A genus of gram-negative bacteria whose organisms are obligate parasites of vertebrates. Species are transmitted by arthropod vectors with the host range limited to ruminants. Anaplasma marginale is the most pathogenic species and is the causative agent of severe bovine anaplasmosis.
A species of gram-negative bacteria in the genus ANAPLASMA, family ANAPLASMATACEAE, formerly called Ehrlichia phagocytophila or Ehrlichia equi. This organism is tick-borne (IXODES) and causes disease in horses and sheep. In humans, it causes human granulocytic EHRLICHIOSIS.
A species of gram-negative bacteria and causative agent of severe bovine ANAPLASMOSIS. It is the most pathogenic of the ANAPLASMA species.
A disease of cattle caused by parasitization of the red blood cells by bacteria of the genus ANAPLASMA.
A tick-borne disease characterized by FEVER; HEADACHE; myalgias; ANOREXIA; and occasionally RASH. It is caused by several bacterial species and can produce disease in DOGS; CATTLE; SHEEP; GOATS; HORSES; and humans. The primary species causing human disease are EHRLICHIA CHAFFEENSIS; ANAPLASMA PHAGOCYTOPHILUM; and Ehrlichia ewingii.
A species of gram-negative bacteria causing mild ANAPLASMOSIS in CATTLE. It also can infect SHEEP and GOATS. It is transmitted by TICKS.
A species of gram-negative bacteria producing mild to severe ANAPLASMOSIS in SHEEP and GOATS, and mild or inapparent infections in DEER and CATTLE.
The largest genus of TICKS in the family IXODIDAE, containing over 200 species. Many infest humans and other mammals and several are vectors of diseases such as LYME DISEASE, tick-borne encephalitis (ENCEPHALITIS, TICK-BORNE), and KYASANUR FOREST DISEASE.
Blood-sucking acarid parasites of the order Ixodida comprising two families: the softbacked ticks (ARGASIDAE) and hardbacked ticks (IXODIDAE). Ticks are larger than their relatives, the MITES. They penetrate the skin of their host by means of highly specialized, hooked mouth parts and feed on its blood. Ticks attack all groups of terrestrial vertebrates. In humans they are responsible for many TICK-BORNE DISEASES, including the transmission of ROCKY MOUNTAIN SPOTTED FEVER; TULAREMIA; BABESIOSIS; AFRICAN SWINE FEVER; and RELAPSING FEVER. (From Barnes, Invertebrate Zoology, 5th ed, pp543-44)
A widely distributed genus of TICKS, in the family IXODIDAE, including a number that infest humans and other mammals. Several are vectors of diseases such as TULAREMIA; ROCKY MOUNTAIN SPOTTED FEVER; COLORADO TICK FEVER; and ANAPLASMOSIS.
Bacterial, viral, or parasitic diseases transmitted to humans and animals by the bite of infected ticks. The families Ixodidae and Argasidae contain many bloodsucking species that are important pests of man and domestic birds and mammals and probably exceed all other arthropods in the number and variety of disease agents they transmit. Many of the tick-borne diseases are zoonotic.
Infections with bacteria of the family ANAPLASMATACEAE.
Infestations with soft-bodied (Argasidae) or hard-bodied (Ixodidae) ticks.
Members of the class Arachnida, especially SPIDERS; SCORPIONS; MITES; and TICKS; which transmit infective organisms from one host to another or from an inanimate reservoir to an animate host.
A family of bacteria which inhabit RED BLOOD CELLS and cause several animal diseases.
Small, often pleomorphic, coccoid to ellipsoidal organisms occurring intracytoplasmically in circulating LYMPHOCYTES. They are the etiologic agents of tick-borne diseases of humans; DOGS; CATTLE; SHEEP; GOATS; and HORSES.
A species of protozoa infecting humans via the intermediate tick vector IXODES scapularis. The other hosts are the mouse PEROMYSCUS leucopus and meadow vole MICROTUS pennsylvanicus, which are fed on by the tick. Other primates can be experimentally infected with Babesia microti.
Diseases of domestic cattle of the genus Bos. It includes diseases of cows, yaks, and zebus.
Proteins isolated from the outer membrane of Gram-negative bacteria.
The family Cervidae of 17 genera and 45 species occurring nearly throughout North America, South America, and Eurasia, on most associated continental islands, and in northern Africa. Wild populations of deer have been established through introduction by people in Cuba, New Guinea, Australia, New Zealand, and other places where the family does not naturally occur. They are slim, long-legged and best characterized by the presence of antlers. Their habitat is forests, swamps, brush country, deserts, and arctic tundra. They are usually good swimmers; some migrate seasonally. (Walker's Mammals of the World, 5th ed, p1362)
Arthropods, other than insects and arachnids, which transmit infective organisms from one host to another or from an inanimate reservoir to an animate host.
A genus of tick-borne protozoan parasites that infests the red blood cells of mammals, including humans. There are many recognized species, and the distribution is world-wide.
The immature stage in the life cycle of those orders of insects characterized by gradual metamorphosis, in which the young resemble the imago in general form of body, including compound eyes and external wings; also the 8-legged stage of mites and ticks that follows the first moult.
A species of gram-negative bacteria that is the causative agent of human EHRLICHIOSIS. This organism was first discovered at Fort Chaffee, Arkansas, when blood samples from suspected human ehrlichiosis patients were studied.
A group of tick-borne diseases of mammals including ZOONOSES in humans. They are caused by protozoa of the genus BABESIA, which parasitize erythrocytes, producing hemolysis. In the U.S., the organism's natural host is mice and transmission is by the deer tick IXODES SCAPULARIS.
Animate or inanimate sources which normally harbor disease-causing organisms and thus serve as potential sources of disease outbreaks. Reservoirs are distinguished from vectors (DISEASE VECTORS) and carriers, which are agents of disease transmission rather than continuing sources of potential disease outbreaks.
A promyelocytic cell line derived from a patient with ACUTE PROMYELOCYTIC LEUKEMIA. HL-60 cells lack specific markers for LYMPHOID CELLS but express surface receptors for FC FRAGMENTS and COMPLEMENT SYSTEM PROTEINS. They also exhibit phagocytic activity and responsiveness to chemotactic stimuli. (From Hay et al., American Type Culture Collection, 7th ed, pp127-8)
Diseases of the domestic dog (Canis familiaris). This term does not include diseases of wild dogs, WOLVES; FOXES; and other Canidae for which the heading CARNIVORA is used.
A specific species of bacteria, part of the BORRELIA BURGDORFERI GROUP, whose common name is Lyme disease spirochete.
Genus of gram-negative, intracytoplasmic bacteria that are found primarily in VACUOLES of MONOCYTES in the BLOOD and MACROPHAGES of lymphoid or other tissues of DOGS; HORSES, and humans. (From Int. J. Syst. Evol. Microbiol. (2001) 51:2145-2165)
A family of hardbacked TICKS, in the subclass ACARI. Genera include DERMACENTOR and IXODES among others.
Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
A species of gram-negative bacteria in the family ANAPLASMATACEAE, that causes HEARTWATER DISEASE in ruminants.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Substances elaborated by bacteria that have antigenic activity.
Species of gram-negative bacteria in the family ANAPLASMATACEAE, causing EHRLICHIOSIS in DOGS. The most common vector is the brown dog tick. It can also cause disease in humans.
Diseases of domestic and mountain sheep of the genus Ovis.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Diseases of the domestic or wild goat of the genus Capra.
A genus of TICKS, in the family IXODIDAE, widespread in Africa. Members of the genus include many important vectors of animal and human pathogens.
Glands that secrete SALIVA in the MOUTH. There are three pairs of salivary glands (PAROTID GLAND; SUBLINGUAL GLAND; SUBMANDIBULAR GLAND).
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
A genus of gram-negative, aerobic, rod-shaped bacteria often surrounded by a protein microcapsular layer and slime layer. The natural cycle of its organisms generally involves a vertebrate and an invertebrate host. Species of the genus are the etiological agents of human diseases, such as typhus.
The relationships of groups of organisms as reflected by their genetic makeup.
EPIDEMIOLOGIC STUDIES based on the detection through serological testing of characteristic change in the serum level of specific ANTIBODIES. Latent subclinical infections and carrier states can thus be detected in addition to clinically overt cases.
Change in the surface ANTIGEN of a microorganism. There are two different types. One is a phenomenon, especially associated with INFLUENZA VIRUSES, where they undergo spontaneous variation both as slow antigenic drift and sudden emergence of new strains (antigenic shift). The second type is when certain PARASITES, especially trypanosomes, PLASMODIUM, and BORRELIA, survive the immune response of the host by changing the surface coat (antigen switching). (From Herbert et al., The Dictionary of Immunology, 4th ed)
An island of the West Indies. Its capital is St. George's. It was discovered in 1498 by Columbus who called it Concepcion. It was held at different times by the French and the British during the 18th century. The British suppressed a native uprising in 1795. It was an associate state of Great Britain 1967-74 but became an independent nation within the British Commonwealth in 1974. The original name referred to the Feast of the Immaculate Conception but it was later renamed for the Spanish kingdom of Granada. (From Webster's New Geographical Dictionary, 1988, p467 & Room, Brewer's Dictionary of Names, 1992, p219)
A genus of gram-negative, anaerobic, helical bacteria, various species of which produce RELAPSING FEVER in humans and other animals.
An infectious disease caused by a spirochete, BORRELIA BURGDORFERI, which is transmitted chiefly by Ixodes dammini (see IXODES) and pacificus ticks in the United States and Ixodes ricinis (see IXODES) in Europe. It is a disease with early and late cutaneous manifestations plus involvement of the nervous system, heart, eye, and joints in variable combinations. The disease was formerly known as Lyme arthritis and first discovered at Old Lyme, Connecticut.
A family of small, gram-negative organisms, often parasitic in humans and other animals, causing diseases that may be transmitted by invertebrate vectors.
Proteins found in any species of bacterium.
Infections or infestations with parasitic organisms. The infestation may be experimental or veterinary.
Diseases of domestic and wild horses of the species Equus caballus.
Animals considered to be wild or feral or not adapted for domestic use. It does not include wild animals in zoos for which ANIMALS, ZOO is available.
Suspensions of attenuated or killed bacteria administered for the prevention or treatment of infectious bacterial disease.
Large, hoofed mammals of the family EQUIDAE. Horses are active day and night with most of the day spent seeking and consuming food. Feeding peaks occur in the early morning and late afternoon, and there are several daily periods of rest.
The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)
Any of numerous agile, hollow-horned RUMINANTS of the genus Capra, in the family Bovidae, closely related to the SHEEP.
A genus of tick-borne protozoa parasitic in the lymphocytes, erythrocytes, and endothelial cells of mammals. Its organisms multiply asexually and then invade erythrocytes, where they undergo no further reproduction until ingested by a transmitting tick.
Infection of cattle, sheep, or goats with protozoa of the genus THEILERIA. This infection results in an acute or chronic febrile condition.
Enzyme that catalyzes the first step of the tricarboxylic acid cycle (CITRIC ACID CYCLE). It catalyzes the reaction of oxaloacetate and acetyl CoA to form citrate and coenzyme A. This enzyme was formerly listed as EC 4.1.3.7.

Restriction of major surface protein 2 (MSP2) variants during tick transmission of the ehrlichia Anaplasma marginale. (1/149)

Anaplasma marginale is an ehrlichial pathogen of cattle that establishes lifelong persistent infection. Persistence is characterized by rickettsemic cycles in which new A. marginale variant types, defined by the sequence of the expressed msp2 transcripts, emerge. The polymorphic msp2 transcripts encode structurally distinct MSP2 proteins and result in an antigenically diverse and continually changing A. marginale population within the blood. In this manuscript, we used sequence analysis of msp2 transcripts to show that a restricted repertoire of variant types, designated SGV1 and SGV2, is expressed within the tick salivary gland. The same SGV1 and SGV2 variant types were expressed in ticks regardless of the variant types expressed in the blood of infected cattle at the time of acquisition feeding by the ticks. Importantly, subsequent tick transmission to susceptible cattle resulted in acute rickettsemia composed of organisms expressing only the same SGV1 and SGV2 variant types. This indicates that the msp2 expressed by organisms within the tick salivary gland predicts the variant type responsible for acute rickettsemia and disease. This restriction of transmitted A. marginale variant types, in contrast to the marked diversity within persistently infected cattle, supports development of MSP2 vaccines to prevent acute rickettsemia in tick-transmitted infections.  (+info)

Biased immunoglobulin G1 isotype responses induced in cattle with DNA expressing msp1a of Anaplasma marginale. (2/149)

