A class of organic compounds that contains a naphthalene moiety linked to a sulfonic acid salt or ester.
A class of organic compounds which contain an anilino (phenylamino) group linked to a salt or ester of naphthalenesulfonic acid. They are frequently used as fluorescent dyes and sulfhydryl reagents.
An enzyme that catalyzes the transfer of the planetary sulfur atom of thiosulfate ion to cyanide ion to form thiocyanate ion. EC 2.8.1.1.
Measurement of the intensity and quality of fluorescence.
Disruption of the non-covalent bonds and/or disulfide bonds responsible for maintaining the three-dimensional shape and activity of the native protein.
Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
The specialty of ANALYTIC CHEMISTRY applied to assays of physiologically important substances found in blood, urine, tissues, and other biological fluids for the purpose of aiding the physician in making a diagnosis or following therapy.
The interchange of goods or commodities, especially on a large scale, between different countries or between populations within the same country. It includes trade (the buying, selling, or exchanging of commodities, whether wholesale or retail) and business (the purchase and sale of goods to make a profit). (From Random House Unabridged Dictionary, 2d ed, p411, p2005 & p283)
Laboratory tests demonstrating the presence of physiologically significant substances in the blood, urine, tissue, and body fluids with application to the diagnosis or therapy of disease.
Governmental levies on property, inheritance, gifts, etc.
Health care professionals, technicians, and assistants staffing LABORATORIES in research or health care facilities.
A subspecialty of pathology applied to the solution of clinical problems, especially the use of laboratory methods in clinical diagnosis. (Dorland, 28th ed.)
Techniques used to carry out clinical investigative procedures in the diagnosis and therapy of disease.
Globulins of milk obtained from the WHEY.
A fibrous protein complex that consists of proteins folded into a specific cross beta-pleated sheet structure. This fibrillar structure has been found as an alternative folding pattern for a variety of functional proteins. Deposits of amyloid in the form of AMYLOID PLAQUES are associated with a variety of degenerative diseases. The amyloid structure has also been found in a number of functional proteins that are unrelated to disease.
The assembly of the QUATERNARY PROTEIN STRUCTURE of multimeric proteins (MULTIPROTEIN COMPLEXES) from their composite PROTEIN SUBUNITS.
A synuclein that is a major component of LEWY BODIES that plays a role in neurodegeneration and neuroprotection.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The vital life force in the body, supposedly able to be regulated by acupuncture. It corresponds roughly to the Greek pneuma, the Latin spiritus, and the ancient Indian prana. The concept of life-breath or vital energy was formulated as an indication of the awareness of man, originally directed externally toward nature or society but later turned inward to the self or life within. (From Comparison between Concepts of Life-Breath in East and West, 15th International Symposium on the Comparative History of Medicine - East and West, August 26-September 3, 1990, Shizuoka, Japan, pp. ix-x)
Substances that are energetically unstable and can produce a sudden expansion of the material, called an explosion, which is accompanied by heat, pressure and noise. Other things which have been described as explosive that are not included here are explosive action of laser heating, human performance, sudden epidemiological outbreaks, or fast cell growth.
Four or five slender jointed digits in humans and primates, attached to each HAND.
The volume of packed RED BLOOD CELLS in a blood specimen. The volume is measured by centrifugation in a tube with graduated markings, or with automated blood cell counters. It is an indicator of erythrocyte status in disease. For example, ANEMIA shows a low value; POLYCYTHEMIA, a high value.
Exclusive legal rights or privileges applied to inventions, plants, etc.
The minute vessels that connect the arterioles and venules.
Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)
The number of units (persons, animals, patients, specified circumstances, etc.) in a population to be studied. The sample size should be big enough to have a high likelihood of detecting a true difference between two groups. (From Wassertheil-Smoller, Biostatistics and Epidemiology, 1990, p95)
A plant family of the order Theales.
A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere.
Very toxic industrial chemicals. They are absorbed through the skin, causing lethal blood, bladder, liver, and kidney damage and are potent, broad-spectrum carcinogens in most species.
Narrow pieces of material impregnated or covered with a substance used to produce a chemical reaction. The strips are used in detecting, measuring, producing, etc., other substances. (From Dorland, 28th ed)
Methods utilizing the principles of MICROFLUIDICS for sample handling, reagent mixing, and separation and detection of specific components in fluids.
The temperature at which a substance changes from one state or conformation of matter to another.
The study of fluid channels and chambers of tiny dimensions of tens to hundreds of micrometers and volumes of nanoliters or picoliters. This is of interest in biological MICROCIRCULATION and used in MICROCHEMISTRY and INVESTIGATIVE TECHNIQUES.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
A novel composition, device, or process, independently conceived de novo or derived from a pre-existing model.
Property, such as patents, trademarks, and copyright, that results from creative effort. The Patent and Copyright Clause (Art. 1, Sec. 8, cl. 8) of the United States Constitution provides for promoting the progress of science and useful arts by securing for limited times to authors and inventors, the exclusive right to their respective writings and discoveries. (From Black's Law Dictionary, 5th ed, p1014)
Layers of lipid molecules which are two molecules thick. Bilayer systems are frequently studied as models of biological membranes.
Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.
Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a choline moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and choline and 2 moles of fatty acids.
Artificial, single or multilaminar vesicles (made from lecithins or other lipids) that are used for the delivery of a variety of biological molecules or molecular complexes to cells, for example, drug delivery and gene transfer. They are also used to study membranes and membrane proteins.
A generic term for fats and lipoids, the alcohol-ether-soluble constituents of protoplasm, which are insoluble in water. They comprise the fats, fatty oils, essential oils, waxes, phospholipids, glycolipids, sulfolipids, aminolipids, chromolipids (lipochromes), and fatty acids. (Grant & Hackh's Chemical Dictionary, 5th ed)
Artificially produced membranes, such as semipermeable membranes used in artificial kidney dialysis (RENAL DIALYSIS), monomolecular and bimolecular membranes used as models to simulate biological CELL MEMBRANES. These membranes are also used in the process of GUIDED TISSUE REGENERATION.
Cytoplasm stored in an egg that contains nutritional reserves for the developing embryo. It is rich in polysaccharides, lipids, and proteins.
A low-energy attractive force between hydrogen and another element. It plays a major role in determining the properties of water, proteins, and other compounds.
Lipids, predominantly phospholipids, cholesterol and small amounts of glycolipids found in membranes including cellular and intracellular membranes. These lipids may be arranged in bilayers in the membranes with integral proteins between the layers and peripheral proteins attached to the outside. Membrane lipids are required for active transport, several enzymatic activities and membrane formation.
The characteristic three-dimensional shape of a molecule.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
The technology of transmitting light over long distances through strands of glass or other transparent material.
Thin strands of transparent material, usually glass, that are used for transmitting light waves over long distances.
Failure in built environment with loss of functional integrity.
The transfer of energy of a given form among different scales of motion. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed). It includes the transfer of kinetic energy and the transfer of chemical energy. The transfer of chemical energy from one molecule to another depends on proximity of molecules so it is often used as in techniques to measure distance such as the use of FORSTER RESONANCE ENERGY TRANSFER.
A type of FLUORESCENCE SPECTROSCOPY using two FLUORESCENT DYES with overlapping emission and absorption spectra, which is used to indicate proximity of labeled molecules. This technique is useful for studying interactions of molecules and PROTEIN FOLDING.

