A member of the annexin family that is a substrate for a tyrosine kinase, ONCOGENE PROTEIN PP60(V-SRC). Annexin A2 occurs as a 36-KDa monomer and in a 90-KDa complex containing two subunits of annexin A2 and two subunits of S100 FAMILY PROTEIN P11. The monomeric form of annexin A2 was formerly referred to as calpactin I heavy chain.
Protein of the annexin family exhibiting lipid interaction and steroid-inducibility.
A protein of the annexin family isolated from human PLACENTA and other tissues. It inhibits cytosolic PHOSPHOLIPASE A2, and displays anticoagulant activity.
Protein of the annexin family with a probable role in exocytotic and endocytotic membrane events.
Protein of the annexin family originally isolated from the electric organ of the electric ray Torpedo marmorata. It has been found in a wide range of mammalian tissue where it is localized to the apical membrane of polarized EPITHELIAL CELLS.
An annexin family member that plays a role in MEMBRANE FUSION and signaling via VOLTAGE-DEPENDENT CALCIUM CHANNELS.
A protein of the annexin family that catalyzes the conversion of 1-D-inositol 1,2-cyclic phosphate and water to 1-D-myo-inositol 1-phosphate.
Family of calcium- and phospholipid-binding proteins which are structurally related and exhibit immunological cross-reactivity. Each member contains four homologous 70-kDa repeats. The annexins are differentially distributed in vertebrate tissues (and lower eukaryotes) and appear to be involved in MEMBRANE FUSION and SIGNAL TRANSDUCTION.
A family of highly acidic calcium-binding proteins found in large concentration in the brain and believed to be glial in origin. They are also found in other organs in the body. They have in common the EF-hand motif (EF HAND MOTIFS) found on a number of calcium binding proteins. The name of this family derives from the property of being soluble in a 100% saturated ammonium sulfate solution.
Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a serine moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and serine and 2 moles of fatty acids.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Quaternary ammonium analog of ethidium; an intercalating dye with a specific affinity to certain forms of DNA and, used as diiodide, to separate them in density gradients; also forms fluorescent complexes with cholinesterase which it inhibits.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.
Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
A cell line derived from cultured tumor cells.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Extracellular vesicles generated by the shedding of CELL MEMBRANE blebs.
A family of G-protein-coupled receptors that was originally identified by its ability to bind N-formyl peptides such as N-FORMYLMETHIONINE LEUCYL-PHENYLALANINE. Since N-formyl peptides are found in MITOCHONDRIA and BACTERIA, this class of receptors is believed to play a role in mediating cellular responses to cellular damage and bacterial invasion. However, non-formylated peptide ligands have also been found for this receptor class.
Proteins prepared by recombinant DNA technology.
A short pro-domain caspase that plays an effector role in APOPTOSIS. It is activated by INITIATOR CASPASES such as CASPASE 9. Isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Organic compounds that contain technetium as an integral part of the molecule. These compounds are often used as radionuclide imaging agents.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Established cell cultures that have the potential to propagate indefinitely.
A family of intracellular CYSTEINE ENDOPEPTIDASES that play a role in regulating INFLAMMATION and APOPTOSIS. They specifically cleave peptides at a CYSTEINE amino acid that follows an ASPARTIC ACID residue. Caspases are activated by proteolytic cleavage of a precursor form to yield large and small subunits that form the enzyme. Since the cleavage site within precursors matches the specificity of caspases, sequential activation of precursors by activated caspases can occur.
Fluorescent probe capable of being conjugated to tissue and proteins. It is used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.
An in situ method for detecting areas of DNA which are nicked during APOPTOSIS. Terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 prime OH ends of either single- or double-stranded DNA. The terminal deoxynucleotidyl transferase nick end labeling, or TUNEL, assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA FRAGMENTATION.
A product of the lysis of plasminogen (profibrinolysin) by PLASMINOGEN activators. It is composed of two polypeptide chains, light (B) and heavy (A), with a molecular weight of 75,000. It is the major proteolytic enzyme involved in blood clot retraction or the lysis of fibrin and quickly inactivated by antiplasmins.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
Conditions in which the production of adrenal CORTICOSTEROIDS falls below the requirement of the body. Adrenal insufficiency can be caused by defects in the ADRENAL GLANDS, the PITUITARY GLAND, or the HYPOTHALAMUS.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Serum containing GAMMA-GLOBULINS which are antibodies for lymphocyte ANTIGENS. It is used both as a test for HISTOCOMPATIBILITY and therapeutically in TRANSPLANTATION.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
The pathological process occurring in cells that are dying from irreparable injuries. It is caused by the progressive, uncontrolled action of degradative ENZYMES, leading to MITOCHONDRIAL SWELLING, nuclear flocculation, and cell lysis. It is distinct it from APOPTOSIS, which is a normal, regulated cellular process.
The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES).
Cell surface proteins that bind LIPOXINS with high affinity and trigger intracellular changes influencing the behavior of cells.
A chemically diverse group of substances produced by various tissues in the body that cause slow contraction of smooth muscle; they have other intense but varied pharmacologic activities.
Trihydroxy derivatives of eicosanoic acids. They are primarily derived from arachidonic acid, however eicosapentaenoic acid derivatives also exist. Many of them are naturally occurring mediators of immune regulation.
White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES).
Pathological processes of CORONARY ARTERIES that may derive from a congenital abnormality, atherosclerotic, or non-atherosclerotic cause.
Substances that reduce or suppress INFLAMMATION.
The veins and arteries of the HEART.
Radiography of the vascular system of the heart muscle after injection of a contrast medium.
An imbalance between myocardial functional requirements and the capacity of the CORONARY VESSELS to supply sufficient blood flow. It is a form of MYOCARDIAL ISCHEMIA (insufficient blood supply to the heart muscle) caused by a decreased capacity of the coronary vessels.

Down-regulation of microglial cyclo-oxygenase-2 and inducible nitric oxide synthase expression by lipocortin 1. (1/385)

1. Activated microglial cells are believed to play an active role in most brain pathologies, during which they can contribute to host defence and repair but also to the establishment of tissue damage. These actions are largely mediated by microglial secretory products, among which are prostaglandins (PGs) and nitric oxide (NO). 2. The anti-inflammatory protein, lipocortin 1 (LC1) was reported to have neuroprotective action and to be induced by glucocorticoids in several brain structures, with a preferential expression in microglia. In this paper we tested whether the neuroprotective effect of LC1 could be explained by an inhibitory effect on microglial activation. 3. We have previously shown that bacterial endotoxin (LPS) strongly stimulates PGE2 and NO production in rat primary microglial cultures, by inducing the expression of the key enzymes cyclo-oxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), respectively. 4. Dexamethasone (DEX, 1-100 nM) and LC1-derived N-terminus peptide (peptide Ac2-26, 1-100 microg ml(-1)) dose-dependently inhibited the production of both PGE2 and NO from LPS-stimulated microglia. The inhibitory effects of DEX on NO and of the peptide on NO and PGE2 synthesis were partially abrogated by a specific antiserum, raised against the N-terminus of human LC1. The peptide Ac2-26 did not affect arachidonic acid release from control and LPS-stimulated microglial cultures. 5. Western blot experiments showed that the LPS-induced expression of COX-2 and iNOS was effectively down-regulated by DEX (100 nM) and peptide Ac2-26 (100 microg ml(-1)). 6. In conclusion, our findings support the hypothesis that LC1 may foster neuroprotection by limiting microglial activation, through autocrine and paracrine mechanisms.  (+info)

Modulation of cellular annexin I in human leukocytes infiltrating DTH skin reactions. (2/385)

Based on our previous studies showing endogenous annexin I being depleted from migrated neutrophils (PMN) in vitro, we have tested whether the levels of this glucocorticoid-regulated protein in PMN and mononuclear cells (PBMC) were modified after adhesion to endothelial monolayers in vitro and extravasation into skin blisters in vivo. In vitro, annexin I levels were depleted more significantly (-70%) in post-adherent PMNs than in monocytes (-25%) and lymphocytes (-50%, only in the positive fraction). In vivo, a significant time-dependent increase (approximately threefold, P < 0.05) in cell-associated annexin I was measured in PBMCs recovered from the blisters, whereas no significant changes were detected in extravasated PMNs. This was associated with annexin I release in the blister fluids (approximately 35 ng/mL), whereas no detectable protein was found in matched-paired plasmas. In conclusion, we report for the first time an activation of the annexin I pathway during an ongoing experimental inflammatory response in humans, which is differently regulated between PMNs and PBMCs.  (+info)

Inhibitory effect of annexin I on synovial inflammation in rat adjuvant arthritis. (3/385)

OBJECTIVE: Annexin I is an endogenous antiinflammatory mediator, expressed in rheumatoid arthritis (RA) synovium, the contribution of which to autoregulation of the synovial inflammatory response has not been examined in models of RA. We investigated the antiinflammatory role of annexin I in rat adjuvant arthritis. METHODS: Rats with adjuvant-induced arthritis (AIA) were treated with a specific anti-annexin I monoclonal antibody (mAb), isotype control IgG, and/or dexamethasone. Clinical outcomes and synovial synthesis of tumor necrosis factor alpha (TNFalpha), prostaglandin E2 (PGE2), and nitric oxide were examined, and annexin I expression was assessed by flow cytometry and reverse transcription-polymerase chain reaction. RESULTS: Anti-annexin I mAb reversed the effects of dexamethasone on the clinical features of AIA and exacerbated AIA in the absence of exogenous glucocorticoid. Clinical exacerbation of AIA by anti-annexin I mAb was accompanied by significantly increased synovial TNFalpha and PGE2, suggesting that annexin I tonically inhibits the production of these mediators. Anti-annexin I mAb treatment was associated with significantly reduced leukocyte intracellular annexin I, despite increased annexin I messenger RNA expression, consistent with a depletion effect of extracellular mAb via the cell surface. CONCLUSION: Annexin I is a key endogenous inhibitory mediator of arthritis via mechanisms that include inhibition of cytokine and effector molecule production. Moreover, a synthesis-independent depletion of intracellular annexin I by extracellular antibody supports the hypothesis that externalization of annexin I is involved in its mode of action.  (+info)

