or chronological designations and intended to be continued indefinitely. (ALA Glossary of Library and Information Science, 1983, p203)Biological Science Disciplines: All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.Abstracting and Indexing as Topic: Activities performed to identify concepts and aspects of published information and research reports.Annexin A7: An annexin family member that plays a role in MEMBRANE FUSION and signaling via VOLTAGE-DEPENDENT CALCIUM CHANNELS.Annexin A2: A member of the annexin family that is a substrate for a tyrosine kinase, ONCOGENE PROTEIN PP60(V-SRC). Annexin A2 occurs as a 36-KDa monomer and in a 90-KDa complex containing two subunits of annexin A2 and two subunits of S100 FAMILY PROTEIN P11. The monomeric form of annexin A2 was formerly referred to as calpactin I heavy chain.Annexin A5: A protein of the annexin family isolated from human PLACENTA and other tissues. It inhibits cytosolic PHOSPHOLIPASE A2, and displays anticoagulant activity.Annexin A1: Protein of the annexin family exhibiting lipid interaction and steroid-inducibility.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).beta 2-Glycoprotein I: A 44-kDa highly glycosylated plasma protein that binds phospholipids including CARDIOLIPIN; APOLIPOPROTEIN E RECEPTOR; membrane phospholipids, and other anionic phospholipid-containing moieties. It plays a role in coagulation and apoptotic processes. Formerly known as apolipoprotein H, it is an autoantigen in patients with ANTIPHOSPHOLIPID ANTIBODIES.Factor VII: Heat- and storage-stable plasma protein that is activated by tissue thromboplastin to form factor VIIa in the extrinsic pathway of blood coagulation. The activated form then catalyzes the activation of factor X to factor Xa.Antibody Specificity: The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Authorship: The profession of writing. Also the identity of the writer as the creator of a literary production.Knowledge Bases: Collections of facts, assumptions, beliefs, and heuristics that are used in combination with databases to achieve desired results, such as a diagnosis, an interpretation, or a solution to a problem (From McGraw Hill Dictionary of Scientific and Technical Terms, 6th ed).Annexin A6: Protein of the annexin family with a probable role in exocytotic and endocytotic membrane events.Editorial Policies: The guidelines and policy statements set forth by the editor(s) or editorial board of a publication.Scientific Misconduct: Intentional falsification of scientific data by presentation of fraudulent or incomplete or uncorroborated findings as scientific fact.Annexin A4: Protein of the annexin family originally isolated from the electric organ of the electric ray Torpedo marmorata. It has been found in a wide range of mammalian tissue where it is localized to the apical membrane of polarized EPITHELIAL CELLS.Telefacsimile: A telecommunication system combining the transmission of a document scanned at a transmitter, its reconstruction at a receiving station, and its duplication there by a copier.Trypsin: A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.Annexin A3: A protein of the annexin family that catalyzes the conversion of 1-D-inositol 1,2-cyclic phosphate and water to 1-D-myo-inositol 1-phosphate.Phosphatidylserines: Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a serine moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and serine and 2 moles of fatty acids.Liver Cirrhosis, Experimental: Experimentally induced chronic injuries to the parenchymal cells in the liver to achieve a model for LIVER CIRRHOSIS.Genome: The genetic complement of an organism, including all of its GENES, as represented in its DNA, or in some cases, its RNA.Databases, Genetic: Databases devoted to knowledge about specific genes and gene products.Regeneration: The physiological renewal, repair, or replacement of tissue.Calcium: A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.Liver Cirrhosis: Liver disease in which the normal microcirculation, the gross vascular anatomy, and the hepatic architecture have been variably destroyed and altered with fibrous septa surrounding regenerated or regenerating parenchymal nodules.Quantitative Trait Loci: Genetic loci associated with a QUANTITATIVE TRAIT.Holothuria: A genus of large SEA CUCUMBERS in the family Holothuriidae possessing thick body walls, a warty body surface, and microscopic ossicles.Antibodies, Viral: Immunoglobulins produced in response to VIRAL ANTIGENS.Antibodies, Bacterial: Immunoglobulins produced in a response to BACTERIAL ANTIGENS.Antibody Formation: The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.Exocytosis: Cellular release of material within membrane-limited vesicles by fusion of the vesicles with the CELL MEMBRANE.Chromaffin System: The cells of the body which stain with chromium salts. They occur along the sympathetic nerves, in the adrenal gland, and in various other organs.Cytoplasmic Granules: Condensed areas of cellular material that may be bounded by a membrane.Calcium-Binding Proteins: Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.Atrial Natriuretic Factor: A potent natriuretic and vasodilatory peptide or mixture of different-sized low molecular weight PEPTIDES derived from a common precursor and secreted mainly by the HEART ATRIUM. All these peptides share a sequence of about 20 AMINO ACIDS.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Foreskin: The double-layered skin fold that covers the GLANS PENIS, the head of the penis.Phospholipids: Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.Epidermal Growth Factor: A 6-kDa polypeptide growth factor initially discovered in mouse submaxillary glands. Human epidermal growth factor was originally isolated from urine based on its ability to inhibit gastric secretion and called urogastrone. Epidermal growth factor exerts a wide variety of biological effects including the promotion of proliferation and differentiation of mesenchymal and EPITHELIAL CELLS. It is synthesized as a transmembrane protein which can be cleaved to release a soluble active form.Fibroblasts: Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.Cell Count: The number of CELLS of a specific kind, usually measured per unit volume or area of sample.Ion Transport: The movement of ions across energy-transducing cell membranes. Transport can be active, passive or facilitated. Ions may travel by themselves (uniport), or as a group of two or more ions in the same (symport) or opposite (antiport) directions.Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.ArchivesDirectories as Topic: Lists of persons or organizations, systematically arranged, usually in alphabetic or classed order, giving address, affiliations, etc., for individuals, and giving address, officers, functions, and similar data for organizations. (ALA Glossary of Library and Information Science, 1983)Publications: Copies of a work or document distributed to the public by sale, rental, lease, or lending. (From ALA Glossary of Library and Information Science, 1983, p181)Social Sciences: Disciplines concerned with the interrelationships of individuals in a social environment including social organizations and institutions. Includes Sociology and Anthropology.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.Cell Line, Tumor: A cell line derived from cultured tumor cells.Caspase 3: A short pro-domain caspase that plays an effector role in APOPTOSIS. It is activated by INITIATOR CASPASES such as CASPASE 9. Isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Crystallization: The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Crystallography, X-Ray: The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Recombinant Proteins: Proteins prepared by recombinant DNA technology.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.NFATC Transcription Factors: A family of transcription factors characterized by the presence of highly conserved calcineurin- and DNA-binding domains. NFAT proteins are activated in the CYTOPLASM by the calcium-dependent phosphatase CALCINEURIN. They transduce calcium signals to the nucleus where they can interact with TRANSCRIPTION FACTOR AP-1 or NF-KAPPA B and initiate GENETIC TRANSCRIPTION of GENES involved in CELL DIFFERENTIATION and development. NFAT proteins stimulate T-CELL activation through the induction of IMMEDIATE-EARLY GENES such as INTERLEUKIN-2.Equidae: A family of hoofed MAMMALS consisting of HORSES, donkeys, and zebras. Members of this family are strict herbivores and can be classified as either browsers or grazers depending on how they feed.Calcineurin: A CALCIUM and CALMODULIN-dependent serine/threonine protein phosphatase that is composed of the calcineurin A catalytic subunit and the calcineurin B regulatory subunit. Calcineurin has been shown to dephosphorylate a number of phosphoproteins including HISTONES; MYOSIN LIGHT CHAIN; and the regulatory subunits of CAMP-DEPENDENT PROTEIN KINASES. It is involved in the regulation of signal transduction and is the target of an important class of immunophilin-immunosuppressive drug complexes.Creutzfeldt-Jakob Syndrome: A rare transmissible encephalopathy most prevalent between the ages of 50 and 70 years. Affected individuals may present with sleep disturbances, personality changes, ATAXIA; APHASIA, visual loss, weakness, muscle atrophy, MYOCLONUS, progressive dementia, and death within one year of disease onset. A familial form exhibiting autosomal dominant inheritance and a new variant CJD (potentially associated with ENCEPHALOPATHY, BOVINE SPONGIFORM) have been described. Pathological features include prominent cerebellar and cerebral cortical spongiform degeneration and the presence of PRIONS. (From N Engl J Med, 1998 Dec 31;339(27))Data Mining: Use of sophisticated analysis tools to sort through, organize, examine, and combine large sets of information.Propidium: Quaternary ammonium analog of ethidium; an intercalating dye with a specific affinity to certain forms of DNA and, used as diiodide, to separate them in density gradients; also forms fluorescent complexes with cholinesterase which it inhibits.Iodides: Inorganic binary compounds of iodine or the I- ion.Cat's Claw: A vine (Uncaria tomentosa) indigenous to the Amazon rainforest whose name is derived from its hook-like thorns. It contains oxindole alkaloids and glycosides and has many medicinal uses.Togaviridae: A family of RNA viruses, mainly arboviruses, consisting of two genera: ALPHAVIRUS (group A arboviruses), and RUBIVIRUS. Virions are spherical, 60-70 nm in diameter, with a lipoprotein envelope tightly applied to the icosahedral nucleocapsid.Arachis hypogaea: A plant species of the family FABACEAE that yields edible seeds, the familiar peanuts, which contain protein, oil and lectins.Peanut Agglutinin: Lectin purified from peanuts (ARACHIS HYPOGAEA). It binds to poorly differentiated cells and terminally differentiated cells and is used in cell separation techniques.Seeds: The encapsulated embryos of flowering plants. They are used as is or for animal feed because of the high content of concentrated nutrients like starches, proteins, and fats. Rapeseed, cottonseed, and sunflower seed are also produced for the oils (fats) they yield.Genome, Plant: The genetic complement of a plant (PLANTS) as represented in its DNA.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Microsatellite Repeats: A variety of simple repeat sequences that are distributed throughout the GENOME. They are characterized by a short repeat unit of 2-8 basepairs that is repeated up to 100 times. They are also known as short tandem repeats (STRs).Goats: Any of numerous agile, hollow-horned RUMINANTS of the genus Capra, in the family Bovidae, closely related to the SHEEP.Immunoglobulin M: A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.Enzyme-Linked Immunosorbent Assay: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.Blood Coagulation: The process of the interaction of BLOOD COAGULATION FACTORS that results in an insoluble FIBRIN clot.Hepatitis B virus: The type species of the genus ORTHOHEPADNAVIRUS which causes human HEPATITIS B and is also apparently a causal agent in human HEPATOCELLULAR CARCINOMA. The Dane particle is an intact hepatitis virion, named after its discoverer. Non-infectious spherical and tubular particles are also seen in the serum.Factor V: Heat- and storage-labile plasma glycoprotein which accelerates the conversion of prothrombin to thrombin in blood coagulation. Factor V accomplishes this by forming a complex with factor Xa, phospholipid, and calcium (prothrombinase complex). Deficiency of factor V leads to Owren's disease.Thromboplastin: Constituent composed of protein and phospholipid that is widely distributed in many tissues. It serves as a cofactor with factor VIIa to activate factor X in the extrinsic pathway of blood coagulation.Hepatitis B: INFLAMMATION of the LIVER in humans caused by a member of the ORTHOHEPADNAVIRUS genus, HEPATITIS B VIRUS. It is primarily transmitted by parenteral exposure, such as transfusion of contaminated blood or blood products, but can also be transmitted via sexual or intimate personal contact.Receptors, Formyl Peptide: A family of G-protein-coupled receptors that was originally identified by its ability to bind N-formyl peptides such as N-FORMYLMETHIONINE LEUCYL-PHENYLALANINE. Since N-formyl peptides are found in MITOCHONDRIA and BACTERIA, this class of receptors is believed to play a role in mediating cellular responses to cellular damage and bacterial invasion. However, non-formylated peptide ligands have also been found for this receptor class.Receptors, Lipoxin: Cell surface proteins that bind LIPOXINS with high affinity and trigger intracellular changes influencing the behavior of cells.Autacoids: A chemically diverse group of substances produced by various tissues in the body that cause slow contraction of smooth muscle; they have other intense but varied pharmacologic activities.Lipoxins: Trihydroxy derivatives of eicosanoic acids. They are primarily derived from arachidonic acid, however eicosapentaenoic acid derivatives also exist. Many of them are naturally occurring mediators of immune regulation.Leukocytes: White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES).Neutrophils: Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.Atherosclerosis: A thickening and loss of elasticity of the walls of ARTERIES that occurs with formation of ATHEROSCLEROTIC PLAQUES within the ARTERIAL INTIMA.Flow Cytometry: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.Myeloid Cells: The classes of BONE MARROW-derived blood cells in the monocytic series (MONOCYTES and their precursors) and granulocytic series (GRANULOCYTES and their precursors).Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Aprotinin: A single-chain polypeptide derived from bovine tissues consisting of 58 amino-acid residues. It is an inhibitor of proteolytic enzymes including CHYMOTRYPSIN; KALLIKREIN; PLASMIN; and TRYPSIN. It is used in the treatment of HEMORRHAGE associated with raised plasma concentrations of plasmin. It is also used to reduce blood loss and transfusion requirements in patients at high risk of major blood loss during and following open heart surgery with EXTRACORPOREAL CIRCULATION. (Reynolds JEF(Ed): Martindale: The Extra Pharmacopoeia (electronic version). Micromedex, Inc, Englewood, CO, 1995)Factor XIa: Activated form of factor XI. In the intrinsic pathway, Factor XI is activated to XIa by factor XIIa in the presence of cofactor HMWK; (HIGH MOLECULAR WEIGHT KININOGEN). Factor XIa then activates factor IX to factor IXa in the presence of calcium.Anticoagulants: Agents that prevent clotting.Factor Xa: Activated form of factor X that participates in both the intrinsic and extrinsic pathways of blood coagulation. It catalyzes the conversion of prothrombin to thrombin in conjunction with other cofactors.Amyloid beta-Protein Precursor: A single-pass type I membrane protein. It is cleaved by AMYLOID PRECURSOR PROTEIN SECRETASES to produce peptides of varying amino acid lengths. A 39-42 amino acid peptide, AMYLOID BETA-PEPTIDES is a principal component of the extracellular amyloid in SENILE PLAQUES.Partial Thromboplastin Time: The time required for the appearance of FIBRIN strands following the mixing of PLASMA with phospholipid platelet substitute (e.g., crude cephalins, soybean phosphatides). It is a test of the intrinsic pathway (factors VIII, IX, XI, and XII) and the common pathway (fibrinogen, prothrombin, factors V and X) of BLOOD COAGULATION. It is used as a screening test and to monitor HEPARIN therapy.HIV Protease Inhibitors: Inhibitors of HIV PROTEASE, an enzyme required for production of proteins needed for viral assembly.Protease Inhibitors: Compounds which inhibit or antagonize biosynthesis or actions of proteases (ENDOPEPTIDASES).Necrosis: The pathological process occurring in cells that are dying from irreparable injuries. It is caused by the progressive, uncontrolled action of degradative ENZYMES, leading to MITOCHONDRIAL SWELLING, nuclear flocculation, and cell lysis. It is distinct it from APOPTOSIS, which is a normal, regulated cellular process.Phagocytosis: The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES).Electronic Mail: Messages between computer users via COMPUTER COMMUNICATION NETWORKS. This feature duplicates most of the features of paper mail, such as forwarding, multiple copies, and attachments of images and other file types, but with a speed advantage. The term also refers to an individual message sent in this way.Receptor, PAR-1: A thrombin receptor subtype that couples to HETEROTRIMERIC GTP-BINDING PROTEINS resulting in the activation of a variety of signaling mechanisms including decreased intracellular CYCLIC AMP, increased TYPE C PHOSPHOLIPASES and increased PHOSPHOLIPASE A2.Receptor, PAR-2: A G-protein-coupled, proteinase-activated receptor that is expressed in a variety of tissues including ENDOTHELIUM; LEUKOCYTES; and the GASTROINTESTINAL TRACT. The receptor is activated by TRYPSIN, which cleaves off the N-terminal peptide from the receptor. The new N-terminal peptide is a cryptic ligand for the receptor. The uncleaved receptor can also be activated by the N-terminal peptide present on the activated THROMBIN RECEPTOR and by small synthetic peptides that contain the unmasked N-terminal sequence.Factor Va: Activated form of factor V. It is an essential cofactor for the activation of prothrombin catalyzed by factor Xa.Receptors, Proteinase-Activated: A class of receptors that are activated by the action of PROTEINASES. The most notable examples are the THROMBIN RECEPTORS. The receptors contain cryptic ligands that are exposed upon the selective proteolysis of specific N-terminal cleavage sites.Prothrombin: A plasma protein that is the inactive precursor of thrombin. It is converted to thrombin by a prothrombin activator complex consisting of factor Xa, factor V, phospholipid, and calcium ions. Deficiency of prothrombin leads to hypoprothrombinemia.Factor X: Storage-stable glycoprotein blood coagulation factor that can be activated to factor Xa by both the intrinsic and extrinsic pathways. A deficiency of factor X, sometimes called Stuart-Prower factor deficiency, may lead to a systemic coagulation disorder.Receptors, Thrombin: A family of proteinase-activated receptors that are specific for THROMBIN. They are found primarily on PLATELETS and on ENDOTHELIAL CELLS. Activation of thrombin receptors occurs through the proteolytic action of THROMBIN, which cleaves the N-terminal peptide from the receptor to reveal a new N-terminal peptide that is a cryptic ligand for the receptor. The receptors signal through HETEROTRIMERIC GTP-BINDING PROTEINS. Small synthetic peptides that contain the unmasked N-terminal peptide sequence can also activate the receptor in the absence of proteolytic activity.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.C-Reactive Protein: A plasma protein that circulates in increased amounts during inflammation and after tissue damage.Macrophages: The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)Phosphatidylcholines: Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a choline moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and choline and 2 moles of fatty acids.Lipoproteins, LDL: A class of lipoproteins of small size (18-25 nm) and light (1.019-1.063 g/ml) particles with a core composed mainly of CHOLESTEROL ESTERS and smaller amounts of TRIGLYCERIDES. The surface monolayer consists mostly of PHOSPHOLIPIDS, a single copy of APOLIPOPROTEIN B-100, and free cholesterol molecules. The main LDL function is to transport cholesterol and cholesterol esters to extrahepatic tissues.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Coronary Artery Disease: Pathological processes of CORONARY ARTERIES that may derive from a congenital abnormality, atherosclerotic, or non-atherosclerotic cause.Anti-Inflammatory Agents: Substances that reduce or suppress INFLAMMATION.Coronary Vessels: The veins and arteries of the HEART.Coronary Angiography: Radiography of the vascular system of the heart muscle after injection of a contrast medium.Coronary Disease: An imbalance between myocardial functional requirements and the capacity of the CORONARY VESSELS to supply sufficient blood flow. It is a form of MYOCARDIAL ISCHEMIA (insufficient blood supply to the heart muscle) caused by a decreased capacity of the coronary vessels.Coronary Artery Bypass: Surgical therapy of ischemic coronary artery disease achieved by grafting a section of saphenous vein, internal mammary artery, or other substitute between the aorta and the obstructed coronary artery distal to the obstructive lesion.Anti-Inflammatory Agents, Non-Steroidal: Anti-inflammatory agents that are non-steroidal in nature. In addition to anti-inflammatory actions, they have analgesic, antipyretic, and platelet-inhibitory actions.They act by blocking the synthesis of prostaglandins by inhibiting cyclooxygenase, which converts arachidonic acid to cyclic endoperoxides, precursors of prostaglandins. Inhibition of prostaglandin synthesis accounts for their analgesic, antipyretic, and platelet-inhibitory actions; other mechanisms may contribute to their anti-inflammatory effects.

