Use of various chemical separation and extraction methods, such as SOLID PHASE EXTRACTION; CHROMATOGRAPHY; and SUPERCRITICAL FLUID EXTRACTION; to prepare samples for analytical measurement of components.
Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation.
Methodologies used for the isolation, identification, detection, and quantitation of chemical substances.
A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Food that has been prepared and stored in a way to prevent spoilage.
The art or practice of preparing food. It includes the preparation of special foods for diets in various diseases.
A diphenylmethane stimulant laxative used for the treatment of CONSTIPATION and for bowel evacuation. (From Martindale, The Extra Pharmacopoeia, 30th ed, p871)
Methods of preparing cells or tissues for examination and study of their origin, structure, function, or pathology. The methods include preservation, fixation, sectioning, staining, replica, or other technique to allow for viewing using a microscope.
Studies in which variables relating to an individual or group of individuals are assessed over a period of time.
The preparation, mixing, and assembling of a drug. (From Remington, The Science and Practice of Pharmacy, 19th ed, p1814)
Studies in which subsets of a defined population are identified. These groups may or may not be exposed to factors hypothesized to influence the probability of the occurrence of a particular disease or other outcome. Cohorts are defined populations which, as a whole, are followed in an attempt to determine distinguishing subgroup characteristics.
That portion of the electromagnetic spectrum from the UHF (ultrahigh frequency) radio waves and extending into the INFRARED RAYS frequencies.
Relating to the size of solids.
A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy.
A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection.
Studies in which the presence or absence of disease or other health-related variables are determined in each member of the study population or in a representative sample at one particular time. This contrasts with LONGITUDINAL STUDIES which are followed over a period of time.
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.
The field of medicine concerned with understanding the biochemical basis of health and disease and involved in developing diagnostic and therapeutic methods that utilize MOLECULAR BIOLOGY techniques.
The systematic identification and quantitation of all the metabolic products of a cell, tissue, organ, or organism under varying conditions. The METABOLOME of a cell or organism is a dynamic collection of metabolites which represent its net response to current conditions.
The dynamic collection of metabolites which represent a cell's or organism's net metabolic response to current conditions.
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
A plant genus of the family CUCURBITACEAE. It is a source of gypenosides and triterpenoid SAPONINS.
A discipline concerned with studying biological phenomena in terms of the chemical and physical interactions of molecules.
Mathematical procedure that transforms a number of possibly correlated variables into a smaller number of uncorrelated variables called principal components.
Techniques used to carry out clinical investigative procedures in the diagnosis and therapy of disease.
Control which is exerted by the more stable organizations of society, such as established institutions and the law. They are ordinarily embodied in definite codes, usually written.
Facilities equipped to carry out investigative procedures.
Laws and regulations, pertaining to the field of veterinary medicine, proposed for enactment or enacted by a legislative body.
A system for verifying and maintaining a desired level of quality in a product or process by careful planning, use of proper equipment, continued inspection, and corrective action as required. (Random House Unabridged Dictionary, 2d ed)
International organizations which provide health-related or other cooperative services.
The science of utilization, distribution, and consumption of services and materials.
A gamma-emitting radionuclide imaging agent used for the diagnosis of diseases in many tissues, particularly in the gastrointestinal system, liver, and spleen.
Two-phase systems in which one is uniformly dispersed in another as particles small enough so they cannot be filtered or will not settle out. The dispersing or continuous phase or medium envelops the particles of the discontinuous phase. All three states of matter can form colloids among each other.
The first artificially produced element and a radioactive fission product of URANIUM. Technetium has the atomic symbol Tc, atomic number 43, and atomic weight 98.91. All technetium isotopes are radioactive. Technetium 99m (m=metastable) which is the decay product of Molybdenum 99, has a half-life of about 6 hours and is used diagnostically as a radioactive imaging agent. Technetium 99 which is a decay product of technetium 99m, has a half-life of 210,000 years.
An element that is a member of the chalcogen family. It has an atomic symbol S, atomic number 16, and atomic weight [32.059; 32.076]. It is found in the amino acids cysteine and methionine.
A radiopharmaceutical used extensively in cholescintigraphy for the evaluation of hepatobiliary diseases. (From Int Jrnl Rad Appl Inst 1992;43(9):1061-4)
The production of an image obtained by cameras that detect the radioactive emissions of an injected radionuclide as it has distributed differentially throughout tissues in the body. The image obtained from a moving detector is called a scan, while the image obtained from a stationary camera device is called a scintiphotograph.
A metallic element, atomic number 49, atomic weight 114.82, symbol In. It is named from its blue line in the spectrum. (From Dorland, 28th ed)
Methods utilizing the principles of MICROFLUIDICS for sample handling, reagent mixing, and separation and detection of specific components in fluids.
The study of fluid channels and chambers of tiny dimensions of tens to hundreds of micrometers and volumes of nanoliters or picoliters. This is of interest in biological MICROCIRCULATION and used in MICROCHEMISTRY and INVESTIGATIVE TECHNIQUES.
Silicone polymers which consist of silicon atoms substituted with methyl groups and linked by oxygen atoms. They comprise a series of biocompatible materials used as liquids, gels or solids; as film for artificial membranes, gels for implants, and liquids for drug vehicles; and as antifoaming agents.
Exclusive legal rights or privileges applied to inventions, plants, etc.
Microdevices that combine microfluidics technology with electrical and/or mechanical functions for analyzing very small fluid volumes. They consist of microchannels etched into substrates made of silicon, glass, or polymer using processes similar to photolithography. The test fluids in the channels can then interact with different elements such as electrodes, photodetectors, chemical sensors, pumps, and valves.
Methods of creating machines and devices.
The preparation and analysis of samples on miniaturized devices.
A mass spectrometry technique used for analysis of nonvolatile compounds such as proteins and macromolecules. The technique involves preparing electrically charged droplets from analyte molecules dissolved in solvent. The electrically charged droplets enter a vacuum chamber where the solvent is evaporated. Evaporation of solvent reduces the droplet size, thereby increasing the coulombic repulsion within the droplet. As the charged droplets get smaller, the excess charge within them causes them to disintegrate and release analyte molecules. The volatilized analyte molecules are then analyzed by mass spectrometry.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
Chromatographic techniques in which the mobile phase is a liquid.
A commonly used laboratory solvent. It was previously used as an anesthetic, but was banned from use in the U.S. due to its suspected carcinogenicity.

Effects of sample dilution, peroxidase concentration, and chloride ion on the measurement of unbound bilirubin in premature newborns. (1/113)

OBJECTIVES: To assess the effects of sample dilution, peroxidase concentration, and chloride ion (Cl(-)) on plasma unbound bilirubin (B(f)) measurements made using a commercial peroxidase methodology (UB Analyzer) in a study population of ill, premature newborns. DESIGN AND METHODS: B(f) was measured with a UB Analyzer in 74 samples at the standard 42-fold sample dilution and compared with B(f) measured at a 2-fold sample dilution using a FloPro Analyzer. B(f) was measured at two peroxidase concentrations to determine whether the peroxidase steady state B(f) (B(fss)) measurements were significantly less than the equilibrium B(f) (B(feq)), in which case it was necessary to calculate B(feq) from the two B(fss) measurements. B(f) was also measured before and after adding 100 mmol/L Cl(-) to the UB Analyzer assay buffer. RESULTS: B(feq) at the 42-fold dilution was nearly 10-fold less than but it correlated significantly with B(feq) at the 2-fold dilution (mean 8.2+/-5.2 nmol/L versus 73.5+/-70 nmol/L, respectively, p<0.0001; correlation r=0.6). The two UB Analyzer B(fss) measurements were significantly less than B(feq) in 42 of 74 (57%) samples, and Cl(-) increased B(feq) in 66 of 74 (89%) samples by a mean of 82+/-67%. CONCLUSIONS: B(fss) measured by the UB Analyzer at the standard 42-fold sample dilution using assay buffer without Cl(-) and a single peroxidase concentration is significantly less than the B(feq) in undiluted plasma. Accurate B(f) measurements can be made only in minimally diluted serum or plasma.  (+info)

Isolation of polysome-bound mRNA from solid tissues amenable for RT-PCR and profiling experiments. (2/113)

Using cell lines and primary cells, it has been shown that translation control plays a key role regulating gene expression during physiological and pathological conditions. The relevance of this type of regulation in vivo (tissues, organs) remains to be elucidated, due to the lack of an efficient method for polysome-bound fractionation of solid tissue RNA samples. A simple and efficient method is described, in which tissue samples were pulverized in liquid nitrogen and lysed with NP40-lysis buffer in the presence of the RNAse inhibitors RNAsin and vanadyl-ribonucleoside complex. After cell lysis, the cytoplasmic extract was loaded into sucrose gradients, fractionated, and RNA prepared from each fraction. The obtained RNA was reverse transcribed with a low efficiency, a problem that was overcome by purifying polyA+ RNA. Aiming to use small quantities of solid tissue samples (10-20 mg/sample), polyA+ RNA purification was discarded, and the different components were individually screened for a negative effect on reverse transcription. The polysaccharide heparin, which is present as a nonspecific RNAse inhibitor, inhibits reverse transcriptase activity, and must be removed from RNA samples for an efficient reaction. Heparin was successfully removed by precipitation of the RNA with lithium chloride, as demonstrated by the reversal of the inhibition on RT-PCR reactions. In summary, we present a reliable method allowing us to prepare high-quality polysome-bound mRNA from small quantities of liquid-nitrogen-frozen solid tissue samples from both human and mouse origin, amenable for Northern blotting, RT-PCR reactions, and expression profiling analyses.  (+info)

Metabolism, pharmacokinetics, and excretion of a nonpeptidic substance P receptor antagonist, ezlopitant, in normal healthy male volunteers: characterization of polar metabolites by chemical derivatization with dansyl chloride. (3/113)

The excretion, biotransformation, and pharmacokinetics of ezlopitant [(2-benzhydryl-1-aza-bicyclo[2.2.2]oct-3-yl)-(5-isopropyl-2-methoxy-benzyl)-amine ], a substance P receptor antagonist, were investigated in healthy male volunteers after oral administration of a single 200-mg (approximately 93 microCi/subject) dose of [(14)C]ezlopitant. The total recovery of administered radioactive dose was 82.8 +/- 5.1, with 32.0 +/- 4.2% in the urine and 50.8 +/- 1.4% in the feces. Mean observed maximal serum concentrations for ezlopitant and total radioactivity were achieved at approximately 2 h after oral administration; thus, ezlopitant was rapidly absorbed. Ezlopitant was extensively metabolized in humans, since no unchanged drug was detected in urine and feces. The major pathway of ezlopitant in humans was the result of the oxidation of the isopropyl side chain to form the omega-hydroxy and omega-1-hydroxy (M16) metabolites. M16 and omega,omega-1-dihydroxy (1,2-dihydroxy, M12) were identified as the major circulating metabolites accounting for 64.6 and 15.4% of total circulating radioactivity, respectively. In feces, the major metabolite M14 was characterized as the propionic acid metabolite and formed by further oxidation of the omega-hydroxy metabolite. The urinary metabolites were the result of cleaved metabolites caused by oxidative dealkylation of the 2-benzhydryl-1-aza-bicyclo[2.2.2]oct-3-yl moiety. The metabolites (M1A, M1B, and M4), approximately 34% of the total radioactivity in urine, were identified as benzyl amine derivatives. These were polar metabolites that were further characterized using the reaction with dansyl chloride to derivatize the primary amines and phenol moieties to less polar analytes. The other metabolites were the result of O-demethylation, dehydrogenation of the isopropyl group, and oxidation on the quinuclidine moiety.  (+info)

Arsenic determination in marine sediment using ultrasound for sample preparation. (4/113)

This work deals with As determination in marine sediment using ultrasound for sample preparation. It is shown that As can be quantitatively extracted from marine sediment using 20% (v/v) HCl and sonication. The slurry is centrifuged and the analyte is determined in the supernatant by hydride generation atomic absorption spectrometry (HG AAS). A flow injection (FI) system is employed for hydride generation, with 0.5% (m/v) NaBH(4) used as reducdant and a 20% (v/v) HCl used as sample carrier. The limit of quantification is 1.6 microg g(-1) of As, which is based on 800 microl of sample solution and 0.200 g of sample mass in a volume of 50 mL. Certified and non certified marine sediment samples were analyzed; the results were in accordance with the certified or reference values. Speciation analysis by HPLC-ICP-MS showed that As(V) is the only detectable As species present in the supernatant of the centrifuged sample.  (+info)

On-plate digestion using a commercial microfraction collector for nano-HPLC matrix-assisted laser desorption/ionization tandem time-of-flight protein analysis. (5/113)

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Synthesis of bis(amino alcohol)oxalamides and their usage for the preconcentration of trace metals by cloud point extraction. (6/113)

C(2)-Symmetric two bis(amino alcohol)oxalamides (diamidediols) were synthesized and fully characterized. A new method was developed and successfully applied for the simultaneous preconcentration of both trace and toxic metals in water, by using C(2)-symmetric compounds. Under the optimum experimental conditions (i.e. pH = 10.0 +/- 0.2, 2.75 x 10(-3) mol L(-1) N,N'-bis[(1R)-1-ethyl-2-hydroxyethyl]ethanediamide (DAD1), 1.75 x 10(-3) mol L(-1) N,N'-bis[(1S)-1-benzyl-2-hydroxyethyl]-ethanediamide (DAD2), 0.10% w/v octylphenoxy-polyethoxyethanol (Triton X-114)), calibration graphs were linear in the range of 2.5 - 25.0 ng mL(-1) for Cu and Cd, 5.0 - 25.0 ng mL(-1) for Co and Ni. The enrichment factors were 18, 23, 18 and 20 for Cd, Cu, Co and Ni in the case of DAD1, respectively; 20, 22, 17 and 20 for Cd, Cu, Co and Ni in the case of DAD2. The limits of detection for DAD1 were found to be 0.45, 0.50, 1.25 and 0.60 ng mL(-1) for Cd, Cu, Co and Ni, respectively, and for DAD2 were found to be 0.44, 0.25, 0.60 and 1.55 ng mL(-1) for Cd, Cu, Co and Ni, respectively. The developed method was applied to the determination of Cu, Cd, Co and Ni in water samples and certified reference materials with satisfactory results.  (+info)

Triple-phase single-drop microextraction of silver and its determination using graphite-furnace atomic-absorption spectrometry. (7/113)

A new method is described for the determination of silver based on triple-phase microextraction using diethyldithio-carbamate (DDTC) and thioaminophenol. Ag is separated and preconcentrated from the matrix of the sample solution, and finally determined by electrothermal atomic-absorption spectroscopy. The parameters that affect the efficiency were investigated. Under the optimized conditions, a 30-fold preconcentration factor with a detection limit of 0.05 microg L(-1) was achieved. The relative standard deviation was 10% (5 determinations). The developed method was applied to the determination of trace Ag in water samples.  (+info)

Moving known libraries to an addressable array: a site-selective hetero-Michael reaction. (8/113)