Annexin I modulates cell functions by controlling intracellular calcium release. (4/385)

Annexin I is an intracellular protein in search of a function. Ex vivo it has calcium- and phospholipid-binding properties. To evaluate its role in vivo, MCF-7 cells were stably transfected with annexin I in sense or antisense orientations. In cells overexpressing annexin I, calcium release was abrogated on stimulation of purinergic or bradykinin receptors, whereas non-transfected cells or cells with down-regulated annexin I released calcium within seconds. Basal calcium and calcium stores were not affected. The impaired calcium release was paralleled by a down-regulation of the activities of phospholipase C, group II phospholipase A2, and E-cadherin with altered adhesion and enhanced tumor growth on soft agar. Significantly smaller tumors, with the histologically most differentiated cells, were observed in nude mice inoculated with cells transfected with the antisense rather than with the sense plasmid. These observations indicate that annexin I modulates cell functions by controlling intracellular calcium release. Frey, B. M., Reber, B. F. X., Vishwanath, B. S., Escher, G., Frey, F. J. Annexin I modulates cell functions by controlling intracellular calcium release.  (+info)

The inhibitory effect of dexamethasone on lymphocyte adhesion molecule expression and intercellular aggregation is not mediated by lipocortin 1. (5/385)

Glucocorticoids exert their anti-inflammatory activity through multiple pathways which include the inhibition of cell adhesion events. The glucocorticoid-induced protein lipocortin 1 (LC1) has reported anti-inflammatory properties and has been proposed as a putative mediator of the anti-inflammatory effects of glucocorticoids. The role of LC1 in mediating the glucocorticoid inhibition of lymphocyte adhesion and cell adhesion molecule (CAM) expression was investigated in vitro using a microaggregation assay, flow cytometry and confocal microscopy. Lymphocytes stimulated for 96 h with plastic-bound OKT3 antibody showed significant increases in LFA-1 and CD2 expression. Dexamethasone (DEX; 10(-6) M) inhibited this increase but the neutralizing anti-LC1 MoAb 1A (5 microg/ml) failed to reverse the DEX effect; neither was purified human LC1 (50 x 10(-9) M) able to inhibit CAM expression. The biological activity of the LC1 was confirmed by its ability to suppress monocyte phagocytosis and respiratory burst in response to bovine serum albumin (BSA)-anti-BSA complexes. OKT3 stimulation of cultured mononuclear cells resulted in intercellular aggregation, scored microscopically using a visual index. This aggregation was completely reversed by 10-6 M DEX but unaffected by LC1 (50 x 10(-9) M). Significant intracellular expression of lymphocyte LC1 was observed using the anti-LC1 MoAb 1B in saponin-permeabilized cells. Distribution of LC1 had a diffuse, cytoplasmic pattern. LC1 expression was reduced following 3 h treatment with 10(-6) M DEX. These findings indicate that the DEX effects on lymphocyte adhesion and CAM expression are not mediated by LC1. Thus the reported in vivo effects of LC1 on leucocyte adhesion and transmigration probably occur through functional/conformation changes of surface CAM, rather than by alteration in expression.  (+info)

The annexin protein lipocortin 1 regulates the MAPK/ERK pathway. (6/385)

Lipocortin 1 (annexin 1) is a calcium- and phospholipid-binding protein that modulates anti-inflammatory responses including those induced by lipopolysaccharide. To investigate the precise role of lipocortin 1 in regulating the lipopolysaccharide-induced signal transduction pathways, we generated stable RAW 264.7 macrophage cell lines expressing decreased and increased lipocortin 1 protein. Several RAW 264.7 clones with increased lipocortin 1 protein levels showed constitutive activation of the mitogen-activated protein kinase extracellular signal-regulated kinase, which was down-regulated following lipopolysaccharide treatment. Conversely, clones with decreased lipocortin 1 protein expression showed prolonged extracellular signal-regulated kinase activity, following lipopolysaccharide activation. Lipocortin 1 specifically regulates the components of the extracellular signal-regulated kinase pathway, since changes in lipocortin 1 protein expression had no affect on the related mitogen-activated protein kinases p38 and c-Jun N-terminal kinase. Lipocortin 1 modulated upstream components of the extracellular signal-regulated kinase pathway and associated with the adaptor protein growth factor binding protein. The downstream consequences of altered extracellular signal-regulated kinase activity were independent of the proinflammatory transcription factor nuclear factor kappa B. These data indicate that lipocortin 1 specifically regulates proximal signaling components of the extracellular signal-regulated kinase signal transduction pathway, resulting in the modulation of biochemical functions in RAW macrophages.  (+info)

Structural basis of the Ca(2+)-dependent association between S100C (S100A11) and its target, the N-terminal part of annexin I. (7/385)

BACKGROUND: S100C (S100A11) is a member of the S100 calcium-binding protein family, the function of which is not yet entirely clear, but may include cytoskeleton assembly and dynamics. S100 proteins consist of two EF-hand calcium-binding motifs, connected by a flexible loop. Like several other members of the family, S100C forms a homodimer. A number of S100 proteins form complexes with annexins, another family of calcium-binding proteins that also bind to phospholipids. Structural studies have been undertaken to understand the basis of these interactions. RESULTS: We have solved the crystal structure of a complex of calcium-loaded S100C with a synthetic peptide that corresponds to the first 14 residues of the annexin I N terminus at 2.3 A resolution. We find a stoichiometry of one peptide per S100C monomer, the entire complex structure consisting of two peptides per S100C dimer. Each peptide, however, interacts with both monomers of the S100C dimer. The two S100C molecules of the dimer are linked by a disulphide bridge. The structure is surprisingly close to that of the p11-annexin II N-terminal peptide complex solved previously. We have performed competition experiments to try to understand the specificity of the S100-annexin interaction. CONCLUSIONS: By solving the structure of a second annexin N terminus-S100 protein complex, we confirmed a novel mode of interaction of S100 proteins with their target peptides; there is a one-to-one stoichiometry, where the dimeric structure of the S100 protein is, nevertheless, essential for complex formation. Our structure can provide a model for a Ca(2+)-regulated annexin I-S100C heterotetramer, possibly involved in crosslinking membrane surfaces or organising membranes during certain fusion events.  (+info)

Detection of annexins I and IV in bronchoalveolar lavage fluids from calves inoculated with bovine herpes virus-1. (8/385)

Annexins are phospholipid-binding proteins and are abundant in the lung. Annexins I and IV, but not II and VI, have been detected in bronchoalveolar lavage (BAL) fluids from calves inoculated with Pasteurella haemolytica, the pathogen for calf pneumonia. In this study, BAL fluids from calves with experimental pneumonia induced by inoculation to right lung lobes of bovine herpes virus-1 (BHV-1), the major viral pathogen for pneumonia, were examined for detection of annexins I and IV. Of 6 calves inoculated with BHV-1, annexins I and IV were coincidentally detected in BAL fluids from right lung lobes of 4 calves, but not in BAL fluids from left lung lobes of 6 inoculated calves or those from left and right lung lobes of 3 control calves. Annexin II and VI were not found in any BAL fluids examined. These results, together with previous findings on calves inoculated with Pasteurella haemolytica, suggest that the release of annexins I and IV onto the alveolar surface is an essential event occurring in response to pulmonary infections of BHIV-1 and Pasteurella haemolytica.  (+info)

