A member of the annexin family that is a substrate for a tyrosine kinase, ONCOGENE PROTEIN PP60(V-SRC). Annexin A2 occurs as a 36-KDa monomer and in a 90-KDa complex containing two subunits of annexin A2 and two subunits of S100 FAMILY PROTEIN P11. The monomeric form of annexin A2 was formerly referred to as calpactin I heavy chain.
Protein of the annexin family exhibiting lipid interaction and steroid-inducibility.
A protein of the annexin family isolated from human PLACENTA and other tissues. It inhibits cytosolic PHOSPHOLIPASE A2, and displays anticoagulant activity.
Protein of the annexin family with a probable role in exocytotic and endocytotic membrane events.
Protein of the annexin family originally isolated from the electric organ of the electric ray Torpedo marmorata. It has been found in a wide range of mammalian tissue where it is localized to the apical membrane of polarized EPITHELIAL CELLS.
An annexin family member that plays a role in MEMBRANE FUSION and signaling via VOLTAGE-DEPENDENT CALCIUM CHANNELS.
A protein of the annexin family that catalyzes the conversion of 1-D-inositol 1,2-cyclic phosphate and water to 1-D-myo-inositol 1-phosphate.
Family of calcium- and phospholipid-binding proteins which are structurally related and exhibit immunological cross-reactivity. Each member contains four homologous 70-kDa repeats. The annexins are differentially distributed in vertebrate tissues (and lower eukaryotes) and appear to be involved in MEMBRANE FUSION and SIGNAL TRANSDUCTION.
A family of highly acidic calcium-binding proteins found in large concentration in the brain and believed to be glial in origin. They are also found in other organs in the body. They have in common the EF-hand motif (EF HAND MOTIFS) found on a number of calcium binding proteins. The name of this family derives from the property of being soluble in a 100% saturated ammonium sulfate solution.
Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a serine moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and serine and 2 moles of fatty acids.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Quaternary ammonium analog of ethidium; an intercalating dye with a specific affinity to certain forms of DNA and, used as diiodide, to separate them in density gradients; also forms fluorescent complexes with cholinesterase which it inhibits.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.
Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
A cell line derived from cultured tumor cells.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Extracellular vesicles generated by the shedding of CELL MEMBRANE blebs.
A family of G-protein-coupled receptors that was originally identified by its ability to bind N-formyl peptides such as N-FORMYLMETHIONINE LEUCYL-PHENYLALANINE. Since N-formyl peptides are found in MITOCHONDRIA and BACTERIA, this class of receptors is believed to play a role in mediating cellular responses to cellular damage and bacterial invasion. However, non-formylated peptide ligands have also been found for this receptor class.
Proteins prepared by recombinant DNA technology.
A short pro-domain caspase that plays an effector role in APOPTOSIS. It is activated by INITIATOR CASPASES such as CASPASE 9. Isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Organic compounds that contain technetium as an integral part of the molecule. These compounds are often used as radionuclide imaging agents.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Established cell cultures that have the potential to propagate indefinitely.
A family of intracellular CYSTEINE ENDOPEPTIDASES that play a role in regulating INFLAMMATION and APOPTOSIS. They specifically cleave peptides at a CYSTEINE amino acid that follows an ASPARTIC ACID residue. Caspases are activated by proteolytic cleavage of a precursor form to yield large and small subunits that form the enzyme. Since the cleavage site within precursors matches the specificity of caspases, sequential activation of precursors by activated caspases can occur.
Fluorescent probe capable of being conjugated to tissue and proteins. It is used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.
An in situ method for detecting areas of DNA which are nicked during APOPTOSIS. Terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 prime OH ends of either single- or double-stranded DNA. The terminal deoxynucleotidyl transferase nick end labeling, or TUNEL, assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA FRAGMENTATION.
A product of the lysis of plasminogen (profibrinolysin) by PLASMINOGEN activators. It is composed of two polypeptide chains, light (B) and heavy (A), with a molecular weight of 75,000. It is the major proteolytic enzyme involved in blood clot retraction or the lysis of fibrin and quickly inactivated by antiplasmins.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Experimentally induced chronic injuries to the parenchymal cells in the liver to achieve a model for LIVER CIRRHOSIS.
The genetic complement of an organism, including all of its GENES, as represented in its DNA, or in some cases, its RNA.
Databases devoted to knowledge about specific genes and gene products.
The physiological renewal, repair, or replacement of tissue.
Liver disease in which the normal microcirculation, the gross vascular anatomy, and the hepatic architecture have been variably destroyed and altered with fibrous septa surrounding regenerated or regenerating parenchymal nodules.
Genetic loci associated with a QUANTITATIVE TRAIT.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
The profession of writing. Also the identity of the writer as the creator of a literary production.
Collections of facts, assumptions, beliefs, and heuristics that are used in combination with databases to achieve desired results, such as a diagnosis, an interpretation, or a solution to a problem (From McGraw Hill Dictionary of Scientific and Technical Terms, 6th ed).
The guidelines and policy statements set forth by the editor(s) or editorial board of a publication.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
Antibodies produced by a single clone of cells.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
A plasma protein that circulates in increased amounts during inflammation and after tissue damage.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
A type of FLUORESCENCE SPECTROSCOPY using two FLUORESCENT DYES with overlapping emission and absorption spectra, which is used to indicate proximity of labeled molecules. This technique is useful for studying interactions of molecules and PROTEIN FOLDING.

Binding of annexin V to plasma membranes of human spermatozoa: a rapid assay for detection of membrane changes after cryostorage. (1/1343)

When the cell membrane is disturbed, phospholipid phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane. This is one of the earliest signs of apoptosis and can be monitored by the calcium-dependent binding of annexin V. Therefore, annexin V-binding, in conjunction with flow cytometry, was used to evaluate the integrity of the sperm plasma membrane after different cryostorage protocols: i.e. 10% (v/v) glycerol; sperm maintenance medium (MM); freezing medium TEST yolk buffer (TYB); or cryostorage without protection (cryoshock). Using a combination of two fluorescent dyes, annexin V and propidium iodide (PI), led to three groups of spermatozoa being identified: (i) viable spermatozoa (annexin V-negative and PI-negative); (ii) dead spermatozoa (annexin V-positive and PI-positive); and (iii) cells with impaired but integer plasma membrane (annexin V-positive and PI-negative). The percentage of vital annexin V-negative spermatozoa increased significantly (P < 0.05) from spermatozoa treated by cryoshock (15.0+/-1.2%) to spermatozoa cryopreserved by TYB (26.6+/-2.2%) via cryopreservation by 10% (v/v) glycerol (19.9+/-1.6%) and by MM (22.2 1.8%) and was associated with the percentage of motile spermatozoa (17.6+/-3.4% by glycerol; 19.6+/-3.7% by MM and 22.6+/-3.9% by TYB; P = 0.0001). Of the spermatozoa, 12-22% were annexin V-positive even though they did not bind to PI, indicating viability before as well as after cryostorage. The percentage of vital annexin V-positive spermatozoa was significantly correlated with different sperm motility parameters (velocity straight linear, r = 0.601, P = 0.018; percentage of linearly motile spermatozoa: r = 0.549, P = 0.034). We, therefore, concluded that annexin V-binding is more sensitive in detecting a deterioration of membrane functions than PI staining, and that a considerable percentage of spermatozoa might have dysfunctional plasma membranes besides dead or moribund cells. Of the cryopreservation protocols tested, TYB yielded the most viable spermatozoa. Therefore, we advocate the use of the annexin V-binding assay for the evaluation of the quality and integrity of spermatozoa.  (+info)

Overexpression of RelA causes G1 arrest and apoptosis in a pro-B cell line. (2/1343)

NF-kappaB/Rel family proteins form a network of post-translationally regulated transcription factors that respond to a variety of extracellular stimuli and mediate distinct cellular responses. These responses include cytokine gene expression, regulated cell cycle activation, and both the protection from and induction of the cell death program. To examine the function of individual Rel family proteins in B cell development and resolve their role in the signaling of apoptosis, we used a tetracycline-regulated gene expression system to overexpress either c-Rel or RelA in the transformed pro-B cell line 220-8. Elevated levels of RelA, but not c-Rel, induced a G1 cell cycle arrest followed by apoptosis. Both the DNA binding and transactivation domains of RelA were required for this effect. When RelA was overexpressed in the immature B cell line WEHI 231 or the mature B cell line M12, neither cell cycle arrest nor apoptosis was evident. The differential effects of elevated RelA levels in these cell lines suggests that susceptibility to NF-kappaB-induced apoptosis may reflect a relevant selection event during B cell development.  (+info)

Sodium salicylate activates caspases and induces apoptosis of myeloid leukemia cell lines. (3/1343)

Nonsteroidal antiinflammatory agents (NSAIA) have been shown to exert potent chemopreventive activity against colon, lung, and breast cancers. In this study, we show that at pharmacological concentrations (1 to 3 mmol/L) sodium salicylate (Na-Sal) can potently induce programmed cell death in several human myeloid leukemia cell lines, including TF-1, U937, CMK-1, HL-60, and Mo7e. TF-1 cells undergo rapid apoptosis on treatment with Na-Sal, as indicated by increased annexin V binding capacity, cpp-32 (caspase-3) activation, and cleavage of poly (ADP-ribose) polymerase (PARP) and gelsolin. In addition, the expression of MCL-1, an antiapoptotic member of the BCL-2 family, is downregulated during Na-Sal-induced cell death, whereas the expression of BCL-2, BAX, and BCL-XL is unchanged. Z-VAD, a potent caspase inhibitor, prevents the cleavage of PARP and gelsolin and rescues cells from Na-Sal-induced apoptosis. In addition, we show that Na-Sal accelerates growth factor withdrawal-induced apoptosis and synergizes with daunorubicin to induce apoptosis in TF-1 cells. Thus, our data provide a potential mechanism for the chemopreventive activity of NSAIA and suggest that salicylates may have therapeutic potential for the treatment of human leukemia.  (+info)

Temporal and spatial aspects of fragmentation in early human embryos: possible effects on developmental competence and association with the differential elimination of regulatory proteins from polarized domains. (4/1343)

