Analytic Sample Preparation Methods: Use of various chemical separation and extraction methods, such as SOLID PHASE EXTRACTION; CHROMATOGRAPHY; and SUPERCRITICAL FLUID EXTRACTION; to prepare samples for analytical measurement of components.Specimen Handling: Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation.Chemistry Techniques, Analytical: Methodologies used for the isolation, identification, detection, and quantitation of chemical substances.Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization: A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.Sensitivity and Specificity: Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)Reproducibility of Results: The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.United StatesFood, Preserved: Food that has been prepared and stored in a way to prevent spoilage.Cooking: The art or practice of preparing food. It includes the preparation of special foods for diets in various diseases.Bisacodyl: A diphenylmethane stimulant laxative used for the treatment of CONSTIPATION and for bowel evacuation. (From Martindale, The Extra Pharmacopoeia, 30th ed, p871)Histocytological Preparation Techniques: Methods of preparing cells or tissues for examination and study of their origin, structure, function, or pathology. The methods include preservation, fixation, sectioning, staining, replica, or other technique to allow for viewing using a microscope.Longitudinal Studies: Studies in which variables relating to an individual or group of individuals are assessed over a period of time.Drug Compounding: The preparation, mixing, and assembling of a drug. (From Remington, The Science and Practice of Pharmacy, 19th ed, p1814)Cohort Studies: Studies in which subsets of a defined population are identified. These groups may or may not be exposed to factors hypothesized to influence the probability of the occurrence of a particular disease or other outcome. Cohorts are defined populations which, as a whole, are followed in an attempt to determine distinguishing subgroup characteristics.Microwaves: That portion of the electromagnetic spectrum from the UHF (ultrahigh frequency) radio waves and extending into the INFRARED RAYS frequencies.Particle Size: Relating to the size of solids.Reference Standards: A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy.Tandem Mass Spectrometry: A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection.Cross-Sectional Studies: Studies in which the presence or absence of disease or other health-related variables are determined in each member of the study population or in a representative sample at one particular time. This contrasts with LONGITUDINAL STUDIES which are followed over a period of time.Mass Spectrometry: An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.

Effects of sample dilution, peroxidase concentration, and chloride ion on the measurement of unbound bilirubin in premature newborns. (1/113)

OBJECTIVES: To assess the effects of sample dilution, peroxidase concentration, and chloride ion (Cl(-)) on plasma unbound bilirubin (B(f)) measurements made using a commercial peroxidase methodology (UB Analyzer) in a study population of ill, premature newborns. DESIGN AND METHODS: B(f) was measured with a UB Analyzer in 74 samples at the standard 42-fold sample dilution and compared with B(f) measured at a 2-fold sample dilution using a FloPro Analyzer. B(f) was measured at two peroxidase concentrations to determine whether the peroxidase steady state B(f) (B(fss)) measurements were significantly less than the equilibrium B(f) (B(feq)), in which case it was necessary to calculate B(feq) from the two B(fss) measurements. B(f) was also measured before and after adding 100 mmol/L Cl(-) to the UB Analyzer assay buffer. RESULTS: B(feq) at the 42-fold dilution was nearly 10-fold less than but it correlated significantly with B(feq) at the 2-fold dilution (mean 8.2+/-5.2 nmol/L versus 73.5+/-70 nmol/L, respectively, p<0.0001; correlation r=0.6). The two UB Analyzer B(fss) measurements were significantly less than B(feq) in 42 of 74 (57%) samples, and Cl(-) increased B(feq) in 66 of 74 (89%) samples by a mean of 82+/-67%. CONCLUSIONS: B(fss) measured by the UB Analyzer at the standard 42-fold sample dilution using assay buffer without Cl(-) and a single peroxidase concentration is significantly less than the B(feq) in undiluted plasma. Accurate B(f) measurements can be made only in minimally diluted serum or plasma.  (+info)

Isolation of polysome-bound mRNA from solid tissues amenable for RT-PCR and profiling experiments. (2/113)

Using cell lines and primary cells, it has been shown that translation control plays a key role regulating gene expression during physiological and pathological conditions. The relevance of this type of regulation in vivo (tissues, organs) remains to be elucidated, due to the lack of an efficient method for polysome-bound fractionation of solid tissue RNA samples. A simple and efficient method is described, in which tissue samples were pulverized in liquid nitrogen and lysed with NP40-lysis buffer in the presence of the RNAse inhibitors RNAsin and vanadyl-ribonucleoside complex. After cell lysis, the cytoplasmic extract was loaded into sucrose gradients, fractionated, and RNA prepared from each fraction. The obtained RNA was reverse transcribed with a low efficiency, a problem that was overcome by purifying polyA+ RNA. Aiming to use small quantities of solid tissue samples (10-20 mg/sample), polyA+ RNA purification was discarded, and the different components were individually screened for a negative effect on reverse transcription. The polysaccharide heparin, which is present as a nonspecific RNAse inhibitor, inhibits reverse transcriptase activity, and must be removed from RNA samples for an efficient reaction. Heparin was successfully removed by precipitation of the RNA with lithium chloride, as demonstrated by the reversal of the inhibition on RT-PCR reactions. In summary, we present a reliable method allowing us to prepare high-quality polysome-bound mRNA from small quantities of liquid-nitrogen-frozen solid tissue samples from both human and mouse origin, amenable for Northern blotting, RT-PCR reactions, and expression profiling analyses.  (+info)

Metabolism, pharmacokinetics, and excretion of a nonpeptidic substance P receptor antagonist, ezlopitant, in normal healthy male volunteers: characterization of polar metabolites by chemical derivatization with dansyl chloride. (3/113)

The excretion, biotransformation, and pharmacokinetics of ezlopitant [(2-benzhydryl-1-aza-bicyclo[2.2.2]oct-3-yl)-(5-isopropyl-2-methoxy-benzyl)-amine ], a substance P receptor antagonist, were investigated in healthy male volunteers after oral administration of a single 200-mg (approximately 93 microCi/subject) dose of [(14)C]ezlopitant. The total recovery of administered radioactive dose was 82.8 +/- 5.1, with 32.0 +/- 4.2% in the urine and 50.8 +/- 1.4% in the feces. Mean observed maximal serum concentrations for ezlopitant and total radioactivity were achieved at approximately 2 h after oral administration; thus, ezlopitant was rapidly absorbed. Ezlopitant was extensively metabolized in humans, since no unchanged drug was detected in urine and feces. The major pathway of ezlopitant in humans was the result of the oxidation of the isopropyl side chain to form the omega-hydroxy and omega-1-hydroxy (M16) metabolites. M16 and omega,omega-1-dihydroxy (1,2-dihydroxy, M12) were identified as the major circulating metabolites accounting for 64.6 and 15.4% of total circulating radioactivity, respectively. In feces, the major metabolite M14 was characterized as the propionic acid metabolite and formed by further oxidation of the omega-hydroxy metabolite. The urinary metabolites were the result of cleaved metabolites caused by oxidative dealkylation of the 2-benzhydryl-1-aza-bicyclo[2.2.2]oct-3-yl moiety. The metabolites (M1A, M1B, and M4), approximately 34% of the total radioactivity in urine, were identified as benzyl amine derivatives. These were polar metabolites that were further characterized using the reaction with dansyl chloride to derivatize the primary amines and phenol moieties to less polar analytes. The other metabolites were the result of O-demethylation, dehydrogenation of the isopropyl group, and oxidation on the quinuclidine moiety.  (+info)

Arsenic determination in marine sediment using ultrasound for sample preparation. (4/113)

This work deals with As determination in marine sediment using ultrasound for sample preparation. It is shown that As can be quantitatively extracted from marine sediment using 20% (v/v) HCl and sonication. The slurry is centrifuged and the analyte is determined in the supernatant by hydride generation atomic absorption spectrometry (HG AAS). A flow injection (FI) system is employed for hydride generation, with 0.5% (m/v) NaBH(4) used as reducdant and a 20% (v/v) HCl used as sample carrier. The limit of quantification is 1.6 microg g(-1) of As, which is based on 800 microl of sample solution and 0.200 g of sample mass in a volume of 50 mL. Certified and non certified marine sediment samples were analyzed; the results were in accordance with the certified or reference values. Speciation analysis by HPLC-ICP-MS showed that As(V) is the only detectable As species present in the supernatant of the centrifuged sample.  (+info)

On-plate digestion using a commercial microfraction collector for nano-HPLC matrix-assisted laser desorption/ionization tandem time-of-flight protein analysis. (5/113)

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Synthesis of bis(amino alcohol)oxalamides and their usage for the preconcentration of trace metals by cloud point extraction. (6/113)

C(2)-Symmetric two bis(amino alcohol)oxalamides (diamidediols) were synthesized and fully characterized. A new method was developed and successfully applied for the simultaneous preconcentration of both trace and toxic metals in water, by using C(2)-symmetric compounds. Under the optimum experimental conditions (i.e. pH = 10.0 +/- 0.2, 2.75 x 10(-3) mol L(-1) N,N'-bis[(1R)-1-ethyl-2-hydroxyethyl]ethanediamide (DAD1), 1.75 x 10(-3) mol L(-1) N,N'-bis[(1S)-1-benzyl-2-hydroxyethyl]-ethanediamide (DAD2), 0.10% w/v octylphenoxy-polyethoxyethanol (Triton X-114)), calibration graphs were linear in the range of 2.5 - 25.0 ng mL(-1) for Cu and Cd, 5.0 - 25.0 ng mL(-1) for Co and Ni. The enrichment factors were 18, 23, 18 and 20 for Cd, Cu, Co and Ni in the case of DAD1, respectively; 20, 22, 17 and 20 for Cd, Cu, Co and Ni in the case of DAD2. The limits of detection for DAD1 were found to be 0.45, 0.50, 1.25 and 0.60 ng mL(-1) for Cd, Cu, Co and Ni, respectively, and for DAD2 were found to be 0.44, 0.25, 0.60 and 1.55 ng mL(-1) for Cd, Cu, Co and Ni, respectively. The developed method was applied to the determination of Cu, Cd, Co and Ni in water samples and certified reference materials with satisfactory results.  (+info)

Triple-phase single-drop microextraction of silver and its determination using graphite-furnace atomic-absorption spectrometry. (7/113)

A new method is described for the determination of silver based on triple-phase microextraction using diethyldithio-carbamate (DDTC) and thioaminophenol. Ag is separated and preconcentrated from the matrix of the sample solution, and finally determined by electrothermal atomic-absorption spectroscopy. The parameters that affect the efficiency were investigated. Under the optimized conditions, a 30-fold preconcentration factor with a detection limit of 0.05 microg L(-1) was achieved. The relative standard deviation was 10% (5 determinations). The developed method was applied to the determination of trace Ag in water samples.  (+info)

Moving known libraries to an addressable array: a site-selective hetero-Michael reaction. (8/113)

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*Inductively coupled plasma mass spectrometry

However, they all require long sample preparation times and destroy the sample. Nondestructive techniques for the forensic ... Elemental Impurities-Procedures, will increase the need for ICP-MS technology, where, previously, other analytic methods have ... For other applications that may involve very viscous samples or samples that have particulate matter, a process known as sample ... MS is considered one of the best techniques for analysis of glass due to the short time for sample preparation and sample, ...

