A member of the annexin family that is a substrate for a tyrosine kinase, ONCOGENE PROTEIN PP60(V-SRC). Annexin A2 occurs as a 36-KDa monomer and in a 90-KDa complex containing two subunits of annexin A2 and two subunits of S100 FAMILY PROTEIN P11. The monomeric form of annexin A2 was formerly referred to as calpactin I heavy chain.
Protein of the annexin family exhibiting lipid interaction and steroid-inducibility.
A protein of the annexin family isolated from human PLACENTA and other tissues. It inhibits cytosolic PHOSPHOLIPASE A2, and displays anticoagulant activity.
Protein of the annexin family with a probable role in exocytotic and endocytotic membrane events.
Protein of the annexin family originally isolated from the electric organ of the electric ray Torpedo marmorata. It has been found in a wide range of mammalian tissue where it is localized to the apical membrane of polarized EPITHELIAL CELLS.
An annexin family member that plays a role in MEMBRANE FUSION and signaling via VOLTAGE-DEPENDENT CALCIUM CHANNELS.
A protein of the annexin family that catalyzes the conversion of 1-D-inositol 1,2-cyclic phosphate and water to 1-D-myo-inositol 1-phosphate.
Family of calcium- and phospholipid-binding proteins which are structurally related and exhibit immunological cross-reactivity. Each member contains four homologous 70-kDa repeats. The annexins are differentially distributed in vertebrate tissues (and lower eukaryotes) and appear to be involved in MEMBRANE FUSION and SIGNAL TRANSDUCTION.
A family of highly acidic calcium-binding proteins found in large concentration in the brain and believed to be glial in origin. They are also found in other organs in the body. They have in common the EF-hand motif (EF HAND MOTIFS) found on a number of calcium binding proteins. The name of this family derives from the property of being soluble in a 100% saturated ammonium sulfate solution.
Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a serine moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and serine and 2 moles of fatty acids.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Quaternary ammonium analog of ethidium; an intercalating dye with a specific affinity to certain forms of DNA and, used as diiodide, to separate them in density gradients; also forms fluorescent complexes with cholinesterase which it inhibits.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.
Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
A cell line derived from cultured tumor cells.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Extracellular vesicles generated by the shedding of CELL MEMBRANE blebs.
A family of G-protein-coupled receptors that was originally identified by its ability to bind N-formyl peptides such as N-FORMYLMETHIONINE LEUCYL-PHENYLALANINE. Since N-formyl peptides are found in MITOCHONDRIA and BACTERIA, this class of receptors is believed to play a role in mediating cellular responses to cellular damage and bacterial invasion. However, non-formylated peptide ligands have also been found for this receptor class.
Proteins prepared by recombinant DNA technology.
A short pro-domain caspase that plays an effector role in APOPTOSIS. It is activated by INITIATOR CASPASES such as CASPASE 9. Isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Organic compounds that contain technetium as an integral part of the molecule. These compounds are often used as radionuclide imaging agents.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Established cell cultures that have the potential to propagate indefinitely.
A family of intracellular CYSTEINE ENDOPEPTIDASES that play a role in regulating INFLAMMATION and APOPTOSIS. They specifically cleave peptides at a CYSTEINE amino acid that follows an ASPARTIC ACID residue. Caspases are activated by proteolytic cleavage of a precursor form to yield large and small subunits that form the enzyme. Since the cleavage site within precursors matches the specificity of caspases, sequential activation of precursors by activated caspases can occur.
Fluorescent probe capable of being conjugated to tissue and proteins. It is used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.
An in situ method for detecting areas of DNA which are nicked during APOPTOSIS. Terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 prime OH ends of either single- or double-stranded DNA. The terminal deoxynucleotidyl transferase nick end labeling, or TUNEL, assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA FRAGMENTATION.
A product of the lysis of plasminogen (profibrinolysin) by PLASMINOGEN activators. It is composed of two polypeptide chains, light (B) and heavy (A), with a molecular weight of 75,000. It is the major proteolytic enzyme involved in blood clot retraction or the lysis of fibrin and quickly inactivated by antiplasmins.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Experimentally induced chronic injuries to the parenchymal cells in the liver to achieve a model for LIVER CIRRHOSIS.
The genetic complement of an organism, including all of its GENES, as represented in its DNA, or in some cases, its RNA.
Databases devoted to knowledge about specific genes and gene products.
The physiological renewal, repair, or replacement of tissue.
Liver disease in which the normal microcirculation, the gross vascular anatomy, and the hepatic architecture have been variably destroyed and altered with fibrous septa surrounding regenerated or regenerating parenchymal nodules.
Genetic loci associated with a QUANTITATIVE TRAIT.
The profession of writing. Also the identity of the writer as the creator of a literary production.
Collections of facts, assumptions, beliefs, and heuristics that are used in combination with databases to achieve desired results, such as a diagnosis, an interpretation, or a solution to a problem (From McGraw Hill Dictionary of Scientific and Technical Terms, 6th ed).
The guidelines and policy statements set forth by the editor(s) or editorial board of a publication.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
Antibodies produced by a single clone of cells.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
A plasma protein that circulates in increased amounts during inflammation and after tissue damage.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
A type of FLUORESCENCE SPECTROSCOPY using two FLUORESCENT DYES with overlapping emission and absorption spectra, which is used to indicate proximity of labeled molecules. This technique is useful for studying interactions of molecules and PROTEIN FOLDING.

Differential effects of annexins I, II, III, and V on cytosolic phospholipase A2 activity: specific interaction model. (1/23)

Annexins (ANXs) are a family of proteins with calcium-dependent phospholipid binding properties. Although inhibition of phospholipase A2 (PLA2) by ANX-I has been reported, the mechanism is still controversial. Previously we proposed a 'specific interaction' model for the mechanism of cytosolic PLA2 (cPLA2) inhibition by ANX-I [Kim et al., FEBS Lett. 343 (1994) 251-255]. Here we have studied the cPLA2 inhibition mechanism using ANX-I, N-terminally deleted ANX-I (DeltaANX-I), ANX-II, ANX-II(2)P11(2), ANX-III, and ANX-V. Under the conditions for the specific interaction model, ANX-I, DeltaANX-I, and ANX-II(2)P11(2) inhibited cPLA2, whereas inhibition by ANX-II and ANX-III was negligible. Inhibition by ANX-V was much smaller than that by ANX-I. The protein-protein interactions between cPLA2 and ANX-I, DeltaANX-I, and ANX-II(2)P11(2) were verified by immunoprecipitation. We can therefore conclude that inhibition of cPLA2 by specific interaction is not a general function of all ANXs, and is rather a specific function of ANX-I. The results are consistent with the specific interaction model.  (+info)

Ca(2+) and membrane binding to annexin 3 modulate the structure and dynamics of its N terminus and domain III. (2/23)

Annexin 3 (ANX A3) represents approximately 1% of the total protein of human neutrophils and promotes tight contact between membranes of isolated specific granules in vitro leading to their aggregation. Like for other annexins, the primary molecular events of the action of this protein is likely its binding to negatively charged phospholipid membranes in a Ca(2+)-dependent manner, via Ca(2+)-binding sites located on the convex side of the highly conserved core of the molecule. The conformation and dynamics of domain III can be affected by this process, as it was shown for other members of the family. The 20 amino-acid, N-terminal segment of the protein also could be affected and also might play a role in the modulation of its binding to the membranes. The structure and dynamics of these two regions were investigated by fluorescence of the two tryptophan residues of the protein (respectively, W190 in domain III and W5 in the N-terminal segment) in the wild type and in single-tryptophan mutants. By contrast to ANX A5, which shows a closed conformation and a buried W187 residue in the absence of Ca(2+), domain III of ANX A3 exhibits an open conformation and a widely solvent-accessible W190 residue in the same conditions. This is in agreement with the three-dimensional structure of the ANX A3-E231A mutant lacking the bidentate Ca(2+) ligand in domain III. Ca(2+) in the millimolar concentration range provokes nevertheless a large mobility increase of the W190 residue, while interaction with the membranes reduces it slightly. In the N-terminal region, the W5 residue, inserted in the central pore of the protein, is weakly accessible to the solvent and less mobile than W190. Its amplitude of rotation increases upon binding of Ca(2+) and returns to its original value when interacting with membranes. Ca(2+) concentration for half binding of the W5A mutant to negatively charged membranes is approximately 0.5 mM while it increases to approximately 1 mM for the ANX A3 wild type and to approximately 3 mM for the W190 ANX A3 mutant. In addition to the expected perturbation of the W190 environment at the contact surface between the protein and the membrane bilayer, binding of the protein to Ca(2+) and to membranes modulates the flexibility of the ANX A3 hinge region at the opposite of this interface and might affect its membrane permeabilizing properties.  (+info)

Calcyclin, a Ca2+ ion-binding protein, contributes to the anabolic effects of simvastatin on bone. (3/23)

In vitro treatment with a pharmacological dose of simvastatin, a potent pro-drug of a 3-hydroxy-3-methylglutaryl-coenzyme A reductase inhibitor, stimulates bone formation. In our study, simvastatin stimulated differentiation of osteoblasts remarkably in a dose-dependent manner, with minimal effect on proliferation. To identify the mediators of the anabolic effects of simvastatin on osteoblasts, we tried to identify and characterize simvastatin-induced proteins by using proteomic analysis. Calcyclin was significantly up-regulated by more than 10 times, and annexin I was also up-regulated by simvastatin. However, annexin III, vimentin, and tropomyosin were down-regulated. Up-regulated calcyclin mRNA by simvastatin was validated by reverse transcription in mouse calvarial cells. In confocal microscope analysis, green fluorescence protein-calcyclin fusion protein was ubiquitously observed in the of MC3T3-E1 cells transfected with green fluorescence protein-calcyclin cDNA containing plasmid and was quickly concentrated in the nucleus 20 min after simvastatin treatment. Overexpression of calcyclin cDNA stimulated both the proliferation and expression of alkaline phosphatase mRNA significantly, without exposure to simvastatin in MC3T3-E1 cells. However, both the rate of proliferation of the osteoblasts and the expression of alkaline phosphatase mRNA were suppressed significantly 1 day after treatment with the calcyclin-specific small interference RNA, and furthermore, simvastatin did not overcome this suppression in the small interference RNA-pretreated MC3T3-E1 cells. In conclusion, calcyclin is one of the candidate proteins that plays a role in osteoblastogenesis in response to simvastatin, although the precise functions of calcyclin in osteoblast remain to be verified.  (+info)

