Annexins
Family of calcium- and phospholipid-binding proteins which are structurally related and exhibit immunological cross-reactivity. Each member contains four homologous 70-kDa repeats. The annexins are differentially distributed in vertebrate tissues (and lower eukaryotes) and appear to be involved in MEMBRANE FUSION and SIGNAL TRANSDUCTION.
Annexin A4
Annexin A2
A member of the annexin family that is a substrate for a tyrosine kinase, ONCOGENE PROTEIN PP60(V-SRC). Annexin A2 occurs as a 36-KDa monomer and in a 90-KDa complex containing two subunits of annexin A2 and two subunits of S100 FAMILY PROTEIN P11. The monomeric form of annexin A2 was formerly referred to as calpactin I heavy chain.
Annexin A3
Annexin A5
Annexin A7
Calcium-Binding Proteins
Phosphatidylserines
Cypriniformes
Methylthiouracil
Calcium
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Tetraphenylborate
Killifishes
Phospholipids
Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.
Characterization of the calcyclin (S100A6) binding site of annexin XI-A by site-directed mutagenesis. (1/440)
Residues in annexin XI-A essential for binding of calcyclin (S100A6) were examined by site-directed mutagenesis. GST fusion proteins with the calcyclin binding site of annexin XI-A, GST-AXI 34-62 and GST-AXI 49-77 bound to calcyclin-Sepharose Ca2+-dependently. The mutants GST-AXI L52E, M55E, A56E and M59E lost the binding ability, whereas GST-AXI A57E retained the ability. These results demonstrate that the hydrophobic residues L52, M55, A56 and M59 on one side surface of the alpha-helix are critical for the binding. Assays with GST fusion proteins and synthesized peptides corresponding to the calcyclin binding site indicated that other regions around the calcyclin binding site are important to stabilize the conformation. (+info)GTPase activity and biochemical characterization of a recombinant cotton fiber annexin. (2/440)
A cDNA encoding annexin was isolated from a cotton (Gossypium hirsutum) fiber cDNA library. The cDNA was expressed in Escherichia coli, and the resultant recombinant protein was purified. We then investigated some biochemical properties of the recombinant annexin based on the current understanding of plant annexins. An "add-back experiment" was performed to study the effect of the recombinant annexin on beta-glucan synthase activity, but no effect was found. However, it was found that the recombinant annexin could display ATPase/GTPase activities. The recombinant annexin showed much higher GTPase than ATPase activity. Mg2+ was essential for these activities, whereas a high concentration of Ca2+ was inhibitory. A photolabeling assay showed that this annexin could bind GTP more specifically than ATP. The GTP-binding site on the annexin was mapped into the carboxyl-terminal fourth repeat of annexin from the photolabeling experiment using domain-deletion mutants of this annexin. Northern-blot analysis showed that the annexin gene was highly expressed in the elongation stages of cotton fiber differentiation, suggesting a role of this annexin in cell elongation. (+info)Transcription, biochemistry and localization of nematode annexins. (3/440)
The transcription of three annexin genes in the nematode, Caenorhabditis elegans, was detected by reverse transcriptase/polymerase chain reaction amplification of messenger RNAs. The highest level of expression was from the nex-1 gene, with lower levels detected for the nex-2 and nex-3 genes. The expression of nex-1 was reduced in the Dauer larval stage relative to the other annexins, correlating with the absence of the spermathecal valves, a major site of nex-1 protein localization. Recombinant nex-1 protein was expressed in yeast, isolated by calcium-dependent binding to acidic phospholipids, and its membrane binding and aggregating activities characterized using bovine chromaffin granules as a representative intracellular substrate. Binding to granule membranes was promoted by calcium with half-maximal binding seen at 630 microM calcium. Chromaffin granule aggregation was similarly promoted by the nex-1 protein at 630 microM calcium. This low sensitivity to calcium suggests the annexin can only be activated in vivo near the plasma membrane or other sources of calcium. Sequences including the nex-1 promoter were fused to the gene for green fluorescent protein and this construct was introduced into nematodes by microinjection. Examination of transgenic offspring revealed specific nex-1 promoter activity in the pharynx, the hypodermal cells, the vulva, and the spermathecal valve, locations in which the annexin may function in collagen secretion/deposition and membrane-membrane interactions. A sensitive anti-nex-1 antibody labelled with rhodamine was injected into body cavities of the nematode but did not detect extracellular nex-1 protein. Therefore, this annexin is apparently cytosolic and may function on the cytoplasmic side of the plasma membrane of the spermathecal valve to chaperon the folding of this membrane during the opening and closing of the valve. (+info)Annexin VII and annexin XI are tyrosine phosphorylated in peroxovanadate-treated dogs and in platelet-derived growth factor-treated rat vascular smooth muscle cells. (4/440)
The intraperitoneal administration of peroxovanadate results in the rapid accumulation of many tyrosine-phosphorylated proteins in the liver and kidney of treated animals. The availability of large pools of tyrosine-phosphorylated proteins derived from normal tissues facilitates the purification and identification of previously unknown targets for cellular tyrosine kinases. Using this procedure, we have thus far identified four proteins in the liver and kidney of peroxovanadate-treated dogs. Two of these, annexin VII and annexin XI, were novel and had not been previously reported to be substrates of tyrosine kinases while the remaining two, ezrin and clathrin, have been reported to be tyrosine phosphorylated in some cell culture systems. In the present study, isolated proteins were identified both by sequence analysis and immunological methods. Annexin VII and annexin XI are present in cultured rat vascular smooth muscle cells and both were tyrosine phosphorylated in response to a physiological ligand, platelet-derived growth factor-BB (PDGF-BB). Furthermore, the extent of tyrosine phosphorylation in response to PDGF-BB was augmented by the co-addition of peroxovanadate to cell cultures. In vitro phosphorylation assays showed that PDGF receptor, calcium-dependent tyrosine kinase (CADTK/Pyk-2), Src kinase, and epidermal growth factor receptor all were able to phosphorylate purified annexin VII and XI on tyrosine residues. These findings confirm the usefulness of phosphatase inhibition by peroxovanadate as a tool for identifying previously unknown physiological targets for cellular protein tyrosine kinases. (+info)Immunological development and cardiovascular function are normal in annexin VI null mutant mice. (5/440)
