Proteins that specifically inhibit the growth of new blood vessels (ANGIOGENESIS, PHYSIOLOGIC).
Agents and endogenous substances that antagonize or inhibit the development of new blood vessels.
A pathologic process consisting of the proliferation of blood vessels in abnormal tissues or in abnormal positions.
A CXC chemokine that is induced by GAMMA-INTERFERON and is chemotactic for MONOCYTES and T-LYMPHOCYTES. It has specificity for the CXCR3 RECEPTOR.
Circulating 38-kDa proteins that are internal peptide fragments of PLASMINOGEN. The name derives from the fact that they are potent ANGIOGENESIS INHIBITORS. Angiostatins contain four KRINGLE DOMAINS which are associated with their potent angiostatic activity.
Angiostatic proteins that are formed from proteolytic cleavage of COLLAGEN TYPE XVIII.
A CXC chemokine that is found in the alpha granules of PLATELETS. The protein has a molecular size of 7800 kDa and can occur as a monomer, a dimer or a tetramer depending upon its concentration in solution. Platelet factor 4 has a high affinity for HEPARIN and is often found complexed with GLYCOPROTEINS such as PROTEIN C.
CXCR receptors that are expressed on the surface of a number of cell types, including T-LYMPHOCYTES; NK CELLS; DENDRITIC CELLS; and a subset of B-LYMPHOCYTES. The receptors are activated by CHEMOKINE CXCL9; CHEMOKINE CXCL10; and CHEMOKINE CXCL11.
Doubly unsaturated pregnane derivatives with two hydroxy groups substituted anywhere on the rings or side chains.
A CXC chemokine that is induced by GAMMA-INTERFERON. It is a chemotactic factor for activated T-LYMPHOCYTES and has specificity for the CXCR3 RECEPTOR.
An extracellular matrix glycoprotein from platelets and a variety of normal and transformed cells of both mesenchymal and epithelial origin. Thrombospondin-1 is believed to play a role in cell migration and proliferation, during embryogenesis and wound repair. Also, it has been studied for its use as a potential regulator of tumor growth and metastasis.
The pathological process occurring in cells that are dying from irreparable injuries. It is caused by the progressive, uncontrolled action of degradative ENZYMES, leading to MITOCHONDRIAL SWELLING, nuclear flocculation, and cell lysis. It is distinct it from APOPTOSIS, which is a normal, regulated cellular process.
A heterogeneous group of sporadic or hereditary carcinoma derived from cells of the KIDNEYS. There are several subtypes including the clear cells, the papillary, the chromophobe, the collecting duct, the spindle cells (sarcomatoid), or mixed cell-type carcinoma.
Organic salts and esters of benzenesulfonic acid.
Compounds that include the amino-N-phenylamide structure.
Tumors or cancers of the KIDNEY.
An important compound functioning as a component of the coenzyme NAD. Its primary significance is in the prevention and/or cure of blacktongue and PELLAGRA. Most animals cannot manufacture this compound in amounts sufficient to prevent nutritional deficiency and it therefore must be supplemented through dietary intake.
Exclusive legal rights or privileges applied to inventions, plants, etc.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
A novel composition, device, or process, independently conceived de novo or derived from a pre-existing model.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Property, such as patents, trademarks, and copyright, that results from creative effort. The Patent and Copyright Clause (Art. 1, Sec. 8, cl. 8) of the United States Constitution provides for promoting the progress of science and useful arts by securing for limited times to authors and inventors, the exclusive right to their respective writings and discoveries. (From Black's Law Dictionary, 5th ed, p1014)
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The adherence and merging of cell membranes, intracellular membranes, or artificial membranes to each other or to viruses, parasites, or interstitial particles through a variety of chemical and physical processes.
A genetic process by which the adult organism is realized via mechanisms that lead to the restriction in the possible fates of cells, eventually leading to their differentiated state. Mechanisms involved cause heritable changes to cells without changes to DNA sequence such as DNA METHYLATION; HISTONE modification; DNA REPLICATION TIMING; NUCLEOSOME positioning; and heterochromatization which result in selective gene expression or repression.
The repeating structural units of chromatin, each consisting of approximately 200 base pairs of DNA wound around a protein core. This core is composed of the histones H2A, H2B, H3, and H4.
The systematic study of the global gene expression changes due to EPIGENETIC PROCESSES and not due to DNA base sequence changes.
Addition of methyl groups to DNA. DNA methyltransferases (DNA methylases) perform this reaction using S-ADENOSYLMETHIONINE as the methyl group donor.
Small chromosomal proteins (approx 12-20 kD) possessing an open, unfolded structure and attached to the DNA in cell nuclei by ionic linkages. Classification into the various types (designated histone I, histone II, etc.) is based on the relative amounts of arginine and lysine in each.
New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.
Antibodies produced by a single clone of cells.
A form of antibodies consisting only of the variable regions of the heavy and light chains (FV FRAGMENTS), connected by a small linker peptide. They are less immunogenic than complete immunoglobulin and thus have potential therapeutic use.
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
That region of the immunoglobulin molecule that varies in its amino acid sequence and composition, and comprises the binding site for a specific antigen. It is located at the N-terminus of the Fab fragment of the immunoglobulin. It includes hypervariable regions (COMPLEMENTARITY DETERMINING REGIONS) and framework regions.
A soluble substance elaborated by antigen- or mitogen-stimulated T-LYMPHOCYTES which induces DNA synthesis in naive lymphocytes.
A condition in which functional endometrial tissue is present outside the UTERUS. It is often confined to the PELVIS involving the OVARY, the ligaments, cul-de-sac, and the uterovesical peritoneum.
Publications printed and distributed daily, weekly, or at some other regular and usually short interval, containing news, articles of opinion (as editorials and letters), features, advertising, and announcements of current interest. (Webster's 3d ed)
Instruments or technological means of communication that reach large numbers of people with a common message: press, radio, television, etc.
Services providing pharmaceutic and therapeutic drug information and consultation.
An agency of the NATIONAL INSTITUTES OF HEALTH concerned with overall planning, promoting, and administering programs pertaining to advancement of medical and related sciences. Major activities of this institute include the collection, dissemination, and exchange of information important to the progress of medicine and health, research in medical informatics and support for medical library development.
Drugs manufactured and sold with the intent to misrepresent its origin, authenticity, chemical composition, and or efficacy. Counterfeit drugs may contain inappropriate quantities of ingredients not listed on the label or package. In order to further deceive the consumer, the packaging, container, or labeling, may be inaccurate, incorrect, or fake.
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
Administrative units of government responsible for policy making and management of governmental activities.
A computerized biomedical bibliographic storage and retrieval system operated by the NATIONAL LIBRARY OF MEDICINE. MEDLARS stands for Medical Literature Analysis and Retrieval System, which was first introduced in 1964 and evolved into an online system in 1971 called MEDLINE (MEDLARS Online). As other online databases were developed, MEDLARS became the name of the entire NLM information system while MEDLINE became the name of the premier database. MEDLARS was used to produce the former printed Cumulated Index Medicus, and the printed monthly Index Medicus, until that publication ceased in December 2004.
A short thick vein formed by union of the superior mesenteric vein and the splenic vein.
Tumors or cancer of the COLON or the RECTUM or both. Risk factors for colorectal cancer include chronic ULCERATIVE COLITIS; FAMILIAL POLYPOSIS COLI; exposure to ASBESTOS; and irradiation of the CERVIX UTERI.
Non-collagenous, calcium-binding glycoprotein of developing bone. It links collagen to mineral in the bone matrix. In the synonym SPARC glycoprotein, the acronym stands for Secreted Protein, Acidic and Rich in Cysteine.
The milieu surrounding neoplasms consisting of cells, vessels, soluble factors, and molecules, that can influence and be influenced by, the neoplasm's growth.
A cell line derived from cultured tumor cells.
A CCN protein family member that regulates a variety of extracellular functions including CELL ADHESION; CELL MIGRATION; and EXTRACELLULAR MATRIX synthesis. It may play an important role in the development of branched CAPILLARIES during EMBRYOGENESIS.
Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.

Platelets release CXCL4L1, a nonallelic variant of the chemokine platelet factor-4/CXCL4 and potent inhibitor of angiogenesis. (1/25)

Platelet factor-4 (PF-4)/CXCL4 was the first chemokine described to inhibit neovascularization. Here, the product of the nonallelic variant gene of CXCL4, PF-4var1/PF-4alt, designated CXCL4L1, was isolated for the first time from thrombin-stimulated human platelets and purified to homogeneity. Although secreted CXCL4 and CXCL4L1 differ in only three amino acids, CXCL4L1 was more potent in inhibiting chemotaxis of human microvascular endothelial cells toward interleukin-8 (IL-8)/CXCL8 or basic fibroblast growth factor (bFGF). In vivo, CXCL4L1 was also more effective than CXCL4 in inhibiting bFGF-induced angiogenesis in rat corneas. Thus, activated platelets release CXCL4L1, a potent regulator of endothelial cell biology, which affects angiogenesis and vascular diseases.  (+info)

BMP-1/Tolloid-like metalloproteases process endorepellin, the angiostatic C-terminal fragment of perlecan. (2/25)

Endorepellin, the C-terminal domain of the heparan sulfate proteoglycan perlecan, possesses angiostatic activity. The terminal laminin-like globular (LG3) domain of endorepellin appears to possess most of the biological activity on endothelial cells. LG3 protein has been detected in the urine of patients with end-stage renal disease and in the amniotic fluid of pregnant women with premature rupture of fetal membranes. These findings suggest that proteolytic processing of endorepellin and the generation of LG3 might have biological significance. In this study, we have identified specific enzymes of the bone morphogenetic protein-1 (BMP-1)/Tolloid family of metalloproteases that cleave LG3 from recombinant endorepellin at the physiologically relevant site and that cleave LG3 from endogenous perlecan in cultured mouse and human cells. The BMP-1/Tolloid family of metalloproteases is thereby implicated in the processing of a major basement membrane proteoglycan and in the liberation of an anti-angiogenic factor. Using molecular modeling, site-directed mutagenesis and angiogenic assays, we further demonstrate that LG3 activity requires specific amino acids involved in Ca(2+) coordination.  (+info)