Immunization with the native major surface protein 1 (MSP1) (a heterodimer containing disulfide and noncovalently bonded polypeptides designated MSP1a and MSP1b) of the erythrocytic stage of Anaplasma marginale conferred protection against homologous challenge (G. H. Palmer, A. F. Barbet, W. C. Davis, and T. C. McGuire, Science 231:1299-1302, 1986). The MSP1a polypeptide possesses a conserved neutralization-sensitive epitope. In the present study, the immune response to DNA-mediated immunization using msp1a was studied. The plasmid pVCL/MSP1a, which encodes the complete msp1a gene of A. marginale under the control of human cytomegalovirus immediate-early enhancer/promoter and intron A, was constructed. The immune responses elicited by immunization with pVCL/MSP1a into cardiotoxin-induced regenerating muscle were evaluated in mice and cattle. Antibody reactive with native MSP1a was detected in pooled sera of immunized BALB/c mice 3 weeks following primary immunization. Two calves seronegative for A. marginale were immunized four times, at weeks 0, 3, 7, and 13, with pVCL/MSP1a. By 8 weeks, both calves responded to MSP1a with an antibody titer of 1:100, which peaked at 1:1,600 and 1:800 by 16 weeks after the initial immunization. Interestingly, immunoblotting with anti-immunoglobulin G1 (anti-IgG1) and anti-IgG2 specific monoclonal antibodies revealed a restricted IgG1 anti-MSP1a response in both animals. T-lymphocyte lines, established after the fourth immunization, proliferated specifically against A. marginale homogenate and purified MSP1 in a dose-dependent manner. These data provide a basis for an immunization strategy to direct bovine immune responses by using DNA vaccine vectors containing single or multiple genes encoding major surface proteins of A. marginale.  (+info)

Emergence of Anaplasma marginale antigenic variants during persistent rickettsemia. (3/149)

Anaplasma marginale is an ehrlichial pathogen of cattle, in the order Rickettsiales, that establishes persistent cyclic rickettsemia in the infected host. Within each rickettsemic cycle, A. marginale expressing antigenically variant major surface protein 2 (MSP2) emerge. By cloning 17 full-length msp2 transcripts expressed during cyclic rickettsemia, we determined that emergent variants have a single, central hypervariable region encoding variant B-cell epitopes. The N- and C-terminal regions are highly conserved among the expressed A. marginale variants, and similar sequences define the MSP2 homologues in the agent of human granulocytic ehrlichiosis (HGE). This is in contrast to the MSP2 homologues in ehrlichial genogroup I pathogens, Ehrlichia chaffeensis, Ehrlichia canis, and Cowdria ruminantium, that have multiple hypervariable regions. By defining the variable and conserved regions, we were able to show that the single hypervariable region of A. marginale MSP2 encodes epitopes that are immunogenic and induce variant-specific antibody responses during persistent infection. These findings demonstrate that the MSP2 structural variants that emerge during each cycle of persistent rickettsemia are true antigenic variants, consistent with MSP2 antigenic variation as a mechanism of A. marginale persistence.  (+info)

Interleukin-12 as an adjuvant promotes immunoglobulin G and type 1 cytokine recall responses to major surface protein 2 of the ehrlichial pathogen Anaplasma marginale. (4/149)

Anaplasma marginale is a tick-transmitted pathogen of cattle closely related to the human ehrlichiae, Ehrlichia chaffeensis and the agent of human granulocytic ehrlichiosis (HGE). These pathogens have in common a structurally conserved outer membrane protein (OMP) designated the major surface protein 2 (MSP-2) in A. marginale and HGE and OMP-1 in E. chaffeensis. Protective immunity against ehrlichial pathogens is believed to require induction of gamma interferon (IFN-gamma) and opsonizing immunoglobulin (Ig) subclasses directed against OMP epitopes that, in concert, activate macrophages for phagocytosis and killing. Because interleukin-12 (IL-12) acts as an adjuvant for protein immunization to induce IFN-gamma and protective immunity against intracellular pathogens, we hypothesized that as an adjuvant with MSP-2, IL-12 would augment type 1 recall responses to A. marginale. IL-12 was coadsorbed with MSP-2 to alum and shown to significantly enhance IFN-gamma production by lymph node cells (LNC) and LNC-derived CD4(+) T-cell lines from immunized calves following recall stimulation with A. marginale. LNC proliferation and IL-2 production were also enhanced in IL-12-treated calves. Elevated recall proliferative responses by peripheral blood mononuclear cells were still evident 9 months after immunization. Serum IgG levels were consistently increased in IL-12 immunized calves, predominantly due to higher IgG1 responses. The results support the use of IL-12 coadsorbed with OMP of ehrlichial pathogens in alum to amplify both antibody and type-1 cytokine responses important for protective immunity.  (+info)

Expression of polymorphic msp1beta genes during acute anaplasma Marginale rickettsemia. (5/149)

Immunization of cattle with native MSP1 induces protection against Anaplasma marginale. The native immunogen is composed of a single MSP1a protein and multiple, undefined MSP1b polypeptides. In addition to the originally sequenced gene, designated msp1beta(F1), we identified three complete msp1beta genes in the Florida strain: msp1beta(F2), msp1beta(F3), and msp1beta(F4). Each of these polymorphic genes encodes a structurally unique MSP1b protein, and unique transcripts can be identified during acute A. marginale rickettsemia. The structural polymorphism is clustered in discrete variable regions, and each MSP1b protein results from a unique mosaic of five variable regions. Although each of the MSP1b proteins in the Florida strain contains epitopes recognized by serum antibody induced by protective immunization with the native MSP1 complex, the variable regions also include epitopes expressed by some but not all of the MSP1b proteins. These data support testing recombinant vaccines composed of the multiple antigenically and structurally unique MSP1b proteins combined with MSP1a in order to mimic the efficacy of native MSP1 immunization.  (+info)

Strain diversity in major surface protein 2 expression during tick transmission of Anaplasma marginale. (6/149)

Specific major surface protein 2 (MSP2) variants are expressed by Anaplasma marginale within the tick salivary gland and, following transmission, are expressed during acute rickettsemia. In previous work, we have shown that a restricted pattern of MSP2 variants is expressed in the salivary glands of Dermacentor andersoni ticks infected with the South Idaho strain of A. marginale. Now we demonstrate that the identical restriction does not apply to two other strains of A. marginale, and that different variants are also expressed when the same strain is transmitted by different Dermacentor spp. This indicates that antigenic diversity among strains is maintained in tick transmission and may be a significant constraint to MSP2 vaccine development.  (+info)

Antigenic variation in vector-borne pathogens. (7/149)

Several pathogens of humans and domestic animals depend on hematophagous arthropods to transmit them from one vertebrate reservoir host to another and maintain them in an environment. These pathogens use antigenic variation to prolong their circulation in the blood and thus increase the likelihood of transmission. By convergent evolution, bacterial and protozoal vector-borne pathogens have acquired similar genetic mechanisms for successful antigenic variation. Borrelia spp. and Anaplasma marginale (among bacteria) and African trypanosomes, Plasmodium falciparum, and Babesia bovis (among parasites) are examples of pathogens using these mechanisms. Antigenic variation poses a challenge in the development of vaccines against vector-borne pathogens.  (+info)

Antigenic variation of Anaplasma marginale by expression of MSP2 mosaics. (8/149)

Anaplasma marginale is a tick-borne pathogen, one of several closely related ehrlichial organisms that cause disease in animals and humans. These Ehrlichia species have complex life cycles that require, in addition to replication and development within the tick vector, evasion of the immune system in order to persist in the mammalian reservoir host. This complexity requires efficient use of the small ehrlichial genome. A. marginale and related ehrlichiae express immunoprotective, variable outer membrane proteins that have similar structures and are encoded by polymorphic multigene families. We show here that the major outer membrane protein of A. marginale, MSP2, is encoded on a polycistronic mRNA. The genomic expression site for this mRNA is polymorphic and encodes numerous amino acid sequence variants in bloodstream populations of A. marginale. A potential mechanism for persistence is segmental gene conversion of the expression site to link hypervariable msp2 sequences to the promoter and polycistron.  (+info)

