A member of the annexin family that is a substrate for a tyrosine kinase, ONCOGENE PROTEIN PP60(V-SRC). Annexin A2 occurs as a 36-KDa monomer and in a 90-KDa complex containing two subunits of annexin A2 and two subunits of S100 FAMILY PROTEIN P11. The monomeric form of annexin A2 was formerly referred to as calpactin I heavy chain.
Protein of the annexin family exhibiting lipid interaction and steroid-inducibility.
A protein of the annexin family isolated from human PLACENTA and other tissues. It inhibits cytosolic PHOSPHOLIPASE A2, and displays anticoagulant activity.
Protein of the annexin family with a probable role in exocytotic and endocytotic membrane events.
Protein of the annexin family originally isolated from the electric organ of the electric ray Torpedo marmorata. It has been found in a wide range of mammalian tissue where it is localized to the apical membrane of polarized EPITHELIAL CELLS.
An annexin family member that plays a role in MEMBRANE FUSION and signaling via VOLTAGE-DEPENDENT CALCIUM CHANNELS.
A protein of the annexin family that catalyzes the conversion of 1-D-inositol 1,2-cyclic phosphate and water to 1-D-myo-inositol 1-phosphate.
Family of calcium- and phospholipid-binding proteins which are structurally related and exhibit immunological cross-reactivity. Each member contains four homologous 70-kDa repeats. The annexins are differentially distributed in vertebrate tissues (and lower eukaryotes) and appear to be involved in MEMBRANE FUSION and SIGNAL TRANSDUCTION.
A family of highly acidic calcium-binding proteins found in large concentration in the brain and believed to be glial in origin. They are also found in other organs in the body. They have in common the EF-hand motif (EF HAND MOTIFS) found on a number of calcium binding proteins. The name of this family derives from the property of being soluble in a 100% saturated ammonium sulfate solution.
Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a serine moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and serine and 2 moles of fatty acids.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Quaternary ammonium analog of ethidium; an intercalating dye with a specific affinity to certain forms of DNA and, used as diiodide, to separate them in density gradients; also forms fluorescent complexes with cholinesterase which it inhibits.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.
Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
A cell line derived from cultured tumor cells.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Extracellular vesicles generated by the shedding of CELL MEMBRANE blebs.
A family of G-protein-coupled receptors that was originally identified by its ability to bind N-formyl peptides such as N-FORMYLMETHIONINE LEUCYL-PHENYLALANINE. Since N-formyl peptides are found in MITOCHONDRIA and BACTERIA, this class of receptors is believed to play a role in mediating cellular responses to cellular damage and bacterial invasion. However, non-formylated peptide ligands have also been found for this receptor class.
Proteins prepared by recombinant DNA technology.
A short pro-domain caspase that plays an effector role in APOPTOSIS. It is activated by INITIATOR CASPASES such as CASPASE 9. Isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Organic compounds that contain technetium as an integral part of the molecule. These compounds are often used as radionuclide imaging agents.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Established cell cultures that have the potential to propagate indefinitely.
A family of intracellular CYSTEINE ENDOPEPTIDASES that play a role in regulating INFLAMMATION and APOPTOSIS. They specifically cleave peptides at a CYSTEINE amino acid that follows an ASPARTIC ACID residue. Caspases are activated by proteolytic cleavage of a precursor form to yield large and small subunits that form the enzyme. Since the cleavage site within precursors matches the specificity of caspases, sequential activation of precursors by activated caspases can occur.
Fluorescent probe capable of being conjugated to tissue and proteins. It is used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.
An in situ method for detecting areas of DNA which are nicked during APOPTOSIS. Terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 prime OH ends of either single- or double-stranded DNA. The terminal deoxynucleotidyl transferase nick end labeling, or TUNEL, assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA FRAGMENTATION.
A product of the lysis of plasminogen (profibrinolysin) by PLASMINOGEN activators. It is composed of two polypeptide chains, light (B) and heavy (A), with a molecular weight of 75,000. It is the major proteolytic enzyme involved in blood clot retraction or the lysis of fibrin and quickly inactivated by antiplasmins.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Molecules or ions formed by the incomplete one-electron reduction of oxygen. These reactive oxygen intermediates include SINGLET OXYGEN; SUPEROXIDES; PEROXIDES; HYDROXYL RADICAL; and HYPOCHLOROUS ACID. They contribute to the microbicidal activity of PHAGOCYTES, regulation of signal transduction and gene expression, and the oxidative damage to NUCLEIC ACIDS; PROTEINS; and LIPIDS.
A partitioning within cells due to the selectively permeable membranes which enclose each of the separate parts, e.g., mitochondria, lysosomes, etc.
Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.
A usually benign, well-encapsulated, lobular, vascular tumor of chromaffin tissue of the ADRENAL MEDULLA or sympathetic paraganglia. The cardinal symptom, reflecting the increased secretion of EPINEPHRINE and NOREPINEPHRINE, is HYPERTENSION, which may be persistent or intermittent. During severe attacks, there may be HEADACHE; SWEATING, palpitation, apprehension, TREMOR; PALLOR or FLUSHING of the face, NAUSEA and VOMITING, pain in the CHEST and ABDOMEN, and paresthesias of the extremities. The incidence of malignancy is as low as 5% but the pathologic distinction between benign and malignant pheochromocytomas is not clear. (Dorland, 27th ed; DeVita Jr et al., Cancer: Principles & Practice of Oncology, 3d ed, p1298)
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
A biosensing technique in which biomolecules capable of binding to specific analytes or ligands are first immobilized on one side of a metallic film. Light is then focused on the opposite side of the film to excite the surface plasmons, that is, the oscillations of free electrons propagating along the film's surface. The refractive index of light reflecting off this surface is measured. When the immobilized biomolecules are bound by their ligands, an alteration in surface plasmons on the opposite side of the film is created which is directly proportional to the change in bound, or adsorbed, mass. Binding is measured by changes in the refractive index. The technique is used to study biomolecular interactions, such as antigen-antibody binding.
Any of a variety of procedures which use biomolecular probes to measure the presence or concentration of biological molecules, biological structures, microorganisms, etc., by translating a biochemical interaction at the probe surface into a quantifiable physical signal.
Proteins encoded by a VIRAL GENOME that are produced in the organisms they infect, but not packaged into the VIRUS PARTICLES. Some of these proteins may play roles within the infected cell during VIRUS REPLICATION or act in regulation of virus replication or VIRUS ASSEMBLY.
A genus of FLAVIVIRIDAE causing parenterally-transmitted HEPATITIS C which is associated with transfusions and drug abuse. Hepatitis C virus is the type species.
INFLAMMATION of the LIVER in humans caused by HEPATITIS C VIRUS, a single-stranded RNA virus. Its incubation period is 30-90 days. Hepatitis C is transmitted primarily by contaminated blood parenterally, and is often associated with transfusion and intravenous drug abuse. However, in a significant number of cases, the source of hepatitis C infection is unknown.
A low affinity interleukin-5 receptor subunit that combines with the CYTOKINE RECEPTOR COMMON BETA SUBUNIT to form a high affinity receptor for INTERLEUKIN-5. Several isoforms of the interleukin-5 receptor alpha subunit exist due to multiple ALTERNATIVE SPLICING.
Agents used in the prophylaxis or therapy of VIRUS DISEASES. Some of the ways they may act include preventing viral replication by inhibiting viral DNA polymerase; binding to specific cell-surface receptors and inhibiting viral penetration or uncoating; inhibiting viral protein synthesis; or blocking late stages of virus assembly.

The Npc1 mutation causes an altered expression of caveolin-1, annexin II and protein kinases and phosphorylation of caveolin-1 and annexin II in murine livers. (1/431)

We have previously demonstrated (1) an increased expression of caveolin-1 in murine heterozygous and homozygous Niemann-Pick type C (NPC) livers, and (2) an increased concentration of unesterified cholesterol in a detergent insoluble caveolae-enriched fraction from homozygous livers. To define further the relationship between caveolin-1 function and the cholesterol trafficking defect in NPC, we examined the expression and distribution of additional caveolar and signal transduction proteins. The expression of annexin II was significantly increased in homozygous liver homogenates and the Triton X-100 insoluble floating fraction (TIFF). Phosphoamino acid analysis of caveolin-1 and annexin II from the homozygous TIFF demonstrated an increase in serine and tyrosine phosphorylation, respectively. To determine the basis for increased phosphorylation of these proteins, the expression and distribution of several protein kinases was examined. The expression of PKCalpha, PKCzeta and pp60-src (protein kinases) were significantly increased in both heterozygous and homozygous liver homogenates, while PKCdelta was increased only in homozygous livers. Of the protein kinases analyzed, only CK IIalpha was significantly enriched in the heterozygous TIFF. Finally, the concentration of diacylglycerol in the homozygous TIFF was significantly increased and this elevation may modulate PKC distribution and function. These results provide additional evidence for involvement of a caveolin-1 containing cellular fraction in the pathophysiology of NPC and also suggest that the Npc1 gene product may directly or indirectly, regulate the expression and distribution of signaling molecules.  (+info)

Tissue plasminogen activator and its receptor in the human amnion, chorion, and decidua at preterm and term. (2/431)

The plasminogen activator system consists of two proteins: tissue plasminogen activator (tPA) and urokinase plasminogen activator (uPA), which act upon their specific receptors to generate plasmin from plasminogen located on the cell surface. Plasmin then acts directly and indirectly to degrade the components of the extracellular matrix (ECM). This process is likely to be important in the normal turnover of the ECM of fetal membranes and in its premature weakening in preterm premature rupture of the fetal membranes. Quantitative Northern analysis and in situ hybridization have shown that the decidua expresses mRNA for tPA. However, the immunolocalized tPA protein was most strongly associated with the amnion and chorion, as was its receptor annexin II, suggesting that the amnion and chorion are the targets for decidual tPA. At term, decidual tPA expression was unaffected by labor, and the tPA receptor was elevated both before and after labor. At preterm, the converse was found: decidual tPA expression was significantly (p < 0. 05) up-regulated by labor, but the tPA receptor was not. The results suggest that the generation of plasmin at term would be controlled by an increased concentration of the tPA receptor in the amnion and chorion, whereas at preterm a pathological increase in plasmin would be generated by an overexpression of tPA, initiated by labor.  (+info)

Annexin II and bleeding in acute promyelocytic leukemia. (3/431)

BACKGROUND: Acute promyelocytic leukemia (APL) is associated with a hemorrhagic disorder of unknown cause that responds to treatment with all-trans-retinoic acid. METHODS: We studied a newly described receptor for fibrinolytic proteins, annexin II, in cells from patients with APL or other leukemias. We examined initial rates of in vitro generation of plasmin by tissue plasminogen activator (t-PA) in the presence of APL cells that did or did not have the characteristic translocation of APL, t(15;17). We also determined the effect of all-trans-retinoic acid on the expression of annexin II and the generation of cell-surface plasmin. RESULTS: The expression of annexin II, as detected by a fluorescein-tagged antibody, was greater on leukemic cells from patients with APL than on other types of leukemic cells (mean fluorescence intensity, 6.9 and 2.9, respectively; P<0.01). The t(15;17)-positive APL cells stimulated the generation of cell-surface, t-PA-dependent plasmin twice as efficiently as the t(15;17)-negative cells. This increase in plasmin was blocked by an anti-annexin II antibody and was induced by transfection of t(15;17)-negative cells with annexin II complementary DNA. The t(15;17)-positive APL cells contained abundant messenger RNA for annexin II, which disappeared through a transcriptional mechanism after treatment with all-trans-retinoic acid. CONCLUSIONS: Abnormally high levels of expression of annexin II on APL cells increase the production of plasmin, a fibrinolytic protein. Overexpression of annexin II may be a mechanism for the hemorrhagic complications of APL.  (+info)

Localization and quantitation of cardiac annexins II, V, and VI in hypertensive guinea pigs. (4/431)

Annexins are characterized by Ca2+-dependent binding to phospholipids. Annexin II mainly participates in cell-cell adhesion and signal transduction, whereas annexins V and VI also seem to regulate intracellular calcium cycling. Their abundance and localization were determined in left ventricle (LV) and right ventricle (RV) from hypertensive guinea pigs, during the transition from compensatory hypertrophy to heart failure. Immunoblot analysis of annexins II, V, and VI revealed an increased accumulation (2.6-, 1.45-, and 2.3-fold, respectively) in LV from hypertensive guinea pigs and no modification in RV. Immunofluorescent labeling of annexins II, V, and VI; of Na+-K+-ATPase; and of sarcomeric alpha-actinin showed that in control LV and RV, 1) annexin II is present in nonmuscle cells; 2) annexins V and VI are mainly observed in the sarcolemma and intercalated disks of myocytes; 3) annexins II, V, and VI strongly label endothelial cells and adventitia of coronary arteries; and 4) annexin VI is present in the media. At the onset of heart failure, the most striking changes are the increased protein accumulation in LV and the very strong labeling of annexins II, V, and VI in interstitial tissue, suggesting a role in fibrosis development and cardiac remodeling.  (+info)

