A member of the annexin family that is a substrate for a tyrosine kinase, ONCOGENE PROTEIN PP60(V-SRC). Annexin A2 occurs as a 36-KDa monomer and in a 90-KDa complex containing two subunits of annexin A2 and two subunits of S100 FAMILY PROTEIN P11. The monomeric form of annexin A2 was formerly referred to as calpactin I heavy chain.
Protein of the annexin family exhibiting lipid interaction and steroid-inducibility.
A protein of the annexin family isolated from human PLACENTA and other tissues. It inhibits cytosolic PHOSPHOLIPASE A2, and displays anticoagulant activity.
Protein of the annexin family with a probable role in exocytotic and endocytotic membrane events.
Protein of the annexin family originally isolated from the electric organ of the electric ray Torpedo marmorata. It has been found in a wide range of mammalian tissue where it is localized to the apical membrane of polarized EPITHELIAL CELLS.
An annexin family member that plays a role in MEMBRANE FUSION and signaling via VOLTAGE-DEPENDENT CALCIUM CHANNELS.
A protein of the annexin family that catalyzes the conversion of 1-D-inositol 1,2-cyclic phosphate and water to 1-D-myo-inositol 1-phosphate.
Family of calcium- and phospholipid-binding proteins which are structurally related and exhibit immunological cross-reactivity. Each member contains four homologous 70-kDa repeats. The annexins are differentially distributed in vertebrate tissues (and lower eukaryotes) and appear to be involved in MEMBRANE FUSION and SIGNAL TRANSDUCTION.
A family of highly acidic calcium-binding proteins found in large concentration in the brain and believed to be glial in origin. They are also found in other organs in the body. They have in common the EF-hand motif (EF HAND MOTIFS) found on a number of calcium binding proteins. The name of this family derives from the property of being soluble in a 100% saturated ammonium sulfate solution.
Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a serine moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and serine and 2 moles of fatty acids.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Quaternary ammonium analog of ethidium; an intercalating dye with a specific affinity to certain forms of DNA and, used as diiodide, to separate them in density gradients; also forms fluorescent complexes with cholinesterase which it inhibits.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.
Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
A cell line derived from cultured tumor cells.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Extracellular vesicles generated by the shedding of CELL MEMBRANE blebs.
A family of G-protein-coupled receptors that was originally identified by its ability to bind N-formyl peptides such as N-FORMYLMETHIONINE LEUCYL-PHENYLALANINE. Since N-formyl peptides are found in MITOCHONDRIA and BACTERIA, this class of receptors is believed to play a role in mediating cellular responses to cellular damage and bacterial invasion. However, non-formylated peptide ligands have also been found for this receptor class.
Proteins prepared by recombinant DNA technology.
A short pro-domain caspase that plays an effector role in APOPTOSIS. It is activated by INITIATOR CASPASES such as CASPASE 9. Isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Organic compounds that contain technetium as an integral part of the molecule. These compounds are often used as radionuclide imaging agents.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Established cell cultures that have the potential to propagate indefinitely.
A family of intracellular CYSTEINE ENDOPEPTIDASES that play a role in regulating INFLAMMATION and APOPTOSIS. They specifically cleave peptides at a CYSTEINE amino acid that follows an ASPARTIC ACID residue. Caspases are activated by proteolytic cleavage of a precursor form to yield large and small subunits that form the enzyme. Since the cleavage site within precursors matches the specificity of caspases, sequential activation of precursors by activated caspases can occur.
Fluorescent probe capable of being conjugated to tissue and proteins. It is used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.
An in situ method for detecting areas of DNA which are nicked during APOPTOSIS. Terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 prime OH ends of either single- or double-stranded DNA. The terminal deoxynucleotidyl transferase nick end labeling, or TUNEL, assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA FRAGMENTATION.
A product of the lysis of plasminogen (profibrinolysin) by PLASMINOGEN activators. It is composed of two polypeptide chains, light (B) and heavy (A), with a molecular weight of 75,000. It is the major proteolytic enzyme involved in blood clot retraction or the lysis of fibrin and quickly inactivated by antiplasmins.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
A family of transcription factors characterized by the presence of highly conserved calcineurin- and DNA-binding domains. NFAT proteins are activated in the CYTOPLASM by the calcium-dependent phosphatase CALCINEURIN. They transduce calcium signals to the nucleus where they can interact with TRANSCRIPTION FACTOR AP-1 or NF-KAPPA B and initiate GENETIC TRANSCRIPTION of GENES involved in CELL DIFFERENTIATION and development. NFAT proteins stimulate T-CELL activation through the induction of IMMEDIATE-EARLY GENES such as INTERLEUKIN-2.
A family of hoofed MAMMALS consisting of HORSES, donkeys, and zebras. Members of this family are strict herbivores and can be classified as either browsers or grazers depending on how they feed.
A CALCIUM and CALMODULIN-dependent serine/threonine protein phosphatase that is composed of the calcineurin A catalytic subunit and the calcineurin B regulatory subunit. Calcineurin has been shown to dephosphorylate a number of phosphoproteins including HISTONES; MYOSIN LIGHT CHAIN; and the regulatory subunits of CAMP-DEPENDENT PROTEIN KINASES. It is involved in the regulation of signal transduction and is the target of an important class of immunophilin-immunosuppressive drug complexes.
A rare transmissible encephalopathy most prevalent between the ages of 50 and 70 years. Affected individuals may present with sleep disturbances, personality changes, ATAXIA; APHASIA, visual loss, weakness, muscle atrophy, MYOCLONUS, progressive dementia, and death within one year of disease onset. A familial form exhibiting autosomal dominant inheritance and a new variant CJD (potentially associated with ENCEPHALOPATHY, BOVINE SPONGIFORM) have been described. Pathological features include prominent cerebellar and cerebral cortical spongiform degeneration and the presence of PRIONS. (From N Engl J Med, 1998 Dec 31;339(27))
Lipids, predominantly phospholipids, cholesterol and small amounts of glycolipids found in membranes including cellular and intracellular membranes. These lipids may be arranged in bilayers in the membranes with integral proteins between the layers and peripheral proteins attached to the outside. Membrane lipids are required for active transport, several enzymatic activities and membrane formation.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
A 44-kDa highly glycosylated plasma protein that binds phospholipids including CARDIOLIPIN; APOLIPOPROTEIN E RECEPTOR; membrane phospholipids, and other anionic phospholipid-containing moieties. It plays a role in coagulation and apoptotic processes. Formerly known as apolipoprotein H, it is an autoantigen in patients with ANTIPHOSPHOLIPID ANTIBODIES.
Heat- and storage-stable plasma protein that is activated by tissue thromboplastin to form factor VIIa in the extrinsic pathway of blood coagulation. The activated form then catalyzes the activation of factor X to factor Xa.
Collections of illustrative plates, charts, etc., usually with explanatory captions.
The systematic study of the complete complement of proteins (PROTEOME) of organisms.
Irregular microscopic structures consisting of cords of endocrine cells that are scattered throughout the PANCREAS among the exocrine acini. Each islet is surrounded by connective tissue fibers and penetrated by a network of capillaries. There are four major cell types. The most abundant beta cells (50-80%) secrete INSULIN. Alpha cells (5-20%) secrete GLUCAGON. PP cells (10-35%) secrete PANCREATIC POLYPEPTIDE. Delta cells (~5%) secrete SOMATOSTATIN.
The first cervical vertebra.
A broad class of substances containing carbon and its derivatives. Many of these chemicals will frequently contain hydrogen with or without oxygen, nitrogen, sulfur, phosphorus, and other elements. They exist in either carbon chain or carbon ring form.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
Methods used for detecting the amplified DNA products from the polymerase chain reaction as they accumulate instead of at the end of the reaction.