Anaplasma spp. are tick-transmitted bacteria that infect a wide variety of wild and domestic animals. These pathogens exhibit a high degree of biological diversity, broad geographical distribution, and represent a serious threat to veterinary and public health worldwide. A novel Anaplasma species was identified in Haemaphysalis qinghaiensis (Ixodidae) in northwestern China and was molecularly characterized by comparison of 16S rRNA, gltA, and groEL gene sequences. Of the 414 samples tested, 24 (5.8%) were positive for this Anaplasma species. On the basis of the 16S rRNA gene, this organism has been found to be closely related to and exhibit the highest sequence similarity with A. capra (99.8-99.9%) that was identified in goats and humans in northern China, but was distinct from other known Anaplasma species. Sequence analysis of the gltA and groEL genes revealed that this Anaplasma species was distinct from A. capra considering the lower sequence identity (88.6-88.7% for gltA
The genus Anaplasma (Rickettsiales: Anaplasmataceae) comprises obligate intracellular Gram-negative bacteria that are mainly transmitted by ticks, and currently includes six species: Anaplasma bovis, Anaplasma centrale, Anaplasma marginale, Anaplasma phagocytophilum, Anaplasma platys, and Anaplasma ovis. These have long been known as etiological agents of veterinary diseases that affect domestic and wild animals worldwide. A zoonotic role has been recognized for A. phagocytophilum, but other species can also be pathogenic for humans.
Anaplasma species infection mimics many other conditions, including other chronic infections. If you are experiencing hard-to-diagnose chronic symptoms and have had a recent tick attachment or live in a region endemic for Anaplasma species in ticks, you should consider testing for Anaplasma infection. Symptoms can range from mild to serious illness characterized by fever, chills, headache, confusion, myalgia, nausea/vomiting/diarrhea, acute weight loss, abdominal pain, difficulty breathing, neurological problems and other conditions that may require immediate medical attention. This zoonotic infection is primarily transmitted to humans by Ixodes ticks, but can also be transmitted through blood transfusion. Clinical presentations may be more severe when co-infected with multiple tick-borne pathogens.. Tests Offered. Anaplasma species PCR test is designed to detect all pathogenic Anaplasma species. Positive results are confirmed by DNA sequencing to identify the infecting species. It is ...
Anaplasma spp. are widely spread rickettsial bacteria transmitted by ticks and placing high impacts on veterinary and public health. A limited number of studies have been carried out on Anaplasmosis in the central part of Iraq. This study was conducted to determine the presence of Anaplasma spp. in cattle in Al-Qadisiyah province, Iraq. A total of 400 blood specimens were collected from cattle suffering from heavy tick infestation. Cattle were blood-sampled from four hyper-endemic areas with ticks. Blood samples were screened using microscopic and polymerase chain reaction (PCR) methods. Diff-quick stained blood smears revealed Anaplasma-like inclusion bodies in 254 (63.5%) samples. According to the 16S rRNA-gene-based PCR analysis, Anaplasma spp. was detected in 124 of the 400 (31%) samples, divided as 96/254 (37.8%) among the microscopical positive samples and 28/146 (19.17%) among the microscopical negative samples. Phylogenetic analysis based on the partial 16S rRNA gene sequencing of ...
Shop Major surface protein ELISA Kit, Recombinant Protein and Major surface protein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Les Anaplasmataceae sont des bactéries intracellulaires transmises aux animaux et à lhomme, principalement par les tiques. Les Anaplasma spp. (A. marginale, A. phagocytophilum…) et les Ehrlichia spp. (E. canis, E. ruminantium...) en sont les principaux représentants dimportance économique et sanitaire. Les outils de diagnostic disponibles jouent un rôle déterminant dans leur identification. Nous proposons une approche globale par un système permettant didentifier la plupart des espèces dAnaplasmataceae. Il est basé sur le gène codant lARNr 23S ; il couple une qPCR et une PCR standard avec deux jeux damorces suivie dun séquençage. Il est testé pour en vérifier la spécificité, puis mis en oeuvre à partir de prélèvements de sang de ruminants et de tiques : dans les Pyrénées-Atlantiques, des brebis suspectes dinfection sont porteuses dA. ovis et leurs tiques Rhipicephalus bursa, dA. ovis, dA. phagocytophilum et dune nouvelle ehrlichia. En Haute-Corse, une forte ...
Clinical assay intended to detect the presence of Babesia and Anaplasma species in whole blood specimens.. If screening test is positive or equivocal, the test will automatically be referred for Lyme Disease Antibody, Confirmation, Serum testing at an additional charge ...
Classification of bacteria is challenging due to the lack of a theory-based framework. In addition, the adaptation of bacteria to ecological niches often results in selection of strains with diverse virulence, pathogenicity and transmission characteristics. Bacterial strain diversity presents challenges for taxonomic classification, which in turn impacts the ability to develop accurate diagnostics and effective vaccines. Over the past decade, the worldwide diversity of Anaplasma marginale, an economically important tick-borne pathogen of cattle, has become apparent. The extent of A. marginale strain diversity, formerly underappreciated, has contributed to the challenges of classification which, in turn, likely impacts the design and development of improved vaccines. Notably, the A. marginale surface protein 1a (MSP1a) is a model molecule for these studies because it serves as a marker for strain identity, is both an adhesin necessary for infection of cells and an immuno-reactive protein and is ...
Vector-borne pathogens are emerging concerns in multiple regions of Canada. Determining regional prevalence of canine vector-borne pathogens and documenting change will improve clinician awareness, enable targeted prevention, enhance diagnosis and ideally reduce the risk of disease. Study objectives were to: (i) estimate the prevalence of positive canine vector-borne test results from samples submitted in Canada; (ii) assess change in prevalence over time, from baseline (2008) to 2015; and (iii) estimate the prevalence of pathogen co-infections. This repeat cross-sectional study evaluated 753,468 test results for D. immitis antigen and B. burgdorferi, Ehrlichia canis/ewingii/muris serology, and 753,208 test results for Anaplasma phagocytophilum/platys serology using the SNAP® 4Dx®Test and SNAP 4Dx® Plus Test. Based on all submitted samples from Canada (2008-2015), the period seroprevalence of B. burgdorferi, Ehrlichia spp., Anaplasma spp. and D. immitis antigen were 2.0%, 0.5%, 0.4% and 0.2%,
Tick-borne pathogens are of great importance for small animal clinics and public health since they are increasingly prevalent among dogs and can infect humans (Jojima et al. 2008Jojima F.S., Garcia J.L., Vidotto M.C., Balarin M.R., Fabretti A.K., Gasparini M.R., Coelho A.L. & Vidotto O. 2008. Occurrence and molecular characterization of Babesia species in a canine hospital population in the Londrina Region, Parana State, Brazil. Revta Bras. Parasitol. Vet. 1:277-283., Maggi et al. 2013Maggi R.G., Mascarelli P.E., Havenga L.N., Naidoo V. & Breitschwerdt E.B. 2013. Co-infection with Anaplasma platys, Bartonella henselae and Candidatus Mycoplasma haematoparvum in a veterinarian. Parasit. Vectors 6:103.). In Brazil, there is lack of information about the distribution of hemoparasites and their vectors. This is the first study that evaluates the prevalence of Anaplasma platys, Ehrlichia canis and Babesia vogeli among dogs not domiciled in the southwestern region of Paraná, South of Brazil.. All ...
The establishment and maintenance of anaplasmosis-free cattle herds is impaired due to the lack of a rapid, sensitive, and specific serologic test to detect persistently infected cattle which serve as carriers for the organism. To develop an improved diagnostic test for anaplasmosis we screened Anaplasma marginale initial body proteins to identify a protein common to antigenically different isolates that is recognized by the host immune system at all stages of infection. Seronegative cattle were infected with either the Florida, Virginia, or North Texas isolate of A. marginale and monitored for infection by daily examination of Wright-stained blood smears for parasitized erythrocytes. Sera from cattle at different stages of infection, from acute through persistent, were used to immunoprecipitate A. marginale proteins that were metabolically radiolabeled with [35S]methionine or surface radiolabeled with 125I. Multiple A. marginale proteins were recognized by using sera either undiluted or at ...
A major surface protein complex from the Florida isolate of Anaplasma marginale has been previously shown to induce protection in immunized cattle and has been proposed as the basis of a subunit vaccine against anaplasmosis. This complex in the Florida isolate is composed of two noncovalently associated polypeptides with molecular masses of 105 and 100 kilodaltons (kDa). The analogous protein complex from four geographically different isolates of A. marginale was immunoprecipitated and compared with the protein complex of the Florida isolate. The polypeptides of the complex varied in apparent molecular mass among the isolates. By using antibodies recognizing epitopes on each polypeptide of the Florida isolate, the antigenic identity of the polypeptides in the analogous complexes was determined. The polypeptides recognized by the neutralizing monoclonal antibody 22B1, which recognizes a 105-kDa polypeptide in the Florida isolate, ranged from 70 to 100 kDa in the other isolates. Those polypeptides ...
TY - JOUR. T1 - Prevalence of human-Active and variant 1 strains of the tick-borne pathogen Anaplasma phagocytophilum in hosts and forests of Eastern North America. AU - Keesing, Felicia. AU - McHenry, Diana J.. AU - Hersh, Michelle. AU - Tibbetts, Michael. AU - Brunner, Jesse L.. AU - Killilea, Mary. AU - LoGiudice, Kathleen. AU - Schmidt, Kenneth A.. AU - Ostfeld, Richard S.. PY - 2014. Y1 - 2014. N2 - Anaplasmosis is an emerging infectious disease caused by infection with the bacterium Anaplasma phagocytophilum. In the eastern United States, A. phagocytophilum is transmitted to hosts through the bite of the blacklegged tick, Ixodes scapularis. We determined the realized reservoir competence of 14 species of common vertebrate hosts for ticks by establishing the probability that each species transmits two important strains of A. phagocytophilum (A. phagocytophilum human-Active, which causes human cases, and A. phagocytophilum variant 1, which does not) to feeding larval ticks. We also sampled ...
Shared and unique antigens were identified and partially characterized for two morphologically and antigenically distinct isolates of Anaplasma, A. marginale (Florida) (FAM) and A. caudatum (Illinois) (IAC). Crude CA-like antigens were prepared from the parasitemic blood of each calf, separated by SDS-PAGE, electroblotted onto nitrocellulose, and detected using immune bovine sera and an avidin-biotin-peroxidase assay with biotinylated rabbit anti-goat IgG (cross-reacted with cattle, sheep and deer antibodies). IAC antigens were 200 kilodalton (kd), 100 kd, 96 kd, 75 kd, 47 kd, 43 to 38 kd, and 27 kd, detected using anti-IAC and anti-FAM sera. FAM antigens were 108 kd, 100 kd, 96 kd, 91 kd, 75 kd, 47 kd, 43 to 38 kd, and 27 kd, detected using anti-FAM sera and, except for the 91 kd, with anti-IAC sera. The 91 kd is an FAM isolate-specific antigen. The 108 kd, 100 kd, and 96 kd are group-specific antigens of A. marginale and A. centrale. The 43 to 38 kd is genus-specific. The 96, 91, and 75 kd ...
The University of Missouri Veterinary Medical Diagnostic Laboratory (VMDL) is a full-service veterinary diagnostic laboratory and Missouris only laboratory accredited by the American Association of Veterinary Laboratory Diagnosticians. The VMDL is a Level I Lab of the National Animal Health Laboratory Network, Tier I Lab of the FDA Veterinary Laboratory Response Network, and a National Poultry Improvement Plan authorized lab. The VMDL maintains an effective quality assurance and quality control program. The VMDL has formal anatomical pathology, clinical pathology, and clinical microbiology training programs. The VMDL faculty hold academic appointments in the Departments of Veterinary Pathobiology and Veterinary Biomedical Sciences. The director is administratively responsible to the dean of the College of Veterinary Medicine for the overall operation of the laboratory and its multiple missions of public service, teaching, research and continuing education/extension. The VMDL faculty and staff ...
BACKGROUND: Only a few studies have examined the presence of Anaplasma marginale and Anaplasma centrale in South Africa, and no studies have comprehensively examined these species across the whole country. To undertake this country-wide study we adapted a duplex quantitative real-time PCR (qPCR) assay for use in South Africa but found that one of the genes on which the assay was based was variable ...
24.BraytonKA,PalmerGH,BrownWC: Genomicandproteomicapproaches tovaccinecandidateidentificationfor Anaplasmamarginale ExpertRev Vaccines 2006, 5: 95 101. 25.PierleSA,DarkMJ,DahmenD,PalmerGH,BraytonKA: Comparative genomicsandtranscriptomicsoftrait-geneassociation. BMCGenomics 2012, 13: 669. 26.VanOpijnenT,CamilliA: Transposoninsertionsequencing:anewtoolfor systems-levelanalysisofmicroorganisms. NatRevMicrobiol 2013, 11: 435 442. 27.ClaeysBouuaertC,ChalmersRM: Genetherapyvectors:theprospectsand potentialsofthecut-and-pastetransposons. Genetica 2010, 138: 473 484. 28.PicardeauM: Transpositionofflymarinerelementsintobacteriaasa genetictoolformutagenesis. Genetica 2010, 138: 551 558. 29.LampeDJ,GrantTE,RobertsonHM: Factorsaffectingtranspositionofthe Himar1 marinertransposoninvitro. Genetics 1998, 149: 179 187. 30.PlasterkRH,IzsvakZ,IvicsZ: Residentaliens:theTc1/marinersuperfamily oftransposableelements. TrendsGenet 1999, 15: 326 332. 31.ClarkTR,EllisonDW,KlebaB,HackstadtT: Complementationof Rickettsia ...
