Lipid Metabolism: Physiological processes in biosynthesis (anabolism) and degradation (catabolism) of LIPIDS.Lipids: A generic term for fats and lipoids, the alcohol-ether-soluble constituents of protoplasm, which are insoluble in water. They comprise the fats, fatty oils, essential oils, waxes, phospholipids, glycolipids, sulfolipids, aminolipids, chromolipids (lipochromes), and fatty acids. (Grant & Hackh's Chemical Dictionary, 5th ed)Annexin A1: Protein of the annexin family exhibiting lipid interaction and steroid-inducibility.Annexin A2: A member of the annexin family that is a substrate for a tyrosine kinase, ONCOGENE PROTEIN PP60(V-SRC). Annexin A2 occurs as a 36-KDa monomer and in a 90-KDa complex containing two subunits of annexin A2 and two subunits of S100 FAMILY PROTEIN P11. The monomeric form of annexin A2 was formerly referred to as calpactin I heavy chain.TriglyceridesOleic Acid: An unsaturated fatty acid that is the most widely distributed and abundant fatty acid in nature. It is used commercially in the preparation of oleates and lotions, and as a pharmaceutical solvent. (Stedman, 26th ed)Adipocytes: Cells in the body that store FATS, usually in the form of TRIGLYCERIDES. WHITE ADIPOCYTES are the predominant type and found mostly in the abdominal cavity and subcutaneous tissue. BROWN ADIPOCYTES are thermogenic cells that can be found in newborns of some species and hibernating mammals.Fatty Acids: Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)Hepatocytes: The main structural component of the LIVER. They are specialized EPITHELIAL CELLS that are organized into interconnected plates called lobules.Annexin A6: Protein of the annexin family with a probable role in exocytotic and endocytotic membrane events.Jurkat Cells: A CELL LINE derived from human T-CELL LEUKEMIA and used to determine the mechanism of differential susceptibility to anti-cancer drugs and radiation.Annexin A4: Protein of the annexin family originally isolated from the electric organ of the electric ray Torpedo marmorata. It has been found in a wide range of mammalian tissue where it is localized to the apical membrane of polarized EPITHELIAL CELLS.Annexin A5: A protein of the annexin family isolated from human PLACENTA and other tissues. It inhibits cytosolic PHOSPHOLIPASE A2, and displays anticoagulant activity.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.T-Lymphocytes: Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.Calcium: A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.Annexins: Family of calcium- and phospholipid-binding proteins which are structurally related and exhibit immunological cross-reactivity. Each member contains four homologous 70-kDa repeats. The annexins are differentially distributed in vertebrate tissues (and lower eukaryotes) and appear to be involved in MEMBRANE FUSION and SIGNAL TRANSDUCTION.Atlases as Topic: Collections of illustrative plates, charts, etc., usually with explanatory captions.Proteomics: The systematic study of the complete complement of proteins (PROTEOME) of organisms.Cervical Atlas: The first cervical vertebra.Early Detection of Cancer: Methods to identify and characterize cancer in the early stages of disease and predict tumor behavior.Neoplasms: New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.Breast Neoplasms: Tumors or cancer of the human BREAST.Prostatic Neoplasms: Tumors or cancer of the PROSTATE.Gene Expression Regulation, Neoplastic: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Antibody Specificity: The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Antibodies, Viral: Immunoglobulins produced in response to VIRAL ANTIGENS.Rabbits: The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.Antibodies, Bacterial: Immunoglobulins produced in a response to BACTERIAL ANTIGENS.Antibody Formation: The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.Antibodies, Neutralizing: Antibodies that reduce or abolish some biological activity of a soluble antigen or infectious agent, usually a virus.Fluorescent Antibody Technique: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.Antibody Affinity: A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes.Phosphatidylinositol 3-Kinases: Phosphotransferases that catalyzes the conversion of 1-phosphatidylinositol to 1-phosphatidylinositol 3-phosphate. Many members of this enzyme class are involved in RECEPTOR MEDIATED SIGNAL TRANSDUCTION and regulation of vesicular transport with the cell. Phosphatidylinositol 3-Kinases have been classified both according to their substrate specificity and their mode of action within the cell.MAP Kinase Signaling System: An intracellular signaling system involving the MAP kinase cascades (three-membered protein kinase cascades). Various upstream activators, which act in response to extracellular stimuli, trigger the cascades by activating the first member of a cascade, MAP KINASE KINASE KINASES; (MAPKKKs). Activated MAPKKKs phosphorylate MITOGEN-ACTIVATED PROTEIN KINASE KINASES which in turn phosphorylate the MITOGEN-ACTIVATED PROTEIN KINASES; (MAPKs). The MAPKs then act on various downstream targets to affect gene expression. In mammals, there are several distinct MAP kinase pathways including the ERK (extracellular signal-regulated kinase) pathway, the SAPK/JNK (stress-activated protein kinase/c-jun kinase) pathway, and the p38 kinase pathway. There is some sharing of components among the pathways depending on which stimulus originates activation of the cascade.Protein Kinases: A family of enzymes that catalyze the conversion of ATP and a protein to ADP and a phosphoprotein.Protein-Serine-Threonine Kinases: A group of enzymes that catalyzes the phosphorylation of serine or threonine residues in proteins, with ATP or other nucleotides as phosphate donors.Craniofacial Abnormalities: Congenital structural deformities, malformations, or other abnormalities of the cranium and facial bones.Facial Bones: The facial skeleton, consisting of bones situated between the cranial base and the mandibular region. While some consider the facial bones to comprise the hyoid (HYOID BONE), palatine (HARD PALATE), and zygomatic (ZYGOMA) bones, MANDIBLE, and MAXILLA, others include also the lacrimal and nasal bones, inferior nasal concha, and vomer but exclude the hyoid bone. (Jablonski, Dictionary of Dentistry, 1992, p113)Skull: The SKELETON of the HEAD including the FACIAL BONES and the bones enclosing the BRAIN.Morphogenesis: The development of anatomical structures to create the form of a single- or multi-cell organism. Morphogenesis provides form changes of a part, parts, or the whole organism.Regeneration: The physiological renewal, repair, or replacement of tissue.Jaw: Bony structure of the mouth that holds the teeth. It consists of the MANDIBLE and the MAXILLA.Branchial Region: A region, of SOMITE development period, that contains a number of paired arches, each with a mesodermal core lined by ectoderm and endoderm on the two sides. In lower aquatic vertebrates, branchial arches develop into GILLS. In higher vertebrates, the arches forms outpouchings and develop into structures of the head and neck. Separating the arches are the branchial clefts or grooves.Face: The anterior portion of the head that includes the skin, muscles, and structures of the forehead, eyes, nose, mouth, cheeks, and jaw.Neural Crest: The two longitudinal ridges along the PRIMITIVE STREAK appearing near the end of GASTRULATION during development of nervous system (NEURULATION). The ridges are formed by folding of NEURAL PLATE. Between the ridges is a neural groove which deepens as the fold become elevated. When the folds meet at midline, the groove becomes a closed tube, the NEURAL TUBE.Guided Tissue Regeneration: Procedures for enhancing and directing tissue repair and renewal processes, such as BONE REGENERATION; NERVE REGENERATION; etc. They involve surgically implanting growth conducive tracks or conduits (TISSUE SCAFFOLDING) at the damaged site to stimulate and control the location of cell repopulation. The tracks or conduits are made from synthetic and/or natural materials and may include support cells and induction factors for CELL GROWTH PROCESSES; or CELL MIGRATION.

Localization and quantitation of cardiac annexins II, V, and VI in hypertensive guinea pigs. (1/118)

Annexins are characterized by Ca2+-dependent binding to phospholipids. Annexin II mainly participates in cell-cell adhesion and signal transduction, whereas annexins V and VI also seem to regulate intracellular calcium cycling. Their abundance and localization were determined in left ventricle (LV) and right ventricle (RV) from hypertensive guinea pigs, during the transition from compensatory hypertrophy to heart failure. Immunoblot analysis of annexins II, V, and VI revealed an increased accumulation (2.6-, 1.45-, and 2.3-fold, respectively) in LV from hypertensive guinea pigs and no modification in RV. Immunofluorescent labeling of annexins II, V, and VI; of Na+-K+-ATPase; and of sarcomeric alpha-actinin showed that in control LV and RV, 1) annexin II is present in nonmuscle cells; 2) annexins V and VI are mainly observed in the sarcolemma and intercalated disks of myocytes; 3) annexins II, V, and VI strongly label endothelial cells and adventitia of coronary arteries; and 4) annexin VI is present in the media. At the onset of heart failure, the most striking changes are the increased protein accumulation in LV and the very strong labeling of annexins II, V, and VI in interstitial tissue, suggesting a role in fibrosis development and cardiac remodeling.  (+info)

A conserved nuclear element with a role in mammalian gene regulation. (2/118)

Mammalian genomes contain numerous fragments of DNA that are derived from inactivated transposable elements. The accumulation and persistence of these elements is generally attributed to transposase activity rather than through possession or acquisition of a function of value to the host genome. Here we describe such a repetitive element, named ALF (forannexin VILINE-2fragment), comprising 130 bp of DNA derived from a LINE-2 sequence, which functions as a potent T-cell-specific silencer. The expansion of the DNA database arising as a result of the human genome sequencing project enabled us to identify ALF in, or close to, several well characterized genes including those for annexin VI, interleukin-4 and protein kinase C-beta. A systematic analysis of the entire LINE-2 sequence revealed that ALF, and not other regions of the LINE-2 sequence, was especially highly represented in the human genome. Acquisition of a function by this repetitive element may explain its abundance. These data show that a conserved fragment of an interspersed nuclear element has the potential to modulate gene expression, a discovery that has broad implications for the way in which we view so-called 'junk' DNA and our understanding of eukaryotic gene regulation.  (+info)

