A member of the annexin family that is a substrate for a tyrosine kinase, ONCOGENE PROTEIN PP60(V-SRC). Annexin A2 occurs as a 36-KDa monomer and in a 90-KDa complex containing two subunits of annexin A2 and two subunits of S100 FAMILY PROTEIN P11. The monomeric form of annexin A2 was formerly referred to as calpactin I heavy chain.
Protein of the annexin family exhibiting lipid interaction and steroid-inducibility.
A protein of the annexin family isolated from human PLACENTA and other tissues. It inhibits cytosolic PHOSPHOLIPASE A2, and displays anticoagulant activity.
Protein of the annexin family with a probable role in exocytotic and endocytotic membrane events.
Protein of the annexin family originally isolated from the electric organ of the electric ray Torpedo marmorata. It has been found in a wide range of mammalian tissue where it is localized to the apical membrane of polarized EPITHELIAL CELLS.
An annexin family member that plays a role in MEMBRANE FUSION and signaling via VOLTAGE-DEPENDENT CALCIUM CHANNELS.
A protein of the annexin family that catalyzes the conversion of 1-D-inositol 1,2-cyclic phosphate and water to 1-D-myo-inositol 1-phosphate.
Family of calcium- and phospholipid-binding proteins which are structurally related and exhibit immunological cross-reactivity. Each member contains four homologous 70-kDa repeats. The annexins are differentially distributed in vertebrate tissues (and lower eukaryotes) and appear to be involved in MEMBRANE FUSION and SIGNAL TRANSDUCTION.
A family of highly acidic calcium-binding proteins found in large concentration in the brain and believed to be glial in origin. They are also found in other organs in the body. They have in common the EF-hand motif (EF HAND MOTIFS) found on a number of calcium binding proteins. The name of this family derives from the property of being soluble in a 100% saturated ammonium sulfate solution.
Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a serine moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and serine and 2 moles of fatty acids.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Quaternary ammonium analog of ethidium; an intercalating dye with a specific affinity to certain forms of DNA and, used as diiodide, to separate them in density gradients; also forms fluorescent complexes with cholinesterase which it inhibits.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.
Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
A cell line derived from cultured tumor cells.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Extracellular vesicles generated by the shedding of CELL MEMBRANE blebs.
A family of G-protein-coupled receptors that was originally identified by its ability to bind N-formyl peptides such as N-FORMYLMETHIONINE LEUCYL-PHENYLALANINE. Since N-formyl peptides are found in MITOCHONDRIA and BACTERIA, this class of receptors is believed to play a role in mediating cellular responses to cellular damage and bacterial invasion. However, non-formylated peptide ligands have also been found for this receptor class.
Proteins prepared by recombinant DNA technology.
A short pro-domain caspase that plays an effector role in APOPTOSIS. It is activated by INITIATOR CASPASES such as CASPASE 9. Isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Organic compounds that contain technetium as an integral part of the molecule. These compounds are often used as radionuclide imaging agents.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Established cell cultures that have the potential to propagate indefinitely.
A family of intracellular CYSTEINE ENDOPEPTIDASES that play a role in regulating INFLAMMATION and APOPTOSIS. They specifically cleave peptides at a CYSTEINE amino acid that follows an ASPARTIC ACID residue. Caspases are activated by proteolytic cleavage of a precursor form to yield large and small subunits that form the enzyme. Since the cleavage site within precursors matches the specificity of caspases, sequential activation of precursors by activated caspases can occur.
Fluorescent probe capable of being conjugated to tissue and proteins. It is used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.
An in situ method for detecting areas of DNA which are nicked during APOPTOSIS. Terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 prime OH ends of either single- or double-stranded DNA. The terminal deoxynucleotidyl transferase nick end labeling, or TUNEL, assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA FRAGMENTATION.
A product of the lysis of plasminogen (profibrinolysin) by PLASMINOGEN activators. It is composed of two polypeptide chains, light (B) and heavy (A), with a molecular weight of 75,000. It is the major proteolytic enzyme involved in blood clot retraction or the lysis of fibrin and quickly inactivated by antiplasmins.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Institutional committees established to protect the welfare of animals used in research and education. The 1971 NIH Guide for the Care and Use of Laboratory Animals introduced the policy that institutions using warm-blooded animals in projects supported by NIH grants either be accredited by a recognized professional laboratory animal accrediting body or establish its own committee to evaluate animal care; the Public Health Service adopted a policy in 1979 requiring such committees; and the 1985 amendments to the Animal Welfare Act mandate review and approval of federally funded research with animals by a formally designated Institutional Animal Care and Use Committee (IACUC).
The protection of animals in laboratories or other specific environments by promoting their health through better nutrition, housing, and care.
The use of animals as investigational subjects.
Animals or humans raised in the absence of a particular disease-causing virus or other microorganism. Less frequently plants are cultivated pathogen-free.
Physiological processes in biosynthesis (anabolism) and degradation (catabolism) of LIPIDS.
A generic term for fats and lipoids, the alcohol-ether-soluble constituents of protoplasm, which are insoluble in water. They comprise the fats, fatty oils, essential oils, waxes, phospholipids, glycolipids, sulfolipids, aminolipids, chromolipids (lipochromes), and fatty acids. (Grant & Hackh's Chemical Dictionary, 5th ed)
An unsaturated fatty acid that is the most widely distributed and abundant fatty acid in nature. It is used commercially in the preparation of oleates and lotions, and as a pharmaceutical solvent. (Stedman, 26th ed)
Cells in the body that store FATS, usually in the form of TRIGLYCERIDES. WHITE ADIPOCYTES are the predominant type and found mostly in the abdominal cavity and subcutaneous tissue. BROWN ADIPOCYTES are thermogenic cells that can be found in newborns of some species and hibernating mammals.
A CELL LINE derived from human T-CELL LEUKEMIA and used to determine the mechanism of differential susceptibility to anti-cancer drugs and radiation.
Lipids, predominantly phospholipids, cholesterol and small amounts of glycolipids found in membranes including cellular and intracellular membranes. These lipids may be arranged in bilayers in the membranes with integral proteins between the layers and peripheral proteins attached to the outside. Membrane lipids are required for active transport, several enzymatic activities and membrane formation.
Collections of illustrative plates, charts, etc., usually with explanatory captions.
The systematic study of the complete complement of proteins (PROTEOME) of organisms.
The first cervical vertebra.
Methods to identify and characterize cancer in the early stages of disease and predict tumor behavior.
New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.

Localization and quantitation of cardiac annexins II, V, and VI in hypertensive guinea pigs. (1/118)

Annexins are characterized by Ca2+-dependent binding to phospholipids. Annexin II mainly participates in cell-cell adhesion and signal transduction, whereas annexins V and VI also seem to regulate intracellular calcium cycling. Their abundance and localization were determined in left ventricle (LV) and right ventricle (RV) from hypertensive guinea pigs, during the transition from compensatory hypertrophy to heart failure. Immunoblot analysis of annexins II, V, and VI revealed an increased accumulation (2.6-, 1.45-, and 2.3-fold, respectively) in LV from hypertensive guinea pigs and no modification in RV. Immunofluorescent labeling of annexins II, V, and VI; of Na+-K+-ATPase; and of sarcomeric alpha-actinin showed that in control LV and RV, 1) annexin II is present in nonmuscle cells; 2) annexins V and VI are mainly observed in the sarcolemma and intercalated disks of myocytes; 3) annexins II, V, and VI strongly label endothelial cells and adventitia of coronary arteries; and 4) annexin VI is present in the media. At the onset of heart failure, the most striking changes are the increased protein accumulation in LV and the very strong labeling of annexins II, V, and VI in interstitial tissue, suggesting a role in fibrosis development and cardiac remodeling.  (+info)

A conserved nuclear element with a role in mammalian gene regulation. (2/118)

Mammalian genomes contain numerous fragments of DNA that are derived from inactivated transposable elements. The accumulation and persistence of these elements is generally attributed to transposase activity rather than through possession or acquisition of a function of value to the host genome. Here we describe such a repetitive element, named ALF (forannexin VILINE-2fragment), comprising 130 bp of DNA derived from a LINE-2 sequence, which functions as a potent T-cell-specific silencer. The expansion of the DNA database arising as a result of the human genome sequencing project enabled us to identify ALF in, or close to, several well characterized genes including those for annexin VI, interleukin-4 and protein kinase C-beta. A systematic analysis of the entire LINE-2 sequence revealed that ALF, and not other regions of the LINE-2 sequence, was especially highly represented in the human genome. Acquisition of a function by this repetitive element may explain its abundance. These data show that a conserved fragment of an interspersed nuclear element has the potential to modulate gene expression, a discovery that has broad implications for the way in which we view so-called 'junk' DNA and our understanding of eukaryotic gene regulation.  (+info)

Differential expression of Ca(2+)-binding proteins on follicular dendritic cells in non-neoplastic and neoplastic lymphoid follicles. (3/118)