Ubiquinone (UQ), also called coenzyme Q, and plastoquinone (PQ) are electron carriers in oxidative phosphorylation and photosynthesis, respectively. The quinoid nucleus of ubiquinone is derived from the shikimate pathway; 4-hydroxybenzoate is directly formed from chorismate in bacteria, while it can be formed from either chorismate or tyrosine in yeast. The following biosynthesis of terpenoid moiety involves reactions of prenylation, decarboxylation, and three hydroxylations alternating with three methylations. The order of these reactions are somewhat different between bacteria and yeast. Phylloquinone (vitamin K1), menaquinone (vitamin K2), and tocopherol (vitamin E) are fat-soluble vitamins. Phylloquinone is a compound present in all photosynthetic plants serving as a cofactor for photosystem I-mediated electron transport. Menaquinone is an obligatory component of the electron-transfer pathway in bacteria ...
BLOT kit for the detection of specific IgG antibodies to the recombinant antigens of Anaplasma phagocytophilum (HGA) in human serum and plasma.
What is Anaplasmosis? Anaplasmosis is a disease caused by Anaplasma spp, a rickettsial parasite of ruminants, and is thus associated to rickettsial disease. Gram-negative microorganisms invade red blood cells and invade them. They are spread by a variety of haematophagous… Continue Reading → ...
Lineage: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales; Anaplasmataceae; Anaplasma; Anaplasma ...
Lineage: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales; Anaplasmataceae; Anaplasma; Anaplasma ...
A population of approximately 2500 dogs inhabits the island of Santa Cruz in the Galápagos Islands and many of these dogs freely roam the streets of the island in close proximity to people. The dog population receives minimal, if any, veterinary care or ectoparasite prophylaxis, leaving the majority of the dogs at risk for vector-borne infections such as Anaplasma platys, Borrelia burgdorferi, Dir ...
(34) Grouped under this heading are chlamydia, mycoplasma and other bacteria generally. Chlamydia These and anaplasma used to be classified separately from bacteria but are now...
I have a 20g with 3 platys and a BN. Recently I bought some live plants from Petco. They came with snails and within 3 days I had 2 dead female platys with what
2002) Erratum to A msp1α polymerase chain reaction assay for specific detection and differentiation of Anaplasma marginale isolates [Vet. Microbiol. 86 (2002) 325-335]. Veterinary Microbiology, 87 (4). pp. 365-366. ...
DETECTION OF Anaplasma marginale INFECTION IN A DAIRY CATTLE FARM BY STAINED BLOOD SMEAR EXAMINATION AND NESTED POLYMERASE CHAIN REACTION
Anaplasma marginale is the most prevalent tick-borne pathogen of cattle. It is transmitted by ixodid ticks. Symptoms of acute disease are anemia, weight loss, and often death. A.marginale establishes life-long persistent infection in animals that survive disease. These animals are clinically healthy but serve as reservoirs for transmission of the pathogen. Anaplasma marginale subsp. centrale (Anaplasma centrale (strain Israel)) is a naturally occurring attenuated strain has been used as a live vaccine to prevent severe disease due to A. marginale senso stricto strains for 100 years. A. marginale subsp. centrale contains 10 putative genes not found in the genomes of senso stricto strains, while 18 genes found in senso stricto strains are absent from A. marginale subsp. centrale. As inoculation with purified outer membrane protein (OMP) complexes provides immunity against A. marginale, it is important to identify OMPs that differ among A,marginale to identify further vaccine candidates. ...
TY - JOUR. T1 - Murine neutrophils require α 1,3-fucosylation but not PSGL-1 for productive infection with Anaplasma phagocytophilum. AU - Carlyon, Jason A.. AU - Akkoyunlu, Mustafa. AU - Xia, Lijun. AU - Yago, Tadayuki. AU - Wang, Tian. AU - Cummings, Richard D.. AU - McEver, Rodger P.. AU - Fikrig, Erol. PY - 2003/11/1. Y1 - 2003/11/1. N2 - Anaplasma phagocytophilum causes human granulocytic ehrlichiosis, the second most common tick-borne disease in the United States. Mice are natural reservoirs for this bacterium and man is an inadvertent host. A phagocytophilums tropism for human neutrophils is linked to neutrophil expression of P-selectin glycoprotein ligand-1 (PSGL-1), as well as sialylated and α1,3-fucosylated glycans. To determine whether A phagocytophilum uses similar molecular features to infect murine neutrophils, we assessed in vitro bacterial binding to neutrophils from and infection burden in wild-type mice; mice lacking α1,3-fucosyltransferases Fuc-TIV and Fuc-TVII; or mice ...
Clinical. Epidemiological elements + Clinical signs. Laboratory. Direct. Using light microscopy on peripheral stained blood smears, the diagnosis relies on the observation of a typical morula of Anaplasma platys in the platelets for thrombocytic anaplasmosis and the observation of a typical morula of Anaplasma phagocytophilum in the neutrophils for granulocytic anaplasmosis.. Infection is confirmed by PCR detection of bacterial DNA on tissues or blood.. Indirect. Indirect fluorescent antibody (IFA) assays to determine serum antibody against Anaplasma spp. available in veterinary laboratories.. Fast diagnosis at patient-side, based on an multivalent enzyme-linked immunosorbent assay (ELISA) is available for the detection of antibodies to E. canis, E. ewingii, A. phagocytophilum and A. platys.. Differential. Other tick-borne pathogens: Ehrlichia spp., Borrelia spp., Rickettsia spp.. Co-infection with other vector-borne pathogens is reported, especially co-infection with B. burgdorferi and A. ...
Anaplasma phagocytophilum is a zoonotic and obligate intracellular bacterium transmitted by ticks. In domestic ruminants, it is the causative agent of tick-borne fever, which causes significant economic losses in Europe. As A. phagocytophilum is difficult to isolate and cultivate, only nine genome sequences have been published to date, none of which originate from a bovine strain. Our goals were to; 1/ develop a sequencing methodology which efficiently circumvents the difficulties associated with A. phagocytophilum isolation and culture; 2/ describe the first genome of a bovine strain; and 3/ compare it with available genomes, in order to both explore key genomic features at the species level, and to identify candidate genes that could be specific to bovine strains. DNA was extracted from a bovine blood sample infected by A. phagocytophilum. Following a whole genome capture approach, A. phagocytophilum DNA was enriched 197-fold in the sample and then sequenced using Illumina technology. In total, 58.9%
Tick-borne pathogens cause emerging zoonoses, and include fastidious organisms such as Anaplasma phagocytophilum. Because of their obligate intracellular nature, methods for mutagenesis and transformation have not been available. To facilitate genetic manipulation, we transformed A. phagocytophilum (Ap) to express a green fluorescent protein (GFP) with the Himar1 transposase system and selection with the clinically irrelevant antibiotic spectinomycin. These transformed bacteria (GFP/Ap) grow at normal rates and are brightly fluorescent in human, monkey, and tick cell culture. Molecular characterization of the GFP/Ap genomic DNA confirmed transposition and the flanking genomic insertion locations were sequenced. Three mice inoculated with GFP/Ap by intraperitoneal injection became infected as demonstrated by the appearance of morulae in a peripheral blood neutrophil and re-isolation of the bacteria in culture.
Nango is neutered (April-12-2015), up to date with routine shots, micro-chipped, de-wormed and tested negative for heartworm.. Was likely abandoned as a puppy, when found collar outgrew causing neck laceration: Debridement and suture performed 04-13-2015 with full recovery. Blood Test January-25-2018. X-Rays January-25-2018. Patellar Luxation Examination January-25-2018. Dental Cleaning January-25-2018. Idexx SNAP® 4Dx® (April-11-2015). Tested negative for Heartworm, Lyme, Ehrlichia Canis, and Anaplasma Phagocytophilum. Polymerase Chain Reaction PCR Test (May-14-2015). Tested negative for Babesia spp. (B. Canis and B. Gibsoni), Ehrlichia spp. (E. Canis and Anaplasma Platys), Haemobartonella Canis, and Borrelia Burgdorferi. ...
Background: Tick-borne bacteria and protozoa can cause a variety of human and animal diseases in China. It is of great importance to monitor the prevalence and dynamic variation of these pathogens in ticks in ever-changing natural and social environment. Materials and Methods: Ticks were collected from Heilongjiang and Jilin provinces of northeastern China during 2018-2019 followed by morphological identification. The presence of Rickettsia spp., Anaplasma spp., Ehrlichia spp., Borrelia spp., Babesia spp., and Theileria spp. was examined by PCR and Sanger sequencing. The obtained sequences were subjected to phylogenetic analysis through Mega 7.0. Statistical analysis was performed using SPSS 24.0. Results: A total of 250 ticks from 5 species of 3 genera were collected. Ixodes and Haemaphysalis ticks carried more species of pathogens than Dermacentor, and the pathogens detected in Haemaphysalis japonica varied significantly among different sampling sites. The infection rates of Rickettsia spp., ...
Azad, AF., Radulovic, S., Higgins, JA., Noden, BH., Troyer, JM., 1997. Flea-borne rickettsioses: ecologic considerations. Emerg. Infect. Dis. 3, 319-327.. Beck, W., Boch, K., Mackensen, H., Wiegand, B. and Pfister, K., 2006 Qualitative and quantitative observations of the flea population dynamics of dogs and cats in several areas in Germany. Veterinary Pararsitology, 137, 130-136.. Bermann, F., Davoust, B., Fournier, P.E., Brisou-Lapointe, A.V., Brouqui, P., 2002. Ehrlichia equi (Anaplasma phagocytophila) infection in an adult horse in France. Vet. Rec., 150, 787-788.. Beugnet, F., Kenny, MJ., Day, MJ. , Shaw, SE. A PCR-based comparative survey of arthropod-transmitted infections in dogs, cats and prevalence in ticks in southern France. Proceedings, WAAVP 2003, New Orleans.. Beugnet, F., Loukos, H., Chalvet-Monfray, K., Bicout, D., 2008. FleaTickRisk : a climatic model developed to monitor and predict the activity and the density of 3 ticks species and the cat flea in France. Proceedings EMOP X, ...
Anaplasma marginale is a tick-transmitted, obligate intraerythrocytic pathogen of cattle, causing anaplasmosis characterized by anemia, decreased milk and meat production, and occasionally death. Animals that survive are ...
Ha, S., Furukawa, R., Stramiello, M., Wagner, J.J., and Fechheimer, M. (2011). Transgenic Mouse Model for the Formation of Hirano Bodies. BMC Neurosci. 12:97. [Epub ahead of print]. Ha, S., Furukawa, R., and Fechheimer, M. (2011). Association of AICD and Fe65 with Hirano bodies reduces transcriptional activation and initiation of apoptosis. Neurobiol Aging. 32:2287-98.. Kim, D.-H., R. Furukawa, and M. Fechheimer. 2009. Degradation of Hirano Bodies by Autophagy 5, 44-51.. Davis, R. C., Furukawa, R., and M. Fechheimer, 2008. A Mammalian Cell Culture Model for the Formation of Hirano Bodies, Acta Neuropathologica 115, 205-217.. Stich, R. W., G. A. Olah, K. A. Brayton, W. C. Brown, M. Fechheimer, K. Green-Church, S. Jittapalapong, K. M. Kocan, T. C. McGuire, F. R. Rurangirwa, and G. H. Palmer. 2004. Identification of a novel Anaplasma marginale appendage-associated protein that localizes with actin filaments during intraerythrocytic infection. Infection and Immunity 72, 7257-7264.. Maselli, A., R. ...
In der vorliegenden in vitro Studie sollte der Frage nachgegangen werden, inwieweit die Teilkronenpräparation Einfluss auf die marginale Integrität der vollkeramischen Restauration im Schmelz und im Dentin nach thermomechanischer Wechselbelastung hat. Es wurden zwei unterschiedliche Präparationsformen gewählt, wobei der vestibuläre, nichttragende Höcker auf 1,0 mm Restzahnhartsubstanzstärke ausgedünnt (Präparation A) oder zusätzlich um ca. 2,0 mm horizontal reduziert und überkuppelt (Präparation B) wurde. Von den Zähnen wurden vor und nach thermomechanischer Wechselbelastung Epoxidharzreplikate hergestellt, an denen die marginale Integrität computergestützt bei 201 facher Vergrößerung mittels rasterelektronenmikroskopischer Randanalyse sowohl an allen Grenzen (Schmelz/Befestigungskomposit, Dentin/Befestigungskomposit und Keramik/Befestigungskomposit), als auch an allen Flächen (okklusal/vestibulär, palatinal, approximal/mesial und approximal/distal) ermittelt wurde. Bei ...
It is defined as a disorder in which there is an infection caused by the bacteria. It resides in the cell and is known as the Anaplasma phago cyto philum.
I wanted to pass a little information to you all that cache with your pets, and also to make you aware of this as it can also affect humans. My dog was in serious joint pain and overall tenderness this week, lack of energy, and shivering at times. My first thought was lyme disease, as she has already experienced that quite a few years ago. Off to the vet we go, only to find out after blood work and testing in the office, that it is not lyme, but another tick issue called anaplasma. This is
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Prices - Initial Exam - $70.00 +Toe Nail Trim - $12.50Vaccine Exam - $70.00 +Microscopic Fecal Analysis - $48.004dx Test (hwt,lymes, tick fever, anaplasma) -
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I had thought to linger around the house in my Jammies and not do much, except possibly go buy some female platys because the lone female does not appreciate their attentions ...
