Amyloidogenic proteins. Medical search

Proteins that form the core of amyloid fibrils. For example, the core of amyloid A is formed from amyloid A protein, also known as serum amyloid A protein or SAA protein.

A fibrous protein complex that consists of proteins folded into a specific cross beta-pleated sheet structure. This fibrillar structure has been found as an alternative folding pattern for a variety of functional proteins. Deposits of amyloid in the form of AMYLOID PLAQUES are associated with a variety of degenerative diseases. The amyloid structure has also been found in a number of functional proteins that are unrelated to disease.

A group of sporadic, familial and/or inherited, degenerative, and infectious disease processes, linked by the common theme of abnormal protein folding and deposition of AMYLOID. As the amyloid deposits enlarge they displace normal tissue structures, causing disruption of function. Various signs and symptoms depend on the location and size of the deposits.

A tetrameric protein, molecular weight between 50,000 and 70,000, consisting of 4 equal chains, and migrating on electrophoresis in 3 fractions more mobile than serum albumin. Its concentration ranges from 7 to 33 per cent in the serum, but levels decrease in liver disease.

A pancreatic beta-cell hormone that is co-secreted with INSULIN. It displays an anorectic effect on nutrient metabolism by inhibiting gastric acid secretion, gastric emptying and postprandial GLUCAGON secretion. Islet amyloid polypeptide can fold into AMYLOID FIBRILS that have been found as a major constituent of pancreatic AMYLOID DEPOSITS.

An acid dye used in testing for hydrochloric acid in gastric contents. It is also used histologically to test for AMYLOIDOSIS.

Peptides generated from AMYLOID BETA-PEPTIDES PRECURSOR. An amyloid fibrillar form of these peptides is the major component of amyloid plaques found in individuals with Alzheimer's disease and in aged individuals with trisomy 21 (DOWN SYNDROME). The peptide is found predominantly in the nervous system, but there have been reports of its presence in non-neural tissue.

Processes involved in the formation of TERTIARY PROTEIN STRUCTURE.

An 11-kDa protein associated with the outer membrane of many cells including lymphocytes. It is the small subunit of the MHC class I molecule. Association with beta 2-microglobulin is generally required for the transport of class I heavy chains from the endoplasmic reticulum to the cell surface. Beta 2-microglobulin is present in small amounts in serum, csf, and urine of normal people, and to a much greater degree in the urine and plasma of patients with tubular proteinemia, renal failure, or kidney transplants.

Small proteinaceous infectious particles which resist inactivation by procedures that modify NUCLEIC ACIDS and contain an abnormal isoform of a cellular protein which is a major and necessary component. The abnormal (scrapie) isoform is PrPSc (PRPSC PROTEINS) and the cellular isoform PrPC (PRPC PROTEINS). The primary amino acid sequence of the two isoforms is identical. Human diseases caused by prions include CREUTZFELDT-JAKOB SYNDROME; GERSTMANN-STRAUSSLER SYNDROME; and INSOMNIA, FATAL FAMILIAL.

The level of protein structure in which regular hydrogen-bond interactions within contiguous stretches of polypeptide chain give rise to alpha helices, beta strands (which align to form beta sheets) or other types of coils. This is the first folding level of protein conformation.

A synuclein that is a major component of LEWY BODIES that plays a role in neurodegeneration and neuroprotection.

The characteristic 3-dimensional shape and arrangement of multimeric proteins (aggregates of more than one polypeptide chain).

Polypeptide chains, consisting of 211 to 217 amino acid residues and having a molecular weight of approximately 22 kDa. There are two major types of light chains, kappa and lambda. Two Ig light chains and two Ig heavy chains (IMMUNOGLOBULIN HEAVY CHAINS) make one immunoglobulin molecule.

A change from planar to elliptic polarization when an initially plane-polarized light wave traverses an optically active medium. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

The assembly of the QUATERNARY PROTEIN STRUCTURE of multimeric proteins (MULTIPROTEIN COMPLEXES) from their composite PROTEIN SUBUNITS.

The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).

Hereditary and sporadic conditions which are characterized by progressive nervous system dysfunction. These disorders are often associated with atrophy of the affected central or peripheral nervous system structures.

Electron microscopy in which the ELECTRONS or their reaction products that pass down through the specimen are imaged below the plane of the specimen.

Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.

A degenerative disease of the BRAIN characterized by the insidious onset of DEMENTIA. Impairment of MEMORY, judgment, attention span, and problem solving skills are followed by severe APRAXIAS and a global loss of cognitive abilities. The condition primarily occurs after age 60, and is marked pathologically by severe cortical atrophy and the triad of SENILE PLAQUES; NEUROFIBRILLARY TANGLES; and NEUROPIL THREADS. (From Adams et al., Principles of Neurology, 6th ed, pp1049-57)

A single-pass type I membrane protein. It is cleaved by AMYLOID PRECURSOR PROTEIN SECRETASES to produce peptides of varying amino acid lengths. A 39-42 amino acid peptide, AMYLOID BETA-PEPTIDES is a principal component of the extracellular amyloid in SENILE PLAQUES.

Diseases in which there is a familial pattern of AMYLOIDOSIS.

A spectroscopic technique in which a range of wavelengths is presented simultaneously with an interferometer and the spectrum is mathematically derived from the pattern thus obtained.

Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.

The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.

Endopeptidases that are specific for AMYLOID PROTEIN PRECURSOR. Three secretase subtypes referred to as alpha, beta, and gamma have been identified based upon the region of amyloid protein precursor they cleave.

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)

Disorders of the peripheral nervous system associated with the deposition of AMYLOID in nerve tissue. Familial, primary (nonfamilial), and secondary forms have been described. Some familial subtypes demonstrate an autosomal dominant pattern of inheritance. Clinical manifestations include sensory loss, mild weakness, autonomic dysfunction, and CARPAL TUNNEL SYNDROME. (Adams et al., Principles of Neurology, 6th ed, p1349)

The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.

The rate dynamics in chemical or physical systems.

A sub-subclass of endopeptidases that depend on an ASPARTIC ACID residue for their activity.

Accumulations of extracellularly deposited AMYLOID FIBRILS within tissues.

Inherited disorders of the peripheral nervous system associated with the deposition of AMYLOID in nerve tissue. The different clinical types based on symptoms correspond to the presence of a variety of mutations in several different proteins including transthyretin (PREALBUMIN); APOLIPOPROTEIN A-I; and GELSOLIN.

A type of scanning probe microscopy in which a probe systematically rides across the surface of a sample being scanned in a raster pattern. The vertical position is recorded as a spring attached to the probe rises and falls in response to peaks and valleys on the surface. These deflections produce a topographic map of the sample.

An abnormal protein with unusual thermosolubility characteristics that is found in the urine of patients with MULTIPLE MYELOMA.

An enzyme the catalyzes the degradation of insulin, glucagon and other polypeptides. It is inhibited by bacitracin, chelating agents EDTA and 1,10-phenanthroline, and by thiol-blocking reagents such as N-ethylmaleimide, but not phosphoramidon. (Eur J Biochem 1994;223:1-5) EC 3.4.24.56.

The delicate interlacing threads, formed by aggregations of neurofilaments and neurotubules, coursing through the CYTOPLASM of the body of a NEURON and extending from one DENDRITE into another or into the AXON.

The thermodynamic interaction between a substance and WATER.

Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.

A basic enzyme that is present in saliva, tears, egg white, and many animal fluids. It functions as an antibacterial agent. The enzyme catalyzes the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. EC 3.2.1.17.

The ability of a protein to retain its structural conformation or its activity when subjected to physical or chemical manipulations.

An ACUTE PHASE REACTION protein present in low concentrations in normal sera, but found at higher concentrations in sera of older persons and in patients with AMYLOIDOSIS. It is the circulating precusor of amyloid A protein, which is found deposited in AA type AMYLOID FIBRILS.

A low-energy attractive force between hydrogen and another element. It plays a major role in determining the properties of water, proteins, and other compounds.

A subclass of PEPTIDE HYDROLASES that catalyze the internal cleavage of PEPTIDES or PROTEINS.

Disruption of the non-covalent bonds and/or disulfide bonds responsible for maintaining the three-dimensional shape and activity of the native protein.

The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.

Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.

Proteins that are involved in the peptide chain termination reaction (PEPTIDE CHAIN TERMINATION, TRANSLATIONAL) on RIBOSOMES. They include codon-specific class-I release factors, which recognize stop signals (TERMINATOR CODON) in the MESSENGER RNA; and codon-nonspecific class-II release factors.

Conformational transitions of the shape of a protein to various unfolded states.

Integral membrane protein of Golgi and endoplasmic reticulum. Its homodimer is an essential component of the gamma-secretase complex that catalyzes the cleavage of membrane proteins such as NOTCH RECEPTORS and AMYLOID BETA-PEPTIDES precursors. PSEN1 mutations cause early-onset ALZHEIMER DISEASE type 3 that may occur as early as 30 years of age in humans.

A rigorously mathematical analysis of energy relationships (heat, work, temperature, and equilibrium). It describes systems whose states are determined by thermal parameters, such as temperature, in addition to mechanical and electromagnetic parameters. (From Hawley's Condensed Chemical Dictionary, 12th ed)

Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.