*Graduate Record Examinations

Students who do not use these courses often rely on material from university text books, GRE preparation books, sample tests, ... Critics have also stated that the computer-adaptive method of placing more weight on the first several questions is biased ... and 423 on the analytic, while white test-takers averaged 496, 538, and 564, respectively. The National Association of Test ... ETS provides preparation software called PowerPrep, which contains two practice tests of retired questions, as well as further ...

*DNA microarray

... the relative differences in expression among different spots within a sample and between samples is the preferred method of ... It is critical that information about the sample preparation and handling is discussed, in order to help identify the ... Class discovery analysis: This analytic approach, sometimes called unsupervised classification or knowledge discovery, tries to ... The two samples to be compared (pairwise comparison) are grown/acquired. In this example treated sample (case) and untreated ...

*Total organic carbon

A more common method directly measures TOC in the sample by again acidifying the sample to a pH value of two or less to release ... As many of these biotechnology operations include the preparation of medicines, the U.S. Food and Drug Administration (FDA) ... TOC has been an analytic technique used to measure water quality during the drinking water purification process. TOC in source ... of sample can be handled making the methods less sensitive than chemical oxidation methods capable of digesting as much as 10 ...

*Organogels

Then, various methods used to characterize organogels will be explained. Finally, we will review the use of organogels in ... Souge, J. L. Analytic solutions to Smoluchowski's coagulation equation: a combinatorial inter-pretation. J. Phys. A.: Math. Gen ... The tip is lowered into the sample and a laser reflecting off the cantilever allows for precise measurements to be obtained. In ... Koshima, H.; Matsusaka, W. Yu, H. Preparation and photoreaction of organogels based on ben-zophenone. J. Photochemistry and ...

*History of science

New methods of visualizing the activity of the brain, such as PET scans and CAT scans, began to exert their influence as well, ... Inherent in his analytic approach are the concepts of the phoneme, the morpheme and the root. Medicine: Findings from Neolithic ... He was also the first to recognise that amber was a fossilized resin from pine trees because he had seen samples with trapped ... The Hindus excel in the manufacture of iron, and in the preparations of those ingredients along with which it is fused to ...

*Relationship education

Using meta-analytic methods of current best practices to look across the entire body of published and unpublished evaluation ... 11] Sample Ethical Practices agreement - Sample Ethical Practices agreement. [12] "Helping Veterans Strengthen Their Most ... Based on Florida's 1998 Marriage Preparation and Preservation Act, completion of the four-hour class gives same sex couples a ... PAIRS Classes for Same Sex Couples Florida Marriage Preparation and Preservation Act of 1998 [13] U.S. Department of Health & ...

*Amphetamine

Hence, the latter method may be used on samples that test positive using other methods to help distinguish between the various ... Since the first preparation was reported in 1887, numerous synthetic routes to amphetamine have been developed. The most common ... Cochrane Collaboration reviews are high quality meta-analytic systematic reviews of randomized controlled trials. The ... method 5). Another method is the reaction of phenylacetone with ammonia, producing an imine intermediate that is reduced to the ...

*List of theorems

... analytic number theory) Malgrange preparation theorem (singularity theory) Malgrange-Ehrenpreis theorem (differential equations ... Nyquist-Shannon sampling theorem (information theory) Odd number theorem (physics) Open mapping theorem (complex analysis) Open ... voting methods) Girsanov's theorem (stochastic processes) Glaisher's theorem (number theory) Gleason's theorem (Hilbert space) ... Weierstrass preparation theorem (several complex variables, commutative algebra) Weinberg-Witten theorem (quantum field theory ...

*Modeling and simulation

The focus is general methods that can be applied in various problem domains. M&S Applications solve real world problems by ... Examples are supporting deep water operation of the US Navy or the simulating the surface of neighbored planets in preparation ... 2013). "Simulation Study to Validate Sample Allocation for the National Compensation Survey" (PDF). JSM 2013 - Survey Research ... in order to blend algorithmic and analytic techniques through visualizations available directly to decision makers. A study ...

*Self-efficacy

One method is to provide examples of other people acting on a health promotion behavior and then work with the patient to ... The meta-analytic findings of Stajkovic and Luthens(1998) changed the focus on whether self-efficacy is related to performance ... The results of these scales are then compared to student writing samples. The studies included other variables, such as writing ... or insufficient preparation. A person with a low self-efficacy will attribute the result to poor mathematical ability. See ...

*Hypostatic model of personality

The psycho-molecular method uses specific tasks in order to influence specific neurotransmitter systems. Through the control of ... Nearest-neighbor comparison of two persons in a sample involves comparing a person A with the person B who has the closest ... Saulsman, L. M.; Page, A. C. (2004). "The five-factor model and personality disorder empirical literature: A meta-analytic ... that involves living at a farm where the sole activity is food foraging and preparation; Expressive technique, that involves ...

*Smoking cessation

... with one of the main concerns of cotinine testing being the invasiveness of typical sampling methods. While both measures offer ... Hettema, JE; Hendricks, PS (December 2010). "Motivational interviewing for smoking cessation: a meta-analytic review". Journal ... and preparation stages of change" (PDF). J Consult Clin Psychol. 59 (2): 295-304. doi:10.1037/0022-006X.59.2.295. PMID 2030191 ... Various methods exist which allow a smoker to see the impact of their tobacco use, and the immediate effects of quitting. Using ...

*Reminiscence therapy

Problem focused coping involves the individual in a goal-oriented effort to alter the situation with an analytic approach to ... preparation for death all showed improvements after the therapy was concluded. At a follow up depression, other indicators of ... due to the increased organization of methods and purpose of this therapy. The benefits of reminiscence therapy occurs ... although a few studies have looked at other elderly samples. Reminiscence serves different psychological functions, including ...

*Self-healing material

This method relies on the release of an encapsulated healing agent into the damage zone, and is generally a once off process as ... Accordingly, the healed sample property is compared to that of an undamaged control equipped with self-healing agent fnon- ... Konrad, W., Flues, F., Schmich, F., Speck, T., Speck, O. (2013). "An analytic model of the self-sealing mechanism of the ... CS1 maint: Multiple names: authors list (link) Suryanarayana, C., Rao, K.C. and Kumar, D (2008). "Preparation and ...

*Mindfulness

... methods and adds in newer psychological strategies such as mindfulness and mindfulness meditation. Cognitive methods can ... A meta-analytic review". Journal of Consulting and Clinical Psychology. 78 (2): 169-83. doi:10.1037/a0018555. PMC 2848393 . ... samatha is used as a preparation for vipassanā, pacifying the mind and strengthening the concentration in order to allow the ... for providing more methodological details in reported studies and for the use of larger sample sizes. Mindfulness meditation ...

*Self-enhancement

Campbell, W. K. & Sedikides, Constantine (1999), "Self-threat magnifies the self-serving bias: A meta-analytic integration", ... with poor examination preparation mediating the effect. Ultimately, those who readily prepare themselves for the possibility of ... whereas people high in self-esteem preferentially select augmenting as a method of self-promotion to enhance their perceived ... The strategic use of sample information in generalization and stereotyping", Journal of Personality and Social Psychology, 69 ( ...

*Physical exercise

Szuhany KL, Bugatti M, Otto MW (October 2014). "A meta-analytic review of the effects of exercise on brain-derived neurotrophic ... Reilly T, Ekblom B (June 2005). "The use of recovery methods post-exercise". J. Sports Sci. 23 (6): 619-627. doi:10.1080/ ... To identify which public health strategies are effective, a Cochrane overview of reviews is in preparation. Physical exercise ... For instance, there are deficiencies in methodological planning such as uncontrolled nonrandomized trials, small sample sizes, ...

*Homeopathy

200CK is a 200C preparation made using the Korsakovian method. Fluxion and radionics methods of preparation do not require ... Sample I (July 21, 2008). "Pharmacists urged to 'tell the truth' about homeopathic remedies". The Guardian. London. Malik, IA; ... Sackett, David L. (1979). "Bias in analytic research". Journal of Chronic Diseases. 32 (1-2): 51-63. doi:10.1016/0021-9681(79) ... the method of preparation is different. Bach flower preparations are manufactured in allegedly "gentler" ways such as placing ...

*United States Environmental Protection Agency

Flowback and Produced water ... Chesapeake agrees that an indepth study of toxicity, the development of new analytic methods ... water and sediment sampling instruments used in study of ocean and coastline. One of the major missions of the Bold was to ... and required the preparation of an annual environmental report.[better source needed] President Nixon signed NEPA into law on ... the development of new analytical methods and tracers are not practical given the budget and schedule limitation of the study ...

*Methylphenidate

Prashad M (2001). "Approaches to the Preparation of Enantiomerically Pure (2R,2′R)-(+)-threo-Methylphenidate Hydrochloride" ( ... and suggests methods to analyze their efficacy (119). Over the past two decades, research has progressed from identifying ΔFosB ... A Meta-analytic Review of the Literature". Pediatrics. 111 (1): 179-85. doi:10.1542/peds.111.1.179. PMID 12509574. Abelman, Dor ... an unfair advantage in examinations and that as a result universities may want to discuss making students give urine samples to ...