This study examined the relationship between blastomere fragmentation in cultured human embryos obtained by in-vitro fertilization and the effect of fragmentation on the distribution of the following eight regulatory proteins found to be: (i) localized in the mature oocyte in subplasmalemmal, polarized domains; and (ii) unequally inherited by the blastomeres during cleavage: leptin, signal transducer and activator of transcription 3 (STAT3), Bax, Bcl-x, transforming growth factor beta 2 (TGF beta 2), vascular endothelial growth factor (VEGF), c-kit and epidermal growth factor R (EGF-R). Four basic patterns of fragmentation were observed. The severity of the impact of each type of fragmentation on the affected blastomere(s) and the developmental competence of the embryo appeared to be a function of the unique temporal and spatial features associated with the particular fragmentation pattern(s) involved in each instance. The findings demonstrate that certain patterns of fragmentation can result in the partial or near total loss of the eight regulatory proteins from specific blastomeres and that the developmental potential of the affected embryo can be particularly compromised if it occurs during the 1- or 2-cell stages. In contrast, fragmentation from portions of a fertilized egg or a blastomere(s) in a 2-cell embryo that do not contain the protein domains, or the complete loss by fragmentation of a regulatory protein domain-containing blastomere after the 4-cell stage does not necessarily preclude continued development to the blastocyst, although the normality and developmental potential of the embryo may be compromised. The possible association between fragmentation and apoptosis was examined by annexin V staining of plasma membrane phosphatidylserine and TUNEL analysis of blastomere DNA. No direct correlation between fragmentation and apoptosis was found following the analyses of fragmented embryos with these two markers. However, while we suggest that changes in cell physiology unrelated to apoptosis are the more likely causes of fragmentation, we cannot exclude the possibility that fragmentation itself may be an initiator of apoptosis if critical ratios or levels of developmentally important proteins are altered by partial or complete elimination of their polarized domains. The findings are discussed with respect to the possible developmental significance of regulatory protein polarization in human oocytes and preimplantation stage embryos.  (+info)

Interaction of heparin with annexin V. (5/1343)

The energetics and kinetics of the interaction of heparin with the Ca2+ and phospholipid binding protein annexin V, was examined and the minimum oligosaccharide sequence within heparin that binds annexin V was identified. Affinity chromatography studies confirmed the Ca2+ dependence of this binding interaction. Analysis of the data obtained from surface plasmon resonance afforded a Kd of approximately 21 nM for the interaction of annexin V with end-chain immobilized heparin and a Kd of approximately 49 nM for the interaction with end-chain immobilized heparan sulfate. Isothermal titration calorimetry showed the minimum annexin V binding oligosaccharide sequence within heparin corresponds to an octasaccharide sequence. The Kd of a heparin octasaccharide binding to annexin V was approximately 1 microM with a binding stoichiometry of 1:1.  (+info)

Oxidant-induced apoptosis in cultured human retinal pigment epithelial cells. (6/1343)

PURPOSE: To determine the mechanism of oxidant-induced cell death in cultured human retinal pigment epithelium (hRPE). METHODS: Cultured hRPE cells were treated with different concentrations of a chemical oxidant, t-butylhydroperoxide (tBH), for different periods of time. Apoptosis was determined with terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) and flow cytometry. Mitochondrial membrane potential (mtdelta psi) was measured by rhodamine 123 staining and subsequent flow cytometry. Release of mitochondrial cytochrome c (cyt c) and cleavage of procaspase 3 and caspase substrates were determined by western blot analysis. RESULTS: t-Butylhydroperoxide caused time- and dose-dependent activation of apoptosis in hRPE, indicated by characteristic morphologic changes; TUNEL-positive labeling; phosphatidylserine (PS) exposure; and procaspase 3, poly(ADP-ribose)polymerase, lamin, and tubulin cleavage. An early decrease of mtdelta psi was observed before caspase activation, together with the release of mitochondrial cyt c. CONCLUSIONS: Results indicate that tBH can induce apoptosis in hRPE, probably by triggering the mitochondrial permeability transition, which results in swelling and release of mitochondrial intermembrane proteins.  (+info)

Functional and structural properties of urinary tissue factor. (7/1343)

BACKGROUND: We have previously explored the clinical significance of urinary tissue factor (uTF) in patients with glomerulonephritis (GN) and malignancy. However, the functional and structural properties and putative cell of origin of uTF are poorly documented. In these studies we investigate these aspects of uTF. METHODS: Functional and structural properties of uTF were investigated using a one stage kinetic chromogenic assay, an enzyme-linked immunoabsorbent assay (ELISA) and transmission electron microscopy (TEM) on urine samples collected from healthy controls (n=69). The distribution of uTF and anionic phospholipid in the kidney were studied in sections from normal areas of nephrectomy specimens, using an immunoperoxidase technique. These were stained for tissue factor (TF) antigen and recombinant Annexin V. RESULTS: We found uTF to be present on subcellular particles as visualized by TEM. These particles contained anionic phospholipid as evidenced by binding to Annexin V fluorescein isothiocyanate (FITC). Although TF is present in urine in a functional and antigenic form no association was observed between the two. Using immunoperoxidase-based techniques, the cytoplasm of both distal and proximal tubules (but not glomerular cells) was positive for TF antigen and Annexin V. CONCLUSION: uTF is found on subcellular particles which provide lipid for its functional activity. Both uTF and its associated vesicles are found in the renal tubular cells.  (+info)

Localization and quantitation of cardiac annexins II, V, and VI in hypertensive guinea pigs. (8/1343)

Annexins are characterized by Ca2+-dependent binding to phospholipids. Annexin II mainly participates in cell-cell adhesion and signal transduction, whereas annexins V and VI also seem to regulate intracellular calcium cycling. Their abundance and localization were determined in left ventricle (LV) and right ventricle (RV) from hypertensive guinea pigs, during the transition from compensatory hypertrophy to heart failure. Immunoblot analysis of annexins II, V, and VI revealed an increased accumulation (2.6-, 1.45-, and 2.3-fold, respectively) in LV from hypertensive guinea pigs and no modification in RV. Immunofluorescent labeling of annexins II, V, and VI; of Na+-K+-ATPase; and of sarcomeric alpha-actinin showed that in control LV and RV, 1) annexin II is present in nonmuscle cells; 2) annexins V and VI are mainly observed in the sarcolemma and intercalated disks of myocytes; 3) annexins II, V, and VI strongly label endothelial cells and adventitia of coronary arteries; and 4) annexin VI is present in the media. At the onset of heart failure, the most striking changes are the increased protein accumulation in LV and the very strong labeling of annexins II, V, and VI in interstitial tissue, suggesting a role in fibrosis development and cardiac remodeling.  (+info)