Evolution of Nova-dependent splicing regulation in the brain. (1/71)

A large number of alternative exons are spliced with tissue-specific patterns, but little is known about how such patterns have evolved. Here, we study the conservation of the neuron-specific splicing factors Nova1 and Nova2 and of the alternatively spliced exons they regulate in mouse brain. Whereas Nova RNA binding domains are 94% identical across vertebrate species, Nova-dependent splicing silencer and enhancer elements (YCAY clusters) show much greater divergence, as less than 50% of mouse YCAY clusters are conserved at orthologous positions in the zebrafish genome. To study the relation between the evolution of tissue-specific splicing and YCAY clusters, we compared the brain-specific splicing of Nova-regulated exons in zebrafish, chicken, and mouse. The presence of YCAY clusters in lower vertebrates invariably predicted conservation of brain-specific splicing across species, whereas their absence in lower vertebrates correlated with a loss of alternative splicing. We hypothesize that evolution of Nova-regulated splicing in higher vertebrates proceeds mainly through changes in cis-acting elements, that tissue-specific splicing might in some cases evolve in a single step corresponding to evolution of a YCAY cluster, and that the conservation level of YCAY clusters relates to the functions encoded by the regulated RNAs. (+info)

Amyloid-beta oligomers impair fear conditioned memory in a calcineurin-dependent fashion in mice. (2/71)

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Mechanisms of AD neurodegeneration may be independent of Abeta and its derivatives. (3/71)

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Glutamate carboxypeptidase II: an amyloid peptide-degrading enzyme with physiological function in the brain. (4/71)

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The heparan sulfate motif (GlcNS6S-IdoA2S)3, common in heparin, has a strict topography and is involved in cell behavior and disease. (5/71)

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Report of the task force on designing clinical trials in early (predementia) AD. (6/71)

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Sulfated glycosaminoglycans accelerate transthyretin amyloidogenesis by quaternary structural conversion. (7/71)

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Novel CHST6 gene mutations in 2 unrelated cases of macular corneal dystrophy. (8/71)

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Varkey J, Langen R (July 2017). "Membrane remodeling by amyloidogenic and non-amyloidogenic proteins studied by EPR". Journal ... Giasson BI, Lee VM, Trojanowski JQ (2003). "Interactions of amyloidogenic proteins". Neuromolecular Medicine. 4 (1-2): 49-58. ... Alpha-synuclein is a protein that, in humans, is encoded by the SNCA gene. Alpha-synuclein is a neuronal protein that regulates ... The majority form of the protein, and the one most investigated, is the full-length protein of 140 amino acids. Other isoforms ...

https://en.wikipedia.org/wiki/Alpha-synuclein

Kelly JW (February 1996). "Alternative conformations of amyloidogenic proteins govern their behavior". Curr. Opin. Struct. Biol ... These proteins include: transthyretin (ATTR, the most commonly implicated protein), apolipoprotein A1, and gelsolin. Due to the ... Ghoshdastider U, Popp D, Burtnick LD, Robinson RC (2013). "The expanding superfamily of gelsolin homology domain proteins". ... The aggregation of one precursor protein leads to peripheral neuropathy and/or autonomic nervous system dysfunction. ...

https://en.wikipedia.org/wiki/Familial_amyloid_neuropathy

FAP is characterized by the systemic deposition of amyloidogenic variants of the transthyretin protein, especially in the ... "Novel methods for detecting amyloidogenic proteins in transthyretin related amyloidosis". Frontiers in Bioscience. 13 (13): ... The transthyretin protein is a tetramer. The tetramer has to dissociate into misfolded monomers to aggregate into a variety of ...

https://en.wikipedia.org/wiki/Familial_amyloid_polyneuropathy

FAP is characterized by the systemic deposition of amyloidogenic variants of the transthyretin protein, especially in the ... Ando Y, Ueda M (May 2008). "Novel methods for detecting amyloidogenic proteins in transthyretin related amyloidosis". Frontiers ... The transthyretin protein is a tetramer. The tetramer has to dissociate into misfolded monomers to aggregate into a variety of ...

https://en.wikipedia.org/wiki/Transthyretin-related_hereditary_amyloidosis

April 2001). "Human cystatin C, an amyloidogenic protein, dimerizes through three-dimensional domain swapping". Nature ... an amyloidogenic protein, dimerizes through three-dimensional domain swapping" (PDF). Nature Structural Biology. 8 (4): 316-20 ... it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Cystatin C belongs to ... Cystatin C was first described as 'gamma-trace' in 1961 as a trace protein together with other ones (such as beta-trace) in the ...

https://en.wikipedia.org/wiki/Cystatin_C

... it has been speculated to play a role in amyloid disease and it was found to be a stable form for amyloidogenic proteins in ... Notably, the protein lysozyme is among the few native-state proteins shown to contain an alpha-strand region; lysozyme from ... 2008). The geometry of alpha-sheet: Implications for its possible function as amyloid precursor in proteins Proteins 71:425-431 ... prion proteins, and transthyretin repeats, all of which are associated with protein misfolding disease. For example, amyloid ...

https://en.wikipedia.org/wiki/Alpha_sheet

Liu T, Bitan G (March 2012). "Modulating self-assembly of amyloidogenic proteins as a therapeutic approach for ... Oxidative stress can directly cause neuron cell death or it can trigger a cascade of events that leads to protein misfolding, ... heat shock protein 70 (HSP-70)), and concomitantly downregulates pro-apoptotic factors. Lithium has been shown to reduce ... "G-protein-coupled receptor 30 mediates rapid neuroprotective effects of estrogen via depression of NR2B-containing NMDA ...

https://en.wikipedia.org/wiki/Neuroprotection

2001). "Human cystatin C, an amyloidogenic protein, dimerizes through three-dimensional domain swapping" (PDF). Nat. Struct. ... Cystatin-SA is a protein that in humans is encoded by the CST2 gene. The cystatin superfamily encompasses proteins that contain ... 1984). "Protein inhibitors of cysteine proteinases. III. Amino-acid sequence of cystatin from chicken egg white". Hoppe- ... The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, ...

https://en.wikipedia.org/wiki/CST2

Rhoades studies intrinsically disordered proteins and amyloidogenic proteins involved in Parkinson's disease, Alzheimer's ... focusing on understanding structural plasticity in intrinsically disordered proteins. These proteins do not form stable ... Rhoades E, Cohen M, Schuler B, Haran G (November 2004). "Two-state folding observed in individual protein molecules". J. Am. ... Research in the Rhoades lab aims to elucidate the principles that link protein conformational change with structure-function ...

https://en.wikipedia.org/wiki/Elizabeth_Rhoades

... will cleave amyloid-beta precursor protein (APP) into various types of amyloid beta (Aβ) protein. Most β-secretase activity ... CSF BACE1 activity could be a potential candidate biomarker to monitor amyloidogenic APP metabolism in the CNS. APP is an ... When Aβ peptide is released by proteolytic cleavage of amyloid-beta precursor protein, some Aβ peptides that are solubilized ... Amyloid plaque Aβ protein species ends in residue 40 or 42, but it is suspected that Aβ42 form is crucial in the pathogenesis ...

https://en.wikipedia.org/wiki/Alzheimer's_disease_biomarkers

It is associated with the systemic production and release of many amyloidogenic proteins, especially immunoglobulin light chain ... Amyloidosis and Other Protein Deposition Diseases", Emery and Rimoin's Principles and Practice of Medical Genetics, Oxford: ... Infiltrative and Protein Misfolding Myocardial Diseases", Muscle, Boston/Waltham: Academic Press, pp. 625-637, doi:10.1016/b978 ... diastolic dysfunction congestive heart failure arrythmia cardiac nervous conduction block fatigue dyspnea Amyloid proteins are ...

https://en.wikipedia.org/wiki/Amyloid_cardiomyopathy

... a metalloprotease from Alzheimer's disease brain able to degrade the beta-amyloid precursor protein and generate amyloidogenic ... 2001). "The neuropeptide processing enzyme EC 3.4.24.15 is modulated by protein kinase A phosphorylation". J. Biol. Chem. 275 ( ... Lehner B, Sanderson CM (2004). "A Protein Interaction Framework for Human mRNA Degradation". Genome Res. 14 (7): 1315-23. doi: ...

https://en.wikipedia.org/wiki/THOP1

... localization in human cerebral cortex and generation of amyloidogenic fragments from the beta-amyloid precursor protein". ... Cathepsin G is a protein that in humans is encoded by the CTSG gene. It is one of the three serine proteases of the ... Gabay JE, Scott RW, Campanelli D, Griffith J, Wilde C, Marra MN, Seeger M, Nathan CF (July 1989). "Antibiotic proteins of human ... Cathepsin G is a 255-amino-acid-residue protein including an 18-residue signal peptide, a two-residue activation peptide at the ...

https://en.wikipedia.org/wiki/Cathepsin_G

... have also been implicated in similar maturation events for other transmembrane proteins such as MHC class I proteins. Recent ... Thus, alpha-secretase cleavage precludes amyloid beta formation and is considered to be part of the non-amyloidogenic pathway ... "Protein kinase C-dependent alpha-secretase competes with beta-secretase for cleavage of amyloid-beta precursor protein in the ... Other ADAM proteins, ADAM17 (also called TACE, tumor necrosis factor-α converting enzyme), ADAM9, and ADAM19 have also been ...

https://en.wikipedia.org/wiki/Alpha_secretase

... amyloidogenic properties of PrP and known fungal proteins. As in yeast, proteins involved in gene expression and RNA binding ... One idea, the "Protein X" hypothesis, is that an as-yet unidentified cellular protein (Protein X) enables the conversion of ... While asserting that the flow of sequence information from protein to protein, or from protein to RNA and DNA was "precluded", ... Prions are misfolded proteins with the ability to transmit their misfolded shape onto normal variants of the same protein. They ...

https://en.wikipedia.org/wiki/Prion

The crystallographic structures show that short stretches from amyloid-prone regions of amyloidogenic proteins run ... Functional amyloid in Homo sapiens: Intralumenal domain of melanocyte protein PMEL Peptide/protein hormones stored as amyloids ... can induce the polymerization of essential amyloidogenic proteins, which should be deleterious to cells. Also, interaction ... protein receptors, soluble proteins, RNAs, small metabolites, etc. In the clinical setting, amyloid diseases are typically ...

https://en.wikipedia.org/wiki/Amyloid

Disrupting self-assembly and toxicity of amyloidogenic protein oligomers by "molecular tweezers" - from the test tube to animal ... Examples include the proteins involved in Alzheimer's disease - amyloid β-protein (Aβ) and tau; α-synuclein, which is thought ... efficiently inhibit the formation of toxic oligomers and aggregates by amyloidogenic proteins associated with different ... aggregation-prone mutants of the tumor-suppressor protein p53; and semen proteins whose aggregation enhances HIV infection. ...