Characterization of the calcyclin (S100A6) binding site of annexin XI-A by site-directed mutagenesis. (1/440)

Residues in annexin XI-A essential for binding of calcyclin (S100A6) were examined by site-directed mutagenesis. GST fusion proteins with the calcyclin binding site of annexin XI-A, GST-AXI 34-62 and GST-AXI 49-77 bound to calcyclin-Sepharose Ca2+-dependently. The mutants GST-AXI L52E, M55E, A56E and M59E lost the binding ability, whereas GST-AXI A57E retained the ability. These results demonstrate that the hydrophobic residues L52, M55, A56 and M59 on one side surface of the alpha-helix are critical for the binding. Assays with GST fusion proteins and synthesized peptides corresponding to the calcyclin binding site indicated that other regions around the calcyclin binding site are important to stabilize the conformation.  (+info)

GTPase activity and biochemical characterization of a recombinant cotton fiber annexin. (2/440)

A cDNA encoding annexin was isolated from a cotton (Gossypium hirsutum) fiber cDNA library. The cDNA was expressed in Escherichia coli, and the resultant recombinant protein was purified. We then investigated some biochemical properties of the recombinant annexin based on the current understanding of plant annexins. An "add-back experiment" was performed to study the effect of the recombinant annexin on beta-glucan synthase activity, but no effect was found. However, it was found that the recombinant annexin could display ATPase/GTPase activities. The recombinant annexin showed much higher GTPase than ATPase activity. Mg2+ was essential for these activities, whereas a high concentration of Ca2+ was inhibitory. A photolabeling assay showed that this annexin could bind GTP more specifically than ATP. The GTP-binding site on the annexin was mapped into the carboxyl-terminal fourth repeat of annexin from the photolabeling experiment using domain-deletion mutants of this annexin. Northern-blot analysis showed that the annexin gene was highly expressed in the elongation stages of cotton fiber differentiation, suggesting a role of this annexin in cell elongation.  (+info)

Transcription, biochemistry and localization of nematode annexins. (3/440)

The transcription of three annexin genes in the nematode, Caenorhabditis elegans, was detected by reverse transcriptase/polymerase chain reaction amplification of messenger RNAs. The highest level of expression was from the nex-1 gene, with lower levels detected for the nex-2 and nex-3 genes. The expression of nex-1 was reduced in the Dauer larval stage relative to the other annexins, correlating with the absence of the spermathecal valves, a major site of nex-1 protein localization. Recombinant nex-1 protein was expressed in yeast, isolated by calcium-dependent binding to acidic phospholipids, and its membrane binding and aggregating activities characterized using bovine chromaffin granules as a representative intracellular substrate. Binding to granule membranes was promoted by calcium with half-maximal binding seen at 630 microM calcium. Chromaffin granule aggregation was similarly promoted by the nex-1 protein at 630 microM calcium. This low sensitivity to calcium suggests the annexin can only be activated in vivo near the plasma membrane or other sources of calcium. Sequences including the nex-1 promoter were fused to the gene for green fluorescent protein and this construct was introduced into nematodes by microinjection. Examination of transgenic offspring revealed specific nex-1 promoter activity in the pharynx, the hypodermal cells, the vulva, and the spermathecal valve, locations in which the annexin may function in collagen secretion/deposition and membrane-membrane interactions. A sensitive anti-nex-1 antibody labelled with rhodamine was injected into body cavities of the nematode but did not detect extracellular nex-1 protein. Therefore, this annexin is apparently cytosolic and may function on the cytoplasmic side of the plasma membrane of the spermathecal valve to chaperon the folding of this membrane during the opening and closing of the valve.  (+info)

Annexin VII and annexin XI are tyrosine phosphorylated in peroxovanadate-treated dogs and in platelet-derived growth factor-treated rat vascular smooth muscle cells. (4/440)

The intraperitoneal administration of peroxovanadate results in the rapid accumulation of many tyrosine-phosphorylated proteins in the liver and kidney of treated animals. The availability of large pools of tyrosine-phosphorylated proteins derived from normal tissues facilitates the purification and identification of previously unknown targets for cellular tyrosine kinases. Using this procedure, we have thus far identified four proteins in the liver and kidney of peroxovanadate-treated dogs. Two of these, annexin VII and annexin XI, were novel and had not been previously reported to be substrates of tyrosine kinases while the remaining two, ezrin and clathrin, have been reported to be tyrosine phosphorylated in some cell culture systems. In the present study, isolated proteins were identified both by sequence analysis and immunological methods. Annexin VII and annexin XI are present in cultured rat vascular smooth muscle cells and both were tyrosine phosphorylated in response to a physiological ligand, platelet-derived growth factor-BB (PDGF-BB). Furthermore, the extent of tyrosine phosphorylation in response to PDGF-BB was augmented by the co-addition of peroxovanadate to cell cultures. In vitro phosphorylation assays showed that PDGF receptor, calcium-dependent tyrosine kinase (CADTK/Pyk-2), Src kinase, and epidermal growth factor receptor all were able to phosphorylate purified annexin VII and XI on tyrosine residues. These findings confirm the usefulness of phosphatase inhibition by peroxovanadate as a tool for identifying previously unknown physiological targets for cellular protein tyrosine kinases.  (+info)

Immunological development and cardiovascular function are normal in annexin VI null mutant mice. (5/440)

Annexins are calcium-binding proteins of unknown function but which are implicated in important cellular processes, including anticoagulation, ion flux regulation, calcium homeostasis, and endocytosis. To gain insight into the function of annexin VI, we performed targeted disruption of its gene in mice. Matings between heterozygous mice produced offspring with a normal Mendelian pattern of inheritance, indicating that the loss of annexin VI did not interfere with viability in utero. Mice lacking annexin VI reached sexual maturity at the same age as their normal littermates, and both males and females were fertile. Because of interest in the role of annexin VI in cardiovascular function, we examined heart rate and blood pressure in knockout and wild-type mice and found these to be identical in the two groups. Similarly, the cardiovascular responses of both sets of mice to septic shock were indistinguishable. We also examined components of the immune system and found no differences in thymic, splenic, or bone marrow lymphocyte levels between knockout and wild-type mice. This is the first study of annexin knockout mice, and the lack of a clear phenotype has broad implications for current views of annexin function.  (+info)

Annexins VII and XI are present in a human macrophage-like cell line. Differential translocation on FcR-mediated phagocytosis. (6/440)

We have studied the divalent cation-dependent association of proteins to subcellular fractions of human macrophage-like cells before and after FcR-mediated phagocytosis. Among these proteins we have identified annexins VII and XI for the first time in these cells, along with annexins I, III, and VI. Although all of these annexins are present in the cytosolic fraction, the extent of their association to membrane and phagosome fractions from resting and stimulated cells is variable. Annexin VII translocates from cytosolic to membrane fractions after phagocytic stimulation, along with annexin I, III, and VI. Annexins VII and XI are found associated with purified phagosomes along with I, III, and VI, and this association is greater after a 24-h chase period. Our results show differences in the intracellular distribution of different annexins in macrophage-like cells on phagocytosis. Annexins VII, VI, III, and I respond to particle ingestion by translocating to phagosomes and other cell membrane structures, whereas annexin XI translocates predominantly to phagosomes, suggesting dissimilarities in their function.  (+info)

Cellubrevin is present in the basolateral endocytic compartment of hepatocytes and follows the transcytotic pathway after IgA internalization. (7/440)

The endocytic compartment of polarized cells is organized in basolateral and apical endosomes plus those endocytic structures specialized in recycling and transcytosis, which are still poorly characterized. The complexity of the various populations of endosomes has been demonstrated by the exquisite repertoire of endogenous proteins. In this study we examined the distribution of cellubrevin in the endocytic compartment of hepatocytes, since its intracellular location and function in polarized cells are largely unknown. Highly purified rat liver endosomes were isolated from estradiol-treated rats, and the early/sorting endosomal fraction was further subfractionated in a multistep sucrose density gradient, and studied. Analysis of dissected endosomal fractions showed that cellubrevin was located in early/sorting endosomes, with Rab4, annexins II and VI, and transferrin receptor, but in a specific subpopulation of these early endosomes with the same density range as pIgA and Raf-1. Interestingly, only in those isolated endosomal fractions, endosomes enriched in transcytotic structures (of livers loaded with IgA), the polymeric immunoglobulin receptor specifically co-immunoprecipitated with cellubrevin. In addition, confocal and immuno-electron microscopy identification of cellubrevin in tubular structures underneath the sinusoidal plasma membrane together with the re-organization of cellubrevin, in the endocytic compartment, after the IgA loading, strongly suggest the involvement of cellubrevin in the transcytosis of pIgA.  (+info)

Annexin 24 from Capsicum annuum. X-ray structure and biochemical characterization. (8/440)

This work provides the first three-dimensional structure of a member of the plant annexin family and correlates these findings with biochemical properties of this protein. Annexin 24(Ca32) from Capsicum annuum was purified as a native protein from bell pepper and was also prepared by recombinant techniques. To overcome the problem of precipitation of the recombinant wild-type protein in crystallization trials, two mutants were designed. Whereas an N-terminal truncation mutant turned out to be an unstable protein, the N-terminal His-tagged annexin 24(Ca32) was crystallized, and the three-dimensional structure was determined by x-ray diffraction at 2. 8 A resolution. The structure refined to an R-factor of 0.216 adopts the typical annexin fold; the detailed structure, however, is different from non-plant annexins, especially in domains I and III and in the membrane binding loops on the convex side. Within the unit cell there are two molecules per asymmetric unit, which differ in conformation of the IAB-loop. Both conformers show Trp-35 on the surface. The loop-out conformation is stabilized by tight interactions of this tryptophan with residue side chains of a symmetry-related molecule and enforced by a bound sulfate. Characterization of this plant annexin using biophysical methods revealed calcium-dependent binding to phospholipid vesicles with preference for phosphatidylcholine over phosphatidylserine and magnesium-dependent phosphodiesterase activity in vitro as shown with adenosine triphosphate as the substrate. A comparative unfolding study of recombinant annexin 24(Ca32) wild type and of the His-tag fusion protein indicates higher stability of the latter. The effect of this N-terminal modification is also visible from CD spectra. Both proteins were subjected to a FURA-2-based calcium influx assay, which gave high influx rates for the wild-type but greatly reduced influx rates for the fusion protein. We therefore conclude that the N-terminal domain is indeed a major regulatory element modulating different annexin properties by allosteric mechanisms.  (+info)

*Annexin

However some annexins (Annexin A1, Annexin A2, and Annexin A5) have also been found outside the cellular environment, for ... Annexin, type I InterPro: IPR002388 Annexin, type II InterPro: IPR002389 Annexin, type III InterPro: IPR002390 Annexin, type IV ... In addition to annexin A-II, annexin A-XI has also been shown to organize cell membrane properties. Annexin A-XI is believed to ... The 310 amino acid annexin core has four annexin repeats, each composed of 5 alpha-helices. The exception is annexin A-VI that ...