IgG was the most frequently detected isotype in all four analyzed aPL subtypes. According to diagnostic criteria, aCL were more frequently found in patients with pAPS and sAPS than in those with SLE. Similarly, aPT were more often elevated in patients with pAPS (7/10) and sAPS (6/19) than in SLE (16/63; P , 0.05). The prevalence of anti-β2-GPI and aANXV antibodies did not differ between patient groups. Regardless of diagnosis, aPT were always detected in combination with aCL and/or anti-β2-GPI. aPT and aANXV were regularly present in the sera positive also for aCL and/or anti-β2-GPI. aANXV was detected as a single antibody in only one patient. In the pAPS group concurrent multiple aPL varieties (three or four) were significantly more frequently present than one or two types (8/10 versus 2/10; P , 0.05). Statistically significant associations of particular aPL with clinical symptoms were as follows: elevated IgG anti-β2-GPI with arterial thromboses, thrombocytopenia and foetal loss; IgG aPT ...
Annexin V Binding Buffer - Annexin V Binding Buffer has been formulated for flow cytometric labeling of apoptotic cells with Annexin V reagents.  The buffer is provided as a ready-to-use solution..
18F-FDG, a marker of the enhanced metabolism characteristic of activated inflammatory cells, and 99mTc-annexin A5, a marker of apoptosis, are both widely believed to be useful for the imaging of unstable atheroma (rupture-prone vulnerable plaques [VP]). Serum cholesterol functions as a proinflammatory factor, driving the formation of VP, and affects the immune responses of aortic tissues systemically. It is therefore reasonable to postulate that prolonged cholesterol loading may alter the aortic uptake of these tracers. Here, we evaluated the aortic uptake of 18F-FDG and 99mTc-annexin A5 in apolipoprotein E-deficient (apoE2/2) and wild-type mice placed on high-fat diets. Methods: Male apoE2/2 and wild-type (C57BL/6J) mice were maintained on high-fat diets after the age of 5 wk. Wild-type mice fed regular chow were used as controls. At the ages of 10, 18, and 25 wk (5-15 mice per group at each time point), mice were injected with 18F-FDG or 99mTc-annexin A5 after 12 h of fasting. At 1 h after ...
ELISA method for the measurement of IgM isotype auto antibodies targeted to annexin V. Assay designed with highly purified human recombinant annexin V, obtained with non-denaturing methods, for auto antibody immunocapture. These are then revealed with a goat antibody specific for IgM, micro fragments, and labeled with peroxidase.
Early in the apoptosis process, phosphatidylserine (PS) becomes exposed on the cell surface by flipping from the inner to outer leaflet of the cytoplasmic membrane. This event is thought to be important for macrophage recognition of cells undergoing apoptosis, thus allowing the cells to be rapidly phagocytosed. The binding of Annexin V to phosphatidylserine is calcium-dependent, reversible, but very tight, with a Kd of approximately 5 x 10-10 M. At low PS concentration, a binding ratio of 8 annexins to one PS has been reported. These features make Annexin V conjugates ideal for identifying membrane changes associated with apoptosis, by using either flow cytometric or in situ labeling methods. TACS® Annexin V kits are supplied with an Annexin V conjugate, an optimized binding buffer and propidium iodide. Cells are harvested and washed, then incubated with an Annexin V conjugate in binding buffer, for 15 minutes at room temperature prior to direct analysis. Alternatively, a secondary streptavidin ...
1HVD: Structural and electrophysiological analysis of annexin V mutants. Mutagenesis of human annexin V, an in vitro voltage-gated calcium channel, provides information about the structural features of the ion pathway, the voltage sensor and the ion selectivity filter
Figure 7. The PPARγ antagonist promoted E2-induced cell death in LTED breast cancer cell lines. A, DNA growth assay in MCF-7:5C cells. Cells were treated with E2 (1 nmol/L), T0070907 (5 μmol/L), or a combination of them for 7 days. Cells were harvested for DNA proliferation assay. **, P , 0.001. B, Apoptosis after T0070907 treatment in MCF-7:5C cells. Cells were treated with E2 (1 nmol/L), T0070907 (5 μmol/L), or a combination of them for 72 hours. Cells were then harvested for annexin V-binding assay. **, P , 0.001. C and D, Alteration of apoptosis-associated pathways in MCF-7:5C cells. Cells were treated as described in B and harvested in TRIzol. (C) TNFα and (D) HMOX1 expression levels were quantitated by RT-PCR. *, P , 0.05 and **, P , 0.001. E, T0070907 regulated cleaved PARP in MCF-7:5C cells. Cells were treated with E2 (1 nmol/L), T0070907 (5 μmol/L), or a combination of them for 3 days. Cleaved-PARP was detected using Western blotting. F, Expression of PPARγ and ERα after T0070907 ...
Annexin V is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Annexin V is a monomer, binds ATRX and EIF5B, and interacts with hepatitis B virus (HBV ...
Invitrogen™ eBioscience™ Annexin V, Recombinant Protein Unlabeled; 300μg Invitrogen™ eBioscience™ Annexin V, Recombinant Protein...
|p|Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry
We examined sera and placenta tissue from 156 patients in reproductive age with unexplained repeated pregnancy loss. Control group of eighty-four fertile healthy women was included. ELISA methods were used for detecting a panel of sera of anti-phospholipid antibodies (aPLs). Immunolocalization of annexin V receptors in 143 trophoblast-placenta specimen of 156 patients was investigated by the immunofluorescence technique using Annexin V-FITC, Apoptosis and Annexin V-CY3 commercial kits ...
For those of you who were following the Caspase-3 thread and those of you who contacted me directly, heres the information regarding Caspase-3 and Annexin V that I received from Pharmingen. Any furthur questions, please contact Padma at Pharmingen. Padma was most helpful. Lora , -----Original Message----- , From: pkodukula at pharmingen.com [SMTP:pkodukula at pharmingen.com] , Sent: Monday, October 04, 1999 10:14 AM , To: Barsky, Lora , Subject: RE: caspase-3 , , , , Dear Lora, , , Thank you for your interest in PharMingens products. This is in reply to , your , question about the caspase 3 and the annexin V staining. We are releasing , a kit , for the BrDU and cytokine staining. But the annexin V is also known to , work , with the caspase 3. This is not something that we routinely quality , control in , our company but we have had input from customers who have used this , method. The , annexin V binding to phosphatidyl serine is dependent on the CA++ ion , concentration and so a Ca++ ...
Supplementary MaterialsFigure S1: Expression of ITGAV, effect on viability and proliferation. UM-UC-3 and RT-4 cells were measured using the Alexa Fluor 488 annexin V/Dead Cell LDN-214117 Apoptosis Kit (Invitrogen). In addition, UM-UC-3 luc2 and RT-4 cells were seeded into a 6-well plate and exposed to a concentration series of GLPG0187 (0C500 ng/ml). 48 h after incubation, cells were harvested and processed for annexin V/PI staining. The percentage of viable (AnnexinV?/PI?), LDN-214117 dead (PI+/AnnexinV?), and total apoptotic cells (AnnexinV+) are shown (G). Proliferation rate (mitochondrial activity as assessed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (optical density at 490 nm)) in the 2 2 v kd clones (respectively closed circles and triangles) and NT (open circles) UM-UC3luc2 (H) and RT-4 (I) cells. The effects of GLPG0187 treatment on proliferation rate of UM-UC-3luc2 (J) and RT-4 cells (K) after 24, 48 and 72 h of treatment was assessed with ...
Annexin V-FITC Apoptosis assay results: Flow cytometry profiles represent Annexin-V-FITC staining in X-axis and PI in Y-axis: (a) control, i.e., neither PSi nor
BioAssay record AID 618683 submitted by ChEMBL: Induction of apoptosis in human HepG2 cells assessed as non-viable cells at 6 uL after 18 hrs by annexinV propidium iodide staining based flow cytometric analysis (Rvb = 14.32%).
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Apoptosis is the physiological process of cell death. Early stage apoptotic processes can be measured using fluorochrome, labeled Annexin V protein.
When calculating K, all the components should be in the same form/units. Therefore, the concentration of the gas would have to be calculated using the Ideal Gas Law of PV=nRT (divide by V on both sides, moles (n) over V = concentration, therefore, conc=P/RT). Finally the Kc can be calculated: Kc=[products]/[reactants ...
Euroopan keskuspankki (EKP) on euroalueen keskuspankki. Euroalueen muodostavat ne 19 Euroopan unionin jäsenvaltiota, jotka ovat ottaneet euron käyttöön. EKP:n tärkein tehtävä on ylläpitää hintavakautta euroalueella ja sen kautta säilyttää euron ostovoima.
TY - JOUR. T1 - Predictive value of anti-annexin V autoantibodies in the follow-up of vaso-occlusive crisis associated with sickle cell disease. AU - Sater, Mai S. AU - Almawi, Wassim Y. PY - 2011. Y1 - 2011. M3 - Article. VL - 5. SP - 363. JO - Biomarkers in Medicine. JF - Biomarkers in Medicine. SN - 1752-0363. IS - 3. ER - ...
BioAssay record AID 541028 submitted by ChEMBL: Induction of apoptosis in bovine BL3 cells assessed as early apoptotic cells after 24 hrs by annexin V/propidium iodide staining-based FACS analysis.
세포 죽음의 평가에 대한 정확한 방법을 설명합니다. 프로토콜은 세포 라인과 동물 모델의 광범위한 범위에서 기본 세포 40 % 허위 - 긍정적인 이벤트까지 표시 기존 Annexin V / propidium 요오드화물 (PI) 프로토콜에 따라이...
Invitrogen™ eBioscience™ Annexin V Apoptosis Detection Kit PerCP-eFluor™ 710 200 tests Invitrogen™ eBioscience™ Annexin V...
During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca2+-dependent affinity for PS and therefore is used as a probe for detecting apoptosis., Annexin V-FITC Apoptosis Detection Reagent, GTX14082, Applications: FACS; Flow cytometry/FACS; CrossReactivity:
Annexin II antibody (annexin A2) for ICC/IF, IHC-P, IP, RipA, WB. Anti-Annexin II pAb (GTX32446) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
Annexin V FITC Conjugate from human placenta; Synonyms: Calphosbindin I,Lipocortin V,Annexin V,PAP-1; find Sigma-Aldrich-A9210 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich
This article describes a simple way to monitor apoptosis and necrosis simultaneously in real time. The method is an alternative to annexin v staining methods that uses bioluminescence detection of annexin V binding, coupled with fluorescence detection of DNA release. Using a plate reader that records both luminescence and fluorescence, the RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay monitors the kinetics of apoptosis and secondary necrosis by repeatedly recording luminescence and fluorescence from the same cell sample.
