A genus of gram-negative bacteria whose organisms are obligate parasites of vertebrates. Species are transmitted by arthropod vectors with the host range limited to ruminants. Anaplasma marginale is the most pathogenic species and is the causative agent of severe bovine anaplasmosis.
A species of gram-negative bacteria in the genus ANAPLASMA, family ANAPLASMATACEAE, formerly called Ehrlichia phagocytophila or Ehrlichia equi. This organism is tick-borne (IXODES) and causes disease in horses and sheep. In humans, it causes human granulocytic EHRLICHIOSIS.
A species of gram-negative bacteria and causative agent of severe bovine ANAPLASMOSIS. It is the most pathogenic of the ANAPLASMA species.
A disease of cattle caused by parasitization of the red blood cells by bacteria of the genus ANAPLASMA.
A tick-borne disease characterized by FEVER; HEADACHE; myalgias; ANOREXIA; and occasionally RASH. It is caused by several bacterial species and can produce disease in DOGS; CATTLE; SHEEP; GOATS; HORSES; and humans. The primary species causing human disease are EHRLICHIA CHAFFEENSIS; ANAPLASMA PHAGOCYTOPHILUM; and Ehrlichia ewingii.
A species of gram-negative bacteria causing mild ANAPLASMOSIS in CATTLE. It also can infect SHEEP and GOATS. It is transmitted by TICKS.
A species of gram-negative bacteria producing mild to severe ANAPLASMOSIS in SHEEP and GOATS, and mild or inapparent infections in DEER and CATTLE.
The largest genus of TICKS in the family IXODIDAE, containing over 200 species. Many infest humans and other mammals and several are vectors of diseases such as LYME DISEASE, tick-borne encephalitis (ENCEPHALITIS, TICK-BORNE), and KYASANUR FOREST DISEASE.
Blood-sucking acarid parasites of the order Ixodida comprising two families: the softbacked ticks (ARGASIDAE) and hardbacked ticks (IXODIDAE). Ticks are larger than their relatives, the MITES. They penetrate the skin of their host by means of highly specialized, hooked mouth parts and feed on its blood. Ticks attack all groups of terrestrial vertebrates. In humans they are responsible for many TICK-BORNE DISEASES, including the transmission of ROCKY MOUNTAIN SPOTTED FEVER; TULAREMIA; BABESIOSIS; AFRICAN SWINE FEVER; and RELAPSING FEVER. (From Barnes, Invertebrate Zoology, 5th ed, pp543-44)
A widely distributed genus of TICKS, in the family IXODIDAE, including a number that infest humans and other mammals. Several are vectors of diseases such as TULAREMIA; ROCKY MOUNTAIN SPOTTED FEVER; COLORADO TICK FEVER; and ANAPLASMOSIS.
Bacterial, viral, or parasitic diseases transmitted to humans and animals by the bite of infected ticks. The families Ixodidae and Argasidae contain many bloodsucking species that are important pests of man and domestic birds and mammals and probably exceed all other arthropods in the number and variety of disease agents they transmit. Many of the tick-borne diseases are zoonotic.
Infections with bacteria of the family ANAPLASMATACEAE.
Infestations with soft-bodied (Argasidae) or hard-bodied (Ixodidae) ticks.
Members of the class Arachnida, especially SPIDERS; SCORPIONS; MITES; and TICKS; which transmit infective organisms from one host to another or from an inanimate reservoir to an animate host.
A family of bacteria which inhabit RED BLOOD CELLS and cause several animal diseases.
Small, often pleomorphic, coccoid to ellipsoidal organisms occurring intracytoplasmically in circulating LYMPHOCYTES. They are the etiologic agents of tick-borne diseases of humans; DOGS; CATTLE; SHEEP; GOATS; and HORSES.
A species of protozoa infecting humans via the intermediate tick vector IXODES scapularis. The other hosts are the mouse PEROMYSCUS leucopus and meadow vole MICROTUS pennsylvanicus, which are fed on by the tick. Other primates can be experimentally infected with Babesia microti.
Diseases of domestic cattle of the genus Bos. It includes diseases of cows, yaks, and zebus.
Proteins isolated from the outer membrane of Gram-negative bacteria.
The family Cervidae of 17 genera and 45 species occurring nearly throughout North America, South America, and Eurasia, on most associated continental islands, and in northern Africa. Wild populations of deer have been established through introduction by people in Cuba, New Guinea, Australia, New Zealand, and other places where the family does not naturally occur. They are slim, long-legged and best characterized by the presence of antlers. Their habitat is forests, swamps, brush country, deserts, and arctic tundra. They are usually good swimmers; some migrate seasonally. (Walker's Mammals of the World, 5th ed, p1362)
Arthropods, other than insects and arachnids, which transmit infective organisms from one host to another or from an inanimate reservoir to an animate host.
A genus of tick-borne protozoan parasites that infests the red blood cells of mammals, including humans. There are many recognized species, and the distribution is world-wide.
The immature stage in the life cycle of those orders of insects characterized by gradual metamorphosis, in which the young resemble the imago in general form of body, including compound eyes and external wings; also the 8-legged stage of mites and ticks that follows the first moult.
A species of gram-negative bacteria that is the causative agent of human EHRLICHIOSIS. This organism was first discovered at Fort Chaffee, Arkansas, when blood samples from suspected human ehrlichiosis patients were studied.
A group of tick-borne diseases of mammals including ZOONOSES in humans. They are caused by protozoa of the genus BABESIA, which parasitize erythrocytes, producing hemolysis. In the U.S., the organism's natural host is mice and transmission is by the deer tick IXODES SCAPULARIS.
Animate or inanimate sources which normally harbor disease-causing organisms and thus serve as potential sources of disease outbreaks. Reservoirs are distinguished from vectors (DISEASE VECTORS) and carriers, which are agents of disease transmission rather than continuing sources of potential disease outbreaks.
A promyelocytic cell line derived from a patient with ACUTE PROMYELOCYTIC LEUKEMIA. HL-60 cells lack specific markers for LYMPHOID CELLS but express surface receptors for FC FRAGMENTS and COMPLEMENT SYSTEM PROTEINS. They also exhibit phagocytic activity and responsiveness to chemotactic stimuli. (From Hay et al., American Type Culture Collection, 7th ed, pp127-8)
Diseases of the domestic dog (Canis familiaris). This term does not include diseases of wild dogs, WOLVES; FOXES; and other Canidae for which the heading CARNIVORA is used.
A specific species of bacteria, part of the BORRELIA BURGDORFERI GROUP, whose common name is Lyme disease spirochete.
Genus of gram-negative, intracytoplasmic bacteria that are found primarily in VACUOLES of MONOCYTES in the BLOOD and MACROPHAGES of lymphoid or other tissues of DOGS; HORSES, and humans. (From Int. J. Syst. Evol. Microbiol. (2001) 51:2145-2165)
A family of hardbacked TICKS, in the subclass ACARI. Genera include DERMACENTOR and IXODES among others.
Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
A species of gram-negative bacteria in the family ANAPLASMATACEAE, that causes HEARTWATER DISEASE in ruminants.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Substances elaborated by bacteria that have antigenic activity.
Species of gram-negative bacteria in the family ANAPLASMATACEAE, causing EHRLICHIOSIS in DOGS. The most common vector is the brown dog tick. It can also cause disease in humans.
Diseases of domestic and mountain sheep of the genus Ovis.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Diseases of the domestic or wild goat of the genus Capra.
A genus of TICKS, in the family IXODIDAE, widespread in Africa. Members of the genus include many important vectors of animal and human pathogens.
Glands that secrete SALIVA in the MOUTH. There are three pairs of salivary glands (PAROTID GLAND; SUBLINGUAL GLAND; SUBMANDIBULAR GLAND).
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
A genus of gram-negative, aerobic, rod-shaped bacteria often surrounded by a protein microcapsular layer and slime layer. The natural cycle of its organisms generally involves a vertebrate and an invertebrate host. Species of the genus are the etiological agents of human diseases, such as typhus.
The relationships of groups of organisms as reflected by their genetic makeup.
EPIDEMIOLOGIC STUDIES based on the detection through serological testing of characteristic change in the serum level of specific ANTIBODIES. Latent subclinical infections and carrier states can thus be detected in addition to clinically overt cases.
Change in the surface ANTIGEN of a microorganism. There are two different types. One is a phenomenon, especially associated with INFLUENZA VIRUSES, where they undergo spontaneous variation both as slow antigenic drift and sudden emergence of new strains (antigenic shift). The second type is when certain PARASITES, especially trypanosomes, PLASMODIUM, and BORRELIA, survive the immune response of the host by changing the surface coat (antigen switching). (From Herbert et al., The Dictionary of Immunology, 4th ed)
An island of the West Indies. Its capital is St. George's. It was discovered in 1498 by Columbus who called it Concepcion. It was held at different times by the French and the British during the 18th century. The British suppressed a native uprising in 1795. It was an associate state of Great Britain 1967-74 but became an independent nation within the British Commonwealth in 1974. The original name referred to the Feast of the Immaculate Conception but it was later renamed for the Spanish kingdom of Granada. (From Webster's New Geographical Dictionary, 1988, p467 & Room, Brewer's Dictionary of Names, 1992, p219)
A genus of gram-negative, anaerobic, helical bacteria, various species of which produce RELAPSING FEVER in humans and other animals.
An infectious disease caused by a spirochete, BORRELIA BURGDORFERI, which is transmitted chiefly by Ixodes dammini (see IXODES) and pacificus ticks in the United States and Ixodes ricinis (see IXODES) in Europe. It is a disease with early and late cutaneous manifestations plus involvement of the nervous system, heart, eye, and joints in variable combinations. The disease was formerly known as Lyme arthritis and first discovered at Old Lyme, Connecticut.
A family of small, gram-negative organisms, often parasitic in humans and other animals, causing diseases that may be transmitted by invertebrate vectors.
Proteins found in any species of bacterium.
Infections or infestations with parasitic organisms. The infestation may be experimental or veterinary.
Diseases of domestic and wild horses of the species Equus caballus.
Animals considered to be wild or feral or not adapted for domestic use. It does not include wild animals in zoos for which ANIMALS, ZOO is available.
Suspensions of attenuated or killed bacteria administered for the prevention or treatment of infectious bacterial disease.
Large, hoofed mammals of the family EQUIDAE. Horses are active day and night with most of the day spent seeking and consuming food. Feeding peaks occur in the early morning and late afternoon, and there are several daily periods of rest.
The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)
Any of numerous agile, hollow-horned RUMINANTS of the genus Capra, in the family Bovidae, closely related to the SHEEP.
A genus of tick-borne protozoa parasitic in the lymphocytes, erythrocytes, and endothelial cells of mammals. Its organisms multiply asexually and then invade erythrocytes, where they undergo no further reproduction until ingested by a transmitting tick.
Infection of cattle, sheep, or goats with protozoa of the genus THEILERIA. This infection results in an acute or chronic febrile condition.
Enzyme that catalyzes the first step of the tricarboxylic acid cycle (CITRIC ACID CYCLE). It catalyzes the reaction of oxaloacetate and acetyl CoA to form citrate and coenzyme A. This enzyme was formerly listed as EC 4.1.3.7.