Isolated small rat hepatocytes express both annexin III and terminal differentiated hepatocyte markers, tyrosine aminotransferase and tryptophan oxygenase, at the mRNA level. (4/23)

We recently showed that annexin III is expressed in isolated small rat hepatocytes but, not in parenchymal hepatocytes. In the present study, we used reverse transcription polymerase chain analysis to examine the annexin III mRNA level in isolated small rat hepatocytes and parenchymal hepatocytes. Annexin III mRNA was detected in isolated small hepatocytes, but not in isolated parenchymal hepatocytes, confirming the presence of annexin III expression in isolated small rat hepatocytes at the mRNA level and indicating that the absence of annexin III expression in isolated parenchymal hepatocytes is due to the absence of annexin III mRNA. Furthermore, we examined the mRNA level of tyrosine aminotransferase and tryptophan oxygenase, two terminally differentiated hepatocyte markers. mRNA for these markers was detected in both parenchymal hepatocytes and small hepatocytes.  (+info)

Expression of annexin A3 in primary cultured parenchymal rat hepatocytes and inhibition of DNA synthesis by suppression of annexin A3 expression using RNA interference. (5/23)

Annexin A3 is a member of the lipocortin/annexin family, which binds to phospholipids and membranes in a Ca(2+)-dependent manner. Although annexin A3 has various functions in vitro, its cellular significance is completely unknown. Annexin A3 is not found in rat liver in vivo. In the present study, we investigated the expression of annexin A3 in primary cultured parenchymal rat hepatocytes. Annexin A3 protein was detected in 48-h, but not 2.5-h, cultured hepatocytes using Western blot analysis. The annexin A3 level further increased after an additional 24 h of culture. Annexin A3 mRNA was not detected in 2.5-h cultured hepatocytes but was detected 22 h after the start of culture by RT-PCR analysis, reaching a maximum value after 48 h of culture. To define the role of Annexin A3 in DNA synthesis, RNA interference was used to reduce annexin III gene expression in hepatocytes. The transfection of small interfering RNAs targeting annexin A3 in the hepatocytes reduced the corresponding mRNA and protein expression by approximately 80% and more than 90%, respectively, at 24 h after transfection. In the annexin A3 small interfering RNAs-transfected cells, DNA synthesis, as assessed by [3H]thymidine incorporation, decreased by approximately 70% not only in the control cultures, but also in the hepatocyte growth factor- or epidermal growth factor-treated cells. These findings show that annexin A3 is expressed in primary cultured parenchymal rat hepatocytes and that the suppression of annexin A3 expression using RNA interference inhibits DNA synthesis.  (+info)

Change in annexin A3 expression by regulatory factors of hepatocyte growth in primary cultured rat hepatocytes. (6/23)

We have recently reported that annexin (Anx) A3 expression is necessary for hepatocyte growth in cultured rat hepatocytes seeded at half the subconfluent density on collagen. In the present study, we investigated the effects of various regulatory factors of hepatocyte growth on AnxA3 expression. AnxA3 expression was significantly reduced in hepatocytes cultured under various growth inhibitory conditions such as presence of dexamethasone, culture at subconfluent cell density, and on EHS-Matrigel and lactose-carrying styrene polymer. On the other hand, hepatocyte growth factor and epidermal growth factor, stimulators of hepatocyte growth, significantly increased AnxA3 expression in hepatocytes cultured on EHS-Matrigel. These results show close correlation between known stimulatory or inhibitory actions of various factors to hepatocyte growth and increase or decrease in AnxA3 expression, and suggest the involvement of AnxA3 in their regulation of hepatocyte growth.  (+info)

Annexin A3-expressing cellular phenotypes emerge from necrotic lesion in the pericentral area in 2-acetylaminofluoren/carbon tetrachloride-treated rat livers. (7/23)

Recently we found a small hepatocyte-specific protein, annexin A3 (AnxA3), in fractionated adult rat hepatocytes. Here we describe the results of an in vivo demonstration of AnxA3-expressing cellular phenotypes in the liver with 2-acetylaminofluoren (2-AAF)/carbon tetrachloride (CCl(4))-injury. In association with an elevation of alanine amino transferase (ALT) and aspartic acid amino transferase (AST) activities, hepatic AnxA3 mRNA increased markedly. AnxA3-positive cells were detected in clustered cells present in or emerging from the pericentral region. These albumin-expressed cells were histologically similar to cells expressing CD34, a hematopoietic cell marker protein. The number of clusters decreased in the days following CCl(4) treatment, and annexin-negative, but albumin-positive, oval cells appeared. We concluded that the agent-induced liver defect initially recruits bone marrow-derived cells, and that it promotes differentiation of these cells into AnxA3-positive cells, followed by emergence of the oval cells, which might have a role in the restitution of the damaged liver.  (+info)

Annexin A3 expression after stroke in the aged rat brain. (8/23)

In an effort to identify new proteins involved in functional recovery after cerebral ischemia, young (3 months) and aged (18 months) male rats were subjected to middle cerebral artery (MCA) occlusion. Brains were harvested at 3- and 14-days post ischemia and proteins from the peri-infarcted and the corresponding contralateral area and total proteins were analyzed by two-dimensional polyacrylamide gel electrophoresis followed by mass spectrometry analysis. Annexin A3 (ANXA3) was identified as one upregulated protein in the post-ischemic rat brain. Using western blotting, real-time PCR and immunohistochemistry, we confirmed that at 3-14 days post-stroke, ANXA3 expression in the peri-infarct area was consistently increased over the corresponding area of control rats. Double staining revealed that ANXA3 is produced by activated microglial cells. We found that aged rats also had more newly proliferating cells expressing ANXA3 than young rats do. Occasionally, ANXA3-immunopositive cells wraped around neurons, suggesting that annexin A3 may be involved in the removal of dying neurons after stroke.  (+info)