*Annexin A9

... is a protein that in humans is encoded by the ANXA9 gene. The annexins are a family of calcium-dependent ... Goebeler V, Ruhe D, Gerke V, Rescher U (Jul 2003). "Atypical properties displayed by annexin A9, a novel member of the annexin ... Members of the annexin family contain 4 internal repeat domains, each of which includes a type II calcium-binding site. The ... "Entrez Gene: ANXA9 annexin A9". Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of ...

*Annexin A7

... is a protein that in humans is encoded by the ANXA7 gene. Annexin VII is a member of the annexin family of calcium- ... "The penta-EF-hand domain of ALG-2 interacts with amino-terminal domains of both annexin VII and annexin XI in a Ca2+-dependent ... "The penta-EF-hand domain of ALG-2 interacts with amino-terminal domains of both annexin VII and annexin XI in a Ca2+-dependent ... The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in ...

*Annexin Pharmaceuticals

The concept for the therapy is based on the anti-inflammatory properties of Annexin A5, a body own protective protein that acts ... Annexin Pharmaceuticals is a Swedish privately held biotech company developing new therapeutic approaches for inflammatory ... http://www.solidinfo.se/foretag/annexin-pharmaceuticals-ab/personer. ... "Annexin: Attenuating vascular inflammation" (PDF). BioCentury: A18. Retrieved 1 February 2015. ...

*Annexin A13

... is a protein that in humans is encoded by the ANXA13 gene. This gene encodes a member of the annexin family. ... "Comparative genetics and evolution of annexin A13 as the founder gene of vertebrate annexins". Molecular Biology and Evolution ... "Entrez Gene: ANXA13 annexin A13". Human ANXA13 genome location and ANXA13 gene details page in the UCSC Genome Browser. Wice BM ... "Apical membrane targeting of Nedd4 is mediated by an association of its C2 domain with annexin XIIIb". The Journal of Cell ...

*Annexin A3

"The high-resolution crystal structure of human annexin III shows subtle differences with annexin V". Biochemistry. 35 (6): 1740 ... Annexin A3 is a protein that in humans is encoded by the ANXA3 gene. It is abnormally expressed in fetuses of both IVF and ICSI ... "Entrez Gene: ANXA3 annexin A3". Zhang Y, Zhang YL, Feng C, et al. (September 2008). "Comparative proteomic analysis of human ... 2005). "Annexin A3 is a potential angiogenic mediator". Biochem. Biophys. Res. Commun. 337 (4): 1283-7. doi:10.1016/j.bbrc. ...

*Annexin A4

... is a protein that in humans is encoded by the ANXA4 gene. Annexin IV (ANX4) belongs to the annexin family of calcium ... "Entrez Gene: ANXA4 annexin A4". Human ANXA4 genome location and ANXA4 gene details page in the UCSC Genome Browser. Römisch J, ... Dreier R, Schmid KW, Gerke V, Riehemann K (Aug 1998). "Differential expression of annexins I, II and IV in human tissues: an ... Han EK, Tahir SK, Cherian SP, Collins N, Ng SC (Jul 2000). "Modulation of paclitaxel resistance by annexin IV in human cancer ...

*Annexin A6

... is a protein that in humans is encoded by the ANXA6 gene. Annexin VI belongs to a family of calcium-dependent ... It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Exon 21 of annexin VI is ... The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of ... Garbuglia M, Verzini M, Donato R (Sep 1998). "Annexin VI binds S100A1 and S100B and blocks the ability of S100A1 and S100B to ...

*Annexin A11

... is a protein that in humans is encoded by the ANXA11 gene. This gene encodes a member of the annexin family, a ... Farnaes L, Ditzel HJ (Aug 2003). "Dissecting the cellular functions of annexin XI using recombinant human annexin XI-specific ... "The penta-EF-hand domain of ALG-2 interacts with amino-terminal domains of both annexin VII and annexin XI in a Ca2+-dependent ... "The penta-EF-hand domain of ALG-2 interacts with amino-terminal domains of both annexin VII and annexin XI in a Ca2+-dependent ...

*Annexin A5

... (or annexin V) is a cellular protein in the annexin group. In flow cytometry, annexin V is commonly used to detect ... The annexin A5 affinity assay typically uses a conjugate of annexin V and a fluorescent or enzymatic label, biotin or other ... Annexin A5 showed upregulation in papillary thyroid carcinoma. Annexin A5 is used as a non-quantitative probe to detect cells ... Annexin A5 forms a shield around negatively charged phospholipid molecules. The formation of an annexin A5 shield blocks the ...

*Annexin A2

... also known as annexin II is a protein that in humans is encoded by the ANXA2 gene. Annexin 2 is involved in diverse ... Kwon M, MacLeod TJ, Zhang Y, Waisman DM (Jan 2005). "S100A10, annexin A2, and annexin a2 heterotetramer as candidate ... "Entrez Gene: ANXA2 annexin A2". Kling T, Ferrarese R, Ó hAilín D, Johansson P, Heiland DH, Dai F, Vasilikos I, Weyerbrock A, ... Annexin II is a pleiotropic protein meaning that its function is dependent on place and time in the body. The ANXA2 gene, ...

*Annexin A1

... , also known as lipocortin I, is a protein that is encoded by the ANXA1 gene in humans. Annexin A1 belongs to the ... Annexin A1 has also been shown to be associated with treatment resistance. ARID1A loss activates annexin A1 expression, which ... Annexin A1 has been of interest for use as a potential anticancer drug. Upon induction by modified NSAIDS and other potent anti ... Annexin A1 protects against DNA damage induced by heat in breast cancer cells, adding to the evidence that it has tumor ...

*Annexin A5 affinity assay

This clustering of annexin A5 on the membrane greatly increases the intensity of annexin A5 when labeled with a fluorescent or ... Labeling of annexin A5 with fluorescent or radioactive molecules makes it possible to detect binding of labeled annexin A5 to ... After binding to the phospholipid surface, annexin A5 assembles into a trimeric cluster. This trimer consists of three annexin ... Internalization amplifies additionally the intensity of the annexin A5 stained cell. Annexin A5 has been used to successively ...

*ANXA8L2

Annexin A8-like protein 2 is a protein that in humans is encoded by the ANXA8L2 gene. This gene encodes a member of the annexin ... 2006). "Annexin A8 is up-regulated during mouse mammary gland involution and predicts poor survival in breast cancer". Clin. ... Gerke V, Creutz CE, Moss SE (2005). "Annexins: linking Ca2+ signalling to membrane dynamics". Nat. Rev. Mol. Cell Biol. 6 (6): ... Goebeler V, Ruhe D, Gerke V, Rescher U (2006). "Annexin A8 displays unique phospholipid and F-actin binding properties". FEBS ...

*Niche (protein structural motif)

Proteins with regulatory cations often employ niches for metal binding (thrombin, Na+; annexin, Ca++; pyruvate dehydrogenase, ...

*S100A10

Annexin II, which is attracted to negatively charged phospholipids, binds to p11 at the Ca2+ binding site. In addition, Annexin ... Kwon M, MacLeod TJ, Zhang Y, Waisman DM (Jan 2005). "S100A10, annexin A2, and annexin a2 heterotetramer as candidate ... The p11 and annexin II complex is regulated by the phosphorylation of SerII on the annexin II molecule by protein kinase C (PKC ... Complexed with the annexin II, p11 binds receptor and channel proteins and guides them to the cell surface, resulting in ...

*Gunilla Carlsson

Board of Directors Annexin Pharmaceuticals. Members European Council on Foreign Relations. Governance Committee GAVI Alliance. ... In addition, Carlsson has held a variety of paid and unpaid positions, including the following: Annexin Pharmaceuticals, Member ...

*Mechanotransduction

Von Der Mark, K.; Mollenhauer, J. (1997). "Annexin V interactions with collagen". Cellular and Molecular Life Sciences (CMLS). ... annexin V (a collagen type II receptor), and integrin receptors (of which there exist several types on chondrocytes). Using the ... and annexin V". Journal of Orthopaedic Research. 19 (6): 1122-30. doi:10.1016/S0736-0266(01)00051-1. PMID 11781014. Pulai, ...

*S100A11

Seemann J, Weber K, Gerke V (August 1997). "Annexin I targets S100C to early endosomes". FEBS Letters. 413 (1): 185-90. doi: ... "Calcium-dependent binding of S100C to the N-terminal domain of annexin I". The Journal of Biological Chemistry. 271 (2): 719-25 ... the N-terminal part of annexin I". Structure. 8 (2): 175-84. doi:10.1016/S0969-2126(00)00093-9. PMID 10673436. Sakaguchi M, ...

*Sulfatide

... sulfatide binding to annexin V accelerates coagulation; third, sulfatide and P-selectin interactions expressed on platelets, ...

*Mary Ann McLaughlin

Annexin II and bleeding in acute promyelocytic leukemia. N Engl J Med 1991; 340: 994-1004. Mclaughlin M, Fuster V. Three ...

*Dysferlin

Lennon NJ, Kho A, Bacskai BJ, Perlmutter SL, Hyman BT, Brown RH (2003). "Dysferlin interacts with annexins A1 and A2 and ... including annexin and MG53. Exactly how dysferlin contributes to membrane resealing is not clear, but biochemical evidence ...

*Calmodulin

The other group, called annexins, bind calcium and phospholipid (e.g., lipocortin). Many other proteins bind calcium, although ...

*Antiphospholipid syndrome

Annexin A5 forms a shield around negatively charged phospholipid molecules, thus reducing their availability for coagulation. ... Thus, anti-annexin A5 antibodies increase phospholipid-dependent coagulation steps. The Lupus anticoagulant antibodies are ...