The biological effects induced in mice by p36, a proteinaceous factor of virulence produced by African swine fever virus, are mediated by interleukin-4 and also to a lesser extent by interleukin-10. (5/431)

We have previously presented indirect evidence that both specific immunosuppression and lymphocyte mitogenicity induced in mice by p36, a proteinaceous factor of virulence produced by porcine monocytes infected by African swine fever virus, were consistent with a Th2-driven response. Here we show: (1) Interleukin-4 (IL-4) and interleukin-10 (IL-10) mRNA expression in the spleen and thymus of C57BL/6 mice were displayed early after p36 inoculation. The expression of thymic IL-10 mRNA occurred, however, later than that of IL-4 mRNA. (2) Increased serum levels of these two cytokines were also soon detected after the protein inoculation. (3) Both immunosuppressive and mitogenic effects of p36 were absent in IL-4 gene-targeted mice and partially abrogated in mice depleted of IL-4 by neutralizing monoclonal antibodies. (4) IL-10 depletion abrogated the immunosuppressive but not the p36 lymphocyte mitogenic biological effects. (5) The increase in the serum concentrations of both IL-4 and IL-10 were lower in thymectomized than in non-thymectomized mice. (6) The expression of interferon-gamma (IFN-gamma) mRNA was weakly or not at all induced in p36-treated mice. Taken together, these results are in agreement with the promotion of a Th2 immune response induced by p36.  (+info)

Dexamethasone alters arachidonate release from human epithelial cells by induction of p11 protein synthesis and inhibition of phospholipase A2 activity. (6/431)

The effect of the glucocorticosteroid, dexamethasone, on arachidonic acid (AA) release and on protein levels of p11 and cytosolic phospholipase A2 (cPLA2) was studied in two epithelial cell lines, HeLa cells and BEAS-2B cells. Dexamethasone treatment of HeLa cells and BEAS-2B cells increased cellular p11 protein and mRNA levels in a time- and dose-dependent manner. It had little effect on levels of cPLA2 protein. In order to determine if increased p11 protein expression resulted in increased interaction between p11 and cPLA2, anti-cPLA2 antibodies were used to immunoprecipitate p11.cPLA2 complexes and Western blots of the immunoprecipitate were used to detect p11. In cells treated with dexamethasone, more p11 was detected in the anti-cPLA2 immunoprecipitate compared with control cells. Dexamethasone treatment of HeLa cells prelabeled with [3H]AA decreased the release of [3H]AA under basal conditions and after stimulation with the calcium ionophore A23187 (10(-6) M). In order to determine if altering the p11 protein levels in HeLa cells independent of glucocorticosteroid treatment could also produce an effect on [3H]AA release, cells were stably transfected with plasmids expressing either p11 antisense mRNA or p11 mRNA. Cloned HeLa cells expressing p11 antisense mRNA exhibited less cellular p11 protein compared with control cells and greater [3H]AA release compared with cells transfected with a control vector. Cloned HeLa cells stably transfected with a p11 expression vector exhibited increased p11 cellular protein and diminished [3H]AA release under basal conditions and in response to A23187. Therefore, dexamethasone alteration of epithelial cell AA release may be due in part to induction of p11 protein expression.  (+info)

Annexin II increases osteoclast formation by stimulating the proliferation of osteoclast precursors in human marrow cultures. (7/431)

Annexin II (AXII), a calcium-dependent phospholipid-binding protein, has been recently found to be an osteoclast (OCL) stimulatory factor that is also secreted by OCLs. In vitro studies showed that AXII induced OCL formation and bone resorption. However, the mechanism of action by which AXII acts as a soluble extracellular protein to induce OCL formation is unknown. In this paper, we demonstrate that AXII gene expression is upregulated by 1,25-dihydroxyvitamin D3 [1, 25-(OH)2D3] and that addition of AXII significantly increased OCL-like multinucleated cell formation. Time-course studies suggested that AXII acted on the proliferative stage of OCL precursors and that AXII increased thymidine incorporation in OCL precursors. Moreover, AXII enhanced the growth of CFU-GM, the earliest identifiable OCL precursor, when bone marrow cultures were treated with low concentrations of GM-CSF. This capacity of AXII to induce OCL precursor proliferation was due to induction of GM-CSF expression, because the addition of neutralizing antibodies to GM-CSF blocked the stimulatory effect of AXII on OCL formation. RT-PCR analysis using RNA from highly purified subpopulations of marrow cells demonstrated that T cells, especially CD4(+) T cells, produced GM-CSF in response to AXII. Furthermore, FACS(R) analysis of T-cell subpopulations treated with fluorescein-labeled AXII suggested that the CD4(+), but not CD8(+), subpopulation of T cells express an AXII receptor. Taken together, these data suggest that AXII stimulates OCL formation by activating T cells through a putative receptor to secrete GM-CSF. GM-CSF then expands the OCL precursor pool to enhance OCL formation.  (+info)

Interference with annexin II has no effect on entry of human cytomegalovirus into fibroblast cells. (8/431)

Annexin II has been identified as a human cytomegalovirus (HCMV)-binding protein, shown to be a component of purified virions and proposed as a cellular receptor for the virus. In addition, annexin II is capable of associating with the major HCMV envelope glycoprotein, gB (gpUL55). As one approach to examine the role of annexin II in virus entry, a high-titre polyclonal annexin II-specific antibody was produced and its effects in virus entry and cell-to-cell spread assays were tested. This anti-annexin II serum recognized virion and cell surface annexin II and annexin II-derived peptides. Recombinant annexin II, with characteristic calcium- and phospholipid-binding activities, was also examined. Pretreatment of cells, virions or both with polyclonal anti-annexin II serum, affinity-purified anti-annexin II antibodies or recombinant annexin II protein prior to infection was inconsequential to the entry of HCMV into fibroblasts. HCMV was also able to dose-dependently penetrate annexin II-deficient 293 cells. Furthermore, the spread of HCMV from cell to cell was not inhibited in the presence of polyclonal anti-annexin II antibodies or exogenous annexin II protein. These experiments do not support a direct role of annexin II in virus entry or spread. Alternative roles for the gB-annexin II interaction are proposed.  (+info)

