Annexin A2: A member of the annexin family that is a substrate for a tyrosine kinase, ONCOGENE PROTEIN PP60(V-SRC). Annexin A2 occurs as a 36-KDa monomer and in a 90-KDa complex containing two subunits of annexin A2 and two subunits of S100 FAMILY PROTEIN P11. The monomeric form of annexin A2 was formerly referred to as calpactin I heavy chain.Annexin A1: Protein of the annexin family exhibiting lipid interaction and steroid-inducibility.Annexin A5: A protein of the annexin family isolated from human PLACENTA and other tissues. It inhibits cytosolic PHOSPHOLIPASE A2, and displays anticoagulant activity.Annexin A6: Protein of the annexin family with a probable role in exocytotic and endocytotic membrane events.Annexin A4: Protein of the annexin family originally isolated from the electric organ of the electric ray Torpedo marmorata. It has been found in a wide range of mammalian tissue where it is localized to the apical membrane of polarized EPITHELIAL CELLS.Annexin A7: An annexin family member that plays a role in MEMBRANE FUSION and signaling via VOLTAGE-DEPENDENT CALCIUM CHANNELS.Annexin A3: A protein of the annexin family that catalyzes the conversion of 1-D-inositol 1,2-cyclic phosphate and water to 1-D-myo-inositol 1-phosphate.Annexins: Family of calcium- and phospholipid-binding proteins which are structurally related and exhibit immunological cross-reactivity. Each member contains four homologous 70-kDa repeats. The annexins are differentially distributed in vertebrate tissues (and lower eukaryotes) and appear to be involved in MEMBRANE FUSION and SIGNAL TRANSDUCTION.S100 Proteins: A family of highly acidic calcium-binding proteins found in large concentration in the brain and believed to be glial in origin. They are also found in other organs in the body. They have in common the EF-hand motif (EF HAND MOTIFS) found on a number of calcium binding proteins. The name of this family derives from the property of being soluble in a 100% saturated ammonium sulfate solution.Phosphatidylserines: Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a serine moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and serine and 2 moles of fatty acids.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.Propidium: Quaternary ammonium analog of ethidium; an intercalating dye with a specific affinity to certain forms of DNA and, used as diiodide, to separate them in density gradients; also forms fluorescent complexes with cholinesterase which it inhibits.Calcium: A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.Phospholipids: Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.Calcium-Binding Proteins: Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Cell Line, Tumor: A cell line derived from cultured tumor cells.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Flow Cytometry: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Cell-Derived Microparticles: Extracellular vesicles generated by the shedding of CELL MEMBRANE blebs.Receptors, Formyl Peptide: A family of G-protein-coupled receptors that was originally identified by its ability to bind N-formyl peptides such as N-FORMYLMETHIONINE LEUCYL-PHENYLALANINE. Since N-formyl peptides are found in MITOCHONDRIA and BACTERIA, this class of receptors is believed to play a role in mediating cellular responses to cellular damage and bacterial invasion. However, non-formylated peptide ligands have also been found for this receptor class.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Caspase 3: A short pro-domain caspase that plays an effector role in APOPTOSIS. It is activated by INITIATOR CASPASES such as CASPASE 9. Isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.Organotechnetium Compounds: Organic compounds that contain technetium as an integral part of the molecule. These compounds are often used as radionuclide imaging agents.Cell Survival: The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Caspases: A family of intracellular CYSTEINE ENDOPEPTIDASES that play a role in regulating INFLAMMATION and APOPTOSIS. They specifically cleave peptides at a CYSTEINE amino acid that follows an ASPARTIC ACID residue. Caspases are activated by proteolytic cleavage of a precursor form to yield large and small subunits that form the enzyme. Since the cleavage site within precursors matches the specificity of caspases, sequential activation of precursors by activated caspases can occur.Fluorescein-5-isothiocyanate: Fluorescent probe capable of being conjugated to tissue and proteins. It is used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.In Situ Nick-End Labeling: An in situ method for detecting areas of DNA which are nicked during APOPTOSIS. Terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 prime OH ends of either single- or double-stranded DNA. The terminal deoxynucleotidyl transferase nick end labeling, or TUNEL, assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA FRAGMENTATION.Fibrinolysin: A product of the lysis of plasminogen (profibrinolysin) by PLASMINOGEN activators. It is composed of two polypeptide chains, light (B) and heavy (A), with a molecular weight of 75,000. It is the major proteolytic enzyme involved in blood clot retraction or the lysis of fibrin and quickly inactivated by antiplasmins.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.