Order monoclonal and polyclonal Annexin A13 antibodies for many applications. Selected quality suppliers for anti-Annexin A13 antibodies.
Group A:. Cycle 1 (3 weeks, combination therapy):. Carboplatin (AUC=5) administered I.V on week 1, day 1. 1000 mg/m ²/d 5-FU administered as a 96-hour C.I. starting on week 1, day 1.. 400 mg/m ² cetuximab administered I.V on week 2, day 1. 250 mg/m ² cetuximab administered I.V on week 3, day 1.. Cycle 2 (3 weeks, combination therapy):. Carboplatin (AUC=5) administered I.V on week 1, day 1. 1000 mg/m ²/d 5-FU administered as a 96-hour C.I. starting on week 1, day 1. 250 mg/m ² cetuximab administered I.V on weeks 1- 3, day 1.. After 7 cycles, participants may then receive cetuximab monotherapy until progression of disease, unacceptable toxicity, or another withdrawal criterion is met.. Due to protocol amendment in September 2011, any newly enrolled participants were placed into cetuximab and carboplatin (C) arm only. After protocol amendment February 2014, any newly enrolled participants will be placed into Group D arm only. ...
2015. Ghelani H, Razmovski-Naumovski V, Kyada AV, Parekh HS, Li GQ, Nammi S. Myoglobin and low density lipoprotein glycation in cardiac complications of diabetes: Current perspectives and therapeutic options. Indian J Med Res. 2015. In Press. Jadeja R, Devkar RV, Nammi S. Hepatoprotective potential of herbal medicine. Evid-Based Compl Alt. [Editorial]. 2015;2015.. Kam A, Li KM, Razmovski-Naumovski V, Nammi S, Chan K, Grau GE, et al. Curcumin reduces tumour necrosis factor-enhanced annexin V-positive microparticle release in human vascular endothelial cells. J Pharm Pharm Sci. 2015;18(4):424-33.. Thounaojam MC, Nammi S, Jadeja R. Natural products for the treatment of obesity, metabolic syndrome, and type 2 diabetes 2014. Evid-Based Compl Alt. [Editorial]. 2015;2015. DOI: 10.1155/2015/392681.. Yang WC, Chang CLT, Li CR, Nammi S, Cho WCS. Complementary and alternative medicine for diabetes 2014. Evid-Based Compl Alt. [Editorial]. 2015;2015. DOI: 10.1155/2015/685248.. 2014. Li Y, Tran VH, Kota BP, ...
Abcam provides specific protocols for Anti-Annexin A10 antibody (ab2343) : Western blot protocols, Immunohistochemistry protocols
Annexin A1兔多克隆抗体(ab65844)可与小鼠, 大鼠, 人样本反应并经WB, IP, IHC, ICC实验严格验证。所有产品均提供质保服务,中国75%以上现货。
Annexin A2兔多克隆抗体(ab90643)可与人样本反应并经WB, IHC实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
Rabbit polyclonal Integrin alpha V antibody validated for WB and tested in Human. Referenced in 1 publication. Immunogen corresponding to synthetic peptide
ANXA4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 321 amino acids (1-321 a.a.) and having a molecular mass of 36 kDa.
The cells were then put through movement cytometric analysis of Annexin V that brands apoptotic cells on differentiation time 7. stage embryo of and in BAC transgenic Ecteinascidin-Analog-1 mouse. They are horizontal areas as indicated in the right-sided illustration. The areas organized in parallel for and so are sequential. Dark arrows indicate the backdrop signal, frequently noticed on the margin from the tissues areas when executing hybridization on areas. Scale club; 100 m. (D) E9.0 embryo stained with X-gal following the tamoxifen administration in the pregnant feminine at E7.5. Take note only the center was Goat Polyclonal to Rabbit IgG stained, recommending that implemented tamoxifen activity was optimum within a day to induce the recombination of on the eight-somite stage [17] which it takes four to six 6 h for advancement through the five- to six-somite stage towards the eight-somite stage (one somite is the same as two hours) [72], this result implies that drawback of ...
What medication is light yellow round has V on one side and 2530 and a score line on the other side The KGB Agent answer: Not Medical Advice: Pill imprint 2530 V has been identified as Clonazepam 0.5 mg. It is used in the treatment of anxiety, bipolar disorder and more.
Hochwertige Annexin V FITC Kits für Apoptose Detektion mit Flow Cytometry. ✅ Namhafte Top-Hersteller im Vergleich. ✅ Top-Support bei Fragen.
Caveolin-1 (Cav-1), a family of ubiquitously expressed oligomeric structural proteins in many mammalian cells, has been shown to be an effective regulator of tumorigenesis. Recent studies have indicated that Cav-1 can promote resistance to chemotherapy in a variety of tumors. However, the regulation of Cav-1 on chemoresistance in ovarian cancer is still unknown. In the present study, the mRNA and protein expression level was investigated by RT-PCR and western blot analysis, respectively, and the 50% inhibitory concentration (IC50) value was measured by MTT assay. The protein expression level of P-glycoprotein (P-gp), Notch-1, p-Akt and p-NF-κB p65 were detected using western blot analysis and the apoptotic ratio was determined using the Annexin V-FITC/PI detection kit. The results showed that the mRNA and protein expression levels of Cav-1 were significantly higher in SKOV3/DDP and A2780/DDP than in SKOV3 and A2780, respectively. Knockdown of Cav-1 significantly decreased the IC50 value in ...
Mouse Monoclonal Anti-Annexin A3 Antibody (2F3). Validated: WB, ICC/IF, IHC, IHC-P. Tested Reactivity: Human, Monkey. 100% Guaranteed.
Tinción con anexina V y propidio yoduro (PI) ofrece a los investigadores una manera de identificar distintos tipos de muerte celular:...
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1BCW: Mutational and crystallographic analyses of interfacial residues in annexin V suggest direct interactions with phospholipid membrane components.
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Cell health assays analyze key cell indicators of cell death, cytotoxicity, cell viability or cell proliferation. Assays often measure specific markers (e.g. Annexin V, caspases), membrane integrity (e.g. LDH assay) or metabolic activity (e.g. MTT assay).
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Annexin A5 forms a shield around negatively charged phospholipid molecules, thus reducing their availability for coagulation. ... Thus, anti-annexin A5 antibodies increase phospholipid-dependent coagulation steps. The lupus anticoagulant antibodies are ...
Annexin A5-stained EVs, etc.); or c) descriptions of conditions or cell of origin (podocyte EVs, hypoxic EVs, large oncosomes, ...
Integrin, beta 5 has been shown to interact with PTK2, Annexin A5 and PAK4. Elevated levels of ITGB5 have been found in ... "Alpha v beta 5 integrin-dependent programmed cell death triggered by a peptide mimic of annexin V". Mol. Cell. 11 (5): 1151-62 ...
... has been shown to interact with SHC2, Annexin A5 and SHC1. Cluster of differentiation VEGF ...
... which encodes annexin A5. It is also neighbored by PP12613 located on the positive strand. The gene on chromosome 4 encoding ...
Annexin A5 affinity assay, a test for cells undergoing apoptosis, often uses flow cytometry Cell cycle analysis Coulter counter ...
... annexin A3 MeSH D08.811.277.352.640.125 - 3',5'-cyclic-GMP phosphodiesterase MeSH D08.811.277.352.640.150 - 3',5'-cyclic- ... cytochromes a3 MeSH D08.244.187.249 - cytochromes b MeSH D08.244.187.500 - cytochromes b5 MeSH D08.244.187.600 - cytochromes b6 ...
Annexin A3 MeSH D12.776.157.125.050.080 - annexin a4 MeSH D12.776.157.125.050.100 - annexin a5 MeSH D12.776.157.125.050.110 - ... annexin a6 MeSH D12.776.157.125.050.120 - annexin a7 MeSH D12.776.157.125.412.249 - calmodulin MeSH D12.776.157.125.412.311 - ... annexin a1 MeSH D12.776.157.125.050.060 - annexin a2 MeSH D12.776.157.125.050.070 - ...
However some annexins (Annexin A1, Annexin A2, and Annexin A5) have also been found outside the cellular environment, for ... Annexin, type I InterPro: IPR002388 Annexin, type II InterPro: IPR002389 Annexin, type III InterPro: IPR002390 Annexin, type IV ... In addition to annexin A-II, annexin A-XI has also been shown to organize cell membrane properties. Annexin A-XI is believed to ... The 310 amino acid annexin core has four annexin repeats, each composed of 5 alpha-helices. The exception is annexin A-VI that ...
Annexin A5, a human cellular protein. *ATC code A05 Bile and liver therapy, a subgroup of the Anatomical Therapeutic Chemical ... A5/1, A5/2 and A5/3, ciphers used in cellular phones ... Gibson A-5 mandolin, a Gibson mandolin. *Tippmann A-5, a semi- ... ICON A5, an American amphibious aircraft. Military[edit]. Aircraft[edit]. *A-5, an export version of Nanchang Q-5, a Chinese- ... Subfamily A5, a Rhodopsin-like receptors subfamily. *Noradrenergic cell group A5, a noradrenergic cell group located in the ...
The concept for the therapy is based on the anti-inflammatory properties of Annexin A5, a body own protective protein that acts ... Annexin Pharmaceuticals is a Swedish privately held biotech company developing new therapeutic approaches for inflammatory ... The company is currently focusing on treatment of peripheral artery disease (PAD). Martz, Lauren (May 26, 2014). "Annexin: ... CS1 maint: discouraged parameter (link) http://www.solidinfo.se/foretag/annexin-pharmaceuticals-ab/personer www. ...
... is a protein that in humans is encoded by the ANXA3 gene. It is abnormally expressed in fetuses of both IVF and ICSI ... "Entrez Gene: ANXA3 annexin A3". Zhang Y, Zhang YL, Feng C, et al. (September 2008). "Comparative proteomic analysis of human ... 2005). "Annexin A3 is a potential angiogenic mediator". Biochem. Biophys. Res. Commun. 337 (4): 1283-7. doi:10.1016/j.bbrc. ... "The high-resolution crystal structure of human annexin III shows subtle differences with annexin V.". Biochemistry. 35 (6): ...
... is a protein that in humans is encoded by the ANXA4 gene. Annexin IV (ANX4) belongs to the annexin family of calcium ... "Entrez Gene: ANXA4 annexin A4". Human ANXA4 genome location and ANXA4 gene details page in the UCSC Genome Browser. Römisch J, ... Dreier R, Schmid KW, Gerke V, Riehemann K (Aug 1998). "Differential expression of annexins I, II and IV in human tissues: an ... Han EK, Tahir SK, Cherian SP, Collins N, Ng SC (Jul 2000). "Modulation of paclitaxel resistance by annexin IV in human cancer ...
... (or annexin V) is a cellular protein in the annexin group. In flow cytometry, annexin V is commonly used to detect ... Annexin A5 showed upregulation in papillary thyroid carcinoma. Annexin A5 is used as a non-quantitative probe to detect cells ... Annexin A5 forms a shield around negatively charged phospholipid molecules. The formation of an annexin A5 shield blocks the ... The annexin A5 affinity assay typically uses a conjugate of annexin V and a fluorescent or enzymatic label, biotin or other ...
