Annexin A2: A member of the annexin family that is a substrate for a tyrosine kinase, ONCOGENE PROTEIN PP60(V-SRC). Annexin A2 occurs as a 36-KDa monomer and in a 90-KDa complex containing two subunits of annexin A2 and two subunits of S100 FAMILY PROTEIN P11. The monomeric form of annexin A2 was formerly referred to as calpactin I heavy chain.Annexin A1: Protein of the annexin family exhibiting lipid interaction and steroid-inducibility.Annexin A5: A protein of the annexin family isolated from human PLACENTA and other tissues. It inhibits cytosolic PHOSPHOLIPASE A2, and displays anticoagulant activity.Annexin A6: Protein of the annexin family with a probable role in exocytotic and endocytotic membrane events.Annexin A4: Protein of the annexin family originally isolated from the electric organ of the electric ray Torpedo marmorata. It has been found in a wide range of mammalian tissue where it is localized to the apical membrane of polarized EPITHELIAL CELLS.Annexin A7: An annexin family member that plays a role in MEMBRANE FUSION and signaling via VOLTAGE-DEPENDENT CALCIUM CHANNELS.Annexin A3: A protein of the annexin family that catalyzes the conversion of 1-D-inositol 1,2-cyclic phosphate and water to 1-D-myo-inositol 1-phosphate.Annexins: Family of calcium- and phospholipid-binding proteins which are structurally related and exhibit immunological cross-reactivity. Each member contains four homologous 70-kDa repeats. The annexins are differentially distributed in vertebrate tissues (and lower eukaryotes) and appear to be involved in MEMBRANE FUSION and SIGNAL TRANSDUCTION.S100 Proteins: A family of highly acidic calcium-binding proteins found in large concentration in the brain and believed to be glial in origin. They are also found in other organs in the body. They have in common the EF-hand motif (EF HAND MOTIFS) found on a number of calcium binding proteins. The name of this family derives from the property of being soluble in a 100% saturated ammonium sulfate solution.Phosphatidylserines: Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a serine moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and serine and 2 moles of fatty acids.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.Propidium: Quaternary ammonium analog of ethidium; an intercalating dye with a specific affinity to certain forms of DNA and, used as diiodide, to separate them in density gradients; also forms fluorescent complexes with cholinesterase which it inhibits.Calcium: A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.Phospholipids: Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.Calcium-Binding Proteins: Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Cell Line, Tumor: A cell line derived from cultured tumor cells.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Flow Cytometry: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Cell-Derived Microparticles: Extracellular vesicles generated by the shedding of CELL MEMBRANE blebs.Receptors, Formyl Peptide: A family of G-protein-coupled receptors that was originally identified by its ability to bind N-formyl peptides such as N-FORMYLMETHIONINE LEUCYL-PHENYLALANINE. Since N-formyl peptides are found in MITOCHONDRIA and BACTERIA, this class of receptors is believed to play a role in mediating cellular responses to cellular damage and bacterial invasion. However, non-formylated peptide ligands have also been found for this receptor class.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Caspase 3: A short pro-domain caspase that plays an effector role in APOPTOSIS. It is activated by INITIATOR CASPASES such as CASPASE 9. Isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.Organotechnetium Compounds: Organic compounds that contain technetium as an integral part of the molecule. These compounds are often used as radionuclide imaging agents.Cell Survival: The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Caspases: A family of intracellular CYSTEINE ENDOPEPTIDASES that play a role in regulating INFLAMMATION and APOPTOSIS. They specifically cleave peptides at a CYSTEINE amino acid that follows an ASPARTIC ACID residue. Caspases are activated by proteolytic cleavage of a precursor form to yield large and small subunits that form the enzyme. Since the cleavage site within precursors matches the specificity of caspases, sequential activation of precursors by activated caspases can occur.Fluorescein-5-isothiocyanate: Fluorescent probe capable of being conjugated to tissue and proteins. It is used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.In Situ Nick-End Labeling: An in situ method for detecting areas of DNA which are nicked during APOPTOSIS. Terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 prime OH ends of either single- or double-stranded DNA. The terminal deoxynucleotidyl transferase nick end labeling, or TUNEL, assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA FRAGMENTATION.Fibrinolysin: A product of the lysis of plasminogen (profibrinolysin) by PLASMINOGEN activators. It is composed of two polypeptide chains, light (B) and heavy (A), with a molecular weight of 75,000. It is the major proteolytic enzyme involved in blood clot retraction or the lysis of fibrin and quickly inactivated by antiplasmins.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.

Down-regulation of microglial cyclo-oxygenase-2 and inducible nitric oxide synthase expression by lipocortin 1. (1/385)

1. Activated microglial cells are believed to play an active role in most brain pathologies, during which they can contribute to host defence and repair but also to the establishment of tissue damage. These actions are largely mediated by microglial secretory products, among which are prostaglandins (PGs) and nitric oxide (NO). 2. The anti-inflammatory protein, lipocortin 1 (LC1) was reported to have neuroprotective action and to be induced by glucocorticoids in several brain structures, with a preferential expression in microglia. In this paper we tested whether the neuroprotective effect of LC1 could be explained by an inhibitory effect on microglial activation. 3. We have previously shown that bacterial endotoxin (LPS) strongly stimulates PGE2 and NO production in rat primary microglial cultures, by inducing the expression of the key enzymes cyclo-oxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), respectively. 4. Dexamethasone (DEX, 1-100 nM) and LC1-derived N-terminus peptide (peptide Ac2-26, 1-100 microg ml(-1)) dose-dependently inhibited the production of both PGE2 and NO from LPS-stimulated microglia. The inhibitory effects of DEX on NO and of the peptide on NO and PGE2 synthesis were partially abrogated by a specific antiserum, raised against the N-terminus of human LC1. The peptide Ac2-26 did not affect arachidonic acid release from control and LPS-stimulated microglial cultures. 5. Western blot experiments showed that the LPS-induced expression of COX-2 and iNOS was effectively down-regulated by DEX (100 nM) and peptide Ac2-26 (100 microg ml(-1)). 6. In conclusion, our findings support the hypothesis that LC1 may foster neuroprotection by limiting microglial activation, through autocrine and paracrine mechanisms.  (+info)

Modulation of cellular annexin I in human leukocytes infiltrating DTH skin reactions. (2/385)

Based on our previous studies showing endogenous annexin I being depleted from migrated neutrophils (PMN) in vitro, we have tested whether the levels of this glucocorticoid-regulated protein in PMN and mononuclear cells (PBMC) were modified after adhesion to endothelial monolayers in vitro and extravasation into skin blisters in vivo. In vitro, annexin I levels were depleted more significantly (-70%) in post-adherent PMNs than in monocytes (-25%) and lymphocytes (-50%, only in the positive fraction). In vivo, a significant time-dependent increase (approximately threefold, P < 0.05) in cell-associated annexin I was measured in PBMCs recovered from the blisters, whereas no significant changes were detected in extravasated PMNs. This was associated with annexin I release in the blister fluids (approximately 35 ng/mL), whereas no detectable protein was found in matched-paired plasmas. In conclusion, we report for the first time an activation of the annexin I pathway during an ongoing experimental inflammatory response in humans, which is differently regulated between PMNs and PBMCs.  (+info)

Inhibitory effect of annexin I on synovial inflammation in rat adjuvant arthritis. (3/385)

OBJECTIVE: Annexin I is an endogenous antiinflammatory mediator, expressed in rheumatoid arthritis (RA) synovium, the contribution of which to autoregulation of the synovial inflammatory response has not been examined in models of RA. We investigated the antiinflammatory role of annexin I in rat adjuvant arthritis. METHODS: Rats with adjuvant-induced arthritis (AIA) were treated with a specific anti-annexin I monoclonal antibody (mAb), isotype control IgG, and/or dexamethasone. Clinical outcomes and synovial synthesis of tumor necrosis factor alpha (TNFalpha), prostaglandin E2 (PGE2), and nitric oxide were examined, and annexin I expression was assessed by flow cytometry and reverse transcription-polymerase chain reaction. RESULTS: Anti-annexin I mAb reversed the effects of dexamethasone on the clinical features of AIA and exacerbated AIA in the absence of exogenous glucocorticoid. Clinical exacerbation of AIA by anti-annexin I mAb was accompanied by significantly increased synovial TNFalpha and PGE2, suggesting that annexin I tonically inhibits the production of these mediators. Anti-annexin I mAb treatment was associated with significantly reduced leukocyte intracellular annexin I, despite increased annexin I messenger RNA expression, consistent with a depletion effect of extracellular mAb via the cell surface. CONCLUSION: Annexin I is a key endogenous inhibitory mediator of arthritis via mechanisms that include inhibition of cytokine and effector molecule production. Moreover, a synthesis-independent depletion of intracellular annexin I by extracellular antibody supports the hypothesis that externalization of annexin I is involved in its mode of action.  (+info)

Annexin I modulates cell functions by controlling intracellular calcium release. (4/385)

Annexin I is an intracellular protein in search of a function. Ex vivo it has calcium- and phospholipid-binding properties. To evaluate its role in vivo, MCF-7 cells were stably transfected with annexin I in sense or antisense orientations. In cells overexpressing annexin I, calcium release was abrogated on stimulation of purinergic or bradykinin receptors, whereas non-transfected cells or cells with down-regulated annexin I released calcium within seconds. Basal calcium and calcium stores were not affected. The impaired calcium release was paralleled by a down-regulation of the activities of phospholipase C, group II phospholipase A2, and E-cadherin with altered adhesion and enhanced tumor growth on soft agar. Significantly smaller tumors, with the histologically most differentiated cells, were observed in nude mice inoculated with cells transfected with the antisense rather than with the sense plasmid. These observations indicate that annexin I modulates cell functions by controlling intracellular calcium release. Frey, B. M., Reber, B. F. X., Vishwanath, B. S., Escher, G., Frey, F. J. Annexin I modulates cell functions by controlling intracellular calcium release.  (+info)

The inhibitory effect of dexamethasone on lymphocyte adhesion molecule expression and intercellular aggregation is not mediated by lipocortin 1. (5/385)

Glucocorticoids exert their anti-inflammatory activity through multiple pathways which include the inhibition of cell adhesion events. The glucocorticoid-induced protein lipocortin 1 (LC1) has reported anti-inflammatory properties and has been proposed as a putative mediator of the anti-inflammatory effects of glucocorticoids. The role of LC1 in mediating the glucocorticoid inhibition of lymphocyte adhesion and cell adhesion molecule (CAM) expression was investigated in vitro using a microaggregation assay, flow cytometry and confocal microscopy. Lymphocytes stimulated for 96 h with plastic-bound OKT3 antibody showed significant increases in LFA-1 and CD2 expression. Dexamethasone (DEX; 10(-6) M) inhibited this increase but the neutralizing anti-LC1 MoAb 1A (5 microg/ml) failed to reverse the DEX effect; neither was purified human LC1 (50 x 10(-9) M) able to inhibit CAM expression. The biological activity of the LC1 was confirmed by its ability to suppress monocyte phagocytosis and respiratory burst in response to bovine serum albumin (BSA)-anti-BSA complexes. OKT3 stimulation of cultured mononuclear cells resulted in intercellular aggregation, scored microscopically using a visual index. This aggregation was completely reversed by 10-6 M DEX but unaffected by LC1 (50 x 10(-9) M). Significant intracellular expression of lymphocyte LC1 was observed using the anti-LC1 MoAb 1B in saponin-permeabilized cells. Distribution of LC1 had a diffuse, cytoplasmic pattern. LC1 expression was reduced following 3 h treatment with 10(-6) M DEX. These findings indicate that the DEX effects on lymphocyte adhesion and CAM expression are not mediated by LC1. Thus the reported in vivo effects of LC1 on leucocyte adhesion and transmigration probably occur through functional/conformation changes of surface CAM, rather than by alteration in expression.  (+info)

The annexin protein lipocortin 1 regulates the MAPK/ERK pathway. (6/385)