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Sample preparation techniques for isolating contaminants using Light Microscopy, Electron Microscopy, FTIR, and SEM instrumentation are taught in this course.
This work concerns a new technique to post-modify the catalytic material intended for use in H2O2 direct synthesis. The catalyst chosen was a commercially available 1 wt.% Pd/C. The catalyst was modified with the so-called Catalyst Wet Pretreatment Method (CWPM) that is used to post-modify prepared catalysts with aqueous solutions of NaBr, in different concentrations. The performance of pristine and the pretreated materials were then compared in the H2O2 direct synthesis and characterized before and after the catalytic tests in order to understand the role of the different concentrations of bromide in the CWPM procedure. The surface features of the different catalysts were analyzed with CO chemisorption (metal dispersion and mean particle size), Transmission Electron Microscopy (TEM, for Pd morphology and Pd particle size distributions), Inductive Coupled Plasma (ICP, for Pd content) and Ion Chromatography (IC, for bromide content). Various features of the materials prepared with the CWPM were ...
Four new posters by Biotage R&D and Application Scientists have been presented this week at HPLC 2014, New Orleans, LA, USA. The studies focus on clean-up of biofluids using different sample preparation techniques, and how sample preparation is an important aspect of any analytical method. A number of different approaches are covered that can be used to remove the major endogenous components - particularly proteins and phospholipids - and may impact the quality of HPLC methods and data.. P77 Sample Preparation and Protein: A Comparison of Protein Removal with Various Sample Preparation Techniques using Gel ...
The present invention relates to methods for pretreating biological samples for extraction of nucleic acid therefrom. The present invention employs a combination of at least one protein denaturant with one or more of the following elements to form a reaction mixture for extraction of nucleic acid: (1) at least one aprotic solvent, (2) stepwise heating, and (3) sample dilution.
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
Read this case study and learn how the analysis of hair can be a useful tool in many forensic and clinical applications to establish drug use, trends of use and in the assessment of chronic alcohol consumption. Using the Genevac evaporation programme within these DoA produces, enables the excellent recovery of amphetamine, for example, which is renowned for its volatility.
There is a growing interest in monitoring microplastics in the environment, corresponding to increased public concerns regarding their potential adverse effects on ecosystems. Monitoring microplastics in the environment is difficult due to the complex matrices that can prevent reliable analysis if samples are not properly prepared first. Unfortunately, sample preparation methods are not yet standardized, and the various efforts to validate them overlook key aspects. The goal of this study was to develop a sample preparation method for wastewater samples, which removes natural organic matter without altering the properties of microplastics. Three protocols, based on KOH, H2O2, and Fenton reactions, were chosen out of ten protocols after a literature review and pre-experiments. In order to investigate the effects of these reagents on seven polymers (PS, PE, PET, PP, PA, PVC, and PLA), this study employed µFTIR, laser diffraction-based particle size analysis, as well as TD-Pyr-GC/MS. Furthermore, the
In the quest for renewable and sustainable energy, lignocellulosic biomass, such as herbaceous plants and hardwoods and softwoods, has been shown to be a promising feedstock for the production of second generation biofuels [1]. Lignocellulosic biomass essentially consists of the polysaccharides cellulose and hemicellulose and the aromatic macromolecule lignin. These compounds are present in the plant cell wall as a three-dimensional network giving the plant structure, stability, and resistance.. Pretreatment of the biomass is necessary in order to overcome this recalcitrance and facilitate degradation of polymeric structures [2]-[4]. In particular, the pretreatment methods aim to improve the conversion efficiency of the plant cell wall polysaccharides into fermentable monosaccharides by reducing the cellulose crystallinity or by simply splitting the carbohydrates and lignin for separate downstream processing technologies.. Various pretreatment methods have been developed for this purpose, ...
Article Characterization of Polymer Carbon Sieves, Graphitized Polymer Carbons and Graphitized Carbon Blacks for Sample Preparation Applications. The use of carbons in sample preparation techniques such as solid phase extraction (SPE) and air samplin...
Provide a fast and effective sample preparation technique for removal of phospholipids from biological matrices with Thermo Scientific HyperSep SLE (Solid supported Liquid/Liquid Extraction).HyperSep SLE plates (pH 9) deals with sample preparation of biological matrices via a simple, efficient and a
Provide a fast and effective sample preparation technique for removal of phospholipids from biological matrices with Thermo Scientific HyperSep SLE (Solid supported Liquid/Liquid Extraction).HyperSep SLE cartridges (pH 7) deals with sample preparation of biological matrices via a simple, efficient a
In order to analyze biological specimens for drugs such as benzodiazepines, it is necessary to perform sample preparation to eliminate matrix interference and ion suppression. Solid-phase extraction is generally the preferred sample preparation technique, in this study Disposable Pipette Extraction (DPX) was utilized. DPX is a fast dispersive solid-phase extraction technique that uses loosely contained sorbent in a disposable pipette tip. The sample is aspirated into the tip where it is actively mixed with the sorbent and forms a suspension. The main advantages of the DPX technology are that the extraction is rapid, minimal solvent waste is generated, and the entire process can be fully automated including introduction of the extract to the chromatographic system. The GERSTEL MPS autosampler performs DPX extractions in approximately 5 minutes using reversed phase (DPX-RP), cation exchange (DPX-CX) or immunoaffinity sorbent material. For determination of target drugs, GC/MS or HPLC/MS/MS are ...
Genotoxic and estrogenic activities in PET-bottled water have been reported. Chemical mixtures in bottled water have been suggested as the source of these toxicological effects. Furthermore, sample preparation techniques, such as solid-phase extraction (SPE), to extract estrogen-like compounds in bottled water are controversial. It has been suggested that inappropriate extraction methods and sample treatment may result in false-negative or positive responses when testing water extracts in bioassays. There is therefore a need to combine chemical analysis with bioassays to carry out hazard assessments. Formaldehyde, acetaldehyde and antimony are clearly related to migration from PET into water. However, several studies have shown other theoretically unexpected substances in bottled water. The origin of these compounds has not been clearly established (PET container, cap-sealing resins, background contamination, water processing steps, NIAS, recycled PET, etc.). [...] Our literature review shows ...
Hnasko, R.M., Lin, A.V., Stanker, L.H., Bala, K., McGarvey, J.A. 2016. Prion extraction methods: comparison of bead beating, ultrasonic disruption and repeated freeze-thaw methodologies for the recovery of functional renilla-prion fusion protein from bacteria. In: Micic, M., editor. Sample Preparation Techniques for Soil, Plant, and Animal Samples. New York, NY: Humana Press. p. 389-399 ...
With the growing use of illegal opioids, analysts should be prepared for a large influx of samples in their laboratories. While the workload may be increasing, the number of analysts and equipment may not, so the need for faster and better liquid chromatography-mass spectrometry (LC-MS) methods is important. By implementing an efficient sample preparation technique for matrix cleanup for some of the most common and traditional opioid matrices-blood and urine-coupled with a rapid and accurate LC method, laboratories can address the analytical needs for this growing problem. ...
The fusion method is an effective sample preparation technique for accurate analysis of a wide variety of powder samples including minerals and ores by XRF spectrometry since it eliminates heterogeneity due to grain size effects and mineralogical composition. In order to take advantage of the fusion method, however, appropriate corrections for errors arisen from fusion processes are required. In this application note the fusion method with improved correction for errors is demonstrated using cop
An improved system for screening a multiple of candidate therapeutic or chemotherapeutic agents for efficacy as to a specific patient, in which a tissue sample from the patient is harvested, cultured and separately exposed to a plurality of treatments and/or therapeutic agents for the purpose of objectively identifying. One particularly important tissue sample preparation technique is the initial preparation of cohesive multicellular particulates of the tissue sample. For assays concerning cancer treatment, a two-stage evaluation is contemplated in which both acute cytotoxic and longer term inhibitory effect of a given anti-cancer agent are investigated. The tissue sample technique of the present invention is also useful in assaying expression and/or secretion of various markers, factors or antigens present on or produced by the cultured cells for diagnostic purposes and for using such expression to monitor the applicability of certain candidate therapeutic or chemotherapeutic agents and the
Miyawakis team has been working on the project since 2011, when they discovered that an aqueous solution of urea was promising for brain tissue optical clearing, but they ran into the tissue damage problem. They have now refined the technique by using a solution which also contains the sugar alcohol sorbitol, as they explain in Nature Neuroscience.. By combining sorbitol in the right proportion with urea, we could create transparent brains with minimal tissue damage, that can handle both florescent and immunohistochemical labeling techniques, and is even effective in older animals, Miyawaki said.. The ScaleS solution also works as a preservative, keeping samples usable for over a year. It can also be combined with other sample preparation techniques: the team used it with a technique for labelling structures that trigger a response from the immune system and another for fluorescent chemical compounds to produce high-resolution images of Alzheimers plaques, which are not detectable in ...
It is the first course at the under graduate level on microstructural characterization of materials. This course will cover the basic principles and techniques of X-ray diffraction, optical, scanning electron and transmission electron microscopy along with demonstrations of the instrument details and imaging experiments through videos. This course also deals with the sample preparation techniques for the microstructural analysis with practical examples through videos. ...
Histofine® primary antibodies and ancillary reagents are developed by Nichirei Biosciences Inc. These primary antibodies designed to stain human tissue sections are compatible with FFPE tissue sections and used for histopathological analysis. Pretreatment methods and dilutions indicated in this catalog are recommended staining conditions using the Histofine® Simple Stain™ MAX PO (MULTI) detection reagent. For other detection reagents, optimization of staining conditions is recommended.. ...
Tytu : Osmotic concentration of gooseberry fruits - the influence of temperature, time, and pretreatment methods on mass transfer and total polyphenol and organic acid content. ...
In analytical sample preparation, extraction and leaching of chemical compounds from filters are major concerns, particularly when using liquid chromatography mass spectrometry (LCMS) for analysis. Explore our LCMS Sample Preparation solutions
A large amount of variation can be seen in each set of match samples, thus not allowing for differentiation between one producer and another. Sample preparation method 2 was devised to improve uniformity of the sample, and reduce variation. Sample preparation method 2 was used to prepare more samples for U1 and L1. U1 was chosen because there was a large variability in the samples. L1 was chosen because the sample were similar to U1. It was hoped using preparation method 2 the U1 samples would have less variation and be distinct from the L1 samples ...
In busy May, after the May 1st golden tourist season, the weather picked up. With the joint efforts of the military and civilians across the country, the epidemic situation has gradually improved, and the home has been isolated for many days, which has deepened the eagerness of the small partners for the scientific research. In the tide of resuming work and returning to school, you returned to the laboratory of your heart with excitement and started your favorite experimental career. Knowing something new, Abbkine experts have carefully created a set of experimental schemes for the pretreatment of WB experimental samples. With mature protein sample preparation methods, the WB experiment is more than half successful. Speaking of which, you may not be able to wait for our experimental plan. Next, our experimental plan will be presented to you:. ...
Rabbit Polyclonal C3 antibody [C3], C-term. Validated in WB, ICC/IF, IHC-P, IP, Immunosubtraction. Tested in Human, Mouse. Cited in 3 reference(s)., GeneTex products
One important observation about HCP immunoassays is that they occasionally give dilution-dependent results. Dilution dependence arises because the HCPs in the sample (perhaps one or a few HCPs) do not dilute in parallel with those in the standard (a mixture of 1000s of proteins). Limiting amounts of reagents can come in the form of coat, conjugate antibodies, or even Streptavidin-HRP that are associated with antigen excess and non-linear dilution behavior. It is advised to check all reagents to minimize impacts of reagent limitation sample dilution linearity. From a product purity perspective, however, sample dilution non-linearity is not an assay artifact but rather could be an important indication that a large amount of a particular HCP has co-purified with product. Phospholipase B-like 2 (PLBL2) was found to co-purify with multiple CHO-derived Mab products. It may be that this is a common impurity in Mabs produced across the biotech industry since it was found to have co-purified with ...
From just a few samples a week to hundreds a day, automation of NGS sample preparation allows you to reproducibly create high quality libraries for reliable next generation sequencing. See our co-developed, sequencing verified protocols for Illumina and IonTorrent automated sample preparation.
Sartorius optimizes HPLC sample preparation for time-saving solutions and reproducible and specific results from the preparation of solvents and standards to pipetting and filtration. Contact our experts today.
Find and compare a variety of chromatography sample preparation downloads on environmental xprt on the worlds largest environmental portal. View product and services catalogs, brochures, case studies, company news and more. Contact providers to ...
Everything relating to Sample Preparation System: compilations of facts, news, background knowledge, product information and market trends can be found here in the form of a comprehensive, up-to-date dossier.
Sample preparation makes things faster, easier, increases precision, uses fewer resources, and accurate results, which is popular in food testing labs
NIH Funding Opportunities and Notices in the NIH Guide for Grants and Contracts: Innovations in Cancer Sample Preparation (R21, R33, R21/R33) RFA-CA-07-003. NCI
Learn more about Sample Preparation. We enable science by offering product choice, services, process excellence and our people make it happen.
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Collaborate with our experts in 3D imaging, image processing and biological sample preparation to get the best out of your preclinical studies.
Integrated automated sample preparation and assay setup, with manual transfer to the detection unit, for all molecular testing needs ...
Integrated automated sample preparation and assay setup, with manual transfer to the detection unit, for all molecular testing needs ...
The importance of accurate sample preparation techniques cannot be overstated--meticulous sample preparation is essential. Often overlooked, it is the midway point where the analytes from the sample matrix are transformed so they are suitable for analysis. Even the best analytical techniques cannot rectify problems generated by sloppy sample pretreatment.Devoted entirely to teaching and reinforcing these necessary pretreatment steps, Sample Preparation Techniques in Analytical Chemistry addresses diverse aspects of this important measurement step. These include:* State-of-the-art extraction techniques for organic and inorganic analytes* Sample preparation in biological measurements* Sample pretreatment in microscopy* Surface enhancement as a sample preparation tool in Raman and IR spectroscopy* Sample concentration and clean-up methods* Quality control stepsDesigned to serve as a text in an undergraduate or graduate level curriculum, Sample Preparation Techniques in Analytical Chemistry also provides an
