Authorship: The profession of writing. Also the identity of the writer as the creator of a literary production.Knowledge Bases: Collections of facts, assumptions, beliefs, and heuristics that are used in combination with databases to achieve desired results, such as a diagnosis, an interpretation, or a solution to a problem (From McGraw Hill Dictionary of Scientific and Technical Terms, 6th ed).Annexin A2: A member of the annexin family that is a substrate for a tyrosine kinase, ONCOGENE PROTEIN PP60(V-SRC). Annexin A2 occurs as a 36-KDa monomer and in a 90-KDa complex containing two subunits of annexin A2 and two subunits of S100 FAMILY PROTEIN P11. The monomeric form of annexin A2 was formerly referred to as calpactin I heavy chain.Annexin A1: Protein of the annexin family exhibiting lipid interaction and steroid-inducibility.Annexin A5: A protein of the annexin family isolated from human PLACENTA and other tissues. It inhibits cytosolic PHOSPHOLIPASE A2, and displays anticoagulant activity.Annexin A6: Protein of the annexin family with a probable role in exocytotic and endocytotic membrane events.Editorial Policies: The guidelines and policy statements set forth by the editor(s) or editorial board of a publication.Scientific Misconduct: Intentional falsification of scientific data by presentation of fraudulent or incomplete or uncorroborated findings as scientific fact.Annexin A4: Protein of the annexin family originally isolated from the electric organ of the electric ray Torpedo marmorata. It has been found in a wide range of mammalian tissue where it is localized to the apical membrane of polarized EPITHELIAL CELLS.Publishing: "The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Antibody Specificity: The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.Antibodies, Viral: Immunoglobulins produced in response to VIRAL ANTIGENS.beta 2-Glycoprotein I: A 44-kDa highly glycosylated plasma protein that binds phospholipids including CARDIOLIPIN; APOLIPOPROTEIN E RECEPTOR; membrane phospholipids, and other anionic phospholipid-containing moieties. It plays a role in coagulation and apoptotic processes. Formerly known as apolipoprotein H, it is an autoantigen in patients with ANTIPHOSPHOLIPID ANTIBODIES.Antibodies, Bacterial: Immunoglobulins produced in a response to BACTERIAL ANTIGENS.Phospholipids: Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.C-Reactive Protein: A plasma protein that circulates in increased amounts during inflammation and after tissue damage.Macrophages: The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)Phosphatidylcholines: Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a choline moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and choline and 2 moles of fatty acids.Lipoproteins, LDL: A class of lipoproteins of small size (18-25 nm) and light (1.019-1.063 g/ml) particles with a core composed mainly of CHOLESTEROL ESTERS and smaller amounts of TRIGLYCERIDES. The surface monolayer consists mostly of PHOSPHOLIPIDS, a single copy of APOLIPOPROTEIN B-100, and free cholesterol molecules. The main LDL function is to transport cholesterol and cholesterol esters to extrahepatic tissues.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Search Engine: Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.Databases, Genetic: Databases devoted to knowledge about specific genes and gene products.Genome, Human: The complete genetic complement contained in the DNA of a set of CHROMOSOMES in a HUMAN. The length of the human genome is about 3 billion base pairs.Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.

Binding of annexin V to plasma membranes of human spermatozoa: a rapid assay for detection of membrane changes after cryostorage. (1/1343)

When the cell membrane is disturbed, phospholipid phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane. This is one of the earliest signs of apoptosis and can be monitored by the calcium-dependent binding of annexin V. Therefore, annexin V-binding, in conjunction with flow cytometry, was used to evaluate the integrity of the sperm plasma membrane after different cryostorage protocols: i.e. 10% (v/v) glycerol; sperm maintenance medium (MM); freezing medium TEST yolk buffer (TYB); or cryostorage without protection (cryoshock). Using a combination of two fluorescent dyes, annexin V and propidium iodide (PI), led to three groups of spermatozoa being identified: (i) viable spermatozoa (annexin V-negative and PI-negative); (ii) dead spermatozoa (annexin V-positive and PI-positive); and (iii) cells with impaired but integer plasma membrane (annexin V-positive and PI-negative). The percentage of vital annexin V-negative spermatozoa increased significantly (P < 0.05) from spermatozoa treated by cryoshock (15.0+/-1.2%) to spermatozoa cryopreserved by TYB (26.6+/-2.2%) via cryopreservation by 10% (v/v) glycerol (19.9+/-1.6%) and by MM (22.2 1.8%) and was associated with the percentage of motile spermatozoa (17.6+/-3.4% by glycerol; 19.6+/-3.7% by MM and 22.6+/-3.9% by TYB; P = 0.0001). Of the spermatozoa, 12-22% were annexin V-positive even though they did not bind to PI, indicating viability before as well as after cryostorage. The percentage of vital annexin V-positive spermatozoa was significantly correlated with different sperm motility parameters (velocity straight linear, r = 0.601, P = 0.018; percentage of linearly motile spermatozoa: r = 0.549, P = 0.034). We, therefore, concluded that annexin V-binding is more sensitive in detecting a deterioration of membrane functions than PI staining, and that a considerable percentage of spermatozoa might have dysfunctional plasma membranes besides dead or moribund cells. Of the cryopreservation protocols tested, TYB yielded the most viable spermatozoa. Therefore, we advocate the use of the annexin V-binding assay for the evaluation of the quality and integrity of spermatozoa.  (+info)

Overexpression of RelA causes G1 arrest and apoptosis in a pro-B cell line. (2/1343)

NF-kappaB/Rel family proteins form a network of post-translationally regulated transcription factors that respond to a variety of extracellular stimuli and mediate distinct cellular responses. These responses include cytokine gene expression, regulated cell cycle activation, and both the protection from and induction of the cell death program. To examine the function of individual Rel family proteins in B cell development and resolve their role in the signaling of apoptosis, we used a tetracycline-regulated gene expression system to overexpress either c-Rel or RelA in the transformed pro-B cell line 220-8. Elevated levels of RelA, but not c-Rel, induced a G1 cell cycle arrest followed by apoptosis. Both the DNA binding and transactivation domains of RelA were required for this effect. When RelA was overexpressed in the immature B cell line WEHI 231 or the mature B cell line M12, neither cell cycle arrest nor apoptosis was evident. The differential effects of elevated RelA levels in these cell lines suggests that susceptibility to NF-kappaB-induced apoptosis may reflect a relevant selection event during B cell development.  (+info)

Sodium salicylate activates caspases and induces apoptosis of myeloid leukemia cell lines. (3/1343)

Nonsteroidal antiinflammatory agents (NSAIA) have been shown to exert potent chemopreventive activity against colon, lung, and breast cancers. In this study, we show that at pharmacological concentrations (1 to 3 mmol/L) sodium salicylate (Na-Sal) can potently induce programmed cell death in several human myeloid leukemia cell lines, including TF-1, U937, CMK-1, HL-60, and Mo7e. TF-1 cells undergo rapid apoptosis on treatment with Na-Sal, as indicated by increased annexin V binding capacity, cpp-32 (caspase-3) activation, and cleavage of poly (ADP-ribose) polymerase (PARP) and gelsolin. In addition, the expression of MCL-1, an antiapoptotic member of the BCL-2 family, is downregulated during Na-Sal-induced cell death, whereas the expression of BCL-2, BAX, and BCL-XL is unchanged. Z-VAD, a potent caspase inhibitor, prevents the cleavage of PARP and gelsolin and rescues cells from Na-Sal-induced apoptosis. In addition, we show that Na-Sal accelerates growth factor withdrawal-induced apoptosis and synergizes with daunorubicin to induce apoptosis in TF-1 cells. Thus, our data provide a potential mechanism for the chemopreventive activity of NSAIA and suggest that salicylates may have therapeutic potential for the treatment of human leukemia.  (+info)

Temporal and spatial aspects of fragmentation in early human embryos: possible effects on developmental competence and association with the differential elimination of regulatory proteins from polarized domains. (4/1343)

This study examined the relationship between blastomere fragmentation in cultured human embryos obtained by in-vitro fertilization and the effect of fragmentation on the distribution of the following eight regulatory proteins found to be: (i) localized in the mature oocyte in subplasmalemmal, polarized domains; and (ii) unequally inherited by the blastomeres during cleavage: leptin, signal transducer and activator of transcription 3 (STAT3), Bax, Bcl-x, transforming growth factor beta 2 (TGF beta 2), vascular endothelial growth factor (VEGF), c-kit and epidermal growth factor R (EGF-R). Four basic patterns of fragmentation were observed. The severity of the impact of each type of fragmentation on the affected blastomere(s) and the developmental competence of the embryo appeared to be a function of the unique temporal and spatial features associated with the particular fragmentation pattern(s) involved in each instance. The findings demonstrate that certain patterns of fragmentation can result in the partial or near total loss of the eight regulatory proteins from specific blastomeres and that the developmental potential of the affected embryo can be particularly compromised if it occurs during the 1- or 2-cell stages. In contrast, fragmentation from portions of a fertilized egg or a blastomere(s) in a 2-cell embryo that do not contain the protein domains, or the complete loss by fragmentation of a regulatory protein domain-containing blastomere after the 4-cell stage does not necessarily preclude continued development to the blastocyst, although the normality and developmental potential of the embryo may be compromised. The possible association between fragmentation and apoptosis was examined by annexin V staining of plasma membrane phosphatidylserine and TUNEL analysis of blastomere DNA. No direct correlation between fragmentation and apoptosis was found following the analyses of fragmented embryos with these two markers. However, while we suggest that changes in cell physiology unrelated to apoptosis are the more likely causes of fragmentation, we cannot exclude the possibility that fragmentation itself may be an initiator of apoptosis if critical ratios or levels of developmentally important proteins are altered by partial or complete elimination of their polarized domains. The findings are discussed with respect to the possible developmental significance of regulatory protein polarization in human oocytes and preimplantation stage embryos.  (+info)

Interaction of heparin with annexin V. (5/1343)

The energetics and kinetics of the interaction of heparin with the Ca2+ and phospholipid binding protein annexin V, was examined and the minimum oligosaccharide sequence within heparin that binds annexin V was identified. Affinity chromatography studies confirmed the Ca2+ dependence of this binding interaction. Analysis of the data obtained from surface plasmon resonance afforded a Kd of approximately 21 nM for the interaction of annexin V with end-chain immobilized heparin and a Kd of approximately 49 nM for the interaction with end-chain immobilized heparan sulfate. Isothermal titration calorimetry showed the minimum annexin V binding oligosaccharide sequence within heparin corresponds to an octasaccharide sequence. The Kd of a heparin octasaccharide binding to annexin V was approximately 1 microM with a binding stoichiometry of 1:1.  (+info)

Oxidant-induced apoptosis in cultured human retinal pigment epithelial cells. (6/1343)

PURPOSE: To determine the mechanism of oxidant-induced cell death in cultured human retinal pigment epithelium (hRPE). METHODS: Cultured hRPE cells were treated with different concentrations of a chemical oxidant, t-butylhydroperoxide (tBH), for different periods of time. Apoptosis was determined with terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) and flow cytometry. Mitochondrial membrane potential (mtdelta psi) was measured by rhodamine 123 staining and subsequent flow cytometry. Release of mitochondrial cytochrome c (cyt c) and cleavage of procaspase 3 and caspase substrates were determined by western blot analysis. RESULTS: t-Butylhydroperoxide caused time- and dose-dependent activation of apoptosis in hRPE, indicated by characteristic morphologic changes; TUNEL-positive labeling; phosphatidylserine (PS) exposure; and procaspase 3, poly(ADP-ribose)polymerase, lamin, and tubulin cleavage. An early decrease of mtdelta psi was observed before caspase activation, together with the release of mitochondrial cyt c. CONCLUSIONS: Results indicate that tBH can induce apoptosis in hRPE, probably by triggering the mitochondrial permeability transition, which results in swelling and release of mitochondrial intermembrane proteins.  (+info)

Functional and structural properties of urinary tissue factor. (7/1343)

BACKGROUND: We have previously explored the clinical significance of urinary tissue factor (uTF) in patients with glomerulonephritis (GN) and malignancy. However, the functional and structural properties and putative cell of origin of uTF are poorly documented. In these studies we investigate these aspects of uTF. METHODS: Functional and structural properties of uTF were investigated using a one stage kinetic chromogenic assay, an enzyme-linked immunoabsorbent assay (ELISA) and transmission electron microscopy (TEM) on urine samples collected from healthy controls (n=69). The distribution of uTF and anionic phospholipid in the kidney were studied in sections from normal areas of nephrectomy specimens, using an immunoperoxidase technique. These were stained for tissue factor (TF) antigen and recombinant Annexin V. RESULTS: We found uTF to be present on subcellular particles as visualized by TEM. These particles contained anionic phospholipid as evidenced by binding to Annexin V fluorescein isothiocyanate (FITC). Although TF is present in urine in a functional and antigenic form no association was observed between the two. Using immunoperoxidase-based techniques, the cytoplasm of both distal and proximal tubules (but not glomerular cells) was positive for TF antigen and Annexin V. CONCLUSION: uTF is found on subcellular particles which provide lipid for its functional activity. Both uTF and its associated vesicles are found in the renal tubular cells.  (+info)