Angiostatic peptides use plasma fibronectin to home to angiogenic vasculature. (3/25)

A group of angiogenesis inhibitors are derived from fragments of extracellular matrix or blood proteins. Endostatin, antithrombin, and anastellin are members of this group of substances. The plasma adhesion proteins fibronectin and vitronectin serve as cofactors for these three antiangiogenic proteins. Anginex is a synthetic 33-amino acid peptide that was originally modeled to reproduce the beta-sheet structure of antiangiogenic proteins. Here, we show that anginex initiates fibronectin polymerization and is inactive in mice that lack plasma fibronectin. Anginex shares these characteristics with anastellin. Fluorescein-labeled anginex and anastellin specifically localized in angiogenic vessels in vivo. This localization was dependent on plasma fibronectin and inhibited by an Arg-Gly-Asp peptide. Thus, anginex shares with several physiological angiogenesis inhibitors a dependence on plasma adhesion proteins. The role of the adhesion protein interaction apparently is to form integrin-binding complexes that deliver the antiangiogenic proteins to sites of angiogenesis. This functional convergence of several antiangiogenic factors has important implications for antiangiogenic therapies.  (+info)

Identification of CD36 molecular features required for its in vitro angiostatic activity. (4/25)

Thrombospondin-1 (TSP-1), a natural inhibitor of angiogenesis, acts directly on endothelial cells (EC) via CD36 to inhibit their migration and morphogenesis induced by basic fibroblast growth factor. Here we show that CD36 triggered by TSP-1 inhibits in vitro angiogenesis stimulated by vascular endothelial growth factor-A (VEGF-A). To demonstrate that the TSP-1 inhibitory signal was mediated by CD36, we transduced CD36 in CD36-deficient endothelial cells. Both TSP-1 and the agonist anti-CD36 mAb SMO, which mimics TSP-1 activity, reduced the VEGF-A165-induced migration and sprouting of CD36-ECs. To address the mechanisms by which CD36 may exert its angiostatic function, we investigated the functional components of the C-terminal cytoplasmic tail by site-directed mutagenesis. Our results indicate that C464, R467, and K469 of CD36 are required for the inhibitory activity of TSP-1. In contrast, point mutation of C466 did not alter TSP-1 ability to inhibit EC migration and sprouting. Moreover, we show that activation of CD36 by TSP-1 down-modulates the VEGF receptor-2 (VEGFR-2) and p38 mitogen-associated protein kinase phosphorylation induced by VEGF-A165, and this effect was specifically abolished by point mutation at C464. These results identify specific amino acids of the C-terminal cytoplasmic tail of CD36 crucial for the in vitro angiostatic activity of TSP-1 and extend our knowledge of regulation of VEGFR-2-mediated biological activities on ECs.  (+info)

T2-TrpRS inhibits preretinal neovascularization and enhances physiological vascular regrowth in OIR as assessed by a new method of quantification. (5/25)

PURPOSE: A carboxyl-terminal fragment of tryptophan tRNA synthetase (T2-TrpRS) has demonstrated potent angiostatic activity during retinal developmental neovascularization in vivo. The effects of T2-TrpRS on pathologic neovascularization were tested and compared with a potent VEGF antagonist using the mouse model of oxygen-induced retinopathy (OIR). METHODS: C57BL/6J mice were transiently exposed to hyperoxic conditions (75% O2) between postnatal day 7 (P7) and P12 and then returned to room air. Retinas were isolated, blood vessels stained with isolectin Griffonia simplicifolia, images of retinal whole-mounts acquired, and the area of vascular obliteration and extent of preretinal neovascularization quantified. This method was compared to the commonly used method of OIR quantification in which the number of pre-inner limiting membrane (ILM) nuclei is counted in serial sections of whole eyes. To assess the angiostatic activity of T2-TrpRS, mice were injected intravitreally at P12 with either T2-TrpRS, a VEGF aptamer, or vehicle (PBS) alone, and the effects on area of obliteration and on preretinal neovascular tuft formation were assessed. RESULTS: Using a modified method of quantification in the mouse OIR model based on images of isolectin-stained retinal wholemounts, we were able to assess reliably and consistently both vascular obliteration and preretinal neovascular tuft formation in the same specimen. T2-TrpRS demonstrated potent angiostatic activity, reducing the appearance of pathologic neovascular tufts by up to 90%. Surprisingly, T2-TrpRS also enhanced physiological revascularization of the obliterated retinal vasculature, reducing these areas by up to 60% compared with PBS-injected eyes. In contrast, the VEGF antagonist, while similarly reducing preretinal neovascular tuft formation, did not enhance revascularization of the obliterated areas. CONCLUSIONS: Use of a rapid, quantifiable method to assess the effect of T2-TrpRS on retinal angiogenesis in the OIR model demonstrates the importance of a quantification system that permits simultaneous analysis of a drug's effect on vascular obliteration as well as on preretinal neovascularization. The results obtained using this method suggest enhanced clinical value for compounds such as T2-TrpRS that not only inhibit pathologic neovascularization, but also facilitate physiological revascularization of ischemic tissue.  (+info)

Endorepellin, the C-terminal angiostatic module of perlecan, enhances collagen-platelet responses via the alpha2beta1-integrin receptor. (6/25)

Endorepellin, a C-terminal fragment of the vascular basement membrane proteoglycan perlecan, inhibits angiogenesis via the alpha2beta1-integrin receptor. Because this integrin is also implicated in platelet-collagen responses and because endorepellin or its fragments are generated in response to injury and inflammation, we hypothesized that endorepellin could also affect platelet biology. We discovered that endorepellin supported alpha2beta1-dependent platelet adhesion, without appreciably activating or aggregating platelets. Notably, endorepellin enhanced collagen-evoked responses in platelets, in a src kinase-dependent fashion, and enhanced the collagen-inhibitory effect of an alpha2beta1-integrin function-blocking antibody. Collectively, these results suggest that endorepellin/alpha2beta1-integrin interaction and effects are specific and dependent on cell type, differ from those emanated by exposure to collagen, and may be due to cellular differences in alpha2beta1-integrin activation/ligand affinity state. These studies also suggest a heretofore unrecognized role for angiostatic basement membrane fragments in platelet biology.  (+info)

Clinical implications of angiogenesis in cancers. (7/25)

Angiogenesis plays an important role in the growth and progression of cancer. The regulation of tumor angiogenesis depends on a net balance of angiogenic factors and antiangiogenic factors, which are secreted by both tumor cells and host-infiltrating cells. Numerous studies have indicated that assessment of angiogenic activity by either microvessel density or expression of angiogenic factors in cancer can provide prognostic information independent of conventional clinicopathological factors such as tumor staging. Some studies also suggested that assessment of tumor angiogenesis may predict cancer response to chemotherapy or radiotherapy. However, the most important clinical implication of tumor angiogenesis is the development of a novel strategy of anticancer therapy targeting tumor vessels instead of cancer cells. Antiangiogenic therapy aims to inhibit the growth of tumor, and current evidence suggests that it works best in combination with conventional cytotoxic chemotherapy. Recently, a monoclonal antibody against vascular endothelial growth factor, which is one of the most potent angiogenic factors, has been approved for clinical use in colorectal cancer patients after a clinical trial confirmed that combining the antibody with standard chemotherapy regimen could prolong patient survival. The clinical implications of angiogenesis in cancer are reviewed in this article.  (+info)

Stimulation of angiostatic platelet factor-4 variant (CXCL4L1/PF-4var) versus inhibition of angiogenic granulocyte chemotactic protein-2 (CXCL6/GCP-2) in normal and tumoral mesenchymal cells. (8/25)

Chemokines affect inflammation and cancer through leukocyte attraction and angiogenesis. Here, we demonstrate that CXCL4L1/platelet factor-4 variant (PF-4var), a highly angiostatic chemokine, is poorly chemotactic for phagocytes and is inducible in monocytes by inflammatory mediators but remained undetectable in macrophages and neutrophils. In addition, CXCL4L1/PF-4var production by mesenchymal tumor cells was evidenced in vitro and in vivo by specific ELISA and immunohistochemistry. CXCL4L1/PF-4var, but not CXCL4/PF-4, was coinduced with the angiogenic chemokine CXCL6/granulocyte chemotactic protein-2 (GCP-2) by cytokines, e.g., IL-1beta and IL-17, in sarcoma cells, but not in diploid fibroblasts. Furthermore, the induction of CXCL6/GCP-2 in endothelial cells by IL-1beta was enhanced synergistically by TNF-alpha but inhibited by IFN-gamma, which synergized with IL-1beta to produce the angiostatic CXCL10/IFN-gamma-induced protein-10. These findings indicate that the equilibrium between angiostatic and angiogenic factors during inflammation and tumor progression is rather complex and differs depending on the chemokine, cell type, and stimulus. Selective intervention in the chemokine network may drastically disturb this delicate balance of angiogenesis and tissue repair. Application of angiostatic CXCL4L1/PF-4var without attraction of protumoral phagocytes may be beneficial in cancer therapy.  (+info)