HIV-1 Gag shares a signature motif with annexin (Anx7), which is required for virus replication. (1/67)

Genetic and biochemical analyses of the Gag protein of HIV-1 indicate a crucial role for this protein in several functions related to viral replication, including viral assembly. It has been suggested that Gag may fulfill some of the functions by recruiting host cellular protein(s). In our effort to identify structural and functional homologies between Gag and cellular cytoskeletal and secretory proteins involved in transport, we observed that HIV-1 Gag contains a unique PGQM motif in the capsid region. This motif was initially noted in the regulatory domain of synexin the membrane fusion protein of Xenopus laevis. To evaluate the functional significance of the highly conserved PGQM motif, we introduced alanine (A) in place of individual residues of the PGQM and deleted the motif altogether in a Gag expression plasmid and in an HIV-1 proviral DNA. The proviral DNA containing mutations in the PGQM motif showed altered expression, assembly, and release of viral particles in comparison to parental (NL4-3) DNA. When tested in multiple- and single-round replication assays, the mutant viruses exhibited distinct replication phenotypes; the viruses containing the A for the G and Q residues failed to replicate, whereas A in place of the P and M residues did not inhibit viral replication. Deletion of the tetrapeptide also resulted in the inhibition of replication. These results suggest that the PGQM motif may play an important role in the infection process of HIV-1 by facilitating protein-protein interactions between viral and/or viral and cellular proteins.  (+info)

Annexin VII and annexin XI are tyrosine phosphorylated in peroxovanadate-treated dogs and in platelet-derived growth factor-treated rat vascular smooth muscle cells. (2/67)

The intraperitoneal administration of peroxovanadate results in the rapid accumulation of many tyrosine-phosphorylated proteins in the liver and kidney of treated animals. The availability of large pools of tyrosine-phosphorylated proteins derived from normal tissues facilitates the purification and identification of previously unknown targets for cellular tyrosine kinases. Using this procedure, we have thus far identified four proteins in the liver and kidney of peroxovanadate-treated dogs. Two of these, annexin VII and annexin XI, were novel and had not been previously reported to be substrates of tyrosine kinases while the remaining two, ezrin and clathrin, have been reported to be tyrosine phosphorylated in some cell culture systems. In the present study, isolated proteins were identified both by sequence analysis and immunological methods. Annexin VII and annexin XI are present in cultured rat vascular smooth muscle cells and both were tyrosine phosphorylated in response to a physiological ligand, platelet-derived growth factor-BB (PDGF-BB). Furthermore, the extent of tyrosine phosphorylation in response to PDGF-BB was augmented by the co-addition of peroxovanadate to cell cultures. In vitro phosphorylation assays showed that PDGF receptor, calcium-dependent tyrosine kinase (CADTK/Pyk-2), Src kinase, and epidermal growth factor receptor all were able to phosphorylate purified annexin VII and XI on tyrosine residues. These findings confirm the usefulness of phosphatase inhibition by peroxovanadate as a tool for identifying previously unknown physiological targets for cellular protein tyrosine kinases.  (+info)

Defects in inositol 1,4,5-trisphosphate receptor expression, Ca(2+) signaling, and insulin secretion in the anx7(+/-) knockout mouse. (3/67)

The mammalian anx7 gene codes for a Ca(2+)-activated GTPase, which supports Ca(2+)/GTP-dependent secretion events and Ca(2+) channel activities in vitro and in vivo. To test whether anx7 might be involved in Ca(2+) signaling in secreting pancreatic beta cells, we knocked out the anx7 gene in the mouse and tested the insulin-secretory properties of the beta cells. The nullizygous anx7 (-/-) phenotype is lethal at embryonic day 10 because of cerebral hemorrhage. However, the heterozygous anx7 (+/-) mouse, although expressing only low levels of ANX7 protein, is viable and fertile. The anx7 (+/-) phenotype is associated with a substantial defect in insulin secretion, although the insulin content of the islets, is 8- to 10-fold higher in the mutants than in the normal littermate control. We infer from electrophysiological studies that both glucose-stimulated secretion and voltage-dependent Ca(2+) channel functions are normal. However, electrooptical recordings indicate that the (+/-) mutation has caused a change in the ability of inositol 1,4,5-trisphosphate (IP(3))-generating agonists to release intracellular calcium. The principle molecular consequence of lower anx7 expression is a profound reduction in IP(3) receptor expression and function in pancreatic islets. The profound increase in islets, beta cell number, and size may be a means of compensating for less efficient insulin secretion by individual defective pancreatic beta cells. This is a direct demonstration of a connection between glucose-activated insulin secretion and Ca(2+) signaling through IP(3)-sensitive Ca(2+) stores.  (+info)

Annexins VII and XI are present in a human macrophage-like cell line. Differential translocation on FcR-mediated phagocytosis. (4/67)

We have studied the divalent cation-dependent association of proteins to subcellular fractions of human macrophage-like cells before and after FcR-mediated phagocytosis. Among these proteins we have identified annexins VII and XI for the first time in these cells, along with annexins I, III, and VI. Although all of these annexins are present in the cytosolic fraction, the extent of their association to membrane and phagosome fractions from resting and stimulated cells is variable. Annexin VII translocates from cytosolic to membrane fractions after phagocytic stimulation, along with annexin I, III, and VI. Annexins VII and XI are found associated with purified phagosomes along with I, III, and VI, and this association is greater after a 24-h chase period. Our results show differences in the intracellular distribution of different annexins in macrophage-like cells on phagocytosis. Annexins VII, VI, III, and I respond to particle ingestion by translocating to phagosomes and other cell membrane structures, whereas annexin XI translocates predominantly to phagosomes, suggesting dissimilarities in their function.  (+info)

The sorcin-annexin VII calcium-dependent interaction requires the sorcin N-terminal domain. (5/67)