Fingerprint Dive into the research topics of Negative regulatory role of Annexin-A1 in 14-3-3η-mediated glucocorticoid receptor transcriptional activation. Together they form a unique fingerprint. ...
Annexin 1 is an anti-inflammatory protein that plays a key role in innate immunity by modulating the activation of several types of cells, including neutrophils. Here we have developed a cleavage assay using tagged annexin 1 and observed marked activity in the membrane fraction of activated neutrophils. A combination of inhibitors, transfected cells, and proteomic analyses allowed us to identify proteinase 3 as the main enzyme responsible for this cleavage in the N terminus region of the protein, at least in the context of neutrophil activation. Because annexin 1 is an important endogenous anti-inflammatory mediator, blocking its cleavage by proteinase 3 would augment its homeostatic pro-resolving actions and could represent an opportunity for innovative anti-inflammatory drug discovery.. ...
Professor Morands is a physician-scientist whose key interests are clinical outcome measurement and biological profiling in SLE, and the actions of glucocorticoid induced proteins on the immune system. Read more at https://www.monash.edu/medicine/scs/research/cid/rheumatology/researchers/eric-morand His group has pioneered the development of treat-to-target outcome measures in SLE, via the AsiaPacific Lupus Collaboration, which has over 1800 patients under study. Biomarker studies in SLE are also a major interest, with 000s of samples linked to longitudinal clinical data archived in the Australian Lupus Registry & Biobank, headquartered at Monash. His lab discovered the role in rheumatic disease of multiple important glucocorticoid-regulated proteins. Most recently his group defined the role of GILZ in RA, Th17 pathways, B cell activation, and lupus, and are working on exploiting its actions to develop a safe glucocorticoid mimic. His lab also works on innate immunity including the actions of ...
Bird diseases can have a vast impact on humans, so understanding their immune systems can be a benefit for people. An important element in the immune system of
Introduction: Adipose tissue oxygen consumption and blood flow are altered in obesity inducing local tissue hypoxia. Hypoxia affects several biological functions including adipogenesis, inflammation, insulin resistance and metabolism affecting the overall degree of adiposity. The expression of Annexin A1 (ANXA1); an anti-inflammatory protein, increases in the adipose tissue in response to obesity and further increases in the subcutaneous adipose tissue of old obese individuals compared to young obese individuals however, the roles of ANXA1 in the adipose tissue or obesity are unclear.. Aim: The aim of this study was to investigate the potential roles of ANXA1 in adipocytes using an in vitro model of obesity.. Methods: Differentiated SGBS cells were treated with and without 10 μM AC2-26 peptide and incubated in hypoxia (1% O2) for 24 hours to achieve obesogenic conditions. Gene expressions were analysed via RT-qPCR and normalised against GAPDH. Statistical analysis was preformed using GraphPad ...
Rationale:. The investigators hypothesize that EPO protects against apoptosis after acute ischemia in man and that it is detectable using the annexin-A5 model.. Objective:. Does infusion of a single dose of Epoetin Alfa, a short-acting EPO, protect against apoptosis in man after acute ischemia?. Study design:. A double blinded randomised cross-over study.. Study population:. 12 Healthy male volunteers, between 18 and 40 years old.. Intervention:. All 12 volunteers will receive a single dose of EPO and placebo in a randomized order. A six week wash-out period is obtained in order to avoid interference of both treatments.. Main study parameters/endpoints:. The percentage of difference between radioactivity (quantified as counts per pixel) of the experimental and control thenar muscle at one and four hours after reperfusion. ...
A small, external bioreactor holding human cells pumped out an anti-inflammatory protein to prevent organ damage and other complications in a rat with acute inflammation caused by bacterial products in a model of sepsis, ...
Rabbit polyclonal Annexin-2/ANXA2 (phospho S26) antibody. Validated in WB and tested in Human. Immunogen corresponding to synthetic peptide.
Complete information for ANXA1 gene (Protein Coding), Annexin A1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Complete information for ANXA1 gene (Protein Coding), Annexin A1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
New research from University of London has revealed that the female sex hormone estrogen may be able to keep the bodys immune systems in check, thus helping to protect women from cardiovascular disease.. The study states that estrogen works on white blood cells so that they do not stick to the insides of blood vessels - a process known to cause dangerous blockages.. The findings of this study might successfully explain why men seem to be more affected by cardiovascular disease than women, and why women are at a higher risk post-menopause.. The research consisted of a comparison between blood samples from pre-menopausal women and men. The blood cells from pre-menopausal women were found to have high levels of annexin-A1 on the surface of the white blood cells. The estrogen moves the annexin-A1 from storage within the white blood cells to the surface. This is what prevents sticking of the cells to the walls of blood vessels, which causes vascular damage.. Image: Shutterstock. ...
Annexin A2 is a calcium-dependent, phospholipid-binding protein found on many cell types. It consists of a short hydrophobic tail (Ser2-Asn32), which dictates its function, and a core domain (Phe33-Asp339), which is involved in phospholipid binding. Annexin A2 has been implicated in a number of biochemical processes, including cell proliferation, foetal immune tolerance, ion-channel activation, cell-cell interactions and the bridging of membranes. Annexin A2 is reported to be a powerful activator of plasminogen and, therefore, is implicated in many normal and pathological processes such as haemostasis and metastasis. Myeloid cell lines are used, extensively, to study many aspects of cellular proliferation, differentiation and function. In the present study, we have used flow cytometry and real-time PCR to investigate the role of annexin A2 expression in the proliferation and differentiation of a number of myeloid cell lines. The results demonstrated that annexin A2 expression was affected when ...
In our hands, the trypsin actually reduced the Annexin staining presumably by stripping off all the phosphotidyl-serine on the surface along with the surface proteins (adhesion molecules). The only time we have seen enhanced annexin is when the cells suffer enough trauma in harvesting that the membrane is not intact and the Annexin gets inside the cell and attaches to the ps on the inner membrane. Julie At 02:11 PM 8/18/98, you wrote: , ,I have a client who is staining with Annexin-FITC. They are looking at ,cell lines and are using trypsin to get the cells off the flasks. We are ,seeing what we think is an exagerated Annexin response. Does anyone out ,there have any feedback on how trypsin affects Annexin staining? Any ,experience and or information about this will be greatly appreciated. , Thanks in advance, , Joan Kalnitsky ,Joan Kalnitsky ,Flow Cytometry Laboratory ,VMRCVM ,(540) 231-4115 ,FAX 540-231-7367 , , It is better to serve than to receive , B. Borg , , , , , ...
Gentaur molecular products has all kinds of products like :search , Ray Biotech \ Recombinant Human Annexin IX \ 228-10059-2 for more molecular products just contact us
Gentaur molecular products has all kinds of products like :search , QED \ Human Annexin A9 \ 13823P-1000 for more molecular products just contact us
The Annexin family of calcium-binding proteins is composed of al least thirteen mammalian genes (Annexin A1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. The Annexin family
Non-structural protein 1 (NS1) is a multifunctional protein and a crucial regulatory factor in the replication and pathogenesis of avian influenza virus (AIV). Studies have shown that NS1 can interact with a variety of host proteins to modulate the viral life cycle. We previously generated a monoclonal antibody against NS1 protein; In the current research study, using this antibody, we immunoprecipitated host proteins that interact with NS1 to better understand the roles played by NS1 in communications between virus and host. Co-immunoprecipitation experiments identified annexin A2 (ANXA2) as a target molecule interacting with NS1. Results from confocal laser scanning microscopy indicated that NS1 co-localized with ANXA2 in the cell cytoplasm. Overexpression of ANXA2 significantly increased the titer of H5N1 subtype HPAIV, whereas siRNA-mediated knockdown of ANXA2 markedly inhibited the expression of viral proteins and reduced the progeny virus titer. Our results indicate that ANXA2 interacts with NS1
Proteolytic cleavage of haemagglutinin (HA) is essential for the infectivity of influenza A viruses (IAVs). This is usually mediated by trypsin-like proteases present in the respiratory tract. However, the ability to use plasminogen (PLG) as an alternative protease may contribute to pathogenesis of IAV infections and virus replication outside the respiratory tract. It was demonstrated previously that neuraminidase (NA) of the IAV strain A/WSN/33 can sequester PLG, allowing this virus to replicate in a PLG-dependent fashion. However, PLG also promotes replication of other IAVs, although its mode of action is poorly understood. Here, using NA-deficient viruses, we demonstrate that NA is not required for the binding of PLG and subsequent cleavage of HA. However, we demonstrate that the cellular protein annexin 2 (A2) can bind PLG and contributes to PLG-dependent cleavage of HA and subsequent IAV replication. Collectively, these results indicate that PLG promotes IAV replication in an A2-dependent fashion
We have shown that PPAR-α agonists exert rapid and profound antinociceptive effects in animal models of acute, persistent inflammatory, and neuropathic pain, which are contingent on PPAR-α expression. These effects are comparable in time course and magnitude to those elicited by the clinically used analgesic gabapentin and are not associated with the development of tolerance. Thus, our results reveal a previously unsuspected role of PPAR-α in pain modulation and suggest that this nuclear receptor may provide a novel target for broad-spectrum analgesic drugs.. PPAR-α regulates systemic inflammation by inducing the expression of anti-inflammatory proteins, such as IκB-α, repressing the expression of proinflammatory proteins, such as tumor necrosis factor-α, and limiting the recruitment of immune cells to inflammation sites (Kostadinova et al., 2005). This array of effects is mediated through two separate but converging mechanisms-the induction of responsive target genes and the inhibition ...