Annexins are calcium-binding proteins of unknown function but which are implicated in important cellular processes, including anticoagulation, ion flux regulation, calcium homeostasis, and endocytosis. To gain insight into the function of annexin VI, we performed targeted disruption of its gene in mice. Matings between heterozygous mice produced offspring with a normal Mendelian pattern of inheritance, indicating that the loss of annexin VI did not interfere with viability in utero. Mice lacking annexin VI reached sexual maturity at the same age as their normal littermates, and both males and females were fertile. Because of interest in the role of annexin VI in cardiovascular function, we examined heart rate and blood pressure in knockout and wild-type mice and found these to be identical in the two groups. Similarly, the cardiovascular responses of both sets of mice to septic shock were indistinguishable. We also examined components of the immune system and found no differences in thymic, splenic, or bone marrow lymphocyte levels between knockout and wild-type mice. This is the first study of annexin knockout mice, and the lack of a clear phenotype has broad implications for current views of annexin function. (+info)Annexins VII and XI are present in a human macrophage-like cell line. Differential translocation on FcR-mediated phagocytosis. (6/440)
We have studied the divalent cation-dependent association of proteins to subcellular fractions of human macrophage-like cells before and after FcR-mediated phagocytosis. Among these proteins we have identified annexins VII and XI for the first time in these cells, along with annexins I, III, and VI. Although all of these annexins are present in the cytosolic fraction, the extent of their association to membrane and phagosome fractions from resting and stimulated cells is variable. Annexin VII translocates from cytosolic to membrane fractions after phagocytic stimulation, along with annexin I, III, and VI. Annexins VII and XI are found associated with purified phagosomes along with I, III, and VI, and this association is greater after a 24-h chase period. Our results show differences in the intracellular distribution of different annexins in macrophage-like cells on phagocytosis. Annexins VII, VI, III, and I respond to particle ingestion by translocating to phagosomes and other cell membrane structures, whereas annexin XI translocates predominantly to phagosomes, suggesting dissimilarities in their function. (+info)Cellubrevin is present in the basolateral endocytic compartment of hepatocytes and follows the transcytotic pathway after IgA internalization. (7/440)
The endocytic compartment of polarized cells is organized in basolateral and apical endosomes plus those endocytic structures specialized in recycling and transcytosis, which are still poorly characterized. The complexity of the various populations of endosomes has been demonstrated by the exquisite repertoire of endogenous proteins. In this study we examined the distribution of cellubrevin in the endocytic compartment of hepatocytes, since its intracellular location and function in polarized cells are largely unknown. Highly purified rat liver endosomes were isolated from estradiol-treated rats, and the early/sorting endosomal fraction was further subfractionated in a multistep sucrose density gradient, and studied. Analysis of dissected endosomal fractions showed that cellubrevin was located in early/sorting endosomes, with Rab4, annexins II and VI, and transferrin receptor, but in a specific subpopulation of these early endosomes with the same density range as pIgA and Raf-1. Interestingly, only in those isolated endosomal fractions, endosomes enriched in transcytotic structures (of livers loaded with IgA), the polymeric immunoglobulin receptor specifically co-immunoprecipitated with cellubrevin. In addition, confocal and immuno-electron microscopy identification of cellubrevin in tubular structures underneath the sinusoidal plasma membrane together with the re-organization of cellubrevin, in the endocytic compartment, after the IgA loading, strongly suggest the involvement of cellubrevin in the transcytosis of pIgA. (+info)Annexin 24 from Capsicum annuum. X-ray structure and biochemical characterization. (8/440)
This work provides the first three-dimensional structure of a member of the plant annexin family and correlates these findings with biochemical properties of this protein. Annexin 24(Ca32) from Capsicum annuum was purified as a native protein from bell pepper and was also prepared by recombinant techniques. To overcome the problem of precipitation of the recombinant wild-type protein in crystallization trials, two mutants were designed. Whereas an N-terminal truncation mutant turned out to be an unstable protein, the N-terminal His-tagged annexin 24(Ca32) was crystallized, and the three-dimensional structure was determined by x-ray diffraction at 2. 8 A resolution. The structure refined to an R-factor of 0.216 adopts the typical annexin fold; the detailed structure, however, is different from non-plant annexins, especially in domains I and III and in the membrane binding loops on the convex side. Within the unit cell there are two molecules per asymmetric unit, which differ in conformation of the IAB-loop. Both conformers show Trp-35 on the surface. The loop-out conformation is stabilized by tight interactions of this tryptophan with residue side chains of a symmetry-related molecule and enforced by a bound sulfate. Characterization of this plant annexin using biophysical methods revealed calcium-dependent binding to phospholipid vesicles with preference for phosphatidylcholine over phosphatidylserine and magnesium-dependent phosphodiesterase activity in vitro as shown with adenosine triphosphate as the substrate. A comparative unfolding study of recombinant annexin 24(Ca32) wild type and of the His-tag fusion protein indicates higher stability of the latter. The effect of this N-terminal modification is also visible from CD spectra. Both proteins were subjected to a FURA-2-based calcium influx assay, which gave high influx rates for the wild-type but greatly reduced influx rates for the fusion protein. We therefore conclude that the N-terminal domain is indeed a major regulatory element modulating different annexin properties by allosteric mechanisms. (+info)
Annexin
However some annexins (Annexin A1, Annexin A2, and Annexin A5) can be secreted from the cytoplasm to outside cellular ... Annexin, type I InterPro: IPR002388 Annexin, type II InterPro: IPR002389 Annexin, type III InterPro: IPR002390 Annexin, type IV ... In addition to annexin A-II, annexin A-XI has also been shown to organize cell membrane properties. Annexin A-XI is believed to ... The 310 amino acid annexin core has four annexin repeats, each composed of 5 alpha-helices. The exception is annexin A-VI that ...