Lipocortin 1 (annexin 1) is a calcium- and phospholipid-binding protein that modulates anti-inflammatory responses including those induced by lipopolysaccharide. To investigate the precise role of lipocortin 1 in regulating the lipopolysaccharide-induced signal transduction pathways, we generated stable RAW 264.7 macrophage cell lines expressing decreased and increased lipocortin 1 protein. Several RAW 264.7 clones with increased lipocortin 1 protein levels showed constitutive activation of the mitogen-activated protein kinase extracellular signal-regulated kinase, which was down-regulated following lipopolysaccharide treatment. Conversely, clones with decreased lipocortin 1 protein expression showed prolonged extracellular signal-regulated kinase activity, following lipopolysaccharide activation. Lipocortin 1 specifically regulates the components of the extracellular signal-regulated kinase pathway, since changes in lipocortin 1 protein expression had no affect on the related mitogen-activated protein kinases p38 and c-Jun N-terminal kinase. Lipocortin 1 modulated upstream components of the extracellular signal-regulated kinase pathway and associated with the adaptor protein growth factor binding protein. The downstream consequences of altered extracellular signal-regulated kinase activity were independent of the proinflammatory transcription factor nuclear factor kappa B. These data indicate that lipocortin 1 specifically regulates proximal signaling components of the extracellular signal-regulated kinase signal transduction pathway, resulting in the modulation of biochemical functions in RAW macrophages.  (+info)

Structural basis of the Ca(2+)-dependent association between S100C (S100A11) and its target, the N-terminal part of annexin I. (7/385)

BACKGROUND: S100C (S100A11) is a member of the S100 calcium-binding protein family, the function of which is not yet entirely clear, but may include cytoskeleton assembly and dynamics. S100 proteins consist of two EF-hand calcium-binding motifs, connected by a flexible loop. Like several other members of the family, S100C forms a homodimer. A number of S100 proteins form complexes with annexins, another family of calcium-binding proteins that also bind to phospholipids. Structural studies have been undertaken to understand the basis of these interactions. RESULTS: We have solved the crystal structure of a complex of calcium-loaded S100C with a synthetic peptide that corresponds to the first 14 residues of the annexin I N terminus at 2.3 A resolution. We find a stoichiometry of one peptide per S100C monomer, the entire complex structure consisting of two peptides per S100C dimer. Each peptide, however, interacts with both monomers of the S100C dimer. The two S100C molecules of the dimer are linked by a disulphide bridge. The structure is surprisingly close to that of the p11-annexin II N-terminal peptide complex solved previously. We have performed competition experiments to try to understand the specificity of the S100-annexin interaction. CONCLUSIONS: By solving the structure of a second annexin N terminus-S100 protein complex, we confirmed a novel mode of interaction of S100 proteins with their target peptides; there is a one-to-one stoichiometry, where the dimeric structure of the S100 protein is, nevertheless, essential for complex formation. Our structure can provide a model for a Ca(2+)-regulated annexin I-S100C heterotetramer, possibly involved in crosslinking membrane surfaces or organising membranes during certain fusion events.  (+info)

Detection of annexins I and IV in bronchoalveolar lavage fluids from calves inoculated with bovine herpes virus-1. (8/385)

Annexins are phospholipid-binding proteins and are abundant in the lung. Annexins I and IV, but not II and VI, have been detected in bronchoalveolar lavage (BAL) fluids from calves inoculated with Pasteurella haemolytica, the pathogen for calf pneumonia. In this study, BAL fluids from calves with experimental pneumonia induced by inoculation to right lung lobes of bovine herpes virus-1 (BHV-1), the major viral pathogen for pneumonia, were examined for detection of annexins I and IV. Of 6 calves inoculated with BHV-1, annexins I and IV were coincidentally detected in BAL fluids from right lung lobes of 4 calves, but not in BAL fluids from left lung lobes of 6 inoculated calves or those from left and right lung lobes of 3 control calves. Annexin II and VI were not found in any BAL fluids examined. These results, together with previous findings on calves inoculated with Pasteurella haemolytica, suggest that the release of annexins I and IV onto the alveolar surface is an essential event occurring in response to pulmonary infections of BHIV-1 and Pasteurella haemolytica.  (+info)

*Annexin A1

... , also known as lipocortin I, is a protein that is encoded by the ANXA1 gene in humans. Annexin A1 belongs to the ... Annexin A1 has also been shown to be associated with treatment resistance. ARID1A loss activates annexin A1 expression, which ... annexin A1 is promptly mobilized to the cell surface and secreted. Annexin A1 promotes neutrophil detachment and apoptosis, and ... anti-inflammatory effects is to increase the synthesis and function of annexin A1. Annexin A1 both suppresses phospholipase A2 ...

*Formyl peptide receptor 1

Annexin A1 (also termed ANXA1 and lipocortin 1) and its N-terminal peptides (Ac2-26 and Ac9-25). At low concentrations, these ...

*CCR10

2006). "In vitro and in vivo studies on CCR10 regulation by Annexin A1". FEBS Lett. 580 (5): 1431-8. doi:10.1016/j.febslet. ...

*Dysferlin

Lennon NJ, Kho A, Bacskai BJ, Perlmutter SL, Hyman BT, Brown RH (2003). "Dysferlin interacts with annexins A1 and A2 and ... including annexin and MG53. Exactly how dysferlin contributes to membrane resealing is not clear, but biochemical evidence ...

*Mir-584 microRNA precursor family

"MicroRNA-196a targets annexin A1: A microRNA-mediated mechanism of annexin A1 downregulation in cancers". Oncogene. 27 (52): ...

*Estrogen

Nadkarni S, Cooper D, Brancaleone V, Bena S, Perretti M (November 2011). "Activation of the annexin A1 pathway underlies the ...

*HSP90AB1

Biaoxue R, Xiling J, Shuanying Y, Wei Z, Xiguang C, Jinsui W, Min Z (Aug 2012). "Upregulation of Hsp90-beta and annexin A1 ...

*Glycan array

For example, this combination led to demonstrate the calcium-dependent heparin binding of Annexin A1 that is involved in ... "Characterization of annexin A1 glycan binding reveals binding to highly sulfated glycans with preference for highly sulfated ...

*Folliculostellate cell

The protein Annexin A1 (ANXA1), found in high quantities in the anterior pituitary gland, is located specifically in the ...

*Annexin

However some annexins (Annexin A1, Annexin A2, and Annexin A5) have also been found outside the cellular environment, for ... Annexin, type I InterPro: IPR002388 Annexin, type II InterPro: IPR002389 Annexin, type III InterPro: IPR002390 Annexin, type IV ... In addition to annexin A-II, annexin A-XI has also been shown to organize cell membrane properties. Annexin A-XI is believed to ... The 310 amino acid annexin core has four annexin repeats, each composed of 5 alpha-helices. The exception is annexin A-VI that ...

*Selective glucocorticoid receptor modulator

Examples of glucocorticoid-responsive genes include those that encode annexin A1, TSC22D3 (also known as GILZ), angiotensin- ...

*Adult T-cell leukemia/lymphoma

Chen, Z; Yoshihara E (2010). "Differential roles of Annexin A1 (ANXA1/lipocortin-1/lipomodulin) and thioredoxin binding protein ...

*Specialized pro-resolving mediators

... annexin A1 (an inhibitor of formation of pro-inflammatory metabolites of polyunsaturated fatty acids), and the gaseous ...

*Phagocytosis

... annexin A1, oxidised LDL and altered glycans. These molecules are recognised by receptors on the cell surface of the macrophage ...

*List of MeSH codes (D12.776.157)

... annexin a1 MeSH D12.776.157.125.050.060 -- annexin a2 MeSH D12.776.157.125.050.070 -- Annexin A3 MeSH D12.776.157.125.050.080 ... annexin a4 MeSH D12.776.157.125.050.100 -- annexin a5 MeSH D12.776.157.125.050.110 -- annexin a6 MeSH D12.776.157.125.050.120 ... annexin a7 MeSH D12.776.157.125.412.249 -- calmodulin MeSH D12.776.157.125.412.311 -- calnexin MeSH D12.776.157.125.412.374 -- ...

*Formyl peptide receptor 2

Ac2-26 and Ac9-25 of Annexin A1 (ANXA1 or lipocortin 1), which at high concentrations fully stimulate neutrophil functions but ...

*LYPLA1

1992). "Annexin II inhibits calcium-dependent phospholipase A1 and lysophospholipase but not triacyl glycerol lipase activities ...

*Annexin A2

Bohn E, Gerke V, Kresse H, Löffler BM, Kunze H (Jan 1992). "Annexin II inhibits calcium-dependent phospholipase A1 and ... Annexin A2 also known as annexin II is a protein that in humans is encoded by the ANXA2 gene. Annexin 2 is involved in diverse ... Kwon M, MacLeod TJ, Zhang Y, Waisman DM (Jan 2005). "S100A10, annexin A2, and annexin a2 heterotetramer as candidate ... "Entrez Gene: ANXA2 annexin A2". Kling T, Ferrarese R, Ó hAilín D, Johansson P, Heiland DH, Dai F, Vasilikos I, Weyerbrock A, ...

*List of A1 genes, proteins or receptors

... a genetic disorder Annexin A1, a human protein Outer membrane phospholipase A1, a bacterial protein receptors α-1-Adrenoceptor ... member A1 Replication protein A1 S100 calcium binding protein A1 Sec61 alpha 1 Serum amyloid A1 Solute carrier family 35 (CMP- ... member A1 UDP glucuronosyltransferase 1 family, polypeptide A1 Urea Transporter A1 a gene found in the maize encoding for the ... member A1 Aldo-keto reductase family 1, member A1 Alpha-1-microglobulin/bikunin precursor Apolipoprotein A1 and ApoA-1 Milano ...

*Annexin A5

... by competing for phosphatidylserine binding sites with prothrombin and also to inhibit the activity of phospholipase A1. These ... Annexin A5 (or annexin V) is a cellular protein in the annexin group. In flow cytometry, annexin V is commonly used to detect ... The annexin A5 affinity assay typically uses a conjugate of annexin V and a fluorescent or enzymatic label, biotin or other ... Annexin A5 showed upregulation in papillary thyroid carcinoma. Annexin A5 is used as a non-quantitative probe to detect cells ...

*Actin-binding protein

Annexin) CHO1 Cortactin CamKinase II Calponin Chondramide Cortexillin CAP Caltropin CH-ILKBP CPb3 Cap100 Calvasculin Ciboulot ... Myosin light chain kinase MAL Mip-90 Myosin Light Chain A1 MARKS MIM MAYP Mycalolide (a macroglide drug) Mayven Myelin basic ... Afadin AFAP-110 Affixin Aginactin AIP1 Aldolase Angiogenin Anilin Annexins Aplyronine Archvillin Arginine kinase Arp2/3 complex ...

*A3

... a human gene Annexin A3, a human gene ATC code A03 Drugs for functional gastrointestinal disorders, a subgroup of the ... a document given to employees of A-1 and A-2 Visa Holders who are representing a foreign government inside the U.S. A3, the ... an 1851 German steam locomotive model LNER Gresley Classes A1 and A3, a Pacific Locomotive Designed by Sir Nigel Gresley PRR A3 ...

*List of MeSH codes (D08)

... annexin A3 MeSH D08.811.277.352.640.125 --- 3',5'-cyclic-GMP phosphodiesterase MeSH D08.811.277.352.640.150 --- 3',5'-cyclic- ... cytochromes a1 MeSH D08.244.175.600 --- cytochromes a3 MeSH D08.244.187.249 --- cytochromes b MeSH D08.244.187.500 --- ...

*Transporter Classification Database

Family 2.B.8 The Bafilomycin A1 (Bafilomycin) Family 2.B.9 The Cell Penetrating Peptide (CPP) Functional Family 2.B.10 The ... or Na+-translocating bacterial MotAB flagellar motor/ExbBD outer-membrane transport energizer superfamily 1.A.31 Annexin family ...