This graph shows the total number of publications written about Annexin A3 by people in this website by year, and whether Annexin A3 was a major or minor topic of these publications ...
Annexin A11 Antibody 10479-2-AP has been identified with IF, IHC, IP, WB, ELISA. 10479-2-AP detected 56-60 kDa band in HeLa cells with 1:500-1:2000 dilution...
购买我们的重组人Annexin A3蛋白。Ab92929为全长蛋白,在大肠杆菌中生产并经过Western blot, SDS-PAGE实验验证。Abcam提供免费的实验方案,操作技巧及专业的支持。
TY - JOUR. T1 - The membrane phospholipid binding protein annexin A2 promotes phagocytosis and nonlytic exocytosis of cryptococcus neoformans and impacts survival in fungal infection. AU - Stukes, Sabriya. AU - Coelho, Carolina. AU - Rivera, Johanna. AU - Jedlicka, Anne E.. AU - Hajjar, Katherine A.. AU - Casadevall, Arturo. PY - 2016/8/15. Y1 - 2016/8/15. N2 - Cryptococcus neoformans is a fungal pathogen with a unique intracellular pathogenic strategy that includes nonlytic exocytosis, a phenomenon whereby fungal cells are expunged from macrophages without lysing the host cell. The exact mechanism and specific proteins involved in this process have yet to be completely defined. Using murine macrophages deficient in the membrane phospholipid binding protein, annexin A2 (ANXA2), we observed a significant decrease in both phagocytosis of yeast cells and the frequency of nonlytic exocytosis. Cryptococcal cells isolated from Anxa2-deficient (Anxa2-/-) bone marrow-derived macrophages and lung ...
The inflammatory process has been described as a crucial mechanism in the pathophysiology of temporal lobe epilepsy. The anti-inflammatory protein annexin A1 (ANXA1) represents an interesting target in the regulation of neuroinflammation through the inhibition of leukocyte transmigration and the release of proinflammatory mediators. In this study, the role of the ANXA1-derived peptide Ac2-26 in an experimental model of status epilepticus (SE) was evaluated. Male Wistar rats were divided into Naive, Sham, SE and SE+Ac2-26 groups, and SE was induced by intrahippocampal injection of pilocarpine. In Sham animals, saline was applied into the hippocampus, and Naive rats were only handled. Three doses of Ac2-26 (1 mg/kg) were administered intraperitoneally (i.p.) after 2, 8 and 14 h of SE induction. Finally, 24 h after the experiment-onset, rats were euthanized for analyses of neuronal lesion and inflammation. Pilocarpine induced generalised SE in all animals, causing neuronal damage, and systemic treatment
Using biochemical, epifluorescence and electron microscopic techniques in a U937 model system, we investigated the effect of anti-allergic drugs di-sodium cromoglycate and sodium nedocromil on the trafficking and release of the anti-inflammatory protein Annexin-A1 (Anx-A1) when this was triggered by glucocorticoid (GC) treatment. GCs alone produced a rapid (within 5min) concentration-dependent activation of PKCalpha/beta (Protein Kinase C; EC 2.7.11.13) and phosphorylation of Anx-A1 on Ser(27). Both phosphoproteins accumulated at the plasma membrane and Anx-A1 was subsequently externalised thereby inhibiting thromboxane (Tx) B(2) generation. When administered alone, cromoglycate or nedocromil had little effect on this pathway however, in the presence of a fixed sub-maximal concentration of GCs, increasing amounts of the cromoglycate-like drugs caused a striking concentration-dependent enhancement of Anx-A1 and PKCalpha/beta phosphorylation, membrane recruitment and Anx-A1 release from cells ...
The role of the calcium- and phospholipid-binding protein annexin I (ANXA1) in cell cycle regulation has been investigated in estrogen receptor (ER)-positive MCF-7 and ER-negative MDA-MB-231 breast tumor cell lines. In MCF-7 cells, ANXA1-targeting small interfering RNA (siRNA) reduced ANXA1 mRNA and protein levels and attenuated cell proliferation induced by FCS, estradiol, or epidermal growth factor. Well-characterized agonists for the known ANXA1 receptor, FPR2, including the ANXA1 N-terminal proteolytic product ANXA1(2-26), lipoxin A(4) (LXA(4)), and the synthetic peptide, Trp-Lys-Tyr-Met-Val-D-Met (WKYMVm), stimulated proliferation of MCF-7 and MDA-MB-231 cells that was attenuated by incubation with FPR2 antagonists WRW(4) (1 μM) or Boc2 (100 nM) or by siRNA against FPR2. FCS-induced mitogenic responses were attenuated by each of the FPR antagonists and by siRNA against FPR2 and, to a lesser extent, FPR1. LXA(4) increased phosphorylation of Akt, p70(S6K) but not ERK1/2. Increases in cyclin ...
Mouse anti Human Annexin A3 antibody, clone 4F1 recognizes human annexin A3, also known as Annexin III, Inositol 1,2-cyclic phosphate 2-ph
Annexin A1 is an intracellular calcium/phospholipid-binding protein that is involved in membrane organization and the regulation of the immune system. It has been attributed an anti-inflammatory role at various control levels, and recently we could show that annexin A1 externalization during secondary necrosis provides an important fail-safe mechanism counteracting inflammatory responses when the timely clearance of apoptotic cells has failed. As such, annexin A1 promotes the engulfment of dying cells and dampens the postphagocytic production of proinflammatory cytokines. In our current follow-up study, we report that exposure of annexin A1 during secondary necrosis coincided with proteolytic processing within its unique N-terminal domain by ADAM10. Most importantly, we demonstrate that the released peptide and culture supernatants of secondary necrotic, annexin A1-externalizing cells induced chemoattraction of monocytes, which was clearly reduced in annexin A1- or ADAM10-knockdown cells. Thus, ...
Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. The alternative splicing event is tissue specific and the mRNA containing the cassette exon is prevalent in brain, heart and skeletal muscle. The transcripts also differ in their 3-non coding regions by the use of two alternative poly(A) signals. Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, ...
Early in the apoptosis process, phosphatidylserine (PS) becomes exposed on the cell surface by flipping from the inner to outer leaflet of the cytoplasmic membrane. This event is thought to be important for macrophage recognition of cells undergoing apoptosis, thus allowing the cells to be rapidly phagocytosed. The binding of Annexin V to phosphatidylserine is calcium-dependent, reversible, but very tight, with a Kd of approximately 5 x 10-10 M. At low PS concentration, a binding ratio of 8 annexins to one PS has been reported. These features make Annexin V conjugates ideal for identifying membrane changes associated with apoptosis, by using either flow cytometric or in situ labeling methods. TACS® Annexin V kits are supplied with an Annexin V conjugate, an optimized binding buffer and propidium iodide. Cells are harvested and washed, then incubated with an Annexin V conjugate in binding buffer, for 15 minutes at room temperature prior to direct analysis. Alternatively, a secondary streptavidin ...
Purpose: : To examine the role of annexin 2 in the formation and internalisation of phagosomes in retinal pigment epithelial (RPE) cells. Methods: : Human ARPE19 cells were cultured on the glass inserts of Matek dishes for immunofluorescence and live imaging, or plastic Nunc dishes for biochemical analysis. In some studies ARPE19 cells were exposed to siRNA for annexin 2 for three days prior to experimentation. Outer segments were prepared from porcine retina using standard protocols (typically 50 - 100 eyes per prep), and either used fresh in biochemical experiments, or labelled with an appropriate fluorescent marker prior to imaging experiments. Results: : Depletion of annexin 2 using siRNA led to a significant quantitative reduction in the phagocytosis of rod outer segments in cultured RPE cells. In cells expressing annexin 2, imaged by confocal microscopy, a striking enrichment of annexin 2 and F-actin was observed at the phagocytic cup immediately upon engagement of the apical cell surface ...
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis. Alternatively spliced transcript variants have been described. [provided by RefSeq, Aug 2010 ...
The annexin superfamily is comprised of a range of Ca2+ dependent phospholipid binding proteins. Each of these proteins are metamorphic, which means that they possesses the capacity to dynamically and reversibly refold themselves into two or more stable conformations. Annexin A5 (ANXA5) has been linked with a variety of cellular functions including: endocytosis, Ca2+ transfer, anticoagulation, membrane repair and exocytosis. Although this protein has been characterised as having pH-dependent and Ca2+-dependent phosphatidylserine (PS) binding the full extent of these mechanisms are not understood.. In this study the changes in ANXA5 conformation as it transitions through its binding states was investigated. Recombinant human ANXA5 was produced in E. coli and subjected to analysis by circular dichroism (CD) and Förster resonance energy transfer (FRET). This revealed that the Ca2+ driven metamorphic refolding of ANXA5 involves minor adjustments of the protein to facilitate trimerisation upon PS ...
The ubiquitin E3 protein ligase Nedd4-2 is a physiological regulator of the epithelial sodium channel ENaC, which is essential for transepithelial Na+ transport and is linked to Liddles syndrome, an autosomal dominant disorder of human salt-sensitive hypertension. Nedd4-2 function is negatively regulated by phosphorylation via a serum- and glucocorticoid-inducible protein kinase (Sgk1), which serves as a mechanism to inhibit the ubiquitination-dependent degradation of ENaC. We report here that 14-3-3 proteins participate in this regulatory process through a direct interaction with a phosphorylated form of human Nedd4-2 (a human gene product of KIAA0439, termed hNedd4-2). The interaction is dependent on Sgk1-catalyzed phosphorylation of hNedd4-2 at Ser-468. We found that this interaction preserved the activity of the Sgk1-stimulated ENaC-dependent Na+ current while disrupting the interaction decreased ENaC density on the Xenopus laevis oocytes surface possibly by enhancing Nedd4-2-mediated ...