C57BL/6 mice were immunized intraperitoneally twice with 100 nmol of I-Ab-restricted MOG35-55 peptide (MEVGWYRSPFSRVVHLYRNGK, MBL code no. TS-M704-P) and 10 µg cholera toxin (MBL code no. RK-01-511) in complete Freunds adjuvant. Eleven days later, splenocytes were isolated and stained with I-Ab MOG35-55 tetramer-PE (MBL code no. TS-M704-1; Day 0). An aliquot of splenocytes was stimulated with 10µg/mL MOG35-55 peptide for 6 days in vitro and then stained with MHC class II tetramer (Day 6). in vitro stimulation of with I-Ab MOG35-55 resulted in increased tetramer-positive CD4+ T cells from immunized (mouse 1 and 2), but not naïve (mouse 3), mice.. ...
Annexin II antibody (annexin A2) for ICC/IF, IHC-P, IP, RipA, WB. Anti-Annexin II pAb (GTX32446) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
Annexin A2 is a calcium-dependent, phospholipid-binding protein found on many cell types. It consists of a short hydrophobic tail (Ser2-Asn32), which dictates its function, and a core domain (Phe33-Asp339), which is involved in phospholipid binding. Annexin A2 has been implicated in a number of biochemical processes, including cell proliferation, foetal immune tolerance, ion-channel activation, cell-cell interactions and the bridging of membranes. Annexin A2 is reported to be a powerful activator of plasminogen and, therefore, is implicated in many normal and pathological processes such as haemostasis and metastasis. Myeloid cell lines are used, extensively, to study many aspects of cellular proliferation, differentiation and function. In the present study, we have used flow cytometry and real-time PCR to investigate the role of annexin A2 expression in the proliferation and differentiation of a number of myeloid cell lines. The results demonstrated that annexin A2 expression was affected when ...
Purpose: : To examine the role of annexin 2 in the formation and internalisation of phagosomes in retinal pigment epithelial (RPE) cells. Methods: : Human ARPE19 cells were cultured on the glass inserts of Matek dishes for immunofluorescence and live imaging, or plastic Nunc dishes for biochemical analysis. In some studies ARPE19 cells were exposed to siRNA for annexin 2 for three days prior to experimentation. Outer segments were prepared from porcine retina using standard protocols (typically 50 - 100 eyes per prep), and either used fresh in biochemical experiments, or labelled with an appropriate fluorescent marker prior to imaging experiments. Results: : Depletion of annexin 2 using siRNA led to a significant quantitative reduction in the phagocytosis of rod outer segments in cultured RPE cells. In cells expressing annexin 2, imaged by confocal microscopy, a striking enrichment of annexin 2 and F-actin was observed at the phagocytic cup immediately upon engagement of the apical cell surface ...
In our hands, the trypsin actually reduced the Annexin staining presumably by stripping off all the phosphotidyl-serine on the surface along with the surface proteins (adhesion molecules). The only time we have seen enhanced annexin is when the cells suffer enough trauma in harvesting that the membrane is not intact and the Annexin gets inside the cell and attaches to the ps on the inner membrane. Julie At 02:11 PM 8/18/98, you wrote: , ,I have a client who is staining with Annexin-FITC. They are looking at ,cell lines and are using trypsin to get the cells off the flasks. We are ,seeing what we think is an exagerated Annexin response. Does anyone out ,there have any feedback on how trypsin affects Annexin staining? Any ,experience and or information about this will be greatly appreciated. , Thanks in advance, , Joan Kalnitsky ,Joan Kalnitsky ,Flow Cytometry Laboratory ,VMRCVM ,(540) 231-4115 ,FAX 540-231-7367 , , It is better to serve than to receive , B. Borg , , , , , ...
Low Ag dose promotes induction and persistence of regulatory T cells (Tregs) in mice, yet few studies have addressed the role of Ag dose in the induction of adaptive CD4+FOXP3+ Tregs in humans. To this end, we examined the level of FOXP3 expression in human CD4+CD25− T cells upon activation with autologous APCs and varying doses of peptide. Ag-specific T cells expressing FOXP3 were identified by flow cytometry using MHC class II tetramer (Tmr). We found an inverse relationship between Ag dose and the frequency of FOXP3+ cells for both foreign Ag-specific and self Ag-specific T cells. Through studies of FOXP3 locus demethylation and helios expression, we determined that variation in the frequency of Tmr+FOXP3+ T cells was not due to expansion of natural Tregs, but instead, we found that induction, proliferation, and persistence of FOXP3+ cells was similar in high- and low-dose cultures, whereas proliferation of FOXP3− T cells was favored in high Ag dose cultures. The frequency of FOXP3+ cells ...
Cellular immunity plays an important role in the control of persistent virus infections such as hepatitis C virus (HCV). Antiviral CD4(+) T cell responses have been shown to accompany resolution of acute disease and there is also a consistent association between HLA Class II genes, notably HLADRB1*1101 (and the closely linked HLADQB1*0301) and disease resolution. We initially mapped longitudinal CD4(+) T cell responses in an individual after spontaneous resolution of acute HCV, and identified three HLA-DR11-restricted responses which vary in immunodominance over time. Functional assays and HLA Class II tetramer staining revealed one to be a response to a commonly recognized epitope, NS3(1248-1261), although cytokine capture assays showed these specific cells to be at a very low frequency. In this patient, and in others reported, this most frequently recognized HLA-DR11 restricted epitope is not immunodominant. We analysed whether sequence variability within and between genotypes might account for
The Annexin family of calcium-binding proteins is composed of al least thirteen mammalian genes (Annexin A1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. The Annexin family
Order monoclonal and polyclonal Annexin A13 antibodies for many applications. Selected quality suppliers for anti-Annexin A13 antibodies.
Annexin A1兔多克隆抗体(ab65844)可与小鼠, 大鼠, 人样本反应并经WB, IP, IHC, ICC实验严格验证。所有产品均提供质保服务,中国75%以上现货。
Annexin A2兔多克隆抗体(ab90643)可与人样本反应并经WB, IHC实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
Abcam provides specific protocols for Anti-Annexin A10 antibody (ab2343) : Western blot protocols, Immunohistochemistry protocols
ANXA4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 321 amino acids (1-321 a.a.) and having a molecular mass of 36 kDa.
Product Pig Annexin A8(ANXA8) ELISA kit From B-Gene - A competitive ELISA for quantitative measurement of Porcine Annexin A8(ANXA8) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUTION*1 vial 12. WASH SOLUTION (100 x)*1 vial 13. BALANCE SOLUTION*1 vial 14. INSTRUCTION*1
This graph shows the total number of publications written about Annexin A3 by people in this website by year, and whether Annexin A3 was a major or minor topic of these publications ...
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Rabbit recombinant monoclonal Annexin A10 antibody [EPR13101] validated for WB and tested in Human, Mouse and Rat. Immunogen corresponding to recombinant…
BioAssay record AID 502910 submitted by ChEMBL: Induction of annexin 2-mediated cytoskeleton aggregate accumulation in human HepG2 cells at 4 uM after 2 hrs by confocal laser scanning microscopy.
Annexin A11 Antibody 10479-2-AP has been identified with IF, IHC, IP, WB, ELISA. 10479-2-AP detected 56-60 kDa band in HeLa cells with 1:500-1:2000 dilution...
Bachem offers H-3196 Annexin A1 (1-25) (dephosphorylated) (human) for your research. Find all specific details here. Find product specific information including available pack sizes, CAS, detailed description and references here.
Mouse Monoclonal Anti-Annexin A3 Antibody (2F3). Validated: WB, ICC/IF, IHC, IHC-P. Tested Reactivity: Human, Monkey. 100% Guaranteed.
Because of its Ca2+-regulated actin and membrane binding properties, annexin 2 is a probable candidate for participating in the regulation of actin assembly and/or the formation of membrane-cytoskeleton links at certain membrane sites. This is supported by several reports revealing a relationship between annexin 2 and the actin cytoskeleton that underlies cellular membranes (Babiychuk and Draeger, 2000; Harder and Gerke, 1993; Harder and Gerke, 1994; Merrifield et al., 2001; Oliferenko et al., 1999; Zobiack et al., 2002). Recently, annexin 2 was found to be specifically enriched at the membrane-F-actin interface of motile pinosomes (Merrifield et al., 2001). The formation of macropinosome actin tails was inhibited by the overexpression of a trans-dominant annexin 2 mutant protein, which revealed an essential role for annexin 2 in the generation of these structures. However, the finding that annexin 2 is not recruited to actin tails assembled behind moving Listeria, shows that annexin 2 is not ...
Annexin A1 is an intracellular calcium/phospholipid-binding protein that is involved in membrane organization and the regulation of the immune system. It has been attributed an anti-inflammatory role at various control levels, and recently we could show that annexin A1 externalization during secondary necrosis provides an important fail-safe mechanism counteracting inflammatory responses when the timely clearance of apoptotic cells has failed. As such, annexin A1 promotes the engulfment of dying cells and dampens the postphagocytic production of proinflammatory cytokines. In our current follow-up study, we report that exposure of annexin A1 during secondary necrosis coincided with proteolytic processing within its unique N-terminal domain by ADAM10. Most importantly, we demonstrate that the released peptide and culture supernatants of secondary necrotic, annexin A1-externalizing cells induced chemoattraction of monocytes, which was clearly reduced in annexin A1- or ADAM10-knockdown cells. Thus, ...
Early in the apoptosis process, phosphatidylserine (PS) becomes exposed on the cell surface by flipping from the inner to outer leaflet of the cytoplasmic membrane. This event is thought to be important for macrophage recognition of cells undergoing apoptosis, thus allowing the cells to be rapidly phagocytosed. The binding of Annexin V to phosphatidylserine is calcium-dependent, reversible, but very tight, with a Kd of approximately 5 x 10-10 M. At low PS concentration, a binding ratio of 8 annexins to one PS has been reported. These features make Annexin V conjugates ideal for identifying membrane changes associated with apoptosis, by using either flow cytometric or in situ labeling methods. TACS® Annexin V kits are supplied with an Annexin V conjugate, an optimized binding buffer and propidium iodide. Cells are harvested and washed, then incubated with an Annexin V conjugate in binding buffer, for 15 minutes at room temperature prior to direct analysis. Alternatively, a secondary streptavidin ...
Membrane repair is essential to cell survival. In skeletal muscle, injury often associates with plasma membrane disruption. Additionally, muscular dystrophy is linked to mutations in genes that produce fragile membranes or reduce membrane repair. Methods to enhance repair and reduce susceptibility to injury could benefit muscle in both acute and chronic injury settings. Annexins are a family of membrane-associated Ca2+-binding proteins implicated in repair, and annexin A6 was previously identified as a genetic modifier of muscle injury and disease. Annexin A6 forms the repair cap over the site of membrane disruption. To elucidate how annexins facilitate repair, we visualized annexin cap formation during injury. We found that annexin cap size positively correlated with increasing Ca2+ concentrations. We also found that annexin overexpression promoted external blebs enriched in Ca2+ and correlated with a reduction of intracellular Ca2+ at the injury site. Annexin A6 overexpression reduced membrane ...
Annexin V Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidyl-serine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. The one-step staining procedure takes only 10 minutes. Detection can be analyzed by flow cytometry or by fluorescence microscopy. |p>|br />|br />‧ Detection method- Flow cytometry (Ex = 543 nm; Em = 570 nm) and fluorescence microscopy |br>|br />‧ Sample type- Living cells (suspension and adherant)|br>|br />‧ Species reactivity- Mammalian |br />|br />|br />|p>|b>Features and Benefits|/b>|br>|br />‧ Simple one step staining procedure in 10 minutes|br>|br />‧ Fast and convenient|br>|br />‧ Cy3 shows brighter red fluorescence|br />|br />|br />|br />|p>|b>Kit Contents:|/b>|br>|br />Annexin V-Cy3|br>|br />1X Binding Buffer, Annexin V
The pHi (intracellular pH) is an important physiological parameter which is altered during hypoxia and ischaemia, pathological conditions accompanied by a dramatic decrease in pHi. Sensors of pHi include ion transport systems which control intracellular Ca2+ gradients and link changes in pHi to functions as diverse as proliferation and apoptosis. The annexins are a protein family characterized by Ca2+-dependent interactions with cellular membranes. Additionally, in vitro evidence points to the existence of pH-dependent, Ca2+-independent membrane association of several annexins. We show that hypoxia promotes the interaction of the recombinant annexin A2-S100A10 (p11) and annexin A6 with the plasma membrane. We have investigated in vivo the influence of the pHi on the membrane association of human annexins A1, A2, A4, A5 and A6 tagged with fluorescent proteins, and characterized this interaction for endogenous annexins present in smooth muscle and HEK (human embryonic kidney)-293 cells ...