We studied the Ca(2+)-capture ability of follicular dendritic cells (FDCs) in tonsillar secondary lymphoid follicles (LFs) and the expression of six Ca(2+)-binding proteins (CBPs), caldesmon, S-100 protein, calcineurin, calbindin-D, calmodulin, and annexin VI in LFs of various lymphoid tissues and caldesmon and S-100 protein in neoplastic follicles of follicular lymphomas. First, Ca(2+)-capture cytochemistry revealed extensive Ca(2+) capture in the nuclei and cytoplasm of FDCs, but little or none in follicular lymphocytes. All six CBPs were localized immunohistochemically in the LFs and were always present in the basal light zone. Immunoelectron microscopic staining of FDCs was classified into two patterns: caldesmon was distributed in the peripheral cytoplasm like a belt; S-100 protein, calcineurin, calbindin-D, and calmodulin were distributed diffusely in the cytosol. Annexin VI was, however, negative on FDCs. Immunocytochemistry also demonstrated CBP-positive FDCs within FDC-associated clusters isolated from germinal centers. In situ hybridization revealed diffuse calmodulin mRNA expression throughout the secondary LFs. These data indicate that the CBPs examined may regulate Ca(2+) in the different subcellular sites of FDCs, and the roles of CBPs may be heterogeneous. We also investigated the distribution of caldesmon and S-100 protein in follicular lymphomas on paraffin-embedded tissue sections. FDCs within grades I and II neoplastic follicles clearly expressed caldesmon, but not S-100 protein, except a part of grade II neoplastic follicles. FDCs within grade III follicles showed no caldesmon, but frequently expressed S-100 protein. These results demonstrate that the caldesmon and S-100 protein staining patterns of grade I follicular lymphomas are different from those of grade III follicular lymphomas and suggest that FDC networks in grade I neoplastic follicles may be similar to those in the light zone within non-neoplastic follicles, FDC networks in grade III neoplastic follicles may be similar to those in dark and basal light zones within non-neoplastic follicles, and grade II follicles may be intermediate between grade I and grade III follicles.  (+info)

Affinity labeling of annexin VI with a triazine dye, Cibacron blue 3GA. Probable interaction of the dye with C-terminal nucleotide-binding site within the annexin molecule. (4/118)

Annexin VI (AnxVI) from porcine liver, a member of the annexin family of Ca(2+)- and membrane-binding proteins, has been shown to bind ATP in vitro with a K(d) in the low micromolar concentration range. However, this protein does not contain within its primary structure any ATP-binding consensus motifs found in other nucleotide-binding proteins. In addition, binding of ATP to AnxVI resulted in modulation of AnxVI function, which was accompanied by changes in AnxVI affinity to Ca2+ in the presence of ATP. Using limited proteolytic digestion, purification of protein fragments by affinity chromatography on ATP-agarose, and direct sequencing, the ATP-binding site of AnxVI was located in a C-terminal half of the AnxVI molecule. To further study AnxVI-nucleotide interaction we have employed a functional nucleotide analog, Cibacron blue 3GA (CB3GA), a triazine dye which is commonly used to purify multiple ATP-binding proteins and has been described to modulate their activities. We have observed that AnxVI binds to CB3GA immobilized on agarose in a Ca(2+)-dependent manner. Binding is reversed by EGTA and by ATP and, to a lower extent, by other adenine nucleotides. CB3GA binds to AnxVI also in solution, evoking reversible aggregation of protein molecules, which resembles self-association of AnxVI molecules either in solution or on a membrane surface. Our observations support earlier findings that AnxVI is an ATP-binding protein.  (+info)

Immunological development and cardiovascular function are normal in annexin VI null mutant mice. (5/118)

Annexins are calcium-binding proteins of unknown function but which are implicated in important cellular processes, including anticoagulation, ion flux regulation, calcium homeostasis, and endocytosis. To gain insight into the function of annexin VI, we performed targeted disruption of its gene in mice. Matings between heterozygous mice produced offspring with a normal Mendelian pattern of inheritance, indicating that the loss of annexin VI did not interfere with viability in utero. Mice lacking annexin VI reached sexual maturity at the same age as their normal littermates, and both males and females were fertile. Because of interest in the role of annexin VI in cardiovascular function, we examined heart rate and blood pressure in knockout and wild-type mice and found these to be identical in the two groups. Similarly, the cardiovascular responses of both sets of mice to septic shock were indistinguishable. We also examined components of the immune system and found no differences in thymic, splenic, or bone marrow lymphocyte levels between knockout and wild-type mice. This is the first study of annexin knockout mice, and the lack of a clear phenotype has broad implications for current views of annexin function.  (+info)

Mapping the site of interaction between annexin VI and the p120GAP C2 domain. (6/118)

Annexin VI is a Ca(2+)-dependent membrane and phospholipid binding protein. It mediates a protein-protein interaction with the Ras p21 regulatory protein p120GAP. In this study we have mapped the binding site of GAP within the annexin VI protein. Using Far Western overlay binding assays and cell lysate competition studies we have mapped the site of interaction to the inter-lobe linker region; amino acids 325-363. Finally, using a GST fusion protein corresponding to this linker region we have demonstrated that cellular loading of the fusion protein into Rat-1 fibroblasts by electroporation blocks the interaction and co-immunoprecipitation of annexin VI and GAP.  (+info)

Annexin VI participates in the formation of a reversible, membrane-cytoskeleton complex in smooth muscle cells. (7/118)

The plasmalemma of smooth muscle cells is periodically banded. This arrangement ensures efficient transmission of contractile activity, via the firm, actin-anchoring regions, while the more elastic caveolae-containing "hinge" regions facilitate rapid cellular adaptation to changes in cell length. Since cellular mechanics are undoubtedly regulated by components of the membrane and cytoskeleton, we have investigated the potential role played by annexins (a family of phospholipid- and actin-binding, Ca(2+)-regulated proteins) in regulating sarcolemmal organization. Stimulation of smooth muscle cells elicited a relocation of annexin VI from the cytoplasm to the plasmalemma. In smooth, but not in striated muscle extracts, annexins II and VI coprecipitated with actomyosin and the caveolar fraction of the sarcolemma at elevated Ca(2+) concentrations. Recombination of actomyosin, annexins, and caveolar lipids in the presence of Ca(2+) led to formation of a structured precipitate. Participation of all 3 components was required, indicating that a Ca(2+)-dependent, cytoskeleton-membrane complex had been generated. This association, which occurred at physiological Ca(2+) concentrations, corroborates our biochemical fractionation and immunohistochemical findings and suggests that annexins play a role in regulating sarcolemmal organization during smooth muscle contraction.  (+info)

Annexin VI: an intracellular target for ATP. (8/118)

Annexin VI (AnxVI), an Ca2+- and phospholipid-binding protein, interacts in vitro with ATP in a calcium-dependent manner. Experimental evidence indicates that its nucleotide-binding domain which is localized in the C-terminal half of the protein differs structurally from ATP/GTP-binding motifs found in other nucleotide-binding proteins. The amino-acid residues of AnxVI directly involved in ATP binding have not been yet defined. Binding of ATP to AnxVI induces changes in the secondary and tertiary structures of protein, affecting the affinity of AnxVI for Ca2+ and, in consequence, influencing the Ca2+-dependent activities of AnxVI: binding to F-actin and to membranous phospholipids, and self-association of the annexin molecules. These observations suggest that ATP is a functional ligand for AnxVI in vivo, and ATP-sensitive AnxVI may play the role of a factor coupling vesicular transport and calcium homeostasis to cellular metabolism.  (+info)

