An angiopoietin that is closely related to ANGIOPOIETIN-1. It binds to the TIE-2 RECEPTOR without receptor stimulation and antagonizes the effect of ANGIOPOIETIN-1. However its antagonistic effect may be limited to cell receptors that occur within the vasculature. Angiopoietin-2 may therefore play a role in down-regulation of BLOOD VESSEL branching and sprouting.
The first to be discovered member of the angiopoietin family. It may play a role in increasing the sprouting and branching of BLOOD VESSELS. Angiopoietin-1 specifically binds to and stimulates the TIE-2 RECEPTOR. Several isoforms of angiopoietin-1 occur due to ALTERNATIVE SPLICING of its mRNA.
A TIE receptor tyrosine kinase that is found almost exclusively on ENDOTHELIAL CELLS. It is required for both normal embryonic vascular development (NEOVASCULARIZATION, PHYSIOLOGIC) and tumor angiogenesis (NEOVASCULARIZATION, PATHOLOGIC).
A family of structurally-related angiogenic proteins of approximately 70 kDa in size. They have high specificity for members of the TIE RECEPTOR FAMILY.
A TIE receptor found predominantly on ENDOTHELIAL CELLS. It is considered essential for vascular development and can form a heterodimer with the TIE-2 RECEPTOR. The TIE-1 receptor may play a role in regulating BLOOD VESSEL stability and maturation.
A family of structurally-related tyrosine kinase receptors that are expressed predominantly in ENDOTHELIAL CELLS and are essential for development of BLOOD VESSELS (NEOVASCULARIZATION, PHYSIOLOGIC). The name derives from the fact that they are tyrosine kinases that contain Ig and EGF domains.
Agents that induce or stimulate PHYSIOLOGIC ANGIOGENESIS or PATHOLOGIC ANGIOGENESIS.
The original member of the family of endothelial cell growth factors referred to as VASCULAR ENDOTHELIAL GROWTH FACTORS. Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as "tumor angiogenesis factor" and "vascular permeability factor". Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability it may play a role in stimulating VASODILATION via NITRIC OXIDE-dependent pathways. Alternative splicing of the mRNA for vascular endothelial growth factor A results in several isoforms of the protein being produced.
The development of new BLOOD VESSELS during the restoration of BLOOD CIRCULATION during the healing process.
Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.
A pathologic process consisting of the proliferation of blood vessels in abnormal tissues or in abnormal positions.
A family of angiogenic proteins that are closely-related to VASCULAR ENDOTHELIAL GROWTH FACTOR A. They play an important role in the growth and differentiation of vascular as well as lymphatic endothelial cells.
A species of gram-negative bacteria highly pathogenic to RATS and MICE. It is the primary cause of murine respiratory mycoplasmosis.
Intercellular signaling peptides and proteins that regulate the proliferation of new blood vessels under normal physiological conditions (ANGIOGENESIS, PHYSIOLOGICAL). Aberrant expression of angiogenic proteins during disease states such as tumorigenesis can also result in PATHOLOGICAL ANGIOGENESIS.
These growth factors are soluble mitogens secreted by a variety of organs. The factors are a mixture of two single chain polypeptides which have affinity to heparin. Their molecular weight are organ and species dependent. They have mitogenic and chemotactic effects and can stimulate endothelial cells to grow and synthesize DNA. The factors are related to both the basic and acidic FIBROBLAST GROWTH FACTORS but have different amino acid sequences.
A class of cellular receptors that have an intrinsic PROTEIN-TYROSINE KINASE activity.
Soluble protein factors generated by activated lymphocytes that affect other cells, primarily those involved in cellular immunity.
Any of the tubular vessels conveying the blood (arteries, arterioles, capillaries, venules, and veins).
A 200-230-kDa tyrosine kinase receptor for vascular endothelial growth factors found primarily in endothelial and hematopoietic cells and their precursors. VEGFR-2 is important for vascular and hematopoietic development, and mediates almost all endothelial cell responses to VEGF.
Unique slender cells with multiple processes extending along the capillary vessel axis and encircling the vascular wall, also called mural cells. Pericytes are imbedded in the BASEMENT MEMBRANE shared with the ENDOTHELIAL CELLS of the vessel. Pericytes are important in maintaining vessel integrity, angiogenesis, and vascular remodeling.
Formation of new blood vessels originating from the retinal veins and extending along the inner (vitreal) surface of the retina.
Glycoproteins found on the membrane or surface of cells.
The minute vessels that connect the arterioles and venules.
Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.
A 180-kDa VEGF receptor found primarily in endothelial cells that is essential for vasculogenesis and vascular maintenance. It is also known as Flt-1 (fms-like tyrosine kinase receptor-1). A soluble, alternatively spliced isoform of the receptor may serve as a binding protein that regulates the availability of various ligands for VEGF receptor binding and signal transduction.
Regulatory proteins and peptides that are signaling molecules involved in the process of PARACRINE COMMUNICATION. They are generally considered factors that are expressed by one cell and are responded to by receptors on another nearby cell. They are distinguished from HORMONES in that their actions are local rather than distal.
The blood vessels which supply and drain the RETINA.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Agents and endogenous substances that antagonize or inhibit the development of new blood vessels.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
A family of closely related RECEPTOR PROTEIN-TYROSINE KINASES that bind vascular endothelial growth factors. They share a cluster of seven extracellular Ig-like domains which are important for ligand binding. They are highly expressed in vascular endothelial cells and are critical for the physiological and pathological growth, development and maintenance of blood and lymphatic vessels.
A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.
Perforations through the whole thickness of the retina including the macula as the result of inflammation, trauma, degeneration, etc. The concept includes retinal breaks, tears, dialyses, and holes.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
The property of blood capillary ENDOTHELIUM that allows for the selective exchange of substances between the blood and surrounding tissues and through membranous barriers such as the BLOOD-AIR BARRIER; BLOOD-AQUEOUS BARRIER; BLOOD-BRAIN BARRIER; BLOOD-NERVE BARRIER; BLOOD-RETINAL BARRIER; and BLOOD-TESTIS BARRIER. Small lipid-soluble molecules such as carbon dioxide and oxygen move freely by diffusion. Water and water-soluble molecules cannot pass through the endothelial walls and are dependent on microscopic pores. These pores show narrow areas (TIGHT JUNCTIONS) which may limit large molecule movement.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
An area approximately 1.5 millimeters in diameter within the macula lutea where the retina thins out greatly because of the oblique shifting of all layers except the pigment epithelium layer. It includes the sloping walls of the fovea (clivus) and contains a few rods in its periphery. In its center (foveola) are the cones most adapted to yield high visual acuity, each cone being connected to only one ganglion cell. (Cline et al., Dictionary of Visual Science, 4th ed)
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Disease of the RETINA as a complication of DIABETES MELLITUS. It is characterized by the progressive microvascular complications, such as ANEURYSM, interretinal EDEMA, and intraocular PATHOLOGIC NEOVASCULARIZATION.
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Cell surface receptors that bind growth or trophic factors with high affinity, triggering intracellular responses which influence the growth, differentiation, or survival of cells.
Venous vessels in the umbilical cord. They carry oxygenated, nutrient-rich blood from the mother to the FETUS via the PLACENTA. In humans, there is normally one umbilical vein.
Removal of the whole or part of the vitreous body in treating endophthalmitis, diabetic retinopathy, retinal detachment, intraocular foreign bodies, and some types of glaucoma.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
The transparent, semigelatinous substance that fills the cavity behind the CRYSTALLINE LENS of the EYE and in front of the RETINA. It is contained in a thin hyaloid membrane and forms about four fifths of the optic globe.
Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Fluid accumulation in the outer layer of the MACULA LUTEA that results from intraocular or systemic insults. It may develop in a diffuse pattern where the macula appears thickened or it may acquire the characteristic petaloid appearance referred to as cystoid macular edema. Although macular edema may be associated with various underlying conditions, it is most commonly seen following intraocular surgery, venous occlusive disease, DIABETIC RETINOPATHY, and posterior segment inflammatory disease. (From Survey of Ophthalmology 2004; 49(5) 470-90)
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.
A hypoperfusion of the BLOOD through an organ or tissue caused by a PATHOLOGIC CONSTRICTION or obstruction of its BLOOD VESSELS, or an absence of BLOOD CIRCULATION.
Elements of limited time intervals, contributing to particular results or situations.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
A single-chain polypeptide growth factor that plays a significant role in the process of WOUND HEALING and is a potent inducer of PHYSIOLOGIC ANGIOGENESIS. Several different forms of the human protein exist ranging from 18-24 kDa in size due to the use of alternative start sites within the fgf-2 gene. It has a 55 percent amino acid residue identity to FIBROBLAST GROWTH FACTOR 1 and has potent heparin-binding activity. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor (FIBROBLAST GROWTH FACTOR 1).
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
The relationship between the dose of an administered drug and the response of the organism to the drug.
The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
A cell line derived from cultured tumor cells.
Established cell cultures that have the potential to propagate indefinitely.

Molecular cloning and characterization of a novel angiopoietin family protein, angiopoietin-3. (1/589)

Using homology-based PCR, we have isolated cDNA encoding a novel member (491 amino acids) of the angiopoietin (Ang) family from human adult heart cDNA and have designated it angiopoietin-3 (Ang3). The NH2-terminal and COOH-terminal portions of Ang-3 contain the characteristic coiled-coil domain and fibrinogen-like domain that are conserved in other known Angs. Ang3 has a highly hydrophobic region at the N-terminus (approximately 21 amino acids) that is typical of a signal sequence for protein secretion. Ang3 mRNA is most abundant in adrenal gland, placenta, thyroid gland, heart and small intestine in human adult tissues. Additionally, Ang3 is a secretory protein, but is not a mitogen in endothelial cells.  (+info)

Angiopoietins 3 and 4: diverging gene counterparts in mice and humans. (2/589)

The angiopoietins have recently joined the members of the vascular endothelial growth factor family as the only known growth factors largely specific for vascular endothelium. The angiopoietins include a naturally occurring agonist, angiopoietin-1, as well as a naturally occurring antagonist, angiopoietin-2, both of which act by means of the Tie2 receptor. We now report our attempts to use homology-based cloning approaches to identify new members of the angiopoietin family. These efforts have led to the identification of two new angiopoietins, angiopoietin-3 in mouse and angiopoietin-4 in human; we have also identified several more distantly related sequences that do not seem to be true angiopoietins, in that they do not bind to the Tie receptors. Although angiopoietin-3 and angiopoietin-4 are strikingly more structurally diverged from each other than are the mouse and human versions of angiopoietin-1 and angiopoietin-2, they appear to represent the mouse and human counterparts of the same gene locus, as revealed in our chromosomal localization studies of all of the angiopoietins in mouse and human. The structural divergence of angiopoietin-3 and angiopoietin-4 appears to underlie diverging functions of these counterparts. Angiopoietin-3 and angiopoietin-4 have very different distributions in their respective species, and angiopoietin-3 appears to act as an antagonist, whereas angiopoietin-4 appears to function as an agonist.  (+info)

Angiopoietin-1 is an apoptosis survival factor for endothelial cells. (3/589)

We examined the effect of angiopoietin-1 (Ang1) on apoptosis in human umbilical vein endothelial cells (HUVECs). Ang1 (5-1000 ng/ml) dose-dependently inhibited apoptosis under a serum-deprived state. A significant apoptotic inhibition occurred with as low as 50 ng/ml. Two hundred ng/ml of Ang1 inhibited to approximately 50% of the control apoptotic rates for 96 h. Furthermore, an augmented antiapoptotic effect of Ang1 by the addition of 20 ng/ml vascular endothelial growth factor was observed. This Ang1-induced strong antiapoptotic effect in endothelial cells is a novel and intriguing finding and could be an additional description of Ang1-induced direct biological function.  (+info)

Vessel cooption, regression, and growth in tumors mediated by angiopoietins and VEGF. (4/589)

In contrast with the prevailing view that most tumors and metastases begin as avascular masses, evidence is presented here that a subset of tumors instead initially grows by coopting existing host vessels. This coopted host vasculature does not immediately undergo angiogenesis to support the tumor but instead regresses, leading to a secondarily avascular tumor and massive tumor cell loss. Ultimately, however, the remaining tumor is rescued by robust angiogenesis at the tumor margin. The expression patterns of the angiogenic antagonist angiopoietin-2 and of pro-angiogenic vascular endothelial growth factor (VEGF) suggest that these proteins may be critical regulators of this balance between vascular regression and growth.  (+info)

The transcription factor MEF2C-null mouse exhibits complex vascular malformations and reduced cardiac expression of angiopoietin 1 and VEGF. (5/589)

The MEF2 family of transcription factors has been implicated in transcriptional regulation in a number of different cell types. Targeted deletion of the MEF2C gene, in particular, revealed its importance for early cardiogenesis (Q. Lin et al., 1997, Science 276, 1404-1407). We report here that this deletion also resulted in vascular anomalies characterized by extreme variability in lumen size and defects in remodeling. While primary vascular networks formed in the yolk sac of the mutants, they failed to remodel into more complex vascular structures. Likewise, although the primordia of the dorsal aortae formed normally, anomalies were observed in these vessels later in development. Dorsal and anterior to the heart, the aortae exhibited abnormally small lumens, as did the anterior cardinal veins and intersegmental arteries. In contrast, the dorsal aortae and intersegmental arteries caudal to the heart were grossly enlarged. Cranial vessels were also enlarged and less branched than normal. Endocardiogenesis in the mutant was abnormal with the endothelial cells exhibiting a number of aberrant phenotypes. These endocardial defects were accompanied by a notable reduction in angiopoietin 1 and VEGF mRNA production by the myocardium, indicating that MEF2C is required for myocardial expression of these important endothelial-directed cytokines and thus for correct endocardial morphogenesis.  (+info)

Expressions of angiopoietins and Tie2 in human choroidal neovascular membranes. (6/589)

PURPOSE: To elucidate the potential role of angiopoietins and the Tie2 system in choroidal neovascularization. METHODS: Surgically excised choroidal neovascular membranes (CNVMs) were obtained at vitrectomy from five eyes with age-related macular degeneration, three eyes with idiopathic neovascular maculopathy, and two eyes had degenerative myopia and two eyes had angioid streaks. Light microscopic immunohistochemistry was performed to detect cytokines such as vascular endothelial growth factor (VEGF), Ang1, and Ang2 and cellular components such as retinal pigment epithelial (RPE) cells, macrophages, and endothelial cells. Immunofluorescent double staining using confocal microscopy was performed to identify the cell types that secrete specific cytokines. RESULTS: Ang1 and Ang2 were positive in all surgically excised CNVMs, regardless of the primary disease. Double staining revealed that many of the cytokeratin, CD68 and factor VIII positive cells also had Ang1 and Ang2 immunoreactivities. In contrast to Ang1, Ang2 immunoreactivity tends to be higher in the highly vascularized regions of many CNVMs, and the localization was very similar to that of VEGF staining. Almost all vascular structures had prominent immunoreactivity for Tie2, which was confirmed by double staining for Tie2 and factor VIII. Tie2 immunoreactivity was also observed in the RPE monolayer and in pigmented, polygonal, and fibroblast-like cells in the stroma. CONCLUSIONS: Present findings that Ang2 and VEGF are co-upregulated and that Tie2 is expressed in a variety of cell types in CNVMs further support a crucial role of the interaction between VEGF and Ang2 in pathologic angiogenesis of CNVM formation.  (+info)

Expression of angiopoietin-1, angiopoietin-2, and the Tie-2 receptor tyrosine kinase during mouse kidney maturation. (7/589)

