Annexin A2: A member of the annexin family that is a substrate for a tyrosine kinase, ONCOGENE PROTEIN PP60(V-SRC). Annexin A2 occurs as a 36-KDa monomer and in a 90-KDa complex containing two subunits of annexin A2 and two subunits of S100 FAMILY PROTEIN P11. The monomeric form of annexin A2 was formerly referred to as calpactin I heavy chain.Annexin A1: Protein of the annexin family exhibiting lipid interaction and steroid-inducibility.Annexin A5: A protein of the annexin family isolated from human PLACENTA and other tissues. It inhibits cytosolic PHOSPHOLIPASE A2, and displays anticoagulant activity.Annexin A6: Protein of the annexin family with a probable role in exocytotic and endocytotic membrane events.Annexin A4: Protein of the annexin family originally isolated from the electric organ of the electric ray Torpedo marmorata. It has been found in a wide range of mammalian tissue where it is localized to the apical membrane of polarized EPITHELIAL CELLS.Annexin A7: An annexin family member that plays a role in MEMBRANE FUSION and signaling via VOLTAGE-DEPENDENT CALCIUM CHANNELS.Annexin A3: A protein of the annexin family that catalyzes the conversion of 1-D-inositol 1,2-cyclic phosphate and water to 1-D-myo-inositol 1-phosphate.Annexins: Family of calcium- and phospholipid-binding proteins which are structurally related and exhibit immunological cross-reactivity. Each member contains four homologous 70-kDa repeats. The annexins are differentially distributed in vertebrate tissues (and lower eukaryotes) and appear to be involved in MEMBRANE FUSION and SIGNAL TRANSDUCTION.S100 Proteins: A family of highly acidic calcium-binding proteins found in large concentration in the brain and believed to be glial in origin. They are also found in other organs in the body. They have in common the EF-hand motif (EF HAND MOTIFS) found on a number of calcium binding proteins. The name of this family derives from the property of being soluble in a 100% saturated ammonium sulfate solution.Phosphatidylserines: Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a serine moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and serine and 2 moles of fatty acids.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.Propidium: Quaternary ammonium analog of ethidium; an intercalating dye with a specific affinity to certain forms of DNA and, used as diiodide, to separate them in density gradients; also forms fluorescent complexes with cholinesterase which it inhibits.Calcium: A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.Phospholipids: Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.Calcium-Binding Proteins: Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Cell Line, Tumor: A cell line derived from cultured tumor cells.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Flow Cytometry: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Cell-Derived Microparticles: Extracellular vesicles generated by the shedding of CELL MEMBRANE blebs.Receptors, Formyl Peptide: A family of G-protein-coupled receptors that was originally identified by its ability to bind N-formyl peptides such as N-FORMYLMETHIONINE LEUCYL-PHENYLALANINE. Since N-formyl peptides are found in MITOCHONDRIA and BACTERIA, this class of receptors is believed to play a role in mediating cellular responses to cellular damage and bacterial invasion. However, non-formylated peptide ligands have also been found for this receptor class.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Caspase 3: A short pro-domain caspase that plays an effector role in APOPTOSIS. It is activated by INITIATOR CASPASES such as CASPASE 9. Isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.Organotechnetium Compounds: Organic compounds that contain technetium as an integral part of the molecule. These compounds are often used as radionuclide imaging agents.Cell Survival: The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Caspases: A family of intracellular CYSTEINE ENDOPEPTIDASES that play a role in regulating INFLAMMATION and APOPTOSIS. They specifically cleave peptides at a CYSTEINE amino acid that follows an ASPARTIC ACID residue. Caspases are activated by proteolytic cleavage of a precursor form to yield large and small subunits that form the enzyme. Since the cleavage site within precursors matches the specificity of caspases, sequential activation of precursors by activated caspases can occur.Fluorescein-5-isothiocyanate: Fluorescent probe capable of being conjugated to tissue and proteins. It is used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.In Situ Nick-End Labeling: An in situ method for detecting areas of DNA which are nicked during APOPTOSIS. Terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 prime OH ends of either single- or double-stranded DNA. The terminal deoxynucleotidyl transferase nick end labeling, or TUNEL, assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA FRAGMENTATION.Fibrinolysin: A product of the lysis of plasminogen (profibrinolysin) by PLASMINOGEN activators. It is composed of two polypeptide chains, light (B) and heavy (A), with a molecular weight of 75,000. It is the major proteolytic enzyme involved in blood clot retraction or the lysis of fibrin and quickly inactivated by antiplasmins.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.

The Npc1 mutation causes an altered expression of caveolin-1, annexin II and protein kinases and phosphorylation of caveolin-1 and annexin II in murine livers. (1/431)

We have previously demonstrated (1) an increased expression of caveolin-1 in murine heterozygous and homozygous Niemann-Pick type C (NPC) livers, and (2) an increased concentration of unesterified cholesterol in a detergent insoluble caveolae-enriched fraction from homozygous livers. To define further the relationship between caveolin-1 function and the cholesterol trafficking defect in NPC, we examined the expression and distribution of additional caveolar and signal transduction proteins. The expression of annexin II was significantly increased in homozygous liver homogenates and the Triton X-100 insoluble floating fraction (TIFF). Phosphoamino acid analysis of caveolin-1 and annexin II from the homozygous TIFF demonstrated an increase in serine and tyrosine phosphorylation, respectively. To determine the basis for increased phosphorylation of these proteins, the expression and distribution of several protein kinases was examined. The expression of PKCalpha, PKCzeta and pp60-src (protein kinases) were significantly increased in both heterozygous and homozygous liver homogenates, while PKCdelta was increased only in homozygous livers. Of the protein kinases analyzed, only CK IIalpha was significantly enriched in the heterozygous TIFF. Finally, the concentration of diacylglycerol in the homozygous TIFF was significantly increased and this elevation may modulate PKC distribution and function. These results provide additional evidence for involvement of a caveolin-1 containing cellular fraction in the pathophysiology of NPC and also suggest that the Npc1 gene product may directly or indirectly, regulate the expression and distribution of signaling molecules.  (+info)

Tissue plasminogen activator and its receptor in the human amnion, chorion, and decidua at preterm and term. (2/431)

The plasminogen activator system consists of two proteins: tissue plasminogen activator (tPA) and urokinase plasminogen activator (uPA), which act upon their specific receptors to generate plasmin from plasminogen located on the cell surface. Plasmin then acts directly and indirectly to degrade the components of the extracellular matrix (ECM). This process is likely to be important in the normal turnover of the ECM of fetal membranes and in its premature weakening in preterm premature rupture of the fetal membranes. Quantitative Northern analysis and in situ hybridization have shown that the decidua expresses mRNA for tPA. However, the immunolocalized tPA protein was most strongly associated with the amnion and chorion, as was its receptor annexin II, suggesting that the amnion and chorion are the targets for decidual tPA. At term, decidual tPA expression was unaffected by labor, and the tPA receptor was elevated both before and after labor. At preterm, the converse was found: decidual tPA expression was significantly (p < 0. 05) up-regulated by labor, but the tPA receptor was not. The results suggest that the generation of plasmin at term would be controlled by an increased concentration of the tPA receptor in the amnion and chorion, whereas at preterm a pathological increase in plasmin would be generated by an overexpression of tPA, initiated by labor.  (+info)

Annexin II and bleeding in acute promyelocytic leukemia. (3/431)

BACKGROUND: Acute promyelocytic leukemia (APL) is associated with a hemorrhagic disorder of unknown cause that responds to treatment with all-trans-retinoic acid. METHODS: We studied a newly described receptor for fibrinolytic proteins, annexin II, in cells from patients with APL or other leukemias. We examined initial rates of in vitro generation of plasmin by tissue plasminogen activator (t-PA) in the presence of APL cells that did or did not have the characteristic translocation of APL, t(15;17). We also determined the effect of all-trans-retinoic acid on the expression of annexin II and the generation of cell-surface plasmin. RESULTS: The expression of annexin II, as detected by a fluorescein-tagged antibody, was greater on leukemic cells from patients with APL than on other types of leukemic cells (mean fluorescence intensity, 6.9 and 2.9, respectively; P<0.01). The t(15;17)-positive APL cells stimulated the generation of cell-surface, t-PA-dependent plasmin twice as efficiently as the t(15;17)-negative cells. This increase in plasmin was blocked by an anti-annexin II antibody and was induced by transfection of t(15;17)-negative cells with annexin II complementary DNA. The t(15;17)-positive APL cells contained abundant messenger RNA for annexin II, which disappeared through a transcriptional mechanism after treatment with all-trans-retinoic acid. CONCLUSIONS: Abnormally high levels of expression of annexin II on APL cells increase the production of plasmin, a fibrinolytic protein. Overexpression of annexin II may be a mechanism for the hemorrhagic complications of APL.  (+info)

Localization and quantitation of cardiac annexins II, V, and VI in hypertensive guinea pigs. (4/431)

Annexins are characterized by Ca2+-dependent binding to phospholipids. Annexin II mainly participates in cell-cell adhesion and signal transduction, whereas annexins V and VI also seem to regulate intracellular calcium cycling. Their abundance and localization were determined in left ventricle (LV) and right ventricle (RV) from hypertensive guinea pigs, during the transition from compensatory hypertrophy to heart failure. Immunoblot analysis of annexins II, V, and VI revealed an increased accumulation (2.6-, 1.45-, and 2.3-fold, respectively) in LV from hypertensive guinea pigs and no modification in RV. Immunofluorescent labeling of annexins II, V, and VI; of Na+-K+-ATPase; and of sarcomeric alpha-actinin showed that in control LV and RV, 1) annexin II is present in nonmuscle cells; 2) annexins V and VI are mainly observed in the sarcolemma and intercalated disks of myocytes; 3) annexins II, V, and VI strongly label endothelial cells and adventitia of coronary arteries; and 4) annexin VI is present in the media. At the onset of heart failure, the most striking changes are the increased protein accumulation in LV and the very strong labeling of annexins II, V, and VI in interstitial tissue, suggesting a role in fibrosis development and cardiac remodeling.  (+info)

The biological effects induced in mice by p36, a proteinaceous factor of virulence produced by African swine fever virus, are mediated by interleukin-4 and also to a lesser extent by interleukin-10. (5/431)

We have previously presented indirect evidence that both specific immunosuppression and lymphocyte mitogenicity induced in mice by p36, a proteinaceous factor of virulence produced by porcine monocytes infected by African swine fever virus, were consistent with a Th2-driven response. Here we show: (1) Interleukin-4 (IL-4) and interleukin-10 (IL-10) mRNA expression in the spleen and thymus of C57BL/6 mice were displayed early after p36 inoculation. The expression of thymic IL-10 mRNA occurred, however, later than that of IL-4 mRNA. (2) Increased serum levels of these two cytokines were also soon detected after the protein inoculation. (3) Both immunosuppressive and mitogenic effects of p36 were absent in IL-4 gene-targeted mice and partially abrogated in mice depleted of IL-4 by neutralizing monoclonal antibodies. (4) IL-10 depletion abrogated the immunosuppressive but not the p36 lymphocyte mitogenic biological effects. (5) The increase in the serum concentrations of both IL-4 and IL-10 were lower in thymectomized than in non-thymectomized mice. (6) The expression of interferon-gamma (IFN-gamma) mRNA was weakly or not at all induced in p36-treated mice. Taken together, these results are in agreement with the promotion of a Th2 immune response induced by p36.  (+info)

Dexamethasone alters arachidonate release from human epithelial cells by induction of p11 protein synthesis and inhibition of phospholipase A2 activity. (6/431)