... a sequence for the insulin gene Adenosine A3 receptor, a human gene Annexin A3, a human gene ATC code A03 Drugs for functional ... A3, A03 or A.III may refer to: A3 paper, a paper size defined by ISO 216 A3 regulatory sequence, ... a band known as A3 in the U.S. to avoid confusion with the country group Alabama A-3, a Yamaha musical instrument product A3 ... a British Isles plants community Gibberellin A3, a plant hormone HLA-A3, a Human MHC Serotype HLA-A Subfamily A3, a rhodopsin- ...
This clustering of annexin A5 on the membrane greatly increases the intensity of annexin A5 when labeled with a fluorescent or ... Labeling of annexin A5 with fluorescent or radioactive molecules makes it possible to detect binding of labeled annexin A5 to ... Internalization amplifies additionally the intensity of the annexin A5 stained cell. Annexin A5 has been used to successively ... annexin A5 assembles into a trimeric cluster. This trimer consists of three annexin A5 molecules that are bound to each other ...
... which may be reflected by increased expression of the membrane fusion proteins NAPA and Annexin A3.[20] ...
Annexin A2, CAF-1, CDC25C, CHTF18, Cyclin D1, Cyclin O, Cyclin-dependent kinase 4, Cyclin-dependent kinase inhibitor 1C, DNMT1 ... Chromatin remodeling factor Clamp loader Cohesin DNA ligase DNA methyltransferase DNA polymerases E2 SUMO-conjugating enzyme E3 ...
Takano T, Fiore S, Maddox JF, Brady HR, Petasis NA, Serhan CN (May 1997). "Aspirin-triggered 15-epi-lipoxin A4 (LXA4) and LXA4 ... Ernst S, Lange C, Wilbers A, Goebeler V, Gerke V, Rescher U (Jun 2004). "An annexin 1 N-terminal peptide activates leukocytes ... "The synthetic peptide Trp-Lys-Tyr-Met-Val-Met-NH2 specifically activates neutrophils through FPRL1/lipoxin A4 receptors and is ... is not affected by lipoxin A4". Scandinavian Journal of Immunology. 56 (5): 470-6. doi:10.1046/j.1365-3083.2002.01149.x. PMID ...
Annexin II, which is attracted to negatively charged phospholipids, binds to p11 at the Ca2+ binding site. In addition, Annexin ... "Induction of endogenous genes following infection of human endothelial cells with an E1(-) E4(+) adenovirus gene transfer ... Kwon M, MacLeod TJ, Zhang Y, Waisman DM (Jan 2005). "S100A10, annexin A2, and annexin a2 heterotetramer as candidate ... Complexed with the annexin II, p11 binds receptor and channel proteins and guides them to the cell surface, resulting in ...
Annexin *A1. *A2. *A5. *Fibulin *FBLN1. *FBLN2. *FBLN5. *Vitamin D-dependent calcium-binding protein/Calbindin ...
2012). "The S100A10 subunit of the annexin A2 heterotetramer facilitates L2-mediated human papillomavirus infection". PLOS ONE ... E1, E2, E4, E5, E6, and E7) open reading frames (ORF), two late (L1 and L2) ORFs, and a non-coding long control region (LCR). ... and annexin A2 leading to entry of the virions into basal epithelial cells through clathrin-mediated endocytosis and/or ...
... lipoxin A4 (LXA4). Because of its interaction with lipoxin A4, FPR2 is also commonly named the ALX/FPR2 or just ALX receptor. ... Ac2-26 and Ac9-25 of Annexin A1 (ANXA1 or lipocortin 1), which at high concentrations fully stimulate neutrophil functions but ... lipoxin A4 (LXA4), and thereafter for a related arachidonic acid metabolite, the Epi-lipoxin, aspirin-triggered lipoxin A4 (i.e ... "Lipoxin A4 stable analogs are potent mimetics that stimulate human monocytes and THP-1 cells via a G-protein-linked lipoxin A4 ...
018229 ANXA6 Annexin 6 ANXA7 Annexin 7 AP1B1 Coated vesicles CLTA Clathrin A (vesicles) CLTB Clathrin B (vesicles) CLTC MTX2 ... 021178 E3 ubiquitin-protein ligase. Modulates cyclin B levels and participates in the regulation of cell cycle progression ... 003334 Homo sapiens ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature UBA2 NM_005499 UBA3 NM_003968 UBA5 NM_024818 ...
2002). „Phagocyte activation by Trp-Lys-Tyr-Met-Val-Met, acting through FPRL1/LXA4R, is not affected by lipoxin A4.". Scand. J ... 2004). „An annexin 1 N-terminal peptide activates leukocytes by triggering different members of the formyl peptide receptor ... 2001). „The synthetic peptide Trp-Lys-Tyr-Met-Val-Met-NH2 specifically activates neutrophils through FPRL1/lipoxin A4 receptors ...
These products are now termed lipoxin A4 and B4, respectively. While initially found to have in vitro activity suggesting that ... annexin A1 (an inhibitor of formation of pro-inflammatory metabolites of polyunsaturated fatty acids), and the gaseous ...
He KL, Deora AB, Xiong H, Ling Q, Weksler BB, Niesvizky R, Hajjar KA (July 2008). "Endothelial cell annexin A2 regulates ... "Cancer related mutations in NRF2 impair its recognition by Keap1-Cul3 E3 ligase and promote malignancy". Proceedings of the ... "cIAP1 and cIAP2 facilitate cancer cell survival by functioning as E3 ligases that promote RIP1 ubiquitination". Molecular Cell ... "Unanchored K48-linked polyubiquitin synthesized by the E3-ubiquitin ligase TRIM6 stimulates the interferon-IKKε kinase-mediated ...
Takano T, Fiore S, Maddox JF, Brady HR, Petasis NA, Serhan CN (May 1997). "Aspirin-triggered 15-epi-lipoxin A4 (LXA4) and LXA4 ... Annexin A1 (also termed ANXA1 and lipocortin 1) and its N-terminal peptides (Ac2-26 and Ac9-25). At low concentrations, these ... FMLP and lipoxin A4. Isolated mouse Olfactory bulb neurons also respond to a range of other fpr agonists. These results suggest ... and functional characterization of a murine lipoxin A4 receptor homologue gene". Journal of Immunology. 169 (6): 3363-9. doi: ...
Chemical structures of major endogenous estrogens, including estrone (E1), estradiol (E2), estriol (E3), and estetrol (E4).[9] ... "Activation of the annexin A1 pathway underlies the protective effects exerted by estrogen in polymorphonuclear leukocytes" ... Note the hydroxyl (-OH) groups: estrone (E1) has one, estradiol (E2) has two, estriol (E3) has three, and estetrol (E4) has ... The three major naturally occurring forms of estrogen in females are estrone (E1), estradiol (E2), and estriol (E3). Another ...
Genome organization of human papillomavirus type 16, one of the subtypes known to cause cervical cancer (E1-E7 early genes, L1- ... and annexin A2[93] leading to entry of the virions into basal epithelial cells through clathrin-mediated endocytosis and/or ... E1, E2, E4, E5, E6, and E7) open-reading frames (ORF), two late (L1 and L2) ORFs, and a non-coding long control region (LCR).[ ... "The S100A10 subunit of the annexin A2 heterotetramer facilitates L2-mediated human papillomavirus infection". PLoS One. 7 (8 ...
In addition, the tumor cells may also express adenosine A1 and A3 receptors associated with Gαi proteins, promoting both the ... Babiychuk EB, Draeger A (June 2006). "Regulation of ecto-5'-nucleotidase activity via Ca2+-dependent, annexin 2-mediated ...
Annexin A3 is a protein that in humans is encoded by the ANXA3 gene. It is abnormally expressed in fetuses of both IVF and ICSI ... "Entrez Gene: ANXA3 annexin A3". Zhang Y, Zhang YL, Feng C, et al. (September 2008). "Comparative proteomic analysis of human ... 2005). "Annexin A3 is a potential angiogenic mediator". Biochem. Biophys. Res. Commun. 337 (4): 1283-7. doi:10.1016/j.bbrc. ... "The high-resolution crystal structure of human annexin III shows subtle differences with annexin V.". Biochemistry. 35 (6): ...
Annexin A4 is a protein that in humans is encoded by the ANXA4 gene. Annexin IV (ANX4) belongs to the annexin family of calcium ... "Entrez Gene: ANXA4 annexin A4". Human ANXA4 genome location and ANXA4 gene details page in the UCSC Genome Browser. Römisch J, ... Dreier R, Schmid KW, Gerke V, Riehemann K (Aug 1998). "Differential expression of annexins I, II and IV in human tissues: an ... Han EK, Tahir SK, Cherian SP, Collins N, Ng SC (Jul 2000). "Modulation of paclitaxel resistance by annexin IV in human cancer ...
Compare Anti-Annexin A5 Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, reviews ... Anti-Annexin A5 Antibody Products. Anti-Annexin A5 antibodies are available from several suppliers. In humans, this protein is ... Antibodies to the Annexin A5 antigen may be useful for studying apoptosis. Visit the supplier page for more detailed antibody ... Annexin V is an early apoptotic marker and is often used for detecting apoptosis in cells. Annexins are a family of calcium- ...
Rabbit polyclonal Annexin A3/ANXA3 antibody. Validated in WB and tested in Human. Immunogen corresponding to recombinant ... Anti-Annexin A3/ANXA3 antibody. See all Annexin A3/ANXA3 primary antibodies. ... This product Rabbit Anti-Annexin A3/ANXA3 antibody (ab228761) WB Goat Anti-Rabbit IgG H&L (HRP) (ab205718) IHC-P, WB, ELISA, IP ... All lanes : Anti-Annexin A3/ANXA3 antibody (ab228761) at 1/1000 dilution. Lane 1 : A431 (human epidermoid carcinoma cell line) ...
... annexin A3) Alliance Pan paniscus (bonobo/pygmy chimpanzee) : ANXA3 (annexin A3) Canis lupus familiaris (dog) : ANXA3 (annexin ... ANXA3 (annexin A3). NCBI. Ortholog. Mus musculus (house mouse):. Anxa3 (annexin A3). Transitive Ortholog Pipeline. Transitive ... Anxa3 (annexin A3). Transitive Ortholog Pipeline. Transitive Ortholog Pipeline. Sus scrofa (pig):. ANXA3 (annexin A3). ... Homo sapiens (human) : ANXA3 (annexin A3) HGNC Alliance Mus musculus (house mouse) : Anxa3 (annexin A3) MGI Alliance Rattus ...
Annexin A5, ENX2, Placental Anticoagulant Protein 4, Placental Anticoagulant Protein I, Vascular Anticoagulant-Alpha, ... Thromboplastin Inhibitor, Calphobindin I, Anchorin CII, Endonexin II, Lipocortin V, Annexin V, Annexin-5, VAC-Alpha, RPRGL3, ... ANXA5 is a member of the annexin family of calcium-dependent phospholipid binding proteins which are involved in membrane- ...
The crystal structure of annexin A8 is similar to that of annexin A3. Réty, S., Sopková-de Oliveira Santos, J., Dreyfuss, L., ... The crystal structure of annexin A8 is similar to that of annexin A3 [17]. ... Annexin A3 is a potential angiogenic mediator. Park, J.E., Lee, d.o. .H., Lee, J.A., Park, S.G., Kim, N.S., Park, B.C., Cho, S ... The high-resolution crystal structure of human annexin III shows subtle differences with annexin V. Favier-Perron, B., Lewit- ...
A protein of the annexin family isolated from human PLACENTA and other tissues. It inhibits cytosolic PHOSPHOLIPASE A2, and ... Annexin A5, human. diannexin, human. technetium 99m tricarbonyl His-annexin A5. technetium Tc 99m HYNIC annexin V ... annexin A5. Known as: Calphobindin I, Annexin V, Lipocortin-V (More). A protein of the annexin family isolated from human ... Annexin A5 polymorphism (-1C--,T) and the presence of anti-annexin A5 antibodies in the antiphospholipid syndrome. ...