HIV-1 Gag shares a signature motif with annexin (Anx7), which is required for virus replication. (1/67)

Genetic and biochemical analyses of the Gag protein of HIV-1 indicate a crucial role for this protein in several functions related to viral replication, including viral assembly. It has been suggested that Gag may fulfill some of the functions by recruiting host cellular protein(s). In our effort to identify structural and functional homologies between Gag and cellular cytoskeletal and secretory proteins involved in transport, we observed that HIV-1 Gag contains a unique PGQM motif in the capsid region. This motif was initially noted in the regulatory domain of synexin the membrane fusion protein of Xenopus laevis. To evaluate the functional significance of the highly conserved PGQM motif, we introduced alanine (A) in place of individual residues of the PGQM and deleted the motif altogether in a Gag expression plasmid and in an HIV-1 proviral DNA. The proviral DNA containing mutations in the PGQM motif showed altered expression, assembly, and release of viral particles in comparison to parental (NL4-3) DNA. When tested in multiple- and single-round replication assays, the mutant viruses exhibited distinct replication phenotypes; the viruses containing the A for the G and Q residues failed to replicate, whereas A in place of the P and M residues did not inhibit viral replication. Deletion of the tetrapeptide also resulted in the inhibition of replication. These results suggest that the PGQM motif may play an important role in the infection process of HIV-1 by facilitating protein-protein interactions between viral and/or viral and cellular proteins.  (+info)

Annexin VII and annexin XI are tyrosine phosphorylated in peroxovanadate-treated dogs and in platelet-derived growth factor-treated rat vascular smooth muscle cells. (2/67)

The intraperitoneal administration of peroxovanadate results in the rapid accumulation of many tyrosine-phosphorylated proteins in the liver and kidney of treated animals. The availability of large pools of tyrosine-phosphorylated proteins derived from normal tissues facilitates the purification and identification of previously unknown targets for cellular tyrosine kinases. Using this procedure, we have thus far identified four proteins in the liver and kidney of peroxovanadate-treated dogs. Two of these, annexin VII and annexin XI, were novel and had not been previously reported to be substrates of tyrosine kinases while the remaining two, ezrin and clathrin, have been reported to be tyrosine phosphorylated in some cell culture systems. In the present study, isolated proteins were identified both by sequence analysis and immunological methods. Annexin VII and annexin XI are present in cultured rat vascular smooth muscle cells and both were tyrosine phosphorylated in response to a physiological ligand, platelet-derived growth factor-BB (PDGF-BB). Furthermore, the extent of tyrosine phosphorylation in response to PDGF-BB was augmented by the co-addition of peroxovanadate to cell cultures. In vitro phosphorylation assays showed that PDGF receptor, calcium-dependent tyrosine kinase (CADTK/Pyk-2), Src kinase, and epidermal growth factor receptor all were able to phosphorylate purified annexin VII and XI on tyrosine residues. These findings confirm the usefulness of phosphatase inhibition by peroxovanadate as a tool for identifying previously unknown physiological targets for cellular protein tyrosine kinases.  (+info)

Defects in inositol 1,4,5-trisphosphate receptor expression, Ca(2+) signaling, and insulin secretion in the anx7(+/-) knockout mouse. (3/67)

The mammalian anx7 gene codes for a Ca(2+)-activated GTPase, which supports Ca(2+)/GTP-dependent secretion events and Ca(2+) channel activities in vitro and in vivo. To test whether anx7 might be involved in Ca(2+) signaling in secreting pancreatic beta cells, we knocked out the anx7 gene in the mouse and tested the insulin-secretory properties of the beta cells. The nullizygous anx7 (-/-) phenotype is lethal at embryonic day 10 because of cerebral hemorrhage. However, the heterozygous anx7 (+/-) mouse, although expressing only low levels of ANX7 protein, is viable and fertile. The anx7 (+/-) phenotype is associated with a substantial defect in insulin secretion, although the insulin content of the islets, is 8- to 10-fold higher in the mutants than in the normal littermate control. We infer from electrophysiological studies that both glucose-stimulated secretion and voltage-dependent Ca(2+) channel functions are normal. However, electrooptical recordings indicate that the (+/-) mutation has caused a change in the ability of inositol 1,4,5-trisphosphate (IP(3))-generating agonists to release intracellular calcium. The principle molecular consequence of lower anx7 expression is a profound reduction in IP(3) receptor expression and function in pancreatic islets. The profound increase in islets, beta cell number, and size may be a means of compensating for less efficient insulin secretion by individual defective pancreatic beta cells. This is a direct demonstration of a connection between glucose-activated insulin secretion and Ca(2+) signaling through IP(3)-sensitive Ca(2+) stores.  (+info)