Surface plasmon resonance experiments show that at neutral pH the stability of the complex between sorcin and annexin VII (synexin) increases dramatically between 3 and 6 microM calcium; at the latter cation concentration the K(D) value is 0.63 microM. In turn, the lack of complex formation between the sorcin Ca(2+) binding domain (33-198) and synexin maps the annexin binding site to the N-terminal region of the sorcin polypeptide chain. Annexin VII likewise employs the N-terminal domain, more specifically the first 31 amino acids, to interact with sorcin [Brownawell, A.M. and Creutz, C.E. (1997) J. Biol. Chem. 272, 22182-22190]. The interaction may involve similar structural motifs in the two proteins, namely GGYY and GYGG in sorcin and GYPP in synexin.  (+info)

The annexinopathies: a new category of diseases. (6/67)

The annexins are a family of highly homologous phospholipid binding proteins, which share a four-domain structure, with one member of the family - annexin VI - having a duplication consisting of eight domains. Thus far, ten annexins have been described in mammals. Although the biological functions of the annexins have not been definitively established, two human diseases involving annexin abnormalities ('annexinopathies') have been identified as of the time of writing. Overexpression of annexin II occurs in the leukocytes of a subset of patients having a hemorrhagic form of acute promyelocytic leukemia. Underexpression of annexin V occurs on placental trophoblasts in the antiphospholipid syndrome and in preeclampsia. Also, an animal model has been described in which annexin VII is underexpressed and is associated with disease, but the relevance of this animal model to human disease is not yet understood. Future research is likely to elucidate additional 'annexinopathies'.  (+info)

Activation of annexin 7 by protein kinase C in vitro and in vivo. (7/67)

Annexin 7, a Ca(2+)/GTP-activated membrane fusion protein, is preferentially phosphorylated in intact chromaffin cells, and the levels of annexin 7 phosphorylation increase quantitatively in proportion to the extent of catecholamine secretion. Consistently, various protein kinase C inhibitors proportionately reduce both secretion and phosphorylation of annexin 7 in these cells. In vitro, annexin 7 is quantitatively phosphorylated by protein kinase C to a mole ratio of 2.0, and phosphorylation is extraordinarily sensitive to variables such as pH, calcium, phospholipid, phorbol ester, and annexin 7 concentration. Phosphorylation of annexin 7 by protein kinase C significantly potentiates the ability of the protein to fuse phospholipid vesicles and lowers the half-maximal concentration of calcium needed for this fusion process. Furthermore, other protein kinases, including cAMP-dependent protein kinase, cGMP-dependent protein kinase, and protein-tyrosine kinase pp60(c-)(src), also label annexin 7 with high efficiency but do not have this effect on membrane fusion. In the case of pp60(c-)(src), we note that this kinase, if anything, modestly suppresses the membrane fusion activity of annexin 7. These results thus lead us to hypothesize that annexin 7 may be a positive mediator for protein kinase C action in the exocytotic membrane fusion reaction in chromaffin cells.  (+info)

ANX7, a candidate tumor suppressor gene for prostate cancer. (8/67)

The ANX7 gene is located on human chromosome 10q21, a site long hypothesized to harbor a tumor suppressor gene(s) (TSG) associated with prostate and other cancers. To test whether ANX7 might be a candidate TSG, we examined the ANX7-dependent suppression of human tumor cell growth, stage-specific ANX7 expression in 301 prostate specimens on a prostate tissue microarray, and loss of heterozygosity (LOH) of microsatellite markers at or near the ANX7 locus. Here we report that human tumor cell proliferation and colony formation are markedly reduced when the wild-type ANX7 gene is transfected into two prostate tumor cell lines, LNCaP and DU145. Consistently, analysis of ANX7 protein expression in human prostate tumor microarrays reveals a significantly higher rate of loss of ANX7 expression in metastatic and local recurrences of hormone refractory prostate cancer as compared with primary tumors (P = 0.0001). Using four microsatellite markers at or near the ANX7 locus, and laser capture microdissected tumor cells, 35% of the 20 primary prostate tumors show LOH. The microsatellite marker closest to the ANX7 locus showed the highest rate of LOH, including one homozygous deletion. We conclude that the ANX7 gene exhibits many biological and genetic properties expected of a TSG and may play a role in prostate cancer progression.  (+info)

*Annexin A7

... is a protein that in humans is encoded by the ANXA7 gene. Annexin VII is a member of the annexin family of calcium- ... "Entrez Gene: ANXA7 annexin A7". Satoh, Hirokazu; Nakano Yoshimi; Shibata Hideki; Maki Masatoshi (Nov 2002). "The penta-EF-hand ... "The penta-EF-hand domain of ALG-2 interacts with amino-terminal domains of both annexin VII and annexin XI in a Ca2+-dependent ... The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in ...