Differential expression of Ca(2+)-binding proteins on follicular dendritic cells in non-neoplastic and neoplastic lymphoid follicles. (3/118)

We studied the Ca(2+)-capture ability of follicular dendritic cells (FDCs) in tonsillar secondary lymphoid follicles (LFs) and the expression of six Ca(2+)-binding proteins (CBPs), caldesmon, S-100 protein, calcineurin, calbindin-D, calmodulin, and annexin VI in LFs of various lymphoid tissues and caldesmon and S-100 protein in neoplastic follicles of follicular lymphomas. First, Ca(2+)-capture cytochemistry revealed extensive Ca(2+) capture in the nuclei and cytoplasm of FDCs, but little or none in follicular lymphocytes. All six CBPs were localized immunohistochemically in the LFs and were always present in the basal light zone. Immunoelectron microscopic staining of FDCs was classified into two patterns: caldesmon was distributed in the peripheral cytoplasm like a belt; S-100 protein, calcineurin, calbindin-D, and calmodulin were distributed diffusely in the cytosol. Annexin VI was, however, negative on FDCs. Immunocytochemistry also demonstrated CBP-positive FDCs within FDC-associated clusters isolated from germinal centers. In situ hybridization revealed diffuse calmodulin mRNA expression throughout the secondary LFs. These data indicate that the CBPs examined may regulate Ca(2+) in the different subcellular sites of FDCs, and the roles of CBPs may be heterogeneous. We also investigated the distribution of caldesmon and S-100 protein in follicular lymphomas on paraffin-embedded tissue sections. FDCs within grades I and II neoplastic follicles clearly expressed caldesmon, but not S-100 protein, except a part of grade II neoplastic follicles. FDCs within grade III follicles showed no caldesmon, but frequently expressed S-100 protein. These results demonstrate that the caldesmon and S-100 protein staining patterns of grade I follicular lymphomas are different from those of grade III follicular lymphomas and suggest that FDC networks in grade I neoplastic follicles may be similar to those in the light zone within non-neoplastic follicles, FDC networks in grade III neoplastic follicles may be similar to those in dark and basal light zones within non-neoplastic follicles, and grade II follicles may be intermediate between grade I and grade III follicles.  (+info)

Affinity labeling of annexin VI with a triazine dye, Cibacron blue 3GA. Probable interaction of the dye with C-terminal nucleotide-binding site within the annexin molecule. (4/118)

Annexin VI (AnxVI) from porcine liver, a member of the annexin family of Ca(2+)- and membrane-binding proteins, has been shown to bind ATP in vitro with a K(d) in the low micromolar concentration range. However, this protein does not contain within its primary structure any ATP-binding consensus motifs found in other nucleotide-binding proteins. In addition, binding of ATP to AnxVI resulted in modulation of AnxVI function, which was accompanied by changes in AnxVI affinity to Ca2+ in the presence of ATP. Using limited proteolytic digestion, purification of protein fragments by affinity chromatography on ATP-agarose, and direct sequencing, the ATP-binding site of AnxVI was located in a C-terminal half of the AnxVI molecule. To further study AnxVI-nucleotide interaction we have employed a functional nucleotide analog, Cibacron blue 3GA (CB3GA), a triazine dye which is commonly used to purify multiple ATP-binding proteins and has been described to modulate their activities. We have observed that AnxVI binds to CB3GA immobilized on agarose in a Ca(2+)-dependent manner. Binding is reversed by EGTA and by ATP and, to a lower extent, by other adenine nucleotides. CB3GA binds to AnxVI also in solution, evoking reversible aggregation of protein molecules, which resembles self-association of AnxVI molecules either in solution or on a membrane surface. Our observations support earlier findings that AnxVI is an ATP-binding protein.  (+info)

Immunological development and cardiovascular function are normal in annexin VI null mutant mice. (5/118)

Annexins are calcium-binding proteins of unknown function but which are implicated in important cellular processes, including anticoagulation, ion flux regulation, calcium homeostasis, and endocytosis. To gain insight into the function of annexin VI, we performed targeted disruption of its gene in mice. Matings between heterozygous mice produced offspring with a normal Mendelian pattern of inheritance, indicating that the loss of annexin VI did not interfere with viability in utero. Mice lacking annexin VI reached sexual maturity at the same age as their normal littermates, and both males and females were fertile. Because of interest in the role of annexin VI in cardiovascular function, we examined heart rate and blood pressure in knockout and wild-type mice and found these to be identical in the two groups. Similarly, the cardiovascular responses of both sets of mice to septic shock were indistinguishable. We also examined components of the immune system and found no differences in thymic, splenic, or bone marrow lymphocyte levels between knockout and wild-type mice. This is the first study of annexin knockout mice, and the lack of a clear phenotype has broad implications for current views of annexin function.  (+info)

Mapping the site of interaction between annexin VI and the p120GAP C2 domain. (6/118)

Annexin VI is a Ca(2+)-dependent membrane and phospholipid binding protein. It mediates a protein-protein interaction with the Ras p21 regulatory protein p120GAP. In this study we have mapped the binding site of GAP within the annexin VI protein. Using Far Western overlay binding assays and cell lysate competition studies we have mapped the site of interaction to the inter-lobe linker region; amino acids 325-363. Finally, using a GST fusion protein corresponding to this linker region we have demonstrated that cellular loading of the fusion protein into Rat-1 fibroblasts by electroporation blocks the interaction and co-immunoprecipitation of annexin VI and GAP.  (+info)

Annexin VI participates in the formation of a reversible, membrane-cytoskeleton complex in smooth muscle cells. (7/118)

The plasmalemma of smooth muscle cells is periodically banded. This arrangement ensures efficient transmission of contractile activity, via the firm, actin-anchoring regions, while the more elastic caveolae-containing "hinge" regions facilitate rapid cellular adaptation to changes in cell length. Since cellular mechanics are undoubtedly regulated by components of the membrane and cytoskeleton, we have investigated the potential role played by annexins (a family of phospholipid- and actin-binding, Ca(2+)-regulated proteins) in regulating sarcolemmal organization. Stimulation of smooth muscle cells elicited a relocation of annexin VI from the cytoplasm to the plasmalemma. In smooth, but not in striated muscle extracts, annexins II and VI coprecipitated with actomyosin and the caveolar fraction of the sarcolemma at elevated Ca(2+) concentrations. Recombination of actomyosin, annexins, and caveolar lipids in the presence of Ca(2+) led to formation of a structured precipitate. Participation of all 3 components was required, indicating that a Ca(2+)-dependent, cytoskeleton-membrane complex had been generated. This association, which occurred at physiological Ca(2+) concentrations, corroborates our biochemical fractionation and immunohistochemical findings and suggests that annexins play a role in regulating sarcolemmal organization during smooth muscle contraction.  (+info)

Annexin VI: an intracellular target for ATP. (8/118)

Annexin VI (AnxVI), an Ca2+- and phospholipid-binding protein, interacts in vitro with ATP in a calcium-dependent manner. Experimental evidence indicates that its nucleotide-binding domain which is localized in the C-terminal half of the protein differs structurally from ATP/GTP-binding motifs found in other nucleotide-binding proteins. The amino-acid residues of AnxVI directly involved in ATP binding have not been yet defined. Binding of ATP to AnxVI induces changes in the secondary and tertiary structures of protein, affecting the affinity of AnxVI for Ca2+ and, in consequence, influencing the Ca2+-dependent activities of AnxVI: binding to F-actin and to membranous phospholipids, and self-association of the annexin molecules. These observations suggest that ATP is a functional ligand for AnxVI in vivo, and ATP-sensitive AnxVI may play the role of a factor coupling vesicular transport and calcium homeostasis to cellular metabolism.  (+info)

*Annexin A6

... is a protein that in humans is encoded by the ANXA6 gene. Annexin VI belongs to a family of calcium-dependent ... "Entrez Gene: ANXA6 annexin A6". "Dysmorphology data for Anxa6". Wellcome Trust Sanger Institute. "Haematology data for Anxa6". ... It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Exon 21 of annexin VI is ... The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of ...

*List of MeSH codes (D12.776.157)

... annexin a4 MeSH D12.776.157.125.050.100 -- annexin a5 MeSH D12.776.157.125.050.110 -- annexin a6 MeSH D12.776.157.125.050.120 ... annexin a1 MeSH D12.776.157.125.050.060 -- annexin a2 MeSH D12.776.157.125.050.070 -- Annexin A3 MeSH D12.776.157.125.050.080 ... annexin a7 MeSH D12.776.157.125.412.249 -- calmodulin MeSH D12.776.157.125.412.311 -- calnexin MeSH D12.776.157.125.412.374 -- ...