Opposite behavior of two isozymes when refolding in the presence of non-ionic detergents. (1/531)

GroEL has a greater affinity for the mitochondrial isozyme (mAAT) of aspartate aminotransferase than for its cytosolic counterpart (cAAT) (Mattingly JR Jr, Iriarte A, Martinez-Carrion M, 1995, J Biol Chem 270:1138-1148), two proteins that share a high degree of sequence similarity and an almost identical spatial structure. The effect of detergents on the refolding of these large, dimeric isozymes parallels this difference in behavior. The presence of non-ionic detergents such as Triton X-100 or lubrol at concentrations above their critical micelle concentration (CMC) interferes with reactivation of mAAT unfolded in guanidinium chloride but increases the yield of cAAT refolding at low temperatures. The inhibitory effect of detergents on the reactivation of mAAT decreases progressively as the addition of detergents is delayed after starting the refolding reaction. The rate of disappearance of the species with affinity for binding detergents coincides with the slowest of the two rate-limiting steps detected in the refolding pathway of mAAT. Limited proteolysis studies indicate that the overall structure of the detergent-bound mAAT resembles that of the protein in a complex with GroEL. The mAAT folding intermediates trapped in the presence of detergents can resume reactivation either upon dilution of the detergent below its CMC or by adding beta-cyclodextrin. Thus, isolation of otherwise transient productive folding intermediates for further characterization is possible through the use of detergents.  (+info)

Effect of the hemolytic lectin CEL-III from Holothuroidea Cucumaria echinata on the ANS fluorescence responses in sensitive MDCK and resistant CHO cells. (2/531)

The addition of CEL-III to sensitive MDCK cells preincubated with 8-anilino-1-naphthalenesulfonate (ANS) caused an increase in the fluorescence intensity of the probe. The increase in the ANS fluorescence caused by CEL-III was Ca2+-dependent and strongly inhibited by 0.1 M lactose, indicating that Ca2+-dependent binding of CEL-III to specific carbohydrate receptors on the plasma membrane is responsible for this phenomenon. In contrast, no significant effect of CEL-III on the ANS fluorescence was observed in CHO cells, which are highly resistant to CEL-III cytotoxicity. In MDCK cells, energy transfer from tryptophan residues to bound ANS molecules was observed in the presence of CEL-III, but not in CHO cells. Furthermore, the amount of ANS bound to MDCK cells increased as the concentration of CEL-III increased. Therefore, a simple interpretation is that the CEL-III-induced increase in ANS fluorescence is attributable to an increase of the hydrophobic region in the plasma membrane where ANS could bind. Immunoblotting analysis of proteins from cells treated with CEL-III indicated that CEL-III oligomers were irreversibly bound to the cells, and the amount of oligomer bound to MDCK cells was much greater than that bound to CHO cells under any conditions tested. The oligomerization may be accompanied by an enhancement of the hydrophobicity of CEL-III molecules, which in turn provides new ANS-binding sites. The difference in susceptibility of MDCK and CHO cells to CEL-III cytotoxicity may be due to a difference in oligomerization of bound CEL-III.  (+info)

The Mycobacterium tuberculosis small heat shock protein Hsp16.3 exposes hydrophobic surfaces at mild conditions: conformational flexibility and molecular chaperone activity. (3/531)

Hsp16.3, the alpha-crystallin-related small heat shock protein of Mycobacterium tuberculosis that is maximally expressed during the stationary phase and is a major membrane protein, has been reported to form specific trimer-of-trimers structure and to act as an effective molecular chaperone (Chang Z et al., 1996, J. Biol Chem 271:7218-7223). However, little is known about its action mechanism. In this study, Hsp16.3 conformational intermediates with dramatically increased chaperone activities were detected after treatment with very low concentrations of guanidine hydrochloride (0.05 M), urea (0.3 M), or mild heating (30 degrees C). The intermediates showed a significant increase in their capacity to bind the hydrophobic probe 1-anilino-8-naphthalene sulfonate (ANS), indicating an increased exposure of hydrophobic surfaces. Interestingly, the greatest chaperone activities of Hsp16.3 were observed in the presence of 0.3 M guanidine HCl or when heated to 35 degrees C. CD spectroscopy studies revealed no significant changes in protein secondary and tertiary structures at these mild treatments. Our in vitro studies also indicate that long-time-heated Hsp16.3, heated even to temperatures as high as 85 degrees C, has almost the same, if not a slightly greater, chaperone activities as the native protein when cooled to room temperature and its secondary structures also almost recovered. Together, these results suggest that Hsp16.3 modulates its chaperone activity by exposing hydrophobic surfaces and that the protein structure is highly stable and flexible, thus highly adapted for its function.  (+info)

Fluorescence measurements detect changes in scallop myosin regulatory domain. (4/531)

Ca2+-induced conformational changes of scallop myosin regulatory domain (RD) were studied using intrinsic fluorescence. Both the intensity and anisotropy of tryptophan fluorescence decreased significantly upon removal of Ca2+. By making a mutant RD we found that the Ca2+-induced fluorescence change is due mainly to Trp21 of the essential light chain which is located at the unusual Ca2+-binding EF-hand motif of the first domain. This result suggests that Trp21 is in a less hydrophobic and more flexible environment in the Ca2+-free state, supporting a model for regulation based on the 2 A resolution structure of scallop RD with bound Ca2+ [Houdusse A. and Cohen C. (1996) Structure 4, 21-32]. Binding of the fluorescent probe, 8-anilinonaphthalene-1-sulphonate (ANS) to the RD senses the dissociation of the regulatory light chain (RLC) in the presence of EDTA, by energy transfer from a tryptophan cluster (Trp818, 824, 826, 827) on the heavy chain (HC). We identified a hydrophobic pentapeptide (Leu836-Ala840) at the head-rod junction which is required for the effective energy transfer and conceivably is part of the ANS-binding site. Extension of the HC component of RD towards the rod region results in a larger ANS response, presumably indicating changes in HC-RLC interactions, which might be crucial for the regulatory function of scallop myosin.  (+info)

Conformational intermediates and fusion activity of influenza virus hemagglutinin. (5/531)

Three strains of influenza virus (H1, H2, and H3) exhibited similar characteristics in the ability of their hemagglutinin (HA) to induce membrane fusion, but the HAs differed in their susceptibility to inactivation. The extent of inactivation depended on the pH of preincubation and was lowest for A/Japan (H2 subtype), in agreement with previous studies (A. Puri, F. Booy, R. W. Doms, J. M. White, and R. Blumenthal, J. Virol. 64:3824-3832, 1990). While significant inactivation of X31 (H3 subtype) was observed at 37 degrees C at pH values corresponding to the maximum of fusion (about pH 5.0), no inactivation was seen at preincubation pH values 0.2 to 0.4 pH units higher. Surprisingly, low-pH preincubation under those conditions enhanced the fusion rates and extents of A/Japan as well as those of X31. For A/PR 8/34 (H1 subtype), neither a shift of the pH (to >5.0) nor a decrease of the temperature to 20 degrees C was sufficient to prevent inactivation. We provide evidence that the activated HA is a conformational intermediate distinct from the native structure and from the final structure associated with the conformational change of HA, which is implicated by the high-resolution structure of the soluble trimeric fragment TBHA2 (P. A. Bullough, F. M. Hughson, J. J. Skehel, and D. C. Wiley, Nature 371:37-43, 1994).  (+info)