The effect of the glucocorticosteroid, dexamethasone, on arachidonic acid (AA) release and on protein levels of p11 and cytosolic phospholipase A2 (cPLA2) was studied in two epithelial cell lines, HeLa cells and BEAS-2B cells. Dexamethasone treatment of HeLa cells and BEAS-2B cells increased cellular p11 protein and mRNA levels in a time- and dose-dependent manner. It had little effect on levels of cPLA2 protein. In order to determine if increased p11 protein expression resulted in increased interaction between p11 and cPLA2, anti-cPLA2 antibodies were used to immunoprecipitate p11.cPLA2 complexes and Western blots of the immunoprecipitate were used to detect p11. In cells treated with dexamethasone, more p11 was detected in the anti-cPLA2 immunoprecipitate compared with control cells. Dexamethasone treatment of HeLa cells prelabeled with [3H]AA decreased the release of [3H]AA under basal conditions and after stimulation with the calcium ionophore A23187 (10(-6) M). In order to determine if altering the p11 protein levels in HeLa cells independent of glucocorticosteroid treatment could also produce an effect on [3H]AA release, cells were stably transfected with plasmids expressing either p11 antisense mRNA or p11 mRNA. Cloned HeLa cells expressing p11 antisense mRNA exhibited less cellular p11 protein compared with control cells and greater [3H]AA release compared with cells transfected with a control vector. Cloned HeLa cells stably transfected with a p11 expression vector exhibited increased p11 cellular protein and diminished [3H]AA release under basal conditions and in response to A23187. Therefore, dexamethasone alteration of epithelial cell AA release may be due in part to induction of p11 protein expression.  (+info)

Annexin II increases osteoclast formation by stimulating the proliferation of osteoclast precursors in human marrow cultures. (7/431)

Annexin II (AXII), a calcium-dependent phospholipid-binding protein, has been recently found to be an osteoclast (OCL) stimulatory factor that is also secreted by OCLs. In vitro studies showed that AXII induced OCL formation and bone resorption. However, the mechanism of action by which AXII acts as a soluble extracellular protein to induce OCL formation is unknown. In this paper, we demonstrate that AXII gene expression is upregulated by 1,25-dihydroxyvitamin D3 [1, 25-(OH)2D3] and that addition of AXII significantly increased OCL-like multinucleated cell formation. Time-course studies suggested that AXII acted on the proliferative stage of OCL precursors and that AXII increased thymidine incorporation in OCL precursors. Moreover, AXII enhanced the growth of CFU-GM, the earliest identifiable OCL precursor, when bone marrow cultures were treated with low concentrations of GM-CSF. This capacity of AXII to induce OCL precursor proliferation was due to induction of GM-CSF expression, because the addition of neutralizing antibodies to GM-CSF blocked the stimulatory effect of AXII on OCL formation. RT-PCR analysis using RNA from highly purified subpopulations of marrow cells demonstrated that T cells, especially CD4(+) T cells, produced GM-CSF in response to AXII. Furthermore, FACS(R) analysis of T-cell subpopulations treated with fluorescein-labeled AXII suggested that the CD4(+), but not CD8(+), subpopulation of T cells express an AXII receptor. Taken together, these data suggest that AXII stimulates OCL formation by activating T cells through a putative receptor to secrete GM-CSF. GM-CSF then expands the OCL precursor pool to enhance OCL formation.  (+info)

Interference with annexin II has no effect on entry of human cytomegalovirus into fibroblast cells. (8/431)

Annexin II has been identified as a human cytomegalovirus (HCMV)-binding protein, shown to be a component of purified virions and proposed as a cellular receptor for the virus. In addition, annexin II is capable of associating with the major HCMV envelope glycoprotein, gB (gpUL55). As one approach to examine the role of annexin II in virus entry, a high-titre polyclonal annexin II-specific antibody was produced and its effects in virus entry and cell-to-cell spread assays were tested. This anti-annexin II serum recognized virion and cell surface annexin II and annexin II-derived peptides. Recombinant annexin II, with characteristic calcium- and phospholipid-binding activities, was also examined. Pretreatment of cells, virions or both with polyclonal anti-annexin II serum, affinity-purified anti-annexin II antibodies or recombinant annexin II protein prior to infection was inconsequential to the entry of HCMV into fibroblasts. HCMV was also able to dose-dependently penetrate annexin II-deficient 293 cells. Furthermore, the spread of HCMV from cell to cell was not inhibited in the presence of polyclonal anti-annexin II antibodies or exogenous annexin II protein. These experiments do not support a direct role of annexin II in virus entry or spread. Alternative roles for the gB-annexin II interaction are proposed.  (+info)

*Annexin A1

... protein has an apparent relative molecular mass of 40 kDa with phospholipase A2 inhibitory activity. Glucocorticoids ... Annexin A1, also known as lipocortin I, is a protein that is encoded by the ANXA1 gene in humans. Annexin A1 belongs to the ... Annexin A1 both suppresses phospholipase A2, thereby blocking eicosanoid production, and inhibits various leukocyte ... Annexin A1 has also been shown to be associated with treatment resistance. ARID1A loss activates annexin A1 expression, which ...

*Annexin A2

... also known as annexin II is a protein that in humans is encoded by the ANXA2 gene. Annexin 2 is involved in diverse ... Kwon M, MacLeod TJ, Zhang Y, Waisman DM (Jan 2005). "S100A10, annexin A2, and annexin a2 heterotetramer as candidate ... "Entrez Gene: ANXA2 annexin A2". Kling T, Ferrarese R, Ó hAilín D, Johansson P, Heiland DH, Dai F, Vasilikos I, Weyerbrock A, ... Annexin A2 has been proposed to function inside the cell in sorting of endosomes and outside the cell in anticoagulant ...

*CEACAM1

... has been shown to interact with PTPN11 and Annexin A2. Carcinoembryonic antigen Cluster of differentiation GRCh38: ... Kirshner J, Schumann D, Shively JE (December 2003). "CEACAM1, a cell-cell adhesion molecule, directly associates with annexin ...

*PCSK9

Annexin A2, an endogenous protein, is a natural inhibitor of PCSK9 activity. GRCh38: Ensembl release 89: ENSG00000169174 - ... Mayer G, Poirier S, Seidah NG (November 2008). "Annexin A2 is a C-terminal PCSK9-binding protein that regulates endogenous low ... "Annexin A2 is a natural extrahepatic inhibitor of the PCSK9-induced LDL receptor degradation". PLoS ONE. 7 (7): e41865. doi: ...

*Ubiquitin C

He KL, Deora AB, Xiong H, Ling Q, Weksler BB, Niesvizky R, Hajjar KA (July 2008). "Endothelial cell annexin A2 regulates ...

*Human papillomavirus infection

... and annexin A2 leading to entry of the virions into basal epithelial cells through clathrin-mediated endocytosis and/or ... "The S100A10 subunit of the annexin A2 heterotetramer facilitates L2-mediated human papillomavirus infection". PLoS One. 7 (8): ...

*SLPI

Additionally, it has been shown that SLPI is able to bind the Annexin A2/S100A10 heterotetramer (A2t), a co-factor HIV ... "The S100A10 subunit of the annexin A2 heterotetramer facilitates L2-mediated human papillomavirus infection". PLOS ONE. 7 (8): ... "Secretory leukocyte protease inhibitor binds to annexin II, a cofactor for macrophage HIV-1 infection". The Journal of ...

*S100A10

Kwon M, MacLeod TJ, Zhang Y, Waisman DM (Jan 2005). "S100A10, annexin A2, and annexin a2 heterotetramer as candidate ... He KL, Deora AB, Xiong H, Ling Q, Weksler BB, Niesvizky R, Hajjar KA (Jul 2008). "Endothelial cell annexin A2 regulates ... Annexin II, which is attracted to negatively charged phospholipids, binds to p11 at the Ca2+ binding site. In addition, Annexin ... The p11 and annexin II complex is regulated by the phosphorylation of SerII on the annexin II molecule by protein kinase C (PKC ...

*RAB3D

Knop M, Aareskjold E, Bode G, Gerke V (2005). "Rab3D and annexin A2 play a role in regulated secretion of vWF, but not tPA, ...

*Prohibitin

"Prohibitin and prohibitone are contained in high-molecular weight complexes and interact with alpha-actinin and annexin A2". ...

*Langerhans cell

Specifically, HPV16 entry into Langerhans cells via the annexin A2/S100A10 heterotetramer results in suppressive signaling and ... a novel role for the annexin A2 heterotetramer in immune suppression". Journal of Immunology. 192 (10): 4748-4757. doi:10.4049/ ...

*Annexin

However some annexins (Annexin A1, Annexin A2, and Annexin A5) have also been found outside the cellular environment, for ... Annexin, type I InterPro: IPR002388 Annexin, type II InterPro: IPR002389 Annexin, type III InterPro: IPR002390 Annexin, type IV ... In addition to annexin A-II, annexin A-XI has also been shown to organize cell membrane properties. Annexin A-XI is believed to ... The 310 amino acid annexin core has four annexin repeats, each composed of 5 alpha-helices. The exception is annexin A-VI that ...

*Embryo quality

Two examples of proteins that may be included in maternal profiling are endometrium-derived stathmin 1 and annexin A2, whose ...

*List of MeSH codes (D12.776.157)

... annexin a1 MeSH D12.776.157.125.050.060 -- annexin a2 MeSH D12.776.157.125.050.070 -- Annexin A3 MeSH D12.776.157.125.050.080 ... annexin a4 MeSH D12.776.157.125.050.100 -- annexin a5 MeSH D12.776.157.125.050.110 -- annexin a6 MeSH D12.776.157.125.050.120 ... annexin a7 MeSH D12.776.157.125.412.249 -- calmodulin MeSH D12.776.157.125.412.311 -- calnexin MeSH D12.776.157.125.412.374 -- ...

*Proliferating cell nuclear antigen

Annexin A2, CAF-1, CDC25C, CHTF18, Cyclin D1, Cyclin O, Cyclin-dependent kinase 4, Cyclin-dependent kinase inhibitor 1C, DNMT1 ...

*A2

... a protein that in humans is encoded by the EDA2R gene Alpha-2 adrenergic receptor Annexin A2, a protein Apolipoprotein A2, a ... A2, A02, A002, A², A.II or A-2 may refer to: A2 Racer, a racing computer game series A2 - Secret of the Slavers Stockade, a ... Anadol A2, a 1970 Turkish car Arrows A2, a 1979 British racing car Audi A2, a 1999 automobile Audi Quattro A2, a 1984 rally car ... noncombustible A2, an Italian Baseball League HEBA A2, a Greek basketball league A2, an Italian Volleyball League A2 volleyball ...

*Dysferlin

Lennon NJ, Kho A, Bacskai BJ, Perlmutter SL, Hyman BT, Brown RH (2003). "Dysferlin interacts with annexins A1 and A2 and ... including annexin and MG53. Exactly how dysferlin contributes to membrane resealing is not clear, but biochemical evidence ...

*Alclometasone

... induces the production of lipocortins, formally known as annexins, which inhibit phospholipase A2 - the enzyme ...

*Catgrip

... s are found as calcium-binding features in annexins, matrix metalloproteinases (e.g.serralysins), subtilisins and ... phospholipase A2. They are also observed in synthetic peptides and in cyclic hexapeptides made from alternating D,L amino acids ...

*Annexin A5

"Sedimentation equilibrium analysis of five lipocortin-related phospholipase A2 inhibitors from human placenta. Evidence against ... Annexin A5 (or annexin V) is a cellular protein in the annexin group. In flow cytometry, annexin V is commonly used to detect ... The annexin A5 affinity assay typically uses a conjugate of annexin V and a fluorescent or enzymatic label, biotin or other ... Annexin A5 showed upregulation in papillary thyroid carcinoma. Annexin A5 is used as a non-quantitative probe to detect cells ...

*Annexin A3

This protein functions in the inhibition of phospholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1- ... "The high-resolution crystal structure of human annexin III shows subtle differences with annexin V". Biochemistry. 35 (6): 1740 ... Annexin A3 is a protein that in humans is encoded by the ANXA3 gene. It is abnormally expressed in fetuses of both IVF and ICSI ... "Entrez Gene: ANXA3 annexin A3". Zhang Y, Zhang YL, Feng C, et al. (September 2008). "Comparative proteomic analysis of human ...

*Glucocorticoid

... annexin-1) synthesis. Lipocortin-1 both suppresses phospholipase A2, thereby blocking eicosanoid production, and inhibits ... They inhibit prostaglandin synthesis at the level of phospholipase A2 as well as at the level of cyclooxygenase/PGE isomerase ( ... "Glucocorticoids inhibit prostaglandin synthesis not only at the level of phospholipase A2 but also at the level of cyclo- ...

*Immunosuppressive drug

They induce the lipocortin-1 (annexin-1) synthesis, which then binds to cell membranes preventing the phospholipase A2 from ...