*S100A1

... , also known as S100 calcium-binding protein A1 is a protein which in humans is encoded by the S100A1 gene. S100A1 is ... Garbuglia M, Verzini M, Donato R (Sep 1998). "Annexin VI binds S100A1 and S100B and blocks the ability of S100A1 and S100B to ... S100A1 S100 calcium binding protein A1". Morii K, Tanaka R, Takahashi Y, Minoshima S, Fukuyama R, Shimizu N, Kuwano R (Feb 1991 ...
The anti-inflammatory protein annexin 1 may protect patients from the ...Severe inflammatory response syndrome or SIRS occurs when the bodys...Damazo et al. studied this balancing act by analyzing the effects of t...When normal mice were treated with LPS the immune system induced a st...The researchers characterized the expression of annexin 1 and found th...,Protein,prevents,detrimental,immune,effects,of,bacterial,sepsis,biological,biology news articles,biology news today,latest biology news,current biology news,biology newsletters
Professor Morands is a physician-scientist whose key interests are clinical outcome measurement and biological profiling in SLE, and the actions of glucocorticoid induced proteins on the immune system. Read more at https://www.monash.edu/medicine/scs/research/cid/rheumatology/researchers/eric-morand His group has pioneered the development of treat-to-target outcome measures in SLE, via the AsiaPacific Lupus Collaboration, which has over 1800 patients under study. Biomarker studies in SLE are also a major interest, with 000s of samples linked to longitudinal clinical data archived in the Australian Lupus Registry & Biobank, headquartered at Monash. His lab discovered the role in rheumatic disease of multiple important glucocorticoid-regulated proteins. Most recently his group defined the role of GILZ in RA, Th17 pathways, B cell activation, and lupus, and are working on exploiting its actions to develop a safe glucocorticoid mimic. His lab also works on innate immunity including the actions of ...
Antiphospholipid syndrome is associated with an increased risk of thrombosis and pregnancy loss. Annexin A5 (Anxa5) is a candidate autoantigen. It is not known, however, whether endogenous Anxa5 prevents foetal loss during normal pregnancy. We found
Background: Accelerated atherosclerosis is the leading cause of morbidity and mortality in diabetic patients. Hyperglycemia is a recognized independent risk factor for heightened atherogenesis in diabetes mellitus (DM). However, our understanding of the mechanisms underlying glucose damage to the vasculature remains incomplete. Methodology/Principal Findings: High glucose and hyperglycemia reduced upregulation of the NF-κB inhibitory and atheroprotective protein A20 in human coronary endothelial (EC) and smooth muscle cell (SMC) cultures challenged with Tumor Necrosis Factor alpha (TNF), aortae of diabetic mice following Lipopolysaccharide (LPS) injection used as an inflammatory insult and in failed vein-grafts of diabetic patients. Decreased vascular expression of A20 did not relate to defective transcription, as A20 mRNA levels were similar or even higher in EC/SMC cultured in high glucose, in vessels of diabetic C57BL/6 and FBV/N mice, and in failed vein grafts of diabetic patients, when ...
Rationale:. The investigators hypothesize that EPO protects against apoptosis after acute ischemia in man and that it is detectable using the annexin-A5 model.. Objective:. Does infusion of a single dose of Epoetin Alfa, a short-acting EPO, protect against apoptosis in man after acute ischemia?. Study design:. A double blinded randomised cross-over study.. Study population:. 12 Healthy male volunteers, between 18 and 40 years old.. Intervention:. All 12 volunteers will receive a single dose of EPO and placebo in a randomized order. A six week wash-out period is obtained in order to avoid interference of both treatments.. Main study parameters/endpoints:. The percentage of difference between radioactivity (quantified as counts per pixel) of the experimental and control thenar muscle at one and four hours after reperfusion. ...
A small, external bioreactor holding human cells pumped out an anti-inflammatory protein to prevent organ damage and other complications in a rat with acute inflammation caused by bacterial products in a model of sepsis, ...
Complete information for ANXA1 gene (Protein Coding), Annexin A1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Complete information for ANXA5 gene (Protein Coding), Annexin A5, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Annexin A2 is a calcium-dependent, phospholipid-binding protein found on many cell types. It consists of a short hydrophobic tail (Ser2-Asn32), which dictates its function, and a core domain (Phe33-Asp339), which is involved in phospholipid binding. Annexin A2 has been implicated in a number of biochemical processes, including cell proliferation, foetal immune tolerance, ion-channel activation, cell-cell interactions and the bridging of membranes. Annexin A2 is reported to be a powerful activator of plasminogen and, therefore, is implicated in many normal and pathological processes such as haemostasis and metastasis. Myeloid cell lines are used, extensively, to study many aspects of cellular proliferation, differentiation and function. In the present study, we have used flow cytometry and real-time PCR to investigate the role of annexin A2 expression in the proliferation and differentiation of a number of myeloid cell lines. The results demonstrated that annexin A2 expression was affected when ...
In our hands, the trypsin actually reduced the Annexin staining presumably by stripping off all the phosphotidyl-serine on the surface along with the surface proteins (adhesion molecules). The only time we have seen enhanced annexin is when the cells suffer enough trauma in harvesting that the membrane is not intact and the Annexin gets inside the cell and attaches to the ps on the inner membrane. Julie At 02:11 PM 8/18/98, you wrote: , ,I have a client who is staining with Annexin-FITC. They are looking at ,cell lines and are using trypsin to get the cells off the flasks. We are ,seeing what we think is an exagerated Annexin response. Does anyone out ,there have any feedback on how trypsin affects Annexin staining? Any ,experience and or information about this will be greatly appreciated. , Thanks in advance, , Joan Kalnitsky ,Joan Kalnitsky ,Flow Cytometry Laboratory ,VMRCVM ,(540) 231-4115 ,FAX 540-231-7367 , , It is better to serve than to receive , B. Borg , , , , , ...
Annexin A1 / ANXA1, 0.1 ml. Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane.
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Buy our Recombinant Human Annexin A13 protein. Ab105616 is a full length protein produced in Escherichia coli and has been validated in SDS-PAGE, MS. Abcam…
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BACKGROUND AND PURPOSE. Although the serum and glucocorticoid-inducible protein kinase 1 (SGK1) appears to be involved in controlling epithelial Na+ absorption, its role in this physiologically important ion transport process is undefined. As SGK1 activity is dependent upon target of rapamycin complex 2 (TORC2)-catalysed phosphorylation of SGK1-Ser(422), we have explored the effects of inhibiting TORC2 and/or TORC1 upon the hormonal control of Na+ absorption.. EXPERIMENTAL APPROACH. Na+ absorption was quantified electrometrically in mouse cortical collecting duct cells (mpkCCD) grown to confluence on permeable membranes. Kinase activities were assessed by monitoring endogenous protein phosphorylation, with or without TORC1/2 inhibitors (TORIN1 and PP242) and the TORC1 inhibitor: rapamycin.. KEY RESULTS. Inhibition of TORC1/2 (TORIN1, PP242) suppressed basal SGK1 activity, prevented insulin-and dexamethasone-induced SGK1 activation, and caused modest (10-20%) inhibition of basal Na+ absorption ...
The Annexin family of calcium-binding proteins is composed of al least thirteen mammalian genes (Annexin A1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. The Annexin family
Proteolytic cleavage of haemagglutinin (HA) is essential for the infectivity of influenza A viruses (IAVs). This is usually mediated by trypsin-like proteases present in the respiratory tract. However, the ability to use plasminogen (PLG) as an alternative protease may contribute to pathogenesis of IAV infections and virus replication outside the respiratory tract. It was demonstrated previously that neuraminidase (NA) of the IAV strain A/WSN/33 can sequester PLG, allowing this virus to replicate in a PLG-dependent fashion. However, PLG also promotes replication of other IAVs, although its mode of action is poorly understood. Here, using NA-deficient viruses, we demonstrate that NA is not required for the binding of PLG and subsequent cleavage of HA. However, we demonstrate that the cellular protein annexin 2 (A2) can bind PLG and contributes to PLG-dependent cleavage of HA and subsequent IAV replication. Collectively, these results indicate that PLG promotes IAV replication in an A2-dependent fashion
We have shown that PPAR-α agonists exert rapid and profound antinociceptive effects in animal models of acute, persistent inflammatory, and neuropathic pain, which are contingent on PPAR-α expression. These effects are comparable in time course and magnitude to those elicited by the clinically used analgesic gabapentin and are not associated with the development of tolerance. Thus, our results reveal a previously unsuspected role of PPAR-α in pain modulation and suggest that this nuclear receptor may provide a novel target for broad-spectrum analgesic drugs.. PPAR-α regulates systemic inflammation by inducing the expression of anti-inflammatory proteins, such as IκB-α, repressing the expression of proinflammatory proteins, such as tumor necrosis factor-α, and limiting the recruitment of immune cells to inflammation sites (Kostadinova et al., 2005). This array of effects is mediated through two separate but converging mechanisms-the induction of responsive target genes and the inhibition ...
ONLINE COVER A Healthy Smile (or Snarl). Periodontitis is chronic inflammation of the tissues surrounding teeth, and is not only the primary cause of tooth loss in adults, but also raises the likelihood of heart disease. Shin and colleagues discovered that an anti-inflammatory protein natural to the human body, called DEL-1, also works to prevent bone loss by acting directly on osteoclasts-the cells that resorb bone. Giving DEL-1 to mice and cynomolgus monkeys (on cover) with periodontitis stopped inflammation, restored healthy tissues, and prevented bone loss, suggesting that this endogenous protein holds therapeutic promise for gum disease and other inflammatory bone loss diseases. [CREDIT: A. SHAH/CORBIS] ...
Annexin A5 is a biomarker used in Hiv Infection, Acute Lymphoblastic Leukemia, Hypertension and 942 other diseases. Learn more about Annexin A5.
Annexin A1 Antibody 66344-1-Ig has been identified with ELISA, IF, IHC, WB. 66344-1-Ig detected 35 kDa band in A431 cells with 1:2500-1:10000 dilution...
Annexin A11 Antibody 10479-2-AP has been identified with IF, IHC, IP, WB, ELISA. 10479-2-AP detected 56-60 kDa band in HeLa cells with 1:500-1:2000 dilution...
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Purified Recombinant Human ANXA2 293 Cell Lysate from Creative Biomart. Recombinant Human ANXA2 293 Cell Lysate can be used for research.
Sigma-Aldrich offers abstracts and full-text articles by [Jiangnan Li, Dongwei Guo, Li Huang, Manman Yin, Qingfang Liu, Yan Wang, Chunmei Yang, Yuanyuan Liu, Lijie Zhang, Zhijun Tian, Xuehui Cai, Liyun Yu, Changjiang Weng].
TY - JOUR. T1 - The membrane phospholipid binding protein annexin A2 promotes phagocytosis and nonlytic exocytosis of cryptococcus neoformans and impacts survival in fungal infection. AU - Stukes, Sabriya. AU - Coelho, Carolina. AU - Rivera, Johanna. AU - Jedlicka, Anne E.. AU - Hajjar, Katherine A.. AU - Casadevall, Arturo. PY - 2016/8/15. Y1 - 2016/8/15. N2 - Cryptococcus neoformans is a fungal pathogen with a unique intracellular pathogenic strategy that includes nonlytic exocytosis, a phenomenon whereby fungal cells are expunged from macrophages without lysing the host cell. The exact mechanism and specific proteins involved in this process have yet to be completely defined. Using murine macrophages deficient in the membrane phospholipid binding protein, annexin A2 (ANXA2), we observed a significant decrease in both phagocytosis of yeast cells and the frequency of nonlytic exocytosis. Cryptococcal cells isolated from Anxa2-deficient (Anxa2-/-) bone marrow-derived macrophages and lung ...
Using biochemical, epifluorescence and electron microscopic techniques in a U937 model system, we investigated the effect of anti-allergic drugs di-sodium cromoglycate and sodium nedocromil on the trafficking and release of the anti-inflammatory protein Annexin-A1 (Anx-A1) when this was triggered by glucocorticoid (GC) treatment. GCs alone produced a rapid (within 5min) concentration-dependent activation of PKCalpha/beta (Protein Kinase C; EC 2.7.11.13) and phosphorylation of Anx-A1 on Ser(27). Both phosphoproteins accumulated at the plasma membrane and Anx-A1 was subsequently externalised thereby inhibiting thromboxane (Tx) B(2) generation. When administered alone, cromoglycate or nedocromil had little effect on this pathway however, in the presence of a fixed sub-maximal concentration of GCs, increasing amounts of the cromoglycate-like drugs caused a striking concentration-dependent enhancement of Anx-A1 and PKCalpha/beta phosphorylation, membrane recruitment and Anx-A1 release from cells ...