*General der Nachrichtenaufklärung

There was no other indication in interrogations that this method was met in actual practice. The general method seem to have ... For example, the Army Signal School at Halle had been used by the In 7/IV since November 1943 for the preparation of Army keys ... The recipher consists of two elements: Shifting by means of Chiffranten enciphering: Any figure, chosen from Row A (sample code ... termed Rapid analytic machinery that were time consuming to build and costly, and only worked in specifically defined areas, ...
Sample preparation techniques for isolating contaminants using Light Microscopy, Electron Microscopy, FTIR, and SEM instrumentation are taught in this course.
Forster-Carneiro, T., Isaac, R., Pérez, M. and Schvartz, C. (2012) Anaerobic Digestion: Pretreatments of Substrates, in Biogas Production: Pretreatment Methods in Anaerobic Digestion (ed A. Mudhoo), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9781118404089.ch1 ...
misc{cc1e19e6-aa69-4ac6-9633-aa353bdd90cc, author = {Thuvander, Johan and Arkell, Anders and Krawczyk, Holger and Jönsson, Ann-Sofi}, language = {eng}, note = {Conference Abstract}, pages = {2--10}, series = {[Publication information missing]}, title = {Increasing flux during ultrafiltration using different pretreatment methods}, year = {2014 ...
Four new posters by Biotage R&D and Application Scientists have been presented this week at HPLC 2014, New Orleans, LA, USA. The studies focus on clean-up of biofluids using different sample preparation techniques, and how sample preparation is an important aspect of any analytical method. A number of different approaches are covered that can be used to remove the major endogenous components - particularly proteins and phospholipids - and may impact the quality of HPLC methods and data.. P77 Sample Preparation and Protein: A Comparison of Protein Removal with Various Sample Preparation Techniques using Gel ...
The present invention relates to methods for pretreating biological samples for extraction of nucleic acid therefrom. The present invention employs a combination of at least one protein denaturant with one or more of the following elements to form a reaction mixture for extraction of nucleic acid: (1) at least one aprotic solvent, (2) stepwise heating, and (3) sample dilution.
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
Read this case study and learn how the analysis of hair can be a useful tool in many forensic and clinical applications to establish drug use, trends of use and in the assessment of chronic alcohol consumption. Using the Genevac evaporation programme within these DoA produces, enables the excellent recovery of amphetamine, for example, which is renowned for its volatility.
In the quest for renewable and sustainable energy, lignocellulosic biomass, such as herbaceous plants and hardwoods and softwoods, has been shown to be a promising feedstock for the production of second generation biofuels [1]. Lignocellulosic biomass essentially consists of the polysaccharides cellulose and hemicellulose and the aromatic macromolecule lignin. These compounds are present in the plant cell wall as a three-dimensional network giving the plant structure, stability, and resistance.. Pretreatment of the biomass is necessary in order to overcome this recalcitrance and facilitate degradation of polymeric structures [2]-[4]. In particular, the pretreatment methods aim to improve the conversion efficiency of the plant cell wall polysaccharides into fermentable monosaccharides by reducing the cellulose crystallinity or by simply splitting the carbohydrates and lignin for separate downstream processing technologies.. Various pretreatment methods have been developed for this purpose, ...
Article Characterization of Polymer Carbon Sieves, Graphitized Polymer Carbons and Graphitized Carbon Blacks for Sample Preparation Applications. The use of carbons in sample preparation techniques such as solid phase extraction (SPE) and air samplin...
In order to analyze biological specimens for drugs such as benzodiazepines, it is necessary to perform sample preparation to eliminate matrix interference and ion suppression. Solid-phase extraction is generally the preferred sample preparation technique, in this study Disposable Pipette Extraction (DPX) was utilized. DPX is a fast dispersive solid-phase extraction technique that uses loosely contained sorbent in a disposable pipette tip. The sample is aspirated into the tip where it is actively mixed with the sorbent and forms a suspension. The main advantages of the DPX technology are that the extraction is rapid, minimal solvent waste is generated, and the entire process can be fully automated including introduction of the extract to the chromatographic system. The GERSTEL MPS autosampler performs DPX extractions in approximately 5 minutes using reversed phase (DPX-RP), cation exchange (DPX-CX) or immunoaffinity sorbent material. For determination of target drugs, GC/MS or HPLC/MS/MS are ...
As food supply chains become increasingly global, governments are seeking to monitor a wider range of pesticides than ever before. This requires measuring hundreds of compounds in a number of different analyses, meaning labs need multi residue processes and sample preparation techniques that are both accurate and cost-effective. Chromatography combined with mass spectrometry is ideally suited to such demands, particularly with the advent of faster and more sensitive chromatographic methods and mass specs. Agilent offers both established and new complimentary chemistries to separate targets from matrix, enabling labs to cover familiar matrices but also the more challenging ones. And our highly selective mass specs also provide the opportunity to dilute the matrix away whilst still achieving trace level detection. Combined LC/MS and GC/MS multi residue methods often cover the majority of compounds of interest for a given lab and Agilent sample preparation strategies can produce one extract ...
Hnasko, R.M., Lin, A.V., Stanker, L.H., Bala, K., McGarvey, J.A. 2016. Prion extraction methods: comparison of bead beating, ultrasonic disruption and repeated freeze-thaw methodologies for the recovery of functional renilla-prion fusion protein from bacteria. In: Micic, M., editor. Sample Preparation Techniques for Soil, Plant, and Animal Samples. New York, NY: Humana Press. p. 389-399 ...
The fusion method is an effective sample preparation technique for accurate analysis of a wide variety of powder samples including minerals and ores by XRF spectrometry since it eliminates heterogeneity due to grain size effects and mineralogical composition. In order to take advantage of the fusion method, however, appropriate corrections for errors arisen from fusion processes are required. In this application note the fusion method with improved correction for errors is demonstrated using cop
An improved system for screening a multiple of candidate therapeutic or chemotherapeutic agents for efficacy as to a specific patient, in which a tissue sample from the patient is harvested, cultured and separately exposed to a plurality of treatments and/or therapeutic agents for the purpose of objectively identifying. One particularly important tissue sample preparation technique is the initial preparation of cohesive multicellular particulates of the tissue sample. For assays concerning cancer treatment, a two-stage evaluation is contemplated in which both acute cytotoxic and longer term inhibitory effect of a given anti-cancer agent are investigated. The tissue sample technique of the present invention is also useful in assaying expression and/or secretion of various markers, factors or antigens present on or produced by the cultured cells for diagnostic purposes and for using such expression to monitor the applicability of certain candidate therapeutic or chemotherapeutic agents and the
Miyawakis team has been working on the project since 2011, when they discovered that an aqueous solution of urea was promising for brain tissue optical clearing, but they ran into the tissue damage problem. They have now refined the technique by using a solution which also contains the sugar alcohol sorbitol, as they explain in Nature Neuroscience.. "By combining sorbitol in the right proportion with urea, we could create transparent brains with minimal tissue damage, that can handle both florescent and immunohistochemical labeling techniques, and is even effective in older animals," Miyawaki said.. The ScaleS solution also works as a preservative, keeping samples usable for over a year. It can also be combined with other sample preparation techniques: the team used it with a technique for labelling structures that trigger a response from the immune system and another for fluorescent chemical compounds to produce high-resolution images of Alzheimers plaques, which are not detectable in ...
It is the first course at the under graduate level on microstructural characterization of materials. This course will cover the basic principles and techniques of X-ray diffraction, optical, scanning electron and transmission electron microscopy along with demonstrations of the instrument details and imaging experiments through videos. This course also deals with the sample preparation techniques for the microstructural analysis with practical examples through videos. ...
Histofine® primary antibodies and ancillary reagents are developed by Nichirei Biosciences Inc. These primary antibodies designed to stain human tissue sections are compatible with FFPE tissue sections and used for histopathological analysis. Pretreatment methods and dilutions indicated in this catalog are recommended staining conditions using the Histofine® Simple Stain™ MAX PO (MULTI) detection reagent. For other detection reagents, optimization of staining conditions is recommended.. ...
Tytu : Osmotic concentration of gooseberry fruits - the influence of temperature, time, and pretreatment methods on mass transfer and total polyphenol and organic acid content. ...
Somenath Mitra (Editor), Sample Preparation Techniques in Analytical ChemistryWiley-Interscience | ISBN 0471328456 | 2003 Year | PDF | 3,16 Mb | 488 Pages
A large amount of variation can be seen in each set of match samples, thus not allowing for differentiation between one producer and another. Sample preparation method 2 was devised to improve uniformity of the sample, and reduce variation. Sample preparation method 2 was used to prepare more samples for U1 and L1. U1 was chosen because there was a large variability in the samples. L1 was chosen because the sample were similar to U1. It was hoped using preparation method 2 the U1 samples would have less variation and be distinct from the L1 samples ...
Biotage (STO: BIOT), a leading supplier of tools and technology for analytical and medicinal chemistry, announced the introduction of high-performance, AFFINILUTE MIP (Molecularly Imprinted Polymers) columns for analytical sample preparation
Rabbit Polyclonal C3 antibody [C3], C-term. Validated in WB, ICC/IF, IHC-P, IP, Immunosubtraction. Tested in Human, Mouse. Cited in 3 reference(s)., GeneTex products
C3 antibody [C3], C-term (complement component 3) for ICC/IF, IHC-P, IP, WB, Immunosubtraction. Anti-C3 pAb (GTX101316) is tested in Human, Mouse samples. 100% Ab-Assurance.
One important observation about HCP immunoassays is that they occasionally give dilution-dependent results. Dilution dependence arises because the HCPs in the sample (perhaps one or a few HCPs) do not dilute in parallel with those in the standard (a mixture of 1000s of proteins). Limiting amounts of reagents can come in the form of coat, conjugate antibodies, or even Streptavidin-HRP that are associated with "antigen excess" and non-linear dilution behavior. It is advised to check all reagents to minimize impacts of reagent limitation sample dilution linearity. From a product purity perspective, however, sample dilution non-linearity is not an "assay artifact" but rather could be an important indication that a large amount of a particular HCP has co-purified with product. Phospholipase B-like 2 (PLBL2) was found to co-purify with multiple CHO-derived Mab products. It may be that this is a "common" impurity in Mabs produced across the biotech industry since it was found to have co-purified with ...
Sales, means the sales volume of Sample Preparation System Revenue, means the sales value of Sample Preparation System This report studies sales (consumption) of Sample Preparation System in Global market, especially in United States, China, Europe, Japan, focuses on top players in these regions/countries, with sales, price, revenue and market share for each player in these regions, covering
Find and compare a variety of chromatography sample preparation downloads on environmental xprt on the worlds largest environmental portal. View product and services catalogs, brochures, case studies, company news and more. Contact providers to ...
Everything relating to Sample Preparation System: compilations of facts, news, background knowledge, product information and market trends can be found here in the form of a comprehensive, up-to-date dossier.
Sample preparation makes things faster, easier, increases precision, uses fewer resources, and accurate results, which is popular in food testing labs
NIH Funding Opportunities and Notices in the NIH Guide for Grants and Contracts: Innovations in Cancer Sample Preparation (R21, R33, R21/R33) RFA-CA-07-003. NCI
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Integrated automated sample preparation and assay setup, with manual transfer to the detection unit, for all molecular testing needs ...
Integrated automated sample preparation and assay setup, with manual transfer to the detection unit, for all molecular testing needs ...
The importance of accurate sample preparation techniques cannot be overstated--meticulous sample preparation is essential. Often overlooked, it is the midway point where the analytes from the sample matrix are transformed so they are suitable for analysis. Even the best analytical techniques cannot rectify problems generated by sloppy sample pretreatment.Devoted entirely to teaching and reinforcing these necessary pretreatment steps, Sample Preparation Techniques in Analytical Chemistry addresses diverse aspects of this important measurement step. These include:* State-of-the-art extraction techniques for organic and inorganic analytes* Sample preparation in biological measurements* Sample pretreatment in microscopy* Surface enhancement as a sample preparation tool in Raman and IR spectroscopy* Sample concentration and clean-up methods* Quality control stepsDesigned to serve as a text in an undergraduate or graduate level curriculum, Sample Preparation Techniques in Analytical Chemistry also provides an
Lipids are involved in a host of biochemical and physiological processes in corals. Therefore, changes in lipid composition reflect changes in the ecology, nutrition, and health of corals. As such, accurate lipid extraction, quantification, and identification is critical to obtain comprehensive insight into a corals condition. However, discrepancies exist in sample preparation methodology globally, and it is currently unknown whether these techniques generate analogous results. This study compared the two most common sample preparation techniques for lipid analysis in corals: (1) tissue isolation by air-spraying and (2) crushing the coral in toto. Samples derived from each preparation technique were subsequently analysed to quantify lipids and their constituent classes and fatty acids in four common, scleractinian coral species representing three distinct morphotypes (Acropora millepora, Montipora crassotuberculata, Porites cylindrica, and Pocillopora damicornis). Results revealed substantial amounts
Microalgae have recently drawn attention as a potential resource for biofuels generation. In the short term, anaerobic digestion seems the most viable downstream process. Still, microalgae cell wall restrains the hydrolysis, hampering the methane production potential. This work aimed at improving the anaerobic biodegradability of microalgae through different pretreatment methods: microwave, low temperature and high temperature. For the microwave pretreatment, the specific energy was the main parameter affecting biomass solubilisation, increasing up to 800 % compared to untreated biomass. For the low and high temperature pretreatments, temperature was the most important factor, increasing biomass solubilisation up to 1190 and 2140 % in each case. BMP tests showed an increased methane yield after all pretreatments (40-80 %), with the highest results for the microwave pretreatment. In semi-continuous reactors operated at 15 days HRT, the methane production was increased by 30 and 33 % after ...
Woody biomass is one of the most abundant biomass feedstocks, besides agriculture residuals in the United States. The sustainable harvest residuals and thinnings alone are estimated at about 75 million tons/year accordingly to the DOE biomass report [1]. These forest residuals and thinnings could produce the equivalent of 5 billion gallons of lignocellulosic ethanol annually.. Woody biomass feedstocks include softwood and hardwood. Various pretreatment methods have been developed for pretreating biomass before an enzymatic conversion of pretreated biomass into monomeric sugars for a biofuel or a bioproduct conversion process. An overview on lignocellulosic biomass pretreatment methods well described that most of the pretreating methods worked well on herbaceous biomass and hardwood biomass while not working well with high lignin content softwood biomass [2]. Thus, softwood biomass is the most recalcitrant biomass to pretreat for enzymatic hydrolysis. To utilize the most recalcitrant ...
Solid phase extraction has become a popular tool for analytical method development as a sample preparation technique. Solid phase sorbents can generally extract analytes based on polarity or selectively based on highly specific interactions between the analyte and an immobilized surface molecule. Biomolecular recognition based solid phases utilize antibodies, nucleic acids, and proteins as a secondary affinity layer on a solid phase support for selective analyte extractions and have been used for sample preparation, purification, separations, bioassays, and molecular diagnostics. Polymer supports for solid phase extraction are desirable for their low cost, abundance, chemical inertness, and pH stability. This thesis reports the use of capillary-channel polymer (C-CP) fibers as a solid support modified for selective extraction and on fiber fluorescence detection of nucleic acids and proteins. The polymer fibers are low cost and their unique shape allows them to be easily adapted into a fluidic fiber
