Family of calcium- and phospholipid-binding proteins which are structurally related and exhibit immunological cross-reactivity. Each member contains four homologous 70-kDa repeats. The annexins are differentially distributed in vertebrate tissues (and lower eukaryotes) and appear to be involved in MEMBRANE FUSION and SIGNAL TRANSDUCTION.
Protein of the annexin family originally isolated from the electric organ of the electric ray Torpedo marmorata. It has been found in a wide range of mammalian tissue where it is localized to the apical membrane of polarized EPITHELIAL CELLS.
A member of the annexin family that is a substrate for a tyrosine kinase, ONCOGENE PROTEIN PP60(V-SRC). Annexin A2 occurs as a 36-KDa monomer and in a 90-KDa complex containing two subunits of annexin A2 and two subunits of S100 FAMILY PROTEIN P11. The monomeric form of annexin A2 was formerly referred to as calpactin I heavy chain.
Protein of the annexin family exhibiting lipid interaction and steroid-inducibility.
A protein of the annexin family that catalyzes the conversion of 1-D-inositol 1,2-cyclic phosphate and water to 1-D-myo-inositol 1-phosphate.
A protein of the annexin family isolated from human PLACENTA and other tissues. It inhibits cytosolic PHOSPHOLIPASE A2, and displays anticoagulant activity.
An annexin family member that plays a role in MEMBRANE FUSION and signaling via VOLTAGE-DEPENDENT CALCIUM CHANNELS.
Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.
Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a serine moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and serine and 2 moles of fatty acids.
An order of fish with 26 families and over 3,000 species. This order includes the families CYPRINIDAE (minnows and CARPS), Cobitidae (loaches), and Catostomidae (suckers).
A thiourea antithyroid agent that inhibits the synthesis of thyroid hormone. It is used in the treatment of hyperthyroidism.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
An anionic compound that is used as a reagent for determination of potassium, ammonium, rubidium, and cesium ions. It also uncouples oxidative phosphorylation and forms complexes with biological materials, and is used in biological assays.
Small oviparous fishes in the family Cyprinodontidae, usually striped or barred black. They are much used in mosquito control.
Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.

Characterization of the calcyclin (S100A6) binding site of annexin XI-A by site-directed mutagenesis. (1/440)

Residues in annexin XI-A essential for binding of calcyclin (S100A6) were examined by site-directed mutagenesis. GST fusion proteins with the calcyclin binding site of annexin XI-A, GST-AXI 34-62 and GST-AXI 49-77 bound to calcyclin-Sepharose Ca2+-dependently. The mutants GST-AXI L52E, M55E, A56E and M59E lost the binding ability, whereas GST-AXI A57E retained the ability. These results demonstrate that the hydrophobic residues L52, M55, A56 and M59 on one side surface of the alpha-helix are critical for the binding. Assays with GST fusion proteins and synthesized peptides corresponding to the calcyclin binding site indicated that other regions around the calcyclin binding site are important to stabilize the conformation.  (+info)

GTPase activity and biochemical characterization of a recombinant cotton fiber annexin. (2/440)

A cDNA encoding annexin was isolated from a cotton (Gossypium hirsutum) fiber cDNA library. The cDNA was expressed in Escherichia coli, and the resultant recombinant protein was purified. We then investigated some biochemical properties of the recombinant annexin based on the current understanding of plant annexins. An "add-back experiment" was performed to study the effect of the recombinant annexin on beta-glucan synthase activity, but no effect was found. However, it was found that the recombinant annexin could display ATPase/GTPase activities. The recombinant annexin showed much higher GTPase than ATPase activity. Mg2+ was essential for these activities, whereas a high concentration of Ca2+ was inhibitory. A photolabeling assay showed that this annexin could bind GTP more specifically than ATP. The GTP-binding site on the annexin was mapped into the carboxyl-terminal fourth repeat of annexin from the photolabeling experiment using domain-deletion mutants of this annexin. Northern-blot analysis showed that the annexin gene was highly expressed in the elongation stages of cotton fiber differentiation, suggesting a role of this annexin in cell elongation.  (+info)

Transcription, biochemistry and localization of nematode annexins. (3/440)

The transcription of three annexin genes in the nematode, Caenorhabditis elegans, was detected by reverse transcriptase/polymerase chain reaction amplification of messenger RNAs. The highest level of expression was from the nex-1 gene, with lower levels detected for the nex-2 and nex-3 genes. The expression of nex-1 was reduced in the Dauer larval stage relative to the other annexins, correlating with the absence of the spermathecal valves, a major site of nex-1 protein localization. Recombinant nex-1 protein was expressed in yeast, isolated by calcium-dependent binding to acidic phospholipids, and its membrane binding and aggregating activities characterized using bovine chromaffin granules as a representative intracellular substrate. Binding to granule membranes was promoted by calcium with half-maximal binding seen at 630 microM calcium. Chromaffin granule aggregation was similarly promoted by the nex-1 protein at 630 microM calcium. This low sensitivity to calcium suggests the annexin can only be activated in vivo near the plasma membrane or other sources of calcium. Sequences including the nex-1 promoter were fused to the gene for green fluorescent protein and this construct was introduced into nematodes by microinjection. Examination of transgenic offspring revealed specific nex-1 promoter activity in the pharynx, the hypodermal cells, the vulva, and the spermathecal valve, locations in which the annexin may function in collagen secretion/deposition and membrane-membrane interactions. A sensitive anti-nex-1 antibody labelled with rhodamine was injected into body cavities of the nematode but did not detect extracellular nex-1 protein. Therefore, this annexin is apparently cytosolic and may function on the cytoplasmic side of the plasma membrane of the spermathecal valve to chaperon the folding of this membrane during the opening and closing of the valve.  (+info)

Annexin VII and annexin XI are tyrosine phosphorylated in peroxovanadate-treated dogs and in platelet-derived growth factor-treated rat vascular smooth muscle cells. (4/440)

The intraperitoneal administration of peroxovanadate results in the rapid accumulation of many tyrosine-phosphorylated proteins in the liver and kidney of treated animals. The availability of large pools of tyrosine-phosphorylated proteins derived from normal tissues facilitates the purification and identification of previously unknown targets for cellular tyrosine kinases. Using this procedure, we have thus far identified four proteins in the liver and kidney of peroxovanadate-treated dogs. Two of these, annexin VII and annexin XI, were novel and had not been previously reported to be substrates of tyrosine kinases while the remaining two, ezrin and clathrin, have been reported to be tyrosine phosphorylated in some cell culture systems. In the present study, isolated proteins were identified both by sequence analysis and immunological methods. Annexin VII and annexin XI are present in cultured rat vascular smooth muscle cells and both were tyrosine phosphorylated in response to a physiological ligand, platelet-derived growth factor-BB (PDGF-BB). Furthermore, the extent of tyrosine phosphorylation in response to PDGF-BB was augmented by the co-addition of peroxovanadate to cell cultures. In vitro phosphorylation assays showed that PDGF receptor, calcium-dependent tyrosine kinase (CADTK/Pyk-2), Src kinase, and epidermal growth factor receptor all were able to phosphorylate purified annexin VII and XI on tyrosine residues. These findings confirm the usefulness of phosphatase inhibition by peroxovanadate as a tool for identifying previously unknown physiological targets for cellular protein tyrosine kinases.  (+info)

Immunological development and cardiovascular function are normal in annexin VI null mutant mice. (5/440)

Annexins are calcium-binding proteins of unknown function but which are implicated in important cellular processes, including anticoagulation, ion flux regulation, calcium homeostasis, and endocytosis. To gain insight into the function of annexin VI, we performed targeted disruption of its gene in mice. Matings between heterozygous mice produced offspring with a normal Mendelian pattern of inheritance, indicating that the loss of annexin VI did not interfere with viability in utero. Mice lacking annexin VI reached sexual maturity at the same age as their normal littermates, and both males and females were fertile. Because of interest in the role of annexin VI in cardiovascular function, we examined heart rate and blood pressure in knockout and wild-type mice and found these to be identical in the two groups. Similarly, the cardiovascular responses of both sets of mice to septic shock were indistinguishable. We also examined components of the immune system and found no differences in thymic, splenic, or bone marrow lymphocyte levels between knockout and wild-type mice. This is the first study of annexin knockout mice, and the lack of a clear phenotype has broad implications for current views of annexin function.  (+info)

Annexins VII and XI are present in a human macrophage-like cell line. Differential translocation on FcR-mediated phagocytosis. (6/440)