*S100A6

S100 calcium-binding protein A6 (S100A6) is a protein that in humans is encoded by the S100A6 gene. The protein encoded by this ... Sudo T, Hidaka H (1999). "Characterization of the calcyclin (S100A6) binding site of annexin XI-A by site-directed mutagenesis ... S100A6 S100 calcium binding protein A6". Deloulme JC, Assard N, Mbele GO, Mangin C, Kuwano R, Baudier J (November 2000). " ...
Annexin A6 (AnxA6) is a Ca2+-dependent membrane-binding protein involved in vesicular traffic. The likely participation of AnxA6 in the response of lymphocytes to Ca2+ signals has not been investigated yet. The present study focuses on intracellular relocation of AnxA6 in human Jurkat T lymphoblasts upon stimulation followed by transient increase of intracellular [Ca2+] and exocytosis of interleukin-2 (IL-2). Stimulation of the cells under different experimental conditions (by lowering pH and/or by rising extracellular [Ca2+] in the presence of ionomycin) induced time-dependent transients of intracellular [Ca2+] and concomitant changes in AnxA6 intracellular localization and in IL-2 secretion, with only minor effects on cell viability and apoptosis. In resting conditions (in the presence of EGTA or with no ionophore) AnxA6 was localized uniformly in the cytosol, whereas it translocated to vesicular structures beneath the plasma membrane within 5 min following stimulation of Jurkat T cells and ...
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis. Alternatively spliced transcript variants have been described. [provided by RefSeq, Aug 2010 ...
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Buy our Recombinant Human Annexin A13 protein. Ab105616 is a full length protein produced in Escherichia coli and has been validated in SDS-PAGE, MS. Abcam…
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Background Good clinical evidence has been reported for the effect of PC6 acupuncture in preventing or attenuating postoperative and pregnancy related nausea. Our primary aim was to examine whether PC6 acupuncture during a period of chemotherapy could complement pharmacological treatment of nausea in cancer patients in the palliative stage of their disease.. Method We conducted a prospective observational pilot study to measure changes in nausea, and also explored the relationship between nausea, pain and constipation. Twelve patients suffering from nausea and four nausea free patients participated in the study. The nausea free patients were included because they had been troubled by nausea in a previous course of chemotherapy, despite medication with antiemetic drugs, and were about to start a new course of treatment. The patients rated their intensity of nausea, pain and constipation on a numerical rating scale before each of 10 treatment sessions with PC6 acupuncture over the course of three ...
The beta-thymosins are a family of highly conserved polar 5 kDa peptides originally thought to be thymic hormones. About 10 years ago, thymosin beta(4) as well as other members of this ubiquitous peptide family were identified as the main intracellular G-actin sequestering peptides, being present in high concentrations in almost every cell. beta-Thymosins bind monomeric actin in a 1:1 complex and act as actin buffers, preventing polymerization into actin filaments but supplying a pool of actin monomers when the cell needs filaments. Changes in the expression of beta-thymosins appear to be related to the differentiation of cells. Increased expression of beta-thymosins or even the synthesis of a beta-thymosin normally not expressed might promote metastasis possibly by increasing mobility of the cells. Thymosin beta(4) is detected outside of cells in blood plasma or in wound fluid. Several biological effects are attributed to thymosin beta(4), oxidized thymosin beta(4), or to the fragment, acSDKP, ...
The role of the calcium- and phospholipid-binding protein annexin I (ANXA1) in cell cycle regulation has been investigated in estrogen receptor (ER)-positive MCF-7 and ER-negative MDA-MB-231 breast tumor cell lines. In MCF-7 cells, ANXA1-targeting small interfering RNA (siRNA) reduced ANXA1 mRNA and protein levels and attenuated cell proliferation induced by FCS, estradiol, or epidermal growth factor. Well-characterized agonists for the known ANXA1 receptor, FPR2, including the ANXA1 N-terminal proteolytic product ANXA1(2-26), lipoxin A(4) (LXA(4)), and the synthetic peptide, Trp-Lys-Tyr-Met-Val-D-Met (WKYMVm), stimulated proliferation of MCF-7 and MDA-MB-231 cells that was attenuated by incubation with FPR2 antagonists WRW(4) (1 μM) or Boc2 (100 nM) or by siRNA against FPR2. FCS-induced mitogenic responses were attenuated by each of the FPR antagonists and by siRNA against FPR2 and, to a lesser extent, FPR1. LXA(4) increased phosphorylation of Akt, p70(S6K) but not ERK1/2. Increases in cyclin ...
Mouse anti Human Annexin A3 antibody, clone 4F1 recognizes human annexin A3, also known as Annexin III, Inositol 1,2-cyclic phosphate 2-ph
ROG has upped the game audio ante with an advanced differential circuit design. It includes an Op-Amp that combines two opposite-phased signals while filtering out noise interference during transmission, providing clear audio stream output with double the intensity. Moreover, SupremeFX on Maximus VI GENE also implements multi-approach shielding, with ELNA audio capacitors helping offer 8-channel HD sound thats equal in power, clarity, and range to dedicated sound cards. This is the first-ever onboard sound capable of true audiophile-grade performance, set to revolutionize the way you hear games, movies, music, and even other people via chat. Discover a whole new dimension of superior audio and get ready to rule. Click the button to learn more about SupremeFX!. ...
1HVE: Structural and electrophysiological analysis of annexin V mutants. Mutagenesis of human annexin V, an in vitro voltage-gated calcium channel, provides information about the structural features of the ion pathway, the voltage sensor and the ion selectivity filter
Heroes VI is a fast-paced epic story where Angels plot to end -- once and for all -- an unfinished war with their ancient rivals, the Faceless.
CHAPTER VI IMPACT OF PRIORITY SECTOR LENDING 6.1 PRINCIPAL FACTORS THAT HAVE DIRECT IMPACT ON PRIORITY SECTOR LENDING 6.2 ASSOCIATION BETWEEN THE PROFILE VARIABLES AND IMPACT OF PRIORITY SECTOR CREDIT
Anexina A3 é uma proteína que nos humanos é codificada pela gene ANXA3. É expressada de maneira anormal em fetos derivados de fertilização in vitro e de microinjecção intracitoplasmática, podendo contribuir para um risco aumentado de defeitos no recém-nascido nestas tecnologias de reprodução medicamente assistida. Este gene codifica um membro da família das anexinas. Os membros desta família de proteínas ligadas aos fosfolipídios dependentes de cálcio desempenham um papel na regulação do crescimento celular e em vias de transdução de sinal. Esta proteína funciona na inibição da fosfolipase A2 e na clivagem de inositol 1,2-fosfato cíclico para formar inositol 1-fosfato. Também desempenha um papel na anti-coagulação. Tait JF, Frankenberry DA, Miao CH, Killary AM, Adler DA, Disteche CM (agosto de 1991). «Chromosomal localization of the human annexin III (ANX3) gene». Genomics. 10 (2): 441-8. PMID 1830024. doi:10.1016/0888-7543(91)90330-H !CS1 manut: Nomes múltiplos: ...
Mouse Monoclonal Anti-ANXA1 Antibody against Human annexin A1. Validated for Immunofluorescence, Immunohistochemistry and Western Blot
In our hands, the trypsin actually reduced the Annexin staining presumably by stripping off all the phosphotidyl-serine on the surface along with the surface proteins (adhesion molecules). The only time we have seen enhanced annexin is when the cells suffer enough trauma in harvesting that the membrane is not intact and the Annexin gets inside the cell and attaches to the ps on the inner membrane. Julie At 02:11 PM 8/18/98, you wrote: , ,I have a client who is staining with Annexin-FITC. They are looking at ,cell lines and are using trypsin to get the cells off the flasks. We are ,seeing what we think is an exagerated Annexin response. Does anyone out ,there have any feedback on how trypsin affects Annexin staining? Any ,experience and or information about this will be greatly appreciated. , Thanks in advance, , Joan Kalnitsky ,Joan Kalnitsky ,Flow Cytometry Laboratory ,VMRCVM ,(540) 231-4115 ,FAX 540-231-7367 , , It is better to serve than to receive , B. Borg , , , , , ...
Modified annexin proteins, including a homodimer of human annexin V, are provided. Methods for their use, such as to prevent thrombosis without increasing hemorrhage, enhancing the survivability of platelets during storage or transfusion and to attenuate ischemia-reperfusion injury (IPI), are also provided. The modified annexins bind phosphatidylserine (PS) on cell surfaces, thereby preventing the assembly of the prothrombinase complex. The modified annexin decreases the binding of leukocytes and platelets during post-ischemic reperfusion, thereby restoring microvascular blood flow and decreasing organ damage. In addition, the modified annexin prevents lipid loss from platelets during storage.
1HVD: Structural and electrophysiological analysis of annexin V mutants. Mutagenesis of human annexin V, an in vitro voltage-gated calcium channel, provides information about the structural features of the ion pathway, the voltage sensor and the ion selectivity filter
Annexin A5 is a biomarker used in Hiv Infection, Acute Lymphoblastic Leukemia, Hypertension and 942 other diseases. Learn more about Annexin A5.
ATP- and membrane-binding protein that probably controls membrane reorganization/tubulation upon ATP hydrolysis. Plays a role in early endosomal transport.
ATP- and membrane-binding protein that probably controls membrane reorganization/tubulation upon ATP hydrolysis. Plays a role in early endosomal transport.
Annexin A1 Antibody 66344-1-Ig has been identified with ELISA, IF, IHC, WB. 66344-1-Ig detected 35 kDa band in A431 cells with 1:2500-1:10000 dilution...
Annexin A11 Antibody 10479-2-AP has been identified with IF, IHC, IP, WB, ELISA. 10479-2-AP detected 56-60 kDa band in HeLa cells with 1:500-1:2000 dilution...
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Annexin A1 is an intracellular calcium/phospholipid-binding protein that is involved in membrane organization and the regulation of the immune system. It has been attributed an anti-inflammatory role at various control levels, and recently we could show that annexin A1 externalization during secondary necrosis provides an important fail-safe mechanism counteracting inflammatory responses when the timely clearance of apoptotic cells has failed. As such, annexin A1 promotes the engulfment of dying cells and dampens the postphagocytic production of proinflammatory cytokines. In our current follow-up study, we report that exposure of annexin A1 during secondary necrosis coincided with proteolytic processing within its unique N-terminal domain by ADAM10. Most importantly, we demonstrate that the released peptide and culture supernatants of secondary necrotic, annexin A1-externalizing cells induced chemoattraction of monocytes, which was clearly reduced in annexin A1- or ADAM10-knockdown cells. Thus, ...