*Glucocorticoid

... annexin-1) synthesis. Lipocortin-1 both suppresses phospholipase A2, thereby blocking eicosanoid production, and inhibits ...
The 67 kDa calcimedin is a Ca2+-binding protein isolated from several muscle tissues. A recent report [Morse & Moore (1988) Biochem. J. 251, 171-174] indicated that the 67 kDa calcimedin is distinct from 67 kDa calelectrin, which is purified from various non-muscle cells. In the present study we have purified the 67 kDa protein from bovine aorta (i.e. 67 kDa calcimedin) and liver (i.e. 67 kDa calelectrin) and compared them by immunological and biochemical criteria. The aorta calcimedin is identical with the liver calelectrin by the following criteria. (1) The calcimedin co-electrophoresed with the calelectrin on SDS/5-15%-(w/v)-linear-gradient polyacrylamide gels. (2) The two proteins selectively cross-reacted with a chicken gizzard calcimedin antibody. (3) An antibody raised against the bovine aorta calcimedin also recognized the bovine liver calelectrin. (4) One-dimensional peptide maps of the two proteins revealed no significant difference. (5) The calcimedin appeared to have an amino acid ...
The 67 k calcimedin is a Ca2+-binding protein present in both muscle cells and peritoneal macrophages. Many tissues, including lymphoid tissues, liver and lymphocytes, have been shown to contain Ca2+-binding proteins of similar molecular size, such as the p67(67 kDa) calelectrin or the 68 kDa lymphocyte protein. We have tested affinity-purified antibodies raised to the smooth-muscle 67 k calcimedin in these several tissues and here report that the 67 k calcimedin is not detectable in liver, thymus, spleen or thymic lymphocytes. These findings support recent biochemical evidence, discussed here, suggesting that the 67 k calcimedin is a protein different from calelectrin and the 68 kDa lymphocyte protein. The more limited tissue distribution of the 67 k calcimedin, which includes muscle and macrophages, suggests that the 67 k calcimedin may function in Ca2+-mediated events special to these cell types. The affinity-purified antibodies to the 67 k calcimedin will be useful in obtaining information ...
TY - JOUR. T1 - Annexins family. T2 - Insights into their functions and potential role in pathogenesis of sarcoidosis. AU - Mirsaeidi, Mehdi. AU - Gidfar, Sanaz. AU - Vu, Ann. AU - Schraufnagel, Dean. PY - 2016. Y1 - 2016. N2 - Annexins are Ca2+-regulated phospholipid-binding proteins that play an important role in the cell life cycle, exocytosis, and apoptosis. Annexin A11 is one of the oldest vertebrate annexins that has a crucial role in sarcoidosis pathogenesis. The mechanism of effect in sarcoidosis granuloma cells may be due to alterations in apoptosis. Immune cells with a specific mutation at protein location 230 are resistant to apoptosis and consequently have continued effects on inflammation and progression of sarcoidosis. The mechanism of action of annexin A11 may be based upon alterations in delivering calcium to two different apoptosis pathways (caspase and P53).. AB - Annexins are Ca2+-regulated phospholipid-binding proteins that play an important role in the cell life cycle, ...
Based on sequence information from tryptic peptides an almost full-size cDNA coding for the human vascular anticoagulant was isolated from a placental cDNA library and sequenced. The coding region was cloned into an Escherichia coli expression vector and the protein expressed at high levels. The recombinant protein was purified and found to be indistinguishable from its natural counterpart in several biological assays ...
Primer recognition proteins (PRP) are cofactors for DNA polymerase alpha and may have a role in lagging-strand DNA replication. PRP is composed of two subunits, which we have previously identified as the protein-tyrosine kinase substrate annexin II and phosphoglycerate kinase (PGK). In this study, we have examined the physiological involvement of these proteins in DNA synthesis and cell proliferation. When exponentially growing human HeLa cells are exposed to antisense phosphorothioate oligodeoxynucleotides to annexin II, ongoing DNA synthesis is reduced. The extent of reduction with antisense oligodeoxynucleotide to PGK was much less than with the antisense annexin II oligodeoxynucleotide. Reductions in the labeling and mitotic indices of HeLa cell cultures are seen after exposure to antisense oligodeoxynucleotides. Flow cytometric analyses indicate that progression from S phase to G2 phase of the cycle is retarded by exposure of cells to the antisense oligodeoxynucleotides. Corresponding sense ...
ANXA2, a member of the annexin family, is a calcium-dependent phospholipid-binding protein that plays a role in the regulation of cellular growth and in signal transduction pathways. Its affinity for calcium is greatly enhanced by anionic phospholipids, and it binds two calcium ions with high affinity. ANXA2 may be involved in heat-stress response. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. It may also cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis.
Atrial natriuretic peptide is stored by atrial myocytes in secretory granules, known as atrial specific granules, and is released from these granules by exocytosis. We have isolated a group of atrial proteins by affinity chromatography that bind to atrial specific granules in a calcium-dependent manner. The two major proteins isolated (32.5 kd and 67 kd) are calcium-binding proteins and have been identified as annexins V and VI by immunoblotting with specific antisera. The calcium dependence of their binding to atrial specific granules has been characterized in vitro and indicates that this interaction takes place at micromolar levels of calcium. In addition, the group of proteins isolated includes another calcium-binding protein of 20 kd, as well as GTP-binding proteins of 22 to 26 kd. Membrane interactions during exocytosis are presumably mediated by the interaction of specific proteins with the granule membrane. The properties of the proteins described here, and their ability to bind to ...
Annexins are a structurally related family of Ca2+ binding proteins of undertermined biological function. Annexin I (also called lipocortin 1) is a substrate for the EGF-stimulated tyrosine kinase and is postulated to be involved in mitogenic signal transduction. To investigate further the involvement of lipocortin 1 in cell proliferation, we measured lipocortin 1 levels in normal diploid human foreskin fibroblasts (HFF) to determine whether its expression changed as a function of growth status. For comparison, the expression of annexin V (also called endonexin II) was measured in HFF cells. Endonexin II is a protein with similar Ca2+ and phospholipid binding properties as lipocortin 1, but it is not a substrate for tyrosine kinases. Quiescent HFF cell cultures were induced to proliferate by either subculture to lower cell density, EGF stimulation, or serum stimulation. In all three protocols, proliferating HFF cells contained three- to fourfold higher levels of lipocortin 1 and three- to ...
Plant annexins show distinct differences in comparison with their animal orthologues. In particular, the endonexin sequence, which is responsible for coordination of calcium ions in type II binding sites, is only partially conserved in plant annexins. The crystal structure of calcium-bound cotton annexin Gh1 was solved at 2.5Šresolution and shows three metal ions coordinated in the first and fourth repeat in types II and III binding sites. Although the protein has no detectable affinity for calcium in solution, in the presence of phospholipid vesicles, we determined a stoichiometry of four calcium ions per protein molecule using isothermal titration calorimetry. Further analysis of the crystal structure showed that binding of a fourth calcium ion is structurally possible in the DE loop of the first repeat. Data from this study are in agreement with the canonical membrane binding of annexins, which is facilitated by the convex surface associating with the phospholipid bilayer by a calcium ...
Annexin A1, also known as lipocortin I, is a protein that is encoded by the ANXA1 gene in humans. Annexin A1 belongs to the annexin family of Ca2+-dependent phospholipid-binding proteins that have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin A1 protein has an apparent relative molecular mass of 40 kDa with phospholipase A2 inhibitory activity. Glucocorticoids (such as budesonide, cortisol, and beclomethasone) are a class of endogenous or synthetic anti-inflammatory steroid hormones that bind to the glucocorticoid receptor (GR), which is present in almost every vertebrate animal cell. They are used in medicine to treat diseases caused by an overactive immune system, including allergies, asthma, autoimmune diseases, and sepsis. Because they suppress inflammatory pathways, long-term use of glucocorticoid drugs can lead to side-effects such as immunodeficiency and adrenal insufficiency. The main mechanism of ...
Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 has 45 to 59% identity with other members of its family and shares a similar size and exon-intron organization. Isolated from human placenta, ANX4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells ...
Membrane repair is essential to cell survival. In skeletal muscle, injury often associates with plasma membrane disruption. Additionally, muscular dystrophy is linked to mutations in genes that produce fragile membranes or reduce membrane repair. Methods to enhance repair and reduce susceptibility to injury could benefit muscle in both acute and chronic injury settings. Annexins are a family of membrane-associated Ca2+-binding proteins implicated in repair, and annexin A6 was previously identified as a genetic modifier of muscle injury and disease. Annexin A6 forms the repair cap over the site of membrane disruption. To elucidate how annexins facilitate repair, we visualized annexin cap formation during injury. We found that annexin cap size positively correlated with increasing Ca2+ concentrations. We also found that annexin overexpression promoted external blebs enriched in Ca2+ and correlated with a reduction of intracellular Ca2+ at the injury site. Annexin A6 overexpression reduced membrane ...
The discovery of an essential role for annexin 11 in cell division is unexpected when considered in the broader context of annexin biology. Although annexins are now firmly implicated in various cellular activities, including endocytosis, calcium signaling, and apoptosis, no member of this protein family has been demonstrated to function in the cell cycle. The membrane binding and vesicle fusogenic properties that define annexins in biochemical terms could provide clues as to the possible role of annexin 11 in the last stage of cytokinesis. Before cells can be completely separated in a process known as abscission, the actomyosin constriction ring at the cleavage furrow has to be disassembled and the central spindle must separate. During this series of events, it is likely that the plasma membrane must be tightly attached to the midbody microtubules for abscission to be successfully completed. CHO1 has previously been suggested to be responsible for this connection (Kuriyama et al., 2002), but ...
EZR (pT567) Antibody is a Rabbit Polyclonal antibody against EZR (pT567). The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein
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Sony NEX-7 high-end APS-C mirrorless camera first look: Sony has announced the much leaked NEX-7 enthusiast-targeted mirrorless interchangeable lens camera. | Everything Photographic
Av de läkemedel som intas kan kroppen endast ta upp en liten del medan en stor del utsöndras igen, framförallt via urinen. Då avloppsvatten kommer in till avloppsreningsverken renas det genom mekaniska, kemiska och biologiska processer men dagens avloppsreningsverk är inte designade för att ta hand om och bryta ner läkemedelsrester och andra organiska föreningar från till exempel tvättmedel, färgrester etc. Beroende på de olika substansernas kemiska och fysikaliska egenskaper följer de antingen med det färdigrenade utloppsvattnet, som lämnar avloppsreningsverket, eller så hamnar de i slammet som bildas av sedimenterande partiklar under reningen. Slammet rötas och avvattnas och kan sedan bland annat användas som gödningsmedel på jordbruksmark.. I denna studie undersöktes huruvida samkompostering av avloppsslam och annat organiskt material kan höja slammets status som gödselmedel med avseende på minskad lukt, näringsinnehåll och minskad förekomst av läkemedelsrester och ...
Creutz, C. E.; Moss, S.; Edwardson, J. M.; Hide, I.; Gomperts, B.; Schiavo, G.; Nevalainen, L.; von Rueden, L.; Fletcher, L.; Bartels, F. et al.; Waschulewski, I.; Buccione, R.; Pinxteren, J.; Magnus, C.: Differential recognition of secretory vesicles by annexins. Biochemical and Biophysical Research Communications 184 (1), pp. 347 - 352 (1992 ...
C Mount Lens to NEX E Mount adapterDescription:With this adapter, you can use C- Mount Lenses on NEX E mount camera. Disphragm control of the lens
Jag har huvudsakligen intresserat mig för omställningen vid födelsen såsom stressen att födas, plötslig spädbarnsdöd och hjärnans utveckling. Under de senaste åren har jag ägnat mig åt frågan om hur och när medvetandet uppkommer bland annat genom att studera hjärnans spontana aktivitet med funktionell magnetkamerateknik. Hjärnans nätverk kan felkopplas hos extremt för tidigt födda barn och vara en orsak till att de drabbas av autismliknande tillstånd.. ...
DAZED & CONFUSED (UK) i Tidningsarkivet. Ett digitalt arkiv för svenska tidningar och tidskrifter. Här finns bland annat omslag och innehållstexter för DAZED & CONFUSED (UK).
SMURFARNA - SPORTPYSSLA MED SMURFARNA i Tidningsarkivet. Ett digitalt arkiv för svenska tidningar och tidskrifter. Här finns bland annat omslag och innehållstexter för SMURFARNA - SPORTPYSSLA MED SMURFARNA.
I arbetet med denna studie har jag genom intervjuer och enkäter undersökt vad utlandsfödda personer som har barn i den svenska skolan, har för erfarenheter och åsikter om utvecklingssamtal, både i hemlandet och härifrån Sverige. Intervjupersonerna har bland annat tillfrågats om huruvida de har varit med om utvecklingssamtal under sin egen studietid i hemlandet. De har också tillfrågats om vad de har för tankar kring de utvecklingssamtal de varit med om under sina barns studietid och vad de har för syn på den svenska skolan. Resultatet blev övervägande positivt, även om vissa hade en del negativa erfarenheter. Intervjupersonerna har vidare tillfrågats om sin syn på den svenska skolan.. ...
TY - JOUR. T1 - The membrane phospholipid binding protein annexin A2 promotes phagocytosis and nonlytic exocytosis of cryptococcus neoformans and impacts survival in fungal infection. AU - Stukes, Sabriya. AU - Coelho, Carolina. AU - Rivera, Johanna. AU - Jedlicka, Anne E.. AU - Hajjar, Katherine A.. AU - Casadevall, Arturo. PY - 2016/8/15. Y1 - 2016/8/15. N2 - Cryptococcus neoformans is a fungal pathogen with a unique intracellular pathogenic strategy that includes nonlytic exocytosis, a phenomenon whereby fungal cells are expunged from macrophages without lysing the host cell. The exact mechanism and specific proteins involved in this process have yet to be completely defined. Using murine macrophages deficient in the membrane phospholipid binding protein, annexin A2 (ANXA2), we observed a significant decrease in both phagocytosis of yeast cells and the frequency of nonlytic exocytosis. Cryptococcal cells isolated from Anxa2-deficient (Anxa2-/-) bone marrow-derived macrophages and lung ...
Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. The alternative splicing event is tissue specific and the mRNA containing the cassette exon is prevalent in brain, heart and skeletal muscle. The transcripts also differ in their 3-non coding regions by the use of two alternative poly(A) signals. Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, ...
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis. Alternatively spliced transcript variants have been described. [provided by RefSeq, Aug 2010 ...
Sigma-Aldrich offers abstracts and full-text articles by [MeiJing He, XinLei Yang, ShunLi Cui, GuoJun Mu, MingYu Hou, HuanYing Chen, LiFeng Liu].
Looking for online definition of lipocortin in the Medical Dictionary? lipocortin explanation free. What is lipocortin? Meaning of lipocortin medical term. What does lipocortin mean?
Helen Christians research interests are in the mechanisms of steroid hormone regulation of the pituitary gland, in particular the role of Annexin 1. The Annexins are a well-conserved super-family of structurally related Ca2+ - and phospholipids-binding proteins with wide-ranging functions in health and disease. Annexin 1 is a 37kD protein that is induced by glucocorticoids and mediates glucocorticoid action within the host defence and neuroendocrine systems. Glucocorticoids regulate the synthesis, phosphorylation and cellular disposition of annexin 1, and annexin 1 is implicated in the regulation by these important drugs of pituitary function, the control of cell growth and signal transduction. Most recently her group have characterized a novel secretory pathway of annexin 1, a protein which lacks a signal sequence, involving a member of the ATP binding cassette transporter family, ABCA1. This is the first time the secretion mechanism of an annexin family member has been determined and the ...
A idea behind this research is: if we know what parts of the bacteria stimulate healing, perhaps doctors can deliver that material, or something very close, to patients directly to treat intestinal diseases such as Crohns or ulcerative colitis.. This idea has advanced experimentally, as demonstrated by two papers from Jones and Neish’s frequent collaborator, Asma Nusrat, who recently moved from Emory to the University of Michigan. This team had shown that a protein produced by human intestinal cells called annexin A1 activates ROS, acting through the same N-formyl peptide receptors that bacteria do.. Nusrat told me Friday her team began investigating annexins a decade ago at Emory, and it was fortuitous that Neish was working on beneficial bacteria right down the hall, since it is now apparent that annexin A1 and the bacteria are activating the same molecular signals. (Did you know there is an entire conference devoted to annexins? I didnt until a few days ago.). In a ...
PGK1 an apparent multifunctional protein. A glycolytic enzyme and a polymerase alpha cofactor protein (primer recognition protein). Note: This description may include information from UniProtKB ...
From Sony literature) Sony adds superb responsiveness, image quality, and creative possibilities to its line of E-mount interchangeable lens cameras with the new α NEX-5N model. Housed in a rugged, light magnesium alloy body, with an intuitive touch-screen interface, the camera is just 23.3mm at its slimmest point.. "The NEX-5N camera extends the sleek, minimalist design of the acclaimed NEX-5," said Ernesto Bravo, senior business manager of the alpha group at Sony Electronics. "But its also a giant step forward from its predecessor in terms of still image and HD video quality, Auto Focus and speed-of-capture performance.". Response is accelerated even further by a release time lag of approximately 20 milliseconds, the fastest of any interchangeable lens digital camera. Combined with an improved autofocus (AF) algorithm and more efficient image processing, this results in an AF that is significantly faster than the current NEX-5 model.. The cameras Exmor™ APS HD CMOS sensor with 16.1 ...
From Sony literature) Sony is taking professional quality imaging and artistic shooting options to new levels with the introduction of the new α NEX-7 camera. Styled in magnesium alloy for strength and designed with a unique blend of classic and modern inspiration, the new compact interchangeable lens model weighs only 10.3 ounces (291g).. "For Sony, the new NEX-7 represents innovation in its purest form," said Kelly Davis, vice president of digital imaging at Sony Electronics. "Speed and style meet performance and control, creating a shooting experience unlike anything in market today.". The NEX-7 camera features a new Exmor™ APS HD CMOS sensor with 24.3 effective megapixel resolution -- highest in its class -- that complements the latest generation of Sonys BIONZ® image processing engine.. This unique combination of sensor and processor technology delivers flawless image and HD video quality with very low noise and exceptionally fast shooting responses. An ultra-high sensitivity range ...