Restriction of major surface protein 2 (MSP2) variants during tick transmission of the ehrlichia Anaplasma marginale. (1/149)

Anaplasma marginale is an ehrlichial pathogen of cattle that establishes lifelong persistent infection. Persistence is characterized by rickettsemic cycles in which new A. marginale variant types, defined by the sequence of the expressed msp2 transcripts, emerge. The polymorphic msp2 transcripts encode structurally distinct MSP2 proteins and result in an antigenically diverse and continually changing A. marginale population within the blood. In this manuscript, we used sequence analysis of msp2 transcripts to show that a restricted repertoire of variant types, designated SGV1 and SGV2, is expressed within the tick salivary gland. The same SGV1 and SGV2 variant types were expressed in ticks regardless of the variant types expressed in the blood of infected cattle at the time of acquisition feeding by the ticks. Importantly, subsequent tick transmission to susceptible cattle resulted in acute rickettsemia composed of organisms expressing only the same SGV1 and SGV2 variant types. This indicates that the msp2 expressed by organisms within the tick salivary gland predicts the variant type responsible for acute rickettsemia and disease. This restriction of transmitted A. marginale variant types, in contrast to the marked diversity within persistently infected cattle, supports development of MSP2 vaccines to prevent acute rickettsemia in tick-transmitted infections.  (+info)

Biased immunoglobulin G1 isotype responses induced in cattle with DNA expressing msp1a of Anaplasma marginale. (2/149)

Immunization with the native major surface protein 1 (MSP1) (a heterodimer containing disulfide and noncovalently bonded polypeptides designated MSP1a and MSP1b) of the erythrocytic stage of Anaplasma marginale conferred protection against homologous challenge (G. H. Palmer, A. F. Barbet, W. C. Davis, and T. C. McGuire, Science 231:1299-1302, 1986). The MSP1a polypeptide possesses a conserved neutralization-sensitive epitope. In the present study, the immune response to DNA-mediated immunization using msp1a was studied. The plasmid pVCL/MSP1a, which encodes the complete msp1a gene of A. marginale under the control of human cytomegalovirus immediate-early enhancer/promoter and intron A, was constructed. The immune responses elicited by immunization with pVCL/MSP1a into cardiotoxin-induced regenerating muscle were evaluated in mice and cattle. Antibody reactive with native MSP1a was detected in pooled sera of immunized BALB/c mice 3 weeks following primary immunization. Two calves seronegative for A. marginale were immunized four times, at weeks 0, 3, 7, and 13, with pVCL/MSP1a. By 8 weeks, both calves responded to MSP1a with an antibody titer of 1:100, which peaked at 1:1,600 and 1:800 by 16 weeks after the initial immunization. Interestingly, immunoblotting with anti-immunoglobulin G1 (anti-IgG1) and anti-IgG2 specific monoclonal antibodies revealed a restricted IgG1 anti-MSP1a response in both animals. T-lymphocyte lines, established after the fourth immunization, proliferated specifically against A. marginale homogenate and purified MSP1 in a dose-dependent manner. These data provide a basis for an immunization strategy to direct bovine immune responses by using DNA vaccine vectors containing single or multiple genes encoding major surface proteins of A. marginale.  (+info)

Emergence of Anaplasma marginale antigenic variants during persistent rickettsemia. (3/149)

Anaplasma marginale is an ehrlichial pathogen of cattle, in the order Rickettsiales, that establishes persistent cyclic rickettsemia in the infected host. Within each rickettsemic cycle, A. marginale expressing antigenically variant major surface protein 2 (MSP2) emerge. By cloning 17 full-length msp2 transcripts expressed during cyclic rickettsemia, we determined that emergent variants have a single, central hypervariable region encoding variant B-cell epitopes. The N- and C-terminal regions are highly conserved among the expressed A. marginale variants, and similar sequences define the MSP2 homologues in the agent of human granulocytic ehrlichiosis (HGE). This is in contrast to the MSP2 homologues in ehrlichial genogroup I pathogens, Ehrlichia chaffeensis, Ehrlichia canis, and Cowdria ruminantium, that have multiple hypervariable regions. By defining the variable and conserved regions, we were able to show that the single hypervariable region of A. marginale MSP2 encodes epitopes that are immunogenic and induce variant-specific antibody responses during persistent infection. These findings demonstrate that the MSP2 structural variants that emerge during each cycle of persistent rickettsemia are true antigenic variants, consistent with MSP2 antigenic variation as a mechanism of A. marginale persistence.  (+info)

Interleukin-12 as an adjuvant promotes immunoglobulin G and type 1 cytokine recall responses to major surface protein 2 of the ehrlichial pathogen Anaplasma marginale. (4/149)

Anaplasma marginale is a tick-transmitted pathogen of cattle closely related to the human ehrlichiae, Ehrlichia chaffeensis and the agent of human granulocytic ehrlichiosis (HGE). These pathogens have in common a structurally conserved outer membrane protein (OMP) designated the major surface protein 2 (MSP-2) in A. marginale and HGE and OMP-1 in E. chaffeensis. Protective immunity against ehrlichial pathogens is believed to require induction of gamma interferon (IFN-gamma) and opsonizing immunoglobulin (Ig) subclasses directed against OMP epitopes that, in concert, activate macrophages for phagocytosis and killing. Because interleukin-12 (IL-12) acts as an adjuvant for protein immunization to induce IFN-gamma and protective immunity against intracellular pathogens, we hypothesized that as an adjuvant with MSP-2, IL-12 would augment type 1 recall responses to A. marginale. IL-12 was coadsorbed with MSP-2 to alum and shown to significantly enhance IFN-gamma production by lymph node cells (LNC) and LNC-derived CD4(+) T-cell lines from immunized calves following recall stimulation with A. marginale. LNC proliferation and IL-2 production were also enhanced in IL-12-treated calves. Elevated recall proliferative responses by peripheral blood mononuclear cells were still evident 9 months after immunization. Serum IgG levels were consistently increased in IL-12 immunized calves, predominantly due to higher IgG1 responses. The results support the use of IL-12 coadsorbed with OMP of ehrlichial pathogens in alum to amplify both antibody and type-1 cytokine responses important for protective immunity.  (+info)

Expression of polymorphic msp1beta genes during acute anaplasma Marginale rickettsemia. (5/149)

Immunization of cattle with native MSP1 induces protection against Anaplasma marginale. The native immunogen is composed of a single MSP1a protein and multiple, undefined MSP1b polypeptides. In addition to the originally sequenced gene, designated msp1beta(F1), we identified three complete msp1beta genes in the Florida strain: msp1beta(F2), msp1beta(F3), and msp1beta(F4). Each of these polymorphic genes encodes a structurally unique MSP1b protein, and unique transcripts can be identified during acute A. marginale rickettsemia. The structural polymorphism is clustered in discrete variable regions, and each MSP1b protein results from a unique mosaic of five variable regions. Although each of the MSP1b proteins in the Florida strain contains epitopes recognized by serum antibody induced by protective immunization with the native MSP1 complex, the variable regions also include epitopes expressed by some but not all of the MSP1b proteins. These data support testing recombinant vaccines composed of the multiple antigenically and structurally unique MSP1b proteins combined with MSP1a in order to mimic the efficacy of native MSP1 immunization.  (+info)

Strain diversity in major surface protein 2 expression during tick transmission of Anaplasma marginale. (6/149)

Specific major surface protein 2 (MSP2) variants are expressed by Anaplasma marginale within the tick salivary gland and, following transmission, are expressed during acute rickettsemia. In previous work, we have shown that a restricted pattern of MSP2 variants is expressed in the salivary glands of Dermacentor andersoni ticks infected with the South Idaho strain of A. marginale. Now we demonstrate that the identical restriction does not apply to two other strains of A. marginale, and that different variants are also expressed when the same strain is transmitted by different Dermacentor spp. This indicates that antigenic diversity among strains is maintained in tick transmission and may be a significant constraint to MSP2 vaccine development.  (+info)

Antigenic variation in vector-borne pathogens. (7/149)

Several pathogens of humans and domestic animals depend on hematophagous arthropods to transmit them from one vertebrate reservoir host to another and maintain them in an environment. These pathogens use antigenic variation to prolong their circulation in the blood and thus increase the likelihood of transmission. By convergent evolution, bacterial and protozoal vector-borne pathogens have acquired similar genetic mechanisms for successful antigenic variation. Borrelia spp. and Anaplasma marginale (among bacteria) and African trypanosomes, Plasmodium falciparum, and Babesia bovis (among parasites) are examples of pathogens using these mechanisms. Antigenic variation poses a challenge in the development of vaccines against vector-borne pathogens.  (+info)

Antigenic variation of Anaplasma marginale by expression of MSP2 mosaics. (8/149)

Anaplasma marginale is a tick-borne pathogen, one of several closely related ehrlichial organisms that cause disease in animals and humans. These Ehrlichia species have complex life cycles that require, in addition to replication and development within the tick vector, evasion of the immune system in order to persist in the mammalian reservoir host. This complexity requires efficient use of the small ehrlichial genome. A. marginale and related ehrlichiae express immunoprotective, variable outer membrane proteins that have similar structures and are encoded by polymorphic multigene families. We show here that the major outer membrane protein of A. marginale, MSP2, is encoded on a polycistronic mRNA. The genomic expression site for this mRNA is polymorphic and encodes numerous amino acid sequence variants in bloodstream populations of A. marginale. A potential mechanism for persistence is segmental gene conversion of the expression site to link hypervariable msp2 sequences to the promoter and polycistron.  (+info)