Annexin A2 is a calcium-dependent, phospholipid-binding protein found on many cell types. It consists of a short hydrophobic tail (Ser2-Asn32), which dictates its function, and a core domain (Phe33-Asp339), which is involved in phospholipid binding. Annexin A2 has been implicated in a number of biochemical processes, including cell proliferation, foetal immune tolerance, ion-channel activation, cell-cell interactions and the bridging of membranes. Annexin A2 is reported to be a powerful activator of plasminogen and, therefore, is implicated in many normal and pathological processes such as haemostasis and metastasis. Myeloid cell lines are used, extensively, to study many aspects of cellular proliferation, differentiation and function. In the present study, we have used flow cytometry and real-time PCR to investigate the role of annexin A2 expression in the proliferation and differentiation of a number of myeloid cell lines. The results demonstrated that annexin A2 expression was affected when ...
The anti-inflammatory protein annexin 1 may protect patients from the ...Severe inflammatory response syndrome or SIRS occurs when the bodys...Damazo et al. studied this balancing act by analyzing the effects of t...When normal mice were treated with LPS the immune system induced a st...The researchers characterized the expression of annexin 1 and found th...,Protein,prevents,detrimental,immune,effects,of,bacterial,sepsis,biological,biology news articles,biology news today,latest biology news,current biology news,biology newsletters
Copines make up a family of soluble, calcium-dependent membrane binding proteins found in a wide variety of eukaryotic organisms. Copines are characterized as having two C2 domains at the N-terminal region followed by an "A domain," similar to the von Willebrand A (VWA) domain found in integrins, in the C-terminal region [1]. Copines appear to be absent from the Sacchromyces cerevisae genome, while the genomes of Paramecium, Arabidopsis, C. elegans, and human have two, three, five, and nine, respectively [13, 4, 24]. Tomsig and Creutz [8] have hypothesized that copines play a role in calcium signaling by binding to target proteins with their A domains and then bringing those target proteins to a particular membrane through the action of their C2 domains in response to a rise in calcium concentration. Because copines have two C2 domains, they have also been hypothesized to have a role in membrane trafficking. It is possible that both ideas could be correct; copines may provide links between ...
The Annexin family of calcium-binding proteins is composed of al least thirteen mammalian genes (Annexin A1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. The Annexin family
|p|Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry
BioAssay record AID 590043 submitted by ChEMBL: Induction of apoptosis in human K562 cells assessed as viable cells at 50 nM using annexin V-propidium iodide and annexin V staining by flow cytometry (Rvb = 87.02%).
Buy ANXA2 recombinant protein, Annexin A2 Recombinant Protein-NP_001002857 (MBS203687) product datasheet at MyBioSource, Recombinant Proteins. Application: SDS-PAGE
Bachem offers H-3196 Annexin A1 (1-25) (dephosphorylated) (human) for your research. Find all specific details here. Find product specific information including available pack sizes, CAS, detailed description and references here.
Complete information for ANXA1 gene (Protein Coding), Annexin A1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Complete information for ANXA1 gene (Protein Coding), Annexin A1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
www.ncbi.nlm.nih.gov/pmc/articles/PMC3063939. Our results indicate that 4-1BB co-stimulation may significantly improve TIL survival during ... mAb from Bristol Myers Squibb (BMS) currently being tested in clinical trials,. ... BMS-663513 was at 14.9 mg/ml and was stored at 4°C. This Ab was .... The cells were then stained with anti-CD8-Pacific Blue, Annexin V-PE, and .... ...
Membrane functionalization is a promising strategy for augmenting cell performance in regenerative medicine. To this end, the design, construction, characterisation and cell affinity of protein-polymer surfactant nanoconstructs are presented. Nanoconstructs of eGFP were synthesised that exhibited near-native structure and function, as well as effective and persistent membrane affinity. Human mesenchymal stem cells were labelled for up to ten days in culture, without affecting cell viability or differentiation capacity. This "cell priming" technology has been used to address the issue of hypoxia-related central necrosis during in-vitro tissue engineering. Specifically, nanoconstructs of myoglobin, with enhanced oxygen-binding affinity, were synthesised and used to prime mesenchymal stem cells prior to hyaline cartilage engineering. The myoglobin-primed cells produced tissue constructs with a 62 % increase in type II : type I collagen ratio and, significantly, a reduction in cell necrosis from 42 ...
Annexin V Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidyl-serine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. The one-step staining procedure takes only 10 minutes. Detection can be analyzed by flow cytometry or by fluorescence microscopy. |p>|br />|br />‧ Detection method- Flow cytometry (Ex = 543 nm; Em = 570 nm) and fluorescence microscopy |br>|br />‧ Sample type- Living cells (suspension and adherant)|br>|br />‧ Species reactivity- Mammalian |br />|br />|br />|p>|b>Features and Benefits|/b>|br>|br />‧ Simple one step staining procedure in 10 minutes|br>|br />‧ Fast and convenient|br>|br />‧ Cy3 shows brighter red fluorescence|br />|br />|br />|br />|p>|b>Kit Contents:|/b>|br>|br />Annexin V-Cy3|br>|br />1X Binding Buffer, Annexin V
TransDetect® Annexin V-EGFP/PI Cell Apoptosis Detection Kit,Cell Detection,Cell Culture and Detection,Products,Beijing TransGen Biotech Co.Ltd,OverviewContents& storageCitations & referencesRelated ImagesDownloadOverviewDescriptionThe Annexin V
Annexin A1 / ANXA1, 0.1 ml. Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane.
Annexin V is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Annexin V is a monomer, binds ATRX and EIF5B, and interacts with hepatitis B virus (HBV ...
During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca2+-dependent affinity for PS and therefore is used as a probe for detecting apoptosis., Annexin V-FITC Apoptosis Detection Reagent, GTX14082, Applications: FACS; Flow cytometry/FACS; CrossReactivity:
Many eukaryotic cells divide by assembling and constricting an actin- and myosin-based contractile ring (CR) that is physically linked to the plasma membrane (PM). In this study, we report that Schizosaccharomyces pombe cells lacking efr3, which encodes a conserved PM scaffold for the phosphatidylinositol-4 kinase Stt4, build CRs that can slide away from the cell middle during anaphase in a myosin V-dependent manner. The Efr3-dependent CR-anchoring mechanism is distinct from previously reported pathways dependent on the Fes/CIP4 homology Bin-Amphiphysin-Rvs167 (F-BAR) protein Cdc15 and paxillin Pxl1. In efr3Δ, the concentrations of several membrane-binding proteins were reduced in the CR and/or on the PM. Our results suggest that proper PM lipid composition is important to stabilize the central position of the CR and resist myosin V-based forces to promote the fidelity of cell division. ...
Many eukaryotic cells divide by assembling and constricting an actin- and myosin-based contractile ring (CR) that is physically linked to the plasma membrane (PM). In this study, we report that Schizosaccharomyces pombe cells lacking efr3, which encodes a conserved PM scaffold for the phosphatidylinositol-4 kinase Stt4, build CRs that can slide away from the cell middle during anaphase in a myosin V-dependent manner. The Efr3-dependent CR-anchoring mechanism is distinct from previously reported pathways dependent on the Fes/CIP4 homology Bin-Amphiphysin-Rvs167 (F-BAR) protein Cdc15 and paxillin Pxl1. In efr3Δ, the concentrations of several membrane-binding proteins were reduced in the CR and/or on the PM. Our results suggest that proper PM lipid composition is important to stabilize the central position of the CR and resist myosin V-based forces to promote the fidelity of cell division. ...
SCAN HISTORY OWL11_0 2 100 NSINGLE OWL17_1 2 110 NSINGLE OWL18_0 1 115 NSINGLE OWL19_1 1 125 NSINGLE OWL21_1 1 125 NSINGLE OWL26_0 1 125 NSINGLE SPTR37_9f 2 237 NSINGLE INITIAL MOTIF SETS ANNEXIN1 Length of motif = 23 Motif number = 1 Annexin motif I - 1 PCODE ST INT KTKGVDEVTIVNILTNRSNAQRQ LUHU36 46 46 KTKGVDEVTIINILTNRSNEQRQ ANX2_CHICK 46 46 TVKGVDEATIIDILTKRNNAQRQ ANX1_CAVCU 56 56 MVKGVDEATIIDILTKRNNAQRQ LUHU 55 55 KGIGTDEATIIDIVTHRSNAQRQ ANX6_MOUSE 376 376 KGMGTDEETILKILTSRNNAQRQ ANX5_CHICK 29 29 KGIGTNEQAIIDVLTKRSNTQRQ ANX8_HUMAN 35 35 KGFGTDEQEIIDVLVGRSNQQRQ DROANNX 29 29 RGIGTDEKMLISILTERSNAQRQ ANX3_HUMAN 32 32 KGLGTDEDAIINVLAYRSTAQRQ ANX4_BOVIN 27 27 KGFGTDEQAIVDVVANRSNDQRQ HUMSNEXIN 177 177 TAKGVDEATIIDIMTTRTNAQRP ANX1_COLLI 51 51 ANNEXIN2 Length of motif = 17 Motif number = 2 Annexin motif II - 1 PCODE ST INT LKSALSGHLETVILGLL LUHU36 86 17 LKSALSGHLEAVILGLL ANX2_CHICK 86 17 LKKALTGHLEEVVLALL ANX1_CAVCU 96 17 LKKALTGHLEEVVLALL LUHU 95 17 LKSEISGDLARLILGLM ANX6_MOUSE 416 17 ...
Page contains details about annexin V conjugated to maleimide-functionalized Feraheme nanoparticles . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
BioAssay record AID 502910 submitted by ChEMBL: Induction of annexin 2-mediated cytoskeleton aggregate accumulation in human HepG2 cells at 4 uM after 2 hrs by confocal laser scanning microscopy.
Phospholipid-binding protein EhC2A mediates calcium-dependent translocation of transcription factor URE3-BP to the plasma membrane of Entamoeba histolytica ...
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Annexin A2 is a calcium regulated protein involved in membrane binding and actin polymerization. In this paper, Kevin Moreau and colleagues identified Annexin A2 as a…. ...
Sigma-Aldrich offers abstracts and full-text articles by [Jiangnan Li, Dongwei Guo, Li Huang, Manman Yin, Qingfang Liu, Yan Wang, Chunmei Yang, Yuanyuan Liu, Lijie Zhang, Zhijun Tian, Xuehui Cai, Liyun Yu, Changjiang Weng].
It has been reported that Annexin A2 (ANXA2) is up-regulated in hepatocellular carcinoma (HCC), but the roles of ANXA2 in the migration and invasion of HCC ...
Annexin A1, also known as lipocortin I, is a protein that is encoded by the ANXA1 gene in humans. Annexin A1 belongs to the annexin family of Ca2+-dependent phospholipid-binding proteins that have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin A1 protein has an apparent relative molecular mass of 40 kDa with phospholipase A2 inhibitory activity. Glucocorticoids (such as budesonide, cortisol, and beclomethasone) are a class of endogenous or synthetic anti-inflammatory steroid hormones that bind to the glucocorticoid receptor (GR), which is present in almost every vertebrate animal cell. They are used in medicine to treat diseases caused by an overactive immune system, including allergies, asthma, autoimmune diseases, and sepsis. Because they suppress inflammatory pathways, long-term use of glucocorticoid drugs can lead to side-effects such as immunodeficiency and adrenal insufficiency. The main mechanism of ...