TY - JOUR. T1 - Annexin A2. T2 - Its molecular regulation and cellular expression in cancer development. AU - Wang, Chi Yun. AU - Lin, Chiou Feng. PY - 2014. Y1 - 2014. N2 - Annexin A2 (ANXA2) orchestrates multiple biologic processes and clinical associations, especially in cancer progression. The structure of ANXA2 affects its cellular localization and function. However, posttranslational modification and protease-mediated N-terminal cleavage also play critical roles in regulating ANXA2. ANXA2 expression levels vary among different types of cancers. With some cancers, ANXA2 can be used for the detection and diagnosis of cancer and for monitoring cancer progression. ANXA2 is also required for drug-resistance. This review discusses the feasibility of ANXA2 which is active in cancer development and can be a therapeutic target in cancer management.. AB - Annexin A2 (ANXA2) orchestrates multiple biologic processes and clinical associations, especially in cancer progression. The structure of ANXA2 ...
|p|Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry
Invitrogen™ eBioscience™ Annexin V, Recombinant Protein Unlabeled; 300μg Invitrogen™ eBioscience™ Annexin V, Recombinant Protein...
Annexin V Binding Buffer - Annexin V Binding Buffer has been formulated for flow cytometric labeling of apoptotic cells with Annexin V reagents.  The buffer is provided as a ready-to-use solution..
ELISA method for the measurement of IgM isotype auto antibodies targeted to annexin V. Assay designed with highly purified human recombinant annexin V, obtained with non-denaturing methods, for auto antibody immunocapture. These are then revealed with a goat antibody specific for IgM, micro fragments, and labeled with peroxidase.
We examined sera and placenta tissue from 156 patients in reproductive age with unexplained repeated pregnancy loss. Control group of eighty-four fertile healthy women was included. ELISA methods were used for detecting a panel of sera of anti-phospholipid antibodies (aPLs). Immunolocalization of annexin V receptors in 143 trophoblast-placenta specimen of 156 patients was investigated by the immunofluorescence technique using Annexin V-FITC, Apoptosis and Annexin V-CY3 commercial kits ...
In the present study, we show that both CRP and annexin A5 specifically bind to oxidized LDL. Via coupling to Fcγ receptors on macrophages and independent of complement, CRP subsequently enhances the association of oxidized LDL to macrophages. Enhanced association of oxidized LDL to macrophages via CRP-Fcγ receptor interaction may lead to enhanced macrophage uptake of oxidized LDL. Moreover, we show that annexin A5 binds to a site at oxidized LDL different from the CRP binding site and that annexin A5 does not antagonize the CRP effect.. Previously, Chang et al also observed specific binding of CRP to oxidized LDL,7 characterized by inhibition by whole molecules as well as F(ab′)2 fragments of EO6, an IgM antibody that binds specifically to the phosphorylcholine head group of oxidized phospholipids but not to the same moiety on nonoxidized phospholipids.26 In the present study, for characterization of binding sites, we used the monoclonal antibody DLH3 (also IgM), which recognizes an epitope ...
Annexin 1 is an anti-inflammatory protein that plays a key role in innate immunity by modulating the activation of several types of cells, including neutrophils. Here we have developed a cleavage assay using tagged annexin 1 and observed marked activity in the membrane fraction of activated neutrophils. A combination of inhibitors, transfected cells, and proteomic analyses allowed us to identify proteinase 3 as the main enzyme responsible for this cleavage in the N terminus region of the protein, at least in the context of neutrophil activation. Because annexin 1 is an important endogenous anti-inflammatory mediator, blocking its cleavage by proteinase 3 would augment its homeostatic pro-resolving actions and could represent an opportunity for innovative anti-inflammatory drug discovery.. ...
TransDetect® Annexin V-EGFP/PI Cell Apoptosis Detection Kit,Cell Detection,Cell Culture and Detection,Products,Beijing TransGen Biotech Co.Ltd,OverviewContents& storageCitations & referencesRelated ImagesDownloadOverviewDescriptionThe Annexin V
TY - JOUR. T1 - Ceramide 1-phosphate mediates endothelial cell invasion via the annexin a2-p11 heterotetrameric protein complex. AU - Hankins, Jody L.. AU - Ward, Katherine E.. AU - Linton, Sam S.. AU - Barth, Brian M.. AU - Stahelin, Robert V.. AU - Fox, Todd E.. AU - Kester, Mark. PY - 2013/7/5. Y1 - 2013/7/5. N2 - The bioactive sphingolipid, ceramide 1-phosphate (C-1-P), has been implicated as an extracellular chemotactic agent directing cellular migration in hematopoietic stem/progenitor cells and macrophages. However, interacting proteins that could mediate these actions of C-1-P have, thus far, eluded identification. We have now identified and characterized interactions between ceramide 1-phosphate and the annexin a2-p11 heterotetramer constituents. This C-1-P-receptor complex is capable of facilitating cellular invasion. Herein, we demonstrate in both coronary artery macrovascular endothelial cells and retinal microvascular endothelial cells that C-1-P induces invasion through an ...
ANXA5 Mouse Recombinant produced in E. coli is a single, non-glycosylated polypeptide chain containing 342 amino acids (1-319 a.a) and having a molecular mass of 38.1kDa.
Animals. Wild-type mice from the 129T2/SvEmsJ background were bred and housed in a specific pathogen-free facility on a 12-hour light/12-hour dark cycle and fed ad libitum in accordance with the Northwestern Universitys Institutional Animal Care and Use Committee regulations. 129T2/SvEmsJ (129T2) mice were purchased from the Jackson Laboratory (stock 002065). mdx/hLTBP4 mice were generated as described previously (34, 60). Sgcg-null mice were generated as described in Hack et al. (46). Two- to 3-month-old males and females were used for all wild-type mouse experiments. Sgcg-null cohorts were age and sex matched with mice between 2 and 5 months old.. Plasmids. Plasmids encoding annexin A1, A2, and A6 with a carboxyl-terminal turboGFP tag were obtained from Origene. Subcloning of annexin A1, A2, and A6 to replace the GFP tag with tdTomato (Addgene) was performed by Mutagenix. Site-directed mutagenesis was performed by Mutagenix on annexin A1-GFP, A2-GFP, and A6-GFP to create the Ca2+-binding ...
Animals. Wild-type mice from the 129T2/SvEmsJ background were bred and housed in a specific pathogen-free facility on a 12-hour light/12-hour dark cycle and fed ad libitum in accordance with the Northwestern Universitys Institutional Animal Care and Use Committee regulations. 129T2/SvEmsJ (129T2) mice were purchased from the Jackson Laboratory (stock 002065). mdx/hLTBP4 mice were generated as described previously (34, 60). Sgcg-null mice were generated as described in Hack et al. (46). Two- to 3-month-old males and females were used for all wild-type mouse experiments. Sgcg-null cohorts were age and sex matched with mice between 2 and 5 months old.. Plasmids. Plasmids encoding annexin A1, A2, and A6 with a carboxyl-terminal turboGFP tag were obtained from Origene. Subcloning of annexin A1, A2, and A6 to replace the GFP tag with tdTomato (Addgene) was performed by Mutagenix. Site-directed mutagenesis was performed by Mutagenix on annexin A1-GFP, A2-GFP, and A6-GFP to create the Ca2+-binding ...
Animals. Wild-type mice from the 129T2/SvEmsJ background were bred and housed in a specific pathogen-free facility on a 12-hour light/12-hour dark cycle and fed ad libitum in accordance with the Northwestern Universitys Institutional Animal Care and Use Committee regulations. 129T2/SvEmsJ (129T2) mice were purchased from the Jackson Laboratory (stock 002065). mdx/hLTBP4 mice were generated as described previously (34, 60). Sgcg-null mice were generated as described in Hack et al. (46). Two- to 3-month-old males and females were used for all wild-type mouse experiments. Sgcg-null cohorts were age and sex matched with mice between 2 and 5 months old.. Plasmids. Plasmids encoding annexin A1, A2, and A6 with a carboxyl-terminal turboGFP tag were obtained from Origene. Subcloning of annexin A1, A2, and A6 to replace the GFP tag with tdTomato (Addgene) was performed by Mutagenix. Site-directed mutagenesis was performed by Mutagenix on annexin A1-GFP, A2-GFP, and A6-GFP to create the Ca2+-binding ...
Annexin V is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Annexin V is a monomer, binds ATRX and EIF5B, and interacts with hepatitis B virus (HBV ...
SCAN HISTORY OWL11_0 2 100 NSINGLE OWL17_1 2 110 NSINGLE OWL18_0 1 115 NSINGLE OWL19_1 1 125 NSINGLE OWL21_1 1 125 NSINGLE OWL26_0 1 125 NSINGLE SPTR37_9f 2 237 NSINGLE INITIAL MOTIF SETS ANNEXIN1 Length of motif = 23 Motif number = 1 Annexin motif I - 1 PCODE ST INT KTKGVDEVTIVNILTNRSNAQRQ LUHU36 46 46 KTKGVDEVTIINILTNRSNEQRQ ANX2_CHICK 46 46 TVKGVDEATIIDILTKRNNAQRQ ANX1_CAVCU 56 56 MVKGVDEATIIDILTKRNNAQRQ LUHU 55 55 KGIGTDEATIIDIVTHRSNAQRQ ANX6_MOUSE 376 376 KGMGTDEETILKILTSRNNAQRQ ANX5_CHICK 29 29 KGIGTNEQAIIDVLTKRSNTQRQ ANX8_HUMAN 35 35 KGFGTDEQEIIDVLVGRSNQQRQ DROANNX 29 29 RGIGTDEKMLISILTERSNAQRQ ANX3_HUMAN 32 32 KGLGTDEDAIINVLAYRSTAQRQ ANX4_BOVIN 27 27 KGFGTDEQAIVDVVANRSNDQRQ HUMSNEXIN 177 177 TAKGVDEATIIDIMTTRTNAQRP ANX1_COLLI 51 51 ANNEXIN2 Length of motif = 17 Motif number = 2 Annexin motif II - 1 PCODE ST INT LKSALSGHLETVILGLL LUHU36 86 17 LKSALSGHLEAVILGLL ANX2_CHICK 86 17 LKKALTGHLEEVVLALL ANX1_CAVCU 96 17 LKKALTGHLEEVVLALL LUHU 95 17 LKSEISGDLARLILGLM ANX6_MOUSE 416 17 ...
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1HVD: Structural and electrophysiological analysis of annexin V mutants. Mutagenesis of human annexin V, an in vitro voltage-gated calcium channel, provides information about the structural features of the ion pathway, the voltage sensor and the ion selectivity filter
Complete information for ANXA1 gene (Protein Coding), Annexin A1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Complete information for ANXA1 gene (Protein Coding), Annexin A1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Annexin A2 is a calcium regulated protein involved in membrane binding and actin polymerization. In this paper, Kevin Moreau and colleagues identified Annexin A2 as a…. ...
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E1 beta subunit of the complex forming an heterotetramer alpha2beta2 bound to two TPP (thiamine-pyrophosphate) one each bridging E1 alpha and E1 beta to form an active site catalyzing the irreversible decarboxylation of pyruvate to acetyl- ...
Morel, Etienne; Gruenberg, Jean (2007-10-31). "The p11/S100A10 Light Chain of Annexin A2 Is Dispensable for Annexin A2 ... Annexin A2 (previously named Annexin II), the vacuolar ATPase, COP coat proteins, the N-ethylmaleimide sensitive factor, the ... Morel, Etienne; Gruenberg, Jean (2009-01-16). "Annexin A2 binding to endosomes and functions in endosomal transport are ... Morel, Etienne; Parton, Robert G.; Gruenberg, Jean (2009). "Annexin A2-dependent polymerization of actin mediates endosome ...
... has been shown to interact with PTPN11 and Annexin A2. Carcinoembryonic antigen Cluster of differentiation GRCh38: ... Kirshner J, Schumann D, Shively JE (December 2003). "CEACAM1, a cell-cell adhesion molecule, directly associates with annexin ...
Annexin A2, a membrane-bound protein, helps regulate vomocytosis and promote the fusing of vesicles to the plasma membranes. In ... annexin A2 deficient cell lines, rates of vomocytosis were decreased. Furthermore, screens of macrophage kinase inhibitors ...
One candidate is Annexin A2, identified as being able of binding progastrin and derived peptides. Annexin A2 is a partial ... However, progastrin affinity for Annexin A2 is not what would be expected for a specific receptor. And, although Annexin A2 ... In particular, Annexin A2 mediates NF-kappaB upregulation and ß-catenin in response to progastrin in mice and HEK-293 cells. In ... Sarkar, Shubhashish; Swiercz, Rafal; Kantara, Carla; Hajjar, Katherine A.; Singh, Pomila (2011). "Annexin A2 Mediates Up- ...
Annexin A2, an endogenous protein, is a natural inhibitor of PCSK9 activity. GRCh38: Ensembl release 89: ENSG00000169174 - ... Mayer G, Poirier S, Seidah NG (November 2008). "Annexin A2 is a C-terminal PCSK9-binding protein that regulates endogenous low ... "Annexin A2 is a natural extrahepatic inhibitor of the PCSK9-induced LDL receptor degradation". PLOS ONE. 7 (7): e41865. Bibcode ...
He KL, Deora AB, Xiong H, Ling Q, Weksler BB, Niesvizky R, Hajjar KA (July 2008). "Endothelial cell annexin A2 regulates ...
Additionally, it has been shown that SLPI is able to bind the Annexin A2/S100A10 heterotetramer (A2t), a co-factor HIV ... "The S100A10 subunit of the annexin A2 heterotetramer facilitates L2-mediated human papillomavirus infection". PLOS ONE. 7 (8): ... "Secretory leukocyte protease inhibitor binds to annexin II, a cofactor for macrophage HIV-1 infection". The Journal of ...
2012). "The S100A10 subunit of the annexin A2 heterotetramer facilitates L2-mediated human papillomavirus infection". PLOS ONE ... and annexin A2 leading to entry of the virions into basal epithelial cells through clathrin-mediated endocytosis and/or ...
Lennon NJ, Kho A, Bacskai BJ, Perlmutter SL, Hyman BT, Brown RH (2003). "Dysferlin interacts with annexins A1 and A2 and ... including annexin and MG53. Exactly how dysferlin contributes to membrane resealing is not clear, but biochemical evidence ... "Enhanced Muscular Dystrophy from Loss of Dysferlin Is Accompanied by Impaired Annexin A6 Translocation after Sarcolemmal ...
Knop M, Aareskjold E, Bode G, Gerke V (2005). "Rab3D and annexin A2 play a role in regulated secretion of vWF, but not tPA, ...
"Prohibitin and prohibitone are contained in high-molecular weight complexes and interact with alpha-actinin and annexin A2". ...
Goel M, Sienkiewicz AE, Picciani R, Lee RK, Bhattacharya SK (2011). "Cochlin induced TREK-1 co-expression and annexin A2 ...