The Tie-2 receptor tyrosine kinase transduces embryonic endothelial differentiation, with Angiopoietin-1 (Ang-1) acting as a stimulatory ligand and Ang-2 postulated to be a naturally occurring inhibitor. Expression of these genes was sought during mouse kidney maturation from the onset of glomerulogenesis (embryonic day 14 [E14]) to the end of nephron formation (2 wk postnatal [P2]), and during medullary maturation into adulthood (P8). Using Northern and slot blotting of RNA extracted from whole organs, these three genes were expressed throughout the experimental period with peak levels at P2 to P3. By in situ hybridization analysis at E18, P1, and P3, Ang-1 mRNA was found to localize to condensing renal mesenchymal cells, proximal tubules, and glomeruli in addition to maturing tubules of the outer medulla. In contrast, Ang-2 transcripts were more spatially restricted, being detected only in differentiating outer medullary tubules and the vasa recta bundle area. Using in situ hybridization and immunohistochemistry, Tie-2 was detected in capillaries of the nephrogenic cortex, glomerular tufts, cortical interstitium, and medulla including vessels in the vasa recta. Using Western blotting of protein extracted from whole organs, Tie-2 protein was detected between E14 and P8 with tyrosine phosphorylated Tie-2 evident from E18. These data are consistent with the hypothesis that Tie-2 has roles in maturation of both glomeruli and vasa rectae.  (+info)

New model of tumor angiogenesis: dynamic balance between vessel regression and growth mediated by angiopoietins and VEGF. (8/589)

Our analyses in several different tumor settings challenge the prevailing view that malignancies and metastases generally initiate as avascular masses that only belatedly induce vascular support. Instead, we find that malignant cells rapidly co-opt existing host vessels to form an initially well-vascularized tumor mass. Paradoxically, the co-opted vasculature does not undergo angiogenesis to support the growing tumor, but instead regresses (perhaps as part of a normal host defense mechanism) via a process that involves disruption of endothelial cell/smooth muscle cell interactions and endothelial cell apoptosis. This vessel regression in turn results in necrosis within the central part of the tumor. However, robust angiogenesis is initiated at the tumor margin, rescuing the surviving tumor and supporting further growth. The expression patterns of Angiopoietin-2 (the natural antagonist for the angiogenic Tie2 receptor) and vascular endothelial growth factor (VEGF) strongly implicate these factors in the above processes. Angiopoietin-2 is highly induced in co-opted vessels, prior to VEGF induction in the adjacent tumor cells, providing perhaps the earliest marker of tumor vasculature and apparently marking the co-opted vessels for regression. Subsequently, VEGF upregulation coincident with Angiopoietin-2 expression at the tumor periphery is associated with robust angiogenesis. Thus, in tumors, Angiopoietin-2 and VEGF seem to reprise the roles they play during vascular remodeling in normal tissues, acting to regulate the previously underappreciated balance between vascular regression and growth.  (+info)