Reactome is pathway database which provides intuitive bioinformatics tools for the visualisation, interpretation and analysis of pathway knowledge.
La Jolla, CA-Pfizer Inc. has acquired Angiosyn Inc., a privately held biopharmaceutical company developing an angiostatic agent for ophthalmic diseases, including age-related macular degeneration (AMD).
Abstract Introduction: The role of platelet activation in allergic inflammation is receiving increasing attention. Sublingual immunotherapy for allergic rhinitis can modify the immunological process to an allergen, rather than simply treating symptoms. Objective: The aim of this study was to explore the role of platelet activation during sublingual immunotherapy in children with allergic rhinitis. Methods: Forty-two House Dust Mite - sensitized children with allergic rhinitis were enrolled and received House Dust Mite allergen extract for sublingual immunotherapy or placebo. Serum of different time points during treatment was collected and used for detection of Platelet Factor-4 and Beta-Thromboglobulin concentration by Enzyme-Linked Immuno Sorbent Assay. Results: Our data showed decreased expression of Platelet Factor-4 and Beta-Thromboglobulin protein after one years sublingual immunotherapy. In addition, the decrease of symptom scores and serum Platelet Factor-4 and Beta-Thromboglobulin protein
Berlin Germany: Chemicals that inhibit the development of new blood v...However the researchers warned that the work is still at an early sta...Endometriosis is a disease of the lining tissue of the womb. It can b...Annemiek Nap a doctor at the University Hospital Maastricht The Neth...They used four angiostatic compounds: anti-human vascular endothelial ...,Endometriosis:,Could,angiostatic,therapy,be,the,new,treatment,of,the,future?,biological,biology news articles,biology news today,latest biology news,current biology news,biology newsletters
TY - JOUR. T1 - Fibroblast Growth Factor-2 Antagonist Activity and Angiostatic Capacity of Sulfated Escherichia coli K5 Polysaccharide Derivatives. AU - Leali, Daria. AU - Belleri, Mirella. AU - Urbinati, Chiara. AU - Coltrini, Daniela. AU - Oreste, Pasqua. AU - Zoppetti, Giorgio. AU - Ribatti, Domenico. AU - Rusnati, Marco. AU - Presta, Marco. PY - 2001/10/12. Y1 - 2001/10/12. N2 - The angiogenic basic fibroblast growth factor (FGF2) interacts with tyrosine kinase receptors (FGFRs) and heparan sulfate proteoglycans (HSPGs) in endothelial cells. Here, we report the FGF2 antagonist and antiangiogenic activity of novel sulfated derivatives of the Escherichia coli K5 polysaccharide. K5 polysaccharide was chemically sulfated in N- and/or O-position after N-deacetylation. O-Sulfated and N,O-sulfated K5 derivatives with a low degree and a high degree of sulfation compete with heparin for binding to 125I-FGF2 with different potency. Accordingly, they abrogate the formation of the HSPG-FGF2-FGFR ternary ...
Chemokines belong to a class of pro-inflammatory cytokines that have the ability to attract and activate leukocytes. Their name is derived from chemotactic cytokines based on their ability to induce and mediate chemotaxis in nearby responsive cells. Formerly, they were called SIS family of cytokines, SIG family of cytokines, SCY family of cytokines, Platelet factor-4 superfamily or intercrines. Chemokines can be divided into at least four structural branches: c (chemokines, c), cc (chemokines, cc), cx3c (chemokines, cx3c), and cxc (chemokines, cxc). The classification is according to the variations in a shared cysteine motif. Chemokines may also be classified based on their functions. Homeostatic chemokines are chemokines that are responsible for basal leukocyte migration. Examples of homeostatic chemokines are CCL14, CCL19, CCL20, CCL21, CXCL12 and CXCL13. Nevertheless, some of them are not exclusive to this function. For instance, CCL20 is also associated with inflammation since it can act as ...
Chemokines (Greek -kinos, movement) are a family of small cytokines, or signaling proteins secreted by cells. Their name is derived from their ability to induce directed chemotaxis in nearby responsive cells; they are chemotactic cytokines. Cytokine proteins are classified as chemokines according to behavior and structural characteristics. In addition to being known for mediating chemotaxis, chemokines are all approximately 8-10 kilodaltons in mass and have four cysteine residues in conserved locations that are key to forming their 3-dimensional shape. These proteins have historically been known under several other names including the SIS family of cytokines, SIG family of cytokines, SCY family of cytokines, Platelet factor-4 superfamily or intercrines. Some chemokines are considered pro-inflammatory and can be induced during an immune response to recruit cells of the immune system to a site of infection, while others are considered homeostatic and are involved in controlling the migration of ...
In the present study, it was demonstrated that IL-17A could stimulate the secretion of angiogenic CXC chemokines from liver cancer cells, which may recruit endothelial cells to the tumor cells in a CXCR2-dependent manner. Tumor angiogenesis was also promoted by IL-17A expression in vivo. The CXC chemokines can be classified as angiogenic or angiostatic predominantly based on the presence or absence of an ELR motif. The angiogenic CXC chemokines include CXCL1, CXCL2, CXCL3, CXCL5, CXCL6, CXCL8 and CXCL12, and the angiostatic chemokines include CXCL4, CXCL9, CXCL10, CXCL11 and CXCL14 (21). IL-17A was shown to increase the expression of CXCL1, CXCL2, CXCL3, CXCL5, CXCL6 and CXCL8 in Huh7.5 cells and upregulated CXCL2 in HepG2 cells. Additionally, angiostatic CXC chemokines were not affected by IL-17A in both cell lines. IL-17A has been reported to stimulate VEGFA production and promote angiogenesis in several cancer cell lines (16-18) and it has been shown previously that IL-17A does not affect ...
Cancer cells are often dependent on epigenetic pathways for their survival. Consequently, drugs that target the epigenome, rather than the underlying DNA sequence, are currently attracting considerabl
In the Mouse Antithrombotic Assay aspirin (30-300 mg/kg) protected mice from death by 15 and 42%, respectively. Four Ca++ channel blockers (nitrendipine, nicardipine, nifedipine and verapamil) were effective in reducing the mortality. At the dose of 100 mg/kg nitrendipine and nicardipine gave 80 and 85% protection respectively. Whereas aspirin almost suppressed completely thromboxane (Tx)B2 and platelet factor-4 release after collagen-epinephrine infusion, neither nitrendipine nor nicardipine modified TxB2 release and only reduced slightly platelet factor-4 release. The treatment with aspirin, nitrendipine or nicardipine did not counteract the fall in circulating platelets counted 1 min after the aggregation challenge, but at 3 min platelet count was significantly higher in aspirin-treated mice than in animals given either Ca++ channel blocker. Mouse platelet aggregation induced in vitro by the combination of collagen and epinephrine was inhibited in samples obtained from mice pretreated with ...
To examine whether the lack of sufficient neoangiogenesis in systemic sclerosis (SSc) is caused by a decrease in angiogenic factors and/or an increase in angiostatic factors, the potent proangiogenic molecules vascular endothelial growth factor (VEGF) and basic fibroblast growth factor, and the angiostatic factor endostatin were determined in patients with SSc and in healthy controls. Forty-three patients with established SSc and nine patients with pre-SSc were included in the study. Serum levels of VEGF, basic fibroblast growth factor and endostatin were measured by ELISA. Age-matched and sex-matched healthy volunteers were used as controls. Highly significant differences were found in serum levels of VEGF between SSc patients and healthy controls, whereas no differences could be detected for endostatin and basic fibroblast growth factor. Significantly higher levels of VEGF were detected in patients with Scl-70 autoantibodies and in patients with diffuse SSc. Patients with pre-SSc and short ...
Background: Sepsis-associated disseminated intravascular coagulation (SAC) is associated with marked hemostatic changes including transient thrombocytopenia due to their enodogenous activation / consumption. The widespread activation of platelets contribute to vascular occlusions, fibrin deposition, multi-organ dysfunction, contributing to a two-fold increase in mortality.. Aims: The purpose of this study was to measure markers of platelet function in the plasma of patients with clinically established SAC and to determine their association to disease severity and outcome.. Methods: Plasma samples from 103 adult intensive care unit (ICU) patients with sepsis and suspected disseminated intravascular coagulation (DIC) were acquired from the University of Utah Hospital at the time of admission. Samples were shipped to Loyola University Chicago and stored at -80˚C prior to analysis. Plasma levels of CD40L, von Willebrand Factor (vWF), platelet factor-4 (PF-4), and microparticles (MP) were quantified ...
Neutrophil-activating protein-2 (NAP-2) is a 72 residue protein demonstrating a range of proinflammatory activities. The solution structure of monomeric NAP-2 has been investigated by two-dimensional 1H-n.m.r. spectroscopy. Sequence-specific proton resonance assignments have been made and secondary structural elements have been identified on the basis of nuclear Overhauser data, coupling constants and amide hydrogen/deuteron exchange. The NAP-2 monomer consists of a triple-stranded anti-parallel beta-sheet arranged in a Greek key and a C-terminal helix (residues 59-70) and is very similar to that found in the n.m.r. solution conformation of dimeric interleukin-8 and the crystal structure of tetrameric bovine platelet factor-4. Results are discussed in terms of heparin binding and neutrophil-activation properties of NAP-2. ...
The purpose of this study is to evaluate the efficacy, tolerability and safety of a multi-targeted therapy in patients with hormone-refractory prostate
Multivariate normal distribution theory, correlation and dependence analysis, regression and prediction, dimension-reduction methods, sampling distributions and related inference problems, selected applications in classification theory, multivariate process control, and pattern recognition.. ...
Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4) . This chemokine is released from alpha-granules of activated platelets during platelet aggregation, and promotes blood coagulation by moderating the effects of heparin-like molecules. Due to these roles, it is predicted to play a role in wound repair and inflammation. It is usually found in a complex with proteoglycan. The gene for human PF4 is located on human chromosome 4. Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair. PF4 is ...
Background & Aims: It is more important to know which of physical activities could be the most effective way to cause angiogenesis. In this regards, we have investigated an 8-week aerobic training on vascular endothelial growth factor (VEGF) and Endostatin (ES) in sedentary women. Materials & Methods: 20 volunteer inactive women ...
Dr. Maniscalco studies pulmonary microvascular development in lung injury. Using various animal models, including a non-human primate model of BPD, this work examines the effects of oxygen and ventilation of immature lung on the development of alveolar capillaries. The major goals of the research are to characterize microvascular development in lung injury and investigate angiogenic and angiostatic regulators in normal and injured lung. Recent work has linked expression of inflammatory CXC chemokine mediators, which regulate angiogenesis and are part of the pathophysiology of BPD, to impaired lung microangiogenesis. ...
This issue contains an important contribution from the team of Douglas Lauffenburger, Chair of the Editorial Board, at MIT, USA, in which they demonstrate the ability to characterise quantitative data on phenotypic behaviour of individual endothelial cells in response to angiogenesis signalling and relate it to overall population behaviour. They show that the behaviour of microvascular endothelial cells in a single population is heterogeneous and cannot be assumed to be the same for all cells in a given condition.. Endothelial cell phenotypic behaviors cluster into dynamic state transition programs modulated by angiogenic and angiostatic ...
microRNA-21 (miR-21) is the most commonly upregulated miRNA in solid tumors. This cancer-associated microRNA (oncomiR) regulates various downstream effectors associated with tumor pathogenesis during all stages of carcinogenesis. In this study, we analyzed the function of miR-21 in noncancer cells of the tumor microenvironment to further evaluate its contribution to tumor progression. We report that the expression of miR-21 in cells of the tumor immune infiltrate, and in particular in macrophages, was responsible for promoting tumor growth. Absence of miR-21 expression in tumor- associated macrophages (TAMs), caused a global rewiring of their transcriptional regulatory network that was skewed toward a proinflammatory angiostatic phenotype. This promoted an antitumoral immune response characterized by a macrophage-mediated improvement of cytotoxic T-cell responses through the induction of cytokines and chemokines, including IL-12 and C-X-C motif chemokine 10. These effects translated to a ...
LAA037Ra71, Biotin-Linked Polyclonal Antibody to Fibronectin (FN), 纤连蛋白(FN)多克隆抗体(生物素标记), FN1; CIG; FINC; LETS; MSF; GFND2; Anastellin; Migration-Stimulating Factor; Cold-Insoluble Globulin; Large, External, Transformation-Sensitive Protein | 仅供体外研究使用,不用于临床诊断!请索取进口关税税单及报关单!
RPA037Bo01, Recombinant Fibronectin (FN), 纤连蛋白(FN)重组蛋白, FN1; CIG; FINC; LETS; MSF; GFND2; Anastellin; Migration-Stimulating Factor; Cold-Insoluble Globulin; Large, External, Transformation-Sensitive Protein | 仅供体外研究使用,不用于临床诊断!请索取进口关税税单及报关单!
Treatment of patients with the VEGFR TKIs sorafenib and sunitinib can lead to periods of tumor stability, but resistance to therapy is inevitable. We used a mouse model to define mechanisms by which resistance develops and found that components of the IFNγ signaling pathway are lost with sunitinib or sorafenib therapy. Our data also show that CXCL9 treatment delays resistance to sunitinib in 786-O- and A498-derived tumors and that one mechanism by which this occurs is by prolongation of the antiangiogenic effects of sunitinib. These data suggest that angiostatic pathways are suppressed as a result of VEGFR TKI therapy and set the stage for the subsequent development of resistance to therapy.. In the setting of VEGFR inhibition, RCC tumors undergo extensive necrosis (22). In the setting of this necrosis and accompanying hypoxia/nutrient deprivation, tumors may undergo compensatory changes, including the induction of salvage angiogenesis pathways. We propose that the environmental stress ...
Treatment of metastatic renal cell cancer (RCC) with antiangiogenic agents that block vascular endothelial growth factor (VEGF) receptor 2 signaling produces tumor regression in a substantial fraction of patients; however resistance typically develops within 6-12 months. The purpose of this study was to identify molecular pathways involved in resistance. Treatment of mice bearing either 786-0 or A498 human RCC xenografts with sorafenib or sunitinib produced tumor growth stabilization followed by regrowth despite continued drug administration analogous to the clinical experience. Tumors and plasma were harvested at day 3 of therapy and at the time of resistance to assess pathways that may be involved in resistance. Serial perfusion imaging, and plasma and tumor collections were obtained in mice treated with either placebo or sunitinib alone or in combination with intratumoral injections of the angiostatic chemokine CXCL9. Sunitinib administration led to an early down-modulation of interferon ...
He discovered several angiostatic proteins for potential treatment of cancer. Cao's laboratory discovered catechins in green ...
When mutations occur in the rhodopsin the directional protein movement is affected because the mutations can affect protein ... Many angiostatic factors have been shown to counteract the effect of increasing local VEGF. The naturally occurring form of ... The protein RPE65 is used in the retinoid cycle where the all-trans-retinol within the rod outer segment is isomerized to its ... The opsin proteins are made in the photoreceptor inner segments, then transported to the outer segment, and eventually ...
... matrix protein (M), fusion protein (F), neuraminidase (NA) and large protein (L). All these proteins have variable functions ... regulation of cell growth and mediation of angiostatic effects. Human mast cell infection with SeV induces expression of ... "ASGR1 protein expression summary - The Human Protein Atlas". Retrieved 2020-01-14. "ASGR2 protein ... matrix protein (M), fusion protein (F), hemagglutinin-neuraminidase (HN) and large (L) protein in this order from the 3' ...
... angiostatic proteins MeSH D12.644.276.100.450.500 - angiostatins MeSH D12.644.276.100.450.750 - endostatins MeSH D12.644. ... 14-3-3 proteins MeSH D12.644.360.024.318 - proto-oncogene proteins c-crk MeSH D12.644.360.024.326 - proto-oncogene proteins c- ... ral gtp-binding proteins MeSH D12.644.360.525.462 - ran gtp-binding protein MeSH D12.644.360.525.475 - rap gtp-binding proteins ... wnt proteins MeSH D12.644.276.996.500 - wnt1 protein MeSH D12.644.276.996.750 - wnt2 protein MeSH D12.644.360.011 - activating ...
... angiostatic proteins MeSH D23.348.479.311.200.500 - angiostatins MeSH D23.348.479.311.200.750 - endostatins MeSH D23.348. ... hiv core protein p24 MeSH D23.050.327.520.330 - hiv envelope protein gp41 MeSH D23.050.327.520.350 - hiv envelope protein gp120 ... adenovirus e1 proteins MeSH D23.050.327.045.060 - adenovirus e2 proteins MeSH D23.050.327.045.070 - adenovirus e3 proteins MeSH ... ldl-receptor related protein 2 MeSH D23.050.422.500.750 - ldl-receptor related protein-associated protein MeSH D23.050.550.325 ...
proliferin-related protein. mannose 6-phosphate binding lysosomal protein[10]. One of the most recent methods that is being ... angiostatic steroids + heparin. inhibit basement membrane degradation. Cartilage-Derived Angiogenesis Inhibitory Factor. ... Endogenous inhibitors are often derived from the extracellular matrix or basement membrane proteins and function by interfering ... Kobayashi, H; Matsunaga, K; Oguchi, Y (1995). "Antimetastatic effects of PSK (Krestin), a protein-bound polysaccharide obtained ...
They may use a similar "protein-adduction" mechanism; if so the target protein(s) for these effects have not been defined or ... HPETE is angiostatic and 15(S)-HETE is angiogenic". Inflammation Research. 61 (7): 707-18. doi:10.1007/s00011-012-0463-5. PMID ... 15-hydroxyeicosatetraenoic acid promotes hepatocellular cancer cells growth through protein kinase B and heat shock protein 90 ... Wang, Y; Liang, D; Wang, S; Qiu, Z; Chu, X; Chen, S; Li, L; Nie, X; Zhang, R; Wang, Z; Zhu, D (2010). "Role of the G-protein ...
This is not evidence that MMP-2 and MMP-9 directly cleave perlecan protein in vivo but shows that the proteins clearly modulate ... the angiostatic C-terminal fragment of perlecan". J. Biol. Chem. 280 (8): 7080-7. doi:10.1074/jbc.M409841200. PMID 15591058. ... "Fibroblast growth factor-binding protein is a novel partner for perlecan protein core". J. Biol. Chem. 276 (13): 10263-71. doi: ... Protein Kinase C is activated and perlecan message and protein are subsequently increased. In contrast, the usual ...
Vascular endothelial growth factor (VEGF) is a type of protein the body cells produce to stimulate the growth of new blood ... angiostatic), or lower the permeability of blood vessels (anti-permeability), in turn curing various eye diseases. Disorders/ ... It is shown to be able to significantly decrease intercellular adhesion molecule-1 mRNA and protein levels and therefore reduce ...
"Angiostatic Proteins" by people in Harvard Catalyst Profiles by year, and whether "Angiostatic Proteins" was a major or minor ... "Angiostatic Proteins" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical ... Below are the most recent publications written about "Angiostatic Proteins" by people in Profiles. ... Below are MeSH descriptors whose meaning is more general than "Angiostatic Proteins". ...
Interferon-gamma-inducible protein 10 (IP-10) is an angiostatic factor that inhibits human non-small cell lung cancer (NSCLC) ... We now report that IP-10, a recently described angiostatic factor, as a potent angiostatic factor that regulates non-small cell ... Interferon-gamma-inducible protein 10 (IP-10) is an angiostatic factor that inhibits human non-small cell lung cancer (NSCLC) ... Neovascularization within the tumor is regulated, in part, by a dual and opposing system of angiogenic and angiostatic factors ...