TY - JOUR. T1 - Annexins family. T2 - Insights into their functions and potential role in pathogenesis of sarcoidosis. AU - Mirsaeidi, Mehdi. AU - Gidfar, Sanaz. AU - Vu, Ann. AU - Schraufnagel, Dean. PY - 2016. Y1 - 2016. N2 - Annexins are Ca2+-regulated phospholipid-binding proteins that play an important role in the cell life cycle, exocytosis, and apoptosis. Annexin A11 is one of the oldest vertebrate annexins that has a crucial role in sarcoidosis pathogenesis. The mechanism of effect in sarcoidosis granuloma cells may be due to alterations in apoptosis. Immune cells with a specific mutation at protein location 230 are resistant to apoptosis and consequently have continued effects on inflammation and progression of sarcoidosis. The mechanism of action of annexin A11 may be based upon alterations in delivering calcium to two different apoptosis pathways (caspase and P53).. AB - Annexins are Ca2+-regulated phospholipid-binding proteins that play an important role in the cell life cycle, ...
Purpose: : To examine the role of annexin 2 in the formation and internalisation of phagosomes in retinal pigment epithelial (RPE) cells. Methods: : Human ARPE19 cells were cultured on the glass inserts of Matek dishes for immunofluorescence and live imaging, or plastic Nunc dishes for biochemical analysis. In some studies ARPE19 cells were exposed to siRNA for annexin 2 for three days prior to experimentation. Outer segments were prepared from porcine retina using standard protocols (typically 50 - 100 eyes per prep), and either used fresh in biochemical experiments, or labelled with an appropriate fluorescent marker prior to imaging experiments. Results: : Depletion of annexin 2 using siRNA led to a significant quantitative reduction in the phagocytosis of rod outer segments in cultured RPE cells. In cells expressing annexin 2, imaged by confocal microscopy, a striking enrichment of annexin 2 and F-actin was observed at the phagocytic cup immediately upon engagement of the apical cell surface ...
The discovery of an essential role for annexin 11 in cell division is unexpected when considered in the broader context of annexin biology. Although annexins are now firmly implicated in various cellular activities, including endocytosis, calcium signaling, and apoptosis, no member of this protein family has been demonstrated to function in the cell cycle. The membrane binding and vesicle fusogenic properties that define annexins in biochemical terms could provide clues as to the possible role of annexin 11 in the last stage of cytokinesis. Before cells can be completely separated in a process known as abscission, the actomyosin constriction ring at the cleavage furrow has to be disassembled and the central spindle must separate. During this series of events, it is likely that the plasma membrane must be tightly attached to the midbody microtubules for abscission to be successfully completed. CHO1 has previously been suggested to be responsible for this connection (Kuriyama et al., 2002), but ...
Membrane repair is essential to cell survival. In skeletal muscle, injury often associates with plasma membrane disruption. Additionally, muscular dystrophy is linked to mutations in genes that produce fragile membranes or reduce membrane repair. Methods to enhance repair and reduce susceptibility to injury could benefit muscle in both acute and chronic injury settings. Annexins are a family of membrane-associated Ca2+-binding proteins implicated in repair, and annexin A6 was previously identified as a genetic modifier of muscle injury and disease. Annexin A6 forms the repair cap over the site of membrane disruption. To elucidate how annexins facilitate repair, we visualized annexin cap formation during injury. We found that annexin cap size positively correlated with increasing Ca2+ concentrations. We also found that annexin overexpression promoted external blebs enriched in Ca2+ and correlated with a reduction of intracellular Ca2+ at the injury site. Annexin A6 overexpression reduced membrane ...
Helen Christians research interests are in the mechanisms of steroid hormone regulation of the pituitary gland, in particular the role of Annexin 1. The Annexins are a well-conserved super-family of structurally related Ca2+ - and phospholipids-binding proteins with wide-ranging functions in health and disease. Annexin 1 is a 37kD protein that is induced by glucocorticoids and mediates glucocorticoid action within the host defence and neuroendocrine systems. Glucocorticoids regulate the synthesis, phosphorylation and cellular disposition of annexin 1, and annexin 1 is implicated in the regulation by these important drugs of pituitary function, the control of cell growth and signal transduction. Most recently her group have characterized a novel secretory pathway of annexin 1, a protein which lacks a signal sequence, involving a member of the ATP binding cassette transporter family, ABCA1. This is the first time the secretion mechanism of an annexin family member has been determined and the ...
In the present study, we show that both CRP and annexin A5 specifically bind to oxidized LDL. Via coupling to Fcγ receptors on macrophages and independent of complement, CRP subsequently enhances the association of oxidized LDL to macrophages. Enhanced association of oxidized LDL to macrophages via CRP-Fcγ receptor interaction may lead to enhanced macrophage uptake of oxidized LDL. Moreover, we show that annexin A5 binds to a site at oxidized LDL different from the CRP binding site and that annexin A5 does not antagonize the CRP effect.. Previously, Chang et al also observed specific binding of CRP to oxidized LDL,7 characterized by inhibition by whole molecules as well as F(ab′)2 fragments of EO6, an IgM antibody that binds specifically to the phosphorylcholine head group of oxidized phospholipids but not to the same moiety on nonoxidized phospholipids.26 In the present study, for characterization of binding sites, we used the monoclonal antibody DLH3 (also IgM), which recognizes an epitope ...
ANXA2, a member of the annexin family, is a calcium-dependent phospholipid-binding protein that plays a role in the regulation of cellular growth and in signal transduction pathways. Its affinity for calcium is greatly enhanced by anionic phospholipids, and it binds two calcium ions with high affinity. ANXA2 may be involved in heat-stress response. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. It may also cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis.
References for Abcams Annexin V-PE-Cy5 Apoptosis Staining / Detection Kit (ab14159). Please let us know if you have used this product in your publication
Tinción con anexina V y propidio yoduro (PI) ofrece a los investigadores una manera de identificar distintos tipos de muerte celular:...
Mouse Monoclonal Anti-Annexin A3 Antibody (2F3). Validated: WB, ICC/IF, IHC, IHC-P. Tested Reactivity: Human, Monkey. 100% Guaranteed.
The surface of healthy cells is composed of lipids that are asymmetrically distributed on the inner and outer leaflet of the plasma membrane. One of these lipids, phosphatidylserine (PS), is normally restricted to the inner leaflet of the plasma membrane and is, therefore, only exposed to the cell cytoplasm. However, during apoptosis lipid asymmetry is lost and PS becomes exposed on the outer leaflet of the plasma membrane. Annexin V, a 36-kDa calcium-binding protein, binds to PS; therefore, fluorescently labeled Annexin V can be used to detect PS that is exposed on the outside of apoptotic cells. Annexin V can also stain necrotic cells because these cells have ruptured membranes that permit Annexin V to access the entire plasma membrane. However, apoptotic cells can be distinguished from necrotic cells by co-staining with propidium iodide (PI) because PI enters necrotic cells but is excluded from apoptotic cells. This protocol describes Annexin V binding and PI uptake followed by flow cytometry ...
For those of you who were following the Caspase-3 thread and those of you who contacted me directly, heres the information regarding Caspase-3 and Annexin V that I received from Pharmingen. Any furthur questions, please contact Padma at Pharmingen. Padma was most helpful. Lora , -----Original Message----- , From: pkodukula at pharmingen.com [SMTP:pkodukula at pharmingen.com] , Sent: Monday, October 04, 1999 10:14 AM , To: Barsky, Lora , Subject: RE: caspase-3 , , , , Dear Lora, , , Thank you for your interest in PharMingens products. This is in reply to , your , question about the caspase 3 and the annexin V staining. We are releasing , a kit , for the BrDU and cytokine staining. But the annexin V is also known to , work , with the caspase 3. This is not something that we routinely quality , control in , our company but we have had input from customers who have used this , method. The , annexin V binding to phosphatidyl serine is dependent on the CA++ ion , concentration and so a Ca++ ...
Annexin V-FITC Apoptosis assay results: Flow cytometry profiles represent Annexin-V-FITC staining in X-axis and PI in Y-axis: (a) control, i.e., neither PSi nor
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Renshaw, Derek, Montero-Melendez, Trinidad, Dalli, Jesmond, Kamal, Ahmad, Brancaleone, Vincenzo, DAcquisto, Fulvio, Cirino, Giuseppe and Perretti, Mauro (2010) Downstream gene activation of the receptor ALX by the agonist Annexin A1. PLoS ONE, 5 (9). e12771. ISSN 1932-6203 ...
АДСОРБЦІЯ ІОНІВ Cr (VI) ТА Co (II) ПАЛИГОРСЬКІТОМ, МОДИФІКОВАНИМ КАТІОННИМИ ПОВЕРХНЕВО- АКТИВНИМИ РЕЧОВИНАМИ
Gliomas are highly invasive brain tumors with the occurrence of numerous microglia/macrophages (MG/MP) in and around the tumor. Annexin A2 is overexpressed in many cancers and correlates with increased plasmin activity on the tumor cell surface. Plasmin mediates degradation of extracellular matrix and angiogenesis to facilitate tumor growth. In this study, we used a mouse glioma cell line, GL261-EGFP, and stable clones transfected with an annexin A2 knockdown (annA2KD) construct, GL261-EGFP-annA2KD. We found that the annA2KD decreased glioma cell migration in vitro and decreased membrane-bound plasmin activity. In vivo we injected GL261-EGFP cells into the mouse brain and the glioma progression was followed. Knockdown of annexin A2 in glioma cells decreased tumor size and slowed down tumor progression, characterized by decreased invasion, angiogenesis and proliferation, as well as increased apoptosis in tumor tissue of the annA2KD group. We also investigated the interaction between glioma and ...
TY - JOUR. T1 - Assessment of secondary necrosis of Jurkat cells using a new microscopic system and double staining method with annexin V and propidium iodide.. AU - Honda, O.. AU - Kuroda, Masahiro. AU - Joja, I.. AU - Asaumi, Jun-Ichi. AU - Takeda, Yoshihiro. AU - Akaki, S.. AU - Togami, I.. AU - Kanazawa, Susumu. AU - Kawasaki, S.. AU - Hiraki, Y.. PY - 2000/2. Y1 - 2000/2. N2 - Using a new system developed by us for acquiring microscopic images automatically, we compared the morphological changes that apoptotic cells undergo with changes in the staining pattern of annexin V-enhanced green fluorescent protein (AV-EGFP) and propidium iodide (PI) in individual cells. Jurkat cells were treated with 5 mM CaCl2 alone, anti-Fas antibody and heating at 42 degrees C for 30 min or 46 degrees C for 60 min, and then were incubated in medium with 5 mM CaCl2. Time-lapse DNA fragmentation analysis and morphological observation revealed that the anti-Fas antibody and heating at 42 degrees C for 30 min ...