Localization and quantitation of cardiac annexins II, V, and VI in hypertensive guinea pigs. (8/1343)

Annexins are characterized by Ca2+-dependent binding to phospholipids. Annexin II mainly participates in cell-cell adhesion and signal transduction, whereas annexins V and VI also seem to regulate intracellular calcium cycling. Their abundance and localization were determined in left ventricle (LV) and right ventricle (RV) from hypertensive guinea pigs, during the transition from compensatory hypertrophy to heart failure. Immunoblot analysis of annexins II, V, and VI revealed an increased accumulation (2.6-, 1.45-, and 2.3-fold, respectively) in LV from hypertensive guinea pigs and no modification in RV. Immunofluorescent labeling of annexins II, V, and VI; of Na+-K+-ATPase; and of sarcomeric alpha-actinin showed that in control LV and RV, 1) annexin II is present in nonmuscle cells; 2) annexins V and VI are mainly observed in the sarcolemma and intercalated disks of myocytes; 3) annexins II, V, and VI strongly label endothelial cells and adventitia of coronary arteries; and 4) annexin VI is present in the media. At the onset of heart failure, the most striking changes are the increased protein accumulation in LV and the very strong labeling of annexins II, V, and VI in interstitial tissue, suggesting a role in fibrosis development and cardiac remodeling.  (+info)

*Annexin A5

... (or annexin V) is a cellular protein in the annexin group. In flow cytometry, annexin V is commonly used to detect ... Annexin A5 showed upregulation in papillary thyroid carcinoma. Annexin A5 is used as a non-quantitative probe to detect cells ... Annexin A5 forms a shield around negatively charged phospholipid molecules. The formation of an annexin A5 shield blocks the ... The annexin A5 affinity assay typically uses a conjugate of annexin V and a fluorescent or enzymatic label, biotin or other ...

*Annexin A3

... is a protein that in humans is encoded by the ANXA3 gene. It is abnormally expressed in fetuses of both IVF and ICSI ... "Entrez Gene: ANXA3 annexin A3". Zhang Y, Zhang YL, Feng C, et al. (September 2008). "Comparative proteomic analysis of human ... 2005). "Annexin A3 is a potential angiogenic mediator". Biochem. Biophys. Res. Commun. 337 (4): 1283-7. doi:10.1016/j.bbrc. ... "The high-resolution crystal structure of human annexin III shows subtle differences with annexin V". Biochemistry. 35 (6): 1740 ...

*Annexin A4

... is a protein that in humans is encoded by the ANXA4 gene. Annexin IV (ANX4) belongs to the annexin family of calcium ... "Entrez Gene: ANXA4 annexin A4". Human ANXA4 genome location and ANXA4 gene details page in the UCSC Genome Browser. Römisch J, ... Dreier R, Schmid KW, Gerke V, Riehemann K (Aug 1998). "Differential expression of annexins I, II and IV in human tissues: an ... Han EK, Tahir SK, Cherian SP, Collins N, Ng SC (Jul 2000). "Modulation of paclitaxel resistance by annexin IV in human cancer ...

*Annexin A5 affinity assay

This clustering of annexin A5 on the membrane greatly increases the intensity of annexin A5 when labeled with a fluorescent or ... Labeling of annexin A5 with fluorescent or radioactive molecules makes it possible to detect binding of labeled annexin A5 to ... Internalization amplifies additionally the intensity of the annexin A5 stained cell. Annexin A5 has been used to successively ... annexin A5 assembles into a trimeric cluster. This trimer consists of three annexin A5 molecules that are bound to each other ...

*Antiphospholipid syndrome

Annexin A5 forms a shield around negatively charged phospholipid molecules, thus reducing their availability for coagulation. ... Thus, anti-annexin A5 antibodies increase phospholipid-dependent coagulation steps. The Lupus anticoagulant antibodies are ...

*Integrin, beta 5

... has been shown to interact with PTK2, Annexin A5 and PAK4. Elevated levels of ITGB5 have been found in ... "Alpha v beta 5 integrin-dependent programmed cell death triggered by a peptide mimic of annexin V". Mol. Cell. United States. ...

*Kinase insert domain receptor

Annexin A5 and SHC1. Cluster of differentiation VEGF receptors Holmes K, Roberts OL, Thomas AM, Cross MJ (October 2007). " ...

*Annexin

However some annexins (Annexin A1, Annexin A2, and Annexin A5) have also been found outside the cellular environment, for ... Annexin, type I InterPro: IPR002388 Annexin, type II InterPro: IPR002389 Annexin, type III InterPro: IPR002390 Annexin, type IV ... In addition to annexin A-II, annexin A-XI has also been shown to organize cell membrane properties. Annexin A-XI is believed to ... The 310 amino acid annexin core has four annexin repeats, each composed of 5 alpha-helices. The exception is annexin A-VI that ...

*Annexin Pharmaceuticals

The concept for the therapy is based on the anti-inflammatory properties of Annexin A5, a body own protective protein that acts ... Annexin Pharmaceuticals is a Swedish privately held biotech company developing new therapeutic approaches for inflammatory ... http://www.solidinfo.se/foretag/annexin-pharmaceuticals-ab/personer. ... "Annexin: Attenuating vascular inflammation" (PDF). BioCentury: A18. Retrieved 1 February 2015. ...

*Flow cytometry

Annexin A5 affinity assay, a test for cells undergoing apoptosis, often uses flow cytometry Cell cycle analysis Coulter counter ...

*List of MeSH codes (D08)

... annexin A3 MeSH D08.811.277.352.640.125 --- 3',5'-cyclic-GMP phosphodiesterase MeSH D08.811.277.352.640.150 --- 3',5'-cyclic- ... cytochromes a3 MeSH D08.244.187.249 --- cytochromes b MeSH D08.244.187.500 --- cytochromes b5 MeSH D08.244.187.600 --- ...

*List of MeSH codes (D12.776.157)

Annexin A3 MeSH D12.776.157.125.050.080 -- annexin a4 MeSH D12.776.157.125.050.100 -- annexin a5 MeSH D12.776.157.125.050.110 ... annexin a6 MeSH D12.776.157.125.050.120 -- annexin a7 MeSH D12.776.157.125.412.249 -- calmodulin MeSH D12.776.157.125.412.311 ... annexin a1 MeSH D12.776.157.125.050.060 -- annexin a2 MeSH D12.776.157.125.050.070 -- ...

*A3

... regulatory sequence, a sequence for the insulin gene Adenosine A3 receptor, a human gene Annexin A3, a human gene ATC code ... a band known as A3 in the U.S. to avoid confusion with the country group Alabama A-3, a Yamaha musical instrument product A3 ... a club football tournament also known as the East Asian Champions Cup Arrows A3, a 1980 racing car A3, a climbing Grade A-3 ... a British Isles plants community Gibberellin A3, a plant hormone HLA-A3, a Human MHC Serotype HLA-A Subfamily A3, a rhodopsin- ...

*A5

... and variants may refer to: A5 regulatory sequence in biochemistry Annexin A5, a human cellular protein ATC code A05 Bile and ... see section Military Chery A5, a 2006 Chinese car Audi A5, a 2007 luxury coupé Arrows A5, a 1982 British racing car A5 road, in ... the Apple mobile microprocessor ARM Cortex-A5, ARM applications processor A5/1, A5/2 and A5/3, ciphers used in cellular phones ... A-5, an export version of Nanchang Q-5, a Chinese-built jet fighter bomber Curtiss Falcon or A-5 Falcon, an attack aircraft ...

*S100A10

Annexin II, which is attracted to negatively charged phospholipids, binds to p11 at the Ca2+ binding site. In addition, Annexin ... "Induction of endogenous genes following infection of human endothelial cells with an E1(-) E4(+) adenovirus gene transfer ... Kwon M, MacLeod TJ, Zhang Y, Waisman DM (Jan 2005). "S100A10, annexin A2, and annexin a2 heterotetramer as candidate ... The p11 and annexin II complex is regulated by the phosphorylation of SerII on the annexin II molecule by protein kinase C (PKC ...

*Formyl peptide receptor 3

Takano T, Fiore S, Maddox JF, Brady HR, Petasis NA, Serhan CN (May 1997). "Aspirin-triggered 15-epi-lipoxin A4 (LXA4) and LXA4 ... Ernst S, Lange C, Wilbers A, Goebeler V, Gerke V, Rescher U (Jun 2004). "An annexin 1 N-terminal peptide activates leukocytes ... "The synthetic peptide Trp-Lys-Tyr-Met-Val-Met-NH2 specifically activates neutrophils through FPRL1/lipoxin A4 receptors and is ... is not affected by lipoxin A4". Scandinavian Journal of Immunology. 56 (5): 470-6. doi:10.1046/j.1365-3083.2002.01149.x. PMID ...

*Proliferating cell nuclear antigen

Annexin A2, CAF-1, CDC25C, CHTF18, Cyclin D1, Cyclin O, Cyclin-dependent kinase 4, Cyclin-dependent kinase inhibitor 1C, DNMT1 ... E3 ubiquitin ligases • E2 SUMO-conjugating enzyme • Helicases, ATPases • Mismatch repair enzymes • Base excision repair enzymes ...

*Human papillomavirus infection

The HPV genome is composed of six early (E1, E2, E4, E5, E6, and E7) open-reading frames (ORF), two late (L1 and L2) ORFs, and ... and annexin A2 leading to entry of the virions into basal epithelial cells through clathrin-mediated endocytosis and/or ... "The S100A10 subunit of the annexin A2 heterotetramer facilitates L2-mediated human papillomavirus infection". PLoS One. 7 (8): ...

*Formyl peptide receptor 2

... lipoxin A4 (LXA4). Because of its interaction with lipoxin A4, FPR2 is also commonly named the ALX/FPR2 or just ALX receptor. ... Ac2-26 and Ac9-25 of Annexin A1 (ANXA1 or lipocortin 1), which at high concentrations fully stimulate neutrophil functions but ... "Lipoxin A4 stable analogs are potent mimetics that stimulate human monocytes and THP-1 cells via a G-protein-linked lipoxin A4 ... lipoxin A4 (LXA4), and thereafter for a related arachidonic acid metabolite, the Epi-lipoxin, aspirin-triggered lipoxin A4 (i.e ...

*Housekeeping gene

018229 ANXA6 Annexin 6 ANXA7 Annexin 7 AP1B1 Coated vessicles CLTA Clathrin A (vessicles) CLTB Clathrin B (vessicles) CLTC MTX2 ... 021178 E3 ubiquitin-protein ligase. Modulates cyclin B levels and participates in the regulation of cell cycle progression ... 003334 Homo sapiens ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature UBA2 NM_005499 UBA3 NM_003968 UBA5 NM_024818 ...

*Specialized pro-resolving mediators

These products are now termed lipoxin A4 and B4, respectively. While initially found to have in vitro activity suggesting that ... annexin A1 (an inhibitor of formation of pro-inflammatory metabolites of polyunsaturated fatty acids), and the gaseous ...

*Ubiquitin C

He KL, Deora AB, Xiong H, Ling Q, Weksler BB, Niesvizky R, Hajjar KA (July 2008). "Endothelial cell annexin A2 regulates ... "Cancer related mutations in NRF2 impair its recognition by Keap1-Cul3 E3 ligase and promote malignancy". Proceedings of the ... "cIAP1 and cIAP2 facilitate cancer cell survival by functioning as E3 ligases that promote RIP1 ubiquitination". Molecular Cell ... "Unanchored K48-linked polyubiquitin synthesized by the E3-ubiquitin ligase TRIM6 stimulates the interferon-IKKε kinase-mediated ...

*Formyl peptide receptor 1

Takano T, Fiore S, Maddox JF, Brady HR, Petasis NA, Serhan CN (May 1997). "Aspirin-triggered 15-epi-lipoxin A4 (LXA4) and LXA4 ... Annexin A1 (also termed ANXA1 and lipocortin 1) and its N-terminal peptides (Ac2-26 and Ac9-25). At low concentrations, these ... FMLP and lipoxin A4. Isolated mouse Olfactory bulb neurons also respond to a range of other fpr agonists. These results suggest ... and functional characterization of a murine lipoxin A4 receptor homologue gene". Journal of Immunology. 169 (6): 3363-9. doi: ...