Role of regulatory exosite I in binding of thrombin to human factor V, factor Va, factor Va subunits, and activation fragments. (6/531)

The blood coagulation proteinase, thrombin, converts factor V into factor Va through a multistep activation pathway that is regulated by interactions with thrombin exosites. Thrombin exosite interactions with human factor V and its activation products were quantitatively characterized in equilibrium binding studies based on fluorescence changes of thrombin covalently labeled with 2-anilinonaphthalene-6-sulfonic acid (ANS) linked to the catalytic site histidine residue by Nalpha-[(acetylthio)acetyl]-D-Phe-Pro-Arg-CH2Cl ([ANS]FPR-thrombin). Exosite I was shown to play a predominant role in the binding of factor V and factor Va from the effect of the exosite I-specific ligand, hirudin54-65, on the interactions. Factor V and factor Va bound to exosite I of [ANS]FPR-thrombin with similar dissociation constants of 3.4 +/- 1.3 and 1.1 +/- 0.4 microM and fluorescence enhancements of 182 +/- 41 and 127 +/- 17%, respectively. Native thrombin and labeled thrombin bound with similar affinity to factor Va. Among factor V activation products, the factor Va heavy chain was shown to contain the site of exosite I binding, whereas exosite I-independent, lower affinity interactions were observed for activation fragments E and C1, and no detectable binding was observed for the factor Va light chain. The results support the conclusion that the factor V activation pathway is initiated by exosite I-mediated binding of thrombin to a site in the heavy chain region of factor V that facilitates the initial cleavage at Arg709 to generate the heavy chain of factor Va. The results further suggest that binding of thrombin through exosite I to factor V activation intermediates may regulate their conversion to factor Va and that similar binding of thrombin to the factor Va produced may reflect a mode of interaction involved in the regulation of prothrombin activation.  (+info)

The membrane topology of proton-pumping Escherichia coli transhydrogenase determined by cysteine labeling. (7/531)

The membrane topology of proton-pumping nicotinamide-nucleotide transhydrogenase from Escherichia coli was determined by site-specific chemical labeling. A His-tagged cysteine-free transhydrogenase was used to introduce unique cysteines in positions corresponding to potential membrane loops. The cysteines were reacted with fluorescent reagents, fluorescein 5-maleimide or 2-[(4'-maleimidyl)anilino]naphthalene-6-sulfonic acid, in both intact cells and inside-out vesicles. Labeled transhydrogenase was purified with a small-scale procedure using a metal affinity resin, and the amount of labeling was measured as fluorescence on UV-illuminated acrylamide gels. The difference in labeling between intact cells and inside-out vesicles was used to discriminate between a periplasmic and a cytosolic location of the residues. The membrane region was found to be composed of 13 helices (four in the alpha-subunit and nine in the beta-subunit), with the C terminus of the alpha-subunit and the N terminus of the beta-subunit facing the cytosolic and periplasmic sides, respectively. These results differ from previous models with regard to both number of helices and the relative location and orientation of certain helices. This study constitutes the first in which all transmembrane segments of transhydrogenase have been experimentally determined and provides an explanation for the different topologies of the mitochondrial and E. coli transhydrogenases.  (+info)

Evidence of heterogeneous 1-anilinonaphthalene-8-sulfonate binding to beta-lactoglobulin from fluorescence spectroscopy. (8/531)

Steady-state and dynamic fluorescence titrations show that: (a) the complex between beta-lactoglobulin (BLG) and 1-anilinonaphthalene-8-sulfonate (ANS) displays a heterogeneous equilibrium with large changes in the binding strength vs. pH and ion concentration; and (b) the fluorescence response of bound ANS reveals two separate lifetimes that suggest two different sites (or binding modes). While steady-state fluorescence titrations yield effective values of the binding constant and of the bound ANS quantum efficiency, it is shown that, by combining steady-state fluorescence and lifetime decay of ANS, it is possible to give quantitative estimates of the association constants for each site. When heading from the acid (pH approximately 2) to the native state (pH approximately 6) the main result is a very large reduction of the effective binding constant. This and the results of titrations vs. ionic strength suggest that electrostatic interactions are a major contribution to ANS binding to BLG.  (+info)