*List of MeSH codes (D12.776.157)

... annexin a5 MeSH D12.776.157.125.050.110 -- annexin a6 MeSH D12.776.157.125.050.120 -- annexin a7 MeSH D12.776.157.125.412.249 ... annexin a1 MeSH D12.776.157.125.050.060 -- annexin a2 MeSH D12.776.157.125.050.070 -- Annexin A3 MeSH D12.776.157.125.050.080 ...
Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. The alternative splicing event is tissue specific and the mRNA containing the cassette exon is prevalent in brain, heart and skeletal muscle. The transcripts also differ in their 3-non coding regions by the use of two alternative poly(A) signals. Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, ...
Calcium flux is required for the mammalian sperm acrosome reaction, an exocytotic event triggered by egg binding, which results in a dramatic rise in sperm intracellular calcium. Calcium-dependent membrane fusion results in the release of enzymes that facilitate sperm penetration through the zona pellucida during fertilization. We have characterized inositol 1,4,5-trisphosphate (IP3)-gated calcium channels and upstream components of the phosphoinositide signaling system in mammalian sperm. Peptide antibodies colocalized G alpha q/11 and the beta 1 isoform of phospholipase C (PLC beta 1) to the anterior acrosomal region of mouse sperm. Western blotting using a polyclonal antibody directed against purified brain IP3 receptor (IP3R) identified a specific 260 kD band in 1% Triton X-100 extracts of rat, hamster, mouse and dog sperm. In each species, IP3R immunostaining localized to the acrosome cap. Scatchard analysis of [3H]IP3 binding to rat sperm sonicates revealed a curvilinear plot with high ...
Rabbit polyclonal Annexin VII antibody validated for WB, IHC and tested in Human. With 1 independent review. Immunogen corresponding to recombinant fragment
SCAN HISTORY OWL11_0 2 100 NSINGLE OWL17_1 2 110 NSINGLE OWL18_0 1 115 NSINGLE OWL19_1 1 125 NSINGLE OWL21_1 1 125 NSINGLE OWL26_0 1 125 NSINGLE SPTR37_9f 2 237 NSINGLE INITIAL MOTIF SETS ANNEXIN1 Length of motif = 23 Motif number = 1 Annexin motif I - 1 PCODE ST INT KTKGVDEVTIVNILTNRSNAQRQ LUHU36 46 46 KTKGVDEVTIINILTNRSNEQRQ ANX2_CHICK 46 46 TVKGVDEATIIDILTKRNNAQRQ ANX1_CAVCU 56 56 MVKGVDEATIIDILTKRNNAQRQ LUHU 55 55 KGIGTDEATIIDIVTHRSNAQRQ ANX6_MOUSE 376 376 KGMGTDEETILKILTSRNNAQRQ ANX5_CHICK 29 29 KGIGTNEQAIIDVLTKRSNTQRQ ANX8_HUMAN 35 35 KGFGTDEQEIIDVLVGRSNQQRQ DROANNX 29 29 RGIGTDEKMLISILTERSNAQRQ ANX3_HUMAN 32 32 KGLGTDEDAIINVLAYRSTAQRQ ANX4_BOVIN 27 27 KGFGTDEQAIVDVVANRSNDQRQ HUMSNEXIN 177 177 TAKGVDEATIIDIMTTRTNAQRP ANX1_COLLI 51 51 ANNEXIN2 Length of motif = 17 Motif number = 2 Annexin motif II - 1 PCODE ST INT LKSALSGHLETVILGLL LUHU36 86 17 LKSALSGHLEAVILGLL ANX2_CHICK 86 17 LKKALTGHLEEVVLALL ANX1_CAVCU 96 17 LKKALTGHLEEVVLALL LUHU 95 17 LKSEISGDLARLILGLM ANX6_MOUSE 416 17 ...
1. If E, F are fields and F is a subring of E, show each q in Aut(E/F) permutes the roots in E of each nonzero p(x) in F[x]. Hint if p(x)=a0+a1x+a2x^2+. . . +anx^n then p(x) has at most n roots in E. show that for z in E,.
Cancer Research UK is a registered charity in England and Wales (1089464), Scotland (SC041666) and the Isle of Man (1103). A company limited by guarantee. Registered company in England and Wales (4325234) and the Isle of Man (5713F). Registered address: Angel Building, 407 St John Street, London EC1V 4AD ...
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis. Alternatively spliced transcript variants have been described. [provided by RefSeq, Aug 2010 ...
Copines make up a family of soluble, calcium-dependent membrane binding proteins found in a wide variety of eukaryotic organisms. Copines are characterized as having two C2 domains at the N-terminal region followed by an "A domain," similar to the von Willebrand A (VWA) domain found in integrins, in the C-terminal region [1]. Copines appear to be absent from the Sacchromyces cerevisae genome, while the genomes of Paramecium, Arabidopsis, C. elegans, and human have two, three, five, and nine, respectively [13, 4, 24]. Tomsig and Creutz [8] have hypothesized that copines play a role in calcium signaling by binding to target proteins with their A domains and then bringing those target proteins to a particular membrane through the action of their C2 domains in response to a rise in calcium concentration. Because copines have two C2 domains, they have also been hypothesized to have a role in membrane trafficking. It is possible that both ideas could be correct; copines may provide links between ...
The anorectic anx/anx mouse exhibits disturbed feeding behavior and aberrances, including neurodegeneration, in peptidergic neurons in the appetite regulating hypothalamic arcuate nucleus. Poor feeding in infants, as well as neurodegeneration, are common phenotypes in human disorders caused by dysfunction of the mitochondrial oxidative phosphorylation system (OXPHOS). We therefore hypothesized that the anorexia and degenerative phenotypes in the anx/anx mouse could be related to defects in the OXPHOS. In this study, we found reduced efficiency of hypothalamic OXPHOS complex I assembly and activity in the anx/anx mouse. We also recorded signs of increased oxidative stress in anx/anx hypothalamus, possibly as an effect of the decreased hypothalamic levels of fully assembled complex I, that were demonstrated by native Western blots. Furthermore, the Ndufaf1 gene, encoding a complex I assembly factor, was genetically mapped to the anx interval and found to be down-regulated in anx/anx mice. These ...
Hi guys , Ive been trying for a month now with the help of Pregnitude. Has anyone else used this product? Ive seen results already. Went through my first full period cycle Im a long time . A bit anx...