*Estrogen

The three major naturally occurring forms of estrogen in women are estrone (E1), estradiol (E2), and estriol (E3). Another type ... Nadkarni S, Cooper D, Brancaleone V, Bena S, Perretti M (November 2011). "Activation of the annexin A1 pathway underlies the ... The three major naturally occurring estrogens in women are estrone (E1), estradiol (E2), and estriol (E3). Estradiol is the ... A4), androstenediol (A5), 3α-androstanediol, and 3β-androstanediol. Some estrogen metabolites, such as the catechol estrogens 2 ...
Conclusion These results indicated that these antibodies may not be associated with the pathogenesis of thrombotic events of the patients with Behçets syndrome.. IgG anti-annexin V antibodies were detected in 5 of 27 healthy subjects (18%).. Compare to previous study results this may indicate the possibility of high prevalence of IgG anti-annexin V antibodies in the healthy Turkish population. ...
IgG was the most frequently detected isotype in all four analyzed aPL subtypes. According to diagnostic criteria, aCL were more frequently found in patients with pAPS and sAPS than in those with SLE. Similarly, aPT were more often elevated in patients with pAPS (7/10) and sAPS (6/19) than in SLE (16/63; P , 0.05). The prevalence of anti-β2-GPI and aANXV antibodies did not differ between patient groups. Regardless of diagnosis, aPT were always detected in combination with aCL and/or anti-β2-GPI. aPT and aANXV were regularly present in the sera positive also for aCL and/or anti-β2-GPI. aANXV was detected as a single antibody in only one patient. In the pAPS group concurrent multiple aPL varieties (three or four) were significantly more frequently present than one or two types (8/10 versus 2/10; P , 0.05). Statistically significant associations of particular aPL with clinical symptoms were as follows: elevated IgG anti-β2-GPI with arterial thromboses, thrombocytopenia and foetal loss; IgG aPT ...
18F-FDG, a marker of the enhanced metabolism characteristic of activated inflammatory cells, and 99mTc-annexin A5, a marker of apoptosis, are both widely believed to be useful for the imaging of unstable atheroma (rupture-prone vulnerable plaques [VP]). Serum cholesterol functions as a proinflammatory factor, driving the formation of VP, and affects the immune responses of aortic tissues systemically. It is therefore reasonable to postulate that prolonged cholesterol loading may alter the aortic uptake of these tracers. Here, we evaluated the aortic uptake of 18F-FDG and 99mTc-annexin A5 in apolipoprotein E-deficient (apoE2/2) and wild-type mice placed on high-fat diets. Methods: Male apoE2/2 and wild-type (C57BL/6J) mice were maintained on high-fat diets after the age of 5 wk. Wild-type mice fed regular chow were used as controls. At the ages of 10, 18, and 25 wk (5-15 mice per group at each time point), mice were injected with 18F-FDG or 99mTc-annexin A5 after 12 h of fasting. At 1 h after ...
ELISA method for the measurement of IgM isotype auto antibodies targeted to annexin V. Assay designed with highly purified human recombinant annexin V, obtained with non-denaturing methods, for auto antibody immunocapture. These are then revealed with a goat antibody specific for IgM, micro fragments, and labeled with peroxidase.
1HVE: Structural and electrophysiological analysis of annexin V mutants. Mutagenesis of human annexin V, an in vitro voltage-gated calcium channel, provides information about the structural features of the ion pathway, the voltage sensor and the ion selectivity filter
A human ex vivo thrombosis model was used to investigate whether recombinant annexin V (rANV) can prevent thrombus formation under venous and arterial blood flow conditions. In this model, blood from an antecubital vein of healthy donors was allowed to flow directly over the extracellular matrix of tumor necrosis factor-stimulated endothelial cells (TNF-ECMs). TNF-ECMs were preincubated with rANV (2.9 mumol/L) for 30 minutes. With this rANV concentration all binding sites present on TNF-ECMs (1.6 +/- 0.5 x 10(12)/cm2) are occupied, and a maximal inhibition was observed in a tissue factor-dependent clotting assay. Fibrin deposition and platelet and leukocyte adhesion were measured on the rANV-treated and nontreated TNF-ECMs. Nontreated TNF-ECMs were used as controls. rANV inhibited fibrin deposition by 81% at a wall shear rate of 100 s-1. A nonsignificant inhibition was also observed at 650 s-1. Platelet-matrix adhesion, which is more prominent at higher shear rates, was significantly decreased ...
Modified annexin proteins, including a homodimer of human annexin V, are provided. Methods for their use, such as to prevent thrombosis without increasing hemorrhage, enhancing the survivability of platelets during storage or transfusion and to attenuate ischemia-reperfusion injury (IPI), are also provided. The modified annexins bind phosphatidylserine (PS) on cell surfaces, thereby preventing the assembly of the prothrombinase complex. The modified annexin decreases the binding of leukocytes and platelets during post-ischemic reperfusion, thereby restoring microvascular blood flow and decreasing organ damage. In addition, the modified annexin prevents lipid loss from platelets during storage.
1HVD: Structural and electrophysiological analysis of annexin V mutants. Mutagenesis of human annexin V, an in vitro voltage-gated calcium channel, provides information about the structural features of the ion pathway, the voltage sensor and the ion selectivity filter
Annexin V is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Annexin V is a monomer, binds ATRX and EIF5B, and interacts with hepatitis B virus (HBV ...
|p|Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry
For those of you who were following the Caspase-3 thread and those of you who contacted me directly, heres the information regarding Caspase-3 and Annexin V that I received from Pharmingen. Any furthur questions, please contact Padma at Pharmingen. Padma was most helpful. Lora , -----Original Message----- , From: pkodukula at pharmingen.com [SMTP:pkodukula at pharmingen.com] , Sent: Monday, October 04, 1999 10:14 AM , To: Barsky, Lora , Subject: RE: caspase-3 , , , , Dear Lora, , , Thank you for your interest in PharMingens products. This is in reply to , your , question about the caspase 3 and the annexin V staining. We are releasing , a kit , for the BrDU and cytokine staining. But the annexin V is also known to , work , with the caspase 3. This is not something that we routinely quality , control in , our company but we have had input from customers who have used this , method. The , annexin V binding to phosphatidyl serine is dependent on the CA++ ion , concentration and so a Ca++ ...
Annexin V-FITC Apoptosis assay results: Flow cytometry profiles represent Annexin-V-FITC staining in X-axis and PI in Y-axis: (a) control, i.e., neither PSi nor
BioAssay record AID 618683 submitted by ChEMBL: Induction of apoptosis in human HepG2 cells assessed as non-viable cells at 6 uL after 18 hrs by annexinV propidium iodide staining based flow cytometric analysis (Rvb = 14.32%).
Rabbit polyclonal Secretogranin V antibody validated for WB, IP, IHC, ICC/IF and tested in Human and Rat. Referenced in 2 publications and 2 independent…
Gentaur molecular products has all kinds of products like :search , Trevigen \ Annexin V FITC Kit \ 4830-01-K for more molecular products just contact us
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I do not know what your doctor is trying to communicate to you. When someone is infected with HIV they develop antibodies to the virus. That is what is meant by being HIV+. It generally takes a...
The surface of healthy cells is composed of lipids that are asymmetrically distributed on the inner and outer leaflet of the plasma membrane. One of these lipids, phosphatidylserine (PS), is normally restricted to the inner leaflet of the plasma membrane and is, therefore, only exposed to the cell cytoplasm. However, during apoptosis lipid asymmetry is lost and PS becomes exposed on the outer leaflet of the plasma membrane. Annexin V, a 36-kDa calcium-binding protein, binds to PS; therefore, fluorescently labeled Annexin V can be used to detect PS that is exposed on the outside of apoptotic cells. Annexin V can also stain necrotic cells because these cells have ruptured membranes that permit Annexin V to access the entire plasma membrane. However, apoptotic cells can be distinguished from necrotic cells by co-staining with propidium iodide (PI) because PI enters necrotic cells but is excluded from apoptotic cells. This protocol describes Annexin V binding and PI uptake followed by flow cytometry ...
The nitrosourea alkylating agent, carmustine, is used as chemotherapeutic drug in several malignancies. The substance triggers tumor cell apoptosis. Side effects of carmustine include myelotoxicity with anemia. At least in theory, anemia could partly be due to stimulation of eryptosis, the suicidal death of erythrocytes, characterized by cell shrinkage and breakdown of phosphatidylserine asymmetry of the cell membrane with phosphatidylserine exposure at the erythrocyte surface. Stimulators of eryptosis include increase of cytosolic Ca2+ activity ([Ca2+]i). The present study tested whether carmustine triggers eryptosis. To this end [Ca2+]i was estimated from Fluo3 fluorescence, cell volume from forward scatter, phosphatidylserine exposure from annexin V binding, and hemolysis from hemoglobin release. As a result a 48 h exposure to carmustine (≥25 µM) significantly increased [Ca2+]i, decreased forward scatter and increased annexin V binding. The effect on annexin V binding was significantly blunted in
BioAssay record AID 541028 submitted by ChEMBL: Induction of apoptosis in bovine BL3 cells assessed as early apoptotic cells after 24 hrs by annexin V/propidium iodide staining-based FACS analysis.
During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca2+-dependent affinity for PS and therefore is used as a probe for detecting apoptosis., Annexin V-FITC Apoptosis Detection Reagent, GTX14082, Applications: FACS; Flow cytometry/FACS; CrossReactivity:
Annexin II antibody (annexin A2) for ICC/IF, IHC-P, IP, RipA, WB. Anti-Annexin II pAb (GTX32446) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
Order monoclonal and polyclonal Annexin A13 antibodies for many applications. Selected quality suppliers for anti-Annexin A13 antibodies.
Group A:. Cycle 1 (3 weeks, combination therapy):. Carboplatin (AUC=5) administered I.V on week 1, day 1. 1000 mg/m ²/d 5-FU administered as a 96-hour C.I. starting on week 1, day 1.. 400 mg/m ² cetuximab administered I.V on week 2, day 1. 250 mg/m ² cetuximab administered I.V on week 3, day 1.. Cycle 2 (3 weeks, combination therapy):. Carboplatin (AUC=5) administered I.V on week 1, day 1. 1000 mg/m ²/d 5-FU administered as a 96-hour C.I. starting on week 1, day 1. 250 mg/m ² cetuximab administered I.V on weeks 1- 3, day 1.. After 7 cycles, participants may then receive cetuximab monotherapy until progression of disease, unacceptable toxicity, or another withdrawal criterion is met.. Due to protocol amendment in September 2011, any newly enrolled participants were placed into cetuximab and carboplatin (C) arm only. After protocol amendment February 2014, any newly enrolled participants will be placed into Group D arm only. ...
2015. Ghelani H, Razmovski-Naumovski V, Kyada AV, Parekh HS, Li GQ, Nammi S. Myoglobin and low density lipoprotein glycation in cardiac complications of diabetes: Current perspectives and therapeutic options. Indian J Med Res. 2015. In Press. Jadeja R, Devkar RV, Nammi S. Hepatoprotective potential of herbal medicine. Evid-Based Compl Alt. [Editorial]. 2015;2015.. Kam A, Li KM, Razmovski-Naumovski V, Nammi S, Chan K, Grau GE, et al. Curcumin reduces tumour necrosis factor-enhanced annexin V-positive microparticle release in human vascular endothelial cells. J Pharm Pharm Sci. 2015;18(4):424-33.. Thounaojam MC, Nammi S, Jadeja R. Natural products for the treatment of obesity, metabolic syndrome, and type 2 diabetes 2014. Evid-Based Compl Alt. [Editorial]. 2015;2015. DOI: 10.1155/2015/392681.. Yang WC, Chang CLT, Li CR, Nammi S, Cho WCS. Complementary and alternative medicine for diabetes 2014. Evid-Based Compl Alt. [Editorial]. 2015;2015. DOI: 10.1155/2015/685248.. 2014. Li Y, Tran VH, Kota BP, ...
Abcam provides specific protocols for Anti-Annexin A10 antibody (ab2343) : Western blot protocols, Immunohistochemistry protocols
Annexin A1兔多克隆抗体(ab65844)可与小鼠, 大鼠, 人样本反应并经WB, IP, IHC, ICC实验严格验证。所有产品均提供质保服务,中国75%以上现货。