Mouse Monoclonal Anti-Annexin A3 Antibody (2F3). Validated: WB, ICC/IF, IHC, IHC-P. Tested Reactivity: Human, Monkey. 100% ... Blogs on Annexin A3. There are no specific blogs for Annexin A3, but you can read our latest blog posts. ... Reviews for Annexin A3 Antibody (NBP2-03373) (0) There are no reviews for Annexin A3 Antibody (NBP2-03373). By submitting a ... Diseases for Annexin A3 Antibody (NBP2-03373). Discover more about diseases related to Annexin A3 Antibody (NBP2-03373). ...
ANX4, ANXA4, Annexin-IV, Lipocortin-4, Lipocortin-IV, Annexin-4, Annexin A4, Endonexin I, Chromobindin-4, Protein II, P32.5, ... ANXA4 is part of the annexin family of calcium-dependent phospholipid binding proteins. ANXA4 has 45%-59% identity with other ...
Annexin A5 Protein Recombinant is available 3 times from supplier abbex at Gentaur.com shop ... Annexin A5 Protein Recombinant. Annexin A5 Protein Recombinant is available 3 times from Abbex labs abx260555 , Annexin A5 ... Annexin A4 Protein Recombinant Suppplier: abbex. Price: 295.25 USD Annexin A3 Protein Recombinant Suppplier: abbex. Price: ... abx260555 , Annexin A5 Protein (Recombinant) size: 5 µg , 295.25 USD Catalog number abx260555 ...
"Annexin A3" by people in this website by year, and whether "Annexin A3" was a major or minor topic of these publications. ... "Annexin A3" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... Below are the most recent publications written about "Annexin A3" by people in Profiles. ... Below are MeSH descriptors whose meaning is more general than "Annexin A3". ...
Annexin A5 positron emission tomography (PET) reveals the externalization of phosphatidylserine as a surrogate marker for ... 68Ga]Annexin A5 uptake was 7.4 ± 1.3 %ID/g within the infarction for the controls and 4.5 ± 1.9 %ID/g for the PTH group (p = . ... 68Ga]Annexin A5 PET can detect the effects of PTH treatment as a marker of apoptosis 2 days after MI; ex vivo examination ... Abstract[68Ga]Annexin A5 positron emission tomography (PET) reveals the externalization of phosphatidylserine as a surrogate ...
Annexin A5 Binding to LDL. We observed a strong binding of annexin A5 to the captured oxidized LDL but not to captured native ... It is not known whether annexin A5 binds to LDL and/or interferes with binding of CRP to LDL. Association of annexin A5 with ... Moreover, we show that annexin A5 binds to a site at oxidized LDL different from the CRP binding site and that annexin A5 does ... Enzyme-Linked Immunosorbent Assay for CRP and Annexin A5 Binding to Antigens. Binding of CRP and annexin A5 to LDL was ...
Annexin A5 (ANXA5) has been linked with a variety of cellular functions including: endocytosis, Ca2+ transfer, anticoagulation ... Annexin A5, ANXA5, Ca2+ channel, membrane interaction, membrane binding, PS, Phosphatidylserine, channel formation, structural ... Stewart, David John Ossian (2019) Investigating the metamorphic properties of annexin A5 during membrane integration. PhD ... The annexin superfamily is comprised of a range of Ca2+ dependent phospholipid binding proteins. Each of these proteins are ...
Annexin 5 is a;phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in; ... Annexin 5 has also been described as placental;anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin ... The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some;of which ... Recombinant Human Annexin A5, APC. E. coli. Human. N/A. +Inquiry. ANXA5-2624H. Recombinant Human Annexin A5, Biotin. E. coli. ...
Depletion of annexin A5, annexin A6 and collagen X causes no gross changes in matrix vesicle mediated mineralization. Pubmed ID ... Depletion of annexin A5, annexin A6 and collagen X causes no gross changes in matrix vesicle mediated mineralization. Journal ...
Effect of Hydroxychloroquine on the Annexin A5 Resistance Assay in Antiphospholipid Antibody-Positive Patients with and without ... on a blood test called the annexin A5 (AnxA5) resistance assay in people with antiphospholipid antibodies (aPL). People with ...
This gene encodes a member of the annexin family.Members of this calcium-dependent phospholipid-binding protein family play a ... Recombinant Human Annexin A3, His-tagged. E. coli. Human. His. +Inquiry. ANXA3-1834H. Recombinant Human Annexin A3. E. coli. ... Annexin III; Annexin-3; AnnexinA3; AnnexinIII; ANX 3; ANX A3; ANX3; ANXA 3; ANXA3_HUMAN; Calcimedin 35 alpha; Inositol 1 2 ... Hamelin-Peyron, C; Vlaeminck-Guillem, V; et al. Prostate cancer biomarker annexin A3 detected in urines obtained following ...
Annexin A3 antibody LS-C172488 is an unconjugated mouse monoclonal antibody to human Annexin A3 (ANXA3). Validated for IHC and ... ANXA3 , 35-alpha calcimedin , Annexin A3 , ANX3 , Annexin-3 , Annexin III , Calcimedin 35-alpha , Lipocortin III , Annexin III ... Annexin A3 antibody LS-C172488 is an unconjugated mouse monoclonal antibody to human Annexin A3 (ANXA3). Validated for IHC and ... Monoclonal Mouse anti‑Human ANXA3 / Annexin A3 Antibody (clone OTI5G4, IHC, WB) LS‑C172488 ...
Human annexin A5 is a ubiquitous protein implicated in diverse signal transduction processes associated with cell growth and ... Promoter transcriptional activity was determined for a wide 5′ portion of the human annexin A5 gene, from bp −1275 to +79 ... Functional analysis of the human annexin A5 gene promoter: a downstream DNA element and an upstream long terminal repeat ... Functional analysis of the human annexin A5 gene promoter: a downstream DNA element and an upstream long terminal repeat ...
The apoptosis-detecting radiotracer Tc-99m-(CO)(3) His-annexin A5 was injected (18.5 MBq) in mice 12, 24, and 48 h after the ... The apoptosis-detecting radiotracer Tc-99m-(CO)(3) His-annexin A5 was injected (18.5 MBq) in mice 12, 24, and 48 h after the ... SPECT analyses demonstrated a significant increase in tumoral Tc-99m-(CO)(3) His-annexin A5 uptake 4 d after bevacizumab ... SPECT analyses demonstrated a significant increase in tumoral Tc-99m-(CO)(3) His-annexin A5 uptake 4 d after bevacizumab ...
Annexin A4 (AnxA4) has been implicated in diverse cellular processes, including the regulation of exocytosis and ion-transport ... Quantitative analysis of self-association and mobility of annexin a4 at the plasma membrane. ... Annexins, found in most eukaryotic species, are cytosolic proteins that are able to bind negatively-charged phospholipids in a ...
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Description Annexin A5 Polyclonal Antibody. Unconjugated. Raised in Rabbit. Formulation Liquid. 0.03% Proclin 300,... ... Home » Antibodies » Primary Antibodies » Cell Structure & Function Antibodies » Apoptosis & Cell Death Antibodies » Annexin A5 ... Recombinant Cynops pyrrhogaster Annexin A5 protein (1-323AA). Storage. Shipped at 4°C. Upon delivery aliquot and store at -20°C ... Annexin A5 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.. Formulation. Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M ...
Annexin A1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent ... The Annexin family is composed of at least thirteen mammalian genes ( ... Clone M017 was generated from a proprietary antigen related to human Annexin A5 in the MDA-MB-231 breast cancer cell line. *For ... Annexin A5 is a PKC inhibitor, directly interacts with VEGFR2 receptor, and binds phosphatidylserine to inhibit blood ...
Annexin A5 has been more widely used to target PS exposure. Hiller and co-workers [54] recently reported on a new Anx A5-based ... This molecular imaging protocol is based upon the protein annexin A5 (Anx A5), which binds with high selectivity and with great ... Annexin A5. In the past decade, a molecular imaging protocol was established to detect and measure apoptosis in vitro and in ... The annexin A5 imaging protocol is emphasised since it is the most advanced protocol to measure apoptosis in both preclinical ...
title = "Annexin A5 binds to lipopolysaccharide and reduces its endotoxin activity",. abstract = "Annexin A5 (AnxA5) has a high ... Annexin A5 binds to lipopolysaccharide and reduces its endotoxin activity. Jacob H. Rand, Xiao Xuan Wu, Elaine Y. Lin, ... Annexin A5 binds to lipopolysaccharide and reduces its endotoxin activity. / Rand, Jacob H.; Wu, Xiao Xuan; Lin, Elaine Y.; ... Rand, J. H., Wu, X. X., Lin, E. Y., Griffel, A., Gialanella, P., & McKitrick, J. C. (2012). Annexin A5 binds to ...
The phospholipid- and Ca2+-binding protein annexin A5 (ANXA5) is the most abundant membrane-associated protein of ~P23 mouse ... Annexin A5 is the Most-Abundant Membrane-Associated Protein in Stereocilia but is Dispensable ... GILL185_ANXA5_Ca2vsNoCa2_bundle_TGT2_DATA_Annexins_2016-03-18_16-08-41.sky.zip. (2 MB). 2016-03-23. 6. 27. 30. 167. 8. ... Annexins have been proposed to mediate Ca2+-dependent repair of membrane lesions, which could be part of the repair mechanism ...
... and phospholipid-binding protein that belongs to the annexin family, which is involved in the development of multiple tumour ... Annexin A4 (ANXA4) is a Ca2+- ... Annexin A4 (ANXA4) is a Ca2+- and phospholipid-binding protein ... annexin A4 calcium/calmodulin-dependent protein kinase II gamma carbonic anhydrase SUMO membrane-cytoplasm translocation ... Membrane-cytoplasm translocation of annexin A4 is involved in the metastasis of colorectal carcinoma. Aging (Albany NY). 2021; ...
  • Annexin A3 is a protein that in humans is encoded by the ANXA3 gene. (wikipedia.org)
  • The protein may also be known as Annexin V, ANX5, ENX2, HEL-S-7, PP4, CBP-I, and PAP-I. The reported amino acid length is 320 and the expected mass is 35.9 kDa. (biocompare.com)
  • A protein of the annexin family isolated from human PLACENTA and other tissues. (semanticscholar.org)
  • Description This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. (gentaur.com)
  • A protein of the annexin family that catalyzes the conversion of 1-D-inositol 1,2-cyclic phosphate and water to 1-D-myo-inositol 1-phosphate. (umassmed.edu)
  • Objective- To investigate binding of C-reactive protein (CRP) and annexin A5, 2 proteins with high affinity for negatively charged phospholipids, to oxidized low-density lipoprotein (LDL) and the consequences of these interactions for subsequent binding of oxidized LDL to monocyte/macrophage-like U937 cells. (ahajournals.org)
  • Annexin A5 is an intracellular protein that is abundantly present in endothelial cells and platelets, and which exhibits a high affinity for anionic phospholipids in membranes. (ahajournals.org)
  • The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some;of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. (creativebiomart.net)
