A member of the annexin family that is a substrate for a tyrosine kinase, ONCOGENE PROTEIN PP60(V-SRC). Annexin A2 occurs as a 36-KDa monomer and in a 90-KDa complex containing two subunits of annexin A2 and two subunits of S100 FAMILY PROTEIN P11. The monomeric form of annexin A2 was formerly referred to as calpactin I heavy chain.
Protein of the annexin family exhibiting lipid interaction and steroid-inducibility.
A protein of the annexin family isolated from human PLACENTA and other tissues. It inhibits cytosolic PHOSPHOLIPASE A2, and displays anticoagulant activity.
Protein of the annexin family with a probable role in exocytotic and endocytotic membrane events.
Protein of the annexin family originally isolated from the electric organ of the electric ray Torpedo marmorata. It has been found in a wide range of mammalian tissue where it is localized to the apical membrane of polarized EPITHELIAL CELLS.
An annexin family member that plays a role in MEMBRANE FUSION and signaling via VOLTAGE-DEPENDENT CALCIUM CHANNELS.
A protein of the annexin family that catalyzes the conversion of 1-D-inositol 1,2-cyclic phosphate and water to 1-D-myo-inositol 1-phosphate.
Family of calcium- and phospholipid-binding proteins which are structurally related and exhibit immunological cross-reactivity. Each member contains four homologous 70-kDa repeats. The annexins are differentially distributed in vertebrate tissues (and lower eukaryotes) and appear to be involved in MEMBRANE FUSION and SIGNAL TRANSDUCTION.
A family of highly acidic calcium-binding proteins found in large concentration in the brain and believed to be glial in origin. They are also found in other organs in the body. They have in common the EF-hand motif (EF HAND MOTIFS) found on a number of calcium binding proteins. The name of this family derives from the property of being soluble in a 100% saturated ammonium sulfate solution.
Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a serine moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and serine and 2 moles of fatty acids.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Quaternary ammonium analog of ethidium; an intercalating dye with a specific affinity to certain forms of DNA and, used as diiodide, to separate them in density gradients; also forms fluorescent complexes with cholinesterase which it inhibits.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.
Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
A cell line derived from cultured tumor cells.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Extracellular vesicles generated by the shedding of CELL MEMBRANE blebs.
A family of G-protein-coupled receptors that was originally identified by its ability to bind N-formyl peptides such as N-FORMYLMETHIONINE LEUCYL-PHENYLALANINE. Since N-formyl peptides are found in MITOCHONDRIA and BACTERIA, this class of receptors is believed to play a role in mediating cellular responses to cellular damage and bacterial invasion. However, non-formylated peptide ligands have also been found for this receptor class.
Proteins prepared by recombinant DNA technology.
A short pro-domain caspase that plays an effector role in APOPTOSIS. It is activated by INITIATOR CASPASES such as CASPASE 9. Isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Organic compounds that contain technetium as an integral part of the molecule. These compounds are often used as radionuclide imaging agents.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Established cell cultures that have the potential to propagate indefinitely.
A family of intracellular CYSTEINE ENDOPEPTIDASES that play a role in regulating INFLAMMATION and APOPTOSIS. They specifically cleave peptides at a CYSTEINE amino acid that follows an ASPARTIC ACID residue. Caspases are activated by proteolytic cleavage of a precursor form to yield large and small subunits that form the enzyme. Since the cleavage site within precursors matches the specificity of caspases, sequential activation of precursors by activated caspases can occur.
Fluorescent probe capable of being conjugated to tissue and proteins. It is used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.
An in situ method for detecting areas of DNA which are nicked during APOPTOSIS. Terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 prime OH ends of either single- or double-stranded DNA. The terminal deoxynucleotidyl transferase nick end labeling, or TUNEL, assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA FRAGMENTATION.
A product of the lysis of plasminogen (profibrinolysin) by PLASMINOGEN activators. It is composed of two polypeptide chains, light (B) and heavy (A), with a molecular weight of 75,000. It is the major proteolytic enzyme involved in blood clot retraction or the lysis of fibrin and quickly inactivated by antiplasmins.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
A family of freshwater fish comprising the minnows or CARPS.
Chemical compounds which pollute the water of rivers, streams, lakes, the sea, reservoirs, or other bodies of water.
Phospholipoglycoproteins produced in the fat body of egg-laying animals such as non-mammalian VERTEBRATES; ARTHROPODS; and others. Vitellogenins are secreted into the HEMOLYMPH, and taken into the OOCYTES by receptor-mediated ENDOCYTOSIS to form the major yolk proteins, VITELLINS. Vitellogenin production is under the regulation of steroid hormones, such as ESTRADIOL and JUVENILE HORMONES in insects.
A semisynthetic alkylated ESTRADIOL with a 17-alpha-ethinyl substitution. It has high estrogenic potency when administered orally, and is often used as the estrogenic component in ORAL CONTRACEPTIVES.
Infections with viruses of the order NIDOVIRALES. The concept includes ARTERIVIRUS INFECTIONS and CORONAVIRIDAE INFECTIONS.
Compounds that interact with ESTROGEN RECEPTORS in target tissues to bring about the effects similar to those of ESTRADIOL. Estrogens stimulate the female reproductive organs, and the development of secondary female SEX CHARACTERISTICS. Estrogenic chemicals include natural, synthetic, steroidal, or non-steroidal compounds.
A selective aromatase inhibitor effective in the treatment of estrogen-dependent disease including breast cancer.

Down-regulation of microglial cyclo-oxygenase-2 and inducible nitric oxide synthase expression by lipocortin 1. (1/385)

1. Activated microglial cells are believed to play an active role in most brain pathologies, during which they can contribute to host defence and repair but also to the establishment of tissue damage. These actions are largely mediated by microglial secretory products, among which are prostaglandins (PGs) and nitric oxide (NO). 2. The anti-inflammatory protein, lipocortin 1 (LC1) was reported to have neuroprotective action and to be induced by glucocorticoids in several brain structures, with a preferential expression in microglia. In this paper we tested whether the neuroprotective effect of LC1 could be explained by an inhibitory effect on microglial activation. 3. We have previously shown that bacterial endotoxin (LPS) strongly stimulates PGE2 and NO production in rat primary microglial cultures, by inducing the expression of the key enzymes cyclo-oxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), respectively. 4. Dexamethasone (DEX, 1-100 nM) and LC1-derived N-terminus peptide (peptide Ac2-26, 1-100 microg ml(-1)) dose-dependently inhibited the production of both PGE2 and NO from LPS-stimulated microglia. The inhibitory effects of DEX on NO and of the peptide on NO and PGE2 synthesis were partially abrogated by a specific antiserum, raised against the N-terminus of human LC1. The peptide Ac2-26 did not affect arachidonic acid release from control and LPS-stimulated microglial cultures. 5. Western blot experiments showed that the LPS-induced expression of COX-2 and iNOS was effectively down-regulated by DEX (100 nM) and peptide Ac2-26 (100 microg ml(-1)). 6. In conclusion, our findings support the hypothesis that LC1 may foster neuroprotection by limiting microglial activation, through autocrine and paracrine mechanisms.  (+info)

Modulation of cellular annexin I in human leukocytes infiltrating DTH skin reactions. (2/385)

Based on our previous studies showing endogenous annexin I being depleted from migrated neutrophils (PMN) in vitro, we have tested whether the levels of this glucocorticoid-regulated protein in PMN and mononuclear cells (PBMC) were modified after adhesion to endothelial monolayers in vitro and extravasation into skin blisters in vivo. In vitro, annexin I levels were depleted more significantly (-70%) in post-adherent PMNs than in monocytes (-25%) and lymphocytes (-50%, only in the positive fraction). In vivo, a significant time-dependent increase (approximately threefold, P < 0.05) in cell-associated annexin I was measured in PBMCs recovered from the blisters, whereas no significant changes were detected in extravasated PMNs. This was associated with annexin I release in the blister fluids (approximately 35 ng/mL), whereas no detectable protein was found in matched-paired plasmas. In conclusion, we report for the first time an activation of the annexin I pathway during an ongoing experimental inflammatory response in humans, which is differently regulated between PMNs and PBMCs.  (+info)

Inhibitory effect of annexin I on synovial inflammation in rat adjuvant arthritis. (3/385)

OBJECTIVE: Annexin I is an endogenous antiinflammatory mediator, expressed in rheumatoid arthritis (RA) synovium, the contribution of which to autoregulation of the synovial inflammatory response has not been examined in models of RA. We investigated the antiinflammatory role of annexin I in rat adjuvant arthritis. METHODS: Rats with adjuvant-induced arthritis (AIA) were treated with a specific anti-annexin I monoclonal antibody (mAb), isotype control IgG, and/or dexamethasone. Clinical outcomes and synovial synthesis of tumor necrosis factor alpha (TNFalpha), prostaglandin E2 (PGE2), and nitric oxide were examined, and annexin I expression was assessed by flow cytometry and reverse transcription-polymerase chain reaction. RESULTS: Anti-annexin I mAb reversed the effects of dexamethasone on the clinical features of AIA and exacerbated AIA in the absence of exogenous glucocorticoid. Clinical exacerbation of AIA by anti-annexin I mAb was accompanied by significantly increased synovial TNFalpha and PGE2, suggesting that annexin I tonically inhibits the production of these mediators. Anti-annexin I mAb treatment was associated with significantly reduced leukocyte intracellular annexin I, despite increased annexin I messenger RNA expression, consistent with a depletion effect of extracellular mAb via the cell surface. CONCLUSION: Annexin I is a key endogenous inhibitory mediator of arthritis via mechanisms that include inhibition of cytokine and effector molecule production. Moreover, a synthesis-independent depletion of intracellular annexin I by extracellular antibody supports the hypothesis that externalization of annexin I is involved in its mode of action.  (+info)

Annexin I modulates cell functions by controlling intracellular calcium release. (4/385)