Specifically, HPV16 entry into Langerhans cells via the annexin A2/S100A10 heterotetramer results in suppressive signaling and ... a novel role for the annexin A2 heterotetramer in immune suppression". Journal of Immunology. 192 (10): 4748-4757. doi:10.4049/ ...
Kwon M, MacLeod TJ, Zhang Y, Waisman DM (Jan 2005). "S100A10, annexin A2, and annexin a2 heterotetramer as candidate ... He KL, Deora AB, Xiong H, Ling Q, Weksler BB, Niesvizky R, Hajjar KA (Jul 2008). "Endothelial cell annexin A2 regulates ... Annexin II, which is attracted to negatively charged phospholipids, binds to p11 at the Ca2+ binding site. In addition, Annexin ... "The S100A10 subunit of the annexin A2 heterotetramer facilitates L2-mediated human papillomavirus infection". PLOS ONE. 7 (8): ...
With further research and development, Jaffee has hopes to make the protein, Annexin A2, not as overworked to prevent the ... Annexina2 as Immunological Target, and Prostate Stem Cell Antigen Vaccines and Uses Thereof. In 2007, Dr. Elizabeth Jaffee co- ...
... annexin a1 MeSH D12.776.157.125.050.060 - annexin a2 MeSH D12.776.157.125.050.070 - Annexin A3 MeSH D12.776.157.125.050.080 - ... annexin a4 MeSH D12.776.157.125.050.100 - annexin a5 MeSH D12.776.157.125.050.110 - annexin a6 MeSH D12.776.157.125.050.120 - ... annexin a7 MeSH D12.776.157.125.412.249 - calmodulin MeSH D12.776.157.125.412.311 - calnexin MeSH D12.776.157.125.412.374 - ...
Annexin A2, CAF-1, CDC25C, CHTF18, Cyclin D1, Cyclin O, Cyclin-dependent kinase 4, Cyclin-dependent kinase inhibitor 1C, DNMT1 ...
Cytogenetic band: 4q27 TMEM155 is neighbored by TMEM155 is neighbored on chromosome 4 by CCNA2, a gene encoding for cyclin A2, ... and ANXA5, which encodes annexin A5. It is also neighbored by PP12613 located on the positive strand. The gene on chromosome 4 ...
... s are found as calcium-binding features in annexins, matrix metalloproteinases (e.g.serralysins), subtilisins and ... phospholipase A2. They are also observed in synthetic peptides and in cyclic hexapeptides made from alternating D,L amino acids ...
However some annexins (Annexin A1, Annexin A2, and Annexin A5) have also been found outside the cellular environment, for ... Annexin, type I InterPro: IPR002388 Annexin, type II InterPro: IPR002389 Annexin, type III InterPro: IPR002390 Annexin, type IV ... In addition to annexin A-II, annexin A-XI has also been shown to organize cell membrane properties. Annexin A-XI is believed to ... The 310 amino acid annexin core has four annexin repeats, each composed of 5 alpha-helices. The exception is annexin A-VI that ...
... a protein that in humans is encoded by the EDA2R gene Alpha-2 adrenergic receptor Annexin A2, a protein Apolipoprotein A2, a ... A2, A02, A002, A², A.II or A-2 may refer to: British NVC community A2 (Lemna minor community), a plant community A2, the second ... A2, an Italian Baseball League A2, an Italian Volleyball League A2, a grade in climbing A2 (remote television production), a ... an enzyme Hemoglobin A2, a protein Proanthocyanidin A2, an A type proanthocyanidin Urea transporter A2, a protein A2, a variant ...
... also known as annexin II is a protein that in humans is encoded by the ANXA2 gene. Annexin 2 is involved in diverse ... Kwon M, MacLeod TJ, Zhang Y, Waisman DM (Jan 2005). "S100A10, annexin A2, and annexin a2 heterotetramer as candidate ... "Entrez Gene: ANXA2 annexin A2". Kling T, Ferrarese R, Ó hAilín D, Johansson P, Heiland DH, Dai F, Vasilikos I, Weyerbrock A, ... Annexin A2 has been proposed to function inside the cell in sorting of endosomes and outside the cell in anticoagulant ...
"Sedimentation equilibrium analysis of five lipocortin-related phospholipase A2 inhibitors from human placenta. Evidence against ... Annexin A5 (or annexin V) is a cellular protein in the annexin group. In flow cytometry, annexin V is commonly used to detect ... The annexin A5 affinity assay typically uses a conjugate of annexin V and a fluorescent or enzymatic label, biotin or other ... Annexin A5 showed upregulation in papillary thyroid carcinoma. Annexin A5 is used as a non-quantitative probe to detect cells ...
... protein has an apparent relative molecular mass of 40 kDa with phospholipase A2 inhibitory activity. Glucocorticoids ... Annexin A1, also known as lipocortin I, is a protein that is encoded by the ANXA1 gene in humans. Annexin A1 belongs to the ... Annexin A1 both suppresses phospholipase A2, thereby blocking eicosanoid production, and inhibits various leukocyte ... Annexin A1 has also been shown to be associated with treatment resistance. ARID1A loss activates annexin A1 expression, which ...
This protein functions in the inhibition of phospholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1- ... "The high-resolution crystal structure of human annexin III shows subtle differences with annexin V.". Biochemistry. 35 (6): ... Annexin A3 is a protein that in humans is encoded by the ANXA3 gene. It is abnormally expressed in fetuses of both IVF and ICSI ... "Entrez Gene: ANXA3 annexin A3". Zhang Y, Zhang YL, Feng C, et al. (September 2008). "Comparative proteomic analysis of human ...
... annexin-1) synthesis. Lipocortin-1 both suppresses phospholipase A2, thereby blocking eicosanoid production, and inhibits ... They inhibit prostaglandin synthesis at the level of phospholipase A2 as well as at the level of cyclooxygenase/PGE isomerase ( ... "Glucocorticoids inhibit prostaglandin synthesis not only at the level of phospholipase A2 but also at the level of cyclo- ...
They induce the lipocortin-1 (annexin-1) synthesis, which then binds to cell membranes preventing the phospholipase A2 from ...
... and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in ... Annexin A4 is a protein that in humans is encoded by the ANXA4 gene. Annexin IV (ANX4) belongs to the annexin family of calcium ... "Entrez Gene: ANXA4 annexin A4". Human ANXA4 genome location and ANXA4 gene details page in the UCSC Genome Browser. Römisch J, ... Dreier R, Schmid KW, Gerke V, Riehemann K (Aug 1998). "Differential expression of annexins I, II and IV in human tissues: an ...
Annexin A8-like protein 2 is a protein that in humans is encoded by the ANXA8L2 gene. This gene encodes a member of the annexin ... a novel human Ca2+/phospholipid binding protein that inhibits coagulation and phospholipase A2 activity. Its molecular cloning ... "Entrez Gene: ANXA8 annexin A8". CS1 maint: discouraged parameter (link) Human ANXA8 genome location and ANXA8 gene details page ... 2006). "Annexin A8 is up-regulated during mouse mammary gland involution and predicts poor survival in breast cancer". Clin. ...
Examples of glucocorticoid-responsive genes include those that encode annexin A1, TSC22D3 (also known as GILZ), angiotensin- ... These target genes encode proteins such as cyclooxygenase, NO synthase, phospholipase A2, tumor necrosis factor, transforming ...
Annexin *A1. *A2. *A5. *Fibulin *FBLN1. *FBLN2. *FBLN5. *Vitamin D-dependent calcium-binding protein/Calbindin ...
... and annexin A2[93] leading to entry of the virions into basal epithelial cells through clathrin-mediated endocytosis and/or ... "The S100A10 subunit of the annexin A2 heterotetramer facilitates L2-mediated human papillomavirus infection". PLoS One. 7 (8): ...
... a human gene Annexin A3, a human gene ATC code A03 Drugs for functional gastrointestinal disorders, a subgroup of the ... a document given to employees of A-1 and A-2 Visa Holders who are representing a foreign government inside the U.S. A3, the ...
... annexin A1 (an inhibitor of formation of pro-inflammatory metabolites of polyunsaturated fatty acids), and the gaseous ... 6-epoxyisoprostane A2. Both PGJ2's are arachidonic acid-derived metabolites made by cyclooxygenases, primarily COX-2, which is ...
"Pfam entry: Annexin". Archived from the original on 2007-09-29. Retrieved 2007-01-25. "Pfam entry Synapsin N". Archived from ... "Pfam entry: Phospholipase A2". Archived from the original on 2007-09-29. Retrieved 2007-01-25. "Pfam entry: ... The formation of ionic bridges is seen in the protein-lipid interaction between both protein C2 type domains and annexins.. Any ... They may also occur in BcL-2 like protein , in some amphiphilic antimicrobial peptides , and in certain annexins . These ...
As an example, annexin I is externalized in the same locations as phosphatidylserine and helps with clustering phagocytic ... During apoptosis, caspase 3 activates the Ca2+-independent phospholipase A2, leading to release of lysophosphatidylcholine ...
Annexin A2 also known as annexin II is a protein that in humans is encoded by the ANXA2 gene. Annexin 2 is involved in diverse ... Kwon M, MacLeod TJ, Zhang Y, Waisman DM (Jan 2005). "S100A10, annexin A2, and annexin a2 heterotetramer as candidate ... "Entrez Gene: ANXA2 annexin A2". Kling T, Ferrarese R, Ó hAilín D, Johansson P, Heiland DH, Dai F, Vasilikos I, Weyerbrock A, ... Annexin A2 has been proposed to function inside the cell in sorting of endosomes and outside the cell in anticoagulant ...
ANXA2P3 annexin A2 pseudogene 3 [Homo sapiens] ANXA2P3 annexin A2 pseudogene 3 [Homo sapiens]. Gene ID:305 ... Role of the long non‑coding RNA‑Annexin A2 pseudogene 3/Annexin A2 signaling pathway in biliary atresia‑associated hepatic ... Title: Role of the long non‑coding RNA‑Annexin A2 pseudogene 3/Annexin A2 signaling pathway in biliary atresia‑associated ... annexin A2 pseudogene 3provided by HGNC. Primary source. HGNC:HGNC:540 Gene type. pseudo. RefSeq status. PROVISIONAL. Organism ...
Goat polyclonal Annexin A2 antibody validated for WB and tested in Human. Referenced in 2 publications and 1 independent review ... Anti-Annexin A2 antibody (ab2242) at 0.5 µg/ml + A431 lysate in RIPA buffer at 35 µg. Developed using the ECL technique.. ... Detects a band of approximately 35 kDa (predicted molecular weight: 38 kDa).Can be blocked with Human Annexin A2 peptide ( ... A pair of annexin repeats may form one binding site for calcium and phospholipid. ...
Together, these new findings suggest that manipulation of the annexin A2/S100A10 system may offer promising new avenues for ... Dysregulation of both A2 and p11 has been reported in examples of rodent and human cancer. Intracellularly, A2 plays a critical ... appreciation for the physiologic functions of annexin A2 and its associated proteins has increased dramatically. With its ... In human studies, the A2 system contributes to the coagulopathy of acute promyelocytic leukemia, and is a target of high-titer ...
Human Annexin-2/ANXA2 SimpleStep ELISA® Kit is a highly sensitive, single-wash 90 min immunoassay suitable for Cell Lysate ... The concentrations of Annexin A2 were measured in duplicate and interpolated from the Annexin A2 standard curve and corrected ... ELISA - Human Annexin A2 ELISA Kit (ab264612) Protein - Recombinant Human Annexin-2/ANXA2 protein (ab93005) SDS-PAGE, MS, WB, ... The Annexin A2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background- ...
Serum antibodies against annexin A2 were significantly elevated in subjects with proliferative LN, validating those antibodies ... annexin A2. By ELISA, patients with proliferative LN demonstrated significantly increased antibodies against annexin A2. ... Autoantibodies targeting glomerular annexin A2 identify patients with proliferative lupus nephritis Proteomics Clin Appl. 2015 ... Serum antibodies against annexin A2 were significantly elevated in subjects with proliferative LN, validating those antibodies ...
... the annexin A2 (A2) complex promotes vascular fibrinolysis. The intracellular A2 complex is a heterotetramer of two A2 monomers ... The annexin A2 system and vascular homeostasis Vascul Pharmacol. Mar-Jun 2011;54(3-6):59-67. doi: 10.1016/j.vph.2011.03.003. ... Exogenous A2 may also offer a novel therapeutic approach to ischemic thrombotic stroke, as administration of A2 in conjunction ... the annexin A2 (A2) complex promotes va … ... Over-expression of A2 is seen in acute promyelocytic leukemia ...
Rabbit Polyclonal Anti-Annexin A2 Antibody. Validated: WB, ICC/IF, IHC, IHC-P, IP. Tested Reactivity: Human, Mouse, Rat, and ... Reviews for Annexin A2 Antibody (NBP1-31310) (0) There are no reviews for Annexin A2 Antibody (NBP1-31310). By submitting a ... Diseases for Annexin A2 Antibody (NBP1-31310). Discover more about diseases related to Annexin A2 Antibody (NBP1-31310). ... PTMs for Annexin A2 Antibody (NBP1-31310). Learn more about PTMs related to Annexin A2 Antibody (NBP1-31310). ...
ANX2, ANX2L4, CAL1H, LIP2, LPC2, LPC2D, P36, PAP-IV, ANXA2, Annexin A2, Annexin-2, Annexin II, Lipocortin II, Calpactin-1 heavy ... ANXA2 is part of the annexin family and is involved in the regulation of cellular growth and in signal transduction pathways. ...
Among them, the annexin A2 (AnxA2)-S100A10 complex has been shown to participate in the tethering/docking of secretion- ... Among them, the annexin A2 (AnxA2)-S100A10 complex has been shown to participate in the tethering/docking of secretion- ... "Regulation of von-Willebrand Factor Secretion from Endothelial Cells by the Annexin A2-S100A10 Complex." Int. J. Mol. Sci. 19, ... Regulation of von-Willebrand Factor Secretion from Endothelial Cells by the Annexin A2-S100A10 Complex. Anna Holthenrich. ...
... Adam G. ... M. J. Hayes, D.-M. Shao, A. Grieve, T. Levine, M. Bailly, and S. E. Moss, "Annexin A2 at the interface between F-actin and ... D. B. N. Lee, N. Jamgotchian, S. G. Allen, F. W. K. Kan, and I. L. Hale, "Annexin A2 heterotetramer: role in tight junction ... E. M. Danielsen, B. van Deurs, and G. H. Hansen, "Nonclassical secretion of annexin A2 to the lumenal side of the enterocyte ...
We identified Annexin A2 (ANXA2), a member of the Annexin family of calcium-dependent phospholipid binding proteins, as a new ...