Annexin A6 (AnxA6) is a Ca2+-dependent membrane-binding protein involved in vesicular traffic. The likely participation of AnxA6 in the response of lymphocytes to Ca2+ signals has not been investigated yet. The present study focuses on intracellular relocation of AnxA6 in human Jurkat T lymphoblasts upon stimulation followed by transient increase of intracellular [Ca2+] and exocytosis of interleukin-2 (IL-2). Stimulation of the cells under different experimental conditions (by lowering pH and/or by rising extracellular [Ca2+] in the presence of ionomycin) induced time-dependent transients of intracellular [Ca2+] and concomitant changes in AnxA6 intracellular localization and in IL-2 secretion, with only minor effects on cell viability and apoptosis. In resting conditions (in the presence of EGTA or with no ionophore) AnxA6 was localized uniformly in the cytosol, whereas it translocated to vesicular structures beneath the plasma membrane within 5 min following stimulation of Jurkat T cells and ...
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis. Alternatively spliced transcript variants have been described. [provided by RefSeq, Aug 2010 ...
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Background: Visual impairment (VI) may affect the lives of children, adolescents and adults although the effects of VI on the former two groups may be taken for granted as they account for less than half the population ...
Background Good clinical evidence has been reported for the effect of PC6 acupuncture in preventing or attenuating postoperative and pregnancy related nausea. Our primary aim was to examine whether PC6 acupuncture during a period of chemotherapy could complement pharmacological treatment of nausea in cancer patients in the palliative stage of their disease.. Method We conducted a prospective observational pilot study to measure changes in nausea, and also explored the relationship between nausea, pain and constipation. Twelve patients suffering from nausea and four nausea free patients participated in the study. The nausea free patients were included because they had been troubled by nausea in a previous course of chemotherapy, despite medication with antiemetic drugs, and were about to start a new course of treatment. The patients rated their intensity of nausea, pain and constipation on a numerical rating scale before each of 10 treatment sessions with PC6 acupuncture over the course of three ...
The role of the calcium- and phospholipid-binding protein annexin I (ANXA1) in cell cycle regulation has been investigated in estrogen receptor (ER)-positive MCF-7 and ER-negative MDA-MB-231 breast tumor cell lines. In MCF-7 cells, ANXA1-targeting small interfering RNA (siRNA) reduced ANXA1 mRNA and protein levels and attenuated cell proliferation induced by FCS, estradiol, or epidermal growth factor. Well-characterized agonists for the known ANXA1 receptor, FPR2, including the ANXA1 N-terminal proteolytic product ANXA1(2-26), lipoxin A(4) (LXA(4)), and the synthetic peptide, Trp-Lys-Tyr-Met-Val-D-Met (WKYMVm), stimulated proliferation of MCF-7 and MDA-MB-231 cells that was attenuated by incubation with FPR2 antagonists WRW(4) (1 μM) or Boc2 (100 nM) or by siRNA against FPR2. FCS-induced mitogenic responses were attenuated by each of the FPR antagonists and by siRNA against FPR2 and, to a lesser extent, FPR1. LXA(4) increased phosphorylation of Akt, p70(S6K) but not ERK1/2. Increases in cyclin ...
Mouse anti Human Annexin A3 antibody, clone 4F1 recognizes human annexin A3, also known as Annexin III, Inositol 1,2-cyclic phosphate 2-ph
TY - GEN. T1 - Neurotrophic roles of the beta-thymosins in the development and regeneration of the nervous system. AU - Sun, Woong. AU - Kim, Hyun. PY - 2007/9/1. Y1 - 2007/9/1. N2 - Beta-thymosins (Tβs) are polypeptides abundant in the cytosol, nucleus, and extracellular space of many cell types. In the nervous system, the expression of Tβs is regulated during the development of the central nervous system and following neuronal insults in cell-type and brain-region dependent manners, which may be related to the function of Tβs in the growth and regeneration of the nervous system. Supporting such a proposition, overexpression of Tβs in neurons has been shown to modify the axonal branches in vivo and neurite branches in vitro. These neurite-modifying functions have been suggested to be due to the activity of Tβs to bind actin. In addition, we recently observed that Tβs suppressed the apoptotic neuronal death in chick embryos, and these functions might be mediated by the extracellularly ...
ROG has upped the game audio ante with an advanced differential circuit design. It includes an Op-Amp that combines two opposite-phased signals while filtering out noise interference during transmission, providing clear audio stream output with double the intensity. Moreover, SupremeFX on Maximus VI GENE also implements multi-approach shielding, with ELNA audio capacitors helping offer 8-channel HD sound thats equal in power, clarity, and range to dedicated sound cards. This is the first-ever onboard sound capable of true audiophile-grade performance, set to revolutionize the way you hear games, movies, music, and even other people via chat. Discover a whole new dimension of superior audio and get ready to rule. Click the button to learn more about SupremeFX!. ...
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Our list of cell biology-related antibodies has been created for your convenience. Click on any target for more information and ordering instructions! GST IKK alpha P38 MAPK Caspase 8 mTOR/FRAP ERK1 TARGET AIF AKT1/3 ALDH1 Annexin VI ARC/Arg3.1 ATF4/CREB-2 ATP1b2/Na+K+ATPase Bax Bax(Ser184) Beclin 1 Beta Actin Beta cat
Heroes VI is a fast-paced epic story where Angels plot to end -- once and for all -- an unfinished war with their ancient rivals, the Faceless.
CHAPTER VI IMPACT OF PRIORITY SECTOR LENDING 6.1 PRINCIPAL FACTORS THAT HAVE DIRECT IMPACT ON PRIORITY SECTOR LENDING 6.2 ASSOCIATION BETWEEN THE PROFILE VARIABLES AND IMPACT OF PRIORITY SECTOR CREDIT
Mouse Monoclonal Anti-ANXA1 Antibody against Human annexin A1. Validated for Immunofluorescence, Immunohistochemistry and Western Blot
In our hands, the trypsin actually reduced the Annexin staining presumably by stripping off all the phosphotidyl-serine on the surface along with the surface proteins (adhesion molecules). The only time we have seen enhanced annexin is when the cells suffer enough trauma in harvesting that the membrane is not intact and the Annexin gets inside the cell and attaches to the ps on the inner membrane. Julie At 02:11 PM 8/18/98, you wrote: , ,I have a client who is staining with Annexin-FITC. They are looking at ,cell lines and are using trypsin to get the cells off the flasks. We are ,seeing what we think is an exagerated Annexin response. Does anyone out ,there have any feedback on how trypsin affects Annexin staining? Any ,experience and or information about this will be greatly appreciated. , Thanks in advance, , Joan Kalnitsky ,Joan Kalnitsky ,Flow Cytometry Laboratory ,VMRCVM ,(540) 231-4115 ,FAX 540-231-7367 , , It is better to serve than to receive , B. Borg , , , , , ...
TY - JOUR. T1 - Annexin A2. T2 - Its molecular regulation and cellular expression in cancer development. AU - Wang, Chi Yun. AU - Lin, Chiou Feng. PY - 2014. Y1 - 2014. N2 - Annexin A2 (ANXA2) orchestrates multiple biologic processes and clinical associations, especially in cancer progression. The structure of ANXA2 affects its cellular localization and function. However, posttranslational modification and protease-mediated N-terminal cleavage also play critical roles in regulating ANXA2. ANXA2 expression levels vary among different types of cancers. With some cancers, ANXA2 can be used for the detection and diagnosis of cancer and for monitoring cancer progression. ANXA2 is also required for drug-resistance. This review discusses the feasibility of ANXA2 which is active in cancer development and can be a therapeutic target in cancer management.. AB - Annexin A2 (ANXA2) orchestrates multiple biologic processes and clinical associations, especially in cancer progression. The structure of ANXA2 ...
1HVD: Structural and electrophysiological analysis of annexin V mutants. Mutagenesis of human annexin V, an in vitro voltage-gated calcium channel, provides information about the structural features of the ion pathway, the voltage sensor and the ion selectivity filter
This graph shows the total number of publications written about Annexin A3 by people in this website by year, and whether Annexin A3 was a major or minor topic of these publications ...
ATP- and membrane-binding protein that probably controls membrane reorganization/tubulation upon ATP hydrolysis. Plays a role in early endosomal transport.
ATP- and membrane-binding protein that probably controls membrane reorganization/tubulation upon ATP hydrolysis. Plays a role in early endosomal transport.