Annexin A2兔多克隆抗体(ab90643)可与人样本反应并经WB, IHC实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
What medication is light yellow round has V on one side and 2530 and a score line on the other side The KGB Agent answer: Not Medical Advice: Pill imprint 2530 V has been identified as Clonazepam 0.5 mg. It is used in the treatment of anxiety, bipolar disorder and more.
Caveolin-1 (Cav-1), a family of ubiquitously expressed oligomeric structural proteins in many mammalian cells, has been shown to be an effective regulator of tumorigenesis. Recent studies have indicated that Cav-1 can promote resistance to chemotherapy in a variety of tumors. However, the regulation of Cav-1 on chemoresistance in ovarian cancer is still unknown. In the present study, the mRNA and protein expression level was investigated by RT-PCR and western blot analysis, respectively, and the 50% inhibitory concentration (IC50) value was measured by MTT assay. The protein expression level of P-glycoprotein (P-gp), Notch-1, p-Akt and p-NF-κB p65 were detected using western blot analysis and the apoptotic ratio was determined using the Annexin V-FITC/PI detection kit. The results showed that the mRNA and protein expression levels of Cav-1 were significantly higher in SKOV3/DDP and A2780/DDP than in SKOV3 and A2780, respectively. Knockdown of Cav-1 significantly decreased the IC50 value in ...
Mouse Monoclonal Anti-Annexin A3 Antibody (2F3). Validated: WB, ICC/IF, IHC, IHC-P. Tested Reactivity: Human, Monkey. 100% Guaranteed.
Tinción con anexina V y propidio yoduro (PI) ofrece a los investigadores una manera de identificar distintos tipos de muerte celular:...
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STUDY QUESTION Are circulating microparticles (MPs) altered in young women with polycystic ovary syndrome (PCOS)? SUMMARY ANSWER Women with PCOS have elevated concentrations of circulating platelet-derived MPs, which exhibit increased annexin V binding and altered microRNA (miR) profiles compared with healthy volunteers. WHAT IS KNOWN ALREADY Some studies have shown that cardiovascular risk is increased in young women with PCOS but the mechanisms by which this occurs are uncertain. Circulating MPs are elevated in patients with cardiovascular disease but the characteristics of MPs in patients with PCOS are unclear. STUDY DESIGN, SIZE, DURATION Case-control study comprising 17 women with PCOS (mean ± SD; age 31 ± 7 years, BMI 29 ± 6 kg/m2) and 18 healthy volunteers (age 31 ± 6 years, BMI 30 ± 6 kg/m2). PARTICIPANTS/MATERIALS, SETTING, METHODS The study was conducted in a University hospital. Nanoparticle tracking analysis (NTA) and flow cytometry (CD41 platelet, CD11b monocyte, CD144 ...
In our hands, the trypsin actually reduced the Annexin staining presumably by stripping off all the phosphotidyl-serine on the surface along with the surface proteins (adhesion molecules). The only time we have seen enhanced annexin is when the cells suffer enough trauma in harvesting that the membrane is not intact and the Annexin gets inside the cell and attaches to the ps on the inner membrane. Julie At 02:11 PM 8/18/98, you wrote: , ,I have a client who is staining with Annexin-FITC. They are looking at ,cell lines and are using trypsin to get the cells off the flasks. We are ,seeing what we think is an exagerated Annexin response. Does anyone out ,there have any feedback on how trypsin affects Annexin staining? Any ,experience and or information about this will be greatly appreciated. , Thanks in advance, , Joan Kalnitsky ,Joan Kalnitsky ,Flow Cytometry Laboratory ,VMRCVM ,(540) 231-4115 ,FAX 540-231-7367 , , It is better to serve than to receive , B. Borg , , , , , ...
Membrane repair is essential to cell survival. In skeletal muscle, injury often associates with plasma membrane disruption. Additionally, muscular dystrophy is linked to mutations in genes that produce fragile membranes or reduce membrane repair. Methods to enhance repair and reduce susceptibility to injury could benefit muscle in both acute and chronic injury settings. Annexins are a family of membrane-associated Ca2+-binding proteins implicated in repair, and annexin A6 was previously identified as a genetic modifier of muscle injury and disease. Annexin A6 forms the repair cap over the site of membrane disruption. To elucidate how annexins facilitate repair, we visualized annexin cap formation during injury. We found that annexin cap size positively correlated with increasing Ca2+ concentrations. We also found that annexin overexpression promoted external blebs enriched in Ca2+ and correlated with a reduction of intracellular Ca2+ at the injury site. Annexin A6 overexpression reduced membrane ...
Annexins are a structurally related family of Ca2+ binding proteins of undertermined biological function. Annexin I (also called lipocortin 1) is a substrate for the EGF-stimulated tyrosine kinase and is postulated to be involved in mitogenic signal transduction. To investigate further the involvement of lipocortin 1 in cell proliferation, we measured lipocortin 1 levels in normal diploid human foreskin fibroblasts (HFF) to determine whether its expression changed as a function of growth status. For comparison, the expression of annexin V (also called endonexin II) was measured in HFF cells. Endonexin II is a protein with similar Ca2+ and phospholipid binding properties as lipocortin 1, but it is not a substrate for tyrosine kinases. Quiescent HFF cell cultures were induced to proliferate by either subculture to lower cell density, EGF stimulation, or serum stimulation. In all three protocols, proliferating HFF cells contained three- to fourfold higher levels of lipocortin 1 and three- to ...
CEA in Pancreatic cyst fluid FGF-23 ( Fibroblast growth Factor-23) Anti-Mullerian Hormone ( AMH) Inhibin-B Anti-Annexin V(IgG and IgM) Vitamin D
Animals. Wild-type mice from the 129T2/SvEmsJ background were bred and housed in a specific pathogen-free facility on a 12-hour light/12-hour dark cycle and fed ad libitum in accordance with the Northwestern Universitys Institutional Animal Care and Use Committee regulations. 129T2/SvEmsJ (129T2) mice were purchased from the Jackson Laboratory (stock 002065). mdx/hLTBP4 mice were generated as described previously (34, 60). Sgcg-null mice were generated as described in Hack et al. (46). Two- to 3-month-old males and females were used for all wild-type mouse experiments. Sgcg-null cohorts were age and sex matched with mice between 2 and 5 months old.. Plasmids. Plasmids encoding annexin A1, A2, and A6 with a carboxyl-terminal turboGFP tag were obtained from Origene. Subcloning of annexin A1, A2, and A6 to replace the GFP tag with tdTomato (Addgene) was performed by Mutagenix. Site-directed mutagenesis was performed by Mutagenix on annexin A1-GFP, A2-GFP, and A6-GFP to create the Ca2+-binding ...
Animals. Wild-type mice from the 129T2/SvEmsJ background were bred and housed in a specific pathogen-free facility on a 12-hour light/12-hour dark cycle and fed ad libitum in accordance with the Northwestern Universitys Institutional Animal Care and Use Committee regulations. 129T2/SvEmsJ (129T2) mice were purchased from the Jackson Laboratory (stock 002065). mdx/hLTBP4 mice were generated as described previously (34, 60). Sgcg-null mice were generated as described in Hack et al. (46). Two- to 3-month-old males and females were used for all wild-type mouse experiments. Sgcg-null cohorts were age and sex matched with mice between 2 and 5 months old.. Plasmids. Plasmids encoding annexin A1, A2, and A6 with a carboxyl-terminal turboGFP tag were obtained from Origene. Subcloning of annexin A1, A2, and A6 to replace the GFP tag with tdTomato (Addgene) was performed by Mutagenix. Site-directed mutagenesis was performed by Mutagenix on annexin A1-GFP, A2-GFP, and A6-GFP to create the Ca2+-binding ...
White Round Pill 4810 V On One Side - Topics (Showing results 1 - 20 of 27)... round white pill v 48 10: I do not know what this is and no site has it listed help me ## Oxycodone 5mg/ no APAP( no tylenol a... picture of a oxycodone small white round pill and on other side has an x: small round white pill ## i been usin
Purpose: : The use of immortalized HCEC currently is widespread among cell death and gene transfer vision research investigators. This study evaluates characteristics of primary versus immortalized HCEC in early apoptosis and the response of these cells to gene transfer with empty vector (IZsGW, GFP) and the anti-apoptotic gene Bcl-xL. Methods: : Apoptosis was induced in immortalized and primary (passage 8) HCEC with an external (Actinomycin) and an internal (Etoposide) apoptotic inducer (0.1-100 micrograms/ml for 2-120h). Sensitivity towards Lentivirus and Adenovirus was examined using pHAGE-CMV-MCS-IZsGW (1.5 x 10^8 IU/ml) and AAV2-GFP (3.2 x 10^12 IU/ml, 3.2 x 10^3- 3.2 x 10^8; 24 hours of incubation, days 1-23). Flow cytometry was used to detect apoptosis with Annevin V-FITC and Propidium Iodide as well as expression rates of IZsGW and GFP. Apoptosis in Lenti-IZsGW-Bcl-xL versus Lenti-IZsGW (as empty vector, EV) was detected with Annexin V-PE, 7-AAD and flow cytometry. Results: : Early ...
Two-dimensional crystals of annexin-V bound to lipid layers containing dioleoylphosphatidylserine have been obtained in the presence of Ca2+. The crystals diffract to 20 A resolution and have the symmetry of the plane group p3 (unit cell dimensions: a = b = 94 A, gamma = 120 degrees). Electron image analysis revealed that the crystals are composed of trimers of annexin-V forming triskelion-like motifs. Each annexin-V molecule has a characteristic elongated shape, about 65 A by 20 A, when observed perpendicularly to the crystal plane. It is composed of two staggered domains of similar size, about 40 A by 20 A. Both domains are made of two sub-domains. The present data suggest that the four resolved sub-domains represent the folding units corresponding to the four 70 amino acid repeating segments characteristic of all annexins.
Page contains details about annexin V conjugated to maleimide-functionalized Feraheme nanoparticles . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Annexin A5 is a biomarker used in Hiv Infection, Acute Lymphoblastic Leukemia, Hypertension and 942 other diseases. Learn more about Annexin A5.
B) HCT116 cells were treated with increasing doses of SN-38 and treated with 4 nM SN-38 for different time. Cell extracts were prepared and analyzed by Western blotting with indicated antibody. These experiments were repeated thrice. (C) HCT116 cells were transfected with 2 μg of EGFP-LC3 construct. At 8 h post-transfection, cells were treated with 4 nM of SN-38 for 48 h. And then cells were examined by confocal microscopy (magnification × 400). The percentage of cells showing accumulation of EGFP-LC3 in puncta (EGFP-LC3vac) was quantified. (D) LOVO and HCT116 cells were treated with 2 nM and 4 nM of SN-38 combined with 10 mM of 3-Methyladenine (3-MA) for 48 h respectively. Cell extracts were prepared and analyzed by Western blotting with indicated antibody. These experiments were repeated thrice. (E) Cells were treated with indicated concentrations of 3-MA and SN-38 for 48 h. Cell apoptosis was assessed by Annexin V-FITC/PI staining assay by flow cytometry. Columns, means of three ...
Oncotarget | https://doi.org/10.18632/oncotarget.23914 Teruo Yamauchi, Jose Rodrigo Espinosa Fernandez, Chiyo K. Imamura, Hideko Yamauchi, Hiromitsu Jinno, Maiko Takahashi, Yuko Kitagawa, Seigo Nakamura, Bora...
Taylor, SRJ, Gonzalez-Begne, M, Dewhurst, S, Chimini, G, Higgins, CF, Melvin, JE and Elliott, JI (2008) Sequential shrinkage and swelling underlie P2X(7)-stimulated lymphocyte phosphatidylserine exposure and death ...
Annexin A11 Antibody 10479-2-AP has been identified with IF, IHC, IP, WB, ELISA. 10479-2-AP detected 56-60 kDa band in HeLa cells with 1:500-1:2000 dilution...
Annexin A1 Antibody 66344-1-Ig has been identified with ELISA, IF, IHC, WB. 66344-1-Ig detected 35 kDa band in A431 cells with 1:2500-1:10000 dilution...
Complete information for PF4V1 gene (Protein Coding), Platelet Factor 4 Variant 1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
2. The last i checked this was not true, but just in case.... I am not using Pins 2 or 3 (the data pins on the USB) at all because to my knowledge, droids just need 5v on pin 1 and 0v on pin 4 to charge (as opposed to apple devices that requires a data signal in order to charge). Is this the same for droids now too? do you think I have to wire the data pins in ...
Sigma-Aldrich offers abstracts and full-text articles by [Jiangnan Li, Dongwei Guo, Li Huang, Manman Yin, Qingfang Liu, Yan Wang, Chunmei Yang, Yuanyuan Liu, Lijie Zhang, Zhijun Tian, Xuehui Cai, Liyun Yu, Changjiang Weng].