Annexin I is an intracellular protein in search of a function. Ex vivo it has calcium- and phospholipid-binding properties. To evaluate its role in vivo, MCF-7 cells were stably transfected with annexin I in sense or antisense orientations. In cells overexpressing annexin I, calcium release was abrogated on stimulation of purinergic or bradykinin receptors, whereas non-transfected cells or cells with down-regulated annexin I released calcium within seconds. Basal calcium and calcium stores were not affected. The impaired calcium release was paralleled by a down-regulation of the activities of phospholipase C, group II phospholipase A2, and E-cadherin with altered adhesion and enhanced tumor growth on soft agar. Significantly smaller tumors, with the histologically most differentiated cells, were observed in nude mice inoculated with cells transfected with the antisense rather than with the sense plasmid. These observations indicate that annexin I modulates cell functions by controlling intracellular calcium release. Frey, B. M., Reber, B. F. X., Vishwanath, B. S., Escher, G., Frey, F. J. Annexin I modulates cell functions by controlling intracellular calcium release.  (+info)

The inhibitory effect of dexamethasone on lymphocyte adhesion molecule expression and intercellular aggregation is not mediated by lipocortin 1. (5/385)

Glucocorticoids exert their anti-inflammatory activity through multiple pathways which include the inhibition of cell adhesion events. The glucocorticoid-induced protein lipocortin 1 (LC1) has reported anti-inflammatory properties and has been proposed as a putative mediator of the anti-inflammatory effects of glucocorticoids. The role of LC1 in mediating the glucocorticoid inhibition of lymphocyte adhesion and cell adhesion molecule (CAM) expression was investigated in vitro using a microaggregation assay, flow cytometry and confocal microscopy. Lymphocytes stimulated for 96 h with plastic-bound OKT3 antibody showed significant increases in LFA-1 and CD2 expression. Dexamethasone (DEX; 10(-6) M) inhibited this increase but the neutralizing anti-LC1 MoAb 1A (5 microg/ml) failed to reverse the DEX effect; neither was purified human LC1 (50 x 10(-9) M) able to inhibit CAM expression. The biological activity of the LC1 was confirmed by its ability to suppress monocyte phagocytosis and respiratory burst in response to bovine serum albumin (BSA)-anti-BSA complexes. OKT3 stimulation of cultured mononuclear cells resulted in intercellular aggregation, scored microscopically using a visual index. This aggregation was completely reversed by 10-6 M DEX but unaffected by LC1 (50 x 10(-9) M). Significant intracellular expression of lymphocyte LC1 was observed using the anti-LC1 MoAb 1B in saponin-permeabilized cells. Distribution of LC1 had a diffuse, cytoplasmic pattern. LC1 expression was reduced following 3 h treatment with 10(-6) M DEX. These findings indicate that the DEX effects on lymphocyte adhesion and CAM expression are not mediated by LC1. Thus the reported in vivo effects of LC1 on leucocyte adhesion and transmigration probably occur through functional/conformation changes of surface CAM, rather than by alteration in expression.  (+info)

The annexin protein lipocortin 1 regulates the MAPK/ERK pathway. (6/385)

Lipocortin 1 (annexin 1) is a calcium- and phospholipid-binding protein that modulates anti-inflammatory responses including those induced by lipopolysaccharide. To investigate the precise role of lipocortin 1 in regulating the lipopolysaccharide-induced signal transduction pathways, we generated stable RAW 264.7 macrophage cell lines expressing decreased and increased lipocortin 1 protein. Several RAW 264.7 clones with increased lipocortin 1 protein levels showed constitutive activation of the mitogen-activated protein kinase extracellular signal-regulated kinase, which was down-regulated following lipopolysaccharide treatment. Conversely, clones with decreased lipocortin 1 protein expression showed prolonged extracellular signal-regulated kinase activity, following lipopolysaccharide activation. Lipocortin 1 specifically regulates the components of the extracellular signal-regulated kinase pathway, since changes in lipocortin 1 protein expression had no affect on the related mitogen-activated protein kinases p38 and c-Jun N-terminal kinase. Lipocortin 1 modulated upstream components of the extracellular signal-regulated kinase pathway and associated with the adaptor protein growth factor binding protein. The downstream consequences of altered extracellular signal-regulated kinase activity were independent of the proinflammatory transcription factor nuclear factor kappa B. These data indicate that lipocortin 1 specifically regulates proximal signaling components of the extracellular signal-regulated kinase signal transduction pathway, resulting in the modulation of biochemical functions in RAW macrophages.  (+info)

Structural basis of the Ca(2+)-dependent association between S100C (S100A11) and its target, the N-terminal part of annexin I. (7/385)

BACKGROUND: S100C (S100A11) is a member of the S100 calcium-binding protein family, the function of which is not yet entirely clear, but may include cytoskeleton assembly and dynamics. S100 proteins consist of two EF-hand calcium-binding motifs, connected by a flexible loop. Like several other members of the family, S100C forms a homodimer. A number of S100 proteins form complexes with annexins, another family of calcium-binding proteins that also bind to phospholipids. Structural studies have been undertaken to understand the basis of these interactions. RESULTS: We have solved the crystal structure of a complex of calcium-loaded S100C with a synthetic peptide that corresponds to the first 14 residues of the annexin I N terminus at 2.3 A resolution. We find a stoichiometry of one peptide per S100C monomer, the entire complex structure consisting of two peptides per S100C dimer. Each peptide, however, interacts with both monomers of the S100C dimer. The two S100C molecules of the dimer are linked by a disulphide bridge. The structure is surprisingly close to that of the p11-annexin II N-terminal peptide complex solved previously. We have performed competition experiments to try to understand the specificity of the S100-annexin interaction. CONCLUSIONS: By solving the structure of a second annexin N terminus-S100 protein complex, we confirmed a novel mode of interaction of S100 proteins with their target peptides; there is a one-to-one stoichiometry, where the dimeric structure of the S100 protein is, nevertheless, essential for complex formation. Our structure can provide a model for a Ca(2+)-regulated annexin I-S100C heterotetramer, possibly involved in crosslinking membrane surfaces or organising membranes during certain fusion events.  (+info)

Detection of annexins I and IV in bronchoalveolar lavage fluids from calves inoculated with bovine herpes virus-1. (8/385)

Annexins are phospholipid-binding proteins and are abundant in the lung. Annexins I and IV, but not II and VI, have been detected in bronchoalveolar lavage (BAL) fluids from calves inoculated with Pasteurella haemolytica, the pathogen for calf pneumonia. In this study, BAL fluids from calves with experimental pneumonia induced by inoculation to right lung lobes of bovine herpes virus-1 (BHV-1), the major viral pathogen for pneumonia, were examined for detection of annexins I and IV. Of 6 calves inoculated with BHV-1, annexins I and IV were coincidentally detected in BAL fluids from right lung lobes of 4 calves, but not in BAL fluids from left lung lobes of 6 inoculated calves or those from left and right lung lobes of 3 control calves. Annexin II and VI were not found in any BAL fluids examined. These results, together with previous findings on calves inoculated with Pasteurella haemolytica, suggest that the release of annexins I and IV onto the alveolar surface is an essential event occurring in response to pulmonary infections of BHIV-1 and Pasteurella haemolytica.  (+info)