Annexin A11 Antibody 10479-2-AP has been identified with IF, IHC, IP, WB, ELISA. 10479-2-AP detected 56-60 kDa band in HeLa cells with 1:500-1:2000 dilution...
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Annexin A2兔多克隆抗体(ab90643)可与人样本反应并经WB, IHC实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
Janakkalan nuorisopalvelut toimivat lähettävänä, vastaanottavana ja koordinoivana tahona eurooppalaisessa vapaaehtoispalvelussa (EVS). EVS- vapaaehtoisia vastaanottavat monenlaiset työyhteisöt. Vapaaehtoistehtäviä on tarjolla esimerkiksi nuoriso-, vammais-, kulttuuri- ja ympäristötyön parissa. Vapaaehtoiset asuvat yleensä vapaaehtoistyöpaikkansa yhteydessä asuntolassa tai soluasunnossa tai paikallisessa isäntäperheessä. EVS-vapaaehtoispalvelun rahoituksesta vastaa Euroopan unioni. EVS -jaksoon sisältyy muun muassa matkat kohdemaahan, täysi ylläpito kohdemaassa, taskuraha, kattava vakuutus, tuki paikallisen kielen opiskeluun ja lähettävän ja vastaanottavan tahon tuki koko projektin ajaksi. Osallistujan maksettavaksi voi jäädä pieni osa matkakustannuksista.. The European Voluntary service is a project supported by the European Union, in order to create links between the European countries and to give the European youngsters possibilities to go abroad while being voluntary ...
Annexin A1 is an intracellular calcium/phospholipid-binding protein that is involved in membrane organization and the regulation of the immune system. It has been attributed an anti-inflammatory role at various control levels, and recently we could show that annexin A1 externalization during secondary necrosis provides an important fail-safe mechanism counteracting inflammatory responses when the timely clearance of apoptotic cells has failed. As such, annexin A1 promotes the engulfment of dying cells and dampens the postphagocytic production of proinflammatory cytokines. In our current follow-up study, we report that exposure of annexin A1 during secondary necrosis coincided with proteolytic processing within its unique N-terminal domain by ADAM10. Most importantly, we demonstrate that the released peptide and culture supernatants of secondary necrotic, annexin A1-externalizing cells induced chemoattraction of monocytes, which was clearly reduced in annexin A1- or ADAM10-knockdown cells. Thus, ...
Annexin A2 is a calcium-dependent, phospholipid-binding protein found on many cell types. It consists of a short hydrophobic tail (Ser2-Asn32), which dictates its function, and a core domain (Phe33-Asp339), which is involved in phospholipid binding. Annexin A2 has been implicated in a number of biochemical processes, including cell proliferation, foetal immune tolerance, ion-channel activation, cell-cell interactions and the bridging of membranes. Annexin A2 is reported to be a powerful activator of plasminogen and, therefore, is implicated in many normal and pathological processes such as haemostasis and metastasis. Myeloid cell lines are used, extensively, to study many aspects of cellular proliferation, differentiation and function. In the present study, we have used flow cytometry and real-time PCR to investigate the role of annexin A2 expression in the proliferation and differentiation of a number of myeloid cell lines. The results demonstrated that annexin A2 expression was affected when ...
Early in the apoptosis process, phosphatidylserine (PS) becomes exposed on the cell surface by flipping from the inner to outer leaflet of the cytoplasmic membrane. This event is thought to be important for macrophage recognition of cells undergoing apoptosis, thus allowing the cells to be rapidly phagocytosed. The binding of Annexin V to phosphatidylserine is calcium-dependent, reversible, but very tight, with a Kd of approximately 5 x 10-10 M. At low PS concentration, a binding ratio of 8 annexins to one PS has been reported. These features make Annexin V conjugates ideal for identifying membrane changes associated with apoptosis, by using either flow cytometric or in situ labeling methods. TACS® Annexin V kits are supplied with an Annexin V conjugate, an optimized binding buffer and propidium iodide. Cells are harvested and washed, then incubated with an Annexin V conjugate in binding buffer, for 15 minutes at room temperature prior to direct analysis. Alternatively, a secondary streptavidin ...
Invitrogen™ eBioscience™ Annexin V, Recombinant Protein Unlabeled; 300μg Invitrogen™ eBioscience™ Annexin V, Recombinant Protein...
The Annexin family of calcium-binding proteins is composed of al least thirteen mammalian genes (Annexin A1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. The Annexin family
|p|Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry
Buy ANXA2 recombinant protein, Annexin A2 Recombinant Protein-NP_001002857 (MBS203687) product datasheet at MyBioSource, Recombinant Proteins. Application: SDS-PAGE
Bachem offers H-3196 Annexin A1 (1-25) (dephosphorylated) (human) for your research. Find all specific details here. Find product specific information including available pack sizes, CAS, detailed description and references here.
Escherichia coli hõlmab väga suurt bakterite populatsiooni, millel on väga kõrge geneetiline ja fenotüübiline mitmekesisus. E. coli isolaatide ja temaga suguluses olevate bakterite genoomide sekveneerimine on andnud alust kaaluda taksonoomilist ümberpaigutamist, kuid seda pole tehtud tema meditsiinilise olulisuse tõttu.[10] Escherichia coli on geneetiliselt kõige kirjum bakteriliik, kuna ainult 20% genoomist on ühine kõikidele tüvedele.[11] Evolutsiooniliselt peaksid Shigella perekonna liikmed klassifitseeruma E. coli tüvedena, kuid nad on märgitud hoopiski parafüleetilise rühmana.[12] Kuid seevastu E. coli tüvi K-12, keda kasutatakse sageli rekombinantse DNA tegemiseks, peaks kuuluma hoopis teise perekonda, kuna on piisavalt erinev tüüptüvest. Tüvi on liigi alarühm, kes on oma omadustelt väga ainulaadne ja erineb seega oluliselt teistest tüvedest. Need erinevused on sageli tuvastatavad ainult molekulaarsel tasemel, kuid sellegipoolest võivad nad põhjustada muutusi ...
Richter-Helm BioLogics will help Athera Biotechnologies with development and manufacturing of its cardiovascular product. The recombinant protein Annexin A5 is intended for the treatment of patients with acute coronary syndrome who are at risk for myocardial infarction. ...
Synonyms: Uteroglobin, Clara cell 17 kDa protein, Clara cell phospholipid-binding protein, CCPBP, Clara cells 10 kDa secretory protein, CC10, PCB-binding protein, Secretoglobin family 1A member 1, Scgb1a1, Ugb, Utg, UG, CC16, CCSP, PCB-BP. ...
Complete information for ANXA1 gene (Protein Coding), Annexin A1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Complete information for ANXA1 gene (Protein Coding), Annexin A1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Annexin V Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidyl-serine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. The one-step staining procedure takes only 10 minutes. Detection can be analyzed by flow cytometry or by fluorescence microscopy. |p>|br />|br />‧ Detection method- Flow cytometry (Ex = 543 nm; Em = 570 nm) and fluorescence microscopy |br>|br />‧ Sample type- Living cells (suspension and adherant)|br>|br />‧ Species reactivity- Mammalian |br />|br />|br />|p>|b>Features and Benefits|/b>|br>|br />‧ Simple one step staining procedure in 10 minutes|br>|br />‧ Fast and convenient|br>|br />‧ Cy3 shows brighter red fluorescence|br />|br />|br />|br />|p>|b>Kit Contents:|/b>|br>|br />Annexin V-Cy3|br>|br />1X Binding Buffer, Annexin V
Invitrogen™ eBioscience™ Annexin V Apoptosis Detection Kit PerCP-eFluor™ 710 200 tests Invitrogen™ eBioscience™ Annexin V...
TransDetect® Annexin V-EGFP/PI Cell Apoptosis Detection Kit,Cell Detection,Cell Culture and Detection,Products,Beijing TransGen Biotech Co.Ltd,OverviewContents& storageCitations & referencesRelated ImagesDownloadOverviewDescriptionThe Annexin V
Annexin 1 is an anti-inflammatory protein that plays a key role in innate immunity by modulating the activation of several types of cells, including neutrophils. Here we have developed a cleavage assay using tagged annexin 1 and observed marked activity in the membrane fraction of activated neutrophils. A combination of inhibitors, transfected cells, and proteomic analyses allowed us to identify proteinase 3 as the main enzyme responsible for this cleavage in the N terminus region of the protein, at least in the context of neutrophil activation. Because annexin 1 is an important endogenous anti-inflammatory mediator, blocking its cleavage by proteinase 3 would augment its homeostatic pro-resolving actions and could represent an opportunity for innovative anti-inflammatory drug discovery.. ...
Adsorption of Chromium on dye contaminated soil has been studied using batch adsorption technique. This study is carried out to examine adsorption capacity of Chromium on..