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Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 has 45 to 59% identity with other members of its family and shares a similar size and exon-intron organization. Isolated from human placenta, ANX4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells ...
Annexin A5 is a biomarker used in Hiv Infection, Acute Lymphoblastic Leukemia, Hypertension and 942 other diseases. Learn more about Annexin A5.
Annexin A1 Antibody 66344-1-Ig has been identified with ELISA, IF, IHC, WB. 66344-1-Ig detected 35 kDa band in A431 cells with 1:2500-1:10000 dilution...
ADVENTRX Pharmaceuticals, Inc. (NYSE Amex ANX) today announced that it has completed the previously announced sale of shares of convertible preferred stock purs
In our hands, the trypsin actually reduced the Annexin staining presumably by stripping off all the phosphotidyl-serine on the surface along with the surface proteins (adhesion molecules). The only time we have seen enhanced annexin is when the cells suffer enough trauma in harvesting that the membrane is not intact and the Annexin gets inside the cell and attaches to the ps on the inner membrane. Julie At 02:11 PM 8/18/98, you wrote: , ,I have a client who is staining with Annexin-FITC. They are looking at ,cell lines and are using trypsin to get the cells off the flasks. We are ,seeing what we think is an exagerated Annexin response. Does anyone out ,there have any feedback on how trypsin affects Annexin staining? Any ,experience and or information about this will be greatly appreciated. , Thanks in advance, , Joan Kalnitsky ,Joan Kalnitsky ,Flow Cytometry Laboratory ,VMRCVM ,(540) 231-4115 ,FAX 540-231-7367 , , It is better to serve than to receive , B. Borg , , , , , ...
|p|Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry
BioAssay record AID 590043 submitted by ChEMBL: Induction of apoptosis in human K562 cells assessed as viable cells at 50 nM using annexin V-propidium iodide and annexin V staining by flow cytometry (Rvb = 87.02%).
Annexin A11 Antibody 10479-2-AP has been identified with IF, IHC, IP, WB, ELISA. 10479-2-AP detected 56-60 kDa band in HeLa cells with 1:500-1:2000 dilution...
Bachem offers H-3196 Annexin A1 (1-25) (dephosphorylated) (human) for your research. Find all specific details here. Find product specific information including available pack sizes, CAS, detailed description and references here.
The anx/anx mouse displays poor appetite and lean appearance and is considered a good model for the study of anorexia nervosa. To identify new genes involved in feeding behavior and body weight regulation we performed an expression profiling in the hypothalamus of the anx/anx mice. Using commercial microarrays we detected 156 differentially expressed genes and validated 92 of those using TaqMan low-density arrays. The expression of a set of 87 candidate genes selected based on literature evidences was also quantified by TaqMan low-density arrays. Our results showed enrichment in deregulated genes involved in cell death, cell morphology and cancer as well as an alteration of several signaling circuits involved in energy balance including neuropeptide Y and melanocortin signaling. The expression profile along with the phenotype led us to conclude that anx/anx mice resemble the anorexia-cachexia syndrome typically observed in cancer, infection with human immunodeficiency virus or chronic diseases, ...
BioAssay record AID 502910 submitted by ChEMBL: Induction of annexin 2-mediated cytoskeleton aggregate accumulation in human HepG2 cells at 4 uM after 2 hrs by confocal laser scanning microscopy.
Page contains details about annexin V conjugated to maleimide-functionalized Feraheme nanoparticles . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
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Annexin A2 is a calcium regulated protein involved in membrane binding and actin polymerization. In this paper, Kevin Moreau and colleagues identified Annexin A2 as a…. ...
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Lung lamellar bodies, the storage organelles for lung surfactant phosphatidylcholine (PC), maintain an acidic pH that can be increased with weak bases. This study investigates the effect of a weak base, methylamine, on the pH in lamellar bodies and on the trafficking and packaging of newly synthesized PC in lamellar bodies. Methylamine increased the pH of isolated lung lamellar bodies and of lamellar bodies in intact cells. Metabolic labelling of isolated type II cells with [methyl-3H]choline showed that although methylamine (2.5-10 mM) did not alter the labelling of cellular or microsomal PC and disaturated PC, it decreased the labelling of the PC and disaturated PC in lamellar bodies. The packaging of PC in lamellar bodies (the specific activities ratio between the PC in lamellar bodies and the microsomal PC) also decreased in a time- and concentration-dependent manner. The cellular synthesis of PC or its packaging into lamellar bodies was unaltered by brefeldin A, suggesting that the Golgi ...
The Annexin family of calcium-binding proteins is composed of al least thirteen mammalian genes (Annexin A1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. The Annexin family
Annexin A1 / ANXA1, 0.1 ml. Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane.
Buy our Recombinant Human Annexin A13 protein. Ab105616 is a full length protein produced in Escherichia coli and has been validated in SDS-PAGE, MS. Abcam…
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ANXA7 antibodies | AntibodypediaANXA7 antibodies | Antibodypedia