From Softwares announced its releasing Armored Core V on January 26 for ¥7,800 in Japan on PS3 and Xbox 360. The mech title was suposed to lau…
Found this little egret with coloured rings as it stood in the main pool in the parks grazing fields on Tuesday 8th. It appeared that the red ring had the letter V on it but couldnt make out if there was a letter on the green ring. There is a ringing programme currently taking place in England, so it will be interesting to find out where this bird was ringed as a chick. The details have been sent off ...
For the MC question with 8 graphs (picking the two for charging a capacitor I believe? with C/V on one y-axis and I (Current) on the other? I put the one with the C/V graph going up but the rate decreasing exponentially and the amplitude graph going down exponentiall (Becuase I think current always decreases with capacitors) Is this right ...
Apoptosis, or programmed cell death, is a general mechanism for removal of unwanted cells from the immune system. It is characterized by chromatin condensation, a reduction in cell volume, and endonuclease cleavage of DNA into oligonucleosomal length fragments. Apoptosis is also accompanied by a loss of membrane phospholipid asymmetry, resulting in the exposure of phosphatidylserine at the surface of the cell. Expression of phosphatidylserine at the cell surface plays an important role in the recognition and removal of apoptotic cells by macrophages. Here we describe a new method for the detection of apoptotic cells by flow cytometry, using the binding of fluorescein isothiocyanate-labeled annexin V to phosphatidylserine. When Burkitt lymphoma cell lines and freshly isolated germinal center B cells are cultured under apoptosis inducing conditions, all cells showing chromatin condensation strongly stain with annexin V, whereas normal cells are annexin V negative. Moreover, DNA fragmentation is ...
Annexin A1 is an intracellular calcium/phospholipid-binding protein that is involved in membrane organization and the regulation of the immune system. It has been attributed an anti-inflammatory role at various control levels, and recently we could show that annexin A1 externalization during secondary necrosis provides an important fail-safe mechanism counteracting inflammatory responses when the timely clearance of apoptotic cells has failed. As such, annexin A1 promotes the engulfment of dying cells and dampens the postphagocytic production of proinflammatory cytokines. In our current follow-up study, we report that exposure of annexin A1 during secondary necrosis coincided with proteolytic processing within its unique N-terminal domain by ADAM10. Most importantly, we demonstrate that the released peptide and culture supernatants of secondary necrotic, annexin A1-externalizing cells induced chemoattraction of monocytes, which was clearly reduced in annexin A1- or ADAM10-knockdown cells. Thus, ...
Annexin A2 is a calcium-dependent, phospholipid-binding protein found on many cell types. It consists of a short hydrophobic tail (Ser2-Asn32), which dictates its function, and a core domain (Phe33-Asp339), which is involved in phospholipid binding. Annexin A2 has been implicated in a number of biochemical processes, including cell proliferation, foetal immune tolerance, ion-channel activation, cell-cell interactions and the bridging of membranes. Annexin A2 is reported to be a powerful activator of plasminogen and, therefore, is implicated in many normal and pathological processes such as haemostasis and metastasis. Myeloid cell lines are used, extensively, to study many aspects of cellular proliferation, differentiation and function. In the present study, we have used flow cytometry and real-time PCR to investigate the role of annexin A2 expression in the proliferation and differentiation of a number of myeloid cell lines. The results demonstrated that annexin A2 expression was affected when ...
Order monoclonal and polyclonal Annexin VIII antibodies for many applications. Selected quality suppliers for anti-Annexin VIII antibodies.
In the present study, we show that both CRP and annexin A5 specifically bind to oxidized LDL. Via coupling to Fcγ receptors on macrophages and independent of complement, CRP subsequently enhances the association of oxidized LDL to macrophages. Enhanced association of oxidized LDL to macrophages via CRP-Fcγ receptor interaction may lead to enhanced macrophage uptake of oxidized LDL. Moreover, we show that annexin A5 binds to a site at oxidized LDL different from the CRP binding site and that annexin A5 does not antagonize the CRP effect.. Previously, Chang et al also observed specific binding of CRP to oxidized LDL,7 characterized by inhibition by whole molecules as well as F(ab′)2 fragments of EO6, an IgM antibody that binds specifically to the phosphorylcholine head group of oxidized phospholipids but not to the same moiety on nonoxidized phospholipids.26 In the present study, for characterization of binding sites, we used the monoclonal antibody DLH3 (also IgM), which recognizes an epitope ...
The Annexin family of calcium-binding proteins is composed of al least thirteen mammalian genes (Annexin A1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. The Annexin family
Bax (Apoptosis Marker) Antibody - With BSA and Azide, Purified Mouse Monoclonal Antibody validated in WB, IHC, IF, FC, IP, E (AH10026-20), Abgent
Bcl-X (Apoptosis Marker) Antibody - Without BSA and Azide, Mouse Monoclonal Antibody [Clone 2H12 ] validated in WB, IHC-P, IF, FC (AH10725-100), Abgent
Phosphatidylserine, a phospholipid with a negatively charged head-group, is an important constituent of eukaryotic cellular membranes. On the plasma membrane, rather than being evenly distributed, phosphatidylserine is found preferentially in the inner leaflet. Disruption of this asymmetry, leading to the appearance of phosphatidylserine on the surface of the cell, is known to play a central role in both apoptosis and blood clotting. Despite its importance, comparatively little is known about phosphatidylserine in cells: its precise subcellular localization, transmembrane topology and intracellular dynamics are poorly characterized. The recent development of new, genetically-encoded probes able to detect phosphatidylserine within live cells, however, is leading to a more in-depth understanding of the biology of this phospholipid. This review aims to give an overview of the current methods for phosphatidylserine detection within cells, and some of the recent realizations derived from their use.
Results Number (control MP: 3.0*1010/mL vs PIC-induced MP: 3.1*1010/mL; n=2), size (median diameter 207 vs 199nm, n=2), Annexin V binding (mean ± SD: 66±10% vs 63±9%, n=3), the presence of CD4 on MP (20.9±1.4% vs 21.7±3.4%) and total protein content (330±50 vs 325±83 ng/mL) did not differ significantly between control MP and PIC-induced MP. FITC PIC was detected in 14.5±1.3% of U937-derived MP (n=3). In contrast to soluble PIC, MP-associated FITC-PIC was resistant to degradation by RNAse III (FITC-PIC MP + RNaseIII: 33.3±0.7; FITC-PIC MP: 29.0±2.6, n=2-3) and was stably present in cell culture supernatants from RASF treated with MP for 24h (FITC-PIC MP: 12.9 ± 0.2, n=3). PIC was transferred to RASF by MP as confirmed by a 27±15% increase in fluorescence intensity of RASF (n=7, p=0.002) treated with FITC PIC-induced vs PIC-induced MP. The sensitivity of RASF to TRAIL-induced apoptosis decreased after treatment of RASF with PIC-induced MP but not control MP, as shown by inhibition of ...
OBJECTIVE: To investigate the mechanism of reversing drug resistance of K562/D cells to daunorubicin by Embelin and its relationship with P-gp and MDR1 mRNA. METHODS: MTT assay was used to detect and compare the cell proliferation rate of treating with DNR alone and DNR combined with Embelin. Flow cytometry with Annexin V-FITC/PI double staining was used to detect cell apoptosis rate, Western blot was used to detect the expression of XIAP,Caspase-3,BCL-2,BAX and P-gp of K562/D cells after using DNR alone and combining with Embelin ...
SCAN HISTORY OWL11_0 2 100 NSINGLE OWL17_1 2 110 NSINGLE OWL18_0 1 115 NSINGLE OWL19_1 1 125 NSINGLE OWL21_1 1 125 NSINGLE OWL26_0 1 125 NSINGLE SPTR37_9f 2 237 NSINGLE INITIAL MOTIF SETS ANNEXIN1 Length of motif = 23 Motif number = 1 Annexin motif I - 1 PCODE ST INT KTKGVDEVTIVNILTNRSNAQRQ LUHU36 46 46 KTKGVDEVTIINILTNRSNEQRQ ANX2_CHICK 46 46 TVKGVDEATIIDILTKRNNAQRQ ANX1_CAVCU 56 56 MVKGVDEATIIDILTKRNNAQRQ LUHU 55 55 KGIGTDEATIIDIVTHRSNAQRQ ANX6_MOUSE 376 376 KGMGTDEETILKILTSRNNAQRQ ANX5_CHICK 29 29 KGIGTNEQAIIDVLTKRSNTQRQ ANX8_HUMAN 35 35 KGFGTDEQEIIDVLVGRSNQQRQ DROANNX 29 29 RGIGTDEKMLISILTERSNAQRQ ANX3_HUMAN 32 32 KGLGTDEDAIINVLAYRSTAQRQ ANX4_BOVIN 27 27 KGFGTDEQAIVDVVANRSNDQRQ HUMSNEXIN 177 177 TAKGVDEATIIDIMTTRTNAQRP ANX1_COLLI 51 51 ANNEXIN2 Length of motif = 17 Motif number = 2 Annexin motif II - 1 PCODE ST INT LKSALSGHLETVILGLL LUHU36 86 17 LKSALSGHLEAVILGLL ANX2_CHICK 86 17 LKKALTGHLEEVVLALL ANX1_CAVCU 96 17 LKKALTGHLEEVVLALL LUHU 95 17 LKSEISGDLARLILGLM ANX6_MOUSE 416 17 ...
Buy ANXA2 recombinant protein, Annexin A2 Recombinant Protein-NP_001002857 (MBS203687) product datasheet at MyBioSource, Recombinant Proteins. Application: SDS-PAGE
AAD positive means late apoptosis? - posted in Flow Cytometry: We have a discussion in the lab about apoptosis detection by annexin V/AAD labeling. Many people say that Annexin V+/AAD+ cells must not be considered apoptotic but necrotic. I agree, provided that the apoptotic stimulus was rather short (for example 2 hours-staurosporine). However, when the stimulus lasts for 24 hours (serum starvation for instance) I think that cells which started the apoptotic process shortly after the start...
Sluyter, R., Shemon, A. & Wiley, J. S. (2007). P2X7 receptor activation causes phosphatidylserine exposure in human erythrocytes. Biochemical and Biophysical Research Communications, 355 169-173 ...
Bachem offers H-3196 Annexin A1 (1-25) (dephosphorylated) (human) for your research. Find all specific details here. Find product specific information including available pack sizes, CAS, detailed description and references here.
If the V on Michaels thriller jacket stands for what these new agers etc call the 5th age/dimention that either starts or ends(depending on what you read) on 21-23/12/12 then Michael was trying to alert us to the date being when the beast is revealed in the thriller video also. In the short film, in the cinema scene at the begining, after the girl gets up and leaves a conversation can be heard discussing the beast and a note written in blood that the beast has left. One voice asks what does it say.At which point Michael stands up to leave. The reply is see you next wednesday. (midst of the week) At this point Michael turns his back to the screen showing the V on his back which if it is refering to the 5th age Michael was telling us there also the middle of the week is 21-23/12/12 when the beast is revealed ...
am jose.I dont no how to tell the word the greatest thing that happen in my life.I was so sick that i loose all the money I have in so many hospitals still yet there was no solutions on till I go for blood test for different hospital almost five doctors told me I am H.I.V positive. & after then all hope I have was lost I was waiting the day lord will called me. One nite I woke up in mind-nite start crying so my mind told me to check in Google so I see many testimony about dr okosun I was daunting if the man is real a great man but just because of the testimony of his good news.I said let me try & I email him he reply me back I told him my problems he said that he will reply me back three minis time after he check the oracle. So he reply me after three minis & told me the doctors were saying the truth of my blood test. He said it was spiritual H.I.V. I was having that he can be able to help me. So I respond to all the thins he ask me to do. after then I go for a checkup in three different ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Annexin A2 is a calcium regulated protein involved in membrane binding and actin polymerization. In this paper, Kevin Moreau and colleagues identified Annexin A2 as a…. ...
hi, ok i know that the pill i have is hydrocodone/acetaminophen... its generic for vicodin... it has a V on one side and a 35 92 on the other. my