The role of the calcium- and phospholipid-binding protein annexin I (ANXA1) in cell cycle regulation has been investigated in estrogen receptor (ER)-positive MCF-7 and ER-negative MDA-MB-231 breast tumor cell lines. In MCF-7 cells, ANXA1-targeting small interfering RNA (siRNA) reduced ANXA1 mRNA and protein levels and attenuated cell proliferation induced by FCS, estradiol, or epidermal growth factor. Well-characterized agonists for the known ANXA1 receptor, FPR2, including the ANXA1 N-terminal proteolytic product ANXA1(2-26), lipoxin A(4) (LXA(4)), and the synthetic peptide, Trp-Lys-Tyr-Met-Val-D-Met (WKYMVm), stimulated proliferation of MCF-7 and MDA-MB-231 cells that was attenuated by incubation with FPR2 antagonists WRW(4) (1 μM) or Boc2 (100 nM) or by siRNA against FPR2. FCS-induced mitogenic responses were attenuated by each of the FPR antagonists and by siRNA against FPR2 and, to a lesser extent, FPR1. LXA(4) increased phosphorylation of Akt, p70(S6K) but not ERK1/2. Increases in cyclin ...
Mouse anti Human Annexin A3 antibody, clone 4F1 recognizes human annexin A3, also known as Annexin III, Inositol 1,2-cyclic phosphate 2-ph
Annexin A1 is an intracellular calcium/phospholipid-binding protein that is involved in membrane organization and the regulation of the immune system. It has been attributed an anti-inflammatory role at various control levels, and recently we could show that annexin A1 externalization during secondary necrosis provides an important fail-safe mechanism counteracting inflammatory responses when the timely clearance of apoptotic cells has failed. As such, annexin A1 promotes the engulfment of dying cells and dampens the postphagocytic production of proinflammatory cytokines. In our current follow-up study, we report that exposure of annexin A1 during secondary necrosis coincided with proteolytic processing within its unique N-terminal domain by ADAM10. Most importantly, we demonstrate that the released peptide and culture supernatants of secondary necrotic, annexin A1-externalizing cells induced chemoattraction of monocytes, which was clearly reduced in annexin A1- or ADAM10-knockdown cells. Thus, ...
Abbreviations: BLMEC, bovine lung microvascular endothelial cell; DUSP, dual-specificity phosphatase; E, embryonic day; eNOS, endothelial nitric oxide synthase; ERK, extracellular-signal-regulated kinase; FGF2, fibroblast growth factor 2; HDMEC, human dermal microvascular endothelial cell; HUVEC, human umbilical vein endothelial cell; KLF, Krüppel-like factor; MAPK, mitogen-activated protein kinase; MAPKK, MAPK kinase; MAPKKK, MAPKK kinase; MEF, myocyte enhancer factor; MEK, MAPK/ERK kinase; MEKK, MEK kinase; MKP, MAPK phosphatase; NF-κB, nuclear factor κB; NLS, nuclear localization signal; NRF2, nuclear erythroid 2-related factor 2; PKB, protein kinase B; RSK, ribosomal S6 kinase; SGK, serum- and glucocorticoid-regulated protein kinase; TNFα, tumour necrosis factor α; VCAM-1, vascular cell adhesion molecule 1; VEGF, vascular endothelial growth factor ...
Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. The alternative splicing event is tissue specific and the mRNA containing the cassette exon is prevalent in brain, heart and skeletal muscle. The transcripts also differ in their 3-non coding regions by the use of two alternative poly(A) signals. Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, ...
Purpose: : To examine the role of annexin 2 in the formation and internalisation of phagosomes in retinal pigment epithelial (RPE) cells. Methods: : Human ARPE19 cells were cultured on the glass inserts of Matek dishes for immunofluorescence and live imaging, or plastic Nunc dishes for biochemical analysis. In some studies ARPE19 cells were exposed to siRNA for annexin 2 for three days prior to experimentation. Outer segments were prepared from porcine retina using standard protocols (typically 50 - 100 eyes per prep), and either used fresh in biochemical experiments, or labelled with an appropriate fluorescent marker prior to imaging experiments. Results: : Depletion of annexin 2 using siRNA led to a significant quantitative reduction in the phagocytosis of rod outer segments in cultured RPE cells. In cells expressing annexin 2, imaged by confocal microscopy, a striking enrichment of annexin 2 and F-actin was observed at the phagocytic cup immediately upon engagement of the apical cell surface ...
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis. Alternatively spliced transcript variants have been described. [provided by RefSeq, Aug 2010 ...
The ubiquitin E3 protein ligase Nedd4-2 is a physiological regulator of the epithelial sodium channel ENaC, which is essential for transepithelial Na+ transport and is linked to Liddles syndrome, an autosomal dominant disorder of human salt-sensitive hypertension. Nedd4-2 function is negatively regulated by phosphorylation via a serum- and glucocorticoid-inducible protein kinase (Sgk1), which serves as a mechanism to inhibit the ubiquitination-dependent degradation of ENaC. We report here that 14-3-3 proteins participate in this regulatory process through a direct interaction with a phosphorylated form of human Nedd4-2 (a human gene product of KIAA0439, termed hNedd4-2). The interaction is dependent on Sgk1-catalyzed phosphorylation of hNedd4-2 at Ser-468. We found that this interaction preserved the activity of the Sgk1-stimulated ENaC-dependent Na+ current while disrupting the interaction decreased ENaC density on the Xenopus laevis oocytes surface possibly by enhancing Nedd4-2-mediated ...
|p|Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry
In the present study, we show that both CRP and annexin A5 specifically bind to oxidized LDL. Via coupling to Fcγ receptors on macrophages and independent of complement, CRP subsequently enhances the association of oxidized LDL to macrophages. Enhanced association of oxidized LDL to macrophages via CRP-Fcγ receptor interaction may lead to enhanced macrophage uptake of oxidized LDL. Moreover, we show that annexin A5 binds to a site at oxidized LDL different from the CRP binding site and that annexin A5 does not antagonize the CRP effect.. Previously, Chang et al also observed specific binding of CRP to oxidized LDL,7 characterized by inhibition by whole molecules as well as F(ab′)2 fragments of EO6, an IgM antibody that binds specifically to the phosphorylcholine head group of oxidized phospholipids but not to the same moiety on nonoxidized phospholipids.26 In the present study, for characterization of binding sites, we used the monoclonal antibody DLH3 (also IgM), which recognizes an epitope ...
Annexin A6 (AnxA6) has been implicated in the regulation of endo-/exocytic pathways, cholesterol transport, and the formation of multifactorial signaling complexes in many different cell types. More recently, AnxA6 has also been linked to triglyceride storage in adipocytes. Here we investigated the potential role of AnxA6 in fatty acid (FA) induced lipid droplet (LD) formation in hepatocytes. AnxA6 was associated with LD from rat liver and HuH7 hepatocytes. In oleic acid (OA) -loaded HuH7 cells, substantial amounts of AnxA6 bound to LD in a Ca2+-independent manner. Remarkably, stable or transient AnxA6 overexpression in HuH7 cells led to elevated LD numbers/size and neutral lipid staining under control conditions as well as after OA loading compared to controls. In contrast, overexpression of AnxAl, AnxA2 and AnxA8 did not impact on OA-induced lipid accumulation. On the other hand, incubation of AnxA6-depleted HuH7 cells or primary hepatocytes from AnxA6 KO-mice with OA led to reduced FA ...
Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 has 45 to 59% identity with other members of its family and shares a similar size and exon-intron organization. Isolated from human placenta, ANX4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells ...
Annexin A2 (AnxA2) is a multi-functional and -compartmental protein whose subcellular localisation and functions are tightly regulated by its post-translational modifications. AnxA2 and its Tyr23-phosphorylated form (pTyr23AnxA2) are involved in malignant cell transformation, metastasis and angiogenesis. Here, we show that H2O2 exerts rapid, simultaneous and opposite effects on the Tyr23 phosphorylation status of AnxA2 in two distinct compartments of rat pheochromocytoma (PC12) cells. Reactive oxygen species induce dephosphorylation of pTyr23AnxA2 located in the PML bodies of the nucleus, whereas AnxA2 associated with F-actin at the cell cortex is Tyr23 phosphorylated. The H2O2-induced responses in both compartments are transient and the pTyr23AnxA2 accumulating at the cell cortex is subsequently incorporated into vesicles and then released to the extracellular space. Blocking nuclear export by leptomycin B does not affect the nuclear pool of pTyr23AnxA2, but increases the amount of total AnxA2 ...
1HVE: Structural and electrophysiological analysis of annexin V mutants. Mutagenesis of human annexin V, an in vitro voltage-gated calcium channel, provides information about the structural features of the ion pathway, the voltage sensor and the ion selectivity filter
Annexin II antibody (annexin A2) for ICC/IF, IHC-P, IP, RipA, WB. Anti-Annexin II pAb (GTX32446) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
Annexin A1 antibody (annexin A1) for ICC/IF, IHC-P, IP, WB. Anti-Annexin A1 pAb (GTX101070) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
The atherosclerotic process is considered to be driven by an imbalance between proand anti-inflammatory actions. Still, the inflammatory state in patients with coronary artery disease (CAD) remains to be clarified. Annexin A1 (AnxA1) is a glucocorticoidinduced protein which may have a key role in the anti-inflammatory response as a mediator of glucocorticoid effects.. The general aim of this thesis was to deepen the knowledge of pro- and antiinflammatory mechanisms in CAD via phenotypic assessments of immune cell subsets, in particular CD56+ T cells, and exploration of AnxA1. The long-term goal is to reveal basic mechanisms that will lead to the development of biomarkers, which may be used for individualized treatment and monitoring.. The AnxA1 protein was constitutively expressed in both neutrophils and peripheral blood mononuclear cells (PBMCs). However, it varied considerably across PBMC subsets, being most abundantly expressed in monocytes. The AnxA1 expression was also higher in CD56+ T ...
Annexin V is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Annexin V is a monomer, binds ATRX and EIF5B, and interacts with hepatitis B virus (HBV ...
BioAssay record AID 590043 submitted by ChEMBL: Induction of apoptosis in human K562 cells assessed as viable cells at 50 nM using annexin V-propidium iodide and annexin V staining by flow cytometry (Rvb = 87.02%).
ANXA2, a member of the annexin family, is a calcium-dependent phospholipid-binding protein that plays a role in the regulation of cellular growth and in signal transduction pathways. Its affinity for calcium is greatly enhanced by anionic phospholipids, and it binds two calcium ions with high affinity. ANXA2 may be involved in heat-stress response. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. It may also cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis.
Order monoclonal and polyclonal Annexin A13 antibodies for many applications. Selected quality suppliers for anti-Annexin A13 antibodies.
Buy ANXA2 recombinant protein, Annexin A2 Recombinant Protein-NP_001002857 (MBS203687) product datasheet at MyBioSource, Recombinant Proteins. Application: SDS-PAGE
SCAN HISTORY OWL11_0 2 100 NSINGLE OWL17_1 2 110 NSINGLE OWL18_0 1 115 NSINGLE OWL19_1 1 125 NSINGLE OWL21_1 1 125 NSINGLE OWL26_0 1 125 NSINGLE SPTR37_9f 2 237 NSINGLE INITIAL MOTIF SETS ANNEXIN1 Length of motif = 23 Motif number = 1 Annexin motif I - 1 PCODE ST INT KTKGVDEVTIVNILTNRSNAQRQ LUHU36 46 46 KTKGVDEVTIINILTNRSNEQRQ ANX2_CHICK 46 46 TVKGVDEATIIDILTKRNNAQRQ ANX1_CAVCU 56 56 MVKGVDEATIIDILTKRNNAQRQ LUHU 55 55 KGIGTDEATIIDIVTHRSNAQRQ ANX6_MOUSE 376 376 KGMGTDEETILKILTSRNNAQRQ ANX5_CHICK 29 29 KGIGTNEQAIIDVLTKRSNTQRQ ANX8_HUMAN 35 35 KGFGTDEQEIIDVLVGRSNQQRQ DROANNX 29 29 RGIGTDEKMLISILTERSNAQRQ ANX3_HUMAN 32 32 KGLGTDEDAIINVLAYRSTAQRQ ANX4_BOVIN 27 27 KGFGTDEQAIVDVVANRSNDQRQ HUMSNEXIN 177 177 TAKGVDEATIIDIMTTRTNAQRP ANX1_COLLI 51 51 ANNEXIN2 Length of motif = 17 Motif number = 2 Annexin motif II - 1 PCODE ST INT LKSALSGHLETVILGLL LUHU36 86 17 LKSALSGHLEAVILGLL ANX2_CHICK 86 17 LKKALTGHLEEVVLALL ANX1_CAVCU 96 17 LKKALTGHLEEVVLALL LUHU 95 17 LKSEISGDLARLILGLM ANX6_MOUSE 416 17 ...
Abcam provides specific protocols for Anti-Annexin A10 antibody (ab2343) : Western blot protocols, Immunohistochemistry protocols
1HVD: Structural and electrophysiological analysis of annexin V mutants. Mutagenesis of human annexin V, an in vitro voltage-gated calcium channel, provides information about the structural features of the ion pathway, the voltage sensor and the ion selectivity filter
BioAssay record AID 502910 submitted by ChEMBL: Induction of annexin 2-mediated cytoskeleton aggregate accumulation in human HepG2 cells at 4 uM after 2 hrs by confocal laser scanning microscopy.
Bachem offers H-3196 Annexin A1 (1-25) (dephosphorylated) (human) for your research. Find all specific details here. Find product specific information including available pack sizes, CAS, detailed description and references here.