Annexxin of 369 aas. Schistosomiasis, a major parasitic disease of humans, is second only to malaria in its global impact. The disease is caused by digenean trematodes that infest the vasculature of their human hosts. These flukes are limited externally by a body wall composed of a syncytial epithelium, the apical surface membrane, a parasitism-adapted dual membrane complex. Annexins are important for the stability of this apical membrane system. Leow et al. 2013 presented the first structural and immunobiochemical characterization of an annexin from Schistosoma mansoni. The crystal structures of annexin B22 (4MDV and 4MDU) in the apo and Ca2+ bound forms confirmed the presence of the previously predicted α-helical segment in the II/III linker and revealed a covalently linked head-to-head dimer. The dimeric arrangement revealed a non-canonical membrane binding site and a probable binding groove opposite the binding site. Annexin B22 expression correlated with life stages of the parasite that ...
The pHi (intracellular pH) is an important physiological parameter which is altered during hypoxia and ischaemia, pathological conditions accompanied by a dramatic decrease in pHi. Sensors of pHi include ion transport systems which control intracellular Ca2+ gradients and link changes in pHi to functions as diverse as proliferation and apoptosis. The annexins are a protein family characterized by Ca2+-dependent interactions with cellular membranes. Additionally, in vitro evidence points to the existence of pH-dependent, Ca2+-independent membrane association of several annexins. We show that hypoxia promotes the interaction of the recombinant annexin A2-S100A10 (p11) and annexin A6 with the plasma membrane. We have investigated in vivo the influence of the pHi on the membrane association of human annexins A1, A2, A4, A5 and A6 tagged with fluorescent proteins, and characterized this interaction for endogenous annexins present in smooth muscle and HEK (human embryonic kidney)-293 cells ...
AAD positive means late apoptosis? - posted in Flow Cytometry: We have a discussion in the lab about apoptosis detection by annexin V/AAD labeling. Many people say that Annexin V+/AAD+ cells must not be considered apoptotic but necrotic. I agree, provided that the apoptotic stimulus was rather short (for example 2 hours-staurosporine). However, when the stimulus lasts for 24 hours (serum starvation for instance) I think that cells which started the apoptotic process shortly after the start...
Sigma-Aldrich offers abstracts and full-text articles by [Jakob Voelkl, Ioana Alesutan, Tatsiana Pakladok, Robert Viereck, Martina Feger, Sobuj Mia, Tanja Schönberger, Angelika A Noegel, Meinrad Gawaz, Florian Lang].
Purified Recombinant Human ANXA2 293 Cell Lysate from Creative Biomart. Recombinant Human ANXA2 293 Cell Lysate can be used for research.
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Nectria haematococca mating population (MP) VI isolates that contain the MAK1 gene are able to degrade maackiain, a chickpea (Cicer arietinum) phytoalexin, to a less toxic compound. To test the contribution of MAK1 to the virulence of N. haematococca MP VI on chickpea, the MAK1 gene was disrupted in a highly virulent Mak+ isolate or added to a weakly virulent Mak- isolate via transformation. The disruption of MAK1 decreased virulence to a moderate level, while addition of multiple copies of MAK1 increased virulence to either a moderate or a high level. These data demonstrate that maackiain detoxification is a determinant, but not the only determinant, of virulence in N. haematococca MP VI isolates capable of causing disease on chickpea. MAK1 is located on a 1.6-Mb conditionally dispensable chromosome. To ascertain if there are additional genes influencing virulence toward chickpea stems on the MAK1 chromosome, the loss of this chromosome was chemically induced in an isolate containing the ...
Annexin A1 (ANXA1) is a calcium- and phospholipid-binding protein and a known mediator of glucocorticoid-regulated inflammatory responses. Using a combined multiple high-throughput approach, we recently identified a reduced expression of ANXA1 in human breast cancer. The finding was confirmed at the gene level by quantitative reverse transcription-polymerase chain reaction and at the protein level by immunohistochemical staining of normal, benign, and malignant breast tissues. In this study, we constructed and used a high-density human breast cancer tissue microarray to characterize the expressional pattern of ANXA1 according to histopathologies. The tissue microarray contains 1,158 informative breast tissue cores of different histologies including normal tissues, hyperplasia, in situ and invasive tumors, and lymph node metastases. Our results showed that there was a significant decrease in glandular expression of ANXA1 in ductal carcinoma in situ and invasive ductal carcinoma compared with ...
Proteolytic cleavage of haemagglutinin (HA) is essential for the infectivity of influenza A viruses (IAVs). This is usually mediated by trypsin-like proteases present in the respiratory tract. However, the ability to use plasminogen (PLG) as an alternative protease may contribute to pathogenesis of IAV infections and virus replication outside the respiratory tract. It was demonstrated previously that neuraminidase (NA) of the IAV strain A/WSN/33 can sequester PLG, allowing this virus to replicate in a PLG-dependent fashion. However, PLG also promotes replication of other IAVs, although its mode of action is poorly understood. Here, using NA-deficient viruses, we demonstrate that NA is not required for the binding of PLG and subsequent cleavage of HA. However, we demonstrate that the cellular protein annexin 2 (A2) can bind PLG and contributes to PLG-dependent cleavage of HA and subsequent IAV replication. Collectively, these results indicate that PLG promotes IAV replication in an A2-dependent fashion
Based on sequence information from tryptic peptides an almost full-size cDNA coding for the human vascular anticoagulant was isolated from a placental cDNA library and sequenced. The coding region was cloned into an Escherichia coli expression vector and the protein expressed at high levels. The recombinant protein was purified and found to be indistinguishable from its natural counterpart in several biological assays ...
Annexin A6 (AnxA6) has been implicated in the regulation of endo-/exocytic pathways, cholesterol transport, and the formation of multifactorial signaling complexes in many different cell types. More recently, AnxA6 has also been linked to triglyceride storage in adipocytes. Here we investigated the potential role of AnxA6 in fatty acid (FA) induced lipid droplet (LD) formation in hepatocytes. AnxA6 was associated with LD from rat liver and HuH7 hepatocytes. In oleic acid (OA) -loaded HuH7 cells, substantial amounts of AnxA6 bound to LD in a Ca2+-independent manner. Remarkably, stable or transient AnxA6 overexpression in HuH7 cells led to elevated LD numbers/size and neutral lipid staining under control conditions as well as after OA loading compared to controls. In contrast, overexpression of AnxAl, AnxA2 and AnxA8 did not impact on OA-induced lipid accumulation. On the other hand, incubation of AnxA6-depleted HuH7 cells or primary hepatocytes from AnxA6 KO-mice with OA led to reduced FA ...
Annexin A2 (AnxA2) is a multi-functional and -compartmental protein whose subcellular localisation and functions are tightly regulated by its post-translational modifications. AnxA2 and its Tyr23-phosphorylated form (pTyr23AnxA2) are involved in malignant cell transformation, metastasis and angiogenesis. Here, we show that H2O2 exerts rapid, simultaneous and opposite effects on the Tyr23 phosphorylation status of AnxA2 in two distinct compartments of rat pheochromocytoma (PC12) cells. Reactive oxygen species induce dephosphorylation of pTyr23AnxA2 located in the PML bodies of the nucleus, whereas AnxA2 associated with F-actin at the cell cortex is Tyr23 phosphorylated. The H2O2-induced responses in both compartments are transient and the pTyr23AnxA2 accumulating at the cell cortex is subsequently incorporated into vesicles and then released to the extracellular space. Blocking nuclear export by leptomycin B does not affect the nuclear pool of pTyr23AnxA2, but increases the amount of total AnxA2 ...
Antiphospholipid syndrome is associated with an increased risk of thrombosis and pregnancy loss. Annexin A5 (Anxa5) is a candidate autoantigen. It is not known, however, whether endogenous Anxa5 prevents foetal loss during normal pregnancy. We found
Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 has 45 to 59% identity with other members of its family and shares a similar size and exon-intron organization. Isolated from human placenta, ANX4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells ...
Auranofin promotes mitochondrial apoptosis by inducing annexin A5 expression and translocation in human prostate cancer cells.: Auranofin is a lipophilic gold c
Frodariok I). Hill Uilo "t itri ):-r-)i City IS Organizations Seeking Law With Teeth nr r"r"3Tn n rrmrN cnn la F uwinJU an ifu r 1 ANIPy FIRST Vol. 18 No. 31 COLUMBUS, OHIO SATURDAY, AUGUST 2, 1952 Price 10c filter1 :(f&f$A , If -: "f 1 ;i j "Yy-tj,. ,! . 1.. i V-TinimuMi THEY SPEAK SUNDAY: Jack Kroll. left, national di-lector of the ClOt Political Action Committee, and Illinois ConftreMtnan William L. Dawson, right, of Chicago, are scheduled as the principal spakers Sunday, August 3, at the Big Walnut Country Club when the Jeffersonian Democratic Club of Columbus hosts the third annual State Democratic Connfab. Others slated to appear on the program are Joseph E. Bowman, delegate to the Democratic Natioaal Convention; Mrs. Esther Archer, Canton councilwoman; and Harland Randolph, Ohio State University debate team captain. Free bus transportation will be provided for those calling the office of Frank C. Shearer, Jeffersonian Club president. Opinion !s Divided On i -"-vi On The Mional ...
Pope Paul VI on July 25, 1968 and addressed "to all men of good will.". Cardinal Carter, then Bishop of London, describes the reaction of the bishops: "We promptly dropped everything else we were doing and pored over the encyclical. It was with a certain sense of dismay that we read vital passages n it. He (the Pope) had clearly taken a position that was contrary to the majority position of his own Commission. We felt that this was going to be a major problem.". 6. Archbishop Plourde of Ottawa. In August of 1968, Archbishop Plourde of Ottawa issued a pastoral letter on Humanae Vitae. He said that individuals have the right to reach a judgement different from that of the Holy Father.. 7. The Mandate of the Bishops. The Canadian bishops had a triple mandate at Winnipeg.. As individuals they had the personal mandate shared by all Catholics of giving internal and external assent to the doctrine of Humanae Vitae.. As bishops they had an obligation to follow the pastoral norms given specifically to ...
Summary of ANXA7 expression in human tissue. Ubiquitous cytoplasmic and membrane expression, including expression in pancreatic islets.