Fingerprint Dive into the research topics of Negative regulatory role of Annexin-A1 in 14-3-3η-mediated glucocorticoid receptor transcriptional activation. Together they form a unique fingerprint. ...
Annexin 1 is an anti-inflammatory protein that plays a key role in innate immunity by modulating the activation of several types of cells, including neutrophils. Here we have developed a cleavage assay using tagged annexin 1 and observed marked activity in the membrane fraction of activated neutrophils. A combination of inhibitors, transfected cells, and proteomic analyses allowed us to identify proteinase 3 as the main enzyme responsible for this cleavage in the N terminus region of the protein, at least in the context of neutrophil activation. Because annexin 1 is an important endogenous anti-inflammatory mediator, blocking its cleavage by proteinase 3 would augment its homeostatic pro-resolving actions and could represent an opportunity for innovative anti-inflammatory drug discovery.. ...
Professor Morands is a physician-scientist whose key interests are clinical outcome measurement and biological profiling in SLE, and the actions of glucocorticoid induced proteins on the immune system. Read more at https://www.monash.edu/medicine/scs/research/cid/rheumatology/researchers/eric-morand His group has pioneered the development of treat-to-target outcome measures in SLE, via the AsiaPacific Lupus Collaboration, which has over 1800 patients under study. Biomarker studies in SLE are also a major interest, with 000s of samples linked to longitudinal clinical data archived in the Australian Lupus Registry & Biobank, headquartered at Monash. His lab discovered the role in rheumatic disease of multiple important glucocorticoid-regulated proteins. Most recently his group defined the role of GILZ in RA, Th17 pathways, B cell activation, and lupus, and are working on exploiting its actions to develop a safe glucocorticoid mimic. His lab also works on innate immunity including the actions of ...
Bird diseases can have a vast impact on humans, so understanding their immune systems can be a benefit for people. An important element in the immune system of
Introduction: Adipose tissue oxygen consumption and blood flow are altered in obesity inducing local tissue hypoxia. Hypoxia affects several biological functions including adipogenesis, inflammation, insulin resistance and metabolism affecting the overall degree of adiposity. The expression of Annexin A1 (ANXA1); an anti-inflammatory protein, increases in the adipose tissue in response to obesity and further increases in the subcutaneous adipose tissue of old obese individuals compared to young obese individuals however, the roles of ANXA1 in the adipose tissue or obesity are unclear.. Aim: The aim of this study was to investigate the potential roles of ANXA1 in adipocytes using an in vitro model of obesity.. Methods: Differentiated SGBS cells were treated with and without 10 μM AC2-26 peptide and incubated in hypoxia (1% O2) for 24 hours to achieve obesogenic conditions. Gene expressions were analysed via RT-qPCR and normalised against GAPDH. Statistical analysis was preformed using GraphPad ...
TY - JOUR. T1 - Annexin A2. T2 - Its molecular regulation and cellular expression in cancer development. AU - Wang, Chi Yun. AU - Lin, Chiou Feng. PY - 2014. Y1 - 2014. N2 - Annexin A2 (ANXA2) orchestrates multiple biologic processes and clinical associations, especially in cancer progression. The structure of ANXA2 affects its cellular localization and function. However, posttranslational modification and protease-mediated N-terminal cleavage also play critical roles in regulating ANXA2. ANXA2 expression levels vary among different types of cancers. With some cancers, ANXA2 can be used for the detection and diagnosis of cancer and for monitoring cancer progression. ANXA2 is also required for drug-resistance. This review discusses the feasibility of ANXA2 which is active in cancer development and can be a therapeutic target in cancer management.. AB - Annexin A2 (ANXA2) orchestrates multiple biologic processes and clinical associations, especially in cancer progression. The structure of ANXA2 ...
Rationale:. The investigators hypothesize that EPO protects against apoptosis after acute ischemia in man and that it is detectable using the annexin-A5 model.. Objective:. Does infusion of a single dose of Epoetin Alfa, a short-acting EPO, protect against apoptosis in man after acute ischemia?. Study design:. A double blinded randomised cross-over study.. Study population:. 12 Healthy male volunteers, between 18 and 40 years old.. Intervention:. All 12 volunteers will receive a single dose of EPO and placebo in a randomized order. A six week wash-out period is obtained in order to avoid interference of both treatments.. Main study parameters/endpoints:. The percentage of difference between radioactivity (quantified as counts per pixel) of the experimental and control thenar muscle at one and four hours after reperfusion. ...
A small, external bioreactor holding human cells pumped out an anti-inflammatory protein to prevent organ damage and other complications in a rat with acute inflammation caused by bacterial products in a model of sepsis, ...
Rabbit polyclonal Annexin-2/ANXA2 (phospho S26) antibody. Validated in WB and tested in Human. Immunogen corresponding to synthetic peptide.
Complete information for ANXA5 gene (Protein Coding), Annexin A5, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Complete information for ANXA1 gene (Protein Coding), Annexin A1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
New research from University of London has revealed that the female sex hormone estrogen may be able to keep the bodys immune systems in check, thus helping to protect women from cardiovascular disease.. The study states that estrogen works on white blood cells so that they do not stick to the insides of blood vessels - a process known to cause dangerous blockages.. The findings of this study might successfully explain why men seem to be more affected by cardiovascular disease than women, and why women are at a higher risk post-menopause.. The research consisted of a comparison between blood samples from pre-menopausal women and men. The blood cells from pre-menopausal women were found to have high levels of annexin-A1 on the surface of the white blood cells. The estrogen moves the annexin-A1 from storage within the white blood cells to the surface. This is what prevents sticking of the cells to the walls of blood vessels, which causes vascular damage.. Image: Shutterstock. ...
Human Annexin A2 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human Annexin A2.
Annexin A2 is a calcium-dependent, phospholipid-binding protein found on many cell types. It consists of a short hydrophobic tail (Ser2-Asn32), which dictates its function, and a core domain (Phe33-Asp339), which is involved in phospholipid binding. Annexin A2 has been implicated in a number of biochemical processes, including cell proliferation, foetal immune tolerance, ion-channel activation, cell-cell interactions and the bridging of membranes. Annexin A2 is reported to be a powerful activator of plasminogen and, therefore, is implicated in many normal and pathological processes such as haemostasis and metastasis. Myeloid cell lines are used, extensively, to study many aspects of cellular proliferation, differentiation and function. In the present study, we have used flow cytometry and real-time PCR to investigate the role of annexin A2 expression in the proliferation and differentiation of a number of myeloid cell lines. The results demonstrated that annexin A2 expression was affected when ...
In our hands, the trypsin actually reduced the Annexin staining presumably by stripping off all the phosphotidyl-serine on the surface along with the surface proteins (adhesion molecules). The only time we have seen enhanced annexin is when the cells suffer enough trauma in harvesting that the membrane is not intact and the Annexin gets inside the cell and attaches to the ps on the inner membrane. Julie At 02:11 PM 8/18/98, you wrote: , ,I have a client who is staining with Annexin-FITC. They are looking at ,cell lines and are using trypsin to get the cells off the flasks. We are ,seeing what we think is an exagerated Annexin response. Does anyone out ,there have any feedback on how trypsin affects Annexin staining? Any ,experience and or information about this will be greatly appreciated. , Thanks in advance, , Joan Kalnitsky ,Joan Kalnitsky ,Flow Cytometry Laboratory ,VMRCVM ,(540) 231-4115 ,FAX 540-231-7367 , , It is better to serve than to receive , B. Borg , , , , , ...
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The Annexin family of calcium-binding proteins is composed of al least thirteen mammalian genes (Annexin A1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. The Annexin family
Non-structural protein 1 (NS1) is a multifunctional protein and a crucial regulatory factor in the replication and pathogenesis of avian influenza virus (AIV). Studies have shown that NS1 can interact with a variety of host proteins to modulate the viral life cycle. We previously generated a monoclonal antibody against NS1 protein; In the current research study, using this antibody, we immunoprecipitated host proteins that interact with NS1 to better understand the roles played by NS1 in communications between virus and host. Co-immunoprecipitation experiments identified annexin A2 (ANXA2) as a target molecule interacting with NS1. Results from confocal laser scanning microscopy indicated that NS1 co-localized with ANXA2 in the cell cytoplasm. Overexpression of ANXA2 significantly increased the titer of H5N1 subtype HPAIV, whereas siRNA-mediated knockdown of ANXA2 markedly inhibited the expression of viral proteins and reduced the progeny virus titer. Our results indicate that ANXA2 interacts with NS1