Mouse anti Human Annexin A3 antibody, clone 4F1 recognizes human annexin A3, also known as Annexin III, Inositol 1,2-cyclic phosphate 2-ph
The invention provides a novel method for the chemical synthesis of 2′,3′-cyclic phosphate and phosphorothioate of mono and terminated oligonucleotides synthesis. The invention also provides a novel method of for the chemical synthesis of 2′,3′- and 3′,5′-cyclic phosphate and phosphorothioate mononucleotide nucleotides. The process is based on quick and efficient cyclization of phosphoramidate moiety and neighboring hydroxyl group. The present invention is directed towards the synthesis of high purity DNA and RNAs, specifically to introduce cyclic phosphate at 3′-end of oligonucleotides. Such DNA and RNAs have extensive application in therapeutics, diagnostics, drug design, and selective inhibition of an RNA sequence within cellular environment, in pre-tRNA cleavage and in ribozyme ligation. The 2′,3′-cyclic phosphate nucleosides are involved in a vast number of applications in molecular biology in general and mammalian cells in particular. The invention also envisions providing kits
Annexin III antibody [1A9] (Annexin A3) for FACS, WB. Anti-Annexin III mAb (GTX84883) is tested in Human, Dog, Monkey, Rat samples. 100% Ab-Assurance.
BACKGROUND AND OBJECTIVE: Proteinuria assessment is key in investigating chronic kidney disease (CKD) but uncertainty exists regarding optimal methods. Albuminuria, reflecting glomerular damage, is usually measured, but non-albumin proteinuria (NAP), reflecting tubular damage, may be important. This study investigated the prevalence and associations of albuminuria and NAP, and the optimum number of urine specimens required. METHODS: 1,741 patients with CKD stage 3, recruited from primary care, underwent medical history, clinical assessment, blood sampling, and submitted three early morning urine samples for albumin to creatinine ratio (uACR) and protein to creatinine ratios (uPCR). Albuminuria was defined as uACR ≥ 3 mg/mmol in at least two of three samples. Isolated NAP was defined as uPCR ≥ 17 mg/mmol in two of three samples and uACR ,3 mg/mmol in all three. Prevalence and associations of albuminuria and NAP, degree of agreement between single uACR and average of three uACRs, and urine ...
This invention relates to benzodiazepine derivatives which are useful as drugs exhibiting antagonism at the gastrin and/or CCK-B receptor, and to their production.
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Previous studies in our laboratory identified that 3-deazaneplanocin A (DZNep), a carbocyclic adenosine analog and histone methyl transferase inhibitor, suppresses TGFβ-induced epithelial-to-mesenchymal (EMT) characteristics. In addition, DZNep epigenetically reprograms miRNAs (miRs) to regulate endogenous TGFbeta1 levels via miR-663/4787 mediated RNA interference (Mol Cancer Res. 2016 Sep 13. pii: molcanres.0083.2016) (1). While DZNep also attenuates exogenous TGFbeta-induced EMT response, the mechanism of this inhibition was unclear. Here, DZNep induced miR-202-5p to target both TGFβ receptors, TGFBR1 and TGFBR2, for RNA interference and thereby contribute to the suppression of exogenous TGFbeta-induced EMT in pancreatic cancer cells. Lentiviral overexpression of miR-202 significantly reduced the protein levels of both TGFbeta receptors and suppressed TGFbeta signaling and EMT phenotypic characteristics of cultured parenchymal pancreatic cancer cells. Consistently, transfection of anti-miRs ...
This page contains information on the chemical 1,3-Propanediol, 2-(hydroxymethyl)-2-isopropyl-1-methyl-, cyclic phosphate (1:1) including: 5 synonyms/identifiers.
In my area there were no mice available so I gave her a small rat. She started eating it (her 1st piece of food in weeks) but then I made the mistake to try and take her water bowl so I can refresh her water and she spat out the rat and after that (that was like 4 weeks ago) she has not even shown interest in food at all. I heard that it can be that she is in hibernation that she does not eat so much anymore but 4 weeks is a long time and believe you me I wiggle that dead mouse around as if it was doing the Macarena! Can anyone please help as to what the problem can be? I am at the point of total cluelessness ...
Valerian Rat 3 variations available; Small, Large and Fluffy Each rat is filled with valerian, a highly attractive herb to most cats. Very similar to catnip but stronger smelling and potent for you kitties fun. Great toy to keep your kitty active and take some of that bordom away.These are handmade and as such the sizes do vary and size given is only a rough guide as they can be slightly bigger or smaller that listed Small Rat - 20cm longLarge Rat - 30cm longFluffy Rat - 15cm long Please note that you will receive a random colour from the colours available. If you require a specific colour please message beforehand to make sure I have stock of it
No one should take this vaccine-it is one of the most dangerous vaccines ever devised. No citation given. I find it ironic that someone who wants everyone to believe him at face value provides no scientific support to back up his arguments. Oh, maybe thats because no such thing exists. Another argument put forth by supporters of all this paranoia is that this vaccine hasnt been sufficiently tested to use on humans. If thats so, how can we legitimately claim it to be dangerous? It contains an immune adjuvant called squalene (MF-59) which has been shown to cause severe autoimmune disorders such as multiple sclerosis, rheumatoid arthritis, and lupus. Again, no citation given. There is no medical evidence linking squalene to multiple sclerosis. Not a single citation in the whole universe of medical literature. What about RA? There are a couple of small rat studies show moderate increases in inflammatory mediators after injection with straight squalene (which does not happen with the vaccine; its ...
In my area there were no mice available so I gave her a small rat. She started eating it (her 1st piece of food in weeks) but then I made the mistake to try and take her water bowl so I can refresh her water and she spat out the rat and after that (that was like 4 weeks ago) she has not even shown interest in food at all. I heard that it can be that she is in hibernation that she does not eat so much anymore but 4 weeks is a long time and believe you me I wiggle that dead mouse around as if it was doing the Macarena! Can anyone please help as to what the problem can be? I am at the point of total cluelessness ...
The present invention relates to novel chemical compounds, methods for their discovery, and their therapeutic use. In particular, the present invention provides benzodiazepine derivatives and methods
Batî , gun, gunik an jî hêlik (bi latînî: Testiculus), organek e zayendî an cinsî ye li ba candarên nêr û mirovan e. Ciyê batî di bin kîrê mêr û bin organa zayendî yên gelek canewerên da dikeve.Wezîfa an erka bingehîn ya batiyan ew e, ku tov (avik, sperm) tê de peyda bikin. Hormoneke zayendî bi navê testosteronê tê de têne der kirin.. ...
Nephrotic syndrome recurred in 16 patients who had been free of the disease for an interval of 4 to 25 years. Their initial illness began in childhood and responsed to adrenocorticosteroid therapy; in 10 patients it was associated with few changes on light microscopic examination of kidney biopsy specimens. The late recurrence also responded to steroids, and in only 1 patient did low-grade proteinuria persist after treatment. Renal biopsy specimens taken from 8 patients during late recurrence showed minimal-change nephrotic syndrome in 7 and focal segmental glomerulosclerosis in 1. Renal function remained normal in all patients. The late recurrence of minimal-change nephrotic syndrome has most of the features of that syndrome seen in the more typical childhood age group and should be managed similarly. ...
The design and total synthesis of DL-6-deoxy-6-(hydroxymethyl)-scyllo-inositol 1:7-cyclic 2,4-trisphosphate (4), a conformationally restricted cyclic phosphate analogue of the second messenger inositol 1,4,5-trisphosphate [Ins(1,4,5)P 3 ], is described. The protected inosose 2,4,6/3,5-pentahydroxy-3,5-bis-O-(p-methoxybenzyl)-2,4,6-O- methylidynecyclohexanone (7) was obtained from myoinositol orthoformate in two steps, and Wittig methylenation and then hydroboration-oxidation using 9-BBN-H/OH - /H 2 O 2 gave the axial hydroxymethyl derivative 9. A series of protection/ deprotection steps provided the diol 13, which was converted into two cyclic phosphate esters 14a and 14b, epimeric at phosphorus, by reaction with (benzyloxy)bis(N,N-diisopropylamino)phosphine/ 1H-tetrazole followed by m-CPBA. Two other hydroxyl groups were then exposed and phosphorylated, and total deprotection gave racemic 4. NMR studies confirmed that in 4 the phosphate group equivalent to the 4-phosphate of Ins(1,4,5)P 3 is held in
Fletazepam[1] is a drug which is a benzodiazepine derivative. It has sedative and anxiolytic effects similar to those produced by other benzodiazepine derivatives, but is mainly notable for its strong muscle relaxant properties.[2] Fletazepam is most closely related to other N-trifluoroethyl substituted benzodiazepines such as halazepam and quazepam.[3] ...
Its a full-grown Ball Python, which is no threat to anything bigger than a small rat. They love to soak up your body warmth. If it was in a bra, it would be curled up in a ball, so one cup would be noticeably larger -- unless there were two of them, in which case the Polish police are in for another surprise. ...
https://www.officialbenzofury.net/products/Flubromazolam.html Flubromazolam is a brand new Benzodiazepine derivative available to buy @ Offical Benzo Fury. Official Benzo Fury is the first ... Write your own product review ... Flubromazolam - Why is it Such a Popular Research ... - ...
These enzymes form some of the cyclic phosphate Ins(cyclic1,2)P(4,5)P2 as well as Ins(1,4,5)P3. They show activity towards phosphatidylinositol, i.e., the activity of EC 4.6.1.13, phosphatidylinositol diacylglycerol-lyase, in vitro at high [Ca2+]. Four beta-isoforms regulated by G-proteins, two gamma-forms regulated by tyrosine kinases, four delta-forms regulated at least in part by calcium and an epsilon-form, probably regulated by the oncogene ras, have been found ...
Information about the open-access article Hepatocellular carcinoma: microstructure and expression features of hepatocyte marker, alphafetoprotein, cytokeratins 7 and 20 in DOAJ. DOAJ is an online directory that indexes and provides access to quality open access, peer-reviewed journals.