Wells, D., Wells, K., Liu, K., Hannon, Robert, Croxtall, Jamie D., Damazo, A.S., Oliani, Sonia M., Getting, Stephen J., Parente, Luca, Paul-Clark, Mark, Yona, Simon, Gavins, Felicity N.E., Martin, Joanne, Christian, Helen C., Cover, Patricia O., John, C.D., Solito, Egle, Morris, John F., Perretti, Mauro, Buckingham, Julia C. and Flower, Roderick J. (2004) The annexin 1-/- mouse: phenotypic studies. Annexins, 1 (2). pp. 109-120. ISSN 1547-6294 ...
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Grammatik är ett ämne som anses framkalla negativa känslor och många människor minns sin grammatikundervisning som tråkig och svår. Grammatik är dock ett centralt ämne för oss språkkonsulter och nödvändigt för att vi ska kunna utföra vårt arbete. Syftet med den här undersökningen har därför varit att undersöka vilka attityder till grammatik som människor egentligen har. Frågeställningarna handlar om hur människor definierar grammatik, vilken uppfattning de har av grammatik samt vilken användning de ser med grammatik. Metoden för att söka svar på dessa frågor har varit fokusgruppssamtal där studerande och arbetande människor tillsammans fått tala om grammatik. Resultaten visar att det finns många negativa kommentarer om grammatikundervisning, men också att grammatik anses viktigt för bland annat förståelse och för hur kompetent man kan uppfattas av andra människor. Slutsatserna som dragits utifrån resultaten är att människor inte är så negativa till ...
I syfte att belysa de yngsta barnen med annat modersmål än svenska i förskolan har intervjuer av pedagoger samt observationer i verksamheten utförts. Undersökningens fokus har lagts vid enskilda pedagogers åsikter och erfarenheter. Studien fokuserar på hur kommunikationen ser ut mellan barn och pedagog, hur deras modersmål bevaras och hur språkinlärningen sker. Barnens kultur och språk kommer i fokus då dessa är direkt sammankopplade till identiteten och dess utveckling. Samtliga respondenter betonar hur viktigt det är att vara tydlig, med både ord och kroppsspråk. När det handlar om att lära sig ett helt nytt språk måste tydligheten vara densamma som med ett litet barn som lär sig det verbala språket från allra första början. Resultaten visar även att pedagogerna är positivt inställda till att arbeta med flerspråkiga barn men att det många gånger saknas kunskap och resurser. Barnens modersmål fick sällan komma fram eller tränas i förskolans verksamhet. Detta ...
A scientific resource for the PH domain containing information on structure, function, and domain binding to phospholipids during protein trafficking and cell signaling.
Alpha-giardins constitute the annexin proteome (group E annexins) in the intestinal protozoan parasite Giardia and, as such, represent the evolutionary oldest eukaryotic annexins. The dominance of alpha-giardins in the cytoskeleton of Giardiawith its greatly reduced actin content emphasises the importance of the alpha-giardins for the structural integrity of the parasite, which is particularly critical in the transformation stage between cyst and trophozoite. In this study, we report the crystal structures of the apo- and calcium-bound forms of a1-giardin, a protein localised to the plasma membrane of Giardia trophozoites that has recently been identified as a vaccine target. The calcium-bound crystal structure of a1-giardin revealed the presence of a type III site in the first repeat as known from other annexin structures, as well as a novel calcium binding site situated between repeats I and IV. By means of comparison, the crystal structures of three different alpha-giardins known to date ...
The Annexin family of calcium-binding proteins is composed of al least thirteen mammalian genes (Annexin A1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. The Annexin family
It is thought that the involvement of common mediators such as corticotrophin releasing hormone (CRR), cytokines, prostaglandins (PGs), pituitary peptides and glucocorticoids allows the integration of immune and neuroendocrine activity during the acute phase response (APR) to injury and infection. Glucocorticoids are the major inhibitory component within this complex system, which has been termed the immunehypothalamic- pituitary-adrenal (HPA) axis. In the past, lipocortin-1 (LC-1) has been implicated as a "second messenger" of glucocorticoid actions. The present study explored this potential role for LC-l in relation to the immune-HPA axis ...
Objectives: Diabetic patients frequently suffer from chronic infections and inflammation. At the same time, diabetes mellitus is a common comorbidity in Staphylococcus aureus infections associated with a poorer outcome. Our goal was to study the role of neutrophils in S. aureus infections complicated by diabetes.. Methods: We used age-matched diabetic and non-diabetic NOD mice challenged with S. aureus i.p. as an animal model of systemic infection to study neutrophil apoptosis during infection (as measured by nuclear condensation, Annexin binding and TUNEL staining). Fluorescent labeled neutrophils were used to investigate neutrophil clearance by macrophages and intracellular cytokine staining to study the production of proinflammatory cytokines.. Results: Neutrophils isolated from diabetic mice infected with S. aureus displayed enhanced viability and a 11-35% lower rate of apoptosis in all assays used. Similarly, neutrophils from diabetic mice stimulated with S. aureus in vitro underwent ...
Phospholipid-binding protein EhC2A mediates calcium-dependent translocation of transcription factor URE3-BP to the plasma membrane of Entamoeba histolytica ...
Den högre utbildningen, och då inte minst undervisningen i akademiskt skrivande, står inför en stor utmaning, nämligen att försöka lösa två till synes motstridiga uppgifter: att se till att studenterna klarar högskolans utbildningsmål, som t.ex. att kunna skriva ett självständigt arbete/examensarbete, och att förbereda dem för det moderna arbetslivets mångskiftande krav på den skrivna kommunikationens område. I artikeln argumenterar jag för att det går att förena dessa båda uppgifter, och jag ger också ett exempel på och diskuterar hur det skulle kunna gå till. Centralt är bland annat att varje ämnesdisciplin utformar sin egen variant av skrivundervisning, att skrivkompetens ses som en förhållandevis komplex uppsättning av kunskaper och färdigheter samt att skrivundervisningen bedrivs på ett explicit och genomskinligt sätt så att studenterna ges möjlighet att tillägna sig ett metaperspektiv på skrivande.. ...
Storlek har varit en av de intressantaste aspekterna av dinosaurieforskningen för allmänheten. Dinosaurier visar en enorm variation i storlek, vissa var (och är än idag i form av fåglarna) mycket små, medan andra kunde nå enorma storlekar.. Vetenskapsmän kommer troligtvis aldrig vara helt säkra på vilken den största eller minsta dinosaurien var på grund av en rad anledningar, bland annat att endast en liten marginal av djur fossiliseras, att mycket av det som fossiliseras troligen inte har hittats än och att mycket av det man hittar är inkomplett. Ofta måste skelett rekonstrueras baserat på nära släktingar, vilket inte är något fullständigt säkert.. Denna artikel listar de största till de minsta dinosaurierna i respektive grupper sorterade efter längd och vikt. De stora marginalerna tas med för att visa den grad av osäkerhet som kan uppstå när man försöker beräkna storleken hos ett fossiliserat djur, i synnerhet med vikten då det är betydligt lättare att mäta ...
Ramcon erbjuder olika instrument och tillbehör för material- och partikelkaraktärisering bland annat för analys av partikelstorlek, storleksdistribution, ljusspridning, zeta-potential, XRF, XRD, ångsorption, adsorption / desorption, korrosionsprovning, saltspraytest och kristallinitet ...
花月正春风/刘墉作/绢本设色/工笔/76.5x46.5CM/2013 即使 天堂 是不愁吃穿3不必工作3永永远远活着3 那活着有什么意义3 正因为有苦恼3才有解决的快乐; 正因为有病痛3才有痊愈的欢喜4 正因为有忧愁3才有忘忧的美好4 正因为有今天的不足3 ...
To evaluate the safety and efficacy of glucarpidase in patients with impaired methotrexate (MTX) clearance owing to MTX-induced renal failure following
Glucocorticoid steroids such as prednisone are prescribed for chronic muscle conditions such as Duchenne muscular dystrophy, where their use is associated with prolonged ambulation. The positive effects of chronic steroid treatment in muscular dystrophy are paradoxical because these steroids are also known to trigger muscle atrophy. Chronic steroid use usually involves once-daily dosing, although weekly dosing in children has been suggested for its reduced side effects on behavior. In this work, we tested steroid dosing in mice and found that a single pulse of glucocorticoid steroids improved sarcolemmal repair through increased expression of annexins A1 and A6, which mediate myofiber repair. This increased expression was dependent on glucocorticoid response elements upstream of annexins and was reinforced by the expression of forkhead box O1 (FOXO1). We compared weekly versus daily steroid treatment in mouse models of acute muscle injury and in muscular dystrophy and determined that both ...
|p|Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry
Animals. The WT mice on a 129T2/SvEmsJ background were used for studies involving acute injury protocols. The mdx mice on a DBA/2J background were obtained from The Jackson Laboratory (stock 013141) and interbred. Male mice were used for reported experiments. Mice were housed in a specific pathogen-free facility.. Plasmids. GFP-tagged annexins A1 and A6 were described previously (14, 16). The 3′ UTR of Anxa6 was PCR cloned from cDNA from 129T2/SvEmsJ skeletal muscle and ligated into the pmirGlo plasmid (catalog 1330, Promega) using the SacI-XhoI sites; the miR-383-binding site (5′-CUGCAUUCAACUCUGAUC-3′) was ablated by means of a Phusion Site-Directed Mutagenesis Kit (catalog F541, Thermo Scientific). Luciferase plasmids containing promoter fragments were obtained by cloning genomic sequences into pGL4.23 (E8411, Promega) using the KpnI-XhoI sites. Cloned genomic sequences from 129T2/SvEmsJ mice were used for GRE/FOXO site analysis (Supplemental Table 2).. Electroporation, myofiber ...
Bought the Sony NEX-3N from Amazon when they have a lightning deal on the 13th January 2014 and hence it cost only US$289.99 (S$370)! HopShopGo received the package on the 18th January 2014 with a chargeable weight of 3KG, but HopShopGo offers a repack option for a slight fee and hence it cuts down the …
Användandet av så kallade semiprofessionella resurser i räddningsinsatser har blivit allt vanligare i Sverige. En semiprofessionell är en person som fått utökade arbetsuppgifter inom respons och räddning inom ramen för sitt ordinarie yrke. Det kanske mest kända exemplet är väktare som responderar på den kommunala räddningstjänstens ärenden.. I det projekt som avrapporteras här, är syftet att undersöka vilka yrkesgrupper som skulle passa bra som semiprofessionella, och vad som krävs för att de effektivt ska kunna utföra de nya arbetsuppgifterna, bland annat i form av utbildning och utrustning. Vidare syftar projektet till att utvärdera vilken samhällsnytta semiprofessionella kan bidra med. Som studieobjekt används Norrköpings kommun, och via en aktionsforskningsinspirerad metodansats är målet att projektresultaten ska kunna bidra till en ökad säkerhet och trygghet i kommunen.. En kombination av kvalitativa och kvantitativa metoder används för att uppnå syftet. ...
Storhjärnan (även cerebrum eller telencephalon) är en del av hjärnan och därmed det centrala nervsystemet hos ryggradsdjur. Endast hos de större däggdjuren dominerar den storleksmässigt och förtjänar benämningen storhjärna. ¶ Hos människan upptar storhjärnan 90 % av hjärnvolymen, och det är detta som ger människan en förmåga till abstrakt tänkande som skiljer sig från övriga djur. Här finns intellekt, konstnärlighet och abstrakt språk. Detta finns lagrat i storhjärnans yttersta lager, i hjärnbarken (cortex). Barken är omkring tre till fem millimeter tjock och den är kraftigt veckad så att dess yta (1,5 m2) får plats under skallbenen. Barken består av grå substans och under denna finns vit substans. Djupt inuti storhjärnan finns även inslag av grå substans, bland annat de basala ganglierna. Förenklat är grå substans cellkärnor medan den vita substansen är utskott som omges av en särskilt form av fett, myelin. Den vita substansen sköter om ...
Darkness on the Edge of Town är ett musikalbum av Bruce Springsteen, utgivet av skivbolaget Columbia Records den 2 juni 1978. Albumet var det första efter att Springsteen löst en lång tvist med sitt skivbolag.. Till skillnad från det livsbejakande albumet Born to Run innehåller Darkness on the Edge of Town mörka och dystra skildringar av bland annat Springsteens förhållande till sin far, exempelvis i "Adam Raised a Cain" och "Factory." När det gäller låtordningen på den ursprungliga LP:n fortsatte Springsteen sin "fyra hörn"-modell från "Born to Run". Låtarna i början på varje sida ("Badlands" och "The Promised Land") var brutala rop för att övervinna omständigheter, medan låtarna som slutar varje sida ("Racing in the Street", "Darkness on the Edge of Town") var sorgsna sånger där allt hopp försvunnit.. Albumet sålde sämre än föregångaren, även om det inte sålde dåligt, med en femteplats på Billboards albumlista. Det misslyckades dock med att få fram någon ...
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Because there is little knowledge in the areas of stereocilia development, maintenance, and function in the hearing system, I decided to pursue a proteomics-based approach to discover proteins that play a role in stereocilia function. I employed a modified "twist-off" technique to isolate hair bundle proteins, and I developed a method to purify proteins and to process them for analysis using multi-dimensional protein identification technology (MudPIT). The MudPIT analysis yielded a substantial list of proteins. I verified the presence of 21 out of 34 (62%) existing proteins known to be present in stereocilia. This provided strong evidence that my proteomics approach was efficient in identifying hair bundle proteins. Next, I selected three proteins and localized them to murine cochlear stereocilia. StarD10, a putative phospholipid binding protein, was detectable along the shaft of stereocilia. Nebulin, a putative F-actin regulator, was located toward the base of stereocilia. Finally, twinfilin 2, ...
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Two-dimensional crystals of annexin-V bound to lipid layers containing dioleoylphosphatidylserine have been obtained in the presence of Ca2+. The crystals diffract to 20 A resolution and have the symmetry of the plane group p3 (unit cell dimensions: a = b = 94 A, gamma = 120 degrees). Electron image analysis revealed that the crystals are composed of trimers of annexin-V forming triskelion-like motifs. Each annexin-V molecule has a characteristic elongated shape, about 65 A by 20 A, when observed perpendicularly to the crystal plane. It is composed of two staggered domains of similar size, about 40 A by 20 A. Both domains are made of two sub-domains. The present data suggest that the four resolved sub-domains represent the folding units corresponding to the four 70 amino acid repeating segments characteristic of all annexins.
The N-terminal EGF domain has been shown to at least in part be responsible for binding tissue factor.[6] Wilkinson et al. conclude that residues 88 to 109 of the second EGF domain mediate binding to platelets and assembly of the factor X activating complex.[7] The structures of all four domains have been solved. A structure of the two EGF domains and the trypsin-like domain was determined for the pig protein.[8] The structure of the Gla domain, which is responsible for Ca(II)-dependent phospholipid binding, was also determined by NMR.[9] Several structures of super active mutants have been solved,[10] which reveal the nature of factor IX activation by other proteins in the clotting cascade. ...
Supplementary Materials Supplemental Data supp_285_9_6781__index. 1A binds to PI(4 preferentially,5)P2-containing liposomes and that PI(4,5)P2 antagonizes the ability of Coronin 1A to disassemble actin filament branches, indicating a spatiotemporal regulation of Coronin 1A via a direct interaction with the plasma membrane lipid. Collectively, our proteomics data provide a list of potential acidic phospholipid-binding protein candidates ranging from the actin PF 429242 cost regulatory proteins to translational regulators. and structural studies showed that actin-binding sites of these ABPs are overlapped with PI(4,5)P2-binding sites and that the activity of ABPs is inhibited by PI(4,5)P2 (6,C8). Therefore, the activity of ABPs have thought to be inhibited by PI(4,5)P2 binding that occludes F-actin-binding sites and to be activated by PI(4,5)P2 hydrolysis when phospholipase C (PLC) PF 429242 cost is activated by receptor stimulation (5). Indeed, recent study have reported that Cofilin, an ...
MODEL AL-1216 AL-1225 DIGITAL LASER COPIER OPERATION MANUAL Page INTRODUCTION 4 SETTING UP THE UNIT 7 LOADING PAPER MAKING COPIES SPECIAL FUNCTION MAINTENANCE AL-1216 AL-1225 TROUBLESHOOTING THE UNIT OPTIONAL EQUIPMENT (AL-12PK) APPENDIX 14 19 26 29 33 40 69 CLASS 1 LASER PRODUCT LASER KLASSE 1 LUOKAN 1 LASERLAITE KLASS 1 LASERAPPARAT VAROITUS! LAITTEEN KÄYTTÄMINEN MUULLA KUIN TÄSSÄ KÄYTTÖOHJEESSA MAINITULLA TAVALLA SAATTAA ALTISTAA KÄYTTÄJÄN TURVALLISUUSLUOKAN 1 YLITTÄVÄLLE NÄKYMÄTTÖMÄLLE LASERSÄTEILYLLE. VARNING OM APPARATEN ANVÄNDS PÅ ANNAT SÄTT ÄN I DENNA BRUKSANVISNING SPECIFICERATS, KAN ANVÄNDAREN UTSÄTTAS FÖR OSYNLIG LASERSTRÅLNING, SOM ÖVERSKRIDER GRÄNSEN FÖR LASERKLASS 1. Laserstrahl LASERSTRÅLING NÅR DEKSEL ÅPNES OG SIKKERHEDSLÅS BRYTES. LASER RADIATION WHEN OPEN AND INTERLOCKS DEFEATED. CAUTION INVISIBLE UNNGÅ EKSPONERING FOR STRÅLEN. ADVERSEL USYNLIG AVOID EXPOSURE TO BEAM. OSYNLIG LASERSTRÅLNING NÄR DENNA DEL ÄR ÖPPNAD OCH SPÄRRAR ÄR ...
ELISA method for the measurement of IgM isotype auto antibodies targeted to annexin V. Assay designed with highly purified human recombinant annexin V, obtained with non-denaturing methods, for auto antibody immunocapture. These are then revealed with a goat antibody specific for IgM, micro fragments, and labeled with peroxidase.
Mouse anti Human Annexin A3 antibody, clone 4F1 recognizes human annexin A3, also known as Annexin III, Inositol 1,2-cyclic phosphate 2-ph
Annexin A2 is a calcium-dependent, phospholipid-binding protein found on many cell types. It consists of a short hydrophobic tail (Ser2-Asn32), which dictates its function, and a core domain (Phe33-Asp339), which is involved in phospholipid binding. Annexin A2 has been implicated in a number of biochemical processes, including cell proliferation, foetal immune tolerance, ion-channel activation, cell-cell interactions and the bridging of membranes. Annexin A2 is reported to be a powerful activator of plasminogen and, therefore, is implicated in many normal and pathological processes such as haemostasis and metastasis. Myeloid cell lines are used, extensively, to study many aspects of cellular proliferation, differentiation and function. In the present study, we have used flow cytometry and real-time PCR to investigate the role of annexin A2 expression in the proliferation and differentiation of a number of myeloid cell lines. The results demonstrated that annexin A2 expression was affected when ...