Proteolytic cleavage of haemagglutinin (HA) is essential for the infectivity of influenza A viruses (IAVs). This is usually mediated by trypsin-like proteases present in the respiratory tract. However, the ability to use plasminogen (PLG) as an alternative protease may contribute to pathogenesis of IAV infections and virus replication outside the respiratory tract. It was demonstrated previously that neuraminidase (NA) of the IAV strain A/WSN/33 can sequester PLG, allowing this virus to replicate in a PLG-dependent fashion. However, PLG also promotes replication of other IAVs, although its mode of action is poorly understood. Here, using NA-deficient viruses, we demonstrate that NA is not required for the binding of PLG and subsequent cleavage of HA. However, we demonstrate that the cellular protein annexin 2 (A2) can bind PLG and contributes to PLG-dependent cleavage of HA and subsequent IAV replication. Collectively, these results indicate that PLG promotes IAV replication in an A2-dependent fashion
We have shown that PPAR-α agonists exert rapid and profound antinociceptive effects in animal models of acute, persistent inflammatory, and neuropathic pain, which are contingent on PPAR-α expression. These effects are comparable in time course and magnitude to those elicited by the clinically used analgesic gabapentin and are not associated with the development of tolerance. Thus, our results reveal a previously unsuspected role of PPAR-α in pain modulation and suggest that this nuclear receptor may provide a novel target for broad-spectrum analgesic drugs.. PPAR-α regulates systemic inflammation by inducing the expression of anti-inflammatory proteins, such as IκB-α, repressing the expression of proinflammatory proteins, such as tumor necrosis factor-α, and limiting the recruitment of immune cells to inflammation sites (Kostadinova et al., 2005). This array of effects is mediated through two separate but converging mechanisms-the induction of responsive target genes and the inhibition ...
Tromp AT, van Strijp JAG. Studying Staphylococcal Leukocidins: A Challenging Endeavor. Front Microbiol. 2020 Apr 15;11:611. doi: 10.3389/fmicb.2020.00611 (Pubmed: 32351474). Tromp AT, Zhao Y, Jongerius I, Heezius ECJM, Abrial P, Ruyken M, van Strijp JAG, de Haas CJC, Spaan AN, van Kessel KPM, Henry T, Haas PA. Pre-existing antibody-mediated adverse effects prevent the clinical development of a bacterial anti-inflammatory protein. Dis Model Mech. 2020 Sep 28;13(9):dmm045534. doi: 10.1242/dmm.045534 (Pubmed: 32471891). Tromp AT, Van Gent M, Jansen JP, Scheepmaker LM, Velthuizen A, De Haas CJC, Van Kessel KPM, Bardoel BW, Boettcher M, McManus MT, Van Strijp JAG, Lebbink RJ, Haas PA, Spaan AN. Host-Receptor Post-Translational Modifications Refine Staphylococcal Leukocidin Cytotoxicity. Toxins (Basel). 2020 Feb 6;12(2):106. doi: 10.3390/toxins12020106. (Pubmed: 32041354). Zhao Y, van Woudenbergh E, Zhu J, Heck AJR, van Kessel KPM, de Haas CJC, Aerts PC, van Strijp JAG, McCarthy AJ. The Orphan ...
This graph shows the total number of publications written about Annexin A3 by people in this website by year, and whether Annexin A3 was a major or minor topic of these publications ...
Richter-Helm BioLogics will help Athera Biotechnologies with development and manufacturing of its cardiovascular product. The recombinant protein Annexin A5 is intended for the treatment of patients with acute coronary syndrome who are at risk for myocardial infarction. ...
Annexin A11 Antibody 10479-2-AP has been identified with IF, IHC, IP, WB, ELISA. 10479-2-AP detected 56-60 kDa band in HeLa cells with 1:500-1:2000 dilution...
购买我们的重组人Annexin A3蛋白。Ab92929为全长蛋白,在大肠杆菌中生产并经过Western blot, SDS-PAGE实验验证。Abcam提供免费的实验方案,操作技巧及专业的支持。
TY - JOUR. T1 - The membrane phospholipid binding protein annexin A2 promotes phagocytosis and nonlytic exocytosis of cryptococcus neoformans and impacts survival in fungal infection. AU - Stukes, Sabriya. AU - Coelho, Carolina. AU - Rivera, Johanna. AU - Jedlicka, Anne E.. AU - Hajjar, Katherine A.. AU - Casadevall, Arturo. PY - 2016/8/15. Y1 - 2016/8/15. N2 - Cryptococcus neoformans is a fungal pathogen with a unique intracellular pathogenic strategy that includes nonlytic exocytosis, a phenomenon whereby fungal cells are expunged from macrophages without lysing the host cell. The exact mechanism and specific proteins involved in this process have yet to be completely defined. Using murine macrophages deficient in the membrane phospholipid binding protein, annexin A2 (ANXA2), we observed a significant decrease in both phagocytosis of yeast cells and the frequency of nonlytic exocytosis. Cryptococcal cells isolated from Anxa2-deficient (Anxa2-/-) bone marrow-derived macrophages and lung ...
The inflammatory process has been described as a crucial mechanism in the pathophysiology of temporal lobe epilepsy. The anti-inflammatory protein annexin A1 (ANXA1) represents an interesting target in the regulation of neuroinflammation through the inhibition of leukocyte transmigration and the release of proinflammatory mediators. In this study, the role of the ANXA1-derived peptide Ac2-26 in an experimental model of status epilepticus (SE) was evaluated. Male Wistar rats were divided into Naive, Sham, SE and SE+Ac2-26 groups, and SE was induced by intrahippocampal injection of pilocarpine. In Sham animals, saline was applied into the hippocampus, and Naive rats were only handled. Three doses of Ac2-26 (1 mg/kg) were administered intraperitoneally (i.p.) after 2, 8 and 14 h of SE induction. Finally, 24 h after the experiment-onset, rats were euthanized for analyses of neuronal lesion and inflammation. Pilocarpine induced generalised SE in all animals, causing neuronal damage, and systemic treatment
Using biochemical, epifluorescence and electron microscopic techniques in a U937 model system, we investigated the effect of anti-allergic drugs di-sodium cromoglycate and sodium nedocromil on the trafficking and release of the anti-inflammatory protein Annexin-A1 (Anx-A1) when this was triggered by glucocorticoid (GC) treatment. GCs alone produced a rapid (within 5min) concentration-dependent activation of PKCalpha/beta (Protein Kinase C; EC 2.7.11.13) and phosphorylation of Anx-A1 on Ser(27). Both phosphoproteins accumulated at the plasma membrane and Anx-A1 was subsequently externalised thereby inhibiting thromboxane (Tx) B(2) generation. When administered alone, cromoglycate or nedocromil had little effect on this pathway however, in the presence of a fixed sub-maximal concentration of GCs, increasing amounts of the cromoglycate-like drugs caused a striking concentration-dependent enhancement of Anx-A1 and PKCalpha/beta phosphorylation, membrane recruitment and Anx-A1 release from cells ...
The role of the calcium- and phospholipid-binding protein annexin I (ANXA1) in cell cycle regulation has been investigated in estrogen receptor (ER)-positive MCF-7 and ER-negative MDA-MB-231 breast tumor cell lines. In MCF-7 cells, ANXA1-targeting small interfering RNA (siRNA) reduced ANXA1 mRNA and protein levels and attenuated cell proliferation induced by FCS, estradiol, or epidermal growth factor. Well-characterized agonists for the known ANXA1 receptor, FPR2, including the ANXA1 N-terminal proteolytic product ANXA1(2-26), lipoxin A(4) (LXA(4)), and the synthetic peptide, Trp-Lys-Tyr-Met-Val-D-Met (WKYMVm), stimulated proliferation of MCF-7 and MDA-MB-231 cells that was attenuated by incubation with FPR2 antagonists WRW(4) (1 μM) or Boc2 (100 nM) or by siRNA against FPR2. FCS-induced mitogenic responses were attenuated by each of the FPR antagonists and by siRNA against FPR2 and, to a lesser extent, FPR1. LXA(4) increased phosphorylation of Akt, p70(S6K) but not ERK1/2. Increases in cyclin ...
Mouse anti Human Annexin A3 antibody, clone 4F1 recognizes human annexin A3, also known as Annexin III, Inositol 1,2-cyclic phosphate 2-ph
Annexin A1 is an intracellular calcium/phospholipid-binding protein that is involved in membrane organization and the regulation of the immune system. It has been attributed an anti-inflammatory role at various control levels, and recently we could show that annexin A1 externalization during secondary necrosis provides an important fail-safe mechanism counteracting inflammatory responses when the timely clearance of apoptotic cells has failed. As such, annexin A1 promotes the engulfment of dying cells and dampens the postphagocytic production of proinflammatory cytokines. In our current follow-up study, we report that exposure of annexin A1 during secondary necrosis coincided with proteolytic processing within its unique N-terminal domain by ADAM10. Most importantly, we demonstrate that the released peptide and culture supernatants of secondary necrotic, annexin A1-externalizing cells induced chemoattraction of monocytes, which was clearly reduced in annexin A1- or ADAM10-knockdown cells. Thus, ...
Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. The alternative splicing event is tissue specific and the mRNA containing the cassette exon is prevalent in brain, heart and skeletal muscle. The transcripts also differ in their 3-non coding regions by the use of two alternative poly(A) signals. Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, ...
Early in the apoptosis process, phosphatidylserine (PS) becomes exposed on the cell surface by flipping from the inner to outer leaflet of the cytoplasmic membrane. This event is thought to be important for macrophage recognition of cells undergoing apoptosis, thus allowing the cells to be rapidly phagocytosed. The binding of Annexin V to phosphatidylserine is calcium-dependent, reversible, but very tight, with a Kd of approximately 5 x 10-10 M. At low PS concentration, a binding ratio of 8 annexins to one PS has been reported. These features make Annexin V conjugates ideal for identifying membrane changes associated with apoptosis, by using either flow cytometric or in situ labeling methods. TACS® Annexin V kits are supplied with an Annexin V conjugate, an optimized binding buffer and propidium iodide. Cells are harvested and washed, then incubated with an Annexin V conjugate in binding buffer, for 15 minutes at room temperature prior to direct analysis. Alternatively, a secondary streptavidin ...