PromoCells facility remains operational and we continue to support researchers worldwide. We are proud of making a contribution to the fight against SARS-CoV-2 with our products. We are here to help - contact us. Dismiss. ...
The unfolded protein response (UPR) is an evolutionarily conserved mechanism whereby cells respond to stress conditions that target the endoplasmic reticulum (ER). One of the major targets of the UPR is the 78kDa Glucose Regulated Protein Grp78 (BiP). The transcriptional activation of the promoter of GRP78 has been used extensively as an indicator of the onset of the UPR. The transcriptional activation of Grp78 in response to ER stress has been well documented. It is characterized by multiple transcription factors such as YY1, TFII-I, ATF6(N), and NF-Y binding to conserved promoter sequences at the onset of ER stress.; There are also epigenetic changes that occur during the activation of the Grp78 promoter. We have observed ER-stress induced binding of the histone acetyltransferase p300 to the Grp78 promoter and histone H4 acetylation. We have also seen the arginine methyltransferase PRMT1, and evidence of its action through methylation of the arginine 3 residue on histone H4. We show the ...
shark at sam.neosoft.com (John D. Valentich) wrote: , , We have recently started preparing polyclonal antibodies in rabbits against synthetic peptides , from annexins and phospholipase A2 activating protein (PLAP). Peptides were , conjugated to the carrier BSA. We are wondering about the problem of , antibodies generated against BSA reacting with serum albumin or albumin-like , epitopes in other cellular proteins. Questions: , , 1. Is it likely (or inevitable) that our antisera will contain high titer , antibodies against BSA? Could they be expected to cross react with other , non-albumin cellular proteins? They will almost certainly react with albumin. , , 2. If so, is the best (or only?) solution to this problem affinity purification of annexin or PLAP , antibodies from the antisera? Affinity purification should work. You could also adsorb the antisera with BSA and hope to remove many of the cross-reacting antibodies. Its probably too late now but you can use keyhole limpet hemocyanin (KLH) ...
Tpt1 is an essential 230-amino-acid enzyme that catalyzes the final step in yeast tRNA splicing: the transfer of the 2-PO4 from your splice junction to NAD+ to form ADP-ribose 1-2cyclic phosphate and nicotinamide. (Greer et al. 1983; Apostol et al. 1991; Sawaya et al. 2003). Third, the 2-PO4 at the splice junction MYH11 is usually … Continue reading Tpt1 is an essential 230-amino-acid enzyme that catalyzes the final step. ...
Clur sa etal. The pcwp of 24mmhg, in sum. 4. Assumptions: Describe some underlying problem (such as promethazine and metoclopramide) include somnolence, nervousness, irritability, and an event known as idiopathic nephrosis, childhood nephrosis, or minimal-change nephrotic syndrome (centers for disease control and a thoracolumbosacral orthosis (tlso), which is more than 69. Although the effects of untreated pain in children, under most circumstances. G. , increased vascular tone, and muscle strength that ultimately occurs in tbw, with the carbohydrate content is higher and more compressible than bones in the dr usually at 21years of age. Allowing it to be largely a part of the fhr deceleration is delayed until atrial contractions occur simultaneously with mmr, religion and spirituality are associated with the intimal plaque. With the completion of these bones to accommodate different canal widths. As child nears discharge, arrange for someone to remain silent, which means finish 4 minutes and ...
Donors of H2S may be beneficial in treating cardiovascular diseases where the plasma levels of H2S are decreased. Therefore, we investigated the mechanisms involved in relaxation of small arteries induced by GYY4137 [(4-methoxyphenyl)-morpholin-4-yl-sulfanylidene-sulfido-λ5-phosphane;morpholin-4-ium], which is considered a slow-releasing H2S donor. Sulfides were measured by use of 5,5′-dithiobis-(2-nitro benzoic acid), and small rat mesenteric arteries with internal diameters of 200-250 µm were mounted in microvascular myographs for isometric tension recordings. GYY4137 produced similar low levels of sulfides in the absence and the presence of arteries. In U46619-contracted small mesenteric arteries, GYY4137 (10−6-10-3 M) induced concentration-dependent relaxations, while a synthetic, sulfur-free, GYY4137 did not change the vascular tone. L-cysteine (10−6-10-3 M) induced only small relaxations reaching 24 ± 6% at 10-3 M. Premixing L-cysteine (10-3 M) with Na2S and GYY4137 decreased Na2S ...
TY - JOUR. T1 - Profiling proteinuria in pediatric patients. AU - Abitbol, Carolyn L.. AU - Chandar, Jayanthi. AU - Onder, Ali Mirza. AU - Nwobi, Obioma. AU - Montané, Brenda. AU - Zilleruelo, Gastón. PY - 2006/7/1. Y1 - 2006/7/1. N2 - This study was designed to characterize proteinuria in children with kidney disease. Random urine samples from 250 pediatric patients were examined by quantitative measures of total protein (pr), albumin (Alb), and creatinine (cr). Patient diagnoses were subjectively categorized as Glomerular (GD) or Tubulo-interstitial disease (TD) in origin. Proteinuria was quantitated by the random urine protein-to-creatinine (Upr/cr) ratio, and glomerular proteinuria was assessed as the albumin-to-creatinine ratio (Ualb/cr) and percentage albuminuria (%Alb=Alb/pr* 100). The non-albumin fraction (1-Alb/ pr) includes low-molecular-weight proteins and micro- and macroglobulins. Of the 250 patients, 112 (45%) had GD and 138 (55%) had TD. Both proteinuria and albuminuria ...
The pHi (intracellular pH) is an important physiological parameter which is altered during hypoxia and ischaemia, pathological conditions accompanied by a dramatic decrease in pHi. Sensors of pHi include ion transport systems which control intracellular Ca2+ gradients and link changes in pHi to functions as diverse as proliferation and apoptosis. The annexins are a protein family characterized by Ca2+-dependent interactions with cellular membranes. Additionally, in vitro evidence points to the existence of pH-dependent, Ca2+-independent membrane association of several annexins. We show that hypoxia promotes the interaction of the recombinant annexin A2-S100A10 (p11) and annexin A6 with the plasma membrane. We have investigated in vivo the influence of the pHi on the membrane association of human annexins A1, A2, A4, A5 and A6 tagged with fluorescent proteins, and characterized this interaction for endogenous annexins present in smooth muscle and HEK (human embryonic kidney)-293 cells ...
Ro48-6791 is a drug, a benzodiazepine derivative developed by Hoffman-LaRoche in the 1990s. Ro48-6791 was developed as an alternative to the short-acting imidazobenzodiazepine midazolam, for use in induction of anaesthesia and conscious sedation for mino
The tranquilizers - benzodiazepine derivatives are widely used, although this method is also associated with a number of adverse reactions, including: reduce of the ability to concentrate, coordination disorders and so on. At the same time, the patients often develop drug dependence. In certain cases it is advisable to use psychotherapeutic drugs, but on the one hand the deficiency of well qualified specialists and on the other hand, the patients fear of nervous profile physicians, determine the inadequate treatment. In such cases and also during the mild vegetative nervous system disorders, the herbal remedies are widely used, one of the most important and effective drugs is Vamelan. ...
This study is one of the largest single-center studies to investigate the course and response to treatment in adult patients with MCD. The treatment of MCD in adults is challenging for several reasons. First, because so many patients respond to initial therapy and because MCD is believed to have a benign course, there are no controlled treatment trials in adults. Data are extrapolated from childhood MCD. Second, the pathogenesis of MCD remains unknown. A variety of immunologic abnormalities that affect both humoral and cell-mediated immunity have been described in affected individuals. T cell activation and resultant cytokine or permeability factor mediated injury to the glomerular foot processes has been proposed as a major contributor to disease pathogenesis (9). There is an association of disease onset with viral infections, allergies, malignancy, and numerous medications, but the exact triggers are also unknown. Therefore, our limited understanding of the disease makes it difficult to ...
This graph shows the total number of publications written about Annexin A3 by people in this website by year, and whether Annexin A3 was a major or minor topic of these publications ...
Victims of human trafficking are forced to pass two sets of court proceedings so as to claim their rights. This can take years and causes additional trauma, while the outcome is uncertain, so most victims decide not to claim damages
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Tifluadom is a benzodiazepine derivative with an unusual activity profile. Unlike most benzodiazepines, tifluadom has no activity at the GABAA receptor, but instead is a selective agonist for the κ-opioid receptor. In accordance, it has potent analgesic and diuretic effects in animals, and also has sedative effects and stimulates appetite ...
Invitrogen™ eBioscience™ Annexin V, Recombinant Protein Unlabeled; 300μg Invitrogen™ eBioscience™ Annexin V, Recombinant Protein...
Annexin A2兔多克隆抗体(ab90643)可与人样本反应并经WB, IHC实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
Get Free Gelatin-Based Coating Solution per primary cell order, which can be used for most primary cell culture from Cell Biologics through 2018. ...
Get Free Gelatin-Based Coating Solution per primary cell order, which can be used for most primary cell culture from Cell Biologics through 2018. ...
The GWM-IE boiler offers an efficiency of up to 95% AFUE, meaning it converts up to 95% of its fuel into useable heat. Fuel flexibility lets you use either natural or LP gas as a heat source. Nothings more efficient. With an efficiency of up to 95% AFUE, the GWM-IE boiler can mean several hundred dollars per year in energy savings.This product ...
Flurazepam (marketed under the brand names Dalmane and Dalmadorm) is a drug which is a benzodiazepine derivative. It possesses anxiolytic, anticonvulsant, sedative and skeletal muscle relaxant properties. It produces a metabolite with a very long half-life (40â€250 hours), which may stay in the bloodstream for up to four days.[1] Flurazepam is therefore unsuitable as a sleeping medication for some individuals due to next day sedation. ...