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http://publications.ki.se/xmlui/discover?query=978-91-7409-752-8&submit=S%C3%B6k. 2011-10 Specialist in stomatognathic physioloogy (orofacial pain), Eastmaninstitutet Folktandvården AB. 2012-02 Doctoral Supervisor Training course, Karolinska Institutet. 2012-12 Teaching and learning in Higher Education, Karolinska Institutet. 2016-05 Specialist in Advanced Pain Medicine, Scandinavian Society of Anaesthesiology and Intensive Care Medicine (SSAI). 2017-01 Associate professor in odontology, Karolinska Institutet. ...
Serological surveys have confirmed Anaplasma marginale and Anaplasma phagocytophilum infections in dromedary camels, but molecular surveys and genetic characterisation of camel-associated Anaplasma species are lacking. In this study, we detected tick-borne Anaplasmataceae in 30 of 100 (30%) healthy dromedary camels screened using a combined 16S rRNA-groEL PCR-sequencing approach. Nucleotide sequencing confirmed Anaplasmataceae genome presence in 28 of the 33 16S rRNA PCR-positive samples, with two additional positive samples, for which 16S rRNA sequence data were ambiguous, being identified by groEL gene characterisation. Phylogenetic analyses of a 1289 nt segment of the 16S rRNA gene confirmed the presence of a unique Ehrlichia lineage and a discrete Anaplasma lineage, comprising three variants, occurring at an overall prevalence of 4% and 26%, respectively. Genetic characterisation of an aligned 559 nt groEL gene region revealed the camel-associated Anaplasma and Ehrlichia lineages to be novel ...
The genus Anaplasma consists of tick-transmitted obligate intracellular bacteria that invade white or red blood cells to cause debilitating and potentially fatal infections. A. phagocytophilum, a human and veterinary pathogen, infects neutrophils to cause granulocytic anaplasmosis. A. marginale invades bovine erythrocytes. Evidence suggests that both species may also infect endothelial cells in vivo. In mammalian and arthropod host cells, A. phagocytophilum and A. marginale reside in host cell derived pathogen-occupied vacuoles (POVs). While it was recently demonstrated that the A. phagocytophilum-occupied vacuole (ApV) intercepts membrane traffic from the trans-Golgi network, it is unclear if it or the A. marginale-occupied vacuole (AmV) interacts with other secretory organelles. Here, we demonstrate that the ApV and AmV extensively interact with the host endoplasmic reticulum (ER) in endothelial, myeloid, and/or tick cells. ER lumen markers, calreticulin and protein disulfide isomerase, and the ER
A TaqMan real-time PCR assay targeting the Anaplasma citrate synthase gene, gltA, was developed and used for detection of Anaplasma phagocytophilum in 765 Ixodes ricinus ticks collected from dogs and cats in northern Norway (n = 669) and Telemark county in southern Norway (n = 96). Among the ticks from northern Norway the prevalence of A. phagocytophilum was 3.0 %, while the prevalence in southern Norway was 2.1 % (p = 0.63). The gltA PCR assay showed a high analytical sensitivity (30 genomic units) and efficiency (98.5 %), and its utility in clinical diagnostics should be evaluated in future studies. This is the first report of A. phagocytophilum occurrence in ticks collected north of the Arctic Circle in Norway. The prevalence is comparable to that found in Telemark county in southern Norway.
Fourteen vertebrate species (10 mammals and 4 birds) were assessed for their ability to transmit Anaplasma phagocytophilum, the bacterium that causes human granulocytic anaplasmosis, to uninfected feeding ixodid ticks. Small mammals were most likely to infect ticks but all species assessed were capable of transmitting the bacterium, in contrast to previous findings.
Human granulocytic anaplasmosis (HGA) is an emerging tick-borne disease caused by Anaplasma phagocytophilum, an obligate intracellular bacterium of neutrophils....
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Drovers. Molecular biologist Susan Noh, at the Agricultural Research Services Animal Disease Research Unit in Pullman, Washington, is working to develop a vaccine to protect against anaplasmosis, a tick-transmitted disease of cattle. Caused by the microbe Anaplasma marginale, anaplasmosis affects cattle health, well-being, and production in many parts of the world and is characterized by severe anemia, fever, and weight loss. Despite this threat, there is no widely accepted vaccine for anaplasmosis.. Through their studies, Noh and her colleagues at Washington State University have identified important proteins to include in a potential vaccine, which is now being tested on animals. They found that small groups of the outer surface proteins of A. marginale induce an immune response that not only reduces symptoms, but can also prevent A. marginale infection in some animals. Some of the more promising vaccines being tested have protected 80 to 90 percent of the animals from clinical disease and ...
George Garrity, Don J. Brenner, James T. Staley, Noel R. Krieg, David R. Boone, Paul De Vos, Michael Goodfellow, Fred A. Rainey, Karl-Heinz Schleifer, ...,Bergeys Manual® of Systematic Bacteriology: Volume Two: The Proteobacteria, lk 123,2005,Googlei raamat veebiversioon (vaadatud 21.01.2014) (inglise keeles) ...
Cel mai adeasea, cei care locuiesc la bloc isi achizitioneaza si monteaza centrale termice cu condensare datorita faptului ca acestea consuma putina energie.. Nu trebuie sa mergi neaparat prin magazine si sa umbli zile in sir pentru a cumpara o centrala. Poti cumpara o centrala si online de pe Epinvest.ro. Intra pe site si consulta lista cu centralele disponibile.. Nu este convenabil sa cumperi centrale termice cu condensare atunci cand locuiesti la casa. Daca locuiesti la casa, cel mai convenabil este sa-ti achizitionezi o centrala termica pe lemne. Ai cu siguranta spatiu suficient pentru depozitarea lemnelor si suficient spatiu pentru camera in care tii centrala.. ...
Paul Salkin - Alors que les tensions russo-allemandes sont à leur comble en Europe, Cobourg et Hanovre, ethnologues de sang royal, débarquent au XXIe siècle dans un Congo qui entre dans lère des indépendances.
Baneth, G. (2010). Ehrlichia and anaplasma infections. Paper presented at World small animal veterinary congress. Retrieved ...
Anaplasma phagocytophilum causes granulocytic anaplasmosis. Borrelia burgdorferi causes Lyme disease. Rickettsia rickettsii ...
Anaplasma phagocytophilum Human metapneumovirus infection Human metapneumovirus (hMPV) Human monocytic ehrlichiosis Ehrlichia ...
December 2005). "Human granulocytic anaplasmosis and Anaplasma phagocytophilum". Emerging Infectious Diseases. 11 (12): 1828-34 ... and Anaplasma phagocytophilum in ticks (Acari: Ixodidae) from a coastal region of California". J. Med. Entomol. 40 (4): 534-9. ...
Many MSPs are found in Anaplasma and those which interact with Anaplasma can mainly be found in A. marginale and A. ... Anaplasma phagocytophilum shares its tick vector with other human pathogens, and about 10% of patients with HGA show serologic ... Anaplasma MSPs can not only cooperate with vertebrates, but also invertebrates, which make these phenotypes evolve faster than ... Lee HC, Kioi M, Han J, Puri RK, Goodman JL (September 2008). "Anaplasma phagocytophilum-induced gene expression in both human ...
136). Mackerras, I. M., Mackerras, M. J., & Mulhearn, C. R. (1942). Attempted transmission of Anaplasma marginale Theiler by ...
"mCherry - MCherry fluorescent protein - Anaplasma marginale - mCherry gene & protein". www.uniprot.org. Retrieved 2018-11-11. ...
Strains of Anaplasma bovis have also been found in rabbit ticks, which was discovered in a Nantucket tick study of the bacteria ... CS1 maint: discouraged parameter (link) Goethert, H.K.; S.R. Telford III (2003). "Enzootic Transmission of Anaplasma bovis in ...
21: Rickettsia, Orientia, Ehrlichia, Anaplasma, Coxiella and Bartonella". Microbiology and Immunology On-line. University of ...
T4SS has two effector proteins: firstly, ATS-1, which stands for Anaplasma translocated substrate 1, and secondly AnkA, which ... Rikihisa Y, Lin M, Niu H (September 2010). "Type IV secretion in the obligatory intracellular bacterium Anaplasma ...
... found worldwide Anaplasma centrale - found mainly in South America, Africa and the Middle East Sheep and goats: Anaplasma ovis ... Anaplasmosis is a disease caused by a rickettsial parasite of ruminants, Anaplasma spp and is therefore related to rickettsial ... It can be contracted from tick bites which contain Anaplasma phagocytophilum. The most common tick that carries the bacterium ... Several species of rickettsial bacteria cause anaplasmosis in ruminants: Cattle: Anaplasma marginale - ...
Anaplasma [24]. *Babesia [25] *Bartonella[26]. *Chlamydia trachomatis[27]. *Coxiella burnetii[28] ...
... and Anaplasma". Topics in Companion Animal Medicine. 26 (4): 173-7. doi:10.1053/j.tcam.2011.09.002. PMID 22152604. Callaway, ...
anaplasmosis Infection with Anaplasma, a genus of Sporozoa that infests red blood cells. anasa wilt A wilt disease of cucurbits ...
T4SS has two effector proteins: firstly, ATS-1, which stands for Anaplasma translocated substrate 1, and secondly AnkA, which ... "Type IV secretion in the obligatory intracellular bacterium Anaplasma phagocytophilum". Cellular Microbiology. 12 (9): 1213-21 ...
Like Anaplasma phagocytophilum, the causative agent of human granulocytic ehrlichiosis, Ehrlichia ewingii infects neutrophils. ...
The Anaplasmataceae are a Proteobacteria family that includes genera Anaplasma, Ehrlichia, Neorickettsia, and Wolbachia. " ...
... and its closely related genus Anaplasma show extreme diversity in the structure and content of their genomes. This ... "Structural basis for segmental gene conversion in generation of Anaplasma marginale outer membrane protein variants". Molecular ...
... and the bacterium Anaplasma marginale. Horses may be infected with Lyme disease, Anaplasma phagocytophila, and the viral ... Anaplasma phagocytophila), Q fever (Coxiella burnetii), Boutonneuse fever (Rickettsia conorii), ...
Mi-Jin Lee; Joon-Seok Chae (2010). "Molecular detection of Ehrlichia chaffeensis and Anaplasma bovis in the salivary glands ... Human diseases such as Lyme spirochetes, spotted fever group rickettsiae, Ehrlichia chaffeensis, and Anaplasma bovis have been ...
2007). "Sp110 transcription is induced and required by Anaplasma phagocytophilum for infection of human promyelocytic cells". ...
Hugenholtz P, Stackebrandt E (2004). "Reclassification of Sphaerobacter thermophilus from the subclass Sphaerobacteridae in the phylum Actinobacteria to the class Thermomicrobia (emended description) in the phylum Chloroflexi (emended description)". Int J Syst Evol Microbiol. 54 (Pt 6): 2049-51. doi:10.1099/ijs.0.03028-0. PMID 15545432 ...
It is also closely related to Wolbachia, Anaplasma, and Neorickettsia bacteria, with Rickettsia as a more distant genus. ... Lin, Mingqun; Rikihisa, Yasuko (2003-09-01). "Ehrlichia chaffeensis and Anaplasma phagocytophilum lack genes for lipid A ... unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions ...
It is a potential vector of many babesiosis pathogens like Babesia bigemina, Babesia bovis, and Anaplasma marginale. It shows a ...
The dominant parasites were Theileria species, T. buffeli, T. bicornis, Ehrlichia species, Anaplasma marginale and A. bovis. ...
Five (see note below) species have been shown to cause human infection: Anaplasma phagocytophilum causes human granulocytic ... genera Ehrlichia and Anaplasma. These obligate intracellular bacteria infect and kill white blood cells. The average reported ...
Anaplasma centrale tends to infect the central region of red blood cells, and is sufficiently closely related to An. marginale ... Anaplasma marginale infects marginal areas of red blood cells of cattle and causes anaplasmosis wherever boophilid ticks occur ... Anaplasma phagocytophilum (formerly Ehrlichia phagocytophila) is a bacterium of deer that spreads to sheep where it causes tick ... However, some microbes, such as Anaplasma marginale and A. centrale, can also be transmitted by biting flies, or by blood on ...
Tabanid flies are also transmitters the bacteria Anaplasma marginale and A.centrale to cattle, sheep and goats, causing ... Scoles,G.A., (2008) Comparison of the efficiency of biological transmission of Anaplasma marginale (Rickettsiales: ...
D. variabilis may also carry Anaplasma phagocytophilum, the causative agent of human granulocytic anaplasmosis, and Ehrlichia ... and Anaplasma phagocytophilum in Ticks (Acari: Ixodidae) from a Coastal Region of California". J. Med. Entomol. 40 (4): 534-9. ...
2005). "Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer ...
Anaplasmosis is a tickborne disease caused by the bacterium Anaplasma phagocytophilum. It was previously known as human ... Anaplasma coinfection with non-rickettsial pathogens has been reported in ,10% of patients. ...
Anaplasma phagocytophilum infection 2008. Current. Ehrlichia chaffeensis infection(https://wwwn.cdc.gov/nndss/conditions/ ... Ehrlichiosis and Anaplasmosis , 2008 Case Definition (https://wwwn.cdc.gov/nndss/conditions/anaplasma-phagocytophilum-infection ...
Anaplasma phagocytophilum (Ehrlichia phagocytophila)Imported. Automatic assertion inferred from database entriesi ... cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Anaplasma › ... tr,A0S0L6,A0S0L6_ANAPH AnkA OS=Anaplasma phagocytophilum OX=948 PE=4 SV=1 ...
Anaplasma marginale and Anaplasma centrale in cattle Anaplasma ovis and Anaplasma mesaeterum in sheep and goats Anaplasma ... Anaplasma genomes in the JGI genome browser Anaplasma at the US National Library of Medicine Medical Subject Headings (MeSH) ... Anaplasma is a genus of bacteria of the alphaproteobacterial order Rickettsiales, family Anaplasmataceae. Anaplasma species ... Anaplasma Genome Projects (from Genomes OnLine Database) Comparative Analysis of Anaplasma Genomes (at DOEs IMG system) v t e ...