Annexin Pharmaceuticals
The concept for the therapy is based on the anti-inflammatory properties of Annexin A5, a body own protective protein that acts ... Annexin Pharmaceuticals is a Swedish privately held biotech company developing new therapeutic approaches for inflammatory ... The company is currently focusing on treatment of peripheral artery disease (PAD). Martz, Lauren (May 26, 2014). "Annexin: ...
Annexin A13
... is a protein that in humans is encoded by the ANXA13 gene. This gene encodes a member of the annexin family. ... "Comparative genetics and evolution of annexin A13 as the founder gene of vertebrate annexins". Molecular Biology and Evolution ... "Entrez Gene: ANXA13 annexin A13". Human ANXA13 genome location and ANXA13 gene details page in the UCSC Genome Browser. Wice BM ... "Apical membrane targeting of Nedd4 is mediated by an association of its C2 domain with annexin XIIIb". The Journal of Cell ...
Annexin A3
"The high-resolution crystal structure of human annexin III shows subtle differences with annexin V.". Biochemistry. 35 (6): ... Annexin A3 is a protein that in humans is encoded by the ANXA3 gene. It is abnormally expressed in fetuses of both IVF and ICSI ... "Entrez Gene: ANXA3 annexin A3". Zhang Y, Zhang YL, Feng C, et al. (September 2008). "Comparative proteomic analysis of human ... 2005). "Annexin A3 is a potential angiogenic mediator". Biochem. Biophys. Res. Commun. 337 (4): 1283-7. doi:10.1016/j.bbrc. ...
Annexin A2
... also known as annexin II is a protein that in humans is encoded by the ANXA2 gene. Annexin 2 is involved in diverse ... Kwon M, MacLeod TJ, Zhang Y, Waisman DM (January 2005). "S100A10, annexin A2, and annexin a2 heterotetramer as candidate ... Annexin II is a pleiotropic protein meaning that its function is dependent on place and time in the body. The ANXA2 gene, ... "Entrez Gene: ANXA2 annexin A2". Kling T, Ferrarese R, Ó hAilín D, Johansson P, Heiland DH, Dai F, et al. (October 2016). " ...
Annexin A4
... is a protein that in humans is encoded by the ANXA4 gene. Annexin IV (ANX4) belongs to the annexin family of calcium ... "Entrez Gene: ANXA4 annexin A4". Human ANXA4 genome location and ANXA4 gene details page in the UCSC Genome Browser. Römisch J, ... Dreier R, Schmid KW, Gerke V, Riehemann K (Aug 1998). "Differential expression of annexins I, II and IV in human tissues: an ... Han EK, Tahir SK, Cherian SP, Collins N, Ng SC (Jul 2000). "Modulation of paclitaxel resistance by annexin IV in human cancer ...
Annexin A6
... is a protein that in humans is encoded by the ANXA6 gene. Annexin VI belongs to a family of calcium-dependent ... It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Exon 21 of annexin VI is ... The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of ... Garbuglia M, Verzini M, Donato R (Sep 1998). "Annexin VI binds S100A1 and S100B and blocks the ability of S100A1 and S100B to ...
Annexin A1
... , also known as lipocortin I, is a protein that is encoded by the ANXA1 gene in humans. Annexin A1 belongs to the ... Annexin A1 has also been shown to be associated with treatment resistance. ARID1A loss activates annexin A1 expression, which ... Annexin A1 has been of interest for use as a potential anticancer drug. Upon induction by modified NSAIDS and other potent anti ... Annexin A1 protects against DNA damage induced by heat in breast cancer cells, adding to the evidence that it has tumor ...
Annexin A9
... is a protein that in humans is encoded by the ANXA9 gene. The annexins are a family of calcium-dependent ... Goebeler V, Ruhe D, Gerke V, Rescher U (Jul 2003). "Atypical properties displayed by annexin A9, a novel member of the annexin ... Members of the annexin family contain 4 internal repeat domains, each of which includes a type II calcium-binding site. The ... "Entrez Gene: ANXA9 annexin A9". Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of ...
Annexin A11
... is a protein that in humans is encoded by the ANXA11 gene. This gene encodes a member of the annexin family, a ... Farnaes L, Ditzel HJ (Aug 2003). "Dissecting the cellular functions of annexin XI using recombinant human annexin XI-specific ... "The penta-EF-hand domain of ALG-2 interacts with amino-terminal domains of both annexin VII and annexin XI in a Ca2+-dependent ... "The penta-EF-hand domain of ALG-2 interacts with amino-terminal domains of both annexin VII and annexin XI in a Ca2+-dependent ...
Annexin A7
... is a protein that in humans is encoded by the ANXA7 gene. Annexin VII is a member of the annexin family of calcium- ... "The penta-EF-hand domain of ALG-2 interacts with amino-terminal domains of both annexin VII and annexin XI in a Ca2+-dependent ... "The penta-EF-hand domain of ALG-2 interacts with amino-terminal domains of both annexin VII and annexin XI in a Ca2+-dependent ... The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in ...
Annexin A5
... (or annexin V) is a cellular protein in the annexin group. In flow cytometry, annexin V is commonly used to detect ... The annexin A5 affinity assay typically uses a conjugate of annexin V and a fluorescent or enzymatic label, biotin or other ... Annexin A5 showed upregulation in papillary thyroid carcinoma. Annexin A5 is used as a non-quantitative probe to detect cells ... Annexin A5 forms a shield around negatively charged phospholipid molecules. The formation of an annexin A5 shield blocks the ...