Anaplasma spp. are tick-transmitted bacteria that infect a wide variety of wild and domestic animals. These pathogens exhibit a high degree of biological diversity, broad geographical distribution, and represent a serious threat to veterinary and public health worldwide. A novel Anaplasma species was identified in Haemaphysalis qinghaiensis (Ixodidae) in northwestern China and was molecularly characterized by comparison of 16S rRNA, gltA, and groEL gene sequences. Of the 414 samples tested, 24 (5.8%) were positive for this Anaplasma species. On the basis of the 16S rRNA gene, this organism has been found to be closely related to and exhibit the highest sequence similarity with A. capra (99.8-99.9%) that was identified in goats and humans in northern China, but was distinct from other known Anaplasma species. Sequence analysis of the gltA and groEL genes revealed that this Anaplasma species was distinct from A. capra considering the lower sequence identity (88.6-88.7% for gltA
The genus Anaplasma (Rickettsiales: Anaplasmataceae) comprises obligate intracellular Gram-negative bacteria that are mainly transmitted by ticks, and currently includes six species: Anaplasma bovis, Anaplasma centrale, Anaplasma marginale, Anaplasma phagocytophilum, Anaplasma platys, and Anaplasma ovis. These have long been known as etiological agents of veterinary diseases that affect domestic and wild animals worldwide. A zoonotic role has been recognized for A. phagocytophilum, but other species can also be pathogenic for humans.
Anaplasma species infection mimics many other conditions, including other chronic infections. If you are experiencing hard-to-diagnose chronic symptoms and have had a recent tick attachment or live in a region endemic for Anaplasma species in ticks, you should consider testing for Anaplasma infection. Symptoms can range from mild to serious illness characterized by fever, chills, headache, confusion, myalgia, nausea/vomiting/diarrhea, acute weight loss, abdominal pain, difficulty breathing, neurological problems and other conditions that may require immediate medical attention. This zoonotic infection is primarily transmitted to humans by Ixodes ticks, but can also be transmitted through blood transfusion. Clinical presentations may be more severe when co-infected with multiple tick-borne pathogens.. Tests Offered. Anaplasma species PCR test is designed to detect all pathogenic Anaplasma species. Positive results are confirmed by DNA sequencing to identify the infecting species. It is ...
Anaplasma spp. are widely spread rickettsial bacteria transmitted by ticks and placing high impacts on veterinary and public health. A limited number of studies have been carried out on Anaplasmosis in the central part of Iraq. This study was conducted to determine the presence of Anaplasma spp. in cattle in Al-Qadisiyah province, Iraq. A total of 400 blood specimens were collected from cattle suffering from heavy tick infestation. Cattle were blood-sampled from four hyper-endemic areas with ticks. Blood samples were screened using microscopic and polymerase chain reaction (PCR) methods. Diff-quick stained blood smears revealed Anaplasma-like inclusion bodies in 254 (63.5%) samples. According to the 16S rRNA-gene-based PCR analysis, Anaplasma spp. was detected in 124 of the 400 (31%) samples, divided as 96/254 (37.8%) among the microscopical positive samples and 28/146 (19.17%) among the microscopical negative samples. Phylogenetic analysis based on the partial 16S rRNA gene sequencing of ...
Shop Major surface protein ELISA Kit, Recombinant Protein and Major surface protein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Les Anaplasmataceae sont des bactéries intracellulaires transmises aux animaux et à lhomme, principalement par les tiques. Les Anaplasma spp. (A. marginale, A. phagocytophilum…) et les Ehrlichia spp. (E. canis, E. ruminantium...) en sont les principaux représentants dimportance économique et sanitaire. Les outils de diagnostic disponibles jouent un rôle déterminant dans leur identification. Nous proposons une approche globale par un système permettant didentifier la plupart des espèces dAnaplasmataceae. Il est basé sur le gène codant lARNr 23S ; il couple une qPCR et une PCR standard avec deux jeux damorces suivie dun séquençage. Il est testé pour en vérifier la spécificité, puis mis en oeuvre à partir de prélèvements de sang de ruminants et de tiques : dans les Pyrénées-Atlantiques, des brebis suspectes dinfection sont porteuses dA. ovis et leurs tiques Rhipicephalus bursa, dA. ovis, dA. phagocytophilum et dune nouvelle ehrlichia. En Haute-Corse, une forte ...
Clinical assay intended to detect the presence of Babesia and Anaplasma species in whole blood specimens.. If screening test is positive or equivocal, the test will automatically be referred for Lyme Disease Antibody, Confirmation, Serum testing at an additional charge ...
Classification of bacteria is challenging due to the lack of a theory-based framework. In addition, the adaptation of bacteria to ecological niches often results in selection of strains with diverse virulence, pathogenicity and transmission characteristics. Bacterial strain diversity presents challenges for taxonomic classification, which in turn impacts the ability to develop accurate diagnostics and effective vaccines. Over the past decade, the worldwide diversity of Anaplasma marginale, an economically important tick-borne pathogen of cattle, has become apparent. The extent of A. marginale strain diversity, formerly underappreciated, has contributed to the challenges of classification which, in turn, likely impacts the design and development of improved vaccines. Notably, the A. marginale surface protein 1a (MSP1a) is a model molecule for these studies because it serves as a marker for strain identity, is both an adhesin necessary for infection of cells and an immuno-reactive protein and is ...
Vector-borne pathogens are emerging concerns in multiple regions of Canada. Determining regional prevalence of canine vector-borne pathogens and documenting change will improve clinician awareness, enable targeted prevention, enhance diagnosis and ideally reduce the risk of disease. Study objectives were to: (i) estimate the prevalence of positive canine vector-borne test results from samples submitted in Canada; (ii) assess change in prevalence over time, from baseline (2008) to 2015; and (iii) estimate the prevalence of pathogen co-infections. This repeat cross-sectional study evaluated 753,468 test results for D. immitis antigen and B. burgdorferi, Ehrlichia canis/ewingii/muris serology, and 753,208 test results for Anaplasma phagocytophilum/platys serology using the SNAP® 4Dx®Test and SNAP 4Dx® Plus Test. Based on all submitted samples from Canada (2008-2015), the period seroprevalence of B. burgdorferi, Ehrlichia spp., Anaplasma spp. and D. immitis antigen were 2.0%, 0.5%, 0.4% and 0.2%,
Tick-borne pathogens are of great importance for small animal clinics and public health since they are increasingly prevalent among dogs and can infect humans (Jojima et al. 2008Jojima F.S., Garcia J.L., Vidotto M.C., Balarin M.R., Fabretti A.K., Gasparini M.R., Coelho A.L. & Vidotto O. 2008. Occurrence and molecular characterization of Babesia species in a canine hospital population in the Londrina Region, Parana State, Brazil. Revta Bras. Parasitol. Vet. 1:277-283., Maggi et al. 2013Maggi R.G., Mascarelli P.E., Havenga L.N., Naidoo V. & Breitschwerdt E.B. 2013. Co-infection with Anaplasma platys, Bartonella henselae and Candidatus Mycoplasma haematoparvum in a veterinarian. Parasit. Vectors 6:103.). In Brazil, there is lack of information about the distribution of hemoparasites and their vectors. This is the first study that evaluates the prevalence of Anaplasma platys, Ehrlichia canis and Babesia vogeli among dogs not domiciled in the southwestern region of Paraná, South of Brazil.. All ...
The establishment and maintenance of anaplasmosis-free cattle herds is impaired due to the lack of a rapid, sensitive, and specific serologic test to detect persistently infected cattle which serve as carriers for the organism. To develop an improved diagnostic test for anaplasmosis we screened Anaplasma marginale initial body proteins to identify a protein common to antigenically different isolates that is recognized by the host immune system at all stages of infection. Seronegative cattle were infected with either the Florida, Virginia, or North Texas isolate of A. marginale and monitored for infection by daily examination of Wright-stained blood smears for parasitized erythrocytes. Sera from cattle at different stages of infection, from acute through persistent, were used to immunoprecipitate A. marginale proteins that were metabolically radiolabeled with [35S]methionine or surface radiolabeled with 125I. Multiple A. marginale proteins were recognized by using sera either undiluted or at ...
A major surface protein complex from the Florida isolate of Anaplasma marginale has been previously shown to induce protection in immunized cattle and has been proposed as the basis of a subunit vaccine against anaplasmosis. This complex in the Florida isolate is composed of two noncovalently associated polypeptides with molecular masses of 105 and 100 kilodaltons (kDa). The analogous protein complex from four geographically different isolates of A. marginale was immunoprecipitated and compared with the protein complex of the Florida isolate. The polypeptides of the complex varied in apparent molecular mass among the isolates. By using antibodies recognizing epitopes on each polypeptide of the Florida isolate, the antigenic identity of the polypeptides in the analogous complexes was determined. The polypeptides recognized by the neutralizing monoclonal antibody 22B1, which recognizes a 105-kDa polypeptide in the Florida isolate, ranged from 70 to 100 kDa in the other isolates. Those polypeptides ...
TY - JOUR. T1 - Prevalence of human-Active and variant 1 strains of the tick-borne pathogen Anaplasma phagocytophilum in hosts and forests of Eastern North America. AU - Keesing, Felicia. AU - McHenry, Diana J.. AU - Hersh, Michelle. AU - Tibbetts, Michael. AU - Brunner, Jesse L.. AU - Killilea, Mary. AU - LoGiudice, Kathleen. AU - Schmidt, Kenneth A.. AU - Ostfeld, Richard S.. PY - 2014. Y1 - 2014. N2 - Anaplasmosis is an emerging infectious disease caused by infection with the bacterium Anaplasma phagocytophilum. In the eastern United States, A. phagocytophilum is transmitted to hosts through the bite of the blacklegged tick, Ixodes scapularis. We determined the realized reservoir competence of 14 species of common vertebrate hosts for ticks by establishing the probability that each species transmits two important strains of A. phagocytophilum (A. phagocytophilum human-Active, which causes human cases, and A. phagocytophilum variant 1, which does not) to feeding larval ticks. We also sampled ...