Cortisone acetate is a steroid hormone that has both glucocoriticoid and mineral corticoid activities. Corticosteroids are used to provide relief for inflamed areas of the body. They lessen swelling, redness, itching, and allergic reactions. They are often used as part of the treatment for a number of different diseases, such as severe allergies or skin problems, asthma, or arthritis. Endogenous glucocorticoids and some synthetic corticoids have high affinity to the protein transcortin (also called CBG, corticosteroid-binding protein), whereas all of them bind albumin. Glucocorticoids also bind to the cytosolic glucocorticoid receptor.
Inom klinisk neuropatologisk (NP) diagnostik analyseras hjärnvävnad tillvaratagen vid obduktion, för att säkerställa vilken typ av demens en patient lidit av. Vid en NP undersökning undersöker man bland annat hur olika proteinuttryck ser ut i olika delar av hjärnan. Proteinuttryck i vävnad kan, med immunohistokemiska metoder (IHC), märkas in och göras synligt för mikroskopiundersökning. IHC resultat tillsammans med kliniskt ställd diagnos, makroskopiskt synlig degeneration och vävnadsförändringar påvisade med mikroskopi läggs samman vid NP-diagnostik. Syftet med denna studie var att undersöka hur tillförlitlig Tissue Microarray (TMA) metoden är för att analysera specifika proteinuttryck i hjärnvävnad från patienter med Frontotemporal degeneration (FTD) och Alzheimers sjukdom (AD) i förhållande till helsektionssnitt. Med TMA teknologin tas med en punktionsnål vävnadsstansar från ett flertal vävnadsklossar vilka komprimeras i en enda kloss. I studien extraherades ...
Efter 19 år i USA och 9 år som inredare i New York har jag nu öppnat min egen butik och inredningsfirma på Upplandsgatan 36 i Vasastan. Jag erbjuder nya varumärken från USA som aldrig tidigare sålts i Sverige. Bland annat doftljus i underbara förpackningar, läckra nya kuddar med tuffa tryck och fina accessoarer som passar till alla inredningar. Förutom härliga inredningsartiklar erbjuder jag även konsultation till alla som vill ändra om där hemma och skulle vilja ha lite extra hjälp. - AFTER 19 YEARS IN THE US. I AM NOW BACK IN MY HOMETOWN OF STOCKHOLM, SWEDEN AND HAVE VENTURED INTO A WHOLE NEW CHAPTER OF MY LIFE. I HAVE STARTED MY OWN BUSINESS AND HAVE OPENED A SHOP IN THE CENTER OF TOWN. I HOPE YOU ENJOY READING MY BLOG ...
Alclometasone is a synthetic corticosteroid for topical dermatologic use, possessing anti-inflammatory, antipruritic, and vasoconstrictive properties. The prodrug alclometasone dipropionate was originally marketed under the brand name Aclovate by GlaxoSmithKline as a topical cream and ointment. However, generic versions of the drug are available. Alclometasone induces the production of lipocortins, formally known as annexins, which inhibit phospholipase A2 - the enzyme responsible for the synthesis of arachidonic acid. Without the oxidation of arachidonic acid, eicosanoids, such as prostaglandins, thromboxanes, and leukotrienes, cant be produced. Alclometasone also inhibits the release of pro-inflammatory mediators from leukocytes (e.g., cytokines, histamine, leukotrienes, serotonin). Alclometasone cream and ointment are indicated for the relief of corticosteroid-responsive dermatoses, including: atopic dermatitis eczema psoriasis allergic dermatitis contact dermatitis actinic dermatitis ...
mouse Svs7 protein: a seminal plasma protein that binds phospholipids; isolated from murine sperm; consists of 76 amino acid residues with five disulfide bridges; RefSeq NM_020264
ATP- and membrane-binding protein that probably controls membrane reorganization/tubulation upon ATP hydrolysis. Plays a role in early endosomal transport.
ATP- and membrane-binding protein that probably controls membrane reorganization/tubulation upon ATP hydrolysis. Plays a role in early endosomal transport.
Like tissues, single cells are subjected to continual stresses and damage. As such, cells have a robust wound repair mechanism comprised of dynamic membrane resealing and cortical cytoskeletal remodeling. One group of proteins, the Rho family of small guanosine triphosphatases (GTPases), is critical for this actin and myosin cytoskeletal response in which they form distinct dynamic spatial and temporal patterns/arrays surrounding the wound. A key mechanistic question, then, is how these GTPase arrays are formed. Here, we show that in the Drosophila melanogaster cell wound repair model Rho GTPase arrays form in response to prepatterning by Rho guanine nucleotide exchange factors (RhoGEFs), a family of proteins involved in the activation of small GTPases. Furthermore, we show that Annexin B9, a member of a class of proteins associated with the membrane resealing, is involved in an early, Rho family-independent, actin stabilization that is integral to the formation of one RhoGEF array. Thus, ...
shark at sam.neosoft.com (John D. Valentich) wrote: , , We have recently started preparing polyclonal antibodies in rabbits against synthetic peptides , from annexins and phospholipase A2 activating protein (PLAP). Peptides were , conjugated to the carrier BSA. We are wondering about the problem of , antibodies generated against BSA reacting with serum albumin or albumin-like , epitopes in other cellular proteins. Questions: , , 1. Is it likely (or inevitable) that our antisera will contain high titer , antibodies against BSA? Could they be expected to cross react with other , non-albumin cellular proteins? They will almost certainly react with albumin. , , 2. If so, is the best (or only?) solution to this problem affinity purification of annexin or PLAP , antibodies from the antisera? Affinity purification should work. You could also adsorb the antisera with BSA and hope to remove many of the cross-reacting antibodies. Its probably too late now but you can use keyhole limpet hemocyanin (KLH) ...
Full Text - Vascular calcification is commonly seen in elderly people, though it can also appear in middle-aged subjects affected by premature vascular aging. The aim of this work is to test the involvement of microvesicles (MVs) produced by senescent endothelial cells (EC) and from plasma of elderly people in vascular calcification. The present work shows that MVs produced by senescent cultured ECs, plus those found in the plasma of elderly subjects, promote calcification in vascular smooth muscle cells. Only MVs from senescent ECs, and from elderly subjects plasma, induced calcification. This ability correlated with these types of MVs carriage of: a) increased quantities of annexins (which might act as nucleation sites for calcification), b) increased quantities of bone-morphogenic protein, and c) larger Ca contents. The MVs of senescent, cultured ECs, and those present in the plasma of elderly subjects, promote vascular calcification. The present results provide mechanistic insights into the
Synaptotagmins (Syts) are brain-specific Ca2+/phospholipid-binding proteins. In hippocampal synapses, Syt I is essential for fast Ca(2+)-dependent synaptic vesicle exocytosis but not for Ca(2+)-independent exocytosis. In vertebrates and invertebrates, Syt may therefore participate in Ca(2+)-dependent synaptic membrane fusion, either by serving as the Ca2+ sensor in the last step of fast Ca(2+)-triggered neurotransmitter release, or by collaborating with an additional Ca2+ sensor. While Syt I binds Ca2+ (refs 10, 11), its phospholipid binding is triggered at lower calcium concentrations (EC50 = 3-6 microM) than those required for exocytosis. Furthermore, Syts bind clathrin-AP2 with high affinity, indicating that they may play a general role in endocytosis rather than being confined to a specialized function in regulated exocytosis. Here we resolve this apparent contradiction by describing four Syts, three of which (Syt VI, VII and VIII) are widely expressed in non-neural tissues. All Syts tested ...
Synaptotagmins (Syts) are brain-specific Ca2+/phospholipid-binding proteins. In hippocampal synapses, Syt I is essential for fast Ca(2+)-dependent synaptic vesicle exocytosis but not for Ca(2+)-independent exocytosis. In vertebrates and invertebrates, Syt may therefore participate in Ca(2+)-dependent synaptic membrane fusion, either by serving as the Ca2+ sensor in the last step of fast Ca(2+)-triggered neurotransmitter release, or by collaborating with an additional Ca2+ sensor. While Syt I binds Ca2+ (refs 10, 11), its phospholipid binding is triggered at lower calcium concentrations (EC50 = 3-6 microM) than those required for exocytosis. Furthermore, Syts bind clathrin-AP2 with high affinity, indicating that they may play a general role in endocytosis rather than being confined to a specialized function in regulated exocytosis. Here we resolve this apparent contradiction by describing four Syts, three of which (Syt VI, VII and VIII) are widely expressed in non-neural tissues. All Syts tested ...
Systemic corticosteroid tfiamcinolone triamcinolone cream prescribing information mask the symptoms of infection and should not be used in cases of viral infection, informatiion infection, or bacterial infection that are not adequately controlled by antiinfective agents. Patients receiving immunosuppressives along with denosumab may be at a greater risk of developing an infection. Systemic glucocorticoids, such as triamcinolone, should be used with caution in patients with myasthenia gravis. At the cellular level, corticosteroids induce peptides called lipocortins. Drugs metabolized by CYP enzymes, including corticosteroids, may require dosage adjustments when administered concurrently with rifamycins ...
Digital Photography Review: All the latest digital camera reviews and digital imaging news. Lively discussion forums. Vast samples galleries and the largest database of digital camera specifications.
Digital Photography Review: All the latest digital camera reviews and digital imaging news. Lively discussion forums. Vast samples galleries and the largest database of digital camera specifications.
Pete Harris performs an old blues/ ragtime number - Simply Wild About My Good Cocaine at The Platform Tavern, Southampton. Filmed with a Sony Nex-5 with a Canon…
购买我们的重组人Annexin A3蛋白。Ab92929为全长蛋白,在大肠杆菌中生产并经过Western blot, SDS-PAGE实验验证。Abcam提供免费的实验方案,操作技巧及专业的支持。
Abstract: The control of lipid domain formation in biological membranes has received limited consideration. This mechanism is quantitatively investigated using Monte Carlo computer simulations of a simple model system. Monte Carlo simulations are performed on a simple model system composed of phosphatidylecholine (PC), phosphatidylserine (PS), and cholesterol (Chol). Domain formation induced by binding of the phospholipid binding proteins, annexin A5 (A5) and the C2 protein motif is investigated. Simulations for models containing PC/PS lipids indicate that the addition of A5 does not induce lipid domain formation while binding of C2 greatly induces lipid domain formation. The addition of Chol to PC/PS systems was found to induce lipid demixing in the absence and presence of A5 and further enhance the ability of C2 to form PS domains. Incorporation of a preferential protein-protein interaction to PC/PS and PC/PS/Chol systems was found to further increase lipid demixing for all compositions. Lipid ...
Annexin A1 is an intracellular calcium/phospholipid-binding protein that is involved in membrane organization and the regulation of the immune system. It has been attributed an anti-inflammatory role at various control levels, and recently we could show that annexin A1 externalization during secondary necrosis provides an important fail-safe mechanism counteracting inflammatory responses when the timely clearance of apoptotic cells has failed. As such, annexin A1 promotes the engulfment of dying cells and dampens the postphagocytic production of proinflammatory cytokines. In our current follow-up study, we report that exposure of annexin A1 during secondary necrosis coincided with proteolytic processing within its unique N-terminal domain by ADAM10. Most importantly, we demonstrate that the released peptide and culture supernatants of secondary necrotic, annexin A1-externalizing cells induced chemoattraction of monocytes, which was clearly reduced in annexin A1- or ADAM10-knockdown cells. Thus, ...
Animals. Wild-type mice from the 129T2/SvEmsJ background were bred and housed in a specific pathogen-free facility on a 12-hour light/12-hour dark cycle and fed ad libitum in accordance with the Northwestern Universitys Institutional Animal Care and Use Committee regulations. 129T2/SvEmsJ (129T2) mice were purchased from the Jackson Laboratory (stock 002065). mdx/hLTBP4 mice were generated as described previously (34, 60). Sgcg-null mice were generated as described in Hack et al. (46). Two- to 3-month-old males and females were used for all wild-type mouse experiments. Sgcg-null cohorts were age and sex matched with mice between 2 and 5 months old.. Plasmids. Plasmids encoding annexin A1, A2, and A6 with a carboxyl-terminal turboGFP tag were obtained from Origene. Subcloning of annexin A1, A2, and A6 to replace the GFP tag with tdTomato (Addgene) was performed by Mutagenix. Site-directed mutagenesis was performed by Mutagenix on annexin A1-GFP, A2-GFP, and A6-GFP to create the Ca2+-binding ...
Animals. Wild-type mice from the 129T2/SvEmsJ background were bred and housed in a specific pathogen-free facility on a 12-hour light/12-hour dark cycle and fed ad libitum in accordance with the Northwestern Universitys Institutional Animal Care and Use Committee regulations. 129T2/SvEmsJ (129T2) mice were purchased from the Jackson Laboratory (stock 002065). mdx/hLTBP4 mice were generated as described previously (34, 60). Sgcg-null mice were generated as described in Hack et al. (46). Two- to 3-month-old males and females were used for all wild-type mouse experiments. Sgcg-null cohorts were age and sex matched with mice between 2 and 5 months old.. Plasmids. Plasmids encoding annexin A1, A2, and A6 with a carboxyl-terminal turboGFP tag were obtained from Origene. Subcloning of annexin A1, A2, and A6 to replace the GFP tag with tdTomato (Addgene) was performed by Mutagenix. Site-directed mutagenesis was performed by Mutagenix on annexin A1-GFP, A2-GFP, and A6-GFP to create the Ca2+-binding ...
Several reports indicate that residues at the tip of the Ca2+-binding pockets, including the conserved basic residues, in both C2 domains of synaptotagmin are critical for Ca2+-dependent phospholipid interactions in vitro and Ca2+-triggered fusion in cultured cells (Chapman and Davis, 1998; Fernández-Chacón et al., 2001; Bai et al., 2002; Frazier et al., 2003; Sørensen et al., 2003; Wang et al., 2003; Rhee et al., 2005; Araç et al., 2006). Studying cultured cells provides valuable insight into possible protein functions, yet some of the properties of vesicle fusion in PC12 cells and chromaffin cells are quite different from those mediating fast transmitter release at a synapse. Even cultured neurons do not necessarily faithfully reproduce all aspects of synaptic behavior at an intact synapse. For example, at cultured hippocampal autapses, the amplitude of mEJPs is larger (Bekkers et al., 1990), the paired-pulse ratio is decreased (Sippy et al., 2003), and long-term potentiation cannot be ...
SCAN HISTORY OWL11_0 2 100 NSINGLE OWL17_1 2 110 NSINGLE OWL18_0 1 115 NSINGLE OWL19_1 1 125 NSINGLE OWL21_1 1 125 NSINGLE OWL26_0 1 125 NSINGLE SPTR37_9f 2 237 NSINGLE INITIAL MOTIF SETS ANNEXIN1 Length of motif = 23 Motif number = 1 Annexin motif I - 1 PCODE ST INT KTKGVDEVTIVNILTNRSNAQRQ LUHU36 46 46 KTKGVDEVTIINILTNRSNEQRQ ANX2_CHICK 46 46 TVKGVDEATIIDILTKRNNAQRQ ANX1_CAVCU 56 56 MVKGVDEATIIDILTKRNNAQRQ LUHU 55 55 KGIGTDEATIIDIVTHRSNAQRQ ANX6_MOUSE 376 376 KGMGTDEETILKILTSRNNAQRQ ANX5_CHICK 29 29 KGIGTNEQAIIDVLTKRSNTQRQ ANX8_HUMAN 35 35 KGFGTDEQEIIDVLVGRSNQQRQ DROANNX 29 29 RGIGTDEKMLISILTERSNAQRQ ANX3_HUMAN 32 32 KGLGTDEDAIINVLAYRSTAQRQ ANX4_BOVIN 27 27 KGFGTDEQAIVDVVANRSNDQRQ HUMSNEXIN 177 177 TAKGVDEATIIDIMTTRTNAQRP ANX1_COLLI 51 51 ANNEXIN2 Length of motif = 17 Motif number = 2 Annexin motif II - 1 PCODE ST INT LKSALSGHLETVILGLL LUHU36 86 17 LKSALSGHLEAVILGLL ANX2_CHICK 86 17 LKKALTGHLEEVVLALL ANX1_CAVCU 96 17 LKKALTGHLEEVVLALL LUHU 95 17 LKSEISGDLARLILGLM ANX6_MOUSE 416 17 ...
1HVD: Structural and electrophysiological analysis of annexin V mutants. Mutagenesis of human annexin V, an in vitro voltage-gated calcium channel, provides information about the structural features of the ion pathway, the voltage sensor and the ion selectivity filter
Taylor, SRJ, Gonzalez-Begne, M, Dewhurst, S, Chimini, G, Higgins, CF, Melvin, JE and Elliott, JI (2008) Sequential shrinkage and swelling underlie P2X(7)-stimulated lymphocyte phosphatidylserine exposure and death ...
Rabbit polyclonal Annexin VII antibody validated for WB, IHC and tested in Human. With 1 independent review. Immunogen corresponding to recombinant fragment
Annexin A2兔多克隆抗体(ab90643)可与人样本反应并经WB, IHC实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
TY - JOUR. T1 - Annexin-1 mediates TNF-α-stimulated matrix metalloproteinase secretion from rheumatoid arthritis synovial fibroblasts. AU - Tagoe, Clement E.. AU - Marjanovic, Nada. AU - Park, Jean Y.. AU - Chan, Edwin S.. AU - Abeles, Aryeh M.. AU - Attur, Mukundan. AU - Abramson, Steven B.. AU - Pillinger, Michael H.. PY - 2008/8/15. Y1 - 2008/8/15. N2 - Annexins are intracellular molecules implicated in the down-regulation of inflammation. Recently, annexin-1 has also been identified as a secreted molecule, suggesting it may have more complex effects on inflammation than previously appreciated. We studied the role of annexin-1 in mediating MMP-1 secretion from rheumatoid arthritis (RA) synovial fibroblasts (SF) stimulated with TNF-α. TNF-α induced a biphasic secretion of annexin-1 from RA SF. Early (≤60 min), cycloheximide-independent secretion from preformed intracellular pools was followed by late (24 h) cycloheximide-inhibitable secretion requiring new protein synthesis. Exogenous ...
The role of the calcium- and phospholipid-binding protein annexin I (ANXA1) in cell cycle regulation has been investigated in estrogen receptor (ER)-positive MCF-7 and ER-negative MDA-MB-231 breast tumor cell lines. In MCF-7 cells, ANXA1-targeting small interfering RNA (siRNA) reduced ANXA1 mRNA and protein levels and attenuated cell proliferation induced by FCS, estradiol, or epidermal growth factor. Well-characterized agonists for the known ANXA1 receptor, FPR2, including the ANXA1 N-terminal proteolytic product ANXA1(2-26), lipoxin A(4) (LXA(4)), and the synthetic peptide, Trp-Lys-Tyr-Met-Val-D-Met (WKYMVm), stimulated proliferation of MCF-7 and MDA-MB-231 cells that was attenuated by incubation with FPR2 antagonists WRW(4) (1 μM) or Boc2 (100 nM) or by siRNA against FPR2. FCS-induced mitogenic responses were attenuated by each of the FPR antagonists and by siRNA against FPR2 and, to a lesser extent, FPR1. LXA(4) increased phosphorylation of Akt, p70(S6K) but not ERK1/2. Increases in cyclin ...
Wells, D., Wells, K., Liu, K., Hannon, R., Croxtall, J.D., Damazo, A.S., Oliani, S.M., Getting, S.J., Parente, L., Paul-Clark, M., Yona, S., Gavins, F.N.E., Martin, J., Christian, H.C., Cover, P.O., John, C.D., Solito, E., Morris, J.F., Perretti, M., Buckingham, J.C. and Flower, R.J. 2004. The annexin 1-/- mouse: phenotypic studies. Annexins. 1 (2), pp. 109-120. Activation of melanocortin receptors induces cAMP-dependent hemeoxygenase-1 expression in murine macrophages ...
Annexin A6 (AnxA6) is a Ca2+-dependent membrane-binding protein involved in vesicular traffic. The likely participation of AnxA6 in the response of lymphocytes to Ca2+ signals has not been investigated yet. The present study focuses on intracellular relocation of AnxA6 in human Jurkat T lymphoblasts upon stimulation followed by transient increase of intracellular [Ca2+] and exocytosis of interleukin-2 (IL-2). Stimulation of the cells under different experimental conditions (by lowering pH and/or by rising extracellular [Ca2+] in the presence of ionomycin) induced time-dependent transients of intracellular [Ca2+] and concomitant changes in AnxA6 intracellular localization and in IL-2 secretion, with only minor effects on cell viability and apoptosis. In resting conditions (in the presence of EGTA or with no ionophore) AnxA6 was localized uniformly in the cytosol, whereas it translocated to vesicular structures beneath the plasma membrane within 5 min following stimulation of Jurkat T cells and ...