Annexin A2. By Technical Data. Annexin A2 - Proteins. Annexin A2 - Recombinant protein - Proteins. Human - Annexin A2 - ... Annexin A2 - for Western blotting - Proteins. Annexin A2 - for ELISA - Proteins. Annexin A2 - tag: His - Proteins. Annexin A2 ... a property not shared with monomeric annexin A2 or, as a matter of fact, any other annexin. Annexin A2 plays a role in the ... References to Annexin A2. *Deng S, Jing B, Xing T, Hou L, Yang Z. Overexpression of annexin A2 is associated with abnormal ...
Annexin A2 expression during cellular differentiation in myeloid cell lines. W.S. Gilmore, S. Olwill, H. McGlynn, H.D. ... Annexin A2 expression during cellular differentiation in myeloid cell lines. W.S. Gilmore, S. Olwill, H. McGlynn, H.D. ... Annexin A2 expression during cellular differentiation in myeloid cell lines Message Subject (Your Name) has forwarded a page to ... Annexin A2 is a calcium-dependent, phospholipid-binding protein found on many cell types. It consists of a short hydrophobic ...
OE-A2+OGD group. 3-MA, 3-methyladenine; A2, Annexin A2; OE-A2, lentiviral A2 overexpression vectors; sh-A2, lentiviral vectors ... A2, Annexin A2; sh-A2, lentiviral vectors encoding short hairpin RNAs against human A2; OE-A2, lentiviral A2 overexpression ... D) A2 luciferase activity. **P,0.01 vs. Con group; ##P,0.01 vs. OGD group. OGD, oxygen-glucose deprivation; A2, Annexin A2; HIF ... Lentiviral vectors encoding shRNAs against human A2 (sh-A2, sequence 5′-TGAGGGTGACGTTAGCATTAC-3′ for A2 shRNA), lentiviral ...
Background and Purpose-Tissue-type plasminogen activator (tPA) in combination with recombinant annexin A2 (rA2) is known to ... Effects of Tissue Plasminogen Activator and Annexin A2 Combination Therapy on Long-Term Neurological Outcomes of Rat Focal ... Effects of Tissue Plasminogen Activator and Annexin A2 Combination Therapy on Long-Term Neurological Outcomes of Rat Focal ... Effects of Tissue Plasminogen Activator and Annexin A2 Combination Therapy on Long-Term Neurological Outcomes of Rat Focal ...
EPR analysis of soluble and membrane -bound annexin A2. EPR analysis of soluble and membrane -bound annexin A2. - Page 19. Save ... Annexin A2 antibodies will abolish this plasmin generation, while the over-expression of annexin A2 in non-APL leukocytes up- ... Annexin A2 antibodies will abolish this plasmin generation, while the over-expression of annexin A2 in non-APL leukocytes up- ... Recombinant annexin A2 heterotetramer inhibits the fibrinolysis by plasmin in vitro, and annexin A2 heterotertramers has been ...
42 The N-terminus of annexin A2 contains binding sites for S100A10. In vivo, annexin A2 is often found in the form of (annexin ... 42 The N-terminus of annexin A2 contains binding sites for S100A10. In vivo, annexin A2 is often found in the form of (annexin ... EPR analysis of soluble and membrane -bound annexin A2. EPR analysis of soluble and membrane -bound annexin A2. - Page 54. Save ... In the presence of Ca2+, two annexin A2 monomers form a dimer by direct contacts at the repeat 3. These two monomers ...
This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a ... Recombinant Human Annexin A2, His-tagged. E. coli. Human. His. +Inquiry. ANXA2-1845H. Recombinant Human Annexin A2, His-tagged ... ANXA2; annexin A2; ANX2ANX2L4; CAL1H; LPC2D; LIP2; Annexin II; Annexin-2; Calpactin I heavy chain; Calpactin-1 heavy chain; ... Canas, F; Simonin, L; et al. Annexin A2 autoantibodies in thrombosis and autoimmune diseases. THROMBOSIS RESEARCH 135:226-230( ...
... with the Annexin (Anxa) proteins being the most represented family. We show that both Annexin A2 (Anxa2) transcript and protein ... Hydrogen peroxide is a neuronal alarmin that triggers specific RNAs, local translation of Annexin A2, and cytoskeletal ...
... Type. ... Annexin A2 (AnxA2) is a multi-functional and -compartmental protein whose subcellular localisation and functions are tightly ...
B and C) Annexin A2 (0.2 μg)/sialostatin L2 mobility electrophoresis/immunoblotting and ELISAs. (D) Sialostatin L2/annexin A2 ... Annexin a2−/− mice revealed enlarged spleens compared to those of control animals (Fig. 7C and D). Annexin a2−/− animals also ... bound to annexin A2 (PDB accession number 2HYW). L2, loop 2 (red); L1, loop 1 (dark blue). (B) Annexin A2 (0.2 μg) bound to ... ensued in Annexin a2−/− mice (Fig. 7F and G).. In summary, we demonstrate in this report that (i) annexin A2 regulates the ...
Annexin A2 (AnxA2) has been reported to co-precipitate with the non-structural (NS) HCV proteins NS5B and NS3/NS4A, indicating ... Annexin A2, Hepatitis C virus, Hepatocellular carcinoma, NS5B, RNA, allosteric inhibition, filibuvir, surface plasmon resonance ... Characterization of interactions between hepatitis C virus NS5B polymerase, annexin A2 and RNA - effects on NS5B catalysis and ...
... and stained for annexin A2 (red), F-actin (green), and nuclei (blue). Upper images (A-E) show only annexin A2 staining, and ... Cortical colocalization of annexin A2 and F-actin gradually increased up to 60 s. Scale bars: 10 μm and 1 μm as indicated. ... Involvement of Annexin A2 in Serum-MAF Dependent Phagocytic Activation of Macrophages. KUMPEI KAWAKATSU, MISATO MAEMURA, ... The changes in the localization of annexin A2 and F-actin. Macrophages were fixed at 0 s (A, F; Untreated), 30 s (B, G), 60 s ( ...
Annexin A2 Recombinant Protein-NP_001002857 (MBS203687) product datasheet at MyBioSource, Recombinant Proteins. Application: ... Annexin A2 UniProt Synonym Protein Names Annexin II; Annexin-2; Calpactin I heavy chain; Calpactin-1 heavy chain; Chromobindin- ... annexin A2; annexin II; annexin-2; calpactin I heavy chain; calpactin I heavy polypeptide; calpactin-1 heavy chain; ... Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated ...
We previously established subclones of GBM cells with distinct invasive phenotypes and identified annexin A2 (ANXA2) as an ... Annexin A2-STAT3-Oncostatin M receptor axis drives phenotypic and mesenchymal changes in glioblastoma. *Yuji Matsumoto1. , ... Onishi M, Ichikawa T, Kurozumi K, Inoue S, Maruo T, Otani Y, Fujii K, Ishida J, Shimazu Y, Yoshida K et al (2015) Annexin A2 ... Matsumoto, Y., Ichikawa, T., Kurozumi, K. et al. Annexin A2-STAT3-Oncostatin M receptor axis drives phenotypic and mesenchymal ...
Annexin A2 occurs as a 36-KDa monomer and in a 90-KDa complex containing two subunits of annexin A2 and two subunits of S100 ... "Annexin A2" by people in UAMS Profiles by year, and whether "Annexin A2" was a major or minor topic of these publications. ... "Annexin A2" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... Below are the most recent publications written about "Annexin A2" by people in Profiles over the past ten years. ...
anti-Annexin A2 antibody Contact Us * Alternative name. Annexin 2, annexin A2, Annexin II, ANX2, ANX2L4, ANXA2, CAL1H, ... Product Description specificalAnnexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up- ... Inquiry About anti-Annexin A2 antibody If you hope to order it or contact us directly, please contact us via [email protected] ... Immunogenannexin A2 * IHCImmunohistochemistry of paraffin-embedded human breast cancer slide using A000431( ANXA2 Antibody) at ...
Annexin A1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent ... The Annexin family of calcium-binding proteins is composed of al least thirteen mammalian genes ( ... Clone (M298) was generated from a recombinant sequence that included amino acids in the C-terminal region of rat Annexin A2. * ... Annexin A2 (calpactin I, chromobindin 8, p36, Lipocortin II, PAP-IV, or Protein I) is a cytoskeletal calcium-dependent ...
The annexin A2/S100A10 system in health and disease: Emerging paradigms. Together they form a unique fingerprint. * Annexin A2 ... The annexin A2/S100A10 system in health and disease: Emerging paradigms. Journal of Biomedicine and Biotechnology. 2012 Nov 19; ... The annexin A2/S100A10 system in health and disease: Emerging paradigms. Nadia Hedhli, Domenick J. Falcone, Bihui Huang, ... The annexin A2/S100A10 system in health and disease : Emerging paradigms. / Hedhli, Nadia; Falcone, Domenick J.; Huang, Bihui; ...
  • Annexin A2 also known as annexin II is a protein that in humans is encoded by the ANXA2 gene. (wikipedia.org)
  • Annexin 2 is involved in diverse cellular processes such as cell motility (especially that of the epithelial cells), linkage of membrane-associated protein complexes to the actin cytoskeleton, endocytosis, fibrinolysis, ion channel formation, and cell matrix interactions. (wikipedia.org)
  • Annexin II is a pleiotropic protein meaning that its function is dependent on place and time in the body. (wikipedia.org)
  • This protein is a member of the annexin family. (wikipedia.org)
  • Annexin A2 has been shown to interact with Prohibitin, CEACAM1, S100A10, PCNA, complement Factor H, and a number of viral factors including the HPV16 minor capsid protein L2. (wikipedia.org)
  • A2 is a 36-kilodalton protein produced by endothelial cells, monocytes, macrophages, trophoblast cells, and some tumor cells and exists both free in the cytoplasm and in association with intracellular and plasma membrane surfaces [ 2 , 3 ]. (hindawi.com)
  • Protein S100A10, also known as p11, is a well-described binding partner of A2 [ 7 , 8 ]. (hindawi.com)
  • Human Annexin A2 ELISA Kit (ab264612) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Annexin A2 protein in cell lysate. (abcam.com)
  • Annexin A2 is a calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. (abcam.com)
  • Bioinformatic analysis determined that 36 of those proteins were membrane associated, including a protein previously suggested to contribute to glomerulonephritis and LN, annexin A2. (nih.gov)
  • The intracellular A2 complex is a heterotetramer of two A2 monomers and two copies of the associated protein, p11. (nih.gov)
  • Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Annexin II. (novusbio.com)
  • Annexin A2 is a calcium-dependent, phospholipid-binding protein found on many cell types. (biochemsoctrans.org)
  • The results showed that OGD treatment significantly increased the mRNA and protein levels of A2 in human retinal endothelial cells (HRECs), which was dependent on activation of hypoxia inducible factor (HIF)‑1α signaling. (spandidos-publications.com)
  • As a type of Ca 2+ -regulated, phospholipid-binding protein, A2 exists both as a free monomer in the cytoplasm or tethered to the surface of the plasma membrane ( 6 ). (spandidos-publications.com)
  • We show that both Annexin A2 (Anxa2) transcript and protein accumulate at the tips of long pseudopods that Schwann cells extend upon H 2 O 2 exposure. (bionity.com)
  • Annexin A2 (AnxA2) is a multi-functional and -compartmental protein whose subcellular localisation and functions are tightly regulated by its post-translational modifications. (uib.no)
  • Recombinant Annexin A2 protein was expressed in E.coli and purified by using conventional chromatography techniques. (mybiosource.com)
  • A member of the annexin family that is a substrate for a tyrosine kinase, ONCOGENE PROTEIN PP60(V-SRC). (uams.edu)
  • Annexin A2 occurs as a 36-KDa monomer and in a 90-KDa complex containing two subunits of annexin A2 and two subunits of S100 FAMILY PROTEIN P11. (uams.edu)
  • Product Description specifical Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. (biobool.com)
  • Annexin A2 (calpactin I, chromobindin 8, p36, Lipocortin II, PAP-IV, or Protein I) is a cytoskeletal calcium-dependent phospholipid binding protein, which has been shown to be a mediator of cortocosteroid activity, a substrate for serine/threonine kinases and growth regulated tyrosine kinases, and may play a role in secretion. (ecmbio.com)
  • This antibody detects a 36 kDa* protein corresponding to the molecular mass of Annexin A2 on SDS-PAGE immunoblots of rat PC12 cells. (ecmbio.com)
  • Our results illustrated no significant change in the total Annexin A2 protein or mRNA after exposure to different doses of glucose for different times. (qscience.com)
  • Annexin A2 (ANXA2) is a pro-metastasis protein, previously identified as a relevant PDAC antigen that is expressed by a GM-CSF-secreting allogenic whole pancreatic tumor cell vaccine (GVAX) to induce an anti-ANXA2 antibody response in patients with PDAC. (biomedcentral.com)
  • Using murine macrophages deficient in the membrane phospholipid binding protein, annexin A2 (ANXA2), we observed a significant decrease in both phagocytosis of yeast cells and the frequency of nonlytic exocytosis. (elsevier.com)
  • These results imply that extracellular C-1-P, acting through the extracellular annexin a2-p11 heterotetrameric protein, can mediate vascular endothelial cell invasion. (elsevier.com)
  • Annexin A2 is a cytosolic protein abundant in EVs. (sciencematters.io)
  • Annexin A2 is a cytosolic calcium-dependent membrane-binding protein lacking a signal sequence for secretion (leaderless protein). (sciencematters.io)
  • Here we demonstrate that, despite being a cytosolic protein, Annexin A2 localises not only in the lumen but also on the surface of EVs. (sciencematters.io)
  • Yeast two-hybrid screening identified an interaction between NKCC2 and the cytosolic protein, annexin A2 (AnxA2). (mdc-berlin.de)
  • Annexin A2 (AnxA2) is a multifunctional calcium-regulated phospholipids-binding protein found in a subset of renal neoplasms. (unthsc.edu)
  • Annexin A2 (AnxA2) is an often identified exosomal protein whose elevated levels are seen in TNBC patient sera and cell lines. (unthsc.edu)
  • The peptide was derived from the protein annexin A2 and others had shown that ~10% of RA patients make autoantibodies against this self-protein. (acrabstracts.org)
  • We confirm that annexin A2, a phospholipid-binding protein that protects damaged endothelial cells, is a potential autoantigen in a subgroup of patients with RA. (acrabstracts.org)