Annexin A5 affinity assay
This clustering of annexin A5 on the membrane greatly increases the intensity of annexin A5 when labeled with a fluorescent or ... Labeling of annexin A5 with fluorescent or radioactive molecules makes it possible to detect binding of labeled annexin A5 to ... After binding to the phospholipid surface, annexin A5 assembles into a trimeric cluster. This trimer consists of three annexin ... Internalization amplifies additionally the intensity of the annexin A5 stained cell. Annexin A5 has been used to successively ...
ANXA8L2
Annexin A8-like protein 2 is a protein that in humans is encoded by the ANXA8L2 gene. This gene encodes a member of the annexin ... "Entrez Gene: ANXA8 annexin A8". Human ANXA8 genome location and ANXA8 gene details page in the UCSC Genome Browser. Human ... 2006). "Annexin A8 is up-regulated during mouse mammary gland involution and predicts poor survival in breast cancer". Clin. ... Gerke V, Creutz CE, Moss SE (2005). "Annexins: linking Ca2+ signaling to membrane dynamics". Nat. Rev. Mol. Cell Biol. 6 (6): ...
Niche (protein structural motif)
Proteins with regulatory cations often employ niches for metal binding (e.g., thrombin, Na+; annexin, Ca2+; pyruvate ...
Johan Frostegård
"History - Annexin Pharmaceuticals". Annexin Pharmaceuticals. Retrieved 2018-09-02. "Nasdaq Stockholm Welcomes Annexin ... Medirista Biotechnologies AB and Annexin Pharmaceuticals AB, the latter being listed on Nasdaq First North since 2017. ...
Peripheral membrane protein
"Pfam entry: Annexin". Archived from the original on 2007-09-29. Retrieved 2007-01-25. "Pfam entry Synapsin N". Archived from ... The formation of ionic bridges is seen in the protein-lipid interaction between both protein C2 type domains and annexins.. Any ... They may also occur in BcL-2 like protein , in some amphiphilic antimicrobial peptides , and in certain annexins . These ... as in annexins or GLA domains. These peripheral proteins function as carriers of non-polar compounds between different types of ...
S100A10
Annexin II, which is attracted to negatively charged phospholipids, binds to p11 at the Ca2+ binding site. In addition, Annexin ... Kwon M, MacLeod TJ, Zhang Y, Waisman DM (Jan 2005). "S100A10, annexin A2, and annexin a2 heterotetramer as candidate ... Complexed with the annexin II, p11 binds receptor and channel proteins and guides them to the cell surface, resulting in ... P11 and annexin II form a heterotetrameric protein complex that imitates the structure and function of S-100 proteins activated ...
Gunilla Carlsson
Board of Directors Annexin Pharmaceuticals. High-Level Group of Personalities on Africa-Europe Relations Africa Europe ... Tundra Fonder, Member of the Board of Directors (since 2017) Annexin Pharmaceuticals, Member of the Board of Directors NGS ...
Mechanotransduction
Von Der Mark, K.; Mollenhauer, J. (1997). "Annexin V interactions with collagen". Cellular and Molecular Life Sciences. 53 (6 ... annexin V (a collagen type II receptor), and integrin receptors (of which there exist several types on chondrocytes). Using the ... and annexin V". Journal of Orthopaedic Research. 19 (6): 1122-30. doi:10.1016/S0736-0266(01)00051-1. PMID 11781014. Pulai, ...
Severe cutaneous adverse reactions
... interleukin 5 and annexin A1). DRESS syndrome: Key elements promoting tissue injury in the DRESS syndrome are: Th2 cells and ...
S100A11
... intermediate filaments also with annexin I and annexin II. S100A11 is able to control reorganization of actin and it is ... Seemann J, Weber K, Gerke V (August 1997). "Annexin I targets S100C to early endosomes". FEBS Letters. 413 (1): 185-90. doi: ... "Calcium-dependent binding of S100C to the N-terminal domain of annexin I". The Journal of Biological Chemistry. 271 (2): 719-25 ... the N-terminal part of annexin I". Structure. 8 (2): 175-84. doi:10.1016/s0969-2126(00)00093-9. PMID 10673436. Shankar J, ...
Sulfatide
... sulfatide binding to annexin V accelerates coagulation; third, sulfatide and P-selectin interactions expressed on platelets, ...
Mary Ann McLaughlin
Annexin II and bleeding in acute promyelocytic leukemia. N Engl J Med 1991; 340: 994-1004. Mclaughlin M, Fuster V. Three ...
Dysferlin
Lennon NJ, Kho A, Bacskai BJ, Perlmutter SL, Hyman BT, Brown RH (2003). "Dysferlin interacts with annexins A1 and A2 and ... including annexin and MG53. Exactly how dysferlin contributes to membrane resealing is not clear, but biochemical evidence ... "Enhanced Muscular Dystrophy from Loss of Dysferlin Is Accompanied by Impaired Annexin A6 Translocation after Sarcolemmal ...
Calmodulin
The other group, called annexins, bind calcium and phospholipids such as lipocortin. Many other proteins bind calcium, although ...
Glycerophosphoinositol inositolphosphodiesterase
Perron and co-workers confirmed on the basis of structural studies that annexin III did not possess an enzymatic activity. ... This enzyme 1-D-myo-inositol-1,2-cyclic-phosphate 2-inositolphosphohydrolase, was reported to be identical to annexin III. ... be inferred from structural studies of annexin III?". J. Biol. Chem. 272 (17): 11321-11326. doi:10.1074/jbc.272.17.11321. PMID ... "Dissociation of cyclic inositol phosphohydrolase activity from annexin III". J. Biol. Chem. 271 (14): 8295-8299. doi:10.1074/ ...