To test the hypothesis that the concentration of angiopoietin-2 relative to angiopoietin-1 may be a useful biological marker of mortality in acute lung injury patients. We also tested the association of concentration of angiopoietin-2 relative to ang
Angiopoietin-1/Tek signaling is a critical regulator of blood vessel development, with conventional knockout of angiopoietin-1 or Tek in mice being embryonically lethal due to vascular defects. In addition, angiopoietin-1 is thought to be required for the stability of mature vessels. Using a Cre-Lox conditional gene targeting approach, we have studied the role of angiopoietin-1 in embryonic and adult vasculature. We report here that angiopoietin-1 is critical for regulating both the number and diameter of developing vessels but is not required for pericyte recruitment. Cardiac-specific knockout of angiopoietin-1 reproduced the phenotype of the conventional knockout, demonstrating that the early vascular abnormalities arise from flow-dependent defects. Strikingly, deletion in the entire embryo after day E13.5 produced no immediate vascular phenotype. However, when combined with injury or microvascular stress, angiopoietin-1 deficiency resulted in profound organ damage, accelerated angiogenesis, ...
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TY - JOUR. T1 - Systemic analysis of tyrosine phosphorylated proteins in angiopoietin-1 induced signaling pathway of endothelial cells. AU - Kim, Young-Mee. AU - Seo, Jawon. AU - Yung, Hee Kim. AU - Jeong, Jaeho. AU - Hye, Joon Joo. AU - Lee, Dong Hee. AU - Gou, Young Koh. AU - Lee, Kong Joo. PY - 2007/8/1. Y1 - 2007/8/1. N2 - Angiogenesis is an essential process in physiological and pathological processes and is well-regulated to maintain the cellular homeostasis by balancing the endothelial cells in proliferation and apoptosis. Angiopoietin-1 (Ang1) regulates angiogenesis as a ligand of Tie 2 receptor tyrosine kinase. However, the regulation pathways are not well-understood. To date, only a few of the signaling molecules involved in the Tie 2 receptor tyrosine kinase-mediated angiogenesis have been identified. In this study, we systematically identified tyrosine-phosphorylated proteins in Ang1-induced signaling cascade in human umbilical vein endothelial cells (HUVECs), employing proteomic ...
TY - JOUR. T1 - Angiopoietin-1 protects endothelial cells from hypoxia-induced apoptosis via inhibition of phosphatase and tensin homologue deleted from chromosome ten. AU - Lee, Sae Won. AU - Youn, Seock Won. AU - Kim, Tae Youn. AU - Suh, Jung Won. AU - Koh, Gou Young. AU - Kwon, Yoo Wook. AU - Chae, In Ho. AU - Park, Young Bae. AU - Kim, Hyo Soo. PY - 2009/2. Y1 - 2009/2. N2 - Background and Objectives: Angiopoietin-1 (Ang1) is a regulator of blood vessel growth and maturation, and prevents radiation-induced or serum deprivation-induced apoptosis. Phosphatase and tensin homologue deleted from chromosome ten (PTEN), a well-known tumor suppressor, regulates cell cycle arrest and apoptosis. Hypoxia induces apoptosis by increasing the expression of PTEN. We hypothesized that Angl may regulate PTEN expression and, thus, reduce endothelial apoptosis under hypoxia in vitro and in vivo. Materials and Methods: In vitro, human umbilical vein endothelial cells (HUVECs) were treated with Ang1, and ...
The angiopoietin/Tie (ANG/Tie) receptor system controls developmental and tumor angiogenesis, inflammatory vascular remodeling, and vessel leakage. ANG1 is a Tie2 agonist that promotes vascular stabilization in inflammation and sepsis, whereas ANG2 is a context-dependent Tie2 agonist or antagonist. A limited understanding of ANG signaling mechanisms and the orphan receptor Tie1 has hindered development of ANG/Tie-targeted therapeutics. Here, we determined that both ANG1 and ANG2 binding to Tie2 increases Tie1-Tie2 interactions in a β1 integrin-dependent manner and that Tie1 regulates ANG-induced Tie2 trafficking in endothelial cells. Endothelial Tie1 was essential for the agonist activity of ANG1 and autocrine ANG2. Deletion of endothelial ...
Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning. The anticoagulation factor protein S and its relative, Gas6, are ligands for the Tyro 3/Axl family of receptor tyrosine kinases
TY - JOUR. T1 - Angiopoietin-2, permeability oedema, occurrence and severity of ALI/ARDS in septic and non-septic critically ill patients. AU - van der Heijden, M.. AU - van Nieuw Amerongen, G.P.. AU - Koolwijk, P.. AU - van Hinsbergh, V.W.M.. AU - Groeneveld, A.B.J.. PY - 2008. Y1 - 2008. U2 - 10.1136/thx.2007.087387. DO - 10.1136/thx.2007.087387. M3 - Article. C2 - 18559364. VL - 63. SP - 903. EP - 909. JO - Thorax. JF - Thorax. SN - 0040-6376. IS - 10. ER - ...
Effects of Protein and Gene Transfer of the Angiopoietin-1 Fibrinogen-like Receptor-binding Domain on Endothelial and Vessel ...
The results of the present study indicate that TAK1 acts as an AMPKα1 kinase in endothelial cells and positively regulates VEGF-induced, TNF-α-induced, and IL-1β-induced angiogenesis by increasing the expression of the antioxidative mitochondrial enzyme, SOD2. Indeed, superoxide production and the mitochondrial membrane potential were reduced in the absence of TAK1, and the impaired endothelial cell sprouting from aortic rings from TAKΔEC and AMPKα1ΔEC mice was rescued in the presence of PEG-SOD.. The global deletion of TAK1 impairs embryonic development in C. elegans,23 zebrafish, and mice.13 Both the global and endothelial-specific deletions of TAK1 result in vascular malformation, dilated capillaries, and defects in vascular stabilization,14 and correlate well with the phenotype of endothelial cell-specific TGF-β type II receptor conditional mutants.24 In addition to its role in vascular development, TAK1 has also been implicated in the TGF-β-induced regulation of matrix ...
In the circulatory system, while larger features of the vasculature such as arteries and veins appear to be statically positioned for years of use, finer features in the human body and in biology relates to a continuous branching out of a circulatory network that is required for tissue growth. This process is related to biochemical pathways and is vital to be understood so that issues in health and disease can be better addressed. Angiogenesis is the formation of new blood vessels from existing vessels. A functional vascular network is established by balanced angiogenesis processes. While the activation of angiogenesis extends vascular networks crucial for tissue growth, angiogenic vessels should be stabilized to undertake necessary vascular functions. The Notch pathway is fundamental for allowing for vascular stabilization. It acts by preventing excessive angiogenesis. However, how this signaling pathway that is conserved in various biological processes can induce changes that are ...
In this study we found that: (1) LS inhibits tubule formation in HUVECs and HMECs, but not BAECs, however CM from laminar sheared BAECs can inhibit tubule formation of HUVECs, (2) Preconditioning with LS inhibits migration in HUVECs and BAECs, whereas OS does not inhibit migration and this is mediated through secreted protein, (3) Ang2 is downregulated by LS both at the gene and protein level in vitro, (4) Ang2 is downregulated at sites of LS and upregulated at sites of OS in vivo, (5) Knockdown of Ang2 inhibits OS-mediated tubule formation and migration, and (6) Addition of recombinant Ang2 partially rescues LS-inhibited tubule formation. Collectively, these findings suggest that Ang2 secreted by ECs in response to OS, acts as a promigratory and proangiogenic molecule that could play an important role in diseases with altered shear stress.. Fluid shear stress is thought to play a role in angiogenesis and arteriogenesis. In angiogenesis, endothelial proliferation and migration are 2 important ...
The interaction between fluid status and angiopoietin-2 in adverse renal outcomes of chronic kidney disease. Yi-Chun Tsai, Yi-Wen Chiu, Hung-Tien Kuo, Jia-Jung Lee, Su-Chu Lee, Tzu-Hui Chen, Ming-Yen Lin, Shang-Jyh Hwang, Mei-Chuan Kuo, Ya-Ling Hsu, Hung-Chun Chen. Journal article , Research Article , Published 23 Mar 2017 , PLOS ONE ...
Looking for online definition of angiopoietin-2a in the Medical Dictionary? angiopoietin-2a explanation free. What is angiopoietin-2a? Meaning of angiopoietin-2a medical term. What does angiopoietin-2a mean?
TY - JOUR. T1 - Angiopoietin-1 regulates brain endothelial permeability through PTPN-2 mediated tyrosine dephosphorylation of occludin. AU - Siddiqui, M. Rizwan. AU - Mayanil, Chandra S.. AU - Kim, Kwang Sik. AU - Tomita, Tadanori. PY - 2015/6/19. Y1 - 2015/6/19. N2 - Objective Blood brain barrier (BBB) breakdown and increased endothelial permeability is a hallmark of neuro-vascular inflammation. Angiopoietin-1 (Ang-1), a Tie-2 receptor agonist ligand, is known to modulate barrier function of endothelial cells; however the molecular mechanisms related to Ang-1 mediated repair of Tight Junctions (TJs) in brain endothelium still remain elusive. In this study, we investigated a novel role of non-receptor protein tyrosine phosphatase N-2 (PTPN-2) in Ang-1 mediated stabilization of tight junction proteins. Method and Result To study the barrier protective mechanism of Ang-1, we challenged human brain microvascular endothelial cells in-vitro, with a potent inflammatory mediator thrombin. By using ...
Angiopoietins are a family of growth factors that are ligands for the tyrosine kinase receptor, Tie2. Angiopoietin 1 (Ang-1) is agonistic for Tie2, plays a key role in blood vessel maturation and stability and has been shown to possess anti-inflammatory properties. However, Tie2 expression has been demonstrated on human neutrophils and the observation that neutrophils migrate in response to Ang-1 in vitro has confounded research into its exact role in inflammation as well as its potential use as a therapeutic agent. We used a mouse model of peritoneal neutrophilic inflammation to determine if Ang-1 could stimulate neutrophil migration in vivo. Tie2 expression was demonstrated on mouse neutrophils. In addition, recombinant human Ang-1 induced significant chemotaxis of isolated mouse neutrophils in a Tie2- and CD18-dependent manner. Subsequently, co-immunoprecipitation of Ang-1 and CD18 demonstrated their interaction. Intraperitoneal injection of an engineered angiopoietin-1, MAT.Ang-1, induced ...
TY - JOUR. T1 - Coadministration of adenoviral vascular endothelial growth factor and angiopoietin-1 enhances vascularization and reduces ventricular remodeling in the infarcted myocardium of type 1 diabetic rats. AU - Mathews Samuel, Samson. AU - Akita, Yuzo. AU - Paul, Debayon. AU - Thirunavukkarasu, Mahesh. AU - Zhan, Lijun. AU - Sudhakaran, Perumana R.. AU - Li, Chuanfu. AU - Maulik, Nilanjana. PY - 2010/1/1. Y1 - 2010/1/1. N2 - OBJECTIVE - Hyperglycemia impairs angiogenesis in response to ischemia, leading to ventricular remodeling. Although the effects of overexpressing angiogenic growth factors have been studied in inducing angiogenesis, the formation of functional vessels remains a challenge. The present study evaluates the reversal of diabetes-mediated impairment of angiogenesis in the infarcted diabetic rat myocardium by proangiogenic gene therapy. RESEARCH DESIGN AND METHODS - Ad.VEGF and Ad.Ang1 were intramyocardially administered in combination immediately after myocardial ...
Angiopoietin-1 (Ang1) and vascular endothelial growth factor (VEGF) play complementary roles in vascular development during embryogenesis. In the adult, VEGF signaling increases vascular permeability and Ang1 blocks this VEGF-mediated response. Gavard et al. provide evidence that this function of Ang1 is mediated by its actions on the endothelial cells, preventing them from responding to VEGF signaling that triggers disruption of adherens junctions. When injected subcutaneously in mice, Ang1 prevented dermal vascular permeability in response to VEGF injection (acute in vivo vascular permeability model). Experiments with cultured mouse endothelial cells revealed that Ang1 did not prevent VEGF from stimulating downstream effectors involved in proliferation (phosphorylation of Akt, extracellular signal-regulated kinases 1 and 2, and focal adhesion kinase) but did prevent disruption of endothelial barrier function by VEGF. Preexposure of endothelial cell cultures to Ang1 inhibited the ability of ...
TY - JOUR. T1 - Methylglyoxal modification of mSin3A links glycolysis to angiopoietin-2 transcription. AU - Yao, Dachun. AU - Taguchi, Tetsuya. AU - Matsumura, Takeshi. AU - Pestell, Richard. AU - Edelstein, Diane. AU - Giardino, Ida. AU - Suske, Guntram. AU - Ahmed, Naila. AU - Thornalley, Paul J.. AU - Sarthy, Vijay P.. AU - Hammes, Hans Peter. AU - Brownlee, Michael. PY - 2006/1/27. Y1 - 2006/1/27. N2 - Methylglyoxal is a highly reactive dicarbonyl degradation product formed from triose phosphates during glycolysis. Methylglyoxal forms stable adducts primarily with arginine residues of intracellular proteins. The biologic role of this covalent modification in regulating cell function is not known. Here, we report that in retinal Müller cells, increased glycolytic flux causes increased methylglyoxal modification of the corepressor mSin3A. Methylglyoxal modification of mSin3A results in increased recruitment of O-GlcNAc transferase to an mSin3A-Sp3 complex, with consequent increased ...
Angiopoietins and their receptor Tie-2 are, in concert with VEGF-A, key mediators in angiogenesis. This study evaluates the prognostic impact of all known human angiopoietins (Ang-1, Ang-2 and Ang-4) and their receptor Tie-2, as well as their relation to the prognostic expression of VEGF-A. 335 unselected stage I-IIIA NSCLC-patients were included and tissue samples of respective tumor cells and ...
This study demonstrates the distinct spatiotemporal expression profile of Angpt2 after MI and I/R, which, to date, has been obscure due to a lack of reliable antibodies (54). Using our recently developed anti-Angpt2 antibody (23), which is highly sensitive and specific to Angpt2, we demonstrate distinct expressions of Angpt2 on the ECs and proinflammatory macrophages after ischemia. This prompted us to further investigate the role of Angpt2 in postischemic cardiovascular remodeling. We recently reported that the FOXO1/Angpt2 axis suppresses the endothelial PI3K/Akt-signaling pathway, the main downstream pathway for Tie2 that plays a vital role in maintaining vascular integrity, inducing vascular destabilization in a positive feedback manner (25), and that it is central to the pathogenesis of sepsis (23), diabetic retinopathy (25), and tumor vessel destabilization (38). This study provides additional insights into the serious detrimental effects of the FOXO1/Angpt2 axis in exacerbating cardiac ...
Sphingosine-1-phosphate (S1P) is the endogenous ligand for the sphingosine-1-phophate receptors (S1P1-5) and triggers a number of cellular responses through their stimulation. S1P and its interaction with the S1P receptors play a significant role in a variety of biological processes including vascular stabilization, heart development, lymphocyte homing, and cancer angiogenesis. Agonism of S1P1, especially, has been shown to play an important role in lymphocyte trafficking from the thymus and secondary lymphoid organs, inducing immunosuppression, which has been established as a novel mechanism of treatment for immune diseases and vascular diseases. Sphingosine-1 -phosphate (SlP) has been demonstrated to induce many cellular effects, including those that result in platelet aggregation, cell proliferation, cell morphology, tumor cell invasion, endothelial cell and leukocyte chemotaxis, endothelial cell in vitro angiogenesis, and lymphocyte trafficking. SlP receptors are therefore good targets for a ...
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Rabbit polyclonal Angiopoietin 1 + Angiopoietin 2 antibody. Validated in WB, ICC/IF and tested in Rat, Human. Cited in 14 publication(s). Immunogen corresponding to synthetic peptide.
To investigate the theory, Bhandari and colleagues first tested a cohort of lung secretion samples taken from premature infants. After testing the samples, the researchers found that miR-34a was significantly increased only in the samples taken from babies who went on to develop BPD. The researchers also tested lung tissue collected postmortem from babies who had died at different ages from lung disease. Again, they found that this particular microRNA was expressed in the samples and also its downstream targets (the production of proteins) were suppressed in the ones with BPD. Next, the researchers found that levels of miR-34a levels were increased in the lungs of neonatal mice exposed to high levels of oxygen. Bhandari and his team then blocked the microRNA by repressing gene expression, as well as pharmacologically, by injecting the mouse models with the downstream target of the miR-34a, angiopoietin-1. Blocking miR-34a or injecting angiopoietin-1 in the mouse models improved their lung ...