Shared and unique antigens were identified and partially characterized for two morphologically and antigenically distinct isolates of Anaplasma, A. marginale (Florida) (FAM) and A. caudatum (Illinois) (IAC). Crude CA-like antigens were prepared from the parasitemic blood of each calf, separated by SDS-PAGE, electroblotted onto nitrocellulose, and detected using immune bovine sera and an avidin-biotin-peroxidase assay with biotinylated rabbit anti-goat IgG (cross-reacted with cattle, sheep and deer antibodies). IAC antigens were 200 kilodalton (kd), 100 kd, 96 kd, 75 kd, 47 kd, 43 to 38 kd, and 27 kd, detected using anti-IAC and anti-FAM sera. FAM antigens were 108 kd, 100 kd, 96 kd, 91 kd, 75 kd, 47 kd, 43 to 38 kd, and 27 kd, detected using anti-FAM sera and, except for the 91 kd, with anti-IAC sera. The 91 kd is an FAM isolate-specific antigen. The 108 kd, 100 kd, and 96 kd are group-specific antigens of A. marginale and A. centrale. The 43 to 38 kd is genus-specific. The 96, 91, and 75 kd ...
The University of Missouri Veterinary Medical Diagnostic Laboratory (VMDL) is a full-service veterinary diagnostic laboratory and Missouris only laboratory accredited by the American Association of Veterinary Laboratory Diagnosticians. The VMDL is a Level I Lab of the National Animal Health Laboratory Network, Tier I Lab of the FDA Veterinary Laboratory Response Network, and a National Poultry Improvement Plan authorized lab. The VMDL maintains an effective quality assurance and quality control program. The VMDL has formal anatomical pathology, clinical pathology, and clinical microbiology training programs. The VMDL faculty hold academic appointments in the Departments of Veterinary Pathobiology and Veterinary Biomedical Sciences. The director is administratively responsible to the dean of the College of Veterinary Medicine for the overall operation of the laboratory and its multiple missions of public service, teaching, research and continuing education/extension. The VMDL faculty and staff ...
BACKGROUND: Only a few studies have examined the presence of Anaplasma marginale and Anaplasma centrale in South Africa, and no studies have comprehensively examined these species across the whole country. To undertake this country-wide study we adapted a duplex quantitative real-time PCR (qPCR) assay for use in South Africa but found that one of the genes on which the assay was based was variable ...
24.BraytonKA,PalmerGH,BrownWC: Genomicandproteomicapproaches tovaccinecandidateidentificationfor Anaplasmamarginale ExpertRev Vaccines 2006, 5: 95 101. 25.PierleSA,DarkMJ,DahmenD,PalmerGH,BraytonKA: Comparative genomicsandtranscriptomicsoftrait-geneassociation. BMCGenomics 2012, 13: 669. 26.VanOpijnenT,CamilliA: Transposoninsertionsequencing:anewtoolfor systems-levelanalysisofmicroorganisms. NatRevMicrobiol 2013, 11: 435 442. 27.ClaeysBouuaertC,ChalmersRM: Genetherapyvectors:theprospectsand potentialsofthecut-and-pastetransposons. Genetica 2010, 138: 473 484. 28.PicardeauM: Transpositionofflymarinerelementsintobacteriaasa genetictoolformutagenesis. Genetica 2010, 138: 551 558. 29.LampeDJ,GrantTE,RobertsonHM: Factorsaffectingtranspositionofthe Himar1 marinertransposoninvitro. Genetics 1998, 149: 179 187. 30.PlasterkRH,IzsvakZ,IvicsZ: Residentaliens:theTc1/marinersuperfamily oftransposableelements. TrendsGenet 1999, 15: 326 332. 31.ClarkTR,EllisonDW,KlebaB,HackstadtT: Complementationof Rickettsia ...
Ubiquinone (UQ), also called coenzyme Q, and plastoquinone (PQ) are electron carriers in oxidative phosphorylation and photosynthesis, respectively. The quinoid nucleus of ubiquinone is derived from the shikimate pathway; 4-hydroxybenzoate is directly formed from chorismate in bacteria, while it can be formed from either chorismate or tyrosine in yeast. The following biosynthesis of terpenoid moiety involves reactions of prenylation, decarboxylation, and three hydroxylations alternating with three methylations. The order of these reactions are somewhat different between bacteria and yeast. Phylloquinone (vitamin K1), menaquinone (vitamin K2), and tocopherol (vitamin E) are fat-soluble vitamins. Phylloquinone is a compound present in all photosynthetic plants serving as a cofactor for photosystem I-mediated electron transport. Menaquinone is an obligatory component of the electron-transfer pathway in bacteria ...
BLOT kit for the detection of specific IgG antibodies to the recombinant antigens of Anaplasma phagocytophilum (HGA) in human serum and plasma.
What is Anaplasmosis? Anaplasmosis is a disease caused by Anaplasma spp, a rickettsial parasite of ruminants, and is thus associated to rickettsial disease. Gram-negative microorganisms invade red blood cells and invade them. They are spread by a variety of haematophagous… Continue Reading → ...
Lineage: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales; Anaplasmataceae; Anaplasma; Anaplasma ...
Lineage: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales; Anaplasmataceae; Anaplasma; Anaplasma ...
A population of approximately 2500 dogs inhabits the island of Santa Cruz in the Galápagos Islands and many of these dogs freely roam the streets of the island in close proximity to people. The dog population receives minimal, if any, veterinary care or ectoparasite prophylaxis, leaving the majority of the dogs at risk for vector-borne infections such as Anaplasma platys, Borrelia burgdorferi, Dir ...
(34) Grouped under this heading are chlamydia, mycoplasma and other bacteria generally. Chlamydia These and anaplasma used to be classified separately from bacteria but are now...
I have a 20g with 3 platys and a BN. Recently I bought some live plants from Petco. They came with snails and within 3 days I had 2 dead female platys with what
2002) Erratum to A msp1α polymerase chain reaction assay for specific detection and differentiation of Anaplasma marginale isolates [Vet. Microbiol. 86 (2002) 325-335]. Veterinary Microbiology, 87 (4). pp. 365-366. ...
DETECTION OF Anaplasma marginale INFECTION IN A DAIRY CATTLE FARM BY STAINED BLOOD SMEAR EXAMINATION AND NESTED POLYMERASE CHAIN REACTION
Anaplasma marginale is the most prevalent tick-borne pathogen of cattle. It is transmitted by ixodid ticks. Symptoms of acute disease are anemia, weight loss, and often death. A.marginale establishes life-long persistent infection in animals that survive disease. These animals are clinically healthy but serve as reservoirs for transmission of the pathogen. Anaplasma marginale subsp. centrale (Anaplasma centrale (strain Israel)) is a naturally occurring attenuated strain has been used as a live vaccine to prevent severe disease due to A. marginale senso stricto strains for 100 years. A. marginale subsp. centrale contains 10 putative genes not found in the genomes of senso stricto strains, while 18 genes found in senso stricto strains are absent from A. marginale subsp. centrale. As inoculation with purified outer membrane protein (OMP) complexes provides immunity against A. marginale, it is important to identify OMPs that differ among A,marginale to identify further vaccine candidates. ...
TY - JOUR. T1 - Murine neutrophils require α 1,3-fucosylation but not PSGL-1 for productive infection with Anaplasma phagocytophilum. AU - Carlyon, Jason A.. AU - Akkoyunlu, Mustafa. AU - Xia, Lijun. AU - Yago, Tadayuki. AU - Wang, Tian. AU - Cummings, Richard D.. AU - McEver, Rodger P.. AU - Fikrig, Erol. PY - 2003/11/1. Y1 - 2003/11/1. N2 - Anaplasma phagocytophilum causes human granulocytic ehrlichiosis, the second most common tick-borne disease in the United States. Mice are natural reservoirs for this bacterium and man is an inadvertent host. A phagocytophilums tropism for human neutrophils is linked to neutrophil expression of P-selectin glycoprotein ligand-1 (PSGL-1), as well as sialylated and α1,3-fucosylated glycans. To determine whether A phagocytophilum uses similar molecular features to infect murine neutrophils, we assessed in vitro bacterial binding to neutrophils from and infection burden in wild-type mice; mice lacking α1,3-fucosyltransferases Fuc-TIV and Fuc-TVII; or mice ...
Clinical. Epidemiological elements + Clinical signs. Laboratory. Direct. Using light microscopy on peripheral stained blood smears, the diagnosis relies on the observation of a typical morula of Anaplasma platys in the platelets for thrombocytic anaplasmosis and the observation of a typical morula of Anaplasma phagocytophilum in the neutrophils for granulocytic anaplasmosis.. Infection is confirmed by PCR detection of bacterial DNA on tissues or blood.. Indirect. Indirect fluorescent antibody (IFA) assays to determine serum antibody against Anaplasma spp. available in veterinary laboratories.. Fast diagnosis at patient-side, based on an multivalent enzyme-linked immunosorbent assay (ELISA) is available for the detection of antibodies to E. canis, E. ewingii, A. phagocytophilum and A. platys.. Differential. Other tick-borne pathogens: Ehrlichia spp., Borrelia spp., Rickettsia spp.. Co-infection with other vector-borne pathogens is reported, especially co-infection with B. burgdorferi and A. ...
Anaplasma phagocytophilum is a zoonotic and obligate intracellular bacterium transmitted by ticks. In domestic ruminants, it is the causative agent of tick-borne fever, which causes significant economic losses in Europe. As A. phagocytophilum is difficult to isolate and cultivate, only nine genome sequences have been published to date, none of which originate from a bovine strain. Our goals were to; 1/ develop a sequencing methodology which efficiently circumvents the difficulties associated with A. phagocytophilum isolation and culture; 2/ describe the first genome of a bovine strain; and 3/ compare it with available genomes, in order to both explore key genomic features at the species level, and to identify candidate genes that could be specific to bovine strains. DNA was extracted from a bovine blood sample infected by A. phagocytophilum. Following a whole genome capture approach, A. phagocytophilum DNA was enriched 197-fold in the sample and then sequenced using Illumina technology. In total, 58.9%
Tick-borne pathogens cause emerging zoonoses, and include fastidious organisms such as Anaplasma phagocytophilum. Because of their obligate intracellular nature, methods for mutagenesis and transformation have not been available. To facilitate genetic manipulation, we transformed A. phagocytophilum (Ap) to express a green fluorescent protein (GFP) with the Himar1 transposase system and selection with the clinically irrelevant antibiotic spectinomycin. These transformed bacteria (GFP/Ap) grow at normal rates and are brightly fluorescent in human, monkey, and tick cell culture. Molecular characterization of the GFP/Ap genomic DNA confirmed transposition and the flanking genomic insertion locations were sequenced. Three mice inoculated with GFP/Ap by intraperitoneal injection became infected as demonstrated by the appearance of morulae in a peripheral blood neutrophil and re-isolation of the bacteria in culture.