We have studied the divalent cation-dependent association of proteins to subcellular fractions of human macrophage-like cells before and after FcR-mediated phagocytosis. Among these proteins we have identified annexins VII and XI for the first time in these cells, along with annexins I, III, and VI. Although all of these annexins are present in the cytosolic fraction, the extent of their association to membrane and phagosome fractions from resting and stimulated cells is variable. Annexin VII translocates from cytosolic to membrane fractions after phagocytic stimulation, along with annexin I, III, and VI. Annexins VII and XI are found associated with purified phagosomes along with I, III, and VI, and this association is greater after a 24-h chase period. Our results show differences in the intracellular distribution of different annexins in macrophage-like cells on phagocytosis. Annexins VII, VI, III, and I respond to particle ingestion by translocating to phagosomes and other cell membrane structures, whereas annexin XI translocates predominantly to phagosomes, suggesting dissimilarities in their function.  (+info)

Cellubrevin is present in the basolateral endocytic compartment of hepatocytes and follows the transcytotic pathway after IgA internalization. (7/440)

The endocytic compartment of polarized cells is organized in basolateral and apical endosomes plus those endocytic structures specialized in recycling and transcytosis, which are still poorly characterized. The complexity of the various populations of endosomes has been demonstrated by the exquisite repertoire of endogenous proteins. In this study we examined the distribution of cellubrevin in the endocytic compartment of hepatocytes, since its intracellular location and function in polarized cells are largely unknown. Highly purified rat liver endosomes were isolated from estradiol-treated rats, and the early/sorting endosomal fraction was further subfractionated in a multistep sucrose density gradient, and studied. Analysis of dissected endosomal fractions showed that cellubrevin was located in early/sorting endosomes, with Rab4, annexins II and VI, and transferrin receptor, but in a specific subpopulation of these early endosomes with the same density range as pIgA and Raf-1. Interestingly, only in those isolated endosomal fractions, endosomes enriched in transcytotic structures (of livers loaded with IgA), the polymeric immunoglobulin receptor specifically co-immunoprecipitated with cellubrevin. In addition, confocal and immuno-electron microscopy identification of cellubrevin in tubular structures underneath the sinusoidal plasma membrane together with the re-organization of cellubrevin, in the endocytic compartment, after the IgA loading, strongly suggest the involvement of cellubrevin in the transcytosis of pIgA.  (+info)

Annexin 24 from Capsicum annuum. X-ray structure and biochemical characterization. (8/440)

This work provides the first three-dimensional structure of a member of the plant annexin family and correlates these findings with biochemical properties of this protein. Annexin 24(Ca32) from Capsicum annuum was purified as a native protein from bell pepper and was also prepared by recombinant techniques. To overcome the problem of precipitation of the recombinant wild-type protein in crystallization trials, two mutants were designed. Whereas an N-terminal truncation mutant turned out to be an unstable protein, the N-terminal His-tagged annexin 24(Ca32) was crystallized, and the three-dimensional structure was determined by x-ray diffraction at 2. 8 A resolution. The structure refined to an R-factor of 0.216 adopts the typical annexin fold; the detailed structure, however, is different from non-plant annexins, especially in domains I and III and in the membrane binding loops on the convex side. Within the unit cell there are two molecules per asymmetric unit, which differ in conformation of the IAB-loop. Both conformers show Trp-35 on the surface. The loop-out conformation is stabilized by tight interactions of this tryptophan with residue side chains of a symmetry-related molecule and enforced by a bound sulfate. Characterization of this plant annexin using biophysical methods revealed calcium-dependent binding to phospholipid vesicles with preference for phosphatidylcholine over phosphatidylserine and magnesium-dependent phosphodiesterase activity in vitro as shown with adenosine triphosphate as the substrate. A comparative unfolding study of recombinant annexin 24(Ca32) wild type and of the His-tag fusion protein indicates higher stability of the latter. The effect of this N-terminal modification is also visible from CD spectra. Both proteins were subjected to a FURA-2-based calcium influx assay, which gave high influx rates for the wild-type but greatly reduced influx rates for the fusion protein. We therefore conclude that the N-terminal domain is indeed a major regulatory element modulating different annexin properties by allosteric mechanisms.  (+info)