Jean Gruenberg
Morel, Etienne; Gruenberg, Jean (2007-10-31). "The p11/S100A10 Light Chain of Annexin A2 Is Dispensable for Annexin A2 ... Annexin A2 (previously named Annexin II), the vacuolar ATPase, COP coat proteins, the N-ethylmaleimide sensitive factor, the ... Emans, N.; Gorvel, J. P.; Walter, C.; Gerke, V.; Kellner, R.; Griffiths, G.; Gruenberg, J. (1993-03-02). "Annexin II is a major ... Harder, T.; Kellner, R.; Parton, R. G.; Gruenberg, J. (1997). "Specific release of membrane-bound annexin II and cortical ...
Antiphospholipid syndrome
Annexin A5 forms a shield around negatively charged phospholipid molecules, thus reducing their availability for coagulation. ... Thus, anti-annexin A5 antibodies increase phospholipid-dependent coagulation steps. The lupus anticoagulant antibodies are ...
Glucocorticoid
... annexin-1) synthesis. Lipocortin-1 both suppresses phospholipase A2, thereby blocking eicosanoid production, and inhibits ...
Browsing EMRO Regional Committee for the Eastern Mediterranean by Subject "Annexins"
Annexin-1 Mimetic Peptide Ac2-26 Suppresses Inflammatory Mediators in LPS-Induced Astrocytes and Ameliorates Pain...
Ac2-26, a mimetic peptide of Annexin-A1, plays a vital role in the anti-inflammatory response mediated by astrocytes. In this ... Annexin-1 Mimetic Peptide Ac2-26 Suppresses Inflammatory Mediators in LPS-Induced Astrocytes and Ameliorates Pain ... Annexin-1 Mimetic Peptide Ac2-26 Suppresses Inflammatory Mediators in LPS-Induced Astrocytes and Ameliorates Pain ... Annexin-1 Mimetic Peptide Ac2-26 Suppresses Inflammatory Mediators in LPS-Induced Astrocytes and Ameliorates Pain ...
Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death | Protocol (Translated to Portuguese)
Activated protein kinase C alpha associates with annexin VI from skeletal muscle | Garvan Institute of Medical Research
While other annexin isoforms may be PKC substrates or inhibitors, annexin VI phosphorylation by PKC alpha could not be detected ... Annexin VI was also detected in immunoprecipitates of PKC alpha. Annexin VI and PKC alpha are both calcium-dependent ... Annexin VI is a major skeletal muscle protein and our data are consistent with a role for this isoform in the control of ... we were also able to detect annexin VI interaction with PKC beta by overlay of skeletal muscle cytosol, but not with PKC theta ...
ANXA9 Human-Annexin
The annexins are a family of calcium-dependent phospholipid-binding proteins. Members of the annexin family contain 4 internal ... Annexin-9 althoµgh stable at 4°C for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended ... Annexin-9 at a concentration of 0.1mg containing 10mM Tris, pH 8.0, 0.1% Triton X-100 and 0.002% NaN3. ... The most striking feature of annexin 31 is a complete ablation of all four homologous type II calcium-binding sites in the ...
annexin I | Ligand page | IUPHAR/BPS Guide to IMMUNOPHARMACOLOGY
Platelet annexin V: the ins and outs. - Oxford Cardiovascular Science
Annexins are highly conserved calcium-binding proteins, of which annexin V is the major annexin in human platelets. The last ... This review distinguishes between the potential intracellular role of annexin V in platelet activation, and the uses of annexin ... Additionally, annexin V has proven very useful as a marker for apoptosis and platelet activation. It also has potential for the ... few years have seen an accumulation of information on and speculations about the roles of annexins in both intracellular and ...
Screening of an annexin-A2-targeted heptapeptide for pancreatic adenocarcinoma localization. | Mol Oncol;2022 Dec 01. |...
Structural requirements for annexin I-S100C complex-formation<...
Using a proteolytically truncated annexin I derivative as well as a number of N-terminal annexin I peptides in liposome co- ... Using a proteolytically truncated annexin I derivative as well as a number of N-terminal annexin I peptides in liposome co- ... Using a proteolytically truncated annexin I derivative as well as a number of N-terminal annexin I peptides in liposome co- ... Using a proteolytically truncated annexin I derivative as well as a number of N-terminal annexin I peptides in liposome co- ...
Modulation of annexin I and cyclooxygenase-2 in smokeless tobacco-induced inflammation and oral cancer - HSC
... results in cleavage of the anti-inflammatory peptide from the anti-inflammatory protein annexin I. Annexin I is produced from ... results in cleavage of the anti-inflammatory peptide from the anti-inflammatory protein annexin I. Annexin I is produced from ... results in cleavage of the anti-inflammatory peptide from the anti-inflammatory protein annexin I. Annexin I is produced from ... results in cleavage of the anti-inflammatory peptide from the anti-inflammatory protein annexin I. Annexin I is produced from ...
Browsing EMRO Regional Committee for the Eastern Mediterranean by Subject
Ann Kari Grindheim | Universitetet i Bergen
Vis forfatter(e) (2016). Annexin A7 and Annexin A11 as mRNA-binding Proteins. ... Vis forfatter(e) (2021). Annexin A2 binds the internal ribosomal entry site of c-myc mRNA and regulates its translation. RNA ... Vis forfatter(e) (2019). Two tales of Annexin A2 knock-down: One of compensatory effects by antisense RNA and another of a ... Vis forfatter(e) (2013). Domains I and IV of annexin A2 affect the formation and integrity of in vitro capillary-like networks. ...
The role of Annexin II in airway epithelial wound repair and the effect of corticosteroids on the Annexin II regulated pathway ...
Upregulation of annexin A1 in reactive astrocytes and its subtle induction in microglia at the boundaries of human brain...
T1 - Upregulation of annexin A1 in reactive astrocytes and its subtle induction in microglia at the boundaries of human brain ... Upregulation of annexin A1 in reactive astrocytes and its subtle induction in microglia at the boundaries of human brain ... Upregulation of annexin A1 in reactive astrocytes and its subtle induction in microglia at the boundaries of human brain ... Upregulation of annexin A1 in reactive astrocytes and its subtle induction in microglia at the boundaries of human brain ...