In our hands, the trypsin actually reduced the Annexin staining presumably by stripping off all the phosphotidyl-serine on the surface along with the surface proteins (adhesion molecules). The only time we have seen enhanced annexin is when the cells suffer enough trauma in harvesting that the membrane is not intact and the Annexin gets inside the cell and attaches to the ps on the inner membrane. Julie At 02:11 PM 8/18/98, you wrote: , ,I have a client who is staining with Annexin-FITC. They are looking at ,cell lines and are using trypsin to get the cells off the flasks. We are ,seeing what we think is an exagerated Annexin response. Does anyone out ,there have any feedback on how trypsin affects Annexin staining? Any ,experience and or information about this will be greatly appreciated. , Thanks in advance, , Joan Kalnitsky ,Joan Kalnitsky ,Flow Cytometry Laboratory ,VMRCVM ,(540) 231-4115 ,FAX 540-231-7367 , , It is better to serve than to receive , B. Borg , , , , , ...
Abstract: The use of primary cells as a model system is expanding and so are the protocols in which they are used. Primary cells can more closely mimic an in vivo-like state and generate more physiologically relevant data than immortalized cells. But unlike immortalized cells, primary cells have complex nutritional needs and require optimized growth conditions.. In this webinar you will learn:. ...
Abstract: The use of primary cells as a model system is expanding and so are the protocols in which they are used. Primary cells can more closely mimic an in vivo-like state and generate more physiologically relevant data than immortalized cells. But unlike immortalized cells, primary cells have complex nutritional needs and require optimized growth conditions.. In this webinar you will learn:. ...
Primary Cells and Media: Broad donor variety, normal and diseased cells, technical support. Rely on Lonza to overcome your primary cell culture challenges.
Halazepam is a benzodiazepine derivative. It possesses anxiolytic, anticonvulsant, sedative and skeletal muscle relaxant properties. It is a trifluoromethyl derivative of nordazepam. While its structure may be similar to chlordiazepoxide and diazepam, it has both less toxicity and less tendency to cause paradoxical hostility and aggression than either of them. Halazepam is no longer marketed in the United States, and was withdrawn by Schering-Plough due to poor sales. [Wikipedia]
prazepam 10mg is a benzodiazepine derivative drug developed by Warner-Lambert in the 1960s. It possesses anxiolytic, anticonvulsant, sedative and skeletal muscle relaxant properties. Prazepam is a prodrug for desmethyldiazepam which is responsible for the therapeutic effects of prazepam
A novel protein target of mouse calcyclin (S100A6) was detected by a gel overlay method with 125I-labelled calcyclin. Interaction of calcyclin with its 30 kDa target protein (p30) present in Ehrlich ascites tumour (EAT) cells depended on the presence of Ca2+ ions. The binding of p30, evidenced by the reaction with 125I-labelled calcyclin, was found to be of higher affinity than the binding between mouse calcyclin and annexin II or glyceraldehyde-3-phosphate dehydrogenase. Examination of tissue extracts by the gel overlay method has shown that p30 is present not only in the EAT cells but also in mouse brain and spleen. This novel target protein of mouse calcyclin was purified to homogeneity from EAT cells by means of Phenyl-Sepharose chromatography, affinity chromatography and CM-cellulose chromatography. Purified p30 was digested with α-chymotrypsin and a partial amino acid sequence of one of the resulting peptides was established. A database search analysis revealed that the sequence is ...
Human Kidney Primary Cell Culture Free Expansion Media Serum Free .. This product is also available with Serum Cat# M36059-01ES. This product would require pre-coated flasks with Human Kidney Primary Cell Culture Extra-cellular Expansion Matrix Cat# E36059-01 and Human Kidney Primary Cell Cultures Cat# 36059-01. This product is tissue culture tested including Stem Cells and is available as 500ml sterile filtered unit.. The product is also available as a pack of 6, 500ml unit sizes.. ...
ANXA4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 321 amino acids (1-321 a.a.) and having a molecular mass of 36 kDa.
ATCC offers media and supplements, easy-to-use cell dissociation reagents, and hassle-free cryopreservation media optimized for culturing primary cells.
Annexin A5 forms a shield around negatively charged phospholipid molecules, thus reducing their availability for coagulation. ... Thus, anti-annexin A5 antibodies increase phospholipid-dependent coagulation steps. The lupus anticoagulant antibodies are ...
... which encodes annexin A5. It is also neighbored by PP12613 located on the positive strand. The gene on chromosome 4 encoding ...
Annexin A5 affinity assay, a test for cells undergoing apoptosis, often uses flow cytometry Cell cycle analysis Coulter counter ...
Annexin A3 MeSH D12.776.157.125.050.080 - annexin a4 MeSH D12.776.157.125.050.100 - annexin a5 MeSH D12.776.157.125.050.110 - ... annexin a6 MeSH D12.776.157.125.050.120 - annexin a7 MeSH D12.776.157.125.412.249 - calmodulin MeSH D12.776.157.125.412.311 - ... annexin a1 MeSH D12.776.157.125.050.060 - annexin a2 MeSH D12.776.157.125.050.070 - ...
... annexin A3 MeSH D08.811.277.352.640.125 - 3',5'-cyclic-GMP phosphodiesterase MeSH D08.811.277.352.640.150 - 3',5'-cyclic- ... cytochromes a3 MeSH D08.244.187.249 - cytochromes b MeSH D08.244.187.500 - cytochromes b5 MeSH D08.244.187.600 - cytochromes b6 ...
... a sequence for the insulin gene Adenosine A3 receptor, a human gene Annexin A3, a human gene ATC code A03 Drugs for functional ... A3, A03 or A.III may refer to: A3 paper, a paper size defined by ISO 216 A3 regulatory sequence, ... a band known as A3 in the U.S. to avoid confusion with the country group Alabama A-3, a Yamaha musical instrument product A3 ... a British Isles plants community Gibberellin A3, a plant hormone HLA-A3, a Human MHC Serotype HLA-A Subfamily A3, a rhodopsin- ...
... regulatory sequence in biochemistry A5, the abbreviation for the androgen Androstenediol Annexin A5, a human cellular ... A5 is the code for permission to use specific land or premises for takeaways A5/1, A5/2 and A5/3, ciphers used in cellular ... the alternating group on five elements Apple A5, the Apple mobile microprocessor ARM Cortex-A5, ARM applications processor A5 ... a common shorthand name for the Browning Auto-5 shotgun Gibson A-5 mandolin, a Gibson mandolin Tippmann A-5, a semi-automatic ...
This clustering of annexin A5 on the membrane greatly increases the intensity of annexin A5 when labeled with a fluorescent or ... Labeling of annexin A5 with fluorescent or radioactive molecules makes it possible to detect binding of labeled annexin A5 to ... Internalization amplifies additionally the intensity of the annexin A5 stained cell. Annexin A5 has been used to successively ... annexin A5 assembles into a trimeric cluster. This trimer consists of three annexin A5 molecules that are bound to each other ...
Takano T, Fiore S, Maddox JF, Brady HR, Petasis NA, Serhan CN (May 1997). "Aspirin-triggered 15-epi-lipoxin A4 (LXA4) and LXA4 ... Ernst S, Lange C, Wilbers A, Goebeler V, Gerke V, Rescher U (Jun 2004). "An annexin 1 N-terminal peptide activates leukocytes ... "The synthetic peptide Trp-Lys-Tyr-Met-Val-Met-NH2 specifically activates neutrophils through FPRL1/lipoxin A4 receptors and is ... is not affected by lipoxin A4". Scandinavian Journal of Immunology. 56 (5): 470-6. doi:10.1046/j.1365-3083.2002.01149.x. PMID ...
... which may be reflected by increased expression of the membrane fusion proteins NAPA and Annexin A3.[20] ...
2012). "The S100A10 subunit of the annexin A2 heterotetramer facilitates L2-mediated human papillomavirus infection". PLOS ONE ... E1, E2, E4, E5, E6, and E7) open reading frames (ORF), two late (L1 and L2) ORFs, and a non-coding long control region (LCR). ... and annexin A2 leading to entry of the virions into basal epithelial cells through clathrin-mediated endocytosis and/or ...
... lipoxin A4 (LXA4). Because of its interaction with lipoxin A4, FPR2 is also commonly named the ALX/FPR2 or just ALX receptor. ... Ac2-26 and Ac9-25 of Annexin A1 (ANXA1 or lipocortin 1), which at high concentrations fully stimulate neutrophil functions but ... lipoxin A4 (LXA4), and thereafter for a related arachidonic acid metabolite, the Epi-lipoxin, aspirin-triggered lipoxin A4 (i.e ... "Lipoxin A4 stable analogs are potent mimetics that stimulate human monocytes and THP-1 cells via a G-protein-linked lipoxin A4 ...
018229 ANXA6 Annexin 6 ANXA7 Annexin 7 AP1B1 Coated vesicles CLTA Clathrin A (vesicles) CLTB Clathrin B (vesicles) CLTC MTX2 ... 021178 E3 ubiquitin-protein ligase. Modulates cyclin B levels and participates in the regulation of cell cycle progression ... 003334 Homo sapiens ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature UBA2 NM_005499 UBA3 NM_003968 UBA5 NM_024818 ...
These products are now termed lipoxin A4 and B4, respectively. While initially found to have in vitro activity suggesting that ... annexin A1 (an inhibitor of formation of pro-inflammatory metabolites of polyunsaturated fatty acids), and the gaseous ...
Annexin *A1. *A2. *A5. *Fibulin *FBLN1. *FBLN2. *FBLN5. *Vitamin D-dependent calcium-binding protein/Calbindin ...
2002). „Phagocyte activation by Trp-Lys-Tyr-Met-Val-Met, acting through FPRL1/LXA4R, is not affected by lipoxin A4.". Scand. J ... 2004). „An annexin 1 N-terminal peptide activates leukocytes by triggering different members of the formyl peptide receptor ... 2001). „The synthetic peptide Trp-Lys-Tyr-Met-Val-Met-NH2 specifically activates neutrophils through FPRL1/lipoxin A4 receptors ...
Genome organization of human papillomavirus type 16, one of the subtypes known to cause cervical cancer (E1-E7 early genes, L1- ... and annexin A2[93] leading to entry of the virions into basal epithelial cells through clathrin-mediated endocytosis and/or ... E1, E2, E4, E5, E6, and E7) open-reading frames (ORF), two late (L1 and L2) ORFs, and a non-coding long control region (LCR).[ ... "The S100A10 subunit of the annexin A2 heterotetramer facilitates L2-mediated human papillomavirus infection". PLoS One. 7 (8 ...
Chemical structures of major endogenous estrogens, including estrone (E1), estradiol (E2), estriol (E3), and estetrol (E4).[9] ... "Activation of the annexin A1 pathway underlies the protective effects exerted by estrogen in polymorphonuclear leukocytes" ... Note the hydroxyl (-OH) groups: estrone (E1) has one, estradiol (E2) has two, estriol (E3) has three, and estetrol (E4) has ... The three major naturally occurring forms of estrogen in females are estrone (E1), estradiol (E2), and estriol (E3). Another ...