Lipids are involved in a host of biochemical and physiological processes in corals. Therefore, changes in lipid composition reflect changes in the ecology, nutrition, and health of corals. As such, accurate lipid extraction, quantification, and identification is critical to obtain comprehensive insight into a corals condition. However, discrepancies exist in sample preparation methodology globally, and it is currently unknown whether these techniques generate analogous results. This study compared the two most common sample preparation techniques for lipid analysis in corals: (1) tissue isolation by air-spraying and (2) crushing the coral in toto. Samples derived from each preparation technique were subsequently analysed to quantify lipids and their constituent classes and fatty acids in four common, scleractinian coral species representing three distinct morphotypes (Acropora millepora, Montipora crassotuberculata, Porites cylindrica, and Pocillopora damicornis). Results revealed substantial amounts
Microalgae have recently drawn attention as a potential resource for biofuels generation. In the short term, anaerobic digestion seems the most viable downstream process. Still, microalgae cell wall restrains the hydrolysis, hampering the methane production potential. This work aimed at improving the anaerobic biodegradability of microalgae through different pretreatment methods: microwave, low temperature and high temperature. For the microwave pretreatment, the specific energy was the main parameter affecting biomass solubilisation, increasing up to 800 % compared to untreated biomass. For the low and high temperature pretreatments, temperature was the most important factor, increasing biomass solubilisation up to 1190 and 2140 % in each case. BMP tests showed an increased methane yield after all pretreatments (40-80 %), with the highest results for the microwave pretreatment. In semi-continuous reactors operated at 15 days HRT, the methane production was increased by 30 and 33 % after ...
BACKGROUND: Minimal residual disease (MRD) assessment of hematopoietic neoplasia below 10-4 requires more leukocytes than is usually attainable by post-lysis preparation. However, not all laboratories are resourced for consensus Euroflow pre-lysis methodology. Our study aim was to validate a modified pre-lysis protocol against our standard post-lysis method for MRD detection of multiple myeloma (MM), chronic lymphocytic leukemia (CLL), and B-non Hodgkin lymphoma (B-NHL), to meet demand for deeper MRD assessment by flow cytometry.. METHOD: Clinical samples for MRD assessment of MM, CLL, and B-NHL (50, 30, and 30 cases, respectively) were prepared in parallel by pre and post-lysis methods for the initial validation. Total leukocytes, MRD, and median fluorescence intensity of antigen expression were compared as measures of sensitivity and antigen stability. Lymphocyte and granulocyte composition were compared, assessing relative sample processing stability. Sensitivity of the pre-lysis assay was ...
Woody biomass is one of the most abundant biomass feedstocks, besides agriculture residuals in the United States. The sustainable harvest residuals and thinnings alone are estimated at about 75 million tons/year. These forest residuals and thinnings could produce the equivalent of 5 billion gallons of lignocellulosic ethanol annually. Softwood biomass is the most recalcitrant biomass in pretreatment before an enzymatic hydrolysis. To utilize the most recalcitrant lignocellulosic materials, an efficient, industrially scalable and cost effective pretreatment method is needed. Obtaining a high yield of sugar from recalcitrant biomass generally requires a high severity of pretreatment with aggressive chemistry, followed by extensive conditioning, and large doses of enzymes. Catchlight Energys Sugar process, CLE Sugar, uses a low intensity, high throughput variation of bisulfite pulping to pretreat recalcitrant biomass, such as softwood forest residuals. By leveraging well-proven bisulfite technology and
Solid phase extraction has become a popular tool for analytical method development as a sample preparation technique. Solid phase sorbents can generally extract analytes based on polarity or selectively based on highly specific interactions between the analyte and an immobilized surface molecule. Biomolecular recognition based solid phases utilize antibodies, nucleic acids, and proteins as a secondary affinity layer on a solid phase support for selective analyte extractions and have been used for sample preparation, purification, separations, bioassays, and molecular diagnostics. Polymer supports for solid phase extraction are desirable for their low cost, abundance, chemical inertness, and pH stability. This thesis reports the use of capillary-channel polymer (C-CP) fibers as a solid support modified for selective extraction and on fiber fluorescence detection of nucleic acids and proteins. The polymer fibers are low cost and their unique shape allows them to be easily adapted into a fluidic fiber
Having to reach ever lower limits of detection is a daily challange in modern laboratories. In order to succeed in obtaining sufficiently sensitive analysis methods, sample preparation techniques such as Solid Phase Extraction (SPE) or Liquid-Liquid Extraction are often used as concentration steps. The concentration factor achieved in these cases depends on the amount of solvent used for analyte elution from the SPE cartridge or for liquid extraction. Following the extraction step, further concentration of analytes can be achieved by reducing the amount of solvent left in the extract. This is typically achieved by evaporation. Such a concentration step can contribute significantly to improved limits of detection for the overall analytical method. For the evaporative concentration step, commercially available rotary evaporators as well as custom solutions are widely used. These are mainly stand-alone systems for manual operation. The GERSTEL MultiPosition Evaporation Station (mVAP) in combination ...
Maura is a Marketing Manager for Biotage, where she develops and executes marketing strategy to support workflow solutions for applications in applied science market segments. Her prior roles include marketing, product management and technical applications at PerkinElmer, Thermo Fisher Scientific, and Teledyne Leeman Labs. These roles provided her with a significant amount of experience with sample preparation techniques and atomic spectroscopy applications in markets including: food and beverage, pharmaceutical, environmental, industrial and clinical ...
Singh, K.: New sample preparation techniques of macromolecular complexes for high resolution structure determination using cryo-EM. Dissertation, Georg-August-Universität, Göttingen (2019 ...
The Electron Microscopy Facility provides service, technical support, training and consultation to ensure efficient utilization of instrumental capabilities. Its staff also develops unique sample preparation techniques to assist scientists in obtaining their research objectives.. ...
In this study various pretreatment methods of sewage sludge inoculum and the statistical process optimization of de-oiled jatropha waste have been reported. Peak hydrogen production rate (HPR) and hydrogen yield (HY) of 0.36 L H2/L-d and 20 mL H2/g Volatile Solid (VS) were obtained when heat shock pretreatment (95 oC, 30 min) was employed. Afterwards, an experimental design was applied to find the optimal conditions for H2 production using heat-pretreated seed culture. The optimal substrate concentration, pH and temperature were determined by using response surface methodology as 205 g/L, 6.53 and 55.1 oC, respectively. Under these circumstances, the highest HPR of 1.36 L H2/L-d was predicted. Verification tests proved the reliability of the statistical approach. As a result of the heat pretreatment and fermentation optimization, a significant (~ 4 folds) increase in HPR was achieved. PCR-DGGE results revealed that Clostridium sp. were majorly present under the optimal conditions.
The three main components of wood, namely, cellulose, hemicellulose, and lignin, can be used in various areas. However, since lignin covalently crosslinks with wood polysaccharides creating networks that is an obstacle for extraction, direct extraction of different wood components in high yield is not an easy matter. One potential approach to overcome such obstacles is to treat the wood with specific enzymes that degrade the networks by specific catalysis. However, the structure of wood is so compact that the penetration of the wood fibers by large enzyme molecules is hindered. Thus, the pretreatment of wood prior to the application of enzymes is necessary, for opening the structure.. One pretreatment method that was performed in this thesis is based on kraft pulping, which is a well-established and industrialized technique. For untreated wood, the wood fibers cannot be attacked by the enzymes. A relatively mild pretreatment was sufficient for wood polysaccharides hydrolyzed by a culture ...
Extrusion pretreatment of lignocellulosic biomasses is a physical continuous pretreatment method for subsequent fermentative ethanol production. Twin-screw elements (conveying, kneading and reverse) have different effects on enzymatic digestibility according to their geometries and functions. The first study evaluated the effects of individual functional screw elements on enzymatic digestibility and found that extrusion enhanced enzymatic digestibility under all tested condition. Reverse and kneading screw elements however resulted in lignin re-distribution over the cellulose fibres, blocked pores (shown via SEM) and resulted in less digestible biomass, compared to conveying screw elements, only. Lignin removal via NaOH reversed this effect and the highest digestibility was found for biomass extruded in the presence of reverse screw elements (the most severe conditions tested in this study). Based on these finding a process was developed for separating xylose from lignocellulosic biomass (steam-exploded
It is recommended to pretreat Passiflora seeds before planting. They contain a hard seed coat and are very slow to sprout. There are various pretreatment methods, but the simplest is to soak the seeds for 24-48 hours in warm to the touch water, just prior to planting. Optionally, seeds can be lightly scarified with sand paper to provide some permeation on the seed coat ...
The Laser Ablation of Microparticle Aerosol (LAMA) process provides a method for generating high yields of aerosolized nanoparticles (NPs) that are then accelerated through a nozzle and impacted onto substrates. The process allows nanostructured films and patterned lines to be directly written onto substrates at room temperature. Using the LAMA process, we have demonstrated the ability to produce thick patterned deposits with densities ranging from 60-70% in the as-deposited state. After deposition, the lines were densified using pressure-assisted sintering and the microstructures and strengths of the films were investigated. Pressure-enhanced sintering resulted in densification and strong bonding at processing temperatures of 100-175°C and pressures of 100-600 MPa. Mechanisms for sintering at low temperatures are discussed. ...
Early buyers will receive 10% customization on reports.. Sample preparation is one of the most important operations when it comes to analysis of a sample. This procedure consumes around 60% of the total workload of labs. There are various steps involved in sample preparation, and following these steps is mandatory as the sample preparation process might become complicated if not executed properly with utmost caution. Sample preparation market has highlighted greater adoption of sample preparation instruments that not only simplify the analytical tasks, but also save considerable amount of time. The major applications of electron microscopy & sample preparation are in the life sciences and material sciences domains. Increasing life science applications of electron microscope and R&D expenditure across varied regions around the world have resulted in the overall growth of the electron microscopy and sample preparation market.. Another major factor driving the global electron microscopy & sample ...
Fernández-Cid, A.*, Riera, A.*, Tognetti, S., Herrera, M. C., Samel, S., Evrin, C., Winkler, C., Gardenal, E., Uhle. S., Speck, C. (2013). An ORC/Cdc6/MCM2-7 complex is formed in a multistep reaction to serve as a platform for MCM double-hexamer formation. Molecular Cell 50, 577-588 ...
The invention relates to a preparation method of a CZTS (copper-zinc-tin-sulfur) quaternary target. According to the preparation method, high-purity Cu2S powder, ZnS powder and SnS2 powder are taken as raw materials and subjected to sufficient ball milling and uniform mixing in the stoichiometric ratio, the mixed powder is placed in a mold and subjected to hot pressed sintering forming, and the CZTS quaternary target is prepared. The preparation method is simple in process, the prepared CZTS quaternary target has uniform ingredients, the relative density is higher than 90%, the sputtering performance is excellent, and the method is mainly used for preparation and large-scale production of high-efficiency and low-cost CZTS thin-film solar cells.
The Sample Preparation Laboratories provide wet lab facillities for Sthe SSRL User Program. The labs support final stage sample preparations and straight-forward laboratory manipulations in Biology, Chemistry, Materials Science and the Geosciences. SSRL houses two BioChemMat-focused Laboratories (BCM1 & 2) and one Geoscience-focused Laboratory. All Sample Preparation Laboratories are potentially available to all users.. ...
Selected T1 characteristics of the full sample (n = 1,709), the at-risk sample (n = 1,564), and the analytic sample (n = 1,139) are displayed in Table 1. An equal percentage of respondents in their 40s, 50s, and 60s were recruited at T1. The T1 cohort was predominantly white, married, and employed, had studied beyond high school, and had generally high household incomes. The 1,139 men in the analytic sample were significantly more likely to be younger, white, married, and employed and to have higher education and household incomes (all P ≤ 0.003) than the remaining 425 at-risk men not in the analytic sample.. Table 2 shows the distribution of sleep duration in the at-risk sample, the analytic sample, and those not in the analytic sample. Although there were small differences in the distribution of this variable between those in and not in the analytic sample, the difference was not statistically significant (χ42, P = 0.06).. There were a total of 90 cases of diabetes during 14,737 ...
Selected T1 characteristics of the full sample (n = 1,709), the at-risk sample (n = 1,564), and the analytic sample (n = 1,139) are displayed in Table 1. An equal percentage of respondents in their 40s, 50s, and 60s were recruited at T1. The T1 cohort was predominantly white, married, and employed, had studied beyond high school, and had generally high household incomes. The 1,139 men in the analytic sample were significantly more likely to be younger, white, married, and employed and to have higher education and household incomes (all P ≤ 0.003) than the remaining 425 at-risk men not in the analytic sample.. Table 2 shows the distribution of sleep duration in the at-risk sample, the analytic sample, and those not in the analytic sample. Although there were small differences in the distribution of this variable between those in and not in the analytic sample, the difference was not statistically significant (χ42, P = 0.06).. There were a total of 90 cases of diabetes during 14,737 ...
In a normal day at Ginkgo, we sequence DNA from thousands of different bacterial, fungal, and plant samples thanks to our automated sample preparation methods and terabyte-scale data de-multiplexing algorithms. These tools make it possible for us to quickly shift to sequencing up to ten thousand small viral genomes every day.. We can start from already extracted RNA from clinical labs, or use our automated sample preparation system and standard methods to extract RNA from patient samples or environmental swabs. Viral RNA would be amplified by reverse transcription using virus-specific primers (with human-specific primers serving as an internal positive control) to generate material suitable for our high-throughput NGS process. Our existing bioinformatics pipeline would be extended to quantify virus in each sample, report any failures to detect the positive control (indicating a sample which must be re-queued), and assemble the detected virus sequences and format them for submission to public ...
Påliteligheten av resultatene i metabolomics eksperimenter avhenger effektiviteten og reproduserbarhet av prøveopparbeidelse....
The murine intestinal tract represents a difficult organ system to study due to its long convoluted tubular structure, narrow diameter, and delicate mucosa which undergoes rapid changes after sampling prior to fixation
A fully automated sample preparation method has been developed and tested for extraction and analysis of drugs of abuse from hair samples. Click to read more...
48Hour Discovery. Using its library of more than a billion molecules, along with bioinformatics and proprietary techniques developed by chemistry professor Ratmir Derda and his team, 48Hour Discovery provides ligand screening services at an unprecedented turnaround time. The companys unique approach has the potential to empower academic research and to unlock new discoveries for the pharmaceutical and biotechnology industries.. Amplicet. Oncology researcher Linda Pilarski spent the last 15 years developing a DNA detection system and a capillary sample cassette that combine to create cassette PCR: a rapid, robust, user-friendly technology that detects harmful bacteria in seven hours, compared with standard testing lab times of 24 to 48 hours. The Amplicet research team also developed a fast, sensitive sample preparation method that allows several tests for specific problematic microbes in industry settings, such as food pathogens like E. coli and corrosion-promoting bacteria.. BioImmuno ...
Science Archive. Dec. 22, 2014Science Scanning Probe Microscopy Collagen Surface Functionalization We tested several sample preparation methods for collagen surfaces, suitable for Single Molecule
Eliot Colemans 6-Step Bed Preparation Method - Follow these steps to prepare your beds and ensure vigorous growth. Field-tested tools from Johnnys Selected Seeds.
Our high-purity reagents for AAS sample preparation comprise Suprapur® salts, matrix modifiers, ionization buffers, and reagents for determining mercury.