annexin A7. Protein family. Cancer-related genes, Mapped to neXtProt, Mapped to UniProt SWISS-PROT, Predicted intracellular ... Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains ... Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal ... Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, including ...
more infohttps://www.antibodypedia.com/gene/3809/ANXA7

Calcium- and proton-dependent relocation of annexin A6 in Jurkat T cells stimulated for interleukin-2 secretion - Acta...Calcium- and proton-dependent relocation of annexin A6 in Jurkat T cells stimulated for interleukin-2 secretion - Acta...

Chander A, Sen N, Naidu DG, Spitzer AR (2003) Calcium ionophore and phorbol ester increase membrane binding of annexin A7 in ... GTP-induced membrane binding and ion channel activity of annexin VI: is annexin VI a GTP biosensor? Biophys J 82: 2737-2745. ... Annexin A6 (AnxA6) is a Ca2+-dependent membrane-binding protein involved in vesicular traffic. The likely participation of ... Golczak M, Kicinska A, Bandorowicz-Pikula J, Buchet R, Szewczyk A, Pikula S (2001a) Acidic pH-induced folding of annexin VI is ...
more infohttp://psjd.icm.edu.pl/psjd/element/bwmeta1.element.bwnjournal-article-abpv54p261kz

Annexin A7 - WikipediaAnnexin A7 - Wikipedia

Annexin A7 is a protein that in humans is encoded by the ANXA7 gene. Annexin VII is a member of the annexin family of calcium- ... "Entrez Gene: ANXA7 annexin A7". Satoh, Hirokazu; Nakano Yoshimi; Shibata Hideki; Maki Masatoshi (Nov 2002). "The penta-EF-hand ... "The penta-EF-hand domain of ALG-2 interacts with amino-terminal domains of both annexin VII and annexin XI in a Ca2+-dependent ... The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in ...
more infohttps://en.wikipedia.org/wiki/Annexin_A7

Annexin A7 Recombinant ProteinAnnexin A7 Recombinant Protein

... ANXA7 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 150mM NaCl, 1mM DTT ... Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins. Annexin VII has molecular ... Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties including ...
more infohttps://www.neuromics.com/PR27216

Annexin A7 deficiency potentiates cardiac NFAT activity promoting hypertrophic signaling. | Sigma-AldrichAnnexin A7 deficiency potentiates cardiac NFAT activity promoting hypertrophic signaling. | Sigma-Aldrich

Annexin A7 (Anxa7) is a cytoskeletal protein interacting with Ca(2+) signaling which in turn is a crucial factor for cardiac ... Annexin A7 deficiency potentiates cardiac NFAT activity promoting hypertrophic signaling.. [Jakob Voelkl, Ioana Alesutan, ... These observations point to an important role of annexin A7 in the regulation of cardiac NFAT activity and hypertrophic ...
more infohttps://www.sigmaaldrich.com/catalog/papers/24508799

Recombinant Human Annexin A7, T7-tagged ANXA7-68H - Creative BioMartRecombinant Human Annexin A7, T7-tagged ANXA7-68H - Creative BioMart

Recombinant Human annexin A7 produced inE.coliis a non-glycosylated, polypeptide chain containing amino acids (1-466) and ... ANXA7 annexin A7 [ Homo sapiens ]. Synonyms:. ANXA7; annexin A7; SNX; ANX7; SYNEXIN; Annexin A7; Annexin-7; Annexin VII; ... Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins. Annexin VII has molecular ... Recombinant Human Annexin A7, T7-tagged. Download Datasheet See All ANXA7 Products. Bring this labeled protein directly to your ...
more infohttps://www.creativebiomart.net/description_4523_17.htm

Propolis Reduces Phosphatidylcholine-Specific Phospholipase C Activity and Increases Annexin a7 Level in Oxidized-LDL...Propolis Reduces Phosphatidylcholine-Specific Phospholipase C Activity and Increases Annexin a7 Level in Oxidized-LDL...

H. Li, S. Huang, S. Wang et al., "Relationship between annexin A7 and integrin 4 in autophagy," International Journal of ... H. Li, S. Huang, S. Wang et al., "Targeting annexin A7 by a small molecule suppressed the activity of phosphatidylcholine- ... Propolis Reduces Phosphatidylcholine-Specific Phospholipase C Activity and Increases Annexin a7 Level in Oxidized-LDL- ...
more infohttps://www.hindawi.com/journals/ecam/2014/465383/ref/

Targeting annexin A7 by a small molecule suppressed the activity of phosphatidylcholine-specific phospholipase C in vascular...Targeting annexin A7 by a small molecule suppressed the activity of phosphatidylcholine-specific phospholipase C in vascular...