Annexin V is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Annexin V is a monomer, binds ATRX and EIF5B, and interacts with hepatitis B virus (HBV ...
During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca2+-dependent affinity for PS and therefore is used as a probe for detecting apoptosis., Annexin V-FITC Apoptosis Detection Reagent, GTX14082, Applications: FACS; Flow cytometry/FACS; CrossReactivity:
SCAN HISTORY OWL11_0 2 100 NSINGLE OWL17_1 2 110 NSINGLE OWL18_0 1 115 NSINGLE OWL19_1 1 125 NSINGLE OWL21_1 1 125 NSINGLE OWL26_0 1 125 NSINGLE SPTR37_9f 2 237 NSINGLE INITIAL MOTIF SETS ANNEXIN1 Length of motif = 23 Motif number = 1 Annexin motif I - 1 PCODE ST INT KTKGVDEVTIVNILTNRSNAQRQ LUHU36 46 46 KTKGVDEVTIINILTNRSNEQRQ ANX2_CHICK 46 46 TVKGVDEATIIDILTKRNNAQRQ ANX1_CAVCU 56 56 MVKGVDEATIIDILTKRNNAQRQ LUHU 55 55 KGIGTDEATIIDIVTHRSNAQRQ ANX6_MOUSE 376 376 KGMGTDEETILKILTSRNNAQRQ ANX5_CHICK 29 29 KGIGTNEQAIIDVLTKRSNTQRQ ANX8_HUMAN 35 35 KGFGTDEQEIIDVLVGRSNQQRQ DROANNX 29 29 RGIGTDEKMLISILTERSNAQRQ ANX3_HUMAN 32 32 KGLGTDEDAIINVLAYRSTAQRQ ANX4_BOVIN 27 27 KGFGTDEQAIVDVVANRSNDQRQ HUMSNEXIN 177 177 TAKGVDEATIIDIMTTRTNAQRP ANX1_COLLI 51 51 ANNEXIN2 Length of motif = 17 Motif number = 2 Annexin motif II - 1 PCODE ST INT LKSALSGHLETVILGLL LUHU36 86 17 LKSALSGHLEAVILGLL ANX2_CHICK 86 17 LKKALTGHLEEVVLALL ANX1_CAVCU 96 17 LKKALTGHLEEVVLALL LUHU 95 17 LKSEISGDLARLILGLM ANX6_MOUSE 416 17 ...
Hochwertige Annexin V FITC Kits für Apoptose Detektion mit Flow Cytometry. ✅ Namhafte Top-Hersteller im Vergleich. ✅ Top-Support bei Fragen.
BioAssay record AID 502910 submitted by ChEMBL: Induction of annexin 2-mediated cytoskeleton aggregate accumulation in human HepG2 cells at 4 uM after 2 hrs by confocal laser scanning microscopy.
Spetsiifiliste söötmete alla liigitatakse näiteks sporulatsioonisööde ning indikaatorsöötmed. Sporulatsioonisööde kujutab nälja tingimusi rakkudele. Toitainete puuduses läbivad diploidsed pärmirakud meioosi ning sporuleeruvad (spoor moodustades neli askospooriga eoskoti (eoskott). Diploidsed pagaripärmi rakud sporuleeruvad, kui nad satuvad lämmastiku- ja fermenteeritava süsiniku nälga. Pagaripärmi sporulatsioonisööde sisaldab 10 korda vähem pärmiekstrakti ja 40 korda vähem glükoosi kui rikas sööde. Indikaatorsöötmed võimaldavad kindlaks teha, kas antud tüvi on võimeline kasvama söötmesse lisatud suhkrul. Maltoosi indikaatorsööde võimaldab vahet teha tüvedel, mis fermenteerivad või ei fermenteeri maltoosi. Selleks kasutatakse rikkas söötmes süsiniku allikana maltoosi ning lisatakse värvainet bromo-kresool-punakaslilla (bromocresol purple). Maltoosi fermenteerivad rakud muudavad söötme pH-d ning koloonia ümber tekib kollane rõngas. Sööde ise aga on ...
Phospholipid-binding protein EhC2A mediates calcium-dependent translocation of transcription factor URE3-BP to the plasma membrane of Entamoeba histolytica ...
ANXA4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 321 amino acids (1-321 a.a.) and having a molecular mass of 36 kDa.
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Rabbit polyclonal Annexin-2/ANXA2 (phospho S26) antibody. Validated in WB and tested in Human. Immunogen corresponding to synthetic peptide.
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Annexin A2 is a calcium regulated protein involved in membrane binding and actin polymerization. In this paper, Kevin Moreau and colleagues identified Annexin A2 as a…. ...
It has been reported that Annexin A2 (ANXA2) is up-regulated in hepatocellular carcinoma (HCC), but the roles of ANXA2 in the migration and invasion of HCC ...
"Enhanced Muscular Dystrophy from Loss of Dysferlin Is Accompanied by Impaired Annexin A6 Translocation after Sarcolemmal ... Lennon NJ, Kho A, Bacskai BJ, Perlmutter SL, Hyman BT, Brown RH (2003). "Dysferlin interacts with annexins A1 and A2 and ... including annexin and MG53. Exactly how dysferlin contributes to membrane resealing is not clear, but biochemical evidence ...
... annexin a4 MeSH D12.776.157.125.050.100 - annexin a5 MeSH D12.776.157.125.050.110 - annexin a6 MeSH D12.776.157.125.050.120 - ... annexin a1 MeSH D12.776.157.125.050.060 - annexin a2 MeSH D12.776.157.125.050.070 - Annexin A3 MeSH D12.776.157.125.050.080 - ... annexin a7 MeSH D12.776.157.125.412.249 - calmodulin MeSH D12.776.157.125.412.311 - calnexin MeSH D12.776.157.125.412.374 - ...
... is a protein that in humans is encoded by the ANXA6 gene. Annexin VI belongs to a family of calcium-dependent ... "Entrez Gene: ANXA6 annexin A6". "Dysmorphology data for Anxa6". Wellcome Trust Sanger Institute. "Haematology data for Anxa6". ... It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Exon 21 of annexin VI is ... The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of ...
S100 calcium-binding protein A6 (S100A6) is a protein that in humans is encoded by the S100A6 gene. The protein encoded by this ... Sudo T, Hidaka H (1999). "Characterization of the calcyclin (S100A6) binding site of annexin XI-A by site-directed mutagenesis ... "Entrez Gene: S100A6 S100 calcium binding protein A6". Deloulme JC, Assard N, Mbele GO, Mangin C, Kuwano R, Baudier J (November ...
S100A6‏ (S100 calcium binding protein A6) هوَ بروتين يُشَفر بواسطة جين S100A6 في الإنسان.[1] ... Sudo T، Hidaka H (1999). "Characterization of the calcyclin (S100A6) binding site of annexin XI-A by site-directed mutagenesis ... "Entrez Gene: S100A6 S100 calcium binding protein A6". مؤرشف من الأصل في 05 ديسمبر 2010. الوسيط ,مسار أرشيف=. تم تجاهله (مساعدة ... S100A6, 2A9, 5B10, CABP, CACY, PRA, S100 calcium binding protein A6, S10A6. ...
Annexin A6 is a protein that in humans is encoded by the ANXA6 gene. Annexin VI belongs to a family of calcium-dependent ... "Entrez Gene: ANXA6 annexin A6". "Dysmorphology data for Anxa6". Wellcome Trust Sanger Institute. "Haematology data for Anxa6". ... It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Exon 21 of annexin VI is ... The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of ...
Annexin A6 overexpression reduced membrane injury, consistent with enhanced repair. Treatment with recombinant annexin A6 ... and annexin A6 was previously identified as a genetic modifier of muscle injury and disease. Annexin A6 forms the repair cap ... These data identify annexins as mediators of membrane-associated Ca2+ release during membrane repair and annexin A6 as a ... To elucidate how annexins facilitate repair, we visualized annexin cap formation during injury. We found that annexin cap size ...
In contrast, annexin A6 contains 2 annexin cores and thus 8 annexin-repeat domains (22). Annexin A6s duplicated structure ... A6+A6) or wild-type plus mutant (A6+A6E233A) annexin combinations. Mutation of E233 in annexin A6 acted in a dominant-negative ... annexin A1 (NM_010730), annexin A2 (NM_007585), annexin A6 (NM_013472), and annexin A6-encoding sequencing from multiple ... and A6, but was most evident for annexin A2 and A6 (Figure 3A). Over the 240 seconds of imaging, overexpression of annexin A6 ...
Vega: OTTMUSG6325 (Anxa6, annexin A6)*CCDS: 24705, 24705.1, 48799*OMIM: ANNEXIN A6; ANXA6*Gene Ontology: Anxa6 *Mouse Phenome ... annexin A6. Synonyms: Annexin VI, Anx6, AnxVI, Cabm, Camb. Gene nomenclature, locus information, and GO, OMIM, and PMID ...
Recombinant human Annexin A6 fused to His-tag at N-terminus was expressed inE.coliand purified by using conventional ... ANXA6 annexin A6 [ Homo sapiens ]. Synonyms:. annexin A6; ANX6; CBP68; ANXA6; p68; p70; CPB-II; annexin-6; calelectrin; ... Recombinant Human Annexin A6, His-tagged. Download Datasheet See All ANXA6 Products. Bring this labeled protein directly to ... Recombinant human Annexin A6 fused to His-tag at N-terminus was expressed inE.coliand purified by using conventional ...
Recently, we showed that in EGFR overexpressing A431 cells, which lack endogenous Annexin A6 (AnxA6), ectopic expression of ... Recently, we showed that in EGFR overexpressing A431 cells, which lack endogenous Annexin A6 (AnxA6), ectopic expression of ... Annexin A6 inhibits Ras signaling in breast cancer cells.. Abstract. Overexpression of epidermal growth factor receptor (EGFR) ... Recently, we showed that in EGFR overexpressing A431 cells, which lack endogenous Annexin A6 (AnxA6), ectopic expression of ...
Annexin A6 (AnxA6) has been implicated in cell signalling by contributing to the organisation of the plasma membrane. Here we ... Annexin A6 (AnxA6) has been implicated in cell signalling by contributing to the organisation of the plasma membrane. Here we ... Annexin A6 regulates interleukin-2-mediated T-cell proliferation. Immunology and Cell Biology, 94 (6), 543-553. ...
Depletion of annexin A5, annexin A6 and collagen X causes no gross changes in matrix vesicle mediated mineralization. Pubmed ID ... Depletion of annexin A5, annexin A6 and collagen X causes no gross changes in matrix vesicle mediated mineralization. Journal ...