|p|Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry
Analysis of lymphocytes in patients with Plasmodium vivax malaria and its relation to the annexin-A1 and IL-10: BackgroundMalaria is the most prevalent parasiti
Invitrogen™ eBioscience™ Annexin V, Recombinant Protein Unlabeled; 300μg Invitrogen™ eBioscience™ Annexin V, Recombinant Protein...
In the present study, we show that both CRP and annexin A5 specifically bind to oxidized LDL. Via coupling to Fcγ receptors on macrophages and independent of complement, CRP subsequently enhances the association of oxidized LDL to macrophages. Enhanced association of oxidized LDL to macrophages via CRP-Fcγ receptor interaction may lead to enhanced macrophage uptake of oxidized LDL. Moreover, we show that annexin A5 binds to a site at oxidized LDL different from the CRP binding site and that annexin A5 does not antagonize the CRP effect.. Previously, Chang et al also observed specific binding of CRP to oxidized LDL,7 characterized by inhibition by whole molecules as well as F(ab′)2 fragments of EO6, an IgM antibody that binds specifically to the phosphorylcholine head group of oxidized phospholipids but not to the same moiety on nonoxidized phospholipids.26 In the present study, for characterization of binding sites, we used the monoclonal antibody DLH3 (also IgM), which recognizes an epitope ...
Annexin A6 (AnxA6) has been implicated in the regulation of endo-/exocytic pathways, cholesterol transport, and the formation of multifactorial signaling complexes in many different cell types. More recently, AnxA6 has also been linked to triglyceride storage in adipocytes. Here we investigated the potential role of AnxA6 in fatty acid (FA) induced lipid droplet (LD) formation in hepatocytes. AnxA6 was associated with LD from rat liver and HuH7 hepatocytes. In oleic acid (OA) -loaded HuH7 cells, substantial amounts of AnxA6 bound to LD in a Ca2+-independent manner. Remarkably, stable or transient AnxA6 overexpression in HuH7 cells led to elevated LD numbers/size and neutral lipid staining under control conditions as well as after OA loading compared to controls. In contrast, overexpression of AnxAl, AnxA2 and AnxA8 did not impact on OA-induced lipid accumulation. On the other hand, incubation of AnxA6-depleted HuH7 cells or primary hepatocytes from AnxA6 KO-mice with OA led to reduced FA ...
Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 has 45 to 59% identity with other members of its family and shares a similar size and exon-intron organization. Isolated from human placenta, ANX4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells ...
Annexin A2 (AnxA2) is a multi-functional and -compartmental protein whose subcellular localisation and functions are tightly regulated by its post-translational modifications. AnxA2 and its Tyr23-phosphorylated form (pTyr23AnxA2) are involved in malignant cell transformation, metastasis and angiogenesis. Here, we show that H2O2 exerts rapid, simultaneous and opposite effects on the Tyr23 phosphorylation status of AnxA2 in two distinct compartments of rat pheochromocytoma (PC12) cells. Reactive oxygen species induce dephosphorylation of pTyr23AnxA2 located in the PML bodies of the nucleus, whereas AnxA2 associated with F-actin at the cell cortex is Tyr23 phosphorylated. The H2O2-induced responses in both compartments are transient and the pTyr23AnxA2 accumulating at the cell cortex is subsequently incorporated into vesicles and then released to the extracellular space. Blocking nuclear export by leptomycin B does not affect the nuclear pool of pTyr23AnxA2, but increases the amount of total AnxA2 ...
Annexin II antibody (annexin A2) for ICC/IF, IHC-P, IP, RipA, WB. Anti-Annexin II pAb (GTX32446) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
The atherosclerotic process is considered to be driven by an imbalance between proand anti-inflammatory actions. Still, the inflammatory state in patients with coronary artery disease (CAD) remains to be clarified. Annexin A1 (AnxA1) is a glucocorticoidinduced protein which may have a key role in the anti-inflammatory response as a mediator of glucocorticoid effects.. The general aim of this thesis was to deepen the knowledge of pro- and antiinflammatory mechanisms in CAD via phenotypic assessments of immune cell subsets, in particular CD56+ T cells, and exploration of AnxA1. The long-term goal is to reveal basic mechanisms that will lead to the development of biomarkers, which may be used for individualized treatment and monitoring.. The AnxA1 protein was constitutively expressed in both neutrophils and peripheral blood mononuclear cells (PBMCs). However, it varied considerably across PBMC subsets, being most abundantly expressed in monocytes. The AnxA1 expression was also higher in CD56+ T ...
Annexin A1兔多克隆抗体(ab65844)可与小鼠, 大鼠, 人样本反应并经WB, IP, IHC, ICC实验严格验证。所有产品均提供质保服务,中国75%以上现货。
Annexin V is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Annexin V is a monomer, binds ATRX and EIF5B, and interacts with hepatitis B virus (HBV ...
ANXA2, a member of the annexin family, is a calcium-dependent phospholipid-binding protein that plays a role in the regulation of cellular growth and in signal transduction pathways. Its affinity for calcium is greatly enhanced by anionic phospholipids, and it binds two calcium ions with high affinity. ANXA2 may be involved in heat-stress response. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. It may also cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis.
Order monoclonal and polyclonal Annexin A13 antibodies for many applications. Selected quality suppliers for anti-Annexin A13 antibodies.
Buy ANXA2 recombinant protein, Annexin A2 Recombinant Protein-NP_001002857 (MBS203687) product datasheet at MyBioSource, Recombinant Proteins. Application: SDS-PAGE
SCAN HISTORY OWL11_0 2 100 NSINGLE OWL17_1 2 110 NSINGLE OWL18_0 1 115 NSINGLE OWL19_1 1 125 NSINGLE OWL21_1 1 125 NSINGLE OWL26_0 1 125 NSINGLE SPTR37_9f 2 237 NSINGLE INITIAL MOTIF SETS ANNEXIN1 Length of motif = 23 Motif number = 1 Annexin motif I - 1 PCODE ST INT KTKGVDEVTIVNILTNRSNAQRQ LUHU36 46 46 KTKGVDEVTIINILTNRSNEQRQ ANX2_CHICK 46 46 TVKGVDEATIIDILTKRNNAQRQ ANX1_CAVCU 56 56 MVKGVDEATIIDILTKRNNAQRQ LUHU 55 55 KGIGTDEATIIDIVTHRSNAQRQ ANX6_MOUSE 376 376 KGMGTDEETILKILTSRNNAQRQ ANX5_CHICK 29 29 KGIGTNEQAIIDVLTKRSNTQRQ ANX8_HUMAN 35 35 KGFGTDEQEIIDVLVGRSNQQRQ DROANNX 29 29 RGIGTDEKMLISILTERSNAQRQ ANX3_HUMAN 32 32 KGLGTDEDAIINVLAYRSTAQRQ ANX4_BOVIN 27 27 KGFGTDEQAIVDVVANRSNDQRQ HUMSNEXIN 177 177 TAKGVDEATIIDIMTTRTNAQRP ANX1_COLLI 51 51 ANNEXIN2 Length of motif = 17 Motif number = 2 Annexin motif II - 1 PCODE ST INT LKSALSGHLETVILGLL LUHU36 86 17 LKSALSGHLEAVILGLL ANX2_CHICK 86 17 LKKALTGHLEEVVLALL ANX1_CAVCU 96 17 LKKALTGHLEEVVLALL LUHU 95 17 LKSEISGDLARLILGLM ANX6_MOUSE 416 17 ...
Abcam provides specific protocols for Anti-Annexin A10 antibody (ab2343) : Western blot protocols, Immunohistochemistry protocols
1HVD: Structural and electrophysiological analysis of annexin V mutants. Mutagenesis of human annexin V, an in vitro voltage-gated calcium channel, provides information about the structural features of the ion pathway, the voltage sensor and the ion selectivity filter
BioAssay record AID 502910 submitted by ChEMBL: Induction of annexin 2-mediated cytoskeleton aggregate accumulation in human HepG2 cells at 4 uM after 2 hrs by confocal laser scanning microscopy.
Bachem offers H-3196 Annexin A1 (1-25) (dephosphorylated) (human) for your research. Find all specific details here. Find product specific information including available pack sizes, CAS, detailed description and references here.
Mouse Monoclonal Anti-Annexin A3 Antibody (2F3). Validated: WB, ICC/IF, IHC, IHC-P. Tested Reactivity: Human, Monkey. 100% Guaranteed.
Purified Recombinant Mouse Anxa1 Protein, His-tagged from Creative Biomart. Recombinant Mouse Anxa1 Protein, His-tagged can be used for research.
Annexin A2 is a calcium regulated protein involved in membrane binding and actin polymerization. In this paper, Kevin Moreau and colleagues identified Annexin A2 as a…. ...
It has been reported that Annexin A2 (ANXA2) is up-regulated in hepatocellular carcinoma (HCC), but the roles of ANXA2 in the migration and invasion of HCC ...
Expression of ANXA6 (ANX6) in cancer tissue. The cancer tissue page shows antibody staining of the protein in 20 different cancers.
Human ANXA2 full-length ORF ( AAH09564.1, 1 a.a. - 339 a.a.) recombinant protein with GST-tag at N-terminal. (H00000302-P01) - Products - Abnova
Main / Clearance & Specials / Effet zoloft 50 Le traitement par la sertraline doit être débuté à la dose de 50 mg/jour. Trouble panique, ESPT et Trouble Anxiété Sociale. Le traitement sera débuté à la dose de 25 mg/jour. Après une semaine, la dose sera augmentée à 50 mg une fois par jour. Ce schéma posologique a montré quil réduisait la fréquence des effets Prix de vente TTC‎: ‎1,95 €. Si vous arrêtez de prendre ZOLOFT 50 mg, gélule»). Une augmentation du risque de fractures a été observée chez les patients prenant ce type de médicaments. Déclaration des effets secondaires. Si vous ressentez un quelconque effet indésirable, parlez-en à votre médecin ou votre ‎Description médicament · ‎Informations · ‎Utilisation.. Mit Hurghada Reisen unternehmen Sie einen sehr intressanten Tagesausflug von Serotonin Bay nach Tirol in kleinen Gruppen und deutschsprachige Reisen effet zoloft 50 unvergesslichen Ausflug zu den berühmten Pyramiden von Gizeh sowie der ...
Fmoc-D-Lys(Boc)-OHNα-9-Fluorenylmethoxycarbonyl-Nε-t-butyloxycarbonyl-D-lysine | Fmoc-D-Lys(Boc)2411 1 g | 95.00 EUR 2411 5 g ...
... interleukin 5 and annexin A1). DRESS syndrome: Key elements promoting tissue injury in the DRESS syndrome are: Th2 cells and ...
Annexin A1 List of cutaneous conditions "Hairy cell leukemia: MedlinePlus Medical Encyclopedia". medlineplus.gov. Retrieved ...
The protein Annexin A1 (ANXA1), found in high quantities in the anterior pituitary gland, is located specifically in the ...
... annexin A1 (an inhibitor of formation of pro-inflammatory metabolites of polyunsaturated fatty acids), and the gaseous ...
"Activation of the annexin A1 pathway underlies the protective effects exerted by estrogen in polymorphonuclear leukocytes". ...
... a genetic disorder Annexin A1, a human protein Outer membrane phospholipase A1, a bacterial protein receptors α-1-Adrenoceptor ... member A1 Replication protein A1 S100 calcium binding protein A1 Sec61 alpha 1 Serum amyloid A1 Solute carrier family 35 (CMP- ... member A1 UDP glucuronosyltransferase 1 family, polypeptide A1 Urea Transporter A1 a gene found in the maize encoding for the ... member A1 Aldo-keto reductase family 1, member A1 Alpha-1-microglobulin/bikunin precursor Apolipoprotein A1 and ApoA-1 Milano ...