Interferon-γ-inducible protein 10 (IP-10) is an angiostatic factor that inhibits human non-small cell lung cancer (NSCLC) ... and the separated proteins were transferred to nitrocellulose. The blots were probed for the proteins of interest with specific ... Protein concentration was determined by bicinchoninic acid protein assay reagent (Pierce). Lysates were fractionated in either ... Renal Cancer Resistance to Antiangiogenic Therapy Is Delayed by Restoration of Angiostatic Signaling. Rupal S. Bhatt, Xiaoen ...
gal-1 Is a Target Protein for Angiostatic Therapy.. Because gal-1 was initially identified as a receptor for the angiostatic ... Insets) Detail of gal-1 staining in EC (arrow). (D) gal-1 mRNA (qPCR; n = 5) and protein (FACS; n = 4) expression are up- ... gal-1 Binds the Angiostatic Peptide Anginex.. The goal of the present study was to identify the receptor of anginex, an ... 1 C Right). These data demonstrate that the amount of gal-1 protein is up-regulated in angiogenically active EC. Indeed, growth ...
Fusion proteins of the invention include a junction region having an amino acid change that reduces the ability of a junctional ... or modifying one or more amino acids in the junction region of a fusion protein in order to identify a T-cell epitope and ... Disclosed are compositions and methods for producing fusion proteins with reduced immunogenicity. ... Methods of expressing angiostatic protein. US5886178. 28 May 1997. 23 Mar 1999. Syntex (U.S.A.) Inc.. 3-aroylbenzylpyridazinone ...
Boyes J, Bird A (1991) DNA methylation inhibits transcription indirectly via a methyl-CpG binding protein. Cell 64(6):1123-1134 ... Hendrich B, Bird A (1998) Identification and characterization of a family of mammalian methyl-CpG binding proteins. Mol Cell ... Hamming LC, Slotman BJ, Verheul HMW, Thijssen V (2017) The clinical application of angiostatic therapy in combination with ... Peserico A, Simone C (2011) Physical and functional HAT/HDAC interplay regulates protein acetylation balance. J Biomed ...
Methods of expressing angiostatic protein. US5886178. 28 May 1997. 23 Mar 1999. Syntex (U.S.A.) Inc.. 3-aroylbenzylpyridazinone ... Arenberg et al., (1996), "Interferon-gamma-inducible Protein 10 (IP-10) is an Angiostatic Factor that Inhibits Human Non-small ... IL-7 fusion proteins. US7465447. 30 Dic 2004. 16 Dic 2008. Merck Patent Gmbh. Fc-erythropoietin fusion protein with improved ... Once expressed, the proteins of the invention can be harvested by standard protein purification procedures (see, e.g., U.S. Pat ...
Hendrich B, Bird A (1998) Identification and characterization of a family of mammalian methyl-CpG binding proteins. Mol Cell ... Epigenetic approach for angiostatic therapy: promising combinations for cancer treatment. *Robert H. Berndsen1. na1, ... Berndsen, R.H., Abdul, U.K., Weiss, A. et al. Epigenetic approach for angiostatic therapy: promising combinations for cancer ... Boyes J, Bird A (1991) DNA methylation inhibits transcription indirectly via a methyl-CpG binding protein. Cell 64(6):1123-1134 ...
Angiostatic Proteins. Proteins that specifically inhibit the growth of new blood vessels (ANGIOGENESIS, PHYSIOLOGIC). ... Angiogenic Proteins. Intercellular signaling peptides and proteins that regulate the proliferation of new blood vessels under ... This is accomplished by endogenous ANGIOGENIC PROTEINS and a variety of other chemicals and pharmaceutical agents. ... normal physiological conditions (ANGIOGENESIS, PHYSIOLOGICAL). Aberrant expression of angiogenic proteins during disease states ...
He discovered several angiostatic proteins for potential treatment of cancer. Caos laboratory discovered catechins in green ...
It can b...Annemiek Nap a doctor at the University Hospital Maastricht The Neth...They used four angiostatic compounds: anti- ... human vascular endothelial ...,Endometriosis:,Could,angiostatic,therapy,be,the,new,treatment,of,the,future?,biological,biology ... Could a specific protein contribute to erectile dysfunction for the diabetic and obese?. 5. Could diabetes treatments fight ... "We found that angiostatic therapy inhibits the number of newly developed blood vessels around lesions. However, the mature ...
That the full-length protein is inactive is consistent with the angiostatic activity of a TrpRS fragment being part of a ... Angiostatic Activity of TrpRS Fragments in CAM Assay.. Chick CAM assays were performed to test for angiostatic activity of ... 4), whereas human full-length TrpRS had no observable angiostatic activity (data not shown). As a control, the angiostatic CXC- ... Angiostatic activity was evaluated based on the effect of injected proteins on formation of the deep (secondary) retinal ...
Angiostatic Proteins and Peptides Jessica C. A. Bouma-ter Steege, Kevin H. Mayo, Arjan W. Griffioen 16 pages DOI: 10.1615/ ...
This was confirmed by in vitro studies of cultured EC at the protein and mRNA levels. The new angiostatic designer peptide ... This partial peptide mimetic is the first endothelial cell-specific molecule designed as a substitute for an angiostatic ... Early in Vivo Assessment of Angiostatic Therapy Efficacy by Molecular MRI FASEB Journal : Official Publication of the ... Nov, 2003 , Pubmed ID: 12947097 Based on structure-activity relationships of the angiostatic beta-sheet-forming peptide anginex ...
Circulating 38-kDa proteins that are internal peptide fragments of PLASMINOGEN. The name derives from the fact that they are ... Angiostatins contain four KRINGLE DOMAINS which are associated with their potent angiostatic activity. ...
When mutations occur in the rhodopsin the directional protein movement is affected because the mutations can affect protein ... Many angiostatic factors have been shown to counteract the effect of increasing local VEGF. The naturally occurring form of ... The protein RPE65 is used in the retinoid cycle where the all-trans-retinol within the rod outer segment is isomerized to its ... The opsin proteins are made in the photoreceptor inner segments, then transported to the outer segment, and eventually ...
Cloning an artificial gene encoding angiostatic anginex: from designed peptide to functional recombinant protein. Biochem ... B, galectin-1 protein levels in culture medium of F9, B16F10, and TC-1 cells as determined by ELISA. C, tumor growth curves of ... A carbohydrate-binding protein, Galectin-1, promotes proliferation of adult neural stem cells. Proc Natl Acad Sci U S A 2006; ... Endomembrane trafficking of ras: the CAAX motif targets proteins to the ER and Golgi. Cell 1999;98:69-80. ...
In contrast, ELR-negative CXC chemokines (such as IP-10) have angiostatic and antifibrotic properties. Early IP-10 upregulation ... Activated myofibroblasts produce extracellular matrix proteins and an extensive microvascular network is formed. Maturation of ... Evidence suggests that TGF-β induces cardiomyocyte hypertrophy, has complex, context-dependent angiogenic and angiostatic ... has direct anti-inflammatory effects mediated through CD47 and exerts potent angiostatic effects. Thus, TSP-1 induction in the ...
TECs isolated from CRC with an angiostatic Th1-TME and control-TME are pure and show an EC phenotype. ... The gene encoding the matricellular protein SPARCL1 was most strongly upregulated in Th1-TME TECs. It was also highly expressed ... Matricellular protein SPARCL1 regulates tumor microenvironment-dependent endothelial cell heterogeneity in colorectal carcinoma ... Matricellular protein SPARCL1 regulates tumor microenvironment-dependent endothelial cell heterogeneity in colorectal carcinoma ...
Ab116687 is a full length protein produced in Escherichia coli and has been validated in… ... Buy our Recombinant Human Tryptophanyl tRNA synthetase protein. ... Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity ... Recombinant Human Tryptophanyl tRNA synthetase protein. See all Tryptophanyl tRNA synthetase proteins and peptides. ... Proteins and Peptides. Proteomics tools. Agonists, activators, antagonists and inhibitors. Lysates. Multiplex miRNA assays. By ...
Human PDGF-BB protein (220-BB) is manufactured by R&D Systems, over 97% purity. Cited in over 119 publications. Reproducible ... miR-184 exhibits angiostatic properties via regulation of Akt and VEGF signaling pathways Authors: Robert M Lavker. FASEB J., ... as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, ... Animal-Free Recombinant Proteins. Recombinant Human PDGF-BB, Animal-Free Protein AFL220 ...
... proteins and CArG binding factor-A (CBF-A). The Hnrpab proteins comprise numerous proteins with a general packing role in RNA ... Zoeller, J. J., and Iozzo, R. V. (2008 ) Proteomic profiling of endorepellin angiostatic activity on human endothelial cells. ... and protein concentration was measured using the Bio-Rad protein assay. For DIGE minimal labeling, 25 μg of protein sample were ... the analysis of 45 protein spots allowed the identification of 28 different proteins (Table I). Single peptide-based protein ...
Method for purifying fibroblast growth factor protein. US5135920 *. 9 Nov 1989. 4 Ago 1992. Takeda Chemical Industries, Ltd.. ... Angiostatic agents. US5141928 *. 20 Dic 1989. 25 Ago 1992. Lawrence Goldman. Ophthalmic medication. ...
Recombinant protein was administered twice daily in two equal doses of 20 μg i.p. from day +7-13 after transplantation of 5 × ... Others have reported that angiostatic properties of Mig are important to tumor inhibition (16, 17, 18) . To determine whether ... Pertl U., Luster A. D., Varki N. M., Homann D., Gaedicke G., Reisfeld R. A., Lode H. N. IFN-γ-inducible protein-10 is essential ... These studies are consistent with a study by Berman et al. (4) showing therapeutic activity of recombinant protein in models of ...
Removing part or all of this entire domain reveals a protein with angiostatic activity. The NH2-terminal domain, which can be ... A mature protein having a prosequence is a proprotein and is an inactive form of the protein. Once the prosequence is cleaved ... Examples of angiostatic fragments of a tryptophanyl tRNA synthetase include mini-TrpRS, T1, and T2 and any angiostatic ... Thus, for example, the polynucleotide of the present invention can encode for a mature protein, or for a protein having a ...
23 These heterotrimeric G proteins are composed of α (defines the identity of the protein), β, and γ subunit. The chemokine ... ELR− CXC chemokines attract lymphocytes with angiostatic properties.22,23. CXC Chemokine Biology During Early Cardiac Allograft ... IFN-gamma-inducible protein-10 is essential for the generation of a protective tumor-specific CD8 T cell response induced by ... IFN-gamma-inducible protein 10 (IP-10; CXCL10)-deficient mice reveal a role for IP-10 in effector T cell generation and ...