Measurement of phosphatidylserine exposure during storage of platelet concentrates using the novel probe lactadherin: a...
However, recent comparison of annexin V with the new calcium-independent PS probe lactadherin revealed that annexin V requires ... However, recent comparison of annexin V with the new calcium-independent PS probe lactadherin revealed that annexin V requires ... However, recent comparison of annexin V with the new calcium-independent PS probe lactadherin revealed that annexin V requires ... However, recent comparison of annexin V with the new calcium-independent PS probe lactadherin revealed that annexin V requires ...
Lipocortin 1 (Annexin 1): A Candidate Paracrine Agent Localized in Pituitary Folliculo-Stellate Cells1. - Department of...
Ultrasonic Nanoemulsification of Cuminum cyminum Essential Oil and Its | IJN
... and apoptosis through Annexin V-FITC assay. Agar well diffusion was used to study the antimicrobial activity, and this was ... The assay demonstrated early apoptosis (Annexin V+/PI−) in the SAS cell line (Figure 9A and B). Slight necrosis (Annexin V−/PI+ ... In due time, these cells were resuspended with Annexin V binding buffer. One microliter of Annexin V-FITC conjugate and 12.5 µL ... Annexin V-FITC Assay. SAS cell line (1 x 105) was cultured overnight after it was plated onto 25-cm2 flasks. Subsequently, it ...
Induction of Inflammation by West Nile virus Capsid through the Caspase-9 Apoptotic Pathway - Volume 8, Number 12-December 2002...
TUNEL Assay and Annexin V Staining. In vitro apoptosis in individual cells was determined by terminal desoxyriboxyl- ... Experiments with annexin V staining revealed that 22.9% of WNVCp-transfected cells undergo phosphatidylserine dislocalization, ... Apoptosis induction by the expression of capsid was also determined by annexin V staining procedure followed by fluorescence- ... Forty-eight hours after transfection, the cells were stained with phycoerythrin-conjugated annexin V. Only EGFP-expressing ...
ER - lysosome contacts at a pre-axonal region regulate axonal lysosome availability | Nature Communications
Toxicity mechanisms of onion (Allium cepa) extracts and compounds in multidrug resistant erythroleukemic cell line
Annexin V-FITC+/PI- cells were counted as early apoptosis; Annexin V-FITC+/PI+ and Annexin V-FITC-/PI+ cells were counted as ... Detection of apoptosis/necrosis by annexin-V/PI staining. Quantitative determination of apoptotic and/or necrotic cells was ... plus 40 µl of Annexin V-FITC solution diluted in binding buffer (1:10) and incubated for 20 min in the dark. PI (2.5 µL ) was ... performed after incubation with 1, 2 and 4 mg/ml of OE for 24 h through a reaction with Annexin V-FITC and Propidium Iodide (PI ...
JCI -
Kidney injury molecule-1 is a phosphatidylserine receptor that confers a phagocytic phenotype on epithelial cells
ATP-Glo™ Bioluminometric Cell Viability Assay - Biotium
WST-1 Assay Reagent - Cell Proliferation (ready to use) (ab155902) | Abcam
Cancers | Free Full-Text | Serine Biosynthesis Pathway Supports MYC-miR-494-EZH2 Feed-Forward Circuit Necessary to Maintain...
Cerebral Venous Thrombosis: Background, Etiology, Epidemiology
positive regulation of prostaglandin biosynthetic process Antibodies | Invitrogen
...
Kaempferol induces cell cycle arrest and apoptosis in renal cell carcinoma through EGFR/p38 signaling
The cells were collected and subjected to Annexin V and propidium iodide (PI) staining using an Annexin V-FITC Apoptosis ... 4A) showed Annexin V positive cells increased after treatment with kaempferol and there are significant differences compared ... B) The quantification for Annexin V positive cells. (C) After similar treatment, total lysates were analyzed to detect p53 (1: ... Annexin V-FITC Apoptosis Detection kit was from Nanjing Jiancheng Bioengineering Institute. ...
ProteinCorrelates with poorProteins annexinsExtracellularUpregulationCalcium-bindingApoptosisPeptideWestern BlotLigandANXA5RecombinantDifferentiallyBindsRepeatsMRNAANXA8BiomarkerInflammationPhosphorylationInteractionsHomologousRoleSerumDetectGeneticGeneVitroHumanMagneticMetastasisConcentrationExpressionOralClinicalPrognosisProbeFamilyFunctionDiseasesResources
Protein14
- Tagging Annexin V with a fluorophore produces a probe that acts similarly to native Annexin V and preserves the mass, high affinity, and specificity of the protein. (nih.gov)
- Annexin A4 (ANXA4), also known as Lipocortin IV, placental anticoagulant protein II, and zymogen granule membrane-associated protein of 36 kDa (ZAP36), is a member of the Annexin protein family. (rndsystems.com)
- In human osteoclasts, annexin A5 and annexin-binding protein S100A4 regulated fusogenic activity of syncytin 1, another protein that we show to be directly involved in the fusion event. (nih.gov)
- We have previously detected a number of protein kinase C (PKC) alpha-binding proteins in skeletal muscle cytosol by blot overlay assay, and now identify the major, 69 kDa binding protein as annexin VI by immunoblotting and overlay assay of hydroxyapatite chromatography fractions. (garvan.org.au)
- Annexin VI is a major skeletal muscle protein and our data are consistent with a role for this isoform in the control of calcium-dependent PKC. (garvan.org.au)
- Annexin A5 is a naturally occurring protein that has shown to protect, repair and improve the body's own ability to fight against unwanted inflammation. (biovian.com)
- The Company's biological drug candidate ANXV - a human recombinant protein, Annexin A5 - is intended primarily for the acute treatment of patients with cardiovascular diseases with vascular damages and inflammation. (biovian.com)
- In one type of disease that affects both skeletal and cardiac muscle, a gene mutation alters the shape of a protein called dysferlin, which normally binds annexin proteins that, as noted in the video, play a vital role in patching holes. (nih.gov)