Sample preparation is often the first step in an analysis; the result of this step can affect the rest of the analytical process. You need a sample that is representative, reproducible, homogenous, and suitable for column injection or other assay. Agilent sample preparation products get your samples ready for analysis, giving you sample aliquots that are compatible with your separation and detection methods. Agilent Bond Elut SPE, Chem Elut, Plexa and Captiva are among the many well trusted names supported by years of usage and peer reviewed studies ...
Described is a system and method for in situ sample preparation and imaging. The system includes a multi-axis stage 100 having a bulk stage 110 and a grid stage 150 with various degrees of freedom to allow for sample preparation. In some embodiments, a focused ion beam system is used to prepare a lamella on the bulk stage 110. The lamella can then be transferred to the grid stage 150 from the bulk stage 110 without needing to move the multi-axis stage 100 from the focused ion beam system.
Updated Parameter (2625) Plasma dilution in Procedure (183) Insulin Blood Level in Pipeline (7) IMPC Pipeline to version 1.2. name changed from Plasma dilution to Sample dilution. minor_version changed from 1 to 2. description changed from plasma_dilution to sample_dilution ...
Course/education, 2018-02-20, Basic Course in SEM and TEM (1 ECTS), This course covers fundamental theory and operational principles as well as specimen preparation techniques for scanning and transmission electron microscopy (SEM/TEM).
From easy Crêpe recipes to masterful Crêpe preparation techniques, find Crêpe ideas by our editors and community in this recipe collection.
From easy Summer Salad recipes to masterful Summer Salad preparation techniques, find Summer Salad ideas by our editors and community in this recipe collection.
Reliable, efficient and easy sample preparation is one of the most crucial steps for achieving optimal and reproducible results in all tasks of protein and nucleic acid analysis.. Raw materials of premium quality, in-house stringent quality control and application tests guarantee best results with SERVA reagents, solutions and kits.. The right solution for the complete workflow - from extraction of proteins or nucleic acids to quantification :. Extraction ...
Reliable, efficient and easy sample preparation is one of the most crucial steps for achieving optimal and reproducible results in all tasks of protein and nucleic acid analysis.. Raw materials of premium quality, in-house stringent quality control and application tests guarantee best results with SERVA reagents, solutions and kits.. The right solution for the complete workflow - from extraction of proteins or nucleic acids to quantification :. Extraction ...
Health, ...PHOENIX Ariz. June 23 2011 A new automated sample preparation syst...The fully automated integrated high-throughput system is a key tool ...The platform can prepare nearly 1000 samples in less than 24 hours in...The scientific leadership for the integrated system specification and ...,TGen,,Tecan,and,ASU,Biodesign,Institute,develop,sample,preparation,solution,for,proteomic,research,medicine,medical news today,latest medical news,medical newsletters,current medical news,latest medicine news
You can download our full sample preparation products catalog and use the catalog numbers to place orders. For more information about the products, email: [email protected] ...
The global sample preparation market was valued at US$ 5.7 billion in 2017 and is expected to witness a CAGR of 6.3% over the forecast period (2017â€
Sample application systems can include an extraction mechanism to remove a sample from sample containers, a sample vessel disposed on a deployment mechanism, where the deployment mechanism is arrange
Incubation of cells with FcR Blocking Reagent, mouse, increases the specificity of labeling with MACS® Antibodies or MACS MicroBeads and thereby improves the specificity of immunofluorescent staining and purity of magnetically isolated target cells, respectively. - France
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ProLab Systems is a dynamic company. We specialize in providing total laboratory solutions and support. Our strongest asset is our highly professional team.. ...
4) Sample phenotype file in tab-delimited with a minimum of 4 columns, 1) sample names; 2) short name of samples; 3) groups; 4) samples to include in the analysis; 5) any factors that could influence any bias such as library protocol or preparation method. The first 4 columns are mandatory and should strictly match the names and order as mentioned below (names case-sensitive), column 5-11 are optional and could be left ...
Advanced Cell Diagnostics RNAscope® technology can be applied to any gene and any species, appropriate sample preparation is essential.
Review tips and techniques on how to get the best out of your IHC experiments with our resident specialists, including sample preparation, immunostaining protocol, suggestions for alternative
Section C: This section was purely administrative Compilation and preparation of steganography methods of all types, in ... Requisitions for sampling check-up on German signal communication and evaluation of observations, in collaboration with Gen ... Its primary function was the direction of analytic operations of the service and consisted of the following groups: Group I ... New methods were developed to utilise the results of the point-to-point intercept of traffic by W-Leit, Southeast, and the air- ...
Students who do not use these courses often rely on material from university text books, GRE preparation books, sample tests, ... Critics have also stated that the computer-adaptive method of placing more weight on the first several questions is biased ... and 423 on the analytic, while white test-takers averaged 496, 538, and 564, respectively. The National Association of Test ... ETS provides preparation software called PowerPrep, which contains two practice tests of retired questions, as well as further ...
Chemical and physical characteristics of samples are studied and analyzed by the center. "Building Technologies" is created for ... The university has the Foundation Faculty, which is an intensive academic, scientific, and language preparation of students for ... "IRGETAS" is a common use center for carrying out analytic and materials engineering research. ... ", "Digital Earth Exploration Methods", "Precision Farming", with participation of the Technical University of Munich, Obuda ...
... the relative differences in expression among different spots within a sample and between samples is the preferred method of ... It is critical that information about the sample preparation and handling is discussed, in order to help identify the ... Class discovery analysis: This analytic approach, sometimes called unsupervised classification or knowledge discovery, tries to ... The two samples to be compared (pairwise comparison) are grown/acquired. In this example treated sample (case) and untreated ...
A more common method directly measures TOC in the sample by again acidifying the sample to a pH value of two or less to release ... Since the early 1970s, TOC has been an analytic technique used to measure water quality during the drinking water purification ... As many of these biotechnology operations include the preparation of medicines, the U.S. Food and Drug Administration (FDA) ... of sample can be handled making the methods less sensitive than chemical oxidation methods capable of digesting as much as 10 ...
Other analytical methods[edit]. Other analytic methods can be subdivided into the physical, the gas chromatographical, and the ... Sampling from area sources[edit]. There are two main odor sampling techniques: direct and indirect odor sampling techniques. ... He suggested the evolutionary pressure of diversification of food sources and increased complexity of food preparation ... To collect an odor sample, the samples must be collected using specialized sample bags, which are made from an odor free ...
However, they all require long sample preparation times and destroy the sample. Nondestructive techniques for the forensic ... Elemental Impurities-Procedures, will increase the need for ICP-MS technology, where, previously, other analytic methods have ... Additionally, increases in sample throughput from dozens of samples a day to hundreds of samples a day has revolutionized ... For other applications that may involve very viscous samples or samples that have particulate matter, a process known as sample ...
Then, various methods used to characterize organogels will be explained. Finally, we will review the use of organogels in ... Souge, J. L. Analytic solutions to Smoluchowski's coagulation equation: a combinatorial inter-pretation. J. Phys. A.: Math. Gen ... The tip is lowered into the sample and a laser reflecting off the cantilever allows for precise measurements to be obtained. In ... Koshima, H.; Matsusaka, W. Yu, H. Preparation and photoreaction of organogels based on ben-zophenone. J. Photochemistry and ...
Other analytic methods can be subdivided into the physical, the gas chromatographical, and the chemosensory method. When ... To collect an odor sample, the samples are collected using specialized sample bags, which are made from an odor free material, ... He suggested the evolutionary pressure of diversification of food sources and increased complexity of food preparation ... The method is based on dilution of an odor sample to the odor threshold. The numerical value of the odor concentration is equal ...
National Inpatient Sample (NIS) (formerly the Nationwide Inpatient Sample): A 20 percent stratified sample of all-payer, ... HCUP Methods Series Reports offer methodological information on the HCUP databases and software tools. HCUP Findings-At-A- ... ICD-9-CM codes were frozen in preparation for ICD-10-CM implementation and regular maintenance of the codes has been suspended ... While designed to be used with HCUP databases, the analytic tools may be applied to other administrative databases. The ...
May 2018). "Cochrane Qualitative and Implementation Methods Group guidance series-paper 5: methods for integrating qualitative ... He used a meta-analytic approach to aggregate the outcomes of multiple clinical studies. In 1972 Archie Cochrane wrote: 'It is ... Recent visualisation innovations include the albatross plot, which plots p-values against sample sizes, with approximate effect ... provides guidance to authors for the preparation of Cochrane Intervention reviews.' The Cochrane Handbook also outlines the key ...
Out of a sample of 200, 100 of whom were dependent on cannabis and the other 100 emotionally healthy, the dependent group ... Meta-analytic evidence confirms that self-reported emotional intelligence predicting job performance is due to mixed EI and ... Since its popularization in recent decades, methods of developing EI have become widely sought by individuals seeking to become ... that several participants were good leaders because their emotional intelligence was developed in counselor preparations, where ...
Eventually, the manual method was modified so that a match could be notated in-match at courtside directly into a microcomputer ... But how many is enough? In the literature there are large differences in sample sizes. These problems have very serious direct ... This modelling of technical attainment has been replicated in many sports and form the basis of preparation at the highest ... The modelling of competitive sport is an informative analytic technique because it directs the attention of the modeller to the ...
R&D head Methods' development of the organization scintillation properties for single crystals on the basis of rare earth ... The reference book contains a brief biographical information on contemporary Ukrainian artists and samples of their signatures ... "Analytic geometry" (2003) (ISBN 978-966-8319-92-1). He is the initiator of the creation of a new scientific journal "Functional ... extreme urgency acquire developed a new organic and inorganic scintillators and processes for their preparation. Custom ...
Hence, the latter method may be used on samples that test positive using other methods to help distinguish between the various ... Since the first preparation was reported in 1887, numerous synthetic routes to amphetamine have been developed. The most common ... Several other studies,[97-101] including a meta-analytic review[98] and a retrospective study,[97] suggested that stimulant ... method 5). Another method is the reaction of phenylacetone with ammonia, producing an imine intermediate that is reduced to the ...
This method is preferred among most over the more traditional chromic acid bath for cleaning NMR tubes, because no traces of ... The preparation of aqua regia by directly mixing hydrochloric acid with nitric acid only became possible after the discovery in ... Aqua regia is also used in etching and in specific analytic procedures. It is also used in some laboratories to clean glassware ... Hoffman, R. (10 March 2005) How to make an NMR sample, Hebrew University. Accessed 31 October 2006. American Industrial Hygiene ...
... are the set of presplit sample indices, set of sample indices for which the split test is true, and set of sample indices for ... Decision tree learning is a method commonly used in data mining. The goal is to create a model that predicts the value of a ... Requires little data preparation. Other techniques often require data normalization. Since trees can handle qualitative ... what you need to know about data mining and data-analytic thinking]. Fawcett, Tom. (1st ed.). Sebastopol, Calif.: O'Reilly. ...
New methods of visualizing the activity of the brain, such as PET scans and CAT scans, began to exert their influence as well, ... Inherent in his analytic approach are the concepts of the phoneme, the morpheme and the root. The grammar of TAMIL language ... He was also the first to recognise that amber was a fossilized resin from pine trees because he had seen samples with trapped ... The Hindus excel in the manufacture of iron, and in the preparations of those ingredients along with which it is fused to ...
Reich sent a sample of the bacteria to a Norwegian biologist, Theodor Thjøtta of the Oslo Bacteriological Institute, who also ... Freud allowed him to start meeting with analytic patients in September that year, although Reich was just 22 years old and ... In 1937 the Norwegian pathologist Leiv Kreyberg was allowed to examine one of Reich's bion preparations under a microscope. ... His second wife, Ilse Ollendorf, said vegetotherapy replaced the psychoanalytic method of never touching a patient with "a ...
The Manor had been built as a hunting lodge in 1799 and was improved by King in preparation for their honeymoon. It later ... The notes are around three times longer than the article itself and include (in Note G), in complete detail, a method for ... Babbage was impressed by Lovelace's intellect and analytic skills. He called her "The Enchantress of Number." In 1843, he wrote ... Bromley notes several dozen sample programs prepared by Babbage between 1837 and 1840, all substantially predating Lovelace's ...
The focus is general methods that can be applied in various problem domains. M&S Applications solve real world problems by ... Examples are supporting deep water operation of the US Navy or the simulating the surface of neighbored planets in preparation ... 2013). "Simulation Study to Validate Sample Allocation for the National Compensation Survey" (PDF). JSM 2013 - Survey Research ... in order to blend algorithmic and analytic techniques through visualizations available directly to decision makers. A study ...
However, pure calcium cannot be prepared in bulk by this method and a workable commercial process for its production was not ... Other calcium preparations include calcium carbonate, calcium citrate malate, and calcium gluconate. The intestine absorbs ... Calcium is readily complexed by oxygen chelates such as EDTA and polyphosphates, which are useful in analytic chemistry and ... in a sample compared to the same ratio in a standard reference material. 44Ca/40Ca varies by about 1% among common earth ...
... analytic number theory) Malgrange preparation theorem (singularity theory) Malgrange-Ehrenpreis theorem (differential equations ... Nyquist-Shannon sampling theorem (information theory) Odd number theorem (physics) Open mapping theorem (complex analysis) Open ... voting methods) Girsanov's theorem (stochastic processes) Glaisher's theorem (number theory) Gleason's theorem (Hilbert space) ... analytic geometry) Cramér-Wold theorem (measure theory) Critical line theorem (number theory) Crooks fluctuation theorem ( ...
The infrared energy from the filament heats the sample. Ultra-high vacuum is necessary for many surface analytic techniques ... These include simple air leaks, virtual leaks, and desorption (either from surfaces or volume). A variety of methods for leak ... Thin film growth and preparation techniques with stringent requirements for purity, such as molecular beam epitaxy (MBE), UHV ... The manipulator or sample holder may include features that allow additional control and testing of a sample, such as the ...
... and Sample Phonics Lessons. Synthetic phonics, also known as blended phonics, is a method employed to teach students to read by ... It compared analytic Phonics with synthetic Phonics and advantaged students with disadvantaged children. The report found that ... preparation and support for teachers. The review summarizes the findings of 107 studies of early grade literacy interventions ( ... For some teachers this is a method of teaching spelling by using the sounds (phonemes). However, it can also be a method of ...
Sackett, D. L. (1979). "Bias in analytic research". Journal of Chronic Diseases. 32 (1-2): 51-63. doi:10.1016/0021-9681(79) ... The study reported positive results as the test results for each sample were consistent with the healers intention that healing ... Placebo - an inert medicine or preparation which works because the patient thinks it will ... Sackett (1979) catalogued 56 biases that can arise in sampling and measurement in clinical research, among the above-stated ...