ANXA6, ANX6, CBP68, 67 kDa calelectrin, Annexin A6, Annexin VI p68, AnnexinA6, AnnexinVI, ANX 6, ANX A6, ANXA 6, Calcium ... Annexin A6, 1-673aa, Human. Background. Annexin A6 belongs to a family of calcium-dependent membrane and phospholipid binding ... Recombinant Annexin A6 protein was expressed in E.coli and purified by using conventional chromatography techniques.. ... Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane- ...
Earlier studies implicated annexin A6 (AnxA6) to inhibit secretion and participate in the organization of the extracellular ... Annexins are a family of proteins that bind to phospholipids in a calcium-dependent manner. ... Annexin A6 is a scaffold for PKCα to promote EGFR inactivation. *M Koese, C Rentero, +13 authors T Grewal ... Annexin A6 inhibits Ras signalling in breast cancer cells. *S Vilá de Muga, P Timpson, +11 authors T Grewal ...
Annexin A6 (AnxA6) has been implicated in the regulation of endo-/exocytic pathways, cholesterol transport, and the formation ... Annexin A6 (AnxA6) has been implicated in the regulation of endo-/exocytic pathways, cholesterol transport, and the formation ... Role of hepatic Annexin A6 in fatty acid-induced lipid droplet formation ... CHOLESTEROL TRANSPORT; PHOSPHOLIPASE A(2); PROTEINS; MICE; DEGRADATION; TRAFFICKING; AUTOPHAGY; GOLGI; LIVER; VI; Annexin A6; ...
A unique class of these proteins - annexins - can bind to certain membrane phospholipids in a Ca2+-dependent manner, providing ... By forming networks on the membrane surface, annexins can function as organizers of membrane domains and membrane-recruitment ... These and related properties enable annexins to participate in several otherwise unrelated events that range from membrane ... Annexin A6 improves anti‐migratory and anti‐invasive properties of tyrosine kinase inhibitors in EGFR overexpressing human ...
Depletion of annexin A5, annexin A6, and collagen X causes no gross changes in matrix vesicle-mediated mineralization, but lack ... Numerous biochemical studies have pointed to an essential role of annexin A5 (AnxA5), annexin A6 (AnxA6), and collagen X in ... Depletion of annexin A5, annexin A6, and collagen X causes no gross changes in matrix vesicle-mediated mineralization, but lack ... annexin A6, and collagen X is not essential for physiological calcification of growth plate cartilage. Therefore, annexins and ...
Annexin A6 (AnxA6) is a Ca2+-dependent membrane-binding protein involved in vesicular traffic. The likely participation of ... Calcium- and proton-dependent relocation of annexin A6 in Jurkat T cells stimulated for interleukin-2 secretion. ... Grewal T, Enrich C (2006) Molecular mechanisms involved in Ras inactivation: the annexin A6-p120GAP complex. BioEssays 28: 1211 ... GTP-induced membrane binding and ion channel activity of annexin VI: is annexin VI a GTP biosensor? Biophys J 82: 2737-2745. ...
The objective of this study is to determine the role of annexins and fetuin-A in MV formati … ... Annexin A2 / metabolism* * Annexin A6 / metabolism* * Bone Matrix / drug effects * Bone Matrix / enzymology ... Annexin-mediated matrix vesicle calcification in vascular smooth muscle cells J Bone Miner Res. 2008 Nov;23(11):1798-805. doi: ... Blockade of annexin calcium channel activity by K201 significantly decreased ALP activity and reduced the ability of the MVs to ...
301 ANXA1; annexin A1 302 ANXA2; annexin A2 308 ANXA5; annexin A5 309 ANXA6; annexin A6 375 ARF1; ADP ribosylation factor 1 ... annexin A1 K17092 ANXA2; annexin A2 K16646 ANXA5; annexin A5 K17094 ANXA6; annexin A6 K07937 ARF1_2; ADP-ribosylation factor 1/ ...
Annexin A6. 37. 1,269.01. 1.38±0.03. 3.17x10-6. 22. P08123. COL1A2. Collagen α-2(I) chain. 34. 1,279.13. 0.72±0.13. 1.06x10-5. ... Immunohistochemical localization of annexins I, II (p36 and p11 subunits), IV, and VI in the human hippocampus. Am J Pathol. ... These included annexin A2 (ANXA2), 60S ribosomal protein L18 (RPL18), tubulin β chain (TUBB) and collagen α-1 chain (COL1A1). ... Gerke V, Creutz CE and Moss SE: Annexins: Linking Ca2+ signalling to membrane dynamics. Nat Rev Mol Cell Biol. 6:449-461. 2005. ...
Annexin A6 (HGNC Symbol). Entrez gene summary. Annexin VI belongs to a family of calcium-dependent membrane and phospholipid ... Annexin A6. Protein classi. Protein class the gene product belongs to according to selected gene lists. List of protein classes ... It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been ... The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of ...
Rabbit Polyclonal Anti-PKM2 Antibody cited in 11 publications. Validated: WB, Simple Western, ICC/IF, IHC, IHC-P. Tested Reactivity: Human, Mouse, Rat, and more.
Mouse Monoclonal Anti-p70 S6 Kinase/S6K Antibody (1E12.). Validated: WB, ELISA, ICC/IF, IHC-P. Tested Reactivity: Human. 100% Guaranteed.
... annexin A6/, ,Graphics CenterX=392.6666666666667 CenterY=422.0 Width=60.0 Height=20.0 ZOrder=32768 FontSize=10 ...
Annexin A6. gi,576697441. 0,00147718. 0,00481598. 0,017. 3,3. Post-translational modification, protein turnover, and chaperones ...
2014). Annexin A6 modifies muscular dystrophy by mediating sarcolemmal repair. Proc. Natl. Acad. Sci. U.S.A. 111, 6004-6009. ... Annexin-6 has also recently been demonstrated to be a strong genetic modifier of muscle membrane repair in sarcoglyan knockout ...
Vitamin A (all-trans retinol) and all-trans retinoid acid (ATRA) interacted with human annexin A6 (AnxA6) as evidenced by AnxA6 ...
Swaggart, K. A., et al. Annexin A6 modifies muscular dystrophy by mediating sarcolemmal repair. Proc Natl Acad Sci USA. 111, ... Jævnt fordelte sarkomerer visualiseres i DIC imaging (venstre). En myofiber udtrykker annexin A6 tagget med GFP (midten panel ...
Annexin A6. Protein Coding. 5.6. DISEASES inferred 15 12. CTNNBL1 Catenin Beta Like 1. Protein Coding. 4.86. DISEASES inferred ... An important gene associated with Papillary Hidradenoma is ANXA11 (Annexin A11), and among its related pathways/superpathways ...
Annexin A6 (AnxA6) belongs to the conserved annexin family - a group of Ca2 +-dependent membrane binding proteins. AnxA6 is the ... Annexin A6 in the liver: From the endocytic compartment to cellular physiology by Carlos Enrich; Carles Rentero; Thomas Grewal ... Keywords: Annexin A6; Autophagosomes; Ca2 +-binding proteins; Hepatocytes; Intracellular trafficking; Liver regeneration; ... Annexin A2 is involved in Ca2 +-dependent plasma membrane repair in primary human endothelial cells by Sophia Nina Koerdt; ...
Recombinant Protein and Annexin Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available. ... Annexin A6. Annexin A6 ELISA Kit. Annexin A6 Recombinant. Annexin A6 Antibody ... Annexin A1 isoform p35. Annexin A1 isoform p35 ELISA Kit. Annexin A1 isoform p35 Recombinant. Annexin A1 isoform p35 Antibody ... Annexin A1 isoform p37. Annexin A1 isoform p37 ELISA Kit. Annexin A1 isoform p37 Recombinant. Annexin A1 isoform p37 Antibody ...
S4). Many of the LLO-A647-punctae co-localised with specific annexin A6-positive assemblies verifying annexin A6 can be used to ... During the annexin response, very few GRAF1 carriers were detected, but following loss of the annexin A6 signal, an extensive ... Pore formation was detected using annexin A6, which is one of the most Ca2+ sensitive annexins and has been shown to ... annexin A6 served as an internal control for toxin overload in cells. In cells unable to repair the LLO induced damage, annexin ...
Annexin A6 Controls Neuronal Membrane Dynamics Throughout Chick Cranial Sensory Gangliogenesis 7:55 pm - 8:00 pm. Discussion ...
  • Annexin A6 is a protein that in humans is encoded by the ANXA6 gene. (wikipedia.org)
  • Recombinant Annexin A6 protein was expressed in E.coli and purified by using conventional chromatography techniques. (arp1.com)
  • By binding to the extracellular matrix protein collagen X and matrix vesicles, annexins were proposed to anchor matrix vesicles in the extracellular space of hypertrophic chondrocytes to initiate the calcification of cartilage. (uea.ac.uk)
  • Annexin A6 (AnxA6) is a Ca2+-dependent membrane-binding protein involved in vesicular traffic. (edu.pl)
  • Clemen CS, Herr C, Lie AA, Noegel AA, Schröder R (2001) Annexin VII: an astrogial protein exhibiting a Ca2+-dependent subcellular distribution. (edu.pl)
  • It harbours binding sites for cytoplasmic protein ligands that can be targeted to membranes through the annexin-core-mediated phospholipid interaction. (nature.com)
  • Also known as Annexin A1 (Annexin I) (Annexin-1) (Calpactin II) (Calpactin-2) (Chromobindin-9) (Lipocortin I) (Lipocortin-like 33 kDa protein) (Phospholipase A2 inhibitory protein) (p35). (mybiosource.com)
  • Description This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. (gentaur.com)
  • of the Annexin protein superfamily play key roles in sensory nervous system development throughout metazoans. (tripdatabase.com)
  • Under chemotherapy, the exosomes contain the protein annexin-A6, which is not present in the exosomes released from untreated tumors. (epfl.ch)