https://en.wikipedia.org/wiki/Molecular_tweezers

Also, the IPOD is the sub-cellular site to which amyloidogenic proteins are sequestered in yeast, and where prions like [PSI+] ... When a reporter protein is fused with a prion domain, it forms a chimeric protein that demonstrates the conformational ... This transmission of protein state represents an epigenetic phenomenon where information is encoded in the protein structure ... cells is due to a reduced amount of functional Sup35 because much of the protein is in the amyloid state. The Sup35 protein ...

https://en.wikipedia.org/wiki/Fungal_prion

One major research focus is the role of protein misfolding in the onset of amyloidogenic diseases, including dialysis-related ... Proteins in this outer membrane facilitate transport with the outside world. These proteins share a similar nanoscale structure ... "Pore-like proteins designed from scratch". EurekAlert!. Retrieved 14 March 2021. "Pore-like proteins designed from scratch". ... "Protein-protein interactions in the early stages of amyloid assembly mechanisms". Wellcome. Retrieved 14 March 2021. "' ...

https://en.wikipedia.org/wiki/Sheena_Radford

It also inhibits production of amyloidogenic A beta peptide and increases secretion of soluble amyloid precursor protein via ...

https://en.wikipedia.org/wiki/CI-1017

AD is also considered a tauopathy due to abnormal aggregation of the tau protein, a microtubule-associated protein expressed in ... Alpha Secretase can render a non-pathological (non-amyloidogenic) Amyloid Beta (DOI: 10.2174/156720512799361655). Recently, a ... AD is also considered a tauopathy due to abnormal aggregation of the tau protein, a microtubule-associated protein expressed in ... protein in the brain. Amyloid beta is a short peptide that is an abnormal proteolytic byproduct of the transmembrane protein ...

https://en.wikipedia.org/wiki/Biochemistry_of_Alzheimer's_disease

... was used to accurately predict which regions of amyloidogenic proteins were most likely to make amyloid-like fibrils. By taking ... [email protected] aims to predict protein-protein docking and design new proteins with the help of about fifty-five thousand active ... July 2003). "Protein-protein docking predictions for the CAPRI experiment". Proteins. 52 (1): 118-22. CiteSeerX 10.1.1.80.9354 ... Wang C, Schueler-Furman O, Baker D (May 2005). "Improved side-chain modeling for protein-protein docking". Protein Science. 14 ...

https://en.wikipedia.org/wiki/[email protected]

Although studies in vitro and in yeast suggest that the acyl-CoA binding protein (ACBP) may modulate long-chain fatty acyl-CoA ... Huttunen HJ, Greco C, Kovacs DM (April 2007). "Knockdown of ACAT-1 Reduces Amyloidogenic Processing of APP". FEBS Letters. 581 ... A follow-up study reports that SOAT1 RNAi reduced cellular SOAT1 protein and cholesteryl ester levels while causing a slight ... Huang H, Atshaves BP, Frolov A, Kier AB, Schroeder F (August 2005). "Acyl-coenzyme A binding protein expression alters liver ...

https://en.wikipedia.org/wiki/Sterol_O-acyltransferase

Amyloidogenic proteins, such as the Huntingtin protein, are the IPOD's substrates. Misfolded proteins must be non-ubiquitinated ... microscope Fluorescent protein Heat shock proteins Molecular chaperones Neurodegeneration Prions Protein aggregation Protein ... The IPOD was shown to be the sub-cellular site to which toxic amyloidogenic proteins are sequestered to, hereby serving as a ... For many years, protein aggregation was considered a random process by which misfolded proteins stick to each other to form ...

https://en.wikipedia.org/wiki/JUNQ_and_IPOD

In tissue culture, the enzyme has been found to generate amyloidogenic fragments from the amyloid precursor protein, suggesting ... Kallikrein-6 is a protein that in humans is encoded by the KLK6 gene. Kallikreins are a subgroup of serine proteases having ... a novel and potentially amyloidogenic enzyme cDNA isolated from Alzheimer's disease brain". The Journal of Biological Chemistry ... "Immunofluorometric quantitation and histochemical localisation of kallikrein 6 protein in ovarian cancer tissue: a new ...

https://en.wikipedia.org/wiki/KLK6

Deposition patterns vary between people but are almost always composed of just one amyloidogenic protein. Deposition can be ... The type of the amyloid protein can be determined in various ways: the detection of abnormal proteins in the bloodstream (on ... The vast majority of proteins that have been found to form amyloid deposits are secreted proteins, so the misfolding and ... the protein aggregation may be associated with aging-related decline in protein regulation. Rarely, some medical treatments are ...

https://en.wikipedia.org/wiki/Amyloidosis

... and protein trafficking. The proteins' role in calcium homeostasis in neurons has been a subject of interest. The genetic ... clear from subsequent work that the mutations were associated with higher proportions of Aβ42 over the less amyloidogenic Aβ40 ... The two proteins differ in subcellular localization, with PS1 expressed more broadly and present at the cell membrane, while ... Gamma secretase can cut APP at several points within a small region of the protein, which results in Aβ of various lengths. The ...

https://en.wikipedia.org/wiki/Presenilin

In 1999, he became director of the University College London Centre for Amyloidosis and Acute Phase Proteins. Mark Pepys has ... as well as novel amyloidogenic variants of apolipoprotein AI and transthyretin. ... He has pioneered work on the structure, function and clinical applications of the pentraxins, C-reactive protein (CRP) and ... the pentraxin proteins, and amyloidosis, and is a leading authority on these subjects in the UK and internationally. He ...

https://en.wikipedia.org/wiki/Mark_Pepys

Eisenberg's current research focuses on the structural biology of amyloidogenic proteins, while his computational efforts ... This recently recognized protein state provides opportunities to understand cells in health and disease. 1961 - L.J. Henderson ... Lüthy, R.; Bowie, J. U.; Eisenberg, D. (1992). "Assessment of protein models with three-dimensional profiles". Nature. 356 ( ... Eisenberg, D.; McLachlan, A. D. (1986). "Solvation energy in protein folding and binding". Nature. 319 (6050): 199-203. doi: ...

https://en.wikipedia.org/wiki/David_Eisenberg

Contact Order Folding Rate 3D Folding Decoys Amyloidogenic Proteins David S. Wishart, David Arndt, Mark Berjanskii, An Chi Guo ... The Protein Property Prediction and Testing Database (PPT-DB) is a collection of protein property databases for over 20 ... Yi Shi, Savita Shrivastava, Jianjun Zhou, You Zhou and Guohui Lin: PPT-DB: the protein property prediction and testing database ... different protein properties including secondary structure, trans-membrane helices and beta barrels, accessible surface area, ...

https://en.wikipedia.org/wiki/PPT-DB

protein processing. • protein maturation. • myeloid dendritic cell differentiation. • autophagy. • protein glycosylation. • ... of presenilin-1 can down regulate amyloid and inhibition of presenilin-1 can be a potential method for anti-amyloidogenic ... positive regulation of protein kinase activity. • T cell activation involved in immune response. • cellular protein metabolic ... positive regulation of protein binding. • positive regulation of protein import into nucleus, translocation. • Notch receptor ...

https://en.wikipedia.org/wiki/PSEN1

Proisletni amiloidni polipeptid (proIAPP, proamilin, proislet protein) proizvodi se u beta ćelijama (β-ćelijama) gušterače kao ... "Membrane Fragmentation by an Amyloidogenic Fragment of Human Islet Amyloid Polypeptide Detected by Solid-State NMR ... "Amyloid fibrils in human insulinoma and islets of Langerhans of the diabetic cat are derived from a neuropeptide-like protein ...

https://bs.wikipedia.org/wiki/Amilin

Protein precursors include immunoglobulin-derived light chains and transthyretin mutations.[2] The misfolding of the protein ... such as chemotherapy for amyloidogenic plasma cell dyscrasia.[2] Drugs can be prescribed including midodrine for autonomic ... Cardiac amyloidosis is a subcategory of amyloidosis where there is the depositing of the protein amyloid in the cardiac muscle ... Amyloid, a misfolded and insoluble protein, can become a deposit in the heart's atria, valves, or ventricles. These deposits ...

https://en.wikipedia.org/wiki/Cardiac_amyloidosis

protein destabilization. • protein homooligomerization. • amyloid fibril formation. • G-protein coupled receptor signaling ... "Membrane Fragmentation by an Amyloidogenic Fragment of Human Islet Amyloid Polypeptide Detected by Solid-State NMR ... identical protein binding. • amyloid-beta binding. • protein binding. Cellular component. • extracellular region. • neuronal ... cellular protein metabolic process. • negative regulation of cell differentiation. • cell-cell signaling. • nociception. • ...

https://en.wikipedia.org/wiki/Amylin

Tertiary Protein Structure and Folds: section 4.3.2.1. From Principles of Protein Structure, Comparative Protein Modelling, and ... There is also evidence that parallel β-sheet may be more stable since small amyloidogenic sequences appear to generally ... Richardson JS (1981). Anatomy and Taxonomy of Protein Structures. Advances in Protein Chemistry. 34. pp. 167-339. doi:10.1016/ ... Hutchinson EG, Thornton JM (1990). "HERA--a program to draw schematic diagrams of protein secondary structures". Proteins. 8 (3 ...

https://en.wikipedia.org/wiki/Beta_sheet

... are involved in protein-protein interactions. The SNX8 protein, even though is very similar to the other sorting nexins, ... an amyloidogenic pathway via acidified late endosomes and a non-amyloidogenic or anti-myloidogenic retrograde pathway via Golgi ... SNX8 protein regulates cholesterol levels as an activator of the SREBPs (Sterol Regulatory Element Binding Proteins), which is ... As a protein of the SNXs family, the SNX8 is formed of 465 aminoacids and presents a BAR-domain and a PX-domain which are very ...

https://en.wikipedia.org/wiki/SNX8

The N-terminal helices 1 and 3 have been identified as amyloidogenic peptides of SAA1.1, that are not presence on protein ... Serum amyloid A1 (SAA1) is a protein that in humans is encoded by the SAA1 gene. SAA1 is a major acute-phase protein mainly ... Hari-Dass R, Shah C, Meyer DJ, Raynes JG (May 2005). "Serum amyloid A protein binds to outer membrane protein A of gram- ... In addition to its association with HDL, SAA1 interacts with a number of mammalian proteins, mostly cell surface proteins such ...