www.ncbi.nlm.nih.gov/pmc/articles/PMC3063939. Our results indicate that 4-1BB co-stimulation may significantly improve TIL survival during ... mAb from Bristol Myers Squibb (BMS) currently being tested in clinical trials,. ... BMS-663513 was at 14.9 mg/ml and was stored at 4°C. This Ab was .... The cells were then stained with anti-CD8-Pacific Blue, Annexin V-PE, and .... ...
Its an unimaginable tragedy. Two young boys killed while on a sleepover at a friends apartment. Authorities say they were suffocated by a 100-pound snake. The situation is heartbreaking. Tonight, there is a criminal investigation underway.
Mouse anti Human Annexin A3 antibody, clone 4F1 recognizes human annexin A3, also known as Annexin III, Inositol 1,2-cyclic phosphate 2-ph
Background: The neutral comet assay was devised to measure double-stranded DNA breaks, but it has also been used to measure apoptosis based on its characteristic DNA fragmentation patterns. There is still uncertainty about the reliability of this method. By comparing the comet assay with a flow cytometry method that uses Annexin V binding to apoptotic cells, we have provided further evidence for evaluating the usefulness of the comet assay for detecting apoptosis. Methods: Apoptosis was induced in human peripheral blood mononuclear cells (PBMC) by ionizing radiation and measured using the comet assay and a flow cytometry method that measures Annexin V and propidium iodide (PI) staining. Results: The Annexin V flow cytometry assay distinguished among early apoptosis, late apoptosis, and an apoptotic or necrotic phase in which the cells were labeled with both Annexin V and PI. The comet assay detected only the latter two phases of apoptosis. Conclusions: The comet assay is a useful tool for ...
Purpose: : To examine the role of annexin 2 in the formation and internalisation of phagosomes in retinal pigment epithelial (RPE) cells. Methods: : Human ARPE19 cells were cultured on the glass inserts of Matek dishes for immunofluorescence and live imaging, or plastic Nunc dishes for biochemical analysis. In some studies ARPE19 cells were exposed to siRNA for annexin 2 for three days prior to experimentation. Outer segments were prepared from porcine retina using standard protocols (typically 50 - 100 eyes per prep), and either used fresh in biochemical experiments, or labelled with an appropriate fluorescent marker prior to imaging experiments. Results: : Depletion of annexin 2 using siRNA led to a significant quantitative reduction in the phagocytosis of rod outer segments in cultured RPE cells. In cells expressing annexin 2, imaged by confocal microscopy, a striking enrichment of annexin 2 and F-actin was observed at the phagocytic cup immediately upon engagement of the apical cell surface ...
Annexin A1, also known as lipocortin I, is a protein that is encoded by the ANXA1 gene in humans. Annexin A1 belongs to the annexin family of Ca2+-dependent phospholipid-binding proteins that have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin A1 protein has an apparent relative molecular mass of 40 kDa with phospholipase A2 inhibitory activity. Glucocorticoids (such as budesonide, cortisol, and beclomethasone) are a class of endogenous or synthetic anti-inflammatory steroid hormones that bind to the glucocorticoid receptor (GR), which is present in almost every vertebrate animal cell. They are used in medicine to treat diseases caused by an overactive immune system, including allergies, asthma, autoimmune diseases, and sepsis. Because they suppress inflammatory pathways, long-term use of glucocorticoid drugs can lead to side-effects such as immunodeficiency and adrenal insufficiency. The main mechanism of ...
What is the pill thats blue round has a V on one side m 2101 on other side The KGB Agent answer: Not Medical Advice A round blue pill with the imprint V on the front, and 2101 on the back of it is 10mg of Amitriptyline hydrochloride (made by Qualitest Pharmaceuticals). Amitriptyline hydrochloride is a tricyclic antidepressant that is used to treat chronic myofascial pain, insomnia, pain, burning mouth syndrome, and anxiety and stress, among other illnesses. It is also sold under the brand names: • Elavil • Vanatrip There is a low risk of abuse with this drug. This prescription-only medication may pose a risk to an unborn fetus, and should be used only under a doctors supervision. WebMD offers their 10 Tips to Beat Insomnia, PsychCentral provides 11 Tips to Help Manage Anxiety, and Readers Digest offers their 37 Stress Management Tips from the Experts. The Standard-Examiner is reporting that Weight gain and antidepressants are a big issue.
TY - JOUR. T1 - Assessment of secondary necrosis of Jurkat cells using a new microscopic system and double staining method with annexin V and propidium iodide.. AU - Honda, O.. AU - Kuroda, Masahiro. AU - Joja, I.. AU - Asaumi, Jun-Ichi. AU - Takeda, Yoshihiro. AU - Akaki, S.. AU - Togami, I.. AU - Kanazawa, Susumu. AU - Kawasaki, S.. AU - Hiraki, Y.. PY - 2000/2. Y1 - 2000/2. N2 - Using a new system developed by us for acquiring microscopic images automatically, we compared the morphological changes that apoptotic cells undergo with changes in the staining pattern of annexin V-enhanced green fluorescent protein (AV-EGFP) and propidium iodide (PI) in individual cells. Jurkat cells were treated with 5 mM CaCl2 alone, anti-Fas antibody and heating at 42 degrees C for 30 min or 46 degrees C for 60 min, and then were incubated in medium with 5 mM CaCl2. Time-lapse DNA fragmentation analysis and morphological observation revealed that the anti-Fas antibody and heating at 42 degrees C for 30 min ...
TransDetect® Annexin V-EGFP/PI Cell Apoptosis Detection Kit,Cell Detection,Cell Culture and Detection,Products,Beijing TransGen Biotech Co.Ltd,OverviewContents& storageCitations & referencesRelated ImagesDownloadOverviewDescriptionThe Annexin V
Annexin A1 / ANXA1, 0.1 ml. Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane.
Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. The alternative splicing event is tissue specific and the mRNA containing the cassette exon is prevalent in brain, heart and skeletal muscle. The transcripts also differ in their 3-non coding regions by the use of two alternative poly(A) signals. Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, ...
KINGSTON, J. K., BAYLY, W. M., MEYERS, K. M., SELLON, D. C. and WARDROP, K. J. (2002), Evaluation of binding of fibrinogen and annexin V to equine platelets in response to supramaximal treadmill exercise. Equine Veterinary Journal, 34: 502-505. doi: 10.1111/j.2042-3306.2002.tb05473.x ...
Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 has 45 to 59% identity with other members of its family and shares a similar size and exon-intron organization. Isolated from human placenta, ANX4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells ...
Background 17-Estradiol (E2) has been reported to protect annulus fibrosus (AF) cells against interleukin-1 (IL-1)-induced apoptosis in a concentration-dependent manner. Material and Methods Reagents We used the following reagents: DMEM/F12 (Gibco, USA), fetal bovine serum (FBS) (BI, Israel), trypsin (Sigma, USA), collagenase type II (Sigma, USA), D-Hanks and PBS (Solarbio, Beijing, China), Annexin V-FITC/PI kit (BD, USA), primary antibody of anti-1 (Proteintech, Wuhan, China), E2 (Sigma, USA), collagen II (Sigma, USA), ICI182780 (Sigma, United Kingdom), and secondary antibody (goat anti-rabbit) (Proteintech, Wuhan, China). Ethical statement The protocol for animal use in these experiments was approved by the Institutional Review Gata3 Board of the Affiliated Taizhou Peoples Hospital of Nantong University. Cell culture protocol Annulus fibrosus cells were isolated from male Wistar rats (~200 g) using the culture methodology reported previously [19]. In brief, 3 male Sprague-Dawley rats were ...
This video demonstrates the use of the Tali® Apoptosis Kit with the Tali® Image-Based Cytometer. The Tali® Apoptosis Kit enables identification of apoptotic cells and discrimination of apoptotic from necrotic and live cells in a population. The kit stains apoptotic cells with green Annexin V -- Alexa Fluor® 488, stains necrotic cells with both red propidium iodide and green Annexin V -- Alexa Fluor® 488, and does not stain live cells. The Tali® Image-Based Cytometer allows for quick, quantitative analysis of apoptosis, cell viability, and much more right from your benchtop.. ...
TY - JOUR. T1 - The membrane phospholipid binding protein annexin A2 promotes phagocytosis and nonlytic exocytosis of cryptococcus neoformans and impacts survival in fungal infection. AU - Stukes, Sabriya. AU - Coelho, Carolina. AU - Rivera, Johanna. AU - Jedlicka, Anne E.. AU - Hajjar, Katherine A.. AU - Casadevall, Arturo. PY - 2016/8/15. Y1 - 2016/8/15. N2 - Cryptococcus neoformans is a fungal pathogen with a unique intracellular pathogenic strategy that includes nonlytic exocytosis, a phenomenon whereby fungal cells are expunged from macrophages without lysing the host cell. The exact mechanism and specific proteins involved in this process have yet to be completely defined. Using murine macrophages deficient in the membrane phospholipid binding protein, annexin A2 (ANXA2), we observed a significant decrease in both phagocytosis of yeast cells and the frequency of nonlytic exocytosis. Cryptococcal cells isolated from Anxa2-deficient (Anxa2-/-) bone marrow-derived macrophages and lung ...
Gliomas are highly invasive brain tumors with the occurrence of numerous microglia/macrophages (MG/MP) in and around the tumor. Annexin A2 is overexpressed in many cancers and correlates with increased plasmin activity on the tumor cell surface. Plasmin mediates degradation of extracellular matrix and angiogenesis to facilitate tumor growth. In this study, we used a mouse glioma cell line, GL261-EGFP, and stable clones transfected with an annexin A2 knockdown (annA2KD) construct, GL261-EGFP-annA2KD. We found that the annA2KD decreased glioma cell migration in vitro and decreased membrane-bound plasmin activity. In vivo we injected GL261-EGFP cells into the mouse brain and the glioma progression was followed. Knockdown of annexin A2 in glioma cells decreased tumor size and slowed down tumor progression, characterized by decreased invasion, angiogenesis and proliferation, as well as increased apoptosis in tumor tissue of the annA2KD group. We also investigated the interaction between glioma and ...
TY - JOUR. T1 - Annexins family. T2 - Insights into their functions and potential role in pathogenesis of sarcoidosis. AU - Mirsaeidi, Mehdi. AU - Gidfar, Sanaz. AU - Vu, Ann. AU - Schraufnagel, Dean. PY - 2016. Y1 - 2016. N2 - Annexins are Ca2+-regulated phospholipid-binding proteins that play an important role in the cell life cycle, exocytosis, and apoptosis. Annexin A11 is one of the oldest vertebrate annexins that has a crucial role in sarcoidosis pathogenesis. The mechanism of effect in sarcoidosis granuloma cells may be due to alterations in apoptosis. Immune cells with a specific mutation at protein location 230 are resistant to apoptosis and consequently have continued effects on inflammation and progression of sarcoidosis. The mechanism of action of annexin A11 may be based upon alterations in delivering calcium to two different apoptosis pathways (caspase and P53).. AB - Annexins are Ca2+-regulated phospholipid-binding proteins that play an important role in the cell life cycle, ...
Manipulations in cell culture and mouse models have demonstrated that reduction of collagen V results in altered fibril structure and matrix assembly. A tissue-dependent role for collagen V in determining mechanical function was recently established, but its role in determining regional properties has not been addressed. The objective of this study was to define the role(s) of collagen V expression in establishing the site-specific properties of the supraspinatus tendon. The insertion and midsubstance of tendons from wild type, heterozygous and tendon/ligament-specific null mice were assessed for crimp morphology, fibril morphology, cell morphology, as well as total collagen and pyridinoline cross-link (PYD) content. Fibril morphology was altered at the midsubstance of both groups with larger, but fewer, fibrils and no change in cell morphology or collagen compared to the wild type controls. In contrast, a significant disruption of fibril assembly was observed at the insertion site of the null ...
The Lymphatic Fluid and T-Cell Receptor Vβ expression in Peripheral Blood Mononuclear Cells (PBMCs) of patients with Wuchereria Bancrofti Infection - Dr. Magapu Solomon Sudhakar - Doctoral Thesis / Dissertation - Medicine - Biomedical Engineering - Publish your bachelors or masters thesis, dissertation, term paper or essay