  • Here, we describe a relationship between the small Ca 2+ -dependent membrane-binding protein Annexin B9 (AnxB9), apical β Heavy -spectrin (β H ) and the multivesicular body (MVB) in Drosophila . (biologists.org)
  • The annexins are a protein family characterized by Ca 2+ -dependent interactions with cellular membranes. (biochemj.org)
  • Evaluation of a 4-protein serum biomarker panel-biglycan, annexin-A6, myeloperoxidase, and protein S100-A9 (B-AMP)-for the detection of esophageal adenocarcinoma. (upmc.edu)
  • Quantitative proteomic analysis in rectus abdominus skeletal muscle tissue collected at delivery revealed reduced protein content of mitochondrial complex I (C-I) subunits (NDUFS3, NDUFV2) and altered content of proteins involved in calcium homeostasis/signaling (calcineurin A, α1-syntrophin, annexin A4) in OGDM (n = 6) vs. ONGT (n = 6). (plos.org)
  • These studies identified a variant in annexin a6 (Anxa6), a calcium binding protein, in causing the respiratory and cardiac muscle defects. (parentprojectmd.org)
  • This ability correlated with these types of MVs' carriage of: a) increased quantities of annexins (which might act as nucleation sites for calcification), b) increased quantities of bone-morphogenic protein, and c) larger Ca contents. (aging-us.com)
  • Annexin A2 (calpactin I, chromobindin 8, p36, Lipocortin II, PAP-IV, or Protein I) is a cytoskeletal calcium-dependent phospholipid binding protein, which has been shown to be a mediator of cortocosteroid activity, a substrate for serine/threonine kinases and growth regulated tyrosine kinases, and may play a role in secretion. (ecmbiosciences.com)
  • Clone (M330) was generated from a full length recombinant rat Annexin A6 protein. (ecmbiosciences.com)
  • This antibody detects a 67 kDa* protein corresponding to the molecular mass of Annexin A6 on SDS-PAGE immunoblots of human Jurkat cells. (ecmbiosciences.com)
  • Adiponectin reduces annexin A6 protein whereas mRNA levels are not affected. (bvsalud.org)
  • Adiponectin-mediated activation of peroxisome proliferator-activated receptor alpha (PPARalpha) and AMP-activated protein kinase (AMPK) does not account for reduced annexin A6 expression. (bvsalud.org)
  • Further, fatty acids and lipopolysaccharide that are elevated in obesity do not influence annexin A6 protein levels. (bvsalud.org)
  • S100calcium binding protein A6 (S100a6) belongsto the S100 family. (creativebiomart.net)
  • The binding of S100a6 to S100B is stabilized by S100B-bound calcium and zinc.It associates with both Annexin XI and CacyBP (Calcyclin-binding protein). (creativebiomart.net)
  • CAP-50 (annexin XI) is a member of annexin family proteins originally identified and characterized as a target protein for calcyclin (H. Tokumitsu et al. (elsevier.com)
  • Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. (wikipedia.org)
  • Annexins are a family of membrane-associated Ca2+-binding proteins implicated in repair, and annexin A6 was previously identified as a genetic modifier of muscle injury and disease. (jci.org)
  • Annexins are a family of proteins that bind to phospholipids in a calcium-dependent manner. (semanticscholar.org)
  • Annexins are a multigene family of Ca 2+ -regulated proteins that are characterized by a unique Ca 2+ - and membrane-binding module - the annexin core domain. (nature.com)
  • A unique class of these proteins - annexins - can bind to certain membrane phospholipids in a Ca 2+ -dependent manner, providing a link between Ca 2+ signalling and membrane functions. (nature.com)
  • By forming networks on the membrane surface, annexins can function as organizers of membrane domains and membrane-recruitment platforms for proteins with which they interact. (nature.com)
  • Liemann, S. & Lewit-Bentley, A. Annexins: a novel family of calcium- and membrane-binding proteins in search of a function. (nature.com)
  • Dysferlin is also markedly enriched at membrane lesions with several additional proteins thought to be involved in membrane resealing, including annexin and MG53. (wikipedia.org)
  • We have investigated in vivo the influence of the pH i on the membrane association of human annexins A1, A2, A4, A5 and A6 tagged with fluorescent proteins, and characterized this interaction for endogenous annexins present in smooth muscle and HEK (human embryonic kidney)-293 cells biochemically and by immunofluorescence microscopy. (biochemj.org)
  • We also identified annexins, annexin-binding proteins, Rab proteins, and other proteins involved in membrane organization, vesicular trafficking, and late endosomal function, as well as apolipoprotein E, which participates in cholesterol transport, immunoregulation, and modulation of cell growth and differentiation. (asm.org)
  • Staining was performed using human and mouse anti-MAGE-A antibodies that recognize multiple MAGE-A proteins, including mouse Mage-a1/Mage-a2/Mage-a3/Mage-a5/Mage-a6/Mage-a8. (sciencemag.org)
  • The Annexin family of calcium-binding proteins is composed of al least thirteen mammalian genes (Annexin A1-13). (ecmbiosciences.com)
  • Annexin A6 inhibits Ras signaling in breast cancer cells. (garvan.org.au)
  • The blot was probed with mouse monoclonal anti-Annexin A6 antibody at 1:250 (lane 1) or 1:1000 (lane 2). (ecmbiosciences.com)
  • The cells were fixed in paraformaldehyde and permeabilized using NP-40, then labeled with mouse monoclonal Annexin A2 (C-terminal region). (ecmbio.com)
  • Treatment with recombinant annexin A6 protected against acute muscle injury in vitro and in vivo. (jci.org)
  • Moreover, administration of recombinant annexin A6 in a model of muscular dystrophy reduced serum creatinine kinase, a biomarker of disease. (jci.org)
  • 측면 퓨전 모델 제안 막 파열 포함 하는 dysferlin의 측면 모집을 통해 급속 하 게 고치는 막 7 , 모델을 막는 단백질 단백질 집계를 통해 작은 구멍 고치는 제안 하는 동안 (주로 annexins) 8 . (jove.com)
  • 수리 패치 모델에서 셀으로 빠른 Ca 2 + 유입 트리거 복잡 한 또는 "패치", 융합 함께 세포내 vesicles의 형성 하는 여러 단백질 (dysferlin, annexins, mitsugumin-53, 및 EDH 단백질)의 채용 또는 막의 사이트에서 리소좀 손상 4 , , 9 10 , , 11 12 . (jove.com)
  • Earlier studies implicated annexin A6 (AnxA6) to inhibit secretion and participate in the organization of the extracellular matrix. (semanticscholar.org)
  • Among these, the expression levels of annexin A2 and tubulin β chain, were most strongly upregulated in the fibroblasts of patients with GBA‑PD and PARK2‑PD, respectively. (spandidos-publications.com)
  • Corroborating their laboratory data, the researchers found increased levels of annexin-A6 also in the exosomes of breast cancer patients undergoing neoadjuvant chemotherapy. (epfl.ch)
  • Monocytes express high levels of annexin A6 that could inhibit cholesterol efflux and it was investigated whether the atheroprotective effects of adiponectin are accompanied by changes in annexin A6 levels. (bvsalud.org)
  • Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. (wikipedia.org)
  • Researchers found that the Annexin A6 molecule contained in extracellular vesicles (EVs) is secreted by cancer-associated fibroblasts (CAFs) and taken up by gastric cancer cells, resulting in resistance to anticancer drug treatments. (valerys-worlds.com)
  • In conclusion, Zn 2+ inhibits the mineralizing competence of intravesicular Ca 2+ -P(i)-phospholipid complexes and function of annexin channels, thereby controlling Ca 2+ influx into MVs, the formation of the first crystal phase inside the vesicles and initiation of mineralization. (nyu.edu)
  • This core domain enables Ca 2+ -bound annexins to peripherally dock onto membranes that contain negatively charged phospholipids. (nature.com)
  • Figure 2: Annexin assemblies on membranes. (nature.com)
  • We also found that annexin overexpression promoted external blebs enriched in Ca2+ and correlated with a reduction of intracellular Ca2+ at the injury site. (jci.org)
  • Interfering with intracellular annexin function, by overexpressing mutants or by using RNA-interference-mediated downregulation, has different effects depending on the annexin being targeted. (nature.com)
  • Annexin A6 (AnxA6) controls cholesterol and membrane transport in endo- and exocytosis,and modulates triglyceride accumulation and storage. (ub.edu)
  • Annexin A6 modulates chick cranial neural crest cell emigration. (umd.edu)
  • Annexin A6 (AnxA6) has been implicated in cell signalling by contributing to the organisation of the plasma membrane. (edu.au)
  • Evidence for annexin A6-dependent plasma membrane remodelling of lipid domains. (semanticscholar.org)
  • We show that hypoxia promotes the interaction of the recombinant annexin A2-S100A10 (p11) and annexin A6 with the plasma membrane. (biochemj.org)
  • Our results show that annexin A6 and the heterotetramer A2-S100A10 (but not annexins A1, A4 and A5) interact independently of Ca 2+ with the plasma membrane at pH 6.2 and 6.6. (biochemj.org)
  • Annexin A6 reverses transformation of A431 cells after overexpression, and this effect may involve annexin A6 targeting of p120 RasGAP to the plasma membrane to inactivate Ras. (ecmbiosciences.com)