https://en.wikipedia.org/wiki/Serum_amyloid_A1

This achievement is a far-reaching extension of the concept of protein aggregation to include a significantly larger class of ... Energy Landscape of Amyloidogenic Peptide Oligomerization by Parallel-tempering Molecular Dynamics Simulation: Significant Role ... This discovery was a directed continuation of his achievements in the study of protein amyloids, including the pioneering ...

https://en.wikipedia.org/wiki/Ehud_Gazit

... such as protein-protein interactions, protein-DNA interactions, and protein conformational changes. For monitoring the complex ... March 2006). "Quantitative determination of the topological propensities of amyloidogenic peptides". Biophysical Chemistry. 120 ... FRET can be used to observe membrane fluidity, movement and dispersal of membrane proteins, membrane lipid-protein and protein- ... Truong K, Ikura M (October 2001). "The use of FRET imaging microscopy to detect protein-protein interactions and protein ...

https://en.wikipedia.org/wiki/Förster_resonance_energy_transfer

... biochemistry and structural biology as a pioneer in the study of the prion-like and amyloidogenic behavior of the p53 protein ... While studying with Weber, he deepened his knowledge of proteins, studying protein plasticity, supramolecular structures, and ... publishing important studies on protein folding and the relationship between protein aggregates and diseases such as cancer, ... His work has led to advances in the understanding of protein folding, virus assembly, and the mechanisms responsible for the ...

https://en.wikipedia.org/wiki/Jerson_Lima_Silva

... and that amyloidogenic proteins in their fibrillar form may be relatively benign. In most, if not all proteopathies, a change ... proteins that assist protein folding) may antagonize proteotoxicity during aging and in protein misfolding-diseases to maintain ... Some proteins can be induced to form abnormal assemblies by exposure to the same (or similar) protein that has folded into a ... Often the proteins fail to fold into their normal configuration; in this misfolded state, the proteins can become toxic in some ...

https://en.wikipedia.org/wiki/Proteopathy

... and in from protein. The main component of gintonin is a complex of lysophosphatidic acids (LPA) and ginseng proteins such as ... Gintonin also shows in vivo anti-Alzheimer's disease effects through LPA receptor-mediated non-amyloidogenic pathways and ... is novel lysophosphatidic acids-protein complexes and activates G protein-coupled lysophosphatidic acid receptors with high ... is novel lysophosphatidic acids-protein complexes and activates G protein-coupled lysophosphatidic acid receptors with high ...

https://en.wikipedia.org/wiki/Gintonin

An Introduction to the ProteOn™ XPR36 Protein Interaction Array System - Duration: 1:03:25. Bio-Rad Laboratories 1,777 views ... Protein Purification & Characterization - Duration: 27:09. David A. Johnson, PhD 49,950 views ... These approaches are very useful in screening mutations or potential anti-amyloidogenic drugs for their effects on ... and tissue deposition of specific proteins. A well-known example is Alzheimers disease (AD). The condition is characterized by ...

https://www.youtube.com/watch?v=ziw-bMeUzNc

Those associated with amyloidogenic proteins have often been lumped together simply because those proteins aggregate. However, ... Amyloidogenic metal-binding proteins: new investigative pathways. Biochemical Society Transactions, 36, pp. 1299-1303. ... The associated proteins not only aggregate, but also bind copper. The APP (amyloid precursor protein) binds copper in an N- ... Binding of copper has been suggested to influence generation of beta-amyloid from the protein. PrP (prion protein) binds copper ...

http://opus.bath.ac.uk/12392/

Until now, such approaches to define the direct effects of amyloidogenic PrP protein on cells have only been fragmentary. The ... Basic Mechanism of Neurodegeneration in Transmissible Spongiform Encephalopathies : Role of Amyloidogenic prion protein PrP in ... The pathology is characterized by accumulations of protease- resistant isoforms of the prion protein PrP (PrPSc), ... to investigate whether the structural state of the protein is critical in its pathological effects. ...

https://cordis.europa.eu/project/id/BMH4960601

Aberrant protein folding and self-assembly underlie over 30 human diseases called amyloidoses, for which currently there is no ... Amyloidogenic proteins Degenerative/neurodegenerative diseases Mechanisms of disease Non-fibrillar oligomeric assemblies ... Preparation and Structural Characterization of Pre-fibrillar Assemblies of Amyloidogenic Proteins Anat Frydman-Marom, Yaron ... Coverage of the pathologic and pathogenic structures of amyloidogenic proteins from the pathological lesions to the evasive ...

https://rd.springer.com/book/10.1007%2F978-94-007-2774-8

... that an amyloidogenic protein, CsgA, present in E. coli biofilms in the gut can trigger Parkinsons disease in mice. This study ...

https://content.iospress.com/articles/journal-of-parkinsons-disease/jpd202085

Alzheimer Aβ peptides or peptides being fragments of amyloidogenic proteins), to large proteins (transthyretin, prion protein, ... The number of papers published on simulations of amyloidogenic proteins has increased rapidly during the last decade. The ... investigations could improve our understanding of the protein aggregation and fibrillization process of amyloidogenic proteins ... Experimental studies of the nature of protein aggregation are unfortunately limited by the structure of aggregates and their ...

https://repozytorium.bg.ug.edu.pl/info/article/UOG005a20ac9e9d41daa54519e39206bdfb/

... amyloidogenic proteins, such as beta amyloid (Aβ)-42 and prion protein (Prp)106-126, the neuroprotective peptide, humanin, ... protein kinase B (Akt) pathway, the mitogen-activated protein kinase (MAPK) pathway, p38MAPK, as well as the phosphorylation of ... FPR2 is activated by an array of ligands, which include structurally unrelated lipids and peptide/proteins agonists, resulting ... Upon activation, intracellular domains of FPR2 mediate signaling to G-proteins, which trigger several agonist-dependent signal ...

https://www.mdpi.com/1422-0067/14/4/7193/htm

Purification of recombinant amyloidogenic proteins can sometimes prove challenging due in part to their aggregation-prone ... Profinity™ IMAC Resin: An Optimal Support for His-Tagged Protein Purification Engineering Antibodies: A Fab-ulous Future for ... Application of UNOsphere SUPrA™ Media, a New Protein A Affinity Chromatographic... Purification of a Monoclonal Antibody Using ... Over 100 amyloidogenic mutations have been reported and they represent the most common cause of hereditary amyloidosis. ...

http://futurelab.biocompare.com/4-production/article/appnote-purification-amyloidogenic-transthyretin-protein

Amyloidogenic processing of the chimeric proteins APP-LAMP and APP-MPR. We first examined the formation of the C-terminal ... 1996) Amyloidogenic processing of the human amyloid precursor protein in primary cultures of rat hippocampal neurons. J ... The proteins are N-terminally tagged to a 10 aa c-myc sequence. b, Detection of the chimeric APP proteins by immunoblotting. ... 1992) Processing of the amyloid protein precursor to potentially amyloidogenic derivatives. Science 255:728-730. ...

http://www.jneurosci.org/content/17/20/7714

... amyloid precursor protein (APP), and BACE1 and robustly ... RanBP9 binds low-density lipoprotein receptor-related protein ( ... Role of RanBP9 on amyloidogenic processing of APP and synaptic protein levels in the mouse brain. ... We previously reported that RanBP9 binds low-density lipoprotein receptor-related protein (LRP), amyloid precursor protein (APP ... Conversely, RanBP9-null mice showed increased levels of synaptophysin, PSD-95, and drebrin A protein levels. Given that loss of ...

https://research.pasteur.fr/en/publication/role-of-ranbp9-on-amyloidogenic-processing-of-app-and-synaptic-protein-levels-in-the-mouse-brain/

1996) Amyloidogenic processing of the human amyloid precursor protein in primary cultures of rat hippocampal neurons. J ... 1996) The profile of soluble amyloid beta protein in cultured cell media. Detection and quantification of amyloid beta protein ... insoluble amyloidogenic fragments of the amyloid precursor protein in transfected cells. J Biol Chem 270:14786-14792. ... Aβ is derived by proteolysis of larger transmembrane proteins, termed amyloid precursor proteins (APP). APP isoforms are ...

http://www.jneurosci.org/content/18/23/9629

Altered dimer interface decreases stability in an amyloidogenic protein. Elizabeth M. Baden, Barbara A.L. Owen, Francis C. ... Altered dimer interface decreases stability in an amyloidogenic protein. Journal of Biological Chemistry. 2008 Jun 6;283(23): ... Altered dimer interface decreases stability in an amyloidogenic protein. / Baden, Elizabeth M.; Owen, Barbara A.L.; Peterson, ... Fingerprint Dive into the research topics of Altered dimer interface decreases stability in an amyloidogenic protein. ...

https://mayoclinic.pure.elsevier.com/en/publications/altered-dimer-interface-decreases-stability-in-an-amyloidogenic-p

... cavity which will bind thyroxine and an exterior binding site for the complex of retinol with retinol-binding protein. The ... The x-ray crystal structure refinements of normal human transthyretin and the amyloidogenic Val-30Met variant to 1.7-A ... The x-ray crystal structures of normal human transthyretin (prealbumin) and the amyloidogenic Val-30-Met variant have been ... The x-ray crystal structures of normal human transthyretin (prealbumin) and the amyloidogenic Val-30-Met variant have been ...

https://www.rcsb.org/structure/1TTA

In lysozyme and the prion protein, α-sheets formed in the specific regions of the protein implicated in the amyloidogenic ... In contrast to the amyloidogenic proteins presented here, these normal proteins do not form extensive extended α-sheet ... Amyloidogenic mutations in the N-terminal region of the protein may perturb the equilibrium between segments of PII and α- ... MD-generated α-sheet intermediate structures for four amyloidogenic proteins: transthyretin (a), β2m (b), D67H lysozyme (c), ...