The psycho-molecular method uses specific tasks in order to influence specific neurotransmitter systems. Through the control of ... Nearest-neighbor comparison of two persons in a sample involves comparing a person A with the person B who has the closest ... Saulsman, L. M.; Page, A. C. (2004). "The five-factor model and personality disorder empirical literature: A meta-analytic ... that involves living at a farm where the sole activity is food foraging and preparation; Expressive technique, that involves ...
SAR and ISAR are means of combining multiple radar samples, taken over time, to create the effect of a much larger antenna, far ... This method improved sensitivity for poorer time resolution, because correlated peaks are twice the width of uncorrelated peaks ... Proper site selection and preparation is necessary for all types. Proper planning includes avoiding clutter sources such as ... Methodology and Analytic Formulation" (PDF). CCDC Army Research Laboratory. McCorkle, John (15 November 1993). "Early results ...
... and Sample Phonics Lessons. Analogy phonics is a particular type of analytic phonics in which the teacher has students analyze ... method or whole-word method, are not a part of the phonics method. They are usually associated with whole language and balanced ... reimagined teacher preparation, and leadership); adaptive teaching (recognizing the student's individual, culture and ... For some teachers this is a method of teaching spelling by using the sounds (phonemes). However, it can also be a method of ...
Rieke, Reuben D.; Wu, Tse-Chong; Rieke, Loretta I. (1995). "Highly reactive calcium for the preparation of organocalcium ... Analytic Chemistry, volume 55, issue 2, pages 280-286 doi:10.1021/ac00253a023 ... Evaluation of the PAH distributions using statistical methods such as principal component analyses (PCA) enabled the study to ... "Comparison of Batch Mode and Dynamic Physiologically Based Bioaccessibility Tests for PAHs in Soil Samples" (PDF) ...
Bailey, Christopher H (January 2014). "Improved meta-analytic methods show no effect of chromium supplements on fasting glucose ... Its Mohs hardness is 8.5, which means that it can scratch samples of quartz and topaz, but can be scratched by corundum. ... "Preparation, Structure and Vibrational Spectroscopy of Tetraperoxo Complexes of CrV+, VV+, NbV+ and TaV+". PhD thesis, ... Methods to Develop Inhalation Cancer Risk Estimates for Chromium and Nickel Compounds. Research Triangle Park, NC: U.S. ...
Reilly T, Ekblom B (June 2005). "The use of recovery methods post-exercise". J. Sports Sci. 23 (6): 619-27. doi:10.1080/ ... To identify which public health strategies are effective, a Cochrane overview of reviews is in preparation.[131] ... For instance, there are deficiencies in methodological planning such as uncontrolled nonrandomized trials, small sample sizes, ... "A meta-analytic review of the effects of exercise on brain-derived neurotrophic factor". J Psychiatr Res. 60C: 56-64. doi ...
Other analytical methodsEdit. Other analytic methods can be subdivided into the physical, the gas chromatographical, and the ... Sampling from area sourcesEdit. There are two main odor sampling techniques: direct and indirect odor sampling techniques. ... He suggested the evolutionary pressure of diversification of food sources and increased complexity of food preparation ... To collect an odor sample, the samples must be collected using specialized sample bags, which are made from an odor free ...
MethodEdit. Reminiscence therapy is often used in a nursing home or "geriatric health facility."[12][13][14][15] The structure ... death preparation all remained to be improved from pre treatment.[40] Improvements comparable to those found in the elderly ... Problem focused coping involves the individual in a goal-oriented effort to alter the situation with an analytic approach to ... although a few studies have looked at other elderly samples.[2] ... preparation for death all showed improvements after the therapy ...
... and analytic methods (characterization methods such as electron microscopy, X-ray diffraction, calorimetry, nuclear microscopy ... Because of this, the powder diffraction method, which uses diffraction patterns of polycrystalline samples with a large number ... This involves methods such as diffraction with X-rays, electrons, or neutrons, and various forms of spectroscopy and chemical ... Also, chemical and physical methods are also used to synthesize other materials such as polymers, ceramics, thin films, etc. As ...
Method biasEdit. Response bias is another cross-cultural difference that has been shown to affect how we measure constructs and ... A sample of intention behind questions asked for understanding observable responses, displayed character, and underlying ... Wiesner, W. H.; Cronshaw, S. F. (1988). "A meta-analytic investigation of the impact of interview format and degree of ... Maurer, T.; Solamon, J.; Andrews, K.; Troxtel, D. (2001). "Interviewee coaching, preparation strategies and response strategies ...
Methods[edit]. Laboratory testing[edit]. Investigations of the self-serving bias in the laboratory differ depending on the ... Campbell, W. Keith; Sedikides, Constantine (1999). "Self-threat magnifies the self-serving bias: A meta-analytic integration". ... a student who attributes earning a good grade on an exam to their own intelligence and preparation but attributes earning a ... This method can be used for numerous outcome variables to determine the presence or absence of the self-serving bias. ...
... fair sampling procedures, blinding of studies, and other methods. All gathered data, including the experimental or ... Homeopathic preparation Rhus toxicodendron, derived from poison ivy.. See also: List of topics characterized as pseudoscience ... Logical positivism / analytic philosophy. *Positivist school. *Postpositivism. *Sociological positivism. *Machian positivism ( ... "Against Method: Outline of an Anarchistic Theory of Knowledge. ISBN 978-0-86091-646-8. Archived from the original on 2007-12-12 ...
Kurtz, Ron (1990). Body-Centered Psychotherapy, The Hakomi Method. LifeRhythm.. *^ Schwartz, Richard C. (2013). Internal Family ... for providing more methodological details in reported studies and for the use of larger sample sizes.[3][29] ... samatha is used as a preparation for vipassanā, pacifying the mind and strengthening the concentration in order to allow the ... "The effect of mindfulness-based therapy on anxiety and depression: A meta-analytic review". Journal of Consulting and Clinical ...
Other calcium preparations include calcium carbonate, calcium citrate malate, and calcium gluconate.[5] The intestine absorbs ... Trypsin, a digestive enzyme, uses the first method; osteocalcin, a bone matrix protein, uses the third. Some other bone matrix ... in a sample compared to the same ratio in a standard reference material. 44Ca/40Ca varies by about 1% among common earth ... which are useful in analytic chemistry and removing calcium ions from hard water. In the absence of steric hindrance, smaller ...
Fertility awareness methods that rely on cycle length records alone are called calendar-based methods.[40] Methods that require ... and for the preparation of the uterus for pregnancy.[1] Up to 80% of women report having some symptoms during the one to two ... "Do women's mate preferences change across the ovulatory cycle? A meta-analytic review". Psychological Bulletin. 140 (5): 1205- ... "Ovarian hormones and binge eating: exploring associations in community samples". Psychological Medicine. 38 (12): 1749-1757. ...
Rieke, Reuben D.; Wu, Tse-Chong; Rieke, Loretta I. (1995). "Highly reactive calcium for the preparation of organocalcium ... Analytic Chemistry, volume 55, issue 2, pages 280-286 doi:10.1021/ac00253a023 ... Evaluation of the PAH distributions using statistical methods such as principal component analyses (PCA) enabled the study to ... "Comparison of Batch Mode and Dynamic Physiologically Based Bioaccessibility Tests for PAHs in Soil Samples" (PDF) ...
Flowback and Produced water ... Chesapeake agrees that an indepth study of toxicity, the development of new analytic methods ... and the agency in assessing risks of stormwater and wastewater overflows and enables better sampling, analytical methods, and ... and required the preparation of an annual environmental report.[16][17][18][19] ... I began issuing treatment methods and levels of requirements in 1986 and these are continually adapted to new hazardous wastes ...
Over the following years the number of MDMA samples increased, eventually exceeding the number of MDA samples in the early ... There are numerous methods available to synthesize MDMA via different intermediates.[107][108][109][110] The original MDMA ... Köllisch developed a preparation of a hydrastinine analogue, methylhydrastinine, at the request of fellow lab members, Walther ... Laws KR, Kokkalis J (August 2007). "Ecstasy (MDMA) and memory function: a meta-analytic update". Human Psychopharmacology. 22 ( ...
Flowback and Produced water ... Chesapeake agrees that an indepth study of toxicity, the development of new analytic methods ... water and sediment sampling instruments used in study of ocean and coastline. One of the major missions of the Bold was to ... and required the preparation of an annual environmental report.[15][16][17][18] ... the development of new analytical methods and tracers are not practical given the budget and schedule limitation of the study." ...
Diagnostic method. Based on observed behavior, reported experiences, and reports of others familiar with the person[5]. ... For people who are unwilling or unable to take medication regularly, long-acting depot preparations of antipsychotics may be ... representative sample from a 1999 study, 12.8% of Americans believed that individuals with schizophrenia were "very likely" to ... "Facial emotion perception in schizophrenia: a meta-analytic review". Schizophrenia Bulletin. 36 (5): 1009-19. doi:10.1093/ ...
Marketers of cosmetics and skincare preparations regularly provide fashion editors with free samples in the hope that their ... In other words, the decoy price assists in framing value.[163] Marketers use a variety of methods to frame value: e.g. quote ... Eisend, Martin; Stokburger-Sauer, Nicola E. (27 February 2013). "Brand personality: A meta-analytic review of antecedents and ... Sampling or Limited-scale Trial: Where practical, obtaining samples, free trial or a 'test-drive' prior to purchase ...
The "Platform Test" method involves having the candidate make a presentation to both the selection panel and other candidates ... ISBN 978-0-19-993069-2. Wiesner, Willi H.; Cronshaw, Steven F. (December 1988). "A meta-analytic investigation of the impact of ... Sometimes other selection tools (e.g., work samples, cognitive ability tests, personality tests) are used in combination with ... preparation strategies, and response strategies in relation to performance in situational employment interviews: An extension ...
Bolzano, "On the Mathematical Method", §2 Bolzano, "On the Mathematical Method", §3 Bolzano, "On the Mathematical Method", §4 ... He also gave the first purely analytic proof of the intermediate value theorem (also known as Bolzano's theorem). Today he is ... 103 Volumes available, 28 Volumes in preparation). Wissenschaftslehre, 4 vols., 2nd rev. ed. by W. Schultz, Leipzig I-II 1929, ... a sample of a new way of developing analysis", whose ultimate goal would not be realized until some fifty years later when they ...
Problem focused coping involves the individual in a goal-oriented effort to alter the situation with an analytic approach to ... preparation for death all showed improvements after the therapy was concluded. At a follow up depression, other indicators of ... although a few studies have looked at other elderly samples. Research and implementation has been tried in several areas with ... due to the increased organization of methods and purpose of this therapy. The benefits of reminiscence therapy occurs ...
The cryptanalytic methods employed by the Vauck section differed from normal methods of solution because of the peculiar nature ... The In 7/IV unit Hollerith kit was used at the request of the OKW/Chi for instance, when for example, the preparation of 3- ... The recipher consists of two elements: Shifting by means of Chiffranten enciphering: Any figure, chosen from Row A (sample code ... termed Rapid analytic machinery that were time-consuming to build and costly, and only worked in specifically defined areas, ...
Homeopaths contend that their methods produce a therapeutically active preparation, selectively including only the intended ... Sample I (July 21, 2008). "Pharmacists urged to 'tell the truth' about homeopathic remedies". The Guardian. London. Malik, IA; ... Sackett, David L. (1979). "Bias in analytic research". Journal of Chronic Diseases. 32 (1-2): 51-63. doi:10.1016/0021-9681(79) ... Some homeopathic preparations involve poisons such as Belladonna, arsenic, and poison ivy, which are highly diluted in the ...
Initial attempts employed exfoliation techniques similar to the drawing method. Multilayer samples down to 10 nm in thickness ... and the surprisingly easy preparation method that they described, sparked a "graphene gold rush". Research expanded and split ... The Kubo formula provides an analytic expression for the graphene's conductivity and shows that it is a function of several ... The method can control thickness, ranging from monolayer to multilayers, which is known as "Tang-Lau Method". Gram-quantities ...
2018) reserved 10% of positive training earthquake data samples and an equal quantity of randomly chosen negative samples. ... A deep study of the RTP method in 2008 found that out of some twenty alarms only two could be considered hits (and one of those ... 2012 concluded: "The concept of a large-scale 'preparation zone' indicating the likely magnitude of a future event, remains as ... Meta-Analytic Insight on their Limitations". arXiv:1910.01178. doi:10.1785/0220200021. S2CID 203642017. Cite journal requires , ...
Experimental methods using randomised samples, though, suggest that mindfulness as a state or temporary practice can influence ... Schutte, Nicola S.; Malouff, John M. (April 2014). "A meta-analytic review of the effects of mindfulness meditation on ... According to the contemporary Theravada orthodoxy, samatha is used as a preparation for vipassanā, pacifying the mind and ... methods and adds in newer psychological strategies such as mindfulness and mindfulness meditation. Cognitive methods can ...
Analytic Sample Preparation Methods * Animals * Chromatography, Liquid * Databases, Factual * Gas Chromatography-Mass ... NMR and MS methods for metabonomics Methods Mol Biol. 2011;691:385-415. doi: 10.1007/978-1-60761-849-2_24. ... starting from sample preparation, to determining the measurement details of all analytical platforms, and finally, to ...
... filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry ... Universal sample preparation method for proteome analysis Nat Methods. 2009 May;6(5):359-62. doi: 10.1038/nmeth.1322. Epub 2009 ... Analytic Sample Preparation Methods / methods* * Animals * Brain Chemistry * Endopeptidases / chemistry * HeLa Cells ... We describe a method, filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion ...
Analytic Sample Preparation Methods. Use of various chemical separation and extraction methods, such as SOLID PHASE EXTRACTION ... An extraction method that separates analytes using a solid phase and a liquid phase. It is used for preparative sample cleanup ... CHROMATOGRAPHY; and SUPERCRITICAL FLUID EXTRACTION; to prepare samples for analytical measurement of components. ...
Analytic Sample Preparation Methods. Use of various chemical separation and extraction methods, such as SOLID PHASE EXTRACTION ... protein (WMSP) obtained by ultrasound-assisted extraction (UAE) method from watermelon seed (WMS). The optimization of various ... CHROMATOGRAPHY; and SUPERCRITICAL FLUID EXTRACTION; to prepare samples for analytical measurement of components. ... properties of ultrasonic extracted proteins were found superior to conventionally extracted proteins.highlightsThe UAE method ...
... their flagship automated sample preparation instrument for Supported Liquid Extraction (SLE),... ... Keywords: Software; Automation; Solid Phase Extraction; Analytic Sample Preparation Methods. Published by B3C newswire and ... "Since its introduction in 2015, the Biotage® Extrahera™ has transformed automated sample preparation with its precision ... their flagship automated sample preparation instrument for Supported Liquid Extraction (SLE), Solid Phase Extraction (SPE), ...
However, even with consistency across sample preparation methods, there may be differential effects on certain analytical ... 3.3.1 When and how should validation assess analytic[al] sensitivity, analytic[al] specificity, accuracy (assay concordance), ... Complex sample preparation and analysis. Fernandez-Leiro and Scheres5. • Nanoparticles. • Gold nanoparticle-tagged antibodies. ... Optimal cryofixation methods vary depending on sample type, and detailed protocols can be found in the book by Wolkers and ...
Plasma preparation and analytic methods. Code-labeled samples were sent to an independent laboratory for the determination of ... Sample size calculations were based on pilot data of mean changes in the incremental area under the curve (iAUC) for glucose ... RESEARCH DESIGN AND METHODS Overweight/obese adults (n = 19), aged 45-65 years, were recruited for a randomized three-period, ... RESEARCH DESIGN AND METHODS. Study overview. This randomized, three-period, three-treatment crossover trial was approved by the ...
Analytic Sample Preparation Methods * Animals * Chromatography, Affinity * Humans * Phosphopeptides / analysis * ... Emphasis will be on the affinity methods utilized specifically for phosphoprotein and phosphopeptide enrichment prior to MS ... phosphoproteomic studies are conducted by mass spectrometric strategies in combination with phospho-specific enrichment methods ... analysis, and on recent applications of these methods in cell biological applications. ...