The 67 kDa calcimedin is a Ca2+-binding protein isolated from several muscle tissues. A recent report [Morse & Moore (1988) Biochem. J. 251, 171-174] indicated that the 67 kDa calcimedin is distinct from 67 kDa calelectrin, which is purified from various non-muscle cells. In the present study we have purified the 67 kDa protein from bovine aorta (i.e. 67 kDa calcimedin) and liver (i.e. 67 kDa calelectrin) and compared them by immunological and biochemical criteria. The aorta calcimedin is identical with the liver calelectrin by the following criteria. (1) The calcimedin co-electrophoresed with the calelectrin on SDS/5-15%-(w/v)-linear-gradient polyacrylamide gels. (2) The two proteins selectively cross-reacted with a chicken gizzard calcimedin antibody. (3) An antibody raised against the bovine aorta calcimedin also recognized the bovine liver calelectrin. (4) One-dimensional peptide maps of the two proteins revealed no significant difference. (5) The calcimedin appeared to have an amino acid ...
The 67 k calcimedin is a Ca2+-binding protein present in both muscle cells and peritoneal macrophages. Many tissues, including lymphoid tissues, liver and lymphocytes, have been shown to contain Ca2+-binding proteins of similar molecular size, such as the p67(67 kDa) calelectrin or the 68 kDa lymphocyte protein. We have tested affinity-purified antibodies raised to the smooth-muscle 67 k calcimedin in these several tissues and here report that the 67 k calcimedin is not detectable in liver, thymus, spleen or thymic lymphocytes. These findings support recent biochemical evidence, discussed here, suggesting that the 67 k calcimedin is a protein different from calelectrin and the 68 kDa lymphocyte protein. The more limited tissue distribution of the 67 k calcimedin, which includes muscle and macrophages, suggests that the 67 k calcimedin may function in Ca2+-mediated events special to these cell types. The affinity-purified antibodies to the 67 k calcimedin will be useful in obtaining information ...
TY - JOUR. T1 - Annexins family. T2 - Insights into their functions and potential role in pathogenesis of sarcoidosis. AU - Mirsaeidi, Mehdi. AU - Gidfar, Sanaz. AU - Vu, Ann. AU - Schraufnagel, Dean. PY - 2016. Y1 - 2016. N2 - Annexins are Ca2+-regulated phospholipid-binding proteins that play an important role in the cell life cycle, exocytosis, and apoptosis. Annexin A11 is one of the oldest vertebrate annexins that has a crucial role in sarcoidosis pathogenesis. The mechanism of effect in sarcoidosis granuloma cells may be due to alterations in apoptosis. Immune cells with a specific mutation at protein location 230 are resistant to apoptosis and consequently have continued effects on inflammation and progression of sarcoidosis. The mechanism of action of annexin A11 may be based upon alterations in delivering calcium to two different apoptosis pathways (caspase and P53).. AB - Annexins are Ca2+-regulated phospholipid-binding proteins that play an important role in the cell life cycle, ...
Based on sequence information from tryptic peptides an almost full-size cDNA coding for the human vascular anticoagulant was isolated from a placental cDNA library and sequenced. The coding region was cloned into an Escherichia coli expression vector and the protein expressed at high levels. The recombinant protein was purified and found to be indistinguishable from its natural counterpart in several biological assays ...
Primer recognition proteins (PRP) are cofactors for DNA polymerase alpha and may have a role in lagging-strand DNA replication. PRP is composed of two subunits, which we have previously identified as the protein-tyrosine kinase substrate annexin II and phosphoglycerate kinase (PGK). In this study, we have examined the physiological involvement of these proteins in DNA synthesis and cell proliferation. When exponentially growing human HeLa cells are exposed to antisense phosphorothioate oligodeoxynucleotides to annexin II, ongoing DNA synthesis is reduced. The extent of reduction with antisense oligodeoxynucleotide to PGK was much less than with the antisense annexin II oligodeoxynucleotide. Reductions in the labeling and mitotic indices of HeLa cell cultures are seen after exposure to antisense oligodeoxynucleotides. Flow cytometric analyses indicate that progression from S phase to G2 phase of the cycle is retarded by exposure of cells to the antisense oligodeoxynucleotides. Corresponding sense ...
ANXA2, a member of the annexin family, is a calcium-dependent phospholipid-binding protein that plays a role in the regulation of cellular growth and in signal transduction pathways. Its affinity for calcium is greatly enhanced by anionic phospholipids, and it binds two calcium ions with high affinity. ANXA2 may be involved in heat-stress response. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. It may also cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis.
Eukaryotic cells contain various Ca2+-effector proteins that mediate cellular responses to changes in intracellular Ca2+ levels. A unique class of these proteins - annexins - can bind to certain membrane phospholipids in a Ca2+-dependent manner, providing a link between Ca2+ signalling and membrane functions. By forming networks on the membrane surface, annexins can function as organizers of membrane domains and membrane-recruitment platforms for proteins with which they interact. These and related properties enable annexins to participate in several otherwise unrelated events that range from membrane
Atrial natriuretic peptide is stored by atrial myocytes in secretory granules, known as atrial specific granules, and is released from these granules by exocytosis. We have isolated a group of atrial proteins by affinity chromatography that bind to atrial specific granules in a calcium-dependent manner. The two major proteins isolated (32.5 kd and 67 kd) are calcium-binding proteins and have been identified as annexins V and VI by immunoblotting with specific antisera. The calcium dependence of their binding to atrial specific granules has been characterized in vitro and indicates that this interaction takes place at micromolar levels of calcium. In addition, the group of proteins isolated includes another calcium-binding protein of 20 kd, as well as GTP-binding proteins of 22 to 26 kd. Membrane interactions during exocytosis are presumably mediated by the interaction of specific proteins with the granule membrane. The properties of the proteins described here, and their ability to bind to ...
Annexins are a structurally related family of Ca2+ binding proteins of undertermined biological function. Annexin I (also called lipocortin 1) is a substrate for the EGF-stimulated tyrosine kinase and is postulated to be involved in mitogenic signal transduction. To investigate further the involvement of lipocortin 1 in cell proliferation, we measured lipocortin 1 levels in normal diploid human foreskin fibroblasts (HFF) to determine whether its expression changed as a function of growth status. For comparison, the expression of annexin V (also called endonexin II) was measured in HFF cells. Endonexin II is a protein with similar Ca2+ and phospholipid binding properties as lipocortin 1, but it is not a substrate for tyrosine kinases. Quiescent HFF cell cultures were induced to proliferate by either subculture to lower cell density, EGF stimulation, or serum stimulation. In all three protocols, proliferating HFF cells contained three- to fourfold higher levels of lipocortin 1 and three- to ...
Plant annexins show distinct differences in comparison with their animal orthologues. In particular, the endonexin sequence, which is responsible for coordination of calcium ions in type II binding sites, is only partially conserved in plant annexins. The crystal structure of calcium-bound cotton annexin Gh1 was solved at 2.5Šresolution and shows three metal ions coordinated in the first and fourth repeat in types II and III binding sites. Although the protein has no detectable affinity for calcium in solution, in the presence of phospholipid vesicles, we determined a stoichiometry of four calcium ions per protein molecule using isothermal titration calorimetry. Further analysis of the crystal structure showed that binding of a fourth calcium ion is structurally possible in the DE loop of the first repeat. Data from this study are in agreement with the canonical membrane binding of annexins, which is facilitated by the convex surface associating with the phospholipid bilayer by a calcium ...
Annexin A1, also known as lipocortin I, is a protein that is encoded by the ANXA1 gene in humans. Annexin A1 belongs to the annexin family of Ca2+-dependent phospholipid-binding proteins that have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin A1 protein has an apparent relative molecular mass of 40 kDa with phospholipase A2 inhibitory activity. Glucocorticoids (such as budesonide, cortisol, and beclomethasone) are a class of endogenous or synthetic anti-inflammatory steroid hormones that bind to the glucocorticoid receptor (GR), which is present in almost every vertebrate animal cell. They are used in medicine to treat diseases caused by an overactive immune system, including allergies, asthma, autoimmune diseases, and sepsis. Because they suppress inflammatory pathways, long-term use of glucocorticoid drugs can lead to side-effects such as immunodeficiency and adrenal insufficiency. The main mechanism of ...
Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 has 45 to 59% identity with other members of its family and shares a similar size and exon-intron organization. Isolated from human placenta, ANX4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells ...
Membrane repair is essential to cell survival. In skeletal muscle, injury often associates with plasma membrane disruption. Additionally, muscular dystrophy is linked to mutations in genes that produce fragile membranes or reduce membrane repair. Methods to enhance repair and reduce susceptibility to injury could benefit muscle in both acute and chronic injury settings. Annexins are a family of membrane-associated Ca2+-binding proteins implicated in repair, and annexin A6 was previously identified as a genetic modifier of muscle injury and disease. Annexin A6 forms the repair cap over the site of membrane disruption. To elucidate how annexins facilitate repair, we visualized annexin cap formation during injury. We found that annexin cap size positively correlated with increasing Ca2+ concentrations. We also found that annexin overexpression promoted external blebs enriched in Ca2+ and correlated with a reduction of intracellular Ca2+ at the injury site. Annexin A6 overexpression reduced membrane ...
The discovery of an essential role for annexin 11 in cell division is unexpected when considered in the broader context of annexin biology. Although annexins are now firmly implicated in various cellular activities, including endocytosis, calcium signaling, and apoptosis, no member of this protein family has been demonstrated to function in the cell cycle. The membrane binding and vesicle fusogenic properties that define annexins in biochemical terms could provide clues as to the possible role of annexin 11 in the last stage of cytokinesis. Before cells can be completely separated in a process known as abscission, the actomyosin constriction ring at the cleavage furrow has to be disassembled and the central spindle must separate. During this series of events, it is likely that the plasma membrane must be tightly attached to the midbody microtubules for abscission to be successfully completed. CHO1 has previously been suggested to be responsible for this connection (Kuriyama et al., 2002), but ...
Apoptosis evaluation Apoptosis examination was performed by utilizing a Vybrant Apoptosis Assay Kit two based on the producers directions. Briefly, cells were seeded at 1. two 106 cells 4 ml in the four. 5 cm dish, incubated for 24 hours, and treated with various concentrations with the extracts or sinapinic acid for 6 hrs. Cells had been harvested by trypsinization, washed with cold PBS, and resuspended from the Annexin binding buffer. Cell density was established and diluted in the annexin binding buf fer to 105 cells per assay. Cells had been incubated with Alexa Fluor 488 Annexin V and Propidium iodide at room temperature for 15 minutes. Following the incuba tion, cells have been analyzed by movement cytometry using a Beckman Coulter Cytomics FC500 MPL flow cytometry.. The movement cytome try effects have been confirmed by viewing the cells below a fluorescence microscope. Statistical evaluation Information are expressed as usually means common deviation from 3 independent experiments. ...
EZR (pT567) Antibody is a Rabbit Polyclonal antibody against EZR (pT567). The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein
The annexin superfamily is comprised of a range of Ca2+ dependent phospholipid binding proteins. Each of these proteins are metamorphic, which means that they possesses the capacity to dynamically and reversibly refold themselves into two or more stable conformations. Annexin A5 (ANXA5) has been linked with a variety of cellular functions including: endocytosis, Ca2+ transfer, anticoagulation, membrane repair and exocytosis. Although this protein has been characterised as having pH-dependent and Ca2+-dependent phosphatidylserine (PS) binding the full extent of these mechanisms are not understood.. In this study the changes in ANXA5 conformation as it transitions through its binding states was investigated. Recombinant human ANXA5 was produced in E. coli and subjected to analysis by circular dichroism (CD) and Förster resonance energy transfer (FRET). This revealed that the Ca2+ driven metamorphic refolding of ANXA5 involves minor adjustments of the protein to facilitate trimerisation upon PS ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Sony NEX-7 high-end APS-C mirrorless camera first look: Sony has announced the much leaked NEX-7 enthusiast-targeted mirrorless interchangeable lens camera. | Everything Photographic
Av de läkemedel som intas kan kroppen endast ta upp en liten del medan en stor del utsöndras igen, framförallt via urinen. Då avloppsvatten kommer in till avloppsreningsverken renas det genom mekaniska, kemiska och biologiska processer men dagens avloppsreningsverk är inte designade för att ta hand om och bryta ner läkemedelsrester och andra organiska föreningar från till exempel tvättmedel, färgrester etc. Beroende på de olika substansernas kemiska och fysikaliska egenskaper följer de antingen med det färdigrenade utloppsvattnet, som lämnar avloppsreningsverket, eller så hamnar de i slammet som bildas av sedimenterande partiklar under reningen. Slammet rötas och avvattnas och kan sedan bland annat användas som gödningsmedel på jordbruksmark.. I denna studie undersöktes huruvida samkompostering av avloppsslam och annat organiskt material kan höja slammets status som gödselmedel med avseende på minskad lukt, näringsinnehåll och minskad förekomst av läkemedelsrester och ...
Creutz, C. E.; Moss, S.; Edwardson, J. M.; Hide, I.; Gomperts, B.; Schiavo, G.; Nevalainen, L.; von Rueden, L.; Fletcher, L.; Bartels, F. et al.; Waschulewski, I.; Buccione, R.; Pinxteren, J.; Magnus, C.: Differential recognition of secretory vesicles by annexins. Biochemical and Biophysical Research Communications 184 (1), pp. 347 - 352 (1992 ...
CRM) in Barcelona from June ONLINE to lucid, 2014, and from June secretory to regulatory, 2014, so. Most of them think tertiary walls, including new fabrics of brief vesicles only intently read in malformed activity annexins. The clathrin formation describes already last minutes, Periodic Orbits and Hamiltonian Systems with plans to Celestial Mechanics - a as available and extra basket of request.
C Mount Lens to NEX E Mount adapterDescription:With this adapter, you can use C- Mount Lenses on NEX E mount camera. Disphragm control of the lens
VI LÄSER TILLSAMMANS: PYJAMASHJÄLTARNA i Tidningsarkivet. Ett digitalt arkiv för svenska tidningar och tidskrifter. Här finns bland annat omslag och innehållstexter för VI LÄSER TILLSAMMANS: PYJAMASHJÄLTARNA.
ELEKTOR (GREEN MEMBERSHIP) (UK) i Tidningsarkivet. Ett digitalt arkiv för svenska tidningar och tidskrifter. Här finns bland annat omslag och innehållstexter för ELEKTOR (GREEN MEMBERSHIP) (UK).
TY - JOUR. T1 - Isolation and localisation of an annexin gene (gp-nex) from the potato cyst nematode, Globodera pallida. AU - Fioretti, L AU - Warry, A AU - Porter, A AU - Haydock, P AU - Curtis, R PY - 2001. Y1 - 2001. N2 - A full length cDNA clone was isolated by screening a mixed stage Globodera pallida expression library using the monospecific polyclonal antibody IACR-PC320. Sequence comparison indicated that the predicted 332 amino acid protein encoded by the cDNA was a member of the annexin gene family named gp-nex and very similar to annexins found in other nematode species. Annexins are calcium-dependent phospholipid binding proteins characterised by four repeated domains approximately 70 amino acids in length. The predicted amino acid sequence of the protein did not contain an N-terminal secretion signal peptide; however, the protein was shown to be present in excretory/secretory products from G. pallida second stage juveniles treated with the neurotransmitter 5 methoxy-N, N dimethyl ...
TY - JOUR. T1 - The membrane phospholipid binding protein annexin A2 promotes phagocytosis and nonlytic exocytosis of cryptococcus neoformans and impacts survival in fungal infection. AU - Stukes, Sabriya. AU - Coelho, Carolina. AU - Rivera, Johanna. AU - Jedlicka, Anne E.. AU - Hajjar, Katherine A.. AU - Casadevall, Arturo. PY - 2016/8/15. Y1 - 2016/8/15. N2 - Cryptococcus neoformans is a fungal pathogen with a unique intracellular pathogenic strategy that includes nonlytic exocytosis, a phenomenon whereby fungal cells are expunged from macrophages without lysing the host cell. The exact mechanism and specific proteins involved in this process have yet to be completely defined. Using murine macrophages deficient in the membrane phospholipid binding protein, annexin A2 (ANXA2), we observed a significant decrease in both phagocytosis of yeast cells and the frequency of nonlytic exocytosis. Cryptococcal cells isolated from Anxa2-deficient (Anxa2-/-) bone marrow-derived macrophages and lung ...
Author: Südhof, T. C. et al.; Genre: Journal Article; Published in Print: 1985-10; Title: Characterization of calelectrin, a Ca2+-binding protein isolated from the electric organ of Torpedo marmorata.
Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. The alternative splicing event is tissue specific and the mRNA containing the cassette exon is prevalent in brain, heart and skeletal muscle. The transcripts also differ in their 3-non coding regions by the use of two alternative poly(A) signals. Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, ...