Mouse Monoclonal Anti-Annexin A3 Antibody (2F3). Validated: WB, ICC/IF, IHC, IHC-P. Tested Reactivity: Human, Monkey. 100% Guaranteed.
Purified Recombinant Mouse Anxa1 Protein, His-tagged from Creative Biomart. Recombinant Mouse Anxa1 Protein, His-tagged can be used for research.
Annexin A2 is a calcium regulated protein involved in membrane binding and actin polymerization. In this paper, Kevin Moreau and colleagues identified Annexin A2 as a…. ...
It has been reported that Annexin A2 (ANXA2) is up-regulated in hepatocellular carcinoma (HCC), but the roles of ANXA2 in the migration and invasion of HCC ...
Expression of ANXA6 (ANX6) in cancer tissue. The cancer tissue page shows antibody staining of the protein in 20 different cancers.
Human ANXA2 full-length ORF ( AAH09564.1, 1 a.a. - 339 a.a.) recombinant protein with GST-tag at N-terminal. (H00000302-P01) - Products - Abnova
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www.ncbi.nlm.nih.gov/pmc/articles/PMC3063939. Our results indicate that 4-1BB co-stimulation may significantly improve TIL survival during ... mAb from Bristol Myers Squibb (BMS) currently being tested in clinical trials,. ... BMS-663513 was at 14.9 mg/ml and was stored at 4°C. This Ab was .... The cells were then stained with anti-CD8-Pacific Blue, Annexin V-PE, and .... ...
Fmoc-D-Lys(Boc)-OHNα-9-Fluorenylmethoxycarbonyl-Nε-t-butyloxycarbonyl-D-lysine | Fmoc-D-Lys(Boc)2411 1 g | 95.00 EUR 2411 5 g ...
Annexin A1, also known as lipocortin I, is a protein that is encoded by the ANXA1 gene in humans. Annexin A1 belongs to the annexin family of Ca2+-dependent phospholipid-binding proteins that have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin A1 protein has an apparent relative molecular mass of 40 kDa with phospholipase A2 inhibitory activity. Glucocorticoids (such as budesonide, cortisol, and beclomethasone) are a class of endogenous or synthetic anti-inflammatory steroid hormones that bind to the glucocorticoid receptor (GR), which is present in almost every vertebrate animal cell. They are used in medicine to treat diseases caused by an overactive immune system, including allergies, asthma, autoimmune diseases, and sepsis. Because they suppress inflammatory pathways, long-term use of glucocorticoid drugs can lead to side-effects such as immunodeficiency and adrenal insufficiency. The main mechanism of ...
The surface of healthy cells is composed of lipids that are asymmetrically distributed on the inner and outer leaflet of the plasma membrane. One of these lipids, phosphatidylserine (PS), is normally restricted to the inner leaflet of the plasma membrane and is, therefore, only exposed to the cell cytoplasm. However, during apoptosis lipid asymmetry is lost and PS becomes exposed on the outer leaflet of the plasma membrane. Annexin V, a 36-kDa calcium-binding protein, binds to PS; therefore, fluorescently labeled Annexin V can be used to detect PS that is exposed on the outside of apoptotic cells. Annexin V can also stain necrotic cells because these cells have ruptured membranes that permit Annexin V to access the entire plasma membrane. However, apoptotic cells can be distinguished from necrotic cells by co-staining with propidium iodide (PI) because PI enters necrotic cells but is excluded from apoptotic cells. This protocol describes Annexin V binding and PI uptake followed by flow cytometry ...
Annexin A1 (ANXA1) is a calcium- and phospholipid-binding protein and a known mediator of glucocorticoid-regulated inflammatory responses. Using a combined multiple high-throughput approach, we recently identified a reduced expression of ANXA1 in human breast cancer. The finding was confirmed at the gene level by quantitative reverse transcription-polymerase chain reaction and at the protein level by immunohistochemical staining of normal, benign, and malignant breast tissues. In this study, we constructed and used a high-density human breast cancer tissue microarray to characterize the expressional pattern of ANXA1 according to histopathologies. The tissue microarray contains 1,158 informative breast tissue cores of different histologies including normal tissues, hyperplasia, in situ and invasive tumors, and lymph node metastases. Our results showed that there was a significant decrease in glandular expression of ANXA1 in ductal carcinoma in situ and invasive ductal carcinoma compared with ...
Helen Christians research interests are in the mechanisms of steroid hormone regulation of the pituitary gland, in particular the role of Annexin 1. The Annexins are a well-conserved super-family of structurally related Ca2+ - and phospholipids-binding proteins with wide-ranging functions in health and disease. Annexin 1 is a 37kD protein that is induced by glucocorticoids and mediates glucocorticoid action within the host defence and neuroendocrine systems. Glucocorticoids regulate the synthesis, phosphorylation and cellular disposition of annexin 1, and annexin 1 is implicated in the regulation by these important drugs of pituitary function, the control of cell growth and signal transduction. Most recently her group have characterized a novel secretory pathway of annexin 1, a protein which lacks a signal sequence, involving a member of the ATP binding cassette transporter family, ABCA1. This is the first time the secretion mechanism of an annexin family member has been determined and the ...
TransDetect® Annexin V-EGFP/PI Cell Apoptosis Detection Kit,Cell Detection,Cell Culture and Detection,Products,Beijing TransGen Biotech Co.Ltd,OverviewContents& storageCitations & referencesRelated ImagesDownloadOverviewDescriptionThe Annexin V
There are many different types of joint pain all of which can be extremely uncomfortable and sometimes even debilitating. These include auto-immune conditions such as rheumatoid arthritis and lupus, osteoarthritis, fibromyalgia, gout, tendonitis, bursitis, overuse injuries and much more.. Common signs associated with these conditions are lessened quality of life, aching joints, pain with movement, decreased mobility, swelling and soreness to the touch. There also tends to be a high correlation with these conditions and reliance on anti-inflammatory and other types of pain medication.. So how can whole body cryotherapy help? With the brief (but comfortable!) exposure to extreme cold your body creates a physiological reaction that will positively affect your joints and other parts of the body. Specifically, it causes an immediate release within your body of anti-inflammatory proteins and endorphins as well as increased circulation to the joints. As this happens, joint pain is reduced (or even ...
A stem cell bone marrow aspirate concentrate is a component of your bone marrow that contains growth factors and anti-inflammatory proteins
Dr. RJ Tesi, CEO of FPRT Biosciences, led the session describing his companys clinical stage protein-based therapy, XPro1595, for the treatment of neurodegenerative diseases including Alzheimers diseases, Parkinsons disease, multiple sclerosis, ALS, and Huntingtons disease. XPro1595 is a blood-brain penetrant anti-inflammatory protein that promotes neuronal survival by treating a single pathology common across many neurodegenerative diseases, excessive neuroinflammation. How does the protein work? XPro1595 is a 17kD "kissing cousin" of soluble TNFα (XPro1595 has six amino acid substitutions as compared to the wild-type protein). The minor changes in sequence gives XPro1595 a therapeutic advantage that in Tesis words is "unmatched"; XPro1595 has the ability to selectively bind and neutralize soluble TNFα, while not affecting transmembrane TNFα. XPro1595 sequesters soluble TNFα blocking downstream receptor-ligand interactions that would normally activate an inflammatory response. FPRT ...
Annexin A6 (AnxA6) is a Ca2+-dependent membrane-binding protein involved in vesicular traffic. The likely participation of AnxA6 in the response of lymphocytes to Ca2+ signals has not been investigated yet. The present study focuses on intracellular relocation of AnxA6 in human Jurkat T lymphoblasts upon stimulation followed by transient increase of intracellular [Ca2+] and exocytosis of interleukin-2 (IL-2). Stimulation of the cells under different experimental conditions (by lowering pH and/or by rising extracellular [Ca2+] in the presence of ionomycin) induced time-dependent transients of intracellular [Ca2+] and concomitant changes in AnxA6 intracellular localization and in IL-2 secretion, with only minor effects on cell viability and apoptosis. In resting conditions (in the presence of EGTA or with no ionophore) AnxA6 was localized uniformly in the cytosol, whereas it translocated to vesicular structures beneath the plasma membrane within 5 min following stimulation of Jurkat T cells and ...
ELISA method for the measurement of IgM isotype auto antibodies targeted to annexin V. Assay designed with highly purified human recombinant annexin V, obtained with non-denaturing methods, for auto antibody immunocapture. These are then revealed with a goat antibody specific for IgM, micro fragments, and labeled with peroxidase.
For those of you who were following the Caspase-3 thread and those of you who contacted me directly, heres the information regarding Caspase-3 and Annexin V that I received from Pharmingen. Any furthur questions, please contact Padma at Pharmingen. Padma was most helpful. Lora , -----Original Message----- , From: pkodukula at pharmingen.com [SMTP:pkodukula at pharmingen.com] , Sent: Monday, October 04, 1999 10:14 AM , To: Barsky, Lora , Subject: RE: caspase-3 , , , , Dear Lora, , , Thank you for your interest in PharMingens products. This is in reply to , your , question about the caspase 3 and the annexin V staining. We are releasing , a kit , for the BrDU and cytokine staining. But the annexin V is also known to , work , with the caspase 3. This is not something that we routinely quality , control in , our company but we have had input from customers who have used this , method. The , annexin V binding to phosphatidyl serine is dependent on the CA++ ion , concentration and so a Ca++ ...
Auranofin promotes mitochondrial apoptosis by inducing annexin A5 expression and translocation in human prostate cancer cells.: Auranofin is a lipophilic gold c
Anexina A3 é uma proteína que nos humanos é codificada pela gene ANXA3. É expressada de maneira anormal em fetos derivados de fertilização in vitro e de microinjecção intracitoplasmática, podendo contribuir para um risco aumentado de defeitos no recém-nascido nestas tecnologias de reprodução medicamente assistida. Este gene codifica um membro da família das anexinas. Os membros desta família de proteínas ligadas aos fosfolipídios dependentes de cálcio desempenham um papel na regulação do crescimento celular e em vias de transdução de sinal. Esta proteína funciona na inibição da fosfolipase A2 e na clivagem de inositol 1,2-fosfato cíclico para formar inositol 1-fosfato. Também desempenha um papel na anti-coagulação. Tait JF, Frankenberry DA, Miao CH, Killary AM, Adler DA, Disteche CM (agosto de 1991). «Chromosomal localization of the human annexin III (ANX3) gene». Genomics. 10 (2): 441-8. PMID 1830024. doi:10.1016/0888-7543(91)90330-H !CS1 manut: Nomes múltiplos: ...
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Mouse Monoclonal Anti-ANXA1 Antibody against Human annexin A1. Validated for Immunofluorescence, Immunohistochemistry and Western Blot
Rabbit polyclonal antibody raised against partial recombinant ANXA1. Recombinant protein corresponding to amino acids 35-346 of human ANXA1. (PAB18835) - Products - Abnova
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Sigma-Aldrich offers abstracts and full-text articles by [Jakob Voelkl, Ioana Alesutan, Tatsiana Pakladok, Robert Viereck, Martina Feger, Sobuj Mia, Tanja Schönberger, Angelika A Noegel, Meinrad Gawaz, Florian Lang].
Expression of ANXA13 (ANX13) in oral mucosa tissue. Antibody staining with HPA018535, HPA019569, HPA019650 and CAB025135 in immunohistochemistry.
h2,Quelles sont les causes des troubles du comportement?,/h2, ,p,Les troubles du comportement peuvent être causés par:,/p, ,ul,,li,facteurs biologiques;,/li, ,li,facteurs sociaux et environnementaux;,/li, ,li,facteurs psychologiques.,/li,,/ul, ,h3,Facteurs biologiques,/h3, ,p,Certains traits caractéristiques des troubles du comportement sont parfois héréditaires. Les enfants dont la famille a souffert de troubles comportementaux ou dapprentissage, d,a href="/Article?contentid=18&language=French",anxiété,/a,, de ,a href="/Article?contentid=19&language=French",dépression,/a, ou dune ,a href="/Article?contentid=279&language=French",psychose bipolaire,/a, courent peut-être plus de risques de présenter des troubles du comportement.,/p, ,h3,Facteurs sociaux et environnementaux,/h3, ,p,Les enfants dont la famille est très stressée sont sans doute plus susceptibles de présenter des symptômes de trouble comportemental. Voici certains des facteurs qui stressent les familles:,/p, ...
The discovery of an essential role for annexin 11 in cell division is unexpected when considered in the broader context of annexin biology. Although annexins are now firmly implicated in various cellular activities, including endocytosis, calcium signaling, and apoptosis, no member of this protein family has been demonstrated to function in the cell cycle. The membrane binding and vesicle fusogenic properties that define annexins in biochemical terms could provide clues as to the possible role of annexin 11 in the last stage of cytokinesis. Before cells can be completely separated in a process known as abscission, the actomyosin constriction ring at the cleavage furrow has to be disassembled and the central spindle must separate. During this series of events, it is likely that the plasma membrane must be tightly attached to the midbody microtubules for abscission to be successfully completed. CHO1 has previously been suggested to be responsible for this connection (Kuriyama et al., 2002), but ...