  • Moreover, we report for the first time that this protein may serve as an autoantigen in a subgroup of patients with LA. As with reactivity to ECGF, autoantibody responses to annexin A2 in Lyme disease seem to occur as a part of the immune response to the infection, whereas additional factors, such as immune dysregulation, are required for refractory arthritis. (acrabstracts.org)
  • We aimed to determine whether annexin A2 (ANXA2), a Ca2 +-dependent and phospholipid-binding protein, participates in cell junction dynamics. (popcouncil.org)
  • Annexin A2 (ANXA2), a phospholipid-binding protein, has multiple biological functions depending on its cellular localization. (elsevier.com)
  • Annexin A2 , a multifunctional tumor associated protein , promotes nuclear factor-kappa B (NF-κB) activation by interacting with NF-κB p50 subunit and facilitating its nuclear translocation. (bvsalud.org)
  • It harbours binding sites for cytoplasmic protein ligands that can be targeted to membranes through the annexin-core-mediated phospholipid interaction. (nature.com)
  • Our data indicate that annexin A2 protein level is upregulated, whereas annexin Al and a actinin 4 expression are downregulated in tumours with lymph node metastasis compared to those without lymphatic spread. (edu.au)
  • Annexin A2 and insulin-like growth factor-binding protein 2 were overexpressed in cancer but not in chronic pancreatitis, making them promising biomarker candidates for pancreatic cancer. (mcponline.org)
  • from annexins and phospholipase A2 activating protein (PLAP). (bio.net)
  • Since its discovery as a src kinase substrate more than three decades ago, appreciation for the physiologic functions of annexin A2 and its associated proteins has increased dramatically. (hindawi.com)
  • While the core domains of the annexin proteins are relatively well conserved, the hydrophilic amino-terminal "tail" or "interaction" domains are highly variable and essentially unique to each family member. (hindawi.com)
  • The Annexins are a family of structurally similar proteins. (novusbio.com)
  • 1990). Further details concerning the S100 proteins and their interactions with annexins will be discussed later in this paper. (usc.edu)
  • Among the genes positively regulated at both levels, we identified an enriched cluster involved in cytoskeleton remodeling and cell migration, with the Annexin (Anxa) proteins being the most represented family. (bionity.com)
  • Annexin A2 (AnxA2) has been reported to co-precipitate with the non-structural (NS) HCV proteins NS5B and NS3/NS4A, indicating a role in HCC tumorigenesis and effect on DAA therapy. (diva-portal.org)
  • Annexin A2 belongs to a family of calcium-dependent membrane and phospholipid binding proteins. (mybiosource.com)
  • Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. (mybiosource.com)
  • The Annexin family of calcium-binding proteins is composed of al least thirteen mammalian genes (Annexin A1-13). (ecmbio.com)
  • Annexins mediate lipid raft-dependent trafficking of transmembrane proteins, including the AVP-regulated water channel, aquaporin 2. (mdc-berlin.de)
  • We have previously identified prohibitin (PHB) and annexin A2 (ANX2) as proteins interacting on the surface of vascular endothelial cells in white adipose tissue (WAT) of humans and mice. (onetownmanyvoices.com)
  • Annexin A2 (ANXA2) is a member of the annexin family of calcium-dependent proteins expressed in many cells [ 9 ], which share structural and functional features [ 10 ]. (biomedcentral.com)
  • Annexins are a multigene family of Ca 2+ -regulated proteins that are characterized by a unique Ca 2+ - and membrane-binding module - the annexin core domain. (nature.com)
  • A unique class of these proteins - annexins - can bind to certain membrane phospholipids in a Ca 2+ -dependent manner, providing a link between Ca 2+ signalling and membrane functions. (nature.com)
  • By forming networks on the membrane surface, annexins can function as organizers of membrane domains and membrane-recruitment platforms for proteins with which they interact. (nature.com)
  • Liemann, S. & Lewit-Bentley, A. Annexins: a novel family of calcium- and membrane-binding proteins in search of a function. (nature.com)
  • The annexins are a family of Ca2+-dependent phospholipidbinding proteins with various membrane-related functions. (paperity.org)
  • Combined the experimental data in the urine and in tumor tissues collected from patients with UTUC, the crucial over-expressed proteins are calreticulin, annexin A2, and annexin A3. (beds.ac.uk)
  • Human Annexin A13 (ANXA13), also known as Annexin XIII or Annexin 13, is a 35 kDa member of the Annexin family (1), which are calcium-dependent phospholipid-binding proteins being preferentially located on the cytosolic face of the plasma membrane. (novusbio.com)
  • Annexin A1 and A2 proteins were also relatively abundant in the cells. (usda.gov)
  • These include several immunoglobulin chains, chemokine and ribosomal proteins, annexin A2, vav 1 and several S100 signaling proteins. (medsci.org)
  • Detects a band of approximately 35 kDa (predicted molecular weight: 38 kDa).Can be blocked with Human Annexin A2 peptide (ab22880) . (abcam.com)
  • Quantitate Human Annexin A2 with 1.86 ng/ml sensitivity. (abcam.com)
  • Immunohistochemistry-Paraffin: Annexin A2 Antibody [NBP1-31310] - Analysis of paraffin-embedded Human pancreas tissue, using ANXA2 antibody at 1:100 dilution. (novusbio.com)
  • Western Blot: Annexin A2 Antibody [NBP1-31310] - A. 30 ug A549 whole cell lysate/extract, B. 30 ug HCT116 whole cell lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:1000. (novusbio.com)
  • Western Blot: Annexin A2 Antibody [NBP1-31310] - Analysis. (novusbio.com)
  • J. H. Rand and X. X. Wu, "Antibody-mediated disruption of the annexin-V antithrombotic shield: a new mechanism for thrombosis in the antiphospholipid syndrome," Thrombosis Research , vol. 114, no. 5-6, pp. 383-389, 2004. (hindawi.com)
  • Inquiry About anti-Annexin A2 antibody If you hope to order it or contact us directly, please contact us via [email protected] (biobool.com)
  • The antibody has been shown to label Annexin A2 in immunocytochemical analysis of PC12 cells and immunohistochemical analysis of mouse diaphragm. (ecmbio.com)
  • The blot was probed with mouse monoclonal anti-Annexin A2 antibody at 1:250 (lane 1) or 1:1000 (lane 2). (ecmbio.com)
  • We tested serum samples from our cohort of RA patients for anti-annexin A2 autoantibodies, and for comparison, from healthy control subjects or from patients with antibiotic-responsive or antibiotic-refractory LA. In our RA cohort, sera from 24% of 91 patients had antibody responses to annexin A2 that were >3SD above the mean value in healthy control subjects (Figure). (acrabstracts.org)
  • In annexin A2-positive RA patients, the magnitude of antibody responses to ACPA or RF were less than in annexin A2-negative patients. (acrabstracts.org)
  • Recombinant annexin A2 heterotetramer inhibits the fibrinolysis by plasmin in vitro, and annexin A2 heterotertramers has been identified as a plasminogen reductase that facilitates the release of an anti-angiogenic plasmin fragment called A61(Choi, Ghuman et al. (usc.edu)
  • 2002). These results reveal the intriguing role of annexin A2 heterotetramer in the processes of fibrinolysis, blood clotting and angiogenesis(Hayes and Moss 2004). (usc.edu)
  • In vivo, annexin A2 is often found in the form of (annexin A2)2 (S100A10)2 heterotetramer (A2t) , which usually exists in the extracellular matrix (ECM). (usc.edu)
  • S100A10 is also known as p11 or calpactin I light chain, mostly existing in vivo in the annexin A2 heterotetramer. (usc.edu)
  • We have now identified and characterized interactions between ceramide 1-phosphate and the annexin a2-p11 heterotetramer constituents. (elsevier.com)
  • Annexins bind to phospholipids and may be involved in regulation of membrane transport, membrane channel activity, and interaction of the cell membrane with the extracellular matrix. (novusbio.com)
  • Annexin A2 is located on the surface of extracellular vesicles. (sciencematters.io)
  • To investigate the packaging and localisation of Annexin A2 in extracellular vesicles. (sciencematters.io)
  • Extracellular annexin functions that have been substantiated by mouse knockout models are anti-inflammatory and fibrinolytic activities. (nature.com)
  • Figure 5: Annexins have specialized extracellular roles. (nature.com)
  • By ELISA, patients with proliferative LN demonstrated significantly increased antibodies against annexin A2. (nih.gov)
  • Proteomic approaches identified multiple candidate antigens for autoantibodies in patients with LN. Serum antibodies against annexin A2 were significantly elevated in subjects with proliferative LN, validating those antibodies as potential biomarkers. (nih.gov)
  • Over-expression of A2 is seen in acute promyelocytic leukemia during the early hemorrhagic phase, while high titer antibodies to A2, as in antiphospholipid syndrome or cerebral venous thrombosis, are associated with thrombosis. (nih.gov)
  • Annexin A2 antibodies will abolish this plasmin generation, while the over-expression of annexin A2 in non-APL leukocytes up-regulates that occurrence (Menell, Cesarman et al. (usc.edu)
  • With its binding partner S100A10 (p11), A2 forms a cell surface complex that regulates generation of the primary fibrinolytic protease, plasmin, and is dynamically regulated in settings of hemostasis and thrombosis. (hindawi.com)
  • Together, these new findings suggest that manipulation of the annexin A2/S100A10 system may offer promising new avenues for treatment of a spectrum of human disorders. (hindawi.com)
  • Among them, the annexin A2 (AnxA2)-S100A10 complex has been shown to participate in the tethering/docking of secretion-competent WPB at the plasma membrane, and interference with AnxA2/S100A10 expression or complex formation significantly reduces acute WPB exocytosis and VWF release. (mdpi.com)
  • Annexin A2 forms heterotetrameric complex containing the S100A10 dimer and two annexin A2 chains significantly alters the properties of this annexin in vitro and also within cells. (biovendor.com)
  • Importantly, the annexin A2-S100A10 complex can aggregate membrane vesicles at micromolar Ca2+ levels, a property not shared with monomeric annexin A2 or, as a matter of fact, any other annexin. (biovendor.com)
  • 42 The N-terminus of annexin A2 contains binding sites for S100A10. (usc.edu)
  • A peptide-binding affinity assay suggests that the first 14 amino acids of the annexin A2 N-terminus are involved in the binding of S100A10, with the first 12 regarded as crucial (Becker, Weber et al. (usc.edu)
  • Heterotetramerization of S100A10 (p11) with Annexin A2 and translocation of this complex to the cell surface is required to modulate the activation of plasmin. (qscience.com)
  • The role of annexin II tetramer in the activation of plasminogen," The Journal of Biological Chemistry , vol. 273, no. 8, pp. 4790-4799, 1998. (hindawi.com)
  • In the present study, we have used flow cytometry and real-time PCR to investigate the role of annexin A2 expression in the proliferation and differentiation of a number of myeloid cell lines. (biochemsoctrans.org)
  • Background and Purpose- Tissue-type plasminogen activator (tPA) in combination with recombinant annexin A2 (rA2) is known to reduce acute brain damage after focal ischemia. (ahajournals.org)
  • Clone (M298) was generated from a recombinant sequence that included amino acids in the C-terminal region of rat Annexin A2. (ecmbio.com)
  • Intracellularly, A2 plays a critical role in endosomal repair in postarthroplastic osteolysis, and intracellular p11 regulates serotonin receptor activity in psychiatric mood disorders. (hindawi.com)
  • Annexin II regulates fibrin homeostasis and neoangiogenesis in vivo," The Journal of Clinical Investigation , vol. 113, no. 1, pp. 38-48, 2004. (hindawi.com)
  • However, the underlying mechanism by which A2 regulates retinal neovascularization remains unclear. (spandidos-publications.com)
  • Taken together, the results of the present study suggested that promoting retinal endothelial cell survival by autophagy activation via the HIF‑1α signaling pathway in a hypoxic and ischemic microenvironment may underlie the mechanism by which A2 regulates retinal neovascularization. (spandidos-publications.com)
  • We hypothesize that the Annexin A2-p11 system regulates the development of retinal angiogenesis and may be a potential therapeutic target in diabetic retinopathy. (qscience.com)
  • It was reported that mice deficient in the A2 gene were resistant to hypoxia-related retinal neovascularization in a model of diabetic retinopathy ( 3 ). (spandidos-publications.com)
  • The human A2 gene is located on chromosome 15 (15q21) and is expressed in multiple human cells, including endothelial cells, monocytes, macrophages, dendritic cells, trophoblast cells, and tumor cells ( 6 , 7 ). (spandidos-publications.com)
  • This gene encodes a member of the annexin family. (creativebiomart.net)
  • This study was purposed to investigate the apoptosis-inducing effect of Annexin A2 gene (AnxA2) on multiple myeloma (MM) cells and its mechanisms. (bvsalud.org)
  • Ferret Annexin A2/ANXA2 Gene ORF cDNA clone expression plasmid, C-GFPSpark tag (FG60086-ACG) - available for purchase from ABclonal - allows for easy subcloning of the ORF (pCMV3-C-GFPSpark Vector) to other plasmid vectors. (abclonal.com)
  • This raises fundamental questions regarding Annexin A2 transport to the outer leaflet of the EV membrane as it lacks a signal peptide for secretion. (sciencematters.io)
  • Epigenetic regulation of Annexin A2 has been identified as a key determinant of mesenchymal transformation in brain tumors. (wikipedia.org)
  • ANXA2 is part of the annexin family and is involved in the regulation of cellular growth and in signal transduction pathways. (prospecbio.com)
  • Annexin A2 plays a role in the regulation of cellular growth and in signal transduction pathways. (biovendor.com)