Nango is neutered (April-12-2015), up to date with routine shots, micro-chipped, de-wormed and tested negative for heartworm.. Was likely abandoned as a puppy, when found collar outgrew causing neck laceration: Debridement and suture performed 04-13-2015 with full recovery. Blood Test January-25-2018. X-Rays January-25-2018. Patellar Luxation Examination January-25-2018. Dental Cleaning January-25-2018. Idexx SNAP® 4Dx® (April-11-2015). Tested negative for Heartworm, Lyme, Ehrlichia Canis, and Anaplasma Phagocytophilum. Polymerase Chain Reaction PCR Test (May-14-2015). Tested negative for Babesia spp. (B. Canis and B. Gibsoni), Ehrlichia spp. (E. Canis and Anaplasma Platys), Haemobartonella Canis, and Borrelia Burgdorferi. ...
Background: Tick-borne bacteria and protozoa can cause a variety of human and animal diseases in China. It is of great importance to monitor the prevalence and dynamic variation of these pathogens in ticks in ever-changing natural and social environment. Materials and Methods: Ticks were collected from Heilongjiang and Jilin provinces of northeastern China during 2018-2019 followed by morphological identification. The presence of Rickettsia spp., Anaplasma spp., Ehrlichia spp., Borrelia spp., Babesia spp., and Theileria spp. was examined by PCR and Sanger sequencing. The obtained sequences were subjected to phylogenetic analysis through Mega 7.0. Statistical analysis was performed using SPSS 24.0. Results: A total of 250 ticks from 5 species of 3 genera were collected. Ixodes and Haemaphysalis ticks carried more species of pathogens than Dermacentor, and the pathogens detected in Haemaphysalis japonica varied significantly among different sampling sites. The infection rates of Rickettsia spp., ...
Azad, AF., Radulovic, S., Higgins, JA., Noden, BH., Troyer, JM., 1997. Flea-borne rickettsioses: ecologic considerations. Emerg. Infect. Dis. 3, 319-327.. Beck, W., Boch, K., Mackensen, H., Wiegand, B. and Pfister, K., 2006 Qualitative and quantitative observations of the flea population dynamics of dogs and cats in several areas in Germany. Veterinary Pararsitology, 137, 130-136.. Bermann, F., Davoust, B., Fournier, P.E., Brisou-Lapointe, A.V., Brouqui, P., 2002. Ehrlichia equi (Anaplasma phagocytophila) infection in an adult horse in France. Vet. Rec., 150, 787-788.. Beugnet, F., Kenny, MJ., Day, MJ. , Shaw, SE. A PCR-based comparative survey of arthropod-transmitted infections in dogs, cats and prevalence in ticks in southern France. Proceedings, WAAVP 2003, New Orleans.. Beugnet, F., Loukos, H., Chalvet-Monfray, K., Bicout, D., 2008. FleaTickRisk : a climatic model developed to monitor and predict the activity and the density of 3 ticks species and the cat flea in France. Proceedings EMOP X, ...
Anaplasma marginale is a tick-transmitted, obligate intraerythrocytic pathogen of cattle, causing anaplasmosis characterized by anemia, decreased milk and meat production, and occasionally death. Animals that survive are ...
Ha, S., Furukawa, R., Stramiello, M., Wagner, J.J., and Fechheimer, M. (2011). Transgenic Mouse Model for the Formation of Hirano Bodies. BMC Neurosci. 12:97. [Epub ahead of print]. Ha, S., Furukawa, R., and Fechheimer, M. (2011). Association of AICD and Fe65 with Hirano bodies reduces transcriptional activation and initiation of apoptosis. Neurobiol Aging. 32:2287-98.. Kim, D.-H., R. Furukawa, and M. Fechheimer. 2009. Degradation of Hirano Bodies by Autophagy 5, 44-51.. Davis, R. C., Furukawa, R., and M. Fechheimer, 2008. A Mammalian Cell Culture Model for the Formation of Hirano Bodies, Acta Neuropathologica 115, 205-217.. Stich, R. W., G. A. Olah, K. A. Brayton, W. C. Brown, M. Fechheimer, K. Green-Church, S. Jittapalapong, K. M. Kocan, T. C. McGuire, F. R. Rurangirwa, and G. H. Palmer. 2004. Identification of a novel Anaplasma marginale appendage-associated protein that localizes with actin filaments during intraerythrocytic infection. Infection and Immunity 72, 7257-7264.. Maselli, A., R. ...
In der vorliegenden in vitro Studie sollte der Frage nachgegangen werden, inwieweit die Teilkronenpräparation Einfluss auf die marginale Integrität der vollkeramischen Restauration im Schmelz und im Dentin nach thermomechanischer Wechselbelastung hat. Es wurden zwei unterschiedliche Präparationsformen gewählt, wobei der vestibuläre, nichttragende Höcker auf 1,0 mm Restzahnhartsubstanzstärke ausgedünnt (Präparation A) oder zusätzlich um ca. 2,0 mm horizontal reduziert und überkuppelt (Präparation B) wurde. Von den Zähnen wurden vor und nach thermomechanischer Wechselbelastung Epoxidharzreplikate hergestellt, an denen die marginale Integrität computergestützt bei 201 facher Vergrößerung mittels rasterelektronenmikroskopischer Randanalyse sowohl an allen Grenzen (Schmelz/Befestigungskomposit, Dentin/Befestigungskomposit und Keramik/Befestigungskomposit), als auch an allen Flächen (okklusal/vestibulär, palatinal, approximal/mesial und approximal/distal) ermittelt wurde. Bei ...
It is defined as a disorder in which there is an infection caused by the bacteria. It resides in the cell and is known as the Anaplasma phago cyto philum.
I wanted to pass a little information to you all that cache with your pets, and also to make you aware of this as it can also affect humans. My dog was in serious joint pain and overall tenderness this week, lack of energy, and shivering at times. My first thought was lyme disease, as she has already experienced that quite a few years ago. Off to the vet we go, only to find out after blood work and testing in the office, that it is not lyme, but another tick issue called anaplasma. This is
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Prices - Initial Exam - $70.00 +Toe Nail Trim - $12.50Vaccine Exam - $70.00 +Microscopic Fecal Analysis - $48.004dx Test (hwt,lymes, tick fever, anaplasma) -
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I had thought to linger around the house in my Jammies and not do much, except possibly go buy some female platys because the lone female does not appreciate their attentions ...
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http://publications.ki.se/xmlui/discover?query=978-91-7409-752-8&submit=S%C3%B6k. 2011-10 Specialist in stomatognathic physioloogy (orofacial pain), Eastmaninstitutet Folktandvården AB. 2012-02 Doctoral Supervisor Training course, Karolinska Institutet. 2012-12 Teaching and learning in Higher Education, Karolinska Institutet. 2016-05 Specialist in Advanced Pain Medicine, Scandinavian Society of Anaesthesiology and Intensive Care Medicine (SSAI). 2017-01 Associate professor in odontology, Karolinska Institutet. ...
Serological surveys have confirmed Anaplasma marginale and Anaplasma phagocytophilum infections in dromedary camels, but molecular surveys and genetic characterisation of camel-associated Anaplasma species are lacking. In this study, we detected tick-borne Anaplasmataceae in 30 of 100 (30%) healthy dromedary camels screened using a combined 16S rRNA-groEL PCR-sequencing approach. Nucleotide sequencing confirmed Anaplasmataceae genome presence in 28 of the 33 16S rRNA PCR-positive samples, with two additional positive samples, for which 16S rRNA sequence data were ambiguous, being identified by groEL gene characterisation. Phylogenetic analyses of a 1289 nt segment of the 16S rRNA gene confirmed the presence of a unique Ehrlichia lineage and a discrete Anaplasma lineage, comprising three variants, occurring at an overall prevalence of 4% and 26%, respectively. Genetic characterisation of an aligned 559 nt groEL gene region revealed the camel-associated Anaplasma and Ehrlichia lineages to be novel ...
The genus Anaplasma consists of tick-transmitted obligate intracellular bacteria that invade white or red blood cells to cause debilitating and potentially fatal infections. A. phagocytophilum, a human and veterinary pathogen, infects neutrophils to cause granulocytic anaplasmosis. A. marginale invades bovine erythrocytes. Evidence suggests that both species may also infect endothelial cells in vivo. In mammalian and arthropod host cells, A. phagocytophilum and A. marginale reside in host cell derived pathogen-occupied vacuoles (POVs). While it was recently demonstrated that the A. phagocytophilum-occupied vacuole (ApV) intercepts membrane traffic from the trans-Golgi network, it is unclear if it or the A. marginale-occupied vacuole (AmV) interacts with other secretory organelles. Here, we demonstrate that the ApV and AmV extensively interact with the host endoplasmic reticulum (ER) in endothelial, myeloid, and/or tick cells. ER lumen markers, calreticulin and protein disulfide isomerase, and the ER
A TaqMan real-time PCR assay targeting the Anaplasma citrate synthase gene, gltA, was developed and used for detection of Anaplasma phagocytophilum in 765 Ixodes ricinus ticks collected from dogs and cats in northern Norway (n = 669) and Telemark county in southern Norway (n = 96). Among the ticks from northern Norway the prevalence of A. phagocytophilum was 3.0 %, while the prevalence in southern Norway was 2.1 % (p = 0.63). The gltA PCR assay showed a high analytical sensitivity (30 genomic units) and efficiency (98.5 %), and its utility in clinical diagnostics should be evaluated in future studies. This is the first report of A. phagocytophilum occurrence in ticks collected north of the Arctic Circle in Norway. The prevalence is comparable to that found in Telemark county in southern Norway.
Fourteen vertebrate species (10 mammals and 4 birds) were assessed for their ability to transmit Anaplasma phagocytophilum, the bacterium that causes human granulocytic anaplasmosis, to uninfected feeding ixodid ticks. Small mammals were most likely to infect ticks but all species assessed were capable of transmitting the bacterium, in contrast to previous findings.
Human granulocytic anaplasmosis (HGA) is an emerging tick-borne disease caused by Anaplasma phagocytophilum, an obligate intracellular bacterium of neutrophils....
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Drovers. Molecular biologist Susan Noh, at the Agricultural Research Services Animal Disease Research Unit in Pullman, Washington, is working to develop a vaccine to protect against anaplasmosis, a tick-transmitted disease of cattle. Caused by the microbe Anaplasma marginale, anaplasmosis affects cattle health, well-being, and production in many parts of the world and is characterized by severe anemia, fever, and weight loss. Despite this threat, there is no widely accepted vaccine for anaplasmosis.. Through their studies, Noh and her colleagues at Washington State University have identified important proteins to include in a potential vaccine, which is now being tested on animals. They found that small groups of the outer surface proteins of A. marginale induce an immune response that not only reduces symptoms, but can also prevent A. marginale infection in some animals. Some of the more promising vaccines being tested have protected 80 to 90 percent of the animals from clinical disease and ...