Hall A. G proteins and small GTPases: distant relatives keep in touch. Science. 1998; 280: 2074-2075. ... Both proangiogenic49,50⇓ and angiostatic29 effects of statins have recently been reported. Those opposite effects of statins ... The cholesterol-lowering statins interfere with RhoA activation in ECs.36,48⇓ Thus, the involvement of Rho proteins in ... Activation of RhoA by thrombin in endothelial hyperpermeability: role of Rho kinase and protein tyrosine kinases. Circ Res. ...
Combination angiostatic therapy completely inhibits ocular and tumor angiogenesis. Proc. Natl. Acad. Sci. U. S. A. 104:967-972 ... Monocyte chemoattractant protein-1 mediates retinal detachment-induced photoreceptor apoptosis. Proc. Natl. Acad. Sci. U. S. A. ... Peptides derived from two separate domains of the matrix protein thrombospondin-1 have anti-angiogenic activity. J. Cell Biol. ... Molecular and biological properties of the vascular endothelial growth factor family of proteins. Endocr. Rev. 13:18-32. View ...
CXCL10 / IP10 Recombinant Protein (Active), GTX48407-PRO, Applications: ELISA, WB, Functional Assay; ELISA, Western Blot (WB), ... Additionally, it is angiostatic and mitogenic for vascular smooth muscle cells. Recombinant human IP-10 is an 8.5 kDa protein ... When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. ... gammaInterferon Inducible Protein 10, crg-2, gamma-Interferon Inducible Protein 10, gammaInterferon Inducible Protein10, CXCL10 ...
... and adhesion to extracellular matrix proteins. SPRY1 knockdown was also shown to affect the expression of cyclinD1 and p21 both ... SPRY1 by comparing the transcriptomes of untreated endothelial cells with those of endothelial cells treated by the angiostatic ... Total protein was extracted from these cells and the level of phospho-Erk1/2 protein was measured by Western blotting. The ... SPRY1 protein expression analyzed by Western blotting on total protein extracted from ABAE cells treated for 1 to 6 hours with ...
  • Intercellular signaling peptides and proteins that regulate the proliferation of new blood vessels under normal physiological conditions (ANGIOGENESIS, PHYSIOLOGICAL). (
  • Rabbit polyclonal antibodies to Mig and IP-10 were produced by Biosynthesis, Inc. (Lewisville, TX) using synthetic peptides selected from the IP-10 and Mig protein sequences (CIHIDDGPVRMRAIGK and CISTSRGTIHYKSLKDLKQFAPS, respectively) coupled to carrier protein keyhole limpet hemocyanin. (
  • The chemokine interferon-γ inducible protein 10 kDa (CXCL10) is a member of the CXC chemokine family which binds to the CXCR3 receptor to exert its biological effects. (
  • CXCL10 is involved in chemotaxis, induction of apoptosis, regulation of cell growth and mediation of angiostatic effects. (
  • CXCL10 specifically activates a receptor, CXCR3, which is a seven trans-membrane-spanning G protein-coupled receptor ( 6 ) predominantly expressed on activated T lymphocytes (Th1) ( 7 ), natural killer (NK) cells, inflammatory dentritic cells, macrophages and B cells ( 8 , 9 ). (
  • The ELR + CXC chemokines are potent promoter of angiogenesis, whereas the ELR - chemokine, such as CXCL10, display angiostatic properties (Belperio et al. (
  • 2000). CXCL10 exerts its function through binding to CXCR3 , a seven trans-membrane receptor coupled to G proteins. (
  • CXCL10 is also named 10kDa interferon γ-induced protein (IP-10), as its secretion by CD4+, CD8+, NK and NK-T cells is dependent on IFN-γ , which is itself mediated by the IL-12 cytokine family. (
  • 1985). The CXCL10 cDNA arises from 4 exons encoding for a 12 kDa protein (Liu et al. (
  • Transcription CXCL10, also named IP-10 for Interferon-g-inducible protein 10, is induced by a large range of innate and adaptive immune stimuli that induce the production of IFN type-1 and tye-2. (
  • 2010). CXCL10 mRNA is stabilized by S100b protein binding at the 3'-UTR regions (Shanmugam et al. (
  • The three-dimensional crystal structure of CXCL10 has been determined under different conditions, and the protein structural data have the following accession codes 1lv9, 1o7y, and 1o80 in the Protein Data Bank (Fig. 1). (
  • CXCL10 is a secreted protein present in body fluids and in tissues. (
  • In addition, the production of angiostatic chemokines such as CXCL10 was not affected. (
  • The Human Inflammatory Protein-10 (Hu IP-10/CXCL10) ELISA quantitates Hu IP-10/CXCL10 in human serum, plasma, saliva, buffered solution, or cell culture medium. (
  • CXCL10 ( C-X-C Motif Chemokine Ligand 10) is a 10 kDa protein and member of the CXC family that acts by binding to CXCR3. (
  • CXCL10 exhibits an angiostatic and an antitumor role that is also involved in Th1 inflammatory diseases, Hashimoto's thyroiditis, Graves' disease and Type 1 diabetes mellitus. (
  • We have recently described a marked transient induction of the angiostatic CXC chemokine CXCL10/Interferon-γ inducible Protein (IP)- 10 in the infarct. (
  • Proteins that specifically inhibit the growth of new blood vessels (ANGIOGENESIS, PHYSIOLOGIC). (
  • We now report that IP-10, a recently described angiostatic factor, as a potent angiostatic factor that regulates non-small cell lung cancer (NSCLC)-derived angiogenesis, tumor growth, and spontaneous metastasis. (
  • We describe that galectin-1 (gal-1) is a receptor for the angiogenesis inhibitor anginex, and that the protein is crucial for tumor angiogenesis. (
  • Thus, gal-1 regulates tumor angiogenesis and is a target for angiostatic cancer therapy. (
  • We recently developed the specific angiostatic peptide anginex, which inhibits tumor growth through specific inhibition of angiogenesis ( 4 - 6 ). (
  • Aberrant expression of angiogenic proteins during disease states such as tumorigenesis can also result in PATHOLOGICAL ANGIOGENESIS. (
  • Mini TrpRS is shown here to be angiostatic in a mammalian cell culture system, the chicken embryo, and two independent angiogenesis assays in the mouse. (
  • Thus, protein synthesis may be linked to the regulation of angiogenesis by a natural fragment of TrpRS. (
  • This molecule, bactericidal/permeability-increasing protein (BPI), that was discovered as a bacterial permeabilizing and lipopolysaccharide-neutralizing protein, was found to inhibit angiogenesis by specific induction of apoptosis in endothelial cells. (
  • Because increased expression of the protein has been reported in different human cancers ( 11 - 13 ), we hypothesized that galectin-1 secreted by tumor cells could also stimulate tumor angiogenesis via activation of endothelial cells. (
  • The present invention provides therapeutic methods for treatment of conditions including the neutralization of the anti-coagulant activity of heparin, inhibition of angiogenesis, tumor and endothelial cell proliferation, and treatment of chronic inflammatory diseases by administration of bactericidal/permeability-increasing (BPI) protein products. (
  • The present invention relates to therapeutic uses of bactericidal/permeability increasing (BPI) protein products for the treatment of conditions related to gram-negative bacterial infection and the conditions not directly associated with gram-negative bacterial infection, including neutralization of the anti-coagulant properties of heparin, inhibition of angiogenesis, tumor and endothelial cell proliferation and treatment of chronic inflammatory disease states such as arthritis. (
  • The latter has demonstrated angiostatic effects, and it can mediate inhibition of angiogenesis by associating with altered proangiogenic and antiangiogenic cytokines. (
  • In contrast, when assayed in more complex angiogenesis test systems characterized by the presence of multiple mediators, including in vitro wound-healing (2.5 nmol/L versus 12.5 nmol/L), Matrigel (60 nmol/L versus 300 nmol/L), and chorioallantoic membrane assays, CXCL4L1/PF-4var 47-70 was found to be significantly (5-fold) more angiostatic than CXCL4/PF-4 47-70 . (
  • The cell surface-associated extracellular adherence protein (Eap) mediates adherence of Staphylococcus aureus to host extracellular matrix components and inhibits inflammation, wound healing, and angiogenesis. (
  • Induction of angiogenesis and secretion of MMPs by plasma cells in active disease may play a role in their medullary and extramedullary dissemination, raising the hypothesis that angiostatic/anti-MMP agents may be used for therapy of MM. (
  • Those substances that stop angiogenesis are called angiostatic substances (the Greek word statikos means stand, as in stand still ) (2). (
  • Ankle homogenates were used in angiogenesis assays in vitro and in vivo, and protein levels of cytokines and growth factors were assessed by enzyme-linked immunosorbent assay. (
  • The angiostatic effect occurred despite high levels of vascular endothelial growth factor (VEGF), and was associated with down-regulation of the proangiogenic cytokines IL-18, CXCL16, and CXCL5 and up-regulation of the angiogenesis inhibitor endostatin. (
  • Conclusion In rat AIA, IL-4 reduces synovial tissue vascularization via angiostatic effects, mediates inhibition of angiogenesis via an association with altered pro- and antiangiogenic cytokines, and may inhibit VEGF-mediated angiogenesis and exert its angiostatic role in part via ΑvΒ3 integrin. (
  • Upregulation of angiostatic factors, such as IP-10, in the first few hours following injury may inhibit premature angiogenesis, until the infarct is debrided and appropriate supportive matrix is formed. (
  • Angiostatins contain four KRINGLE DOMAINS which are associated with their potent angiostatic activity. (
  • CXCL4L1/PF-4var was found to be a more potent angiostatic and antitumoral chemokine than CXCL4/PF-4 in different in vitro assays and in vivo models ( 17 , 19 ). (
  • Eap was recently shown to curb acute inflammatory responses, to enhance internalization of the microorganism into eukaryotic cells, to inhibit wound healing, and to function as a potent angiostatic agent ( 1 , 15 , 18 , 33 ). (
  • Chemokines are potent mediators of cell adhesion and migration through their interactions with a family of G protein-coupled receptors (CCR, CXCR, or CX3CR) expressed on leukocytes. (
  • For example, an alternative splice fragment of tryptophanyl-tRNA synthetase (TrpRS) and a similar natural proteolytic fragment are potent angiostatic factors that act through the vascular endothelial-cadherin receptor and Akt signaling pathway. (
  • One of his recent discoveries is a potent angiostatic protein, conveniently named angiostatin (6). (