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PubMed. Faber, E. B.; Tian, D.; Burban, D.; Levinson, N. M.; Hawkinson, J. E.; Georg, G. I. Cooperativity Between Orthosteric Inhibitors and Allosteric Inhibitor 8-Anilino-1-Naphthalene Sulfonic Acid(ANS) in Cyclin-dependent Kinase 2. ACS Chem. Biol. 2020, 15, 1759-1764.. Holth, T. A. D.; Walters, M.; Hutt, O. E.; Georg, G. I. Diversity-Oriented Library Synthesis from Steviol and Isosteviol-Derived Scaffolds. ACS Comb. Sci. 2020, 22, 150-155. DOI: 10.1021/acscombsci.9b00186.. Wang, N.; Faber, E. B.; Georg, G. I. Synthesis and Spectral Properties of 8-Anilinonaphthalene-1- Sulfonic Acid (ANS) Derivatives Prepared by Microwave-Assisted Copper(0)-Catalyzed UllmanReaction. ACS Omega 2019, 4, 18472-18477.. ? Syeda, S. S.; Sánchez, G.; Hong, K. H.; Hawkinson, J.; Georg, G. I.; Blanco, G. Design, Synthesis, In Vitro and In Vivo Evaluation of Ouabain Analogs as Potent and Selective Na,K- ATPase a4 Isoform Inhibitors for Male Contraception.J. Med. Chem. 2018, 61, 1800-1820. ? Paulson,C. N.; Guan,X.; ...
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TY - GEN. T1 - 2 - NSA, 1,5 - NDSA application and sodium naphtionate as fluorescent indicators at oil field. AU - Bilyalov, Alisher A.. AU - Gavrilenko, Mikhail A.. AU - Gavrilenko, Nataliya Airatovna. PY - 2014. Y1 - 2014. N2 - Set of polyaromatic sulfonates for tracer analysis of oil field as a result of fluorescent spectra study is recommended. Simultaneous determination of fluorescent polyaromatic sulfonates is confirmed by the applicability and reproducibility of the fluorescent method.. AB - Set of polyaromatic sulfonates for tracer analysis of oil field as a result of fluorescent spectra study is recommended. Simultaneous determination of fluorescent polyaromatic sulfonates is confirmed by the applicability and reproducibility of the fluorescent method.. KW - Fluorescent analysis. KW - Fluorescent dyes. KW - Indicators. KW - Naphthalene sulfonic acids. UR - http://www.scopus.com/inward/record.url?scp=84913595819&partnerID=8YFLogxK. UR - ...
(1R,2S)-2-amino-1-phenyl-propan-1-ol; 4-benzhydryloxy-1-methyl-piperidine; (3-carbamoyl-3,3-diphenyl-propyl)-methyl-dipropan-2-yl-azanium; iodide; dihydrochloride 4,6-dichloro-3H-benzothiazole-2-thione 5,12-Naphthacenedione, 10-[(3-amino-2,3, 6-trideoxy-.alpha.-L-lyxo-hexopyranosyl)oxy]-7,8,9, 10-tetrahydro-6,8,11-trihydroxy-8-(hydroxyacetyl)-1,7-dimethoxy-, hydrochloride, [7R-(7.alpha.,8.beta.,10.beta.)]- (E)-N-cinnamyl-N-methyl-3-phenyl-prop-2-en-1-amine 2-[[amino-(3,4-dimethoxyphenyl)methylidene]amino]ethanesulfonic acid 1-(2-hydroxyethyl)-3-(4-methoxyphenyl)-1-methyl-urea 2-chloro-1-phenyl-indole-3-carbaldehyde Rubber, cyclized Chromium, 2-hydroxy-3,5-dinitrobenzenediazonium-4(or 5)-hydroxy-1-naphthalenesulfonic acid coupling products 3-hydroxy-4-((1-hydroxy-2-naphthalenyl)azo)-1-naphthalenesulfonate complexes, compds. with N,N-bis(Ph and o-tolyl and xylyl)guanidine 10-(4-dimethylamino-5-hydroxy-6-methyl-oxan-2-yl)oxy-8-ethyl-6,7,8,11-tetrahydroxy-1-methoxy-9,10-dihydro-7H-tetracene-5,12-dione
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TY - JOUR. T1 - Conformational folding and stability of the HET-C2 glycolipid transfer protein fold. T2 - Does a molten globule-like state regulate activity?. AU - Kenoth, Roopa. AU - Kamlekar, Ravi Kanth. AU - Simanshu, Dhirendra K.. AU - Gao, Yongguang. AU - Malinina, Lucy. AU - Prendergast, Franklyn G.. AU - Molotkovsky, Julian G.. AU - Patel, Dinshaw J.. AU - Venyaminov, Sergei Y.. AU - Brown, Rhoderick E.. PY - 2011/6/14. Y1 - 2011/6/14. N2 - The glycolipid transfer protein (GLTP) superfamily is defined by the human GLTP fold that represents a novel motif for lipid binding and transfer and for reversible interaction with membranes, i.e., peripheral amphitropic proteins. Despite limited sequence homology with human GLTP, we recently showed that HET-C2 GLTP of Podospora anserina is organized conformationally as a GLTP fold. Currently, insights into the folding stability and conformational states that regulate GLTP fold activity are almost nonexistent. To gain such insights into the ...
2-[anilino(phenyl)methyl]cyclohexan-1-one 737-47-3 NMR spectrum, 2-[anilino(phenyl)methyl]cyclohexan-1-one H-NMR spectral analysis, 2-[anilino(phenyl)methyl]cyclohexan-1-one C-NMR spectral analysis ect.
2. A particle according to claim 1, wherein the hydrophobic dye is represented by the following chemical formula (2): ##STR00014## where: L21, L22, L23, L24, L25, L26, and L27 may be identical to or different from one another and each represent CH or CR27; R27 represents a functional group selected from the group consisting of a halogen atom, an acetoxy group, an amino group, a nitro group, a cyano group, and an alkyl group having 1 to 18 carbon atoms; R27 may form a four-membered ring to a six-membered ring together with an alkyl group bonded to another L21, L22, L23, L24, L25, L26, or L27; X2 represents a counter ion required for neutralizing a charge of a molecule; p2 represents a number of X2s required for neutralizing a charge of an entire molecule; A21 represents any one of structures represented by the following chemical formula (3) and chemical formula (4); and A22 represents any one of structures represented by the following chemical formula (5) and chemical formula (6): ##STR00015## ...
The toxicity of Aromatic hydrocarbons, C10-13, reaction products with branched nonene, sulphonated, sodium salts to Daphnia magna was tested according to OECD TG 211 Daphnia magna reproduction test. Parental weight was the most sensitive parameter. EC10 for parental weight was reported to be 13.1 mg/L in the 21 day test under GLP conditions. This value equals to 6.9 mg/l based on the active ingredient concentration. The definitive daphnia test has been performed with the high nonene naphthalene sulphonate because algae and daphnia range finding tests with both the low nonene and high nonene substance indicated a slightly higher toxicity for the high nonene naphthalene sulphonate. As the results of both range finding tests are considered to be valid and because both test substances are very similar in their fate during these tests, the conclusion that the high nonene derivative is slightly more toxic than the low nonene derivative is considered sufficient to select the more toxic derivative as ...
Recombinant porin OmpF (an integral protein of bacterial outer membrane) from Yersinia pseudotuberculosis was synthesized in Escherichia coli cells as inclusion bodies. By combining the methods of anion-exchange and gel filtration chromatographies, recombinant OmpF (rOmpF) was isolated as an individual protein in its denatured state, and its characteristic properties (molecular mass, N-terminal amino acid sequence, and hydrodynamic radius of the protein in 8 M urea solution) were determined. According to the data of gel filtration, dynamic light scattering, optical spectroscopy, and binding of the hydrophobic fluorescent probe 8-anilino-1-naphthalenesulfonic acid, the rOmpF is fully unfolded in 8 M urea and exists in random coil conformation ...