George Garrity, Don J. Brenner, James T. Staley, Noel R. Krieg, David R. Boone, Paul De Vos, Michael Goodfellow, Fred A. Rainey, Karl-Heinz Schleifer, ...,Bergeys Manual® of Systematic Bacteriology: Volume Two: The Proteobacteria, lk 123,2005,Googlei raamat veebiversioon (vaadatud 21.01.2014) (inglise keeles) ...
Cel mai adeasea, cei care locuiesc la bloc isi achizitioneaza si monteaza centrale termice cu condensare datorita faptului ca acestea consuma putina energie.. Nu trebuie sa mergi neaparat prin magazine si sa umbli zile in sir pentru a cumpara o centrala. Poti cumpara o centrala si online de pe Epinvest.ro. Intra pe site si consulta lista cu centralele disponibile.. Nu este convenabil sa cumperi centrale termice cu condensare atunci cand locuiesti la casa. Daca locuiesti la casa, cel mai convenabil este sa-ti achizitionezi o centrala termica pe lemne. Ai cu siguranta spatiu suficient pentru depozitarea lemnelor si suficient spatiu pentru camera in care tii centrala.. ...
La Conférence sur le diabète et les autres MNT en Afrique de lOuest et en Afrique centrale a examiné les défis que le diabète et les autres maladies non transmissibles représentent pour la santé et le bien-être des citoyens de la région et par conséquent pour les économies de ces pays. Les participants à cette rencontre ont adopté la déclaration suivante et ont lancé un appel urgent à laction ...
En soutien aux secteurs de la culture et de la création en Afrique centrale. Projet mis en oeuvre par un consortium de partenaires africains et européens.
Paul Salkin - Alors que les tensions russo-allemandes sont à leur comble en Europe, Cobourg et Hanovre, ethnologues de sang royal, débarquent au XXIe siècle dans un Congo qui entre dans lère des indépendances.
Red is the color of excitement, fierceness, and happiness. In Asian countries, it is also a symbol of luck and prosperity. Among fishes, the species that has a... Read More... ...
Baneth, G. (2010). Ehrlichia and anaplasma infections. Paper presented at World small animal veterinary congress. Retrieved ...
Anaplasma phagocytophilum causes granulocytic anaplasmosis. Borrelia burgdorferi causes Lyme disease. Rickettsia rickettsii ...
December 2005). "Human granulocytic anaplasmosis and Anaplasma phagocytophilum". Emerging Infectious Diseases. 11 (12): 1828-34 ... and Anaplasma phagocytophilum in ticks (Acari: Ixodidae) from a coastal region of California". J. Med. Entomol. 40 (4): 534-9. ...
... are found in Anaplasma and those which interact with Anaplasma can mainly be found in A. marginale and A. phagocytophilum. ... Anaplasma phagocytophilum shares its tick vector with other human pathogens, and about 10% of patients with HGA show serologic ... Anaplasma MSPs can not only cooperate with vertebrates, but also invertebrates, which make these phenotypes evolve faster than ... Lee HC, Kioi M, Han J, Puri RK, Goodman JL (September 2008). "Anaplasma phagocytophilum-induced gene expression in both human ...
Rickettsiales (Rickettsia, Ehrlichia, Anaplasma, Coxiella) Mycoplasma spp. Levinson, Warren (2018). Review of medical ...
136). Mackerras, I. M., Mackerras, M. J., & Mulhearn, C. R. (1942). Attempted transmission of Anaplasma marginale Theiler by ...
"mCherry - MCherry fluorescent protein - Anaplasma marginale - mCherry gene & protein". www.uniprot.org. Retrieved 2018-11-11. ...
Strains of Anaplasma bovis have also been found in rabbit ticks, which was discovered in a Nantucket tick study of the bacteria ... Goethert, H.K.; S.R. Telford III (2003). "Enzootic Transmission of Anaplasma bovis in Nantucket Cottontail Rabbits". Journal of ...
21: Rickettsia, Orientia, Ehrlichia, Anaplasma, Coxiella and Bartonella". Microbiology and Immunology On-line. University of ...
T4SS has two effector proteins: firstly, ATS-1, which stands for Anaplasma translocated substrate 1, and secondly AnkA, which ... Rikihisa Y, Lin M, Niu H (September 2010). "Type IV secretion in the obligatory intracellular bacterium Anaplasma ...
The two major species that cause anaplasmosis in ruminants include Anaplasma marginale and Anaplasma phagocytophilum. Anaplasma ... Anaplasma phagocytophilium has a prevalence of 11.9% in sheep, and 15.2% in goats. There are many strains of Anaplasma ... The two major bacterial pathogens are Anaplasma marginale and Anaplasma phagocytophilum. These microorganisms are Gram-negative ... Atif FA (November 2015). "Anaplasma marginale and Anaplasma phagocytophilum: Rickettsiales pathogens of veterinary and public ...
... and Anaplasma". Topics in Companion Animal Medicine. 26 (4): 173-7. doi:10.1053/j.tcam.2011.09.002. PMID 22152604. Callaway, ...
anaplasmosis Infection with Anaplasma, a genus of Sporozoa that infests red blood cells. anasa wilt A wilt disease of cucurbits ...
Like Anaplasma phagocytophilum, the causative agent of human granulocytic ehrlichiosis, Ehrlichia ewingii infects neutrophils. ...
Evidence of Anaplasma marginale Transmission by Sucking Lice Haematopinus tuberculatus". Journal of Parasitology. 99 (3): 546- ...
... and its closely related genus Anaplasma show extreme diversity in the structure and content of their genomes. This ... "Structural basis for segmental gene conversion in generation of Anaplasma marginale outer membrane protein variants". Molecular ...
... and the bacterium Anaplasma marginale. Horses may be infected with Lyme disease, Anaplasma phagocytophila, and the viral ... Anaplasma phagocytophila), Q fever (Coxiella burnetii), Boutonneuse fever (Rickettsia conorii), ...
Additionally Spiroplasma bacteria are suspected symbionts, besides potentially the pathogens Anaplasma phagocytophilum, ...
Mi-Jin Lee; Joon-Seok Chae (2010). "Molecular detection of Ehrlichia chaffeensis and Anaplasma bovis in the salivary glands ... and Anaplasma bovis have been detected in H. longicornis. It has been associated with Russian spring-summer encephalitis, ...
2007). "Sp110 transcription is induced and required by Anaplasma phagocytophilum for infection of human promyelocytic cells". ...
It is also closely related to Wolbachia, Anaplasma, and Neorickettsia bacteria, with Rickettsia as a more distant genus. ... Lin, Mingqun; Rikihisa, Yasuko (2003-09-01). "Ehrlichia chaffeensis and Anaplasma phagocytophilum lack genes for lipid A ... unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions ...
It is a potential vector of many babesiosis pathogens like Babesia bigemina, Babesia bovis, and Anaplasma marginale. It shows a ...
The dominant parasites were Theileria species, T. buffeli, T. bicornis, Ehrlichia species, Anaplasma marginale and A. bovis. ...
Anaplasma marginale, and Baberia bigemina; 27 species of ixodid ticks have been found on waterbucks - a healthy waterbuck may ...
Six (see note below) species have been shown to cause human infection: Anaplasma phagocytophilum causes human granulocytic ... genera Ehrlichia and Anaplasma. These obligate intracellular bacteria infect and kill white blood cells. The average reported ...
Anaplasma centrale tends to infect the central region of red blood cells, and is sufficiently closely related to An. marginale ... Anaplasma marginale infects marginal areas of red blood cells of cattle and causes anaplasmosis wherever boophilid ticks occur ... Anaplasma phagocytophilum (formerly Ehrlichia phagocytophila) is a bacterium of deer that spreads to sheep where it causes tick ... However, some microbes, such as Anaplasma marginale and A. centrale, can also be transmitted by biting flies, or by blood on ...
Tabanid flies are also transmitters the bacteria Anaplasma marginale and A.centrale to cattle, sheep and goats, causing ... Scoles,G.A., (2008) Comparison of the efficiency of biological transmission of Anaplasma marginale (Rickettsiales: ...
D. variabilis may also carry Anaplasma phagocytophilum, the causative agent of human granulocytic anaplasmosis, and Ehrlichia ... and Anaplasma phagocytophilum in Ticks (Acari: Ixodidae) from a Coastal Region of California". J. Med. Entomol. 40 (4): 534-9. ...
2005). "Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer ...
Tick fever is caused by the diseases Babesia bigemina, Babesia bovis, or Anaplasma marginale which are transmitted by cattle ...
Anaplasmosis is a tickborne disease caused by the bacterium Anaplasma phagocytophilum. It was previously known as human ... Anaplasma coinfection with non-rickettsial pathogens has been reported in ,10% of patients. ...
Anaplasma marginale and Anaplasma centrale in cattle Anaplasma ovis and Anaplasma mesaeterum in sheep and goats Anaplasma ... Anaplasma is a genus of bacteria of the alphaproteobacterial order Rickettsiales, family Anaplasmataceae. Anaplasma species ... cite journal}}: Cite journal requires ,journal= (help) Anaplasma genomes in the JGI genome browser Anaplasma at the US National ... Anaplasma platys in dogs The Anaplasma sparouinense species is responsible for a rare zoonosis, the Sparouine anaplasmosis, ...
Definition and taxonomy of Anaplasma species with emphasis on morphologic and immunologic features. Zeitschrift Fur ... The organisms of the Anaplasma spp. are morphologically indistinguishable in stained blood films. The majority of A. centrale ...
Anaplasma sp identified:Prid:Pt:Bld:Nom:Modified Giemsa. Anaplasma sp identified:Prid:Pt:Tiss:Nom:Modified Giemsa ...
Anaplasma phagocytophilum. Anaplasma phagocytophilum infects a broader spectrum of hosts in comparison to E. canis, including ... Ehrlichia canis and E. chaffeensis infect monocytes, Anaplasma phagocytophilum and E. ewingii infect granulocytes, and A. ... Other Ehrlichia and Anaplasma Spp. That Infect Dogs. A. platys belongs to the A. phagocytophilum-genogroup. It infects ... Anaplasma phagocytophilum infection of dogs is usually an acute disease that does not tend to manifest as a chronic illness ...
Anaplasma phagocytophilum (=Ehrlichia equi, Ehrlichia phagocytophila, human granulocytic ehrlichiosis [HGE] agent) Anaplasma ... Anaplasma phagocytophilum (=Ehrlichia equi, Ehrlichia phagocytophila, human granulocytic ehrlichiosis [HGE] agent) Anaplasma ... of some Anaplasma and Ehrlichia spp., including E. canis and E. chaffeensis. Passage of Anaplasma or Ehrlichia spp. to tick ... of some Anaplasma and Ehrlichia spp., including E. canis and E. chaffeensis. Passage of Anaplasma or Ehrlichia spp. to tick ...
Dumler JS, Choi K, Garcia-Garcia J, Barat N, Scorpio D, Garyu J, Human granulocytic anaplasmosis and Anaplasma phagocytophilum. ... Multiplex real-time PCR for detection of Anaplasma phagocytophilum and Borrelia burgdorferi. J Clin Microbiol. 2004;42:3164-8. ... HGA is caused by a rickettsial bacterium, Anaplasma phagocytophilum (1), groups of which form dense aggregations in ... Mean reservoir competence of 14 host species (10 mammals and 4 birds) for Anaplasma phagocytophilum, southeastern New York, USA ...