Having to reach ever lower limits of detection is a daily challange in modern laboratories. In order to succeed in obtaining sufficiently sensitive analysis methods, sample preparation techniques such as Solid Phase Extraction (SPE) or Liquid-Liquid Extraction are often used as concentration steps. The concentration factor achieved in these cases depends on the amount of solvent used for analyte elution from the SPE cartridge or for liquid extraction. Following the extraction step, further concentration of analytes can be achieved by reducing the amount of solvent left in the extract. This is typically achieved by evaporation. Such a concentration step can contribute significantly to improved limits of detection for the overall analytical method. For the evaporative concentration step, commercially available rotary evaporators as well as custom solutions are widely used. These are mainly stand-alone systems for manual operation. The GERSTEL MultiPosition Evaporation Station (mVAP) in combination ...
There has been little development regarding analysis of polonium (Po) in environmental samples since the 1960ies. This is due to the straightforward spontaneous deposition of this element on silver (Ag), nickel (Ni) or copper (Cu) without any radiochemical separation. For many years, no radiochemical yield determinant was used and it was generally supposed that the yield was 100% after two depositions. Counting was often done using ZnS scintillation counter coupled to a photomultiplier tube. However, the use of the yield determinants 208Po and 209Po and the development of alpha spectrometry showed that the yield was lower. Furthermore, the tendency of Po to volatilize at low temperatures constrains the sample preparation techniques; dry-ashing cannot be used. But during the wet-ashing procedure, there are still some losses. The aim of this study was to evaluate the Po losses during wet-ashing by the use of a double-tracer technique. We have found that the losses were about 30% when open glass ...
In this study various pretreatment methods of sewage sludge inoculum and the statistical process optimization of de-oiled jatropha waste have been reported. Peak hydrogen production rate (HPR) and hydrogen yield (HY) of 0.36 L H2/L-d and 20 mL H2/g Volatile Solid (VS) were obtained when heat shock pretreatment (95 oC, 30 min) was employed. Afterwards, an experimental design was applied to find the optimal conditions for H2 production using heat-pretreated seed culture. The optimal substrate concentration, pH and temperature were determined by using response surface methodology as 205 g/L, 6.53 and 55.1 oC, respectively. Under these circumstances, the highest HPR of 1.36 L H2/L-d was predicted. Verification tests proved the reliability of the statistical approach. As a result of the heat pretreatment and fermentation optimization, a significant (~ 4 folds) increase in HPR was achieved. PCR-DGGE results revealed that Clostridium sp. were majorly present under the optimal conditions.
The three main components of wood, namely, cellulose, hemicellulose, and lignin, can be used in various areas. However, since lignin covalently crosslinks with wood polysaccharides creating networks that is an obstacle for extraction, direct extraction of different wood components in high yield is not an easy matter. One potential approach to overcome such obstacles is to treat the wood with specific enzymes that degrade the networks by specific catalysis. However, the structure of wood is so compact that the penetration of the wood fibers by large enzyme molecules is hindered. Thus, the pretreatment of wood prior to the application of enzymes is necessary, for "opening" the structure.. One pretreatment method that was performed in this thesis is based on kraft pulping, which is a well-established and industrialized technique. For untreated wood, the wood fibers cannot be attacked by the enzymes. A relatively mild pretreatment was sufficient for wood polysaccharides hydrolyzed by a culture ...
Extrusion pretreatment of lignocellulosic biomasses is a physical continuous pretreatment method for subsequent fermentative ethanol production. Twin-screw elements (conveying, kneading and reverse) have different effects on enzymatic digestibility according to their geometries and functions. The first study evaluated the effects of individual functional screw elements on enzymatic digestibility and found that extrusion enhanced enzymatic digestibility under all tested condition. Reverse and kneading screw elements however resulted in lignin re-distribution over the cellulose fibres, blocked pores (shown via SEM) and resulted in less digestible biomass, compared to conveying screw elements, only. Lignin removal via NaOH reversed this effect and the highest digestibility was found for biomass extruded in the presence of reverse screw elements (the most severe conditions tested in this study). Based on these finding a process was developed for separating xylose from lignocellulosic biomass (steam-exploded
Early buyers will receive 10% customization on reports.. Sample preparation is one of the most important operations when it comes to analysis of a sample. This procedure consumes around 60% of the total workload of labs. There are various steps involved in sample preparation, and following these steps is mandatory as the sample preparation process might become complicated if not executed properly with utmost caution. Sample preparation market has highlighted greater adoption of sample preparation instruments that not only simplify the analytical tasks, but also save considerable amount of time. The major applications of electron microscopy & sample preparation are in the life sciences and material sciences domains. Increasing life science applications of electron microscope and R&D expenditure across varied regions around the world have resulted in the overall growth of the electron microscopy and sample preparation market.. Another major factor driving the global electron microscopy & sample ...
La succiniltransferasi del residuo di diidrolipoilisina è un enzima appartenente alla classe delle transferasi, che catalizza la seguente reazione: succinil-CoA + enzima N6-(diidrolipoil)lisina ⇄ CoA + enzima N6-(S-succinildiidrolipoil)lisina Un multimero (24-mer) di questo enzima forma il core del complesso multienzimatico della succinato deidrogenasi; si lega fortemente sia alla ossoglutarato deidrogenasi (numero EC 1.2.4.2), sia alla ossoglutarato deidrogenasi (trasferisce succinile) sia alla diidrolipoil deidrogenasi (numero EC 1.8.1.4). Il gruppo lipoile di questo enzima è succinilato dalla numero EC 1.2.4.2 e lunica direzione catalizzata dalla 2.3.1.61 è quella dove il gruppo succinile è trasferito al coenzima A. ^ a b (EN) 1.2.4.2, in ExplorEnz - The Enzyme Database, IUBMB. ^ (EN) 1.8.1.4, in ExplorEnz - The Enzyme Database, IUBMB. Perham, R.N., Swinging arms and swinging domains in multifunctional enzymes: catalytic machines for multistep reactions, in Annu. Rev. Biochem., vol. ...
Simplify and standardize your food sample handling when preparing dilutions for direct testing or enrichment. Whether you need accurate and rapid sample dilution or quieter homogenizing, Thermo Scientific™ enrichment and preparation solutions are designed to maximize efficiency. ...
The Sample Preparation Laboratories provide wet lab facillities for Sthe SSRL User Program. The labs support final stage sample preparations and straight-forward laboratory manipulations in Biology, Chemistry, Materials Science and the Geosciences. SSRL houses two BioChemMat-focused Laboratories (BCM1 & 2) and one Geoscience-focused Laboratory. All Sample Preparation Laboratories are potentially available to all users.. ...
Selected T1 characteristics of the full sample (n = 1,709), the at-risk sample (n = 1,564), and the analytic sample (n = 1,139) are displayed in Table 1. An equal percentage of respondents in their 40s, 50s, and 60s were recruited at T1. The T1 cohort was predominantly white, married, and employed, had studied beyond high school, and had generally high household incomes. The 1,139 men in the analytic sample were significantly more likely to be younger, white, married, and employed and to have higher education and household incomes (all P ≤ 0.003) than the remaining 425 at-risk men not in the analytic sample.. Table 2 shows the distribution of sleep duration in the at-risk sample, the analytic sample, and those not in the analytic sample. Although there were small differences in the distribution of this variable between those in and not in the analytic sample, the difference was not statistically significant (χ42, P = 0.06).. There were a total of 90 cases of diabetes during 14,737 ...
Selected T1 characteristics of the full sample (n = 1,709), the at-risk sample (n = 1,564), and the analytic sample (n = 1,139) are displayed in Table 1. An equal percentage of respondents in their 40s, 50s, and 60s were recruited at T1. The T1 cohort was predominantly white, married, and employed, had studied beyond high school, and had generally high household incomes. The 1,139 men in the analytic sample were significantly more likely to be younger, white, married, and employed and to have higher education and household incomes (all P ≤ 0.003) than the remaining 425 at-risk men not in the analytic sample.. Table 2 shows the distribution of sleep duration in the at-risk sample, the analytic sample, and those not in the analytic sample. Although there were small differences in the distribution of this variable between those in and not in the analytic sample, the difference was not statistically significant (χ42, P = 0.06).. There were a total of 90 cases of diabetes during 14,737 ...
The murine intestinal tract represents a difficult organ system to study due to its long convoluted tubular structure, narrow diameter, and delicate mucosa which undergoes rapid changes after sampling prior to fixation
The murine intestinal tract represents a difficult organ system to study due to its long convoluted tubular structure, narrow diameter, and delicate mucosa which undergoes rapid changes after sampling prior to fixation
Science Archive. Dec. 22, 2014Science Scanning Probe Microscopy Collagen Surface Functionalization We tested several sample preparation methods for collagen surfaces, suitable for Single Molecule
The present invention relates to a compound of Formula I or a pharmaceutically acceptable salt thereof, its preparation method, a pharmaceutical composition comprising the compound, and its use in manufacture of a medicament for treatment of a disease or disorder, wherein R1, R2, R5, R6, X, Y, Q, W, n1
Eliot Colemans 6-Step Bed Preparation Method - Follow these steps to prepare your beds and ensure vigorous growth. Field-tested tools from Johnnys Selected Seeds.
Sample preparation is often the first step in an analysis; the result of this step can affect the rest of the analytical process. You need a sample that is representative, reproducible, homogenous, and suitable for column injection or other assay. Agilent sample preparation products get your samples ready for analysis, giving you sample aliquots that are compatible with your separation and detection methods. Agilent Bond Elut SPE, Chem Elut, Plexa and Captiva are among the many well trusted names supported by years of usage and peer reviewed studies ...
Described is a system and method for in situ sample preparation and imaging. The system includes a multi-axis stage 100 having a bulk stage 110 and a grid stage 150 with various degrees of freedom to allow for sample preparation. In some embodiments, a focused ion beam system is used to prepare a lamella on the bulk stage 110. The lamella can then be transferred to the grid stage 150 from the bulk stage 110 without needing to move the multi-axis stage 100 from the focused ion beam system.
Updated Parameter (2625) Plasma dilution in Procedure (183) Insulin Blood Level in Pipeline (7) IMPC Pipeline to version 1.2. name changed from Plasma dilution to Sample dilution. minor_version changed from 1 to 2. description changed from plasma_dilution to sample_dilution ...
Course/education, 2018-02-20, Basic Course in SEM and TEM (1 ECTS), This course covers fundamental theory and operational principles as well as specimen preparation techniques for scanning and transmission electron microscopy (SEM/TEM).
From easy Crêpe recipes to masterful Crêpe preparation techniques, find Crêpe ideas by our editors and community in this recipe collection.
From easy Summer Salad recipes to masterful Summer Salad preparation techniques, find Summer Salad ideas by our editors and community in this recipe collection.
Health, ...PHOENIX Ariz. June 23 2011 A new automated sample preparation syst...The fully automated integrated high-throughput system is a key tool ...The platform can prepare nearly 1000 samples in less than 24 hours in...The scientific leadership for the integrated system specification and ...,TGen,,Tecan,and,ASU,Biodesign,Institute,develop,sample,preparation,solution,for,proteomic,research,medicine,medical news today,latest medical news,medical newsletters,current medical news,latest medicine news
The global sample preparation market was valued at US$ 5.7 billion in 2017 and is expected to witness a CAGR of 6.3% over the forecast period (2017â€
Screening blood samples from horses for prohibited drugs requires validated methods and instrumentation to maximize sensitivity, selectivity, and minimize sample handling.
Sample application systems can include an extraction mechanism to remove a sample from sample containers, a sample vessel disposed on a deployment mechanism, where the deployment mechanism is arrange
Advanced Cell Diagnostics RNAscope® technology can be applied to any gene and any species, appropriate sample preparation is essential.
Review tips and techniques on how to get the best out of your IHC experiments with our resident specialists, including sample preparation, immunostaining protocol, suggestions for alternative
Qualitest covers a complete and competitive range of Metallography instruments and consumables for Metallographic Sample Preparation.
For example, during the purge and trap analysis of volatile organics, some components may be left behind in the sorbent trap or at a cold spot in the instrument. So it is a common practice to run a deionized water blank between samples. These blanks are critical in any instrument, where sample components may be left behind only to emerge during the next analysis. Solvent/ Reagent Blank. A solvent blank checks solvents and reagents that are used during sample preparation and analysis. Sometimes, a blank correction or zero setting is done based on the reagent measurement. Good measurement practices (GMPs) refer to the specific techniques in sample preparation and analysis. On the other hand, GLPs are independent of the specific techniques and refer to general practices in the laboratory. An important QC step is to have formally documented GLPs and GMPs that are followed carefully. 10. Components of quality control. Standard operating procedures (SOPs) are written descriptions of procedures of ...
Time of Flight secondary ion mass spectrometry (TOF-SIMS) has been used to explore the distribution of phospholipids in the plasma membrane of Tetrahymena pyriformis during cell division. The dividing cells were freeze-dried prior to analysis followed by line scan and region of interest analysis at various stages of cell division. The results showed no signs of phospholipid domain formation at the junction between the dividing cells. Instead the results showed that the sample preparation technique had a great impact on one of the examined phospholipids, namely phosphatidylcholine (PC). Phosphatidylcholine and 2-aminoethylphosphonolipid (2-AEP) have therefore been evaluated in Tetrahymena cells that have been subjected to different sample preparation techniques: freeze drying ex situ, freeze fracture, and freeze fracture with partial or total freeze drying in situ. The result suggests that freeze drying ex situ causes the celia to collapse and cover the plasma membrane.
Sugarcane bagasse contains cellulose, lignin and hemicellulose, 39-42%, 20-25% and 25-27% respectively. So it is can be used as a sugar source in many processes. Lignin and hemicellulose must be removed before hydrolysis of cellulose. Several different pretreatment approaches have been studied. The purpose of this research is comparison of acid, ozone and combination of ozone-acid as pretreatment methods for improving enzymatic digestibility of sugar cane bagasse in pilot plant scale. Sugar cane Bagasse was washed and pretreated by sulfuric acid (BA) 0.1 %, sodium hydroxide (BSH) 0.1% and (BS) steam was done at pressure 2 bar. Sugar cane bagasse was also treated by sulfuric acid (1, 2, 2.5, and 5%) at 121 º C and 151 º C. Ozone and ozone-acid methods also was used for delignification of bagasse. Particle diameter of bagasse in all pretreatment methods was 3-4 mm. All pretreated bagasse was hydrolyzed by cellulose enzyme complex and beta-gluocosidase at pH 5 and temperature 45ºC. Glucose and xylose
Looking for isotachophoresis? Find out information about isotachophoresis. A variant of electrophoresis in which ionic species move with equal velocity in the presence of an electric field Explanation of isotachophoresis
Solid phase extraction (SPE) is one of the sample preparation methods most widely used by chromatographers, as demonstrated by the numerous published SPE methods found in the literature. Manual SPE cartridge formats can vary from disks through individual cartridges with a range of different volumes to 96-well plates. However, solid phase extraction methods can be tedious and time consuming when performed manually. There is therefore an increasing need for the automation of solid phase extraction methods. A robotic X-Y-Z coordinate autosampler commonly used for sample introduction in GC or HPLC can be used to perform a wide variety of sample preparation techniques using a single instrument and controlling software. The MAESTRO software allows the user to control the automation of solid phase extraction methods. In addition to ease of use and intuitive windows-based programming, the tools to optimize method parameters ensuring efficient sequence creation and maximum sample throughput. The sampler ...