... method directly measures multiple metals in urine specimens using mass spectrometry after a simple dilution sample preparation ... Please refer to the NHANES Analytic Guidelines and the on-line NHANES Tutorial for further details on the use of sample weights ... Laboratory Method Files. Metals - Urine (August 2021) Laboratory Quality Assurance and Monitoring. Urine samples are processed ... In the MEC, these methods include performing blind split samples collected on "dry run" sessions. In addition, contract ...
Computational and Mathematical Methods in Medicine is a peer-reviewed, Open Access journal that publishes research and review ... separation analysis is that the matrix-dependent alkylation and dealkylation reactions can occur in the sample preparation and ... 2. Materials and Methods. 2.1. Study Subjects and Sample Collection. The subjects of this study were participated from the ... Comparative Screening Analytic Methods for Elderly of Blood Methylmercury Concentration between Two Analytical Institutions. ...
The method for qualitative anti-HD determination is a simultaneous competitive assay. Anti-HD present in the sample and labeled ... Please refer to the NHANES Analytic Guidelines and the on-line NHANES Tutorial for further details on the use of sample weights ... Each laboratory staff member is observed for equipment operation, specimen collection and preparation; testing procedures and ... In the MEC, these methods include performing blind split samples collected on "dry run" sessions. In addition, contract ...
Open Access journal that publishes original research articles as well as review articles reporting methods and instrumentation ... Journal of Analytical Methods in Chemistry is a peer-reviewed, ... Formerly, sample preparation methods were based on a few ... Sensitive analytic methods have been developed to monitor and detect the MRL values in the dairy milk [3]. There are ultra-high ... This method utilized a simple and quick sample preparation procedure using a single extraction step. Through this method, milk ...
METHODS: Samples were analysed with an eubacterial real-time PCR assay that enables detection of bacterial DNA and simultaneous ... PCR analysis in conjunction with MolYsis DNA preparation allows rapid detection of pathogens in blood culture samples. Thus, ... DNA preparation was performed with the new tool MolYsis.. RESULTS: With the Gram-differentiating PCR assay bacteria were ... Rapid detection of pathogens in blood culture bottles by real-time PCR in conjunction with the pre-analytic tool MolYsis.. ...
Kit for Preparation of Technetium Tc99m Sulfur Colloid Injection) may treat, side effects, dosage, drug interactions, warnings ... The random effect meta-analytical methods take into account the sample size of each study as well as within and between study ... Random effect meta-analytic measures were used for estimating various rates of tracer localization by procedure along with the ... The random effect meta-analytical methods take into account the sample size of each study as well as within and between study ...
Sample preparation is one of most important steps in the development of an analytic methodology in herbal preparation. The ... An easy, rapid method of extraction was performed for capsicnoids in Capsicum anuum by Matrix Solid Phase Dispersion (MSPD). ... The first proposed method was applied to the determination of isoflavones in soy germ capsules from four different ... Peak assignment of unknown isoflavones in certain samples was assisted by hydrolysis procedures, migration behavior and UV ...
MATERIALS AND METHODS. Sampling.The samples were obtained from the central Baltic Sea during research cruises onboard the RV ... Bacterioplankton preparation.For FISH and total cell counts, 100-ml portions of water samples were directly transferred to ... Hartmann Analytic GmbH, Braunschweig, Germany) was added to 120-ml Winkler bottles containing the collected samples. After ... Bacterial counting of both DAPI-stained samples and hybridized samples was usually done with a standard deviation of less than ...
Chromatography may be carried out on a small scale for analytic purposes or on a medium-to-large scale for preparation and ... One of the most popular methods for separating proteins is ion exchange chromatography. It separates proteins in a sample based ... Genevacs HCl-resistant EZ-2 evaporator offers reliable sample preparation for neonatal screening ... A protein sample is loaded onto the column in a buffer of low salt and then flushed through the column with a gradient of salt ...
Analytic Sample Preparation Methods. MESH. Animals. MESH. Body Fluids/metabolism. MESH. Calibration. MESH. ... Two mass spectrometry-based methods for the quantitative analysis of free amino acids are described. The first method uses ... Two mass spectrometry-based methods for the quantitative analysis of free amino acids are described. The first method uses ... Amino acid analysis in physiological samples by GC-MS with propyl chloroformate derivatization and iTRAQ-LC-MS/MS. Methods in ...
The methods include the use of a component-binding moiety specific to the component of interest, such as an antibody, to detect ... Methods of detecting a component of interest, such as a protein, in a microfluidic system are provided. ... Sample preparation for parallel chromatography. US7112444 *. 24 Apr 2003. 26 Sep 2006. Wisconsin Alumni Research Foundation. ... which is used to introduce a sample comprising a mixture of components into the system. From protein sample well 102, a sample ...
Having preparation and subjected to acid digestion of sediment samples، 0.5 g of each sample was digested with a mixture of ... Materials and Methods. This analytical study was conducted among 397 employees who were selected by simple random sampling. The ... Finally the concentration of these metals was determined using the contrAA 700 Analytic Jena Atomic absorption spectroscopy. ... After sample preparation the specimens were analyzed using ICP-OES. In the next، according to the results of statistical ...
Sample Preparation and Mass Spectrometry. Before use, all centrifuge tubes and pipette tips were cleaned by sequential soaking ... Statistical Methods. Patient Demographics. Demographic information was collected at interview at the time of patient accrual to ... The internal analytic uncertainty on three replicate runs was typically better than 2%. ... Once samples were dry, concentrated, subboiling distilled nitric acid was added to each sample and refluxed at 200°C for 12 h. ...
The analytes were proved to be stable during all sample storage, preparation and analytic procedures. The method was ... The method required a small amount of sample (100 μL) and minimal sample pretreatment. The limits of detection were 0.03 ng/mL ... Excellent sample clean-up was observed and good linearities (r = 0.9982 for plasma sample and 0.9987 for urine sample) were ... for plasma and urine samples, respectively. This method was successfully applied to the analysis of the biological samples ...
Devise quality control metrics for tissue samples, processing and analytic methods, to ensure high-quality data is generated, ... Address technical challenges in the preparation of kidney biopsies or other sources of tissue. ... Devise quality control metrics for tissue samples, processing and analytic methods, to ensure high-quality standards, and ... Use a current state-of-the-art method or analytic pipeline (a series of complimentary methods) to interrogate human kidney ...
However, the methods differed as concerns other aspects of the sample preparation. This is due to the fundamentally different ... HPLC, validation studies, 4-dimethylantipyrine, suppositories, analytic sample preparation. Suppositories are currently very ... With a novel sample preparation method developed by the authors, 4-dimethylaminoantipyrine was determined in these suppository ... The data presented in this paper reveal that a rapid, efficient and robust sample preparation procedure and HPLC method were ...
The sample preparation steps are usually the most common source of analytic error, whether systematic or random (. Barr et al. ... Most methods for measuring organic chemicals in biologic matrices use a sample preparation step to isolate the target chemical( ... The addition of steps into the sample preparation procedure usually increases the overall imprecision of the method. ... The sample preparation process for inorganic chemicals is typically much simpler than for organic chemicals. In some instances ...
With each of these analytical methods, the increasing cost of the instrument comes with the advantage of decreasing sample ... the operational challenges and costs of various sample preparation strategies will also be discussed. The goal is for clinical ... This consideration requires selecting an instrument and workflow that fits laboratory operations and the requisite analytic ... instruments or more involved and time consuming sample preparation techniques. This webinar will present research on different ...
... gene expression was studied after rigorous standardization of analytic methods and tissue banking. In particular, laser ... Tissue sampling and processing. Tissue sampling and genetic analysis were done according to a clinical protocol approved by the ... preparation, and gene expression analysis. ... The Kaplan-Meier method was used to plot DFS, TTP, and OS, ... Materials and Methods. In vitro Study on Pancreas Cancer Cells. Cell cultures. Cell lines (American Type Culture Collection, ...
In MALDI-TOF-MS, a matrix is introduced into sample preparation to enable soft and efficient ionization of different samples ... avoids preparation of a radiolabeled version of the drug. The authors concluded that that method is rapid and sensitive and ... MALDI-TOF-MS is an analytic tool with advantages including high-speed analysis and sample throughput, high detection ... Triplicate samples were prepared, and 3 averaged spectra were collected for each sample. The IPCs/MeTPP (where PC stands for ...
... for their genetic analysis and less intrusive sample collection methods such as a blood draw for serum or plasma preparation, ... The enhanced analytic sensitivity of COLD-PCR allows clinicians to use smaller amounts of sample ...
Analytic Sample Preparation Methods, Clinical Laboratory Techniques, Molecular Diagnostic Techniques, Genetic Techniques, ... In addition to detailing the methods for sample preparation and data acquisition, we here describe the optical setup for FPALM ... Bi-directional flow maximizes residence time between the monolith and sample, and enables large sample volumes to be processed ... sample preparation, nasopharyngeal aspirate, blood, plasma, high-throughput, sequencing ...
  • The purpose of study was to compare the total mercury and methylmercury measurements techniques and detection levels between analytical institutions in two countries using the same elderly human blood samples. (hindawi.com)
  • A rapid and simple multiclass, ethyl acetate (EtOAc) multiresidue method based on liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) detection was developed for the determination and quantification of 26 veterinary drugs and 187 total pesticide residues in milk. (hindawi.com)
  • Rapid detection of pathogens in blood culture bottles by real-time PCR in conjunction with the pre-analytic tool MolYsis. (nih.gov)
  • Aim of this study is the acceleration of detection and identification of bacteria and fungi in blood cultures by molecular methods before positive signalling in an automated system. (nih.gov)
  • Samples were analysed with an eubacterial real-time PCR assay that enables detection of bacterial DNA and simultaneous differentiation of Gram-positive and Gram-negative bacteria. (nih.gov)
  • PCR analysis in conjunction with MolYsis DNA preparation allows rapid detection of pathogens in blood culture samples. (nih.gov)
  • Detection methods are now based on the chemical and physical properties of the molecules being separated, including, but not limited to color, UV absorbance, size, charge, and hydrophobicity. (news-medical.net)
  • 6. The method of claim 1 , wherein the one or more separated components are bound to or adsorbed to one or more particle set in a binding channel region located downstream of the first detection channel region. (google.ca)
  • For the detection of HA, samples and standard were first diluted in Tris-buffered saline (TBS) containing 4M urea while for the measurement of NA they were diluted in TBS containing 0.01% Zwittergent as these conditions significantly improved the detection sensitivity. (jove.com)
  • The detection efficiency in particle accelerators is very high, as such extremely low sigmas are possible simply by measuring more reference standards and/or counting the unknown sample for longer periods of time. (radiocarbon.com)
  • As good as AMS machines are, simultaneous measurements of the 14C modern standard, sample, and blank cannot be done so small shifts up or down in the detection efficiency of the AMS over the course of the run will affect the accuracy of the result, which at times are outside of the smaller quoted sigma values possible. (radiocarbon.com)
  • Advances in EV sample preparation have not kept pace with advances in downstream analytic techniques such as dPCR and NGS, and the full potential of applying analytical methods for genomics and proteomic profiling to the detection of clinically relevant biomarkers shuttled by circulating EVs has yet to be realized. (molbiol.ru)
  • This includes analytical methods for characterizing active ingredients and contaminants in cannabis, the development of industry laboratory standards, methods of cannabis extraction, as well as cannabis detection for law enforcement purposes. (pittcon.org)
  • With overall enhancement in instrument performances ( sensitivity, detection ability, resolving power, throughput, other performance attributes ), sample preparation techniques had no choice but to keep up with these developments and follow the trend. (silicycle.com)
  • Additionally, analytical methods are included that modify previously used methods to obtain lower detection limits, and/or to improve accuracy and precision. (cdc.gov)
  • The method of determination in almost all of the methods is gas chromatography (GC) with either electron capture detection (ECD) or flame ionization detection (FID). (cdc.gov)
  • One of the reported methods used high performance liquid chromatography (HPLC) in conjunction with ultraviolet absorbance detection. (cdc.gov)
  • The method of determination in all of the methods is GC in conjunction with either flame ionization detection (FID) or mass spectrometric (MS) detection. (cdc.gov)
  • A new integrated extraction and real-time PCR-based system for the detection of group B streptococci in antepartum screening samples enriched in Lim broth was compared to the CDC-recommended culture method. (asm.org)
  • While culture-based methods have historically been the gold standard for demonstrating GBS colonization, several recent studies have demonstrated the utility of PCR-based detection as a sensitive and specific alternative ( 1 , 2 , 4 - 6 , 8 , 9 ). (asm.org)
  • With no user intervention, the system then dispenses the sample into a microfluidic chamber where real-time PCR amplification and detection are performed. (asm.org)
  • Epigenome-wide asso- epigenetic epidemiology is growing rapidly, facilitated by ciation studies usually start with a quality control step, popular microarray platforms like the Infinium 450K and often involving discarding individual probes or entire EPIC chips, which offer broad coverage and precise quan- samples with too many high detection p values (resulting tification of DNA methylation. (deepdyve.com)
  • Samples that have been used for other tests before nucleic acid detection testing are at increased risk of contamination. (health.gov.au)
  • Wherever possible, nucleic acid detection tests should be performed on dedicated samples or on aliquots taken before other tests are performed. (health.gov.au)
  • Where there are specific requirements for the transport and handling of samples for nucleic acid detection, these must be documented and available to referring practitioners and laboratories. (health.gov.au)
  • The method for qualitative anti-HD determination is a simultaneous competitive assay. (cdc.gov)
  • The first proposed method was applied to the determination of isoflavones in soy germ capsules from four different pharmaceutical laboratories. (usp.br)
  • A rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of YZG-331 in mouse plasma was developed. (chemweb.com)
  • A rapid, simple and sensitive method was developed and validated using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for determination of albendazole sulfoxide (ABZOX) in human plasma. (chemweb.com)
  • In this study, an ultra-fast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS) method was developed and validated for the determination of compound 1 , compound 2 and Sal B in rat plasma, respectively. (chemweb.com)
  • The use of a hydrolysis-stable internal standard (IS) allows possible losses during sample preparation to be compensated, thus maximising performance of the determination. (chromsystems.com)
  • This protocol describes enzyme-coupled assays for determination of glucose and ethanol concentrations in a sample of cell-free culture medium. (readbyqxmd.com)
  • Analytical methods for the determination of 2-butoxyethanol, 2-butoxyethanol acetate, and metabolites in biological matrices are shown in Table 6-l. (cdc.gov)
  • Analytical methods specific for the determination of 2-butoxyethanol and 2-butoxyethanol acetate in environmental matrices are shown in Table 6-2. (cdc.gov)
  • This Sample Prep Set allows the quantitative determination of adrenaline (epinephrine), noradrenaline (norepinephrine), dopamine, 3-methoxytyramine, metanephrine, normetanephrine and serotonin in patient urine samples by LC-MS/MS. One typical application for this kit is the analysis of patient samples with suspected catecholamine or serotonin secreting tumours. (chromsystems.com)
  • protein (WMSP) obtained by ultrasound-assisted extraction (UAE) method from watermelon seed (WMS). (bioportfolio.com)