Annexins VII and XI are present in a human macrophage-like cell line. Differential translocation on FcR-mediated phagocytosis. (4/67)

We have studied the divalent cation-dependent association of proteins to subcellular fractions of human macrophage-like cells before and after FcR-mediated phagocytosis. Among these proteins we have identified annexins VII and XI for the first time in these cells, along with annexins I, III, and VI. Although all of these annexins are present in the cytosolic fraction, the extent of their association to membrane and phagosome fractions from resting and stimulated cells is variable. Annexin VII translocates from cytosolic to membrane fractions after phagocytic stimulation, along with annexin I, III, and VI. Annexins VII and XI are found associated with purified phagosomes along with I, III, and VI, and this association is greater after a 24-h chase period. Our results show differences in the intracellular distribution of different annexins in macrophage-like cells on phagocytosis. Annexins VII, VI, III, and I respond to particle ingestion by translocating to phagosomes and other cell membrane structures, whereas annexin XI translocates predominantly to phagosomes, suggesting dissimilarities in their function.  (+info)

The sorcin-annexin VII calcium-dependent interaction requires the sorcin N-terminal domain. (5/67)

Surface plasmon resonance experiments show that at neutral pH the stability of the complex between sorcin and annexin VII (synexin) increases dramatically between 3 and 6 microM calcium; at the latter cation concentration the K(D) value is 0.63 microM. In turn, the lack of complex formation between the sorcin Ca(2+) binding domain (33-198) and synexin maps the annexin binding site to the N-terminal region of the sorcin polypeptide chain. Annexin VII likewise employs the N-terminal domain, more specifically the first 31 amino acids, to interact with sorcin [Brownawell, A.M. and Creutz, C.E. (1997) J. Biol. Chem. 272, 22182-22190]. The interaction may involve similar structural motifs in the two proteins, namely GGYY and GYGG in sorcin and GYPP in synexin.  (+info)

The annexinopathies: a new category of diseases. (6/67)

The annexins are a family of highly homologous phospholipid binding proteins, which share a four-domain structure, with one member of the family - annexin VI - having a duplication consisting of eight domains. Thus far, ten annexins have been described in mammals. Although the biological functions of the annexins have not been definitively established, two human diseases involving annexin abnormalities ('annexinopathies') have been identified as of the time of writing. Overexpression of annexin II occurs in the leukocytes of a subset of patients having a hemorrhagic form of acute promyelocytic leukemia. Underexpression of annexin V occurs on placental trophoblasts in the antiphospholipid syndrome and in preeclampsia. Also, an animal model has been described in which annexin VII is underexpressed and is associated with disease, but the relevance of this animal model to human disease is not yet understood. Future research is likely to elucidate additional 'annexinopathies'.  (+info)

Activation of annexin 7 by protein kinase C in vitro and in vivo. (7/67)

Annexin 7, a Ca(2+)/GTP-activated membrane fusion protein, is preferentially phosphorylated in intact chromaffin cells, and the levels of annexin 7 phosphorylation increase quantitatively in proportion to the extent of catecholamine secretion. Consistently, various protein kinase C inhibitors proportionately reduce both secretion and phosphorylation of annexin 7 in these cells. In vitro, annexin 7 is quantitatively phosphorylated by protein kinase C to a mole ratio of 2.0, and phosphorylation is extraordinarily sensitive to variables such as pH, calcium, phospholipid, phorbol ester, and annexin 7 concentration. Phosphorylation of annexin 7 by protein kinase C significantly potentiates the ability of the protein to fuse phospholipid vesicles and lowers the half-maximal concentration of calcium needed for this fusion process. Furthermore, other protein kinases, including cAMP-dependent protein kinase, cGMP-dependent protein kinase, and protein-tyrosine kinase pp60(c-)(src), also label annexin 7 with high efficiency but do not have this effect on membrane fusion. In the case of pp60(c-)(src), we note that this kinase, if anything, modestly suppresses the membrane fusion activity of annexin 7. These results thus lead us to hypothesize that annexin 7 may be a positive mediator for protein kinase C action in the exocytotic membrane fusion reaction in chromaffin cells.  (+info)

ANX7, a candidate tumor suppressor gene for prostate cancer. (8/67)

The ANX7 gene is located on human chromosome 10q21, a site long hypothesized to harbor a tumor suppressor gene(s) (TSG) associated with prostate and other cancers. To test whether ANX7 might be a candidate TSG, we examined the ANX7-dependent suppression of human tumor cell growth, stage-specific ANX7 expression in 301 prostate specimens on a prostate tissue microarray, and loss of heterozygosity (LOH) of microsatellite markers at or near the ANX7 locus. Here we report that human tumor cell proliferation and colony formation are markedly reduced when the wild-type ANX7 gene is transfected into two prostate tumor cell lines, LNCaP and DU145. Consistently, analysis of ANX7 protein expression in human prostate tumor microarrays reveals a significantly higher rate of loss of ANX7 expression in metastatic and local recurrences of hormone refractory prostate cancer as compared with primary tumors (P = 0.0001). Using four microsatellite markers at or near the ANX7 locus, and laser capture microdissected tumor cells, 35% of the 20 primary prostate tumors show LOH. The microsatellite marker closest to the ANX7 locus showed the highest rate of LOH, including one homozygous deletion. We conclude that the ANX7 gene exhibits many biological and genetic properties expected of a TSG and may play a role in prostate cancer progression.  (+info)

Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. The alternative splicing event is tissue specific and the mRNA containing the cassette exon is prevalent in brain, heart and skeletal muscle. The transcripts also differ in their 3-non coding regions by the use of two alternative poly(A) signals. Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, ...
Calcium flux is required for the mammalian sperm acrosome reaction, an exocytotic event triggered by egg binding, which results in a dramatic rise in sperm intracellular calcium. Calcium-dependent membrane fusion results in the release of enzymes that facilitate sperm penetration through the zona pellucida during fertilization. We have characterized inositol 1,4,5-trisphosphate (IP3)-gated calcium channels and upstream components of the phosphoinositide signaling system in mammalian sperm. Peptide antibodies colocalized G alpha q/11 and the beta 1 isoform of phospholipase C (PLC beta 1) to the anterior acrosomal region of mouse sperm. Western blotting using a polyclonal antibody directed against purified brain IP3 receptor (IP3R) identified a specific 260 kD band in 1% Triton X-100 extracts of rat, hamster, mouse and dog sperm. In each species, IP3R immunostaining localized to the acrosome cap. Scatchard analysis of [3H]IP3 binding to rat sperm sonicates revealed a curvilinear plot with high ...
Rabbit polyclonal Annexin VII antibody validated for WB, IHC and tested in Human. With 1 independent review. Immunogen corresponding to recombinant fragment
SCAN HISTORY OWL11_0 2 100 NSINGLE OWL17_1 2 110 NSINGLE OWL18_0 1 115 NSINGLE OWL19_1 1 125 NSINGLE OWL21_1 1 125 NSINGLE OWL26_0 1 125 NSINGLE SPTR37_9f 2 237 NSINGLE INITIAL MOTIF SETS ANNEXIN1 Length of motif = 23 Motif number = 1 Annexin motif I - 1 PCODE ST INT KTKGVDEVTIVNILTNRSNAQRQ LUHU36 46 46 KTKGVDEVTIINILTNRSNEQRQ ANX2_CHICK 46 46 TVKGVDEATIIDILTKRNNAQRQ ANX1_CAVCU 56 56 MVKGVDEATIIDILTKRNNAQRQ LUHU 55 55 KGIGTDEATIIDIVTHRSNAQRQ ANX6_MOUSE 376 376 KGMGTDEETILKILTSRNNAQRQ ANX5_CHICK 29 29 KGIGTNEQAIIDVLTKRSNTQRQ ANX8_HUMAN 35 35 KGFGTDEQEIIDVLVGRSNQQRQ DROANNX 29 29 RGIGTDEKMLISILTERSNAQRQ ANX3_HUMAN 32 32 KGLGTDEDAIINVLAYRSTAQRQ ANX4_BOVIN 27 27 KGFGTDEQAIVDVVANRSNDQRQ HUMSNEXIN 177 177 TAKGVDEATIIDIMTTRTNAQRP ANX1_COLLI 51 51 ANNEXIN2 Length of motif = 17 Motif number = 2 Annexin motif II - 1 PCODE ST INT LKSALSGHLETVILGLL LUHU36 86 17 LKSALSGHLEAVILGLL ANX2_CHICK 86 17 LKKALTGHLEEVVLALL ANX1_CAVCU 96 17 LKKALTGHLEEVVLALL LUHU 95 17 LKSEISGDLARLILGLM ANX6_MOUSE 416 17 ...
1. If E, F are fields and F is a subring of E, show each q in Aut(E/F) permutes the roots in E of each nonzero p(x) in F[x]. Hint if p(x)=a0+a1x+a2x^2+. . . +anx^n then p(x) has at most n roots in E. show that for z in E,.
Cancer Research UK is a registered charity in England and Wales (1089464), Scotland (SC041666) and the Isle of Man (1103). A company limited by guarantee. Registered company in England and Wales (4325234) and the Isle of Man (5713F). Registered address: Angel Building, 407 St John Street, London EC1V 4AD ...
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis. Alternatively spliced transcript variants have been described. [provided by RefSeq, Aug 2010 ...
Complete information for ANXA13 gene (Protein Coding), Annexin A13, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
The anorectic anx/anx mouse exhibits disturbed feeding behavior and aberrances, including neurodegeneration, in peptidergic neurons in the appetite regulating hypothalamic arcuate nucleus. Poor feeding in infants, as well as neurodegeneration, are common phenotypes in human disorders caused by dysfunction of the mitochondrial oxidative phosphorylation system (OXPHOS). We therefore hypothesized that the anorexia and degenerative phenotypes in the anx/anx mouse could be related to defects in the OXPHOS. In this study, we found reduced efficiency of hypothalamic OXPHOS complex I assembly and activity in the anx/anx mouse. We also recorded signs of increased oxidative stress in anx/anx hypothalamus, possibly as an effect of the decreased hypothalamic levels of fully assembled complex I, that were demonstrated by native Western blots. Furthermore, the Ndufaf1 gene, encoding a complex I assembly factor, was genetically mapped to the anx interval and found to be down-regulated in anx/anx mice. These ...
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This graph shows the total number of publications written about Annexin A3 by people in this website by year, and whether Annexin A3 was a major or minor topic of these publications ...
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Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 has 45 to 59% identity with other members of its family and shares a similar size and exon-intron organization. Isolated from human placenta, ANX4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells ...
Invitrogen™ eBioscience™ Annexin V, Recombinant Protein Unlabeled; 300μg Invitrogen™ eBioscience™ Annexin V, Recombinant Protein...
Dr. Seegers responded: Sympts off anx meds. MeghanS006~ the anti-anx meds. .are they SSRIs? If so ask if your |a href=/topics/depression track_data={
Annexin A2兔多克隆抗体(ab90643)可与人样本反应并经WB, IHC实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
ADVENTRX Pharmaceuticals, Inc. (NYSE Amex ANX) today announced that it has completed the previously announced sale of shares of convertible preferred stock purs
In our hands, the trypsin actually reduced the Annexin staining presumably by stripping off all the phosphotidyl-serine on the surface along with the surface proteins (adhesion molecules). The only time we have seen enhanced annexin is when the cells suffer enough trauma in harvesting that the membrane is not intact and the Annexin gets inside the cell and attaches to the ps on the inner membrane. Julie At 02:11 PM 8/18/98, you wrote: , ,I have a client who is staining with Annexin-FITC. They are looking at ,cell lines and are using trypsin to get the cells off the flasks. We are ,seeing what we think is an exagerated Annexin response. Does anyone out ,there have any feedback on how trypsin affects Annexin staining? Any ,experience and or information about this will be greatly appreciated. , Thanks in advance, , Joan Kalnitsky ,Joan Kalnitsky ,Flow Cytometry Laboratory ,VMRCVM ,(540) 231-4115 ,FAX 540-231-7367 , , It is better to serve than to receive , B. Borg , , , , , ...