Annexin *A1. *A2. *A5. *Fibulin *FBLN1. *FBLN2. *FBLN5. *Vitamin D-dependent calcium-binding protein/Calbindin ...
Annexin) CHO1 Cortactin CamKinase II Calponin Chondramide Cortexillin CAP Caltropin CH-ILKBP CPb3 Cap100 Calvasculin Ciboulot ... Myosin light chain kinase MAL Mip-90 Myosin Light Chain A1 MARKS MIM MAYP Mycalolide (a macroglide drug) Mayven Myelin basic ... Afadin AFAP-110 Affixin Aginactin AIP1 Aldolase Angiogenin Anilin Annexins Aplyronine Archvillin Arginine kinase Arp2/3 complex ...
... 部分由annexin(英语:Annexin)所介導。基質囊泡從質膜發芽,準確來說是在與細胞外基質相互
2004). „An annexin 1 N-terminal peptide activates leukocytes by triggering different members of the formyl peptide receptor ... Adenozinski (A1, A2A, A2B, A3) • P2Y (1, 2, 4, 5, 6, 8, 9, 10, 11, 12, 13, 14) ...
The expression of Annexin A1 (ANXA1); an anti-inflammatory protein, increases in the adipose tissue in response to obesity and ... Exploring the roles of Annexin A1 in adipocytes using an in vitro model of obesity. Sehar Sajid, Bernard Burke, Christopher Mee ... Exploring the roles of Annexin A1 in adipocytes using an ,em,in vitro,/em, model of obesity (. ...
Annexin A1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene ... ANXA1 (Annexin A1) is a Protein Coding gene. Diseases associated with ANXA1 include Hairy Cell Leukemia and Brain Edema. Among ... annexin A1,calcium and phospholipid binding protein,calpactin 2 (calelectrin),expressed in the supporting cells of the organ of ... Annexin A1-containing extracellular vesicles and polymeric nanoparticles promote epithelial wound repair. (PMID: 25664854) ...
Fingerprint Dive into the research topics of Negative regulatory role of Annexin-A1 in 14-3-3η-mediated glucocorticoid ... Negative regulatory role of Annexin-A1 in 14-3-3η-mediated glucocorticoid receptor transcriptional activation. ...
... Thomas M. Raffay,1 Morgan L. Locy,2 Cynthia ... Expression of lung SCGB and annexin A1 (ANXA1) is persistently altered in CCSP knockout mice suggesting that CCSP indirectly ...
IPR001464. Annexin. IPR018502. Annexin_repeat. IPR018252. Annexin_repeat_CS. IPR037104. Annexin_sf. IPR002388. ANX1. ... IPR001464. Annexin. IPR018502. Annexin_repeat. IPR018252. Annexin_repeat_CS. IPR037104. Annexin_sf. IPR002388. ANX1. ... sp,P24551,ANXA1_RODSP Annexin A1 OS=Rodentia sp. OX=69158 GN=ANXA1 PE=2 SV=3 ... Annexin 1Add BLAST. 61. ,p>This subsection of the Family and Domains section indicates the positions and types of repeated ...
Rabbit polyclonal Annexin A1 antibody validated for WB, IHC, ICC/IF and tested in Human. Immunogen corresponding to recombinant ... Anti-Annexin A1 antibody (ab97485) at 1/1000 dilution + H1299 whole cell lysate at 30 µg. Predicted band size : 39 kDa. ... Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1-198 of Human Annexin A1 ( ... A pair of annexin repeats may form one binding site for calcium and phospholipid. ...
Buy our Human Annexin A1 peptide. Ab47659 is a Synthetic peptide for ab46686. Abcam provides free protocols, tips and expert ... I have purchased Annexin A1 peptide (ab47659) and I would like to know if you could recommend me the best storage buffer for ... A pair of annexin repeats may form one binding site for calcium and phospholipid. ...
... annexin A1), Authors: Flavia Cristina Rodrigues-Lisoni, Tiago Henrique, Eloiza Helena Tajara. Published in: Atlas Genet ... Similarly to other annexins, annexin A1 is characterized by a C-terminal homologous domain with 4 to 8 repeats of 70-75 ... Loss of annexin A1 expression in breast cancer progression.. Cao Y, Li Y, Edelweiss M, Arun B, Rosen D, Resetkova E, Wu Y, Liu ... Annexin A1 expression and its prognostic significance in human breast cancer.. Wang LP, Bi J, Yao C, Xu XD, Li XX, Wang SM, Li ...
Mono-Ubiquitination of Nuclear Annexin A1 and Mutagenesis , IntechOpen, Published on: 2012-08-17. Authors: Fusao Hirata, George ... Native annexin A1 was detected at pI 6.4. The reaction mixture containing SUMOylated annexin A1 showed a new annexin A1 ... when amounts of ubiquitinated annexin A1 were adjusted with the total amounts of annexin A1 (free and ubiquitinated annexin A1 ... 6. Heavy metals and annexin A1. Annexin A1 has 3 different types of Ca2+ binding sites, type II, type III and type III (AB) ( ...
Invitrogen Anti-Annexin A1 Recombinant Polyclonal, Catalog # 711089. Tested in Western Blot (WB) applications. This antibody ... Protein Aliases: Annexin A1; Annexin I; annexin I (lipocortin I); Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; ... Cite Annexin A1 Recombinant Polyclonal Antibody. The following product was used in this experiment: Annexin A1 Recombinant ... A 38 kDa band corresponding to Annexin A1 was observed across cell lines tested. Known quantity of protein samples were ...
Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately ... Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since ... annexin I may have potential anti-inflammatory activity. ...
Annexin A1 and the Resolution of Inflammation: Modulation of Neutrophil Recruitment, Apoptosis, and Clearance. Michelle Amantéa ... Annexin A1 (AnxA1), also known as lipocortin-1, is an endogenous glucocorticoid-regulated protein, which is able to ...
Shop a large selection of products and learn more about Annexin A1 Rabbit anti-Human, Polyclonal, Invitrogen 1 mL; Unconjugated ... Annexin A1, a gene related to phagocytosis, was found to be one of the most highly upregulated genes in hairy cell leukemia. ... ANX1, LPC1, annexin I lipocortin I, annexin-1, calpactin II, calpactin-2, chromobindin-9, lipocortin I, p35, phospholipase A2 ...
Unconjugated Whole IgG Rabbit anti-ANXA1/Annexin A1 Antibody, Affinity Purified suitable for WB, IP, IHC applications. Visit ... ANXA1/Annexin A1 Antibody, A305-235A. ANXA1/Annexin A1 Antibody, A305-235A. ...
We hypothesized that Annexin A1 (ANXA1), present in MSC-EVs, contributed to their therapeutic potential by targeting the ANXA1/ ... Annexin A1 as Neuroprotective Determinant for Blood-Brain Barrier Integrity in Neonatal Hypoxic-Ischemic Encephalopathy Ruth ... We hypothesized that Annexin A1 (ANXA1), present in MSC-EVs, contributed to their therapeutic potential by targeting the ANXA1/ ... Keywords: Annexin A1/Formyl peptide receptor axis; blood-brain barrier; mesenchymal stem cell-derived extracellular vesicles; ...
annexin A1. Synonyms: Anx-1, Anx-A1, C430014K04Rik, Lpc-1, Lpc1. Gene nomenclature, locus information, and GO, OMIM, and PMID ... OMIM: ANNEXIN A1; ANXA1*Gene Ontology: Anxa1 *Mouse Phenome DB: Anxa1 *UCSC: Chr.19:20,373,434-20,390,671(-)*IMPC: Anxa1 ...
... annexin A1-externalizing cells induced chemoattraction of monocytes, which was clearly reduced in annexin A1- or ADAM10- ... Cleavage of Annexin A1 by ADAM10 during Secondary Necrosis Generates a Monocytic "Find-Me" Signal. Karin E. Blume, Szabolcs ... Annexin A1 is an intracellular calcium/phospholipid-binding protein that is involved in membrane organization and the ... As such, annexin A1 promotes the engulfment of dying cells and dampens the postphagocytic production of proinflammatory ...
Annexin A1 counteracts chemokine-induced integrin activation. A and B, Annexin A1 inhibits chemokine-evoked integrin activation ... In vivo delivery of the annexin A1 fragment Ac2-26 reduces atherosclerosis. A-C, Apoe−/−, Apoe−/−Fpr2−/−, and Apoe−/−Anxa1−/− ... Annexin A1-formyl peptide receptor 2 axis prevents arterial myeloid cell recruitment. Apoe−/−, Apoe−/−Fpr2−/−, and Apoe−/−Anxa1 ... Formyl peptide receptor 2 and its ligand annexin A1 protect from atherogenesis. Apoe−/−, Apoe−/−Fpr2−/−, and Apoe−/−Anxa1−/− ...
Annexin A1. By Technical Data. Annexin A1 - Antibodies. Annexin A1 - Polyclonal Antibody - Antibodies. Human - Annexin A1 - ... Annexin A1 - from Sheep - Antibodies. Annexin A1 - for Western blotting - Antibodies. Annexin A1 - for ELISA - Antibodies. ... References to Annexin A1. *Ansari J, Kaur G, Gavins FNE. Therapeutic Potential of Annexin A1 in Ischemia Reperfusion Injury. ... Annexin A1 - for Immunohistochemistry - Antibodies. Annexin A1 - Antigene from E. coli - Antibodies. Polyclonal Antibody - ...
Mass spectrometric analysis identified annexin A1 as a key mediator of keratinocyte death; depletion of annexin A1 by a ... An annexin A1-FPR1 interaction contributes to necroptosis of keratinocytes in severe cutaneous adverse drug reactions ... Annexin A1 secreted from drug-stimulated monocytes contributes to keratinocyte necroptosis in serious drug-related adverse ... Annexin A1 secreted from drug-stimulated monocytes contributes to keratinocyte necroptosis in serious drug-related adverse ...
Open this publication in new window or tab ,,Higher expression of annexin A1 in 1 CD56+ than in CD56-T cells: Potential ... Pro- and anti-inflammatory actions in coronary artery disease: with focus on CD56+ T cells and Annexin A1. Bergström, Ida ... Annexin A1 (AnxA1) is a glucocorticoidinduced protein which may have a key role in the anti-inflammatory response as a mediator ... 3. Higher expression of annexin A1 in 1 CD56+ than in CD56-T cells: Potential implications for coronary artery disease. ...
Reperfusion-induced Myocardial Dysfunction is Prevented by Endogenous Annexin-A1 and Its N-terminal Derived Peptide Ac-ANX-A1 ( ... Reperfusion-induced Myocardial Dysfunction is Prevented by Endogenous Annexin-A1 and Its N-terminal Derived Peptide Ac-ANX-A1 ( ...
Annexin A1 antibody Mouse Monoclonal from Proteintech validated in Western Blot (WB), Immunohistochemistry (IHC), ... WB protocol for Annexin A1 antibody 66344-1-Ig. Download protocol. IHC protocol for Annexin A1 antibody 66344-1-Ig. Download ... Annexin 1, annexin A1, Annexin I, ANX1, ANXA1, Calpactin 2, Calpactin II, Chromobindin 9, Lipocortin I, LPC1, p35 ... Annexin A1 Antibody. Annexin A1 Monoclonal Antibody for IF, IHC, WB, ELISA. ...
Annexin A1- containing extracellular vesicles and polymeric nanoparticles promote epithelial wound repair. In: Journal of ... Annexin A1- containing extracellular vesicles and polymeric nanoparticles promote epithelial wound repair. Journal of Clinical ... Annexin A1- containing extracellular vesicles and polymeric nanoparticles promote epithelial wound repair. / Leoni, Giovanna; ... In this work, we demonstrate that endogenous annexin A1 (ANXA1) is released as a component of extracellular vesicles (EVs) ...