This clustering of annexin A5 on the membrane greatly increases the intensity of annexin A5 when labeled with a fluorescent or ... Labeling of annexin A5 with fluorescent or radioactive molecules makes it possible to detect binding of labeled annexin A5 to ... Internalization amplifies additionally the intensity of the annexin A5 stained cell. Annexin A5 has been used to successively ... annexin A5 assembles into a trimeric cluster. This trimer consists of three annexin A5 molecules that are bound to each other ...
Compare Anti-Annexin A5 Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, reviews ... Anti-Annexin A5 Antibody Products. Anti-Annexin A5 antibodies are available from several suppliers. In humans, this protein is ... Antibodies to the Annexin A5 antigen may be useful for studying apoptosis. Visit the supplier page for more detailed antibody ... Annexin V is an early apoptotic marker and is often used for detecting apoptosis in cells. Annexins are a family of calcium- ...
Rabbit polyclonal Annexin A3/ANXA3 antibody. Validated in WB and tested in Human. Immunogen corresponding to recombinant ... Anti-Annexin A3/ANXA3 antibody. See all Annexin A3/ANXA3 primary antibodies. ... This product Rabbit Anti-Annexin A3/ANXA3 antibody (ab228761) WB Goat Anti-Rabbit IgG H&L (HRP) (ab205718) IHC-P, WB, ELISA, IP ... All lanes : Anti-Annexin A3/ANXA3 antibody (ab228761) at 1/1000 dilution. Lane 1 : A431 (human epidermoid carcinoma cell line) ...
... annexin A3) Alliance Pan paniscus (bonobo/pygmy chimpanzee) : ANXA3 (annexin A3) Canis lupus familiaris (dog) : ANXA3 (annexin ... ANXA3 (annexin A3). NCBI. Ortholog. Mus musculus (house mouse):. Anxa3 (annexin A3). Transitive Ortholog Pipeline. Transitive ... Anxa3 (annexin A3). Transitive Ortholog Pipeline. Transitive Ortholog Pipeline. Sus scrofa (pig):. ANXA3 (annexin A3). ... Homo sapiens (human) : ANXA3 (annexin A3) HGNC Alliance Mus musculus (house mouse) : Anxa3 (annexin A3) MGI Alliance Rattus ...
The crystal structure of annexin A8 is similar to that of annexin A3. Réty, S., Sopková-de Oliveira Santos, J., Dreyfuss, L., ... The crystal structure of annexin A8 is similar to that of annexin A3 [17]. ... Annexin A3 is a potential angiogenic mediator. Park, J.E., Lee, d.o. .H., Lee, J.A., Park, S.G., Kim, N.S., Park, B.C., Cho, S ... The high-resolution crystal structure of human annexin III shows subtle differences with annexin V. Favier-Perron, B., Lewit- ...
Mouse Monoclonal Anti-Annexin A3 Antibody (2F3). Validated: WB, ICC/IF, IHC, IHC-P. Tested Reactivity: Human, Monkey. 100% ... Blogs on Annexin A3. There are no specific blogs for Annexin A3, but you can read our latest blog posts. ... Reviews for Annexin A3 Antibody (NBP2-03373) (0) There are no reviews for Annexin A3 Antibody (NBP2-03373). By submitting a ... Diseases for Annexin A3 Antibody (NBP2-03373). Discover more about diseases related to Annexin A3 Antibody (NBP2-03373). ...
ANX4, ANXA4, Annexin-IV, Lipocortin-4, Lipocortin-IV, Annexin-4, Annexin A4, Endonexin I, Chromobindin-4, Protein II, P32.5, ... ANXA4 is part of the annexin family of calcium-dependent phospholipid binding proteins. ANXA4 has 45%-59% identity with other ...
Annexin A5, ENX2, Placental Anticoagulant Protein 4, Placental Anticoagulant Protein I, Vascular Anticoagulant-Alpha, ... Thromboplastin Inhibitor, Calphobindin I, Anchorin CII, Endonexin II, Lipocortin V, Annexin V, Annexin-5, VAC-Alpha, RPRGL3, ... ANXA5 is a member of the annexin family of calcium-dependent phospholipid binding proteins which are involved in membrane- ...
A protein of the annexin family isolated from human PLACENTA and other tissues. It inhibits cytosolic PHOSPHOLIPASE A2, and ... Annexin A5, human. diannexin, human. technetium 99m tricarbonyl His-annexin A5. technetium Tc 99m HYNIC annexin V ... annexin A5. Known as: Calphobindin I, Annexin V, Lipocortin-V (More). A protein of the annexin family isolated from human ... Annexin A5 polymorphism (-1C--,T) and the presence of anti-annexin A5 antibodies in the antiphospholipid syndrome. ...
Annexin A5 Protein Recombinant is available 3 times from supplier abbex at Gentaur.com shop ... Annexin A5 Protein Recombinant. Annexin A5 Protein Recombinant is available 3 times from Abbex labs abx260555 , Annexin A5 ... Annexin A4 Protein Recombinant Suppplier: abbex. Price: 295.25 USD Annexin A3 Protein Recombinant Suppplier: abbex. Price: ... abx260555 , Annexin A5 Protein (Recombinant) size: 5 µg , 295.25 USD Catalog number abx260555 ...
"Annexin A3" by people in this website by year, and whether "Annexin A3" was a major or minor topic of these publications. ... "Annexin A3" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... Below are the most recent publications written about "Annexin A3" by people in Profiles. ... Below are MeSH descriptors whose meaning is more general than "Annexin A3". ...
Annexin A5 positron emission tomography (PET) reveals the externalization of phosphatidylserine as a surrogate marker for ... 68Ga]Annexin A5 uptake was 7.4 ± 1.3 %ID/g within the infarction for the controls and 4.5 ± 1.9 %ID/g for the PTH group (p = . ... 68Ga]Annexin A5 PET can detect the effects of PTH treatment as a marker of apoptosis 2 days after MI; ex vivo examination ... Abstract[68Ga]Annexin A5 positron emission tomography (PET) reveals the externalization of phosphatidylserine as a surrogate ...
Annexin A5 Binding to LDL. We observed a strong binding of annexin A5 to the captured oxidized LDL but not to captured native ... It is not known whether annexin A5 binds to LDL and/or interferes with binding of CRP to LDL. Association of annexin A5 with ... Moreover, we show that annexin A5 binds to a site at oxidized LDL different from the CRP binding site and that annexin A5 does ... Enzyme-Linked Immunosorbent Assay for CRP and Annexin A5 Binding to Antigens. Binding of CRP and annexin A5 to LDL was ...
Annexin A5 (ANXA5) has been linked with a variety of cellular functions including: endocytosis, Ca2+ transfer, anticoagulation ... Annexin A5, ANXA5, Ca2+ channel, membrane interaction, membrane binding, PS, Phosphatidylserine, channel formation, structural ... Stewart, David John Ossian (2019) Investigating the metamorphic properties of annexin A5 during membrane integration. PhD ... The annexin superfamily is comprised of a range of Ca2+ dependent phospholipid binding proteins. Each of these proteins are ...
This gene encodes a member of the annexin family.Members of this calcium-dependent phospholipid-binding protein family play a ... Recombinant Human Annexin A3, His-tagged. E. coli. Human. His. +Inquiry. ANXA3-1834H. Recombinant Human Annexin A3. E. coli. ... Annexin III; Annexin-3; AnnexinA3; AnnexinIII; ANX 3; ANX A3; ANX3; ANXA 3; ANXA3_HUMAN; Calcimedin 35 alpha; Inositol 1 2 ... Hamelin-Peyron, C; Vlaeminck-Guillem, V; et al. Prostate cancer biomarker annexin A3 detected in urines obtained following ...
Annexin 5 is a;phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in; ... Annexin 5 has also been described as placental;anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin ... The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some;of which ... Recombinant Human Annexin A5, APC. E. coli. Human. N/A. +Inquiry. ANXA5-2624H. Recombinant Human Annexin A5, Biotin. E. coli. ...
Annexin A3 antibody LS-C172488 is an unconjugated mouse monoclonal antibody to human Annexin A3 (ANXA3). Validated for IHC and ... ANXA3 , 35-alpha calcimedin , Annexin A3 , ANX3 , Annexin-3 , Annexin III , Calcimedin 35-alpha , Lipocortin III , Annexin III ... Annexin A3 antibody LS-C172488 is an unconjugated mouse monoclonal antibody to human Annexin A3 (ANXA3). Validated for IHC and ... Monoclonal Mouse anti‑Human ANXA3 / Annexin A3 Antibody (clone OTI5G4, IHC, WB) LS‑C172488 ...
Depletion of annexin A5, annexin A6 and collagen X causes no gross changes in matrix vesicle mediated mineralization. Pubmed ID ... Depletion of annexin A5, annexin A6 and collagen X causes no gross changes in matrix vesicle mediated mineralization. Journal ...
Effect of Hydroxychloroquine on the Annexin A5 Resistance Assay in Antiphospholipid Antibody-Positive Patients with and without ... on a blood test called the annexin A5 (AnxA5) resistance assay in people with antiphospholipid antibodies (aPL). People with ...
Human annexin A5 is a ubiquitous protein implicated in diverse signal transduction processes associated with cell growth and ... Promoter transcriptional activity was determined for a wide 5′ portion of the human annexin A5 gene, from bp −1275 to +79 ... Functional analysis of the human annexin A5 gene promoter: a downstream DNA element and an upstream long terminal repeat ... Functional analysis of the human annexin A5 gene promoter: a downstream DNA element and an upstream long terminal repeat ...
The apoptosis-detecting radiotracer Tc-99m-(CO)(3) His-annexin A5 was injected (18.5 MBq) in mice 12, 24, and 48 h after the ... The apoptosis-detecting radiotracer Tc-99m-(CO)(3) His-annexin A5 was injected (18.5 MBq) in mice 12, 24, and 48 h after the ... SPECT analyses demonstrated a significant increase in tumoral Tc-99m-(CO)(3) His-annexin A5 uptake 4 d after bevacizumab ... SPECT analyses demonstrated a significant increase in tumoral Tc-99m-(CO)(3) His-annexin A5 uptake 4 d after bevacizumab ...
Annexin A4 (AnxA4) has been implicated in diverse cellular processes, including the regulation of exocytosis and ion-transport ... Quantitative analysis of self-association and mobility of annexin a4 at the plasma membrane. ... Annexins, found in most eukaryotic species, are cytosolic proteins that are able to bind negatively-charged phospholipids in a ...
Description Annexin A5 Polyclonal Antibody. Unconjugated. Raised in Rabbit. Formulation Liquid. 0.03% Proclin 300,... ... Home » Antibodies » Primary Antibodies » Cell Structure & Function Antibodies » Apoptosis & Cell Death Antibodies » Annexin A5 ... Recombinant Cynops pyrrhogaster Annexin A5 protein (1-323AA). Storage. Shipped at 4°C. Upon delivery aliquot and store at -20°C ... Annexin A5 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.. Formulation. Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M ...
Annexin A5 forms a shield around negatively charged phospholipid molecules, thus reducing their availability for coagulation. ... Thus, anti-annexin A5 antibodies increase phospholipid-dependent coagulation steps. The lupus anticoagulant antibodies are ...