*Annexin A4

... and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in ... Annexin A4 is a protein that in humans is encoded by the ANXA4 gene. Annexin IV (ANX4) belongs to the annexin family of calcium ... "Entrez Gene: ANXA4 annexin A4". Human ANXA4 genome location and ANXA4 gene details page in the UCSC Genome Browser. Römisch J, ... Dreier R, Schmid KW, Gerke V, Riehemann K (Aug 1998). "Differential expression of annexins I, II and IV in human tissues: an ...

*ANXA8L2

Annexin A8-like protein 2 is a protein that in humans is encoded by the ANXA8L2 gene. This gene encodes a member of the annexin ... a novel human Ca2+/phospholipid binding protein that inhibits coagulation and phospholipase A2 activity. Its molecular cloning ... 2006). "Annexin A8 is up-regulated during mouse mammary gland involution and predicts poor survival in breast cancer". Clin. ... Gerke V, Creutz CE, Moss SE (2005). "Annexins: linking Ca2+ signalling to membrane dynamics". Nat. Rev. Mol. Cell Biol. 6 (6): ...

*Selective glucocorticoid receptor modulator

Examples of glucocorticoid-responsive genes include those that encode annexin A1, TSC22D3 (also known as GILZ), angiotensin- ... These target genes encode proteins such as cyclooxygenase, NO synthase, phospholipase A2, tumor necrosis factor, transforming ...
C57BL/6 mice were immunized intraperitoneally twice with 100 nmol of I-Ab-restricted MOG35-55 peptide (MEVGWYRSPFSRVVHLYRNGK, MBL code no. TS-M704-P) and 10 µg cholera toxin (MBL code no. RK-01-511) in complete Freunds adjuvant. Eleven days later, splenocytes were isolated and stained with I-Ab MOG35-55 tetramer-PE (MBL code no. TS-M704-1; Day 0). An aliquot of splenocytes was stimulated with 10µg/mL MOG35-55 peptide for 6 days in vitro and then stained with MHC class II tetramer (Day 6). in vitro stimulation of with I-Ab MOG35-55 resulted in increased tetramer-positive CD4+ T cells from immunized (mouse 1 and 2), but not naïve (mouse 3), mice.. ...
Annexin II antibody (annexin A2) for ICC/IF, IHC-P, IP, RipA, WB. Anti-Annexin II pAb (GTX32446) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
Annexin A2 is a calcium-dependent, phospholipid-binding protein found on many cell types. It consists of a short hydrophobic tail (Ser2-Asn32), which dictates its function, and a core domain (Phe33-Asp339), which is involved in phospholipid binding. Annexin A2 has been implicated in a number of biochemical processes, including cell proliferation, foetal immune tolerance, ion-channel activation, cell-cell interactions and the bridging of membranes. Annexin A2 is reported to be a powerful activator of plasminogen and, therefore, is implicated in many normal and pathological processes such as haemostasis and metastasis. Myeloid cell lines are used, extensively, to study many aspects of cellular proliferation, differentiation and function. In the present study, we have used flow cytometry and real-time PCR to investigate the role of annexin A2 expression in the proliferation and differentiation of a number of myeloid cell lines. The results demonstrated that annexin A2 expression was affected when ...
Purpose: : To examine the role of annexin 2 in the formation and internalisation of phagosomes in retinal pigment epithelial (RPE) cells. Methods: : Human ARPE19 cells were cultured on the glass inserts of Matek dishes for immunofluorescence and live imaging, or plastic Nunc dishes for biochemical analysis. In some studies ARPE19 cells were exposed to siRNA for annexin 2 for three days prior to experimentation. Outer segments were prepared from porcine retina using standard protocols (typically 50 - 100 eyes per prep), and either used fresh in biochemical experiments, or labelled with an appropriate fluorescent marker prior to imaging experiments. Results: : Depletion of annexin 2 using siRNA led to a significant quantitative reduction in the phagocytosis of rod outer segments in cultured RPE cells. In cells expressing annexin 2, imaged by confocal microscopy, a striking enrichment of annexin 2 and F-actin was observed at the phagocytic cup immediately upon engagement of the apical cell surface ...
In our hands, the trypsin actually reduced the Annexin staining presumably by stripping off all the phosphotidyl-serine on the surface along with the surface proteins (adhesion molecules). The only time we have seen enhanced annexin is when the cells suffer enough trauma in harvesting that the membrane is not intact and the Annexin gets inside the cell and attaches to the ps on the inner membrane. Julie At 02:11 PM 8/18/98, you wrote: , ,I have a client who is staining with Annexin-FITC. They are looking at ,cell lines and are using trypsin to get the cells off the flasks. We are ,seeing what we think is an exagerated Annexin response. Does anyone out ,there have any feedback on how trypsin affects Annexin staining? Any ,experience and or information about this will be greatly appreciated. , Thanks in advance, , Joan Kalnitsky ,Joan Kalnitsky ,Flow Cytometry Laboratory ,VMRCVM ,(540) 231-4115 ,FAX 540-231-7367 , , It is better to serve than to receive , B. Borg , , , , , ...
Low Ag dose promotes induction and persistence of regulatory T cells (Tregs) in mice, yet few studies have addressed the role of Ag dose in the induction of adaptive CD4+FOXP3+ Tregs in humans. To this end, we examined the level of FOXP3 expression in human CD4+CD25− T cells upon activation with autologous APCs and varying doses of peptide. Ag-specific T cells expressing FOXP3 were identified by flow cytometry using MHC class II tetramer (Tmr). We found an inverse relationship between Ag dose and the frequency of FOXP3+ cells for both foreign Ag-specific and self Ag-specific T cells. Through studies of FOXP3 locus demethylation and helios expression, we determined that variation in the frequency of Tmr+FOXP3+ T cells was not due to expansion of natural Tregs, but instead, we found that induction, proliferation, and persistence of FOXP3+ cells was similar in high- and low-dose cultures, whereas proliferation of FOXP3− T cells was favored in high Ag dose cultures. The frequency of FOXP3+ cells ...
The Annexin family of calcium-binding proteins is composed of al least thirteen mammalian genes (Annexin A1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. The Annexin family
Annexin A1 antibody (annexin A1) for ICC/IF, IHC-P, IP, WB. Anti-Annexin A1 pAb (GTX101070) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
Order monoclonal and polyclonal Annexin A13 antibodies for many applications. Selected quality suppliers for anti-Annexin A13 antibodies.
Annexin A1兔多克隆抗体(ab65844)可与小鼠, 大鼠, 人样本反应并经WB, IP, IHC, ICC实验严格验证。所有产品均提供质保服务,中国75%以上现货。
Annexin A2兔多克隆抗体(ab90643)可与人样本反应并经WB, IHC实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
Abcam provides specific protocols for Anti-Annexin A10 antibody (ab2343) : Western blot protocols, Immunohistochemistry protocols
BioAssay record AID 590043 submitted by ChEMBL: Induction of apoptosis in human K562 cells assessed as viable cells at 50 nM using annexin V-propidium iodide and annexin V staining by flow cytometry (Rvb = 87.02%).
Annexin A5 is a biomarker used in Hiv Infection, Acute Lymphoblastic Leukemia, Hypertension and 942 other diseases. Learn more about Annexin A5.
Rabbit recombinant monoclonal Annexin A10 antibody [EPR13101] validated for WB and tested in Human, Mouse and Rat. Immunogen corresponding to recombinant…
BioAssay record AID 502910 submitted by ChEMBL: Induction of annexin 2-mediated cytoskeleton aggregate accumulation in human HepG2 cells at 4 uM after 2 hrs by confocal laser scanning microscopy.
Annexin A11 Antibody 10479-2-AP has been identified with IF, IHC, IP, WB, ELISA. 10479-2-AP detected 56-60 kDa band in HeLa cells with 1:500-1:2000 dilution...
Annexin A1 Antibody 66344-1-Ig has been identified with ELISA, IF, IHC, WB. 66344-1-Ig detected 35 kDa band in A431 cells with 1:2500-1:10000 dilution...
Bachem offers H-3196 Annexin A1 (1-25) (dephosphorylated) (human) for your research. Find all specific details here. Find product specific information including available pack sizes, CAS, detailed description and references here.
Mouse Monoclonal Anti-Annexin A3 Antibody (2F3). Validated: WB, ICC/IF, IHC, IHC-P. Tested Reactivity: Human, Monkey. 100% Guaranteed.
In epithelial cells, AHNAK is predominantly a cytosolic protein localized at the plasma membrane intracellular face. A faint AHNAK immunoreactivity within the cell nuclei was observed in cells submitted to a Ca2+ switch (Fig. 5 C), but was never observed in other cell culture conditions. The apparent exclusive cytoplasmic localization of AHNAK in MDCK cells contrasts with the results of Shtivelmans group, who reported nuclear localization of AHNAK in sparse MDCK cell cultures (Sussman et al., 2001). Several possible explanations could be envisioned to explain the apparent discrepancies in subcellular AHNAK localization in MDCK cells. Some discrepancies can arise from the well-known heterogeneities in specific cell lines or clones in the various laboratories. Differences in epitope accessibility might also have to be considered. AHNAK nuclear immunoreactivity has only been reported with one antibody, FEN (Shtivelman and Bishop, 1993; Sussman et al., 2001). Because a recent work has pointed out ...
Sigma-Aldrich offers abstracts and full-text articles by [Jiangnan Li, Dongwei Guo, Li Huang, Manman Yin, Qingfang Liu, Yan Wang, Chunmei Yang, Yuanyuan Liu, Lijie Zhang, Zhijun Tian, Xuehui Cai, Liyun Yu, Changjiang Weng].
Annexin A1 is an intracellular calcium/phospholipid-binding protein that is involved in membrane organization and the regulation of the immune system. It has been attributed an anti-inflammatory role at various control levels, and recently we could show that annexin A1 externalization during secondary necrosis provides an important fail-safe mechanism counteracting inflammatory responses when the timely clearance of apoptotic cells has failed. As such, annexin A1 promotes the engulfment of dying cells and dampens the postphagocytic production of proinflammatory cytokines. In our current follow-up study, we report that exposure of annexin A1 during secondary necrosis coincided with proteolytic processing within its unique N-terminal domain by ADAM10. Most importantly, we demonstrate that the released peptide and culture supernatants of secondary necrotic, annexin A1-externalizing cells induced chemoattraction of monocytes, which was clearly reduced in annexin A1- or ADAM10-knockdown cells. Thus, ...
The anti-inflammatory protein annexin 1 may protect patients from the ...Severe inflammatory response syndrome or SIRS occurs when the bodys...Damazo et al. studied this balancing act by analyzing the effects of t...When normal mice were treated with LPS the immune system induced a st...The researchers characterized the expression of annexin 1 and found th...,Protein,prevents,detrimental,immune,effects,of,bacterial,sepsis,biological,biology news articles,biology news today,latest biology news,current biology news,biology newsletters
Membrane repair is essential to cell survival. In skeletal muscle, injury often associates with plasma membrane disruption. Additionally, muscular dystrophy is linked to mutations in genes that produce fragile membranes or reduce membrane repair. Methods to enhance repair and reduce susceptibility to injury could benefit muscle in both acute and chronic injury settings. Annexins are a family of membrane-associated Ca2+-binding proteins implicated in repair, and annexin A6 was previously identified as a genetic modifier of muscle injury and disease. Annexin A6 forms the repair cap over the site of membrane disruption. To elucidate how annexins facilitate repair, we visualized annexin cap formation during injury. We found that annexin cap size positively correlated with increasing Ca2+ concentrations. We also found that annexin overexpression promoted external blebs enriched in Ca2+ and correlated with a reduction of intracellular Ca2+ at the injury site. Annexin A6 overexpression reduced membrane ...
The pHi (intracellular pH) is an important physiological parameter which is altered during hypoxia and ischaemia, pathological conditions accompanied by a dramatic decrease in pHi. Sensors of pHi include ion transport systems which control intracellular Ca2+ gradients and link changes in pHi to functions as diverse as proliferation and apoptosis. The annexins are a protein family characterized by Ca2+-dependent interactions with cellular membranes. Additionally, in vitro evidence points to the existence of pH-dependent, Ca2+-independent membrane association of several annexins. We show that hypoxia promotes the interaction of the recombinant annexin A2-S100A10 (p11) and annexin A6 with the plasma membrane. We have investigated in vivo the influence of the pHi on the membrane association of human annexins A1, A2, A4, A5 and A6 tagged with fluorescent proteins, and characterized this interaction for endogenous annexins present in smooth muscle and HEK (human embryonic kidney)-293 cells ...
|p|Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry
ELISA method for the measurement of IgM isotype auto antibodies targeted to annexin V. Assay designed with highly purified human recombinant annexin V, obtained with non-denaturing methods, for auto antibody immunocapture. These are then revealed with a goat antibody specific for IgM, micro fragments, and labeled with peroxidase.
In the present study, we show that both CRP and annexin A5 specifically bind to oxidized LDL. Via coupling to Fcγ receptors on macrophages and independent of complement, CRP subsequently enhances the association of oxidized LDL to macrophages. Enhanced association of oxidized LDL to macrophages via CRP-Fcγ receptor interaction may lead to enhanced macrophage uptake of oxidized LDL. Moreover, we show that annexin A5 binds to a site at oxidized LDL different from the CRP binding site and that annexin A5 does not antagonize the CRP effect.. Previously, Chang et al also observed specific binding of CRP to oxidized LDL,7 characterized by inhibition by whole molecules as well as F(ab′)2 fragments of EO6, an IgM antibody that binds specifically to the phosphorylcholine head group of oxidized phospholipids but not to the same moiety on nonoxidized phospholipids.26 In the present study, for characterization of binding sites, we used the monoclonal antibody DLH3 (also IgM), which recognizes an epitope ...
TransDetect® Annexin V-EGFP/PI Cell Apoptosis Detection Kit,Cell Detection,Cell Culture and Detection,Products,Beijing TransGen Biotech Co.Ltd,OverviewContents& storageCitations & referencesRelated ImagesDownloadOverviewDescriptionThe Annexin V
Animals. Wild-type mice from the 129T2/SvEmsJ background were bred and housed in a specific pathogen-free facility on a 12-hour light/12-hour dark cycle and fed ad libitum in accordance with the Northwestern Universitys Institutional Animal Care and Use Committee regulations. 129T2/SvEmsJ (129T2) mice were purchased from the Jackson Laboratory (stock 002065). mdx/hLTBP4 mice were generated as described previously (34, 60). Sgcg-null mice were generated as described in Hack et al. (46). Two- to 3-month-old males and females were used for all wild-type mouse experiments. Sgcg-null cohorts were age and sex matched with mice between 2 and 5 months old.. Plasmids. Plasmids encoding annexin A1, A2, and A6 with a carboxyl-terminal turboGFP tag were obtained from Origene. Subcloning of annexin A1, A2, and A6 to replace the GFP tag with tdTomato (Addgene) was performed by Mutagenix. Site-directed mutagenesis was performed by Mutagenix on annexin A1-GFP, A2-GFP, and A6-GFP to create the Ca2+-binding ...
Animals. Wild-type mice from the 129T2/SvEmsJ background were bred and housed in a specific pathogen-free facility on a 12-hour light/12-hour dark cycle and fed ad libitum in accordance with the Northwestern Universitys Institutional Animal Care and Use Committee regulations. 129T2/SvEmsJ (129T2) mice were purchased from the Jackson Laboratory (stock 002065). mdx/hLTBP4 mice were generated as described previously (34, 60). Sgcg-null mice were generated as described in Hack et al. (46). Two- to 3-month-old males and females were used for all wild-type mouse experiments. Sgcg-null cohorts were age and sex matched with mice between 2 and 5 months old.. Plasmids. Plasmids encoding annexin A1, A2, and A6 with a carboxyl-terminal turboGFP tag were obtained from Origene. Subcloning of annexin A1, A2, and A6 to replace the GFP tag with tdTomato (Addgene) was performed by Mutagenix. Site-directed mutagenesis was performed by Mutagenix on annexin A1-GFP, A2-GFP, and A6-GFP to create the Ca2+-binding ...
With Annexin V Apoptosis Assay, detect one of the earliest events in apoptosis-the externalization of phosphatidylserine (PS)-in living cells
Annexin V is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Annexin V is a monomer, binds ATRX and EIF5B, and interacts with hepatitis B virus (HBV ...
SCAN HISTORY OWL11_0 2 100 NSINGLE OWL17_1 2 110 NSINGLE OWL18_0 1 115 NSINGLE OWL19_1 1 125 NSINGLE OWL21_1 1 125 NSINGLE OWL26_0 1 125 NSINGLE SPTR37_9f 2 237 NSINGLE INITIAL MOTIF SETS ANNEXIN1 Length of motif = 23 Motif number = 1 Annexin motif I - 1 PCODE ST INT KTKGVDEVTIVNILTNRSNAQRQ LUHU36 46 46 KTKGVDEVTIINILTNRSNEQRQ ANX2_CHICK 46 46 TVKGVDEATIIDILTKRNNAQRQ ANX1_CAVCU 56 56 MVKGVDEATIIDILTKRNNAQRQ LUHU 55 55 KGIGTDEATIIDIVTHRSNAQRQ ANX6_MOUSE 376 376 KGMGTDEETILKILTSRNNAQRQ ANX5_CHICK 29 29 KGIGTNEQAIIDVLTKRSNTQRQ ANX8_HUMAN 35 35 KGFGTDEQEIIDVLVGRSNQQRQ DROANNX 29 29 RGIGTDEKMLISILTERSNAQRQ ANX3_HUMAN 32 32 KGLGTDEDAIINVLAYRSTAQRQ ANX4_BOVIN 27 27 KGFGTDEQAIVDVVANRSNDQRQ HUMSNEXIN 177 177 TAKGVDEATIIDIMTTRTNAQRP ANX1_COLLI 51 51 ANNEXIN2 Length of motif = 17 Motif number = 2 Annexin motif II - 1 PCODE ST INT LKSALSGHLETVILGLL LUHU36 86 17 LKSALSGHLEAVILGLL ANX2_CHICK 86 17 LKKALTGHLEEVVLALL ANX1_CAVCU 96 17 LKKALTGHLEEVVLALL LUHU 95 17 LKSEISGDLARLILGLM ANX6_MOUSE 416 17 ...
Buy ANXA2 recombinant protein, Annexin A2 Recombinant Protein-NP_001002857 (MBS203687) product datasheet at MyBioSource, Recombinant Proteins. Application: SDS-PAGE
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1HVD: Structural and electrophysiological analysis of annexin V mutants. Mutagenesis of human annexin V, an in vitro voltage-gated calcium channel, provides information about the structural features of the ion pathway, the voltage sensor and the ion selectivity filter
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Complete information for ANXA5 gene (Protein Coding), Annexin A5, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Complete information for ANXA1 gene (Protein Coding), Annexin A1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Annexin A2 is a calcium regulated protein involved in membrane binding and actin polymerization. In this paper, Kevin Moreau and colleagues identified Annexin A2 as a…. ...
www.ncbi.nlm.nih.gov/pmc/articles/PMC3063939. Our results indicate that 4-1BB co-stimulation may significantly improve TIL survival during ... mAb from Bristol Myers Squibb (BMS) currently being tested in clinical trials,. ... BMS-663513 was at 14.9 mg/ml and was stored at 4°C. This Ab was .... The cells were then stained with anti-CD8-Pacific Blue, Annexin V-PE, and .... ...
E1 beta subunit of the complex forming an heterotetramer alpha2beta2 bound to two TPP (thiamine-pyrophosphate) one each bridging E1 alpha and E1 beta to form an active site catalyzing the irreversible decarboxylation of pyruvate to acetyl- ...
Annexin A1, also known as lipocortin I, is a protein that is encoded by the ANXA1 gene in humans. Annexin A1 belongs to the annexin family of Ca2+-dependent phospholipid-binding proteins that have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin A1 protein has an apparent relative molecular mass of 40 kDa with phospholipase A2 inhibitory activity. Glucocorticoids (such as budesonide, cortisol, and beclomethasone) are a class of endogenous or synthetic anti-inflammatory steroid hormones that bind to the glucocorticoid receptor (GR), which is present in almost every vertebrate animal cell. They are used in medicine to treat diseases caused by an overactive immune system, including allergies, asthma, autoimmune diseases, and sepsis. Because they suppress inflammatory pathways, long-term use of glucocorticoid drugs can lead to side-effects such as immunodeficiency and adrenal insufficiency. The main mechanism of ...
The surface of healthy cells is composed of lipids that are asymmetrically distributed on the inner and outer leaflet of the plasma membrane. One of these lipids, phosphatidylserine (PS), is normally restricted to the inner leaflet of the plasma membrane and is, therefore, only exposed to the cell cytoplasm. However, during apoptosis lipid asymmetry is lost and PS becomes exposed on the outer leaflet of the plasma membrane. Annexin V, a 36-kDa calcium-binding protein, binds to PS; therefore, fluorescently labeled Annexin V can be used to detect PS that is exposed on the outside of apoptotic cells. Annexin V can also stain necrotic cells because these cells have ruptured membranes that permit Annexin V to access the entire plasma membrane. However, apoptotic cells can be distinguished from necrotic cells by co-staining with propidium iodide (PI) because PI enters necrotic cells but is excluded from apoptotic cells. This protocol describes Annexin V binding and PI uptake followed by flow cytometry ...
The discovery of an essential role for annexin 11 in cell division is unexpected when considered in the broader context of annexin biology. Although annexins are now firmly implicated in various cellular activities, including endocytosis, calcium signaling, and apoptosis, no member of this protein family has been demonstrated to function in the cell cycle. The membrane binding and vesicle fusogenic properties that define annexins in biochemical terms could provide clues as to the possible role of annexin 11 in the last stage of cytokinesis. Before cells can be completely separated in a process known as abscission, the actomyosin constriction ring at the cleavage furrow has to be disassembled and the central spindle must separate. During this series of events, it is likely that the plasma membrane must be tightly attached to the midbody microtubules for abscission to be successfully completed. CHO1 has previously been suggested to be responsible for this connection (Kuriyama et al., 2002), but ...
Order monoclonal and polyclonal Annexin VIII antibodies for many applications. Selected quality suppliers for anti-Annexin VIII antibodies.
Helen Christians research interests are in the mechanisms of steroid hormone regulation of the pituitary gland, in particular the role of Annexin 1. The Annexins are a well-conserved super-family of structurally related Ca2+ - and phospholipids-binding proteins with wide-ranging functions in health and disease. Annexin 1 is a 37kD protein that is induced by glucocorticoids and mediates glucocorticoid action within the host defence and neuroendocrine systems. Glucocorticoids regulate the synthesis, phosphorylation and cellular disposition of annexin 1, and annexin 1 is implicated in the regulation by these important drugs of pituitary function, the control of cell growth and signal transduction. Most recently her group have characterized a novel secretory pathway of annexin 1, a protein which lacks a signal sequence, involving a member of the ATP binding cassette transporter family, ABCA1. This is the first time the secretion mechanism of an annexin family member has been determined and the ...
Modified annexin proteins, including a homodimer of human annexin V, are provided. Methods for their use, such as to prevent thrombosis without increasing hemorrhage, enhancing the survivability of platelets during storage or transfusion and to attenuate ischemia-reperfusion injury (IPI), are also provided. The modified annexins bind phosphatidylserine (PS) on cell surfaces, thereby preventing the assembly of the prothrombinase complex. The modified annexin decreases the binding of leukocytes and platelets during post-ischemic reperfusion, thereby restoring microvascular blood flow and decreasing organ damage. In addition, the modified annexin prevents lipid loss from platelets during storage.
Proteolytic cleavage of haemagglutinin (HA) is essential for the infectivity of influenza A viruses (IAVs). This is usually mediated by trypsin-like proteases present in the respiratory tract. However, the ability to use plasminogen (PLG) as an alternative protease may contribute to pathogenesis of IAV infections and virus replication outside the respiratory tract. It was demonstrated previously that neuraminidase (NA) of the IAV strain A/WSN/33 can sequester PLG, allowing this virus to replicate in a PLG-dependent fashion. However, PLG also promotes replication of other IAVs, although its mode of action is poorly understood. Here, using NA-deficient viruses, we demonstrate that NA is not required for the binding of PLG and subsequent cleavage of HA. However, we demonstrate that the cellular protein annexin 2 (A2) can bind PLG and contributes to PLG-dependent cleavage of HA and subsequent IAV replication. Collectively, these results indicate that PLG promotes IAV replication in an A2-dependent fashion
A idea behind this research is: if we know what parts of the bacteria stimulate healing, perhaps doctors can deliver that material, or something very close, to patients directly to treat intestinal diseases such as Crohns or ulcerative colitis.. This idea has advanced experimentally, as demonstrated by two papers from Jones and Neish’s frequent collaborator, Asma Nusrat, who recently moved from Emory to the University of Michigan. This team had shown that a protein produced by human intestinal cells called annexin A1 activates ROS, acting through the same N-formyl peptide receptors that bacteria do.. Nusrat told me Friday her team began investigating annexins a decade ago at Emory, and it was fortuitous that Neish was working on beneficial bacteria right down the hall, since it is now apparent that annexin A1 and the bacteria are activating the same molecular signals. (Did you know there is an entire conference devoted to annexins? I didnt until a few days ago.). In a ...