Biomass is a crucial energy resource used for the generation of electricity and transportation fuels. Microalgae exhibit a high content of biocomponents which makes them a potential feedstock for the generation of ecofriendly biofuels. Biofuels derived from microalgae are suitable carbon-neutral replacements for petroleum. Fermentation is the major process for metabolic conversion of microalgal biocompounds into biofuels such as bioethanol and higher alcohols. In this review, we explored the use of all three major biocomponents of microalgal biomass including carbohydrates, proteins, and lipids for maximum biofuel generation. Application of several pretreatment methods for enhancement the bioavailability of substrates (simple sugar, amino acid, and fatty acid) was discussed. This review goes one step further to discuss how to direct these biocomponents for the generation of various biofuels (bioethanol, higher alcohol, and biodiesel) through fermentation and transesterification processes. Such an
A pressure-assisted breathing system comprises a flow generator, a circuit connecting the flow generator to a patients respiratory system and an aerosol generator for emitting aerosol particles into the circuit, wherein the circuit defines a path for the emitted aerosol particles having a change in angle no greater than 15 .
This study evaluated the effects of grinding and chopping with/without microwaving on the health-beneficial components, and antioxidant, anti-inflammation and anti-proliferation capacities of commercial kale and broccoli samples. The availability of indole-3-carbinol (I3C) was evaluated using high performanc
Immunoelectron microscopy is a key technique that bridges the information gap between biochemistry, molecular biology, and ultrastructural studies placing macromolecular functions within a cellular context. In Immunoelectron Microscopy: Methods and Protocols, expert researchers combine the tools of the molecular biologist with those of the microscopist. From the molecular biology toolbox, this volume presents methods for antigen production by protein expression in bacterial cells, methods for epitope tagged protein expression in plant and animal cells allowing protein localization in the absence of protein specific antibodies as well as methods for the production of anti-peptide, monoclonal, and polyclonal antibodies. From the microscopy toolbox, sample preparation methods for cells, plant, and animal tissue are presented. Both cryo-methods, which have the advantage of retaining protein antigenicity at the expense of ultrastructural integrity, as well as chemical fixation methods that maintain ...
A preparation method of an image forming material comprising a support having thereon at least an image forming layer is desclosed, comprising subjecting one side of the support to a treatment to modify the surface of the support and coating an image forming layer on the surface-modified support, wherein the image forming layer contains a resin containing a polar group and an compound containing an isocyanate group and the modified surface of the support exhibiting a surface energy of 45 to 60 dyne/cm, and wherein the image forming material is imagewise exposed to a high density energy light, thereby making an exposed area of the image forming layer removable and removing the removable image forming layer to form an image.
Part Number: 3000002 The Micromixer Chip is a glass microfluidic device designed for millisecond mixing of three fluid streams. Fast mixing times are required for the study of reaction kinetics, sample dilution, improving reaction selectivity, rapid crystallisation and nanoparticle synthesis. The chip can be supplied with a chip holder and a chip header to allow quick connection of fluid pipes. The Micromixer Chip is a static mixer (no moving parts), which at low flow rates creates lamination of the flow streams. The lamination of the streams reduces diffusion distances and hence improves mixing time. At high flow rates swirling occurs in the flow streams, reducing mixing time even further. ...
the Metabolism panel uses a 1:10 dilution of sample, whereas our technical validation assays are performed in vitro using recombinant analytes. Technical validation data reported on the biomarker pages below, and in the data validation document for this panel are based on these in vitro assays. A multiplication factor of 10 should therefore be taken into consideration when comparing the addressable biological concentration to the in vitro validation data. (Download the sample dilution guide document for this panel here). A comprehensive list of all protein biomarkers that can be measured using the currently available biomarker panels can be seen on the biomarker library page.. Note: A printable list of the biomarker assays available in this panel, as well as a full data validation document, can be downloaded from our Document download center ,,. If you prefer not to run the assay yourself, let our experts here at Olink do this for you via our Analysis Service.. The biomarker pages linked to ...
How sensitive is the kit?. The ALLER-TEK™ Gluten ELISA kit is designed with a range of detection from 5-80 ppm. Using dilutions of up to 1,000× with sample dilution buffer, the upper limit of detection can be extended to 80,000 ppm.. Are there limitations to their use?. This assay is designed to accurately quantify gluten content in food and beverage products and is approved as a Performance Tested MethodSM (Cert. #081202). The antibody in this assay recognizes multiple fractions of gluten, including both glutenins and gliadins, and this assay has been calibrated for the quantification of total wheat gluten; the detection of barley gluten can be determined by using our barley standards (Product #510821I).. How long does testing take and how many samples can be tested?. Sample extraction takes as little as 20 minutes with centrifugation or 50 minutes without. The remainder of the assay can be completed in approximately two and a half hours. The assay comes in a 96-well format; in addition to ...
Introduction. Experiment 4 Title : The Determination of Microbial Numbers Objectives: * Practically every phase of microbiology requires method for measuring microbial numbers. * Study the theoretical relationship of one bacterial cell, or clump of cells. * Study the effect of dilution to the bacteria growth. * Determine the cell masses of a culture in order estimates the total cellular protoplasm per milliliter of culture. * To learn both quantitative plating methods which are spread plate and pour plate to measure the number of bacteria. * To understand the measurement for the number bacteria by performing plate and dilution count. Result and Observations: Part I: Spread Plate Unlabelled sample - Dilution factor 10-1 Sample A - Dilution factor 10-2 Sample B - Dilution factor 10-3 Sample C - Dilution factor 10-4 Observation: According to the observation, the result is showed that the colonies of E.coli cultures are too numerous to count via normal visible with density diminish from sample A to ...
sample preparation: In analytical chemistry, the processes in which a representative piece of material is extracted from a larger amount and readied for analysis. Sampling and sample preparation have...
The market study on Global Sample Preparation Market 2017 Research Report studies current as well as future aspects of the Sample Preparation Market primarily based upon factors on which the companies compete in the market, key trends and segmentation analysis. This report covers each side of the worldwide market, ranging from the fundamental market info […]. ...
1.1 This test method covers the determination of total carbon (TC), inorganic carbon (IC), total organic carbon (TOC), dissolved organic carbon (DOC), and non-purgable organic carbon (NPOC) in water, wastewater, and seawater in the range from 0.5 mg/L to 4000 mg/L of carbon. Higher levels may be determined by sample dilution. The sample is injected onto a quartz bed heated at 680ºC. The sample converts into a gaseous phase and forced through a layer of catalyst ensuring conversion of all carbon containing compounds to CO2. A non-dispersive infrared (NDIR) detector measures the resulting CO2. ...
... ,Immuno Concepts manufactures slides that are compatible with multichannel pipettors, which allows the technologist to decrease the number of pipetting steps required to prepare and place the patient sample dilutions on the slides. Ask Immuno Concepts for more information about this procedure and th,medicine,medical supply,medical supplies,medical product
MDI-based prepolymers are blown with a substantially nonaqueous blowing agent, such as pressurized air, and polymerized with stoichiometric amounts of polyoxyethylene polyol having at least two hydroxyl equivalents per mole, yielding a hydrophilic foam. The present foams may be extruded, knife-coated or otherwise cast into sheets, or may be fabricated by other known foam preparation techniques. Because the foam is polymerized with polyoxyethylene polyol instead of water, the foam exhibits both superior drape and improved stretch and recovery as compared with prior-art MDI-based flexible foams formed with aqueous reactants. The foam is particularly suited for use in external biomedical applications as, for example, a laminated medical/surgical dressing in which a thin sheet of the hydrophilic foam adheres to a nonstick aluminized veil on one side.
E D 3 6 & E H D 3 6 DigiBlock Sample Preparation System APPLICATION NOTES LABTECH INC. Your Lab, Our Tech CONTENT 1 ENVIRONMENTAL SOIL WASTE WATER FOOD RICE
Illumina Introduces TruSeq® Stranded mRNA and Total RNA Sequencing Sample Preparation Solutions Delivers Superior Data Quality for Challenging Samples, Unprecedented View of the
This patent search tool allows you not only to search the PCT database of about 2 million International Applications but also the worldwide patent collections. This search facility features: flexible search syntax; automatic word stemming and relevance ranking; as well as graphical results.
This patent search tool allows you not only to search the PCT database of about 2 million International Applications but also the worldwide patent collections. This search facility features: flexible search syntax; automatic word stemming and relevance ranking; as well as graphical results.
Please Note, The ebooks are not always PDF format, you might receive epub/kindle formats after purchase. This is Digital Version of (Ebook) 978-9
The determination of trace residues and contaminants in food has been of growing concern over the past few years. Residual antibacterials in food constitute a risk to human health, especially because they can contribute to the transmission of antibio
Business introduction letter sample templates are useful to work from when drafting your own business letters function serve as map essay, outlining reader main argument points develop taking june 2007 test under simulated lsat conditions.
Essential in biological functions like cell signaling and, when disturbed, a likely cause of disease, lipids have proven to be a vital force in cell biology. In Lipidomics: Methods and Protocols, an i
Supra-Clean SPE C18-S Column, 500 mg/3 ml are recommended for extracting mid-polar to non-polar compounds when analyzing drugs and drug metabolites in biological matrices, trace organic material in water, and toxins in food.
SPE Supra-Clean® Columns C18-S 200 mg/3 mL are recommended for extracting mid-polar to non-polar compounds when analyzing drugs and drug metabolites in biological matrices, trace organic material in water, and toxins in food.
Normal Human & Animal Matrices BioreclamationIVT has been offering a wide range of normal human and animal biological matrices for over twenty years. Our product offering surpasses anything offered in the market today. Our products are not only readily available but customized to your specifications.
Analysis of compounds present in complex matrices is always a challenge, which can be partly overcome by applying various sample preparation techniques prior to detection. Ideally, the extraction techniques should be as selective as possible, to minimize the concentration of interfering substances. In addition, results can be improved by efficient chromatographic separation of the sample components. The elimination of interfering substances is especially important when utilizing mass spectrometry (MS) as a detection technique since they influence the ionization yields. It is also important to optimize ionization methods in order to minimize detection limits.. In the work this thesis is based upon, selective solid phase extraction (SPE) materials, a restricted access material (RAM) and graphitized carbon black (GCB) were employed for clean up and/or pre-concentration of analytes in plasma, urine and agricultural drainage water prior to liquid chromatography/mass spectrometry (LC/MS). Two SPE ...
SY09-03 Phytoestrogens are naturally occurring diphenolic compounds from plants that are structurally similar to the hormone 17β-estradiol. Possible anticarcinogenic effects of these compounds in breast cancer are attributed to their capability to bind to estrogen receptors. However, large-scale epidemiological studies have been limited by difficulties in quantifying phytoestrogen intake. Previous assays for phytoestrogens in biological fluids have analysed for a limited number of analytes and have often suffered from complex, multi-stage sample preparation techniques. Using newly developed GC/MS and LC/MS methods incorporating triply 13C-labelled standards, seven phytoestrogens (daidzein, genistein, glycitein, O-desmethylangolensin (O-DMA), equol, enterodiol, and enterolactone), it is now possible to measure urinary and circulating levels of phytoestrogens more accurately 1,2. In the absence of food composition data (although analyses are ongoing for UK foods) either phytoestrogens in either ...
Journal of Food Quality is a peer-reviewed, Open Access journal that publishes original research articles as well as review articles related to all aspects of food quality characteristics acceptable to consumers. The journal aims to provide a valuable resource for food scientists, nutritionists, food producers, the public health sector, and governmental and non-governmental agencies with an interest in food quality.
Biogas production from organic materials can be used as a renewable vehicle fuel, provide heat and generate electricity and can thereby reduce the greenhouse gas emissions. This thesis focuses on the biogas production based on lignocelluloses. There is an abundant availability of lignocelluloses, constituting 50% of the total biomass worldwide. However, the biomass recalcitrance limits the microbial degradation as well as the biogas production from these types of materials. In the present work different pretreatment methods have been performed in order to decrease the biomass recalcitrance and improve the biogas production. Steam explosion pretreatment, together with the addition of sodium hydroxide and hydrogen peroxide, has been performed on lignocellulosic-rich paper tube residuals. The pretreatment has resulted in methane yields of up to 493 NmL/gVS, which is an increase by 107% compared with untreated material. Furthermore, the use of an organic solvent, N-methylmorpholine-N-oxide (NMMO), ...
A new sensitive, selective, fast and accurate technique for online sample preparation was developed. Microextraction in a packed syringe (MEPS) is a new miniaturised, solid-phase extraction (SPE) technique that can be connected online to GC or LC without any modifications. In MEPS approximately 1 mg of the solid packing material is inserted into a syringe (100-250 ml) as a plug. Sample preparation takes place on the packed bed. The bed can be coated to provide selective and suitable sampling conditions. The new method is very promising. It is very easy to use, fully automated, of low cost and rapid in comparison with previously used methods. The determination of polycyclic hydrocarbons (PAHs) in water was performed using MEPS as sample preparation method online with gas chromatography and mass spectrometry (MEPS-GC-MS). The results from MEPS as sample preparation were compared with other techniques such as stir bar sorptive extraction (SBSE) and solid-phase microextraction (SPME). The method was ...
This research focuses on (1) detection and Identification of microbes using mass spectrometry; (2) development of biological sample preparation methods compatible with mass spectrometry-based proteomic techniques; (3) development of detection and identification approaches of microbes using proteomics, metabolomics, and panomics approaches; and (4) development of bioinformatic algorithms using computational biology principles to enhance the reliable identification and taxonomic classification of microbes at various phylogenetic levels. ECBC is equipped with state-of-the-art instrumentation and facilities that will provide multidisciplinary research projects for recent postdoctoral graduate or other interested scientists.. References. Jabbour RE, et al: "Extracellular protein biomarkers for the characterization of enterohemorrhagic and enteroaggregative Escherichia coli strains." Journal of Microbiological Methods 98: 76-83, 2014. Jabbour RE, et al: Mass spectrometry for microbial proteomics." ...
The present invention provides rigid polyurethane foam comprising thermally expandable particles which are displaced to penetrate a strut of a structure of the polyurethane foam so that the particles are exposed into one or more cells of the polyurethane foam during foaming. Further, the rigid polyurethane foam can comprise hollow thermally expanded particles which are displaced to penetrate a surface of a cell in the polyurethane foam so that the particles are exposed into one or more cells of the polyurethane foam during foaming. The rigid polyurethane of the present invention can have excellent insulating properties.