Anaplasma phagocytophilum HZ Genome Page Anaplasma+phagocytophilum at the US National Library of Medicine Medical Subject ... Page Anaplasma on lpsn.dsmz.de Dumler JS, Barbet AF, Bekker CP, et al. (2001). "Reorganization of genera in the families ... Anaplasma phagocytophilum (formerly Ehrlichia phagocytophilum) is a Gram-negative bacterium that is unusual in its tropism to ... Thomas V, Fikrig E (July 2007). "Anaplasma phagocytophilum specifically induces tyrosine phosphorylation of ROCK1 during ...
Lyme disease is usually carried by the black-legged tick Ixodes scapularis. This duplex qPCR tests for both Borrelia burgdorferi & Anaplasma phagocytophilum. ...
Anaplasma phagocytophilum in central and western Wisconsin: a molecular survey.. Michalski M1, Rosenfield C, Erickson M, Selle ... Anaplasma phagocytophilum is an obligate intracellular bacterium that is transmitted to humans through the bite of Ixodes spp. ...
Background Anaplasma marginale, an obligate intracellular alphaproteobacterium in the order Rickettsiales, is a tick-borne ...
Anaplasma marginale str. Florida; Anaplasma marginale str. Illinois; Anaplasma marginale str. St. Maries; Anaplasma marginale ... Anaplasma marginale (Theiler, 1910) Description and significance. Anaplasma marginale is the most prevalent tick-borne, ... 5) NCBI Taxonomy Browser, "Anaplasma marginale" Retrieved 30 April, 2007 6) TGR-CMR, "Anaplasma marginale St. Maries Genome" ... "D425 Anaplasma Marginale" 8) Ueti MW, Reagan JO Jr, Knowles DP Jr, Scoles GA, Shkap V, Palmer GH., "Identification of midgut ...
Silencing expression of the defensin, varisin, in male Dermacentor variabilis by RNA interference results in reduced Anaplasma ... Experimental transmission of bovine anaplasmosis (caused by Anaplasma marginale) by means of Dermacentor variabilis and D. ... Silencing of genes involved in Anaplasma marginale-tick interactions affects the pathogen developmental cycle in Dermacentor ... Capillary tube feeding system for studying tick-pathogen interactions of Dermacentor variabilis (Acari: Ixodidae) and Anaplasma ...
Three isolates of Anaplasma marginale--Virginia (VAM), Illinois (IAM), and Florida (FAM)--were compared for infectivity for ... Infectivity of three Anaplasma marginale isolates for Dermacentor andersoni Am J Vet Res. 1987 Jan;48(1):96-9. ... Three isolates of Anaplasma marginale--Virginia (VAM), Illinois (IAM), and Florida (FAM)--were compared for infectivity for ...
Nieto NC, Foley JE, Bettaso J, Lane RS.,Reptile infection with Anaplasma phagocytophilum, the causative agent of granulocytic ... Selles artiklis on kasutatud saksakeelset artiklit de:Anaplasma phagocytophilum seisuga 22.01.2014. ... Varem on seda bakterit nimetatud mitmeti: Anaplasma phagocytophila, Ehrlichia phagocytophilum ja Ehrlichia phagocytophila, ka ... Anaplasma phagocytophilum muudab rakkudevahelist suhtlust selliselt, et suureneks IL-1 ja IL-8 tsütokiinide osakaal, ning see ...
Anaplasma phagocytophilum str. JM. Anaplasma phagocytophilum str. MRK. Anaplasma phagocytophilum str. NCH-1. Anaplasma ... Anaplasma phagocytophilum str. JM. Anaplasma phagocytophilum str. MRK. Anaplasma phagocytophilum str. NCH-1. Anaplasma ... Anaplasma phagocytophilum str. JM. Anaplasma phagocytophilum str. MRK. Anaplasma phagocytophilum str. NCH-1. Anaplasma ... Anaplasma phagocytophilum str. CRT53-1. Anaplasma phagocytophilum str. CRT38. Anaplasma phagocytophilum str. ApMUC09. 322. ...
The tick-transmitted Anaplasma phagocytophilumhas been recorded in a range of mammal species and causes granulocytic ... Kenny MJ, Parsons I, Shaw SE, Beugnet F (2003) Anaplasma (Ehrlichia) phagocytophilum infection in a UK fallow deer (Dama dama) ... Stuen S (2007) Anaplasma phagocytophilum-the most widespread tick-borne infection in animals in Europe. Vet Res Commun 31(Suppl ... Adamska M (2006) Detecting Anaplasma phagocytophilum DNA in blood of roe deer and in ticks. Med Weter 62:201-203 In Polish with ...
A total of 54 wild rabbits captured from southeastern China were examined for Anaplasma phagocytophilum and Borrelia ... Anaplasma phagocytophilum and Borrelia burgdorferi in rabbits from southeastern China.. *. [ Not Yet Rated ] [ Discuss This ... A total of 54 wild rabbits captured from southeastern China were examined for Anaplasma phagocytophilum and Borrelia ...
We have previously described a comparative genome analysis of nine strains of Anaplasma phagocytophilum that showed similarity ... Keywords: human anaplasmosis; Anaplasma phagocytophilum; comparative genomics; diagnosis; PCR human anaplasmosis; Anaplasma ... Comparative Genomics Identifies a Potential Marker of Human-Virulent Anaplasma phagocytophilum. Basima Al-Khedery 1. ... Al-Khedery, B.; Barbet, A.F. Comparative Genomics Identifies a Potential Marker of Human-Virulent Anaplasma phagocytophilum. ...
Anaplasma phagocytophilum,/i, causes great concern for livestock farmers. Tick-borne fever is a widespread disease in Norway, ... J. S. Dumler, "Anaplasma and Ehrlichia infection," Annals of the New York Academy of Sciences, vol. 1063, pp. 361-373, 2005. ... Anaplasma phagocytophilum, formerly Ehrlichia phagocytophila, is a vector-borne pathogen known to cause tick-borne fever (TBF) ... The complete A. phagocytophilum genome sequence has been assembled, and like that of other Anaplasma spp. and Ehrlichia spp., ...
Ubiquinone and other terpenoid-quinone biosynthesis - Anaplasma marginale Florida [ Pathway menu , Organism menu , Pathway ...
Browse by Exposure: Anaplasma (1 article). % of records by year: 1965 2017 ...
Sequence Analysis of the msp4 Gene of Anaplasma phagocytophilum Strains. José de la Fuente, Robert F. Massung, Susan J. Wong, ... Sequence Analysis of the msp4 Gene of Anaplasma phagocytophilum Strains. José de la Fuente, Robert F. Massung, Susan J. Wong, ... msp4 sequences for Anaplasma centrale (GenBank accession number AF428090), Anaplasma ovis (AF393742), and A. marginale (GenBank ... and Anaplasma spp. was done using the msp4 data for A. phagocytophilum, A. marginale, A. centrale, and A. ovis; the E. ...
Table 1. Anemia and thrombocytopenia associated with positive PCR forAnaplasma platys or serology forAnaplasma spp. from dogs. ... Cross-reaction evaluation of PCR-Anaplasma platys positive dogs tested to Anaplasma phagocytophilum antibodies by commercial ... Table 2. Anaplasma spp. serology and Anaplasma platys nPCR results in dogs. ... Positive Anaplasma sp. nPCR results with negative serological results might also indicate an early stage of infection.Anaplasma ...
Anaplasma marginale. The major surface protein 1a (MSP1a) has been used as a genetic marker for identifying A. marginale ...
The persistence of colostral Anaplasma marginale antibodies and incidence of in utero transmission of Anaplasma infections in ... Anaplasma marginale is a pathogen of the Rickettsiales order, family Anaplasmataceae (DUMLER et al., 2001), and is an obligate ... Anaplasma marginale (THEILER, 1910): Imunidade passiva e ativa em bovinos nascidos na primavera no sul do Rio Grande do Sul [ ... Anaplasma infections in wild and domestic ruminants: a review. J Wildl Dis 1984; 20(1): 12-20. PMid:6716555. [ Links ] ...
The investigation of Anaplasma phagocytophilum infection covered 111 red foxes from the Mazovian Province. Determination was ... Occurrence of Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti in Ixodes ricinus ticks collected ... Prevalence of Anaplasma phagocytophilum in Ixodes ricinus ticks determined by polymerase chain reaction with two pairs of ... High prevalence of Anaplasma phagocytophilum infection in ticks removed fromhuman skin in north-eastern Poland. ...
A novel zoonotic Anaplasma species is prevalent in small ruminants: potential public health implications. Download Prime PubMed ... Differential identification of Anaplasma in cattle and potential of cattle to serve as reservoirs of Anaplasma capra, an ... Molecular investigation of Anaplasma species in sheep from Heilongjiang Province, northeast China identified four Anaplasma ... AnaplasmaAnaplasmosisAnimalsChinaGoat DiseasesGoatsIxodesPhylogenyPublic HealthRuminantsSheepSheep DiseasesTick-Borne Diseases ...
We report the first isolation of Anaplasma phagocytophilum in South Korea. A 61-year-old woman presented with a 6-day history ... New genetic variants of Anaplasma phagocytophilum and Anaplasma bovis from Korean Water Deer (Hydropotes inermis argyropus). ... oa Clinical Isolation of Anaplasma phagocytophilum in South Korea * Seung Hun Lee1,†, Se Yoon Park2,5,†, Mi Jin Jang1, Ki Ju ... Anaplasma phagocytophilum, interferon gamma production and Stat1 signaling. Microbiol Immunol 57: 207-212.. [Google Scholar] ...
A genus of the family Anaplasmataceae; organisms form inclusions in red blood cells of ruminants Explanation of Anaplasma ... Looking for Anaplasma phagocytophilum? Find out information about Anaplasma phagocytophilum. ... Anaplasma. (redirected from Anaplasma phagocytophilum). Also found in: Medical, Wikipedia. Anaplasma. [‚an·ə′plaz·mə] ( ... equi and is now referred to as Anaplasma phagocytophilum (which will be used throughout the rest of the discussion).. Human ...
A genus of the family Anaplasmataceae; organisms form inclusions in red blood cells of ruminants Explanation of Anaplasma ... Looking for Anaplasma marginale? Find out information about Anaplasma marginale. ... Anaplasma. (redirected from Anaplasma marginale). Also found in: Medical. Anaplasma. [‚an·ə′plaz·mə] (microbiology) A genus of ... and Anaplasma marginale (among bacteria) and African trypanosomes, Plasmodium falciparum, and Babesia bovis (among parasites) ...
  • Anaplasma species reside in host blood cells and lead to the disease anaplasmosis. (wikipedia.org)
  • Species of veterinary interest include: Anaplasma marginale and Anaplasma centrale in cattle Anaplasma ovis and Anaplasma mesaeterum in sheep and goats Anaplasma phagocytophilum in dogs, cats, and horses (see human granulocytic anaplasmosis) Anaplasma platys in dogs The genomes from at least three different Anaplasma species have been sequenced. (wikipedia.org)
  • Reptile infection with Anaplasma phagocytophilum, the causative agent of granulocytic anaplasmosis. (wikipedia.org)
  • Anaplasma phagocytophilum, formerly Ehrlichia phagocytophila , is a vector-borne pathogen known to cause tick-borne fever (TBF) in ruminants and human granulocytic anaplasmosis (HGA) [ 1 ]. (hindawi.com)
  • Anaplasma phagocytophilum (Rickettsiales: Anaplasmataceae), also known as the human granulocytic ehrlichiosis (HGE) agent, Ehrlichia equi , and Ehrlichia phagocytophila , is the causative agent of human granulocytic anaplasmosis, tick-borne fever of ruminants, and equine and canine granulocytic anaplasmosis ( 16 ). (asm.org)
  • In this study, we have investigated the incidence of transplacental transmission of Anaplasma marginale in chronically infected cows with no history of acute anaplasmosis during gestation. (scielo.br)
  • Over the past two decades, Anaplasma phagocytophilum is considered to be mainly responsible for human anaplasmosis. (unboundmedicine.com)
  • scapularis collected in Indiana and examined during a recent study were found to harbor Babesia microti (the agent of human babesiosis) and, as far as is known, no human cases of babesiosis have been reported in Indiana, 5% of the ticks collected in Pulaski county were positive for Anaplasma phagocytophilum (the agent of human anaplasmosis) (Steiner et al. (thefreedictionary.com)
  • phagocytophilia Amblyoma Cowdriosis Cowdria ruminantium variegatum Anaplasmosis Anaplasma marginale R. (thefreedictionary.com)
  • hermsii Anaplasmosis Anaplasma marginale African trypanosomiasis African Trypanosoma spp. (thefreedictionary.com)
  • Bovine anaplasmosis or cattle-tick fever is a tick-borne haemolytic disease caused by the rickettsial haemoparasite Anaplasma marginale in tropical and subtropical areas of the world. (mdpi.com)
  • In the United States, Ixodes scapularis ticks overwinter in the Northeast and Upper Midwest and transmit the agent of human granulocytic anaplasmosis, Anaplasma phagocytophilum, among other pathogens. (jci.org)
  • The obligate intracellular pathogen, Anaplasma phagocytophilum , is the causative agent of human, equine, and canine granulocytic anaplasmosis and tick-borne fever (TBF) in ruminants. (frontiersin.org)
  • To develop an improved diagnostic test for anaplasmosis we screened Anaplasma marginale initial body proteins to identify a protein common to antigenically different isolates that is recognized by the host immune system at all stages of infection. (asm.org)
  • Both the presence of Anaplasma phagocytophilum in ticks and the seroprevalence of human granulocytic anaplasmosis have been reported in different parts of Europe. (aaem.pl)
  • Anaplasma phagocytophilum infection causes human granulocytic anaplasmosis (HGA), and the organism can infect neutrophils, neutrophil precursors, and endothelial cells ( 13 , 26 ). (asm.org)
  • Anaplasma phagocytophilum , a member of the family Anaplasmataceae and the obligate intracellular bacterium that causes granulocytic anaplasmosis, resides in a host cell-derived vacuole. (frontiersin.org)
  • Human granulocytic anaplasmosis (HGA) is an emerging tick-borne disease caused by Anaplasma phagocytophilum , an obligate intracellular bacterium that infects neutrophils and lacks pathways for both lipopolysaccharide (LPS) and peptidoglycan biosynthesis ( 10 , 16 ). (asm.org)
  • Anaplasma phagocytophilum, an intracellular rickettsial pathogen also transmitted by ixodid ticks, has emerged as an important zoonotic infection with significant veterinary and medical implications, and is responsible for both canine granulocytic anaplasmosis and human granulocytic anaplasmosis. (prohealth.com)
  • Anaplasma phagocytophilum is the tick-borne agent causing Human Granulocytic Anaplasmosis (HGA). (specialtylabs.com)
  • Differential gene transcription, especially of potential vaccine candidates, is of interest in Anaplasma marginale , the tick-borne causative agent of bovine anaplasmosis. (biomedcentral.com)