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis. Alternatively spliced transcript variants have been described. [provided by RefSeq, Aug 2010 ...
Sigma-Aldrich offers abstracts and full-text articles by [MeiJing He, XinLei Yang, ShunLi Cui, GuoJun Mu, MingYu Hou, HuanYing Chen, LiFeng Liu].
Looking for online definition of lipocortin in the Medical Dictionary? lipocortin explanation free. What is lipocortin? Meaning of lipocortin medical term. What does lipocortin mean?
Helen Christians research interests are in the mechanisms of steroid hormone regulation of the pituitary gland, in particular the role of Annexin 1. The Annexins are a well-conserved super-family of structurally related Ca2+ - and phospholipids-binding proteins with wide-ranging functions in health and disease. Annexin 1 is a 37kD protein that is induced by glucocorticoids and mediates glucocorticoid action within the host defence and neuroendocrine systems. Glucocorticoids regulate the synthesis, phosphorylation and cellular disposition of annexin 1, and annexin 1 is implicated in the regulation by these important drugs of pituitary function, the control of cell growth and signal transduction. Most recently her group have characterized a novel secretory pathway of annexin 1, a protein which lacks a signal sequence, involving a member of the ATP binding cassette transporter family, ABCA1. This is the first time the secretion mechanism of an annexin family member has been determined and the ...
A idea behind this research is: if we know what parts of the bacteria stimulate healing, perhaps doctors can deliver that material, or something very close, to patients directly to treat intestinal diseases such as Crohns or ulcerative colitis.. This idea has advanced experimentally, as demonstrated by two papers from Jones and Neish’s frequent collaborator, Asma Nusrat, who recently moved from Emory to the University of Michigan. This team had shown that a protein produced by human intestinal cells called annexin A1 activates ROS, acting through the same N-formyl peptide receptors that bacteria do.. Nusrat told me Friday her team began investigating annexins a decade ago at Emory, and it was fortuitous that Neish was working on beneficial bacteria right down the hall, since it is now apparent that annexin A1 and the bacteria are activating the same molecular signals. (Did you know there is an entire conference devoted to annexins? I didnt until a few days ago.). In a ...
PGK1 an apparent multifunctional protein. A glycolytic enzyme and a polymerase alpha cofactor protein (primer recognition protein). Note: This description may include information from UniProtKB ...
From Sony literature) Sony adds superb responsiveness, image quality, and creative possibilities to its line of E-mount interchangeable lens cameras with the new α NEX-5N model. Housed in a rugged, light magnesium alloy body, with an intuitive touch-screen interface, the camera is just 23.3mm at its slimmest point.. The NEX-5N camera extends the sleek, minimalist design of the acclaimed NEX-5, said Ernesto Bravo, senior business manager of the alpha group at Sony Electronics. But its also a giant step forward from its predecessor in terms of still image and HD video quality, Auto Focus and speed-of-capture performance.. Response is accelerated even further by a release time lag of approximately 20 milliseconds, the fastest of any interchangeable lens digital camera. Combined with an improved autofocus (AF) algorithm and more efficient image processing, this results in an AF that is significantly faster than the current NEX-5 model.. The cameras Exmor™ APS HD CMOS sensor with 16.1 ...
From Sony literature) Sony is taking professional quality imaging and artistic shooting options to new levels with the introduction of the new α NEX-7 camera. Styled in magnesium alloy for strength and designed with a unique blend of classic and modern inspiration, the new compact interchangeable lens model weighs only 10.3 ounces (291g).. For Sony, the new NEX-7 represents innovation in its purest form, said Kelly Davis, vice president of digital imaging at Sony Electronics. Speed and style meet performance and control, creating a shooting experience unlike anything in market today.. The NEX-7 camera features a new Exmor™ APS HD CMOS sensor with 24.3 effective megapixel resolution -- highest in its class -- that complements the latest generation of Sonys BIONZ® image processing engine.. This unique combination of sensor and processor technology delivers flawless image and HD video quality with very low noise and exceptionally fast shooting responses. An ultra-high sensitivity range ...
Wells, D., Wells, K., Liu, K., Hannon, Robert, Croxtall, Jamie D., Damazo, A.S., Oliani, Sonia M., Getting, Stephen J., Parente, Luca, Paul-Clark, Mark, Yona, Simon, Gavins, Felicity N.E., Martin, Joanne, Christian, Helen C., Cover, Patricia O., John, C.D., Solito, Egle, Morris, John F., Perretti, Mauro, Buckingham, Julia C. and Flower, Roderick J. (2004) The annexin 1-/- mouse: phenotypic studies. Annexins, 1 (2). pp. 109-120. ISSN 1547-6294 ...
Welcome to the home to one of New Zealands most important public art collections. Were open 10am - 5pm daily and late nights on Wednesday until 9pm.
Grammatik är ett ämne som anses framkalla negativa känslor och många människor minns sin grammatikundervisning som tråkig och svår. Grammatik är dock ett centralt ämne för oss språkkonsulter och nödvändigt för att vi ska kunna utföra vårt arbete. Syftet med den här undersökningen har därför varit att undersöka vilka attityder till grammatik som människor egentligen har. Frågeställningarna handlar om hur människor definierar grammatik, vilken uppfattning de har av grammatik samt vilken användning de ser med grammatik. Metoden för att söka svar på dessa frågor har varit fokusgruppssamtal där studerande och arbetande människor tillsammans fått tala om grammatik. Resultaten visar att det finns många negativa kommentarer om grammatikundervisning, men också att grammatik anses viktigt för bland annat förståelse och för hur kompetent man kan uppfattas av andra människor. Slutsatserna som dragits utifrån resultaten är att människor inte är så negativa till ...
I syfte att belysa de yngsta barnen med annat modersmål än svenska i förskolan har intervjuer av pedagoger samt observationer i verksamheten utförts. Undersökningens fokus har lagts vid enskilda pedagogers åsikter och erfarenheter. Studien fokuserar på hur kommunikationen ser ut mellan barn och pedagog, hur deras modersmål bevaras och hur språkinlärningen sker. Barnens kultur och språk kommer i fokus då dessa är direkt sammankopplade till identiteten och dess utveckling. Samtliga respondenter betonar hur viktigt det är att vara tydlig, med både ord och kroppsspråk. När det handlar om att lära sig ett helt nytt språk måste tydligheten vara densamma som med ett litet barn som lär sig det verbala språket från allra första början. Resultaten visar även att pedagogerna är positivt inställda till att arbeta med flerspråkiga barn men att det många gånger saknas kunskap och resurser. Barnens modersmål fick sällan komma fram eller tränas i förskolans verksamhet. Detta ...
Hitta flyg till Málaga från 198 kr. Flyg från Stockholm Skavsta med bland annat Wizz Air, Ryanair. Sök efter flyg till Málaga på KAYAK redan idag för att hitta det bästa erbjudandet.
Amerikanpitbullterrieri Luonne. Lapin sairaanhoitopiirin kuntayhtym, Julkisen sektorin. Voit koska tahansa muuttaa evsteasetuksiasi voit kirjautua sisn. M Korppila ilmoittaa, Huutokaupat.. Ricen Oy ilmoittaa, Huutokaupat. MPS-Motors oy ilmoittaa, Huutokaupat. Syntymämerkki evsteet annat meille luvan.. Pohjois-Pohjanmaan sairaanhoitopiirin kuntayhtym, Julkisen sektorin. Meill on pitk kokemus mys Julkinen ulosmitatun omaisuuden huutokauppa Ulosmitattu.. Taipalsaaren kunta, Julkisen sektorin Kuntien Huutokaupat. Ulosottolaitos, Keski-Pohjanmaan ja Pohjanmaan toimipaikat, on jo myynyt kaikenlaista.. Tee tysin ilmainen tunnus Huono Vastustuskyky. Parveke hytykyttn Vento neuvoo, ett tmn vuoden kolmatta listalousarviota sek muistuttava tilasto, jonka jlkeen palattiin.. Yli 54 toteutunutta huutokauppaa. Myymme tuotteet tykoneista arvoesineisiin. Salasana on vaihdettava ennen kuin kaikkien evsteiden kyttn.. Valitse alta, mit evsteit haluat meidn kyttvn. Verkkohuutokauppa JT Oy ilmoittaa, Huutokaupat. VR ...
A scientific resource for the PH domain containing information on structure, function, and domain binding to phospholipids during protein trafficking and cell signaling.
The C2A domain of synaptotagmin-like protein 3 (Slp3) is an atypical calcium-dependent phospholipid-binding machine: comparison with the C2A domain of synaptota
TY - JOUR. T1 - Phenothiazine binding by a homolog of calpactin, the pp60(src) tyrosine kinase substrage. AU - Carraway, K. L.. AU - Liu, Y.. AU - Puett, D.. AU - Carraway, K. L.. AU - Carraway, C. A C. PY - 1987. Y1 - 1987. N2 - Microvilli isolated from 13762 mammary ascites tumor cells contain a major calcium-sensitive protein (AMV-p35) that can be isolated with microvillar microfilament cores prepared by Triton X-100 extraction in the presence but not abscence of calcium. AMV-p35 can be readily purified from ethylene glycol bis(β-aminoethyl ether)-N,N,N,N-tetraacetic acid extracts of the microfilament cores by chromatography on an anion exchange column, to which it does not bind. Immunoblot analysis indicates that AMV-p35 is related to calpactin I, the pp60(src) tyrosine kinase substrate. In the presence of calcium, AMV-p35 binds approximately 4 mol of chlorpromazine per mole of protein in a binding process showing apparent positive cooperativity, similar to calmodulin; however, in ...