Purpose: : To examine the role of annexin 2 in the formation and internalisation of phagosomes in retinal pigment epithelial (RPE) cells. Methods: : Human ARPE19 cells were cultured on the glass inserts of Matek dishes for immunofluorescence and live imaging, or plastic Nunc dishes for biochemical analysis. In some studies ARPE19 cells were exposed to siRNA for annexin 2 for three days prior to experimentation. Outer segments were prepared from porcine retina using standard protocols (typically 50 - 100 eyes per prep), and either used fresh in biochemical experiments, or labelled with an appropriate fluorescent marker prior to imaging experiments. Results: : Depletion of annexin 2 using siRNA led to a significant quantitative reduction in the phagocytosis of rod outer segments in cultured RPE cells. In cells expressing annexin 2, imaged by confocal microscopy, a striking enrichment of annexin 2 and F-actin was observed at the phagocytic cup immediately upon engagement of the apical cell surface ...
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis. Alternatively spliced transcript variants have been described. [provided by RefSeq, Aug 2010 ...
The annexin superfamily is comprised of a range of Ca2+ dependent phospholipid binding proteins. Each of these proteins are metamorphic, which means that they possesses the capacity to dynamically and reversibly refold themselves into two or more stable conformations. Annexin A5 (ANXA5) has been linked with a variety of cellular functions including: endocytosis, Ca2+ transfer, anticoagulation, membrane repair and exocytosis. Although this protein has been characterised as having pH-dependent and Ca2+-dependent phosphatidylserine (PS) binding the full extent of these mechanisms are not understood.. In this study the changes in ANXA5 conformation as it transitions through its binding states was investigated. Recombinant human ANXA5 was produced in E. coli and subjected to analysis by circular dichroism (CD) and Förster resonance energy transfer (FRET). This revealed that the Ca2+ driven metamorphic refolding of ANXA5 involves minor adjustments of the protein to facilitate trimerisation upon PS ...
The ubiquitin E3 protein ligase Nedd4-2 is a physiological regulator of the epithelial sodium channel ENaC, which is essential for transepithelial Na+ transport and is linked to Liddles syndrome, an autosomal dominant disorder of human salt-sensitive hypertension. Nedd4-2 function is negatively regulated by phosphorylation via a serum- and glucocorticoid-inducible protein kinase (Sgk1), which serves as a mechanism to inhibit the ubiquitination-dependent degradation of ENaC. We report here that 14-3-3 proteins participate in this regulatory process through a direct interaction with a phosphorylated form of human Nedd4-2 (a human gene product of KIAA0439, termed hNedd4-2). The interaction is dependent on Sgk1-catalyzed phosphorylation of hNedd4-2 at Ser-468. We found that this interaction preserved the activity of the Sgk1-stimulated ENaC-dependent Na+ current while disrupting the interaction decreased ENaC density on the Xenopus laevis oocytes surface possibly by enhancing Nedd4-2-mediated ...
|p|Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry
Analysis of lymphocytes in patients with Plasmodium vivax malaria and its relation to the annexin-A1 and IL-10: BackgroundMalaria is the most prevalent parasiti
Annexin V Binding Buffer - Annexin V Binding Buffer has been formulated for flow cytometric labeling of apoptotic cells with Annexin V reagents.  The buffer is provided as a ready-to-use solution..
Invitrogen™ eBioscience™ Annexin V, Recombinant Protein Unlabeled; 300μg Invitrogen™ eBioscience™ Annexin V, Recombinant Protein...
In the present study, we show that both CRP and annexin A5 specifically bind to oxidized LDL. Via coupling to Fcγ receptors on macrophages and independent of complement, CRP subsequently enhances the association of oxidized LDL to macrophages. Enhanced association of oxidized LDL to macrophages via CRP-Fcγ receptor interaction may lead to enhanced macrophage uptake of oxidized LDL. Moreover, we show that annexin A5 binds to a site at oxidized LDL different from the CRP binding site and that annexin A5 does not antagonize the CRP effect.. Previously, Chang et al also observed specific binding of CRP to oxidized LDL,7 characterized by inhibition by whole molecules as well as F(ab′)2 fragments of EO6, an IgM antibody that binds specifically to the phosphorylcholine head group of oxidized phospholipids but not to the same moiety on nonoxidized phospholipids.26 In the present study, for characterization of binding sites, we used the monoclonal antibody DLH3 (also IgM), which recognizes an epitope ...
Annexin A6 (AnxA6) has been implicated in the regulation of endo-/exocytic pathways, cholesterol transport, and the formation of multifactorial signaling complexes in many different cell types. More recently, AnxA6 has also been linked to triglyceride storage in adipocytes. Here we investigated the potential role of AnxA6 in fatty acid (FA) induced lipid droplet (LD) formation in hepatocytes. AnxA6 was associated with LD from rat liver and HuH7 hepatocytes. In oleic acid (OA) -loaded HuH7 cells, substantial amounts of AnxA6 bound to LD in a Ca2+-independent manner. Remarkably, stable or transient AnxA6 overexpression in HuH7 cells led to elevated LD numbers/size and neutral lipid staining under control conditions as well as after OA loading compared to controls. In contrast, overexpression of AnxAl, AnxA2 and AnxA8 did not impact on OA-induced lipid accumulation. On the other hand, incubation of AnxA6-depleted HuH7 cells or primary hepatocytes from AnxA6 KO-mice with OA led to reduced FA ...
Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 has 45 to 59% identity with other members of its family and shares a similar size and exon-intron organization. Isolated from human placenta, ANX4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells ...
Annexin A2 (AnxA2) is a multi-functional and -compartmental protein whose subcellular localisation and functions are tightly regulated by its post-translational modifications. AnxA2 and its Tyr23-phosphorylated form (pTyr23AnxA2) are involved in malignant cell transformation, metastasis and angiogenesis. Here, we show that H2O2 exerts rapid, simultaneous and opposite effects on the Tyr23 phosphorylation status of AnxA2 in two distinct compartments of rat pheochromocytoma (PC12) cells. Reactive oxygen species induce dephosphorylation of pTyr23AnxA2 located in the PML bodies of the nucleus, whereas AnxA2 associated with F-actin at the cell cortex is Tyr23 phosphorylated. The H2O2-induced responses in both compartments are transient and the pTyr23AnxA2 accumulating at the cell cortex is subsequently incorporated into vesicles and then released to the extracellular space. Blocking nuclear export by leptomycin B does not affect the nuclear pool of pTyr23AnxA2, but increases the amount of total AnxA2 ...
WBC treatments result is a very different response from the body. Three minutes of extreme, dry cold reaches only the top skin layers and receptors by 30 to 50 degrees, causing the brain to restrict blood flow to an internal cycle. WBC causes the body to release anti-inflammatory proteins and endorphins resulting in oxygenated blood. In an ice bath, fifteen minutes of cold water causes the body to move blood to the extremities and results in a lowered core body temperature. Ice baths do no oxygenate the blood ...
Annexin II antibody (annexin A2) for ICC/IF, IHC-P, IP, RipA, WB. Anti-Annexin II pAb (GTX32446) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
The atherosclerotic process is considered to be driven by an imbalance between proand anti-inflammatory actions. Still, the inflammatory state in patients with coronary artery disease (CAD) remains to be clarified. Annexin A1 (AnxA1) is a glucocorticoidinduced protein which may have a key role in the anti-inflammatory response as a mediator of glucocorticoid effects.. The general aim of this thesis was to deepen the knowledge of pro- and antiinflammatory mechanisms in CAD via phenotypic assessments of immune cell subsets, in particular CD56+ T cells, and exploration of AnxA1. The long-term goal is to reveal basic mechanisms that will lead to the development of biomarkers, which may be used for individualized treatment and monitoring.. The AnxA1 protein was constitutively expressed in both neutrophils and peripheral blood mononuclear cells (PBMCs). However, it varied considerably across PBMC subsets, being most abundantly expressed in monocytes. The AnxA1 expression was also higher in CD56+ T ...
Annexin A2兔多克隆抗体(ab90643)可与人样本反应并经WB, IHC实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
Annexin V is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Annexin V is a monomer, binds ATRX and EIF5B, and interacts with hepatitis B virus (HBV ...
ANXA2, a member of the annexin family, is a calcium-dependent phospholipid-binding protein that plays a role in the regulation of cellular growth and in signal transduction pathways. Its affinity for calcium is greatly enhanced by anionic phospholipids, and it binds two calcium ions with high affinity. ANXA2 may be involved in heat-stress response. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. It may also cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis.
Order monoclonal and polyclonal Annexin A13 antibodies for many applications. Selected quality suppliers for anti-Annexin A13 antibodies.
Buy ANXA2 recombinant protein, Annexin A2 Recombinant Protein-NP_001002857 (MBS203687) product datasheet at MyBioSource, Recombinant Proteins. Application: SDS-PAGE
SCAN HISTORY OWL11_0 2 100 NSINGLE OWL17_1 2 110 NSINGLE OWL18_0 1 115 NSINGLE OWL19_1 1 125 NSINGLE OWL21_1 1 125 NSINGLE OWL26_0 1 125 NSINGLE SPTR37_9f 2 237 NSINGLE INITIAL MOTIF SETS ANNEXIN1 Length of motif = 23 Motif number = 1 Annexin motif I - 1 PCODE ST INT KTKGVDEVTIVNILTNRSNAQRQ LUHU36 46 46 KTKGVDEVTIINILTNRSNEQRQ ANX2_CHICK 46 46 TVKGVDEATIIDILTKRNNAQRQ ANX1_CAVCU 56 56 MVKGVDEATIIDILTKRNNAQRQ LUHU 55 55 KGIGTDEATIIDIVTHRSNAQRQ ANX6_MOUSE 376 376 KGMGTDEETILKILTSRNNAQRQ ANX5_CHICK 29 29 KGIGTNEQAIIDVLTKRSNTQRQ ANX8_HUMAN 35 35 KGFGTDEQEIIDVLVGRSNQQRQ DROANNX 29 29 RGIGTDEKMLISILTERSNAQRQ ANX3_HUMAN 32 32 KGLGTDEDAIINVLAYRSTAQRQ ANX4_BOVIN 27 27 KGFGTDEQAIVDVVANRSNDQRQ HUMSNEXIN 177 177 TAKGVDEATIIDIMTTRTNAQRP ANX1_COLLI 51 51 ANNEXIN2 Length of motif = 17 Motif number = 2 Annexin motif II - 1 PCODE ST INT LKSALSGHLETVILGLL LUHU36 86 17 LKSALSGHLEAVILGLL ANX2_CHICK 86 17 LKKALTGHLEEVVLALL ANX1_CAVCU 96 17 LKKALTGHLEEVVLALL LUHU 95 17 LKSEISGDLARLILGLM ANX6_MOUSE 416 17 ...