Annexin A1, BRCA1 and BRCA2 mutations, Breast cancer Persistent URL dx.doi.org/10.1186/s12916-015-0392-6, hdl.handle.net/1765/ ... Annexin A1 expression in a pooled breast cancer series: Association with tumor subtypes and prognosis. Publication. Publication ... Background: Annexin A1 (ANXA1) is a protein related with the carcinogenesis process and metastasis formation in many tumors. ... Annexin A1 expression in a pooled breast cancer series: Association with tumor subtypes and prognosis. BMC Medicine. doi: ...
Annexin A1 (ANXA1) Recombinant Protein-NP_001075336.1 (MBS1346876) product datasheet at MyBioSource, Recombinant Proteins ... Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately ... Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since ... annexin I may have potential anti-inflammatory activity. ... ANXA1 recombinant protein :: Annexin A1 (ANXA1) Recombinant ...
RT-PCR revealed mRNA and Western blotting demonstrated protein for the ATP binding cassette A1 (ABCA1) transporter in mouse FS ... Annexin 1 (ANXA1) has a well-demonstrated role in early delayed inhibitory feedback of glucocorticoids in the pituitary. ANXA1 ... Annexin A1 complex mediates oxytocin vesicle transport.. *Vishruti Makani, Rukhsana Sultana, +8 authors Ji-Hyuk Park ... Annexin A1 modulates macula densa function by inhibiting cyclooxygenase 2.. *Stefan Seidel, Hanna Neymeyer, +6 authors Alex ...
In vitro experiments have identified involvement of Annexin A1 (Anx A1) in both these fusion processes. To determine if Anx A1 ... Instead, lack of Anx A1 lowered the proportion of differentiating myoblasts that managed to fuse with the injured myofibers by ... Despite this early slowdown in fusion of Anx A1-/- myoblasts, regeneration caught up at later times post injury. These results ... However, the lack of Anx A1 delayed muscle regeneration after notexin-induced injury. This delay in muscle regeneration was not ...
You are here: Home / Publications / Decreased expression of annexin A1 is correlated with breast cancer development and ... Decreased expression of annexin A1 is correlated with breast cancer development and progression as determined by a tissue ... Annexin A1 (ANXA1) is a calcium- and phospholipid-binding protein and a known mediator of glucocorticoid-regulated inflammatory ...
ANX-A1; ANX1; LPC1; Lipocortin I; Chromobindin-9; Calpactin II; Phospholipase A2 inhibitory protein , Products for research use ... ELISA Kit DIY Materials for Annexin A1 (ANXA1), ... ELISA Kit DIY Materials for Annexin A1 (ANXA1). ANX-A1; ANX1; ... Monoclonal Antibody to Annexin A1 (ANXA1). WB; IHC; ICC; IP.. SEE787Ra. ELISA Kit for Annexin A1 (ANXA1). Enzyme-linked ... Polyclonal Antibody to Annexin A1 (ANXA1). WB; IHC; ICC; IP.. LAE787Ra71. Biotin-Linked Polyclonal Antibody to Annexin A1 ( ...
... * QMRO ... Up-regulation of Annexin-A1 and lipoxin A(4) in individuals with ulcerative colitis may promote mucosal homeostasis.. ... Up-regulation of Annexin-A1 and lipoxin A(4) in individuals with ulcerative colitis may promote mucosal homeostasis. ... The pro-resolution mediators Annexin-A1 (AnxA1) and lipoxin A(4) (LXA(4)) exert counter-regulatory effects on leukocyte ...
Analysis of MOG35-55-induced EAE development in Annexin A1 null mice showed decreased signs of the disease compared to wild ... We have reported novel biological functions for Annexin A1, an effector of endogenous anti-inflammation, to produce positive ... In this study, we investigated the potential modulatory role of Annexin A1 in the development of experimental autoimmune ... Together these findings suggest that Annexin A1 null mice have an impaired capacity to develop EAE. Furthermore strategies ...
  • Schematic representation of annexin protein. (atlasgeneticsoncology.org)
  • The comparison of conserved and variable residues between human annexin 1 protein and annexin protein from different vertebrates shows amino acid identities of 100% (Pan troglodytes), 91% (Canis familiars), 87% (Mus musculus), 89% (Rattus norvegicus) and 77% (Gallus gallus) (Rodrigues-Lisoni et al. (atlasgeneticsoncology.org)
  • Annexin A1 is an intracellular calcium/phospholipid-binding protein that is involved in membrane organization and the regulation of the immune system. (jimmunol.org)
  • Annexin 1-dependent actions of glucocorticoids in the anterior pituitary gland: roles of the N-terminal domain and protein kinase C. (semanticscholar.org)
  • Host: Mouse Isotype: IgG2aκ Immunogen: Human Annexin A4 (NP_001144.1, 1 aa-321 aa) full-length recombinant protein with 6xHis tag. (canvaxbiotech.com)
  • Annexin A1 (ANX-1), a calcium-dependent, phospholipid binding protein, is known to be involved in diverse cellular processes, including regulation of cell growth and differentiation, apoptosis, and inflammation. (elsevier.com)
  • Oliani SMPerreti M Cell localization of the anti-inflammatory protein annexin 1 during experimental inflammatory response. (jamanetwork.com)
  • Moreover, a comparison between plaque-derived SMC isolated from patients with or without recent acute cerebrovascular symptoms uncovered an increase in Annexin A1, an endogenous anti-inflammatory protein, in the asymptomatic group. (ox.ac.uk)
  • The purpose of this study was to investigate the effect of annexin-A1 protein as an endogenous regulator of the organ remote injury induced by intestinal ischemia/reperfusion. (biomedcentral.com)
  • In addition, ANX A1 protein has been identified as a key regulator in some important signaling pathways, which influences cytoskeletal remodeling in many diseases, such as lung cancer, liver cancer, etc. (biomedcentral.com)
  • In this study, we found that the serum from a common bile duct ligation(CBDL) Rat model decreased the expression levels of the ANX A1 mRNA and protein by at least two-fold in human PMVECs. (biomedcentral.com)
  • It harbours binding sites for cytoplasmic protein ligands that can be targeted to membranes through the annexin-core-mediated phospholipid interaction. (nature.com)
  • Also known as Annexin A1 (Annexin I) (Annexin-1) (Calpactin II) (Calpactin-2) (Chromobindin-9) (Lipocortin I) (Lipocortin-like 33 kDa protein) (Phospholipase A2 inhibitory protein) (p35). (mybiosource.com)
  • Annexin A1 is a protein that stimulates the growth of cancer. (24-7pressrelease.com)
  • Description This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. (gentaur.com)
  • The following product was used in this experiment: Annexin A1 Recombinant Polyclonal Antibody from Thermo Fisher Scientific, catalog # 711089, RRID AB_2633117. (thermofisher.com)
  • The antibody was raised in sheep by immunization with the recombinant Human Annexin A1. (biovendor.com)
  • depletion of annexin A1 by a specific antibody diminished supernatant cytotoxicity. (sciencemag.org)
  • Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66344-1-Ig( Annexin A1 antibody at dilution of 1:200 (under 10x lens). (ptglab.com)
  • Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66344-1-Ig( Annexin A1 antibody at dilution of 1:200 (under 40x lens). (ptglab.com)
  • Western Blot: Annexin A3 Antibody (2F3) [NBP2-03373] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Annexin A3 (Right lane) cDNA for 48 hrs and lysed. (novusbio.com)
  • Immunocytochemistry/ Immunofluorescence: Annexin A3 Antibody (2F3) [NBP2-03373] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Annexin A3. (novusbio.com)
  • Immunohistochemistry-Paraffin: Annexin A3 Antibody (2F3) [NBP2-03373] - Staining of paraffin-embedded Human prostate tissue using anti-Annexin A3 mouse monoclonal antibody. (novusbio.com)
  • I have purchased Annexin A1 peptide (ab47659) and I would like to know if you could recommend me the best storage buffer for this product. (abcam.com)
  • Most importantly, we demonstrate that the released peptide and culture supernatants of secondary necrotic, annexin A1-externalizing cells induced chemoattraction of monocytes, which was clearly reduced in annexin A1- or ADAM10-knockdown cells. (jimmunol.org)
  • Thus, altogether our findings indicate that annexin A1 externalization and its proteolytic processing into a chemotactic peptide represent final events during apoptosis, which after the transition to secondary necrosis contribute to the recruitment of monocytes and the prevention of inflammation. (jimmunol.org)
  • The necroptosis-mediating complex of RIP1 and RIP3 was indispensable for SJS/TEN supernatant-induced keratinocyte death, and SJS/TEN keratinocytes expressed abundant formyl peptide receptor 1 (FPR1), the receptor for annexin A1, whereas control keratinocytes did not. (sciencemag.org)
  • Reperfusion-induced Myocardial Dysfunction is Prevented by Endogenous Annexin-A1 and Its N-terminal Derived Peptide Ac-ANX-A1 (2-26). (edu.au)
  • Annexin 1-derived peptide Ac2-26 inhibits eosinophil recruitment in vivo via decreasing prostaglandin D₂. (biomedsearch.com)
  • Previous studies have emphasized that the N-terminal peptide of annexin 1 (peptide Ac2-26) can inhibit mast cell degranulation, antigen-induced eotaxin release as well as the accumulation of both neutrophils and eosinophils in a model of rat pleurisy. (biomedsearch.com)
  • Treatment with annexin-A1 peptidomimetic Ac2-26, reduced the number of neutrophils in the lung tissue and increased its number in the blood vessels, which suggests a regulatory effect of the peptide Ac2-26 in the neutrophil migration. (biomedcentral.com)
  • Post-translational modification plays an essential role in the translocation of annexin A1 from the cytoplasm to the cell surface. (ox.ac.uk)
  • Human Annexin A13 (ANXA13), also known as Annexin XIII or Annexin 13, is a 35 kDa member of the Annexin family (1), which are calcium-dependent phospholipid-binding proteins being preferentially located on the cytosolic face of the plasma membrane. (novusbio.com)
  • This gene encodes a member of the annexin family. (novusbio.com)
  • ANXA10: a member of the annexin family. (mybiosource.com)
  • Decreased expression of annexin A1 is correlated with breast cancer development and progression as determined by a tissue microarray analysis. (nih.gov)
  • it is likely that nuclear annexin A1 is involved in DNA replication, especially DNA damage induced gene mutation, since DNA damage induced mutagenesis plays an important role in tumorigenesis. (intechopen.com)
  • Annexin A1, a gene related to phagocytosis, was found to be one of the most highly upregulated genes in hairy cell leukemia. (fishersci.com)
  • Genetic polymorphism of miRNA-196a and its target gene annexin-A1 expression based on ethnicity in Pakistani female breast cancer patients. (cdc.gov)
  • To evaluate the association of miR-196a rs11614913 C/T genetic variation and its target gene annexin A 1 mRNA expression with breast cancer risk in Pakistani female ethnicities. (cdc.gov)