|p|Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry
Annexin A11 Antibody 10479-2-AP has been identified with IF, IHC, IP, WB, ELISA. 10479-2-AP detected 56-60 kDa band in HeLa cells with 1:500-1:2000 dilution...
Bachem offers H-3196 Annexin A1 (1-25) (dephosphorylated) (human) for your research. Find all specific details here. Find product specific information including available pack sizes, CAS, detailed description and references here.
The anx/anx mouse displays poor appetite and lean appearance and is considered a good model for the study of anorexia nervosa. To identify new genes involved in feeding behavior and body weight regulation we performed an expression profiling in the hypothalamus of the anx/anx mice. Using commercial microarrays we detected 156 differentially expressed genes and validated 92 of those using TaqMan low-density arrays. The expression of a set of 87 candidate genes selected based on literature evidences was also quantified by TaqMan low-density arrays. Our results showed enrichment in deregulated genes involved in cell death, cell morphology and cancer as well as an alteration of several signaling circuits involved in energy balance including neuropeptide Y and melanocortin signaling. The expression profile along with the phenotype led us to conclude that anx/anx mice resemble the anorexia-cachexia syndrome typically observed in cancer, infection with human immunodeficiency virus or chronic diseases, ...
BioAssay record AID 502910 submitted by ChEMBL: Induction of annexin 2-mediated cytoskeleton aggregate accumulation in human HepG2 cells at 4 uM after 2 hrs by confocal laser scanning microscopy.
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Annexin A2 is a calcium regulated protein involved in membrane binding and actin polymerization. In this paper, Kevin Moreau and colleagues identified Annexin A2 as a…. ...
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Lung lamellar bodies, the storage organelles for lung surfactant phosphatidylcholine (PC), maintain an acidic pH that can be increased with weak bases. This study investigates the effect of a weak base, methylamine, on the pH in lamellar bodies and on the trafficking and packaging of newly synthesized PC in lamellar bodies. Methylamine increased the pH of isolated lung lamellar bodies and of lamellar bodies in intact cells. Metabolic labelling of isolated type II cells with [methyl-3H]choline showed that although methylamine (2.5-10 mM) did not alter the labelling of cellular or microsomal PC and disaturated PC, it decreased the labelling of the PC and disaturated PC in lamellar bodies. The packaging of PC in lamellar bodies (the specific activities ratio between the PC in lamellar bodies and the microsomal PC) also decreased in a time- and concentration-dependent manner. The cellular synthesis of PC or its packaging into lamellar bodies was unaltered by brefeldin A, suggesting that the Golgi ...
The Annexin family of calcium-binding proteins is composed of al least thirteen mammalian genes (Annexin A1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. The Annexin family
Invitrogen™ eBioscience™ Annexin V Apoptosis Detection Kit PerCP-eFluor™ 710 200 tests Invitrogen™ eBioscience™ Annexin V...
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Rabbit polyclonal Annexin VII antibody validated for WB, IHC and tested in Human. With 1 independent review. Immunogen ... Annexin A7 antibody. *Annexin VII antibody. *Annexin-7 antibody. *ANX7 antibody. *ANXA7 antibody ... All lanes : Anti-Annexin VII antibody (ab97323) at 1/2000 dilution. Lane 1 : 293T whole cell lysate. Lane 2 : A431 whole cell ... A pair of annexin repeats may form one binding site for calcium and phospholipid. ...
annexin A7. Protein family. Cancer-related genes, Mapped to neXtProt, Mapped to UniProt SWISS-PROT, Predicted intracellular ... Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains ... Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal ... Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, including ...
Record removed. This record was removed as a result of standard genome annotation processing. See the genome build documentation at http://www.ncbi.nlm.nih.gov/genome/guide/build.shtml for further information, or contact [email protected] ...
A pair of annexin repeats may form one binding site for calcium and phospholipid. UniProt ...
Annexin A7 (Anxa7) is a cytoskeletal protein interacting with Ca(2+) signaling which in turn is a crucial factor for cardiac ... Annexin A7 deficiency potentiates cardiac NFAT activity promoting hypertrophic signaling.. [Jakob Voelkl, Ioana Alesutan, ... These observations point to an important role of annexin A7 in the regulation of cardiac NFAT activity and hypertrophic ...
H. Li, S. Huang, S. Wang et al., "Relationship between annexin A7 and integrin 4 in autophagy," International Journal of ... H. Li, S. Huang, S. Wang et al., "Targeting annexin A7 by a small molecule suppressed the activity of phosphatidylcholine- ... Propolis Reduces Phosphatidylcholine-Specific Phospholipase C Activity and Increases Annexin a7 Level in Oxidized-LDL- ...
... ANXA7 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 150mM NaCl, 1mM DTT ... Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins. Annexin VII has molecular ... Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties including ...
Targeting annexin A7 by a small molecule suppressed the activity of phosphatidylcholine-specific phospholipase C in vascular ... and further identified ABO as an inhibitor of annexin A7 (ANXA7) GTPase. Based on these findings, we hypothesize that ANXA7 is ...
Recombinant Human annexin A7 produced inE.coliis a non-glycosylated, polypeptide chain containing amino acids (1-466) and ... ANXA7 annexin A7 [ Homo sapiens ]. Synonyms:. ANXA7; annexin A7; SNX; ANX7; SYNEXIN; Annexin A7; Annexin-7; Annexin VII; ... Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins. Annexin VII has molecular ... Recombinant Human Annexin A7, T7-tagged. Download Datasheet See All ANXA7 Products. Bring this labeled protein directly to your ...