Suggested for water based inks.. MSDS(Pigment Red 49:1). Related Information. English alias: 15630: 1; CI Pigment Red 49, barium salt (2: 1); Barium lithol red; 2- (2-Hydroxy-1-naphthylazo) -1-naphthalenesulfonic acid barium salt (2: 1); Barium bis [2- (2-hydroxy-1-naphthylazo) -1-naphthalenesulfonate]; D & C Red No.12; Lithol Red BA; Pigment Red 49 (Ba); RED R; CI PIGMENT RED 49: 1; barium bis { 2-[(2Z) -2- (2-oxonaphthalen-1 (2H) -ylidene) hydrazinyl] naphthalene-1-sulfonate}; barium bis {2-[(E)-(2-hydroxynaphthalen-1-yl) diazenyl ] naphthalene-1-sulfonate ...
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superplasticizer แนฟทาลีนของวัตถุดิบ (SNF / FDN / PNS) มีผลต่อการกระจายตัวของอนุภาคของปูนซีเมนต์และผลการใช้งานที่ดีในการเตรียมคอนกรีตที่ไหลผ่านและคอนกรีต. Quotation ...
2,4-Bis anilino pyrimidine deriv. 8 | C21H24ClN5O2 | CID 6538992 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.
PRO 2000 is a naphthalene sulfonate polymer that was being developed by Endo Pharmaceuticals Solutions (formerly Indevus Pharmaceuticals), in collaboration with
4-Methylsulfinyl-2,2-bi[1H-imidazole] | C7H8N4OS | CID 13600246 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.
1-anilino-2-phenyl-5,6-dihydro-1H-pyridin-4-one - chemical structural formula, chemical names, chemical properties, synthesis references
1-Amino-4-anilino-9,10-dihydro-9,10-dioxo-2-anthracenesulfonic acid/ACM2786712 can be provided in Alfa Chemistry. We are dedicated to provide our customers the best products and services.
1V1K: Cyclin-Dependent Kinase 4 Inhibitors as a Treatment for Cancer. Part 1: Identification and Optimisation of Substituted 4,6-Bis Anilino Pyrimidines
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28 articles were found. The following terms were included: anilino naphthalenesulfonate, 1-Anilino-8-naphthalin-sulfonat, Anilinonaphthalinsulfonat, ANS, 1-anilino-8-naphthalene sulfonate, アニリノナフタレンスルホン酸 ...
Here you can find all of the regulations and regulatory lists in which this substance appears, according to the data available to ECHA. This substance has been found in the following regulatory activities (directly, or inheriting the regulatory context of a parent substance):. ...
Coquillages et acidification des océans. Crevettes et acidification des océans. BRITISH ANTARTIC SURVEY 05/08/12 New study helps predict impact of ocean. Hazard
pls explain the ans...................................................... In facilitated diffusion, special proteins help move substances across membranes with
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CAS 130-37-0 2-Naphthalenesulfonic acid,1,2,3,4-tetrahydro-2-methyl-1,4-dioxo-, sodium salt * Golagen K * Hemoklot * Hetrogen K * Hetroge K premix * Hykinone * Ido-K * Kalzon * Kavitamin * Kavitan * Kawitan * Klotogen * Klotogen F * Klotogen F 16 * Klotogen F 227 * K-Trombina * Menadione sodium bisulfite * Menadione sodium hydrogen sulfite * Menaphthone sodium bisulfite * Menaphthone sodium bisulphite * MSBC * Sodium menadione bisulfite * 1,2,3,4-Tetrahydro-2-methyl-1,4-dioxo-2-naphthalenesulfonic acid sodium salt * Vikasol * Vitamin K injection * Vitamin K3 sodium bisulfite msds toxicity property
7-Benzamido-4-hydroxy-2-naphthalenesulfonic acid (CAS 132-87-6) Market Research Report 2018 aims at providing comprehensive data on 7-benzamido-4-hydroxy-2-naphthalenesulfonic
6-(4-Carboxyphenylamino)-4-hydroxy-2-naphthalenesulfonic acid (CAS 71486-49-2) Market Research Report 2018 aims at providing comprehensive data on 6-(4-carboxyphenylamino)-4-hydroxy-2-naphthalenesulfonic
93686-38-5 - Naphthalenesulfonic acid, dimethyl-, reaction products with formaldehyde, sodium salts - Searchable synonyms, formulas, resource links, and other chemical information.
90459-05-5 - Naphthalenesulfonic acid, reaction products with formaldehyde and toluene, ammonium salts - Searchable synonyms, formulas, resource links, and other chemical information.
ALPHA CHEMIKA is an ISO 9001:2000 certified manufacturer, supplier & exporter of 1-Amino-2-hydroxy-4-naphthalenesulfonic acid including all laboratory Chemicals, and offered to our clients at the best industry prices, Inquire us for more products details.
Name: 4-Hydroxy-6-(4-methoxyphenylamino)naphthalene-2-sulfonic acid CA Name: 2-Naphthalenesulfonic acid,4-hydroxy-6-[(4-methoxypheny)amino]-,monosodium salt Molecular Structure: 4-Hydroxy-6-(4-methoxyphenylamino)naphthalene-2-sulfonic acid,2-Naphthalenesulfonic acid,4-hydroxy-6-[(4-methoxypheny)amino]-,monosodium salt,CAS 201235-52-1,345.37,C17H15NO5S 4-Hydroxy-6-(4-methoxyphenylamino)naphthalene-2-sulfonic acid,2-Naphthalenesulfonic acid,4-hydroxy-6-[(4-methoxypheny)amino]-,monosodium salt,CAS 201235-52-1,345.37,C17H15NO5S Molecular Formula:C17H15NO5S Molecular Weight: 345.37 CAS Registry Number: 201235-52-1
Name: 8-Amino-5-(4-hydroxyphenylamino)naphthalene-2-sulfonic acid CA Name:2-Naphthalenesulfonic acid,8-amino-5-[(4-hydroxyphenyl)amino]- Molecular Structure: 8-Amino-5-(4-hydroxyphenylamino)naphthalene-2-sulfonic acid,2-Naphthalenesulfonic acid,8-amino-5-[(4-hydroxyphenyl)amino]-,CAS 6357-75-1,330.36,C16H14N2O4S 8-Amino-5-(4-hydroxyphenylamino)naphthalene-2-sulfonic acid,2-Naphthalenesulfonic acid,8-amino-5-[(4-hydroxyphenyl)amino]-,CAS 6357-75-1,330.36,C16H14N2O4S Molecular Formula:C16H14N2O4S Molecular Weight: 330.36 CAS Registry Number: 6357-75-1
2:00 pm A PYRIDINE-BASED TRIDENTATE CHELATING SOLVENT EXTRACTION SYSTEM FOR SELECTIVE EXTRACTION OF NICKEL AND COBALT: Batric Pesic1, and Taili Zhou2, 1University of Idaho, College of Mines-McClure Hall, Moscow, ID 83843; 2The Shepherd Chemical Co., 4900 Beech St., Cincinnati, OH 45212. A novel pyridine-based tridentate chelating extractant, 2,6bis-[5-n-nonylpyrazo-3-yl] pyridine (BNPP), has been developed and characterized. The solvent extraction of Ni and Co by a mixed system of BNPP and dinonyl naphthalene sulfonic acid (DNNSA) was studied as a function of pH, diluent, temperature, and DNNSA concentration. Stripping of Ni and Co was examined as a function of HCl and H2SO4 concentration. The novel system can extract Ni and/or Co selectively against Fe, Mn, Ca, Mg, and Al from acidic sulfate solutions at a pH as low as 0.5. Separation of Ni and Co can be achieved either during loading, or during stripping stages of solvent extraction. The extractant system is stable and can be regenerated with ...
[150 Pages Report] Check for Discount on Global and Chinese Sodium 2-naphthalenesulfonate (CAS 532-02-5) Industry, 2016 Market Research Report report by Prof Research. The Global and Chinese Sodium 2-naphthalenesulfonate Industry, 2011-2021...
This work describes the fluorescence enhancement of the anilinonaphthalene sulfonate probes 1,8-ANS, 2,6-ANS, and 2,6-TNS via complexation with PAMAM dendrimer hosts of Generation 4, 5 and 6. The use of this set of three very closely related probes allows for comparative binding studies, with specific pairs of probes differing only in shape (1,8-ANS and 2,6-ANS), or in the presence of a methyl substituent (2,6-TNS vs. 2,6-ANS). The fluorescence of all three probes was significantly enhanced upon binding with PAMAM dendrimers, however in all cases except one, a very unusual spike was consistently observed in the host fluorescence titration plots (fluorescence enhancement vs. host concentration) at low dendrimer concentration. This unprecedented fluorescence titration curve shape makes fitting the data to a simple model such as 1:1 or 2:1 host: guest complexation very difficult; thus only qualitative comparisons of the relative binding of the three guests could be made based on host titrations. In the