- Annexin-9 althoµgh stable at 4°C for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. (neobiolab.com)
- The basis of the magnetic separation is very ingenious: the spermatozoids are placed in contact with metal microspheres coated with a substance called Annexin , a protein that is able to detect defective spermatozoids and fasten onto them. (clinicafertia.com)
- This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. (gentaur-shop.com)
- This analysis indicates that the local environment of the tryptophan in annexin I becomes less aqueous on S100C binding, suggesting a hydrophobic nature of the protein-protein interaction. (elsevierpure.com)
- Exposure of hamster cheek pouches to smokeless tobacco extract (STE) results in cleavage of the anti-inflammatory peptide from the anti-inflammatory protein annexin I. Annexin I is produced from cultured oral epithelial cells and its expression is modulated by STE. We further show that STE exposure of oral epithelial cells results in upregulation of the pro-inflammatory protein COX-2. (unthsc.edu)
- Protein phosphorylation and its role in the regulation of Annexin A2 function. (uib.no)
Correlates with poor2
Proteins annexins1
- Fusion also depended on cell-surface associated phosphatidylserine-binding proteins annexins (A1 and A5 for murine cells and A5 for human cells). (nih.gov)
Extracellular3
- The calcium-binding sites are required for annexins to aggregate and cooperatively bind anionic phospholipids and extracellular matrix proteins. (neobiolab.com)
- The last few years have seen an accumulation of information on and speculations about the roles of annexins in both intracellular and extracellular locations. (ox.ac.uk)
- Extracellular vesicles released from cells exposed to reactive oxygen species increase annexin A2 expression and survival of target cells exposed to the same conditions. (uib.no)
Upregulation2
- Retinal neovascularization is a common pathological change in multiple diseases of the eyes and the upregulation of annexin A2 (A2) under a hypoxic and ischemic microenvironment has been demonstrated to be a key factor in the pathological process. (spandidos-publications.com)
- In summary, we find that exposure to smokeless tobacco results in loss of the anti-inflammatory activity of annexin I and upregulation of the pro-inflammatory COX-2 in oral cells. (unthsc.edu)
Calcium-binding1
- Annexins are highly conserved calcium-binding proteins, of which annexin V is the major annexin in human platelets. (ox.ac.uk)
Apoptosis5
- Imaging of apoptosis through PS exposure using Annexin V removes the need for cellular internalization. (nih.gov)
- Annexins are a family of proteins involved in a wide variety of cellular processes such as inflammation , proliferation, differentiation, apoptosis , migration and membrane repair. (bvsalud.org)
- Additionally, annexin V has proven very useful as a marker for apoptosis and platelet activation. (ox.ac.uk)
- This review distinguishes between the potential intracellular role of annexin V in platelet activation, and the uses of annexin V in particular to detect apoptosis. (ox.ac.uk)
- We will assess lipotoxicity by measuring ceramides (mass spectrometry) and apoptosis (Annexin V, TUNEL). (nih.gov)
Peptide3
- Annexin-1 Mimetic Peptide Ac2-26 Suppresses Inflammatory Mediators in LPS-Induced Astrocytes and Ameliorates Pain Hypersensitivity in a Rat Model of Inflammatory Pain. (iasp-pain.org)
- Ac2-26, a mimetic peptide of Annexin-A1, plays a vital role in the anti-inflammatory response mediated by astrocytes. (iasp-pain.org)
- Interaction between S100C and an N-terminal annexin I peptide containing a tryptophan at position 11 can also be monitored by fluorescence emission spectroscopy after tryptophan excitation. (elsevierpure.com)
Western Blot1
- Detection of Human, Mouse, and Rat Annexin A4 by Western Blot. (rndsystems.com)
Ligand1
- Effect of serine phosphorylation and ser25 phospho-mimicking mutations on nuclear localisation and ligand interactions of annexin A2. (uib.no)
ANXA54
- Description: A sandwich quantitative ELISA assay kit for detection of Bovine Annexin A5 (ANXA5) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. (tissue-cell-culture.com)
- Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Annexin A5 (ANXA5) in serum, plasma and other biological fluids. (tissue-cell-culture.com)
- 5. Correlation of single nucleotide polymorphisms in the promoter region of the ANXA5 (annexin A5) gene with recurrent miscarriages in women of Greek origin. (nih.gov)
- 11. A common haplotype of the annexin A5 (ANXA5) gene promoter is associated with recurrent pregnancy loss. (nih.gov)
Recombinant2
- Annexin Pharmaceuticals drug candidate ANXV is a human recombinant Annexin A5. (biovian.com)
- The project completed for Annexin Pharmaceuticals falls exactly into the key strengths of our comprehensive service offering for the microbially expressed recombinant proteins. (biovian.com)
Differentially3
- The differentially expressed Annexins in RCC compared with normal controls were screened applying the TCGA database. (bvsalud.org)
- The correlation of differentially expressed Annexins with clinical stages, grades and overall survival was analyzed to explore the clinical significance of Annexins in RCC. (bvsalud.org)
- The annexins are differentially distributed in vertebrate tissues (and lower eukaryotes) and appear to be involved in MEMBRANE FUSION and SIGNAL TRANSDUCTION . (nih.gov)
Binds1
- Annexin A2 binds the internal ribosomal entry site of c-myc mRNA and regulates its translation. (uib.no)
Repeats1
- The C-terminal domain is comprised of four 60‑70 aa annexin repeats which form a tightly packed disc known as the annexin domain. (rndsystems.com)
MRNA3
ANXA81
- Potential prognostic and therapeutic value of ANXA8 in renal cell carcinoma: based on the comprehensive analysis of annexins family. (bvsalud.org)
Biomarker1
- Salivary annexin A1: A candidate biomarker for periodontitis. (nih.gov)