A unique class of these proteins - annexins - can bind to certain membrane phospholipids in a Ca2+-dependent manner, providing ... By forming networks on the membrane surface, annexins can function as organizers of membrane domains and membrane-recruitment ... These and related properties enable annexins to participate in several otherwise unrelated events that range from membrane ... now known as annexin A7). It also reports a hallmark activity for this annexin - Ca2+-dependent vesicle aggregation. ...
Selected quality suppliers for anti-Annexin VII antibodies. ... Order monoclonal and polyclonal Annexin VII antibodies for many ... Protein level used designations for anti-Annexin A7 (ANXA7) Antibodies annexin VII , annexin-7 , annexin A7 , synexin , annexin ... Mouse (Murine) Annexin A7 (ANXA7) interaction partners * Study demonstrates that annexin A7 protein has an important role in ... anti-Annexin A7 (ANXA7) Antibodies. Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding ...
Annexin A7. ≥380,8. 3. 624.83. 2. 44. H2Db. HAIRNSFQYL. 170749. Mtmr4. Myotubularin related protein 4. ≥207,3. ...
... annexin A4 K17095 ANXA7_11; annexin A7/11 K17095 ANXA7_11; annexin A7/11 K01539 ATP1A; sodium/potassium-transporting ATPase ... 79124 Anxa4; annexin A4 155423 Anxa7; annexin A7 290527 Anxa11; annexin A11 24211 Atp1a1; sodium/potassium-transporting ATPase ...
... annexin A4 K17095 ANXA7_11; annexin A7/11 K17095 ANXA7_11; annexin A7/11 K01539 ATP1A; sodium/potassium-transporting ATPase ... 11746 Anxa4; annexin A4 11750 Anxa7; annexin A7 11744 Anxa11; annexin A11 11928 Atp1a1; ATPase, Na+/K+ transporting, alpha 1 ...
GeneID: 310 ANXA7 annexin A7.. *GeneID: 368 ABCC6 ATP binding cassette subfamily C member 6. ...
Influence of annexin A7 on insulin sensitivity of cellular glucose uptake. Dong Luo, Abul Fajol, Anja T. Umbach, Angelika A. ...
Annexin A7. Protein classi. Protein class the gene product belongs to according to selected gene lists. List of protein classes ... Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains ... Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal ... Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, including ...
annexin A7. Protein Similarities Based on Shared Motif Content. Find gene products sharing protein motifs with: NP_004025 NP_ ...
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Annexin A7 induction of neuronal apoptosis via effect on glutamate release in a rat model of subarachnoid hemorrhage ... Annexin A7 (ANXA7) has been shown to regulate glutamate release. However, the role of ANXA7 in early brain injury after SAH has ...
annexin A7. protein-coding. ARSD. arylsulfatase D. protein-coding. ACAT1. acetyl-CoA acetyltransferase 1. protein-coding. ...
Annexin A7 Assay Type: SYBR® Green Assay Design: exonic Application: Gene Expression Unique Assay ID: qRnoCED0002713 ... Annexin A7 Assay Type: Probe Assay Design: exonic Application: Gene Expression Unique Assay ID: qRnoCEP0024544 FAM HEX TEX 615 ... Annexin A7 Assay Type: EvaGreen Application: Gene Expression Unique Assay ID: dRnoEG5125653 Info: EG; Same primer pair as used ... Annexin A7 Assay Type: Probe Application: Gene Expression Unique Assay ID: dRnoCPE5147284 Info: FAM; Same primer pair and probe ...
Recombinant Protein and Annexin Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available. ... Annexin A7. Annexin A7 ELISA Kit. Annexin A7 Recombinant. Annexin A7 Antibody ... Also known as Annexin A7 (Annexin VII) (Annexin-7).. Calcium/phospholipid-binding protein which promotes membrane fusion and is ... Annexin A1 isoform p35. Annexin A1 isoform p35 ELISA Kit. Annexin A1 isoform p35 Recombinant. Annexin A1 isoform p35 Antibody ...
... annexin A4 K17095 ANXA7_11; annexin A7/11 K17095 ANXA7_11; annexin A7/11 K17099 ANXA13; annexin A13 K07828 NRAS; GTPase NRas ... 100339298 ANXA4; annexin A4 100009511 ANXA11; annexin A11 100338267 ANXA7; annexin A7 100008843 ANXA13; annexin A13 100344241 ...
annexin A7. API5. 8539. API5. apoptosis inhibitor 5. ARHGAP6. 395. ARHGAP6. Rho GTPase activating protein 6. ...
annexin A7 Aliases:. ANX7, SNX, SYNEXIN RefSeq:. NC_000010.10 NT_030059.13 Ensembl:. ENSG00000138279 ... Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains ... Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique highly hydrophobic N-terminal ... Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties including ...
Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains ... Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal ... Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, including ... annexin A7. *Omim ID:. *186360. *Gene Ontology:. *Hyperlink. *Gene Summary:. * ...
Ubiquitin-protein ligase E3C promotes glioma progression by mediating the ubiquitination and degrading of Annexin A7 *Si-Jian ... article Ubiquitin-protein ligase E3C promotes glioma progression by mediating the ubiquitination and degrading of Annexin A7 . ...
Therefore, the expression of all annexins was examined in LβT2 gonadotrope cells. ANXA4, A5, A6, A7 and A11 were detected in L ... Annexin A1 is a novel target gene of gonadotropin-releasing hormone in LβT2 gonadotrope cells ... We previously demonstrated that the expression of annexin A5 (ANXA5) is stimulated by GnRH in gonadotropes and has a ...
Annexin A5 Protein Recombinant is available 3 times from supplier abbex at Gentaur.com shop ... Annexin A7 Protein Recombinant Suppplier: abbex. Price: 295.25 USD Annexin A8 Protein Recombinant Suppplier: abbex. Price: ... Annexin A5 Protein Recombinant. Annexin A5 Protein Recombinant is available 3 times from Abbex labs abx260555 , Annexin A5 ... Annexin A1 Protein Recombinant Suppplier: abbex. Price: 414.29 USD Annexin A9 Protein Recombinant Suppplier: abbex. Price: ...
  • Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. (antibodypedia.com)
  • Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins. (neuromics.com)
  • Annexins are a multigene family of Ca 2+ -regulated proteins that are characterized by a unique Ca 2+ - and membrane-binding module - the annexin core domain. (nature.com)
  • A unique class of these proteins - annexins - can bind to certain membrane phospholipids in a Ca 2+ -dependent manner, providing a link between Ca 2+ signalling and membrane functions. (nature.com)
  • By forming networks on the membrane surface, annexins can function as organizers of membrane domains and membrane-recruitment platforms for proteins with which they interact. (nature.com)