Membrane repair is essential to cell survival. In skeletal muscle, injury often associates with plasma membrane disruption. Additionally, muscular dystrophy is linked to mutations in genes that produce fragile membranes or reduce membrane repair. Methods to enhance repair and reduce susceptibility to injury could benefit muscle in both acute and chronic injury settings. Annexins are a family of membrane-associated Ca2+-binding proteins implicated in repair, and annexin A6 was previously identified as a genetic modifier of muscle injury and disease. Annexin A6 forms the repair cap over the site of membrane disruption. To elucidate how annexins facilitate repair, we visualized annexin cap formation during injury. We found that annexin cap size positively correlated with increasing Ca2+ concentrations. We also found that annexin overexpression promoted external blebs enriched in Ca2+ and correlated with a reduction of intracellular Ca2+ at the injury site. Annexin A6 overexpression reduced membrane ...
Gliomas are highly invasive brain tumors with the occurrence of numerous microglia/macrophages (MG/MP) in and around the tumor. Annexin A2 is overexpressed in many cancers and correlates with increased plasmin activity on the tumor cell surface. Plasmin mediates degradation of extracellular matrix and angiogenesis to facilitate tumor growth. In this study, we used a mouse glioma cell line, GL261-EGFP, and stable clones transfected with an annexin A2 knockdown (annA2KD) construct, GL261-EGFP-annA2KD. We found that the annA2KD decreased glioma cell migration in vitro and decreased membrane-bound plasmin activity. In vivo we injected GL261-EGFP cells into the mouse brain and the glioma progression was followed. Knockdown of annexin A2 in glioma cells decreased tumor size and slowed down tumor progression, characterized by decreased invasion, angiogenesis and proliferation, as well as increased apoptosis in tumor tissue of the annA2KD group. We also investigated the interaction between glioma and ...
The pHi (intracellular pH) is an important physiological parameter which is altered during hypoxia and ischaemia, pathological conditions accompanied by a dramatic decrease in pHi. Sensors of pHi include ion transport systems which control intracellular Ca2+ gradients and link changes in pHi to functions as diverse as proliferation and apoptosis. The annexins are a protein family characterized by Ca2+-dependent interactions with cellular membranes. Additionally, in vitro evidence points to the existence of pH-dependent, Ca2+-independent membrane association of several annexins. We show that hypoxia promotes the interaction of the recombinant annexin A2-S100A10 (p11) and annexin A6 with the plasma membrane. We have investigated in vivo the influence of the pHi on the membrane association of human annexins A1, A2, A4, A5 and A6 tagged with fluorescent proteins, and characterized this interaction for endogenous annexins present in smooth muscle and HEK (human embryonic kidney)-293 cells ...
Invading pathogens may trigger overactivation of the innate immune system, which results in the release of large amounts of proinflammatory cytokines (cytokine storm) and leads to the development of pulmonary edema, multiorgan failure, and shock. PIAS1 is a multifunctional and potent anti-inflammatory protein that negatively regulates several key inflammatory pathways such as Janus kinase (JAK)-signal transducer and activator of transcription (STAT) and nuclear factor κB (NF-κB). We discovered a ubiquitin E3 ligase, HECTD2, which ubiquitinated and mediated the degradation of PIAS1, thus increasing inflammation in an experimental pneumonia model. We found that GSK3β phosphorylation of PIAS1 provided a phosphodegron for HECTD2 targeting. We also identified a mislocalized HECTD2 polymorphism, HECTD2A19P, that was present in 8.5% of the population and functioned to reduce inflammation. This polymorphism prevented HECTD2/PIAS1 nuclear interaction, thus preventing PIAS1 degradation. The HECTD2A19P ...
It is thought that the involvement of common mediators such as corticotrophin releasing hormone (CRR), cytokines, prostaglandins (PGs), pituitary peptides and glucocorticoids allows the integration of immune and neuroendocrine activity during the acute phase response (APR) to injury and infection. Glucocorticoids are the major inhibitory component within this complex system, which has been termed the immunehypothalamic- pituitary-adrenal (HPA) axis. In the past, lipocortin-1 (LC-1) has been implicated as a "second messenger" of glucocorticoid actions. The present study explored this potential role for LC-l in relation to the immune-HPA axis ...
The histamine H2-receptor (H2R) is a Gs protein-coupled receptor. Its activation leads to increases in the second messenger adenosine-3,5-cyclic monophosphate (cAMP). Presently, several systems are established to characterize the pharmacological profile of the H2R, mostly requiring radioactive material, animal models or human blood cells. This prompted us to establish a flow cytometric analysis with a fluorescently labeled formyl peptide receptor (FPR) ligand in order to investigate the H2R functionally and pharmacologically. First, we stimulated U937 promonocytes, which mature in a cAMPdependent fashion upon H2R activation, with histamine (HA) or selective H2R agonists and measured increases in cAMP concentrations by mass spectrometry. Next, indicative for the maturation of U937 promonocytes, we assessed the FPR expression upon incubation with HA or H2R agonists. FPR expression was measured either indirectly by formyl peptide-induced changes in intracellular calcium concentrations ([Ca2+]i) ...
A idea behind this research is: if we know what parts of the bacteria stimulate healing, perhaps doctors can deliver that material, or something very close, to patients directly to treat intestinal diseases such as Crohns or ulcerative colitis.. This idea has advanced experimentally, as demonstrated by two papers from Jones and Neish’s frequent collaborator, Asma Nusrat, who recently moved from Emory to the University of Michigan. This team had shown that a protein produced by human intestinal cells called annexin A1 activates ROS, acting through the same N-formyl peptide receptors that bacteria do.. Nusrat told me Friday her team began investigating annexins a decade ago at Emory, and it was fortuitous that Neish was working on beneficial bacteria right down the hall, since it is now apparent that annexin A1 and the bacteria are activating the same molecular signals. (Did you know there is an entire conference devoted to annexins? I didnt until a few days ago.). In a ...
Hepatocellular carcinoma (HCC) is a common tumor worldwide with an extremely poor prognosis. Alpha-fetoprotein (AFP) is the only available HCC serological biomarker and has limited effect at early stages. Previous work has proven that AFP-producing and -nonproducing HCC cells have different gene/protein expression profiles. To identify additional candidates that might aid in HCC diagnosis, in addition to giving a better understanding of the mechanisms underlying hepatocarcinogenesis, subcellular fraction proteins from multiple liver cancer (HepG2, Hep3B and SK-HEP-1) and normal cells (HL-7702) were separated and identified using a 2-DE/MALDI-TOF-TOF approach. In total, 50 differentially-expressed proteins between liver cancer and normal liver cells were identified from different fractions including the cytosol, membrane/organelles and nuclear proteins. Among them, the tumor-related protein Annexin A2, was verified to be significantly upregulated in HCC tissues (63.5%, 80/126) especially in ...
Page contains details about annexin V conjugated to maleimide-functionalized Feraheme nanoparticles . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Annexin V-FITC Apoptosis assay results: Flow cytometry profiles represent Annexin-V-FITC staining in X-axis and PI in Y-axis: (a) control, i.e., neither PSi nor
TY - JOUR. T1 - Annexins family. T2 - Insights into their functions and potential role in pathogenesis of sarcoidosis. AU - Mirsaeidi, Mehdi. AU - Gidfar, Sanaz. AU - Vu, Ann. AU - Schraufnagel, Dean. PY - 2016. Y1 - 2016. N2 - Annexins are Ca2+-regulated phospholipid-binding proteins that play an important role in the cell life cycle, exocytosis, and apoptosis. Annexin A11 is one of the oldest vertebrate annexins that has a crucial role in sarcoidosis pathogenesis. The mechanism of effect in sarcoidosis granuloma cells may be due to alterations in apoptosis. Immune cells with a specific mutation at protein location 230 are resistant to apoptosis and consequently have continued effects on inflammation and progression of sarcoidosis. The mechanism of action of annexin A11 may be based upon alterations in delivering calcium to two different apoptosis pathways (caspase and P53).. AB - Annexins are Ca2+-regulated phospholipid-binding proteins that play an important role in the cell life cycle, ...
The N-formyl peptide receptors (FPRs) are a family of G-protein coupled receptors that respond to proinflammatory N-formylated bacterial peptides (e.g., formyl-Met-Leu-Phe, fMLF) and, thus, contribute to the host response to bacterial infection. Paradoxically, a growing body of evidence suggests that some members of this receptor family may also be targets for certain anti-inflammatory molecules, including annexin A1 (ANXA1), which is an important mediator of glucocorticoid (GC) action. To explore further the potential role of FPRs in mediating ANXA1 actions, we have focused on the pituitary gland, where ANXA1 has a well-defined role as a cell-cell mediator of the inhibitory effects of GCs on the secretion of corticotrophin (ACTH), and used molecular, genetic, and pharmacological approaches to address the question in well-established rodent models. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis identified mRNAs for four FPR family members in the mouse anterior pituitary gland, Fpr-rs1
Apoptosis, or programmed cell death, is a general mechanism for removal of unwanted cells from the immune system. It is characterized by chromatin condensation, a reduction in cell volume, and endonuclease cleavage of DNA into oligonucleosomal length fragments. Apoptosis is also accompanied by a loss of membrane phospholipid asymmetry, resulting in the exposure of phosphatidylserine at the surface of the cell. Expression of phosphatidylserine at the cell surface plays an important role in the recognition and removal of apoptotic cells by macrophages. Here we describe a new method for the detection of apoptotic cells by flow cytometry, using the binding of fluorescein isothiocyanate-labeled annexin V to phosphatidylserine. When Burkitt lymphoma cell lines and freshly isolated germinal center B cells are cultured under apoptosis inducing conditions, all cells showing chromatin condensation strongly stain with annexin V, whereas normal cells are annexin V negative. Moreover, DNA fragmentation is ...
Annexin A1 (AnxA1), a phospholipid-binding protein and known regulator of glucocorticoid-induced inflammatory signaling, has been implicated for its role in human cancers, including head and neck cancer, colon, breast and prostate cancers. To determine the role of AnxA1 found to be secreted by the cancer-associated fibroblasts (CAF) of prostatic adenocarcinoma, we used primary cultures of CAFs, primary epithelial cultures and an epithelial cell line (cE1), all derived from the cPten-/-L mouse model of prostate adenocarcinoma in a series of experiments described here. The results of this dissertation point to a novel function of AnxA1 in prostate tumorigenesis and the establishment of a Cancer Cell-EMT-CSC lineage. It is projected that the presence of AnxA1 in the tumor microenvironment contributes to tumor stem cell activity via two separate but complementary pathways, first by induction of a de-differentiation process leading to generation of basal stem-like cells from a subpopulation of cancer ...
The application of fluid shear stress on leukocytes is critical for physiological functions including initial adhesion to the endothelium, the formation of pseudopods, and migration into tissues. The formyl peptide receptor (FPR) on neutrophils, which binds to formyl-methionyl-leucyl-phenylalanine (fMLP) and plays a role in neutrophil chemotaxis, has been implicated as a fluid shear stress sensor that controls pseudopod formation. The role of shear forces on earlier indicators of neutrophil activation, such as L-selectin shedding and α(M)β(2) integrin activation, remains unclear. Here, human neutrophils exposed to uniform shear stress (0.1-4.0 dyn/cm(2)) in a cone-and-plate viscometer for 1-120 min showed a significant reduction in both α(M)β(2) integrin activation and L-selectin shedding after stimulation with 0.5 nM of fMLP. Neutrophil resistance to activation was directly linked to fluid shear stress, as the response increased in a shear stress force- and time-dependent manner. Significant shear