Annexin A5 has been more widely used to target PS exposure. Hiller and co-workers [54] recently reported on a new Anx A5-based ... This molecular imaging protocol is based upon the protein annexin A5 (Anx A5), which binds with high selectivity and with great ... Annexin A5. In the past decade, a molecular imaging protocol was established to detect and measure apoptosis in vitro and in ... The annexin A5 imaging protocol is emphasised since it is the most advanced protocol to measure apoptosis in both preclinical ...
title = "Annexin A5 binds to lipopolysaccharide and reduces its endotoxin activity",. abstract = "Annexin A5 (AnxA5) has a high ... Annexin A5 binds to lipopolysaccharide and reduces its endotoxin activity. Jacob H. Rand, Xiao Xuan Wu, Elaine Y. Lin, ... Annexin A5 binds to lipopolysaccharide and reduces its endotoxin activity. / Rand, Jacob H.; Wu, Xiao Xuan; Lin, Elaine Y.; ... Rand, J. H., Wu, X. X., Lin, E. Y., Griffel, A., Gialanella, P., & McKitrick, J. C. (2012). Annexin A5 binds to ...
Annexin A3. Alternative Names. ANX-A3; ANX3; PAP-III; 35-alpha calcimedin; Inositol 1,2-cyclic phosphate 2-phosphohydrolase; ... Recombinant Annexin A3 (ANXA3), Cat#RPU40302. Write a Review Write a Review. × ... Recombinant Annexin A3 (ANXA3), Cat#RPU40302. Rating Required Select Rating. 1 star (worst). 2 stars. 3 stars (average). 4 ...
The phospholipid- and Ca2+-binding protein annexin A5 (ANXA5) is the most abundant membrane-associated protein of ~P23 mouse ... Annexin A5 is the Most-Abundant Membrane-Associated Protein in Stereocilia but is Dispensable ... GILL185_ANXA5_Ca2vsNoCa2_bundle_TGT2_DATA_Annexins_2016-03-18_16-08-41.sky.zip. (2 MB). 2016-03-23. 6. 27. 30. 167. 8. ... Annexins have been proposed to mediate Ca2+-dependent repair of membrane lesions, which could be part of the repair mechanism ...
Annexin A5, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene ... ANXA5 (Annexin A5) is a Protein Coding gene. Diseases associated with ANXA5 include Pregnancy Loss, Recurrent 3 and Pregnancy ... A common haplotype of the annexin A5 (ANXA5) gene promoter is associated with recurrent pregnancy loss. (PMID: 17339269) ... annexin A5,calcium and phospholipid binding protein,endonexin 2,placental protein 4,anchorin CII collagen receptor of ...
Annexin A4,Annexin IV,Annexin-4,ANX4,ANXA4,Chromobindin-4,Endonexin I,Lipocortin IV,P32.5,PAP-II,Pig,Placental anticoagulant ... ELISA kit 35-beta calcimedin,Annexin A4,Annexin IV,Annexin-4,ANX4,ANXA4,Chromobindin-4,Endonexin I,Lipocortin IV,P32.5,PAP-II, ... ELISA kit 35-beta calcimedin,Annexin A4,Annexin IV,Annexin-4,ANX4,ANXA4,Chromobindin-4,Endonexin I,Lipocortin IV,P32.5,PAP-II, ... Product name : ELISA kit 35-beta calcimedin,Annexin A4,Annexin IV,Annexin-4,ANX4,ANXA4,Chromobindin-4,Endonexin I,Lipocortin IV ...
  • Recombinant fragment within Human Annexin A3/ANXA3 (internal sequence). (abcam.com)
  • Annexin A3 antibody LS-C172488 is an unconjugated mouse monoclonal antibody to human Annexin A3 (ANXA3). (lsbio.com)
  • Our previous study identified an elevated abundance of annexin A3 (Anxa3) as a novel prognostic biomarker of lung adenocarcinoma (LADC) through quantitative proteomics analysis. (cancerindex.org)
  • Flow cytometric analysis of mock (blue) and ANXA3-transfected (red) HEK293T cells using anti-Annexin A3 mouse monoclonal antibody. (genetex.com)
  • Anti-Annexin A5 antibodies are available from several suppliers. (biocompare.com)
  • Antibodies to the Annexin A5 antigen may be useful for studying apoptosis. (biocompare.com)
  • Your search returned 479 Annexin A5 Antibodies across 40 suppliers. (biocompare.com)
  • T) and the presence of anti-annexin A5 antibodies in the antiphospholipid syndrome. (semanticscholar.org)
  • This effect was independent of the presence of complement and could be inhibited by irrelevant IgG and by antibodies to CD64 but not by annexin A5. (ahajournals.org)
  • The purpose of this study is to determine the effects of a medication, hydroxychloroquine (HCQ), on a blood test called the annexin A5 (AnxA5) resistance assay in people with antiphospholipid antibodies (aPL). (hss.edu)
  • Thus, anti-annexin A5 antibodies increase phospholipid-dependent coagulation steps. (wikipedia.org)
  • For the detection of anti-annexin A5 antibodies and the measurement of annexin A5 plasma levels, ELISA-type methods were used. (cdc.gov)
  • 71 patients were positive for annexin A5 IgM or IgG antibodies, of whom 53 patients were positive for anti-annexin A5 IgG antibodies and 27 of 198 patients were positive for anti-annexin A5 IgM antibodies. (cdc.gov)
  • The prevalence of IgM or IgG anti-annexin A5 antibodies was not significantly associated with thrombosis or miscarriage on multivariate analysis. (cdc.gov)
  • The detection of anti-annexin A5 antibodies does not seem relevant for estimating the risk for thrombosis or miscarriage in APS. (cdc.gov)
  • Plasma annexin A5, anti-annexin A5 antibodies and annexin A5 polymorphism in Egyptian female patients with systemic lupus erythematosus and antipho. (cdc.gov)
  • The present study aimed to find out a possible relationship between annexin A5 (gene polymorphism, antibodies or plasma level) and the pathophysiology of SLE, APS and RPL. (cdc.gov)
  • Anti-annexin A5 antibodies (IgG and IgM) and annexin A5 plasma level were measured by Enzyme Linked Immunosorbent Assay (ELISA). (cdc.gov)
  • The primary interest of our laboratory is the investigation of the effects of antiphospholipid (aPL) antibodies on an anticoagulant protein, annexin A5 (previously known as annexin V). The antiphospholipid (aPL) syndrome is an enigmatic autoimmune disorder that is characterized by thrombosis and/or recurrent spontaneous pregnancy losses that occur in patients who have evidence for antibodies against phospholipid-binding proteins. (yu.edu)
  • We were the first to propose and demonstrate that aPL antibodies disrupt the annexin A5 anticoagulant shield on artificial bilayers and on cell membranes. (yu.edu)
  • Western Blot: Annexin A3 Antibody (2F3) [NBP2-03373] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Annexin A3 (Right lane) cDNA for 48 hrs and lysed. (novusbio.com)
  • Immunocytochemistry/ Immunofluorescence: Annexin A3 Antibody (2F3) [NBP2-03373] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Annexin A3. (novusbio.com)
  • Immunohistochemistry-Paraffin: Annexin A3 Antibody (2F3) [NBP2-03373] - Staining of paraffin-embedded Human prostate tissue using anti-Annexin A3 mouse monoclonal antibody. (novusbio.com)
  • The efficacy of antihuman VEGF antibody (bevacizumab) with or without irinotecan was evaluated in a colorectal cancer xenograft using Tc-99m-(CO)(3) His-annexin A5. (ugent.be)
  • Analytical sensitivity of the anti‐annexin A5 antibody ELISA. (cdc.gov)
  • Anti-annexin A5 IgM was the antibody associated with RPL in this group of APS patients. (cdc.gov)
  • Western blot analysis in extracts from 9 cell lines using anti-Annexin 3 mouse monoclonal antibody. (genetex.com)
  • Paraffin-embedded mouse cornea is stained with Annexin V Antibody used at 1:200 dilution. (antibodies-online.com)
  • WB analysis of various samples using GTX30048 Annexin IV antibody.The signal was developed with ECL plus-Enhanced. (genetex.com)
  • Antibody-mediated interference with annexins in the antiphospholipid syndrome. (yu.edu)
  • Detection of antibody-mediated reduction of annexin A5 anticoagulant activity in plasmas of patients with the antiphospholipid syndrome. (yu.edu)
  • In molecular biology, an annexin A5 affinity assay is a test to quantify the number of cells undergoing apoptosis. (wikipedia.org)
  • Abstract[68Ga]Annexin A5 positron emission tomography (PET) reveals the externalization of phosphatidylserine as a surrogate marker for apoptosis. (biomedsearch.com)
  • The apoptosis-detecting radiotracer Tc-99m-(CO)(3) His-annexin A5 was injected (18.5 MBq) in mice 12, 24, and 48 h after the start of the irinotecan or NaCl treatment, and micro-SPECT was subsequently performed 3.5 h after injection of the radiotracer. (ugent.be)
  • The annexin A5 imaging protocol is emphasised since it is the most advanced protocol to measure apoptosis in both preclinical and clinical studies. (springer.com)
  • Objectives: The purpose of this study was to evaluate the role of caspase inhibitors on acute resolution of apoptosis in atherosclerotic lesions as evaluated by imaging with annexin A5. (fujita-hu.ac.jp)
  • Conclusions: Molecular imaging, using radiolabeled annexin A5, allows the detection of acute resolution of apoptosis as a result of caspase inhibition in experimental atherosclerosis. (fujita-hu.ac.jp)
  • Reutelingsperger, Chris P.M. / Engineered annexin A5 variants have impaired cell entry for molecular imaging of apoptosis using pretargeting strategies . (utmb.edu)
  • Therefore the measurement of Annexin V binding to the cell surface as indicative for apoptosis has to be performed in conjunction with a dye exclusion test to establish integrity of the cell membrane. (nih.gov)
  • The Annexin V assay offers the possibility of detecting early phases of apoptosis before the loss of cell membrane integrity and permits measurements of the kinetics of apoptotic death in relation to the cell cycle. (nih.gov)
  • Annexin V-PE-Cy5 Apoptosis Staining / Detection Kit (ab14159) is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. (abcam.com)
  • The protein may also be known as Annexin V, ANX5, ENX2, HEL-S-7, PP4, CBP-I, and PAP-I. The reported amino acid length is 320 and the expected mass is 35.9 kDa. (biocompare.com)
  • ANXA4 is part of the annexin family of calcium-dependent phospholipid binding proteins. (prospecbio.com)
  • ANXA5 is a member of the annexin family of calcium-dependent phospholipid binding proteins which are involved in membrane-related activity along exocytotic and endocytotic pathways. (prospecbio.com)
  • Objective- To investigate binding of C-reactive protein (CRP) and annexin A5, 2 proteins with high affinity for negatively charged phospholipids, to oxidized low-density lipoprotein (LDL) and the consequences of these interactions for subsequent binding of oxidized LDL to monocyte/macrophage-like U937 cells. (ahajournals.org)
  • The annexin superfamily is comprised of a range of Ca2+ dependent phospholipid binding proteins. (gla.ac.uk)
  • The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some;of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. (creativebiomart.net)
  • Annexins, found in most eukaryotic species, are cytosolic proteins that are able to bind negatively-charged phospholipids in a calcium-dependent manner. (ru.nl)