Improving the enzymatic hydrolysis of larch by coupling water pre-extraction with alkaline hydrogen peroxide post-treatment and...Improving the enzymatic hydrolysis of larch by coupling water pre-extraction with alkaline hydrogen peroxide post-treatment and...

Analytic Sample Preparation Methods. Use of various chemical separation and extraction methods, such as SOLID PHASE EXTRACTION ... An extraction method that separates analytes using a solid phase and a liquid phase. It is used for preparative sample cleanup ... CHROMATOGRAPHY; and SUPERCRITICAL FLUID EXTRACTION; to prepare samples for analytical measurement of components. ...
more infohttps://www.bioportfolio.com/resources/pmarticle/2340149/Improving-the-enzymatic-hydrolysis-of-larch-by-coupling-water-pre-extraction-with.html

Extraction of watermelon seed proteins with enhanced functional properties using ultrasound.Extraction of watermelon seed proteins with enhanced functional properties using ultrasound.

Analytic Sample Preparation Methods. Use of various chemical separation and extraction methods, such as SOLID PHASE EXTRACTION ... protein (WMSP) obtained by ultrasound-assisted extraction (UAE) method from watermelon seed (WMS). The optimization of various ... CHROMATOGRAPHY; and SUPERCRITICAL FLUID EXTRACTION; to prepare samples for analytical measurement of components. ... properties of ultrasonic extracted proteins were found superior to conventionally extracted proteins.highlightsThe UAE method ...
more infohttps://www.bioportfolio.com/resources/pmarticle/2528703/Extraction-of-watermelon-seed-proteins-with-enhanced-functional-properties-using-ultrasound.html

Amino acid analysis in physiological samples by GC-MS with propyl chloroformate derivatization and iTRAQ-LC-MS/MS  -...Amino acid analysis in physiological samples by GC-MS with propyl chloroformate derivatization and iTRAQ-LC-MS/MS -...