Annexin-1 signals mitogen-stimulated breast tumor cell proliferation by activation of the formyl peptide receptors (FPRs) 1 and...Annexin-1 signals mitogen-stimulated breast tumor cell proliferation by activation of the formyl peptide receptors (FPRs) 1 and...

Annexin A1 (MeSH) * Biochemistry & Molecular Biology (Science Metrix) * Breast Neoplasms (MeSH) * Cell Line, Tumor (MeSH) ... Annexin-1 signals mitogen-stimulated breast tumor cell proliferation by activation of the formyl peptide receptors (FPRs) 1 and ... The role of the calcium- and phospholipid-binding protein annexin I (ANXA1) in cell cycle regulation has been investigated in ...
more infohttps://scholars.latrobe.edu.au/display/publication85414

Annexins sense changes in intracellular pH during hypoxia | Biochemical JournalAnnexins sense changes in intracellular pH during hypoxia | Biochemical Journal

Our results show that annexin A6 and the heterotetramer A2-S100A10 (but not annexins A1, A4 and A5) interact independently of ... We have investigated in vivo the influence of the pHi on the membrane association of human annexins A1, A2, A4, A5 and A6 ... The dimerization of annexin A2 within the annexin A2-S100A10 complex is essential for the pH-dependent membrane interaction at ... We show that hypoxia promotes the interaction of the recombinant annexin A2-S100A10 (p11) and annexin A6 with the plasma ...
more infohttp://www.biochemj.org/content/409/1/65

Annexin A1 - WikipediaAnnexin A1 - Wikipedia

Annexin A1, also known as lipocortin I, is a protein that is encoded by the ANXA1 gene in humans. Annexin A1 belongs to the ... Annexin A1 has also been shown to be associated with treatment resistance. ARID1A loss activates annexin A1 expression, which ... annexin A1 is promptly mobilized to the cell surface and secreted. Annexin A1 promotes neutrophil detachment and apoptosis, and ... anti-inflammatory effects is to increase the synthesis and function of annexin A1. Annexin A1 both suppresses phospholipase A2 ...
more infohttps://en.wikipedia.org/wiki/Annexin_A1

ANXA1 (annexin A1)ANXA1 (annexin A1)

... annexin A1), Authors: Flavia Cristina Rodrigues-Lisoni, Tiago Henrique, Eloiza Helena Tajara. Published in: Atlas Genet ... Similarly to other annexins, annexin A1 is characterized by a C-terminal homologous domain with 4 to 8 repeats of 70-75 ... Loss of annexin A1 expression in breast cancer progression.. Cao Y, Li Y, Edelweiss M, Arun B, Rosen D, Resetkova E, Wu Y, Liu ... Annexin A1 expression and its prognostic significance in human breast cancer.. Wang LP, Bi J, Yao C, Xu XD, Li XX, Wang SM, Li ...
more infohttp://atlasgeneticsoncology.org/Genes/GC_ANXA1.html

ANXA1 (annexin A1)ANXA1 (annexin A1)

... annexin A1), Authors: Flavia Cristina Rodrigues-Lisoni, Tiago Henrique, Eloiza Helena Tajara. Published in: Atlas Genet ... Similarly to other annexins, annexin A1 is characterized by a C-terminal homologous domain with 4 to 8 repeats of 70-75 ... Loss of annexin A1 expression in breast cancer progression.. Cao Y, Li Y, Edelweiss M, Arun B, Rosen D, Resetkova E, Wu Y, Liu ... Annexin A1 expression and its prognostic significance in human breast cancer.. Wang LP, Bi J, Yao C, Xu XD, Li XX, Wang SM, Li ...
more infohttp://atlasgeneticsoncology.org/Genes/ANXA1ID653ch9q21.html

Anti-Annexin A1 antibody (ab97485) | AbcamAnti-Annexin A1 antibody (ab97485) | Abcam

Rabbit polyclonal Annexin A1 antibody validated for WB, IHC, ICC/IF and tested in Human. Immunogen corresponding to recombinant ... Anti-Annexin A1 antibody (ab97485) at 1/1000 dilution + H1299 whole cell lysate at 30 µg. Predicted band size : 39 kDa. ... Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1-198 of Human Annexin A1 ( ... A pair of annexin repeats may form one binding site for calcium and phospholipid. ...
more infohttp://www.abcam.com/annexin-a1-antibody-ab97485.html

Human Annexin A1 peptide (ab47659) | AbcamHuman Annexin A1 peptide (ab47659) | Abcam

Buy our Human Annexin A1 peptide. Ab47659 is a Synthetic peptide for ab46686. Abcam provides free protocols, tips and expert ... I have purchased Annexin A1 peptide (ab47659) and I would like to know if you could recommend me the best storage buffer for ... A pair of annexin repeats may form one binding site for calcium and phospholipid. ...
more infohttps://www.abcam.com/human-annexin-a1-peptide-ab47659.html

ANXA1 - Annexin A1 - Rodentia sp. - ANXA1 gene & proteinANXA1 - Annexin A1 - Rodentia sp. - ANXA1 gene & protein

IPR001464. Annexin. IPR018502. Annexin_repeat. IPR018252. Annexin_repeat_CS. IPR037104. Annexin_sf. IPR002388. ANX1. ... IPR001464. Annexin. IPR018502. Annexin_repeat. IPR018252. Annexin_repeat_CS. IPR037104. Annexin_sf. IPR002388. ANX1. ... sp,P24551,ANXA1_RODSP Annexin A1 OS=Rodentia sp. OX=69158 GN=ANXA1 PE=2 SV=3 ... Annexin 1Add BLAST. 61. ,p>This subsection of the Family and Domains section indicates the positions and types of repeated ...
more infohttp://www.uniprot.org/uniprot/P24551

Neonatal Hyperoxic Exposure Persistently Alters Lung Secretoglobins and Annexin A1Neonatal Hyperoxic Exposure Persistently Alters Lung Secretoglobins and Annexin A1

... Thomas M. Raffay,1 Morgan L. Locy,2 Cynthia ... Expression of lung SCGB and annexin A1 (ANXA1) is persistently altered in CCSP knockout mice suggesting that CCSP indirectly ...
more infohttps://www.hindawi.com/journals/bmri/2013/408485/abs/

Mono-Ubiquitination of Nuclear Annexin A1 and Mutagenesis | IntechOpenMono-Ubiquitination of Nuclear Annexin A1 and Mutagenesis | IntechOpen

Mono-Ubiquitination of Nuclear Annexin A1 and Mutagenesis , IntechOpen, Published on: 2012-08-17. Authors: Fusao Hirata, George ... Native annexin A1 was detected at pI 6.4. The reaction mixture containing SUMOylated annexin A1 showed a new annexin A1 ... when amounts of ubiquitinated annexin A1 were adjusted with the total amounts of annexin A1 (free and ubiquitinated annexin A1 ... 6. Heavy metals and annexin A1. Annexin A1 has 3 different types of Ca2+ binding sites, type II, type III and type III (AB) ( ...
more infohttps://www.intechopen.com/books/mutagenesis/mono-ubiquitination-of-nuclear-annexin-a1-and-mutagenesis/

ANXA1/Annexin A1 Antibody | Bethyl Laboratories, Inc.ANXA1/Annexin A1 Antibody | Bethyl Laboratories, Inc.

Unconjugated Whole IgG Rabbit anti-ANXA1/Annexin A1 Antibody, Affinity Purified suitable for WB, IP, IHC applications. Visit ... ANXA1/Annexin A1 Antibody, A305-235A. ANXA1/Annexin A1 Antibody, A305-235A. ...
more infohttps://www.bethyl.com/product/A305-235A?referrer=Signal+Transduction&target=ANXA1+Annexin+A1

ANXA1 (annexin A1) - KOMP (Knockout Mouse Project)ANXA1 (annexin A1) - KOMP (Knockout Mouse Project)

annexin A1. Synonyms: Anx-1, Anx-A1, C430014K04Rik, Lpc-1, Lpc1. Gene nomenclature, locus information, and GO, OMIM, and PMID ... OMIM: ANNEXIN A1; ANXA1*Gene Ontology: Anxa1 *Mouse Phenome DB: Anxa1 *UCSC: Chr.19:20,373,434-20,390,671(-)*IMPC: Anxa1 ...
more infohttps://www.komp.org/geneinfo.php?geneid=23383

Annexin A1 and the Resolution of Inflammation: Modulation of Neutrophil Recruitment, Apoptosis, and ClearanceAnnexin A1 and the Resolution of Inflammation: Modulation of Neutrophil Recruitment, Apoptosis, and Clearance

... Michelle Amantéa ... Annexin A1 (AnxA1), also known as lipocortin-1, is an endogenous glucocorticoid-regulated protein, which is able to ...
more infohttps://www.hindawi.com/journals/jir/2016/8239258/abs/

Annexin A1 Counteracts Chemokine-Induced Arterial Myeloid Cell RecruitmentNovelty and Significance | Circulation ResearchAnnexin A1 Counteracts Chemokine-Induced Arterial Myeloid Cell RecruitmentNovelty and Significance | Circulation Research

Annexin A1 counteracts chemokine-induced integrin activation. A and B, Annexin A1 inhibits chemokine-evoked integrin activation ... In vivo delivery of the annexin A1 fragment Ac2-26 reduces atherosclerosis. A-C, Apoe−/−, Apoe−/−Fpr2−/−, and Apoe−/−Anxa1−/− ... Annexin A1-formyl peptide receptor 2 axis prevents arterial myeloid cell recruitment. Apoe−/−, Apoe−/−Fpr2−/−, and Apoe−/−Anxa1 ... Formyl peptide receptor 2 and its ligand annexin A1 protect from atherogenesis. Apoe−/−, Apoe−/−Fpr2−/−, and Apoe−/−Anxa1−/− ...
more infohttp://circres.ahajournals.org/content/116/5/827

JCM | Free Full-Text | Annexin A1 as Neuroprotective Determinant for Blood-Brain Barrier Integrity in Neonatal Hypoxic-Ischemic...JCM | Free Full-Text | Annexin A1 as Neuroprotective Determinant for Blood-Brain Barrier Integrity in Neonatal Hypoxic-Ischemic...