For those of you who were following the Caspase-3 thread and those of you who contacted me directly, heres the information regarding Caspase-3 and Annexin V that I received from Pharmingen. Any furthur questions, please contact Padma at Pharmingen. Padma was most helpful. Lora , -----Original Message----- , From: pkodukula at pharmingen.com [SMTP:pkodukula at pharmingen.com] , Sent: Monday, October 04, 1999 10:14 AM , To: Barsky, Lora , Subject: RE: caspase-3 , , , , Dear Lora, , , Thank you for your interest in PharMingens products. This is in reply to , your , question about the caspase 3 and the annexin V staining. We are releasing , a kit , for the BrDU and cytokine staining. But the annexin V is also known to , work , with the caspase 3. This is not something that we routinely quality , control in , our company but we have had input from customers who have used this , method. The , annexin V binding to phosphatidyl serine is dependent on the CA++ ion , concentration and so a Ca++ ...
Auranofin promotes mitochondrial apoptosis by inducing annexin A5 expression and translocation in human prostate cancer cells.: Auranofin is a lipophilic gold c
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1BCW: Mutational and crystallographic analyses of interfacial residues in annexin V suggest direct interactions with phospholipid membrane components.
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Tinción con anexina V y propidio yoduro (PI) ofrece a los investigadores una manera de identificar distintos tipos de muerte celular:...
Sigma-Aldrich offers abstracts and full-text articles by [Jakob Voelkl, Ioana Alesutan, Tatsiana Pakladok, Robert Viereck, Martina Feger, Sobuj Mia, Tanja Schönberger, Angelika A Noegel, Meinrad Gawaz, Florian Lang].
Mouse anti Human Annexin A3 antibody, clone 4F1 recognizes human annexin A3, also known as Annexin III, Inositol 1,2-cyclic phosphate 2-ph
TY - JOUR. T1 - Annexins family. T2 - Insights into their functions and potential role in pathogenesis of sarcoidosis. AU - Mirsaeidi, Mehdi. AU - Gidfar, Sanaz. AU - Vu, Ann. AU - Schraufnagel, Dean. PY - 2016. Y1 - 2016. N2 - Annexins are Ca2+-regulated phospholipid-binding proteins that play an important role in the cell life cycle, exocytosis, and apoptosis. Annexin A11 is one of the oldest vertebrate annexins that has a crucial role in sarcoidosis pathogenesis. The mechanism of effect in sarcoidosis granuloma cells may be due to alterations in apoptosis. Immune cells with a specific mutation at protein location 230 are resistant to apoptosis and consequently have continued effects on inflammation and progression of sarcoidosis. The mechanism of action of annexin A11 may be based upon alterations in delivering calcium to two different apoptosis pathways (caspase and P53).. AB - Annexins are Ca2+-regulated phospholipid-binding proteins that play an important role in the cell life cycle, ...
BACKGROUND Chronic hepatitis C virus (HCV) infection is characterized by a severe impairment of HCV-specific CD4+ T cell help that is driven by chronic antigen stimulation. We aimed to study the fate of HCV-specific CD4+ T cells after virus elimination.METHODS HCV-specific CD4+ T cell responses were longitudinally analyzed using MHC class II tetramer technology, multicolor flow cytometry, and RNA sequencing in a cohort of patients chronically infected with HCV undergoing therapy with direct-acting antivirals. In addition, HCV-specific neutralizing antibodies and CXCL13 levels were analyzed.RESULTS We observed that the frequency of HCV-specific CD4+ T cells increased within 2 weeks after initiating direct-acting antiviral therapy. Multicolor flow cytometry revealed a downregulation of exhaustion and activation markers and an upregulation of memory-associated markers. Although cells with a Th1 phenotype were the predominant subset at baseline, cells with phenotypic and transcriptional ...
Chronic hepatitis C virus (HCV) infection is characterized by persistent high-level viremia and defective cellular immunity, including a lack of functional HCV-specific CD4+ T cells. We previously described an exceptional period of viral control that occurs in some chronically infected women after childbirth. Here, we investigated whether reduced HCV replication after pregnancy is associated with recovery of CD4+ T cell immunity. Class II tetramer analysis revealed significantly greater frequencies of circulating HCV-specific CD4+ T cells at 3 months postpartum in women with concurrent declines in viremia compared with those with stable viremia. These HCV-specific CD4+ T cells had an effector-memory phenotype. Inhibitory coreceptor expression on these cells corresponded to the degree of viral control. Circulating CD4+ T cells produced IL-2 and IFN-γ after HCV antigen stimulation, demonstrating Th1 functionality. These data provide direct evidence that the profound loss of HCV-specific CD4+ T ...
Gliomas are highly invasive brain tumors with the occurrence of numerous microglia/macrophages (MG/MP) in and around the tumor. Annexin A2 is overexpressed in many cancers and correlates with increased plasmin activity on the tumor cell surface. Plasmin mediates degradation of extracellular matrix and angiogenesis to facilitate tumor growth. In this study, we used a mouse glioma cell line, GL261-EGFP, and stable clones transfected with an annexin A2 knockdown (annA2KD) construct, GL261-EGFP-annA2KD. We found that the annA2KD decreased glioma cell migration in vitro and decreased membrane-bound plasmin activity. In vivo we injected GL261-EGFP cells into the mouse brain and the glioma progression was followed. Knockdown of annexin A2 in glioma cells decreased tumor size and slowed down tumor progression, characterized by decreased invasion, angiogenesis and proliferation, as well as increased apoptosis in tumor tissue of the annA2KD group. We also investigated the interaction between glioma and ...
Annexin A1 / ANXA1, 0.1 ml. Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane.
Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. The alternative splicing event is tissue specific and the mRNA containing the cassette exon is prevalent in brain, heart and skeletal muscle. The transcripts also differ in their 3-non coding regions by the use of two alternative poly(A) signals. Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, ...
Annexin A6 (AnxA6) has been implicated in the regulation of endo-/exocytic pathways, cholesterol transport, and the formation of multifactorial signaling complexes in many different cell types. More recently, AnxA6 has also been linked to triglyceride storage in adipocytes. Here we investigated the potential role of AnxA6 in fatty acid (FA) induced lipid droplet (LD) formation in hepatocytes. AnxA6 was associated with LD from rat liver and HuH7 hepatocytes. In oleic acid (OA) -loaded HuH7 cells, substantial amounts of AnxA6 bound to LD in a Ca2+-independent manner. Remarkably, stable or transient AnxA6 overexpression in HuH7 cells led to elevated LD numbers/size and neutral lipid staining under control conditions as well as after OA loading compared to controls. In contrast, overexpression of AnxAl, AnxA2 and AnxA8 did not impact on OA-induced lipid accumulation. On the other hand, incubation of AnxA6-depleted HuH7 cells or primary hepatocytes from AnxA6 KO-mice with OA led to reduced FA ...
Annexin A2 (AnxA2) is a multi-functional and -compartmental protein whose subcellular localisation and functions are tightly regulated by its post-translational modifications. AnxA2 and its Tyr23-phosphorylated form (pTyr23AnxA2) are involved in malignant cell transformation, metastasis and angiogenesis. Here, we show that H2O2 exerts rapid, simultaneous and opposite effects on the Tyr23 phosphorylation status of AnxA2 in two distinct compartments of rat pheochromocytoma (PC12) cells. Reactive oxygen species induce dephosphorylation of pTyr23AnxA2 located in the PML bodies of the nucleus, whereas AnxA2 associated with F-actin at the cell cortex is Tyr23 phosphorylated. The H2O2-induced responses in both compartments are transient and the pTyr23AnxA2 accumulating at the cell cortex is subsequently incorporated into vesicles and then released to the extracellular space. Blocking nuclear export by leptomycin B does not affect the nuclear pool of pTyr23AnxA2, but increases the amount of total AnxA2 ...
Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 has 45 to 59% identity with other members of its family and shares a similar size and exon-intron organization. Isolated from human placenta, ANX4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells ...
ANXA2, a member of the annexin family, is a calcium-dependent phospholipid-binding protein that plays a role in the regulation of cellular growth and in signal transduction pathways. Its affinity for calcium is greatly enhanced by anionic phospholipids, and it binds two calcium ions with high affinity. ANXA2 may be involved in heat-stress response. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. It may also cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis.
Annexxin of 369 aas. Schistosomiasis, a major parasitic disease of humans, is second only to malaria in its global impact. The disease is caused by digenean trematodes that infest the vasculature of their human hosts. These flukes are limited externally by a body wall composed of a syncytial epithelium, the apical surface membrane, a parasitism-adapted dual membrane complex. Annexins are important for the stability of this apical membrane system. Leow et al. 2013 presented the first structural and immunobiochemical characterization of an annexin from Schistosoma mansoni. The crystal structures of annexin B22 (4MDV and 4MDU) in the apo and Ca2+ bound forms confirmed the presence of the previously predicted α-helical segment in the II/III linker and revealed a covalently linked head-to-head dimer. The dimeric arrangement revealed a non-canonical membrane binding site and a probable binding groove opposite the binding site. Annexin B22 expression correlated with life stages of the parasite that ...
Page contains details about annexin V conjugated to maleimide-functionalized Feraheme nanoparticles . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Annexin V-FITC Apoptosis assay results: Flow cytometry profiles represent Annexin-V-FITC staining in X-axis and PI in Y-axis: (a) control, i.e., neither PSi nor
Mouse Monoclonal Anti-Annexin A1 Antibody (2F1) [Alexa Fluor® 647]. Validated: WB, ELISA, IHC, IHC-P. Tested Reactivity: Human, Mouse, Rat, and more. 100% Guaranteed.
It has been reported that Annexin A2 (ANXA2) is up-regulated in hepatocellular carcinoma (HCC), but the roles of ANXA2 in the migration and invasion of HCC ...
ANXA1 - anti-ANXA1 (Annexin A1) mouse monoclonal antibody, clone OTI3A8 (formerly 3A8) available for purchase from OriGene - Your Gene Company.
Renshaw, Derek, Montero-Melendez, Trinidad, Dalli, Jesmond, Kamal, Ahmad, Brancaleone, Vincenzo, DAcquisto, Fulvio, Cirino, Giuseppe and Perretti, Mauro (2010) Downstream gene activation of the receptor ALX by the agonist Annexin A1. PLoS ONE, 5 (9). e12771. ISSN 1932-6203 ...
Annexin A1 (ANXA1) is a calcium- and phospholipid-binding protein and a known mediator of glucocorticoid-regulated inflammatory responses. Using a combined multiple high-throughput approach, we recently identified a reduced expression of ANXA1 in human breast cancer. The finding was confirmed at the gene level by quantitative reverse transcription-polymerase chain reaction and at the protein level by immunohistochemical staining of normal, benign, and malignant breast tissues. In this study, we constructed and used a high-density human breast cancer tissue microarray to characterize the expressional pattern of ANXA1 according to histopathologies. The tissue microarray contains 1,158 informative breast tissue cores of different histologies including normal tissues, hyperplasia, in situ and invasive tumors, and lymph node metastases. Our results showed that there was a significant decrease in glandular expression of ANXA1 in ductal carcinoma in situ and invasive ductal carcinoma compared with ...
Annexin A6 (AnxA6) is a Ca2+-dependent membrane-binding protein involved in vesicular traffic. The likely participation of AnxA6 in the response of lymphocytes to Ca2+ signals has not been investigated yet. The present study focuses on intracellular relocation of AnxA6 in human Jurkat T lymphoblasts upon stimulation followed by transient increase of intracellular [Ca2+] and exocytosis of interleukin-2 (IL-2). Stimulation of the cells under different experimental conditions (by lowering pH and/or by rising extracellular [Ca2+] in the presence of ionomycin) induced time-dependent transients of intracellular [Ca2+] and concomitant changes in AnxA6 intracellular localization and in IL-2 secretion, with only minor effects on cell viability and apoptosis. In resting conditions (in the presence of EGTA or with no ionophore) AnxA6 was localized uniformly in the cytosol, whereas it translocated to vesicular structures beneath the plasma membrane within 5 min following stimulation of Jurkat T cells and ...