  • In the present study, oxygen‑glucose deprivation (OGD) was used to mimic the hypoxic and ischemic microenvironment, to observe the role of A2 in retinal neovascularization regulation by focusing on autophagy. (spandidos-publications.com)
  • Overall our data illustrates a role for high glucose in post-translational regulation of Annexin A2 and p11. (qscience.com)
  • Wang, CY & Lin, CF 2014, ' Annexin A2: Its molecular regulation and cellular expression in cancer development ', Disease Markers , vol. 2014, 308976. (elsevier.com)
  • Figure 3: The regulation of membrane-actin interactions by annexin A2. (nature.com)
  • Members of the Annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways (3, 4). (novusbio.com)
  • Furthermore, knockdown of A2 decreased cell viability and promoted cell apoptosis under OGD conditions. (spandidos-publications.com)
  • Overexpression of A2 increased cell viability and reduced cell apoptosis under OGD conditions, and inhibiting autophagy using an inhibitor, reversed these changes, suggesting that upregulation of A2 by OGD serves a cytoprotective role by inducing cell autophagy in HRECs. (spandidos-publications.com)
  • The authors' previous study additionally demonstrated that A2 silencing inhibited the proliferation and enhanced the apoptosis of HUVECs ( 11 ). (spandidos-publications.com)
  • Over expression of K302A Annexin A2 , a mutant version of Annexin A2 , which fails to interact with G-Rg5 and G-Rk1, effectively reduced the NF-κB inhibitory effect and apoptosis induced by G-Rg5 and G-Rk1. (bvsalud.org)
  • In addition, the knockdown of Annexin A2 largely enhanced NF-κB activation and apoptosis induced by the two molecules, indicating that the effects of G-Rg5 and G-Rk1 on NF-κB were mainly mediated by Annexin A2 . (bvsalud.org)
  • Western blotting analysis and immunohistochemistry of tumor tissues and normal tissues from patients with UTUC were carried out to further verify five possible UTUC biomarkers, including zinc-alpha-2-glycoprotein, calreticulin, annexin A2, annexin A3 and haptoglobin. (beds.ac.uk)
  • As a co-receptor for plasminogen and tissue plasminogen activator (tPA), which are key factors in plasmin generation, the annexin A2 (A2) complex promotes vascular fibrinolysis. (nih.gov)
  • This group had previously reported that homocysteine impairs endothelial cell surface plasminogen activation by posttranslationally modifying annexin A2, the coreceptor for plasminogen and tissue plasminogen activator. (jci.org)
  • The Annexin family has been implicated as regulators of such diverse processes as ion-flux, endocytosis and exocytosis, and cellular adhesion. (ecmbio.com)
  • abstract = "We have established a pair of animal models (J3T-1 and J3T-2) with different invasive and angiogenic phenotypes, and demonstrated that annexin A2 is expressed at higher levels in J3T-1 than J3T-2 cells. (elsevier.com)
  • abstract = "Annexin A2 (ANXA2) orchestrates multiple biologic processes and clinical associations, especially in cancer progression. (elsevier.com)
  • Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion. (mybiosource.com)
  • Deng S, Jing B, Xing T, Hou L, Yang Z. Overexpression of annexin A2 is associated with abnormal ubiquitination in breast cancer. (biovendor.com)
  • Annexin A6 reverses transformation of A431 cells after overexpression, and this effect may involve annexin A6 targeting of p120 RasGAP to the plasma membrane to inactivate Ras. (ecmbio.com)
  • Stable silencing or overexpression of annexin A2 in J3T-1 and J3T-2 cells (J3T-1shA and J3T-2A cells) was established and used. (elsevier.com)
  • Positive expression of annexin A2 was observed in tumor cells surrounding dilated vessels in 25/30 human glioblastoma specimens. (elsevier.com)
  • Taken together, this study for the first time demonstrated that G-Rg5 and G-Rk1 inhibit tumor cell growth by targeting Annexin A2 and NF-κB pathway, and G-Rg5 and G-Rk1 might be promising natural compounds for targeted cancer therapy . (bvsalud.org)
  • Additionally, A2 −/− mice also displayed reduced angiogenesis in three different models of postnatal angiogenesis ( 5 ). (spandidos-publications.com)
  • Knockdown of the A2 receptor (A2R) significantly inhibited the proliferation, adhesion, migration and tube formation of human umbilical vein endothelial cells (HUVECs), which is usually used in the in vitro study of angiogenesis ( 10 ). (spandidos-publications.com)
  • We previously established subclones of GBM cells with distinct invasive phenotypes and identified annexin A2 (ANXA2) as an activator of angiogenesis and perivascular invasion. (biomedcentral.com)
  • The function of annexin A2 in relation to angiogenesis and invasion was investigated using these models. (elsevier.com)
  • We identify annexin A2 as a factor regulating angiogenesis and invasion of malignant gliomas. (elsevier.com)
  • They now show that an annexin A2-deficient mouse rendered hyperhomocysteinemic by dietary means has impaired fibrinolysis, perivascular fibrin persistence, and attenuated angiogenesis (angiostasis). (jci.org)
  • In epithelial cells, it may account for up to 95% of total annexin A2 (Harder and Gerke 1993). (usc.edu)
  • A pair of annexin repeats may form one binding site for calcium and phospholipid. (abcam.com)
  • When A2 is membrane associated, the tightly packed, alpha-helical 33-kDa core domain forms a disk whose convex face is associated with membrane phospholipid and whose concave face is oriented away from the membrane. (hindawi.com)
  • The Annexins consist of a unique N-terminal domain followed by a homologous C-terminal core domain containing the phospholipid-binding sites. (novusbio.com)
  • In response to endothelial cell activation, A2 is phosphorylated by src-kinase, and translocated to the cell surface in a highly regulated manner. (nih.gov)
  • Annexin A2 is reported to be a powerful activator of plasminogen and, therefore, is implicated in many normal and pathological processes such as haemostasis and metastasis. (biochemsoctrans.org)
  • Annexin A5 has also been associated with the blood coagulation process and has been shown to promote urokinase-type plasminogen activators. (usc.edu)
  • Interfering with intracellular annexin function, by overexpressing mutants or by using RNA-interference-mediated downregulation, has different effects depending on the annexin being targeted. (nature.com)
  • T. Harder, R. Kellner, R. G. Parton, and J. Gruenberg, "Specific release of membrane-bound annexin II and cortical cytoskeletal elements by sequestration of membrane cholesterol," Molecular Biology of the Cell , vol. 8, no. 3, pp. 533-545, 1997. (hindawi.com)
  • White arrowheads indicate cortical annexin A2 staining. (iiarjournals.org)
  • Large and small arrowheads indicate the cortical localization of F-actin and annexin A2, respectively. (iiarjournals.org)
  • Cortical colocalization of annexin A2 and F-actin gradually increased up to 60 s. (iiarjournals.org)
  • Annexins possess a series of unique 70 amino acid annexin-repeat modules that form a curved disk and allow for the creation of an intercessory complex between plasma membrane and cytofilaments. (biovendor.com)
  • Annexin A2 may, therefore, be an important player in cellular differentiation and its disorders. (biochemsoctrans.org)
  • Here we demonstrated that two ginsenosides Rg5 (G-Rg5) and Rk1 (G-Rk1), with similar structure, directly bound to Annexin A2 by molecular docking and cellular thermal shift assay. (bvsalud.org)
  • Annexin A2 has been implicated in a number of biochemical processes, including cell proliferation, foetal immune tolerance, ion-channel activation, cell-cell interactions and the bridging of membranes. (biochemsoctrans.org)
  • This core domain enables Ca 2+ -bound annexins to peripherally dock onto membranes that contain negatively charged phospholipids. (nature.com)
  • Figure 2: Annexin assemblies on membranes. (nature.com)
  • The name annexin is derived from the Greek annex, which means bring/hold together, and was chosen to describe the principal property of all, or at least nearly all, annexins the binding to and possibly holding together of certain biological structures, in particular membranes [1]. (paperity.org)
  • Leukocytes from those APL patients have a high level of annexin A2 on the surface, and stimulate plasmin generation twice as efficiently as normal leukemic cells. (usc.edu)
  • Blank and filled arrowheads indicate areas of localisation of only F-actin or annexin A2 or areas where they are colocalized, respectively. (iiarjournals.org)
  • El-Gezawy E, Abdelbaki L, Deen M.Z, Eldeek S and Abdelgawad M.I. Expression of circulating annexin A2 in hepatic diseases and hepatocellular carcinoma . (hoajonline.com)
  • It has been reported that Annexin A2 (ANXA2) is up-regulated in hepatocellular carcinoma (HCC), but the roles of ANXA2 in the migration and invasion of HCC cells have not been determined. (paperity.org)
  • In the present study, we found that annexin A2 (ANXA2) is upregulated expressed in porcine alveolar macrophages infected with PRRSV. (biomedcentral.com)
  • Phosphorylation of Annexin A2. (uib.no)
  • The monomeric form of annexin A2 was formerly referred to as calpactin I heavy chain. (uams.edu)
  • and ( i , j , k ) Annexin A2-heparin complex. (nih.gov)
  • Here, we discuss the role of the A2 system in vascular homeostasis, the molecular interactions that regulate its profibrinolytic activity, and its potential role in the pathogenesis and treatment of vascular disease. (nih.gov)
  • 2017. "Annexin A2 is critical for blood-testis barrier integrity and spermatid disengagement in the mammalian testis," Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 1864(3): 527-545. (popcouncil.org)
  • Swairjo, M. A. & Seaton, B. A. Annexin structure and membrane interactions: a molecular perspective. (nature.com)
  • Characterization of interactions between hepatitis C virus NS5B polymerase, annexin A2 and RNA - effects on NS5B catalysis and allosteric inhibition. (diva-portal.org)
  • STVHEILCKLSLEGD , corresponding to N terminal amino acids 2-16 of Human Annexin II. (abcam.com)
  • Among mammalian species for which A2 has been sequenced, identity is approximately 98% at the amino acid level. (hindawi.com)
  • Contains 4 annexin repeats. (abcam.com)
  • The C-terminal domain of Annexin A13 is comprised of four 60‑70 aa annexin repeats which form a tightly packed disc known as the Annexin domain. (novusbio.com)
  • In human studies, the A2 system contributes to the coagulopathy of acute promyelocytic leukemia, and is a target of high-titer autoantibodies in patients with antiphospholipid syndrome, cerebral thrombosis, and possibly preeclampsia. (hindawi.com)
  • After Anxa3 downregulation, the growth of A549 and LTEP-a2 LADC cells was slowed and they showed decreased migration and invasion in vitro. (cancerindex.org)
  • There are at least ten different annexins in mammalian species. (mybiosource.com)
  • Analysis of CD44-containing lipid rafts: recruitment of annexin II and stabilization by the actin cytoskeleton," The Journal of Cell Biology , vol. 146, no. 4, pp. 843-854, 1999. (hindawi.com)
  • Annexin A2 expression in head and neck squamous cell carcinoma]. (biovendor.com)
  • The results demonstrated that annexin A2 expression was affected when the cells were induced to differentiate by stimulation with all- trans -retinoic acid. (biochemsoctrans.org)
  • Annexin A2 (ANXA2) plays an important role in the pathogenesis of multiple malignancies particularly HCCs and its expression strongly also affects the outcomes of HCC patients. (hoajonline.com)
  • Objective of current study is to determine the effect of hyperglycemia on Annexin A2 and p11 expression in endothelial cells. (qscience.com)
  • To address this objective we examined effect of high glucose on Annexin A2 and p11 expression in HUVEC cells. (qscience.com)
  • Biotinylation studies illustrated an increased cell surface expression of both Annexin A2 and p11 under high-dose glucose conditions. (qscience.com)
  • Thirty human glioblastoma samples were evaluated for expression of annexin A2, vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF). (elsevier.com)
  • Immunohistochemical and quantitative reverse-transcription polymerase chain reaction analyses revealed higher expression of annexin A2, VEGF and PDGF in J3T-1 and J3T-2A cells. (elsevier.com)
  • Here, we show a co-regulated upregulation in the expression of Annexin A2 (AnxA2), a known substrate of Src and one of the regulators of EGFR receptor endocytosis, in Herceptin resistant and Her-2 negative breast cancer. (tdl.org)
  • Several groups, including ours, have reported that annexin A2 (ANXA2) expression is reduced in most prostate cancer (CaP). (elsevier.com)
  • Among these, the expression levels of annexin A2 and tubulin β chain, were most strongly upregulated in the fibroblasts of patients with GBA‑PD and PARK2‑PD, respectively. (spandidos-publications.com)
  • The expression of the uroplakin and annexin genes was detected in the cells, although only UPK1B, UPK3B, ANXA2 and ANXA4 were detected in fetal pig pancreatic duct tissue. (usda.gov)
  • Sarkar S, Maxwell CA, Luthra G, Singal A, Qiu S, Bauer VP, Okorodudu AO, Singh P. Annexina2 is Increasingly Expressed and Released Into the Serum of Patients Positive for Colonic Growths/Tumors in Relation to Disease Progression: Diagnostic Implications. (biovendor.com)
  • The changes in the localization of annexin A2 and F-actin just after addition of serum-derived macrophage activating factor (MAF). (iiarjournals.org)
  • Both Rg5 and Rk1 inhibited the interaction between Annexin A2 and NF-κB p50 subunit, their translocation to nuclear and NF-κB activation. (bvsalud.org)
  • Each annexin contains a second, highly varible region - the N-terminal interaction domain. (nature.com)