Annexins sense changes in intracellular pH during hypoxia | Biochemical JournalAnnexins sense changes in intracellular pH during hypoxia | Biochemical Journal

Our results show that annexin A6 and the heterotetramer A2-S100A10 (but not annexins A1, A4 and A5) interact independently of ... We show that hypoxia promotes the interaction of the recombinant annexin A2-S100A10 (p11) and annexin A6 with the plasma ... We have investigated in vivo the influence of the pHi on the membrane association of human annexins A1, A2, A4, A5 and A6 ... The pH-induced membrane binding of annexins A6 and A2-S100A10 might have consequences for their functions as membrane ...
more infohttp://www.biochemj.org/content/409/1/65

Annexin A6 - WikipediaAnnexin A6 - Wikipedia

Annexin A6 is a protein that in humans is encoded by the ANXA6 gene. Annexin VI belongs to a family of calcium-dependent ... "Entrez Gene: ANXA6 annexin A6". "Dysmorphology data for Anxa6". Wellcome Trust Sanger Institute. "Haematology data for Anxa6". ... It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Exon 21 of annexin VI is ... The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of ...
more infohttps://en.wikipedia.org/wiki/Annexin_A6

ANXA6 (annexin A6) - KOMP (Knockout Mouse Project)ANXA6 (annexin A6) - KOMP (Knockout Mouse Project)

Vega: OTTMUSG6325 (Anxa6, annexin A6)*CCDS: 24705, 24705.1, 48799*OMIM: ANNEXIN A6; ANXA6*Gene Ontology: Anxa6 *Mouse Phenome ... annexin A6. Synonyms: Annexin VI, Anx6, AnxVI, Cabm, Camb. Gene nomenclature, locus information, and GO, OMIM, and PMID ...
more infohttps://www.komp.org/geneinfo.php?geneid=23392

Recombinant Human Annexin A6, His-tagged ANXA6-1839H - Creative BioMartRecombinant Human Annexin A6, His-tagged ANXA6-1839H - Creative BioMart

Recombinant human Annexin A6 fused to His-tag at N-terminus was expressed inE.coliand purified by using conventional ... ANXA6 annexin A6 [ Homo sapiens ]. Synonyms:. annexin A6; ANX6; CBP68; ANXA6; p68; p70; CPB-II; annexin-6; calelectrin; ... Recombinant Human Annexin A6, His-tagged. Download Datasheet See All ANXA6 Products. Bring this labeled protein directly to ... Recombinant human Annexin A6 fused to His-tag at N-terminus was expressed inE.coliand purified by using conventional ...
more infohttps://www.creativebiomart.net/description_6804_17.htm

Annexin A6 inhibits Ras signaling in breast cancer cells.Annexin A6 inhibits Ras signaling in breast cancer cells.

Recently, we showed that in EGFR overexpressing A431 cells, which lack endogenous Annexin A6 (AnxA6), ectopic expression of ... Recently, we showed that in EGFR overexpressing A431 cells, which lack endogenous Annexin A6 (AnxA6), ectopic expression of ... Annexin A6 inhibits Ras signaling in breast cancer cells.. Abstract. Overexpression of epidermal growth factor receptor (EGFR) ... Recently, we showed that in EGFR overexpressing A431 cells, which lack endogenous Annexin A6 (AnxA6), ectopic expression of ...
more infohttps://www.garvan.org.au/research/publications/10032

Annexin A6 regulates interleukin-2-mediated T-cell proliferation by Rhea Cornely, Abigail H. Pollock et al."Annexin A6 regulates interleukin-2-mediated T-cell proliferation" by Rhea Cornely, Abigail H. Pollock et al.

Annexin A6 (AnxA6) has been implicated in cell signalling by contributing to the organisation of the plasma membrane. Here we ... Annexin A6 (AnxA6) has been implicated in cell signalling by contributing to the organisation of the plasma membrane. Here we ... Annexin A6 regulates interleukin-2-mediated T-cell proliferation. Immunology and Cell Biology, 94 (6), 543-553. ...
more infohttps://ro.uow.edu.au/ihmri/931/

Annexin A6, 1-673aa, Human, 01-2062-6  | ARP American Research Products, Inc.Annexin A6, 1-673aa, Human, 01-2062-6 | ARP American Research Products, Inc.

ANXA6, ANX6, CBP68, 67 kDa calelectrin, Annexin A6, Annexin VI p68, AnnexinA6, AnnexinVI, ANX 6, ANX A6, ANXA 6, Calcium ... Annexin A6, 1-673aa, Human. Background. Annexin A6 belongs to a family of calcium-dependent membrane and phospholipid binding ... Recombinant Annexin A6 protein was expressed in E.coli and purified by using conventional chromatography techniques.. ... Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane- ...
more infohttps://www.arp1.com/suppliers/arp/annexin-a6-1-673aa-human-his-tag-e-coli.html

Depletion of annexin A5, annexin A6 and collagen X causes no gross changes in matrix vesicle mediated mineralization | Murdoch...Depletion of annexin A5, annexin A6 and collagen X causes no gross changes in matrix vesicle mediated mineralization | Murdoch...

Depletion of annexin A5, annexin A6 and collagen X causes no gross changes in matrix vesicle mediated mineralization. Pubmed ID ... Depletion of annexin A5, annexin A6 and collagen X causes no gross changes in matrix vesicle mediated mineralization. Journal ...
more infohttps://www.mcri.edu.au/publications/depletion-annexin-a5-annexin-a6-and-collagen-x-causes-no-gross-changes-matrix-vesicle

Annexin A6 and Late Endosomal Cholesterol Modulate Integrin Recycling and Cell Migration. - Semantic ScholarAnnexin A6 and Late Endosomal Cholesterol Modulate Integrin Recycling and Cell Migration. - Semantic Scholar

Earlier studies implicated annexin A6 (AnxA6) to inhibit secretion and participate in the organization of the extracellular ... Annexins are a family of proteins that bind to phospholipids in a calcium-dependent manner. ... Annexin A6 is a scaffold for PKCα to promote EGFR inactivation. *M Koese, C Rentero, +13 authors T Grewal ... Annexin A6 inhibits Ras signalling in breast cancer cells. *S Vilá de Muga, P Timpson, +11 authors T Grewal ...
more infohttps://www.semanticscholar.org/paper/Annexin-A6-and-Late-Endosomal-Cholesterol-Modulate-Garc%C3%ADa-Melero-Reverter/60e0b347ec010994d45a158c16b80519511f8340

Role of hepatic Annexin A6 in fatty acid-induced lipid droplet formation  - University of Regensburg Publication ServerRole of hepatic Annexin A6 in fatty acid-induced lipid droplet formation - University of Regensburg Publication Server

Annexin A6 (AnxA6) has been implicated in the regulation of endo-/exocytic pathways, cholesterol transport, and the formation ... Annexin A6 (AnxA6) has been implicated in the regulation of endo-/exocytic pathways, cholesterol transport, and the formation ... Role of hepatic Annexin A6 in fatty acid-induced lipid droplet formation ... CHOLESTEROL TRANSPORT; PHOSPHOLIPASE A(2); PROTEINS; MICE; DEGRADATION; TRAFFICKING; AUTOPHAGY; GOLGI; LIVER; VI; Annexin A6; ...
more infohttps://epub.uni-regensburg.de/39170/