Analytic Sample Preparation Methods. MESH. Animals. MESH. Body Fluids/metabolism. MESH. Calibration. MESH. ... Two mass spectrometry-based methods for the quantitative analysis of free amino acids are described. The first method uses ... Two mass spectrometry-based methods for the quantitative analysis of free amino acids are described. The first method uses ... Amino acid analysis in physiological samples by GC-MS with propyl chloroformate derivatization and iTRAQ-LC-MS/MS. Methods in ...
more infohttps://epub.uni-regensburg.de/30576/

Adelaide Research & Scholarship: Comparison of DNA extraction methods from small samples of newborn screening cards suitable...Adelaide Research & Scholarship: Comparison of DNA extraction methods from small samples of newborn screening cards suitable...

Analytic Sample Preparation Methods. ... Comparison of DNA extraction methods from small samples of ... We sought a DNA extraction method with sufficient sensitivity to detect low copy numbers of viral DNA from small punch samples ... The mean DNA yield from this method was 3 ng/μl. The limit of detection was 10 viral genome copies in a 50-μl reaction. EBV ... We conclude that the QIamp Micro DNA extraction method with the added forensic spin-filter step was suitable for retrospective ...
more infohttps://drmc.library.adelaide.edu.au/dspace/handle/2440/63738

Search Articles | University of Toronto LibrariesSearch Articles | University of Toronto Libraries

Analytic Sample Preparation Methods - standards , Blood Platelets - chemistry , Humans , Middle Aged , Vascular Endothelial ... methods , Aged, 80 and over , Adult , Female , Aged , Chemotherapy, Adjuvant , Colorectal Neoplasms - pathology , Liver ... analytic sample preparation methods - standards (2) 2 Filter by. Remove filter. angiogenesis factors (2) 2 ...
more infohttps://query.library.utoronto.ca/index.php/search/q?kw=Author:Schauer,%20Dominic

JoVE | Peer Reviewed Scientific Video Journal - Methods and ProtocolsJoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols

Analytic Sample Preparation Methods, Clinical Laboratory Techniques, Molecular Diagnostic Techniques, Genetic Techniques, ... In addition to detailing the methods for sample preparation and data acquisition, we here describe the optical setup for FPALM ... Bi-directional flow maximizes residence time between the monolith and sample, and enables large sample volumes to be processed ... sample preparation, nasopharyngeal aspirate, blood, plasma, high-throughput, sequencing ...
more infohttps://www.jove.com/visualize/abstract/23840621/guillain-barr-syndrome-influenza-vaccination-antecedent-respiratory

JoVE | Peer Reviewed Scientific Video Journal - Methods and ProtocolsJoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols

Analytic Sample Preparation Methods, Clinical Laboratory Techniques, Molecular Diagnostic Techniques, Genetic Techniques, ... Bi-directional flow maximizes residence time between the monolith and sample, and enables large sample volumes to be processed ... tissue samples that may yield small amounts of degraded or damaged DNA. Here, we describe the preparation of barcoded and ... sample preparation, nasopharyngeal aspirate, blood, plasma, high-throughput, sequencing ...
more infohttps://www.jove.com/visualize/abstract/21072188/the-impact-case-diagnosis-coverage-diagnosis-delays-on-effectiveness

Solution-state 2D NMR of ball-milled plant cell wall gels in DMSO-d(6)/pyridine-d(5).  - PubMed - NCBISolution-state 2D NMR of ball-milled plant cell wall gels in DMSO-d(6)/pyridine-d(5). - PubMed - NCBI

Analytic Sample Preparation Methods/methods*. *Cell Wall/chemistry*. *Dimethyl Sulfoxide/chemistry*. *Gels ... a) Gel-samples of ball-milled cell walls; i) gel-sample (~70 mg) in a 5 mm NMR tube in 1 ml of DMSO-d6; ii) a gel-sample in ... Polysaccharide anomeric correlations were tentatively assigned for each plant sample based on standard samples and various ... Aromatic regions of 2D 13C-1H correlation (HSQC) spectra from cell wall gels and soluble lignins from various samples in DMSO-d ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/20090974?dopt=Abstract

Analytical strategies for phosphoproteomics.  - PubMed - NCBIAnalytical strategies for phosphoproteomics. - PubMed - NCBI

Analytic Sample Preparation Methods. *Animals. *Chromatography, Affinity. *Humans. *Phosphopeptides/analysis. *Phosphopeptides/ ... Emphasis will be on the affinity methods utilized specifically for phosphoprotein and phosphopeptide enrichment prior to MS ... phosphoproteomic studies are conducted by mass spectrometric strategies in combination with phospho-specific enrichment methods ... analysis, and on recent applications of these methods in cell biological applications. ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/19235172?dopt=Abstract

Comparative Screening Analytic Methods for Elderly of Blood Methylmercury Concentration between Two Analytical InstitutionsComparative Screening Analytic Methods for Elderly of Blood Methylmercury Concentration between Two Analytical Institutions

Computational and Mathematical Methods in Medicine is a peer-reviewed, Open Access journal that publishes research and review ... separation analysis is that the matrix-dependent alkylation and dealkylation reactions can occur in the sample preparation and ... 2. Materials and Methods. 2.1. Study Subjects and Sample Collection. The subjects of this study were participated from the ... Comparative Screening Analytic Methods for Elderly of Blood Methylmercury Concentration between Two Analytical Institutions. ...
more infohttps://www.hindawi.com/journals/cmmm/2018/2509413/

Surface Plasmon Resonance | Profiles RNSSurface Plasmon Resonance | Profiles RNS

Analytic Sample Preparation Methods. *Binding, Competitive. *Biuret Reaction. *Calorimetry. *Chemical Fractionation. *Chemical ...
more infohttps://profiles.umassmed.edu/display/128361

Mass Spectrometry | Profiles RNSMass Spectrometry | Profiles RNS

Analytic Sample Preparation Methods. *Binding, Competitive. *Biuret Reaction. *Calorimetry. *Chemical Fractionation. *Chemical ... An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers. ...
more infohttps://profiles.umassmed.edu/display/105916

molbiol.ru -> Beckman Coulter Life...molbiol.ru -> Beckman Coulter Life...

Advances in EV sample preparation have not kept pace with advances in downstream analytic techniques such as dPCR and NGS, and ... In this webinar, we will discuss the latest advances in methods regarding isolation methods as well as their advantages and ... Successful protocols to reduce time and decrease sample variability. - How to integrate sample preparation, instrumentation, ... Proper sample preparation for mass spectrometry-based analysis is essential to obtain reproducible and high-quality results. ...
more infohttp://molbiol.ru/forums/index.php?showtopic=539905&mode=linear

molbiol.ru -> Beckman Coulter Life...molbiol.ru -> Beckman Coulter Life...

Advances in EV sample preparation have not kept pace with advances in downstream analytic techniques such as dPCR and NGS, and ... In this webinar, we will discuss the latest advances in methods regarding isolation methods as well as their advantages and ... Successful protocols to reduce time and decrease sample variability. - How to integrate sample preparation, instrumentation, ... Proper sample preparation for mass spectrometry-based analysis is essential to obtain reproducible and high-quality results. ...
more infohttp://molbiol.ru/forums/index.php?showtopic=539905

Pittcon Conference - Expo Cannabis Testing: An Industry Guide - Pittcon Conference - ExpoPittcon Conference - Expo Cannabis Testing: An Industry Guide - Pittcon Conference - Expo

Thermo Scientific say the method, by cutting out sample preparation time, can provide advantages over SPE or liquid-liquid ... with a total extraction and analytic runtime of 10.4 minutes. ... an automated online sample preparation technique - coupled to ... A team from PerkinElmer tested the method on intact and ground cannabis bud samples. They showed that the method could ... Therefore, it is critical to have sensitive methods for detecting low levels in cannabis samples. Chromatographic methods ...
more infohttps://pittcon.org/cannabis-testing-industry-guide/

Liquid Chromatography Tandem Mass Spectrometry Research into Testosterone Analysis: Considerations of Instrument Performance...Liquid Chromatography Tandem Mass Spectrometry Research into Testosterone Analysis: Considerations of Instrument Performance...

With each of these analytical methods, the increasing cost of the instrument comes with the advantage of decreasing sample ... the operational challenges and costs of various sample preparation strategies will also be discussed. The goal is for clinical ... This consideration requires selecting an instrument and workflow that fits laboratory operations and the requisite analytic ... instruments or more involved and time consuming sample preparation techniques. This webinar will present research on different ...
more infohttps://www.labroots.com/webinar/liquid-chromatography-tandem-mass-spectrometry-research-testosterone-analysis-considerations-instrum

Method of preparing standard aerosol samplesMethod of preparing standard aerosol samples

SUBSTANCE: method of zinc extraction from bottom sediments with an ionic liquid includes preparation of an analytic sample. ... A known method of preparation of standard samples of aerosols in the form of thin organic films, which are obtained by adding ... The method of preparation of standard samples of aerosols from a mixture of fine powder containing trace elements, ... To solve this problem the method of preparation of standard samples of aerosols from a mixture of fine powder containing trace ...
more infohttps://russianpatents.com/patent/252/2525427.html

A guide to integrating immunohistochemistry and chemical imaging  - Chemical Society Reviews (RSC Publishing) DOI:10.1039...A guide to integrating immunohistochemistry and chemical imaging - Chemical Society Reviews (RSC Publishing) DOI:10.1039...

However, even with consistency across sample preparation methods, there may be differential effects on certain analytical ... 3.3.1 When and how should validation assess analytic[al] sensitivity, analytic[al] specificity, accuracy (assay concordance), ... Complex sample preparation and analysis. Fernandez-Leiro and Scheres5. • Nanoparticles. • Gold nanoparticle-tagged antibodies. ... Optimal cryofixation methods vary depending on sample type, and detailed protocols can be found in the book by Wolkers and ...
more infohttps://pubs.rsc.org/en/content/articlehtml/2018/cs/c7cs00610a

Transgenomic Expands COLD-PCR LicenseTransgenomic Expands COLD-PCR License

... for their genetic analysis and less intrusive sample collection methods such as a blood draw for serum or plasma preparation, ... The enhanced analytic sensitivity of COLD-PCR allows clinicians to use smaller amounts of sample ...
more infohttps://www.mdtmag.com/news/2011/03/transgenomic-expands-cold-pcr-license

Bio-analytic silicon chips for the detection of developmentalneurotoxic effects of chemicals and drugs in the context of the...Bio-analytic silicon chips for the detection of developmentalneurotoxic effects of chemicals and drugs in the context of the...

Automation of Sample Preparation for Metabolomic Analysis Using Robotic Platform Poster We developed an automated sample ... which represent a modified Bligh and Dyer method producing samples for both hydrophilic metabolomics using GC-MS and lipidomics ... Bio-analytic silicon chips for the detection of developmentalneurotoxic effects of chemicals and drugs in the context of the ... Bio-analytic silicon chips can be used to investigate stem cell differentiation into neurons and for the in vitro on-line ...
more infohttps://www.technologynetworks.com/tn/posters/bioanalytic-silicon-chips-for-the-detection-of-developmentalneurotoxic-effects-of-chemicals-and-drugs-in-the-context-of-the-european-reach-program-230398

Bio-analytic silicon chips for the detection of developmentalneurotoxic effects of chemicals and drugs in the context of the...Bio-analytic silicon chips for the detection of developmentalneurotoxic effects of chemicals and drugs in the context of the...