https://www.pnas.org/content/101/32/11622.long

The x-ray crystal structure refinements of normal human transthyretin and the amyloidogenic Val-30Met variant to 1.7-A ... TRANSTHYRETIN protein, length: 127 (BLAST) Sequence Similarity Cutoff. Rank. Chains in Cluster. Cluster ID / Name. Structural ... THE X-RAY CRYSTAL STRUCTURE REFINEMENTS OF NORMAL HUMAN TRANSTHYRETIN AND THE AMYLOIDOGENIC VAL 30MET VARIANT TO 1.7 ... Structures of protein chains with identical sequences (sequence identity > 95%) are aligned, superimposed and clustered. ...

http://www.rcsb.org/pdb/explore/sequenceCluster.do?structureId=1ETA&entity=1&seqid=95

Capsaicin promotes the amyloidogenic route of brain amyloid precursor protein processing. Magdolna Pákáski, Marietta Hugyecz, ... Capsaicin promotes the amyloidogenic route of brain amyloid precursor protein processing. / Pákáski, Magdolna; Hugyecz, ... Capsaicin promotes the amyloidogenic route of brain amyloid precursor protein processing. In: Neurochemistry international. ... Capsaicin promotes the amyloidogenic route of brain amyloid precursor protein processing. Neurochemistry international. 2009 ...

https://hungary.pure.elsevier.com/en/publications/capsaicin-promotes-the-amyloidogenic-route-of-brain-amyloid-precu

Evolvability of Amyloidogenic Proteins in Human Brain.. J Alzheimers Dis. 2018;62(1):73-83. PubMed: 29439348 ... Atomic structures of low-complexity protein segments reveal kinked β sheets that assemble networks.. Science. 2018 Feb 9;359( ... RESEARCH NEWS 2018-02-16 Research News Many proteins involved in neurodegeneration have a penchant to undergo liquid phase ... Quantitative Measurement of γ-Secretase-mediated Amyloid Precursor Protein and Notch Cleavage in Cell-based Luciferase Reporter ...

https://www.alzforum.org/search?commentator=Dello%20Russo%2CCinzia

Evolvability of Amyloidogenic Proteins in Human Brain.. J Alzheimers Dis. 2018;62(1):73-83. PubMed: 29439348 ... Atomic structures of low-complexity protein segments reveal kinked β sheets that assemble networks.. Science. 2018 Feb 9;359( ... Quantitative Measurement of γ-Secretase-mediated Amyloid Precursor Protein and Notch Cleavage in Cell-based Luciferase Reporter ...

https://www.alzforum.org/search?commentator=Hard%2CTorleif&page=1

... protein assemblies and in-cell NMR. This exposition of an invaluable technique will interest those working in a range of ... NMR Studies of Small Molecules Interacting with Amyloidogenic Proteins. Elke Prade, Juan-Miguel Lopez del Amo and Bernd Reif ... Large Protein Complexes Revealed by Solution-State NMR: G Proteins and G Protein-Activated Inwardly Rectifying Potassium Ion ... Topics include applications to biological membranes, membrane active peptides, membrane proteins, protein assemblies and in- ...

https://pubs.rsc.org/en/content/ebook/978-1-84973-910-8

Moreover, amyloidogenic regions also correspond to the structured regions. We analyzed the amino acid composition of [Formula: ... Formula: see text]-crystallin is the major eye lens protein and a member of the small heat-shock protein (sHsp) family. [ ... Search for conserved amino acid residues of the α-crystallin proteins of vertebrates.. Shiliaev NG1, Selivanova OM1, ... 1 Institute of Protein Research, Russian Academy of Sciences, Institutskaya str., 4 Pushchino, Moscow Region 142290, Russia.. ...

https://www.ncbi.nlm.nih.gov/pubmed/26972563

Effects of amyloidogenic proteins on membrane disruption and [Ca2+]i elevations. (a) Effects of amyloidogenic proteins and ... and other amyloidogenic proteins using gel electrophoresis and AFM imaging, and demonstrated that these amyloidogenic proteins ... Other disease-related amyloidogenic proteins, such as prion protein in prion diseases or α-synuclein in dementia with Lewy ... Other amyloidogenic disease-related proteins, such as the prion protein or α-synuclein, also exhibit similarities in the ...

https://www.hindawi.com/journals/ijad/2011/304583/

A cleavage agent selectively acting on soluble assembly of an amyloidogenic peptide or a protein is represented by the formula( ... peptide or a protein by connecting sites selectively recognizing Soluble assembly of the amyloidogenic peptide or a protein ... A cleavage agent is provided to reduce the amount of soluble oligomers of an amyloidogenic ... peptide or protein or the precursor protein to stabilize the initial display of the amyloidogenic peptides or proteins a is to ...

https://patents.google.com/patent/KR20080036902A/en

... peptide and full-length tau protein in vitro, in a dose-dependent manner as evident from ThS assay, CD spectroscopy, and TEM. ... characterized by the conversion of natively unfolded monomeric protein/peptide into misfolded β-sheet rich aggregates. ... Intra-cellular tau protein tangles and extra-cellular β-amyloid plaques are hallmarks of Alzheimers disease (AD), ... Generic inhibition of amyloidogenic proteins by two naphthoquinone-tryptophan hybrid molecules. Proteins 80, 1962-1973 (2012). ...

https://www.nature.com/articles/s41598-017-18443-2?error=cookies_not_supported&code=83b52cab-59b6-4988-988b-e3edd1f8b3db

We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their ... InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites ... In the non-amyloidogenic (non-plaque-forming) pathway, APP is cleaved by alpha-secretase to yield a soluble N-terminal sAPP- ... Amyloid beta A4 protein (also known as APP or A4) consists of a large N-terminal extracellular region containing heparin- ...

http://www.ebi.ac.uk/interpro/entry/IPR028866

Proteotoxicity in cardiac amyloidosis: Amyloidogenic light chains affect the levels of intracellular proteins in human heart ... Proteotoxicity in cardiac amyloidosis: Amyloidogenic light chains affect the levels of intracellular proteins in human heart ... Proteotoxicity in cardiac amyloidosis: amyloidogenic light chains affect the levels of intracellular proteins in human heart ... The proteome alterations affect proteins involved in cytoskeletal organization, protein synthesis and quality control, ...

https://repositori.upf.edu/handle/10230/35186

... and various proteins in the beta-amyloid precursor protein (APP) processing pathway. The strongest and most consistent effects ... Reductions in Abeta may be related to shifted APP processing toward the non-amyloidogenic pathway, because alpha-secretase ... Synergistic effects of long-term antioxidant diet and behavioral enrichment on beta-amyloid load and non-amyloidogenic ...

https://pubmed.ncbi.nlm.nih.gov/20660265/

The MspA protein discovered here could accordingly represent a more widely found sheath protein than the MspA from M. ... The MspA protein discovered here could accordingly represent a more widely found sheath protein than the MspA from M. ... The sheaths of Methanosaeta thermophila PT are composed of the 60.9 kDa major sheath protein MspA. In this study we show that ... The genome of M. hungatei JF-1 encodes six homologs of the identified MspA protein. Several homologs also exist in the related ...

https://www.frontiersin.org/articles/10.3389/fmicb.2018.02729/full

AL-Base is designed to compile and analyse amyloidogenic Ig LC sequences and to compare their predicted protein sequence and ... of which 433 encode monoclonal proteins that were reported to form fibrillar deposits in AL patients. Each sequence is ... structure to non-amyloidogenic LC sequences. Currently, the database contains over 3000 de-identified LC nucleotide and amino ... Altered dimer interface decreases stability in an amyloidogenic protein.. *Elizabeth M Baden, Barbara A. L. Owen, Francis C ...

https://www.semanticscholar.org/paper/AL-Base%3A-a-visual-platform-analysis-tool-for-the-of-Bodi-Prokaeva/228679f00366cedcedf60c8fcd6b797093bfb322

An Achilles heel in an amyloidogenic protein and its repair: Insulin fibrillation and therapeutic design. Journal of ... Fingerprint Dive into the research topics of An Achilles heel in an amyloidogenic protein and its repair: Insulin ... An Achilles heel in an amyloidogenic protein and its repair : Insulin fibrillation and therapeutic design. / Yang, Yanwu; ... title = "An Achilles heel in an amyloidogenic protein and its repair: Insulin fibrillation and therapeutic design", ...

https://augusta.pure.elsevier.com/en/publications/an-achilles-heel-in-an-amyloidogenic-protein-and-its-repair-insul

The crystal structure of amyloidogenic Leu55 --, Pro transthyretin variant reveals a possible pathway for transthyretin ... The crystal structure of amyloidogenic Leu55 Pro transthyretin variant reveals a possible pathway for transthyretin ...

http://www.ebi.ac.uk/pdbe/entry/pdb/5TTR

Similarly, other neurodegenerative diseases have recently been discovered to involve protein aggregation. (jci.org)
Preface [Hot Topic: Amyloidogenic Proteins and Peptides Involved in Human Neurodegenerative Diseases (Guest Editor: Brian m. (benthamscience.com)
Scientists have suspected that in the neurodegenerative diseases of mammals such as sheep scrapie, mad cow disease (or bovine spongiform encephalopathy) and the kuru disease of the Papua New Guinea tribes, a normal protein in the brain can somehow become twisted and then corrupt other, healthy molecules of the same protein to do likewise a process much like the seeding of a crystal. (mad-cow.org)
Although the yeast sup35 protein and the mammalian prion protein are not at all related to each other the yeast pose no risk to consumers of bread or beer the researchers think that in-depth analysis of the yeast prion-like elements and other proteins that help them fold up may lead to new approaches to therapies for neurodegenerative diseases. (mad-cow.org)
Stress and aging can challenge the proteostasis network, resulting in the aggregation of misfolded proteins-a feature of numerous neurodegenerative conditions. (cshlpress.com)
A number of proteins known to be associated with neurodegenerative diseases were also identified. (mcponline.org)
Detailed characterization of these proteins will supply the necessary information to appropriately interpret proteins associated with aging and/or age-related neurodegenerative diseases. (mcponline.org)
Thus, identification of proteins unique to each brain region, those associated with neurodegenerative mechanisms in particular, could yield opportunities to overcome major obstacles in the development of new protective and restorative therapies for prominent neurodegenerative diseases. (mcponline.org)
Specifically, GAPDH has been shown to interact with neurodegenerative disease-associated proteins, including the amyloid-β protein precursor (AβPP). (iospress.com)
Amyloidogenic neurodegenerative diseases are incurable conditions with high social impact that are typically caused by specific, largely disordered proteins. (plos.org)
The demonstrated ability to inhibit the conformational heterogeneity of these proteins by a single pharmacological agent reveals common features in the monomer and suggests a common pathway to diagnose, prevent, halt, or reverse multiple neurodegenerative diseases. (plos.org)