Annexin A5 - WikipediaAnnexin A5 - Wikipedia

Annexin A5 (or annexin V) is a cellular protein in the annexin group. In flow cytometry, annexin V is commonly used to detect ... Annexin A5 showed upregulation in papillary thyroid carcinoma. Annexin A5 is used as a non-quantitative probe to detect cells ... Annexin A5 forms a shield around negatively charged phospholipid molecules. The formation of an annexin A5 shield blocks the ... The annexin A5 affinity assay typically uses a conjugate of annexin V and a fluorescent or enzymatic label, biotin or other ...
more infohttps://en.wikipedia.org/wiki/Annexin_A5

Annexin A3 - WikipediaAnnexin A3 - Wikipedia

Annexin A3 is a protein that in humans is encoded by the ANXA3 gene. It is abnormally expressed in fetuses of both IVF and ICSI ... "Entrez Gene: ANXA3 annexin A3". Zhang Y, Zhang YL, Feng C, et al. (September 2008). "Comparative proteomic analysis of human ... 2005). "Annexin A3 is a potential angiogenic mediator". Biochem. Biophys. Res. Commun. 337 (4): 1283-7. doi:10.1016/j.bbrc. ... "The high-resolution crystal structure of human annexin III shows subtle differences with annexin V". Biochemistry. 35 (6): 1740 ...
more infohttps://en.wikipedia.org/wiki/Annexin_A3

annexin A5 - Semantic Scholarannexin A5 - Semantic Scholar

A protein of the annexin family isolated from human PLACENTA and other tissues. It inhibits cytosolic PHOSPHOLIPASE A2, and ... Annexin A5, human. diannexin, human. technetium 99m tricarbonyl His-annexin A5. technetium Tc 99m HYNIC annexin V ... annexin A5. Known as: Calphobindin I, Annexin V, Lipocortin-V (More). A protein of the annexin family isolated from human ... Annexin A5 polymorphism (-1C--,T) and the presence of anti-annexin A5 antibodies in the antiphospholipid syndrome. ...
more infohttps://www.semanticscholar.org/topic/annexin-A5/115736

WikiGenes - ANXA3 - annexin A3WikiGenes - ANXA3 - annexin A3

The crystal structure of annexin A8 is similar to that of annexin A3. Réty, S., Sopková-de Oliveira Santos, J., Dreyfuss, L., ... The crystal structure of annexin A8 is similar to that of annexin A3 [17]. ... Annexin A3 is a potential angiogenic mediator. Park, J.E., Lee, d.o. .H., Lee, J.A., Park, S.G., Kim, N.S., Park, B.C., Cho, S ... The high-resolution crystal structure of human annexin III shows subtle differences with annexin V. Favier-Perron, B., Lewit- ...
more infohttps://www.wikigenes.org/e/gene/e/306.html

Anti-Annexin A3/ANXA3 antibody (ab228761) | AbcamAnti-Annexin A3/ANXA3 antibody (ab228761) | Abcam

Rabbit polyclonal Annexin A3/ANXA3 antibody. Validated in WB and tested in Human. Immunogen corresponding to recombinant ... Anti-Annexin A3/ANXA3 antibody. See all Annexin A3/ANXA3 primary antibodies. ... This product Rabbit Anti-Annexin A3/ANXA3 antibody (ab228761) WB Goat Anti-Rabbit IgG H&L (HRP) (ab205718) IHC-P, WB, ELISA, IP ... All lanes : Anti-Annexin A3/ANXA3 antibody (ab228761) at 1/1000 dilution. Lane 1 : A431 (human epidermoid carcinoma cell line) ...
more infohttps://www.abcam.com/annexin-a3anxa3-antibody-ab228761.html

Anxa3 (annexin A3) - Rat Genome DatabaseAnxa3 (annexin A3) - Rat Genome Database

... annexin A3) Alliance Pan paniscus (bonobo/pygmy chimpanzee) : ANXA3 (annexin A3) Canis lupus familiaris (dog) : ANXA3 (annexin ... ANXA3 (annexin A3). NCBI. Ortholog. Mus musculus (house mouse):. Anxa3 (annexin A3). Transitive Ortholog Pipeline. Transitive ... Anxa3 (annexin A3). Transitive Ortholog Pipeline. Transitive Ortholog Pipeline. Sus scrofa (pig):. ANXA3 (annexin A3). ... Homo sapiens (human) : ANXA3 (annexin A3) HGNC Alliance Mus musculus (house mouse) : Anxa3 (annexin A3) MGI Alliance Rattus ...
more infohttps://rgd.mcw.edu/rgdweb/report/gene/main.html?id=8764586

Annexin A3 Antibody (2F3) (NBP2-03373): Novus BiologicalsAnnexin A3 Antibody (2F3) (NBP2-03373): Novus Biologicals

Mouse Monoclonal Anti-Annexin A3 Antibody (2F3). Validated: WB, ICC/IF, IHC, IHC-P. Tested Reactivity: Human, Monkey. 100% ... Blogs on Annexin A3. There are no specific blogs for Annexin A3, but you can read our latest blog posts. ... Reviews for Annexin A3 Antibody (NBP2-03373) (0) There are no reviews for Annexin A3 Antibody (NBP2-03373). By submitting a ... Diseases for Annexin A3 Antibody (NBP2-03373). Discover more about diseases related to Annexin A3 Antibody (NBP2-03373). ...
more infohttps://www.novusbio.com/products/annexin-a3-antibody-2f3_nbp2-03373

anxa5 Protein, annexin A5 - Creative BioMartanxa5 Protein, annexin A5 - Creative BioMart

Annexin 5 is a;phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in; ... Annexin 5 has also been described as placental;anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin ... The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some;of which ... Recombinant Human Annexin A5, APC. E. coli. Human. N/A. +Inquiry. ANXA5-2624H. Recombinant Human Annexin A5, Biotin. E. coli. ...
more infohttps://www.creativebiomart.net/symbolsearch_ANXA5.htm

anxa3 Protein, annexin A3 - Creative BioMartanxa3 Protein, annexin A3 - Creative BioMart

This gene encodes a member of the annexin family.Members of this calcium-dependent phospholipid-binding protein family play a ... Recombinant Human Annexin A3, His-tagged. E. coli. Human. His. +Inquiry. ANXA3-1834H. Recombinant Human Annexin A3. E. coli. ... Annexin III; Annexin-3; AnnexinA3; AnnexinIII; ANX 3; ANX A3; ANX3; ANXA 3; ANXA3_HUMAN; Calcimedin 35 alpha; Inositol 1 2 ... Hamelin-Peyron, C; Vlaeminck-Guillem, V; et al. Prostate cancer biomarker annexin A3 detected in urines obtained following ...
more infohttps://www.creativebiomart.net/symbolsearch_ANXA3.htm

C-Reactive Protein and Annexin A5 Bind to Distinct Sites of Negatively Charged Phospholipids Present in Oxidized Low-Density...C-Reactive Protein and Annexin A5 Bind to Distinct Sites of Negatively Charged Phospholipids Present in Oxidized Low-Density...

Annexin A5 Binding to LDL. We observed a strong binding of annexin A5 to the captured oxidized LDL but not to captured native ... It is not known whether annexin A5 binds to LDL and/or interferes with binding of CRP to LDL. Association of annexin A5 with ... Moreover, we show that annexin A5 binds to a site at oxidized LDL different from the CRP binding site and that annexin A5 does ... Enzyme-Linked Immunosorbent Assay for CRP and Annexin A5 Binding to Antigens. Binding of CRP and annexin A5 to LDL was ...
more infohttp://atvb.ahajournals.org/content/25/4/717

In Vivo Monitoring of Parathyroid Hormone Treatment after Myocardial Infarction in Mice with [68Ga]Annexin A5 and [18F...In Vivo Monitoring of Parathyroid Hormone Treatment after Myocardial Infarction in Mice with [68Ga]Annexin A5 and [18F...

Annexin A5 positron emission tomography (PET) reveals the externalization of phosphatidylserine as a surrogate marker for ... 68Ga]Annexin A5 uptake was 7.4 ± 1.3 %ID/g within the infarction for the controls and 4.5 ± 1.9 %ID/g for the PTH group (p = . ... 68Ga]Annexin A5 PET can detect the effects of PTH treatment as a marker of apoptosis 2 days after MI; ex vivo examination ... Abstract[68Ga]Annexin A5 positron emission tomography (PET) reveals the externalization of phosphatidylserine as a surrogate ...
more infohttp://www.biomedsearch.com/nih/In-Vivo-Monitoring-Parathyroid-Hormone/25249170.html

Depletion of annexin A5, annexin A6 and collagen X causes no gross changes in matrix vesicle mediated mineralization | Murdoch...Depletion of annexin A5, annexin A6 and collagen X causes no gross changes in matrix vesicle mediated mineralization | Murdoch...

Depletion of annexin A5, annexin A6 and collagen X causes no gross changes in matrix vesicle mediated mineralization. Pubmed ID ... Depletion of annexin A5, annexin A6 and collagen X causes no gross changes in matrix vesicle mediated mineralization. Journal ...
more infohttps://www.mcri.edu.au/publications/depletion-annexin-a5-annexin-a6-and-collagen-x-causes-no-gross-changes-matrix-vesicle

Effect of Hydroxychloroquine on the Annexin A5 Resistance Assay in Antiphospholipid Antibody-Positive Patients with and without...Effect of Hydroxychloroquine on the Annexin A5 Resistance Assay in Antiphospholipid Antibody-Positive Patients with and without...

Effect of Hydroxychloroquine on the Annexin A5 Resistance Assay in Antiphospholipid Antibody-Positive Patients with and without ... on a blood test called the annexin A5 (AnxA5) resistance assay in people with antiphospholipid antibodies (aPL). People with ...
more infohttps://www.hss.edu/clinical-trials_antiphospholipid-syndrome-lupus-hydroxychloroquine.asp

Functional analysis of the human annexin A5 gene promoter: a downstream DNA element and an upstream long terminal repeat...Functional analysis of the human annexin A5 gene promoter: a downstream DNA element and an upstream long terminal repeat...

Human annexin A5 is a ubiquitous protein implicated in diverse signal transduction processes associated with cell growth and ... Promoter transcriptional activity was determined for a wide 5′ portion of the human annexin A5 gene, from bp −1275 to +79 ... Functional analysis of the human annexin A5 gene promoter: a downstream DNA element and an upstream long terminal repeat ... Functional analysis of the human annexin A5 gene promoter: a downstream DNA element and an upstream long terminal repeat ...
more infohttp://www.biochemj.org/content/356/2/571

Quantitative analysis of self-association and mobility of annexin a4 at the plasma membraneQuantitative analysis of self-association and mobility of annexin a4 at the plasma membrane

Annexin A4 (AnxA4) has been implicated in diverse cellular processes, including the regulation of exocytosis and ion-transport ... Quantitative analysis of self-association and mobility of annexin a4 at the plasma membrane. ... Annexins, found in most eukaryotic species, are cytosolic proteins that are able to bind negatively-charged phospholipids in a ...
more infohttps://repository.ubn.ru.nl/handle/2066/117526

99mTc-(CO)3 His-annexin A5 micro-SPECT demonstrates increased cell death by irinotecan during the vascular normalization window...99mTc-(CO)3 His-annexin A5 micro-SPECT demonstrates increased cell death by irinotecan during the vascular normalization window...

The apoptosis-detecting radiotracer Tc-99m-(CO)(3) His-annexin A5 was injected (18.5 MBq) in mice 12, 24, and 48 h after the ... The apoptosis-detecting radiotracer Tc-99m-(CO)(3) His-annexin A5 was injected (18.5 MBq) in mice 12, 24, and 48 h after the ... SPECT analyses demonstrated a significant increase in tumoral Tc-99m-(CO)(3) His-annexin A5 uptake 4 d after bevacizumab ... SPECT analyses demonstrated a significant increase in tumoral Tc-99m-(CO)(3) His-annexin A5 uptake 4 d after bevacizumab ...
more infohttps://biblio.ugent.be/publication/2141566

Annexin A5 Polyclonal Antibody | EpiGentekAnnexin A5 Polyclonal Antibody | EpiGentek

Description Annexin A5 Polyclonal Antibody. Unconjugated. Raised in Rabbit. Formulation Liquid. 0.03% Proclin 300,... ... Home » Antibodies » Primary Antibodies » Cell Structure & Function Antibodies » Apoptosis & Cell Death Antibodies » Annexin A5 ... Recombinant Cynops pyrrhogaster Annexin A5 protein (1-323AA). Storage. Shipped at 4°C. Upon delivery aliquot and store at -20°C ... Annexin A5 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.. Formulation. Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M ...
more infohttps://www.epigentek.com/catalog/annexin-a5-polyclonal-antibody-p-5439.html?currency=ww

Panorama Dashboard: /Panorama Public/2016/Barr-Gillespie - Annexin A5Panorama Dashboard: /Panorama Public/2016/Barr-Gillespie - Annexin A5

The phospholipid- and Ca2+-binding protein annexin A5 (ANXA5) is the most abundant membrane-associated protein of ~P23 mouse ... Annexin A5 is the Most-Abundant Membrane-Associated Protein in Stereocilia but is Dispensable ... GILL185_ANXA5_Ca2vsNoCa2_bundle_TGT2_DATA_Annexins_2016-03-18_16-08-41.sky.zip. (2 MB). 2016-03-23. 6. 27. 30. 167. 8. ... Annexins have been proposed to mediate Ca2+-dependent repair of membrane lesions, which could be part of the repair mechanism ...
more infohttps://panoramaweb.org/project/Panorama%20Public/2016/Barr-Gillespie%20-%20Annexin%20A5/begin.view

Annexin A5 is the Most Abundant Membrane-Associated Protein in Stereocilia but is Dispensable for Hair-Bundle Development and...Annexin A5 is the Most Abundant Membrane-Associated Protein in Stereocilia but is Dispensable for Hair-Bundle Development and...