1HVD: Structural and electrophysiological analysis of annexin V mutants. Mutagenesis of human annexin V, an in vitro voltage-gated calcium channel, provides information about the structural features of the ion pathway, the voltage sensor and the ion selectivity filter
BioAssay record AID 502910 submitted by ChEMBL: Induction of annexin 2-mediated cytoskeleton aggregate accumulation in human HepG2 cells at 4 uM after 2 hrs by confocal laser scanning microscopy.
This highly specific Annexin A1 antibody (clone 6E4/3) is suitable for use in FACS, IHC-P, and is guaranteed to work as stated on the product data sheet. | V3741
Bachem offers H-3196 Annexin A1 (1-25) (dephosphorylated) (human) for your research. Find all specific details here. Find product specific information including available pack sizes, CAS, detailed description and references here.
Mouse Monoclonal Anti-Annexin A3 Antibody (2F3). Validated: WB, ICC/IF, IHC, IHC-P. Tested Reactivity: Human, Monkey. 100% Guaranteed.
Purified Recombinant Mouse Anxa1 Protein, His-tagged from Creative Biomart. Recombinant Mouse Anxa1 Protein, His-tagged can be used for research.
Annexin A2 is a calcium regulated protein involved in membrane binding and actin polymerization. In this paper, Kevin Moreau and colleagues identified Annexin A2 as a…. ...
It has been reported that Annexin A2 (ANXA2) is up-regulated in hepatocellular carcinoma (HCC), but the roles of ANXA2 in the migration and invasion of HCC ...
Expression of ANXA6 (ANX6) in cancer tissue. The cancer tissue page shows antibody staining of the protein in 20 different cancers.
Human ANXA2 full-length ORF ( AAH09564.1, 1 a.a. - 339 a.a.) recombinant protein with GST-tag at N-terminal. (H00000302-P01) - Products - Abnova
Main / Clearance & Specials / Effet zoloft 50 Le traitement par la sertraline doit être débuté à la dose de 50 mg/jour. Trouble panique, ESPT et Trouble Anxiété Sociale. Le traitement sera débuté à la dose de 25 mg/jour. Après une semaine, la dose sera augmentée à 50 mg une fois par jour. Ce schéma posologique a montré quil réduisait la fréquence des effets Prix de vente TTC‎: ‎1,95 €. Si vous arrêtez de prendre ZOLOFT 50 mg, gélule»). Une augmentation du risque de fractures a été observée chez les patients prenant ce type de médicaments. Déclaration des effets secondaires. Si vous ressentez un quelconque effet indésirable, parlez-en à votre médecin ou votre ‎Description médicament · ‎Informations · ‎Utilisation.. Mit Hurghada Reisen unternehmen Sie einen sehr intressanten Tagesausflug von Serotonin Bay nach Tirol in kleinen Gruppen und deutschsprachige Reisen effet zoloft 50 unvergesslichen Ausflug zu den berühmten Pyramiden von Gizeh sowie der ...
Fmoc-D-Lys(Boc)-OHNα-9-Fluorenylmethoxycarbonyl-Nε-t-butyloxycarbonyl-D-lysine | Fmoc-D-Lys(Boc)2411 1 g | 95.00 EUR 2411 5 g ...
... , also known as lipocortin I, is a protein that is encoded by the ANXA1 gene in humans. Annexin A1 belongs to the ... Annexin A1 has also been shown to be associated with treatment resistance. ARID1A loss activates annexin A1 expression, which ... annexin A1 is promptly mobilized to the cell surface and secreted. Annexin A1 promotes neutrophil detachment and apoptosis, and ... anti-inflammatory effects is to increase the synthesis and function of annexin A1. Annexin A1 both suppresses phospholipase A2 ...
... interleukin 5 and annexin A1). DRESS syndrome: Key elements promoting tissue injury in the DRESS syndrome are: Th2 cells and ...
Annexin A1 List of cutaneous conditions "Hairy cell leukemia: MedlinePlus Medical Encyclopedia". medlineplus.gov. Retrieved ...
Yazid, Samia; Norling, Lucy V.; Flower, Rod J. (2012-08-01). "Anti-inflammatory drugs, eicosanoids and the annexin A1/FPR2 anti ...
2006). "In vitro and in vivo studies on CCR10 regulation by Annexin A1". FEBS Lett. 580 (5): 1431-8. doi:10.1016/j.febslet. ...
"MicroRNA-196a targets annexin A1: a microRNA-mediated mechanism of annexin A1 downregulation in cancers". Oncogene. 27 (52): ...
Lennon NJ, Kho A, Bacskai BJ, Perlmutter SL, Hyman BT, Brown RH (2003). "Dysferlin interacts with annexins A1 and A2 and ... including annexin and MG53. Exactly how dysferlin contributes to membrane resealing is not clear, but biochemical evidence ... "Enhanced Muscular Dystrophy from Loss of Dysferlin Is Accompanied by Impaired Annexin A6 Translocation after Sarcolemmal ...
Nadkarni S, Cooper D, Brancaleone V, Bena S, Perretti M (November 2011). "Activation of the annexin A1 pathway underlies the ...
Biaoxue R, Xiling J, Shuanying Y, Wei Z, Xiguang C, Jinsui W, Min Z (Aug 2012). "Upregulation of Hsp90-beta and annexin A1 ...
Annexin A1 (also termed ANXA1 and lipocortin 1) and its N-terminal peptides (Ac2-26 and Ac9-25). At low concentrations, these ...
However some annexins (Annexin A1, Annexin A2, and Annexin A5) can be secreted from the cytoplasm to outside cellular ... Annexin, type I InterPro: IPR002388 Annexin, type II InterPro: IPR002389 Annexin, type III InterPro: IPR002390 Annexin, type IV ... In addition to annexin A-II, annexin A-XI has also been shown to organize cell membrane properties. Annexin A-XI is believed to ... The 310 amino acid annexin core has four annexin repeats, each composed of 5 alpha-helices. The exception is annexin A-VI that ...
Bohn E, Gerke V, Kresse H, Löffler BM, Kunze H (January 1992). "Annexin II inhibits calcium-dependent phospholipase A1 and ... Annexin A2 also known as annexin II is a protein that in humans is encoded by the ANXA2 gene. Annexin 2 is involved in diverse ... Kwon M, MacLeod TJ, Zhang Y, Waisman DM (January 2005). "S100A10, annexin A2, and annexin a2 heterotetramer as candidate ... Annexin II is a pleiotropic protein meaning that its function is dependent on place and time in the body. The ANXA2 gene, ...
1992). "Annexin II inhibits calcium-dependent phospholipase A1 and lysophospholipase but not triacyl glycerol lipase activities ...
For example, this combination led to demonstrate the calcium-dependent heparin binding of Annexin A1 that is involved in ... "Characterization of annexin A1 glycan binding reveals binding to highly sulfated glycans with preference for highly sulfated ...
The protein Annexin A1 (ANXA1), found in high quantities in the anterior pituitary gland, is located specifically in the ...
Calreticulin, annexin A1, histones, pentraxin-3 and DNA may be released by (and onto the surface of) dying cells to encourage ... annexin A1, histones and pentraxin-3 (PTX3). The most well characterised eat-me signal is the phospholipid phosphatidylserine. ...
Examples of glucocorticoid-responsive genes include those that encode annexin A1, TSC22D3 (also known as GILZ), angiotensin- ...
... and annexin A1−. These cells, similar to the monoclonal cells in Hairy cell leukemia, may have the V600E mutation in the BRAF ...
... annexin A1 (an inhibitor of formation of pro-inflammatory metabolites of polyunsaturated fatty acids), and the gaseous ...
... annexin A1, oxidised LDL and altered glycans. These molecules are recognised by receptors on the cell surface of the macrophage ...
... annexin a1 MeSH D12.776.157.125.050.060 - annexin a2 MeSH D12.776.157.125.050.070 - Annexin A3 MeSH D12.776.157.125.050.080 - ... annexin a4 MeSH D12.776.157.125.050.100 - annexin a5 MeSH D12.776.157.125.050.110 - annexin a6 MeSH D12.776.157.125.050.120 - ... annexin a7 MeSH D12.776.157.125.412.249 - calmodulin MeSH D12.776.157.125.412.311 - calnexin MeSH D12.776.157.125.412.374 - ...
Ac2-26 and Ac9-25 of Annexin A1 (ANXA1 or lipocortin 1), which at high concentrations fully stimulate neutrophil functions but ...
Garbuglia M, Verzini M, Donato R (September 1998). "Annexin VI binds S100A1 and S100B and blocks the ability of S100A1 and ... Protein S100-A1, also known as S100 calcium-binding protein A1 is a protein which in humans is encoded by the S100A1 gene. ... Overview of all the structural information available in the PDB for UniProt: P23297 (Protein S100-A1) at the PDBe-KB. (CS1: ... "Entrez Gene: S100A1 S100 calcium binding protein A1". Morii K, Tanaka R, Takahashi Y, Minoshima S, Fukuyama R, Shimizu N, ...
... a genetic disorder Annexin A1, a human protein Outer membrane phospholipase A1, a bacterial protein receptors α-1-Adrenoceptor ... member A1 Replication protein A1 S100 calcium binding protein A1 Sec61 alpha 1 Serum amyloid A1 Solute carrier family 35 (CMP- ... member A1 UDP glucuronosyltransferase 1 family, polypeptide A1 Urea Transporter A1 a gene found in the maize encoding for the ... member A1 Aldo-keto reductase family 1, member A1 Alpha-1-microglobulin/bikunin precursor Apolipoprotein A1 and ApoA-1 Milano ...
... by competing for phosphatidylserine binding sites with prothrombin and also to inhibit the activity of phospholipase A1. These ... Annexin A5 (or annexin V) is a cellular protein in the annexin group. In flow cytometry, annexin V is commonly used to detect ... The annexin A5 affinity assay typically uses a conjugate of annexin V and a fluorescent or enzymatic label, biotin or other ... Annexin A5 showed upregulation in papillary thyroid carcinoma. Annexin A5 is used as a non-quantitative probe to detect cells ...
... apolipoprotein A-1 (APOA1), peroxiredoxin (PRDX2) and annexin A (ANXA)". Comparative proteomic analyses of 11 cell lines ...
Annexin) CHO1 Cortactin CamKinase II Calponin Chondramide Cortexillin CAP Caltropin CH-ILKBP CPb3 Cap100 Calvasculin Ciboulot ... Myosin light chain kinase MAL Mip-90 Myosin Light Chain A1 MARKS MIM MAYP Mycalolide (a macroglide drug) Mayven Myelin basic ... Afadin AFAP-110 Affixin Aginactin AIP1 Aldolase Angiogenin Anillin Annexins Aplyronine Archvillin Arginine kinase Arp2/3 ...
... a human gene Annexin A3, a human gene ATC code A03 Drugs for functional gastrointestinal disorders, a subgroup of the ... a document given to employees of A-1 and A-2 Visa Holders who are representing a foreign government inside the U.S. A3, the ... a compact car LNER Gresley Classes A1 and A3, a Pacific Locomotive Designed by Sir Nigel Gresley Prussian A 3, a 1910 Prussian ...
A1-4, B1-4, C1-4 and D1-4). The A1-A3 sublineages constitute the European variant, A4 the Asian variant, B1-B4 the African type ... 2012). "The S100A10 subunit of the annexin A2 heterotetramer facilitates L2-mediated human papillomavirus infection". PLOS ONE ... and annexin A2 leading to entry of the virions into basal epithelial cells through clathrin-mediated endocytosis and/or ...