  • The etiologic agent of granulocytic anaplasmosis, Anaplasma phagocytophilum , has a circum-global distribution within the northern hemisphere and shows a host species predilection that varies by the geographic region in which the disease is found. (springer.com)
  • To address these challenges, the graph theory was applied to characterize the tick vector and human cell protein response to infection with Anaplasma phagocytophilum, the causative agent of human granulocytic anaplasmosis. (csic.es)
  • Human granulocytic anaplasmosis is caused by the obligate intracellular bacterium,Anaplasma phagocytophilum. (ovid.com)
  • Fourteen vertebrate species (10 mammals and 4 birds) were assessed for their ability to transmit Anaplasma phagocytophilum, the bacterium that causes human granulocytic anaplasmosis, to uninfected feeding ixodid ticks. (cdc.gov)
  • Ehrlichia chaffeensis primarily infects monocytes and causes human monocytic ehrlichiosis (HME) and Anaplasma phagocytophilum infects polymorphonuclear granulocytes and causes human granulocytic anaplasmosis (HGA). (mhmedical.com)
  • Human granulocytic anaplasmosis is a tickborne rickettsial infection of neutrophils caused by Anaplasma phagocytophilum. (semanticscholar.org)
  • Anaplasmosis has a global distribution and is caused by bacteria of the genus Anaplasma . (biomedcentral.com)
  • and 5) anaplasmosis, caused by Anaplasma phagocytophilum ( 2 ), also called human granulocytic anaplasmosis. (cdc.gov)
  • Anaplasma marginale major surface protein 1a: Human granulocytic anaplasmosis and Anaplasma phagocytophilum. (2019panamericangames.live)
  • Conclusions Anaplasma ovis was molecularly detected in goats and sheep from 12 provinces in China, with an overall infection rate of To date, over A. In the United States, anaplasmosis is notably present in the south and west, where the tick hosts Ixodes spp. (2019panamericangames.live)
  • Anaplasma bovis is a leukocytotropic agent of bovine anaplasmosis and there is no available information about molecular study on this agent in cattle of Iran. (iranjournals.ir)
  • The zoonotic disease human granulocytic anaplasmosis is a tick-borne rickettsial disease caused by the Gram-negative bacterium Anaplasma phagocytophilum. (surmodics.com)
  • Anaplasmosis is a disease caused by a rickettsial parasite of ruminants, Anaplasma spp. (wikimili.com)
  • Human granulocytic anaplasmosis ( HGA ) is a tick-borne, infectious disease caused by Anaplasma phagocytophilum , an obligate intracellular bacterium that is typically transmitted to humans by ticks of the Ixodes ricinus species complex, including Ixodes scapularis and Ixodes pacificus in North America. (wikimili.com)
  • Equine Granulocytic Anaplasmosis (EGA) is an acute tick-borne infection caused by Anaplasma phagocytophilum. (slub.se)
  • Human granulocytic anaplasmosis (HGA) is an emerging tick-borne disease caused by Anaplasma phagocytophilum, an obligate intracellular bacterium of neutrophils. (grantome.com)
  • Human granulocytic anaplasmosis (HGA) is a tick-borne disease caused by an intracellular bacterium, Anaplasma phagocytophilum that must live within neutrophils and similar cells. (grantome.com)
  • The new kit is based on the detection of antibodies against the bacteria of Anaplasma phagocytophila which causes the Human granulocytic anaplasmosis (HGA) disease. (testlinecd.com)
  • DOI 10.5326/JAAHA-MS-5578) Introduction dogs in 1996.5 Granulocytic anaplasmosis can cause hematologic Anaplasma phagocytophilum is a gram-negative intracellular bac- abnormalities, most typically thrombocytopenia, leukopenia, and terium transmitted by Ixodes species ticks and is the causative anemia.5-8 The mechanism of thrombocytopenia is not fully un- agent of granulocytic anaplasmosis.1 In the United States, Ixodes derstood. (spotidoc.com)
  • Anaplasma marginale is the causative agent of bovine anaplasmosis, a disease which can be protected by vaccination with the less pathogenic Anaplasma species, A. centrale. (edu.au)
  • A molecular test specific for A. marginale would be ideal for the identification of Anaplasma species in wild ruminants, as possible reservoirs of anaplasmosis, and to differentiate between A. marginale from A. centrale. (edu.au)
  • Anaplasma marginale is the most prevalent tick-borne, livestock pathogen worldwide and poses a considerable constraint to animal health. (kenyon.edu)
  • Silencing of genes involved in Anaplasma marginale -tick interactions affects the pathogen developmental cycle in Dermacentor variabilis . (nih.gov)
  • The tick-borne pathogen Anaplasma phagocytophilum causes great concern for livestock farmers. (hindawi.com)
  • However, a novel zoonotic pathogen provisionally named "Anaplasma capra" has recently been identified in China. (unboundmedicine.com)
  • Anaplasma capra is an emerging tick-transmitted zoonotic pathogen. (unboundmedicine.com)
  • These results also demonstrated pathogen- and tick-specific differences in the expression of HSPs and other SRPs in ticks and cultured tick cells infected with Anaplasma spp. (hindawi.com)
  • Genogroup II ehrlichia, including the agent of human granulocytic ehrlichiosis, Ehrlichia phagocytophila, and the bovine pathogen Anaplasma marginale , express a markedly immunodominant outer membrane protein designated major surface protein 2 (MSP2). (jimmunol.org)
  • Major surface protein 2 (MSP2) 3 is an immunodominant outer membrane protein (OMP) of the genogroup II ehrlichial pathogen Ehrlichia phagocytophila, the agent of human granulocytic ehrlichiosis (HGE), and Anaplasma marginale . (jimmunol.org)
  • Anaplasma platys, the causative agent of infectious canine cyclic thrombocytopenia, is a tick-borne pathogen that also has been implicated as potentially zoonotic. (veterinaryworld.org)
  • We are interested in exploring differences in pathogen transcription in these dissimilar environments and are using Anaplasma marginale as our model. (biomedcentral.com)
  • Over the past decade, the worldwide diversity of Anaplasma marginale, an economically important tick-borne pathogen of cattle, has become apparent. (csic.es)
  • Infection in cats: Anaplasma phagocytophilum is the most important feline pathogen among these rickettsial organisms, and coinfections are possible. (uzh.ch)
  • Functional genomic studies of tick cells in response to infection with the cattle pathogen, Anaplasma marginale. (semanticscholar.org)
  • article{Fuente2007FunctionalGS, title={Functional genomic studies of tick cells in response to infection with the cattle pathogen, Anaplasma marginale. (semanticscholar.org)
  • Anaplasma ovis is a widely distributed tick-borne rickettsial pathogen of sheep, goats, and wild ruminants. (2019panamericangames.live)
  • PCR confirmation of A. Indeed, a similar phenomenon was described with Anaplasma phagocytophilum experimental infection of a human megakaryocytic cell culture line, where platelet progenitors became infected by this pathogen, which is closely related anaplasmx A. (ccmz.info)
  • 9: Abstract Anaplasma platys is an obligate intracellular rickettsial pathogen that infects platelets of dogs, forming basophilic intracellular morulae. (tswatch03.me)
  • Anaplasma phagocytophilum ( Ap , formerly the Human Granulocytic Ehrlichiosis agent) is a common tick borne obligate intracellular pathogen with an uncommon tropism for host granulocytes. (biomedcentral.com)
  • Descriptions of this species of Anaplasma come from overseas, but the pathogen is also detectable in the Southern Mediterranean (North Africa, southern Portugal, Andalusia, Sicily, southern Italy, southern Greece). (laboklin.com)
  • Anaplasma species are biologically transmitted by Ixodes deer-tick vectors, and the prototypical species, A. marginale, can be mechanically transmitted by biting flies and iatrogenically with blood-contaminated instruments. (wikipedia.org)
  • Anaplasma the variation of P44 protein species in further confirmed the specific reaction phagocytophilum HL60 cells ( 2 , 5 ). (cdc.gov)
  • Survey of Ticks Collected from Tennessee Cattle and Their Pastures for Anaplasma and Ehrlichia Species. (nih.gov)
  • The tick-transmitted Anaplasma phagocytophilum has been recorded in a range of mammal species and causes granulocytic ehrlichiosis in humans, horses, and companion animals as well as tick-borne fever in ruminants. (springer.com)
  • For the characterization of genetic heterogeneity in this species, the homologue of Anaplasma marginale major surface protein 4 gene ( msp4 ) was identified, and the coding region was PCR amplified and sequenced from a variety of sources, including 50 samples from the United States, Germany, Poland, Norway, Italy, and Switzerland and 4 samples of A. phagocytophilum -like organisms obtained from white-tailed deer in the United States. (asm.org)
  • Phylogenetic analysis revealed that the isolates obtained from goats, sheep, Ixodes persulcatus ticks and humans create a separate clade within the genus Anaplasma and distinct from other recognized Anaplasma species. (unboundmedicine.com)
  • OVERVIEW: Anaplasma species, Ehrlichia species and Rickettsia species are vector-borne pathogens infecting a wide variety of mammals, but causing disease in very few of them. (uzh.ch)
  • In females of this tick species the prevalence rates of Anaplasma phagocytophilum and Borrelia burgdorferi s.l. were significantly lower in cemeteries, than in parks or forests. (uzh.ch)
  • Blood samples from four different states of Sudan were collected from apparently healthy cattle (n = 692), DNA was extracted and the prevalence of Babesia and Anaplasma species was analyzed by PCR. (unl.pt)
  • BACKGROUND : Only a few studies have examined the presence of Anaplasma marginale and Anaplasma centrale in South Africa, and no studies have comprehensively examined these species across the whole country. (up.ac.za)
  • A species of gram-negative bacteria in the genus ANAPLASMA, family ANAPLASMATACEAE, formerly called Ehrlichia phagocytophila or Ehrlichia equi. (semanticscholar.org)
  • Distinct host species correlate with Anaplasma phagocytophilum ankA gene clusters. (semanticscholar.org)
  • Whilst these techniques can confirm the presence of Anaplasma , they cannot discriminate between species as molecular methods can do. (biomedcentral.com)
  • Candidatus anaplasma camelii" in one-humped camels ( Camelus dromedarius ) in Morocco: a novel and emerging Anaplasma species? (biomedcentral.com)
  • Anaplasma species infection mimics many other conditions, including other chronic infections. (galaxydx.com)
  • If you are experiencing hard-to-diagnose chronic symptoms and have had a recent tick attachment or live in a region endemic for Anaplasma species in ticks, you should consider testing for Anaplasma infection. (galaxydx.com)
  • Anaplasma species PCR test is designed to detect all pathogenic Anaplasma species. (galaxydx.com)
  • unification of some species of Ehrlichia with Anaplasma , Cowdria with Ehrlichia and Ehrlichia with Neorickettsia , descriptions of six new species combinations and designation of Ehrlichia equi and 'HGE agent' as subjective synonyms of Ehrlichia phagocytophila . (dsmz.de)
  • Anaplasma species are intracellular bacteria that are within the family of Anaplasmataceae of the order Rickettsiales. (tswatch03.me)
  • Major surface protein Msp5 is conserved among the Anaplasma species and expressed in the salivary glands of infected ticks. (surmodics.com)
  • Based on genetic analyses, the former species Ehrlichia phagocytophila, Ehrlichia equi and the causative agent of human granulocytic ehrlichiosis (HGE) were unified in the new species Anaplasma phagocytophilum. (laboklin.com)
  • Currently, there is no polymerase chain reaction (PCR) assay available which differentiates between different species of Anaplasma or which can differentiate isolates of A. marginale within outbreaks and between different countries. (edu.au)
  • The genus Anaplasma which contains both animal and human pathogens. (kenyon.edu)
  • and Anaplasma marginale (among bacteria) and African trypanosomes, Plasmodium falciparum, and Babesia bovis (among parasites) are examples of pathogens using these mechanisms. (thefreedictionary.com)
  • In cattle infected with Anaplasma marginale , the number of pathogens in the blood varies between a peak of [10. (thefreedictionary.com)
  • Ticks are ectoparasites of animals and humans that serve as vectors of Anaplasma and other pathogens that affect humans and animals worldwide. (hindawi.com)
  • The obligatory intracellular pathogens Anaplasma phagocytophilum and Ehrlichia chaffeensis proliferate within membrane-bound vacuoles of human leukocytes and cause potentially fatal emerging infectious diseases. (springer.com)
  • Future studies on the functions of Anaplasma and Ehrlichia T4SS effector molecules and signaling pathways will undoubtedly advance our understanding of the complex interplay between obligatory intracellular pathogens and their hosts. (springer.com)
  • Is low-level persistent infection a common feature of pathogens in the genus Anaplasma? (2019panamericangames.live)
  • Anaplasma phagocytophilum is an obligate intracellular bacterium that is transmitted to humans through the bite of Ixodes spp. (nih.gov)
  • Bown KJ, Begon M, Bennett M, Woldehiwet Z, Ogden NH (2003) Seasonal dynamics of Anaplasma phagocytophila in a rodent-tick ( Ixodes trianguliceps ) system, United Kingdom. (springer.com)
  • Foley JE, Nieto NC, Adjemian J, Dabritz H, Brown RN (2008) Anaplasma phagocytophilum infection in small mammal hosts of Ixodes ticks, Western United States. (springer.com)
  • Anaplasma phagocytophilum (Rickettsiales: Anaplasmataceae) is an obligate intracellular bacterium mainly transmitted by Ixodes spp. (frontiersin.org)
  • Anaplasma phagocytophilum has for decades been known to cause the disease tick-borne fever (TBF) in domestic ruminants in Ixodes ricinus -infested areas in northern Europe. (biomedcentral.com)
  • Anaplasma phagocytophilum is transmitted by Ixodes scapularis , the same tick responsible for Lyme disease. (endocrinologyadvisor.com)