Meet us at the ASBMR in Orlando at The Biomedica booth # 516 Our Posters: #SUN-103, #SUN-271, #MON-252 and #MON-331 Tje Bone Turnover Markers Working Group Room# W307B, Sept 20 at 7:30pm Diurnal Changes in Serum Levels of Bone-Related Circulating MicroRNAs. Oral presentation #1140, room# W314, Monday, Sept 23 from 2:15pm-2:30pm If you cannot make it to the ASBMR ...
The ANGPT-TIE2 pathway is important for angiogenesis, lymphangiogenesis and inflammation, and therefore has important roles in cancer. Given the context-dependent and opposing effects of the ANGPTs, how do we target this pathway? Angiopoietins (ANGPTs) are ligands of the endothelial cell receptor TIE2 and have crucial roles in the tumour angiogenic switch. Increased expression of ANGPT2 relative to ANGPT1 in tumours correlates with poor prognosis. The biological effects of the ANGPT-TIE system are context dependent, which brings into question what the best strategy is to target this pathway. This Review presents an encompassing picture of what we know about this important axis in tumour biology. The various options for
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Polyclonal antibody for Angiopoietin 2/ANGPT2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. Angiopoietin 2/ANGPT2 information: Molecular Weight: 56919 MW; Subcellular Localization: Secreted.
Yn aml mae gan enynnau lawer o gyfystyron. Mae hyn oherwydd eu bod yn aml yn cael eu darganfod gan nifer o bobl mewn cyd-destunau gwahanol heb wybod mair un genynnau oeddyn nhw. Hefyd mae gan wahanol gymunedau gwyddonol safonau gwahanol ar gyfer enwi genynnau. Dyma restr o gyfystyron ar gyfer y genyn ANGPT2. ...
Affiliation:名古屋市立大学,大学院医学研究科,講師, Research Field:Ophthalmology, Keywords:脈絡膜新生血管,炎症,加齢黄斑変性,ペリサイト,細胞生物学,リポフスチン,Angiopoietin-2,マウス,発現抑制,脂質, # of Research Projects:5, # of Research Products:33, Ongoing Project:加齢黄斑変性の病態におけるブルッフ膜への加齢性沈着脂質の役割の解明
Angiopoietin 2 antibody (angiopoietin 2) for ELISA, IHC-P, WB. Anti-Angiopoietin 2 pAb (GTX10601) is tested in Human samples. 100% Ab-Assurance.
Angiopoietin 1兔多克隆抗体(ab94684)可与小鼠, 人样本反应并经WB, IHC实验严格验证并得到1个独立的用户反馈。所有产品均提供质保服务,中国75%以上现货。
Published data on the role of the Ang/tie-2 system in cardiovascular diseases are limited. Most of our knowledge regarding the angiopoietins has come from oncology studies where angiogenesis is a prerequisite for tumor growth and metastasis. Indeed, Ang-2 has been shown to be a marker of a poor prognosis in breast cancer and non-small cell lung cancer (4,21). In the present paper, we describe a new assay for Ang-1 and, in addition, have demonstrated (for the first time in the literature) very abnormal levels of Ang-2 and tie-2, but normal Ang-1, in CHF. Vascular endothelial growth factor was marginally raised in the patients, suggesting that Ang-2 may be more relevant to the pathophysiology of this disease. In addition, there was a significant correlation between Ang-2 and tie-2.. That Ang-1 is not raised in CHF is consistent with currently held views that its secretion is not stimulated by hypoxia, as demonstrated in animal studies (13,14,22). Angiopoietin-1 has been shown to have antiapoptotic ...
Background: Microcirculatory dysfunction is associated with multiple organ failure and unfavorable patient outcome. We investigated whether therapeutically targeting the endothelial angiopoietin/Tie2 system preserves microvascular integrity during hemorrhagic shock. Methods: Rats were treated with the angiopoietin-1 mimetic vasculotide and subjected to hemorrhagic shock and fluid resuscitation. Microcirculatory perfusion and leakage were assessed with intravital microscopy (n = 7 per group) and Evans blue dye extravasation (n = 8 per group), respectively. The angiopoietin/Tie2 system was studied at protein and RNA level in plasma, kidneys, and lungs. Results: Hemorrhagic shock significantly reduced continuously perfused capillaries (7 ± 2 vs. 11 ± 2) and increased nonperfused vessels (9 ± 3 vs. 5 ± 2) during hemorrhagic shock, which could not be restored by fluid resuscitation. Hemorrhagic shock increased circulating angiopoietin-2 and soluble Tie2 significantly, which associated with ...
Angiopoietin-related protein 2 also known as angiopoietin-like protein 2 is a protein that in humans is encoded by the ANGPTL2 gene. Angiopoietin-like protein 2 maintains tissue homeostasis by promoting adaptive inflammation and subsequent tissue reconstruction, whereas an excess of ANGPTL2 activation induced by prolonged stress promotes the breakdown of tissue homeostasis due to chronic inflammation, promoting the development of metabolic diseases. ANGPTL2 has a role also in angiogenesis, in tissue repair, in obesity, in atherosclerotic diseases and finally in cancerogenesis. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. ANGPTL2 protein is a secreted glycoprotein with homology to the angiopoietins and may exert a function on endothelial cells through autocrine or paracrine action. GRCh38: ...
Notch and Angiopoietin-1 (Ang1)/Tie2 pathways are crucial for vascular maturation and stability. Here we identify the transcription factor ERG as a key regulator of endothelial Notch signalling. We show that ERG controls the balance between Notch ligands by driving Delta-like ligand 4 (Dll4) while repressing Jagged1 (Jag1) expression. In vivo, this regulation occurs selectively in the maturing plexus of the mouse developing retina, where Ang1/Tie2 signalling is active. We find that ERG mediates Ang1-dependent regulation of Notch ligands and is required for the stabilizing effects of Ang1 in vivo. We show that Ang1 induces ERG phosphorylation in a phosphoinositide 3-kinase (PI3K)/Akt-dependent manner, resulting in ERG enrichment at Dll4 promoter and multiple enhancers. Finally, we demonstrate that ERG directly interacts with Notch intracellular domain (NICD) and {beta}-catenin and is required for Ang1-dependent {beta}-catenin recruitment at the Dll4 locus. We propose that ERG coordinates Ang1, ...
Background. In sepsis and various other inflammatory conditions, elevated circulating levels of angiopoietin-2 (Ang2) are detected, but the precise functional role of Ang2 in these conditions is not well understood. Here, we investigated the contribution of Ang2 to the inflammatory response and renal function impairment in a mouse model of endotoxaemia.. Methods. Ang2-deficient mice and wild-type littermates were challenged with lipopolysaccharide [LPS; 1500 EU/g, intraperitoneal (i.p.)]. In additional experiments, wild-type C57Bl/6 mice were depleted of circulating neutrophils by antibody treatment (NIMPR14) prior to LPS challenge to study the role of neutrophils in regulating LPS-induced cytokine release. After 8 or 24 h of LPS challenge, the mice were sacrificed and organs were harvested. Quantitative reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay were performed for endothelial adhesion molecules (P-selectin, E-selectin, VCAM-1 and ICAM-1) and plasma ...
Hyperoxia-induced acute lung injury (HALI) is a key contributor to the pathogenesis of bronchopulmonary dysplasia (BPD) in neonates, for which no specific preventive or therapeutic agent is available. Here we show that lung micro-RNA (miR)-34a levels are significantly increased in lungs of neonatal mice exposed to hyperoxia. Deletion or inhibition of miR-34a improves the pulmonary phenotype and BPD-associated pulmonary arterial hypertension (PAH) in BPD mouse models, which, conversely, is worsened by miR-34a overexpression. Administration of angiopoietin-1, which is one of the downstream targets of miR34a, is able to ameliorate the BPD pulmonary and PAH phenotypes. Using three independent cohorts of human samples, we show that miR-34a expression is increased in type 2 alveolar epithelial cells in neonates with respiratory distress syndrome and BPD. Our data suggest that pharmacologic miR-34a inhibition may be a therapeutic option to prevent or ameliorate HALI/BPD in neonates.
Mice. Specific pathogen-free (SPF) C57BL/6J mice (catalog 000664), Tie2-GFP mice (catalog 003658), and UBC-Cre-ERT2 mice (catalog 007001) were purchased from the Jackson Laboratory. Angpt1fl/fl mice were a gift from Yoshikazu Nakaoka (Osaka University, Osaka, Japan), Angpt1-GFP mice were a gift from Sean J. Morrison (University of Texas Southwestern, Dallas, Texas, USA), Angpt2-lacZ mice were a gift from Nicholas Gale (Regeneron Pharmaceuticals), and Vegfr2fl/fl mice were a gift from Masanori Hirashima (Kobe University, Kobe, Japan). Prox1-GFP (30), Angpt1-GFP (33), Prox1fl/fl (39), Tie2fl/fl (35), Angpt1fl/fl (36, 37), Angpt2fl/fl (38), Vegfr2fl/fl (55), and Angpt2-lacZ (22) mice were transferred and bred in our SPF animal facility at KAIST. To deplete Prox1, Tie2, or Vegfr2 genes specifically in ECs, VE-cadherin-Cre-ERT2 mice (34) were mated with either Prox1fl/fl, Tie2fl/fl, or Vegfr2fl/fl mice to obtain EC-specific Prox1-, Tie2-, or Vegfr2-deleted mice, respectively, in a tamoxifen-dependent ...
Human platelet lysate (PL) is a cost-effective and human source of autologous multiple and potent pro-angiogenic factors, such as vascular endothelial growth factor A (VEGF A), fibroblast growth factor b (FGF b) and angiopoietin-1. Nanocoatings previously characterized were prepared by layer-by-layer assembling incorporating PL with marine-origin polysaccharides and were shown to activate human umbilical vein endothelial cells (HUVECs). Within 20 h of incubation, the more sulfated coatings induced the HUVECS to the form tube-like structures accompanied by an increased expression of angiogenic-associated genes, such as angiopoietin-1 and VEGF A. This may be a cost-effective approach to modify 2D/3D constructs to instruct angiogenic cells towards the formation of neo-vascularization, driven by multiple and synergistic stimulations from the PL combined with sulfated polysaccharides.. ...
ENCODES a protein that exhibits identical protein binding (ortholog); INVOLVED IN animal organ regeneration (ortholog); liver regeneration (ortholog); negative regulation of apoptotic process (ortholog); PARTICIPATES IN angiopoietin signaling pathway; ASSOCIATED WITH brain ischemia (ortholog); Diabetes Mellitus, Experimental (ortholog); diabetic retinopathy (ortholog); FOUND IN extracellular space (inferred); integral component of membrane (inferred); membrane raft (inferred)
Purified Recombinant Human ANGPT1/COMP protein, FLAG-tagged from Creative Biomart. Recombinant Human ANGPT1/COMP protein, FLAG-tagged can be used for research.
401366DNAHomo sapiens 1gaggtgcagc tggtggagtc tgggggaggc ttggtccagc ctggggggtc cctgagactc 60tcctgtgcag tctctggatt cacctttagt agctattgga tgagctgggt ccgccaggct 120ccagggaagg ggctggagtg ggtggccaac ataaagcaag atggaagtga gaaatactat 180gtggactctg tgaagggccg attcaccatc tccagagaca acgccaagaa ctcactgtat 240ctgcaaatga acagcctgag agccgaggac acggctgtgt attactgtgc gagagatcaa 300ggtatagcag tggctgggcc ctttgactac tggggccagg gaaccctggt caccgtctcc 360tcagcc 3662122PRTHomo Sapiens 2Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly1 5 10 15Ser Leu Arg Leu Ser Cys Ala Val Ser Gly Phe Thr Phe Ser Ser Tyr 20 25 30Trp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 35 40 45Ala Asn Ile Lys Gln Asp Gly Ser Glu Lys Tyr Tyr Val Asp Ser Val 50 55 60Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr65 70 75 80Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95Ala Arg Asp Gln Gly Ile Ala Val Ala Gly Pro Phe Asp Tyr Trp Gly 100 105 110Gln Gly Thr Leu Val Thr Val Ser ...
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Explore the angiopoietin-TIE2 signaling pathway and find antibodies to detect some of its target proteins, including CD202b (TIE2), AKT, ERK1, and ERK2.
ANGPT2 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ANGPT2. (KA1867) - Products - Abnova
Detect and quantitate human angiopoietin 1 (ANGPT1) in buffered solution and cell culture supernatants using a homogeneous LANCE Ultra TR-FRET assay.
Camenisch G et al. (2002) ANGPTL3 stimulates endothelial cell adhesion and migration via integrin alpha vbeta 3 and induces blood vessel formation in vivo.. [^] ...
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Utilization of cylindrical roller linear guide that up-level the parallelism between molds, and improve the cleanless circumstance around mold by lubrication-free on tie bar for its guider function has been replaced. ...
AGPT2 - Angpt2 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector shRNA available for purchase from OriGene - Your Gene Company.
Angiopoietin. *Agonists: Angiopoietin 1. *Angiopoietin 4. *Antagonists: Angiopoietin 2. *Angiopoietin 3. *Kinase inhibitors: ... Binding proteins: IGFBP (1, 2, 3, 4, 5, 6, 7). *Cleavage products/derivatives with unknown target: Glypromate (GPE, (1-3)IGF-1) ... 1][2][3] It belongs to the tyrosine kinase inhibitor family of medications.[4] It is taken by mouth.[4] ... LUX-Lung 1): a phase 2b/3 randomised trial". The Lancet. Oncology. 13 (5): 528-38. doi:10.1016/S1470-2045(12)70087-6. PMID ...
Angiopoietin. *Agonists: Angiopoietin 1. *Angiopoietin 4. *Antagonists: Angiopoietin 2. *Angiopoietin 3. *Kinase inhibitors: ... 6 (1): 79-85. doi:10.3758/CABN.6.1.79. PMID 16869232.. *^ a b c d e Baj G, Carlino D, Gardossi L, Tongiorgi E (October 2013). " ... doi:10.1016/S0896-6273(00)80540-1. PMID 9728912. S2CID 13983709.. *^ a b c d e Bartkowska K, Paquin A, Gauthier AS, Kaplan DR, ... 1 (3): 191-98. doi:10.1038/35044558. PMID 11257907. S2CID 9750498.. *^ a b Zhong L, Yan CH, Lu CQ, Xu J, Huang H, Shen XM ( ...
Angiopoietin. *Agonists: Angiopoietin 1. *Angiopoietin 4. *Antagonists: Angiopoietin 2. *Angiopoietin 3. *Kinase inhibitors: ... 1 (3): 263-268.. *^ a b "Eyetech Announces Approval of Macugen(R) in Brazil for the Treatment of Neovascular (Wet) Age-Related ... Binding proteins: IGFBP (1, 2, 3, 4, 5, 6, 7). *Cleavage products/derivatives with unknown target: Glypromate (GPE, (1-3)IGF-1) ... 1 mg, or 3 mg of pegaptanib administered intravitreal injections every 6 weeks. Of the 1200 patients enrolled in the study ...
Angiopoietin. *Agonists: Angiopoietin 1. *Angiopoietin 4. *Antagonists: Angiopoietin 2. *Angiopoietin 3. *Kinase inhibitors: ... Binding proteins: IGFBP (1, 2, 3, 4, 5, 6, 7). *Cleavage products/derivatives with unknown target: Glypromate (GPE, (1-3)IGF-1) ... PDGF[1][2] is a potent mitogen for cells of mesenchymal origin, including fibroblasts, smooth muscle cells and glial cells. In ... 978-1-4160-3121-5. .. *^ Fredriksson, Linda; Li, Hong; Eriksson, Ulf (August 2004). "The PDGF family: four gene products form ...
Angiopoietin. *Agonists: Angiopoietin 1. *Angiopoietin 4. *Antagonists: Angiopoietin 2. *Angiopoietin 3. *Kinase inhibitors: ... 6 (1): 79-85. doi:10.3758/CABN.6.1.79. PMID 16869232.. *^ a b c d e Baj G, Carlino D, Gardossi L, Tongiorgi E (October 2013). " ... doi:10.1016/S0896-6273(00)80540-1. PMID 9728912.. *^ a b c d e Bartkowska K, Paquin A, Gauthier AS, Kaplan DR, Miller FD ( ... 41 (1): 22-28. doi:10.1002/eat.20474. PMID 17922530.. *^ a b Vargas-Perez H, Ting-A Kee R, Walton CH, Hansen DM, Razavi R, ...
Angiopoietin. *Agonists: Angiopoietin 1. *Angiopoietin 4. *Antagonists: Angiopoietin 2. *Angiopoietin 3. *Kinase inhibitors: ... 1 February 2008. Retrieved 14 November 2019.. *^ a b c d e f g h "Nilotinib Monograph for Professionals". Drugs.com. Retrieved ... Binding proteins: IGFBP (1, 2, 3, 4, 5, 6, 7). *Cleavage products/derivatives with unknown target: Glypromate (GPE, (1-3)IGF-1) ... 55 (10): 1-6. doi:10.3109/10428194.2014.887714. PMID 24467220.. *^ "FDA Approves Tasigna for Treatment of Philadelphia ...
Angiopoietin. *Agonists: Angiopoietin 1. *Angiopoietin 4. *Antagonists: Angiopoietin 2. *Angiopoietin 3. *Kinase inhibitors: ... This article on a gene on human chromosome 1 is a stub. You can help Wikipedia by expanding it.. *v ... Binding proteins: IGFBP (1, 2, 3, 4, 5, 6, 7). *Cleavage products/derivatives with unknown target: Glypromate (GPE, (1-3)IGF-1) ... 95 (1): 221-30. PMID 10607706.. *. Munthe E, Aasheim HC (2002). "Characterization of the human ephrin-A4 promoter". Biochem. J ...
Angiopoietin. *Agonists: Angiopoietin 1. *Angiopoietin 4. *Antagonists: Angiopoietin 2. *Angiopoietin 3. *Kinase inhibitors: ... 15 (1): 11-5. doi:10.1590/s1555-79602013000100004. PMID 23396236.. *^ Yang S, Geng Z, Ma K, Sun X, Fu X (June 2016). "Efficacy ... 6 (1): e1231276. doi:10.1080/21592535.2016.1231276. PMC 5098722. PMID 27740881.. *^ Tenchurin, T.H.; Lyundup, A.V.; Demchenko, ... Binding proteins: IGFBP (1, 2, 3, 4, 5, 6, 7). *Cleavage products/derivatives with unknown target: Glypromate (GPE, (1-3)IGF-1) ...
Angiopoietin. *Agonists: Angiopoietin 1. *Angiopoietin 4. *Antagonists: Angiopoietin 2. *Angiopoietin 3. *Kinase inhibitors: ... Binding proteins: IGFBP (1, 2, 3, 4, 5, 6, 7). *Cleavage products/derivatives with unknown target: Glypromate (GPE, (1-3)IGF-1) ... 156 (1): 16-23. doi:10.1210/en.2014-1596. PMID 25330101.. *^ Gravanis A, Calogeropoulou T, Panoutsakopoulou V, Thermos K, ... 3 (1): 19-24. doi:10.2174/156720506775697061. PMID 16472198.. *^ Reichardt LF (September 2006). "Neurotrophin-regulated ...