In addition, the tumor cells may also express adenosine A1 and A3 receptors associated with Gαi proteins, promoting both the ... Babiychuk EB, Draeger A (June 2006). "Regulation of ecto-5'-nucleotidase activity via Ca2+-dependent, annexin 2-mediated ...
Family 2.B.8 The Bafilomycin A1 (Bafilomycin) Family 2.B.9 The Cell Penetrating Peptide (CPP) Functional Family 2.B.10 The ... or Na+-translocating bacterial MotAB flagellar motor/ExbBD outer-membrane transport energizer superfamily 1.A.31 Annexin family ...
... cytochromes a1 MeSH D08.244.175.600 - cytochromes a3 MeSH D08.244.187.249 - cytochromes b MeSH D08.244.187.500 - cytochromes b5 ... annexin A3 MeSH D08.811.277.352.640.125 - 3',5'-cyclic-GMP phosphodiesterase MeSH D08.811.277.352.640.150 - 3',5'-cyclic- ...
US-2012004289-A1 chemical patent summary.
Viele dieser Effekte werden durch das glucocorticoid induzierbare Protein Annexin A1 (AnxA1) vermittelt. Das 37 kDa große ... Annexin A1AnxA1RPGNrasch progressive Glomerulonephritisa-GBM GlomerulonephritisNiererapid progressive glomerulonephritisa-GBM ... A broad range of these effects are mediated by the glucocorticoid inducible protein Annexin A1 (AnxA1). The protein is ... Viele dieser Effekte werden durch das glucocorticoid induzierbare Protein Annexin A1 (AnxA1) vermittelt. Das 37 kDa große ...
Proteintechs Mouse Monoclonal Annexin A1 antibody is validated in FC (Intra), IF and shows reactivity with human, mouse ... IF protocol for CL594 Annexin A1 antibody CL594-66344. Download protocol. FC protocol for CL594 Annexin A1 antibody CL594-66344 ... CoraLite®594-conjugated Annexin A1 Monoclonal antibody. Annexin A1 Monoclonal Antibody for FC (Intra), IF. ... Annexin 1, annexin A1, Annexin I, ANX1, ANXA1, Calpactin 2, Calpactin II, Chromobindin 9, Lipocortin I, LPC1, p35 ...
Product Name Annexin A1 (13821P-2). Description Recombinant Human Annexin A1 produced in E. coli is a single, non- glycosylated ... Target Annexin A1. Product Background Annexin A1 is a member of the Ca+2- dependent phospholipid binding proteins that are ... Background Annexin A1 is a member of the Ca+2- dependent phospholipid binding proteins that are localized on the cytosolic face ... Alternative Names Annexin A1. Sequence MAMVSEFLKQ AWFIENEEQE YVQTVKSSKG GPGSAVSPYP TFNPSSDVAA LHKAIMVKGV DEATIIDILT KRNNAQRQQI ...
Annexin 1 (ANXA1) has a well-demonstrated role in early delayed inhibitory feedback of glucocorticoids in the pituitary. ANXA1 ... Evidence for a role of the adenosine 5-triphosphate-binding cassette transporter A1 in the externalization of annexin I from ... "Evidence for a Role of the Adenosine 5-Triphosphate-Binding Cassette Transporter A1 in the Externalization of Annexin I from ... "Evidence for a Role of the Adenosine 5-Triphosphate-Binding Cassette Transporter A1 in the Externalization of Annexin I from ...
Annexin A1 (ANXA1) protein has been implicated in the aggressiveness of several cancer types. Materials and methods: This study ... Overexpression of Annexin A1 Is an Independent Predictor of Longer Overall Survival in Epithelial Ovarian Cancer Maroua Manai 1 ... Annexin A1 (ANXA1) protein has been implicated in the aggressiveness of several cancer types. Materials and methods: This study ... Maroua Manai, Raoudha Doghri, Pascal Finetti, Karima Mrad, Rihab Bouabsa, et al.. Overexpression of Annexin A1 Is an ...
Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.Applications: Suitable for use in EL
Alternative names and symbols: ANNEXIN I; ANX1; LIPOCORTIN I; LPC1; CALPACTIN II. Related products: Rabbit anti Annexin A1 ... Gene name: Annexin A1. Gene symbol: ANXA1. Organism: Homo sapiens. GenBank accession: NM_000700.1. Protein accession: NP_ ...
Annexins A1 and A5 improve organ function and reduce mortality in animal sepsis models, inhibit inflammatory processes, reduce ... Annexins A1 and A5 improve organ function and reduce mortality in animal sepsis models, inhibit inflammatory processes, reduce ... Human annexins play important roles in cell membrane dynamics, as well as mediation of systemic effects. Most notably, annexins ... Human annexins play important roles in cell membrane dynamics, as well as mediation of systemic effects. Most notably, annexins ...
... and annexin A1. In contrast, HCL variant is typically negative for CD25, CD123, and annexin A1, as well as negative for BRAF ...
Annexin-A1 protein and its relationship to cortisol in human saliva. Authors. Fowkes, R.C., Moradi-Bidhendi, N., Branceleone, V ... Annexin A1, an anti-inflammatory regulator, is expressed in human adipocytes. Society for Endocrinology BES 2012. Harrogate, UK ... Annexin A1 N-terminal derived Peptide ac2-26 exerts chemokinetic effects on human neutrophils. Frontiers in Pharmacology. 3 28 ... Annexin-A1 protein and its relationship to cortisol. Fowkes, R.C., Moradi-Bidhendi, N., Branceleone, V., Zariwala, M., Brady, D ...
Annexin A1; Cerebral ischaemia and reperfusion injury; Microglia; Neuroinflammation; Sirtuin 5; Succinylation ... Anexina A1; Isquemia Encefálica; AVC Isquêmico; Sirtuínas; Animais; Camundongos; Anexina A1/genética; Anexina A1/metabolismo; ... SIRT5 interacts with and desuccinylates Annexin A1 (ANXA1) at K166, which in turn decreases its SUMOylation level. Notably, the ... Anexina A1 / Sirtuínas / AVC Isquêmico Tipo de estudo: Prognostic_studies Limite: Animais Idioma: Inglês Revista: J ...
Annexin A1 (McNeil et al., 2006). Accession Number:. P04083. Protein Name:. Annexin A1. ... 8] "Phosphorylation of annexin I by TRPM7 channel-kinase." Dorovkov M.V.et.al. 15485879. [9] "Immunoaffinity profiling of ... 14] "Crystal structure of human annexin I at 2.5-A resolution." Weng X.et.al. 8453382. [15] "NMR solution structure of domain 1 ... of human annexin I shows an autonomous folding unit." Gao J.et.al. 9915835. ...
Taken together, these results point to a neoplastic-transforming property of MWCNT, and the miR221-annexin a1 axis is involved ... Bioinformatic analysis indicated that annexin a1 is a target for miR221 regulation, and it was confirmed by transfecting cells ... Further examination revealed the expression of microRNA 221 (miR221) was gradually decreased, while the annexin a1 expression ... In contrast, overexpression of miR221 or downregulation of ANNEXIN A1 slowed cell migration. ...
A1-2), and Ac-GVGTRHKAL-am (A1-3), from annexin A1, Ac-KMSTVHEIL-am (A2-1) and Ac-SALSGHLET-am (A2-2), from annexin A2, and Ac- ... Human Annexins A1, A2, and A8 as Potential Molecular Targets for Ni(II) Ions. (deposited 03 Nov 2014 09:15) * Human Annexins A1 ... Human annexins A1, A2, and A8, proteins modulating the immune system, contain several such sequences. To test if these proteins ... Human Annexins A1, A2, and A8 as Potential Molecular Targets for Ni(II) Ions ...
Annexin A1 drives macrophage skewing to accelerate muscle regeneration through AMPK activation. McArthur S, Juban G, Gobbetti T ...
Modulating hepatic macrophages with annexin A1 in non-alcoholic steatohepatitis Federico Francesco De Ponti (UGent) and ...
Annexin A1 Polyclonal Antibody. Advanced Verification 39 References Advanced Verification 39 References ...
Annexin A1- but CD10+ hairy cell leukemia.. Blood. 2022;139:1924.. PubMed *KATER AP, Melenhorst JJ Do CARs finally hit the CLL ...
Annexin A1 Arginase I ASC Bcl-2 beta Catenin 1 C3/C3b/iC3b C3/C3b/iC3b/C3d C5L2 Cadherin 11 CCL2 CCL5 CD3/CD4/CD45 CD11a CD11b ...
Annexin A1. Molecular Weight. 38713.855 Da. References. *Serres M, Viac J, Comera C, Schmitt D: Expression of annexin I in ...
Pleiotrophin (PTN) is up-regulated in human endometrial epithelial cells [60], and annexin A1 (ANXA1) is up-regulated in a ... Castro-Caldas M, Duarte CB, Carvalho AR, Lopes MC: 17beta-estradiol promotes the synthesis and the secretion of annexin I in ...
Loss of Annexin A1 Expression in Breast Cancer Progression. Appl Immunohistochem Mol Morphol 16(6):530-4, 2008. ... Cell-specific dysregulation of annexin I expression in breast cancer. Mod Pathol 16(1):21A, 2003. ...
Mimetic peptide AC2-26 of annexin A1 as a potential therapeutic agent to treat COPD. International Immunopharmacology 2018;63: ...
Annexin A1 Apolipoprotein D Apolipoprotein M Apoptosis Inhibitor of Macrophage Arginase I beta Actin Beta-Microseminoprotein ... Molecule: Annexin A1 About molecule Product. Size. Price. Annexin A1 Human, Rabbit Polyclonal Antibody Type: Polyclonal ...
Therapeutic Potential of Annexin A1 in Ischemia Reperfusion Injury. Ansari, J; Kaur, G; Gavins, F. ...
Leoni G, Neumann PA, Kamaly N, Quiros M, Nishio H, Jones HR, Sumagin R, Hilgarth RS, Alam A, Fredman G, et al: Annexin A1- ... Guido BC, Zanatelli M, Tavares-De-Lima W, Oliani SM and Damazo AS: Annexin-A1 peptide down-regulates the leukocyte recruitment ... Yang YH, Morand E and Leech M: Annexin A1: Potential for glucocorticoid sparing in RA. Nat Rev Rheumatol. 10:595-603. 2013. ... Perretti M and DAcquisto F: Annexin A1 and glucocorticoids as effectors of the resolution of inflammation. Nat Rev Immunol. 1: ...
Effect of annexin A1 and its mimetic peptides in models of inflammatory response in vitro (2D and 3D) and acute toxicity in ... Role of Annexin A1 in NLRP3 Inflammasome Activation in Murine Neutrophils. CELLS, v. 10, n. 1, JAN 2021. (20/03565-2, 19/19949- ... Effect of annexin A1 and its mimetic peptides in models of inflammatory response in vitro (2D and 3D) and acute toxicity in ... he role of annexin A1 in Candida albicans and Candida auris infections in murine neutrophil. Microbial Pathogenesis, v. 150, ...
... called Annexin A1, which stimulates cancer growth. They then confirmed both in vitro and later in mice that disabling Annexin ... Patients with increased Annexin A1 expression had a worse prognosis, regardless of the cancer grade and stage, age, or sex.. ... The researchers are currently looking for ways to develop Annexin A1 as a biomarker for more aggressive cancers and as a ... The researchers also discovered that F. nucleatum increases production of Annexin A1, attracting more of the bacteria. "We ...
  • Viele dieser Effekte werden durch das glucocorticoid induzierbare Protein Annexin A1 (AnxA1) vermittelt. (tu-berlin.de)
  • Annexin 1 (ANXA1) has a well-demonstrated role in early delayed inhibitory feedback of glucocorticoids in the pituitary. (ox.ac.uk)