The atherosclerotic process is considered to be driven by an imbalance between proand anti-inflammatory actions. Still, the inflammatory state in patients with coronary artery disease (CAD) remains to be clarified. Annexin A1 (AnxA1) is a glucocorticoidinduced protein which may have a key role in the anti-inflammatory response as a mediator of glucocorticoid effects.. The general aim of this thesis was to deepen the knowledge of pro- and antiinflammatory mechanisms in CAD via phenotypic assessments of immune cell subsets, in particular CD56+ T cells, and exploration of AnxA1. The long-term goal is to reveal basic mechanisms that will lead to the development of biomarkers, which may be used for individualized treatment and monitoring.. The AnxA1 protein was constitutively expressed in both neutrophils and peripheral blood mononuclear cells (PBMCs). However, it varied considerably across PBMC subsets, being most abundantly expressed in monocytes. The AnxA1 expression was also higher in CD56+ T ...
Antiphospholipid syndrome is associated with an increased risk of thrombosis and pregnancy loss. Annexin A5 (Anxa5) is a candidate autoantigen. It is not known, however, whether endogenous Anxa5 prevents foetal loss during normal pregnancy. We found
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AAD positive means late apoptosis? - posted in Flow Cytometry: We have a discussion in the lab about apoptosis detection by annexin V/AAD labeling. Many people say that Annexin V+/AAD+ cells must not be considered apoptotic but necrotic. I agree, provided that the apoptotic stimulus was rather short (for example 2 hours-staurosporine). However, when the stimulus lasts for 24 hours (serum starvation for instance) I think that cells which started the apoptotic process shortly after the start...
Phospholipid-binding protein EhC2A mediates calcium-dependent translocation of transcription factor URE3-BP to the plasma membrane of Entamoeba histolytica ...
Purified Recombinant Human ANXA2 293 Cell Lysate from Creative Biomart. Recombinant Human ANXA2 293 Cell Lysate can be used for research.
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Recent large 3 cohorts have shown that caffeinated beverage consumption was associated with lower risk of kidney stone disease. However, its protective mechanisms remained unknown and had not been previously investigated. We thus evaluated protective effects of caffeine (1 μM-10 mM) on calcium oxalate monohydrate (COM) kidney stone formation, using crystallization, crystal growth, cell-crystal adhesion, Western blotting, and immunofluorescence assays. The results showed that caffeine reduced crystal number but, on the other hand, increased crystal size, resulting in unchanged crystal mass, consistent with crystal growth that was not affected by caffeine. However, caffeine significantly decreased crystal-binding capacity of MDCK renal tubular cells in a dose-dependent manner. Western blotting and immunofluorescence study of COM crystal-binding proteins revealed significantly decreased level of annexin A1 on apical surface and its translocation into cytoplasm of the caffeine-treated cells, but no
Apoptosis, or programmed cell death, is a general mechanism for removal of unwanted cells from the immune system. It is characterized by chromatin condensation, a reduction in cell volume, and endonuclease cleavage of DNA into oligonucleosomal length fragments. Apoptosis is also accompanied by a loss of membrane phospholipid asymmetry, resulting in the exposure of phosphatidylserine at the surface of the cell. Expression of phosphatidylserine at the cell surface plays an important role in the recognition and removal of apoptotic cells by macrophages. Here we describe a new method for the detection of apoptotic cells by flow cytometry, using the binding of fluorescein isothiocyanate-labeled annexin V to phosphatidylserine. When Burkitt lymphoma cell lines and freshly isolated germinal center B cells are cultured under apoptosis inducing conditions, all cells showing chromatin condensation strongly stain with annexin V, whereas normal cells are annexin V negative. Moreover, DNA fragmentation is ...
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis. Alternatively spliced transcript variants have been described. [provided by RefSeq, Aug 2010 ...
Relative or absolute hypoxia activates signaling pathways that alter gene expression and stabilize the pulmonary microvasculature. Alveolar hypoxia occurs in disorders ranging from altitude sickness to airway obstruction, apnea, and atelectasis. Here, we report that the phospholipid-binding protein, annexin A2 (ANXA2) functions to maintain vascular integrity in the face of alveolar hypoxia. We demonstrate that microvascular endothelial cells (ECs) from Anxa2−/− mice display reduced barrier function and excessive Src-related tyrosine phosphorylation of the adherens junction protein vascular endothelial cadherin (VEC). Moreover, unlike Anxa2+/+ controls, Anxa2−/− mice develop pulmonary edema and neutrophil infiltration in the lung parenchyma in response to subacute alveolar hypoxia. Mice deficient in the ANXA2-binding partner, S100A10, failed to demonstrate hypoxia-induced pulmonary edema under the same conditions. Further analyses reveal that ANXA2 forms a complex with VEC and its ...
Annexin A1 (AnxA1), a phospholipid-binding protein and known regulator of glucocorticoid-induced inflammatory signaling, has been implicated for its role in human cancers, including head and neck cancer, colon, breast and prostate cancers. To determine the role of AnxA1 found to be secreted by the cancer-associated fibroblasts (CAF) of prostatic adenocarcinoma, we used primary cultures of CAFs, primary epithelial cultures and an epithelial cell line (cE1), all derived from the cPten-/-L mouse model of prostate adenocarcinoma in a series of experiments described here. The results of this dissertation point to a novel function of AnxA1 in prostate tumorigenesis and the establishment of a Cancer Cell-EMT-CSC lineage. It is projected that the presence of AnxA1 in the tumor microenvironment contributes to tumor stem cell activity via two separate but complementary pathways, first by induction of a de-differentiation process leading to generation of basal stem-like cells from a subpopulation of cancer ...
TY - JOUR. T1 - Assessment of secondary necrosis of Jurkat cells using a new microscopic system and double staining method with annexin V and propidium iodide.. AU - Honda, O.. AU - Kuroda, Masahiro. AU - Joja, I.. AU - Asaumi, Jun-Ichi. AU - Takeda, Yoshihiro. AU - Akaki, S.. AU - Togami, I.. AU - Kanazawa, Susumu. AU - Kawasaki, S.. AU - Hiraki, Y.. PY - 2000/2. Y1 - 2000/2. N2 - Using a new system developed by us for acquiring microscopic images automatically, we compared the morphological changes that apoptotic cells undergo with changes in the staining pattern of annexin V-enhanced green fluorescent protein (AV-EGFP) and propidium iodide (PI) in individual cells. Jurkat cells were treated with 5 mM CaCl2 alone, anti-Fas antibody and heating at 42 degrees C for 30 min or 46 degrees C for 60 min, and then were incubated in medium with 5 mM CaCl2. Time-lapse DNA fragmentation analysis and morphological observation revealed that the anti-Fas antibody and heating at 42 degrees C for 30 min ...
Cancer of the gingivo-buccal complex (GBC) is a major cancer in Indian men. This study reports the identification of tumor antigens, which elicit an antibody response in cancer of GBC using immunoproteomics. Proteins from KB cells separated by 2-D PAGE, were immunoblotted with IgG from sera of 28 cancer patients, 12 patients with leukoplakia, and 28 healthy individuals. Antigens detected by the IgGs from the patients sera were different among different individuals with presence of any single antigen ranging from 7 to 79%. Several of these antigens have been identified by MS and confirmed by immunostaining. They are three forms of α-enolase, peroxiredoxin-VI, annexin-II, HSP70, pyruvate kinase, α-tubulin, β-tubulin, ATP-synthase, phosphoglycerate mutase (PGM), aldose reductase, triosephosphate isomerase, and cyclophilin-A. Except, HSP70, these antigens are being reported in cancer of GBC for the first time. Pyruvate kinase and aldose reductase have not been reported to elicit autoantibody ...
AnxA1 is a molecule that belongs to a large and structurally heterogeneous family of proresolving molecules. Other family members include resolving lipid mediators, proresolving cytokines, and proresolving hormones. Although these molecules are structurally different they share functional similarities. They terminate inflammatory leukocyte accumulation by reducing leukocyte recruitment and enhancing egress; they stimulate clearance of aged and apoptotic cells and actively promote tissue repair.16 Here, we show that AnxA1 is an endogenous inhibitor of arterial leukocyte recruitment. Because this process is a mechanism essential to atherogenesis, it is not surprising that mice lacking AnxA1 or its receptor FPR2 have accelerated atherosclerosis. Mechanistically, we show that the AnxA1 fragment Ac2-26 counteracts chemokine-mediated integrin activation in neutrophils and monocytes and could hence be a potential tool for the treatment of atherosclerosis. Direct interference with integrins, cell ...
Alpha-giardins constitute the annexin proteome (group E annexins) in the intestinal protozoan parasite Giardia and, as such, represent the evolutionary oldest eukaryotic annexins. The dominance of alpha-giardins in the cytoskeleton of Giardiawith its greatly reduced actin content emphasises the importance of the alpha-giardins for the structural integrity of the parasite, which is particularly critical in the transformation stage between cyst and trophozoite. In this study, we report the crystal structures of the apo- and calcium-bound forms of a1-giardin, a protein localised to the plasma membrane of Giardia trophozoites that has recently been identified as a vaccine target. The calcium-bound crystal structure of a1-giardin revealed the presence of a type III site in the first repeat as known from other annexin structures, as well as a novel calcium binding site situated between repeats I and IV. By means of comparison, the crystal structures of three different alpha-giardins known to date ...
Multiple species of bacteria are able to sequester the host zymogen plasminogen to the cell surface. Once localised to the bacterial surface, plasminogen can act as a cofactor in adhesion, or, following activation to plasmin, provide a source of potent proteolytic activity. Numerous bacterial plasminogen receptors have been identified, and the mechanisms by which they interact with plasminogen are diverse. Here we provide an overview of bacterial plasminogen receptors and discuss the diverse role bacterial plasminogen acquisition plays in the relationship between bacteria and the host.
TY - JOUR. T1 - The membrane phospholipid binding protein annexin A2 promotes phagocytosis and nonlytic exocytosis of cryptococcus neoformans and impacts survival in fungal infection. AU - Stukes, Sabriya. AU - Coelho, Carolina. AU - Rivera, Johanna. AU - Jedlicka, Anne E.. AU - Hajjar, Katherine A.. AU - Casadevall, Arturo. PY - 2016/8/15. Y1 - 2016/8/15. N2 - Cryptococcus neoformans is a fungal pathogen with a unique intracellular pathogenic strategy that includes nonlytic exocytosis, a phenomenon whereby fungal cells are expunged from macrophages without lysing the host cell. The exact mechanism and specific proteins involved in this process have yet to be completely defined. Using murine macrophages deficient in the membrane phospholipid binding protein, annexin A2 (ANXA2), we observed a significant decrease in both phagocytosis of yeast cells and the frequency of nonlytic exocytosis. Cryptococcal cells isolated from Anxa2-deficient (Anxa2-/-) bone marrow-derived macrophages and lung ...
Lipid droplets are vital to hepatitis C virus (HCV) infection as the putative sites of virion assembly, but morphogenesis and egress of virions remain ill defined. We performed quantitative lipid droplet proteome analysis of HCV-infected cells to identify co-factors of that process. Our results demonstrate that HCV disconnects lipid droplets from their metabolic function. Annexin A3 (ANXA3), a protein enriched in lipid droplet fractions, strongly impacted HCV replication and was characterized further: ANXA3 is recruited to lipid-rich fractions in HCV-infected cells by the viral core and NS5A proteins. ANXA3 knockdown does not affect HCV RNA replication but severely impairs virion production with lower specific infectivity and higher density of secreted virions. ANXA3 is essential for the interaction of viral envelope E2 with apolipoprotein E (ApoE) and for trafficking, but not lipidation, of ApoE in HCV-infected cells. Thus, we identified ANXA3 as a regulator of HCV maturation and egress. ...