  • Role of migratory birds in introduction and range expansion of Ixodes scapularis ticks and of Borrelia burgdorferi and Anaplasma phagocytophilum in Canada. (semanticscholar.org)
  • Organism is now known as Anaplasma phagocytophilum transmitted by Ixodes scapularis (black-legged deer tick) on Eastern Seaboard, New England, Mid-Atlantic, and Upper Midwest, and on the West Coast by the western black-legged tick ( Ixodes pacificus )-the same vectors as for Lyme disease ( Borrelia burgdorferi ). (hopkinsguides.com)
  • Anaplasma phagocytophila , Ehrlichia phagocytophilum ja Ehrlichia phagocytophila , ka Ehrlichia egui . (wikipedia.org)
  • phagocytophila strains align into a clade that includes Anaplasma marginale , the historical precedent in this grouping. (thefreedictionary.com)
  • Prevalence of Anaplasma phagocytophilum, Rickettsia sp. (nih.gov)
  • Background: Tick-borne Anaplasma phagocytophilum and Rickettsia spp. (core.ac.uk)
  • Anaplasma marginale in bovine erythrocytes - "A Rickettsial agent transmitted by ticks. (kenyon.edu)
  • Adamska M (2006) Detecting Anaplasma phagocytophilum DNA in blood of roe deer and in ticks. (springer.com)
  • Once the detection techniques for Lyme disease were perfected, PHCR-W expanded its capabilities to test ticks and rodent tissue for other TBDs to include Ehrlichia chaffeensis, Anaplasma phagocytophilum , and Spotted Fever group Rickettsias. (thefreedictionary.com)
  • Expression of Heat Shock and Other Stress Response Proteins in Ticks and Cultured Tick Cells in Response to Anaplasma spp. (hindawi.com)
  • In this paper, the expression of heat shock proteins (HSPs) and other stress response proteins (SRPs) was characterized in ticks and cultured tick cells by proteomics and transcriptomics analyses in response to Anaplasma spp. (hindawi.com)
  • The results of these studies demonstrated that the stress response was activated in ticks and cultured tick cells after Anaplasma spp. (hindawi.com)
  • These results illustrated the complexity of the stress response in ticks and suggested a function for the HSPs and other SRPs during Anaplasma spp. (hindawi.com)
  • The aim of the study was to assess the prevalence of Anaplasma phagocytophilumin ticks, and to detect antibodies against the HGE agent in serum of forest workers in the region of Mid-Eastern Poland. (aaem.pl)
  • Ehrlichia and Anaplasma are both transmitted by ticks. (mhmedical.com)
  • Anaplasma phagocytophilum persists in nature by cycling between mammals and ticks. (semanticscholar.org)
  • Because ticks are such an issue in this area, we are recommending performing annual testing to screen for Lyme disease, heartworm, Anaplasma and Ehrlichia. (hillcrestanimalhospital.ca)
  • Trends in canine seroprevalence to Borrelia burgdorferi and Anaplasma spp. (medworm.com)
  • Comparative analysis of the infectivity rate of both Borrelia burgdorferi and Anaplasma phagocytophilum in humans and dogs in a New Jersey community. (prohealth.com)
  • Anaplasma is a genus of bacteria of the alphaproteobacterial order Rickettsiales, family Anaplasmataceae. (wikipedia.org)
  • Anaplasma marginale is a pathogenic gram-negative stain bacteria with an outer membrane composed of lipopolysaccharides. (kenyon.edu)
  • The causative agent of human granulocytic ehrlichiosis was recently reclassified as Anaplasma phagocytophilum , unifying previously described bacteria that cause disease in humans, horses, dogs, and ruminants. (asm.org)
  • We have previously described a comparative genome analysis of nine strains of Anaplasma phagocytophilum that showed similarity between strains infecting humans and U.S. dogs and a more distant relationship with horse and ruminant strains. (mdpi.com)
  • Al-Khedery B, Lundgren AM., Stuen S, Granquist EG, Munderloh UG, Nelson CM et al (2012) Structure of the type IV secretion system in different strains of Anaplasma phagocytophilum . (springer.com)
  • To assess these different hypotheses, we examined the effective population sizes of three strains of the bacterium Anaplasma phagocytophilum and categorized the varying roles of selection and demography on patterns of genetic diversity and divergence in these populations. (biomedcentral.com)
  • Table 2 Nucleotide sequence differences among groESL genes from different strains of Anaplasma platys. (tswatch03.me)
  • Phylogenetic relationship between 3 Chilean Anaplasma platys strains and other strains of the families Rickettsiaceae and Anaplasmataceae based on the groESL gene nucleotide sequences. (tswatch03.me)
  • Anaplasma centrale is a Gram-negative bacterium of the Rickettsiales order lacking a traditional cell wall. (kitpcr.com)
  • Serum samples from 196 dogs were first tested using dot-ELISA for antibodies against Anaplasma spp. (scielo.br)
  • Ten mouse monoclonal antibodies (MAbs) that react with Anaplasma phagocytophilum (the human granulocytic ehrlichiosis agent) Webster isolates were developed. (asm.org)
  • The Ehrlichia Immunofluorescence Assays (IFAs), Anaplasma phagocytophilum (HGA) IFA and Ehrlichia chaffeensis (HME) IFA are designed to detect human IgG and IgM antibodies to HGA and HME antigens, respectively, in human serum. (immdemo.com)
  • The Anaplasma phagocytophilum (HGA) IFA is used to detect antibodies to Anaplasma phagocytophilum in human serum. (immdemo.com)
  • BLOT kit for the detection of specific IgG antibodies to the recombinant antigens of Anaplasma phagocytophilum (HGA) in human serum and plasma. (testlinecd.com)
  • Mechanisms of obligatory intracellular infection with Anaplasma phagocytophilum. (semanticscholar.org)
  • Page Anaplasma on lpsn.dsmz.de Dumler JS, Barbet AF, Bekker CP, et al. (wikipedia.org)
  • Anaplasma platys - formerly known as Ehrlichia platys (DUMLER et al. (scielo.br)
  • According to Rule 27(2) b, Anaplasma platys is illegitimate because the derivation (etymology) of the specific epithet is provided neither in the paper by Dumler et al. (dsmz.de)
  • Three isolates of Anaplasma marginale--Virginia (VAM), Illinois (IAM), and Florida (FAM)--were compared for infectivity for Dermacentor andersoni. (nih.gov)
  • Casey ANJ, Birtles RJ, Radford AD, Bown KJ, French NP, Woldehiwet Z, Ogden NH (2004) Groupings of highly similar major surface protein (p44)-encoding paralogues: a potential index of genetic diversity amongst isolates of Anaplasma phagocytophilum . (springer.com)
  • Shared and unique antigens were identified and partially characterized for two morphologically and antigenically distinct isolates of Anaplasma, A. marginale (Florida) (FAM) and A. caudatum (Illinois) (IAC). (illinois.edu)
  • Anaplasma platys is a Gram-negative bacterium that belongs to the rickettsial family Anaplasmataceae. (ccmz.info)
  • Persistence of Anaplasma ovis infection and conservation of the msp-2 and msp-3 multigene families within the genus Anaplasma. (2019panamericangames.live)
  • Propagation of the Israeli vaccine strain of Anaplasma centrale in tick cell lines. (nih.gov)
  • Anaplasma centrale was prevalent in the Western Cape and KwaZulu-Natal and absent in the Northern Cape and Eastern Cape. (up.ac.za)
  • Domain combination pairs in Anaplasma centrale str. (bris.ac.uk)
  • VetPCR A.centrale Detection Kit is the direct detection of Anaplasma centrale on the basis of a genetic database, so it can diagnose very fast and accurately. (kitpcr.com)
  • Anaplasma phagocytophilum is a gram-negative, obligate intracellular bacterium, which particularly infects neutrophil granulocytes and forms, when multiplying within the granulocytes, typical inclusion bodies, so-called morulae. (laboklin.com)
  • Cyclic rickettsemia during persistent Anaplasma marginale infection of cattle. (thefreedictionary.com)
  • Submicroscopic levels of Anaplasma marginale rickettsemia in persistently infected cattle were determined by using nucleic acid hybridization. (asm.org)
  • Serum samples from a total of 707 goats, 433 cattle, and 219 dogs were collected for detecting Anaplasma phagocytophilum IgG antibody by immunofluorescence assays and the average seroprevalences were 10.05% for dogs, 3.82% for goats, and 0.69% for cattle, respectively. (ajtmh.org)
  • The results confirmed the presence of Babesia bigemina, Babesia bovis and Anaplasma marginale in cattle in northern Sudan with overall prevalence rates of 4.0%, 1.9% and 6.1%, respectively. (unl.pt)
  • The objective of this study was to estimate the accuracy of assays for detection of Anaplasma marginale infection in lactating dairy cattle of Puerto Rico using Bayesian methods without a perfect reference test. (up.ac.za)
  • Molecular survey of Anaplasma and Ehrlichia of red deer and sika deer in Gansu, China in Previous reports have reported that immunization of cattle with Msp1a induces partial protection when challenged with A. Conclusions Anaplasma ovis is widely distributed in the investigated geographical regions. (2019panamericangames.live)
  • Molecular Detection of Anaplasma bovis in Cattle from Central Part of Iran', Veterinary Research Forum , 1(2), pp. 117-122. (iranjournals.ir)
  • Detection of an Anaplasma marginale common surface protein present in all stages of infection. (asm.org)
  • Of six T4SS effector proteins identified in them, roles and functions have been described so far only for ankyrin repeat domain-containing protein A (AnkA), Anaplasma translocated substrate 1 (Ats-1), and Ehrlichia translocated factor 1 (Etf-1, ECH0825). (springer.com)
  • Anaplasma phagocytophilum (formerly Ehrlichia phagocytophilum) is a Gram-negative bacterium that is unusual in its tropism to neutrophils. (wikipedia.org)
  • Anaplasma phagocytophilum is a gram-negative bacterium formerly known as Ehrlichia phagocytophilum . (zoologix.com)
  • Differential expression of the tick protective antigen subolesin in anaplasma marginale - and A. phagocytophilum-infected host cells. (nih.gov)
  • Antigen used: combination of selected parts of the specific antigens of Anaplasma phagocytophilum (p44, Asp62, OmpA). (testlinecd.com)
  • A real-time combined polymerase chain reaction assay for the rapid detection and differentiation of Anaplasma phagocytophilum , Ehrlichia chaffeensis, and Ehrlichia ewingii. (thefreedictionary.com)
  • This assay uses human cells infected with Anaplasma phagocytophilum fixed on 12 well slides and incubated with diluted patient samples. (coppelabs.com)
  • The rapidSTRIPE Anaplasma Assay is a ready-to-use PCR kit for sensitive, highly specific detection of Anaplasma phagocytophilum. (environmental-expert.com)
  • Obligatory intracellular, human ehrlichiosis agents Ehrlichia chaffeensis and Anaplasma phagocytophilum have remarkable abilities to parasitize first-line immune defensive cells, monocytes/macrophages and granulocytes, respectively. (openthesis.org)
  • Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two super families of outer membrane proteins. (thefreedictionary.com)
  • Differential expression of genes in salivary glands of male Rhipicephalus (Boophilus)microplus in response to infection with Anaplasma marginale . (nih.gov)
  • Ehrlichia chaffeensis and Anaplasma phagocytophilum lack genes for lipid A biosynthesis and incorporate cholesterol for their survival. (semanticscholar.org)
  • In these studies, we used a functional genomics approach to characterize tick genes regulated in response to Anaplasma marginale infection. (semanticscholar.org)
  • The disease most commonly occurs in areas where competent tick vectors are indigenous, including tropical and semitropical areas of the world for intraerythrocytic Anaplasma spp. (wikipedia.org)
  • Here we describe the first successful transformation of Anaplasma phagocytophilum . (biomedcentral.com)
  • Ap-ha strain from the Ap-variant 1 strain of A. phagocy- he gram-negative bacterium Anaplasma phagocytophi- tophilum lum based on DNA sequence comparisons of the 16S is the causative agent of human granulocytic ana- rRNA gene of each strain (online Technical Appendix 1) plasmosis (HGA) in the United States ( 1 ). (cdc.gov)
  • In Venezuela, a few symptomatic human cases have been diagnosed since by the presence of platelet morulae in blood smears Ehrlichia platys Anaplasma platys in dogs from Maracaibo, Venezuela: In the present report, platts inclusions were documented in bone marrow thrombocyte precursors of two young naturally infected dogs, indicating anwplasma A. (tswatch03.me)
  • Call PuroClean of Chandler today and ensure minimal current and future human and business risk with regards to Anaplasma exposure with quick and discreet cleanup and sanitization services. (arizonarestorationcontractor.com)
  • This occurs when a case has compatible clinical criteria with laboratory evidence to support Ehrlichia/Anaplasma infection, but not with sufficient clarity to definitively place it in one of the categories previously described. (cdc.gov)
  • A clinical diagnosis of Ehrlichia (HME) or Anaplasma (HGA) may be difficult because symptoms can vary greatly from patient to patient. (immdemo.com)
  • Acute clinical, hematologic, serologic, and polymerase chain reaction findings in horses experimentally infected with a European strain of Anaplasma phagocytophilum. (slub.se)
  • Clinical signs of an Anaplasma phagocytophilum infection are typically seen within 1-2 weeks after the tick has bitten the dog and can include: lethargy, lack of appetite, and fever. (hillcrestanimalhospital.ca)
  • If a dog has been infected with Anaplasma platys, clinical signs can include lack of appetite, lethargy, fever, bruising on the gums and stomach, nosebleeds, and weight loss. (hillcrestanimalhospital.ca)
  • 47:6 Nov/Dec 2011 ª 2011 by American Animal Hospital Association Manifestations of Anaplasma phagocytophilum Infection exposure.11 Clinical illness usually occurs within 1-2 wk after tick NCSU). (spotidoc.com)
  • Anaplasma platys (formerly Ehrlichia platys) is an obligate intracellular, gram-negative bacterium in dogs which multiplies in thrombocytes and leads to cyclic thrombocytopenia and bacteraemia with intervals of approximately 14 days. (laboklin.com)
  • In ruminants, Anaplasma phagocytophilum can cause tick-bite fever. (laboklin.com)