Angiopoietin. *Agonists: Angiopoietin 1. *Angiopoietin 4. *Antagonists: Angiopoietin 2. *Angiopoietin 3. *Kinase inhibitors: ... Binding proteins: IGFBP (1, 2, 3, 4, 5, 6, 7). *Cleavage products/derivatives with unknown target: Glypromate (GPE, (1-3)IGF-1) ... 1 Reverse signaling in growth cone survival *1.1 Formation of the retinotopic map ... 1995). "Cloning of AL-1, a ligand for an Eph-related tyrosine kinase receptor involved in axon bundle formation". Neuron. 14 (5 ...
Angiopoietin. *Agonists: Angiopoietin 1. *Angiopoietin 4. *Antagonists: Angiopoietin 2. *Angiopoietin 3. *Kinase inhibitors: ... Binding proteins: IGFBP (1, 2, 3, 4, 5, 6, 7). *Cleavage products/derivatives with unknown target: Glypromate (GPE, (1-3)IGF-1) ... 2009 October 1; 119(10). Tang, Dual MET EGFR combinatorial inhibition against T790M-EGFR-mediated erlotinib-resistant lung ... Blum G, Gazit A, Levitzki A (2000). "Substrate competitive inhibitors of IGF-1 receptor kinase". Biochemistry. 39 (51): 15705- ...
Angiopoietin. *Agonists: Angiopoietin 1. *Angiopoietin 4. *Antagonists: Angiopoietin 2. *Angiopoietin 3. *Kinase inhibitors: ... Binding proteins: IGFBP (1, 2, 3, 4, 5, 6, 7). *Cleavage products/derivatives with unknown target: Glypromate (GPE, (1-3)IGF-1) ... 38 (1): 11-9. doi:10.1080/07853890500442758. PMID 16448984.. *. Ciampi R, Nikiforov YE (2007). "RET/PTC rearrangements and BRAF ... doi:10.1385/EP:13:1:03. PMID 12114746.. *. Santoro M, Melillo RM, Carlomagno F, et al. (2004). "Minireview: RET: normal and ...
Angiopoietin. *Agonists: Angiopoietin 1. *Angiopoietin 4. *Antagonists: Angiopoietin 2. *Angiopoietin 3. *Kinase inhibitors: ... 1 November 2006. Retrieved 10 June 2020.. *^ a b c "FDA Highlights and Prescribing Information for Gleevec(imatinib mesylate)" ... Retrieved 1 April 2017.. *^ a b "Gleevec Gains Simultaneous FDA Approval for Five Rare, Life-Threatening Disorders". Cancer ... Archived from the original on 1 February 2014.. *^ a b c "Gleevec (imatinib) dosing, indications, interactions, adverse effects ...
Angiopoietin. *Agonists: Angiopoietin 1. *Angiopoietin 4. *Antagonists: Angiopoietin 2. *Angiopoietin 3. *Kinase inhibitors: ... Binding proteins: IGFBP (1, 2, 3, 4, 5, 6, 7). *Cleavage products/derivatives with unknown target: Glypromate (GPE, (1-3)IGF-1) ... Fibroblast growth factor 20 is a protein which in humans is encoded by the FGF20 gene.[1] ...
Angiopoietin. *Agonists: Angiopoietin 1. *Angiopoietin 4. *Antagonists: Angiopoietin 2. *Angiopoietin 3. *Kinase inhibitors: ... Binding proteins: IGFBP (1, 2, 3, 4, 5, 6, 7). *Cleavage products/derivatives with unknown target: Glypromate (GPE, (1-3)IGF-1) ... 108 (1): 1-10. PMID 8061955.. *. Gratacòs M, EscaramÃs G, Bustamante M, et al. (2010). "Role of the neurotrophin network in ...
Angiopoietin. *Agonists: Angiopoietin 1. *Angiopoietin 4. *Antagonists: Angiopoietin 2. *Angiopoietin 3. *Kinase inhibitors: ... Binding proteins: IGFBP (1, 2, 3, 4, 5, 6, 7). *Cleavage products/derivatives with unknown target: Glypromate (GPE, (1-3)IGF-1) ...
Angiopoietin (Ang). *Autocrine motility factor. *Bone morphogenetic proteins (BMPs). *Ciliary neurotrophic factor family * ... Binding proteins: IGFBP (1, 2, 3, 4, 5, 6, 7). *Cleavage products/derivatives with unknown target: Glypromate (GPE, (1-3)IGF-1) ... A growth factor is a naturally occurring substance capable of stimulating cellular growth,[1] proliferation, healing, and ... The alpha granules in blood platelets contain growth factors PDGF, IGF-1, EGF, and TGF-β which begin healing of wounds by ...
Angiopoietin. *Agonists: Angiopoietin 1. *Angiopoietin 4. *Antagonists: Angiopoietin 2. *Angiopoietin 3. *Kinase inhibitors: ... Binding proteins: IGFBP (1, 2, 3, 4, 5, 6, 7). *Cleavage products/derivatives with unknown target: Glypromate (GPE, (1-3)IGF-1) ... KGF is a small signaling molecule that binds to fibroblast growth factor receptor 2b (FGFR2b).[1] For signalling to occur, a ...
Angiopoietin. *Agonists: Angiopoietin 1. *Angiopoietin 4. *Antagonists: Angiopoietin 2. *Angiopoietin 3. *Kinase inhibitors: ... Binding proteins: IGFBP (1, 2, 3, 4, 5, 6, 7). *Cleavage products/derivatives with unknown target: Glypromate (GPE, (1-3)IGF-1) ... 156 (1): 16-23. doi:10.1210/en.2014-1596. PMID 25330101.. *^ Gravanis A, Calogeropoulou T, Panoutsakopoulou V, Thermos K, ... doi:10.1007/s00018-006-6010-1. PMID 16699811.. *^ Harrington AW, Ginty DD (March 2013). "Long-distance retrograde neurotrophic ...
Angiopoietin. *Agonists: Angiopoietin 1. *Angiopoietin 4. *Antagonists: Angiopoietin 2. *Angiopoietin 3. *Kinase inhibitors: ... 27 (1): 168-70. doi:10.1017/S1041610214001616. PMC 4501575. PMID 25101659.. *^ a b Nykjaer A, Lee R, Teng KK, Jansen P, Madsen ... 978-0-444-51472-1. . PMID 14699960.. *^ a b c Lee R, Kermani P, Teng KK, Hempstead BL (Nov 2001). "Regulation of cell survival ... 8 (1): 43-48. PMC 3749761. PMID 24023598.. *^ a b Wiesmann C, Ultsch MH, Bass SH, de Vos AM (September 1999). "Crystal ...
Angiopoietin. *Agonists: Angiopoietin 1. *Angiopoietin 4. *Antagonists: Angiopoietin 2. *Angiopoietin 3. *Kinase inhibitors: ... 9 (1): 83-90. doi:10.3892/mmr.2013.1781. PMID 24213221.. *^ Gauthier A (Feb 2013). "Role of sorafenib in the treatment of ... Binding proteins: IGFBP (1, 2, 3, 4, 5, 6, 7). *Cleavage products/derivatives with unknown target: Glypromate (GPE, (1-3)IGF-1) ... 1 (1): 1-3. doi:10.4021/jmc112e. Retrieved 11 February 2014.. *^ FDA Approval letter for use of sorafenib in advanced renal ...
Angiopoietin. *Agonists: Angiopoietin 1. *Angiopoietin 4. *Antagonists: Angiopoietin 2. *Angiopoietin 3. *Kinase inhibitors: ... 22 (1): 175-84. doi:10.1200/JCO.2004.05.114. PMID 14701780.. *^ a b Rao, S; Starling, N; Cunningham, D; Benson, M; Wotherspoon ... Binding proteins: IGFBP (1, 2, 3, 4, 5, 6, 7). *Cleavage products/derivatives with unknown target: Glypromate (GPE, (1-3)IGF-1) ... It binds to the epidermal growth factor receptor (EGFR) with high affinity.[1] The mouse monoclonal antibody (mAb425) from ...
Angiopoietin. *Agonists: Angiopoietin 1. *Angiopoietin 4. *Antagonists: Angiopoietin 2. *Angiopoietin 3. *Kinase inhibitors: ... Binding proteins: IGFBP (1, 2, 3, 4, 5, 6, 7). *Cleavage products/derivatives with unknown target: Glypromate (GPE, (1-3)IGF-1) ... "Flk-1 as a target for tumor growth inhibition". Cancer Res. 56 (15): 3540-5. PMID 8758924.. ... 1] which was the first description of compounds inhibiting the catalytic activity of the epidermal growth factor receptor (EGFR ...
Angiopoietin. *Agonists: Angiopoietin 1. *Angiopoietin 4. *Antagonists: Angiopoietin 2. *Angiopoietin 3. *Kinase inhibitors: ... 1 (6): 588-90. doi:10.1097/01243894-200607000-00016. PMID 17409923.. *^ He J, Zhang A, Fang M, Fang R, Ge J, Jiang Y, Zhang H, ... Binding proteins: IGFBP (1, 2, 3, 4, 5, 6, 7). *Cleavage products/derivatives with unknown target: Glypromate (GPE, (1-3)IGF-1) ... IGF-2 exerts its effects by binding to the IGF-1 receptor and to the short isoform of the insulin receptor (IR-A or exon 11-).[ ...
Angiopoietin. *Agonists: Angiopoietin 1. *Angiopoietin 4. *Antagonists: Angiopoietin 2. *Angiopoietin 3. *Kinase inhibitors: ... Binding proteins: IGFBP (1, 2, 3, 4, 5, 6, 7). *Cleavage products/derivatives with unknown target: Glypromate (GPE, (1-3)IGF-1) ... Vandetanib is used to treat medullary thyroid cancer in adults who are ineligible for surgery.[1][5][6] ... Common (present in between 1% and 10% of people) adverse effects include pneumonia, sepsis, influenza, cystitis, sinusitis, ...
Angiopoietin 2. antagonist of angiopoietin 1. TSP-1 and TSP-2. inhibit cell migration, cell proliferation, cell adhesion and ... Meth-1 and Meth-2. IFN-α, -β and -γ, CXCL10, IL-4, -12 and -18. inhibit cell migration of endothelial cells, downregulate bFGF ... 25 (1): 1-10. doi:10.1002/ptr.3224. PMID 20564543.. *^ Izuta, Hiroshi; Chikaraishi, Yuichi; Shimazawa, Masamitsu; Mishima, ... doi:10.1016/s1357-2725(01)00023-1. PMID 11312106.. *^ Rini, BI. (Feb 2007). "Vascular endothelial growth factor-targeted ...
Angiopoietin-1 receptor also known as CD202B (cluster of differentiation 202B) is a protein that in humans is encoded by the ... Master Z, Jones N, Tran J, Jones J, Kerbel RS, Dumont DJ (November 2001). "Dok-R plays a pivotal role in angiopoietin-1- ... The ligand for the receptor is angiopoietin-1. TEK has also been suggested as a marker for nucleus pulposus progenitor cells, ... Also known as TIE2, it is an angiopoietin receptor. The TEK receptor tyrosine kinase is expressed almost exclusively in ...
Li JJ, Huang YQ, Basch R, Karpatkin S (February 2001). "Thrombin induces the release of angiopoietin-1 from platelets". ... Angiopoietin-1) and cytokines including the EMT inducer TGF-β. The release of TGF-β by platelets in blood vessels near primary ... 201 (1): 27-36. doi:10.1677/joe-08-0497. PMID 19171567. Jolly MK, Boareto M, Huang B, Jia D, Lu M, Ben-Jacob E, et al. (1 ... 270 (1-2): 87-93. doi:10.1016/j.mce.2007.02.005. PMC 1987709. PMID 17363142. Russ HA, Bar Y, Ravassard P, et al. (2008). "In ...
Angiopoietin 2 can act as an antagonist to Tie-2, destabilizing the endothelial cells, which results in less endothelial cell ... Angiopoietin 1 and Tie-2 signaling is essential for maturation and stabilization of endothelial cells. Platelet-derived growth ... Similar to the inhibition of the PDGF pathway, angiopoietin 2 reduces levels of pericytes, leading to diabetic retinopathy. ... "Angiopoietin-2, a natural antagonist for Tie2 that disrupts in vivo angiogenesis". Science. 277 (5322): 55-60. doi:10.1126/ ...
Mammoto T, Parikh SM, Mammoto A, Gallagher D, Chan B, Mostoslavsky G, Ingber DE, Sukhatme VP (Aug 2007). "Angiopoietin-1 ... 257 (1): 23-31. doi:10.1016/S0378-1119(00)00387-5. PMID 11054565. Nagase T, Kikuno R, Hattori A, Kondo Y, Okumura K, Ohara O ( ... 23 (1): 94-101. doi:10.1038/nbt1046. PMID 15592455. S2CID 7200157. Barberis D, Casazza A, Sordella R, Corso S, Artigiani S, ... Glucocorticoid receptor DNA-binding factor 1 is a protein that in humans is encoded by the GRLF1 gene. The human glucocorticoid ...
In the combined absence of angiopoietin 1 and angiopoietin 2, Schlemm's canal and episcleral lymphatic vasculature completely ... 1][2][3][4] It is named after Friedrich Schlemm (1795-1858), a German anatomist. ...
... dan angiopoietin-1, hingga menghasilkan neuroblas dengan jarak tempuh migrasi yang lebih panjang dan rentang waktu sitokinesis ... Sel TH1 CD4+ dengan sekresi sitokina pro-radang termasuk IL-2, IL-12, IFN-γ dan TNF-α dapat memperburuk efek yang ditimbulkan ... Niken Prathivi (July 1, 2014). "Detecting and dealing with strokes".. *^ Donnan GA, Fisher M, Macleod M, Davis SM (May 2008). " ... Aktivasi faktor transkripsi ini akan menginduksi produksi sitokina radang seperti IL-1, IL-6, TNF-α, kemokina seperti IL-8, MCP ...
Angiopoietin-2 works with VEGF to facilitate cell proliferation and migration of endothelial cells ... 23 (1): 7-17. doi:10.1097/00004872-200501000-00004. PMID 15643116.. *^ Roberts OL, Holmes K, Müller J, Cross DA, Cross MJ (Dec ... Endothelium refers to cells that line the interior surface of blood vessels and lymphatic vessels,[1] forming an interface ...
Methylglyoxal modification of mSin3A links glycolysis to angiopoietin-2 transcription. Cell 2006; 124, 275-286 Abstract ... Inpharma 2003;1(1386)6 *↑ Schupp N, Schmid U, Heidland A, Stopper H. New approaches for the treatment of genomic damage in end- ... 2006;530(1-2):48-53. Abstract *↑ Cameron NE, Gibson TM, Nahgle MR, Cotter MA. Inhibitors of advanced glycation end product ... 2006;1(6):1293-9 Fuill Free Text *↑ Wu S, Ren J. Benfotiamine alleviates diabetes-induced cerebral oxidative damage independent ...
December 1996). "Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning". Cell. 87 ( ... Yancopoulos has cloned novel families of growth factors, including ephrins/Ephs and angiopoietins, and elucidated the basis of ... 9 (1): 47-52. doi:10.1038/nm811. PMID 12483208.. Appearances[edit]. Yancopoulos has been confirmed to speak at the annual drug ... Retrieved May 1, 2011.. *^ "Dr. George D. Yancopoulos, Founding Scientist, Regeneron Laboratories, to Be Honored by Burke ...
... angiopoietin-2, osteoprotegerin, P- selektin kofaktor CD63/lamp3,[9] i α-1,3-fukoziltransferaza VI. ... ISBN 9958-9344-1-8. *^ Weibel ER, Palade GE; Palade (October 1964). "New cytoplasmic components in arterial endothelia". J. ... Bonfanti, R.; Furie, B. C.; Furie, B.; Wagner, D. D. (1 April 1989). "PADGEM (GMP140) is a component of Weibel-Palade bodies of ... VWD, u nekim ljudskim populacijama, ima procijenjenu prevalenciju do 1%, a najčešće je obilježena produženim i varijabilnim ...
"GPIHBP1 stabilizes lipoprotein lipase and prevents its inhibition by angiopoietin-like 3 and angiopoietin-like 4". The Journal ... angiopoietin-like protein 4, and glycosylphosphatidylinositol-anchored high-density lipoprotein binding protein 1 in subjects ... GPIHBP1‏ (Glycosylphosphatidylinositol anchored high density lipoprotein binding protein 1) هوَ بروتين يُشَفر بواسطة جين ... "Entrez Gene: Glycosylphosphatidylinositol anchored high density lipoprotein binding protein 1". مؤرشف من الأصل في 23 أكتوبر ...
Mediators that promote angiogenesis are angiopoietin and vascular endothelial growth factor (VEGF). Prostaglandin E2 promotes ... Tanaka, Y; Ohno, K (1 June 2013). "Chronic subdural hematoma - an up-to-date concept" (PDF). Journal of Medical and Dental ... Signs and symptoms of acute hematomas may appear in minutes, if not immediately,[1] but can also be delayed as much as two ... 49 (1): 3-10. PMID 11303234. Retrieved 26 November 2017. . The possibility of direct effect of some vasoactive substances ...
... increased angiopoietin-2, and an elevated ratio of angiopoietin-2/angiopoietin-1 in the placenta. This suggests that ... Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar ... may be associated with a dysregulation in angiopoietins. Increased levels of angiopoietin-1 appear to be associated with a ... Angiopoietin 1 has been shown to interact with TEK tyrosine kinase. Recently, studies in malaria-endemic areas suggest that ...
2005). "Biological characterization of angiopoietin-3 and angiopoietin-4". FASEB J. 18 (11): 1200-8. doi:10.1096/fj.03-1466com ... 1999). "Molecular cloning, expression, and characterization of angiopoietin-related protein. angiopoietin-related protein ... angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. The protein encoded by this gene is another ... "Molecular cloning and characterization of a novel angiopoietin family protein, angiopoietin-3". FEBS Lett. 443 (3): 353-6. doi: ...
Buy our Recombinant human Angiopoietin 1 protein. Ab69492 is an active full length protein produced in HeLa cells and has been ... Recombinant human Angiopoietin 1 protein. See all Angiopoietin 1 proteins and peptides. ...
Angiopoietin-1 for Myocardial Angiogenesis , IntechOpen, Published on: 2011-10-21. Authors: Vien Khach Lai, Muhammad Zeeshan ... Angiopoietin-1 for Myocardial Angiogenesis. By Vien Khach Lai, Muhammad Zeeshan Afzal, Muhammad Ashraf and Khawaja Husnain ...
Angiopoietin-like protein 1 suppresses SLUG to inhibit cancer cell motility. Tsang-Chih Kuo,1 Ching-Ting Tan,2 Yi-Wen Chang,3,4 ... a family of proteins structurally similar to the angiopoietins was identified as angiopoietin-like proteins (ANGPTLs), which ... Angiopoietin-like 4 interacts with integrins beta1 and beta5 to modulate keratinocyte migration. Am J Pathol. 2010;177(6):2791- ... Angiopoietin-like proteins stimulate ex vivo expansion of hematopoietic stem cells. Nat Med. 2006;12(2):240-245.. View this ...
Angiopoietin-1 Uncouples the VEGF Receptor from Src Message Subject. (Your Name) has forwarded a page to you from Science ... J. S. Gutkind, Angiopoietin-1 prevents VEGF-induced endothelial permeability by sequestering Src through mDia. Dev. Cell 14, 25 ... Angiopoietin-1 (Ang1) and vascular endothelial growth factor (VEGF) play complementary roles in vascular development during ... extracellular signal-regulated kinases 1 and 2, and focal adhesion kinase) but did prevent disruption of endothelial barrier ...
Angiopoietin 2 antibody. Validated in WB, ICC/IF and tested in Rat, Human. Cited in 14 publication(s). Immunogen corresponding ... as this antibody recognizes Angiopoietin 2 in western blot without cross reactivity with Angiopoietin 1. Please let me know ... Anti-Angiopoietin 1 + Angiopoietin 2 antibody (ab65835) at 2 µg/ml + rat brain tissue lysate. Predicted band size: 57 kDa. ... As for ab65835 we have found that cross reacting with angiopoietin 1 and therefore we would be happy to either replace this ...
We previously identified loss-of-function mutations in the angiopoietin (ANGPT) receptor TEK in families with PCG and showed ... Angiopoietin-1 is required for Schlemms canal development in mice and humans. ... Angiopoietin-1 is required for Schlemms canal development in mice and humans. ... Published December 1, 2017; First published November 6, 2017 Citation Information: J Clin Invest. 2017;127(12):4421-4436. https ...