Targeting annexin A7 by a small molecule suppressed the activity of phosphatidylcholine-specific phospholipase C in vascular ... and further identified ABO as an inhibitor of annexin A7 (ANXA7) GTPase. Based on these findings, we hypothesize that ANXA7 is ...
more infohttps://www.sigmaaldrich.com/catalog/papers/24052074

Product Overview anti-Annexin VII AntibodiesProduct Overview anti-Annexin VII Antibodies

Selected quality suppliers for anti-Annexin VII antibodies. ... Order monoclonal and polyclonal Annexin VII antibodies for many ... Protein level used designations for anti-Annexin A7 (ANXA7) Antibodies annexin VII , annexin-7 , annexin A7 , synexin , annexin ... Mouse (Murine) Annexin A7 (ANXA7) interaction partners * Study demonstrates that annexin A7 protein has an important role in ... anti-Annexin A7 (ANXA7) Antibodies. Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding ...
more infohttps://www.antibodies-online.com/abstract/Annexin+A7+

Tissue expression of ANXA7 - Summary - The Human Protein AtlasTissue expression of ANXA7 - Summary - The Human Protein Atlas

Annexin A7. Protein classi. Protein class the gene product belongs to according to selected gene lists. List of protein classes ... Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains ... Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal ... Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, including ...
more infohttp://www.proteinatlas.org/ENSG00000138279-ANXA7/tissue

Gene InfoGene Info

annexin A7. Protein Similarities Based on Shared Motif Content. Find gene products sharing protein motifs with: NP_004025 NP_ ...
more infohttps://cgap.nci.nih.gov/Genes/GeneInfo?ORG=Hs&CID=631827

DNA polymerase delta p50 Antibody Pair (H00005425-AP21): Novus BiologicalsDNA polymerase delta p50 Antibody Pair (H00005425-AP21): Novus Biologicals

DNA polymerase delta p50 Antibody Pair. Matched antibody pairs validated for ELISA or IP-Western Blot. Backed by our 100% Guarantee.
more infohttps://www.novusbio.com/products/dna-polymerase-delta-p50-antibody-pair_h00005425-ap21

Anxa7 - PrimePCR Assay and Template | Life Science | Bio-RadAnxa7 - PrimePCR Assay and Template | Life Science | Bio-Rad

Annexin A7 Assay Type: SYBR® Green Assay Design: exonic Application: Gene Expression Unique Assay ID: qRnoCED0002713 ... Annexin A7 Assay Type: Probe Assay Design: exonic Application: Gene Expression Unique Assay ID: qRnoCEP0024544 FAM HEX TEX 615 ... Annexin A7 Assay Type: EvaGreen Application: Gene Expression Unique Assay ID: dRnoEG5125653 Info: EG; Same primer pair as used ... Annexin A7 Assay Type: Probe Application: Gene Expression Unique Assay ID: dRnoCPE5147284 Info: FAM; Same primer pair and probe ...
more infohttp://www.bio-rad.com/en-us/prime-pcr-assays/gene/anxa7-rat

AAGCCAT MIR135A MIR135BAAGCCAT MIR135A MIR135B

annexin A7. API5. 8539. API5. apoptosis inhibitor 5. ARHGAP6. 395. ARHGAP6. Rho GTPase activating protein 6. ...
more infohttp://software.broadinstitute.org/gsea/msigdb/cards/AAGCCAT_MIR135A_MIR135B.html

ANXA7 - PCR Primer Pair - SYBR | PrimePCR | Bio-RadANXA7 - PCR Primer Pair - SYBR | PrimePCR | Bio-Rad

annexin A7 Aliases:. ANX7, SNX, SYNEXIN RefSeq:. NC_000010.10 NT_030059.13 Ensembl:. ENSG00000138279 ... Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains ... Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique highly hydrophobic N-terminal ... Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties including ...
more infohttp://www.bio-rad.com/en-us/prime-pcr-assays/assay/qhsacid0006741-primepcr-sybr-green-assay-anxa7-human

ANXA7 purified MaxPab mouse polyclonal antibody (B01P) - (H00000310-B01P) - Products - AbnovaANXA7 purified MaxPab mouse polyclonal antibody (B01P) - (H00000310-B01P) - Products - Abnova

Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains ... Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal ... Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, including ... annexin A7. *Omim ID:. *186360. *Gene Ontology:. *Hyperlink. *Gene Summary:. * ...
more infohttp://www.abnova.com/products/products_detail.asp?catalog_id=H00000310-B01P

Suppression of inducible cyclooxygenase 2 gene transcription by aspirin and sodium salicylate | PNASSuppression of inducible cyclooxygenase 2 gene transcription by aspirin and sodium salicylate | PNAS

Influence of annexin A7 on insulin sensitivity of cellular glucose uptake. Dong Luo, Abul Fajol, Anja T. Umbach, Angelika A. ...
more infohttps://www.pnas.org/content/96/9/5292/tab-article-info