The APP (amyloid precursor protein) binds copper in an N-terminal region. (bath.ac.uk)
Generation of βA4 by proteolytic processing of the amyloid precursor protein (APP) is thought to occur in a pathway that includes the activity of two as yet unknown proteases, with β-secretase cleaving at the N terminus and γ-secretase releasing the C terminus of βA4. (jneurosci.org)
There are many different human amyloid diseases linked to a specific precursor protein or peptide ( 1 - 3 ), the most well known being Alzheimer's disease. (pnas.org)
An antibody was recently identified that is specific for these soluble oligomeric intermediates, but not the insoluble fibrils nor the soluble precursor protein ( 14 ). (pnas.org)
We previously reported that RanBP9 binds low-density lipoprotein receptor-related protein (LRP), amyloid precursor protein (APP), and BACE1 and robustly increased Aβ generation in a variety of cell lines and primary neuronal cultures. (pasteur.fr)
The principal component of amyloid deposits is β amyloid peptide (Aβ), a peptide derived by proteolytic processing of the amyloid precursor protein (APP). (jneurosci.org)
Aβ is derived by proteolysis of larger transmembrane proteins, termed amyloid precursor proteins (APP). (jneurosci.org)
In this study, the effects of capsaicin on the processing of amyloid precursor protein (APP) were investigated in an in vivo model. (elsevier.com)
Quantitative Measurement of γ-Secretase-mediated Amyloid Precursor Protein and Notch Cleavage in Cell-based Luciferase Reporter Assay Platforms. (alzforum.org)
Measures assessed were Abeta neuropathology in plaques, biochemically extractable Abeta(40) and Abeta(42) species, soluble oligomeric forms of Abeta, and various proteins in the beta-amyloid precursor protein (APP) processing pathway. (nih.gov)
The amyloid precursor protein and postnatal neurogenesis/neuroregeneration. (ebi.ac.uk)
Formation of senile plaques containing the beta-amyloid peptide (A beta) derived from the amyloid precursor protein (APP) is an invariant feature of Alzheimer's disease (AD). (uni-muenchen.de)
[ 36 ] Aβ accumulation results from increased synthesis and abnormal processing of amyloid precursor protein (APP) in s-IBM muscle. (medscape.com)
This is thought to be because of overproduction of the β-amyloid (Aβ) protein due to trisomy for the Aβ precursor protein gene on chromosome 21. (nih.gov)
Presymptomatic carriers of presenilin (PS-1) and Aβ precursor protein gene mutations who are destined to develop Alzheimer's disease also show preclinical amyloid deposition. (nih.gov)
An alpha-synuclein fragment, known as the non-Abeta component (NAC) of Alzheimer's disease amyloid, originally found in an amyloid-enriched fraction, was shown to be a fragment of its precursor protein, NACP. (wikipedia.org)
Increased amyloid beta (Aβ) production by sequential cleavage of the amyloid precursor protein (APP) by the β- and γ-secretases contributes to the etiological basis of Alzheimer's disease (AD). (semanticscholar.org)
Able to degrade the amyloid-beta precursor protein and generate amyloidogenic fragments. (uniprot.org)
Synthetic peptide corresponding to Human Amyloid Precursor Protein aa 653-662. (abcam.com)
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Amyloid Precursor Protein aa 700 to the C-terminus (phospho T743). (abcam.com)
Chloroquine inhibits intracellular degradation but not secretion of Alzheimer B/A4 amyloid precursor protein. (acnp.org)
Potentially amyloidogenic, carboxyl-terminal derivatives of the amyloid protein precursor. (acnp.org)
Evidence for a nonsecretory, acidic degradation pathway for amyloid precursor protein in 293 cells," J. Biol. (patentgenius.com)
Hydrolysis of amyloid precursor protein-derived peptides by cysteine proteinases and extracts of rat brain clathrin-coated vesicles," Peptides 15(1):175-182 (1994). (patentgenius.com)
amyloid precursor protein c-terminal fragments and the generation of amyloid .beta. (patentgenius.com)
Secretase Cleavage of Amyloid Precursor Protein," J. Of Biological Chem. (patentgenius.com)
Secretase Proteases Using Peptide and Amyloid Precursor Protein Substrates," J. Of Neurochem. (patentgenius.com)

An increasing number of diseases are now being associated with misfolding, aggregation, and tissue deposition of specific proteins. (youtube.com)
Aberrant protein folding and self-assembly underlie over 30 human diseases called amyloidoses, for which currently there is no cure. (springer.com)
The first decade of the 21st century has brought with it significant progress in our understanding of amyloid diseases, including the physiological and pathological processes involving each of the offending proteins. (springer.com)
This book covers the current state-of-the-art knowledge on amyloidoses as a general phenomenon and offers detailed reviews of individual amyloid-forming proteins and specific diseases. (springer.com)
Mechanism-based strategies for inhibiting protein aggregation and potential therapeutic applications in different diseases. (springer.com)
Other disease-related amyloidogenic proteins, such as prion protein in prion diseases or α -synuclein in dementia with Lewy bodies, exhibit similarities in the incorporation into membranes and the formation of calcium-permeable channels. (hindawi.com)
Insulin fibrillation provides a model for a broad class of amyloidogenic diseases. (elsevier.com)
As both diseases involve lesions containing the microtubule-associated protein tau, they are classed as tauopathies (see Box 3 for definitions). (mja.com.au)
For many decades, clinicians have been aware of the formation of insoluble protein aggregates in particular diseases. (jci.org)
Type 2 diabetes mellitus (T2DM) is classified as a protein-misfolding disease and shares the debilitating consequences of misfolded and aggregated peptides and proteins with more than 20 other diseases, such as Alzheimer's disease, Parkinson's disease, and spongiform encephalopathy [ 1 - 3 ]. (hindawi.com)
Insulin is a widely used peptide in protein research and it is utilized as a model peptide to understand the mechanics of fibril formation, which is believed to be the cause of diseases such as Alzheimer and Creutzfeld-Jakob syndrome. (frontiersin.org)
Amyloidoses represent a heterogeneous group of diseases characterized by abnormal protein metabolism leading to extracellular deposition of fibrillar, proteinaceous amyloid in various tissues and organs of the body. (diva-portal.org)
To date more than 20 different proteins have been linked to diseases with amyloid depositions, of which Alzheimer's disease and the prion-associated diseases are the most well known. (diva-portal.org)
FoldRx's portfolio includes clinical and pre-clinical programs for investigational compounds to treat diseases caused by protein misfolding, which is increasingly recognized as an underlying cause in many chronic degenerative diseases. (bio-medicine.org)
By combining FoldRx's proprietary expertise in identifying and developing treatments for protein misfolding diseases with Pfizer's commercial, medical and regulatory expertise, and global strengths in patient services and reimbursement, we are taking a significant step toward potentially bringing, for the first time, a non-surgical treatment option for underserved patients affected by the deadly disease ATTR-PN," said Geno Germano, president and general manager, Pfizer Specialty Care Business Unit. (bio-medicine.org)
Its screening engine is rapid and efficient in evaluating potential treatment candidates in a wide range of diseases caused by misfolded proteins. (bio-medicine.org)
Researchers at the University of Chicago's Howard Hughes Medical Institute have found that a protein molecule able to transmit a genetic trait without DNA or RNA in yeast is able to string itself together into long fibers much like those found in the brain in "mad cow" and human Creutzfeldt-Jakob diseases. (mad-cow.org)
Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Biology provides a comprehensive update on how the proteostasis network functions in healthy cells and the diseases that result when protein quality control goes awry. (cshlpress.com)
Protein-misfolding diseases (PMDs) are usually attributed to proteins that convert from the native state to β-sheet-rich aggregates under certain circumstances ( 3 ). (asm.org)
Biotechnologically engineered protein binders for applications in amyloid diseases. (uni-ulm.de)
There are about 28 amyloidogenic proteins, each of which is associated with diseases. (ucdavis.edu)
Are amyloid diseases caused by protein aggregates that mimic bacterial pore-forming toxins? (google.co.uk)

The authors present integrated computational approaches that require various degrees of computational complexity, and the major accomplishments that have been achieved in the design and structural characterization of helical peptides and proteins. (nhbs.com)

Molecular dynamics simulations, coupled with experimental investigations could improve our understanding of the protein aggregation and fibrillization process of amyloidogenic proteins. (edu.pl)