Expression of angiopoietin 1 and 2 in ectopic endometrium on the chicken chorioallantoic membrane. Drenkhahn, M., Gescher, D.M ... Expression of angiopoietin 1 and 2 in ectopic endometrium on the chicken chorioallantoic membrane [1]. ... OBJECTIVE: To investigate the role of angiopoietin 1 and 2 (ANGPT1/ANGPT2) in angiogenesis of the ectopic endometrium as a ... Gene expression of members of the VEGF and Angiopoietin families of angiogenic growth factors has been linked to haemorrhaging ...
Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning.. Davis S1, Aldrich TH, Jones ... The structure of Angiopoietin-1 differs from that of known angiogenic factors or other ligands for receptor tyrosine kinases. ... Although Angiopoietin-1 binds and induces the tyrosine phosphorylation of TIE2, it does not directly promote the growth of ... We report the identification of a secreted ligand for TIE2, termed Angiopoietin-1, using a novel expression cloning technique ...
Browse our Angiopoietin-like Protein 1/ANGPTL1 Protein catalog backed by our Guarantee+. ... Angiopoietin-like Protein 1/ANGPTL1 Proteins available through Novus Biologicals. ... angiopoietin Y1 protein, angiopoietin-3 protein, angiopoietin-like 1 protein, Angiopoietin-like protein 1 protein, angiopoietin ... Our Angiopoietin-like Protein 1/ANGPTL1 Peptides and Angiopoietin-like Protein 1/ANGPTL1 Proteins can be used in a variety of ...
... angiopoietin 3 , angiopoietin Y1 , angiopoietin-3 , dJ595C2.2 (angiopoietin Y1) , angiopoietin-like protein ... angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. The protein encoded by this gene is another ... Angiopoietin-Like 1 (ANGPTL1) Antigen-Profil Beschreibung des Gens Angiopoietins are members of the vascular endothelial growth ... Top Angiopoietin-Like 1 ELISA Kits auf antikoerper-online.de. Showing 10 out of 38 products:. Katalog Nr.. Reaktivität. ...
Requisite role of angiopoietin-1, a ligand for the TIE2 receptor, during embryonic angiogenesis.. Suri C1, Jones PF, Patan S, ... Angiopoietin-1 seems to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix ... We have recently cloned a ligand for TIE2, termed Angiopoietin-1. Here we show that mice engineered to lack Angiopoietin-1 ... demonstrating that Angiopoietin-1 is a primary physiologic ligand for TIE2 and that it has critical in vivo angiogenic actions ...
High sensitivity ELISA kit for detection of Angiopoietin-1. Backed by our 100% Guarantee. ... Gomes L, Alves-Junior E, Rodrigues-Jesus C, Nery A, Gasquez-Martin T, Fontes C Angiopoietin-2 and angiopoietin-2/angiopoietin-1 ... Angiopoietin-2, Angiopoietin-1 and subclinical cardiovascular disease in Chronic Kidney Disease Sci Rep, 2016;6(0):39400. 2016 ... Human Angiopoietin-1 Quantikine ELISA Kit Summary. Background. The Quantikine Human Angiopoietin-1 Immunoassay is a 4.5 hour ...
... Nat Med. 2000 Apr;6(4):460-3. doi: 10.1038/74725. ... To be useful clinically, angiopoietin-1 would have to inhibit leakage when acutely administered to adult vessels, and this ... Here we show that acute administration of angiopoietin-1 does indeed protect adult vasculature from leaking, countering the ... 12-14), and have shown that transgenic angiopoietin-1 acts reciprocally as an anti-permeability factor when provided ...
Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning. Cell 1996;87:1161-1169pmid: ... Angiopoietin-1 promotes neurite outgrowth from dorsal root ganglion cells positive for Tie-2 receptor. Cell Tissue Res 2005;320 ... Designed angiopoietin-1 variant, COMP-angiopoietin-1, rescues erectile function through healthy cavernous angiogenesis in a ... Effect of intracavernous administration of angiopoietin-4 on erectile function in the streptozotocin-induced diabetic mouse. J ...
Holopainen T, Huang H, Chen C, Kim KE, Zhang L, Zhou F, Han W, Li C, Yu J, Wu J, Koh GY, Alitalo K, He Y. Angiopoietin-1 ... Correction: Article on Role of Angiopoietin-1 in Tumor Metastasis Message Subject (Your Name) has forwarded a page to you from ... In the article on the role of angiopoietin-1 in tumor metastasis in the June 1, 2009 issue of Cancer Research ( 1), the authors ... Tanja Holopainen,2 Huilian Huang,1 Caiping Chen,1 Kyung Eun Kim,3 Luqing Zhang,1 Fei Zhou,1 Wencan Han,1 Chaojun Li,1 Jun Yu,4 ...
p,The LEGENDplex™ Human Angiogenesis Panel 1 Mix and Match Capture Beads offer flexible customization within the panel (12-plex ... 740701 LEGENDplex™ Human Angiopoietin-1 Capture Bead A5, 13X. 740702 LEGENDplex™ Human Angiopoietin-2 Capture Bead A6, 13X. ... Human Angiopoietin-1 Capture Bead A5, 13X (Cat. # 740701). • Human Angiogenesis Panel 1 Detection Antibodies (Cat. # 740700). ... Human Angiopoietin-1 Capture Bead A5, 13X (Cat. # 740701). • Human Angiogenesis Panel 1 Detection Antibodies (Cat. # 740700). ...
Angiopoietin-2 dose-dependently blocks directed migration toward Angiopoietin-1. Carlson et al (2001) have shown that Ang-1 ... Angiopoietins can potentiate the effects of other angiogenic cytokines. An investigation of the impact of angiopoietins on ... Angiopoietin 1, KIAA0003, ANG-1, AGP1, AGPT.. Introduction. ANGPT1 is an angiogenic factor which intervenes in blood vessel ... Angiopoietin-1 is a ligand for the receptor-like tyrosine kinase designated TIE-2 (Davis et al, 1996). Binding of Angiopoietin- ...
1. Dongjun Luo, Zhongxia Wang, Junyi Wu, Chunping Jiang, Junhua Wu, The Role of Hypoxia Inducible Factor-1 in Hepatocellular ... Hypoxia-inducible factor 1 alpha-activated angiopoietin-like protein 4 contributes to tumor metastasis via vascular cell ... Corrections: Hypoxia-inducible factor 1 alpha-activated angiopoietin-like protein 4 contributes to tumor metastasis via ... adhesion molecule-1/integrin β1 signaling in human hepatocellular carcinoma Volume 54, Issue 3, 910-919, Article first ...
Leakage-Resistant Blood Vessels in Mice Transgenically Overexpressing Angiopoietin-1. By G. Thurston, C. Suri, K. Smith, J. ... Leakage-Resistant Blood Vessels in Mice Transgenically Overexpressing Angiopoietin-1. By G. Thurston, C. Suri, K. Smith, J. ... Angiopoietin-1 (Ang1) and vascular endothelial growth factor (VEGF) are endothelial cell-specific growth factors. Direct ... Angiopoietin-1 (Ang1) and vascular endothelial growth factor/vascular permeability factor (VEGF) play separate but essential ...
Kwak HJ, So JN, Lee SJ, Kim I, Koh GY: Angiopoietin-1 is an apoptosis survival factor for endothelial cells. FEBS Lett448 :249 ... Daly C, Wong V, Burova E, Wei Y, Zabski S, Griffiths J, Lai KM, Lin HC, Ioffe E, Yancopoulos GD, Rudge JS: Angiopoietin-1 ... Cho CH, Sung HK, Kim KT, Cheon HG, Oh GT, Hong HJ, Yoo OJ, Koh GY: COMP-angiopoietin-1 promotes wound healing through enhanced ... Brindle NP, Saharinen P, Alitalo K: Signaling and functions of angiopoietin-1 in vascular protection. Circ Res98 :1014 -1023, ...
OMIM: ANGIOPOIETIN-LIKE 1; ANGPTL1*Gene Ontology: Angptl1 *Mouse Phenome DB: Angptl1 *UCSC: Chr.1:156,839,023-156,861,078(+)* ... angiopoietin-like 1. Synonyms: 2810039D03Rik, ANG-3, ANG3, ANGPT3, ANGY, ARP1. Gene nomenclature, locus information, and GO, ... Question? Comments? For Mice, Cells, and germplasm please contact us at [email protected], US 1-888-KOMP-MICE or International ... Vega: OTTMUSG22614 (Angptl1, angiopoietin-like 1)*CCDS: 15397, 15397.1* ...
We also tested the association of concentration of angiopoietin-2 relative to ang ... To test the hypothesis that the concentration of angiopoietin-2 relative to angiopoietin-1 may be a useful biological marker of ... Baseline concentration of angiopoietin-2 relative to angiopoietin-1 was significantly higher in patients who died (median, 58 [ ... OBJECTIVE: To test the hypothesis that the concentration of angiopoietin-2 relative to angiopoietin-1 may be a useful ...
Angiopoietin-1 Promotes Skeletal and Cardiac Myocyte Survival Through Integrins. Susan M. Dallabrida, Nesreen Ismail, Julianne ... Angiopoietin-1 Promotes Skeletal and Cardiac Myocyte Survival Through Integrins. Susan M. Dallabrida, Nesreen Ismail, Julianne ... Angiopoietin-1 Promotes Skeletal and Cardiac Myocyte Survival Through Integrins. Susan M. Dallabrida, Nesreen Ismail, Julianne ...
Dual angiopoietin-2 and VEGFA inhibition elicits antitumor immunity that is enhanced by PD-1 checkpoint blockade ... Dual angiopoietin-2 and VEGFA inhibition elicits antitumor immunity that is enhanced by PD-1 checkpoint blockade ... Dual angiopoietin-2 and VEGFA inhibition elicits antitumor immunity that is enhanced by PD-1 checkpoint blockade ... Dual angiopoietin-2 and VEGFA inhibition elicits antitumor immunity that is enhanced by PD-1 checkpoint blockade ...
The Effects of Angiopoietin-1 and -2 on Tumor Growth and Angiogenesis in Human Colon Cancer. Syed A. Ahmad, Wenbiao Liu, Young ... In situ expression of angiopoietins in astrocytomas identifies angiopoietin-2 as an early marker of tumor angiogenesis.. Exp. ... Kwak H. J., So J. N., Lee S. J., Kim I., Koh G. Y. Angiopoietin-1 is an apoptosis survival factor for endothelial cells.. FEBS ... Angiopoietin (Ang) 1 and Ang-2 are important regulators of endothelial cell survival. Current models suggest that an increase ...
Angiopoietin 1 antibody for Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)). ... Recommended Angiopoietin 1 Antibody (supplied by: Log in to see ) Antigen Angiopoietin 1 (ANGPT1) Antibodies show synonyms for ... This Angiopoietin 1 antibody is un-conjugated Alternatives Biotin. (19), FITC. (16), HRP. (8), APC. (4), PE. (4), Alkaline ... Target Details Angiopoietin 1 Product Details anti-Angiopoietin 1 Antibody Application Details Handling Images back to top ...
Angiopoietin 1 Polyclonal Antibody. Catalog Number. Pack Size. List Price*. Promo Price*. Quantity. ... Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.. ... Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.. ...
The complex role of angiopoietin-2 in the angiopoietin-tie signaling pathway. Cold Spring Harb Perspect. Med. 2, a006550 (2012 ... K.C.K. and W.C.L. are inventors on patents related to the use of angiopoietin markers, entitled "Angiopoietin-1 and -2 ... Angiopoietin-2 sensitizes endothelial cells to TNF-α and has a crucial role in the induction of inflammation. Nat. Med. 12, 235 ... Angiopoietin-2 functions as a Tie2 agonist in tumor models, where it limits the effects of VEGF inhibition. Cancer Res. 73, 108 ...
  • Angiopoietin 1 is a type of angiopoietin and is encoded by the gene ANGPT1. (wikipedia.org)
  • Immunohistochemical staining of Ang-1 (zeige ANGPT1 ELISA Kits ) was observed in smooth muscle cells, whereas Ang-2 (zeige ANGPT2 ELISA Kits ) was detected in endothelial cells, smooth muscle cells and macrophages. (antikoerper-online.de)
  • Overexpression of angiopoietin 1 (zeige ANGPT1 ELISA Kits ) and metalloproteinases may be the characteristic feature of endometrium with greater potential to transform into endometriotic lesions in the peritoneal cavity. (antikoerper-online.de)
  • Human Lung, Vessel (formalin-fixed, paraffin-embedded) stained with ANGPT1 antibody ABIN218573 at 1:200 followed by biotinylated goat anti-rabbit IgG secondary antibody ABIN481713, alkaline phosphatase-streptavidin and chromogen. (antibodies-online.com)
  • Here, we systematically assessed the expression and function of Angiopoietin-1 ( Angpt1 ) in bone marrow. (elifesciences.org)
  • Angiopoietin-1 antibody LS-C696965 is a Cy3-conjugated rabbit polyclonal antibody to mouse Angiopoietin-1 (ANGPT1). (lsbio.com)
  • Angiopoietin 1, also called ANG1 is a type of angiopoietin and is encoded by the gene ANGPT1. (innov-research.com)
  • This assay has high sensitivity and excellent specificity for detection of Angiopoietin 1 (ANGPT1). (biotecnika.org)
  • No significant cross-reactivity or interference between Angiopoietin 1 (ANGPT1) and analogues was observed. (biotecnika.org)
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiopoietin 1 (ANGPT1) were tested 20 times on one plate, respectively. (biotecnika.org)
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiopoietin 1 (ANGPT1) were tested on 3 different plates, 8 replicates in each plate. (biotecnika.org)
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiopoietin 1 (ANGPT1). (biomatik.com)
  • Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiopoietin 1 (ANGPT1). (biomatik.com)
  • After TMB substrate solution is added, only those wells that contain Angiopoietin 1 (ANGPT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. (biomatik.com)
  • The concentration of Angiopoietin 1 (ANGPT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. (biomatik.com)
  • BACKGROUND AND PURPOSE - : Studies in rodent models suggest that upregulating angiopoietin-1 (Angpt1) improves stroke outcomes. (edu.au)
  • Rhesus Angiopoietin 1 / ANG1 / ANGPT1 Gene ORF cDNA clone expression plasmid, C-Flag tag (CG90025-CF) - available for purchase from ABclonal - allows for easy subcloning of the ORF (pCMV3-C-FLAG Vector) to other plasmid vectors. (abclonal.com)
  • The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). (antibodies-online.com)
  • Angiopoietin-1 (Ang1) and vascular endothelial growth factor (VEGF) play complementary roles in vascular development during embryogenesis. (sciencemag.org)
  • Experiments with cultured mouse endothelial cells revealed that Ang1 did not prevent VEGF from stimulating downstream effectors involved in proliferation (phosphorylation of Akt, extracellular signal-regulated kinases 1 and 2, and focal adhesion kinase) but did prevent disruption of endothelial barrier function by VEGF. (sciencemag.org)
  • Angiopoietin-1 (Ang1) and vascular endothelial growth factor (VEGF) are endothelial cell-specific growth factors. (sciencemag.org)
  • Angiopoietin-1 (Ang1) and vascular endothelial growth factor/vascular permeability factor (VEGF) play separate but essential roles in vascular development during embryogenesis: VEGF acts early in development during formation of the initial vascular plexus, whereas Ang1 acts during the subsequent remodeling of the vasculature into a hierarchical network of mature vessels composed of endothelial and adventitial cells ( 1 ). (sciencemag.org)
  • As with K14-Ang1 mice, the skin of K14-VEGF mice was redder than normal, but, unlike the skin of K14-Ang1 mice, it was also thicker than normal ( Fig. 1 ), with regions of increased cellularity and foci of inflammatory cells. (sciencemag.org)
  • AMG 780 is a mAb that binds to angiopoietin-1 and -2 (Ang1 and Ang2), preventing their binding to the Tie2 receptor to suppress angiogenesis. (aacrjournals.org)
  • The angiopoietin axis is a distinct angiogenic pathway that activates complementary angiogenic processes through binding of the ligands angiopoietin-1 and -2 (Ang1 and Ang2) to the tyrosine kinase receptor Tie2 ( 1 ). (aacrjournals.org)
  • Podocytes produce endothelial factors, including angiopoietin 1 (ang1), and vascular endothelial growth factor (VEGF), whereas GEnC express their respective receptors Tie2 and VEGFR2 in vivo . (asnjournals.org)
  • Full amino acids (20-498) of Ang1 except the secretory signal sequences (amino acid 1-19) for secretion is expressed. (creativebiomart.net)
  • A soluble, stable and potent angiopoietin-1 (Ang1) variant, cartilage oligomeric matrix protein (COMP)-Ang1 has been developed. (oup.com)
  • CsA-induced increases in macrophage infiltration and expression of MCP-1 and ICAM-1 after CsA treatment were significantly reduced by COMP-Ang1. (oup.com)
  • In this study, we used this approach to evaluate the effects of ectopic expression of angiopoietin-1 (Ang1) in normal eyes and those with ocular neovascularization. (elsevier.com)
  • We showed that angiopoietin-1 (ang1) also interacts with integrins on cardiac myocytes (CMs) to increase survival. (core.ac.uk)
  • Notch and Angiopoietin-1 (Ang1)/Tie2 pathways are crucial for vascular maturation and stability. (mdc-berlin.de)
  • TIE-1 and TIE-2 comprise the cell-surface receptors that bind and are activated by the angiopoietins , (Ang1, Ang2, Ang3, Ang4). (wikidoc.org)
  • In humans, three angiopoietins have been identified: Ang1, Ang2, and Ang4 (Ang 3 is the mouse ortholog of human Ang4) [1] . (wikidoc.org)
  • Angiopoietin-1 (Ang1) regulates angiogenesis as a ligand of Tie 2 receptor tyrosine kinase. (elsevier.com)
  • Angiopoietins are proteins with important roles in vascular development and angiogenesis. (wikipedia.org)
  • Angiopoietins, Ang-1, Ang-2, and Ang-3 (mouse)/Ang-4 (human), are natural agonists or antagonists of the Tie-2 receptor tyrosine kinase and are important modulators of angiogenesis. (novusbio.com)
  • Using an endothelial cell-pericyte coculture system and ex vivo neurite sprouting assay, we found that DKK2-mediated juxtacrine signaling in pericyte-endothelial cell interactions promotes angiogenesis and neural regeneration through an angiopoietin-1-Tie2 pathway, rescuing erectile function in diabetic mice. (diabetesjournals.org)
  • Several proteins have been effective in targeting angiogenesis of the penis in diabetic or hypercholesterolemic animal models of ED, including VE growth factor, angiopoietin (Ang)-1, and Ang4 ( 7 - 14 ). (diabetesjournals.org)
  • Human Angiogenesis Panel 1 Detection Antibodies (Cat. (biolegend.com)
  • Human Angiogenesis Panel 1 Standard (Cat. (biolegend.com)
  • These data suggest that imbalances between Ang-1 and -2 that result in a net gain of Ang-2 activity lead to enhanced tumor angiogenesis and growth. (aacrjournals.org)
  • We hypothesized that an imbalance of Ang-1 and -2 in human colon cancer cells leading to a net gain of Ang-2 would lead to the initiation of angiogenesis. (aacrjournals.org)
  • The angiopoietin axis plays an important role in angiogenesis and is distinct from currently approved angiogenesis inhibitors which target the VEGF pathway. (aacrjournals.org)
  • Angiogenesis, the process of new blood vessel formation, is required for tumor growth and metastatic spread and is regulated by a number of different pathways ( 1 ). (aacrjournals.org)
  • However, other growth factors also have a major role in angiogenesis, such as those of the angiopoietin family (e.g. (onlinejacc.org)
  • Our recent demonstration of raised plasma levels of the angiogenic vascular endothelial growth factor (VEGF) in CHF supports the concept that ongoing angiogenesis is probably present in this condition (1) . (onlinejacc.org)
  • More recently, oncology research has identified other growth factors, such as those of the angiopoietin (Ang) family, that appear to have a major role in angiogenesis and metastasis (2-4) . (onlinejacc.org)
  • The latter is responsible for the initiation of angiogenesis through recruitment and proliferation of endothelial cells, a process that Ang-1 potentiates (5) . (onlinejacc.org)
  • More specifically, Ang-1 is a chemotaxin (6) and, in conjunction with VEGF, recruits endothelial cells to initiate and accelerate angiogenesis. (onlinejacc.org)
  • Angiopoietins are proteins with important roles in vascular development and angiogenesis, which bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. (bioaimscientific.com)
  • Angiopoietin-1 (Ang-1) is an endothelium-specific ligand essential for embryonic vascular stabilization, branching morphogenesis, and post-natal angiogenesis, but its contribution to endothelial cell survival has not been completely elucidated. (elsevier.com)
  • The activation of anti-apoptotic pathways mediated by Akt and survivin in endothelial cells may contribute to Ang-1 stabilization of vascular structures during angiogenesis, in vivo. (elsevier.com)
  • Angiopoietin-1 (Ang-1) regulates angiogenesis and reduces vascular permeability and has also been reported to promote monocyte migration in vitro . (aston.ac.uk)
  • Combining nitric oxide (NO)‐mediated increased blood flow with angiopoietin‐1-Tie2 receptor signalling induces arteriolargenesis - the formation of arterioles from capillaries - in a model of physiological angiogenesis. (pubmedcentralcanada.ca)
  • The angiopoietins are protein growth factors required for the formation of blood vessels ( angiogenesis ). (wikidoc.org)
  • The angiopoietins are protein growth factors that regulate angiogenesis , the formation of blood vessels. (wikidoc.org)
  • Since shear stress activates Akt, we determined whether Foxo-1 and the Foxo-1-dependent, angiogenesis-related Ang-2/Tie2-system are influenced by shear stress in endothelial cells. (elsevier.com)
  • In conclusion, Foxo-1 and Ang-2/Tie2 are part of the molecular response to shear stress, which may regulate angiogenesis. (elsevier.com)
  • Our results support a novel paradigm for the ETS factor ESE-1 as a transcriptional mediator of angiogenesis in the setting of inflammation. (qxmd.com)
  • CONCLUSIONS - Our preclinical data demonstrate the efficacy of coadministration of adenoviral VEGF and Ang-1 in increasing angiogenesis and reducing ventricular remodeling in the infarcted diabetic myocardium. (elsevier.com)
  • An increased serum endostatin to AP-1 ratio may reflect impaired angiogenesis that may preclude the development of PAH. (figshare.com)
  • Tek/Tie-2 is an endothelial cell (EC)-specific receptor tyrosine kinase that plays a critical role in angiogenesis via its regulation by the angiopoietin family of growth factor ligands. (elsevier.com)
  • We report the identification of a secreted ligand for TIE2, termed Angiopoietin-1, using a novel expression cloning technique that involves intracellular trapping and detection of the ligand in COS cells. (nih.gov)
  • Although Angiopoietin-1 binds and induces the tyrosine phosphorylation of TIE2, it does not directly promote the growth of cultured endothelial cells. (nih.gov)
  • We have recently cloned a ligand for TIE2, termed Angiopoietin-1. (nih.gov)
  • Here we show that mice engineered to lack Angiopoietin-1 display angiogenic deficits reminiscent of those previously seen in mice lacking TIE2, demonstrating that Angiopoietin-1 is a primary physiologic ligand for TIE2 and that it has critical in vivo angiogenic actions that are distinct from VEGF and that are not reflected in the classic in vitro assays used to characterize VEGF. (nih.gov)
  • To assess the toxicity, pharmacokinetics, tumor vascular response, tumor response, and pharmacodynamics of AMG 780, a mAb designed to inhibit the interaction between angiopoietin-1 and -2 and the Tie2 receptor. (aacrjournals.org)
  • Angiopoietins are a family of growth factors that are ligands for the tyrosine kinase receptor, Tie2. (whiterose.ac.uk)
  • Angiopoietin 1 (Ang-1) is agonistic for Tie2, plays a key role in blood vessel maturation and stability and has been shown to possess anti-inflammatory properties. (whiterose.ac.uk)
  • However, Tie2 expression has been demonstrated on human neutrophils and the observation that neutrophils migrate in response to Ang-1 in vitro has confounded research into its exact role in inflammation as well as its potential use as a therapeutic agent. (whiterose.ac.uk)
  • In addition, recombinant human Ang-1 induced significant chemotaxis of isolated mouse neutrophils in a Tie2- and CD18-dependent manner. (whiterose.ac.uk)
  • Nucleus pulposus cells (NPC) were obtained from 1-year-old bovine coccygeal discs by enzymatic digestion and were sorted for the angiopoietin-1 receptor Tie2. (biomedcentral.com)
  • Angiopoietins were thought to be endothelial cell-specific via the tie2 receptor. (core.ac.uk)
  • We investigated the expression of Ang-1, Ang-2, Tie2, VEGF-A, and VEGF-C genes in bone marrow (BM) mononuclear cells by real-time quantitative PCR (RQ-PCR) in a cohort of 126 patients with newly diagnosed de novo AML and normal marrow donors. (nih.gov)
  • Subgroup analysis showed that Ang-2 expression had prognostic impact on patients with low (but not high) Ang-1 or Tie2 levels, and on patients with high (but not low) VEGF-A or VEGF-C levels. (nih.gov)
  • Angiopoietin-1 (Ang-1), Tie1, and Tie2 mRNA increased during MC differentiation in vitro. (ecu.edu)
  • Myotube formation was enhanced by MC CM and inhibited by EC CM. Ang-1 protein was present in CM from MCs isolated from both the genetically ischemia-susceptible BALB/c and ischemia-resistant C57BL/6 mouse strains, and chimeric Tie2 receptor trapping in situ ablated Ang-1's myogenic effects in vitro. (ecu.edu)
  • Ang-1 or MC CM enhanced myotube formation in a mixed isolate of muscle progenitors as well as a myoblast co-culture with pluripotent mesenchymal cells (10T1/2) and this effect was abrogated by viral expression of the extracellular domain of Tie2 (AdsTie2). (ecu.edu)
  • Furthermore, mesh/tube formation by HUVECs was enhanced by Ang-1 or MC CM and abrogated by Tie2 chimeric receptor trapping. (ecu.edu)
  • Angiopoietin/ Tie2 signaling can reduce the leakiness through changes in endothelial cells. (elsevier.com)
  • Reducing vascular leakage and stabilizing the endothelium through activation of the angiopoietin (ANGPT)-TIE2 receptor tyrosine kinase pathway is a promising therapeutic strategy for vascular diseases. (pnas.org)
  • Signalling through vascular endothelial growth factor (VEGF) receptors and the tyrosine kinase with IgG and EGF domains‐2 (Tie2) receptor by angiopoietins is required in combination with blood flow for the formation of a functional vascular network. (pubmedcentralcanada.ca)
  • The TIE2 receptor ligand, Angiopoietin-1, was isolated by utilizing a novel secretion-trap expression cloning technique. (readabstracts.com)
  • During pregnancy, angiopoietins act complementary to the VEGF system and contribute to endothelial cell survival and the remodeling of vessels. (wikipedia.org)
  • J. S. Gutkind, Angiopoietin-1 prevents VEGF-induced endothelial permeability by sequestering Src through mDia. (sciencemag.org)
  • Vascular endothelial growth factor (VEGF) and the angiopoietins function together during vascular development, with VEGF acting early during vessel formation, and angiopoietin-1 acting later during vessel remodeling, maturation and stabilization. (nih.gov)
  • Here we show that acute administration of angiopoietin-1 does indeed protect adult vasculature from leaking, countering the potentially lethal actions of VEGF and inflammatory agents. (nih.gov)
  • Angiopoietin-1 also acts as an apoptosis survival factor for endothelial cells and this effect is augmented by the presence of VEGF (Kwak et al, 1999). (prospecbio.com)
  • The physiologic roles of Angiopoietin-1 and its receptor are limited to angiogenic processes that occur subsequent to the earlier vasculogenic and angiogenic actions of the VEGF family and their receptors. (prospecbio.com)
  • In addition, at 10 to 12 weeks of age, focal red skin lesions began to develop on the ears of the K14-VEGF mice ( Fig. 1 ). (sciencemag.org)
  • Expression of angiopoietin and VEGF receptors was examined similarly. (asnjournals.org)
  • Angiopoietins (ang) and vascular endothelial growth factors (VEGF) form two groups of growth factors that are of particular importance in directing endothelial cell (EnC) behavior. (asnjournals.org)
  • Citrated plasma was analyzed for Ang-1, Ang-2, tie-2, and VEGF by enzyme-linked immunosorbent assay. (onlinejacc.org)
  • The roles of two members of this family, Ang-1 and Ang-2, are becoming understood and may act alongside VEGF. (onlinejacc.org)
  • Unlike VEGF, Ang-1 and Ang-2 do not have mitogenic/proliferative properties. (onlinejacc.org)
  • In epidemiological studies, raised VEGF, Ang-2, Tie-2 but not Ang-1 have been demonstrated in patients after ACS. (biomedcentral.com)
  • Ang-1 significantly increased plasma MCP-1 and VEGF levels as measured by ELISA. (aston.ac.uk)
  • In healthy adult glomeruli, the proendothelial survival factors vascular endothelial growth factor-A (VEGF-A) and angiopoietin-1 are constitutively expressed in glomerular podocyte epithelia. (umich.edu)
  • We demonstrate that this milieu of vascular growth factors is altered in streptozotocin-induced type 1 diabetic mice, with decreased angiopoietin-1 levels, VEGF-A upregulation, decreased soluble VEGF receptor-1 (VEGFR1), and increased VEGFR2 phosphorylation. (umich.edu)
  • We demonstrate that a fine balance of VEGF and angiopoietin signalling is required for the formation of a functional vascular network. (pubmedcentralcanada.ca)
  • Endogenous VEGF signalling prevents excess neovessel pericyte coverage, and is required for VSMC recruitment during increased nitric oxide‐mediated vasodilatation and angiopoietin signalling (NO-Tie‐mediated arteriogenesis). (pubmedcentralcanada.ca)
  • Endothelial biomarkers, including soluble (s)E-selectin, sP-selectin, angiopoietin 1 and 2 (Ang-1 and Ang-2, respectively), soluble intercellular adhesion molecule 1 (sICAM-1), vascular endothelial growth factor (VEGF), soluble vascular endothelial (VE)-cadherin, and von Willebrand factor (vWf), were measured upon ICU admission. (mdpi.com)
  • We also observed increased phosphorylated mitogen-activated protein kinase-activated protein kinase-2, 2 days after the treatment and increased expression of vascular endothelial growth factor (VEGF), Flk-1, angiopoietin-1 (Ang-1), Tie-2, and survivin, 4 days after treatment in the diabetic animals. (elsevier.com)
  • All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. (wikipedia.org)
  • Angiopoietin-related protein 1 also known as angiopoietin-3 (ANG-3) is a protein that in humans is encoded by the ANGPTL1 gene. (wikipedia.org)
  • The protein encoded by this gene is another member of the angiopoietin family that is widely expressed in adult tissues with mRNA levels highest in highly vascularized tissues. (wikipedia.org)
  • 1999). "Molecular cloning, expression, and characterization of angiopoietin-related protein. (wikipedia.org)
  • We offer Angiopoietin-like Protein 1/ANGPTL1 Peptides and Angiopoietin-like Protein 1/ANGPTL1 Proteins for use in common research applications: Blocking/Neutralizing, Control, ELISA, Protein Array, Western Blot. (novusbio.com)
  • Each Angiopoietin-like Protein 1/ANGPTL1 Peptide and Angiopoietin-like Protein 1/ANGPTL1 Protein is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. (novusbio.com)
  • Our Angiopoietin-like Protein 1/ANGPTL1 Peptides and Angiopoietin-like Protein 1/ANGPTL1 Proteins can be used in a variety of model species: Human. (novusbio.com)
  • Choose from our Angiopoietin-like Protein 1/ANGPTL1 Peptides and Proteins. (novusbio.com)
  • Zusätzlich bieten wir Ihnen Angiopoietin-Like 1 Antikörper (117) und Angiopoietin-Like 1 Proteine (12) und viele weitere Produktgruppen zu diesem Protein an. (antikoerper-online.de)
  • Angiopoietin-like protein (ANGPTL) 4 inactivates LPL and ANGPTL4 expression is controlled by peroxisome proliferator-activated receptors (PPAR). (diva-portal.org)
  • Protein arginine N-methyltransferase 1 is an enzyme that in humans is encoded by the PRMT1 gene.The HRMT1L2 gene encodes a protein arginine methyltransferase that functions as a histone methyltransferase specific for H4. (creativebiomart.net)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Angiopoietin Like Protein 1 (ANGPTL1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. (nrftd.org)
  • Known also as Angiopoietin Like Protein 1 elisa. (nrftd.org)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Angiopoietin Like Protein 1 (ANGPTL1) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species. (nrftd.org)
  • In this study, we investigated a novel role of non-receptor protein tyrosine phosphatase N-2 (PTPN-2) in Ang-1 mediated stabilization of tight junction proteins. (northwestern.edu)
  • Here we show that Ang-1 acting via the Tie 2 receptor induces phosphorylation of the survival serine-threonine kinase, Akt (or protein kinase B). This is associated with up-regulation of the apoptosis inhibitor, survivin, in endothelial cells and protection of endothelium from death-inducing stimuli. (elsevier.com)
  • Exogenous Ang-1 enhanced myogenic (MyoD and Myogenin) mRNA in differentiating myoblasts and increased myosin heavy chain protein. (ecu.edu)
  • Our study demonstrated that promoting stabilization of the BSCB through the acute intraspinal administration of angiopoietin-1, a vascular stabilizing protein, reduced vascular permeability leading to improved functional recovery. (ismrm.org)
  • Mutation of a high-affinity ESE-1 binding site leads to a marked reduction in Ang-1 transactivation by ESE-1, inducibility by inflammatory cytokines, and DNA binding to the ESE-1 protein. (qxmd.com)
  • Background HIV-1-related inflammation is associated with increased levels of biomarkers of vascular adhesion and endothelial activation, and may increase production of the inflammatory protein angiopoietin-2 (ANG-2), an adverse prognostic biomarker in severe systemic infection. (ac.ke)
  • Soluble ICAM-1 and plasma ANG-2 levels decreased at both time-points after ART initiation, with concomitant increases in the beneficial protein ANG-1. (ac.ke)
  • Association of low-density lipoprotein receptor-related protein 1 rs11613352 and angiopoietin-like 3 rs2131925 with hypertension in men-the Tampere. (cdc.gov)
  • Association of low-density lipoprotein receptor-related protein 1 rs11613352 and angiopoietin-like 3 rs2131925 with hypertension might imply a direct effect at the artery wall. (cdc.gov)
  • The structure of Angiopoietin-1 differs from that of known angiogenic factors or other ligands for receptor tyrosine kinases. (nih.gov)
  • To be useful clinically, angiopoietin-1 would have to inhibit leakage when acutely administered to adult vessels, and this action would have to be uncoupled from its profound angiogenic capabilities. (nih.gov)
  • Angiopoietin-1 (Ang-1) is an angiogenic growth factor that promotes the chemotaxis of endothelial cells and facilitates the maturation of new blood vessels. (qxmd.com)
  • Angiopoietin-1 is a ligand for the receptor-like tyrosine kinase designated TIE-2 (Davis et al, 1996). (prospecbio.com)
  • Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. (wikipedia.org)
  • Angiopoietin-1 seems to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. (nih.gov)
  • We also tested the association of concentration of angiopoietin-2 relative to angiopoietin-1 with physiologic and biological markers of activated endothelium. (biomedsearch.com)
  • however the molecular mechanisms related to Ang-1 mediated repair of Tight Junctions (TJs) in brain endothelium still remain elusive. (northwestern.edu)
  • The Tie receptors and their angiopoietin (Ang) ligands have important functions during embryonic vessel assembly and maturation and control adult vascular homeostasis. (nature.com)
  • The Tie receptors and their angiopoietin (Ang) ligands have been identified as the second vascular tissue-specific receptor Tyr kinase system. (nature.com)
  • Figure 3: Structural properties of the Tie receptors and the Angiopoietin ligands. (nature.com)
  • Angiopoietin 1 Polyclonal Antibody by Bioss, Cat. (lucerna-chem.ch)
  • Depletion of resident peritoneal macrophages prior to, or concomitant injections of an anti-CCL4 antibody with MAT.Ang-1 resulted in a significant reduction in neutrophil recruitment. (whiterose.ac.uk)
  • The global Angiopoietin 1 Receptor market is valued at million US$ in 2018 is expected to reach million US$ by the end of 2025, growing at a CAGR of during 2019-2025. (grandresearchstore.com)
  • The global Angiopoietin 1 Receptor market report elucidates the deep summary of existing innovations, specifications, parameter, and production. (editionanalyst.com)
  • The slightest change in the creation profile of Angiopoietin 1 Receptor directs to major modification in the product s prototype, manufacturing method, and research & development stage, these overall factors that are in relation to production are well-explained in the global Angiopoietin 1 Receptor market research report point-to-point and with flowcharts. (editionanalyst.com)
  • Data show that angiopoietin-like proteins zangptl1 and 2 are required for definitive HSPCs formation, and genetically interact with notch (zeige NOTCH1 ELISA Kits ) receptor. (antikoerper-online.de)
  • Two domains characterize the angiopoietin family of proteins: an N-terminal coiled-coil domain that mediates homo-oligomerization, and a C-terminal fibrinogen-like domain that binds Tie-2. (novusbio.com)
  • Angiopoietin_1 (recombinant) (21_496), His_tag recombinant proteins Most stable storage is at - 81 C or lower but some lyophilised proteins can be stored at +4C. (antibody-antibodies.com)
  • Interplay between proximal and distal promoter elements is required for squamous differentiation marker induction in the bronchial epithelium: role for ESE-1, Sp1, and AP-1 proteins. (qxmd.com)
  • However, its expression in close proximity with developing blood vessels implicates Angiopoietin-1 in endothelial developmental processes. (nih.gov)
  • The expression of angiopoietin-1 mRNA is downregulated by PDGF, EGF, IL1-beta, and TGF-beta (Enholm et al, 1997). (prospecbio.com)
  • BACKGROUND Angiopoietin-1 gene expression in human pulmonary hypertensive lungs is directly proportional to increasing pulmonary vascular resistance. (semanticscholar.org)
  • The expression of hVEGF165 and Ang-1 was significantly reduced at day-30 after transduction as seen by RT-PCR. (nus.edu.sg)
  • Administration of C16+Ang-1 and the combined treatment of C16+Ang-1 with L-serine also produced more remarkable inhibitory effects on IL-6 expression compared with L-serine treatment only. (aging-us.com)
  • Adenovirus-mediated systemic expression of Ang-1 induced a significant increase in early atherosclerotic lesion size and monocyte/macrophage accumulation compared with control animals receiving empty vector. (aston.ac.uk)
  • FACS analysis showed that Ang-1 selectively increased inflammatory Gr1 + monocytes in the circulation, while the cell-surface expression of CD11b, which mediates monocyte emigration, was significantly reduced. (aston.ac.uk)
  • We subsequently hypothesized that restoration of angiopoietin-1 expression within glomeruli might ameliorate manifestations of early diabetic glomerulopathy. (umich.edu)
  • The OPN-null microenvironment was sufficient to increase the number of stem cells associated with increased stromal Jagged1 and Angiopoietin-1 expression and reduced primitive hematopoietic cell apoptosis. (rupress.org)
  • In conclusion, our data demonstrated that OGT plays an essential role in regulating heart development through activating expression of cardiomyocyte Angiopoietin-1. (prolekarniky.cz)
  • Expression of Foxo-1 and its target genes p27Kip1 and Ang-2 was decreased under shear stress (6 dyn/cm 2 , 24 h), nuclear exclusion of Foxo-1 by phosphorylation increased. (elsevier.com)
  • No effects on Ang-1 expression were detected. (elsevier.com)
  • Ang-1 expression is up-regulated in response to tumor necrosis factor-alpha (TNF-alpha). (qxmd.com)
  • To begin to elucidate the underlying molecular mechanisms by which Ang-1 gene expression is regulated during inflammation, we isolated 3.2 kb of the Ang-1 promoter that contain regulatory elements sufficient to mediate induction of the promoter in response to TNF-alpha, interleukin-1beta, and endotoxin. (qxmd.com)
  • Transcriptional profiling of cells transiently transfected with an ESE-1 expression vector demonstrates that the endogenous Ang-1 gene is directly inducible by ESE-1. (qxmd.com)
  • Finally, Ang-1 and ESE-1 exhibit a similar and strong expression pattern in the synovium of patients with rheumatoid arthritis. (qxmd.com)
  • Angiopoietin-1 and -2 play modulatory roles by binding a common receptor, the endothelial cell-specific tyrosine kinase receptor tie-2. (onlinejacc.org)
  • Binding of Angiopoietin-1 to its receptor induces tyrosine phosphorylation of the cytoplasmic receptor domain. (prospecbio.com)
  • CD40 induces interleukin-6 gene transcription in dendritic cells: regulation by TRAF2, AP-1, NF-kappa B, AND CBF1. (qxmd.com)
  • The biological activity of the secreted human VEGF165 and Ang-1 was determined by human umbilical vein endothelial cells (HUVEC) proliferation assay, Thymidine [H3] incorporation assay and capillary-like structure formation. (nus.edu.sg)
  • The Quantikine Human Angiopoietin-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Angiopoietin-1 in cell culture supernates, serum, plasma, saliva, and human milk. (novusbio.com)
  • Our Mouse Angiopoietin-1 ELISA Kit is a sandwich ELISA intended for the quantitative determination of mouse Angiopoietin-1 in cell culture supernates, serum and plasma (heparin, EDTA). (innov-research.com)
  • Human Angiopoietin-1 ELISA Kit is for Research Use Only. (bioaimscientific.com)
  • It contains NS0-expressed recombinant human Angiopoietin-1 and has been shown to accurately quantitate the recombinant factor. (novusbio.com)
  • A new animal model for pulmonary hypertension based on the overexpression of a single gene, angiopoietin-1. (semanticscholar.org)
  • Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. (wikipedia.org)
  • Our results demonstrate the ability of muscle and endothelial cell-derived vascular growth factors, particularly Ang-1, to serve as multi-functional stimuli regulating crosstalk between blood vessels and muscle cells during regeneration from ischemic myopathy. (ecu.edu)
  • However, Angiopoietin-1 was expressed in developing blood vessels indicating its role in vascularization. (readabstracts.com)
  • We stably transfected HT29 colon cancer cells with cDNA constructs for Ang-1 or -2 or with vector alone, injected the cells s.c. into nude mice, and assessed tumor growth. (aacrjournals.org)
  • Tumors produced by the Ang-1 transfectants had fewer vessels and lower tumor cell proliferative indices than tumors in the other groups. (aacrjournals.org)
  • Responses to the proinflammatory cytokines interleukin-1 and tumor necrosis factor alpha in cells derived from rheumatoid synovium and other joint tissues involve nuclear factor kappaB-mediated induction of the Ets transcription factor ESE-1. (qxmd.com)
  • The present invention relates to antibodies against angiopoietins 1 and 2, and derivatives of these antibodies. (patentsencyclopedia.com)
  • More specifically, the present invention relates to therapeutic use of the antibodies and fragment thereof which specifically bind to angiopoietins 1 and 2. (patentsencyclopedia.com)
  • Phosphodiesterase type 5 inhibitors enhance the nitric oxide (NO)-cyclic guanosine monophosphate pathway and are currently used as a first-line therapy for ED ( 1 ). (diabetesjournals.org)
  • Construction of a 1.2-Mb sequence-ready contig of chromosome 11q13 encompassing the multiple endocrine neoplasia type 1 (MEN1) gene. (wikipedia.org)
  • OPN (also known as early T cell activation gene-1, or eta-1 ), is a secreted, highly acidic glycoprotein with pleiotropic effects ( 5 - 11 ). (rupress.org)
  • The angiopoietin-2 gene of endothelial cells is up-regulated in hypoxia by a HIF binding site located in its first intron and by the central factors GATA-2 and Ets-1. (qxmd.com)
  • We used a mouse model of peritoneal neutrophilic inflammation to determine if Ang-1 could stimulate neutrophil migration in vivo. (whiterose.ac.uk)
  • McDonald, Donald M. / Pericyte requirement for anti-leak action of angiopoietin-1 and vascular remodeling in sustained inflammation . (elsevier.com)
  • Excess soluble TIE-2 receptors abolish the chemotactic response of endothelial cells toward angiopoietin-1. (prospecbio.com)
  • The soluble extracellular domain modulates signaling by functioning as decoy receptor for angiopoietins. (sdsc.edu)
  • The soluble extracellular domain is functionally active in angiopoietin binding and can modulate the activity of the membrane-bound form by competing for angiopoietins. (sdsc.edu)
  • Objectives The goal of this research was to test the hypothesis that plasma angiopoietin (Ang-1), its soluble receptor tie-2, and Ang-2 levels would be abnormal in patients with acute and chronic congestive heart failure (CHF) when compared with healthy controls. (onlinejacc.org)
  • Furthermore, angiopoietin-1 repletion in diabetic mice increased Tie-2 phosphorylation, elevated soluble VEGFR1, and was paralleled by a decrease in VEGFR2 phosphorylation and increased endothelial nitric oxide synthase Ser(1177) phosphorylation. (umich.edu)
  • Endothelial activation biomarkers including soluble intercellular adhesion molecule-1 (ICAM-1), vascular adhesion molecule-1 (VCAM-1), and E-selectin, and plasma ANG-2 and angiopoietin-1 (ANG-1) were tested in stored plasma samples from 0, 6, and 12 months after ART initiation. (ac.ke)
  • Both soluble ICAM-1 and plasma ANG-2 levels predicted mortality after ART initiation. (ac.ke)
  • Soluble ICAM-1 and plasma ANG-2 levels represent potential biomarkers for adverse outcomes in advanced HIV-1 infection. (ac.ke)
  • In a mouse model of PM, Plasmodium infection of pregnant mice led to decreased angiopoietin-1, increased angiopoietin-2, and an elevated ratio of angiopoietin-2/angiopoietin-1 in the placenta. (wikipedia.org)
  • Diabetic mice receiving a marginal mass of 150 islets pretransduced with Ang-1 vector exhibited near normoglycemia posttransplantation. (diabetesjournals.org)
  • For Mice, Cells, and germplasm please contact us at [email protected] , US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors [email protected] or +1-510-450-7917. (komp.org)
  • C ) In comparison with control littermates, eyes from mice with defective angiopoietin signaling failed to develop a normal SC. (jci.org)
  • Apo-E knockout (Apo-E -/- ) mice fed a western or normal chow diet received a single iv injection of adenovirus encoding Ang-1 or control vector. (aston.ac.uk)
  • Diabetes-induced nephrin phosphorylation was also reduced in mice with angiopoietin-1 repletion. (umich.edu)
  • Angiopoietin-1 also binds to the TIE-1 receptor and this interaction appears to be critical for the development of the right-hand side venous system. (prospecbio.com)
  • The angiopoietin receptors are receptors that bind angiopoietin . (wikidoc.org)
  • They function by binding their physiologic receptors, Tie-1 and Tie-2. (wikidoc.org)
  • Type XII RTKs: TIE family of angiopoietin receptors: tyrosine kinase with immunoglobulin like and EGF like domains 1. (guidetopharmacology.org)
  • Ang-1 binds to the Tie-2 receptor and activates it by inducing phosphorylation and dimerization of the known domains. (aacrjournals.org)
  • Ang-2 also binds to Tie-2 but does not induce phosphorylation and antagonizes the action of Ang-1. (aacrjournals.org)
  • We also found that Ang-1 inhibits thrombin induced tyrosine phosphorylation of Occludin and promote Occludin interaction with Zona Occludens-1 (ZO-1) to stabilize TJs. (northwestern.edu)
  • Transcription factor Foxo-1 can be inactivated via Akt-mediated phosphorylation. (elsevier.com)
  • We previously identified loss-of-function mutations in the angiopoietin (ANGPT) receptor TEK in families with PCG and showed that ANGPT/TEK signaling is essential for SC development. (jci.org)
  • Ang-1, Ang-2, and Ang-3 and Ang-4 are natural agonists or antagonists of the Tie-2 receptor tyrosine kinase. (bioaimscientific.com)
  • MEASUREMENTS AND MAIN RESULTS: Plasma levels of angiopoietin-1 and angiopoietin-2 and of biomarkers of endothelial activation were measured by enzyme-linked immunosorbent assay. (biomedsearch.com)
  • Additionally, plasma samples of Ang-1 and Ang-2 were measured. (arvojournals.org)
  • Higher ANG-2 levels after ART initiation were associated with higher plasma HIV-1 RNA, oral contraceptive pill use, pregnancy, severe malnutrition, and tuberculosis. (ac.ke)
  • Changes in plasma ANG-2 were associated with HIV-1 RNA levels over 12 months of follow-up. (ac.ke)
  • The aim of our study was to evaluate the levels of cytokines (osteopontin and angiopoietins 1 and 2) active in the bone marrow niche during the mobilisation of haematopoietic stem cells for autologous transplantation. (sigmaaldrich.com)
  • Angiopoietin-1 has been shown to counteract cell death by apoptosis in cultured endothelial cells (Holash et al, 1999). (prospecbio.com)
  • Moreover, dominant negative survivin negates the ability of Ang-1 to protect cells from undergoing apoptosis. (elsevier.com)
  • Carlson et al (2001) have shown that Ang-1 binds rather selectively to vitronectin and that Ang-1 can directly support adhesion of human umbilical vein endothelial cells and fibroblasts in a process mediated by integrins. (prospecbio.com)
  • 2001 Nov 1;20(21):5919-5928. (elsevier.com)
  • A naturally occuring antagonist of Angiopoietin-1 binding to TIE-2 is Angiopoietin-2. (prospecbio.com)
  • In a murine mammary carcinoma model, Ang-1 is secreted and then bound to the extracellular matrix (7) where it is ideally situated to mediate cell-to-cell and cell-to-matrix interaction of capillaries (8,9) . (onlinejacc.org)
  • OBJECTIVE: To test the hypothesis that the concentration of angiopoietin-2 relative to angiopoietin-1 may be a useful biological marker of mortality in acute lung injury patients. (biomedsearch.com)
  • 12-14), and have shown that transgenic angiopoietin-1 acts reciprocally as an anti-permeability factor when provided chronically during vessel formation, although it also profoundly affects vascular morphology when thus delivered. (nih.gov)
  • These molecules are widely expressed and have essential and coordinated actions in physiologic regulation of vascular development, maturation, and permeability ( 1 ). (asnjournals.org)
  • By using confocal microscopy and transwell permeability assay, we show that pretreatment of brain endothelial cells with Ang-1 diminish thrombin mediated disruption of TJs and increase in endothelial permeability. (northwestern.edu)
  • He, P 2009, ' Beyond tie-ing up endothelial adhesion: New insights into the action of angiopoietin-1 in regulation of microvessel permeability ', Cardiovascular Research , vol. 83, no. 1, pp. 1-2. (elsevier.com)
  • Synthetic peptide corresponding to Human Angiopoietin 1 + Angiopoietin 2 aa 482-496 (C terminal). (abcam.com)
  • CONCLUSIONS: The ratio of concentration of angiopoietin-2 relative to angiopoietin-1 may be a prognostic biomarker of endothelial activation in acute lung injury patients and, along with pulmonary dead-space fraction, may be useful for risk stratification of acute lung injury patients, particularly in identifying subgroups for future research and therapeutic trials. (biomedsearch.com)
  • Conclusions Biomarkers of endothelial activation decreased after ART initiation in women with advanced HIV-1 infection. (ac.ke)
  • Angiopoietin-1 (ANG-1) and angiopoietin-2 (ANG-2) are major regulators of endothelial activation and integrity. (beds.ac.uk)
  • The aim of this study was to investigate the clinical utility of whole blood angiopoietin (ANG) levels as biomarkers of disease severity in Plasmodium falciparum malaria. (beds.ac.uk)
  • These results suggest that whole blood ANG-1/2 levels are promising clinically informative biomarkers of disease severity in malarial syndromes. (beds.ac.uk)
  • Ratio of angiopoietin-2 to angiopoietin-1 as a predictor of mortality in acute lung injury patients. (biomedsearch.com)
  • The group consisted of patients with multiple myeloma (n=34), lymphoma (n=13) and acute myeloid leukaemia (n=1). (sigmaaldrich.com)