  • Functional properties of ultrasonic extracted proteins were found superior to conventionally extracted proteins.highlightsThe UAE method is more efficient for watermelon seed protein extraction.Impact of extraction parameters on the extraction yield was studied.Protein isolate with enhanced functional properties was obtained.Essential amino acid content was determined. (bioportfolio.com)
  • UPPSALA, Sweden , February 18, 2020 / B3C newswire / -- Biotage AB (Biotage), (NASDAQ OMX Stockholm: BIOT.ST) is pleased to announce the launch of a Good Laboratory Practices (GLP) software upgrade for Biotage® Extrahera™, their flagship automated sample preparation instrument for Supported Liquid Extraction (SLE), Solid Phase Extraction (SPE), Phospholipid Depletion (PLD) and Protein Precipitation (PPT). (pharmiweb.com)
  • Biotage has also added a number of non-GLP benefits to the software including single sample processing, dual mode extraction and barcode management. (pharmiweb.com)
  • Total mercury using gold amalgamation direct mercury analysis method (both) and methylmercury using the dithizone extraction and gas chromatography-electron capture detector (GC-ECD) method (N Lab in Japan) and the cold vapor atomic fluorescence spectrophotometer (CVAFS) method (D Lab in Korea) were measured in 47 subjects who agreed to participate in this study. (hindawi.com)
  • Sample preparation was a simple procedure based on liquid-liquid extraction with ethyl acetate containing 0.1% acetic acid, followed by centrifugation and evaporation of the supernatant. (hindawi.com)
  • Therefore, sample preparation is required, particularly in extraction and cleanup. (hindawi.com)
  • The interest for plants of medicinal and cosmetic application demands methods with efficient extraction. (usp.br)
  • An easy, rapid method of extraction was performed for capsicnoids in Capsicum anuum by Matrix Solid Phase Dispersion (MSPD). (usp.br)
  • Derivatization is carried out directly in aqueous samples, thereby allowing automation of the entire procedure, including addition of reagents, extraction, and injection into the GC-MS. The method delivers the quantification of 26 amino acids. (uni-regensburg.de)
  • The sample preparation consisted of a protein precipitation extraction followed by derivatization with 10 M hydrochloric acid-methanol (10:90, v/v) and determined by liquid chromatography coupled with tandem mass spectrometry. (chemweb.com)
  • Plasma samples were pretreated by liquid-liquid extraction with ethyl acetate. (chemweb.com)
  • It is an excellent alternative to more conventional and tedious methods that combine a first Liquid / Liquid Extraction ( or LLE ) followed by a second Solid Phase Extraction ( or SPE ). (silicycle.com)
  • We sought a DNA extraction method with sufficient sensitivity to detect low copy numbers of viral DNA from small punch samples of NSC. (edu.au)
  • DNA was extracted with commercial and noncommercial DNA extraction methods and quantified on a spectrofluorometer using a PicoGreen dsDNA quantification kit. (edu.au)
  • Serial dilutions of purified viral DNA controls determined the sensitivity of the amplification protocol, and seropositive EBV EDTA-blood amplified by nested PCR (nPCR) validated the DNA extraction methods. (edu.au)
  • We conclude that the QIamp Micro DNA extraction method with the added forensic spin-filter step was suitable for retrospective DNA viral assays from NSC. (edu.au)
  • Abstract In this study, the development and assessment of a modified, efficient, and cost-efficient protocol for mDNA (metagenomic DNA) extraction from contaminated water samples was attempted. (bvsalud.org)
  • The method of extraction influenced the degree of glucose polymerization and the mannose/glucose relationship. (bvsalud.org)
  • We concluded that different fractions and methods of extraction of A. blazei produced differing blood profiles in mice inoculated with the Ehrlich tumor. (bvsalud.org)
  • The BD Max system is a benchtop molecular diagnostic system, which provides fully automated clinical sample preparation, cell lysis, nucleic acid extraction, and mixing of nucleic acid with master mix reagents. (asm.org)
  • Supelco′s Supel™-Select SPE are hydrophilic modified styrene based polymers developed for the solid phase extraction of a broad range of compounds from aqueous samples. (bioanalysis-zone.com)
  • Where nucleic acid extraction is required, nucleic acids should be extracted and purified using standard methods. (health.gov.au)
  • Where possible controls should be included which assess the adequacy of sample collection and extraction. (health.gov.au)
  • Solid phase extraction (SPE) is a widely used method of sample preparation used to separate and enrich purified components from aqueous solution. (news-medical.net)
  • Solid phase extraction (SPE) serves as the front end to numerous analytic modalities, particularly but not limited to ga. (labmanager.com)
  • The authors of the papers used an Effect Directed Analysis (EDA) approach, which is a combination of chromatographic separation with in situ (bio)assays and physico-chemical characterisation to discover and identify bioactive compounds in complex plant samples. (globalplantcouncil.org)
  • Sample preparation is one of most important steps in the development of an analytic methodology in herbal preparation. (usp.br)
  • The FaPEx methodology is based on the same principles than the QuEChERS method, while simplifying it to the extreme. (silicycle.com)
  • Suppositories are important tools for individual therapy, especially in paediatrics, and an instrumental assay method has become necessary for the quality control of dosage units. (ijpsonline.com)
  • The aim of this work was to develop a rapid, effective high-performance liquid chromatography method to assay aminophenazone in extemporaneous suppositories prepared with two different suppository bases, adeps solidus and massa macrogoli. (ijpsonline.com)
  • This consideration requires selecting an instrument and workflow that fits laboratory operations and the requisite analytic specifications of the assay. (labroots.com)
  • QIAGEN N.V., a Netherlands holding company, is the leading global provider of Sample & Assay Technologies that are used to transform biological materials into valuable molecular information. (labroots.com)
  • The BD Max GBS assay exhibited acceptable sensitivity (95%) and specificity (96.7%) compared to those of the culture method in this multisite evaluation. (asm.org)
  • Innovative enzymatic HbA1c assay provides the preferred performance advantages over conventional methods. (sysmex-ap.com)
  • This webinar will present research on different liquid chromatography tandem mass spectrometry testosterone analytical methods that can be performed on low-, mid- and high-sensitivity mass spectrometers. (labroots.com)
  • The enhanced analytic sensitivity of COLD-PCR allows clinicians to use smaller amounts of sample for their genetic analysis and less intrusive sample collection methods such as a blood draw for serum or plasma preparation, urine, fine needle aspirates or bronchial lavage as well as studying mutated DNA in tumors directly. (mdtmag.com)
  • The high sensitivity of the Chromsystems method permits both the total contents of pyridinoline (PYD) and deoxypyridinoline (DPD), as well as the concentration of the free pyridinolines (PYD, DPD) in urine to be determined. (chromsystems.com)
  • In the case of total blood mercury concentration analysis method, it was established to some extent, but a variety of analysis of methyl mercury was still proposed. (hindawi.com)
  • The quantity of enzyme tracer bound to the solid phase and consequently the enzyme activity are inversely proportional to the anti-HD concentration present in samples or controls. (cdc.gov)
  • A protein sample is loaded onto the column in a buffer of low salt and then flushed through the column with a gradient of salt concentration or change in pH. (news-medical.net)
  • Finally the concentration of these metals was determined using the contrAA 700 Analytic Jena Atomic absorption spectroscopy. (magiran.com)
  • The method was linear over a concentration range of 0.25-250 ng/mL. (chemweb.com)
  • Preparation of solutions, calculation of concentration. (vutbr.cz)
  • To receive news and publication updates for Journal of Analytical Methods in Chemistry, enter your email address in the box below. (hindawi.com)
  • Quoted sigmas on radiocarbon dates, unfortunately, cannot take into account "indeterminate errors" such as sample homogeneity, chemistry, and to a lesser extent, detector stability. (radiocarbon.com)
  • Analytical method: LA-ICP-MS, Laboratory of Basic Aspects of Analytical Chemistry at the Faculty of Chemistry, University of Warsaw, PL. *S is only present in the metal, whereas **P indicates the presence of corrosion products in the analysed metal. (micorr.org)
  • However, the problem of species separation analysis is that the matrix-dependent alkylation and dealkylation reactions can occur in the sample preparation and separation stages, leading to misjudgment. (hindawi.com)
  • The choice of media depends on the type of molecule being separated, the means of separation, the purpose and scale of separation, as well as the desired purity of the separated sample. (news-medical.net)
  • This method is commonly used for separation of proteins. (news-medical.net)
  • SEC is often used as an early step in protein purifications, such as before an ion exchange separation and to desalt a sample or exchange buffers. (news-medical.net)
  • 12. The method of claim 1 , comprising electrokinetically flowing the mixture of components through the separation channel region. (google.ca)
  • SPME is a simple, fast and green technique for sample collection and separation. (gerstelus.com)
  • Thin-layer chromatography (TLC) and high performance thin-layer chromatography (HPTLC) are well established, chromatographic separation techniques ideally suited for high-throughput screening of bioactive compounds in crude samples. (globalplantcouncil.org)
  • ProteinWorks Kits simplify and accelerate protein digestion, streamlining and standardizing the traditionally complex pre-analytic workflow for LC-MS protein quantification via the surrogate peptide approach. (bioanalysis-zone.com)
  • The measurements were carried out on a Shimadzu Prominence ultra high-performance liquid chromatography system equipped with a 20 Î l sample loop. (ijpsonline.com)
  • The presented data demonstrate the successful development of a rapid, efficient and robust sample preparation and high-performance liquid chromatography method for the routine quality control of the dosage units of suppositories containing 4-dimethylaminoantipyrine. (ijpsonline.com)
  • Conclusions: A more complete examination of samples that may be mislabeled, contaminated, or have poor performance due to technical problems will improve downstream analyses and replication of findings. (deepdyve.com)
  • See Analytic Notes for information on urine lead data from participants aged 6 years and older. (cdc.gov)
  • The method is sensitive and rapid enough to analyze urine specimens from subjects suspected of being exposed to a number of important toxic elements, or to evaluate environmental or other non-occupational exposure to these same elements (Thomas, 2013). (cdc.gov)
  • The GC-ECD was more sensitive method than CVAFS in methylmercury analytic techniques. (hindawi.com)
  • Sensitive analytic methods have been developed to monitor and detect the MRL values in the dairy milk [ 3 ]. (hindawi.com)
  • By using HPLC-MS/MS, we developed and validated a fast, sensitive and accurate method to simultaneously determine UA and Cr in human saliva. (bioanalysis-zone.com)
  • Routinely achieve reproducible, accurate, and sensitive protein quantification with ProteinWorks, Waters new family of protein bioanalysis sample preparation kits. (bioanalysis-zone.com)
  • Making measurements with any analytical method or instrument requires calibration to ensure the accuracy of the measurement. (kaist.ac.kr)
  • There are two common calibration procedures: using a working curve , and the standard-addition method . (kaist.ac.kr)
  • To study the pharmacokinetics of the above components of pollen of B. campestris in rats, a liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed. (chemweb.com)
  • These results demonstrate that MALDI-TOF-MS is a powerful analytic tool for rapid screening and characterization of phosphonium cations as molecular probes. (snmjournals.org)
  • Therefore, the quality control of this widely applied dosage form with a modern, instrumental analytical method is highly desired. (ijpsonline.com)
  • Moreover, despite evidence that NA also induces protective immunity 4 , the amount of NA in influenza vaccines is not yet standardized due to a lack of appropriate reagents or analytical method 5 . (jove.com)
  • This method directly measures multiple metals in urine specimens using mass spectrometry after a simple dilution sample preparation step. (cdc.gov)
  • G01N1/28 - Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. (google.com)
  • All tissue samples were resected specimens obtained from patients undergoing removal of the malignant part of the stomach or the colon. (indmedica.com)
  • Specimens are to be collected according to principles outlined below, as sample contamination can occur at any stage of specimen collection and processing. (health.gov.au)
  • The procedures used for nucleic acid isolation from the full range of sample types, collection methods (eg patient versus staff collection) and the condition of specimens received by the laboratory must be validated and procedures detailed in the laboratory methods manual. (health.gov.au)
  • The trade-off in these considerations is typically between more advanced, and more expensive, instruments or more involved and time consuming sample preparation techniques. (labroots.com)
  • The combination of gas chromatography-TOF (GC-TOF)-based analysis with automatical deconvolution techniques has not been used for analysis of human tumor samples, thus far, but only on mouse tissues in obesity-related research ( 12 ). (aacrjournals.org)
  • The toolset will synergistically integrate advanced computational methods and visual analytics with data-enabled scientific discovery and innovative experimental techniques to revolutionize our approach to materials science and engineering. (ct-si.org)
  • The use of DNA chips adds novel unique capabilities to the arsenal of modern analytic techniques. (hitechno.ru)
  • To minimise the risk of contamination, nucleic acid amplification techniques have some special sample collection and preparation needs, in addition to the usual requirements for pathology testing. (health.gov.au)
  • A simple integration method was introduced which was based on the distributions of gene expression ratios among different microarray data sets. (biomedcentral.com)
  • Inference for multi-parameter models using conjugate prior distributions: mean and variance of a normal random sample. (ncl.ac.uk)
  • Application to random sample models using conjugate and non-conjugate prior distributions. (ncl.ac.uk)
  • Unless otherwise stated, we refer to measurements made on biologic samples from the parents or the child but not from the fetus. (cdc.gov)
  • Beta Analytic does not report standard deviations of less than +/- 30 BP for single measurements since this can lead to a misinterpretation of the accuracy of the results. (radiocarbon.com)
  • The standard-addition method requires multiple measurements for each sample, but can reduce inaccuracies due to interferences and matrix effects. (kaist.ac.kr)
  • All important steps of metabolic profiling in drug development and molecular medicine are described in great detail, starting from sample preparation, to determining the measurement details of all analytical platforms, and finally, to discussing the corresponding specific steps of data analysis. (nih.gov)
  • Both of these methods require one or more standards of known composition to calibrate the measurement. (kaist.ac.kr)
  • The chief advantage of the working curve method is that it is rapid in that a single set of standards can be used for the measurement of multiple samples. (kaist.ac.kr)
  • to prepare samples for analytical measurement of components. (bvsalud.org)
  • The new methods for measurement of radionuclides in urine are being developed to meet this need for internal contamination and dose assessment. (cdc.gov)
  • The isobaric tagging for relative and absolute quantification (iTRAQ) method employs the labeling of amino acids with isobaric iTRAQ tags. (uni-regensburg.de)
  • We describe a method, filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry-based proteomics. (nih.gov)
  • Today, most phosphoproteomic studies are conducted by mass spectrometric strategies in combination with phospho-specific enrichment methods. (nih.gov)
  • Liquid samples are introduced into the mass spectrometer through the inductively coupled plasma (ICP) ionization source, reduced to small droplets in an argon aerosol via a nebulizer, and then the droplets enter the ICP. (cdc.gov)
  • Two mass spectrometry-based methods for the quantitative analysis of free amino acids are described. (uni-regensburg.de)
  • The first method uses propyl chloroformate/propanol derivatization and gas chromatography-quadrupole mass spectrometry (GC-qMS) analysis in single-ion monitoring mode. (uni-regensburg.de)
  • The tags contain two different cleavable reporter ions, one for the sample and one for the standard, which are detected by fragmentation in a tandem mass spectrometer. (uni-regensburg.de)
  • Given this recommendation, and the often high volume of testing, many clinical laboratories are considering the development of their own in-house mass spectrometry-based testosterone analytical methods. (labroots.com)
  • Pipette 200 µl of the sample/calibrator/ Mass Check ® control into each well of the 96 SPE Well Plate. (chromsystems.com)
  • Creativity and innovation are encouraged to obtain the maximum predictive power or insight through computation, data-centric methods, and theory to achieve the goals of DMREF. (nsf.gov)