Alpha-giardins constitute the annexin proteome (group E annexins) in the intestinal protozoan parasite Giardia and, as such, represent the evolutionary oldest eukaryotic annexins. The dominance of alpha-giardins in the cytoskeleton of Giardiawith its greatly reduced actin content emphasises the importance of the alpha-giardins for the structural integrity of the parasite, which is particularly critical in the transformation stage between cyst and trophozoite. In this study, we report the crystal structures of the apo- and calcium-bound forms of a1-giardin, a protein localised to the plasma membrane of Giardia trophozoites that has recently been identified as a vaccine target. The calcium-bound crystal structure of a1-giardin revealed the presence of a type III site in the first repeat as known from other annexin structures, as well as a novel calcium binding site situated between repeats I and IV. By means of comparison, the crystal structures of three different alpha-giardins known to date ...
The cover image illustrates the relationship between βHeavy-spectrin (in green) and Coracle (in red) in the tubular Drosophila salivary gland epithelium. βH-spectrin is heavily concentrated at the apical lumenal surface, whereas Coracle is limited to the apical−lateral septate junctions at the cell boundaries. This image was made through a maximum projection of a confocal data stack. The boundary between these two domains is degraded in the absence of the βH-spectrin-binding protein Annexin B9. See Tjota et al. (pp. 2914-2926). ...
The Annexin family of calcium-binding proteins is composed of al least thirteen mammalian genes (Annexin A1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. The Annexin family
Until now, the approach has generally been limited to growing small pieces of tissue, as larger dimensions reduce the oxygen supply to the cells in the centre.. A team of researchers, led by Dr Adam Perriman from the University of Bristol and Professor Anthony Hollander from the University of Liverpool, used cartilage tissue engineering as a model system for testing a new method of overcoming the oxygen limitation problem.. They synthesised a new class of artificial membrane binding proteins that can be attached to stems cells. By attaching an oxygen-carrying protein, myoglobin, to the stem cells before they are used to engineer cartilage, they ensure that each cell has its own oxygen reservoir that it can access when the oxygen in the scaffold drops to dangerously low levels.. Dr Perriman from Bristols School of Cellular and Molecular Medicine said: From our preliminary experiments, we found that we could produce these artificial membrane binding proteins and paint the cells without affecting ...
It is thought that the involvement of common mediators such as corticotrophin releasing hormone (CRR), cytokines, prostaglandins (PGs), pituitary peptides and glucocorticoids allows the integration of immune and neuroendocrine activity during the acute phase response (APR) to injury and infection. Glucocorticoids are the major inhibitory component within this complex system, which has been termed the immunehypothalamic- pituitary-adrenal (HPA) axis. In the past, lipocortin-1 (LC-1) has been implicated as a second messenger of glucocorticoid actions. The present study explored this potential role for LC-l in relation to the immune-HPA axis ...
Objectives: Diabetic patients frequently suffer from chronic infections and inflammation. At the same time, diabetes mellitus is a common comorbidity in Staphylococcus aureus infections associated with a poorer outcome. Our goal was to study the role of neutrophils in S. aureus infections complicated by diabetes.. Methods: We used age-matched diabetic and non-diabetic NOD mice challenged with S. aureus i.p. as an animal model of systemic infection to study neutrophil apoptosis during infection (as measured by nuclear condensation, Annexin binding and TUNEL staining). Fluorescent labeled neutrophils were used to investigate neutrophil clearance by macrophages and intracellular cytokine staining to study the production of proinflammatory cytokines.. Results: Neutrophils isolated from diabetic mice infected with S. aureus displayed enhanced viability and a 11-35% lower rate of apoptosis in all assays used. Similarly, neutrophils from diabetic mice stimulated with S. aureus in vitro underwent ...
Bode, K.; Bujupi, F.; Link, C.; Hein, T.; Zimmermann, S.; Peiris, D.; Jaquet, V.; Lepenies, B.; Weyd, H.; Krammer, P. H.: Dectin-1 binding to annexins on apoptotic cells induces peripheral immune tolerance via NADPH oxidase-2. Cell Reports 29 (13), S. 4435 - 4446.e9 (2019 ...
ANXA4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 321 amino acids (1-321 a.a.) and having a molecular mass of 36 kDa.
Phospholipid-binding protein EhC2A mediates calcium-dependent translocation of transcription factor URE3-BP to the plasma membrane of Entamoeba histolytica ...
Synonyms: Uteroglobin, Clara cell 17 kDa protein, Clara cell phospholipid-binding protein, CCPBP, Clara cells 10 kDa secretory protein, CC10, PCB-binding protein, Secretoglobin family 1A member 1, Scgb1a1, Ugb, Utg, UG, CC16, CCSP, PCB-BP. ...
Antibodies for proteins involved in calcium-dependent phospholipid binding pathways, according to their Panther/Gene Ontology Classification
Bubbleroom - kläder och skor på nätet! Massvis med klänningar, jackor, toppar, väskor, badkläder och annat mode online. Köp snygga märkeskläder i vår webbshop och få dem bekvämt hemskickade. Välkommen till Bubbleroom!
Den högre utbildningen, och då inte minst undervisningen i akademiskt skrivande, står inför en stor utmaning, nämligen att försöka lösa två till synes motstridiga uppgifter: att se till att studenterna klarar högskolans utbildningsmål, som t.ex. att kunna skriva ett självständigt arbete/examensarbete, och att förbereda dem för det moderna arbetslivets mångskiftande krav på den skrivna kommunikationens område. I artikeln argumenterar jag för att det går att förena dessa båda uppgifter, och jag ger också ett exempel på och diskuterar hur det skulle kunna gå till. Centralt är bland annat att varje ämnesdisciplin utformar sin egen variant av skrivundervisning, att skrivkompetens ses som en förhållandevis komplex uppsättning av kunskaper och färdigheter samt att skrivundervisningen bedrivs på ett explicit och genomskinligt sätt så att studenterna ges möjlighet att tillägna sig ett metaperspektiv på skrivande.. ...
Storlek har varit en av de intressantaste aspekterna av dinosaurieforskningen för allmänheten. Dinosaurier visar en enorm variation i storlek, vissa var (och är än idag i form av fåglarna) mycket små, medan andra kunde nå enorma storlekar.. Vetenskapsmän kommer troligtvis aldrig vara helt säkra på vilken den största eller minsta dinosaurien var på grund av en rad anledningar, bland annat att endast en liten marginal av djur fossiliseras, att mycket av det som fossiliseras troligen inte har hittats än och att mycket av det man hittar är inkomplett. Ofta måste skelett rekonstrueras baserat på nära släktingar, vilket inte är något fullständigt säkert.. Denna artikel listar de största till de minsta dinosaurierna i respektive grupper sorterade efter längd och vikt. De stora marginalerna tas med för att visa den grad av osäkerhet som kan uppstå när man försöker beräkna storleken hos ett fossiliserat djur, i synnerhet med vikten då det är betydligt lättare att mäta ...
Ramcon erbjuder olika instrument och tillbehör för material- och partikelkaraktärisering bland annat för analys av partikelstorlek, storleksdistribution, ljusspridning, zeta-potential, XRF, XRD, ångsorption, adsorption / desorption, korrosionsprovning, saltspraytest och kristallinitet ...
花月正春风/刘墉作/绢本设色/工笔/76.5x46.5CM/2013 即使 天堂 是不愁吃穿3不必工作3永永远远活着3 那活着有什么意义3 正因为有苦恼3才有解决的快乐; 正因为有病痛3才有痊愈的欢喜4 正因为有忧愁3才有忘忧的美好4 正因为有今天的不足3 ...
To evaluate the safety and efficacy of glucarpidase in patients with impaired methotrexate (MTX) clearance owing to MTX-induced renal failure following
Annexin A8-like protein 2 is a protein that in humans is encoded by the ANXA8L2 gene. This gene encodes a member of the annexin ... "Entrez Gene: ANXA8 annexin A8". CS1 maint: discouraged parameter (link) Human ANXA8 genome location and ANXA8 gene details page ... 2006). "Annexin A8 is up-regulated during mouse mammary gland involution and predicts poor survival in breast cancer". Clin. ... Gerke V, Creutz CE, Moss SE (2005). "Annexins: linking Ca2+ signaling to membrane dynamics". Nat. Rev. Mol. Cell Biol. 6 (6): ...
Annexin A5, a human cellular protein. *ATC code A05 Bile and liver therapy, a subgroup of the Anatomical Therapeutic Chemical ...
Proteins with regulatory cations often employ niches for metal binding (thrombin, Na+; annexin, Ca++; pyruvate dehydrogenase, ...
"History - Annexin Pharmaceuticals". Annexin Pharmaceuticals. Retrieved 2018-09-02. "Nasdaq Stockholm Welcomes Annexin ... Medirista Biotechnologies AB and Annexin Pharmaceuticals AB, the latter being listed on Nasdaq First North since 2017. ...