anti-Annexin II antibody  | GeneTexanti-Annexin II antibody | GeneTex

... annexin A2) for ICC/IF, IHC-P, IP, RipA, WB. Anti-Annexin II pAb (GTX32446) is tested in Human, Mouse, Rat samples. 100% Ab- ... annexin A2. Background. This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid- ... LIP2 Antibody , HEL-S-270 Antibody , LPC2 Antibody , CAL1H Antibody , annexin A2 Antibody , ANXA2 Antibody , ANX2 Antibody , ...
more infohttp://www.genetex.com/Annexin-II-antibody-GTX32446.html

ANXA2 - EDRN Public PortalANXA2 - EDRN Public Portal

... a member of the annexin family, is a calcium-dependent phospholipid-binding protein that plays a role in the regulation of ... ANXA2, a member of the annexin family, is a calcium-dependent phospholipid-binding protein that plays a role in the regulation ...
more infohttps://edrn.nci.nih.gov/biomarkers/ANXA2

Annexins sense changes in intracellular pH during hypoxia | Biochemical JournalAnnexins sense changes in intracellular pH during hypoxia | Biochemical Journal

The dimerization of annexin A2 within the annexin A2-S100A10 complex is essential for the pH-dependent membrane interaction at ... Our results show that annexin A6 and the heterotetramer A2-S100A10 (but not annexins A1, A4 and A5) interact independently of ... We show that hypoxia promotes the interaction of the recombinant annexin A2-S100A10 (p11) and annexin A6 with the plasma ... We have investigated in vivo the influence of the pHi on the membrane association of human annexins A1, A2, A4, A5 and A6 ...
more infohttp://www.biochemj.org/content/409/1/65

Calcium- and proton-dependent relocation of annexin A6 in Jurkat T cells stimulated for interleukin-2 secretion - Acta...Calcium- and proton-dependent relocation of annexin A6 in Jurkat T cells stimulated for interleukin-2 secretion - Acta...

Casas J, Gijon MA, Vigo AG, Crespo MS, Balsinde J, Balboa MA (2006) Overexpression of cytosolic group IVA phospholipase A2 ... GTP-induced membrane binding and ion channel activity of annexin VI: is annexin VI a GTP biosensor? Biophys J 82: 2737-2745. ... Annexin A6 (AnxA6) is a Ca2+-dependent membrane-binding protein involved in vesicular traffic. The likely participation of ... Golczak M, Kicinska A, Bandorowicz-Pikula J, Buchet R, Szewczyk A, Pikula S (2001a) Acidic pH-induced folding of annexin VI is ...
more infohttp://psjd.icm.edu.pl/psjd/element/bwmeta1.element.bwnjournal-article-abpv54p261kz

Anti-Annexin A2 antibody (ab2242) | AbcamAnti-Annexin A2 antibody (ab2242) | Abcam

Goat polyclonal Annexin A2 antibody validated for WB and tested in Human. Referenced in 2 publications and 1 independent review ... Anti-Annexin A2 antibody (ab2242) at 0.5 µg/ml + A431 lysate in RIPA buffer at 35 µg. Developed using the ECL technique.. ... Detects a band of approximately 35 kDa (predicted molecular weight: 38 kDa).Can be blocked with Human Annexin A2 peptide ( ... A pair of annexin repeats may form one binding site for calcium and phospholipid. ...
more infohttp://www.abcam.com/annexin-a2-antibody-ab2242.html

ANXA2P3 annexin A2 pseudogene 3 [Homo sapiens (human)] - Gene - NCBIANXA2P3 annexin A2 pseudogene 3 [Homo sapiens (human)] - Gene - NCBI

ANXA2P3 annexin A2 pseudogene 3 [Homo sapiens] ANXA2P3 annexin A2 pseudogene 3 [Homo sapiens]. Gene ID:305 ... Role of the long non‑coding RNA‑Annexin A2 pseudogene 3/Annexin A2 signaling pathway in biliary atresia‑associated hepatic ... Title: Role of the long non‑coding RNA‑Annexin A2 pseudogene 3/Annexin A2 signaling pathway in biliary atresia‑associated ... annexin A2 pseudogene 3provided by HGNC. Primary source. HGNC:HGNC:540 Gene type. pseudo. RefSeq status. PROVISIONAL. Organism ...
more infohttps://www.ncbi.nlm.nih.gov/gene/305

Anti-Annexin A2 antibody [M298] (ab86709) | AbcamAnti-Annexin A2 antibody [M298] (ab86709) | Abcam

Mouse monoclonal Annexin A2 antibody [M298] validated for WB, IP, ELISA and tested in Rat. Immunogen corresponding to ... Lane 1 : Anti-Annexin A2 antibody [M298] (ab86709) at 1/250 dilution. Lane 2 : Anti-Annexin A2 antibody [M298] (ab86709) at 1/ ... A pair of annexin repeats may form one binding site for calcium and phospholipid. ... Recombinant fragment, corresponding to the C-terminal region of Rat Annexin II ...
more infohttp://www.abcam.com/annexin-a2-antibody-m298-ab86709.html

Annexin A2 Antibody (NBP1-31310): Novus BiologicalsAnnexin A2 Antibody (NBP1-31310): Novus Biologicals

Rabbit Polyclonal Anti-Annexin A2 Antibody. Validated: WB, ICC/IF, IHC, IHC-P, IP. Tested Reactivity: Human, Mouse, Rat, and ... Reviews for Annexin A2 Antibody (NBP1-31310) (0) There are no reviews for Annexin A2 Antibody (NBP1-31310). By submitting a ... Diseases for Annexin A2 Antibody (NBP1-31310). Discover more about diseases related to Annexin A2 Antibody (NBP1-31310). ... PTMs for Annexin A2 Antibody (NBP1-31310). Learn more about PTMs related to Annexin A2 Antibody (NBP1-31310). ...
more infohttps://www.novusbio.com/products/annexin-a2-antibody_nbp1-31310

Annexin A2 - WikipediaAnnexin A2 - Wikipedia

Annexin A2 also known as annexin II is a protein that in humans is encoded by the ANXA2 gene. Annexin 2 is involved in diverse ... Kwon M, MacLeod TJ, Zhang Y, Waisman DM (Jan 2005). "S100A10, annexin A2, and annexin a2 heterotetramer as candidate ... "Entrez Gene: ANXA2 annexin A2". Kling T, Ferrarese R, Ó hAilín D, Johansson P, Heiland DH, Dai F, Vasilikos I, Weyerbrock A, ... Annexin A2 has been proposed to function inside the cell in sorting of endosomes and outside the cell in anticoagulant ...
more infohttps://en.wikipedia.org/wiki/Annexin_A2

anxa2 Protein, annexin A2 - Creative BioMartanxa2 Protein, annexin A2 - Creative BioMart

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a ... Recombinant Human Annexin A2, His-tagged. E. coli. Human. His. +Inquiry. ANXA2-1845H. Recombinant Human Annexin A2, His-tagged ... ANXA2; annexin A2; ANX2ANX2L4; CAL1H; LPC2D; LIP2; Annexin II; Annexin-2; Calpactin I heavy chain; Calpactin-1 heavy chain; ... Canas, F; Simonin, L; et al. Annexin A2 autoantibodies in thrombosis and autoimmune diseases. THROMBOSIS RESEARCH 135:226-230( ...
more infohttps://www.creativebiomart.net/symbolsearch_anxa2.htm

Annexin A2 expression during cellular differentiation in myeloid cell lines | Biochemical Society TransactionsAnnexin A2 expression during cellular differentiation in myeloid cell lines | Biochemical Society Transactions

Annexin A2 expression during cellular differentiation in myeloid cell lines. W.S. Gilmore, S. Olwill, H. McGlynn, H.D. ... Annexin A2 expression during cellular differentiation in myeloid cell lines. W.S. Gilmore, S. Olwill, H. McGlynn, H.D. ... Annexin A2 expression during cellular differentiation in myeloid cell lines Message Subject (Your Name) has forwarded a page to ... Annexin A2 is a calcium-dependent, phospholipid-binding protein found on many cell types. It consists of a short hydrophobic ...
more infohttp://www.biochemsoctrans.org/content/32/6/1122

IJMS | Free Full-Text | Regulation of von-Willebrand Factor Secretion from Endothelial Cells by the Annexin A2-S100A10 ComplexIJMS | Free Full-Text | Regulation of von-Willebrand Factor Secretion from Endothelial Cells by the Annexin A2-S100A10 Complex

Among them, the annexin A2 (AnxA2)-S100A10 complex has been shown to participate in the tethering/docking of secretion- ... Among them, the annexin A2 (AnxA2)-S100A10 complex has been shown to participate in the tethering/docking of secretion- ... "Regulation of von-Willebrand Factor Secretion from Endothelial Cells by the Annexin A2-S100A10 Complex." Int. J. Mol. Sci. 19, ... Regulation of von-Willebrand Factor Secretion from Endothelial Cells by the Annexin A2-S100A10 Complex. Anna Holthenrich. ...
more infohttps://www.mdpi.com/1422-0067/19/6/1752

Annexin A2 at the Interface of Actin and Membrane Dynamics: A Focus on Its Roles in Endocytosis and Cell PolarizationAnnexin A2 at the Interface of Actin and Membrane Dynamics: A Focus on Its Roles in Endocytosis and Cell Polarization

... Adam G. ... M. J. Hayes, D.-M. Shao, A. Grieve, T. Levine, M. Bailly, and S. E. Moss, "Annexin A2 at the interface between F-actin and ... D. B. N. Lee, N. Jamgotchian, S. G. Allen, F. W. K. Kan, and I. L. Hale, "Annexin A2 heterotetramer: role in tight junction ... E. M. Danielsen, B. van Deurs, and G. H. Hansen, "Nonclassical secretion of annexin A2 to the lumenal side of the enterocyte ...
more infohttps://www.hindawi.com/journals/ijcb/2012/852430/ref/

ANXA2 recombinant protein | Annexin A2 Recombinant Protein-NP 001002857ANXA2 recombinant protein | Annexin A2 Recombinant Protein-NP 001002857

Annexin A2 Recombinant Protein-NP_001002857 (MBS203687) product datasheet at MyBioSource, Recombinant Proteins. Application: ... Annexin A2 UniProt Synonym Protein Names Annexin II; Annexin-2; Calpactin I heavy chain; Calpactin-1 heavy chain; Chromobindin- ... annexin A2; annexin II; annexin-2; calpactin I heavy chain; calpactin I heavy polypeptide; calpactin-1 heavy chain; ... Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated ...
more infohttps://www.mybiosource.com/prods/Recombinant-Protein/Annexin-A2/ANXA2/datasheet.php?products_id=203687

Effects of Tissue Plasminogen Activator and Annexin A2 Combination Therapy on Long-Term Neurological Outcomes of Rat Focal...Effects of Tissue Plasminogen Activator and Annexin A2 Combination Therapy on Long-Term Neurological Outcomes of Rat Focal...

Background and Purpose-Tissue-type plasminogen activator (tPA) in combination with recombinant annexin A2 (rA2) is known to ... Effects of Tissue Plasminogen Activator and Annexin A2 Combination Therapy on Long-Term Neurological Outcomes of Rat Focal ... Effects of Tissue Plasminogen Activator and Annexin A2 Combination Therapy on Long-Term Neurological Outcomes of Rat Focal ... Effects of Tissue Plasminogen Activator and Annexin A2 Combination Therapy on Long-Term Neurological Outcomes of Rat Focal ...
more infohttp://stroke.ahajournals.org/content/early/2013/12/23/STROKEAHA.113.003823.short?rss=1

Reactive oxygen species exert opposite effects on Tyr23 phosphorylation of the nuclear and cortical pools of Annexin A2Reactive oxygen species exert opposite effects on Tyr23 phosphorylation of the nuclear and cortical pools of Annexin A2

... Type. ... Annexin A2 (AnxA2) is a multi-functional and -compartmental protein whose subcellular localisation and functions are tightly ...
more infohttp://bora.uib.no/handle/1956/11810

Characterization of interactions between hepatitis C virus NS5B polymerase, annexin A2 and RNA - effects on NS5B catalysis and...Characterization of interactions between hepatitis C virus NS5B polymerase, annexin A2 and RNA - effects on NS5B catalysis and...