  • Annexin A1 (ANXA1) protein has been implicated in the aggressiveness of several cancer types. (archives-ouvertes.fr)
  • Annexin-A1 (AnxA1) is an anti-inflammatory protein whose expression is modulated by glucocorticoids. (westminster.ac.uk)
  • SIRT5 interacts with and desuccinylates Annexin A1 (ANXA1) at K166, which in turn decreases its SUMOylation level. (bvsalud.org)
  • Annexin A1 (AnxA1) is a 37 kDa protein capable of regulating various cellular and molecular stages of the inflammatory response and is involved in endogenous mechanisms that are activated to obtain an adequate resolution. (fapesp.br)
  • Annexin A5 is a 36-kDa endogenous protein and part of a 12-member family of ubiquitously and constitutively expressed proteins, and possesses anticoagulant, anti-inflammatory and anti-apoptotic properties. (frontiersin.org)
  • Further examination revealed the expression of microRNA 221 (miR221) was gradually decreased, while the annexin a1 expression was increased at both the mRNA and protein level during the exposure. (biomedcentral.com)
  • In the current study, the researchers found in cell cultures that noncancerous colon cells lack a protein, called Annexin A1, which stimulates cancer growth. (nairaland.com)
  • For this purpose, a pair of antibodies raised against the ANX-A1 N-terminus (ie, able to recognize intact ANX-A1) or the whole protein (ie, able to interact with all ANX-A1 isoforms) was used by immunofluorescence and immunocytochemistry analyses. (ox.ac.uk)
  • MDX-124 has a unique target - a protein called annexin-A1, which has a key function in the immune system. (lifesciencesscotland.com)
  • Protein of the annexin family exhibiting lipid interaction and steroid-inducibility. (bvsalud.org)
  • Annexin A2 (calpactin I, chromobindin 8, p36, Lipocortin II, PAP-IV, or Protein I) is a cytoskeletal calcium-dependent phospholipid binding protein, which has been shown to be a mediator of cortocosteroid activity, a substrate for serine/threonine kinases and growth regulated tyrosine kinases, and may play a role in secretion. (ecmbio.com)
  • This antibody detects a 36 kDa* protein corresponding to the molecular mass of Annexin A2 on SDS-PAGE immunoblots of rat PC12 cells. (ecmbio.com)
  • In numerous studies, treatment with recombinant human annexins and annexin analogue peptides have consistently found positive outcomes in animal models of sepsis, myocardial infarction, and ischemia reperfusion injury. (frontiersin.org)
  • Several studies have shown that Ac2‑26, the active N ‑terminal peptide of Annexin A1, could modulate anti‑inflammatory processes and protect the organs from ischemia‑reperfusion injury (IRI). (spandidos-publications.com)
  • These discoveries led to the development of analogous peptides which mimic their physiological function, and investigation into the potential of using the annexins and their analogous peptides as therapeutic agents in conditions where inflammation and coagulation play a large role in the pathophysiology. (frontiersin.org)
  • The Annexin family of calcium-binding proteins is composed of al least thirteen mammalian genes (Annexin A1-13). (ecmbio.com)
  • Given the involvement of phospholipase A2 in mediating inflammation, prostaglandins, and leukotrienes, Annexin A1 might have potential anti-inflammatory activity. (qedbio.com)
  • Most notably, annexins are highly involved in anti-inflammatory processes, adaptive immunity, modulation of coagulation and fibrinolysis, as well as protective shielding of cells from phagocytosis. (frontiersin.org)
  • Product Background Annexin A1 is a member of the Ca+2- dependent phospholipid binding proteins that are localized on the cytosolic face of the plasma membrane. (qedbio.com)
  • Detailed analyses demonstrated miR221 was involved in the regulation of cell migration, e.g., downregulation of miR221 or overexpression of ANNEXIN A1, contributed to the increased cell migration. (biomedcentral.com)
  • In contrast, overexpression of miR221 or downregulation of ANNEXIN A1 slowed cell migration. (biomedcentral.com)
  • Annexin A6 reverses transformation of A431 cells after overexpression, and this effect may involve annexin A6 targeting of p120 RasGAP to the plasma membrane to inactivate Ras. (ecmbio.com)
  • Characterization of annexin A1 glycan binding reveals binding to highly sulfated glycans with preference for highly sulfated heparan sulfate and heparin. (mpg.de)
  • The blot was probed with mouse monoclonal anti-Annexin A2 antibody at 1:250 (lane 1) or 1:1000 (lane 2). (ecmbio.com)
  • The cells were fixed in paraformaldehyde and permeabilized using NP-40, then labeled with mouse monoclonal Annexin A2 (C-terminal region). (ecmbio.com)
  • The effects of annexin A5 on inflammation and platelet activation may be particularly beneficial in disease caused by SARS-CoV-2 infection. (frontiersin.org)
  • Annexin 1 (ANX-A1) exerts antimigratory actions in several models of acute and chronic inflammation. (ox.ac.uk)
  • Description Recombinant Human Annexin A1 produced in E. coli is a single, non- glycosylated polypeptide chain containing 346 amino acids with a molecular weight of 38.7kDa. (qedbio.com)
  • Safety and efficacy of recombinant human annexin A5 are currently being studied in clinical trials in sepsis and severe COVID-19 patients. (frontiersin.org)
  • Clone (M298) was generated from a recombinant sequence that included amino acids in the C-terminal region of rat Annexin A2. (ecmbio.com)
  • Evidence for a role of the adenosine 5'-triphosphate-binding cassette transporter A1 in the externalization of annexin I from pituitary folliculo-stellate cells. (ox.ac.uk)
  • In conclusion, biochemical pathways leading to the externalization, proteolysis, and synthesis of ANX-A1 are activated during the process of neutrophil extravasation. (ox.ac.uk)
  • Altogether, our results indicate that human annexins A1, A2, and A8 are potential molecular targets for nickel toxicity and help design appropriate cellular studies. (waw.pl)
  • CL594-66344 targets Annexin A1 in IF, FC (Intra) applications and shows reactivity with human, mouse samples. (ptglab.com)
  • 1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Annexin A1 (CL594-66344, Clone:1E1B7) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). (ptglab.com)
  • Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite®594 Annexin A1 antibody (CL594-66344, Clone: 1E1B7 ) at dilution of 1:200. (ptglab.com)
  • This is related to its ability to mimic the effect of endogenous ANX-A1 that is externalized on neutrophil adhesion to the postcapillary endothelium. (ox.ac.uk)
  • The majority ( approximately 50%) of ANX-A1 on the plasma membrane of intravascular neutrophils was intact. (ox.ac.uk)
  • Human annexins play important roles in cell membrane dynamics, as well as mediation of systemic effects. (frontiersin.org)
  • 14] "Crystal structure of human annexin I at 2.5-A resolution. (tcdb.org)
  • 15] "NMR solution structure of domain 1 of human annexin I shows an autonomous folding unit. (tcdb.org)
  • In contrast, HCL variant is typically negative for CD25, CD123, and annexin A1, as well as negative for BRAF V600E. (medscape.com)
  • The researchers also discovered that F. nucleatum increases production of Annexin A1, attracting more of the bacteria. (nairaland.com)
  • The researchers are currently looking for ways to develop Annexin A1 as a biomarker for more aggressive cancers and as a potential target for developing new treatments for colon and other types of cancer. (nairaland.com)
  • Annexins are molecules that show efficacy in preclinical models of sepsis but have not been investigated as a potential therapy in patients with sepsis. (frontiersin.org)
  • Patients with increased Annexin A1 expression had a worse prognosis, regardless of the cancer grade and stage, age, or sex. (nairaland.com)
  • Bioinformatic analysis indicated that annexin a1 is a target for miR221 regulation, and it was confirmed by transfecting cells with miR221 mimics, which resulted in the downregulation of annexin a1 . (biomedcentral.com)
  • Neutrophil interaction with inflamed postcapillary venule endothelium alters annexin 1 expression. (ox.ac.uk)
  • Taken together, these results point to a neoplastic-transforming property of MWCNT, and the miR221- annexin a1 axis is involved in the regulation of cell migration in the transformed cells. (biomedcentral.com)
  • They then confirmed both in vitro and later in mice that disabling Annexin A1 prevented F. nucleatumfrom binding to the cancer cells, slowing their growth. (nairaland.com)
  • In the cytoplasm of these cells, ANX-A1 was predominantly associated with large vacuoles, possibly endosomes. (ox.ac.uk)
  • In situ hybridization confirmed de novo synthesis of ANX-A1 in the extravasated cells. (ox.ac.uk)
  • CAF-secreted annexin A1 induces prostate cancer cells to gain stem cell-like features. (sc-ctsi.org)
  • Immunocytochemical labeling of Annexin A2 in NGF-differentiated rat PC12 cells. (ecmbio.com)
  • The antibody has been shown to label Annexin A2 in immunocytochemical analysis of PC12 cells and immunohistochemical analysis of mouse diaphragm. (ecmbio.com)
  • Annexins A1 and A5 improve organ function and reduce mortality in animal sepsis models, inhibit inflammatory processes, reduce inflammatory mediator release, and protect against ischemic injury. (frontiersin.org)
  • The Annexin family has been implicated as regulators of such diverse processes as ion-flux, endocytosis and exocytosis, and cellular adhesion. (ecmbio.com)
  • In the present study we monitored ANX-A1 expression and localization in intravascular and emigrated neutrophils, using a classical model of rat peritonitis. (ox.ac.uk)
  • A role for stroma-derived annexin A1 as mediator in the control of genetic susceptibility to T-cell lymphoblastic malignancies through prostaglandin E2 secretion. (pasteur.fr)
  • Although annexin-A1 is known to play a significant role in the development of numerous cancers and autoimmune diseases, this is the first time that it has been investigated therapeutically. (lifesciencesscotland.com)
  • The mechanisms of action and demonstrated efficacy of annexins in animal models support development of annexins and their analogues for the treatment of sepsis. (frontiersin.org)