EINECS (European INventory of Existing Commercial chemical Substances) as published in O.J. C 146A, 15.6.1990. EINECS is an inventory of substances that were deemed to be on the European Community market between 1 January 1971 and 18 September 1981. EINECS was drawn up by the European Commission in the application of Article 13 of Directive 67/548/EEC, as amended by Directive 79/831/EEC, and in accordance with the detailed provisions of Commission Decision 81/437/EEC. Substances listed in EINECS are considered phase-in substances under the REACH Regulation ...
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice ...
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice ...
Population fI of the molten globule state of apomyoglobin mutants.Dependency of the population fI of the molten globule state versus urea concentration for apom
Enquiry for China 2,2-Bis(O-Chlorophenyl)-4,4,5,5-Tetraphenyl-1,2-Bi (III-Imidazole) 7189-82-4 of Eastar Chemical Corporation , China 2,2-Bis(O-Chlorophenyl)-4,4,5,5-Tetraphenyl-1,2-Bi (III-Imidazole) 7189-82-4 Suppliers & China 2,2-Bis(O-Chlorophenyl)-4,4,5,5-Tetraphenyl-1,2-Bi (III-Imidazole) 7189-82-4 Manufacturers
There are various fluorescent probes such as ANS (anilinonapthalene 8-sulphonate) and N-methyl-2-anilino-6-naphthalene sulphonate (MNS). They both contain charged and hydrophobic areas and therefore are situated at the water-lipid interface of the membrane. The fluorescent properties of the molecule vary with its mobility and also with the polarity of the environment. Studies with the ANS probe has shown that structural changes occur in mitochondrial membrane during oxidative phosphorylation. These probes have also helped in giving information about the structural features of the plasma membrane. ...
Tobias acid definition is - a crystalline naphthylaminesulfonic acid H2NC10H6SO3H used as a dye intermediate; 2-amino-1-naphthalenesulfonic acid.
Article Photo-Fenton-like treatment of K-acid: assessment of treatability, toxicity and oxidation products. Photo-Fenton-like treatment of the commercially important naphthalene sulphonate K-acid (2-naphthylamine-3,6,8-trisulphonic acid) was investig...
NAPHTHALENE-2,3-DICARBOXALDEHYDE 70848-82-7 MSDS report, NAPHTHALENE-2,3-DICARBOXALDEHYDE MSDS safety technical specifications search, NAPHTHALENE-2,3-DICARBOXALDEHYDE safety information specifications ect.
Using a high-throughput screening campaign, we identified the 4,6-bis anilino pyrimidines as inhibitors of the cyclin-dependent kinase, CDK4. Herein we describe the further chemical modification and use of X-ray crystallography to develop potent and selective in vitro inhibitors of CDK4. Cyclin-dependent kinase 4 inhibitors as a treatment for cancer. Part 1: identification and optimisation of substituted 4,6-bis anilino pyrimidines.,Beattie JF, Breault GA, Ellston RP, Green S, Jewsbury PJ, Midgley CJ, Naven RT, Minshull CA, Pauptit RA, Tucker JA, Pease JE Bioorg Med Chem Lett. 2003 Sep 15;13(18):2955-60. PMID:12941311[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine ...
Using a high-throughput screening campaign, we identified the 4,6-bis anilino pyrimidines as inhibitors of the cyclin-dependent kinase, CDK4. Herein we describe the further chemical modification and use of X-ray crystallography to develop potent and selective in vitro inhibitors of CDK4. Cyclin-dependent kinase 4 inhibitors as a treatment for cancer. Part 1: identification and optimisation of substituted 4,6-bis anilino pyrimidines.,Beattie JF, Breault GA, Ellston RP, Green S, Jewsbury PJ, Midgley CJ, Naven RT, Minshull CA, Pauptit RA, Tucker JA, Pease JE Bioorg Med Chem Lett. 2003 Sep 15;13(18):2955-60. PMID:12941311[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine ...
Confluency and cell viability can be estimated with optical or fluorescence microscopy using appropriate dyes for cell staining (e.g. neutral red). Note that some hydrophobic dyes may adsorb onto the scaffold. Quantitative, colorimetric assays (see next section) are also compatible and can provide a more accurate measurement of cell numbers within a scaffold. We recommend recording a growth curve before using a new cell line in the Mimetix scaffold to get a feel for its growth behaviour in 3D and how long it takes for the cells to become half-confluent or confluent.. ...
Suppliers List, E-mail/RFQ Form, Molecular Structure, Weight, Formula, IUPAC, Synonyms for Naphthalene-1,4,5,8-tetracarbonyl Chloride (CAS No. 37686-24-1)
If you have an incident to report at an event, you may contact the designated person listed in the event program. You may also contact ANS Executive Director Robert C. Fine, JD, CAE or 708-579-8200 or the ANS President at any time, including during and after events.. The complaint and investigation will be handled with respect for the privacy of all involved, and will be confidential to the extent practical, given the circumstances. Upon receiving a complaint, the matter may be further investigated by the Executive Director and/or the ANS President. Details of the complaint may later be shared with members of the Executive Committee and/or the Board of Directors, depending on the case. Individuals to be notified and actions to be taken will be discussed beforehand with the recipient of the harassing behavior, where possible.. Please note that ANS believes in respecting the wishes of those directly involved in the incident. While you may report an incident if you are not the target of the ...
WZ4003;1214265-58-3;N-[3-[5-chloro-2-[2-methoxy-4-(4-methylpiperazin-1-yl)anilino]pyrimidin-4-yl]oxyphenyl]propanamide;ABP001164.Active Biopharma Corp
View Notes - Homework #2 from PHYS 011 at HKUST. o , assuming an ideal op amp. [ans: mV 150 ] Q5. Plot the waveform of Vo(t). Q6. Fine V o and I s , assuming an ideal op amp. [ans: V o =10V, I s =5m]
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Medicinski pripomoček Vidermina CLX vaginalne globule pomagajo preprečevati vnetja vaginalnih predelov. Namenjene so osebni intimni negi žensk. Delujejo ...
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... the most spectacular case being the anilino naphthalene sulfonates (ANS); the first description of the use of the fluorescence ...
... (ANS), also called 1-anilino-8-naphthalenesulfonate, is an organic compound containing ...
... anilino naphthalenesulfonates MeSH D04.615.638.555.300 - congo red MeSH D04.615.638.555.400 - evans blue MeSH D04.615.638.555. ... naphthalenesulfonates MeSH D04.615.638.555.200 - amaranth dye MeSH D04.615.638.555.220 - amido black MeSH D04.615.638.555.282 ...
Effect of fatty acids on the binding of 1-anilino-8-naphthalenesulfonate to bovine serum albumin. Biochemistry. 1972 Jun 06; 11 ... Effect of fatty acids on the binding of 1-anilino-8-naphthalenesulfonate to bovine serum albumin. ... Effect of fatty acids on the binding of 1-anilino-8-naphthalenesulfonate to bovine serum albumin. ...