  • Combined the experimental data in the urine and in tumor tissues collected from patients with UTUC, the crucial over-expressed proteins are calreticulin, annexin A2, and annexin A3. (beds.ac.uk)
  • Human Annexin A13 (ANXA13), also known as Annexin XIII or Annexin 13, is a 35 kDa member of the Annexin family (1), which are calcium-dependent phospholipid-binding proteins being preferentially located on the cytosolic face of the plasma membrane. (novusbio.com)
  • Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. (biolegend.com)
  • Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. (genetex.com)
  • By polynomial first‐order equation, an average R 2 of 0.9929 was calculated for the anti‐annexin A5 IgG ELISA, and 0.9926 for the anti‐annexin A5 IgM ELISA. (cdc.gov)
  • ANX‐A5 levels were measured with "a sandwich‐ELISA‐type" assay. (cdc.gov)
  • The annexin a5 protein binds to apoptotic cells in a calcium-dependent manner using phosphatidylserine-containing membrane surfaces that are usually present only on the inner leaflet of the membrane. (wikipedia.org)
  • Annexin A5 uptake in ischemic myocardium: demonstration of reversible phosphatidylserine externalization and feasibility of radionuclide imaging. (semanticscholar.org)
  • Lactadherin binding and phosphatidylserine expression on cell surface-comparison with annexin A5. (semanticscholar.org)
  • Annexin A5 (AnxA5) has a high affinity for phosphatidylserine. (elsevier.com)
  • Phosphatidylserine (PS) on apoptotic cells is a target for diagnosis and therapy using annexin A5 (anxA5). (utmb.edu)
  • Flow cytometry analyses of inflammatory cells obtained from four naturally cysticercotic pigs, and stained with Annexin-V/PI, showed lymphocytes expressing phosphatidylserine with values of 0, 6, 41 and 56% on their outer surfaces. (biomedsearch.com)
  • Western blot analysis of Annexin A3 expression in mock (left lane) and AXNA3-transfected (right lane) HEK293T cells. (genetex.com)
  • The assay uses the protein annexin A5 to tag apoptotic and dead cells, and the numbers are then counted using either flow cytometry or a fluorescence microscope. (wikipedia.org)
  • Detection of annexin A5 (-1C/T) gene polymorphism was done by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) assay. (cdc.gov)
  • In comparison with existing traditional tests the Annexin V assay is sensitive and easy to perform. (nih.gov)
  • Annexin A5 is an intracellular protein that is abundantly present in endothelial cells and platelets, and which exhibits a high affinity for anionic phospholipids in membranes. (ahajournals.org)
  • 19 Because of its high affinity for anionic phospholipids, similar to CRP, annexin A5 might have an inhibitory effect on macrophage uptake of oxidized LDL via scavenger receptors. (ahajournals.org)
  • Annexin A5 (A5) is a phospholipid-binding protein with potent anticoagulant properties that are a consequence of its forming 2-dimensional crystals over membranes containing anionic phospholipids. (yu.edu)
  • Full length human recombinant protein of human Annexin III (NP_005130) produced in HEK293T cells. (genetex.com)
  • 68Ga]Annexin A5 uptake was 7.4 ± 1.3 %ID/g within the infarction for the controls and 4.5 ± 1.9 %ID/g for the PTH group (p = .013). (biomedsearch.com)
  • Here, we evaluated the aortic uptake of 18F-FDG and 99mTc-annexin A5 in apolipoprotein E-deficient (apoE2/2) and wild-type mice placed on high-fat diets. (hokudai.ac.jp)
  • Aortic tracer uptake [(ID%/g) · kg] remained stable with the regular diet in wild-type mice (0.054- 0.053 and 0.021-0.023 for 99mTc-annexin A5) but increased with cholesterol feeding in wild-type mice (0.164 for 18F-FDG and 0.036 for 99mTc-annexin A5 at 25 wk) and in apoE2/2 mice (0.249 for 18F-FDG and 0.047 for 99mTc-annexin A5 at 25 wk). (hokudai.ac.jp)
  • The phospholipid- and Ca2+-binding protein annexin A5 (ANXA5) is the most abundant membrane-associated protein of ~P23 mouse vestibular hair bundles, the inner ear's sensory organelle. (panoramaweb.org)
  • This gene encodes a member of the annexin family. (novusbio.com)
  • This gene encodes a member of the annexin family.Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways.This protein functions in the inhibition of phopholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. (creativebiomart.net)
  • ANXA10: a member of the annexin family. (mybiosource.com)
  • Members of the Annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways (3, 4). (novusbio.com)
  • Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. (genetex.com)
  • Dying cells in tissue can be detected with annexin A5. (wikipedia.org)
  • Labeling of annexin A5 with fluorescent or radioactive molecules makes it possible to detect binding of labeled annexin A5 to the cell surface of apoptotic cells. (wikipedia.org)
  • Two-dimensional crystal formation is believed to cause internalization of annexin A5 through a novel process of endocytosis if it occurs on cells that are in the early phase of executing cell death. (wikipedia.org)
  • Annexin A5 has been used to successively detect apoptotic cells in vitro and in vivo. (wikipedia.org)
  • Annexin A5 alone had no effect on binding of oxidized LDL to the cells. (ahajournals.org)
  • and (3) annexin A5 does not antagonize the CRP-induced enhanced binding of oxidized LDL to U937 cells. (ahajournals.org)
  • Annexins have been proposed to mediate Ca2+-dependent repair of membrane lesions, which could be part of the repair mechanism in hair cells after noise damage. (panoramaweb.org)
  • Detection of A5 single-positive cells during necroptosis. (nih.gov)
  • (C) Example single A5-FITC-positive necroptotic cells, imaged using live microscopy. (nih.gov)
  • (G-H) 3 x 10 4 HaCaT cells per well in 96-well plate were stimulated, and PI and A5-FITC were added. (nih.gov)
  • Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. (biolegend.com)
  • Annexin V binding alone cannot differentiate between apoptotic cells and necrotic. (biolegend.com)
  • For flow cytometric staining, the suggested use of this reagent is 5 µl per 100,000 - million cells in a 100 µl volume of Annexin V Binding Buffer (Cat No. 422201). (biolegend.com)
  • No. 420201) and then resuspend cells in Annexin V Binding Buffer (Cat. (biolegend.com)
  • no. 422201) is recommended for use with Annexin V staining.Annexin V binding alone cannot differentiate between apoptotic cells and necrotic. (biolegend.com)
  • 1. Wash cells twice with cold BioLegend cell staining buffer (cat # 420201) and then resuspend cells in Annexin V Binding Buffer (cat # 422201) at a concentration of 1x10e6 cells/ml. (biolegend.com)
  • A5 is highly expressed by cells with barrier functions, including endothelial cells and placental trophoblasts. (yu.edu)
  • Annexin A5 is a lipid-binding protein produced by cells. (bioquicknews.com)
  • Annexin V binding was assessed using bivariate FCM, and cell staining was evaluated with fluorescein isothiocyanate (FITC)-labelled Annexin V (green fluorescence), simultaneously with dye exclusion of propidium iodide (PI) (negative for red fluorescence). (nih.gov)
  • The pro-resolution mediators Annexin-A1 AnxA1 and lipoxin A4 LXA4 exert counter-regulatory effects on leukocyte recruitment, however to date, the dual-cumulative effects of these formyl peptide receptor-2 FPR2-ALX agonists in the context of human intestinal diseases are unclear. (duhnnae.com)
  • The frequency of annexin A5 (-1C/T) polymorphism was significantly higher in SLE related groups (p = 0.02), but it did not correlate with RPL (p = 0.57) or annexin A5 level (p = 0.5). (cdc.gov)
  • Annexin A5 plasma levels are not affected by the presence of annexin A5 (-1C/T) polymorphism. (cdc.gov)
  • Annexin 5 has also been described as placental;anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin;CII. (creativebiomart.net)
  • Although seven other annexin genes are expressed in mouse utricles, shotgun and targeted mass spectrometry showed that none were present at levels near ANXA5 in bundles and none were upregulated in stereocilia of Anxa5-/- mice. (panoramaweb.org)
  • The non-invasive detection of diseased tissue with, for example, radioactively labeled annexin A5 is the goal of a recently developed line of research known as Molecular Imaging. (wikipedia.org)
  • Annexin V is a Ca2+ dependent phospholipid-binding protein with high affinity for PS. (nih.gov)
  • Annexin A5 exhibits anticoagulant properties that appear to be defective in patients with antiphospholipid syndrome (APS) resulting in repeated thrombosis and recurrent pregnancy loss (RPL). (cdc.gov)
  • A common haplotype of the annexin A5 (ANXA5) gene promoter is associated with recurrent pregnancy loss. (semanticscholar.org)
  • On day 2, they received [68Ga]annexin A5 PET or histofluorescence TUNEL staining. (biomedsearch.com)
  • En face staining was then performed to assess the location and size (surface area) of the lipid pool within each aortic specimen. (hokudai.ac.jp)
  • Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. (abcam.com)
  • (B) Cell viability was measured at different time points after stimulation of cell death using A5/PI staining and then analyzed by flow cytometry (mean ± SD). (nih.gov)
  • No. 422201) is recommended for use with Annexin V staining. (biolegend.com)
  • Annexin V staining in mouse cornea. (antibodies-online.com)
  • Molecular Imaging of cell death using radioactive annexin A5 can become of clinical significance to diagnose vulnerability of atherosclerotic plaques (unstable atherosclerosis), heart failure, transplant rejection, and to monitor efficacy of anti-cancer therapy. (wikipedia.org)
  • This clustering of annexin A5 on the membrane greatly increases the intensity of annexin A5 when labeled with a fluorescent or radioactive probe. (wikipedia.org)
  • The annexin A5-mediated pathogenic mechanism in the antiphospholipid syndrome: role in pregnancy losses and thrombosis. (semanticscholar.org)
  • Annexin A5 is thought to have a role in the pathophysiology of the antiphospholipid syndrome (APS)-a syndrome characterised by recurrent thrombosis and pregnancy morbidity. (cdc.gov)
  • Discovery of the "Disruption of the Annexin A5 Anticoagulant Crystal Shield" concept as a mechanism of thrombosis and pregnancy loss in the antiphospholipid syndrome. (yu.edu)
  • Also known as Annexin A1 (Annexin I) (Annexin-1) (Calpactin II) (Calpactin-2) (Chromobindin-9) (Lipocortin I) (Lipocortin-like 33 kDa protein) (Phospholipase A2 inhibitory protein) (p35). (mybiosource.com)
  • Description This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. (gentaur.com)
  • Annexin A3" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)