Annexins -  - kirja(9781461348412) | Adlibris kirjakauppaAnnexins - - kirja(9781461348412) | Adlibris kirjakauppa

Lähetetään 5‑9 arkipäivässä.. Osta kirja Annexins (ISBN 9781461348412) osoitteesta Adlibris.fi. Ilmainen toimitus Meillä on ... The Annexins is focused on a specific family of calcium and membrane-binding proteins, annexins, ubiquitously spread within ... provides an up-to-date overview of potential physiological roles of annexins. Growing evidence of participation of annexins in ... The Annexin V Binding Properties of Platelet-Derived Microparticles. Connor David. 68,50 € ...
more infohttps://www.adlibris.com/fi/kirja/annexins-9781461348412

The annexins of Dictyostelium.  - PubMed - NCBIThe annexins of Dictyostelium. - PubMed - NCBI

In cell fractionation studies, annexin C2 cofractionates with annexin C1 and is enriched in the 100,000 g pellet. Like annexin ... and the annexin C2 gene (nxnB) coding for a 56-kDa protein with 33% sequence identity to annexin C1. Annexin C2 is expressed at ... Annexin C2 is not observed in the nucleus. An annexin C1 mutant (SYN-) which shows a defect during multicellular development ... Analysis of the Dictyostelium genome revealed the presence of two annexin genes, the annexin C1 gene (nxnA) giving rise to two ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/16762449

annexin 6 [Arabidopsis thaliana] - Protein - NCBIannexin 6 [Arabidopsis thaliana] - Protein - NCBI

annexin 6 [Arabidopsis thaliana] annexin 6 [Arabidopsis thaliana]. gi,15238094,ref,NP_196584.1, ... Expression profiling of the Arabidopsis annexin gene family during germination, de-etiolation and abiotic stress. [Plant ... Expression profiling of the Arabidopsis annexin gene family during germination, de-etiolation and abiotic stress.. Cantero A, ... Encodes a calcium-binding protein annexin (AnnAt6). Also Known As: AT5G10220, ANNAT6, ANNE... ...
more infohttps://www.ncbi.nlm.nih.gov/protein/NP_196584

Addgene: mEmerald-Annexin-12Addgene: mEmerald-Annexin-12

Michael Davidsons lab contains the insert Annexin. This plasmid is available through Addgene. ... mEmerald-Annexin-12 was a gift from Michael Davidson (Addgene plasmid # 53991 ; http://n2t.net/addgene:53991 ; RRID:Addgene_ ...
more infohttp://www.addgene.org/53991/

Evrogen pmKate2-annexinEvrogen pmKate2-annexin

mKate2-annexin fusion: 603-2313. mKate2: 603-1314. Start codon (ATG): 603-605. Last amino acid in mKate2: 1312-1314. Annexin A4 ... pmKate2-annexin. FP321. 20 μg. € 400 / 200*. *50% discount on the second and subsequent vectors encoding same fluorescent ... pmKate2-annexin vector. cat.# FP321. The vector sequence has been compiled using the information from sequence databases, ...
more infohttp://evrogen.com/products/vectors/pmKate2-annexin/pmKate2-annexin_Locations.shtml

APC Annexin VAPC Annexin V

Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in ... Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in ... Annexin V Binding Buffer (Cat. No. 422201) is recommended for use with Annexin V staining. Annexin V binding alone cannot ... Why do I need to use Annexin V Binding Buffer? Annexin V binding requires the presence of calcium in the solution. So, we ...
more infohttps://www.biolegend.com/de-de/products/apc-annexin-v-8144

APC Annexin VAPC Annexin V

Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in ... Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in ... Annexin V Binding Buffer (Cat. No. 422201) is recommended for use with Annexin V staining. Annexin V binding alone cannot ... Why do I need to use Annexin V Binding Buffer? Annexin V binding requires the presence of calcium in the solution. So, we ...
more infohttps://www.biolegend.com/en-us/products/apc-annexin-v-8144

Annexin - Heterodera glycines (Soybean cyst nematode worm)Annexin - Heterodera glycines (Soybean cyst nematode worm)