Norway is tick-borne fever (TBF) caused by the bacteria Anaplasma phagocytophilum that is transmitted by the tick Ixodes ... Grøva, Lise; Olesen, Ingrid; Steinshamn, Håvard and Stuen , Snorre (2011) Prevalence of Anaplasma phagocytophilum infection and ...
title = "Human exposure to Anaplasma phagocytophilum in Portugal",. abstract = "A retrospective study to detect Anaplasma ... Human exposure to Anaplasma phagocytophilum in Portugal. / Santos, Ana Sofia; Bacellar, F.; Dumler, J. S. ... Santos, A. S., Bacellar, F., & Dumler, J. S. (2006). Human exposure to Anaplasma phagocytophilum in Portugal. In Century of ... Human exposure to Anaplasma phagocytophilum in Portugal. In Century of Rickettsiology; Emerging, Reemerging Rickettsioses, ...
This study concludes that Anaplasma phagocytophilum is the primary suspect responsible for the massive deaths observed in ... This study concludes that Anaplasma phagocytophilum is the primary suspect responsible for the massive deaths observed in ... Key words: Anaplasma phagocytophilum, ticks, zoonosis, cattle, Benin. ... Key words: Anaplasma phagocytophilum, ticks, zoonosis, cattle, Benin. ...
Fourteen vertebrate species (10 mammals and 4 birds) were assessed for their ability to transmit Anaplasma phagocytophilum, the ... Title : Reservoir Competence of Vertebrate Hosts for Anaplasma phagocytophilum Personal Author(s) : Keesing, Felicia;Hersh, ...
... Neitz, W.O.; Du Toit, P. ... Bovine anaplasmosis : the transmission of Anaplasma marginale to a black wildebeest (Conochaetes gnu). Login ...
Access Anaplasma Phagocytophilum Infection case definitions; uniform criteria used to define a disease for public health ...
Prevalence of Dirofilaria immitis, Ehrlichia canis, Borrelia burgdorferi sensu lato, Anaplasma spp. and Leishmania infantum in ... Prevalence of Dirofilaria immitis, Ehrlichia canis, Borrelia burgdorferi sensu lato, Anaplasma spp. and Leishmania infantum in ... Home » Prevalence of Dirofilaria immitis, Ehrlichia canis, Borrelia burgdorferi sensu lato, Anaplasma spp. and Leishmania ...
The Anaplasma phagocytophilum Antibodies (IgG, IgM) Quest lab test contains 1 test with 3 biomarkers. ... Anaplasma phagocytophilum Antibodies (IgG, IgM) The Anaplasma phagocytophilum Antibodies (IgG, IgM) test contains 1 test with 3 ... Anaplasma phagocytophilum Antibodies (IgG, IgM) - Anaplasma phagocytophilum is a tick-borne agent that causes an acute febrile ...
Infections of the central nervous system (CNS) can be divided into 2 broad categories: those primarily involving the meninges (meningitis; see the image below) and those primarily confined to the parenchyma (encephalitis).{file37574}Meningitis is a clinical syndrome characterized by inflammation of the meninges, the 3 layers of membranes that...
Anaplasma marginale, Trypanosoma vivax and Borrelia burgdorferi in cattle from the Northeastern region of Pará, Brazil. Sera ... Serological detection of Anaplasma marginale, Babesia bovis and Babesia bigemina in beef cattle of the northern and central- ... Serological detection of Anaplasma marginale, Babesia bovis and Babesia bigemina in beef cattle of the northern and central- ... The objective of this study was to assess the occurrence of antibodies against Anaplasma marginale, Babesia bovis and Babesia ...
Infections of the central nervous system (CNS) can be divided into 2 broad categories: those primarily involving the meninges (meningitis; see the image below) and those primarily confined to the parenchyma (encephalitis).{file37574}Meningitis is a clinical syndrome characterized by inflammation of the meninges, the 3 layers of membranes that...
... Cat.. No.. Name of ready to use. PCR Kit. Storage. DOC. Amount. Price*. ...
We PCR tested serum samples collected in Canada for Anaplasma serology and found 84.8%-95.8% sensitivity and 2.8 average cycle ... Serum can be acceptable for detecting Anaplasma spp. when whole blood is unavailable. ...
Anaplasma platys infection in Ixodes persulcatus ticks was 1.8%. For Dermacentor nuttalli ticks, Anaplasma phagocytophilum was ... An investigation of the presence of Anaplasma phagocytophilum as well as Anaplasma platys was conducted in a forest area of ... In this study, both Anaplasma phagocytophilum and Anaplasma platys infection were detected in ticks from the forest area of ... To the authors knowledge, this study is the first description of Anaplasma phagocytophilum and Anaplasma platys in ticks in ...
By ,August 8, 2022,Comments Off on Anaplasma phagocytophilum IFA ...
  • Anaplasma species are biologically transmitted by Ixodes deer-tick vectors, and the prototypical species, A. marginale, can be mechanically transmitted by biting flies and iatrogenically with blood-contaminated instruments. (wikipedia.org)
  • Species of veterinary interest include: Anaplasma marginale and Anaplasma centrale in cattle Anaplasma ovis and Anaplasma mesaeterum in sheep and goats Anaplasma phagocytophilum in dogs, cats, and horses (see human granulocytic anaplasmosis) Anaplasma platys in dogs The Anaplasma sparouinense species is responsible for a rare zoonosis, the Sparouine anaplasmosis, detected only in French Guiana, South America. (wikipedia.org)
  • The objective of this study was to determine, by indirect enzyme-linked immunosorbent assay (ELISA), the frequency of seropositive cattle to Babesia bigemina, B. bovis, Anaplasma marginale, Trypanosoma vivax and Borrelia burgdorferi in cattle from the Northeastern region of Pará, Brazil. (scite.ai)
  • The objective of this study was to assess the occurrence of antibodies against Anaplasma marginale, Babesia bovis and Babesia bigemina in beef cattle of the northern and central-western regions of Brazil. (scite.ai)
  • Key words: Anaplasmosis, Babesiosis, beef cattle, Northern Region, Central-Western Region ResumoO objetivo deste estudo foi verificar a ocorrência de anticorpos contra Anaplasma marginale, Babesia bovis e Babesia bigemina em bovinos de corte das regiões Norte e Centro Oeste do Brasil. (scite.ai)
  • Os resultados mostram que as regiões analisadas apresentam estabilidade enzoótica para a infecção por B. bovis e B. bigemina, não sendo observado o mesmo para Anaplasma marginale. (scite.ai)
  • To study the mechanism of Anaplasma marginale-tick cell interactions, we successfully developed an in vitro Dermacentor andersoni primary midgut cell culture system. (umn.edu)
  • Anaplasma marginale infection of midgut cells regulated the differential expression of tick α-(1,3)-fucosyltransferases A1 and A2. (umn.edu)
  • Se estudió el rol epidemiológico de la transmisión transplacentaria de Anaplasma marginale en bovinos. (gob.ar)
  • 0 Anaplasma marginale in the cattle of South Gujarat, India was detected in the PCR and LAMP by amplifying the fragment of msp5 gene. (jvbd.org)
  • in ruminants, with animals infected by at least four of six Anaplasma species (Anaplasma marginale, A. bovis, A. ovis, and A. phagocytophilum). (uniss.it)
  • The data presented in this paper demonstrate that Anaplasma marginale, a tick borne pathogen of cattle, can stably maintain a fluorescent marker during long-term persistent infection in the immunocompetent mammalian host and throughout a complete transmission cycle in the natural tick vector, Dermacentor andersoni. (usda.gov)
  • We tested the stability of transformed Anaplasma marginale during a complete in vivo infection cycle. (usda.gov)
  • It also is an experimental vector of Anaplasma marginale to cattle. (merckvetmanual.com)
  • Anaplasmosis is caused by the bacterium Anaplasma marginale. (dtnpf.com)
  • Anaplasmosis is a tick-borne disease caused by an obligate gram-negative bacteria, Anaplasma (A.) marginale. (lutzonilab.net)
  • The genus Anaplasma (Rickettsiales: Anaplasmataceae) comprises obligate intracellular Gram-negative bacteria that are mainly transmitted by ticks, and currently includes six species: Anaplasma bovis, Anaplasma centrale, Anaplasma marginale, Anaplasma phagocytophilum, Anaplasma platys, and Anaplasma ovis. (unl.pt)
  • A anaplasmose bovina é causada pela riquétsia intra-eritrocítica Anaplasma marginale, responsável por importantes prejuízos econômicos, por causa da alta morbidade e mortalidade em rebanhos bovinos suscetíveis. (embrapa.br)
  • 2021). Compounding the public health significance is the high rate of other tick-borne infections and co-infections, such as Babesia parasites, several species of Anaplasma, Ehrlichia, and Rickettsia bacteria, and several viruses. (hhs.gov)
  • Serum antibodies to Borrelia burgdorferi , Anaplasma phagocytophilum , and Babesia microti in recaptured white-footed mice. (cell.com)
  • Well, it turns out he's also got Anaplasma Phagocytophilum and Babesia in addition to the Ehrlichia Canis. (greytalk.com)
  • I don't know abou Anaplasma, but I know that Doxy doesn't work for Babesia. (greytalk.com)
  • PCR detected the highest number of dogs infected with Anaplasma platys, Babesia vogeli, and Erhlichia canis. (cliniciansbrief.com)
  • 2015) Relatively low prevalence of Babesia microti and Anaplasma phagocytophilum in Ixodes scapularis ticks collected in the Lehigh Valley region of eastern Pennsylvania. (muhlenberg.edu)
  • The pathogens identified in these ticks included bacteria of the genus Anaplasma (Anaplasma ovis, A. bovis, A. phagocytophilum) and piroplasmal protozoans (Theileria luwenshuni, Babesia motasi). (tubitak.gov.tr)
  • For my lab at SUNY Adirondack, State funding allowed for the establishment of a biorepository of clinical samples from across New York State which has been used repeatedly for research applications, notably for the identification of biomarkers that could be used for development of accurate diagnostic tests for Lyme disease and other tickborne coinfections, such as tickborne relapsing fever (TBRF), Babesia, Anaplasma, Bartonella. (nysenate.gov)
  • A few also tested positive for anaplasma and babesia. (92moose.fm)
  • others include Anaplasma, Babesia and Neoehrlichia. (sciencenorway.no)
  • Anaplasma phagocytophilum (9.2%), Babesia divergens (2.9%), Theileria sp. (gisaz.org)
  • Anaplasma species reside in host blood cells and lead to the disease anaplasmosis. (wikipedia.org)
  • Fourteen vertebrate species (10 mammals and 4 birds) were assessed for their ability to transmit Anaplasma phagocytophilum , the bacterium that causes human granulocytic anaplasmosis, to uninfected feeding ixodid ticks. (cdc.gov)
  • Anaplasma phagocytophilum, a bacterium transmitted by ticks, is responsible for granulocytic anaplasmosis, an emerging zoonosis that has never been reported nor identified previously in Benin and in the West African sub-region. (academicjournals.org)
  • Human granulocytic anaplasmosis (HGA) was first reported in the United States of America in 1994, 2 and since then Anaplasma phagocytophilum has been considered an emerging pathogen of public health importance. (who.int)
  • Ehrlichia chaffeensis (human monocytotropic ehrlichiosis), Anaplasma phagocytophilum (human granulocytotropic anaplasmosis), and other anaplasmataceae. (medlineplus.gov)