  • Liemann, S. & Lewit-Bentley, A. Annexins: a novel family of calcium- and membrane-binding proteins in search of a function. (nature.com)
  • Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. (antibodies-online.com)
  • Additionally we are shipping Annexin VII Proteins (16) and Annexin VII Kits (13) and many more products for this protein. (antibodies-online.com)
  • Annexin A7 modulates BAG4 and BAG4-binding proteins in mitochondrial apoptosis. (semanticscholar.org)
  • Annexins (ANXs) are widely expressed and structurally related proteins which play multiple biological roles in animals, plants, and fungi. (frontiersin.org)
  • Although ANXs have been localized to the cytosol and the cell membrane and the molecular basis of the four annexin repeats is well established, the in vivo roles of these proteins are still far from clear, particularly with regard to the filamentous fungi. (frontiersin.org)
  • Annexin is a known calcium (Ca 2+ )-phospholipid binding protein, and the annexin family includes more than 160 unique proteins present in more than 65 different species, ranging from fungi and protists to plants and higher vertebrates ( Gerke and Moss, 2002 ). (frontiersin.org)
  • Annexins are traditionally thought of as calcium-dependent phospholipid-binding proteins, but recent work suggests a more complex set of functions. (beds.ac.uk)
  • More than a thousand proteins of the annexin superfamily have been identified in major eukaryotic phyla, but annexins are absent from yeasts and prokaryotes. (beds.ac.uk)
  • Animal and fungal annexins also have non-homologous amino-terminal domains of varying length and sequence, which are responsible for the distinct localizations and specialized functions of the proteins through post-translational modification and binding to other proteins. (beds.ac.uk)
  • Annexin A8-like 2 (ANXA8L2) belongs to the annexin family of calcium-dependent phospholipid binding proteins. (neuromics.com)
  • F-actin is accumulated promptly at the site of mem- (https://www.weblio.jp/content/%E4%B8%80% 2+ brane disruption and annexins, phospholipid-interacting proteins with Ca -binding activity, E8%88%AC%E8%B2%A1%E5%9B%A3%E6% are also recruited to the injured site and contribute to membrane repair [3]. (deepdyve.com)
  • We found higher concentration of proteins involved in immune response (interleukin-1 receptor antagonist and annexin A1), and of moonlighting proteins acting as plasminogen receptors (glyceraldehyde-3-phosphate dehydrogenase, α-enolase, and annexin A2) in patients affected by the idiopathic non-histaminergic or hereditary angioedema with unknown origin with respect to healthy controls. (bvsalud.org)
  • Annexin A7 (Anxa7) is a cytoskeletal protein interacting with Ca(2+) signaling which in turn is a crucial factor for cardiac remodeling following cardiac injury. (sigmaaldrich.com)
  • Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties including voltage-sensitive calcium channel activity, ion selectivity and membrane fusion. (neuromics.com)
  • It harbours binding sites for cytoplasmic protein ligands that can be targeted to membranes through the annexin-core-mediated phospholipid interaction. (nature.com)
  • Study demonstrates that annexin A7 protein has an important role in gastric cancer. (antibodies-online.com)
  • Also known as Annexin A1 (Annexin I) (Annexin-1) (Calpactin II) (Calpactin-2) (Chromobindin-9) (Lipocortin I) (Lipocortin-like 33 kDa protein) (Phospholipase A2 inhibitory protein) (p35). (mybiosource.com)
  • Description This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. (gentaur.com)
  • A gene on chromosome 9q21.13 that encodes annexin 1, a calcium/phospholipid-binding protein that promotes membrane fusion and is involved in exocytosis. (thefreedictionary.com)
  • Annexin is a naturally occurring human protein, produced by recombinant techniques, which is the key component of the Apomate(TM) kit. (thefreedictionary.com)
  • A protein of the annexin family that catalyzes the conversion of 1-D-inositol 1,2-cyclic phosphate and water to 1-D-myo-inositol 1-phosphate. (umassmed.edu)
  • Annexin A6 (AnxA6) is a Ca2+-dependent membrane-binding protein involved in vesicular traffic. (edu.pl)
  • Clemen CS, Herr C, Lie AA, Noegel AA, Schröder R (2001) Annexin VII: an astrogial protein exhibiting a Ca2+-dependent subcellular distribution. (edu.pl)
  • We recently found a small chemical molecule (6-amino-2, 3-dihydro-3-hydroxymethyl-1, 4-benzoxazine, ABO) that could inhibit oxidized low-density lipoprotein (oxLDL)-induced apoptosis and promote autophagy in VECs, and further identified ABO as an inhibitor of annexin A7 (ANXA7) GTPase. (sigmaaldrich.com)
  • Annexin A7 caused an anti-apoptosis phenotype in mouse hepatocarcinoma cells. (semanticscholar.org)
  • Down-regulated expression of Annexin A7 induces apoptosis in mouse hepatocarcinoma cell line by the intrinsic mitochondrial pathway. (ptgcn.com)
  • Interfering with intracellular annexin function, by overexpressing mutants or by using RNA-interference-mediated downregulation, has different effects depending on the annexin being targeted. (nature.com)
  • At 200 μM spermine blunted the increase of intracellular Ca2+, cell shrinkage and annexin V binding following 48 h exposure of cells at +37°C. In contrast, short exposure (10-30 min) of cells to 2 mM spermine was accompanied by increased cytosolic Ca2+ and annexin binding. (deepdyve.com)
  • Annexins interact with various cell-membrane components that are involved in the structural organization of the cell, intracellular signaling by enzyme modulation and ion fluxes, growth control, and they can act as atypical calcium channels. (beds.ac.uk)
  • A pair of annexin repeats may form one binding site for calcium and phospholipid. (rcsb.org)
  • Contains 4 annexin repeats. (abcam.com)
  • The unique annexin core domain is made up of four similar repeats approximately 70 amino acids long, each of which usually contains a characteristic 'type 2' motif for binding calcium ions. (beds.ac.uk)
  • Plant annexins (the D family) make up a monophyletic cluster whose members generally lack amino-terminal domains and functional calcium-binding sites in their second and third repeats [ 7 ] (see below). (beds.ac.uk)