Magnesium 1-anilino-8-naphthalenesulfonate - Similar structures search, synonyms, formulas, resource links, and other chemical ... Substance Name: Magnesium 1-anilino-8-naphthalenesulfonate. RN: 18108-68-4. UNII: A9JT6A24EV. InChIKey: IRKGXNAZPUOEMW- ...
1-Anilino-8-naphthalenesulphonate Binding Parameters in Red Cell Membranes. Does Diabetes Mellitus Affect Cell Membrane ...
Matulis, D. and Lovrien, R. 1998 1-Anilino-8-naphthalene sulfonate anion-protein binding depends primarily on ion pair ...
Sarkar, P, Bharill, S, Gryczynski, I, Gryczynski, Z, Nair, MP & Lacko, AG 2008, Binding of 8-anilino-1-naphthalenesulfonate to ... Binding of 8-anilino-1-naphthalenesulfonate to lecithin:cholesterol acyltransferase studied by fluorescence techniques. Journal ... Binding of 8-anilino-1-naphthalenesulfonate to lecithin:cholesterol acyltransferase studied by fluorescence techniques. / ... Binding of 8-anilino-1-naphthalenesulfonate to lecithin:cholesterol acyltransferase studied by fluorescence techniques. ...
The binding of 1 anilino 8 naphthalenesulfonate (ANS) to human serum albumin has been studied and is shown to be consistent ... The binding of 1 anilino 8 naphthalenesulfonate (ANS) to human serum albumin has been studied and is shown to be consistent ... T2 - the use of 1 anilino 8 naphthalenesulphonate as a fluorescent probe for the study of the binding of iophenoxic and ... The binding of 1 anilino 8 naphthalenesulfonate (ANS) to human serum albumin has been studied and is shown to be consistent ...
Anilino Naphthalenesulfonates / metabolism * Anti-Bacterial Agents / pharmacology * Bacterial Proteins / metabolism * Cell ... PAβN treatment caused a significant increase in uptake of 8-anilino-1-naphthylenesulfonic acid, a fluorescent hydrophobic probe ...
5,5-bis(8-(phenylamino)-1-naphthalenesulfonate) Chemical Compounds * Tubulin Chemical Compounds ... In this study, we examined the effects of three anilinonaphthalenesulfonates on the interaction of tubulin with iodo[14C] ... Luduena RF, Roach MC, Horowitz P. The effects of the anilinonaphthalenesulfonates on the alkylation of tubulin: correlation ... Fingerprint Dive into the research topics of The effects of the anilinonaphthalenesulfonates on the alkylation of tubulin: ...
MBTH and 8-anilino-1-naphthalenesulfonate (ANS); or N-(3-sulfopropyl)aniline and MBTH; or other known and conventional dye ...
... fluorescent dyes such as umbelliferone and 1-anilino-8-naphthalenesulfonate; and luminol derivarives such as luminol, ...
8-anilino-1 -naphthalene sulfonate. ATF. Amino-terminal fragment. GFD. Growth factor-like domain ...
Anilino Naphthalenesulfonates/metabolism. *Circular Dichroism. *Electrophoresis, Gel, Two-Dimensional. *Electrophoresis, ... 8-anilino-1-naphthalenesulfate)-binding, far ultraviolet circular dichroism (UV-CD) spectral analysis, molecular sizes by ... 8-anilino-1-naphthalenesulfate)-binding, far ultraviolet circular dichroism (UV-CD) spectral analysis, molecular sizes by ...
4. The composition according to claim 3 wherein the third dye component is 8-anilino-1-naphthalenesulfonate or a salt thereof. ... The device according to claim 10 wherein the third dye component is 8-anilino-1-naphthalenesulfonate or a salt thereof. ... The method according to claim 17 wherein the third dye component is 8-anilino-1-naphthalenesulfonate or a salt thereof. ... 8-anilino-1-naphthalenesulfonate (ANS), or N-(3-sulfopropyl)aniline (HALPS). Other dye compounds which provide a sufficiently ...
An illustrative, but not limiting, example of a dye in that group is 1-anilino-8-naphthalene sulfonate (ANS). ANS has a low ... 2000) "Structural basis for the interaction of the fluorescence probe 8-anilino-1-naphthalene sulfonate (ANS) with the ...
1 -anilino-8-naphthalene sulfonate and 2-p-touidinyl-6-naphthalene sulfonate. Other dyes include 3-phenyl-7-isocyanatocoumarin ...
1-anilino-8-naphthalene sulfonate and 2-p-toluidinyl-6-naphthalene sulfonate. Other dyes include 3-phenyl-7-isocyanatocoumarin ... 4-anilino-1-naphthyl)maleimide; anthranilamide; BODIPY; Brilliant Yellow; coumarin and derivatives: coumarin, 7-amino-4- ...
A fluorescent probe of 1-anilino-8-naphthalene-sulfonate (82768) (ANS) was bound to synthetic phospholipid vesicles prepared ... A fluorescent probe of 1-anilino-8-naphthalene-sulfonate (82768) (ANS) was bound to synthetic phospholipid vesicles prepared ...
Haynes, D. H., and Staerck, H., 1974, 1-Anilino-8-naphthalenesulfonate: A fluorescent probe of membrane surface structure, ...
PDB reference: Hyp-1, a St Johns wort PR-10 protein in complex with the fluorescent probe 8-anilino-1-naphthalene sulfonate, ... was crystallized in complex with the fluorescent probe 8-anilino-1-naphthalene sulfonate (ANS). The asymmetric unit of the ...
The extrinsic fluor 1-anilino-8-naphthalene sulfonate (hereinafter "ANS") fluoresces when added to solutions of apohemoglobin ... naphthylamine sulfonic acids such as 1-anilino-8-naphthalene sulfonic acid ("ANS") and 2-p-toluidinylnaphthalene-6-sulfonic ...
Publications] 喜多村 昇: Energy Gap Dependence of the Nonradiative Decay Rate Constant of 1-Anilino-8-Naphthalene Sulfonate in ...
The extrinsic fluor 1-anilino-8-naphthalene sulfonate (hereinafter "ANS") fluoresces when added to solutions of apohemoglobin ... naphthylamine sulfonic acids such as 1-anilino-8-naphthalene sulfonic acid ("ANS") and 2-p-toluidinylnaphthalene-6-sulfonic ...
... the most spectacular case being the anilino naphthalene sulfonates (ANS); the first description of the use of the fluorescence ...
8-Anilinonaphthalene-1-sulfonic acid (ANS), also called 1-anilino-8-naphthalenesulfonate, is an organic compound containing ...
The interaction of 1‐anilino‐8‐naphthalene sulphonate with erythrocyte membranes. RAMJEESINGH et al. 1999. Novel method for ...
We had studied with ANS; 1-Anilino-8-Naphthalene Sulfonate as protein conformational tightening agent for LUSH protein folding ...
nm). The samples (20 μL volume) contained 5-10 μM protein, 0-200 μM compound, and 50 μM ANS (8-anilino-1-naphthalene sulfonate ...
Using tubulin-1-anilino-8-naphthalene sulfonate complex fluorescence, we have shown that LY290181 directly interacted with ...
Dimer formation from 1-anilino-8-naphthalenesulfonate catalyzed by bovine serum albumin. A new fluorescent molecule with ... Protein conformational changes induced by 1,1-Bis(4-anilino-5-napthalenesulfonic acid): Preferential binding to the molten ...
... we monitored the binding of 1-anilino-8-naphthalenesulfonate (ANS) (100 μM) to HdeA or HdeB (7 μM) by measuring the increase in ...
  • A fluorescent probe of 1-anilino-8-naphthalene-sulfonate (82768) (ANS) was bound to synthetic phospholipid vesicles prepared from egg lecithin and dimyristoyl-phosphatidyl-choline (DML). (cdc.gov)
  • Using tubulin-1-anilino-8-naphthalene sulfonate complex fluorescence, we have shown that LY290181 directly interacted with tubulin in a unique manner. (aspetjournals.org)