  • The method transformed gene expression ratios into the form of a reference data set on a gene by gene basis. (biomedcentral.com)
  • Hierarchical clustering analysis, density and box plots, and mixture scores with correlation coefficients revealed that the two data sets were well intermingled, indicating that the proposed method minimized the experimental bias. (biomedcentral.com)
  • The transformation method was slightly more effective when a data set with strong homogeneity in the same experimental group was used as a reference data set. (biomedcentral.com)
  • Proposed method is simple but useful to combine several data sets from different experimental conditions. (biomedcentral.com)
  • With this method, biologically useful information can be detectable by applying various analytic methods to the combined data set with increased sample size. (biomedcentral.com)
  • Therefore, it is necessary to investigate new methods that can effectively combine microarray data sets which were derived from different experimental environments, while simultaneously minimizing systematic bias. (biomedcentral.com)
  • Sample preparation issues and some of the challenges in data interpretation/handling are also discussed. (edu.au)
  • Polysaccharide anomeric correlations were tentatively assigned for each plant sample based on standard samples and various literature data. (nih.gov)
  • This study was designed to generate the data necessary for 510(k) submission to the FDA, so the study design, reference method, and evaluation criteria were performed as required by the FDA. (asm.org)
  • To improve data-analytic and report-writing skills through group project work. (ncl.ac.uk)
  • 15. The method of claim 14 , further comprising admixing 2,2-dimethyl-1,3-dioxolane-4-methanol and a RSO 2 Y reagent to form the compound of formula (VI), wherein Y is chloride, bromide, or iodide. (google.com.au)
  • Emphasis will be on the affinity methods utilized specifically for phosphoprotein and phosphopeptide enrichment prior to MS analysis, and on recent applications of these methods in cell biological applications. (nih.gov)
  • Urine samples are processed, stored, and shipped to the Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA for analysis. (cdc.gov)
  • Dettmer, Katja , Stevens, Axel Peter , Fagerer, Stephan R , Kaspar, Hannelore und Oefner, Peter J. (2012) Amino acid analysis in physiological samples by GC-MS with propyl chloroformate derivatization and iTRAQ-LC-MS/MS. Methods in molecular biology 828, S. 165-181. (uni-regensburg.de)
  • The role of drug transport and metabolism on gemcitabine cytotoxicity was examined with specific inhibitors, whereas transcription analysis of human equilibrative nucleoside transporter-1 (hENT1), deoxycytidine kinase (dCK), 5′-nucleotidase (5′-NT), cytidine deaminase (CDA), and ribonucleotide reductase subunits M1 and M2 (RRM1 and RRM2) was done by quantitative reverse transcription-PCR in tumor tissue isolated by laser microdissection from surgical or biopsy samples of 102 patients. (aacrjournals.org)
  • Our study shows for the first time that large-scale metabolic profiling using GC-TOF MS is suitable for analysis of fresh frozen human tumor samples, and that there is a consistent and significant change in primary metabolism of ovarian tumors, which can be detected using multivariate statistical approaches. (aacrjournals.org)
  • BETA Analytic's final report includes the individual analysis method, the delivery basis, the material type, and the individual pretreatments applied. (radiocarbon.com)
  • Following alignment, a consensus strategy for variant selection was employed along with computational linkage to a formal tumor clonality analysis based on visualization and quantitative methods. (biomedcentral.com)
  • In flow cytometric MRD analysis leukemia-associated immunophenotypes (LAIPs) are indentified at diagnosis and applied to follow-up samples. (molbiol.ru)
  • Cut your Analysis Costs with this New Sample Prep Technique! (silicycle.com)
  • It's an innovative, simple and fast method for the preparation of samples, a crucial step preceding LC/MS/MS or GC/MS/MS analysis. (silicycle.com)
  • Rather, the intention is to identify well-established methods that are used as the standard methods of analysis. (cdc.gov)
  • Instrumental methods are usually calibrated with standards that are prepared (or purchased) using a non-instrumental analysis. (kaist.ac.kr)
  • The methods of hybridization analysis underlie the diagnostics of genetic, cancerous and infectious diseases. (hitechno.ru)
  • the technology of hybridization analysis gives an opportunity of the multiple use of biochip that decreases expenses upon analyzing samples. (hitechno.ru)
  • With the new potential for chemometric analysis using the 2D NMR fingerprint, this gel-state method may provide the basis for an attractive approach to providing a secondary screen for selecting biomass lines and for optimizing biomass processing and conversion efficiencies. (nih.gov)
  • In our labo-ratory, Atomic Absorption Spectrophotometry has been successfully used for the analysis of zinc in biological samples. (indmedica.com)
  • Furthermore, quality checks that the lack of a standard format makes their analysis diffi- go beyond to catch other types of problematic samples cult to automate. (deepdyve.com)
  • This may be replaced by CHEM 671 Instrumental Methods for Chemical Analysis as one of the allowed Level 600 courses as an elective depending on circumstances. (edu.gh)
  • Thermal motions of the E. coli glucose-galactose binding protein studied using well-sampled, semi-atomistic simulations. (harvard.edu)
  • The chromatographic method was fully validated in accordance with current guidelines. (ijpsonline.com)
  • High-performance liquidchromatography is the most widely used chromatographic method applied for biogenic amines in foods. (bvsalud.org)
  • For many labs, automation provides the biggest return on sample preparation because it's the key time-consumi. (labmanager.com)
  • Titration is similar but requires preparation of a primary standard. (kaist.ac.kr)
  • The attention is paid to manipulation with laboratory equipments together with precise, reproducible and safe fundamental analytical practises, such as preparation of solutions, filtration, titration and weighting. (vutbr.cz)
  • Titration methods include acid-base or complexometric titration. (news-medical.net)
  • Beta Analytic does not accept pharmaceutical samples with "tracer Carbon-14" or any other material containing artificial Carbon-14 to eliminate the risk of cross-contamination. (radiocarbon.com)
  • Such conflicting reports may be attributed to multiple factors such as tissue preparation, different sensitivities of analytical methods, basis of ana-lytic methods and sources of contamination and difficulties in differentiating cancerous and non cancerous tissue. (indmedica.com)
  • To direct appropriate medical treatment to the truly affected, we need a method to rapidly and accurately assess internal contamination for a broad array of radionuclides. (cdc.gov)
  • Methods: Quality checks implemented here include 17 control metrics defined by the manufacturer, a sex check to detect mislabeled sex-discordant samples, and both an identity check for fingerprinting sample donors and a measure of sample contamination based on probes querying high-frequency SNPs. (deepdyve.com)
  • Where patient-collected samples are used for diagnosis, clear instruction shall be provided to the patient to reduce the likelihood of sample contamination. (health.gov.au)
  • New tools and methods to provide an improved understanding of tumor clonal architecture are needed to guide therapy. (biomedcentral.com)
  • Whole exome sequencing (WES) on an Illumina HiSeq 2500 was performed on paired tumor and normal samples from a Multiple Myeloma (MM) patient at presentation, then first and second relapse. (biomedcentral.com)
  • We developed an automated sample preparation protocol based on a robotic platform PAL RTC (CTC Analytics AG, Zwingen Switzerland), which represent a modified Bligh and Dyer method producing samples for both hydrophilic metabolomics using GC-MS and lipidomics using SFC-MS simultaneously. (technologynetworks.com)
  • The new MPS Robotic automates liner exchange, improving analytic results. (gerstelus.com)
  • GERSTEL's new MPS Robotic represents the latest, most versatile in autosampler technology, with advanced software that simplifies sample preparation. (gerstelus.com)
  • However, a suitable integration method has not yet been established. (biomedcentral.com)
  • This approach is more cost effective, enabling a more streamlined method to detect and characterise natural products that are suitable candidates for further investigation as potential new drug molecules. (globalplantcouncil.org)
  • MRD in AML can be based on molecular methods (PCR targeting fusion genes, mutations or expression levels of appropriate genes) or on multicolour flow cytometry. (molbiol.ru)
  • Formerly, sample preparation methods were based on a few compounds or a single class of such drugs. (hindawi.com)
  • The purpose of this chapter is to describe the analytical methods that are available for detecting, and/or measuring, and/or monitoring 2-butoxyethanol, 2-butoxyethanol acetate, their metabolites, and other biomarkers of exposure and effect to these compounds. (cdc.gov)
  • Routine analyses for the glycol ethers in water, including 2-butoxyethanol and its acetate are often not completed because frequently used general-purpose GC/MS methods designed to measure priority pollutants do not readily detect these compounds (Eckel et al. (cdc.gov)
  • Sample preparation methods for air samples are based on the adsorption of the target compounds onto a sorbent such as charcoal (Guest et al. (cdc.gov)
  • A group of scientists from Sechenov University , Russia, and La Trobe University , Australia, have developed a fast and cost-effective method of detecting and identifying bioactive compounds in complex samples such as plant extracts. (globalplantcouncil.org)
  • Thus, the number of compounds that are yet to be explored is so huge that methods that can both screen mixtures and identify the compounds responsible for bioactivity are of greater value. (globalplantcouncil.org)
  • Using this method, scientists examined the properties of bioactive compounds from culinary herbs commonly used in the Mediterranean diet: basil, lavender, rosemary, oregano, sage and thyme. (globalplantcouncil.org)
  • Gas chromatography (GC) is method used to separate samples that contain volatile organic compounds. (news-medical.net)
  • Plasma samples containing YZG-331 and YZG-441 (internal standard, IS) were prepared using a simple protein precipitation by the addition of acetonitrile. (chemweb.com)
  • The plasma samples were extracted by protein precipitation using albendazole sulfoxide-d3 as internal standard (IS). (chemweb.com)
  • Refer to the Laboratory Method Files section for a detailed description of the laboratory methods used. (cdc.gov)
  • To establish a method to simultaneously measure uric acid (UA) and creatinine (Cr) in human saliva. (bioanalysis-zone.com)
  • NHANES uses several methods to monitor the quality of the analyses performed by the contract laboratories. (cdc.gov)
  • RESULTS: Both RT-PCR and MALDI-TOF analyses indicated that the causative agent in both patient samples was L. infantum. (bvsalud.org)
  • Just, Allan C. 2018-06-01 00:00:00 Background: Mislabeled, contaminated or poorly performing samples can threaten power in methylation microarray analyses or even result in spurious associations. (deepdyve.com)
  • All Beta Analytic clients have 24/7 access to results via their secure online archive. (radiocarbon.com)
  • The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. (mayomedicallaboratories.com)
  • One day means results are available 1 day after the sample is received in the laboratory. (mayomedicallaboratories.com)
  • The goal of this three-site investigational study was to compare the results obtained by BDM to those obtained by the CDC-recommended culture procedure, which served as the reference method ( 3 ). (asm.org)
  • The RAND Postdoctoral Training Program in the Study of Aging enables outstanding junior scholars in demographic and aging research to sharpen their analytic skills, learn to communicate research results effectively, and advance their research agenda. (rand.org)
  • Results: Nine hundred forty samples were flagged by at least one control metric and 133 samples from 20 datasets were assigned the wrong sex. (deepdyve.com)
  • The methods of teaching are dependent on teaching process and are described in article 7 of Study and examination order of BUT. (vutbr.cz)
  • In these situations, we measure the level of the chemical in another biologic sample to gauge the internal dose. (cdc.gov)
  • Under the auspices of the Chemical Exposures Workgroup of the NCS, we developed this article as part of a larger white paper ( NCS 2004 ) to provide guidance on which biologic samples may be most useful for characterizing exposures of interest in the NCS. (cdc.gov)
  • While this may produce a very small numerical sigma value, that value is strictly limited to the "determinate errors" associated with counting the 14C modern standard (oxalic acid), unknown sample, and chemical blank (background). (radiocarbon.com)
  • Precipitation: This is a gravimetric method in which the reactants and products of a chemical reaction are used to analyze a substance. (news-medical.net)
  • However, because the quantity of the composite product was limited by the electrochemical method, it is still desirable to synthesize conducting polymer composites with both conducting and ferromagnetic behaviors by a chemical method that can produce larger quantities. (theaic.org)
  • Applicant teams should have documented experience with a current state-of-the-art method that can be used or adapted to interrogate human kidney tissue. (nih.gov)
  • The initial UG3 exploratory phase will be used to demonstrate that the site can interrogate existing tissue samples and small numbers of new biopsies. (nih.gov)
  • samples such as blood or tissue. (labroots.com)
  • This includes talks covering the current state of research into cannabis and derivatives for medical purposes, a look at the social stigma of cannabis and whether this is holding back medical research, as well as a wealth of advances in analytical methods for characterizing cannabis, which will play a role in quality control, regulation and pharmaceutical research. (pittcon.org)
  • This method has been successfully applied to the preclinical dog research of peramivir following intragastric administration. (chemweb.com)
  • The method was successfully applied to the pharmacokinetic studies of YZG-331 in mice. (chemweb.com)
  • The developed method was applied successfully to study the pharmacokinetics of DEH, MET and NOR in rats after oral administration of pollen of B. campestris . (chemweb.com)
  • This method was successfully applied to the bioequivalence study in human plasma samples. (chemweb.com)
  • The validated method was successfully applied to the pharmacokinetic study of compound 1 , compound 2 and Sal B after intravenous administration of 6.0 mg/kg in rats, respectively. (chemweb.com)
  • They successfully applied the method to examine Mediterranean and Australian native culinary herbs. (globalplantcouncil.org)
  • In this webinar, we will discuss the latest advances in methods regarding isolation methods as well as their advantages and disadvantages and justification in their support and the challenges that they encounter. (molbiol.ru)
  • As global requirements for food safety are growing in numbers and complexity, safer, faster and more refined methods are needed to increase efficiency and overall capacity of pesticide residue testing laboratories. (silicycle.com)
  • Vaccine samples were applied onto a PVDF membrane using a slot blot apparatus along with reference standards diluted to various concentrations. (jove.com)
  • A new SBSE method detects leachables even at very low concentrations. (gerstelus.com)
  • Electrochemical methods, electrochemical impedance spectroscopy. (vutbr.cz)
  • 12. Electrochemical methods, potentiometry and voltammetry. (vutbr.cz)
  • Alternating electrochemical methods - electrochemical impedance spectroscopy. (vutbr.cz)
  • Beutler E: Red Cell Metabolism: A Manual of Biochemical Methods. (mayomedicallaboratories.com)
  • The increased awareness of the role of zinc and its interactions in metabolism and disease suggested the need for a reliable method to analyze zinc in biological samples, quickly and efficiently. (indmedica.com)
  • Reagents useful for attaching biomolecules (e.g., proteins, oligonucleotides, and other biomolecules) to a surface, processes of attaching molecules to a surface to form modified surfaces using these reagents, and methods of detecting a target compound using these modified surfaces are disclosed. (google.com.au)
  • If students with health impairments who have similar levels of high school preparation and college academic experiences as their unimpaired peers still systematically perform worse than students without health impairments during the first year of college, then institutional processes and academic demands during the transition to college may disadvantage these students. (jhu.edu)
  • Alternatively, there is also a method available using Sample Clean Up Columns (80600/C). (chromsystems.com)
  • Having preparation and subjected to acid digestion of sediment samples، 0.5 g of each sample was digested with a mixture of nitric acid and super-pure ratio of 4 to 1 perchloric acid. (magiran.com)
  • The analytes were proved to be stable during all sample storage, preparation and analytic procedures. (chemweb.com)
  • The research samples are stable up to 7 days at +4°C. For longer periods of storage, deepfreeze them (below -18°C). (chromsystems.com)
  • It is possible to examine a compound if it is stable enough and can be separated from other substances in a sample. (globalplantcouncil.org)
  • The prepared samples are stable for at least 5 days at +2°C to +8°C in amber coloured vials. (chromsystems.com)