  • Annexin A6 controls neuronal membrane dynamics throughout chick cranial sensory gangliogenesis Cranial sensory ganglia are components of the peripheral nervous system that possess a significant somatosensory role and include neurons within the trigeminal and epibranchial nerve bundles. (tripdatabase.com)
  • Babiychuk EB, Drager A (2000) Annexins in cell membrane dynamics. (edu.pl)
  • Annexin A6 (AnxA6) has been implicated in the regulation of endo-/exocytic pathways, cholesterol transport, and the formation of multifactorial signaling complexes in many different cell types. (uni-regensburg.de)
  • An important gene associated with Papillary Hidradenoma is ANXA11 (Annexin A11), and among its related pathways/superpathways are Tyrosine Kinases / Adaptors and Signaling by ERBB4 . (malacards.org)
  • Recently, we showed that in EGFR overexpressing A431 cells, which lack endogenous Annexin A6 (AnxA6), ectopic expression of AnxA6 stimulates membrane recruitment of p120GAP to modulate Ras signaling. (garvan.org.au)
  • Annexin VI stimulates endocytosis and is involved in the trafficking of low density lipoprotein to the prelysosomal compartment. (semanticscholar.org)
  • Extracellular annexin functions that have been substantiated by mouse knockout models are anti-inflammatory and fibrinolytic activities. (nature.com)
  • Figure 5: Annexins have specialized extracellular roles. (nature.com)
  • Numerous biochemical studies have pointed to an essential role of annexin A5 (AnxA5), annexin A6 (AnxA6), and collagen X in matrix vesicle-mediated biomineralization during endochondral ossification and in osteoarthritis. (uea.ac.uk)
  • The antibody has been shown to label Annexin A2 in immunocytochemical analysis of PC12 cells and immunohistochemical analysis of mouse diaphragm. (ecmbio.com)
  • Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis. (wikipedia.org)
  • Creutz CE (1992) The annexins and exocytosis. (edu.pl)
  • The Annexin family has been implicated as regulators of such diverse processes as ion-flux, endocytosis and exocytosis, and cellular adhesion. (ecmbiosciences.com)
  • Bandorowicz J, Pikula S, Sobota A (1992) Annexins IV (p32) and VI (p68) interact with erythrocyte membrane in a calcium-dependent manner. (edu.pl)
  • Hence, collagen X is needed for hematopoiesis during endochondral ossification and for the immune response, but the interaction of annexin A5, annexin A6, and collagen X is not essential for physiological calcification of growth plate cartilage. (uea.ac.uk)
  • Each annexin contains a second, highly varible region - the N-terminal interaction domain. (nature.com)
  • In conclusion, our results suggest that mineralization in VSMCs requires both active MVs and an interaction of the MVs with type I collagen, and both steps require annexin activity. (nih.gov)
  • The dimerization of annexin A2 within the annexin A2-S100A10 complex is essential for the pH-dependent membrane interaction at this pH range. (biochemj.org)
  • Annexin A6 overexpression reduced membrane injury, consistent with enhanced repair. (jci.org)
  • Figure 3: The regulation of membrane-actin interactions by annexin A2. (nature.com)
  • Annexin A6 regulates interleukin-2-mediated T-cell proliferation" by Rhea Cornely, Abigail H. Pollock et al. (edu.au)
  • Annexin II regulates multivesicular endosome biogenesis in the degradation pathway of animal cells. (semanticscholar.org)
  • Importantly, the researchers found that neutralizing annexin-A6 or blocking monocytes during chemotherapy prevents the experimental mammary tumors from metastasizing to the lung. (epfl.ch)
  • Annexin A6 in monocytes from overweight probands or type 2 diabetic patients is significantly elevated compared to monocytes of normal-weight controls. (bvsalud.org)
  • Therefore, the current study demonstrates that adiponectin reduces annexin A6 in monocytes and thereby may enhance cholesterol efflux. (bvsalud.org)
  • In agreement with these in vitro finding an increase of monocytic annexin A6 in type 2 diabetes monocytes was observed. (bvsalud.org)
  • Therefore, annexins and collagen X may rather fulfill functions in growth plate cartilage not directly linked to the mineralization process. (uea.ac.uk)
  • Blockade of annexin calcium channel activity by K201 significantly decreased ALP activity and reduced the ability of the MVs to subsequently calcify on collagen, whether the K201 was added during or after MV formation. (nih.gov)
  • Furthermore, cellular MVs had significantly increased ability to calcify on collagen compared with secreted MVs, likely because of their increased ALP activity and annexin II content but low fetuin-A content. (nih.gov)
  • Monocytic annexin A6 positively correlates with body mass index and negatively with systemic adiponectin of the blood donors. (bvsalud.org)
  • Further experimental results have suggested that Annexin A6 contributes to anticancer drug resistance by stabilizing the cell adhesion molecule β1 integrin on the gastric cancer cell membrane and activating FAK-YAP signaling after being taken up by gastric cancer cells. (valerys-worlds.com)
  • These and related properties enable annexins to participate in several otherwise unrelated events that range from membrane dynamics to cell differentiation and migration. (nature.com)
  • Banerjee P and Bandyopadhyay A . Cytosolic dynamics of annexin A6 triggers feedback regulation of hypertrophy via atrial natriuretic peptide in cardiomyocytes. (iicb.res.in)
  • The objective of this study is to determine the role of annexins and fetuin-A in MV formation and activity during calcification in bovine vascular smooth muscle cells (BVSMCs). (nih.gov)
  • Apoptosis was measured by annexin V and propidium iodide dye-based flowcytometry assay, mitochondrial membrane potential assay, and nuclear DNA fragmentation assay. (bvsalud.org)
  • It seems that loading of annexin-A6 into exosomes is significantly enhanced in response to chemotherapy," explains Ioanna Keklikoglou, first author of the study. (epfl.ch)
  • Upon reaching the lung, the exosomes release their content, including annexin-A6. (epfl.ch)
  • Further, we found that exosome-fibronectin binding is heparan sulfate-dependent, consistent with our observation that trabecular meshwork exosomes are enriched in the heparin/heparan sulfate binding annexins A2 and A6. (physiciansweekly.com)
  • In this way, dexamethasone-treated explants released exosomes with a significant reduction in annexin A2 and A6 per exosome. (physiciansweekly.com)
  • We found that annexin cap size positively correlated with increasing Ca2+ concentrations. (jci.org)
  • Gauthier-Kemper A, Suárez Alonso M, Sündermann F, Niewidok B, Fernandez MP, Bakota L, Heinisch JJ, Brandt R . Annexins A2 and A6 interact with the extreme N terminus of tau and thereby contribute to tau's axonal localization . (alzforum.org)
  • It is highly similar to human annexins I and II sequences, each of which contain four such repeats. (wikipedia.org)
  • Recombinant human Annexin A6 fused to His-tag at N-terminus was expressed in E.coli and purified by using conventional chromatography techniques.MW = 78.0 kDa (693aa). (creativebiomart.net)
  • Donnelly SR, Moss SE (1998) Functional analysis of the human annexin I and VI gene promoters. (edu.pl)
  • Crystal structure of human annexin I at 2.5 Å resolution. (nature.com)
  • Annexin A6 and Late Endosomal Cholesterol Modulate Integrin Recycling and Cell Migration. (semanticscholar.org)
  • Chander A, Sen N, Naidu DG, Spitzer AR (2003) Calcium ionophore and phorbol ester increase membrane binding of annexin A7 in alveolar type II cells. (edu.pl)
  • Some annexins can also occur extracellularly and can have functions outside cells. (nature.com)
  • Through gene expression analysis in gastric cancer cells, researchers discovered that CAF-EV derived Annexin A6 played an important role in gastric cancer cell resistance to anticancer drugs. (valerys-worlds.com)
  • The researchers also found that Annexin A6 is rarely expressed in gastric cancer cells themselves and is usually present only in CAFs. (valerys-worlds.com)
  • This shows that Annexin A6 is involved in the acquisition of anticancer drug resistance by being taken up by gastric cancer cells via EVs. (valerys-worlds.com)
  • Here we showed that CAF-derived Annexin A6, which surrounds gastric cancer cells, causes resistance to anticancer drug treatments," said research team member, Associate Professor Takatsugu Ishimoto. (valerys-worlds.com)
  • In the Thiel myeloma cell line, TPD52 bound to annexin VI in a Ca 2+ -dependent manner, suggesting that these molecules may act in concert to regulate secretory processes in plasma cells, similarly to what was observed in pancreatic acinar cells. (elsevier.com)
  • Immunocytochemical labeling of Annexin A2 in NGF-differentiated rat PC12 cells. (ecmbio.com)