Oligomerization, conformational changes, and the consequent neurodegeneration of Alzheimer's β -amyloid protein (A β P) play crucial roles in the pathogenesis of Alzheimer's disease (AD). (hindawi.com)
Intra-cellular tau protein tangles and extra-cellular β-amyloid plaques are hallmarks of Alzheimer's disease (AD), characterized by the conversion of natively unfolded monomeric protein/peptide into misfolded β-sheet rich aggregates. (nature.com)
Pathologies and dementias of the nervous system such as Alzheimer's disease and Parkinson's disease are associated with tau proteins that have become hyperphosphorylated insoluble aggregates called neurofibrillary tangles. (wikipedia.org)
[7] Additional preclinical research indicates curcumin (and its analogs) has inhibitory and protective effects against Alzheimer's disease associated β-amyloid proteins. (greenmedinfo.com)
Turmeric contains a variety of compounds (curcumin, tetrahydrocurcumin, demethoxycurcumin and bisdemethoxycurcumin) which may strike to the root pathological cause of Alzheimer's disease by preventing β-amyloid protein formation. (greenmedinfo.com)
For example, in the case of Alzheimer's syndrome, the illness is associated to the denaturation and aggregation of the amyloid-β-protein (Aβ). (frontiersin.org)
Golgi defects enhance APP amyloidogenic processing in Alzheimer's disease. (semanticscholar.org)
A similar protein misfolding that is not infectious seems to cause Alzheimer's disease. (mad-cow.org)
DNA sequences encoding β-amyloid-related proteins associated with Alzheimer's disease are disclosed. (google.com)
Antibodies generated against the recombinant proteins or immunogenic peptides derived therefrom can be used for cerebral fluid or serum protein diagnosis of Alzheimer's disease. (google.com)
More specifically, it relates to the characterization and use of materials related to amyloid protein deposits associated with Alzheimer's disease, and to a specific DNA sequence encoding a novel protease inhibitor. (google.com)
A protein secreted with insulin travels through the bloodstream and accumulates in the brains of individuals with type 2 diabetes and dementia, in the same manner as the amyloid beta (Αβ) plaques that are associated with Alzheimer's disease, a study by researchers with the UC Davis Alzheimer's Disease Center has found. (ucdavis.edu)
The study is the first to identify deposits of the protein, called amylin, in the brains of people with Alzheimer's disease, as well as combined deposits of amylin and Aβ plaques, suggesting that amylin is a second amyloid as well as a new biomarker for age-related dementia and Alzheimer's. (ucdavis.edu)
Protein Processing in Alzheimer's Disease," Peptide Res. (patentgenius.com)
The cellular prion protein traps Alzheimer's Aβ in an oligomeric form and disassembles amyloid fibers. (bath.ac.uk)

These approaches are very useful in screening mutations or potential anti-amyloidogenic drugs for their effects on fibrillogenesis and the formation of toxic oligomers. (youtube.com)

alpha-Synuclein, a protein associated with Parkinson's disease, also binds copper, but, in this case, it potentially increases the rate at which the protein aggregates. (bath.ac.uk)
In each disease, one or more proteins self-associate into toxic oligomers that disrupt cellular function and communication, and proceed to form insoluble amyloid aggregates characterized by fibrillar morphology and cross-β structure. (springer.com)
Experimental studies of the nature of protein aggregation are unfortunately limited by the structure of aggregates and their insolubility in water. (edu.pl)
NQTrp and Cl-NQTrp significantly disrupted the pre-formed fibrillar aggregates of Tau-derived PHF6 (VQIVYK) peptide and full-length tau protein in vitro , in a dose-dependent manner as evident from ThS assay, CD spectroscopy, and TEM. (nature.com)
Under disease conditions, the formation of misfolded protein aggregates is a progressive process leading to increasing loads of amyloid deposits in AD brain 16 . (nature.com)
A specifically proposed mechanism involved in the formation of protein aggregates in s-IBM is inhibition of the ubiquitin-26S proteosome system, which is the primary degradation pathway for misfolded, unfolded, and other damaged proteins. (medscape.com)
A commonly used approach for studying protein aggregates in cells is number and brightness (N&B), a fluorescence microscopy method that is capable of measuring the apparent average number of molecules and their oligomerization (brightness) in each pixel from a series of fluorescence microscopy images. (nature.com)
Here, I offer an overview of the molecular mechanisms of both protein folding and misfolding, particularly in the formation of aggregates. (jci.org)
This Perspective first revisits some of the seminal developments in protein biochemistry that led to the idea that protein aggregates contain specific, organized, polymeric structures formed from partly structured folding intermediates by alternative, off-pathway folding steps. (jci.org)
The contributors examine the relevant biochemical attributes of proteins (e.g., solubility), the functions of normal protein aggregates (e.g., biofilm formation in bacteria), and the various heat shock proteins, chaperones, translocation machineries, proteasomes, signaling factors, and transcriptional programs involved in proteostasis. (cshlpress.com)
Desmin-related cardiomyopathy is characterized by the formation of intracellular aggregates containing CryAB and desmin that are amyloid positive, and disease can be recapitulated in transgenic mice by cardiac-specific expression of the mutant protein. (ahajournals.org)
When over-secreted, some proteins have a higher propensity to stick to one another, forming small aggregates, called oligomers, fibrils and amyloids. (ucdavis.edu)

Over 100 amyloidogenic mutations have been reported and they represent the most common cause of hereditary amyloidosis. (biocompare.com)
The three nonconservative mutations in AL-09 are located within the dimer interface, consistent with their role in the decreased stability of this amyloidogenic protein. (elsevier.com)
FAP is associated with mutations of the plasma protein transthyretin (TTR). (diva-portal.org)
ApoAI can present either as a nonhereditary form with wild-type protein deposits in atherosclerotic plaques or as a hereditary form due to germline mutations in the apoA1 gene. (medscape.com)
3 Although the initial focus centered on mutations in the intermediate filament protein desmin, it has became apparent that mutations in other proteins that interact with desmin, such as the R120G mutation in CryAB (CryAB R120G ), can phenocopy the disease. (ahajournals.org)
Recently, bi-allelic mutations in GPNMB, encoding glycoprotein (transmembrane) non-metastatic melanoma protein b have been described in ACD (Yang et al. (medworm.com)

In this chapter the molecular studies of amyloidogenic proteins like prion protein, transthyretin and human cystatin C are presented. (edu.pl)
To probe these conformational changes at atomic resolution, we have performed molecular dynamics simulations of these proteins at neutral and low pH. (pnas.org)
We have characterized the molecular changes in primary human cardiac fibroblasts (hCFs) exposed in vitro to soluble amyloidogenic cardiotoxic LCs from AL cardiomyopathy patients. (upf.edu)
The proteome alterations affect proteins involved in cytoskeletal organization, protein synthesis and quality control, mitochondrial activity and metabolism, signal transduction and molecular trafficking. (upf.edu)
Most archaea have a so-called surface layer (S-layer) cell wall structure made up of a single protein or glycoprotein with a molecular mass in the 40-200 kDa range ( Albers and Meyer, 2011 ). (frontiersin.org)
The focus then turns to cellular responses in which aggregation is prevented or reversed by molecular chaperones, molecules dedicated to providing kinetic assistance to protein folding. (jci.org)
Last year the Chicago team led by Susan Lindquist, Ph.D., professor of molecular genetics and cell biology, showed that prion-like proteins exist in yeast. (mad-cow.org)
They now show that even in the test tube, the purified yeast protein can knit together into fibers that have the same staining properties and molecular architecture as the amyloid plaques seen at autopsy in the brains of animals and humans that have died of transmissible spongiform encephalopathies. (mad-cow.org)
Structural characterization of amyloid rich in cross-β structures is crucial for unraveling the molecular basis of protein misfolding and amyloid formation associated with a wide range of human disorders. (osti.gov)
These proteins demonstrated diverse isoelectric points and molecular weights and are involved in several molecular functions, including protein binding, catalytic activity, transport, structure, and signal transduction. (mcponline.org)
They are caused by different proteins that, under certain circumstances, aggregate and become toxic as we grow older, but the molecular events underlying this process remain unclear. (plos.org)

To analyze whether the secretory pathway plays a role in the amyloidogenic processing of APP695, we constructed an APP695 hybrid containing the C-terminal sorting signal of rat TGN38. (jneurosci.org)
Reductions in Abeta may be related to shifted APP processing toward the non-amyloidogenic pathway, because alpha-secretase enzymatic activity was increased in the absence of changes in beta-secretase activity. (nih.gov)
In the non-amyloidogenic (non-plaque-forming) pathway, APP is cleaved by alpha-secretase to yield a soluble N-terminal sAPP-alpha (neuroprotective) and a membrane-bound CTF-alpha. (ebi.ac.uk)
In the amyloidogenic pathway (plaque-forming), APP is broken down by beta-secretase to yield soluble sAPP-beta and membrane-bound CTF-beta. (ebi.ac.uk)
The proteins with increased levels included proteins involved in cell growth, angiogenesis and complement pathway while proteins with decreased levels included those involved in cell adhesion, plasminogen pathway and extracellular matrix remodeling. (mdpi.com)
This session seeks submissions focused on experimental and theoretical characterization and prediction of the thermodynamics and mechanism(s) of protein folding, and the role of aggregation as an on- or off-pathway aspect of (un)folding. (confex.com)
Amyloid-β and proinflammatory cytokines utilize a prion protein-dependent pathway to activate NADPH oxidase and induce cofilin-actin rods in hippocampal neurons. (bath.ac.uk)
Methionine oxidation perturbs the structural core of the prion protein and suggests a generic misfolding pathway. (bath.ac.uk)

In this study, we address the structural basis of immunoglobulin light chain amyloidosis, which results from deposition of light chains produced by clonal plasma cells.Wecompare light chain amyloidosis protein AL-09 to its wild-type counterpart, the κI O18/O8 light chain germline. (elsevier.com)
Generally speaking, numerous proteins with varying size, shape and chemical composition are likely involved in amyloidosis [ 5 ]. (frontiersin.org)
The plasma protein transthyretin (TTR) is associated with familial amyloidosis with polyneuropathy (FAP) and senile systemic amyloidosis (SSA). (diva-portal.org)
Ando Y, Ueda M. Novel methods for detecting amyloidogenic proteins in transthyretin related amyloidosis. (medscape.com)
Successful treatment of protein-losing enteropathy due to AA amyloidosis with octreotide in a patient with rheumatoid arthritis. (medscape.com)
Retention and accumulation of this type of amyloid protein is presumed to be the main pathogenic process underlying beta-2m amyloidosis. (medscape.com)