Inflammation1
- Annexins are also known to play a role in membrane repair and in inflammation. (nih.gov)
Phosphorylation3
- While other annexin isoforms may be PKC substrates or inhibitors, annexin VI phosphorylation by PKC alpha could not be detected after co-purification, while phosphorylation of subsequently-added histone IIIS was readily observed. (garvan.org.au)
- Reactive oxygen species exert opposite effects on Tyr23 phosphorylation of the nuclear and cortical pools of annexin A2. (uib.no)
- Phosphorylation of Annexin A2. (uib.no)
Interactions1
- Members of the Annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways. (rndsystems.com)
Homologous2
- The Annexins consist of a unique N-terminal domain followed by a homologous C-terminal core domain containing the phospholipid-binding sites. (rndsystems.com)
- The most striking feature of annexin 31 is a complete ablation of all four homologous type II calcium-binding sites in the conserved tetrad core. (neobiolab.com)
Role4
- The Multifaceted Role of Annexin A1 in Viral Infections. (nih.gov)
- However, the role of most Annexins in renal cell carcinoma (RCC) remained unclear. (bvsalud.org)
- 13. Further insights into the role of the annexin A5 M2 haplotype as recurrent pregnancy loss factor, assessing timing of miscarriage and partner risk. (nih.gov)
- 17. Role of Annexin A1 in Squamous Cell Lung Cancer Progression. (nih.gov)
Serum1
- 14. Expression of annexin A5 in serum and tumor tissue of patients with colon cancer and its clinical significance. (nih.gov)
Detect1
- thirdly, we were also able to detect annexin VI interaction with PKC beta by overlay of skeletal muscle cytosol, but not with PKC theta, the major novel PKC in this tissue, suggesting sequences specific to calcium-dependent PKC isoenzymes are involved. (garvan.org.au)
Genetic1
- 2. Genetic variations and haplotypes in the annexin A5 gene are associated with the risk of recurrent pregnancy loss. (nih.gov)
Gene2
Vitro1
- Domains I and IV of annexin A2 affect the formation and integrity of in vitro capillary-like networks. (uib.no)
Human5
- Hier sind Annexin IV ELISA Kits für eine Vielzahl von Species wie anti-Human Annexin IV, anti-Cow Annexin IV, anti-Dog Annexin IV zu finden. (antikoerper-online.de)
- Detects human Annexin A4 in direct ELISAs and Western blots. (rndsystems.com)
- PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Annexin A4 Monoclonal Antibody (Catalog # MAB4146) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007 ). (rndsystems.com)
- Annexin A4 in Human Kidney. (rndsystems.com)
- Annexin A4 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Annexin A4 Monoclonal Antibody (Catalog # MAB4146) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013 ). (rndsystems.com)
Magnetic2
- This in vivo imaging method, which has increased in popularity in recent years, has led to the development of both fluorescent and magnetic annexins for various applications (7-9). (nih.gov)
- Thus, when a magnetic field is applied to the sample, the spermatozoids to which the Annexin has bonded can be separated from the optimum spermatozoids, which will be used in the treatment. (clinicafertia.com)
Metastasis1
- 4. Brain microvascular endothelium induced-annexin A1 secretion contributes to small cell lung cancer brain metastasis. (nih.gov)
Concentration1
- Annexin-9 at a concentration of 0.1mg containing 10mM Tris, pH 8.0, 0.1% Triton X-100 and 0.002% NaN3. (neobiolab.com)
Expression1
- 7. Decreased expression of Annexin A1 correlates with pathologic differentiation grade in oral squamous cell carcinoma. (nih.gov)
Oral1
- 8. Annexin A1 down-regulation in oral squamous cell carcinoma correlates to pathological differentiation grade. (nih.gov)
Clinical3
- Annexin Pharmaceuticals AB and CDMO Biovian Oy released the First-In-Class ANXV drug candidate for clinical trials. (biovian.com)
- Biovian provided Annexin Pharmaceuticals with the ANXV Drug Substance followed by Drug Product manufacturing including aseptic filling, quality control analyses and Qualified Person certified release for use in the clinical trial. (biovian.com)
- Annexin Pharmaceuticals initiated the first clinical trial with ANXV in the Netherlands in late 2020, with the dosing of the first study participant on 21 December. (biovian.com)
Prognosis1
- Multiple members of Annexins were abnormally expressed and associated with the prognosis of RCC. (bvsalud.org)
Probe1
- Hier sind Annexin IV ELISA Kits mit einem spezifischen Probe zu finden. (antikoerper-online.de)
Family5
- Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. (antikoerper-online.de)
- Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. (antikoerper-online.de)
- The annexins are a family of calcium-dependent phospholipid-binding proteins. (neobiolab.com)
- Members of the annexin family contain 4 internal repeat domains, each of which includes a type II calcium-binding site. (neobiolab.com)
- These proteins belong to a large family of proteins called annexins. (nih.gov)
Function3
- The Annexin Homepage is dedicated to the research into the structure and function of the Annexin proteins. (structuralchemistry.org)
- Althoµgh all 4 type II calcium-binding sites in annexin 31 contained amino acid substitutions that ablated their function, structural analysis sµggests that the conserved putative ion channel formed by the tetrad core is intact. (neobiolab.com)
- Mutations of Serine 25 of Annexin A2 and their Effects on Structure and Function. (uib.no)
Diseases1
- Annexin Pharmaceuticals AB (publ) is a world-leading biotechnology company in the Annexin A5 field, for treatment of various cardiovascular diseases, which are currently the most common cause of death. (biovian.com)
Resources1
- At present, the main resources on this site are Annexins Worldwide , a directory of research laboratories in the annexin field, and the curated Annexin Sequence Database . (structuralchemistry.org)