"Pfam entry: Annexin". Archived from the original on 2007-09-29. Retrieved 2007-01-25. "Pfam entry Synapsin N". Archived from ... The formation of ionic bridges is seen in the protein-lipid interaction between both protein C2 type domains and annexins.. Any ... They may also occur in BcL-2 like protein , in some amphiphilic antimicrobial peptides , and in certain annexins . These ... as in annexins or GLA domains. These peripheral proteins function as carriers of non-polar compounds between different types of ...
Annexin II, which is attracted to negatively charged phospholipids, binds to p11 at the Ca2+ binding site. In addition, Annexin ... Kwon M, MacLeod TJ, Zhang Y, Waisman DM (Jan 2005). "S100A10, annexin A2, and annexin a2 heterotetramer as candidate ... Complexed with the annexin II, p11 binds receptor and channel proteins and guides them to the cell surface, resulting in ... P11 and annexin II form a heterotetrameric protein complex that imitates the structure and function of S-100 proteins activated ...
Board of Directors Annexin Pharmaceuticals. Board of Governors United Nations System Staff College (UNSSC). Members European ... Tundra Fonder, Member of the Board of Directors (since 2017) Annexin Pharmaceuticals, Member of the Board of Directors NGS ...
Von Der Mark, K.; Mollenhauer, J. (1997). "Annexin V interactions with collagen". Cellular and Molecular Life Sciences. 53 (6 ... annexin V (a collagen type II receptor), and integrin receptors (of which there exist several types on chondrocytes). Using the ... and annexin V". Journal of Orthopaedic Research. 19 (6): 1122-30. doi:10.1016/S0736-0266(01)00051-1. PMID 11781014. Pulai, ...
... interleukin 5 and annexin A1). DRESS syndrome: Key elements promoting tissue injury in the DRESS syndrome are: Th2 cells and ...
Thus, anti-annexin A5 antibodies increase phospholipid-dependent coagulation steps.[8] The Lupus anticoagulant antibodies are ... Annexin A5 forms a shield around negatively charged phospholipid molecules, thus reducing their availability for coagulation. ...
... intermediate filaments also with annexin I and annexin II. S100A11 is able to control reorganization of actin and it is ... Seemann J, Weber K, Gerke V (August 1997). "Annexin I targets S100C to early endosomes". FEBS Letters. 413 (1): 185-90. doi: ... "Calcium-dependent binding of S100C to the N-terminal domain of annexin I". The Journal of Biological Chemistry. 271 (2): 719-25 ... the N-terminal part of annexin I". Structure. 8 (2): 175-84. doi:10.1016/s0969-2126(00)00093-9. PMID 10673436. Shankar J, ...
... sulfatide binding to annexin V accelerates coagulation; third, sulfatide and P-selectin interactions expressed on platelets, ...
Annexin II and bleeding in acute promyelocytic leukemia. N Engl J Med 1991; 340: 994-1004. Mclaughlin M, Fuster V. Three ...
annexin II / S100A10, calmodulin TRPV6 calcium-selective TRP channel kidney, intestine 130:1 TRPV5 annexin II / S100A10, ...
Lennon NJ, Kho A, Bacskai BJ, Perlmutter SL, Hyman BT, Brown RH (2003). "Dysferlin interacts with annexins A1 and A2 and ... including annexin and MG53. Exactly how dysferlin contributes to membrane resealing is not clear, but biochemical evidence ... "Enhanced Muscular Dystrophy from Loss of Dysferlin Is Accompanied by Impaired Annexin A6 Translocation after Sarcolemmal ...
The other group, called annexins, bind calcium and phospholipids such as lipocortin. Many other proteins bind calcium, although ...
Morel, Etienne; Gruenberg, Jean (2007-10-31). "The p11/S100A10 Light Chain of Annexin A2 Is Dispensable for Annexin A2 ... Annexin A2 (previously named Annexin II), the vacuolar ATPase, COP coat proteins, the N-ethylmaleimide sensitive factor, the ... Emans, N.; Gorvel, J. P.; Walter, C.; Gerke, V.; Kellner, R.; Griffiths, G.; Gruenberg, J. (1993-03-02). "Annexin II is a major ... Harder, T.; Kellner, R.; Parton, R. G.; Gruenberg, J. (1997). "Specific release of membrane-bound annexin II and cortical ...
... and annexin A2[93] leading to entry of the virions into basal epithelial cells through clathrin-mediated endocytosis and/or ... "The S100A10 subunit of the annexin A2 heterotetramer facilitates L2-mediated human papillomavirus infection". PLoS One. 7 (8 ...
... annexin-1) synthesis. Lipocortin-1 both suppresses phospholipase A2, thereby blocking eicosanoid production, and inhibits ...
Kirshner J, Schumann D, Shively JE (December 2003). "CEACAM1, a cell-cell adhesion molecule, directly associates with annexin ... CEACAM1 has been shown to interact with PTPN11 and Annexin A2. Carcinoembryonic antigen Cluster of differentiation GRCh38: ...
Demonbreun AR, Quattrocelli M, Barefield DY, Allen MV, Swanson KE, McNally EM (June 2016). "An actin-dependent annexin complex ...
Okuse K, Malik-Hall M, Baker MD, Poon WY, Kong H, Chao MV, Wood JN (June 2002). "Annexin II light chain regulates sensory ... annexin II light chain). It has been shown using the yeast-two hybrid screening method that p11 binds to a 28-amino-acid ... "Deletion of annexin 2 light chain p11 in nociceptors causes deficits in somatosensory coding and pain behavior" (PDF). The ...
Annexin A2, a membrane-bound protein, helps regulate vomocytosis and promote the fusing of vesicles to the plasma membranes. In ... annexin A2 deficient cell lines, rates of vomocytosis were decreased. Furthermore, screens of macrophage kinase inhibitors ...
Annexin A5-stained EVs, etc.); or c) descriptions of conditions or cell of origin (podocyte EVs, hypoxic EVs, large oncosomes, ...
Structure and stability of per-thiaproline mutant or annexin V". Proc. Natl. Acad. Sci. USA. 95 (2): 455-459. doi:10.1073/pnas. ...
Crystals can attach to membranes via annexin II, CD44, and osteopontin. The most straightforward treatment of crystallopathies ...
"Activation of the annexin A1 pathway underlies the protective effects exerted by estrogen in polymorphonuclear leukocytes" ...
One candidate is Annexin A2, identified as being able of binding progastrin and derived peptides. Annexin A2 is a partial ... In addition, annexin A2 may be involved in progastrin-dependent clathrin endocytosis. However, progastrin affinity for Annexin ... And, although Annexin A2 plays a role in progastrin functions, it does not fit a receptor function. Another candidate is the G ... In particular, Annexin A2 mediates NF-kappaB upregulation and ß-catenin in response to progastrin in mice and HEK-293 cells. ...
Seidah NG, Poirier S, Denis M, Parker R, Miao B, Mapelli C, Prat A, Wassef H, Davignon J, Hajjar KA, Mayer G (2012). "Annexin ... Annexin A2, an endogenous protein, is a natural inhibitor of PCSK9 activity. GRCh38: Ensembl release 89: ENSG00000169174 - ... Mayer G, Poirier S, Seidah NG (November 2008). "Annexin A2 is a C-terminal PCSK9-binding protein that regulates endogenous low ...
Annexin A1 List of cutaneous conditions "Hairy cell leukemia: MedlinePlus Medical Encyclopedia". medlineplus.gov. Retrieved ...
However some annexins (Annexin A1, Annexin A2, and Annexin A5) have also been found outside the cellular environment, for ... Annexin, type I InterPro: IPR002388 Annexin, type II InterPro: IPR002389 Annexin, type III InterPro: IPR002390 Annexin, type IV ... In addition to annexin A-II, annexin A-XI has also been shown to organize cell membrane properties. Annexin A-XI is believed to ... The 310 amino acid annexin core has four annexin repeats, each composed of 5 alpha-helices. The exception is annexin A-VI that ...
The concept for the therapy is based on the anti-inflammatory properties of Annexin A5, a body own protective protein that acts ... Annexin Pharmaceuticals is a Swedish privately held biotech company developing new therapeutic approaches for inflammatory ... The company is currently focusing on treatment of peripheral artery disease (PAD). Martz, Lauren (May 26, 2014). "Annexin: ... CS1 maint: discouraged parameter (link) http://www.solidinfo.se/foretag/annexin-pharmaceuticals-ab/personer www. ...
In cell fractionation studies, annexin C2 cofractionates with annexin C1 and is enriched in the 100,000 g pellet. Like annexin ... and the annexin C2 gene (nxnB) coding for a 56-kDa protein with 33% sequence identity to annexin C1. Annexin C2 is expressed at ... Annexin C2 is not observed in the nucleus. An annexin C1 mutant (SYN-) which shows a defect during multicellular development ... Analysis of the Dictyostelium genome revealed the presence of two annexin genes, the annexin C1 gene (nxnA) giving rise to two ...
annexin family [Micromonas commoda] annexin family [Micromonas commoda]. gi,255073503,ref,XP_002500426.1, ...
Lähetetään 5‑9 arkipäivässä.. Osta kirja Annexins (ISBN 9781461348412) osoitteesta Adlibris.fi. Ilmainen toimitus Meillä on ... The Annexins is focused on a specific family of calcium and membrane-binding proteins, annexins, ubiquitously spread within ... provides an up-to-date overview of potential physiological roles of annexins. Growing evidence of participation of annexins in ... The Annexin V Binding Properties of Platelet-Derived Microparticles. Connor David. 68,50 € ...
A unique class of these proteins - annexins - can bind to certain membrane phospholipids in a Ca2+-dependent manner, providing ... By forming networks on the membrane surface, annexins can function as organizers of membrane domains and membrane-recruitment ... These and related properties enable annexins to participate in several otherwise unrelated events that range from membrane ... HYDRA ANNEXIN. Annexin B12 is the predominant annexin in the freshwater cnidarian Hydra vulgaris, and has been the prototype ...