  • Annexin 5 is a;phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in;cellular signal transduction, inflammation, growth and differentiation. (creativebiomart.net)
  • Annexin 5 has also been described as placental;anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin;CII. (creativebiomart.net)
  • This gene encodes a member of the annexin family.Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways.This protein functions in the inhibition of phopholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. (creativebiomart.net)
  • Human annexin A5 is a ubiquitous protein implicated in diverse signal transduction processes associated with cell growth and differentiation, and its gene regulation is an important component of this function. (biochemj.org)
  • Annexin A2 is a cytoskeletal calcium-dependent phospholipid binding protein, which has been shown to be a mediator of corticosteroid activity, a substrate for serine/threonine kinases and growth regulated tyrosine kinases, and may play a role in secretion. (ecmbio.com)
  • Clone M017 detects a 35 kDa* protein corresponding to the molecular mass of Annexin A5 on SDS-PAGE immunoblots of human A431, MDA-MB-231, MeWo, and PC3 cells, as well as human skin, breast, lung, and brain. (ecmbio.com)
  • In addition, mass spectrometry analysis of immunoprecipitates using AM0171 in human MDA-MB-231 cell lysates confirms this antibody only detects Annexin A5 protein. (ecmbio.com)
  • Annexin A4 (ANXA4) is a Ca 2+ - and phospholipid-binding protein that belongs to the annexin family, which is involved in the development of multiple tumour types via NF-κB signalling. (aging-us.com)
  • The phospholipid- and Ca2+-binding protein annexin A5 (ANXA5) is the most abundant membrane-associated protein of ~P23 mouse vestibular hair bundles, the inner ear's sensory organelle. (panoramaweb.org)
  • ANXA5 (Annexin A5) is a Protein Coding gene. (genecards.org)
  • Here we show that annexin-A5 (AnxA5), a protein that self-assembles into two-dimensional (2D) arrays on membranes upon Ca2+ activation, promotes membrane repair. (uea.ac.uk)
  • Annexin V is a Ca2+ dependent phospholipid-binding protein with high affinity for PS. (nih.gov)
  • Annexin A3 (ANXA3), is a crucial member of the membrane associated protein superfamily, whose function research is still insufficient. (bvsalud.org)
  • Annexin A5 (ANXA5), a Ca 2+ and phospholipid binding protein, interacts with the N-terminal leucine-rich repeats of polycystin-1 (TC# 1.A.5.1.2). (tcdb.org)
  • Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. (abcam.com)
  • Western Blot: Annexin V Antibody (3B9B7) [NBP2-52414] - Analysis using ANXA5 mAb against human ANXA5 (AA: 160-320) recombinant protein. (novusbio.com)
  • It harbours binding sites for cytoplasmic protein ligands that can be targeted to membranes through the annexin-core-mediated phospholipid interaction. (nature.com)
  • The primary interest of our laboratory is the investigation of the effects of antiphospholipid (aPL) antibodies on an anticoagulant protein, annexin A5 (previously known as annexin V). The antiphospholipid (aPL) syndrome is an enigmatic autoimmune disorder that is characterized by thrombosis and/or recurrent spontaneous pregnancy losses that occur in patients who have evidence for antibodies against phospholipid-binding proteins. (yu.edu)
  • Annexin A5 (A5) is a phospholipid-binding protein with potent anticoagulant properties that are a consequence of its forming 2-dimensional crystals over membranes containing anionic phospholipids. (yu.edu)
  • Anti-Annexin A5 antibodies are available from several suppliers. (biocompare.com)
  • Antibodies to the Annexin A5 antigen may be useful for studying apoptosis. (biocompare.com)
  • Your search returned 479 Annexin A5 Antibodies across 40 suppliers. (biocompare.com)
  • T) and the presence of anti-annexin A5 antibodies in the antiphospholipid syndrome. (semanticscholar.org)
  • This effect was independent of the presence of complement and could be inhibited by irrelevant IgG and by antibodies to CD64 but not by annexin A5. (ahajournals.org)
  • The purpose of this study is to determine the effects of a medication, hydroxychloroquine (HCQ), on a blood test called the annexin A5 (AnxA5) resistance assay in people with antiphospholipid antibodies (aPL). (hss.edu)
  • For the detection of anti-annexin A5 antibodies and the measurement of annexin A5 plasma levels, ELISA-type methods were used. (cdc.gov)
  • 71 patients were positive for annexin A5 IgM or IgG antibodies, of whom 53 patients were positive for anti-annexin A5 IgG antibodies and 27 of 198 patients were positive for anti-annexin A5 IgM antibodies. (cdc.gov)
  • The prevalence of IgM or IgG anti-annexin A5 antibodies was not significantly associated with thrombosis or miscarriage on multivariate analysis. (cdc.gov)
  • The detection of anti-annexin A5 antibodies does not seem relevant for estimating the risk for thrombosis or miscarriage in APS. (cdc.gov)
  • Plasma annexin A5, anti-annexin A5 antibodies and annexin A5 polymorphism in Egyptian female patients with systemic lupus erythematosus and antipho. (cdc.gov)
  • The present study aimed to find out a possible relationship between annexin A5 (gene polymorphism, antibodies or plasma level) and the pathophysiology of SLE, APS and RPL. (cdc.gov)
  • Anti-annexin A5 antibodies (IgG and IgM) and annexin A5 plasma level were measured by Enzyme Linked Immunosorbent Assay (ELISA). (cdc.gov)
  • We were the first to propose and demonstrate that aPL antibodies disrupt the annexin A5 anticoagulant shield on artificial bilayers and on cell membranes. (yu.edu)
  • Western Blot: Annexin A3 Antibody (2F3) [NBP2-03373] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Annexin A3 (Right lane) cDNA for 48 hrs and lysed. (novusbio.com)
  • Immunocytochemistry/ Immunofluorescence: Annexin A3 Antibody (2F3) [NBP2-03373] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Annexin A3. (novusbio.com)
  • Immunohistochemistry-Paraffin: Annexin A3 Antibody (2F3) [NBP2-03373] - Staining of paraffin-embedded Human prostate tissue using anti-Annexin A3 mouse monoclonal antibody. (novusbio.com)
  • Annexin A3 antibody LS-C172488 is an unconjugated mouse monoclonal antibody to human Annexin A3 (ANXA3). (lsbio.com)
  • The efficacy of antihuman VEGF antibody (bevacizumab) with or without irinotecan was evaluated in a colorectal cancer xenograft using Tc-99m-(CO)(3) His-annexin A5. (ugent.be)
  • The blot was probed with mouse monoclonal anti-Annexin A5 antibody (AM0171) at 1:500 (lanes 1-5). (ecmbio.com)
  • The asterisk shows the antibody heavy chain in immunopreciptates at 50 kDa, while Annexin A5 band is observed at 35 kDa. (ecmbio.com)
  • Analytical sensitivity of the anti‐annexin A5 antibody ELISA. (cdc.gov)
  • Anti-annexin A5 IgM was the antibody associated with RPL in this group of APS patients. (cdc.gov)
  • Western Blot: Annexin V Antibody (3B9B7) [NBP2-52414] - Analysis using ANXA5 mAb against HEK293 (1) and ANXA5 (AA: 160-320)-hIgGFc transfected HEK293 (2) cell lysate. (novusbio.com)
  • Immunocytochemistry/ Immunofluorescence: Annexin V Antibody (3B9B7) [NBP2-52414] - Analysis of Hela cells using ANXA5 mouse mAb (green). (novusbio.com)
  • Flow Cytometry: Annexin V Antibody (3B9B7) [NBP2-52414] - An intracellular stain was performed on HeLa cells with Annexin V Antibody (3B9B7) NBP2-52414AF488 and a matched isotype control (orange). (novusbio.com)
  • Western Blot: Annexin V Antibody (3B9B7) [NBP2-52414] - Analysis using ANXA5 mouse mAb against HepG2 (1), PNAC-1 (2), NIH/3T3 (3), Hela (4), MCF-7 (5), and A431 (6) cell lysate. (novusbio.com)
  • Paraffin-embedded mouse cornea is stained with Annexin V Antibody used at 1:200 dilution. (antibodies-online.com)
  • Antibody-mediated interference with annexins in the antiphospholipid syndrome. (yu.edu)
  • Detection of antibody-mediated reduction of annexin A5 anticoagulant activity in plasmas of patients with the antiphospholipid syndrome. (yu.edu)
  • By polynomial first‐order equation, an average R 2 of 0.9929 was calculated for the anti‐annexin A5 IgG ELISA, and 0.9926 for the anti‐annexin A5 IgM ELISA. (cdc.gov)
  • Anti-annexin A5 IgM level was significantly higher among APS patients and was associated with RPL (p = 0.005, odds ratio = 23.75, 95% confidence interval = 2.15 - 262.48). (cdc.gov)
  • Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. (wikipedia.org)
  • ANXA5 is a member of the annexin family of calcium-dependent phospholipid binding proteins which are involved in membrane-related activity along exocytotic and endocytotic pathways. (prospecbio.com)
  • ANXA4 is part of the annexin family of calcium-dependent phospholipid binding proteins. (prospecbio.com)
  • The annexin superfamily is comprised of a range of Ca2+ dependent phospholipid binding proteins. (gla.ac.uk)
  • Annexins, found in most eukaryotic species, are cytosolic proteins that are able to bind negatively-charged phospholipids in a calcium-dependent manner. (ru.nl)
  • Taken together, our results indicate that annexin A5 may play an important role in TMS-mediated mitochondrial apoptosis through the regulation of proapoptotic proteins and VDAC1 expression. (bvsalud.org)
  • Combined the experimental data in the urine and in tumor tissues collected from patients with UTUC, the crucial over-expressed proteins are calreticulin, annexin A2, and annexin A3. (beds.ac.uk)
  • Annexins are a multigene family of Ca 2+ -regulated proteins that are characterized by a unique Ca 2+ - and membrane-binding module - the annexin core domain. (nature.com)
  • A unique class of these proteins - annexins - can bind to certain membrane phospholipids in a Ca 2+ -dependent manner, providing a link between Ca 2+ signalling and membrane functions. (nature.com)
  • By forming networks on the membrane surface, annexins can function as organizers of membrane domains and membrane-recruitment platforms for proteins with which they interact. (nature.com)
  • Liemann, S. & Lewit-Bentley, A. Annexins: a novel family of calcium- and membrane-binding proteins in search of a function. (nature.com)
  • Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. (biolegend.com)
  • ANX‐A5 levels were measured with "a sandwich‐ELISA‐type" assay. (cdc.gov)
  • Abstract[68Ga]Annexin A5 positron emission tomography (PET) reveals the externalization of phosphatidylserine as a surrogate marker for apoptosis. (biomedsearch.com)
  • Conclusions: Molecular imaging, using radiolabeled annexin A5, allows the detection of acute resolution of apoptosis as a result of caspase inhibition in experimental atherosclerosis. (fujita-hu.ac.jp)