Annexin A2 (AnxA2) has been reported to co-precipitate with the non-structural (NS) HCV proteins NS5B and NS3/NS4A, indicating ... Annexin A2, Hepatitis C virus, Hepatocellular carcinoma, NS5B, RNA, allosteric inhibition, filibuvir, surface plasmon resonance ... Characterization of interactions between hepatitis C virus NS5B polymerase, annexin A2 and RNA - effects on NS5B catalysis and ...
more infohttp://uu.diva-portal.org/smash/record.jsf?pid=diva2:1170189

anti-Annexin A2 antibody | biobool.comanti-Annexin A2 antibody | biobool.com

anti-Annexin A2 antibody Contact Us * Alternative name. Annexin 2, annexin A2, Annexin II, ANX2, ANX2L4, ANXA2, CAL1H, ... Product Description specificalAnnexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up- ... Inquiry About anti-Annexin A2 antibody If you hope to order it or contact us directly, please contact us via [email protected] ... Immunogenannexin A2 * IHCImmunohistochemistry of paraffin-embedded human breast cancer slide using A000431( ANXA2 Antibody) at ...
more infohttps://www.biobool.com/monoclonal_antibody/431.html

Annexin A2 (C-terminal region) - ECM BiosciencesAnnexin A2 (C-terminal region) - ECM Biosciences

Annexin A1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent ... The Annexin family of calcium-binding proteins is composed of al least thirteen mammalian genes ( ... Clone (M298) was generated from a recombinant sequence that included amino acids in the C-terminal region of rat Annexin A2. * ... Annexin A2 (calpactin I, chromobindin 8, p36, Lipocortin II, PAP-IV, or Protein I) is a cytoskeletal calcium-dependent ...
more infohttps://ecmbio.com/products/am2981

Phosphatidylserine Membrane Domain Clustering Induced by Annexin A2/S100A10 Heterotetramer
    †Phosphatidylserine Membrane Domain Clustering Induced by Annexin A2/S100A10 Heterotetramer †

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more infohttps://www.infona.pl/resource/bwmeta1.element.acs-doi-10_1021_bi051585i

Annexin A2 mediates apical trafficking of renal Na(+)-K(+)-2Cl(-)-cotransporter  - MDC RepositoryAnnexin A2 mediates apical trafficking of renal Na(+)-K(+)-2Cl(-)-cotransporter - MDC Repository

Yeast two-hybrid screening identified an interaction between NKCC2 and the cytosolic protein, annexin A2 (AnxA2). Annexins ... Annexin A2 mediates apical trafficking of renal Na(+)-K(+)-2Cl(-)-cotransporter. Creators Name:. Dathe, C. and Daigeler, A.L. ...
more infohttp://edoc.mdc-berlin.de/13871/

Abstract | Expression of circulating annexin A2 in hepatic diseases and hepatocellular carcinoma | Journal of Cancer...Abstract | Expression of circulating annexin A2 in hepatic diseases and hepatocellular carcinoma | Journal of Cancer...

Expression of circulating annexin A2 in hepatic diseases and hepatocellular carcinoma. Ebtesam El-Gezawy1, Laila Abdelbaki2, ... El-Gezawy E, Abdelbaki L, Deen M.Z, Eldeek S and Abdelgawad M.I. Expression of circulating annexin A2 in hepatic diseases and ... El-Gezawy E, Abdelbaki L, Deen M.Z, Eldeek S and Abdelgawad M.I. Expression of circulating annexin A2 in hepatic diseases and ... Annexin A2 (ANXA2) plays an important role in the pathogenesis of multiple malignancies particularly HCCs and its expression ...
more infohttp://www.hoajonline.com/jctr/abstract/2049-7962/6/2

Inside-out: Unpredicted Annexin A2 localisation on the surface of extracellular vesicles - MattersInside-out: Unpredicted Annexin A2 localisation on the surface of extracellular vesicles - Matters

Annexin A2 is a cytosolic protein abundant in EVs. We show for the first time that Annexin A2 is expressed not only in the ... This raises fundamental questions regarding Annexin A2 transport to the outer leaflet of the EV membrane as it lacks a signal ... Annexin A2 is a cytosolic protein abundant in EVs. We show for the first time that Annexin A2 is expressed not only in the ... Annexin A2 is a cytosolic protein abundant in EVs. We show for the first time that Annexin A2 is expressed not only in the ...
more infohttps://sciencematters.io/articles/201602000015

The membrane phospholipid binding protein annexin A2 promotes phagocytosis and nonlytic exocytosis of cryptococcus neoformans...The membrane phospholipid binding protein annexin A2 promotes phagocytosis and nonlytic exocytosis of cryptococcus neoformans...

Stukes S, Coelho C, Rivera J, Jedlicka AE, Hajjar KA, Casadevall A. The membrane phospholipid binding protein annexin A2 ... Using murine macrophages deficient in the membrane phospholipid binding protein, annexin A2 (ANXA2), we observed a significant ... Using murine macrophages deficient in the membrane phospholipid binding protein, annexin A2 (ANXA2), we observed a significant ... Using murine macrophages deficient in the membrane phospholipid binding protein, annexin A2 (ANXA2), we observed a significant ...
more infohttps://einstein.pure.elsevier.com/en/publications/the-membrane-phospholipid-binding-protein-annexin-a2-promotes-pha
  • Immunohistochemistry-Paraffin: Annexin A2 Antibody [NBP1-- Analysis of paraffin-embedded Human pancreas tissue, using ANXA2 antibody at 1:100 dilution. (novusbio.com)
  • Annexin A2 also known as annexin II is a protein that in humans is encoded by the ANXA2 gene. (wikipedia.org)
  • Among them, the annexin A2 (AnxA2)-S100A10 complex has been shown to participate in the tethering/docking of secretion-competent WPB at the plasma membrane, and interference with AnxA2/S100A10 expression or complex formation significantly reduces acute WPB exocytosis and VWF release. (mdpi.com)
  • Annexin A2 (AnxA2) is a multi-functional and -compartmental protein whose subcellular localisation and functions are tightly regulated by its post-translational modifications. (uib.no)
  • Annexin A2 (AnxA2) has been reported to co-precipitate with the non-structural (NS) HCV proteins NS5B and NS3/NS4A, indicating a role in HCC tumorigenesis and effect on DAA therapy. (diva-portal.org)
  • Product Description specifical Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. (biobool.com)
  • Yeast two-hybrid screening identified an interaction between NKCC2 and the cytosolic protein, annexin A2 (AnxA2). (mdc-berlin.de)
  • Annexin A2 (ANXA2) plays an important role in the pathogenesis of multiple malignancies particularly HCCs and its expression strongly also affects the outcomes of HCC patients. (hoajonline.com)
  • Using murine macrophages deficient in the membrane phospholipid binding protein, annexin A2 (ANXA2), we observed a significant decrease in both phagocytosis of yeast cells and the frequency of nonlytic exocytosis. (elsevier.com)
  • Annexin A2 (ANXA2) is a pro-metastasis protein, previously identified as a relevant PDAC antigen that is expressed by a GM-CSF-secreting allogenic whole pancreatic tumor cell vaccine (GVAX) to induce an anti-ANXA2 antibody response in patients with PDAC. (biomedcentral.com)
  • Ferret Annexin A2/ANXA2 Gene ORF cDNA clone expression plasmid, C-GFPSpark tag (FG60086-ACG) - available for purchase from ABclonal - allows for easy subcloning of the ORF (pCMV3-C-GFPSpark Vector) to other plasmid vectors. (abclonal.com)
  • We aimed to determine whether annexin A2 (ANXA2), a Ca2 +-dependent and phospholipid-binding protein, participates in cell junction dynamics. (popcouncil.org)
  • It has been reported that Annexin A2 (ANXA2) is up-regulated in hepatocellular carcinoma (HCC), but the roles of ANXA2 in the migration and invasion of HCC cells have not been determined. (paperity.org)
  • Annexin A2 (ANXA2), also called Annexin II, is one of the best characterized of the Annexins. (paperity.org)
  • Annexin A2 (Anxa2) is a calcium-dependent phospholipid-binding protein found on various cell types that plays multiple roles in regulating cellular functions. (elsevier.com)
  • El-Gezawy E, Abdelbaki L, Deen M.Z, Eldeek S and Abdelgawad M.I. Expression of circulating annexin A2 in hepatic diseases and hepatocellular carcinoma . (hoajonline.com)
  • Analysis of CD44-containing lipid rafts: recruitment of annexin II and stabilization by the actin cytoskeleton," The Journal of Cell Biology , vol. 146, no. 4, pp. 843-854, 1999. (hindawi.com)
  • Annexin A2 has been implicated in a number of biochemical processes, including cell proliferation, foetal immune tolerance, ion-channel activation, cell-cell interactions and the bridging of membranes. (biochemsoctrans.org)
  • The name annexin is derived from the Greek annex, which means bring/hold together, and was chosen to describe the principal property of all, or at least nearly all, annexins the binding to and possibly holding together of certain biological structures, in particular membranes . (paperity.org)
  • T. Harder, R. Kellner, R. G. Parton, and J. Gruenberg, "Specific release of membrane-bound annexin II and cortical cytoskeletal elements by sequestration of membrane cholesterol," Molecular Biology of the Cell , vol. 8, no. 3, pp. 533-545, 1997. (hindawi.com)
  • There are at least ten different annexins in mammalian species. (mybiosource.com)
  • 2017. "Annexin A2 is critical for blood-testis barrier integrity and spermatid disengagement in the mammalian testis," Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 1864(3): 527-545. (popcouncil.org)
  • Detects a band of approximately 35 kDa (predicted molecular weight: 38 kDa).Can be blocked with Human Annexin A2 peptide (ab22880) . (abcam.com)
  • Role of the long non‑coding RNA‑Annexin A2 pseudogene 3/Annexin A2 signaling pathway in biliary atresia‑associated hepatic injury. (nih.gov)
  • This study aimed to investigate the clinical utility of hepatic and circulating Annexin A2 expression levels as a novel diagnostic marker of HCC and to correlate its level with alpha fetoprotein (AFP), the current marker ofHCC. (hoajonline.com)
  • Western blotting analysis and immunohistochemistry of tumor tissues and normal tissues from patients with UTUC were carried out to further verify five possible UTUC biomarkers, including zinc-alpha-2-glycoprotein, calreticulin, annexin A2, annexin A3 and haptoglobin. (beds.ac.uk)
  • Heat-stress response may also involve Annexin-2. (biobool.com)
  • Annexin A6 reverses transformation of A431 cells after overexpression, and this effect may involve annexin A6 targeting of p120 RasGAP to the plasma membrane to inactivate Ras. (ecmbio.com)
  • The results demonstrated that annexin A2 expression was affected when the cells were induced to differentiate by stimulation with all- trans -retinoic acid. (biochemsoctrans.org)
  • Epigenetic regulation of Annexin A2 has been identified as a key determinant of mesenchymal transformation in brain tumors. (wikipedia.org)
  • Characterization of interactions between hepatitis C virus NS5B polymerase, annexin A2 and RNA - effects on NS5B catalysis and allosteric inhibition. (diva-portal.org)
  • CEACAM1 has been shown to interact with PTPN11 and Annexin A2. (wikipedia.org)
  • Left: Diagram of the experimental concept, where trypsin cleaves surface bound Annexin A2. (sciencematters.io)
  • Immunocytochemical labeling of Annexin A2 in NGF-differentiated rat PC12 cells. (ecmbio.com)
  • The cells were fixed in paraformaldehyde and permeabilized using NP-40, then labeled with mouse monoclonal Annexin A2 (C-terminal region). (ecmbio.com)
  • Surface-bound Annexin A2 is digested by trypsin and cleavage products detected. (sciencematters.io)