Automation of Sample Preparation for Metabolomic Analysis Using Robotic Platform Poster We developed an automated sample ... which represent a modified Bligh and Dyer method producing samples for both hydrophilic metabolomics using GC-MS and lipidomics ... Bio-analytic silicon chips for the detection of developmentalneurotoxic effects of chemicals and drugs in the context of the ... Bio-analytic silicon chips can be used to investigate stem cell differentiation into neurons and for the in vitro on-line ...
more infohttps://www.technologynetworks.com/tn/posters/bioanalytic-silicon-chips-for-the-detection-of-developmentalneurotoxic-effects-of-chemicals-and-drugs-in-the-context-of-the-european-reach-program-230397

Archives of Hygiene SciencesArchives of Hygiene Sciences

Having preparation and subjected to acid digestion of sediment samples، 0.5 g of each sample was digested with a mixture of ... Materials and Methods. This analytical study was conducted among 397 employees who were selected by simple random sampling. The ... Finally the concentration of these metals was determined using the contrAA 700 Analytic Jena Atomic absorption spectroscopy. ... After sample preparation the specimens were analyzed using ICP-OES. In the next، according to the results of statistical ...
more infohttp://magiran.com/magtoc.asp?mgID=6972&number=704

Sodium Chloride | ISHAR OnlineSodium Chloride | ISHAR Online

Analytic Sample Preparation Methods. Butylene Glycols. Central Nervous System Depressants. Forensic Toxicology ... BACKGROUND: Intracutaneous or subcutaneous injection of sterile water is rapidly gaining popularity as a method of pain relief ... Determination of gamma-hydroxybutyrate (GHB) and its precursors in blood and urine samples: a salting-out approach. ...
more infohttp://isharonline.org/tags/sodium-chloride

Search Articles | University of Toronto LibrariesSearch Articles | University of Toronto Libraries

Analytic Sample Preparation Methods , Chemical Fractionation - methods , Simvastatin - isolation & purification , Therapeutic ... BIOLOGICAL SAMPLES , QUANTITATION , STATINS , MASS-SPECTROMETRY , BETA-HYDROXY ACID , MS/MS METHOD , IONIZATION , BIOANALYSIS ... methods , Drug interactions , Methods , Antilipemic agents , Metabolites , Ionization , Pharmacy , Nitriles , Liquid ... 9. Full Text Validated UPLC-MS/MS method for simultaneous determination of simvastatin, simvastatin hydroxy acid and berberine ...
more infohttps://query.library.utoronto.ca/index.php/search/q?kw=SubjectTerms:SIMVASTATIN

Chromatography Media SelectionChromatography Media Selection

Chromatography may be carried out on a small scale for analytic purposes or on a medium-to-large scale for preparation and ... One of the most popular methods for separating proteins is ion exchange chromatography. It separates proteins in a sample based ... Genevacs HCl-resistant EZ-2 evaporator offers reliable sample preparation for neonatal screening ... A protein sample is loaded onto the column in a buffer of low salt and then flushed through the column with a gradient of salt ...
more infohttps://www.news-medical.net/life-sciences/Chromatography-Media-Selection.aspx
  • QIAGEN N.V., a Netherlands holding company, is the leading global provider of Sample & Assay Technologies that are used to transform biological materials into valuable molecular information. (labroots.com)
  • A competitive immunoassay technique is used, which involves pre-incubation of anti-HAV in the sample with HAV antigen in the assay reagent, followed by incubation with a conjugate reagent that contains biotinylated mouse monoclonal anti-HAV antibody and horseradish peroxidase (HRP)-labeled mouse monoclonal anti-HAV antibody. (cdc.gov)
  • Methods of detecting a component of interest, such as a protein, in a microfluidic system are provided. (google.ca)
  • 2. The method of claim 1 , wherein the at least one component of interest comprises a protein and the component-binding moiety comprises a protein-binding moiety. (google.ca)
  • A protein sample is loaded onto the column in a buffer of low salt and then flushed through the column with a gradient of salt concentration or change in pH. (news-medical.net)
  • SEC is often used as an early step in protein purifications, such as before an ion exchange separation and to desalt a sample or exchange buffers. (news-medical.net)
  • Affinity chromatography can be used as a one-step purification method, in some circumstances, achieving a greater than 1000-fold purification of a specific protein. (news-medical.net)
  • Dettmer, Katja , Stevens, Axel Peter , Fagerer, Stephan R , Kaspar, Hannelore und Oefner, Peter J. (2012) Amino acid analysis in physiological samples by GC-MS with propyl chloroformate derivatization and iTRAQ-LC-MS/MS. Methods in molecular biology 828, S. 165-181. (uni-regensburg.de)
  • MRD in AML can be based on molecular methods (PCR targeting fusion genes, mutations or expression levels of appropriate genes) or on multicolour flow cytometry. (molbiol.ru)
  • Vaccine samples were applied onto a PVDF membrane using a slot blot apparatus along with reference standards diluted to various concentrations. (jove.com)
  • 8. The method of claim 6 , wherein the one or more particle set comprise a material selected from the group consisting of: PVDF, polyamide, nylon, and nitrocellulose. (google.ca)
  • Emphasis will be on the affinity methods utilized specifically for phosphoprotein and phosphopeptide enrichment prior to MS analysis, and on recent applications of these methods in cell biological applications. (nih.gov)
  • While ion exchange, size exclusion and affinity chromatography media are some of the most popular and commonly used options, there are dozens of other methods. (news-medical.net)
  • Each chapter in the LPM specifies the procedure to be used for preparation, labeling, processing, preservation, and transport of the specimens. (cdc.gov)
  • The sample is loaded into the top of the column and is flushed through with the mobile phase liquid, gravity or under pressure. (news-medical.net)
  • Samples to be analyzed are introduced into this central channel, usually as a mist of liquid formed by passing the liquid sample into a nebulizer. (wikipedia.org)
  • To maximise plasma temperature (and hence ionisation efficiency) and stability, the sample should be introduced through the central tube with as little liquid (solvent load) as possible, and with consistent droplet sizes. (wikipedia.org)
  • A nebuliser can be used for liquid samples, followed by a spray chamber to remove larger droplets, or a desolvating nebuliser can be used to evaporate most of the solvent before it reaches the torch. (wikipedia.org)
  • Here, the profiling method has been improved by using a mixture of perdeuterated DMSO and pyridine (4 : 1, v/v). Adding pyridine provided not only easier sample handling because of the better mobility compared to the DMSO-d(6)-only system but also considerably elevated intensities and improved resolution of the NMR spectra due to the enhanced swelling of the cell walls. (nih.gov)
  • SUBSTANCE: method of preparing standard aerosol samples based on a mixture of fine powder containing defined elements is characterised by that a dispersed mixture of mineral, synthetic and biological materials is used, wherein grain size analysis is used to detect presence of said types of simulating materials and content thereof in a real atmospheric suspension in said region as applied to a specific season is determined. (russianpatents.com)
  • The closest in technical essence and DOS is haemosu result to the invention is a method for preparing standard samples of aerosols on the basis of a mixture of finely dispersed powder, contains trace elements (see EN No. 2239170, G01N 1/28, 2002). (russianpatents.com)
  • Also the disadvantage of this method is the length (about 3 hours) and intensity (all operations are performed manually process a homogeneous mixture of polymer solution and defined components. (russianpatents.com)
  • Having preparation and subjected to acid digestion of sediment samples، 0.5 g of each sample was digested with a mixture of nitric acid and super-pure ratio of 4 to 1 perchloric acid. (magiran.com)
  • 12. The method of claim 1 , comprising electrokinetically flowing the mixture of components through the separation channel region. (google.ca)
  • The method we describe here to produce these molecules is straight forward and can facilitate research in influenza laboratories, since it allows for production of large amounts of proteins fast and at a low cost. (jove.com)
  • If a negatively charged resin is used to capture positively charged molecules, the method is referred to as cation exchange chromatography. (news-medical.net)
  • The sample enters the central channel of the ICP, evaporates, molecules break apart, and then the constituent atoms ionise. (wikipedia.org)
  • Participants aged 3 years and older who do not meet any of the exclusion criteria were sampled. (cdc.gov)
  • With the new potential for chemometric analysis using the 2D NMR fingerprint, this gel-state method may provide the basis for an attractive approach to providing a secondary screen for selecting biomass lines and for optimizing biomass processing and conversion efficiencies. (nih.gov)
  • In the case of total blood mercury concentration analysis method, it was established to some extent, but a variety of analysis of methyl mercury was still proposed. (hindawi.com)
  • In flow cytometric MRD analysis leukemia-associated immunophenotypes (LAIPs) are indentified at diagnosis and applied to follow-up samples. (molbiol.ru)
  • The difference of the contents of the designated elements in the individual instances of the standard samples reduces the accuracy of control of the chemical composition of atmospheric aerosols, which is loaded on the filter, using a variety of spectral and chemical methods of analysis. (russianpatents.com)
  • Our study shows for the first time that large-scale metabolic profiling using GC-TOF MS is suitable for analysis of fresh frozen human tumor samples, and that there is a consistent and significant change in primary metabolism of ovarian tumors, which can be detected using multivariate statistical approaches. (aacrjournals.org)
  • This includes talks covering the current state of research into cannabis and derivatives for medical purposes, a look at the social stigma of cannabis and whether this is holding back medical research, as well as a wealth of advances in analytical methods for characterizing cannabis, which will play a role in quality control, regulation and pharmaceutical research. (pittcon.org)
  • NHANES uses several methods to monitor the quality of the analyses performed by the contract laboratories. (cdc.gov)
  • The methods include the use of a component-binding moiety specific to the component of interest, such as an antibody, to detect the component of interest. (google.ca)
  • However, these methods are generally found to be time-consuming and require large volumes of organic solvents. (hindawi.com)
  • By testing a nationally representative sample of the U.S. population, NHANES will provide the most reliable estimates of age-specific prevalence needed to evaluate the effectiveness of the strategies to prevent these infections. (cdc.gov)
  • 7. The method of claim 6 , wherein the one or more particle set comprises a polymeric material, a silica material, a ceramic material, a glass material, a magnetic material, a metallic material, or an organic material. (google.ca)
  • 9. The method of claim 7 , wherein particles in the particle set are from about 0.1 mm to about 50 mm in diameter. (google.ca)
  • 10. The method of claim 6 , wherein the one or more particle set comprises the component-binding moiety. (google.ca)
  • Bio-analytic silicon chips can be used to investigate stem cell differentiation into neurons and for the in vitro on-line monitoring of cellular reactions under well controlled experimental conditions. (technologynetworks.com)
  • The disadvantage of this approach is a rejection of approximately 5% of instances, with instability on the chemical composition of the remaining individual instances of the standard samples is characterized by a coefficient of variation equal to 4-7% depending on the element being defined. (russianpatents.com)
  • In size exclusion chromatography (SEC), the sample is filtered through a gel made of spherical beads with specifically-sized pores. (news-medical.net)