  • They used four angiostatic compounds: anti-human vascular endothelial growth factor (anti-hVGF), TNP-470, endostatin and anginex. (
  • Moreover, activation of endothelial cells by tumor cell-derived growth signals results in elevated galectin-1 expression ( 2 , 6 , 7 ) and translocation of the protein to the endothelial cell surface ( 2 , 8 ). (
  • Based on all these findings, it has been proposed that targeting endothelial galectin-1 might be a promising angiostatic cancer therapy ( 1 ). (
  • Furthermore, we provide evidence that exogenous galectin-1 specifically promotes endothelial H-Ras signaling toward the Raf/mitogen-activated protein kinase/extracellular signal-regulated kinase (Erk) kinase (Mek)/Erk pathway and that it stimulates endothelial cell migration and proliferation. (
  • We identified SPRY1 by comparing the transcriptomes of untreated endothelial cells with those of endothelial cells treated by the angiostatic agent 16 K prolactin (16 K hPRL). (
  • Partial SPRY1 knockdown by RNA interference protected endothelial cells from apoptosis as well as increased endothelial cell proliferation, migration, capillary network formation, and adhesion to extracellular matrix proteins. (
  • However, little is known about the functional endothelial receptors that transduce "angiostatic" signals. (
  • Retinas of newborn, juvenile, and adult Callithrix jacchus and Macaca fascicularis monkeys and control human retinas were studied to determine the localization of angiostatin relative to III β-tubulin, glial fibrillary acidic protein, vascular endothelial growth factor (VEGF), platelet endothelial cell adhesion molecule-1 (PECAM), and the angiostatin receptor αvβ3-integrin in the foveal, macular, and peripheral retina. (
  • In contrast, CXCR3B induces anti-proliferative and anti-migratory effects, as exemplified by angiostatic effects on endothelial cells. (
  • Representative 2-D protein profiles from vehicle- and endorepellin-treated endothelial cells are depicted with protein separation according to isoelectric point (pI, x-axis) and molecular weight (kDa, y-axis). (
  • Endorepellin evokes an angiostatic stress signaling cascade in endothelial cells. (
  • Interferon-gamma-inducible protein 10 (IP-10) is an angiostatic factor that inhibits human non-small cell lung cancer (NSCLC) tumorigenesis and spontaneous metastases. (
  • Annemiek Nap, a doctor at the University Hospital Maastricht, The Netherlands, told the conference that she and her colleagues tested whether angiostatic therapy (therapy that inhibits the development of blood vessels) could prevent new endometriosis lesions growing and whether it could interfere with the maintenance and growth of existing lesions. (
  • We found that angiostatic therapy inhibits the number of newly developed blood vessels around lesions. (
  • Neovascularization within the tumor is regulated, in part, by a dual and opposing system of angiogenic and angiostatic factors. (
  • This is caused by a number of ANGIOGENIC PROTEINS. (
  • This is accomplished by endogenous ANGIOGENIC PROTEINS and a variety of other chemicals and pharmaceutical agents. (
  • Stimulation by IFN-γ also induces production of the angiostatic chemokines IP-10 and MIG ( 19 , 20 ) and attenuates expression of the angiogenic chemokine IL-8 ( 21 ). (
  • a balance of angiogenic and angiostatic factors probably maintains foveal avascularity throughout life. (
  • For example, VEGF recombinant mouse and rat protein, FLT (VEGFR1) recombinant human protein, FGF basic recombinant human protein, TGFB1 recombinant human protein and PDGF-BB recombinant mouse protein can be employed as angiogenic inducers for research purposes. (
  • Thrombospondin-1 TSP1 is a modular trimeric protein wit several documented functions, including its role as an angiogenic inhibitor. (
  • 01) levels of the angiogenic basic fibroblast growth factor (FGF)-2 in the active MM patients than in nonactive MM and MGUS patients (153 ± 59, 23 ± 17, and 31 ± 18 pg FGF-2/100 μg of protein, respectively). (
  • depends on the local balance between angiostatic and angiogenic factors (5). (
  • Primary tumors produce angiogenic factors in much greater quantities than angiostatic factors. (
  • Production of this fragment is reported to be stimulated by IFN-γ, a cytokine that also stimulates production of angiostatic factors. (
  • Yes, GS-101 Aganirsen is a completed novel molecule which is different from the other, because all the drug available anti-VEGF, they have an anti-VEGF action mean while GS-101 is targeting the overexpression of protein which is regulating the overexpression of the VEGF downstream. (
  • However, recent studies suggest that some VEGF neutralizing proteins are not effective in some patients [ 7 , 8 ]. (
  • Chemokines are small, structurally related proteins which play a significant role in leukocyte trafficking ( 1 ) by producing chemotactic activity in cells expressing corresponding chemokine receptors. (
  • 1,2 By signaling through G protein-coupled chemokine receptors, chemokines govern a variety of cell responses including cell activation and transmigration in leukocytes, as well as in nonhematopoietic cells. (
  • They are classified into four groups depending on the positioning of the conserved cysteines in the NH 2 -terminal part-CXC, CC, CX 3 C, and C chemokine ligands ( 5 )-and they generally bind to G protein-coupled seven-transmembrane receptors (GPCR) designated CXCR or CCR ( 6 , 7 ). (
  • Chemokines, binding their various G protein-coupled receptors, lead the way for leukocytes in health and inflammation. (
  • We investigated this possibility by testing the potential value of restoration and maintenance of angiostatic chemokines in delaying the acquired resistance to VEGFR blockade in murine human tumor xenograft models. (
  • Thus, GD2 is a convenient tumor-specific marker for targeting immune-stimulatory protein domains to tumor cells for the purpose of raising an effective immune response against the tumor cells to destroy them. (
  • While the 14.18 mouse antibody (m14.18 antibody) may assist the targeting of these protein domains to tumor cells, its mouse-derived amino acid sequences can impair the desired therapeutic effect. (
  • Other positive regulators are angiotropin, angiogenin, epidermal growth factor, granulocyte colony-stimulating factor, interleukin-1 (IL-1), IL-6, IL-8, platelet-derived growth factor (PDGF), tumor necrosis factor-α (TNF-α), and matrix proteins such as collagen and the integrins. (
  • zebularine enhances tumor cell chemo- and radiosensitivity and has antimitogenic and angiostatic activities [1] . (
  • This knowledge of the specific angiostatic effects of IL-4 may help optimize target-oriented treatment of inflammatory arthritis. (
  • Essential to most RTK-mediated signaling is the activation of the extracellular-signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) signaling cascade. (
  • Fc gamma R-dependent mitogen-activated protein kinase activation in leukocytes: a common signal transduction event necessary for expression of TNF-alpha and early activation genes. (
  • In a receptor-finding study using a yeast two-hybrid screening approach, we identified galectin-1 (gal-1) as a target protein of anginex. (
  • Methods of the invention involve analyzing, changing, or modifying one or more amino acids in the junction region of a fusion protein in order to identify a T-cell epitope and reduce its ability to interact with a T cell receptor. (
  • Receptor Tyrosine Phosphatase, CD148/DEP-1: An Oligomerization-Sensitive Angiostatic Switch. (
  • Immunoblotting and quantitative RT-PCR were conducted to determine PEDF and peroxisome proliferator activated receptor alpha (PPARalpha) expression at protein and mRNA levels, respectively, in ARPE19 after transfection with miR-21 or a scramble micro RNA (miR-s). (
  • G protein-coupled receptor. (
  • IL-33 is bound by IL-1R Accessory Protein (IL-1RAcP) and IL-1 Receptor-Like 1 (IL-1RL1). (
  • IL-36α, IL-36β, IL-36γ, and IL-36RA are all bound by IL-1R Accessory Protein (IL-1RAcP) and IL-1 Receptor Like 2 (IL-1RL2). (
  • Recently, exogenous administration of the well-known CC-chemokine monocyte chemoattractant protein-1 (MCP-1/CCL2) or a deficiency in its receptor CCR2 have been shown to play a crucial role in monocyte recruitment in the adventitia of growing arteries after vessel occlusion. (
  • CXCR2 is a 7-transmembrane G protein-coupled receptor that mediates chemotaxis during an immune response. (
  • CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. (
  • A purification scheme for the recombinant proteins including removal of the histidine-tag has been established. (
  • Recently, a new class of compounds (angiostatic steroids) have been found to inhibit the formation of new blood vessels (i.e. neovascularization) in the eye. (
  • We had shown previously that expression of the CXC chemokines Mig (monokine induced by IFN-γ) and IP-10 (inducible protein 10) is observed in IL-10 transduced but not neo-vector control tumors. (
  • The interferon-induced angiostatic CXC chemokines, monokine induced by interferon (Mig/CXCL9) and interferon-inducible T-cell chemoattractant (I-TAC/CXCL11), also activate CXCR3. (
  • CXCL11), we found that even though I-TAC was secreted from IFN-γ-activated HSVEC to lower levels than IFN-induced protein-10 or the monokine induced by IFN-γ, it was the principal chemokine responsible for CXCR3 internalization. (
  • This demonstration established a link between protein synthesis and signal transduction. (
  • Peserico A, Simone C (2011) Physical and functional HAT/HDAC interplay regulates protein acetylation balance. (
  • Two-dimensional DIGE with a minimal labeling method coupled to nanoflow liquid chromatography-tandem mass spectrometry was used to detect and identify proteins differentially expressed under PCBs conditions. (
  • Encircled protein spots correspond to the five proteins differentially expressed in response to endorepellin treatment. (
  • The angiostatic natural product fumagillin was synthesized in the group of Erik Sorensen, and Barry Sharpless and colleagues parlayed practical catalytic transformations into diverse libraries of organic compounds. (
  • Additionally, it is angiostatic and mitogenic for vascular smooth muscle cells. (
  • Thermo Fisher Scientific offers interferon-β recombinant rat protein and interferon-γ recombinant mouse protein, but also interleukin-4 recombinant human protein. (