  • Metaplastic stratified squamous epithelium shows increasing annexin A1 expression from basal cells to superficial cells (hematoxylin-eosin, original magnification ×400). (jamanetwork.com)
  • Lipocortin 1 (annexin 1): a candidate paracrine agent localized in pituitary folliculo-stellate cells. (semanticscholar.org)
  • Goulding NJLuying PJefferiss CMRigby WFCGuyre PM Specific binding of lipocortin 1 (annexin I) to monocytes and neutrophils is decreased in rheumatoid arthritis. (jamanetwork.com)
  • Members of the Annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways (3, 4). (novusbio.com)
  • Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity. (thermofisher.com)
  • We have reported novel biological functions for Annexin A1, an effector of endogenous anti-inflammation, to produce positive actions on the adaptive immune system by reducing the threshold of T cell activation. (biomedcentral.com)
  • Gibbs LCarollo MGDamazo ASOliani SMPerreti M Time-dependent expression of annexin 1 in a model of chronic granulomatous inflammation. (jamanetwork.com)
  • Vishwanatha JKDavis RGRubinstein AIFloreani A Annexin I degradation in bronchoalveolar lavage fluids from healthy smokers: a possible mechanism of inflammation. (jamanetwork.com)
  • It has been attributed an anti-inflammatory role at various control levels, and recently we could show that annexin A1 externalization during secondary necrosis provides an important fail-safe mechanism counteracting inflammatory responses when the timely clearance of apoptotic cells has failed. (jimmunol.org)
  • McArthur S, Cristante E, Paterno M, Christian H, Roncaroli F, Gillies GE,Solito E. Annexin A1: a central player in the anti-inflammatory andneuroprotective role of microglia. (biovendor.com)
  • Anti-inflammatory drugs, eicosanoids and the annexin A1/FPR2 anti-inflammatory system. (semanticscholar.org)
  • In addition to the anti-inflammatory lipid, lipoxin A4, several other endogenous agonists also bind FPR2, including serum amyloid A, glucocorticoid-induced annexin 1, urokinase and its receptor, suggesting that the activation of FPR2 may result in potent pro- or anti-inflammatory responses. (mdpi.com)
  • Roviezzo FGetting SJPaul-Clark MJ et al The annexin-1 knockout mouse: what it tells us about the inflammatory response. (jamanetwork.com)
  • The deletion of Annexin A1 or the blockade of its signaling in SMC resulted in increased cytokine production at baseline and after stimulation with the pro-inflammatory cytokine Tumor Necrosis Factor α. (ox.ac.uk)
  • In summary, our proteomics and biochemical analysis revealed mitochondrial damage in human plaque-derived SMC as well as a role of Annexin A1 in reducing the production of pro-inflammatory mediators in SMC. (ox.ac.uk)
  • Our data suggest that the annexin-A1 peptidomimetic Ac2-26 treatment has a regulatory and protective effect in the intestinal ischemia/reperfusion by attenuation of the leukocyte migration to the lung and induction of the anti-inflammatory cytokine IL-10 release into the plasma. (biomedcentral.com)
  • The anti-inflammatory action of annexin-A1 and its peptidomimetic described here may serve as a basis for future therapeutic approach in mitigating inflammatory processes due to intestinal ischemia/reperfusion. (biomedcentral.com)
  • Extracellular annexin functions that have been substantiated by mouse knockout models are anti-inflammatory and fibrinolytic activities. (nature.com)
  • Exploiting the Annexin A1 pathway for the development of novel anti-inflammatory therapeutics. (semanticscholar.org)
  • Annexin I belongs to a family of Ca (2+) -dependent phospholipid binding proteins that are preferentially located on the cytosolic face of the plasma membrane. (thermofisher.com)
  • Smooth muscle cells in human atherosclerosis: proteomic profiling reveals differences in expression of Annexin A1 and mitochondrial proteins in carotid disease. (ox.ac.uk)
  • We also found the expression of cytoskeletal proteins (Destrin, a1-actin, and a1-tubulin) in PMVECs significantly increased. (biomedcentral.com)
  • These findings suggest that the ANX A1 down-regulation of PMVEC proliferation in the presence of HPS-rat-serum may be the major cause of aberrant dysregulation of cytoskeletal proteins (Destrin, a1-actin, and a1-tubulin) and may, therefore, play a fundamental role in the proliferation and phenotype differentiation of PMVECs in the PVD of HPS. (biomedcentral.com)
  • Annexins are a multigene family of Ca 2+ -regulated proteins that are characterized by a unique Ca 2+ - and membrane-binding module - the annexin core domain. (nature.com)
  • A unique class of these proteins - annexins - can bind to certain membrane phospholipids in a Ca 2+ -dependent manner, providing a link between Ca 2+ signalling and membrane functions. (nature.com)
  • By forming networks on the membrane surface, annexins can function as organizers of membrane domains and membrane-recruitment platforms for proteins with which they interact. (nature.com)
  • Liemann, S. & Lewit-Bentley, A. Annexins: a novel family of calcium- and membrane-binding proteins in search of a function. (nature.com)
  • The expression levels of Hsp90-beta and annexin A1 were examined using immunohistochemistry, in-situ hybridization, and Western blot. (biomedcentral.com)
  • Annexin A1 modulates macula densa function by inhibiting cyclooxygenase 2. (semanticscholar.org)
  • Frey BMReber BFXVishwanath BSEscher GFrey FJ Annexin I modulates cell functions by controlling intracellular calcium release. (jamanetwork.com)
  • Dreier RSchmid KWGerke VRieheman K Differential expression of annexins I, II and IV in human tissues: an immunohistochemical study. (jamanetwork.com)
  • Annexin I has a molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. (thermofisher.com)
  • Swairjo, M. A. & Seaton, B. A. Annexin structure and membrane interactions: a molecular perspective. (nature.com)
  • Immunoaffinity chromatography on a column with immobilized recombinant Human Annexin A1. (biovendor.com)
  • 66344-1-Ig targets Annexin A1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples. (ptglab.com)
  • Amino acids M1 - N346(end) of human Annexin A1. (dundee.ac.uk)
  • Human Annexin A13 shares approximately 86% and 91% aa sequence identity with mouse and dog Annexin A13 respectively. (novusbio.com)
  • We have previously identified the upregulation of annexin A1 and Hsp90-beta in 16 human bronchial epithelial (HBE) cell lines compared with the NCI-A549 and NCI-H446 with two-dimensional liquid chromatography-tandem mass spectrometry (2D-LC-MS/MS). However, the origin and natural expression of the clinical specimens of lung cancer remain unknown. (biomedcentral.com)
  • Crystal structure of human annexin I at 2.5 Å resolution. (nature.com)
  • Mayran NTraverso VMaroux SMassey-Harroche D Cellular and subcellular localizations of annexins I, IV, and VI in lung epithelia. (jamanetwork.com)
  • However, the effect of Hsp90-beta and annexin A1 in lung cancer remains poorly understood. (biomedcentral.com)
  • The expressions of Hsp90-beta and annexin A1 in lung cancer and normal lung specimens were examined, and the relationships with respect to the clinico-pathological features and patient survival in lung cancer were analyzed. (biomedcentral.com)
  • The upregulation of Hsp90-beta and annexin A1 were associated with poor post-surgical survival time and lymphatic metastasis of lung cancer patients. (biomedcentral.com)
  • In the current study, we identified the upregulation of Hsp90-beta and annexin A1 in lung cancer cells, and we further investigated the significance of this upregulation in lung cancer and the potential use of Hsp90-beta and annexin A1 as clinical markers for lung cancer. (biomedcentral.com)
  • Our results show a direct correlation between Annexin A1 expression and severity of EAE. (biomedcentral.com)
  • Furthermore strategies aiming at reducing Annexin A1 functions or expression in T cells might represent a novel therapeutic approach for multiple sclerosis. (biomedcentral.com)
  • Real time PCR analysis of annexinA1 expression in breast cancer samples in different genotypes of miR-196a . (cdc.gov)
  • They identified increased Annexin A1 expression in some of them. (24-7pressrelease.com)
  • In the study, the patients with greater Annexin A1 expression were more likely to have a worse prognosis in all cases, regardless of their age, sex or cancer stage. (24-7pressrelease.com)
  • Annexin A1 secreted from drug-stimulated monocytes contributes to keratinocyte necroptosis in serious drug-related adverse events in skin. (sciencemag.org)
  • 2010). Therefore, nuclear annexin A1 is thought to play an important role in cell proliferation and/or cell transformation. (intechopen.com)
  • Finally, analysis of the T cell recall response in vitro following stimulation with MOG 35-55 showed a decrease proliferation of Annexin A1 null T cells, with a significantly reduced Th1/Th17 phenotype, compared to wild type cells. (biomedcentral.com)
  • Evidence for a role of the adenosine 5'-triphosphate-binding cassette transporter A1 in the externalization of annexin I from pituitary folliculo-stellate cells. (semanticscholar.org)
  • Externalization of annexin I from a folliculo-stellate-like cell line. (semanticscholar.org)
  • The function of the annexin A1 is far to be clear, it has been involved in signal transduction (Alldridge et al. (atlasgeneticsoncology.org)
  • Annexin-A1: a pivotal regulator of the innate and adaptive immune systems. (semanticscholar.org)
  • Our results confirmed the high specificity of annexin A1 and the BRAF V600E mutation as HCL markers. (bvsalud.org)
  • Contains 4 annexin repeats. (abcam.com)
  • A pair of annexin repeats may form one binding site for calcium and phospholipid. (abcam.com)
  • Similarly to other annexins, annexin A1 is characterized by a C-terminal homologous domain with 4 to 8 repeats of 70-75 aminoacids, responsible for calcium and phospholipid binding properties. (atlasgeneticsoncology.org)
  • The C-terminal domain of Annexin A13 is comprised of four 60‑70 aa annexin repeats which form a tightly packed disc known as the Annexin domain. (novusbio.com)
  • Accordingly, we have investigated how annexin A1 in nuclei stimulates DNA damage-induced mutagenesis. (intechopen.com)
  • In mice, the presence of F. nucleatum stimulates Annexin A1 production, which results in a positive feedback loop that attracts more oral bacteria while feeding the cancer cells. (24-7pressrelease.com)
  • Our results demonstrate that a necroptosis pathway, likely mediated by annexin 1 acting through the FPR1 receptor, contributes to SJS/TEN. (sciencemag.org)