  • The conformational changes and thermostability were evaluated by circular dichroism spectroscopy, intrinsic tryptophan fluorescence, fluorescence quenching and 1-anilino-8-naphthalene sulfonate (ANS) fluorescence assay. (arvojournals.org)
  • The canine variant of the serum protein, plasminogen (DPgn) binds the fluorescent molecule, 8-anilino-l-naphthalene sulfonate (ANS). (concordia.ca)
  • Similarly, the non-substrate ligandin properties of the mutant were comparable to those of the wild-type protein as confirmed by 8-anilino-1-naphthalene sulfonate (ANS)-binding studies using active site ligands. (wits.ac.za)
  • Effect of various organic anions on the plasma disappearance of 1-anilino-8-naphthalene sulfonate. (statescale.cf)
  • Guanidinium HCl (GuHCl) chemical denaturations and 1-anilino-8-naphthalene sulfonate (ANS) binding of the pertuzumab Fabs. (labourlists.org)
  • Structural basis for the interaction of the fluorescence probe 8-anilino-1-naphthalene sulfonate (ANS) with the antibiotic target MurA. (mpg.de)
  • Here we describe the effect of FCCP on the binding of 1-anilino-8-naphthalene sulfonate (ANS) to 1, 2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) unilamellar vesicles. (gob.ar)
  • Studies with a range of constructs and mutants using intrinsic fluorescence, collision quenching, and extrinsic probe fluorescence (1-anilino-8-naphthalene sulfonate) show that the presence of the a' domain in full-length hPDI moderates the ability of the x-linker to generate the capped conformation (compared with shorter fragments) but does not abolish it. (warwick.ac.uk)
  • 8-Anilino naphthalene sulfonate sildenafil generico prezzo in farmacia binding as a probe for conformational changes induced in glutamate dehydrogenase by regulatory reagents. (svenskepiller.com)
  • The structural properties studied included dimerization and degradation, intrinsic tryptophan (Trp) fluorescence, ANS (8-anilino-1-naphthalenesulfate)-binding, far ultraviolet circular dichroism (UV-CD) spectral analysis, molecular sizes by dynamic light scattering, and oxidation of Trp and methionine (Met) residues. (nih.gov)
  • 1-anilino-8- naphthalene sulphonate (ANS) is a commonly used extrinsic chromophore which emits only weak fluorescence in polar environment, e.g. in aqueous solution. (news-medical.net)
  • We have developed a rapid and high throughput lipase-ANS (8-Anilino-l-naphthalenesulfonic acid) assay to evaluate the thermo-stability of lipases based on the ANS fluorescence signal's increasing and shifting when this small fluorescence probes binds to lipase. (bvsalud.org)
  • Valinomycin has been shown to increase the amount of 8-anilino-1- naphtha -lenesulfonate (ANS) bound to egg lecithin liposomes and also to increase the maximum fluorescence value, as derived from double reciprocal plots. (cnki.net)
  • Steroid hormone induced alterations in endometrium: Part II--On interaction of fluorescence probe, 1,8-anilino naphthalene sulphonic acid & endometrial mitochondria in health & disease. (bvsalud.org)
  • Geng, M.L. Generalized Two-Dimensional Hetero-correlation Analysis of Spectrally resolved and Temporally resolved Fluorescence of 8-Anilino-1-naphthalenesulfonate - Apomyoglobin Complex with pH Perturbation. (uiowa.edu)
  • There was also a dramatic increase in the binding of the hydrophobic fluorescent probe 1-anilino-8-naphthalenesulfonate during activation, leading us to conclude that a hydrophobic region in the protein is exposed by trypsin treatment. (epfl.ch)
  • Here, we show that the 86-kDa heterodimeric intermediate possesses a native-like conformation as judged by its binding to a fluorescent probe 1-anilino-8-naphthalenesulfonate. (termsreign.ga)
  • The solvatochromic fluorescent probe 8-anilino-1-naphthalenesulfonate (ANS) has been used to study the hydrophobicity and conformational dynamics of lecithin:cholesterol acyltransferase (LCAT). (unthsc.edu)
  • PAβN treatment caused a significant increase in uptake of 8-anilino-1-naphthylenesulfonic acid, a fluorescent hydrophobic probe, and sensitized P. aeruginosa to bulky antibiotics (e.g. vancomycin) that are normally incapable of crossing the outer membrane, as well as to detergent-like bile salts. (nih.gov)
  • The aim of the present study was to investigate the potential of fourth generation (P4) cationic phosphorus-containing dendrimers to bind fluorescent probe 8-anilino-1-naphthalenesulfonate (ANS), anti-neoplastic drug cisplatin, anti-HIV siRNA siP24 and its capability to deliver green fluorescent protein gene (pGFP) into cells. (mdpi.com)
  • We attempted to develop a fluorescent probe superior to conventional ones (8-anilino-1-naphthalenesulfonate and auramine O) for use in the study of drug-binding sites on alpha 1-acid glycoprotein (AGP). (sigmaaldrich.com)
  • 3. The composition according to claim 1 further comprising a third dye component selected from the group consisting of 3,3-methylaminobenzoic acid, 3,5-dichloro-2-hydroxybenzenesulfonic acid, 8-anilino-1-naphthelenesulfonate, N-(3-sulfopropyl)aniline or salts thereof. (google.com)
  • 10. The device according to claim 8 wherein the dry chemistry reagent system further comprises a third dye component selected from the group consisting of 3,3-dimethylaminobenzoic acid, 3,5-dichloro-2-hydroxybenzenesulfonic acid, 8-anilino-1-naphthelenesulfonate, N-(3-sulfopropyl)aniline or salts thereof. (google.com)
  • 8-Anilinonaphthalene-1-sulfonic acid (ANS), also called 1-anilino-8-naphthalenesulfonate, is an organic compound containing both a sulfonic acid and an amine group. (wikipedia.org)
  • Two small molecules, the paramagnetic relaxation agent 4-hydroxy-2,2,6,6-tetramethylpiperidinyl-1-oxy (HyTEMPO) and the fluorescent dye 8-anilino-1-naphthalenesulfonic acid (ANS), were used to alter and simplify the spectrum. (nih.gov)
  • A class of organic compounds which contain an anilino (phenylamino) group linked to a salt or ester of naphthalenesulfonic acid. (bvsalud.org)
  • There are various fluorescent probes such as ANS (anilinonapthalene 8-sulphonate) and N-methyl-2-anilino-6-naphthalene sulphonate (MNS). (blogspot.in)
  • The hemolytic activities of both proteins were similarly potentiated with 8-anilino-1-naphthalenesulfonate (ANS). (nus.edu.sg)
  • Effect of fatty acids on the binding of 1-anilino-8-naphthalenesulfonate to bovine serum albumin. (harvard.edu)
  • In this study, we examined the effects of three anilinonaphthalenesulfonates on the interaction of tubulin with iodo[ 14 C]acetamide and N,N′-ethylenebis(iodoacetamied). (uthscsa.edu)
  • Luduena, RF, Roach, MC & Horowitz, P 1986, ' The effects of the anilinonaphthalenesulfonates on the alkylation of tubulin: correlation between the appearance of sulfhydryl groups and apolar binding sites ', Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular , vol. 873, no. 1, pp. 143-146. (uthscsa.edu)
  • b) The enhansive and suppressive effects of 8-anilino-1-naphthalenesulfonate, NiCl2, and uranyl acetate on the responses to anodal current were similar to those on the responses to chemical stimulation. (rupress.org)
  • 4. The composition according to claim 3 wherein the third dye component is 8-anilino-1-naphthalenesulfonate or a salt thereof. (google.com)