IPR001464 Annexin. IPR018502 Annexin_repeat. IPR018252 Annexin_repeat_CS. IPR037104 Annexin_sf. ... IPR001464 Annexin. IPR018502 Annexin_repeat. IPR018252 Annexin_repeat_CS. IPR037104 Annexin_sf. ... A pair of annexin repeats may form one binding site for calcium and phospholipid.UniRule annotation. Automatic assertion ... Belongs to the annexin family.UniRule annotation. Automatic assertion according to rulesi ...
more infohttps://www.uniprot.org/uniprot/Q8ITJ0

ANXA1 (annexin A1)ANXA1 (annexin A1)

... annexin A1), Authors: Flavia Cristina Rodrigues-Lisoni, Tiago Henrique, Eloiza Helena Tajara. Published in: Atlas Genet ... Similarly to other annexins, annexin A1 is characterized by a C-terminal homologous domain with 4 to 8 repeats of 70-75 ... The comparison of conserved and variable residues between human annexin 1 protein and annexin protein from different ... Loss of annexin A1 expression in breast cancer progression.. Cao Y, Li Y, Edelweiss M, Arun B, Rosen D, Resetkova E, Wu Y, Liu ...
more infohttp://atlasgeneticsoncology.org/Genes/GC_ANXA1.html

ANXA1 (annexin A1)ANXA1 (annexin A1)

... annexin A1), Authors: Flavia Cristina Rodrigues-Lisoni, Tiago Henrique, Eloiza Helena Tajara. Published in: Atlas Genet ... Similarly to other annexins, annexin A1 is characterized by a C-terminal homologous domain with 4 to 8 repeats of 70-75 ... The comparison of conserved and variable residues between human annexin 1 protein and annexin protein from different ... Loss of annexin A1 expression in breast cancer progression.. Cao Y, Li Y, Edelweiss M, Arun B, Rosen D, Resetkova E, Wu Y, Liu ...
more infohttp://atlasgeneticsoncology.org/Genes/ANXA1ID653ch9q21.html

Anti-Annexin A1 antibody (ab97485) | AbcamAnti-Annexin A1 antibody (ab97485) | Abcam

Rabbit polyclonal Annexin A1 antibody validated for WB, IHC, ICC/IF and tested in Human. Immunogen corresponding to recombinant ... Anti-Annexin A1 antibody (ab97485) at 1/1000 dilution + H1299 whole cell lysate at 30 µg. Predicted band size : 39 kDa. ... A pair of annexin repeats may form one binding site for calcium and phospholipid. ... Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1-198 of Human Annexin A1 ( ...
more infohttp://www.abcam.com/annexin-a1-antibody-ab97485.html

Anti-Annexin VII antibody (ab97323) | AbcamAnti-Annexin VII antibody (ab97323) | Abcam

Rabbit polyclonal Annexin VII antibody validated for WB, IHC and tested in Human. With 1 independent review. Immunogen ... All lanes : Anti-Annexin VII antibody (ab97323) at 1/2000 dilution. Lane 1 : 293T whole cell lysate. Lane 2 : A431 whole cell ... A pair of annexin repeats may form one binding site for calcium and phospholipid. ... Recombinant protein fragment corresponding to a region within amino acids 122 and 429 of Human Annexin VII (NP_004025) ...
more infohttp://www.abcam.com/annexin-vii-antibody-ab97323.html

Annexin VAnnexin V

BioVision offers high quality annexin V products for fast detection of apoptosis in living cells. Annexin V conjugates are ... Complete kits of 25, 100, 400 assays sizes are available. Bulk annexin V reagents of 1000 assays size are also available, ... Annexin V Apoptosis Assay is so simple, takes only 10 minutes. Results can be analyzed by flow cytometry or fluorescence ...
more infohttps://www.biovision.com/products/apoptosis-related-products/annexin-v.html

ANXA1 - Annexin A1 - Rodentia sp. - ANXA1 gene & proteinANXA1 - Annexin A1 - Rodentia sp. - ANXA1 gene & protein

IPR001464. Annexin. IPR018502. Annexin_repeat. IPR018252. Annexin_repeat_CS. IPR037104. Annexin_sf. IPR002388. ANX1. ... IPR001464. Annexin. IPR018502. Annexin_repeat. IPR018252. Annexin_repeat_CS. IPR037104. Annexin_sf. IPR002388. ANX1. ... Annexin 1Add BLAST. 61. ,p>This subsection of the Family and Domains section indicates the positions and types of repeated ... Annexin 2Add BLAST. 61. ,p>This subsection of the Family and Domains section indicates the positions and types of repeated ...
more infohttp://www.uniprot.org/uniprot/P24551

WikiGenes - ANXA3 - annexin A3WikiGenes - ANXA3 - annexin A3

The crystal structure of annexin A8 is similar to that of annexin A3. Réty, S., Sopková-de Oliveira Santos, J., Dreyfuss, L., ... The high-resolution crystal structure of human annexin III shows subtle differences with annexin V. Favier-Perron, B., Lewit- ... We observed a distinct translocation of annexin V to DNP-HSA containing endosomes, and annexin III to vesicles with C. ... Both guinea pig kidney annexin III and human placental annexin III pellet with the addition of calcium and centrifugation, ...
more infohttps://www.wikigenes.org/e/gene/e/306.html

Annexin 2 Receptor RNAi: Novus BiologicalsAnnexin 2 Receptor RNAi: Novus Biologicals

Browse our Annexin 2 Receptor RNAi catalog backed by our Guarantee+. ... Alternate Names for Annexin 2 Receptor RNAi. Annexin 2 Receptor RNAi, ANXA2R RNAi, Annexin II receptor RNAi, annexin-2 receptor ... Annexin 2 Receptor RNAi. We offer Annexin 2 Receptor RNAi for use in common research applications. Each Annexin 2 Receptor RNAi ... Our Annexin 2 Receptor RNAi can be used in a variety of model species: Human. Use the list below to choose the Annexin 2 ...
more infohttps://www.novusbio.com/rnai/annexin-2-receptor

Annexin V Antibody Pairs: Novus BiologicalsAnnexin V Antibody Pairs: Novus Biologicals

Browse our Annexin V Antibody Pair catalog backed by our Guarantee+. ... Annexin V Antibody Pairs available through Novus Biologicals. ... Annexin V Antibody Pairs. We offer Annexin V Antibody Pairs for ... Alternate Names for Annexin V Antibody Pairs. Annexin V antibody pair, ANXA5 antibody pair, Anchorin CII antibody pair, annexin ... Our Annexin V Antibody Pairs can be used in a variety of model species: Human. Use the list below to choose the Annexin V ...
more infohttps://www.novusbio.com/antibody-pairs/annexin-v

Trypsin and AnnexinTrypsin and Annexin

We are ,seeing what we think is an exagerated Annexin response. Does anyone out ,there have any feedback on how trypsin affects ... Julie At 02:11 PM 8/18/98, you wrote: , ,I have a client who is staining with Annexin-FITC. They are looking at ,cell lines and ... Trypsin and Annexin Julie Auger jauger at flowcity.bsd.uchicago.edu Thu Aug 20 12:23:43 EST 1998 *Previous message: Call for ... In our hands, the trypsin actually reduced the Annexin staining presumably by stripping off all the phosphotidyl-serine on the ...
more infohttps://lists.purdue.edu/pipermail/cytometry/1998-August/010871.html

Annexin V-FITCAnnexin V-FITC

The Annexin V-FITC kit includes Annexin V-FITC for detecting apoptosis and also propidium iodide (PI) for detecting necrosis. ... The Annexin VFITC Plus kit includes cytox green dye (instead of PI) which stains necrotic cells with a higher level of green ... Annexin V-FITC conjugate is specially engineered to produce enhanced fluorescence signal and photo stability. ... Annexin V-FITC. img.floatRight {float: right;margin: 6px;text-align: justify;}.paraone {height:auto;}. The one-step staining ...
more infohttps://www.biovision.com/products/apoptosis-related-products/annexin-v/annexin-v-fitc.html

Anxa3 (annexin A3) - Rat Genome DatabaseAnxa3 (annexin A3) - Rat Genome Database

ANXA3 (annexin A3). NCBI. Ortholog. Mus musculus (house mouse):. Anxa3 (annexin A3). Transitive Ortholog Pipeline. Transitive ... Anxa3 (annexin A3). Transitive Ortholog Pipeline. Transitive Ortholog Pipeline. Sus scrofa (pig):. ANXA3 (annexin A3). ... ANXA3 (annexin A3). Transitive Ortholog Pipeline. Transitive Ortholog Pipeline. Canis lupus familiaris (dog):. ANXA3 (annexin ... Homo sapiens (human) : ANXA3 (annexin A3) HGNC Alliance Mus musculus (house mouse) : Anxa3 (annexin A3) MGI Alliance Rattus ...
more infohttps://rgd.mcw.edu/rgdweb/report/gene/main.html?id=8764586

Neonatal Hyperoxic Exposure Persistently Alters Lung Secretoglobins and Annexin A1Neonatal Hyperoxic Exposure Persistently Alters Lung Secretoglobins and Annexin A1

... Thomas M. Raffay,1 Morgan L. Locy,2 Cynthia ... Expression of lung SCGB and annexin A1 (ANXA1) is persistently altered in CCSP knockout mice suggesting that CCSP indirectly ...
more infohttps://www.hindawi.com/journals/bmri/2013/408485/abs/

annexin A5 - Semantic Scholarannexin A5 - Semantic Scholar

A protein of the annexin family isolated from human PLACENTA and other tissues. It inhibits cytosolic PHOSPHOLIPASE A2, and ... annexin A5. Known as: Calphobindin I, Annexin V, Lipocortin-V (More). A protein of the annexin family isolated from human ... Annexin A5, human. diannexin, human. technetium 99m tricarbonyl His-annexin A5. technetium Tc 99m HYNIC annexin V ... Annexin A5 polymorphism (-1C--,T) and the presence of anti-annexin A5 antibodies in the antiphospholipid syndrome. ...
more infohttps://www.semanticscholar.org/topic/annexin-A5/115736

Annexin - WikipediaAnnexin - Wikipedia

However some annexins (Annexin A1, Annexin A2, and Annexin A5) have also been found outside the cellular environment, for ... Annexin, type I InterPro: IPR002388 Annexin, type II InterPro: IPR002389 Annexin, type III InterPro: IPR002390 Annexin, type IV ... In addition to annexin A-II, annexin A-XI has also been shown to organize cell membrane properties. Annexin A-XI is believed to ... The 310 amino acid annexin core has four annexin repeats, each composed of 5 alpha-helices. The exception is annexin A-VI that ...
more infohttps://en.wikipedia.org/wiki/Annexin

Product Overview anti-Annexin VII AntibodiesProduct Overview anti-Annexin VII Antibodies

Selected quality suppliers for anti-Annexin VII antibodies. ... Order monoclonal and polyclonal Annexin VII antibodies for many ... Protein level used designations for anti-Annexin A7 (ANXA7) Antibodies annexin VII , annexin-7 , annexin A7 , synexin , annexin ... Annexin VII (ANXA7) Antigen Profile Protein Summary Annexin VII is a member of the annexin family of calcium-dependent ... anti-Annexin A7 (ANXA7) Antibodies. Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding ...
more infohttps://www.antibodies-online.com/abstract/Annexin+A7+

Product Overview anti-Annexin A13 AntibodiesProduct Overview anti-Annexin A13 Antibodies

Selected quality suppliers for anti-Annexin A13 antibodies. ... Order monoclonal and polyclonal Annexin A13 antibodies for many ... ANX XIII , annexin 13 , annexin A13 , annexin XIII , annexin, intestine-specific , annexin-13 , intestine-specific annexin , ... anti-Annexin A13 (ANXA13) Antibodies. ANXA13 encodes a member of the annexin family. Additionally we are shipping Annexin A13 ... Annexin A13 (ANXA13) Antigen Profile Protein Summary This gene encodes a member of the annexin family. Members of this calcium- ...
more infohttp://www.antibodies-online.com/abstract/Annexin+A13+
  • Annexins are important in various cellular and physiological processes such as providing a membrane scaffold, which is relevant to changes in the cell's shape. (wikipedia.org)
  • Our Annexin 2 Receptor RNAi can be used in a variety of model species: Human. (novusbio.com)
  • in vascular smooth muscle cells(VSMS) decreases the secretion of annexin II , increased production of reactive oxygen species (ROS), decreased angiogenesis, activated Wnt / beta-catenin signaling pathway via translocation of betacore catenin in VSMC. (thefreedictionary.com)
  • Growing evidence of participation of annexins in human diseases, called annexinopathies, related to disturbances in signal transduction, vesicular traffic, ion homeostasis and energy metabolism within the cell. (adlibris.com)
  • The comparison of conserved and variable residues between human annexin 1 protein and annexin protein from different vertebrates shows amino acid identities of 100% (Pan troglodytes), 91% (Canis familiars), 87% (Mus musculus), 89% (Rattus norvegicus) and 77% (Gallus gallus) (Rodrigues-Lisoni et al. (atlasgeneticsoncology.org)
  • Recombinant human annexin is the core targeting component of Theseus Imaging Corporation's Apomate(TM) imaging agent now under development for the noninvasive assessment of early response to anti-cancer treatment and for evaluation of myocardial damage in acute myocardial infarction and cardiac transplant rejection. (thefreedictionary.com)
  • Annexin is a naturally occurring human protein, produced by recombinant techniques, which is the key component of the Apomate(TM) kit. (thefreedictionary.com)
  • Annexin II overexpressien is correlated with poor prognosis in human gastric carcinoma. (thefreedictionary.com)
  • Recombinant protein encompassing a sequence within the center region of human Annexin IV. (genetex.com)
  • it is likely that nuclear annexin A1 is involved in DNA replication, especially DNA damage induced gene mutation, since DNA damage induced mutagenesis plays an important role in tumorigenesis. (intechopen.com)
  • In some annexins it can become phosphorylated and can cause affinity changes for calcium in the core region or alter cytoplasmic protein interaction. (wikipedia.org)
  • We offer Annexin 2 Receptor RNAi for use in common research applications. (novusbio.com)
  • Each Annexin 2 Receptor RNAi is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. (novusbio.com)
  • Choose from our Annexin 2 Receptor RNAi. (novusbio.com)
  • Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. (biolegend.com)
  • Annexin V binding alone cannot differentiate between apoptotic cells and necrotic. (biolegend.com)
  • The Annexin VFITC Plus kit includes cytox green dye (instead of PI) which stains necrotic cells with a higher level of green fluorescence than FITC does. (biovision.com)
  • Also, annexins have been shown to be involved in trafficking and organization of vesicles, exocytosis, endocytosis and also calcium ion channel formation. (wikipedia.org)
  • Annexin A5 uptake in ischemic myocardium: demonstration of reversible phosphatidylserine externalization and feasibility of radionuclide imaging. (semanticscholar.org)