We hypothesized that Annexin A1 (ANXA1), present in MSC-EVs, contributed to their therapeutic potential by targeting the ANXA1/ ... Annexin A1 as Neuroprotective Determinant for Blood-Brain Barrier Integrity in Neonatal Hypoxic-Ischemic Encephalopathy Ruth ... We hypothesized that Annexin A1 (ANXA1), present in MSC-EVs, contributed to their therapeutic potential by targeting the ANXA1/ ... Keywords: Annexin A1/Formyl peptide receptor axis; blood-brain barrier; mesenchymal stem cell-derived extracellular vesicles; ...
more infohttps://www.mdpi.com/2077-0383/8/2/137

Cleavage of Annexin A1 by ADAM10 during Secondary Necrosis Generates a Monocytic Find-Me Signal | The Journal of ImmunologyCleavage of Annexin A1 by ADAM10 during Secondary Necrosis Generates a Monocytic "Find-Me" Signal | The Journal of Immunology

... annexin A1-externalizing cells induced chemoattraction of monocytes, which was clearly reduced in annexin A1- or ADAM10- ... Cleavage of Annexin A1 by ADAM10 during Secondary Necrosis Generates a Monocytic "Find-Me" Signal. Karin E. Blume, Szabolcs ... Annexin A1 is an intracellular calcium/phospholipid-binding protein that is involved in membrane organization and the ... As such, annexin A1 promotes the engulfment of dying cells and dampens the postphagocytic production of proinflammatory ...
more infohttp://www.jimmunol.org/content/early/2011/11/23/jimmunol.1004073

An annexin A1-FPR1 interaction contributes to necroptosis of keratinocytes in severe cutaneous adverse drug reactions | Science...An annexin A1-FPR1 interaction contributes to necroptosis of keratinocytes in severe cutaneous adverse drug reactions | Science...

Mass spectrometric analysis identified annexin A1 as a key mediator of keratinocyte death; depletion of annexin A1 by a ... An annexin A1-FPR1 interaction contributes to necroptosis of keratinocytes in severe cutaneous adverse drug reactions ... Annexin A1 secreted from drug-stimulated monocytes contributes to keratinocyte necroptosis in serious drug-related adverse ... Annexin A1 secreted from drug-stimulated monocytes contributes to keratinocyte necroptosis in serious drug-related adverse ...
more infohttp://stm.sciencemag.org/content/6/245/245ra95

Pro- and anti-inflammatory actions in coronary artery disease : with focus on CD56+ T cells and Annexin A1Pro- and anti-inflammatory actions in coronary artery disease : with focus on CD56+ T cells and Annexin A1

Open this publication in new window or tab ,,Higher expression of annexin A1 in 1 CD56+ than in CD56-T cells: Potential ... Pro- and anti-inflammatory actions in coronary artery disease: with focus on CD56+ T cells and Annexin A1. Bergström, Ida ... Annexin A1 (AnxA1) is a glucocorticoidinduced protein which may have a key role in the anti-inflammatory response as a mediator ... 3. Higher expression of annexin A1 in 1 CD56+ than in CD56-T cells: Potential implications for coronary artery disease. ...
more infohttp://liu.diva-portal.org/smash/record.jsf?pid=diva2:787338

ANXA1 recombinant protein | Annexin A1 (ANXA1) Recombinant Protein-NP 001075336.1ANXA1 recombinant protein | Annexin A1 (ANXA1) Recombinant Protein-NP 001075336.1

Annexin A1 (ANXA1) Recombinant Protein-NP_001075336.1 (MBS1346876) product datasheet at MyBioSource, Recombinant Proteins ... Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately ... Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since ... annexin I may have potential anti-inflammatory activity. ... ANXA1 recombinant protein :: Annexin A1 (ANXA1) Recombinant ...
more infohttps://www.mybiosource.com/anxa1-recombinant-protein/annexin-a1-anxa1/1346876

Reperfusion-induced Myocardial Dysfunction is Prevented by Endogenous Annexin-A1 and Its N-terminal Derived Peptide Ac-ANX-A1 ...Reperfusion-induced Myocardial Dysfunction is Prevented by Endogenous Annexin-A1 and Its N-terminal Derived Peptide Ac-ANX-A1 ...

Reperfusion-induced Myocardial Dysfunction is Prevented by Endogenous Annexin-A1 and Its N-terminal Derived Peptide Ac-ANX-A1 ( ... Reperfusion-induced Myocardial Dysfunction is Prevented by Endogenous Annexin-A1 and Its N-terminal Derived Peptide Ac-ANX-A1 ( ...
more infohttps://www.mcri.edu.au/publications/reperfusion-induced-myocardial-dysfunction-prevented-endogenous-annexin-a1-and-its-n

Annexin A1 Deficiency does not Affect Myofiber Repair but Delays Regen by Evgenia Leikina, Aurelia Defour et al."Annexin A1 Deficiency does not Affect Myofiber Repair but Delays Regen" by Evgenia Leikina, Aurelia Defour et al.

In vitro experiments have identified involvement of Annexin A1 (Anx A1) in both these fusion processes. To determine if Anx A1 ... Instead, lack of Anx A1 lowered the proportion of differentiating myoblasts that managed to fuse with the injured myofibers by ... Despite this early slowdown in fusion of Anx A1-/- myoblasts, regeneration caught up at later times post injury. These results ... However, the lack of Anx A1 delayed muscle regeneration after notexin-induced injury. This delay in muscle regeneration was not ...
more infohttps://hsrc.himmelfarb.gwu.edu/smhs_intsysbio_facpubs/164/

Modulation of experimental autoimmune encephalomyelitis by endogenous Annexin A1 | Journal of Neuroinflammation | Full TextModulation of experimental autoimmune encephalomyelitis by endogenous Annexin A1 | Journal of Neuroinflammation | Full Text

Analysis of MOG35-55-induced EAE development in Annexin A1 null mice showed decreased signs of the disease compared to wild ... We have reported novel biological functions for Annexin A1, an effector of endogenous anti-inflammation, to produce positive ... In this study, we investigated the potential modulatory role of Annexin A1 in the development of experimental autoimmune ... Together these findings suggest that Annexin A1 null mice have an impaired capacity to develop EAE. Furthermore strategies ...
more infohttps://jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-6-33

KSE787Ra01 | ELISA Kit DIY Materials for Annexin A1 (ANXA1) | Rattus norvegicus (Rat) USCN(Wuhan USCN Business Co., Ltd. )KSE787Ra01 | ELISA Kit DIY Materials for Annexin A1 (ANXA1) | Rattus norvegicus (Rat) USCN(Wuhan USCN Business Co., Ltd. )

ANX-A1; ANX1; LPC1; Lipocortin I; Chromobindin-9; Calpactin II; Phospholipase A2 inhibitory protein , Products for research use ... ELISA Kit DIY Materials for Annexin A1 (ANXA1), ... ELISA Kit DIY Materials for Annexin A1 (ANXA1). ANX-A1; ANX1; ... Monoclonal Antibody to Annexin A1 (ANXA1). WB; IHC; ICC; IP.. SEE787Ra. ELISA Kit for Annexin A1 (ANXA1). Enzyme-linked ... Polyclonal Antibody to Annexin A1 (ANXA1). WB; IHC; ICC; IP.. LAE787Ra71. Biotin-Linked Polyclonal Antibody to Annexin A1 ( ...
more infohttp://uscnk.com/uscn/ELISA-Kit-DIY-Materials-for-Annexin-A1-

Decreased expression of annexin A1 is correlated with breast cancer development and progression as determined by a tissue...Decreased expression of annexin A1 is correlated with breast cancer development and progression as determined by a tissue...

You are here: Home / Publications / Decreased expression of annexin A1 is correlated with breast cancer development and ... Decreased expression of annexin A1 is correlated with breast cancer development and progression as determined by a tissue ... Annexin A1 (ANXA1) is a calcium- and phospholipid-binding protein and a known mediator of glucocorticoid-regulated inflammatory ...
more infohttps://edrn.nci.nih.gov/publications/16949910-decreased-expression-of-annexin-a1-is

Up-regulation of Annexin-A1 and lipoxin A(4) in individuals with ulcerative colitis may promote mucosal homeostasis.Up-regulation of Annexin-A1 and lipoxin A(4) in individuals with ulcerative colitis may promote mucosal homeostasis.

... * QMRO ... Up-regulation of Annexin-A1 and lipoxin A(4) in individuals with ulcerative colitis may promote mucosal homeostasis.. ... Up-regulation of Annexin-A1 and lipoxin A(4) in individuals with ulcerative colitis may promote mucosal homeostasis. ... The pro-resolution mediators Annexin-A1 (AnxA1) and lipoxin A(4) (LXA(4)) exert counter-regulatory effects on leukocyte ...
more infohttps://qmro.qmul.ac.uk/xmlui/handle/123456789/5320
  • I have purchased Annexin A1 peptide (ab47659) and I would like to know if you could recommend me the best storage buffer for this product. (abcam.com)
  • Most importantly, we demonstrate that the released peptide and culture supernatants of secondary necrotic, annexin A1-externalizing cells induced chemoattraction of monocytes, which was clearly reduced in annexin A1- or ADAM10-knockdown cells. (jimmunol.org)
  • Thus, altogether our findings indicate that annexin A1 externalization and its proteolytic processing into a chemotactic peptide represent final events during apoptosis, which after the transition to secondary necrosis contribute to the recruitment of monocytes and the prevention of inflammation. (jimmunol.org)
  • The necroptosis-mediating complex of RIP1 and RIP3 was indispensable for SJS/TEN supernatant-induced keratinocyte death, and SJS/TEN keratinocytes expressed abundant formyl peptide receptor 1 (FPR1), the receptor for annexin A1, whereas control keratinocytes did not. (sciencemag.org)
  • Annexin A1 promotes neutrophil detachment and apoptosis, and phagocytosis of apoptotic neutrophils by macrophages. (wikipedia.org)
  • Together these findings suggest that Annexin A1 null mice have an impaired capacity to develop EAE. (biomedcentral.com)
  • Amino acids M1 - N346(end) of human Annexin A1. (dundee.ac.uk)
  • Our results demonstrate that a necroptosis pathway, likely mediated by annexin 1 acting through the FPR1 receptor, contributes to SJS/TEN. (sciencemag.org)
  • Instead, lack of Anx A1 lowered the proportion of differentiating myoblasts that managed to fuse with the injured myofibers by days 5 and 7 after notexin injury as compared to the wild type (w.t.) mice. (gwu.edu)
  • Analysis of MOG 35-55 -induced EAE development in Annexin A1 null mice showed decreased signs of the disease compared to wild type mice. (biomedcentral.com)
  • These results establish in vivo role of Anx A1 in cell fusion required for myofiber regeneration and not in intracellular vesicle fusion needed for repair of myofiber sarcolemma. (gwu.edu)
  • 2010). Therefore, nuclear annexin A1 is thought to play an important role in cell proliferation and/or cell transformation. (intechopen.com)
  • it is likely that nuclear annexin A1 is involved in DNA replication, especially DNA damage induced gene mutation, since DNA damage induced mutagenesis plays an important role in tumorigenesis. (intechopen.com)
  • In this study, we investigated the potential modulatory role of Annexin A1 in the development of experimental autoimmune encephalomyelitis, a model of multiple sclerosis. (biomedcentral.com)
  • Finally, analysis of the T cell recall response in vitro following stimulation with MOG 35-55 showed a decrease proliferation of Annexin A1 null T cells, with a significantly reduced Th1/Th17 phenotype, compared to wild type cells. (biomedcentral.com)
  • Following cell activation (for example, by neutrophil adhesion to endothelial-cell monolayers), annexin A1 is promptly mobilized to the cell surface and secreted. (wikipedia.org)
  • In our current follow-up study, we report that exposure of annexin A1 during secondary necrosis coincided with proteolytic processing within its unique N-terminal domain by ADAM10. (jimmunol.org)
  • Annexin A1 has been of interest for use as a potential anticancer drug. (wikipedia.org)