  • Cytosolic Ca2+ was estimated from Fluo-3 fluorescence, cell volume from forward scatter, cell membrane scrambling from annexin V binding and cation channel activity with whole-cell patch-clamp in human erythrocytes. (deepdyve.com)
  • Babiychuk EB, Drager A (2000) Annexins in cell membrane dynamics. (edu.pl)
  • In this case, Bredel and his co-authors discovered that a specific spliced form of the membrane-binding tumor suppressor annexin A7, or ANXA7, leads to the decreased breakdown of a receptor, EGFR, that contributes to the growth of tumor cells. (uab.edu)
  • OBJECTIVES: To assess whether coselection of EGFR gains on 7p12 and monosomy 10 in glioblastomas promotes tumorigenic epidermal growth factor (EGF) signaling through loss of the annexin A7 (ANXA7) gene on 10q21.1-q21.2 and whether ANXA7 acts as a tumor suppressor gene by regulating EGFR in glioblastomas. (luriechildrens.org)
  • Ferrarese and colleagues hypothesized that alternative splicing of annexin A7 ( ANXA7 ), a membrane-bound tumor suppressor that undergoes tissue-specific exon skipping in the brain, contributes to glioblastoma progression. (aacrjournals.org)
  • Tc-99m-hynic annexin is an experimental radiopharmaceutical in phase II studies for imaging cell death in vivo to provide non-invasive molecular characterization of tumors and early evaluation of tumor response to anti-cancer treatment. (thefreedictionary.com)
  • These include ALIX ( Q8WUM4 ) ( Vito,1999 ), TSG101 ( Q99816 ) ( Okumura,2009 ), Annexin A7 ( P20073 ) and A11 ( P50995 ) ( Satoh,2002 ), PLSCR3 ( Q9NRY6 ) and Sec31A ( O94979 ) ( Shibata,2008 ). (eu.org)
  • it is likely that nuclear annexin A1 is involved in DNA replication, especially DNA damage induced gene mutation, since DNA damage induced mutagenesis plays an important role in tumorigenesis. (intechopen.com)
  • Hsp90-beta and annexin A1 were investigated as prognostic factors because of their apparent association with tumorigenesis. (biomedcentral.com)
  • Annexin 1 regulates phospholipase A2 activity and thus may have anti-inflammatory activity. (thefreedictionary.com)
  • Antigen standard for annexin A7 (ANXA7), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. (creativebiomart.net)
  • Each annexin contains a second, highly varible region - the N-terminal interaction domain. (nature.com)
  • Annexin family members are regulators of such varied processes such as ion flux, endocytosis and exocytosis, and cellular adhesion. (neuromics.com)
  • Creutz CE (1992) The annexins and exocytosis. (edu.pl)
  • Annexin VII has molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. (neuromics.com)
  • Recombinant Human annexin A7 produced in E. coli is a non-glycosylated, polypeptide chain containing amino acids (1-466) and having a total molecular mass of 53.6 kda. (creativebiomart.net)
  • Satoh, Nakano, Shibata, Maki: The penta-EF-hand domain of ALG-2 interacts with amino-terminal domains of both annexin VII and annexin XI in a Ca2+-dependent manner. (antibodies-online.com)
  • An annexin V kit and tryptase were purchased from Sigma Aldrich (Shanghai, China). (biomedcentral.com)
  • Annexin A7 deficiency potentiates cardiac NFAT activity promoting hypertrophic signaling. (sigmaaldrich.com)
  • These observations point to an important role of annexin A7 in the regulation of cardiac NFAT activity and hypertrophic response following cardiac stress conditions. (sigmaaldrich.com)
  • Recombinant human annexin is the core targeting component of Theseus Imaging Corporation's Apomate(TM) imaging agent now under development for the noninvasive assessment of early response to anti-cancer treatment and for evaluation of myocardial damage in acute myocardial infarction and cardiac transplant rejection. (thefreedictionary.com)
  • Donnelly SR, Moss SE (1998) Functional analysis of the human annexin I and VI gene promoters. (edu.pl)
  • Recombinant Human Annexin-VII at a concentration of 0.1mg containing 10 mM Tris, pH 8.0, 0.1% Triton X-100, 0.002% NaN3 and 10mM DTT. (creativebiomart.net)
  • Crystal structure of human annexin I at 2.5 Å resolution. (nature.com)
  • We have previously identified the upregulation of annexin A1 and Hsp90-beta in 16 human bronchial epithelial (HBE) cell lines compared with the NCI-A549 and NCI-H446 with two-dimensional liquid chromatography-tandem mass spectrometry (2D-LC-MS/MS). However, the origin and natural expression of the clinical specimens of lung cancer remain unknown. (biomedcentral.com)
  • Swairjo, M. A. & Seaton, B. A. Annexin structure and membrane interactions: a molecular perspective. (nature.com)
  • The annexin genes have duplicated extensively and independently in several eukaryotic lineages, as seen from their molecular phylogeny, their gene structures and their chromosomal positions. (beds.ac.uk)
  • Donnelly SR, Moss SE (1997) Annexins in the secretory pathway. (edu.pl)
  • High annexin A7 expression is associated with poor differentiation in gastric cancer patients, and it may be a predictor for lymphatic metastasis. (antibodies-online.com)
  • Function, expression and localization of annexin A7 in platelets and red blood cells: insights derived from an annexin A7 mutant mouse. (saladgaffe.ml)
  • The expression levels of Hsp90-beta and annexin A1 were examined using immunohistochemistry, in-situ hybridization, and Western blot. (biomedcentral.com)
  • Analysis of site-specific conservation in the core domain suggests a role for certain buried residues in the calcium-channel activity of vertebrate annexins and in the structural stability of their core domains. (beds.ac.uk)
  • Dysferlin interacts B3%95%E4%BA%BA%E8%97%A4%E4%BA% 2+ 95%E7%AF%80%E9%83%8E%E8%A8%98% with negatively charged phospholipids in a Ca -binding manner and its genetic defects result E5%BF%B5%E5%A4%A7%E9%98%AA%E5%9F in limb girdle muscular dystrophy type 2B [4]. (deepdyve.com)
  • Targeting annexin A7 by a small molecule suppressed the activity of phosphatidylcholine-specific phospholipase C in vascular endothelial cells and inhibited atherosclerosis in apolipoprotein E -/- mice," Cell Death and Disease , vol. 4, Article ID e806, 2013. (hindawi.com)
  • 2010). Therefore, nuclear annexin A1 is thought to play an important role in cell proliferation and/or cell transformation. (intechopen.com)