  • Human granulocytic anaplasmosis is a tickborne rickettsial infection of neutrophils caused by Anaplasma phagocytophilum . (cdc.gov)
  • Prof. Dumler himself discovered another Anaplasma bacterium that causes the disease human anaplasmosis 2 decades ago. (medicalnewstoday.com)
  • Dr. Jason A. Carlyon's talk focused on Anaplasma phagocytophilum, the agent of human granulocytic anaplasmosis (HGA). (lymediseaseassociation.org)
  • Their report of cases for 2020 enumerated 468 cases of non-Lyme tick-borne diseases including 385 cases of anaplasmosis, an infection caused by the bacterium Anaplasma phagocytophilum which can also be transmitted by the blacklegged deer tick (Ixodes scapularis) known to transmit the bacterium Borrelia burgdorferi commonly responsible for Lyme. (mofga.org)
  • A total of 54 wild rabbits captured from southeastern China were examined for Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato by polymerase chain reaction (PCR) assays. (elsevier.com)
  • Babesiosis, bartonellosis, Anaplasma, Powassan virus and other Borrelia microbes can make symptoms more severe and difficult to treat. (lymediseaseassociation.org)
  • Antibodies specific for Borrelia burgdorferi and Ehrlichia equi (Anaplasma phagocytophilum) were detected in the blood from three evaluated dogs. (slu.se)
  • Ehrlichia canis and E. chaffeensis infect monocytes, Anaplasma phagocytophilum and E. ewingii infect granulocytes, and A. platys infects platelets. (vin.com)
  • An investigation of the presence of Anaplasma phagocytophilum as well as Anaplasma platys was conducted in a forest area of Selenge province, Mongolia, where ticks are widely distributed and tick-borne diseases are highly endemic. (who.int)
  • infection of Anaplasma platys was detected in 1% of Ixodes persulcatus ticks and 10% of Dermacentor nuttalli ticks. (who.int)
  • 7 Primers designed to amplify the partial groEL gene encoding heat-shock protein of Anaplasma phagocytophilum EphplgroELF (5′-ATGGTATGCAGTTTGATCGC-3′) and EphplgroELR (5′-TCTACTCTGTCTTTGCGTTC-3′) were used and expected to yield a 625-bp product for Anaplasma phagocytophilum and for Anaplasma platys , respectively. (who.int)
  • Anaplasma phagocytophilum, and Rickettsia spp. (medscape.com)
  • Serological Evidence of Anaplasma phagocytophilum and Spotted Fever Group Rickettsia spp. (mdpi.com)
  • The genus Ehrlichia is closely related to the genera Rickettsia , Anaplasma , and Wolbachia . (kenyon.edu)
  • We report the first molecular detection of Anaplasma phagocytophilum, a novel Rickettsia-like and Ehrlichia-like species, in Rh. (eurekamag.com)
  • Increasing incidence of Ehrlichia chaffeensis and Anaplasma phagocytophilum in the United States, 2000-2007. (cdc.gov)
  • Ehrlichiosis (formerly called human monocytic ehrlichiosis) is most commonly caused by the bacterium Ehrlichia chaffeensis, and less commonly by Ehrlichia Anaplasma phagocytophilum (formerly E. phagocytophila), the Chills, malaise, nausea, vomiting, diarrhea, confusion, and conjunctival infections can also occur1,2. (fille-facile.fr)
  • Anaplasma phagocytophilum is a gram-negative obligate intracellular bacterium long recognized as a veterinary agent 1 and more recently as a human infection. (who.int)
  • Should your horse have another infection, it can be aggravated as a result of the infection by anaplasma. (wagwalking.com)
  • The tick salivary protein sialostatin L2 inhibits caspase-1-mediated inflammation during Anaplasma phagocytophilum infection. (umaryland.edu)
  • Early detection of Anaplasma phagocytophilum infection is important to improve success in treatment and the Accuplex assay was able to detect A. phagocytophilum antibodies as early as 14 days post-infection. (antechdiagnostics.com)
  • This research project will examine one mechanism by which these data might be explained - that infection with Bartonella, Ehrlichia, and/or Anaplasma species bacteria may predispose susceptible dogs to develop lymphoma. (smilingblueskies.com)
  • The purpose of this study is to search for evidence of Bartonella, Ehrlichia, and/or Anaplasma infection in Golden Retriever dogs with lymphoma, as compared to age- and sex-matched Golden Retrievers from the same geographic region. (smilingblueskies.com)
  • Ehrlichiosis is a tick-borne bacterial infection, caused by bacteria of the family Anaplasmataceae, genera Ehrlichia and Anaplasma. (fille-facile.fr)
  • Anaplasma is also a bacterial infection, causing high fever, joint pain, vomiting and diarrhea and can be treated successfully if caught early. (scottveterinaryclinic.vet)
  • A retrospective study to detect Anaplasma phagocytophilum antibodies by indirect immunofluorescence (IFA) and Western blot (WB) assay was conducted in 367 potentially exposed patients from Portugal. (elsevier.com)
  • The Anaplasma phagocytophilum Antibodies (IgG, IgM) test contains 1 test with 3 biomarkers. (ultalabtests.com)
  • Anaplasma phagocytophilum Antibodies (IgG, IgM) - Anaplasma phagocytophilum is a tick-borne agent that causes an acute febrile illness that often resembles Rocky Mountain spotted fever. (ultalabtests.com)
  • Detection of antibodies to Anaplasma phagocytophilum (formerly known as Ehrlichia equi) by indirect fluorescent antibody test. (tamu.edu)
  • What is Anaplasma (Ehrlichiosis)? (wagwalking.com)
  • Equine granulocytic ehrlichiosis, is an infectious disease resulting from Anaplasma phagocytophilum bacteria that are transmitted by ticks from the Ixodes family (deer ticks). (wagwalking.com)
  • An infectious disease, anaplasma in horses, also known as equine granulocytic ehrlichiosis, is caused by the Anaplasma phagocytophilum bacteria being transmitted by ticks, leading to symptoms 10-20 days after the horse is infected. (wagwalking.com)
  • Its vector status is uncertain, but it has been implicated in the transmission of Ehrlichia and Anaplasma species, the causative agents of ehrlichiosis in humans and animals, and in Rocky Mountain spotted fever. (ufl.edu)
  • To the best of our knowledge, this is possibly the first report implicating pathogens of the genera Leishmania, Anaplasma and Hepatozoon as contributors in the etiopathogenesis of AML in the studied animal. (ufrgs.br)
  • Anaplasma in horses is caused by the bacteria Anaplasma phagocytophilum, which will infect horses through transmission by deer ticks. (wagwalking.com)
  • Ehrlichioses and anaplasmoses are infections caused by bacterial organisms of the genera Ehrlichia and Anaplasma that typically target certain host blood cells. (vin.com)
  • Ehrlichia and Anaplasma species are obligatory intracellular bacteria that are transmitted by ticks. (vin.com)
  • Ticks were examined for Ehrlichia and Anaplasma by nested PCR directed at the gltA, antigen-expressing gene containing a variable number of tandem repeats, 16S rRNA, and groESL genes. (cdc.gov)
  • Heartworm , Lyme disease , Ehrlichia and Anaplasma tests were normal, ruling out tick-borne disease as Chloe's culprit. (dogster.com)
  • Accuplex is a reference laboratory test used to screen for Lyme disease, heartworm, Ehrlichia, and Anaplasma. (antechdiagnostics.com)
  • When it comes to screening your patients for Heartworm, Lyme, Ehrlichia, and Anaplasma, Accuplex offers high-quality testing at an affordable price. (antechdiagnostics.com)
  • In this one hour webinar, you will learn more about Antech's Accuplex which is a reference laboratory test used to screen for Lyme disease, heartworm, Ehrlichia , and Anaplasma . (antechdiagnostics.com)
  • Anaplasma is a genus of bacteria of the alphaproteobacterial order Rickettsiales, family Anaplasmataceae. (wikipedia.org)
  • Few data are available on the prevalence and molecular typing of species belonging to the genus Anaplasma in Mediterranean ruminants. (uniss.it)
  • The presence of Anaplasma phagocytophilum, a tick-transmitted zoonotic pathogen, has been reported in Italy in humans and several animal species, including dogs, but data concerning its prevalence in the Italian canine population are lacking. (unipi.it)
  • Prevalence of serpositivity to spotted fever group rickettsiae and Anaplasma phagocytophilum in a large, dempgraphically diverse US sample. (medscape.com)
  • In this study, PCR analysis and sequencing of both 16S rRNA and groEL genes were combined to investigate the presence, prevalence, and molecular traits of Anaplasma spp. (uniss.it)
  • The results demonstrate a high prevalence of Anaplasma spp. (uniss.it)
  • In the UK the most common tick borne diseases in dogs are Lyme disease and Anaplasma. (yarrah.com)
  • This study concludes that Anaplasma phagocytophilum is the primary suspect responsible for the massive deaths observed in cattle in the study area. (academicjournals.org)
  • A tick bite injects Anaplasma storming defender neutrophils, Using MSP2 hooks to scale the walls. (cdc.gov)
  • Anaplasma phagocytophilum is a zoonotic agent of public health importance, infecting both humans and animals. (who.int)
  • We also show that the patient-derived groEL gene of Anaplasma (GenBank accession: OL770355 -OL770356) was closely related to the D-GB-gro-8-South Korea strain (5.5% of typhus suspected patients), and the rpoB gene of Leptospira (GenBank accession: OL770353-OL770354) was closely related to Linhai 56609-China strain (5.5% of typhus suspected patients). (org.np)
  • Anaplasma phagocytophilum is the widest spread tick-borne pathogen in animals in Europe and also severely affects the human health. (neuropatick.com)
  • The first study on human seroprevalence against Anaplasma phagocytophilum for central Asia reported a seroprevalence of 2.3% in Selenge province, 5.6% in Bulgan province, 2.8% in Dornogov province and 3.0% in both Tov province and Ulaanbaatar. (who.int)
  • Seroprevalence of anaplasma sp. (journalcra.com)
  • Molecular typing showed that Anaplasma sparouinense is distinct to all known species and more genetically related to recently described Anaplasma species causing infections in rainforest wild fauna of Brazil. (wikipedia.org)
  • The disease most commonly occurs in areas where competent tick vectors are indigenous, including tropical and semitropical areas of the world for intraerythrocytic Anaplasma spp. (wikipedia.org)
  • The objective of this study was to investigate the presence of Anaplasma phagocytophilumin tick vectors in a forest area of Selenge province, Mongolia. (who.int)
  • Anaplasma phagocytophilum, Coxiella burnetii, and possibly Rift Valley Fever virus. (blogspot.com)
  • Kohn, B. Vorkommen von Anaplasma phagocytophilum bei Blutspenderhunden in canine blood donors in Berlin/Brandenburg (2006-2012): retrospektive Auswertung klinischer Daten und Bedeutung für die Transfusionsmedizin. (fu-berlin.de)
  • Serological and molecular survey of Anaplasma phagocytophilum in Italian hunting dogs. (unipi.it)
  • In Mongolia, livestock play an important role as reservoirs of Anaplasma phagocytophilum in endemic areas. (who.int)