Krey, J. F., Drummond, M., Foster, S., Porsov, E., Vijayakumar, S., Choi, D., ... Barr-Gillespie, P. (2016). Annexin A5 is the ... Annexin A5 is the Most Abundant Membrane-Associated Protein in Stereocilia but is Dispensable for Hair-Bundle Development and ... Annexin A5 is the Most Abundant Membrane-Associated Protein in Stereocilia but is Dispensable for Hair-Bundle Development and ... Annexin A5 is the Most Abundant Membrane-Associated Protein in Stereocilia but is Dispensable for Hair-Bundle Development and ...
more infohttps://ohsu.pure.elsevier.com/en/publications/annexin-a5-is-the-most-abundant-membrane-associated-protein-in-st

Gentaur Molecular :EIAab \ ELISA kit  35-beta calcimedin,Annexin A4,Annexin IV,Annexin-4,ANX4,ANXA4,Chromobindin-4,Endonexin I...Gentaur Molecular :EIAab \ ELISA kit 35-beta calcimedin,Annexin A4,Annexin IV,Annexin-4,ANX4,ANXA4,Chromobindin-4,Endonexin I...

Annexin A4,Annexin IV,Annexin-4,ANX4,ANXA4,Chromobindin-4,Endonexin I,Lipocortin IV,P32.5,PAP-II,Pig,Placental anticoagulant ... ELISA kit 35-beta calcimedin,Annexin A4,Annexin IV,Annexin-4,ANX4,ANXA4,Chromobindin-4,Endonexin I,Lipocortin IV,P32.5,PAP-II, ... ELISA kit 35-beta calcimedin,Annexin A4,Annexin IV,Annexin-4,ANX4,ANXA4,Chromobindin-4,Endonexin I,Lipocortin IV,P32.5,PAP-II, ... Product name : ELISA kit 35-beta calcimedin,Annexin A4,Annexin IV,Annexin-4,ANX4,ANXA4,Chromobindin-4,Endonexin I,Lipocortin IV ...
more infohttp://www.antibody-antibodies.com/product2767227-search-ELISA_kit__35_beta_calcimedin,Annexin_A4,Annexin_IV,Annexin_4,ANX4,ANXA4,Chromobindin_4,Endonexin_I,Lipocortin_IV,P32.5,PAP_II,Pig,Placental_anticoagulant_protein_II,PP4_X,Protein_II,Sus_scrofa.html

Annexin A5 polymorphism (-1C--|T) and the presence of anti-annexin A5 antibodies in the antiphospholipid syndrome.  - PubMed -...Annexin A5 polymorphism (-1C--|T) and the presence of anti-annexin A5 antibodies in the antiphospholipid syndrome. - PubMed -...

Annexin A5 polymorphism (-1C--,T) and the presence of anti-annexin A5 antibodies in the antiphospholipid syndrome.. de Laat B1 ... Annexin A5 (ANX‐A5) plasma concentration in measured plasma. ANX‐A5 levels were measured with "a sandwich‐ELISA‐type" assay. ... To investigate whether anti-annexin A5 immunoglobulin (Ig)M or IgG antibodies, or the -1C--,T polymorphism of annexin A5, is a ... Annexin A5 polymorphism (−1C→T) and the presence of anti‐annexin A5 antibodies in the antiphospholipid syndrome ...
more infohttps://phgkb.cdc.gov/PHGKB/phgHome.action?action=forward&dbsource=huge&id=20104

Annexin A5 haplotypes in familial hypercholesterolemia: lack of association with carotid intima-media thickness and...Annexin A5 haplotypes in familial hypercholesterolemia: lack of association with carotid intima-media thickness and...

Annexin A5 (ANXA5) has been suggested to possess antiatherogenic properties. We investigated whether ANXA5 genetic variations ... Annexin A5 haplotypes in familial hypercholesterolemia: lack of association with carotid intima-media thickness and ... Annexin A5 gene; Atherosclerosis; Cardiovascular disease; Carotid intima-media thickness; Familial hypercholesterolemia; ...
more infohttps://phgkb.cdc.gov/PHGKB/phgHome.action?action=forward&dbsource=huge&id=102258

Prolonged High-Fat Feeding Enhances Aortic 18F-FDG and 99mTc-Annexin A5 Uptake in Apolipoprotein E-Deficient and Wild-Type...Prolonged High-Fat Feeding Enhances Aortic 18F-FDG and 99mTc-Annexin A5 Uptake in Apolipoprotein E-Deficient and Wild-Type...

0.249 for 18F-FDG and 0.047 for 99mTc-annexin A5 at 25 wk). Conclusion: The accumulation of 18F-FDG and 99mTc-annexin A5 in ... At the ages of 10, 18, and 25 wk (5-15 mice per group at each time point), mice were injected with 18F-FDG or 99mTc-annexin A5 ... At 1 h after 18F-FDG injection (or 2 h after 99mTc-annexin A5 injection), mice were sacrificed, and the aortas were removed for ... Here, we evaluated the aortic uptake of 18F-FDG and 99mTc-annexin A5 in apolipoprotein E-deficient (apoE2/2) and wild-type mice ...
more infohttps://eprints.lib.hokudai.ac.jp/dspace/handle/2115/46755

A panel of tumor markers, calreticulin, annexin A2, and annexin A3 in upper tract urothelial carcinoma identified by proteomic...A panel of tumor markers, calreticulin, annexin A2, and annexin A3 in upper tract urothelial carcinoma identified by proteomic...

... annexin A2, and annexin A3. Calreticulin, annexin A2, and annexin A3 are very likely a panel of biomarkers with potential value ... annexin A2, annexin A3 and haptoglobin. The data of western blot and immunohistochemical analysis are consistent with the 2-DE ... annexin A2 and annexin A3 between the urine and tumor tissues of UTUC patients. CALR, annexin A2 and annexin A3 are very likely ... annexin A3 (spot 29) and Hp (spot 32 ~ 36). Side-by-side comparisons of CALR, ZAG, annexin A2, annexin A3 and Hp showed the ...
more infohttps://0-bmccancer-biomedcentral-com.brum.beds.ac.uk/articles/10.1186/1471-2407-14-363

Cardiovascular molecular imaging of apoptosis | SpringerLinkCardiovascular molecular imaging of apoptosis | SpringerLink

Annexin A5 has been more widely used to target PS exposure. Hiller and co-workers [54] recently reported on a new Anx A5-based ... This molecular imaging protocol is based upon the protein annexin A5 (Anx A5), which binds with high selectivity and with great ... Annexin A5. In the past decade, a molecular imaging protocol was established to detect and measure apoptosis in vitro and in ... The annexin A5 imaging protocol is emphasised since it is the most advanced protocol to measure apoptosis in both preclinical ...
more infohttps://link.springer.com/article/10.1007%2Fs00259-007-0443-0
  • Annexin A5 uptake in ischemic myocardium: demonstration of reversible phosphatidylserine externalization and feasibility of radionuclide imaging. (semanticscholar.org)
  • 68Ga]Annexin A5 uptake was 7.4 ± 1.3 %ID/g within the infarction for the controls and 4.5 ± 1.9 %ID/g for the PTH group (p = .013). (biomedsearch.com)
  • Here, we evaluated the aortic uptake of 18F-FDG and 99mTc-annexin A5 in apolipoprotein E-deficient (apoE2/2) and wild-type mice placed on high-fat diets. (hokudai.ac.jp)
  • Aortic tracer uptake [(ID%/g) · kg] remained stable with the regular diet in wild-type mice (0.054- 0.053 and 0.021-0.023 for 99mTc-annexin A5) but increased with cholesterol feeding in wild-type mice (0.164 for 18F-FDG and 0.036 for 99mTc-annexin A5 at 25 wk) and in apoE2/2 mice (0.249 for 18F-FDG and 0.047 for 99mTc-annexin A5 at 25 wk). (hokudai.ac.jp)
  • The annexin A5-mediated pathogenic mechanism in the antiphospholipid syndrome: role in pregnancy losses and thrombosis. (semanticscholar.org)
  • Annexin A5 is thought to have a role in the pathophysiology of the antiphospholipid syndrome (APS)-a syndrome characterised by recurrent thrombosis and pregnancy morbidity. (cdc.gov)
  • In flow cytometry, annexin V is commonly used to detect apoptotic cells by its ability to bind to phosphatidylserine, a marker of apoptosis when it is on the outer leaflet of the plasma membrane. (wikipedia.org)
  • Annexin A5 is used as a non-quantitative probe to detect cells that have expressed phosphatidylserine (PS) on the cell surface, an event found in apoptosis as well as other forms of cell death. (wikipedia.org)
  • The apoptosis-detecting radiotracer Tc-99m-(CO)(3) His-annexin A5 was injected (18.5 MBq) in mice 12, 24, and 48 h after the start of the irinotecan or NaCl treatment, and micro-SPECT was subsequently performed 3.5 h after injection of the radiotracer. (ugent.be)
  • The annexin A5 imaging protocol is emphasised since it is the most advanced protocol to measure apoptosis in both preclinical and clinical studies. (springer.com)
  • The annexin A5 affinity assay typically uses a conjugate of annexin V and a fluorescent or enzymatic label, biotin or other tags, or a radioelement, in a suitable buffer (annexin V binding to aminophospholipids is Ca2+ dependent). (wikipedia.org)
  • The assay combines annexin V staining of PS and PE membrane events with the staining of DNA in the cell nucleus with propidium iodide (PI) or 7-Aminoactinomycin D (AAD-7), distinguishing viable cells from apoptotic cells and necrotic cells. (wikipedia.org)
  • Methods and Results- We found that CRP and annexin A5 at physiological concentrations bind Ca ++ dependently to oxidized phosphatidylcholine present in oxidized LDL but not to native LDL. (ahajournals.org)
  • In Vivo Monitoring of Parathyroid Hormone Treatment after Myocardial Infarction in Mice with [68Ga]Annexin A5 and [18F]Fluorodeoxyglucose Positron Emission Tomography. (biomedsearch.com)
  • At the ages of 10, 18, and 25 wk (5-15 mice per group at each time point), mice were injected with 18F-FDG or 99mTc-annexin A5 after 12 h of fasting. (hokudai.ac.jp)
  • At 1 h after 18F-FDG injection (or 2 h after 99mTc-annexin A5 injection), mice were sacrificed, and the aortas were removed for welltype scintillation counting of radioactivity. (hokudai.ac.jp)
  • The discovery of a downstream element crucial for annexin A5 gene transcription, and its interaction with a potentially novel transcription factor or complex, may provide a clue to understanding the initiation of transcription by TATA-less, multiple start site promoters. (biochemj.org)
  • Annexin A5 haplotypes in familial hypercholesterolemia: lack of association with carotid intima-media thickness and cardiovascular disease risk. (cdc.gov)
  • Conclusion: The accumulation of 18F-FDG and 99mTc-annexin A5 in aortic tissues is influenced not only by the progression of atherosclerotic disease but also by cholesterol loading over time. (hokudai.ac.jp)
  • 16,17 In healthy, lean individuals, annexin A5 circulates at low concentrations in plasma (0.03 to 0.15 nmol/L, ie, 1 to 5 ng/mL). (ahajournals.org)
  • ANX‐A5 plasma concentrations are displayed as median (interquartile range). (cdc.gov)
  • Annexin A5 alone had no effect on binding of oxidized LDL to the cells. (ahajournals.org)
  • and (3) annexin A5 does not antagonize the CRP-induced enhanced binding of oxidized LDL to U937 cells. (ahajournals.org)
  • Annexins have been proposed to mediate Ca2+-dependent repair of membrane lesions, which could be part of the repair mechanism in hair cells after noise damage. (panoramaweb.org)
  • Increased plasma levels of annexin A5 were observed in patients with acute myocardial infarction or unstable angina, 18 and abundant expression is observed in atherosclerotic lesions of coronary arteries. (ahajournals.org)
  • T mutation in the annexin A5 gene (46/198 patients) was significantly associated with miscarriage (odds ratio 2.7, 95% confidence interval 1.1 to 6.7, independent risk factor). (cdc.gov)
  • Western blotting analysis and immunohistochemistry of tumor tissues and normal tissues from patients with UTUC were carried out to further verify five possible UTUC biomarkers, including zinc-alpha-2-glycoprotein, calreticulin, annexin A2, annexin A3 and haptoglobin. (beds.ac.uk)