GO Gene ListGO Gene List

Annexin A7. NM_004034. NM_001156. Gene Info. ANXA7. Annexin A7. AI922845. Gene Info. ... Annexin A8-like 2. NM_001630. Gene Info. AP2B1. Adaptor-related protein complex 2, beta 1 subunit. NM_001030006. NM_001282. ... Annexin A2. NM_001136015. NM_001002857. NM_001002858. NM_004039. Gene Info. ...
more infohttps://cgap.nci.nih.gov/Genes/GoGeneQuery?PAGE=1&ORG=Hs&GOID=0065008

MORF PRKAG1MORF PRKAG1

annexin A7. AP3B1. 8546. AP3B1. adaptor-related protein complex 3, bet.... AP3S1. 1176. AP3S1. adaptor-related protein complex ...
more infohttp://software.broadinstitute.org/gsea/msigdb/cards/MORF_PRKAG1.html

KEGG SSDB Paralog Search Result: mmu:234135KEGG SSDB Paralog Search Result: mmu:234135

mmu:11750 annexin A7 K17095 463 108 ( -) 30 0.322 90 -, mmu:140493 potassium intermediate/small conductance cal K04944 732 108 ... mmu:11744 annexin A11 K17095 503 156 ( -) 41 0.315 111 -, mmu:54614 pre-mRNA processing factor 40B K12821 870 156 ( -) 41 0.315 ...
more infohttp://www.kegg.jp/ssdb-bin/ssdb_paralog?org_gene=mmu:234135

KEGG SSDB Best Search Result: tpe:Tpen 1227KEGG SSDB Best Search Result: tpe:Tpen 1227

amj:102572772 annexin A7 K17095 462 104 ( -) 30 0.323 62 -, 1 ani:AN6342.2 hypothetical protein 968 104 ( -) 30 0.333 78 -, 1 ... asn:102373690 annexin A7 K17095 462 104 ( -) 30 0.323 62 -, 1 bag:Bcoa_3136 thiamine-phosphate pyrophosphorylase K00788 214 104 ...
more infohttp://www.kegg.jp/ssdb-bin/ssdb_best?org_gene=tpe:Tpen_1227

Hereditary Spherocytosis disease: Malacards - Research Articles, Drugs, Genes, Clinical TrialsHereditary Spherocytosis disease: Malacards - Research Articles, Drugs, Genes, Clinical Trials

Annexin A7. Protein Coding. 11.27. DISEASES inferred 15 GeneCards inferred via :. Publications (show sections) ...
more infohttps://www.malacards.org/card/hereditary_spherocytosis

nature.com searchnature.com search

Ubiquitin-protein ligase E3C promotes glioma progression by mediating the ubiquitination and degrading of Annexin A7 *Si-Jian ... article Ubiquitin-protein ligase E3C promotes glioma progression by mediating the ubiquitination and degrading of Annexin A7 . ...
more infohttps://www.nature.com/search?author=%22Xiao%20ZHANG%22&error=cookies_not_supported&code=bb2cda83-3536-4b39-99db-621c07271e66

Inhibition of Cation Channels in Human Erythrocytes by Spermine, The Journal of Membrane Biology | 10.1007/s00232-010-9310-1 |...Inhibition of Cation Channels in Human Erythrocytes by Spermine, The Journal of Membrane Biology | 10.1007/s00232-010-9310-1 |...

Accelerated clearance of Plasmodium-infected erythrocytes in sickle cell trait and Annexin-A7 deficiency ... At 200 μM spermine blunted the increase of intracellular Ca2+, cell shrinkage and annexin V binding following 48 h exposure of ... At 200 μM spermine blunted the increase of intracellular Ca2+, cell shrinkage and annexin V binding following 48 h exposure of ... cell membrane scrambling from annexin V binding and cation channel activity with whole-cell patch-clamp in human erythrocytes. ...
more infohttps://www.deepdyve.com/lp/springer-journals/inhibition-of-cation-channels-in-human-erythrocytes-by-spermine-RtEKjleAw1
  • We recently found a small chemical molecule (6-amino-2, 3-dihydro-3-hydroxymethyl-1, 4-benzoxazine, ABO) that could inhibit oxidized low-density lipoprotein (oxLDL)-induced apoptosis and promote autophagy in VECs, and further identified ABO as an inhibitor of annexin A7 (ANXA7) GTPase. (sigmaaldrich.com)
  • Cytosolic Ca2+ was estimated from Fluo-3 fluorescence, cell volume from forward scatter, cell membrane scrambling from annexin V binding and cation channel activity with whole-cell patch-clamp in human erythrocytes. (deepdyve.com)
  • At 200 μM spermine blunted the increase of intracellular Ca2+, cell shrinkage and annexin V binding following 48 h exposure of cells at +37°C. In contrast, short exposure (10-30 min) of cells to 2 mM spermine was accompanied by increased cytosolic Ca2+ and annexin binding. (deepdyve.com)
  • Annexin A7 deficiency potentiates cardiac NFAT activity promoting hypertrophic signaling. (sigmaaldrich.com)
  • Targeting annexin A7 by a small molecule suppressed the activity of phosphatidylcholine-specific phospholipase C in vascular endothelial cells and inhibited atherosclerosis in apolipoprotein E -/- mice," Cell Death and Disease , vol. 4, Article ID e806, 2013. (hindawi.com)