  • Reutelingsperger, Chris P.M. / Engineered annexin A5 variants have impaired cell entry for molecular imaging of apoptosis using pretargeting strategies . (utmb.edu)
  • Swairjo, M. A. & Seaton, B. A. Annexin structure and membrane interactions: a molecular perspective. (nature.com)
  • Flow cytometry analyses of inflammatory cells obtained from four naturally cysticercotic pigs, and stained with Annexin-V/PI, showed lymphocytes expressing phosphatidylserine with values of 0, 6, 41 and 56% on their outer surfaces. (biomedsearch.com)
  • (B) Cell viability was measured at different time points after stimulation of cell death using A5/PI staining and then analyzed by flow cytometry (mean ± SD). (nih.gov)
  • 19 Because of its high affinity for anionic phospholipids, similar to CRP, annexin A5 might have an inhibitory effect on macrophage uptake of oxidized LDL via scavenger receptors. (ahajournals.org)
  • This core domain enables Ca 2+ -bound annexins to peripherally dock onto membranes that contain negatively charged phospholipids. (nature.com)
  • This gene encodes a member of the annexin family. (wikipedia.org)
  • Annexin A5 uptake in ischemic myocardium: demonstration of reversible phosphatidylserine externalization and feasibility of radionuclide imaging. (semanticscholar.org)
  • Lactadherin binding and phosphatidylserine expression on cell surface-comparison with annexin A5. (semanticscholar.org)
  • Annexin A5 is a PKC inhibitor, directly interacts with VEGFR2 receptor, and binds phosphatidylserine to inhibit blood coagulation. (ecmbio.com)
  • Annexin A5 (AnxA5) has a high affinity for phosphatidylserine. (elsevier.com)
  • Phosphatidylserine (PS) on apoptotic cells is a target for diagnosis and therapy using annexin A5 (anxA5). (utmb.edu)
  • Annexin V-PE-Cy5 Apoptosis Staining / Detection Kit (ab14159) is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. (abcam.com)
  • Quantitative real-time PCR analysis and Western blot analysis showed that the expression of annexin A5 was strongly increased in TMS-treated cells . (bvsalud.org)
  • In comparison with existing traditional tests the Annexin V assay is sensitive and easy to perform. (nih.gov)
  • The Annexin V assay offers the possibility of detecting early phases of apoptosis before the loss of cell membrane integrity and permits measurements of the kinetics of apoptotic death in relation to the cell cycle. (nih.gov)
  • Detection of annexin A5 (-1C/T) gene polymorphism was done by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) assay. (cdc.gov)
  • 68Ga]Annexin A5 uptake was 7.4 ± 1.3 %ID/g within the infarction for the controls and 4.5 ± 1.9 %ID/g for the PTH group (p = .013). (biomedsearch.com)
  • Here, we evaluated the aortic uptake of 18F-FDG and 99mTc-annexin A5 in apolipoprotein E-deficient (apoE2/2) and wild-type mice placed on high-fat diets. (hokudai.ac.jp)
  • Aortic tracer uptake [(ID%/g) · kg] remained stable with the regular diet in wild-type mice (0.054- 0.053 and 0.021-0.023 for 99mTc-annexin A5) but increased with cholesterol feeding in wild-type mice (0.164 for 18F-FDG and 0.036 for 99mTc-annexin A5 at 25 wk) and in apoE2/2 mice (0.249 for 18F-FDG and 0.047 for 99mTc-annexin A5 at 25 wk). (hokudai.ac.jp)
  • Annexin V binding was assessed using bivariate FCM, and cell staining was evaluated with fluorescein isothiocyanate (FITC)-labelled Annexin V (green fluorescence), simultaneously with dye exclusion of propidium iodide (PI) (negative for red fluorescence). (nih.gov)
  • (C) Example single A5-FITC-positive necroptotic cells, imaged using live microscopy. (nih.gov)
  • (G-H) 3 x 10 4 HaCaT cells per well in 96-well plate were stimulated, and PI and A5-FITC were added. (nih.gov)
  • Clone M017 was generated from a proprietary antigen related to human Annexin A5 in the MDA-MB-231 breast cancer cell line. (ecmbio.com)
  • Annexin A5 alone had no effect on binding of oxidized LDL to the cells. (ahajournals.org)
  • and (3) annexin A5 does not antagonize the CRP-induced enhanced binding of oxidized LDL to U937 cells. (ahajournals.org)
  • Annexin A6 reverses transformation of A431 cells after overexpression, and this effect may involve annexin A6 targeting of p120 RasGAP to the plasma membrane to inactivate Ras. (ecmbio.com)
  • Annexins have been proposed to mediate Ca2+-dependent repair of membrane lesions, which could be part of the repair mechanism in hair cells after noise damage. (panoramaweb.org)
  • In the present study, the possible roles of annexin A5 in TMS-mediated apoptosis were investigated in MCF7 human breast cancer cells . (bvsalud.org)
  • When cells were treated with TMS, the levels of Bax, and Bak as well as annexin A5 were strongly enhanced. (bvsalud.org)
  • Detection of A5 single-positive cells during necroptosis. (nih.gov)
  • Some annexins can also occur extracellularly and can have functions outside cells. (nature.com)
  • Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. (biolegend.com)
  • Annexin V binding alone cannot differentiate between apoptotic cells and necrotic. (biolegend.com)
  • For flow cytometric staining, the suggested use of this reagent is 5 µl per 100,000 - million cells in a 100 µl volume of Annexin V Binding Buffer (Cat No. 422201). (biolegend.com)
  • No. 420201) and then resuspend cells in Annexin V Binding Buffer (Cat. (biolegend.com)
  • no. 422201) is recommended for use with Annexin V staining.Annexin V binding alone cannot differentiate between apoptotic cells and necrotic. (biolegend.com)
  • 1. Wash cells twice with cold BioLegend cell staining buffer (cat # 420201) and then resuspend cells in Annexin V Binding Buffer (cat # 422201) at a concentration of 1x10e6 cells/ml. (biolegend.com)
  • Moreover, percentages of terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling-positive cells were significantly increased in 3-BrPA-treated mice, and this apoptosis-inducing efficacy was reflected in vivo by 99m Tc-hydrazinonicotinamide-Annexin V imaging. (aacrjournals.org)
  • A5 is highly expressed by cells with barrier functions, including endothelial cells and placental trophoblasts. (yu.edu)
  • Peng Y , Zhang Z , Zhang A , Liu C , Sun Y , Peng Z , Liu Y , . Membrane-cytoplasm translocation of annexin A4 is involved in the metastasis of colorectal carcinoma. (aging-us.com)
  • TMS caused a strong translocation of annexin A5 from cytosol into mitochondria . (bvsalud.org)
  • Confocal laser scanning microscopic analysis clearly showed that TMS induced translocation of annexin A5 into mitochondria . (bvsalud.org)
  • The pro-resolution mediators Annexin-A1 AnxA1 and lipoxin A4 LXA4 exert counter-regulatory effects on leukocyte recruitment, however to date, the dual-cumulative effects of these formyl peptide receptor-2 FPR2-ALX agonists in the context of human intestinal diseases are unclear. (duhnnae.com)
  • Annexin A1 binds to cellular membranes in a calcium-dependent manner, promotes membrane fusion and endocytosis, and has been implicated as an anti-inflammatory mediator. (ecmbio.com)
  • Interfering with intracellular annexin function, by overexpressing mutants or by using RNA-interference-mediated downregulation, has different effects depending on the annexin being targeted. (nature.com)
  • A common haplotype of the annexin A5 (ANXA5) gene promoter is associated with recurrent pregnancy loss. (semanticscholar.org)
  • Annexin A5 exhibits anticoagulant properties that appear to be defective in patients with antiphospholipid syndrome (APS) resulting in repeated thrombosis and recurrent pregnancy loss (RPL). (cdc.gov)
  • The frequency of annexin A5 (-1C/T) polymorphism was significantly higher in SLE related groups (p = 0.02), but it did not correlate with RPL (p = 0.57) or annexin A5 level (p = 0.5). (cdc.gov)
  • Annexin A5 plasma levels are not affected by the presence of annexin A5 (-1C/T) polymorphism. (cdc.gov)
  • Although seven other annexin genes are expressed in mouse utricles, shotgun and targeted mass spectrometry showed that none were present at levels near ANXA5 in bundles and none were upregulated in stereocilia of Anxa5-/- mice. (panoramaweb.org)
  • Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. (wikipedia.org)
  • Annexin A5 (ANXA5) has been linked with a variety of cellular functions including: endocytosis, Ca2+ transfer, anticoagulation, membrane repair and exocytosis. (gla.ac.uk)
  • Therefore the measurement of Annexin V binding to the cell surface as indicative for apoptosis has to be performed in conjunction with a dye exclusion test to establish integrity of the cell membrane. (nih.gov)
  • Membrane-bound annexins can form lateral self-assemblies that affect the mobility and organization of membrane lipids. (nature.com)
  • These and related properties enable annexins to participate in several otherwise unrelated events that range from membrane dynamics to cell differentiation and migration. (nature.com)
  • Figure 3: The regulation of membrane-actin interactions by annexin A2. (nature.com)
  • Figure 4: Annexins in membrane organization and trafficking. (nature.com)
  • Accumulating evidences suggested that Annexin A3 is closely related to various malignant tumors , and plays an important role in tumor development, metastasis , invasion and drug resistance . (bvsalud.org)
  • The antitumor efficacy of i.p. given 3-bromopyruvate (3-BrPA), a HK II inhibitor, was evaluated by measuring tumor volumes and quantifying apoptosis using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling staining and 99m Tc-hydrazinonicotinamide-Annexin V scans. (aacrjournals.org)
  • On day 2, they received [68Ga]annexin A5 PET or histofluorescence TUNEL staining. (biomedsearch.com)
  • En face staining was then performed to assess the location and size (surface area) of the lipid pool within each aortic specimen. (hokudai.ac.jp)
  • No. 422201) is recommended for use with Annexin V staining. (biolegend.com)
  • Annexin V staining in mouse cornea. (antibodies-online.com)
  • Annexin A5 depletion by siRNA led to decreased proapoptotic factors such as Bax , Bak, and annexin A5 . (bvsalud.org)
  • The annexin A5-mediated pathogenic mechanism in the antiphospholipid syndrome: role in pregnancy losses and thrombosis. (semanticscholar.org)
  • Annexin A5 is thought to have a role in the pathophysiology of the antiphospholipid syndrome (APS)-a syndrome characterised by recurrent thrombosis and pregnancy morbidity. (cdc.gov)
  • Discovery of the "Disruption of the Annexin A5 Anticoagulant Crystal Shield" concept as a mechanism of thrombosis and pregnancy loss in the antiphospholipid syndrome. (yu.edu)