IPR001464 Annexin. IPR018502 Annexin_repeat. IPR018252 Annexin_repeat_CS. IPR037104 Annexin_sf. ... IPR001464 Annexin. IPR018502 Annexin_repeat. IPR018252 Annexin_repeat_CS. IPR037104 Annexin_sf. ... A pair of annexin repeats may form one binding site for calcium and phospholipid.UniRule annotation. Automatic assertion ... Belongs to the annexin family.UniRule annotation. Automatic assertion according to rulesi ...
Annexin V,PAP-1; find Sigma-Aldrich-A9210 MSDS, related peer-reviewed papers, technical documents, similar products & more at ... Annexin V FITC Conjugate from human placenta; Synonyms: Calphosbindin I,Lipocortin V, ... Annexin V conjugates can be used to detect apoptotic cells significantly earlier than DNA-based assays. Fluorescent dye labeled ... Annexins are ubiquitous homologous proteins that bind phospholipids in the presence of calcium. The cellular changes involved ...
... Thomas M. Raffay,1 Morgan L. Locy,2 Cynthia ... Expression of lung SCGB and annexin A1 (ANXA1) is persistently altered in CCSP knockout mice suggesting that CCSP indirectly ...
IPR001464. Annexin. IPR018502. Annexin_repeat. IPR018252. Annexin_repeat_CS. IPR037104. Annexin_sf. IPR002388. ANX1. ... IPR001464. Annexin. IPR018502. Annexin_repeat. IPR018252. Annexin_repeat_CS. IPR037104. Annexin_sf. IPR002388. ANX1. ... Annexin 1Add BLAST. 61. ,p>This subsection of the Family and Domains section indicates the positions and types of repeated ... Annexin 2Add BLAST. 61. ,p>This subsection of the Family and Domains section indicates the positions and types of repeated ...
Goat polyclonal Annexin A2 antibody validated for WB and tested in Human. Referenced in 2 publications and 1 independent review ... Anti-Annexin A2 antibody (ab2242) at 0.5 µg/ml + A431 lysate in RIPA buffer at 35 µg. Developed using the ECL technique.. ... Detects a band of approximately 35 kDa (predicted molecular weight: 38 kDa).Can be blocked with Human Annexin A2 peptide ( ... A pair of annexin repeats may form one binding site for calcium and phospholipid. ...
Rabbit polyclonal Annexin A1 antibody validated for WB, IHC, ICC/IF and tested in Human. Immunogen corresponding to recombinant ... Anti-Annexin A1 antibody (ab97485) at 1/1000 dilution + H1299 whole cell lysate at 30 µg. Predicted band size : 39 kDa. ... A pair of annexin repeats may form one binding site for calcium and phospholipid. ... Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1-198 of Human Annexin A1 ( ...
Compare Anti-Annexin A5 Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, reviews ... Anti-Annexin A5 Antibody Products. Anti-Annexin A5 antibodies are available from several suppliers. In humans, this protein is ... Annexin V is an early apoptotic marker and is often used for detecting apoptosis in cells. Annexins are a family of calcium- ... We typically use Annexin V in combination with 7AAD or PI, to determine apoptosis by flow cytometry. We prefer the... Read ...
... H. S. Euzger,1 R. J. Flower,2 N. J. Goulding,1 ... H. S. Euzger, R. J. Flower, N. J. Goulding, and M. Perretti, "Differential Modulation of Annexin I Binding Sites on Monocytes ...
Michael Davidsons lab contains the insert Annexin. This plasmid is available through Addgene. ... mEmerald-Annexin-12 was a gift from Michael Davidson (Addgene plasmid # 53991 ; http://n2t.net/addgene:53991 ; RRID:Addgene_ ...
... português (Portugal). dansk (Danmark). čeština (Česká republika). ...
To elucidate how annexins facilitate repair, we visualized annexin cap formation during injury. We found that annexin cap size ... These data identify annexins as mediators of membrane-associated Ca2+ release during membrane repair and annexin A6 as a ... Annexins are a family of membrane-associated Ca2+-binding proteins implicated in repair, and annexin A6 was previously ... Annexin A6 overexpression reduced membrane injury, consistent with enhanced repair. Treatment with recombinant annexin A6 ...
In contrast, annexin A6 contains 2 annexin cores and thus 8 annexin-repeat domains (22). Annexin A6s duplicated structure ... annexin A1 (NM_010730), annexin A2 (NM_007585), annexin A6 (NM_013472), and annexin A6-encoding sequencing from multiple ... To elucidate how annexins facilitate repair, we visualized annexin cap formation during injury. We found that annexin cap size ... Annexins have the ability to self- and hetero-oligomerize (21). Typical annexins like A1 and A2 contain 1 annexin core composed ...
Annexins V and VI: major calcium-dependent atrial secretory granule-binding proteins.. A F Doubell, A J Bester, G Thibault ... The two major proteins isolated (32.5 kd and 67 kd) are calcium-binding proteins and have been identified as annexins V and VI ... Annexins V and VI: major calcium-dependent atrial secretory granule-binding proteins. ... Annexins V and VI: major calcium-dependent atrial secretory granule-binding proteins. ...
Annexin A-V has a major role in coagulation. Annexin AII has a major role in fibrinolysis. Annexins bind phospholipids and Ca+2 ... Annexins divide into species and are numbered from I to XII. Annexin V is the most abundant scaffolding protein. Annexin E1 ( ... Annexin I is involved in anti-inflammatory responses and apoptotic mechanisms. Structural highlights Annexins consist of 2 ... Annexins are proteins which make a membrane scaffold. They bind negatively charged phospholipids and contain a 70 amino acid ...
Shop a large selection of Apoptosis Reagents and Kits products and learn more about BD Annexin V-FITC:Life Sciences:Cell ...
Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in ... Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in ... Annexin V Binding Buffer (Cat. No. 422201) is recommended for use with Annexin V staining. Annexin V binding alone cannot ... Why do I need to use Annexin V Binding Buffer? Annexin V binding requires the presence of calcium in the solution. So, we ...
... annexin A1), Authors: Flavia Cristina Rodrigues-Lisoni, Tiago Henrique, Eloiza Helena Tajara. Published in: Atlas Genet ... Similarly to other annexins, annexin A1 is characterized by a C-terminal homologous domain with 4 to 8 repeats of 70-75 ... The comparison of conserved and variable residues between human annexin 1 protein and annexin protein from different ... Loss of annexin A1 expression in breast cancer progression.. Cao Y, Li Y, Edelweiss M, Arun B, Rosen D, Resetkova E, Wu Y, Liu ...
mKate2-annexin fusion: 603-2313. mKate2: 603-1314. Start codon (ATG): 603-605. Last amino acid in mKate2: 1312-1314. Annexin A4 ... pmKate2-annexin. FP321. 20 μg. € 400 / 200*. *50% discount on the second and subsequent vectors encoding same fluorescent ... pmKate2-annexin vector. cat.# FP321. The vector sequence has been compiled using the information from sequence databases, ...
Mutational and crystallographic analyses of interfacial residues in annexin V suggest direct interactions with phospholipid ... Description: ANNEXIN V protein , Length: 319 No structure alignment results are available for 1BCW.A explicitly.. It is ...
Mutational and crystallographic analyses of interfacial residues in annexin V suggest direct interactions with phospholipid ... ANNEXIN V protein, length: 319 (BLAST) Sequence Similarity Cutoff. Rank. Chains in Cluster. Cluster ID / Name. Structural ...
Browse our Annexin 2 Receptor RNAi catalog backed by our Guarantee+. ... Alternate Names for Annexin 2 Receptor RNAi. Annexin 2 Receptor RNAi, ANXA2R RNAi, Annexin II receptor RNAi, annexin-2 receptor ... Annexin 2 Receptor RNAi. We offer Annexin 2 Receptor RNAi for use in common research applications. Each Annexin 2 Receptor RNAi ... Our Annexin 2 Receptor RNAi can be used in a variety of model species: Human. Use the list below to choose the Annexin 2 ...
Browse our Annexin V Antibody Pair catalog backed by our Guarantee+. ... Annexin V Antibody Pairs available through Novus Biologicals. ... Annexin V Antibody Pairs. We offer Annexin V Antibody Pairs for ... Alternate Names for Annexin V Antibody Pairs. Annexin V antibody pair, ANXA5 antibody pair, Anchorin CII antibody pair, annexin ... Our Annexin V Antibody Pairs can be used in a variety of model species: Human. Use the list below to choose the Annexin V ...
ANXA3 (annexin A3). NCBI. Ortholog. Mus musculus (house mouse):. Anxa3 (annexin A3). Transitive Ortholog Pipeline. Transitive ... Anxa3 (annexin A3). Transitive Ortholog Pipeline. Transitive Ortholog Pipeline. Sus scrofa (pig):. ANXA3 (annexin A3). ... ANXA3 (annexin A3). Transitive Ortholog Pipeline. Transitive Ortholog Pipeline. Canis lupus familiaris (dog):. ANXA3 (annexin ... Homo sapiens (human) : ANXA3 (annexin A3) HGNC Alliance Mus musculus (house mouse) : Anxa3 (annexin A3) MGI Alliance Rattus ...
Serum antibodies against annexin A2 were significantly elevated in subjects with proliferative LN, validating those antibodies ... annexin A2. By ELISA, patients with proliferative LN demonstrated significantly increased antibodies against annexin A2. ... Autoantibodies targeting glomerular annexin A2 identify patients with proliferative lupus nephritis Proteomics Clin Appl. 2015 ... Serum antibodies against annexin A2 were significantly elevated in subjects with proliferative LN, validating those antibodies ...

No FAQ available that match "annexins"