JCI -
Recombinant annexin A6 promotes membrane repair and protects against muscle injuryJCI - Recombinant annexin A6 promotes membrane repair and protects against muscle injury

Annexin A6 overexpression reduced membrane injury, consistent with enhanced repair. Treatment with recombinant annexin A6 ... and annexin A6 was previously identified as a genetic modifier of muscle injury and disease. Annexin A6 forms the repair cap ... These data identify annexins as mediators of membrane-associated Ca2+ release during membrane repair and annexin A6 as a ... To elucidate how annexins facilitate repair, we visualized annexin cap formation during injury. We found that annexin cap size ...
more infohttps://huanyu58.com.insight.mobile.jci.org/articles/view/128840/ga

JCI -
Recombinant annexin A6 promotes membrane repair and protects against muscle injuryJCI - Recombinant annexin A6 promotes membrane repair and protects against muscle injury

In contrast, annexin A6 contains 2 annexin cores and thus 8 annexin-repeat domains (22). Annexin A6s duplicated structure ... A6+A6) or wild-type plus mutant (A6+A6E233A) annexin combinations. Mutation of E233 in annexin A6 acted in a dominant-negative ... annexin A1 (NM_010730), annexin A2 (NM_007585), annexin A6 (NM_013472), and annexin A6-encoding sequencing from multiple ... and A6, but was most evident for annexin A2 and A6 (Figure 3A). Over the 240 seconds of imaging, overexpression of annexin A6 ...
more infohttps://yctianyuan.net.insight.mobile.jci.org/articles/view/128840

JCI -
Recombinant annexin A6 promotes membrane repair and protects against muscle injuryJCI - Recombinant annexin A6 promotes membrane repair and protects against muscle injury

In contrast, annexin A6 contains 2 annexin cores and thus 8 annexin-repeat domains (22). Annexin A6s duplicated structure ... A6+A6) or wild-type plus mutant (A6+A6E233A) annexin combinations. Mutation of E233 in annexin A6 acted in a dominant-negative ... annexin A1 (NM_010730), annexin A2 (NM_007585), annexin A6 (NM_013472), and annexin A6-encoding sequencing from multiple ... and A6, but was most evident for annexin A2 and A6 (Figure 3A). Over the 240 seconds of imaging, overexpression of annexin A6 ...
more infohttps://tszhwy.com.insight.mobile.jci.org/articles/view/128840

Calcium- and proton-dependent relocation of annexin A6 in Jurkat T cells stimulated for interleukin-2 secretion - Acta...Calcium- and proton-dependent relocation of annexin A6 in Jurkat T cells stimulated for interleukin-2 secretion - Acta...

Annexin A6 (AnxA6) is a Ca2+-dependent membrane-binding protein involved in vesicular traffic. The likely participation of ... Calcium- and proton-dependent relocation of annexin A6 in Jurkat T cells stimulated for interleukin-2 secretion. ... Grewal T, Enrich C (2006) Molecular mechanisms involved in Ras inactivation: the annexin A6-p120GAP complex. BioEssays 28: 1211 ... GTP-induced membrane binding and ion channel activity of annexin VI: is annexin VI a GTP biosensor? Biophys J 82: 2737-2745. ...
more infohttp://psjd.icm.edu.pl/psjd/element/bwmeta1.element.bwnjournal-article-abpv54p261kz

Expression of ANXA6 in cancer - Summary - The Human Protein AtlasExpression of ANXA6 in cancer - Summary - The Human Protein Atlas

Annexin A6 (HGNC Symbol). Entrez gene summary. Annexin VI belongs to a family of calcium-dependent membrane and phospholipid ... Annexin A6. Protein classi. Protein class the gene product belongs to according to selected gene lists. List of protein classes ... It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been ... The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of ...
more infohttp://www.proteinatlas.org/ENSG00000197043-ANXA6/pathology

PKM2 Antibody (NBP1-48308): Novus BiologicalsPKM2 Antibody (NBP1-48308): Novus Biologicals

Rabbit Polyclonal Anti-PKM2 Antibody cited in 11 publications. Validated: WB, Simple Western, ICC/IF, IHC, IHC-P. Tested Reactivity: Human, Mouse, Rat, and more.
more infohttps://www.novusbio.com/products/pkm2-antibody_nbp1-48308

p70 S6 Kinase/S6K Antibody (1E12.) (H00006198-M01): Novus Biologicalsp70 S6 Kinase/S6K Antibody (1E12.) (H00006198-M01): Novus Biologicals

Mouse Monoclonal Anti-p70 S6 Kinase/S6K Antibody (1E12.). Validated: WB, ELISA, ICC/IF, IHC-P. Tested Reactivity: Human. 100% Guaranteed.
more infohttps://www.novusbio.com/products/p70-s6-kinase-s6k-antibody-1e12_h00006198-m01

Calcium Regulation in the Cardiac Cell (Homo sapiens) - WikiPathwaysCalcium Regulation in the Cardiac Cell (Homo sapiens) - WikiPathways

... annexin A6/, ,Graphics CenterX=392.6666666666667 CenterY=422.0 Width=60.0 Height=20.0 ZOrder=32768 FontSize=10 ...
more infohttps://www.wikipathways.org/index.php?title=Pathway:WP536&action=edit&oldid=67774

BackgroundBackground

Annexin A6. gi,576697441. 0,00147718. 0,00481598. 0,017. 3,3. Post-translational modification, protein turnover, and chaperones ...
more infohttps://stacks.cdc.gov/view/cdc/34694/cdc_34694_DS13.txt

Plasma membrane damage caused by listeriolysin O is not repaired through endocytosis of the membrane pore | Biology OpenPlasma membrane damage caused by listeriolysin O is not repaired through endocytosis of the membrane pore | Biology Open

S4). Many of the LLO-A647-punctae co-localised with specific annexin A6-positive assemblies verifying annexin A6 can be used to ... During the annexin response, very few GRAF1 carriers were detected, but following loss of the annexin A6 signal, an extensive ... Pore formation was detected using annexin A6, which is one of the most Ca2+ sensitive annexins and has been shown to ... annexin A6 served as an internal control for toxin overload in cells. In cells unable to repair the LLO induced damage, annexin ...
more infohttp://bio.biologists.org/content/7/10/bio035287

Papillary Hidradenoma disease: Malacards - Research Articles, Drugs, Genes, Clinical TrialsPapillary Hidradenoma disease: Malacards - Research Articles, Drugs, Genes, Clinical Trials

Annexin A6. Protein Coding. 5.6. DISEASES inferred 15 12. CTNNBL1 Catenin Beta Like 1. Protein Coding. 4.86. DISEASES inferred ... An important gene associated with Papillary Hidradenoma is ANXA11 (Annexin A11), and among its related pathways/superpathways ...
more infohttps://www.malacards.org/card/papillary_hidradenoma

BBA - Molecular Cell Research (v.1864, #6) | www.chemweb.comBBA - Molecular Cell Research (v.1864, #6) | www.chemweb.com

Annexin A6 (AnxA6) belongs to the conserved annexin family - a group of Ca2 +-dependent membrane binding proteins. AnxA6 is the ... Annexin A6 in the liver: From the endocytic compartment to cellular physiology by Carlos Enrich; Carles Rentero; Thomas Grewal ... Keywords: Annexin A6; Autophagosomes; Ca2 +-binding proteins; Hepatocytes; Intracellular trafficking; Liver regeneration; ... Annexin A2 is involved in Ca2 +-dependent plasma membrane repair in primary human endothelial cells by Sophia Nina Koerdt; ...
more infohttps://chemweb.com/articles/01674889/18640006

JCI -
WelcomeJCI - Welcome

Annexin A6 overexpression reduced membrane injury, consistent with enhanced repair. Treatment with recombinant annexin A6 ... and annexin A6 was previously identified as a genetic modifier of muscle injury and disease. Annexin A6 forms the repair cap ... These data identify annexins as mediators of membrane-associated Ca2+ release during membrane repair and annexin A6 as a ... To elucidate how annexins facilitate repair, we visualized annexin cap formation during injury. We found that annexin cap size ...
more infohttps://www.jci.org/tags/30?content=articles&

Frontiers | Myocardial Contractile Dysfunction Is Present without Histopathology in a Mouse Model of Limb-Girdle Muscular...Frontiers | Myocardial Contractile Dysfunction Is Present without Histopathology in a Mouse Model of Limb-Girdle Muscular...

2014). Annexin A6 modifies muscular dystrophy by mediating sarcolemmal repair. Proc. Natl. Acad. Sci. U.S.A. 111, 6004-6009. ... Annexin-6 has also recently been demonstrated to be a strong genetic modifier of muscle membrane repair in sarcoglyan knockout ...
more infohttps://www.frontiersin.org/articles/10.3389/fphys.2016.00539/full

Lipid Metabolism sub-cluster 33Lipid Metabolism sub-cluster 33

Vitamin A (all-trans retinol) and all-trans retinoid acid (ATRA) interacted with human annexin A6 (AnxA6) as evidenced by AnxA6 ...
more infohttp://www.biomedsearch.com/cluster/14/Lipid-Metabolism/sub-33-p6.html
  • Annexin A6 inhibits Ras signaling in breast cancer cells. (garvan.org.au)
  • We also found that annexin overexpression promoted external blebs enriched in Ca2+ and correlated with a reduction of intracellular Ca2+ at the injury site. (jci.org)
  • Annexin A6 (AnxA6) has been implicated in cell signalling by contributing to the organisation of the plasma membrane. (edu.au)
  • Annexin A6 and Late Endosomal Cholesterol Modulate Integrin Recycling and Cell Migration. (semanticscholar.org)