  • GBP-1 was initially shown to be among the most highly induced proteins in human fibroblasts exposed to interferon (IFN)-gamma. (
  • Besides its angiostatic activity, CXCL4/PF-4 is implicated in different biological processes, such as coagulation, promotion of neutrophil cell adhesion to endothelium, and inhibition of megakaryopoiesis and hematopoiesis ( 11 - 13 ). (
  • Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. (
  • The lyophilized recombinant protein is stable for at least 2 years from date of receipt at -20˚C. After reconstitution, it is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. Avoid multiple freeze-thaw cycles. (
  • Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and CHAPS with BSA as a carrier protein. (
  • For further dilution, carrier protein (0.1% BSA or HSA) should be added to avoid loss of bioactivity. (
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). (
  • These proteins include an antibody moiety and a cytokine moiety, and are useful for targeting cytokines to diseased cells, such as cancer cells. (
  • With an average of more than 200 publications each year since its founding, the Institute has earned its research identity in the United States and worldwide for its accomplishments in organic synthesis, antibody catalysis, protein structure, and RNA chemistry. (
  • The Institute continued to build on its strengths in antibody catalysis with discoveries by Steve Mayfield and his group of the expression of antibodies in microalgae and the development by Carlos Barbas and his colleagues of recombinant strategies for designer proteins. (
  • Unlike some of the angiostatic steroids, anecortave acetate appears to be lacking in the pharmacological activities typical of the steroid family (i.e. glucocorticoid, anti-inflammatory, cardiovascular, neurologic, diuretic, etc. (
  • gal-1 Binds the Angiostatic Peptide Anginex. (
  • The anchorless extracellular adherence protein (Eap) of S. aureus , also designated major histocompatibility complex class II analogous protein (Map), selectively recognizes extracellular matrix aggregates but binds promiscuously to monomeric matrix macromolecules ( 16 , 21 , 23 ). (
  • Disclosed are compositions and methods for producing fusion proteins with reduced immunogenicity. (
  • Fusion proteins of the invention include a junction region having an amino acid change that reduces the ability of a junctional epitope to bind to MHC Class II, thereby reducing its interaction with a T-cell. (
  • The present invention relates generally to methods and compositions for making and using modified fusion proteins with reduced or no immunogenicity as therapeutic agents. (
  • More specifically, the invention relates to fusion proteins, made less immunogenic by identifying candidate T-cell epitopes and modifying the amino acid sequence to eliminate such epitopes. (
  • Recently it has become apparent that many fusion proteins with artificial activities are useful as therapeutic proteins. (
  • Another example of a therapeutically useful class of fusion proteins is the immunocytokines. (
  • I have successfully generated recombinant baculoviruses that express the three type 1 repeats in tandem P123 and the third type 1 repeat P3 as histidine-tagged fusion proteins. (
  • It was shown recently that human tyrosyl-tRNA synthetase (TyrRS) can be split into two fragments having distinct cytokine activities, thereby linking protein synthesis to cytokine signaling pathways. (
  • Recently, we demonstrated that one of the tRNA synthetases-human tyrosyl-tRNA synthetase (TyrRS)-has novel cytokine functions in addition to its role in protein synthesis ( 1 ). (
  • The results suggest that PCBs induce mechanisms against oxidative stress (peroxiredoxins 1 and 2), adaptative changes in the energetic metabolism (enolase 1, glycerol-3-phosphate dehydrogenase, and creatine kinase muscle and brain types), and the implication of the unfolded protein response system (glucose-regulated protein, 58 kDa). (
  • Recently, it was found that the variant of CXCL4/PF-4 (CXCL4L1/PF-4var) could exert a more pronounced angiostatic and antitumoral effect than CXCL4/PF-4. (
  • Circulating 38-kDa proteins that are internal peptide fragments of PLASMINOGEN. (
  • The primary translation product corresponds to a protein of 12 kDa that is then limited proteolysed in a protein of 98 amino acids mature protein of 10k Da after release of the signal peptide. (
  • 4 The overexpression of YB-1 in arterial SMCs enhanced CCL5 transcriptional activity, CCL5 mRNA and protein expression, and CCL5-mediated monocyte recruitment. (
  • 01) levels of MMP-2 mRNA and protein when compared with nonactive MM and MGUS patients, whereas MMP-9 expression was similar in all groups. (
  • Many therapeutic proteins are normal human proteins. (
  • Thus, complexes of mammalian tRNA synthetases with seemingly disparate proteins may in general be relevant to understanding how their expanded functions are implemented. (
  • During airway growth, the lung acquires a rich blood supply, which is regulated by a balance of proangiogenic growth factors and angiostatic proteins. (
  • For example, interleukin-2, erythropoietin, and growth hormone are all human proteins that are given to humans who already usually make endogenous levels of these proteins. (
  • Using intrinsic protein fluorescence, it was shown that different conditions of pseudotuberculosis bacteria cultivation (temperature, medium, growth phase) led to the changes in spectral properties of porin fluorescence due to the redistribution of the contributions of tyrosine and different classes of tryptophan residues to the total protein emission. (
  • 2017-2020 Foundation for Research in Science and the Humanities at the University of Zurich (STwF).Topic: The role of the bromodomain proteins in arthritis susceptibility and synovial biology. (
  • Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) is a member of the protein tyrosine phosphatase (PTP) superfamily. (
  • GBP-1 belongs to the class of large GTPases that contains, in addition to the GBPs, three further groups of proteins, which share structural and biochemical properties: the dynamins, the Mx proteins and the atlastins. (
  • Biophysical Studies of the Type 1 Repeats of Human Thrormbospondin-1 to Characterize the Structural Basis of Its Angiostatic Effect. (
  • This study seeks to define the structural basis for the angiostatic effect of the hTSP1 type 1 repeats. (
  • Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. (
  • This cascade is precisely controlled by the activity of various regulatory proteins [ 2 ], including members of the Sprouty (SPRY) protein family. (
  • They not only identified the rat version of LKB1, but also found two proteins that bind to LKB1 and enhance its activity. (
  • Increased MMP2 and MMP9 activity in the ischemic retina has been linked to the proteolytic degradation of pigmented epithelial derived factor (PEDF), a key retinal angiostatic factor. (
  • METHODS: A Phase II trial was initiated to analyze the activity of a continuously administered molecularly targeted treatment regimen (daily pioglitazone [45 mg administered orally] and rofecoxib [25 mg administered orally]) combined with sequentially added angiostatic chemotherapy for patients with previously treated metastatic melanoma (n = 19) or soft tissue sarcoma (n = 21). (
  • In general, immune responses against completely normal human proteins are rare when these proteins are used as therapeutics. (
  • γ-secretase inhibitors are commonly used to probe NOTCH function, but also block processing of numerous other proteins. (
  • The dynamics of protein-protein interactions were unraveled by Jeff Kelly and his group with an eye to discovering small-molecule inhibitors for the prevention of amyloid diseases. (
  • The gene encoding the matricellular protein SPARCL1 was most strongly upregulated in Th1-TME TECs. (
  • Gene therapy represents an attractive alternative to recombinant protein administration for several reasons. (
  • Similarly, in the SLN-CFP reporter, Cyan Fluorescent Protein (CFP) was integrated downstream of the coding region of the atrial-specific gene, Sarcolipin (SLN). (
  • The FOXP1 gene encodes a member of the forkhead box transcription factor family that contains a DNA-binding domain and a protein-protein interaction domain. (
  • DOI: and '(ISWI) protein which is the catalytic component of multiple chromatin-remodeling complexes with functions in nucleosome assembly and gene repression (Tsukiyama et al. (
  • At high doses zebularine induced changes in apoptotic proteins in a cell line specific manner manifested by alteration in caspase-3, Bax, Bcl2 and PARP cleavage [3] . (
  • Induction of directed cell migration by the scratch-wound assay leads to decreased DNaseI sensitivity, alterations in the chromatin binding of architectural proteins and elevated levels of H4K20me1, H3K27me3 and methylated DNA. (
  • 3. The fusion protein of claim 1 , wherein the junction region comprises an IgG sequence having an ATAT amino acid sequence (amino acids 3-6 of SEQ ID NO:6) instead of an LSLS amino acid sequence (amino acids 3-6 of SEQ ID NO:5). (
  • 4. The fusion protein of claim 1 , wherein the junction region comprises an IgG region wherein the LSLS amino acid sequence (amino acids 3-6 of SEQ ID NO:5) is mutated without generating a T-cell epitope. (
  • 7. The fusion protein of claim 1 , wherein the mutation comprises an amino acid substitution to glycine or proline. (
  • Recombinant human IP-10 is an 8.5 kDa protein consisting of 77 amino acids including the four conserved cysteine residues present in CXC chemokines. (
  • Since amino acids are the prime building blocks of all cellular proteins, destruction of these molecules has a devastating achieve on the microorganism. (
  • Residues 12-65 of the NH 2 -terminal extension of human TrpRS are homologous to the WHEP-TRP conserved domain protein family (pfam00458) ( 8 ). (
  • Proteomics has been initially used successfully in drug discovery, biomarker identification, and protein-protein interaction studies in human disease processes ( 23 , 24 ). (
  • Subsequently, the feasibility of an eap -based approach was systematically analyzed on the genomic, transcriptional, and protein levels by use of a large collection of human- and veterinary-derived S. aureus and non- S. aureus staphylococcal isolates. (
  • The endosperm varies in ploidy and contains reserves of starch, oils, and proteins, making it an important source of human nutrition. (
  • Angiostatic Proteins" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (