Angiopoietin-2: An angiopoietin that is closely related to ANGIOPOIETIN-1. It binds to the TIE-2 RECEPTOR without receptor stimulation and antagonizes the effect of ANGIOPOIETIN-1. However its antagonistic effect may be limited to cell receptors that occur within the vasculature. Angiopoietin-2 may therefore play a role in down-regulation of BLOOD VESSEL branching and sprouting.Angiopoietin-1: The first to be discovered member of the angiopoietin family. It may play a role in increasing the sprouting and branching of BLOOD VESSELS. Angiopoietin-1 specifically binds to and stimulates the TIE-2 RECEPTOR. Several isoforms of angiopoietin-1 occur due to ALTERNATIVE SPLICING of its mRNA.Receptor, TIE-2: A TIE receptor tyrosine kinase that is found almost exclusively on ENDOTHELIAL CELLS. It is required for both normal embryonic vascular development (NEOVASCULARIZATION, PHYSIOLOGIC) and tumor angiogenesis (NEOVASCULARIZATION, PATHOLOGIC).Angiopoietins: A family of structurally-related angiogenic proteins of approximately 70 kDa in size. They have high specificity for members of the TIE RECEPTOR FAMILY.Receptor, TIE-1: A TIE receptor found predominantly on ENDOTHELIAL CELLS. It is considered essential for vascular development and can form a heterodimer with the TIE-2 RECEPTOR. The TIE-1 receptor may play a role in regulating BLOOD VESSEL stability and maturation.Receptors, TIE: A family of structurally-related tyrosine kinase receptors that are expressed predominantly in ENDOTHELIAL CELLS and are essential for development of BLOOD VESSELS (NEOVASCULARIZATION, PHYSIOLOGIC). The name derives from the fact that they are tyrosine kinases that contain Ig and EGF domains.Angiogenesis Inducing Agents: Agents that induce or stimulate PHYSIOLOGIC ANGIOGENESIS or PATHOLOGIC ANGIOGENESIS.Vascular Endothelial Growth Factor A: The original member of the family of endothelial cell growth factors referred to as VASCULAR ENDOTHELIAL GROWTH FACTORS. Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as "tumor angiogenesis factor" and "vascular permeability factor". Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability it may play a role in stimulating VASODILATION via NITRIC OXIDE-dependent pathways. Alternative splicing of the mRNA for vascular endothelial growth factor A results in several isoforms of the protein being produced.Neovascularization, Physiologic: The development of new BLOOD VESSELS during the restoration of BLOOD CIRCULATION during the healing process.Endothelial Cells: Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.Neovascularization, Pathologic: A pathologic process consisting of the proliferation of blood vessels in abnormal tissues or in abnormal positions.Vascular Endothelial Growth Factors: A family of angiogenic proteins that are closely-related to VASCULAR ENDOTHELIAL GROWTH FACTOR A. They play an important role in the growth and differentiation of vascular as well as lymphatic endothelial cells.Mycoplasma pulmonis: A species of gram-negative bacteria highly pathogenic to RATS and MICE. It is the primary cause of murine respiratory mycoplasmosis.Angiogenic Proteins: Intercellular signaling peptides and proteins that regulate the proliferation of new blood vessels under normal physiological conditions (ANGIOGENESIS, PHYSIOLOGICAL). Aberrant expression of angiogenic proteins during disease states such as tumorigenesis can also result in PATHOLOGICAL ANGIOGENESIS.Endothelial Growth Factors: These growth factors are soluble mitogens secreted by a variety of organs. The factors are a mixture of two single chain polypeptides which have affinity to heparin. Their molecular weight are organ and species dependent. They have mitogenic and chemotactic effects and can stimulate endothelial cells to grow and synthesize DNA. The factors are related to both the basic and acidic FIBROBLAST GROWTH FACTORS but have different amino acid sequences.Receptor Protein-Tyrosine Kinases: A class of cellular receptors that have an intrinsic PROTEIN-TYROSINE KINASE activity.Lymphokines: Soluble protein factors generated by activated lymphocytes that affect other cells, primarily those involved in cellular immunity.Blood Vessels: Any of the tubular vessels conveying the blood (arteries, arterioles, capillaries, venules, and veins).Vascular Endothelial Growth Factor Receptor-2: A 200-230-kDa tyrosine kinase receptor for vascular endothelial growth factors found primarily in endothelial and hematopoietic cells and their precursors. VEGFR-2 is important for vascular and hematopoietic development, and mediates almost all endothelial cell responses to VEGF.Pericytes: Unique slender cells with multiple processes extending along the capillary vessel axis and encircling the vascular wall, also called mural cells. Pericytes are imbedded in the BASEMENT MEMBRANE shared with the ENDOTHELIAL CELLS of the vessel. Pericytes are important in maintaining vessel integrity, angiogenesis, and vascular remodeling.Retinal Neovascularization: Formation of new blood vessels originating from the retinal veins and extending along the inner (vitreal) surface of the retina.Membrane Glycoproteins: Glycoproteins found on the membrane or surface of cells.Capillaries: The minute vessels that connect the arterioles and venules.Endothelium, Vascular: Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.Vascular Endothelial Growth Factor Receptor-1: A 180-kDa VEGF receptor found primarily in endothelial cells that is essential for vasculogenesis and vascular maintenance. It is also known as Flt-1 (fms-like tyrosine kinase receptor-1). A soluble, alternatively spliced isoform of the receptor may serve as a binding protein that regulates the availability of various ligands for VEGF receptor binding and signal transduction.Intercellular Signaling Peptides and Proteins: Regulatory proteins and peptides that are signaling molecules involved in the process of PARACRINE COMMUNICATION. They are generally considered factors that are expressed by one cell and are responded to by receptors on another nearby cell. They are distinguished from HORMONES in that their actions are local rather than distal.Retinal Vessels: The blood vessels which supply and drain the RETINA.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Angiogenesis Inhibitors: Agents and endogenous substances that antagonize or inhibit the development of new blood vessels.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Receptors, Vascular Endothelial Growth Factor: A family of closely related RECEPTOR PROTEIN-TYROSINE KINASES that bind vascular endothelial growth factors. They share a cluster of seven extracellular Ig-like domains which are important for ligand binding. They are highly expressed in vascular endothelial cells and are critical for the physiological and pathological growth, development and maintenance of blood and lymphatic vessels.In Situ Hybridization: A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.Retinal Perforations: Perforations through the whole thickness of the retina including the macula as the result of inflammation, trauma, degeneration, etc. The concept includes retinal breaks, tears, dialyses, and holes.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.Capillary Permeability: The property of blood capillary ENDOTHELIUM that allows for the selective exchange of substances between the blood and surrounding tissues and through membranous barriers such as the BLOOD-AIR BARRIER; BLOOD-AQUEOUS BARRIER; BLOOD-BRAIN BARRIER; BLOOD-NERVE BARRIER; BLOOD-RETINAL BARRIER; and BLOOD-TESTIS BARRIER. Small lipid-soluble molecules such as carbon dioxide and oxygen move freely by diffusion. Water and water-soluble molecules cannot pass through the endothelial walls and are dependent on microscopic pores. These pores show narrow areas (TIGHT JUNCTIONS) which may limit large molecule movement.Reverse Transcriptase Polymerase Chain Reaction: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.Fovea Centralis: An area approximately 1.5 millimeters in diameter within the macula lutea where the retina thins out greatly because of the oblique shifting of all layers except the pigment epithelium layer. It includes the sloping walls of the fovea (clivus) and contains a few rods in its periphery. In its center (foveola) are the cones most adapted to yield high visual acuity, each cone being connected to only one ganglion cell. (Cline et al., Dictionary of Visual Science, 4th ed)Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Diabetic Retinopathy: Disease of the RETINA as a complication of DIABETES MELLITUS. It is characterized by the progressive microvascular complications, such as ANEURYSM, interretinal EDEMA, and intraocular PATHOLOGIC NEOVASCULARIZATION.Cell Proliferation: All of the processes involved in increasing CELL NUMBER including CELL DIVISION.Up-Regulation: A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.Receptors, Growth Factor: Cell surface receptors that bind growth or trophic factors with high affinity, triggering intracellular responses which influence the growth, differentiation, or survival of cells.Umbilical Veins: Venous vessels in the umbilical cord. They carry oxygenated, nutrient-rich blood from the mother to the FETUS via the PLACENTA. In humans, there is normally one umbilical vein.Vitrectomy: Removal of the whole or part of the vitreous body in treating endophthalmitis, diabetic retinopathy, retinal detachment, intraocular foreign bodies, and some types of glaucoma.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Mice, Inbred C57BLVitreous Body: The transparent, semigelatinous substance that fills the cavity behind the CRYSTALLINE LENS of the EYE and in front of the RETINA. It is contained in a thin hyaloid membrane and forms about four fifths of the optic globe.Proto-Oncogene Proteins: Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.Macular Edema: Fluid accumulation in the outer layer of the MACULA LUTEA that results from intraocular or systemic insults. It may develop in a diffuse pattern where the macula appears thickened or it may acquire the characteristic petaloid appearance referred to as cystoid macular edema. Although macular edema may be associated with various underlying conditions, it is most commonly seen following intraocular surgery, venous occlusive disease, DIABETIC RETINOPATHY, and posterior segment inflammatory disease. (From Survey of Ophthalmology 2004; 49(5) 470-90)Enzyme-Linked Immunosorbent Assay: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.Neoplasm Proteins: Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.Disease Models, Animal: Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.Cell Movement: The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.Ischemia: A hypoperfusion of the BLOOD through an organ or tissue caused by a PATHOLOGIC CONSTRICTION or obstruction of its BLOOD VESSELS, or an absence of BLOOD CIRCULATION.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Mice, Transgenic: Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.Fibroblast Growth Factor 2: A single-chain polypeptide growth factor that plays a significant role in the process of WOUND HEALING and is a potent inducer of PHYSIOLOGIC ANGIOGENESIS. Several different forms of the human protein exist ranging from 18-24 kDa in size due to the use of alternative start sites within the fgf-2 gene. It has a 55 percent amino acid residue identity to FIBROBLAST GROWTH FACTOR 1 and has potent heparin-binding activity. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor (FIBROBLAST GROWTH FACTOR 1).Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Phosphorylation: The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.Biological Markers: Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.Cell Survival: The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.Gene Expression Profiling: The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.Mice, Knockout: Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.Dose-Response Relationship, Drug: The relationship between the dose of an administered drug and the response of the organism to the drug.Pregnancy: The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.Rats, Sprague-Dawley: A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.Cell Line, Tumor: A cell line derived from cultured tumor cells.Cell Line: Established cell cultures that have the potential to propagate indefinitely.

Molecular cloning and characterization of a novel angiopoietin family protein, angiopoietin-3. (1/589)

Using homology-based PCR, we have isolated cDNA encoding a novel member (491 amino acids) of the angiopoietin (Ang) family from human adult heart cDNA and have designated it angiopoietin-3 (Ang3). The NH2-terminal and COOH-terminal portions of Ang-3 contain the characteristic coiled-coil domain and fibrinogen-like domain that are conserved in other known Angs. Ang3 has a highly hydrophobic region at the N-terminus (approximately 21 amino acids) that is typical of a signal sequence for protein secretion. Ang3 mRNA is most abundant in adrenal gland, placenta, thyroid gland, heart and small intestine in human adult tissues. Additionally, Ang3 is a secretory protein, but is not a mitogen in endothelial cells.  (+info)

Angiopoietins 3 and 4: diverging gene counterparts in mice and humans. (2/589)

The angiopoietins have recently joined the members of the vascular endothelial growth factor family as the only known growth factors largely specific for vascular endothelium. The angiopoietins include a naturally occurring agonist, angiopoietin-1, as well as a naturally occurring antagonist, angiopoietin-2, both of which act by means of the Tie2 receptor. We now report our attempts to use homology-based cloning approaches to identify new members of the angiopoietin family. These efforts have led to the identification of two new angiopoietins, angiopoietin-3 in mouse and angiopoietin-4 in human; we have also identified several more distantly related sequences that do not seem to be true angiopoietins, in that they do not bind to the Tie receptors. Although angiopoietin-3 and angiopoietin-4 are strikingly more structurally diverged from each other than are the mouse and human versions of angiopoietin-1 and angiopoietin-2, they appear to represent the mouse and human counterparts of the same gene locus, as revealed in our chromosomal localization studies of all of the angiopoietins in mouse and human. The structural divergence of angiopoietin-3 and angiopoietin-4 appears to underlie diverging functions of these counterparts. Angiopoietin-3 and angiopoietin-4 have very different distributions in their respective species, and angiopoietin-3 appears to act as an antagonist, whereas angiopoietin-4 appears to function as an agonist.  (+info)

Angiopoietin-1 is an apoptosis survival factor for endothelial cells. (3/589)

We examined the effect of angiopoietin-1 (Ang1) on apoptosis in human umbilical vein endothelial cells (HUVECs). Ang1 (5-1000 ng/ml) dose-dependently inhibited apoptosis under a serum-deprived state. A significant apoptotic inhibition occurred with as low as 50 ng/ml. Two hundred ng/ml of Ang1 inhibited to approximately 50% of the control apoptotic rates for 96 h. Furthermore, an augmented antiapoptotic effect of Ang1 by the addition of 20 ng/ml vascular endothelial growth factor was observed. This Ang1-induced strong antiapoptotic effect in endothelial cells is a novel and intriguing finding and could be an additional description of Ang1-induced direct biological function.  (+info)

Vessel cooption, regression, and growth in tumors mediated by angiopoietins and VEGF. (4/589)

In contrast with the prevailing view that most tumors and metastases begin as avascular masses, evidence is presented here that a subset of tumors instead initially grows by coopting existing host vessels. This coopted host vasculature does not immediately undergo angiogenesis to support the tumor but instead regresses, leading to a secondarily avascular tumor and massive tumor cell loss. Ultimately, however, the remaining tumor is rescued by robust angiogenesis at the tumor margin. The expression patterns of the angiogenic antagonist angiopoietin-2 and of pro-angiogenic vascular endothelial growth factor (VEGF) suggest that these proteins may be critical regulators of this balance between vascular regression and growth.  (+info)

The transcription factor MEF2C-null mouse exhibits complex vascular malformations and reduced cardiac expression of angiopoietin 1 and VEGF. (5/589)

The MEF2 family of transcription factors has been implicated in transcriptional regulation in a number of different cell types. Targeted deletion of the MEF2C gene, in particular, revealed its importance for early cardiogenesis (Q. Lin et al., 1997, Science 276, 1404-1407). We report here that this deletion also resulted in vascular anomalies characterized by extreme variability in lumen size and defects in remodeling. While primary vascular networks formed in the yolk sac of the mutants, they failed to remodel into more complex vascular structures. Likewise, although the primordia of the dorsal aortae formed normally, anomalies were observed in these vessels later in development. Dorsal and anterior to the heart, the aortae exhibited abnormally small lumens, as did the anterior cardinal veins and intersegmental arteries. In contrast, the dorsal aortae and intersegmental arteries caudal to the heart were grossly enlarged. Cranial vessels were also enlarged and less branched than normal. Endocardiogenesis in the mutant was abnormal with the endothelial cells exhibiting a number of aberrant phenotypes. These endocardial defects were accompanied by a notable reduction in angiopoietin 1 and VEGF mRNA production by the myocardium, indicating that MEF2C is required for myocardial expression of these important endothelial-directed cytokines and thus for correct endocardial morphogenesis.  (+info)

Expressions of angiopoietins and Tie2 in human choroidal neovascular membranes. (6/589)

PURPOSE: To elucidate the potential role of angiopoietins and the Tie2 system in choroidal neovascularization. METHODS: Surgically excised choroidal neovascular membranes (CNVMs) were obtained at vitrectomy from five eyes with age-related macular degeneration, three eyes with idiopathic neovascular maculopathy, and two eyes had degenerative myopia and two eyes had angioid streaks. Light microscopic immunohistochemistry was performed to detect cytokines such as vascular endothelial growth factor (VEGF), Ang1, and Ang2 and cellular components such as retinal pigment epithelial (RPE) cells, macrophages, and endothelial cells. Immunofluorescent double staining using confocal microscopy was performed to identify the cell types that secrete specific cytokines. RESULTS: Ang1 and Ang2 were positive in all surgically excised CNVMs, regardless of the primary disease. Double staining revealed that many of the cytokeratin, CD68 and factor VIII positive cells also had Ang1 and Ang2 immunoreactivities. In contrast to Ang1, Ang2 immunoreactivity tends to be higher in the highly vascularized regions of many CNVMs, and the localization was very similar to that of VEGF staining. Almost all vascular structures had prominent immunoreactivity for Tie2, which was confirmed by double staining for Tie2 and factor VIII. Tie2 immunoreactivity was also observed in the RPE monolayer and in pigmented, polygonal, and fibroblast-like cells in the stroma. CONCLUSIONS: Present findings that Ang2 and VEGF are co-upregulated and that Tie2 is expressed in a variety of cell types in CNVMs further support a crucial role of the interaction between VEGF and Ang2 in pathologic angiogenesis of CNVM formation.  (+info)

Expression of angiopoietin-1, angiopoietin-2, and the Tie-2 receptor tyrosine kinase during mouse kidney maturation. (7/589)

The Tie-2 receptor tyrosine kinase transduces embryonic endothelial differentiation, with Angiopoietin-1 (Ang-1) acting as a stimulatory ligand and Ang-2 postulated to be a naturally occurring inhibitor. Expression of these genes was sought during mouse kidney maturation from the onset of glomerulogenesis (embryonic day 14 [E14]) to the end of nephron formation (2 wk postnatal [P2]), and during medullary maturation into adulthood (P8). Using Northern and slot blotting of RNA extracted from whole organs, these three genes were expressed throughout the experimental period with peak levels at P2 to P3. By in situ hybridization analysis at E18, P1, and P3, Ang-1 mRNA was found to localize to condensing renal mesenchymal cells, proximal tubules, and glomeruli in addition to maturing tubules of the outer medulla. In contrast, Ang-2 transcripts were more spatially restricted, being detected only in differentiating outer medullary tubules and the vasa recta bundle area. Using in situ hybridization and immunohistochemistry, Tie-2 was detected in capillaries of the nephrogenic cortex, glomerular tufts, cortical interstitium, and medulla including vessels in the vasa recta. Using Western blotting of protein extracted from whole organs, Tie-2 protein was detected between E14 and P8 with tyrosine phosphorylated Tie-2 evident from E18. These data are consistent with the hypothesis that Tie-2 has roles in maturation of both glomeruli and vasa rectae.  (+info)

New model of tumor angiogenesis: dynamic balance between vessel regression and growth mediated by angiopoietins and VEGF. (8/589)

Our analyses in several different tumor settings challenge the prevailing view that malignancies and metastases generally initiate as avascular masses that only belatedly induce vascular support. Instead, we find that malignant cells rapidly co-opt existing host vessels to form an initially well-vascularized tumor mass. Paradoxically, the co-opted vasculature does not undergo angiogenesis to support the growing tumor, but instead regresses (perhaps as part of a normal host defense mechanism) via a process that involves disruption of endothelial cell/smooth muscle cell interactions and endothelial cell apoptosis. This vessel regression in turn results in necrosis within the central part of the tumor. However, robust angiogenesis is initiated at the tumor margin, rescuing the surviving tumor and supporting further growth. The expression patterns of Angiopoietin-2 (the natural antagonist for the angiogenic Tie2 receptor) and vascular endothelial growth factor (VEGF) strongly implicate these factors in the above processes. Angiopoietin-2 is highly induced in co-opted vessels, prior to VEGF induction in the adjacent tumor cells, providing perhaps the earliest marker of tumor vasculature and apparently marking the co-opted vessels for regression. Subsequently, VEGF upregulation coincident with Angiopoietin-2 expression at the tumor periphery is associated with robust angiogenesis. Thus, in tumors, Angiopoietin-2 and VEGF seem to reprise the roles they play during vascular remodeling in normal tissues, acting to regulate the previously underappreciated balance between vascular regression and growth.  (+info)

*Vascular endothelial growth factor A

"Vitreous levels of angiopoietin 2 and vascular endothelial growth factor in patients with proliferative diabetic retinopathy". ... 10 (1): 135-47. PMID 7824266. Luttun A, Tjwa M, Moons L, Wu Y, Angelillo-Scherrer A, Liao F, Nagy JA, Hooper A, Priller J, De ... 205 (1): 728-38. doi:10.1006/bbrc.1994.2726. PMID 7999104. Wu LW, Mayo LD, Dunbar JD, Kessler KM, Ozes ON, Warren RS, Donner DB ... 89 (1): 151-63. doi:10.1161/01.cir.89.1.151. PMID 8281642. Sabia PJ, Powers ER, Ragosta M, Sarembock IJ, Burwell LR, Kaul S ( ...

*Angiopoietin-related protein 2

... also known as angiopoietin-like protein 2 is a protein that in humans is encoded by the ANGPTL2 ... angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. ANGPTL2 protein is a secreted glycoprotein ... and characterization of angiopoietin-related protein. angiopoietin-related protein induces endothelial cell sprouting". J Biol ... Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific ...

*Angiopoietin 1

... increased angiopoietin-2, and an elevated ratio of angiopoietin-2/angiopoietin-1 in the placenta. This suggests that ... Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar ... may be associated with a dysregulation in angiopoietins. Increased levels of angiopoietin-1 appear to be associated with a ... Angiopoietin 1 has been shown to interact with TEK tyrosine kinase. Recently, studies in malaria-endemic areas suggest that ...

*Angiopoietin-related protein 1

2005). "Biological characterization of angiopoietin-3 and angiopoietin-4". FASEB J. 18 (11): 1200-8. doi:10.1096/fj.03-1466com ... 1999). "Molecular cloning, expression, and characterization of angiopoietin-related protein. angiopoietin-related protein ... angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. The protein encoded by this gene is another ... "Molecular cloning and characterization of a novel angiopoietin family protein, angiopoietin-3". FEBS Lett. 443 (3): 353-6. doi: ...

*TEK tyrosine kinase

Angiopoietin-1 receptor also known as CD202B (cluster of differentiation 202B) is a protein that in humans is encoded by the ... Master Z, Jones N, Tran J, Jones J, Kerbel RS, Dumont DJ (Nov 2001). "Dok-R plays a pivotal role in angiopoietin-1-dependent ... Also known as TIE2, it is an angiopoietin receptor. The TEK receptor tyrosine kinase is expressed almost exclusively in ... The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations; the TEK ...

*Epithelial-mesenchymal transition

Li, J. J.; Huang, Y. Q.; Basch, R.; Karpatkin, S. (February 2001). "Thrombin induces the release of angiopoietin-1 from ... Angiopoietin-1) and cytokines including the EMT inducer TGF-β. The release of TGF-β by platelets in blood vessels near primary ... 109 (4 Pt 1): 1495-509. doi:10.1083/jcb.109.4.1495. PMC 2115780 . PMID 2677020. Herfs M, Hubert P, Suarez-Carmona M, Reschner A ... 114 (1): 100-109. doi:10.1016/j.jconrel.2006.04.014. ISSN 0168-3659. PMID 16831482. Gershengorn MC, Hardikar AA, Wei C, et al ...

*Pericyte

Also, angiopoietin 2 can act as an antagonist to Tie-2. This destabilizes the endothelial cells, which accounts for less ... Angiopoietin 1 and Tie-2 signaling is essential for maturation and stabilization of endothelial cells. Platelet-derived growth ... Similar to the inhibition of the PDGF pathway, angiopoietin 2 reduces levels of pericytes, leading to diabetic retinopathy. ... "Angiopoietin-2, a Natural Antagonist for Tie2 That Disrupts in vivo Angiogenesis". Science. 277 (5322): 55-60. doi:10.1126/ ...

*GRLF1

Mammoto T, Parikh SM, Mammoto A, Gallagher D, Chan B, Mostoslavsky G, Ingber DE, Sukhatme VP (Aug 2007). "Angiopoietin-1 ... 257 (1): 23-31. doi:10.1016/S0378-1119(00)00387-5. PMID 11054565. Nagase T, Kikuno R, Hattori A, Kondo Y, Okumura K, Ohara O ( ... 23 (1): 94-101. doi:10.1038/nbt1046. PMID 15592455. Barberis D, Casazza A, Sordella R, Corso S, Artigiani S, Settleman J, ... Glucocorticoid receptor DNA-binding factor 1 is a protein that in humans is encoded by the GRLF1 gene. The human glucocorticoid ...

*Vascular endothelial growth factor

The expression of angiopoietin-2 in the absence of VEGF leads to endothelial cell death and vascular regression. Conversely, a ... Makinde T, Murphy RF, Agrawal DK (2007). "Immunomodulatory role of vascular endothelial growth factor and angiopoietin-1 in ... In 1998, neuropilin 1 and neuropilin 2 were shown to act as VEGF receptors. The VEGF family comprises in mammals five members: ... doi:10.1007/978-1-4419-8871-3_13. ISBN 978-1-4020-7704-3. PMID 15015562. Eremina V, Quaggin SE (2004). "The role of VEGF-A in ...

*Thomas N. Sato

... and angiopoietins, that play the key roles in the blood vessel formation. "Laboratory of Biodynamics and Integrative Biology". ... "Requisite role of angiopoietin-1, a ligand for the TIE2 receptor, during embryonic angiogenesis". Cell. 87 (7): 1171-1180. doi: ... "Angiopoietin-2, a Natural Antagonist for Tie2 That Disrupts in vivo Angiogenesis". Science. 277 (5322): 55-60. doi:10.1126/ ... October 1993). "Tie-1 and tie-2 define another class of putative receptor tyrosine kinase genes expressed in early embryonic ...

*Hedgehog signaling pathway

Activation of the Hedgehog pathway leads to an increase in Angiogenic Factors (angiopoietin-1 and angiopoietin-2), Cyclins ( ... Lee, SW; Moskowitz, MA; Sims, JR (2007). "Sonic hedgehog inversely regulates the expression of angiopoietin-1 and angiopoietin- ... 23 (1): 23-34. doi:10.1016/j.ccr.2012.11.017. PMC 3548977 . PMID 23291299. Nakamura, M; Kubo, M; Yanai, K; Mikami, Y; Ikebe, M ... 121 (1): 148-60. doi:10.1172/JCI42874. PMC 3007144 . PMID 21183792. Kim, J.; Lee, J. J.; Kim, J.; Gardner, D.; Beachy, P. A. ( ...

*George Yancopoulos

December 1996). "Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning". Cell. 87 ( ... Retrieved May 1, 2011. "How Nerve Meets Muscle and Begins to Talk". New York Times. May 21, 1996. Retrieved May 1, 2011. Mathew ... 247 (4949 Pt 1): 1446-51. doi:10.1126/science.2321006. PMID 2321006. Boulton TG, Nye SH, Robbins DJ, et al. (May 1991). "ERKs: ... 1 (1): 25-34. doi:10.1016/S1097-2765(00)80004-0. PMID 9659900. DeChiara TM, Kimble RB, Poueymirou WT, et al. (March 2000). " ...

*TNIP2

"ABIN-2 protects endothelial cells from death and has a role in the antiapoptotic effect of angiopoietin-1". Blood. 102 (13): ... 543 (1-3): 55-60. doi:10.1016/S0014-5793(03)00401-0. PMID 12753905. Liu WK, Yen PF, Chien CY, et al. (2004). "The inhibitor ... 36 (1): 40-5. doi:10.1038/ng1285. PMID 14702039. Bouwmeester T, Bauch A, Ruffner H, et al. (2004). "A physical and functional ... 342 (1): 300-9. doi:10.1016/j.bbrc.2006.01.114. PMID 16480954. Greenberg H, Ye X, Wilson D, et al. (2006). "Chronic ...

*ANGPT2

"Genomic structures of the human angiopoietins show polymorphism in angiopoietin-2". Cytogenetics and Cell Genetics. 94 (3-4): ... "Entrez Gene: ANGPT2 angiopoietin 2". Fiedler U, Krissl T, Koidl S, Weiss C, Koblizek T, Deutsch U, Martiny-Baron G, Marmé D, ... Angiopoietin-2 is a protein that in humans is encoded by the ANGPT2 gene. Naturally occurring antagonist for both ANGPT1 and ... Huang YQ, Li JJ, Hu L, Lee M, Karpatkin S (March 2002). "Thrombin induces increased expression and secretion of angiopoietin-2 ...

*DOK2

Master Z, Jones N, Tran J, Jones J, Kerbel RS, Dumont DJ (2001). "Dok-R plays a pivotal role in angiopoietin-1-dependent cell ... 2001). "Dok-R plays a pivotal role in angiopoietin-1-dependent cell migration through recruitment and activation of Pak". EMBO ... 23 (1): 94-101. doi:10.1038/nbt1046. PMID 15592455. Van Slyke P, Coll ML, Master Z, et al. (2005). "Dok-R mediates attenuation ... 105 (6 Pt 1): 1063-70. doi:10.1067/mai.2000.107604. PMID 10856136. Jones N, Dumont DJ (1998). "The Tek/Tie2 receptor signals ...

*MEF2C

... transcription factor MEF2C-null mouse exhibits complex vascular malformations and reduced cardiac expression of angiopoietin 1 ... 19 (1): 21-30. doi:10.1128/mcb.19.1.21. PMC 83862 . PMID 9858528. Wilson-Rawls J, Molkentin JD, Black BL, Olson EN (Apr 1999 ... 180 (1): 227-41. doi:10.1006/dbio.1996.0297. PMID 8948587. Sartorelli V, Huang J, Hamamori Y, Kedes L (Feb 1997). "Molecular ... Maeda T, Gupta MP, Stewart AF (Jun 2002). "TEF-1 and MEF2 transcription factors interact to regulate muscle-specific promoters ...

*GAB2

A paracrine factor, NRG1 β, utilizes GAB2 to activate the ERK and AKT pathways in the heart to produce angiopoietin 1. The C- ... 5 (1): 31-9. doi:10.1093/dnares/5.1.31. PMID 9628581. Wickrema A, Uddin S, Sharma A, Chen F, Alsayed Y, Ahmad S, Sawyer ST, ... 21 (1-2): 72-82. doi:10.1093/emboj/21.1.72. PMC 125816 . PMID 11782427. Pan XL, Ren RJ, Wang G, Tang HD, Chen SD (June 2010). " ... 1 (2): 130-134. PMC 3232456 . PMID 22163099. Zhao C, Ma H, Bossy-Wetzel E, Lipton SA, Zhang Z, Feng GS (September 2003). "GC- ...

*Schlemm's canal

In the combined absence of angiopoietin 1 and angiopoietin 2, Schlemm's canal and episcleral lymphatic vasculature completely ...

*ELF2

2002). "NERF2, a member of the Ets family of transcription factors, is increased in response to hypoxia and angiopoietin-1: a ... 11 (1): 86-96. doi:10.1038/sj.leu.2400516. PMID 9001422. Mao S, Frank RC, Zhang J, et al. (1999). "Functional and physical ... a novel transcription factor related to the Ets factor ELF-1". Mol. Cell. Biol. 16 (9): 5091-106. PMC 231510 . PMID 8756667. " ...

*MTOR inhibitors

Example of these growth factors are angiopoietin 1 (ANG1), ANG 2, basic fibroblast growth factor (bFGF), ephrin-B2, vascular ... Figure 1: Domain structure of the mTOR kinase and components of mTORC1 and mTORC2 Figure 2: The mTOR Signaling Pathway Vilar, E ... ISBN 978-1-84973-126-3. [page needed] Willemsen AE et al. mTOR inhibitor-induced interstitial lung disease in cancer patients: ... 213 (1-2): 25-9. doi:10.1016/s0378-5173(00)00617-7. PMID 11165091. "Orange Book: Approved Drug Products with Therapeutic ...

*Neuroangiogenesis

... such as VEGF and angiopoietin-1. A condition possibly resulting from a reduction in neuroangiogenic factors is Alzheimer's ... 255 (1): 77-98. doi:10.1016/s0012-1606(02)00045-3. PMID 12618135. Park, J. A.; Choi, K. S.; Kim, S. Y.; Kim, K. W. (2003). " ... 2002). "Delayed Up-regulation of Vascular Endothelial Growth Factor and flk-1 after Global Cerebral Ischemia in Mongolian ... "Neurovascular congruence results from a shared patterning mechanism that utilizes Semaphorin3A and Neuropilin-1". Developmental ...

*List of MeSH codes (D12.644)

... angiopoietins MeSH D12.644.276.100.100.100 --- angiopoietin-1 MeSH D12.644.276.100.100.200 --- angiopoietin-2 MeSH D12.644. ... map kinase kinase 1 MeSH D12.644.360.440.200 --- map kinase kinase 2 MeSH D12.644.360.440.300 --- map kinase kinase 3 MeSH ... ADP-ribosylation factor 1 MeSH D12.644.360.525.400 --- rab gtp-binding proteins MeSH D12.644.360.525.400.025 --- rab1 gtp- ... interleukin-1 MeSH D12.644.276.174.500.800 --- tumor necrosis factor-alpha MeSH D12.644.276.174.750 --- tumor necrosis factors ...

*List of MeSH codes (D12.776)

... angiopoietin-1 MeSH D12.776.467.100.100.200 - angiopoietin-2 MeSH D12.776.467.100.450.500 - angiostatins MeSH D12.776.467.100. ... cofilin 1 MeSH D12.776.220.525.212.750 - cofilin 2 MeSH D12.776.220.525.212.875 - destrin MeSH D12.776.220.525.246.500 - actin- ... hiv-1 reverse transcriptase MeSH D12.776.964.970.880.325 - gene products, env MeSH D12.776.964.970.880.325.330 - hiv envelope ... neurofibromin 1 MeSH D12.776.402.150.500.500 - p120 gtpase activating protein MeSH D12.776.402.300.700.500 - ras-GRF1 MeSH ...

*List of MeSH codes (D23)

... angiopoietins MeSH D23.348.479.311.100.100 --- angiopoietin-1 MeSH D23.348.479.311.100.200 --- angiopoietin-2 MeSH D23.348. ... neuregulin-1 MeSH D23.348.740.650 --- proto-oncogene proteins c-sis MeSH D23.348.862.505 --- insulin-like growth factor i MeSH ... thy-1 MeSH D23.050.301.264.965 --- receptors, tumor necrosis factor, type i MeSH D23.050.301.280 --- arrestin MeSH D23.050. ... thy-1 MeSH D23.050.301.264.035.280 --- cd40 ligand MeSH D23.050.301.264.035.281 --- cytokine receptor gp130 MeSH D23.050. ...

*Angiopoietin

To be specific, angiopoietin levels provide an indication for sepsis. Research on angiopoietin-2 has shown that it is involved ... Angiopoietin-2 is elevated in patients with angiosarcoma. Research has shown angiopoietin signaling to be relevant in treating ... Angiopoietins at the US National Library of Medicine Medical Subject Headings (MeSH) "angiopoietin.de: endothelia activation ... In addition, there are a number of proteins that are closely related to ('like') angiopoietins (Angiopoietin-related protein 1 ...

*MECOM

HSCs secrete angiopoietin, and its receptor molecule Tie2 has been implicated in angiogenesis of tumors in both humans and mice ... 13 (1): 183-91. PMID 8700545. Kurokawa M, Mitani K, Irie K, Matsuyama T, Takahashi T, Chiba S, Yazaki Y, Matsumoto K, Hirai H ( ... Perkins AS, Kim JH (Jan 1996). "Zinc fingers 1-7 of EVI1 fail to bind to the GATA motif by itself but require the core site ... 36 (1): 80-9. doi:10.1002/gcc.10144. PMID 12461752. Chi Y, Senyuk V, Chakraborty S, Nucifora G (Dec 2003). "EVI1 promotes cell ...

*Proteases in angiogenesis

... while the angiopoietin receptor Tie-1 facilitates embryonic blood vessel formation. Upon binding of their ligands, Notch-1 and ... ErbB-4 and the angiopoietin receptor Tie-1. Notch-1 signaling is essential for endothelial differentiation, and tumor ... 86 (1): 124-129. PMID 11486997. Bajou, K; Noël, A; Gerard, RD; Masson, V; Brunner, N; Holst-Hansen, C; Skobe, M; Fusenig, NE; ... Unexpectedly, when PAI-1 deficient mice were challenged with cancer cells on a collagenous matrix, angiogenesis and vascular ...
To test the hypothesis that the concentration of angiopoietin-2 relative to angiopoietin-1 may be a useful biological marker of mortality in acute lung injury patients. We also tested the association of concentration of angiopoietin-2 relative to ang
Angiopoietin 1 and Angiopoietin 2 are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie 2. Angiopoietin 2 is only 60% homologous with Angiopoietin 1. Angiopoietin-2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation. Angiopoietin 1 binding to Tie 2 causes phosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thus blocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus. ...
The angiopoietin/Tie (ANG/Tie) receptor system controls developmental and tumor angiogenesis, inflammatory vascular remodeling, and vessel leakage. ANG1 is a Tie2 agonist that promotes vascular stabilization in inflammation and sepsis, whereas ANG2 is a context-dependent Tie2 agonist or antagonist. A limited understanding of ANG signaling mechanisms and the orphan receptor Tie1 has hindered development of ANG/Tie-targeted therapeutics. Here, we determined that both ANG1 and ANG2 binding to Tie2 increases Tie1-Tie2 interactions in a β1 integrin-dependent manner and that Tie1 regulates ANG-induced Tie2 trafficking in endothelial cells. Endothelial Tie1 was essential for the agonist activity of ANG1 and autocrine ANG2. Deletion of endothelial ...
Background - Angiopoietin-1 (Ang1) and vascular endothelial growth factor (VEGF) are endothelial cell-specific growth factors. In the current study, we tested the hypothesis that co-expression of AngI and VEGF could have an effect on development of leakage-resistant vessels.. Methods and results - Expression plasmids, pcD2 (control plasmid), pcD2/Ang1, pcD2/VEGF(121), or pcD2/AngI + pcD2/VEGF(121), were injected intramuscularly into an ischaemic hindlimb rat model, followed by electroporation. Collateral vessel development and skeletal muscle atrophy were assessed before and 7, 14, 28 days after treatment. Capillary density was significantly increased in the rats transfected with Ang1 or VEGF compared with that in the rats transfected with pcD2 alone (P < 0.05). Rats transfected with AngI + VEGF had the highest capillary density (P < 0.05). The mean perimeter ratio of ligated hindlimb to non-ligated hindlimb was lower with pcD2 treatment rats compared with that in the rats transfected with Ang1, ...
Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning. The anticoagulation factor protein S and its relative, Gas6, are ligands for the Tyro 3/Axl family of receptor tyrosine kinases
4652 Communications between endothelial cells (ECs) and mural cells is critical in vascular maturation. Genetic studies suggest that angiopoietin/Tie-2 signaling may play a role in the recruitment of pericytes or smooth muscle cells (SMCs) during vascular maturation. However, the molecular mechanism is unclear. We used microarray technology to analyze genes regulated by angiopoietin-1 (Ang1), an agonist ligand for Tie2, in endothelial cells (ECs). We observed that hepatocyte growth factor (HGF), a mediator of mural cell motility, was upregulated by Ang1 stimulation. We confirmed this finding by Northern blotting and Western blotting in cultured vascular endothelial cells. Furthermore, stimulation of ECs with Ang1 increased SMC migration toward endothelial cells in a co-culture assay. Addition of a neutralizing anti-HGF antibody inhibited the Ang1-induced SMC recruitment, indicating that the induction of SMC migration by Ang1 was due to the increase of HGF. Very interestingly, angiopoietin-2 ...
The results of the present study indicate that TAK1 acts as an AMPKα1 kinase in endothelial cells and positively regulates VEGF-induced, TNF-α-induced, and IL-1β-induced angiogenesis by increasing the expression of the antioxidative mitochondrial enzyme, SOD2. Indeed, superoxide production and the mitochondrial membrane potential were reduced in the absence of TAK1, and the impaired endothelial cell sprouting from aortic rings from TAKΔEC and AMPKα1ΔEC mice was rescued in the presence of PEG-SOD.. The global deletion of TAK1 impairs embryonic development in C. elegans,23 zebrafish, and mice.13 Both the global and endothelial-specific deletions of TAK1 result in vascular malformation, dilated capillaries, and defects in vascular stabilization,14 and correlate well with the phenotype of endothelial cell-specific TGF-β type II receptor conditional mutants.24 In addition to its role in vascular development, TAK1 has also been implicated in the TGF-β-induced regulation of matrix ...
How do reduced levels of ALK-1 or endoglin lead to vascular lesions? During development, both proteins are expressed most prominently in vascular endothelium.49,50 Both are downregulated in adulthood but reinduced during vessel repair.43,49,51,52 ALK-1 and endoglin have multiple actions on developing blood vessels, but their functions in vascular stabilization seem most central to disease etiology. ALK-1 or endoglin knockout mice establish a primary capillary plexus but fail to recruit pericytes and form a secondary plexus.53,54 Mice with mutations in just one copy of either gene establish relatively normal mature vasculature but have small-vessel malformations and loss of vascular smooth muscle.45,46 Defects in these mice resemble those in human HHT, in which dissociation of vascular smooth muscle cells is an early step,30 and sporadic brain AVM, in which there is reduced pericyte coverage of the perinidal capillaries.55. Although ALK-1 and endoglin deficiencies manifest first histologically as ...
In the circulatory system, while larger features of the vasculature such as arteries and veins appear to be statically positioned for years of use, finer features in the human body and in biology relates to a continuous branching out of a circulatory network that is required for tissue growth. This process is related to biochemical pathways and is vital to be understood so that issues in health and disease can be better addressed. Angiogenesis is the formation of new blood vessels from existing vessels. A functional vascular network is established by balanced angiogenesis processes. While the activation of angiogenesis extends vascular networks crucial for tissue growth, angiogenic vessels should be stabilized to undertake necessary vascular functions. The Notch pathway is fundamental for allowing for vascular stabilization. It acts by preventing excessive angiogenesis. However, how this signaling pathway that is conserved in various biological processes can induce changes that are ...
In this study we found that: (1) LS inhibits tubule formation in HUVECs and HMECs, but not BAECs, however CM from laminar sheared BAECs can inhibit tubule formation of HUVECs, (2) Preconditioning with LS inhibits migration in HUVECs and BAECs, whereas OS does not inhibit migration and this is mediated through secreted protein, (3) Ang2 is downregulated by LS both at the gene and protein level in vitro, (4) Ang2 is downregulated at sites of LS and upregulated at sites of OS in vivo, (5) Knockdown of Ang2 inhibits OS-mediated tubule formation and migration, and (6) Addition of recombinant Ang2 partially rescues LS-inhibited tubule formation. Collectively, these findings suggest that Ang2 secreted by ECs in response to OS, acts as a promigratory and proangiogenic molecule that could play an important role in diseases with altered shear stress.. Fluid shear stress is thought to play a role in angiogenesis and arteriogenesis. In angiogenesis, endothelial proliferation and migration are 2 important ...
Sigma-Aldrich offers abstracts and full-text articles by [K Teichert-Kuliszewska, P C Maisonpierre, N Jones, A I Campbell, Z Master, M P Bendeck, K Alitalo, D J Dumont, G D Yancopoulos, D J Stewart].
The interaction between fluid status and angiopoietin-2 in adverse renal outcomes of chronic kidney disease. Yi-Chun Tsai, Yi-Wen Chiu, Hung-Tien Kuo, Jia-Jung Lee, Su-Chu Lee, Tzu-Hui Chen, Ming-Yen Lin, Shang-Jyh Hwang, Mei-Chuan Kuo, Ya-Ling Hsu, Hung-Chun Chen. Journal article , Research Article , Published 23 Mar 2017 , PLOS ONE ...
Patagonian opossum: Lestodelphys halli a small insectivorous and carnivorous marsupial (family Didelphidae, subfamily Didelphinae) found only in south-central Argentina, occurring farther south than...
Looking for online definition of angiopoietin-2a in the Medical Dictionary? angiopoietin-2a explanation free. What is angiopoietin-2a? Meaning of angiopoietin-2a medical term. What does angiopoietin-2a mean?
Angiopoietins are a family of growth factors that are ligands for the tyrosine kinase receptor, Tie2. Angiopoietin 1 (Ang-1) is agonistic for Tie2, plays a key role in blood vessel maturation and stability and has been shown to possess anti-inflammatory properties. However, Tie2 expression has been demonstrated on human neutrophils and the observation that neutrophils migrate in response to Ang-1 in vitro has confounded research into its exact role in inflammation as well as its potential use as a therapeutic agent. We used a mouse model of peritoneal neutrophilic inflammation to determine if Ang-1 could stimulate neutrophil migration in vivo. Tie2 expression was demonstrated on mouse neutrophils. In addition, recombinant human Ang-1 induced significant chemotaxis of isolated mouse neutrophils in a Tie2- and CD18-dependent manner. Subsequently, co-immunoprecipitation of Ang-1 and CD18 demonstrated their interaction. Intraperitoneal injection of an engineered angiopoietin-1, MAT.Ang-1, induced ...
TY - JOUR. T1 - Coadministration of adenoviral vascular endothelial growth factor and angiopoietin-1 enhances vascularization and reduces ventricular remodeling in the infarcted myocardium of type 1 diabetic rats. AU - Mathews Samuel, Samson. AU - Akita, Yuzo. AU - Paul, Debayon. AU - Thirunavukkarasu, Mahesh. AU - Zhan, Lijun. AU - Sudhakaran, Perumana R.. AU - Li, Chuanfu. AU - Maulik, Nilanjana. PY - 2010/1/1. Y1 - 2010/1/1. N2 - OBJECTIVE - Hyperglycemia impairs angiogenesis in response to ischemia, leading to ventricular remodeling. Although the effects of overexpressing angiogenic growth factors have been studied in inducing angiogenesis, the formation of functional vessels remains a challenge. The present study evaluates the reversal of diabetes-mediated impairment of angiogenesis in the infarcted diabetic rat myocardium by proangiogenic gene therapy. RESEARCH DESIGN AND METHODS - Ad.VEGF and Ad.Ang1 were intramyocardially administered in combination immediately after myocardial ...
Angiopoietin-1 (Ang1) and vascular endothelial growth factor (VEGF) play complementary roles in vascular development during embryogenesis. In the adult, VEGF signaling increases vascular permeability and Ang1 blocks this VEGF-mediated response. Gavard et al. provide evidence that this function of Ang1 is mediated by its actions on the endothelial cells, preventing them from responding to VEGF signaling that triggers disruption of adherens junctions. When injected subcutaneously in mice, Ang1 prevented dermal vascular permeability in response to VEGF injection (acute in vivo vascular permeability model). Experiments with cultured mouse endothelial cells revealed that Ang1 did not prevent VEGF from stimulating downstream effectors involved in proliferation (phosphorylation of Akt, extracellular signal-regulated kinases 1 and 2, and focal adhesion kinase) but did prevent disruption of endothelial barrier function by VEGF. Preexposure of endothelial cell cultures to Ang1 inhibited the ability of ...
Coronary heart disease (CHD) is characterized by inflammatory process and endothelial dysfunction. To investigate angiopoietin-2 (Ang-2) profiles, we evaluated serum Ang-2 levels in different types of CHD in 166 subjects. Ang-2 was measured by enzyme
To the Editor:. Vascular endothelial growth factor (VEGF) is a potent angiogenic factor and plays a key role in postnatal angiogenesis in human pathophysiology, including cardiovascular disease. The activation of the renin-angiotensin system has been similarly implicated in the cardiovascular pathophysiology. Indeed, an interaction between the renin-angiotensin system and VEGF has been established. It is against this background that we read with great interest the report by Zhao and colleagues1 on the central role of VEGF on angiotensin II (Ang II)-mediated vascular inflammation.. However, it is now increasingly evident that molecules traditionally regarded as angiogenic growth factors have effects that extend beyond these conventionally defined roles. In particular, angiopoietin-1 and its natural antagonist angiopoietin-2, in conjunction with VEGF, are key mediators of angiogenesis and are involved in the regulation of vascular inflammation and integrity.2 Angiopoietin-1 induces the recruitment ...
This study demonstrates the distinct spatiotemporal expression profile of Angpt2 after MI and I/R, which, to date, has been obscure due to a lack of reliable antibodies (54). Using our recently developed anti-Angpt2 antibody (23), which is highly sensitive and specific to Angpt2, we demonstrate distinct expressions of Angpt2 on the ECs and proinflammatory macrophages after ischemia. This prompted us to further investigate the role of Angpt2 in postischemic cardiovascular remodeling. We recently reported that the FOXO1/Angpt2 axis suppresses the endothelial PI3K/Akt-signaling pathway, the main downstream pathway for Tie2 that plays a vital role in maintaining vascular integrity, inducing vascular destabilization in a positive feedback manner (25), and that it is central to the pathogenesis of sepsis (23), diabetic retinopathy (25), and tumor vessel destabilization (38). This study provides additional insights into the serious detrimental effects of the FOXO1/Angpt2 axis in exacerbating cardiac ...
Sphingosine-1-phosphate (S1P) is the endogenous ligand for the sphingosine-1-phophate receptors (S1P1-5) and triggers a number of cellular responses through their stimulation. S1P and its interaction with the S1P receptors play a significant role in a variety of biological processes including vascular stabilization, heart development, lymphocyte homing, and cancer angiogenesis. Agonism of S1P1, especially, has been shown to play an important role in lymphocyte trafficking from the thymus and secondary lymphoid organs, inducing immunosuppression, which has been established as a novel mechanism of treatment for immune diseases and vascular diseases. Sphingosine-1 -phosphate (SlP) has been demonstrated to induce many cellular effects, including those that result in platelet aggregation, cell proliferation, cell morphology, tumor cell invasion, endothelial cell and leukocyte chemotaxis, endothelial cell in vitro angiogenesis, and lymphocyte trafficking. SlP receptors are therefore good targets for a ...
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Sigma-Aldrich offers abstracts and full-text articles by [Qiupeng Zheng, Jing Du, Zhaofeng Zhang, Jianhua Xu, Lingyuan Fu, Yunlei Cao, Xianliang Huang, Lingli Guo].
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infection by acting as a Tie2 antagonist, which led to p-Tie2 suppression, forkhead box O1 (FOXO1) activation, increased ANG2 expression, and vessel leakiness. These changes were exaggerated by anti-Tie2 antibody, inhibition of PI3K signaling, or ANG2 overexpression and were reduced by anti-ANG2 antibody or exogenous ANG1. In contrast, under pathogen-free conditions, ANG2 drove vascular remodeling by acting as an agonist, promoting high p-Tie2, low FOXO1 activation, and no leakage. Tie1 activation was strong under pathogen-free conditions, but infection or TNF-α led to Tie1 inactivation by ectodomain cleavage and promoted the Tie2 antagonist action of ANG2. Together, these data indicate that ANG2 activation of Tie2 supports stable enlargement of normal nonleaky vessels, but reduction of Tie1 in inflammation leads to ANG2 antagonism of Tie2 and initiates a positive feedback loop wherein FOXO1-driven ANG2 expression promotes vascular remodeling and leakage.. ...
Buy our Recombinant human Angiopoietin 1 protein. Ab69492 is an active full length protein produced in HeLa cells and has been validated in FuncS, SDS-PAGE…
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Polyclonal antibody for Angiopoietin 2/ANGPT2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. Angiopoietin 2/ANGPT2 information: Molecular Weight: 56919 MW; Subcellular Localization: Secreted.
Affiliation:名古屋市立大学,大学院医学研究科,講師, Research Field:Ophthalmology, Keywords:脈絡膜新生血管,炎症,加齢黄斑変性,ペリサイト,細胞生物学,リポフスチン,Angiopoietin-2,マウス,発現抑制,脂質, # of Research Projects:5, # of Research Products:33, Ongoing Project:加齢黄斑変性の病態におけるブルッフ膜への加齢性沈着脂質の役割の解明
Angiopoietin 2 antibody (angiopoietin 2) for ELISA, IHC-P, WB. Anti-Angiopoietin 2 pAb (GTX10601) is tested in Human samples. 100% Ab-Assurance.
Angiopoietin 1兔多克隆抗体(ab94684)可与小鼠, 人样本反应并经WB, IHC实验严格验证并得到1个独立的用户反馈。所有产品均提供质保服务,中国75%以上现货。
Published data on the role of the Ang/tie-2 system in cardiovascular diseases are limited. Most of our knowledge regarding the angiopoietins has come from oncology studies where angiogenesis is a prerequisite for tumor growth and metastasis. Indeed, Ang-2 has been shown to be a marker of a poor prognosis in breast cancer and non-small cell lung cancer (4,21). In the present paper, we describe a new assay for Ang-1 and, in addition, have demonstrated (for the first time in the literature) very abnormal levels of Ang-2 and tie-2, but normal Ang-1, in CHF. Vascular endothelial growth factor was marginally raised in the patients, suggesting that Ang-2 may be more relevant to the pathophysiology of this disease. In addition, there was a significant correlation between Ang-2 and tie-2.. That Ang-1 is not raised in CHF is consistent with currently held views that its secretion is not stimulated by hypoxia, as demonstrated in animal studies (13,14,22). Angiopoietin-1 has been shown to have antiapoptotic ...
Background: Microcirculatory dysfunction is associated with multiple organ failure and unfavorable patient outcome. We investigated whether therapeutically targeting the endothelial angiopoietin/Tie2 system preserves microvascular integrity during hemorrhagic shock. Methods: Rats were treated with the angiopoietin-1 mimetic vasculotide and subjected to hemorrhagic shock and fluid resuscitation. Microcirculatory perfusion and leakage were assessed with intravital microscopy (n = 7 per group) and Evans blue dye extravasation (n = 8 per group), respectively. The angiopoietin/Tie2 system was studied at protein and RNA level in plasma, kidneys, and lungs. Results: Hemorrhagic shock significantly reduced continuously perfused capillaries (7 ± 2 vs. 11 ± 2) and increased nonperfused vessels (9 ± 3 vs. 5 ± 2) during hemorrhagic shock, which could not be restored by fluid resuscitation. Hemorrhagic shock increased circulating angiopoietin-2 and soluble Tie2 significantly, which associated with ...
Angiopoietin-related protein 2 also known as angiopoietin-like protein 2 is a protein that in humans is encoded by the ANGPTL2 gene. Angiopoietin-like protein 2 maintains tissue homeostasis by promoting adaptive inflammation and subsequent tissue reconstruction, whereas an excess of ANGPTL2 activation induced by prolonged stress promotes the breakdown of tissue homeostasis due to chronic inflammation, promoting the development of metabolic diseases. ANGPTL2 has a role also in angiogenesis, in tissue repair, in obesity, in atherosclerotic diseases and finally in cancerogenesis. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. ANGPTL2 protein is a secreted glycoprotein with homology to the angiopoietins and may exert a function on endothelial cells through autocrine or paracrine action. GRCh38: ...
OBJECTIVES: To examine the potential role of the angiogenic growth factor angiopoietin-1 (Ang-1) in inflammatory arthritis. METHODS: Eighteen synovial tissue samples were obtained from 17 patients with a clinical diagnosis of rheumatoid arthritis (RA) and compared with six synovial tissue samples from six patients with osteoarthritis (OA). Ang-1 expression in synovial tissues was determined by immunohistochemistry and in situ hybridisation. Ang-1 mRNA and protein expression were also examined by northern blot analysis and enzyme linked immunosorbent assay (ELISA) in cultured synovial fibroblasts and human umbilical vein endothelial cells (HUVECs) before and after treatment with tumour necrosis factor (TNF)alpha. RESULTS: Ang-1 protein expression was detected by immunohistochemistry in 16/18 RA synovial tissue samples. Ang-1 protein was frequently observed in the synovial lining layer and in cells within the sublining synovial tissue, in both perivascular areas and in areas remote from vessels. In
Background. In sepsis and various other inflammatory conditions, elevated circulating levels of angiopoietin-2 (Ang2) are detected, but the precise functional role of Ang2 in these conditions is not well understood. Here, we investigated the contribution of Ang2 to the inflammatory response and renal function impairment in a mouse model of endotoxaemia.. Methods. Ang2-deficient mice and wild-type littermates were challenged with lipopolysaccharide [LPS; 1500 EU/g, intraperitoneal (i.p.)]. In additional experiments, wild-type C57Bl/6 mice were depleted of circulating neutrophils by antibody treatment (NIMPR14) prior to LPS challenge to study the role of neutrophils in regulating LPS-induced cytokine release. After 8 or 24 h of LPS challenge, the mice were sacrificed and organs were harvested. Quantitative reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay were performed for endothelial adhesion molecules (P-selectin, E-selectin, VCAM-1 and ICAM-1) and plasma ...
Hyperoxia-induced acute lung injury (HALI) is a key contributor to the pathogenesis of bronchopulmonary dysplasia (BPD) in neonates, for which no specific preventive or therapeutic agent is available. Here we show that lung micro-RNA (miR)-34a levels are significantly increased in lungs of neonatal mice exposed to hyperoxia. Deletion or inhibition of miR-34a improves the pulmonary phenotype and BPD-associated pulmonary arterial hypertension (PAH) in BPD mouse models, which, conversely, is worsened by miR-34a overexpression. Administration of angiopoietin-1, which is one of the downstream targets of miR34a, is able to ameliorate the BPD pulmonary and PAH phenotypes. Using three independent cohorts of human samples, we show that miR-34a expression is increased in type 2 alveolar epithelial cells in neonates with respiratory distress syndrome and BPD. Our data suggest that pharmacologic miR-34a inhibition may be a therapeutic option to prevent or ameliorate HALI/BPD in neonates.
Mice. Specific pathogen-free (SPF) C57BL/6J mice (catalog 000664), Tie2-GFP mice (catalog 003658), and UBC-Cre-ERT2 mice (catalog 007001) were purchased from the Jackson Laboratory. Angpt1fl/fl mice were a gift from Yoshikazu Nakaoka (Osaka University, Osaka, Japan), Angpt1-GFP mice were a gift from Sean J. Morrison (University of Texas Southwestern, Dallas, Texas, USA), Angpt2-lacZ mice were a gift from Nicholas Gale (Regeneron Pharmaceuticals), and Vegfr2fl/fl mice were a gift from Masanori Hirashima (Kobe University, Kobe, Japan). Prox1-GFP (30), Angpt1-GFP (33), Prox1fl/fl (39), Tie2fl/fl (35), Angpt1fl/fl (36, 37), Angpt2fl/fl (38), Vegfr2fl/fl (55), and Angpt2-lacZ (22) mice were transferred and bred in our SPF animal facility at KAIST. To deplete Prox1, Tie2, or Vegfr2 genes specifically in ECs, VE-cadherin-Cre-ERT2 mice (34) were mated with either Prox1fl/fl, Tie2fl/fl, or Vegfr2fl/fl mice to obtain EC-specific Prox1-, Tie2-, or Vegfr2-deleted mice, respectively, in a tamoxifen-dependent ...
Mice. Specific pathogen-free (SPF) C57BL/6J mice (catalog 000664), Tie2-GFP mice (catalog 003658), and UBC-Cre-ERT2 mice (catalog 007001) were purchased from the Jackson Laboratory. Angpt1fl/fl mice were a gift from Yoshikazu Nakaoka (Osaka University, Osaka, Japan), Angpt1-GFP mice were a gift from Sean J. Morrison (University of Texas Southwestern, Dallas, Texas, USA), Angpt2-lacZ mice were a gift from Nicholas Gale (Regeneron Pharmaceuticals), and Vegfr2fl/fl mice were a gift from Masanori Hirashima (Kobe University, Kobe, Japan). Prox1-GFP (30), Angpt1-GFP (33), Prox1fl/fl (39), Tie2fl/fl (35), Angpt1fl/fl (36, 37), Angpt2fl/fl (38), Vegfr2fl/fl (55), and Angpt2-lacZ (22) mice were transferred and bred in our SPF animal facility at KAIST. To deplete Prox1, Tie2, or Vegfr2 genes specifically in ECs, VE-cadherin-Cre-ERT2 mice (34) were mated with either Prox1fl/fl, Tie2fl/fl, or Vegfr2fl/fl mice to obtain EC-specific Prox1-, Tie2-, or Vegfr2-deleted mice, respectively, in a tamoxifen-dependent ...
Human platelet lysate (PL) is a cost-effective and human source of autologous multiple and potent pro-angiogenic factors, such as vascular endothelial growth factor A (VEGF A), fibroblast growth factor b (FGF b) and angiopoietin-1. Nanocoatings previously characterized were prepared by layer-by-layer assembling incorporating PL with marine-origin polysaccharides and were shown to activate human umbilical vein endothelial cells (HUVECs). Within 20 h of incubation, the more sulfated coatings induced the HUVECS to the form tube-like structures accompanied by an increased expression of angiogenic-associated genes, such as angiopoietin-1 and VEGF A. This may be a cost-effective approach to modify 2D/3D constructs to instruct angiogenic cells towards the formation of neo-vascularization, driven by multiple and synergistic stimulations from the PL combined with sulfated polysaccharides.. ...
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Ngok, S., R. Geyer, S. Agrawal, A. Kourtidis, L. J. Lewis-Tuffin, K. L. Moodie3, D Huveldt, C. Wu, M. Liu, R. Marx, J. M. Baraban, P. Storz, A. Horowitz*, and P. Z. Anastasiadis*. VEGF and Angiopoietin-1 exert opposing effects on cell junctions by regulating the Rho GEF Syx. J. Cell Biol., 199:1103-1115, 2012 (* - co senior authors). Discussed by the JCB editors in "In this Issue" and in Sci. Signal. editorial (Converging on Syx, Vol. 6, Issue 257, p. ec9); recommended by Faculty of 1000 (http://f1000.com/prime/717978940), PubMed ID: 23253477; PubMed Central ID: PMC3529520.. We report a new molecular mechanism that may account in part for the opposite effects of VEGF and Angiopoietin-1 on vascular permeability. VEGF triggers endothelial cell junction disassembly, whereas Ang1 stabilizes them. We found that the key to these effects is the location of the RhoA-specific GEF Syx: in quiescent or Ang1-treated cells, Syx is anchored to a junctional protein complex where it stabilizes the actin fibers ...
Purified Recombinant Human ANGPT1/COMP protein, FLAG-tagged from Creative Biomart. Recombinant Human ANGPT1/COMP protein, FLAG-tagged can be used for research.
401366DNAHomo sapiens 1gaggtgcagc tggtggagtc tgggggaggc ttggtccagc ctggggggtc cctgagactc 60tcctgtgcag tctctggatt cacctttagt agctattgga tgagctgggt ccgccaggct 120ccagggaagg ggctggagtg ggtggccaac ataaagcaag atggaagtga gaaatactat 180gtggactctg tgaagggccg attcaccatc tccagagaca acgccaagaa ctcactgtat 240ctgcaaatga acagcctgag agccgaggac acggctgtgt attactgtgc gagagatcaa 300ggtatagcag tggctgggcc ctttgactac tggggccagg gaaccctggt caccgtctcc 360tcagcc 3662122PRTHomo Sapiens 2Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly1 5 10 15Ser Leu Arg Leu Ser Cys Ala Val Ser Gly Phe Thr Phe Ser Ser Tyr 20 25 30Trp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 35 40 45Ala Asn Ile Lys Gln Asp Gly Ser Glu Lys Tyr Tyr Val Asp Ser Val 50 55 60Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr65 70 75 80Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95Ala Arg Asp Gln Gly Ile Ala Val Ala Gly Pro Phe Asp Tyr Trp Gly 100 105 110Gln Gly Thr Leu Val Thr Val Ser ...
ANGPT2 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ANGPT2. (KA1867) - Products - Abnova
Detect and quantitate human angiopoietin 1 (ANGPT1) in buffered solution and cell culture supernatants using a homogeneous LANCE Ultra TR-FRET assay.
Camenisch G et al. (2002) ANGPTL3 stimulates endothelial cell adhesion and migration via integrin alpha vbeta 3 and induces blood vessel formation in vivo.. [^] ...
We read with great interest the recent report by Cho et al in a July issue of Circulation Research.1 However, we were struck by a remarkable feature in a Figure 1B of their article, reproduced here in the accompanying Figure (panel I). In the upper right corner there is the unmistakable resemblance to a human face, albeit with a somewhat skeletal appearance. But what is even more surprising is that a very similar face is clearly visible in the lower right panel of their Figure 3B, reproduced here in panel II, even though this image is representative of a different animal that was studied in a separate experiment. A closer examination reveals that not only is this very same ghostly face seen in the previous image, but that the vasculature architecture in these two figures is also identical, apart from slight horizontal compression. The facial resemblance can be even better appreciated after enlargement of the region within the box (panel III). We would thus submit that there has been an ...
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AGPT2 - Angpt2 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector shRNA available for purchase from OriGene - Your Gene Company.
Teilmann, S. C. and Christensen, S. T. (2005), Localization of the angiopoietin receptors Tie-1 and Tie-2 on the primary cilia in the female reproductive organs. Cell Biology International, 29: 340-346. doi: 10.1016/j.cellbi.2005.03.006 ...
Primary congenital glaucoma (PCG) is a leading cause of blindness in children worldwide and is caused by developmental defects in 2 aqueous humor outflow structures, Schlemms canal (SC) and the trabecular meshwork. We previously identified loss-of-function mutations in the angiopoietin (ANGPT) receptor TEK in families with PCG and showed that ANGPT/TEK signaling is essential for SC development. Here, we describe roles for the major ANGPT ligands in the development of the aqueous outflow pathway. We determined that ANGPT1 is essential for SC development, and that Angpt1-knockout mice form a severely hypomorphic canal with elevated intraocular pressure. By contrast, ANGPT2 was dispensable, although mice deficient in both Angpt1 and Angpt2 completely lacked SC, indicating that ANGPT2 compensates for the loss of ANGPT1. In addition, we identified 3 human subjects with rare ANGPT1 variants within an international cohort of 284 PCG patients. Loss of function in 2 of the 3 patient alleles was observed ...
Primary congenital glaucoma (PCG) is a leading cause of blindness in children worldwide and is caused by developmental defects in 2 aqueous humor outflow structures, Schlemms canal (SC) and the trabecular meshwork. We previously identified loss-of-function mutations in the angiopoietin (ANGPT) receptor TEK in families with PCG and showed that ANGPT/TEK signaling is essential for SC development. Here, we describe roles for the major ANGPT ligands in the development of the aqueous outflow pathway. We determined that ANGPT1 is essential for SC development, and that Angpt1-knockout mice form a severely hypomorphic canal with elevated intraocular pressure. By contrast, ANGPT2 was dispensable, although mice deficient in both Angpt1 and Angpt2 completely lacked SC, indicating that ANGPT2 compensates for the loss of ANGPT1. In addition, we identified 3 human subjects with rare ANGPT1 variants within an international cohort of 284 PCG patients. Loss of function in 2 of the 3 patient alleles was observed ...
The functional consequences of angiopoietin/Tie-2 signaling have been well established through genetic loss-of-function and gain-of-function experiments (Carmeliet, 2003; Maisonpierre et al., 1997; Suri et al., 1996; Yancopoulos et al., 2000). The phenotypes of Ang-1- and Ang-2-deficient and -overexpressing mice have led to an agonistic Ang-1/Tie-2 model and an antagonistic Ang-2/Tie-2 model (Hanahan, 1997). According to these, Ang-1 activates Tie-2 and induces subsequent signal transduction promoting endothelial-cell survival, endothelial quiescence and vessel assembly. Conversely, Ang-2 is believed to act as a non-signal-transducing Tie-2 ligand that binds to endothelial Tie-2 and thereby negatively interferes with agonistic Ang-1/Tie-2 functions. As such, it does not exert functions of its own but rather acts as a facilitator of other vascular cytokines. The net outcome of Ang-2 functions is therefore considered to be contextually determined by the presence of other cytokines. For example, ...
Plasmodium knowlesi parasitemia correlated with age (Spearmans correlation coefficient [rs] = 0.36; P < .0001). In knowlesi malaria, IL-6, angiopoietin-2, and microvascular dysfunction were increased in severe compared to nonsevere disease, and all correlated with age, independent of parasitemia. In falciparum malaria, angiopoietin-2 increased with age, independent of parasite biomass (histidine-rich protein 2 [HRP2]). Independent risk factors for severe malaria included parasitemia and angiopoietin-2 in knowlesi malaria, and HRP2, angiopoietin-2, and microvascular dysfunction in falciparum malaria ...
Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE-1) is a tyrosine kinase cell-surface receptor that is expressed primarily in endothelial cells. It exhibits high homology to TEK/TIE-2 and inhibits the binding of TEK/TIE-2 to the growth factor angiopoietin-1. The extracellular domain of TIE-1 can be cleaved by multiple factors, including vascular endothelial growth factor (VEGF); this results in loss of its ability to inhibit TEK/TIE-2. In addition to its role in angiogenesis, TIE-1 is reported to have proinflammatory effects in endothelial cells. TIE-1 is also known as tyrosine-protein kinase receptor Tie-1, TIE, and JTK14.. ...
The endothelial tyrosine kinase TEK is a cell-surface receptor that binds the growth factor angiopoietin-1. It is closely related to the TIE receptor tyrosine kinase. TEK-mediated signaling regulates angiogenesis, reorganization of the actin cytoskeleton, and survival, proliferation, migration, adhesion, and spreading of endothelial cells. Activation of TEK leads to downstream activation of MAPK1/ERK2 and MAPK3/ERK1 kinases. Mutations in the TEK gene are associated with inherited venous malformations. TEK is also known as TEK tyrosine kinase, endothelial; endothelial tyrosine kinase, Tunica interna endothelial cell kinase, tyrosine kinase with Ig and EGF homology domains-2 (TIE-2), hTIE2, p140 TEK, CD202b, angiopoietin-1 receptor, VMCM, and VMCM1.. ...
TIE-1 and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)- like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Murine TIE-1 cDNA encodes a 1134 amino acid (aa) precursor protein with a 22 aa putative signal peptide, a 733 aa extracellular domain and a 354 aa cytoplasmic domain. Whereas two ligands have been described for TIE-2 [angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2)], so far no ligand was found for TIE-1. Recombinant murine soluble TIE-1 was fused with the Fc part of human IgG1. The recombinant mature sTIE-1/hFc is a disulfide-linked homodimeric protein. The sTIE-1/hFc monomers have a mass ...
Pre-eclampsia is a major cause of maternal and perinatal morbidity and mortality worldwide. A predictor of pre-eclampsia would enable intervention, close surveillance and timely delivery, and thereby reduce the negative consequences of the disorder.. The overall aim of this thesis was to study potential predictors of pre-eclampsia by biochemical and epidemiological methods.. Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) are regulators of angiogenesis, which is important for placental development. In a prospective and longitudinal study of a low-risk population the Ang-1/Ang-2 ratio was evaluated. The Ang-1/Ang-2 ratio increased during pregnancy in all women but at gestational week 25 and 28 the ratios were significantly lower in women who later developed pre-eclampsia. The relevance of Histidine-rich glycoprotein (HRG), a protein with angiogenic properties, was furthermore evaluated. HRG levels decreased in all women, with significantly lower levels at gestational week 10, 25 and 28 in women ...
What do we mean by "cell type?" Two articles in this issue of Circulation Research address this question in different ways. The first study, by Dube et al,1 uses very well-defined tools to define endothelial differentiation at the level of transcriptional control. The second study, by Kowal et al,2 looks for novel genes that distinguish two cell types. These studies, taken together, illustrate a major change in how we define cell type, a change that is about to be accelerated by the power of systematic genomics.. Dube et al1 use in vitro systems to explore the promoter structure for the endothelial cell-specific receptor tyrosine kinase Tie2. Tie2 is a receptor for both angiopoetin-1 and angiopoetin-2. Like vascular endothelial growth factor, angiopoetin-1 is essential for normal vascular development whereas angiopoetin-2 is a naturally occurring antagonist for angiotensin I and Tie2. Thus, regulation of expression of the Tie2 receptor is likely a key issue in the formation of blood vessels.3 4 ...
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We describe the development of the pulmonary vasculature in the mouse from the first morphological sign of lung development (E9.5) until early pseudoglandular stage (E13.5) through the analysis of whole mount X-gal-stained fetal lungs of Tie2-LacZ transgenic mice. The transgenic strain expresses the bacterial lacZ gene under the control of the Tie2 promoter, and cells that convert the X-gal substrate are positive for the angiopoietin receptor Tie2 (31). We expanded our analysis of the pulmonary vasculature using immunohistochemistry on serial sections of wild-type mice with two distinct endothelial-specific cell markers, PECAM-1 and Fli-1. Furthermore, the embryos have been isolated and processed without disrupting the circulation to leave the vascular tone and integrity intact. This procedure prevents the collapse of vessels and the putative creation of artifacts in the sections. Therefore, we were able to follow individual structures throughout serial sections, which allowed us to identify ...
This study identifies a basic biological role for PHD2 in the control of a specific proarteriogenic macrophage phenotype that impinges on TIE2 signalling. Overall, the mechanisms described in this study may represent an indirect modality by which PHD2 modulates oxygen delivery through the regulation of vessel morphogenesis.. The proarteriogenic tissue macrophages identified here are highly reminiscent of the M2-like, proangiogenic TEMs found in tumours, developing organs and regenerating tissues (Capobianco et al, 2011; De Palma et al, 2005; Fantin et al, 2010; Mazzieri et al, 2011; Pucci et al, 2009). Despite the well-characterized proangiogenic functions of TEMs, no studies have yet been undertaken to rigorously assess the functional relevance of the ANG receptor TIE2 in macrophages in the context of ischaemia. We have previously shown that, similar to tumour-associated TEMs (Pucci et al, 2009), Phd2+/− macrophages do not upregulate either Vegf or inflammatory genes, but instead express ...
DCE-MRI Provides Evidence for Vascular Effects of AMG 386, a First-In-Class Anti-Angiogenic Peptibody That Specifically Inhibits Interaction of Angiopoietins-1 and -2 with Tie-2. Yuying C. Hwang1, Ed Ashton2, Lee Rosen3, Roy Herbst4, Jeffrey Silverman5, Ngocdiep Le1, Erik Rasmussen1, Jon Oliner1, Juan Leal6, Robert Radinsky1, Ji-Rong Sun1, Jeff Evelhoch1, Ed Jackson4. 1Amgen Inc., Thousand Oaks, California , USA; 2VirtualScopics Inc., Rochester, New York, USA; 3Premiere Oncology, Santa Monica, California , USA; 4The University of Texas M.D. Anderson Cancer Center, Houston, Texas, USA; 5Landmark Imaging, Los Angeles, California , USA; 6University of Minnesota, Twin Cities, Minnesota, USA. AMG 386 is a peptide-fc fusion protein (peptibody) that specifically inhibits the interaction of angiopoietins-1 and -2 (Ang1/2) with Tie-2 receptor. We performed DCE-MRI in Colo205 xenografts and in the first in human (FIH) clinical trial to study the effect of AMG 386 on tumor vasculature. Both pre-clinical ...
A family of structurally-related angiogenic proteins of approximately 70 kDa in size. They have high specificity for members of the TIE RECEPTOR FAMILY ...
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Angiopoietin-2 (Ang-2) is released from endothelial cells only in response to stimulus (e.g. wound healing, tumor growth) and facilitates blood vessel sprouting and inhibits pericyte-endothelial cell interaction via Tie2 signaling. In tumors, Ang-2 is up-regulated and acts together with the VEGF/VEGFR2 pathway to stimulate tumor angiogenesis and metastasis. While therapeutic intervention using antagonists to the VEGF/VEGFR2 pathway has proven to be successful in limiting disease progression in a number of different clinical settings, there is an obvious need for an improved response. In various preclinical mouse angiogenesis or xenograft models, the combination treatment with anti-Ang2 antibody and the VEGF/VEGFR blocker provided additional benefit over inhibiting the individual pathway. Here as an alternative to combo therapy, we have engineered a tetravalent IgG-scFv bispecific antibody, LY3207447. LY3207447 is an immunoglobulin G4 (IgG4) antibody, comprising of VEGFR2 antibody derived from ...
A single gene called Angiopoietin-1 drives brain size and intelligence in fish according to a new study by researchers at Stockholm University, UCL an
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Pre-eclampsia is a major cause of maternal and perinatal morbidity and mortality worldwide. A predictor of pre-eclampsia would enable intervention, close surveillance and timely delivery, and thereby reduce the negative consequences of the disorder.. The overall aim of this thesis was to study potential predictors of pre-eclampsia by biochemical and epidemiological methods.. Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) are regulators of angiogenesis, which is important for placental development. In a prospective and longitudinal study of a low-risk population the Ang-1/Ang-2 ratio was evaluated. The Ang-1/Ang-2 ratio increased during pregnancy in all women but at gestational week 25 and 28 the ratios were significantly lower in women who later developed pre-eclampsia. The relevance of Histidine-rich glycoprotein (HRG), a protein with angiogenic properties, was furthermore evaluated. HRG levels decreased in all women, with significantly lower levels at gestational week 10, 25 and 28 in women ...
14-3-3 beta, 14-3-3 epsilon, 14-3-3 eta, 14-3-3 gamma, 14-3-3 theta, 14-3-3 zeta, 4E-BP1, 5-HT-1A, 5-HT-1F, 5-HT-2C, 5-HT-3A, 5-HT-4, 5-HT-5A, 60S Ribosomal Protein L10, 6-Phosphofructo-2-Kinase, A1BG, A26C2/3, AARSD1, AASDHPPT, AATF, ABCA8, ABCB7, ABCD1, ABHD11, ABHD12, ABHD12B, ABHD14A, ABHD14B, ABHD4, ABL1, ACAD10, ACBD6, ACOT2, ACOT4, ACSL6, Actin-alpha-1, Actin-gamma2, Actin-pan, ACTL6A, ACTN 1/2/3/4, ACTN alpha-2/3, ACTR-1C, ACTR3, ACVL1, ADA2L, ADAM 17 (Cleaved-Arg215), ADAR1, ADCK1, ADCK2, ADCK3, ADCK5, ADCY4, ADCY5/6, ADCY7, ADCY8, ADD2, ADD3, ADH7, ADK, ADNP, ADPGK, ADRB1, Adrenergic Receptor alpha-2A, Adrenergic Receptor alpha-2B, Adrenergic Receptor alpha-2C, Aggrecan (Cleaved-Asp369), AGR3, AIFM2, AIG1, AIRE, AKR1B1, AKR1CL1, AKR1CL2, AKT, AKT2, AKT3, ALCAM, ALDH1A2, ALDH1B1, ALDH3B1, ALDOB, ALDOC, Alpha Fetoprotein (AFP), Alpha hCG, AMACR, AMPD1, Amylin, Androgen receptor, Angiopoietin-1, Angiopoietin-2, Annexin A6, AOS1, AP-2, AP2C, APAF-1-ALT, APC, APC6, APLP2, APOF, APOL1, ...
TIE2 a tyrosine kinase receptor of the Tie family. Receptor for angiopoietin 1. Expressed almost exclusively in endothelial cells. May constitute the earliest mammalian endothelial cell lineage marker. Appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase. Findings establish that simultaneous Tie 2 activation and Angiopoietin 2 inhibition form a powerful therapeutic strategy to elicit a favorable tumor microenvironment and enhanced delivery of a chemotherapeutic agent into tumors 1). ...
OBJECTIVES: Tie2 and its ligands, the angiopoietins (Ang), are required for embryonic and postnatal angiogenesis. Previous studies have demonstrated that Tie2 is proteolytically cleaved from endothelial cells and produces a 75 kDa soluble receptor fragment (sTie2), however, the signaling mechanisms and effector molecules responsible for the shedding phenomenon are unknown. We investigated mechanisms responsible for Tie2 shedding.. MATERIALS: Human umbilical vein endothelial cells (HUVECs), NIH-3T3, or HEK293 cells exogenously expressing full-length Tie2 were serum starved for varying timepoints. The conditioned media (CM) were then analyzed for the presence of Tie2 by ELISA or westernblot using a Tie2-specific antibody recognizing the extracellular domain. VEGF-A165 (R&D systems) was used for experimentation. Pharmacologic inhibitors of p38 MAPK, Akt, and PI3K were also used. Adenovirus encoding myristylated Akt, wildtype PTEN, dominant negative PTEN and catalytically inactive PTEN were used to ...
La dérégulation de la formation et lintégrité des vaisseaux sanguins peut conduire à un état pathologique tel quobservé dans de nombreuses maladies ischémiques telles que: la croissance de tumeur solide, larthrite rhumatoïde, le psoriasis, les rétinopathies et lathérosclérose. Par conséquent, la possibilité de moduler langiogenèse régionale chez les patients souffrant dischémie est cliniquement pertinente. Un élément clé dans linduction de langiogenèse pathologique est une inflammation qui précède et accompagne la formation des nouveaux vaisseaux. Ce phénomène est démontré par laugmentation de la perméabilité vasculaire et le recrutement de monocytes/ macrophages et cellules polynucléaires (neutrophiles). En collaboration avec dautres groupes, nous avons montré que différents facteurs de croissance tels que le facteur de croissance endothélial vasculaire et les angiopoïétines peuvent non seulement promouvoir langiogenèse mais aussi induire diverses ...
Molecules of the Angiopoietin/Tie ligand/receptor family exert gatekeeper functions within the vascular system to control tissue homeostasis and serve metabolic maintenance functions. Future work of the laboratory will be aimed at better understanding the molecular mechanisms of the vessel wall in the control of tissue homeostasis, most notably as it relates to maintenance and repair processes. Experimental approaches include (i) the vascular control of liver regeneration and liver tumorigenesis, (ii) the role of blood vessels in contributing to fibrotic tissue remodeling, (iii) the role of the vasculature in controlling tissue metabolism, including obesity, (iv) the role of blood vessels during aging and the role of vessel wall resident and bone marrow-derived stem cells during this process, and (v) the role of blood vessels in mediating the metastatic dissemination of circulating tumor cells. The lab will further intensify its efforts to elucidate the molecular and functional properties of the ...
Complete information for ANGPTL2 gene (Protein Coding), Angiopoietin Like 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
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Neuroendocrine tumours (NETs) are uncommon tumours which have a diverse biology. The aims of this thesis were to identify potential new biomarkers and further develop understanding of tumour biology in NETs. The following were assessed i) somatostatin receptor (SSTR) and dopamine-2 receptor (D2R) expression in NETs, ii)HER expression and their associated prognosis, iii) Angiopoietin expression in NETs and their prognostic significance, iv) proteomic analysis of serum and NET cell lines to identify novel markers and finally v) the role of 68Ga-DOTATATE PET in imaging of NETs. Immunohistochemical studies were performed to determine whether SSTR and D2R are co-expressed in NETs. D2R was co-expressed with SSTR-2 and -5 in 93% of low grade tumours, with lower co-expression in higher grade tumours. HER family of receptors are involved in oncogenesis; the expressions of these receptors were assessed. Immunohistochemical analysis of these receptors was performed in 82 cases. EGFR was expressed in 86%, ...
Genomic abnormalities lead to aberrant cell growth and signalling, contributing to the development of malignancy. Growth and metastasis also require the development of tumour vasculature. The VEGFs and angiopoietins are growth factor families central to this process. This thesis examines their expression in endometrial cancer. Genomic and post-genomic studies are used to broaden the investigation of angiogenesis and include related pathways. Using in situ hybridisation VEGF-A mRNA was detected in epithelial cancer cells but not pre-malignant and benign endometrium while VEGF-B was localised to both epithelial and stromal cells and was most abundant in benign endometrium. These findings suggest that VEGF-B may modulate the actions of VEGF-A by occupying receptors. Larger, gene microarray studies confirmed the differential expression of angiogenic factors and also identified a distinct set of coherently regulated genes in endometrial cancers. Within this gene set, PPARα, a transcription factor ...
Venous malformations (VMs) are composed of ectatic veins with scarce smooth muscle cell coverage. Activating mutations in the endothelial cell tyrosine kinase receptor TIE2 are a common cause of these lesions. VMs cause deformity, pain, and local intravascular coagulopathy, and they expand with time. Targeted pharmacological therapies are not available for this condition. Here, we generated a model of VMs by injecting HUVECs expressing the most frequent VM-causing TIE2 mutation, TIE2-L914F, into immune-deficient mice. TIE2-L914F-expressing HUVECs formed VMs with ectatic blood-filled channels that enlarged over time. We tested both rapamycin and a TIE2 tyrosine kinase inhibitor (TIE2-TKI) for their effects on murine VM expansion and for their ability to inhibit mutant TIE2 signaling. Rapamycin prevented VM growth, while TIE2-TKI had no effect. In cultured TIE2-L914F-expressing HUVECs, rapamycin effectively reduced mutant TIE2-induced AKT signaling and, though TIE2-TKI did target the WT receptor, ...
Expression of ANGPT1 (Ang1, KIAA0003) in salivary gland tissue. Antibody staining with HPA018793, HPA018816 and CAB017815 in immunohistochemistry.
package NewScalar; require Tie::Scalar; @ISA = qw(Tie::Scalar); sub FETCH { ... } # Provide a needed method sub TIESCALAR { ... } # Overrides inherited method package NewStdScalar; require Tie::Scalar; @ISA = qw(Tie::StdScalar); # All methods provided by default, so define only what needs be overridden sub FETCH { ... } package main; tie $new_scalar, NewScalar; tie $new_std_scalar, NewStdScalar ...
package NewScalar; require Tie::Scalar; @ISA = qw(Tie::Scalar); sub FETCH { ... } # Provide a needed method sub TIESCALAR { ... } # Overrides inherited method package NewStdScalar; require Tie::Scalar; @ISA = qw(Tie::StdScalar); # All methods provided by default, so define only what needs be overridden sub FETCH { ... } package main; tie $new_scalar, NewScalar; tie $new_std_scalar, NewStdScalar ...
package NewScalar; require Tie::Scalar; @ISA = qw(Tie::Scalar); sub FETCH { ... } # Provide a needed method sub TIESCALAR { ... } # Overrides inherited method package NewStdScalar; require Tie::Scalar; @ISA = qw(Tie::StdScalar); # All methods provided by default, so define # only what needs be overridden sub FETCH { ... } package main; tie $new_scalar, NewScalar; tie $new_std_scalar, NewStdScalar ...
We investigated whether the angiogenic profile, which is based on the local expression and systemic levels of angiogenic growth factors (VEGF, Ang-1, Ang-2, and the corresponding receptors), differs between rheumatoid arthritis (RA) and osteoarthritis (OA) patients. We determined the expression of VEGF, Ang-1, and Ang-2 together with its receptors (VEGFR-1/-2 ...
Rat monoclonal Angiopoietin 3 antibody [RM0073-2H34] validated for WB and tested in Mouse. Immunogen corresponding to recombinant full length protein
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Angiopoietins (Ang) are vascular endothelial cell-specific growth factors that play important roles principally during the later stages of angiogenesis. We have compared the distribution of the...
Principal Investigator:KATO Tetsuro, Project Period (FY):1998 - 2000, Research Category:Grant-in-Aid for Scientific Research (B)., Section:一般, Research Field:Urology
Our results show that genetic elimination of BAMBI as a modulator of the signaling of the family of TGF cytokines renders the almost-resistant C57BL/6 mouse strain more susceptible to the development of diabetic glomerular abnormalities, as determined by proteinuria with a widening of the FPs. This is associated with increased activation of ERK1/2 and Smad1/5 alternative TGF-β signaling pathways. The Vegfr2 and Angpt1 genes, which control glomerular endothelial survival and microvascular stability, were downmodulated in glomeruli from diabetic BAMBI−/− mice compared with BAMBI+/+ mice. Incubation of glomeruli from nondiabetic BAMBI+/+ or BAMBI−/− mice with TGF-β resulted in further downregulation of Angpt1 and Vegfr2 in glomeruli from BAMBI−/− compared with BAMBI+/+ mice. The enhanced downregulation of Vegfr2 in glomeruli of diabetic mice by eliminating BAMBI could be localized to glomerular endothelial cells using an H2B-EYFP reporter under the control of Vegfr2/Flk1 regulatory ...
J:165754 Watanabe T, Koibuchi N, Chin MT, Transcription factor CHF1/Hey2 regulates coronary vascular maturation. Mech Dev. 2010 Sep-Dec;127(9-12):418-27 ...
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Browse stories and reviews on Anobii of Everything Is Illuminated tie-in written by Jonathan Safran Foer, published by Harper Perennial in format Paperback
EK1711大鼠角化细胞内分泌因子(KAF)/双调蛋白(AR)ELISA试剂盒Ratkeratinocyteautocrinefactor/Amphiregulin,KAF/ARELISAkitEK1712大鼠白细胞活化黏附因子(ALCAM)ELISA试剂盒RatActivatedLeukocyteCellAdhesionMolecule,ALCAMELISAkitEK1713大鼠白介素9(IL-
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Vessel maturation involves recruitment of mural cells. Laminar shear stress is a major trigger for vessel maturation. However, the molecular mechanisms by which shear stress affects recruitment of pericytes are unclear. MicroRNAs are small non-coding RNAs, which post-transcriptionally control gene expression. Since shear stress regulates various miRs, we hypothesize that flow-induced miRs inhibit repulsive cues and facilitate mural cell coverage.. Laminar shear stress for 72h induces the up-regulation of miR-27b (2.8±0.24-fold vs static, p,0.05) in cultured endothelial cells (ECs) and in mouse femoral artery segments that were exposed to physiological shear stress ex vivo (1.5±0.14-fold vs no flow, p,0.05). Predicted targets for miR-27b include members of the semaphorin (SEMA) family (known to regulate repulsive signaling) and angiopoietin-2 (Ang2), which causes vessel destabilization. MiR-27b overexpression reduces SEMA6A (63.5±13.5%), SEMA6D (58±26%), and Ang2 (51.5±11%) ...
Meyer Nuala J, Li Mingyao, Feng Rui, Bradfield Jonathan, Gallop Robert, Bellamy Scarlett, Fuchs Barry D, Lanken Paul N, Albelda Steven M, Rushefski Melanie, Aplenc Richard, Abramova Helen, Atochina-Vasserman Elena N, Beers Michael F, Calfee Carolyn S, Cohen Mitchell J, Pittet Jean-Francois, Christiani David C, OKeefe Grant E, Ware Lorraine B, May Addison K, Wurfel Mark M, Hakonarson Hakon, Christie Jason D: ANGPT2 Genetic Variant Is Associated with Trauma-associated Acute Lung Injury and Altered Plasma Angiopoietin-2 Isoform Ratio. American Journal of Respiratory and Critical Care Medicine 183(10): 1344-53, May 2011 ...
Background. Peritubular capillary injury induces chronic hypoxia in the renal tubulointerstitium, and renal peritubular capillary dysfunction is an early event that contributes to tubulointerstitial fibrosis. Cyclosporine A (CsA) is a potent immunosuppressant and improves survival of renal allografts. However, the limitation of CsA use is chronic nephrotoxicity. A soluble, stable and potent angiopoietin-1 (Ang1) variant, cartilage oligomeric matrix protein (COMP)-Ang1 has been developed. We investigated whether COMP-Ang1 ameliorates CsA-induced renal injury.. Methods. CsA-treated mice were injected with recombinant adenovirus expressing either COMP-Ang1 or LacZ. Histology, inflammatory, haemodynamic and fibrotic parameters, and signalling pathway were evaluated.. Results. Histologic examination showed that COMP-Ang1 significantly decreased CsA-induced tubular damage and tubulointerstitial fibrosis. CsA-induced increases in macrophage infiltration and expression of MCP-1 and ICAM-1 after CsA ...

Plasma angiopoietin-1, angiopoietin-2, and angiopoietin receptor tie-2 levels in congestive heart failure | JACC: Journal of...Plasma angiopoietin-1, angiopoietin-2, and angiopoietin receptor tie-2 levels in congestive heart failure | JACC: Journal of...

angiopoietin. CHF. congestive heart failure. CV. coefficient of variation. ELISA. enzyme-linked immunosorbent assay. HMG-CoA. ... angiopoietin-2, and angiopoietin receptor tie-2 levels in congestive heart failure ... angiopoietin-2, and angiopoietin receptor tie-2 levels in congestive heart failure ... angiopoietin-2, and angiopoietin receptor tie-2 levels in congestive heart failure ...
more infohttp://www.onlinejacc.org/content/43/3/423

Angiopoietin-like Protein 1/ANGPTL1 Proteins: Novus BiologicalsAngiopoietin-like Protein 1/ANGPTL1 Proteins: Novus Biologicals

Browse our Angiopoietin-like Protein 1/ANGPTL1 Protein catalog backed by our Guarantee+. ... Angiopoietin-like Protein 1/ANGPTL1 Proteins available through Novus Biologicals. ... angiopoietin Y1 protein, angiopoietin-3 protein, angiopoietin-like 1 protein, Angiopoietin-like protein 1 protein, angiopoietin ... Our Angiopoietin-like Protein 1/ANGPTL1 Peptides and Angiopoietin-like Protein 1/ANGPTL1 Proteins can be used in a variety of ...
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Corrections: Hypoxia-inducible factor 1 alpha-activated angiopoietin-like protein 4 contributes to tumor metastasis via...Corrections: Hypoxia-inducible factor 1 alpha-activated angiopoietin-like protein 4 contributes to tumor metastasis via...

1. Dongjun Luo, Zhongxia Wang, Junyi Wu, Chunping Jiang, Junhua Wu, The Role of Hypoxia Inducible Factor-1 in Hepatocellular ... Hypoxia-inducible factor 1 alpha-activated angiopoietin-like protein 4 contributes to tumor metastasis via vascular cell ... Corrections: Hypoxia-inducible factor 1 alpha-activated angiopoietin-like protein 4 contributes to tumor metastasis via ... adhesion molecule-1/integrin β1 signaling in human hepatocellular carcinoma Volume 54, Issue 3, 910-919, Article first ...
more infohttp://onlinelibrary.wiley.com/doi/10.1002/hep.24669/abstract?globalMessage=0

Inactivation of lipoprotein lipase occurs on the surface of THP-1 macrophages where oligomers of angiopoietin-like protein 4...Inactivation of lipoprotein lipase occurs on the surface of THP-1 macrophages where oligomers of angiopoietin-like protein 4...

Angiopoietin-like protein 4, Lipoprotein lipase, Macrophages, GW501516 National Category Biochemistry and Molecular Biology ... Angiopoietin-like protein (ANGPTL) 4 inactivates LPL and ANGPTL4 expression is controlled by peroxisome proliferator-activated ... We conclude that inactivation of LPL in THP-1 macrophages primarily occurs on the cell surface where oligomers of ANGPTL4 are ... Inactivation of lipoprotein lipase occurs on the surface of THP-1 macrophages where oligomers of angiopoietin-like protein 4 ...
more infohttp://umu.diva-portal.org/smash/record.jsf?pid=diva2:559139

Response of angiopoietin-like proteins 3 and 4 to hemodialysis. | Sigma-AldrichResponse of angiopoietin-like proteins 3 and 4 to hemodialysis. | Sigma-Aldrich

Response of angiopoietin-like proteins 3 and 4 to hemodialysis.. [Dana Mahmood, Elena Makoveichuk, Solveig Nilsson, Gunilla ... Patients on chronic hemodialysis (cHD) have decreased activity of lipoprotein lipase (LPL). Angiopoietin-like proteins (ANGPTL ... o-Phenylenediamine dihydrochloride, tablet, 1 mg substrate per tablet C6H8N2 · 2HCl ...
more infohttps://www.sigmaaldrich.com/catalog/papers/24634330

A Polyglucuronic Acid to Target the FIAF Adipokine for Slimming EffectsA Polyglucuronic Acid to Target the FIAF Adipokine for Slimming Effects

... angiopoietin-related protein 4 (ARP4), PPARγ angiopoietin related gene (PGAR), ANGPTL2, pp1158 and NL2. ... 6. S Mandard, F Zandbergen, E Va Straten, W Wahli, M Muller and S Kersten, The fasting induced adipose factor/angiopoietin-like ... It belongs to the family of fibrinogen/angiopoietinlike proteins and is also referred to as: angiopoietin-like protein 4 ( ... 5. A Xu et al, Angiopoietin-like protein 4 decreases blood glucose and improves glucose tolerance but induces hyperlipidemia ...
more infohttp://www.cosmeticsandtoiletries.com/formulating/function/active/premium-A-Polyglucuronic-Acid-to-Target-the-FIAF-Adipokine-for-Slimming-Effects-211022481.html

Angiopoietin 1 - WikipediaAngiopoietin 1 - Wikipedia

... increased angiopoietin-2, and an elevated ratio of angiopoietin-2/angiopoietin-1 in the placenta. This suggests that ... Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar ... may be associated with a dysregulation in angiopoietins. Increased levels of angiopoietin-1 appear to be associated with a ... Angiopoietin 1 has been shown to interact with TEK tyrosine kinase. Recently, studies in malaria-endemic areas suggest that ...
more infohttps://en.wikipedia.org/wiki/Angiopoietin_1

Angiopoietin-1 for Myocardial Angiogenesis | IntechOpenAngiopoietin-1 for Myocardial Angiogenesis | IntechOpen

Angiopoietin-1 for Myocardial Angiogenesis , IntechOpen, Published on: 2011-10-21. Authors: Vien Khach Lai, Muhammad Zeeshan ... Angiopoietin-1 for Myocardial Angiogenesis. By Vien Khach Lai, Muhammad Zeeshan Afzal, Muhammad Ashraf and Khawaja Husnain ...
more infohttps://www.intechopen.com/books/myocarditis/angiopoietin-1-for-myocardial-angiogenesis/

Recombinant human Angiopoietin 1 protein (ab69492)Recombinant human Angiopoietin 1 protein (ab69492)

Buy our Recombinant human Angiopoietin 1 protein. Ab69492 is an active full length protein produced in HeLa cells and has been ... Recombinant human Angiopoietin 1 protein. See all Angiopoietin 1 proteins and peptides. ...
more infohttp://www.abcam.com/recombinant-human-angiopoietin-1-protein-ab69492.html

Angiopoietin-1 Uncouples the VEGF Receptor from Src | Science SignalingAngiopoietin-1 Uncouples the VEGF Receptor from Src | Science Signaling

Angiopoietin-1 Uncouples the VEGF Receptor from Src Message Subject. (Your Name) has forwarded a page to you from Science ... J. S. Gutkind, Angiopoietin-1 prevents VEGF-induced endothelial permeability by sequestering Src through mDia. Dev. Cell 14, 25 ... Angiopoietin-1 (Ang1) and vascular endothelial growth factor (VEGF) play complementary roles in vascular development during ... extracellular signal-regulated kinases 1 and 2, and focal adhesion kinase) but did prevent disruption of endothelial barrier ...
more infohttp://stke.sciencemag.org/content/1/3/ec24

JCI -
Angiopoietin-1 is required for Schlemms canal development in mice and humansJCI - Angiopoietin-1 is required for Schlemm's canal development in mice and humans

We previously identified loss-of-function mutations in the angiopoietin (ANGPT) receptor TEK in families with PCG and showed ... Angiopoietin-1 is required for Schlemms canal development in mice and humans. ... Angiopoietin-1 is required for Schlemms canal development in mice and humans. ... Published December 1, 2017; First published November 6, 2017 Citation Information: J Clin Invest. 2017;127(12):4421-4436. https ...
more infohttps://www.jci.org/articles/view/95545/pdf

Correction: Article on Role of Angiopoietin-1 in Tumor Metastasis | Cancer ResearchCorrection: Article on Role of Angiopoietin-1 in Tumor Metastasis | Cancer Research

Holopainen T, Huang H, Chen C, Kim KE, Zhang L, Zhou F, Han W, Li C, Yu J, Wu J, Koh GY, Alitalo K, He Y. Angiopoietin-1 ... Correction: Article on Role of Angiopoietin-1 in Tumor Metastasis Message Subject (Your Name) has forwarded a page to you from ... In the article on the role of angiopoietin-1 in tumor metastasis in the June 1, 2009 issue of Cancer Research ( 1), the authors ... Tanja Holopainen,2 Huilian Huang,1 Caiping Chen,1 Kyung Eun Kim,3 Luqing Zhang,1 Fei Zhou,1 Wencan Han,1 Chaojun Li,1 Jun Yu,4 ...
more infohttp://cancerres.aacrjournals.org/content/69/13/5618.2

Pericyte-Derived Dickkopf2 Regenerates Damaged Penile Neurovasculature Through an Angiopoietin-1-Tie2 Pathway | DiabetesPericyte-Derived Dickkopf2 Regenerates Damaged Penile Neurovasculature Through an Angiopoietin-1-Tie2 Pathway | Diabetes

Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning. Cell 1996;87:1161-1169pmid: ... Angiopoietin-1 promotes neurite outgrowth from dorsal root ganglion cells positive for Tie-2 receptor. Cell Tissue Res 2005;320 ... Designed angiopoietin-1 variant, COMP-angiopoietin-1, rescues erectile function through healthy cavernous angiogenesis in a ... Effect of intracavernous administration of angiopoietin-4 on erectile function in the streptozotocin-induced diabetic mouse. J ...
more infohttps://diabetes.diabetesjournals.org/content/67/6/1149

Requisite role of angiopoietin-1, a ligand for the TIE2 receptor, during embryonic angiogenesis.  - PubMed - NCBIRequisite role of angiopoietin-1, a ligand for the TIE2 receptor, during embryonic angiogenesis. - PubMed - NCBI

Requisite role of angiopoietin-1, a ligand for the TIE2 receptor, during embryonic angiogenesis.. Suri C1, Jones PF, Patan S, ... Angiopoietin-1 seems to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix ... We have recently cloned a ligand for TIE2, termed Angiopoietin-1. Here we show that mice engineered to lack Angiopoietin-1 ... demonstrating that Angiopoietin-1 is a primary physiologic ligand for TIE2 and that it has critical in vivo angiogenic actions ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/8980224?dopt=Abstract

Angiopoietin-1 Promotes Skeletal and Cardiac Myocyte Survival Through Integrins | Circulation ResearchAngiopoietin-1 Promotes Skeletal and Cardiac Myocyte Survival Through Integrins | Circulation Research

Angiopoietin-1 Promotes Skeletal and Cardiac Myocyte Survival Through Integrins. Susan M. Dallabrida, Nesreen Ismail, Julianne ... Angiopoietin-1 Promotes Skeletal and Cardiac Myocyte Survival Through Integrins. Susan M. Dallabrida, Nesreen Ismail, Julianne ... Angiopoietin-1 Promotes Skeletal and Cardiac Myocyte Survival Through Integrins. Susan M. Dallabrida, Nesreen Ismail, Julianne ...
more infohttp://circres.ahajournals.org/content/early/2005/02/03/01.RES.0000158285.57191.60

Angiopoietin-1 Production in Islets Improves Islet Engraftment and Protects Islets From Cytokine-Induced Apoptosis | DiabetesAngiopoietin-1 Production in Islets Improves Islet Engraftment and Protects Islets From Cytokine-Induced Apoptosis | Diabetes

Kwak HJ, So JN, Lee SJ, Kim I, Koh GY: Angiopoietin-1 is an apoptosis survival factor for endothelial cells. FEBS Lett448 :249 ... Daly C, Wong V, Burova E, Wei Y, Zabski S, Griffiths J, Lai KM, Lin HC, Ioffe E, Yancopoulos GD, Rudge JS: Angiopoietin-1 ... Cho CH, Sung HK, Kim KT, Cheon HG, Oh GT, Hong HJ, Yoo OJ, Koh GY: COMP-angiopoietin-1 promotes wound healing through enhanced ... Brindle NP, Saharinen P, Alitalo K: Signaling and functions of angiopoietin-1 in vascular protection. Circ Res98 :1014 -1023, ...
more infohttp://diabetes.diabetesjournals.org/content/56/9/2274

Angiopoietin 1 Polyclonal Antibody by Bioss, Cat. No. bs-0800R | Lucerna-Chem AGAngiopoietin 1 Polyclonal Antibody by Bioss, Cat. No. bs-0800R | Lucerna-Chem AG

Angiopoietin 1 Polyclonal Antibody. Catalog Number. Pack Size. List Price*. Promo Price*. Quantity. ... Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.. ... Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.. ...
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Anti-Mouse (Murine) Angiopoietin 1 antibody for Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))Anti-Mouse (Murine) Angiopoietin 1 antibody for Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Angiopoietin 1 antibody for Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)). ... Recommended Angiopoietin 1 Antibody (supplied by: Log in to see ) Antigen Angiopoietin 1 (ANGPT1) Antibodies show synonyms for ... This Angiopoietin 1 antibody is un-conjugated Alternatives Biotin. (19), FITC. (16), HRP. (8), APC. (4), PE. (4), Alkaline ... Target Details Angiopoietin 1 Product Details anti-Angiopoietin 1 Antibody Application Details Handling Images back to top ...
more infohttp://www.antibodies-online.com/rtk-signaling-pathway-8/angiopoietin-1-antibody-2098/mouse-ihc-p-9205/

Anti-ANGPT1 / Angiopoietin-1 Antibody | Rabbit anti-Mouse Cy3 | LSBioAnti-ANGPT1 / Angiopoietin-1 Antibody | Rabbit anti-Mouse Cy3 | LSBio

Angiopoietin-1 antibody LS-C696965 is a Cy3-conjugated rabbit polyclonal antibody to mouse Angiopoietin-1 (ANGPT1). Validated ... ANGPT1 / Angiopoietin1 Antibody (aa360‑409) LS‑C151507 Species: Mouse, Human, Monkey, Rat, Bat, Bovine, Dog, Horse, Pig, ... Angiopoietin-1 antibody LS-C696965 is a Cy3-conjugated rabbit polyclonal antibody to mouse Angiopoietin-1 (ANGPT1). Validated ... Angiopoietin-1 antibody LS-C696965 is a Cy3-conjugated rabbit polyclonal antibody to mouse Angiopoietin-1 (ANGPT1). Validated ...
more infohttps://www.lsbio.com/antibodies/angpt1-antibody-angiopoietin-1-antibody-aa253-429-cy3-ihc-wb-western-ls-c696965/722393

Mouse Angiopoietin-1 ELISA Kit - Innovative ResearchMouse Angiopoietin-1 ELISA Kit - Innovative Research

Our Mouse Angiopoietin-1 ELISA Kit is a sandwich ELISA intended for the quantitative determination of mouse Angiopoietin-1 in ... Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar ... Mouse Angiopoietin-1 ELISA Kit - 1 Kit (96 Wells). Our Mouse Angiopoietin-1 ELISA Kit is a sandwich ELISA intended for the ... Angiopoietin 1, also called ANG1 is a type of angiopoietin and is encoded by the gene ANGPT1. ...
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Ratio of angiopoietin-2 to angiopoietin-1 as a predictor of mortality in acute lung injury patients.Ratio of angiopoietin-2 to angiopoietin-1 as a predictor of mortality in acute lung injury patients.

We also tested the association of concentration of angiopoietin-2 relative to ang ... To test the hypothesis that the concentration of angiopoietin-2 relative to angiopoietin-1 may be a useful biological marker of ... Baseline concentration of angiopoietin-2 relative to angiopoietin-1 was significantly higher in patients who died (median, 58 [ ... OBJECTIVE: To test the hypothesis that the concentration of angiopoietin-2 relative to angiopoietin-1 may be a useful ...
more infohttp://www.biomedsearch.com/nih/Ratio-angiopoietin-2-to-1/20581666.html

Recombinant Human Angiopoietin 1, FLAG-tagged ANGPT1-434H - Creative BioMartRecombinant Human Angiopoietin 1, FLAG-tagged ANGPT1-434H - Creative BioMart

Recombinant human angiopoietin 1 was produced inCHO cells.Full amino acids (20-498) of Ang1 except the secretory signal ... sequences (amino acid 1-19) for secretion is expressed. A FLAG-tag is fused to N-terminal portion of recombinant Ang1. The ... Recombinant Human Angiopoietin 1, FLAG-tagged. Download Datasheet See All ANGPT1 Products. Bring this labeled protein directly ... Recombinant human angiopoietin 1 was produced inCHO cells.Full amino acids (20-498) of Ang1 except the secretory signal ...
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Hematopoietic stem and progenitor cells regulate the regeneration of their niche by secreting Angiopoietin-1 | eLifeHematopoietic stem and progenitor cells regulate the regeneration of their niche by secreting Angiopoietin-1 | eLife

Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning * S Davis ... Here, we systematically assessed the expression and function of Angiopoietin-1 (Angpt1) in bone marrow. Angpt1 was not ... Tie2/angiopoietin-1 signaling regulates hematopoietic stem cell quiescence in the bone marrow niche * F Arai ... Angiopoietin-1 is essential in mouse vasculature during development and in response to injury * M Jeansson ...
more infohttps://elifesciences.org/articles/05521

Anti-ANGPT1 / Angiopoietin-1 Antibody (clone 171606) for IHC, WB/Western, ELISA LS-C149882Anti-ANGPT1 / Angiopoietin-1 Antibody (clone 171606) for IHC, WB/Western, ELISA LS-C149882

... that binds human angiopoietin-1 (also known as ANGPT1). Validated for ELISA, IHC, and western blot. ... Angiopoietin-1 antibody LS-C149882 is a protein G-purified mouse monoclonal (clone 171606, isotype IgG2b) ... Anti-ANGPT1 / Angiopoietin-1 Antibody (N-Terminus) IHC-plus™ LS-B62 Antibody:. Rabbit Polyclonal to Mouse ANGPT1 / Angiopoietin ... Anti-ANGPT1 / Angiopoietin-1 Antibody (aa76-85) IHC-plus™ LS-B6867 Antibody:. Goat Polyclonal to Human ANGPT1 / Angiopoietin-1 ...
more infohttps://www.lsbio.com/antibodies/anti-angpt1-antibody-angiopoietin-1-antibody-clone-171606-elisa-ihc-wb-western-ls-c149882/155825

Hyperoxia causes miR-34a-mediated injury via angiopoietin-1 in neonata by Mansoor Syed, Pragnya Das et al."Hyperoxia causes miR-34a-mediated injury via angiopoietin-1 in neonata" by Mansoor Syed, Pragnya Das et al.

Administration of angiopoietin-1, which is one of the downstream targets of miR34a, is able to ameliorate the BPD pulmonary and ... Administration of angiopoietin-1, which is one of the downstream targets of miR34a, is able to ameliorate the BPD pulmonary and ... Hyperoxia causes miR-34a-mediated injury via angiopoietin-1 in neonatal lungs. ... "Hyperoxia causes miR-34a-mediated injury via angiopoietin-1 in neonatal lungs." (2017). Department of Pediatrics Faculty Papers ...
more infohttp://jdc.jefferson.edu/pedsfp/73/
  • In a murine mammary carcinoma model, Ang-1 is secreted and then bound to the extracellular matrix (7) where it is ideally situated to mediate cell-to-cell and cell-to-matrix interaction of capillaries (8,9) . (onlinejacc.org)
  • Depletion of resident peritoneal macrophages prior to, or concomitant injections of an anti-CCL4 antibody with MAT.Ang-1 resulted in a significant reduction in neutrophil recruitment. (whiterose.ac.uk)
  • To probe the mechanism of islet revascularization, we determined the effect of angiopoietin-1 (Ang-1), a proangiogenic and antiapoptotic factor, on the survival, function, and revascularization of transplanted islets using a syngeneic model. (diabetesjournals.org)
  • We used a mouse model of peritoneal neutrophilic inflammation to determine if Ang-1 could stimulate neutrophil migration in vivo. (whiterose.ac.uk)
  • Intraperitoneal injection of an engineered angiopoietin-1, MAT.Ang-1, induced significant neutrophil migration into the peritoneum and a significant increase in the levels of CCL4 in peritoneal lavage fluid. (whiterose.ac.uk)
  • Phosphodiesterase type 5 inhibitors enhance the nitric oxide (NO)-cyclic guanosine monophosphate pathway and are currently used as a first-line therapy for ED ( 1 ). (diabetesjournals.org)
  • Ratio of angiopoietin-2 to angiopoietin-1 as a predictor of mortality in acute lung injury patients. (biomedsearch.com)
  • CONCLUSIONS: The ratio of concentration of angiopoietin-2 relative to angiopoietin-1 may be a prognostic biomarker of endothelial activation in acute lung injury patients and, along with pulmonary dead-space fraction, may be useful for risk stratification of acute lung injury patients, particularly in identifying subgroups for future research and therapeutic trials. (biomedsearch.com)
  • Diabetic mice receiving a marginal mass of 150 islets pretransduced with Ang-1 vector exhibited near normoglycemia posttransplantation. (diabetesjournals.org)
  • Islets were transduced with adenoviruses expressing Ang-1 or control LacZ, followed by transplantation under the renal capsule. (diabetesjournals.org)
  • Increased levels of angiopoietin-1 appear to be associated with a decrease in placental weight and placental barrier thickness in women infected with Plasmodium (the causative agent of malaria). (wikipedia.org)
  • 0.001), but there were no significant differences in Ang-1 levels between the groups. (onlinejacc.org)
  • Conclusions We have demonstrated abnormal levels of Ang-2 and tie-2, but normal Ang-1, in both CHF patients. (onlinejacc.org)
  • This study was performed to evaluate the profile of angiopoietin-1, angiopoietin-2, matrix metalloproteinase -2 and -9 and transforming growth factor-β in vitreous of diabetic patients with proliferative and non-proliferative diabetic retinopathy comparing data with controls operated due to nonvascular vitreoretinal diseases. (arvojournals.org)