Peptides that differ in their potential to aggregate and form fibrils will be chosen, to investigate whether the structural state of the protein is critical in its pathological effects. (europa.eu)
In addition in this chapter the MD studies of protein fibrils are presented. (edu.pl)
Upon partial acid denaturation, these proteins undergo conformational change into an amyloidogenic intermediate that can self-assemble into amyloid fibrils. (pnas.org)
Amyloid disease involves the conversion of a protein or peptide from its soluble native state into insoluble amyloid fibrils. (pnas.org)
Given that many different sequences can form amyloid fibrils of similar architecture, there may be some common structural elements of the prefibrillar amyloidogenic intermediate. (pnas.org)
Therefore, it has been widely held that the formation of amyloid fibrils involves a transition to β-sheet secondary structure in the amyloidogenic intermediate. (pnas.org)
Besides the tissue alteration caused by fibrils, clinical and experimental evidence indicates that cardiac damage is also caused by proteotoxicity of prefibrillar amyloidogenic species. (upf.edu)
In common with other misfolded and aggregated proteins, amyloid formation involves aggregation of monomers of hIAPP into oligomers, fibrils, and ultimately mature amyloid deposits. (hindawi.com)
Similarly, the Parkinson disease is associated with the formation of fibrils after α-synuclein protein denaturate, and aggregate in the so called Lewy bodies in the brain. (frontiersin.org)
Despite the origin of protein in the amyloid, the fibrils share some common biochemical and biophysical properties such as a diameter of 8-13 nm, a β-pleated sheet secondary structure packed in an ordered crystal-like way, Congo red and thioflavin binding with characteristic spectroscopic patterns and decoration of the fibrils with Serum amyloid P component and glycoseaminoglycans. (diva-portal.org)

Purification of recombinant amyloidogenic proteins can sometimes prove challenging due in part to their aggregation-prone character. (biocompare.com)
These sequences are used in producing or constructing recombinant β-amyloid core protein, β-amyloid-related proteins and. (google.com)
To that end, we have set up bacterial high cell density fermentation in bioreactors for recombinant protein production. (uni-ulm.de)

Alzheimer disease), and pharmacologic approaches to ameliorate protein conformational disorders are also included. (cshlpress.com)
A favored hypothesis postulates that a critical conformational change in the monomer (an ideal therapeutic target) in these "neurotoxic proteins" triggers the pathogenic cascade. (plos.org)
We use force spectroscopy and a novel methodology for unequivocal single-molecule identification to demonstrate a rich conformational polymorphism in the monomer of four representative neurotoxic proteins. (plos.org)

Coverage of the pathologic and pathogenic structures of amyloidogenic proteins from the pathological lesions to the evasive oligomers that are believed to be the main culprits. (springer.com)
Therefore, the alternate theory has arisen that s-IBM is a primarily degenerative disorder related to aging of the muscle, supported by the finding of abnormal, potentially pathogenic protein accumulations in myofibers. (medscape.com)
Several protein kinases are also involved in the s-IBM pathogenic cascade. (medscape.com)
The interaction of the neuronal protein α-synuclein with lipid membranes appears crucial in the context of Parkinson's disease, but the underlying mechanistic details, including the roles of different lipids in pathogenic protein aggregation and membrane disruption, remain elusive. (pnas.org)

Computational tools are being applied to solve the protein aggregation and fibrillization problem, providing insight into amyloid structures and aggregation mechanisms. (edu.pl)
Very efficient theoretical models for prediction of protein aggregation propensities from primary structures have been proposed. (edu.pl)
MD-generated α-sheet intermediate structures for four amyloidogenic proteins: transthyretin ( a ), β 2 m ( b ), D67H lysozyme ( c ), and bovine PrP ( d ). (pnas.org)
The PDBFlex database explores the intrinsic flexibility of protein structures by analyzing structural variations of the same protein across the archive. (rcsb.org)
Atomic structures of low-complexity protein segments reveal kinked β sheets that assemble networks. (alzforum.org)
Within these structures, alpha-synuclein interacts with phospholipids and proteins. (wikipedia.org)
The roles of specific subcellular structures-the endoplasmic reticulum, mitochondria, ribosomes, lysosomes, and cytoplasm-in protein quality control are covered, as is the regulation of proteostasis at the organismal level (e.g., via neuronal activity). (cshlpress.com)
Recent major advances in our understanding of modulating protein functions has led to the development of new methods and algorithms to predict and decipher how amino acid sequences shape three-dimensional structures. (nhbs.com)
Our results therefore indicate that interaction between transcriptional regulators and their target proteins in Mediator are less dependent on the primary sequences in the interaction domains but that these domains fold into similar structures upon interaction. (diva-portal.org)
Here, we use an established single-molecule manipulation technique combined with a new protein-engineering strategy to show that all these proteins can adopt a rich collection of structures (conformers) that includes a high proportion of mechanostable conformers, which are associated with toxicity and disease. (plos.org)
We also find that a known drug can block the formation of these mechanostable structures in different neurotoxic proteins. (plos.org)

The pathology is characterized by accumulations of protease- resistant isoforms of the prion protein PrP (PrPSc), neurodegeneration with progressive loss, and vacuolation of neuronal processes. (europa.eu)
RESEARCH NEWS 2018-02-16 Research News Many proteins involved in neurodegeneration have a penchant to undergo liquid phase separation, where they coalesce into highly concentrated fluid droplets. (alzforum.org)
I conducted my PhD training in the laboratory of Prof Vladimir Buchman in Cardiff University, using rodent models to understand the role of synuclein proteins to neurodegeneration and basic neuronal function. (cardiff.ac.uk)
2015. Human C9ORF72 hexanucleotide expansion reproduces RNA foci and dipeptide repeat proteins but not neurodegeneration in BAC transgenic mice . (cardiff.ac.uk)

Computational simulations constitute an "analytical tool" for obtaining and processing biological information and to make useful explanations of the physicochemical principles of amyloidogenesis, as well as to understand the role amino-acid sequences in amyloidogenic proteins. (edu.pl)
AL-Base: a visual platform analysis tool for the study of amyloidogenic immunoglobulin light chain sequences. (semanticscholar.org)
AL-Base is designed to compile and analyse amyloidogenic Ig LC sequences and to compare their predicted protein sequence and structure to non-amyloidogenic LC sequences. (semanticscholar.org)
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. (genecards.org)

The MD studies of these proteins show the first steps during amyloids formation. (edu.pl)
Hyphenation of FTIR and microcapillary systems for the separation and detection of amyloidogenic proteins such as abeta amyloids and beta-2-microglobulin. (iit.it)

The simulation systems covered a range from simple peptides (Alzheimer Aβ peptides or peptides being fragments of amyloidogenic proteins), to large proteins (transthyretin, prion protein, cystatin C, β2-microglobulin etc. (edu.pl)
Although indicating the potential importance of this part in amyloidogenic processing, these fragments also have been proposed to be intermediates of the final degradation of APP in lysosomes ( Haass and Selkoe, 1993 ). (jneurosci.org)
Brain cathepsin B but not metalloendopeptidases degrade rAPP.sup.751 with production of amyloidogenic fragments," Int. J. of Peptide & Protein Res. (patentgenius.com)

The protein kinase C (PKC) translocation state in the soluble and membrane-bound fractions and the levels of β-secretase (BACE) were also evaluated. (elsevier.com)
Open up in another window Physique 1 A plan representing the look of the inhibitor that's selective for the phosphorylation of 1 protein substrate of the multi-substrate kinase, PKC. (antibodyassay.com)
XX) on PKC, two of many protein substrates of the protein kinase). (antibodyassay.com)
Preliminary studies in our laboratory have shown mRNA for AMP-Activated Protein Kinase (AMPK) is expressed in the rat ovary in a cell-specific manner, and suggest roles of AMPK in regulating hormone production and cell viability. (calstatela.edu)
Identification of a protein kinase as an intrinsic component of rat liver coated vesicles," Biochem. (patentgenius.com)

Tau is a negative regulator of protein translation in both Drosophila and human brains, through its binding to ribosomes, which results in impaired ribosomal function, reduction of protein synthesis and altered synaptic function. (wikipedia.org)
Atypical, non-standard roles of tau are also under current investigation, such as its involvement in chromosome stability, its interaction with the cellular transcriptome, its interaction with other cytoskeletal or synaptic proteins, its involvement in myelination or in brain insulin signaling, its role in the exposure to chronic stress and in depression, etc. (wikipedia.org)
Alpha-synuclein is a neuronal protein that regulates synaptic vesicle trafficking and subsequent neurotransmitter release. (wikipedia.org)
These insights into structural consequences of α-synuclein interaction with lipid vesicles highlight the contrasting roles of different anionic lipids, which may be of mechanistic relevance for both normal protein function (e.g., synaptic vesicle binding) and dysfunction (e.g., mitochondrial membrane interaction). (pnas.org)

FPR2 is activated by an array of ligands, which include structurally unrelated lipids and peptide/proteins agonists, resulting in different intracellular responses in a ligand-specific fashion. (mdpi.com)

This allows for the easy identification of regions and types of structural flexibility present in a protein of interest. (rcsb.org)
Supported by a theoretical model to account for structural changes in scattering properties of surface-tethered lipid vesicles, the data demonstrate stepwise vesicle disruption and asymmetric membrane deformation upon α-synuclein binding to phosphatidylglycerol vesicles at protein concentrations down to 10 nM (∼100 proteins per vesicle). (pnas.org)
Structural clues suggest similarities between Fam96a and amyloidogenic proteins. (iucr.org)
Monoclonal antibodies were generated that exclusively recognize the amyloidogenic folding of TTR providing direct biochemical evidence for a structural change in amyloidogenic intermediates. (diva-portal.org)
Other topics of discussion include: design of structural elementary motifs, entire proteins, and interfaces of protein complexes, and of amyloidogenic polypeptides and amyloid inhibitors. (nhbs.com)

and (3) increased retrotranslocation of misfolded proteins into the cytoplasm for ubiquitination and subsequent proteosomal degradation. (medscape.com)
E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. (uniprot.org)
The entire life cycle of a protein-from synthesis and folding to transport and degradation-is carefully controlled by the proteostasis network. (cshlpress.com)

Moreover, retention of APP695 proteins in the endoplasmic reticulum led to neither βA4 secretion nor to processing by β-secretase in human SH-SY5Y neuroblastoma cells. (jneurosci.org)
Unfolded protein response activation reduces secretion and extracellular aggregation of amyloidogenic immunoglobulin light chain. (semanticscholar.org)
This study shows altered secretion of key proteins in NF1 derived Schwann cells. (mdpi.com)

Development of analytical techniques to characterize amyloidogenic intermediates of β2-microglobulin and 1-40 and 1-42 Abeta-peptides. (iit.it)

This network, consisting of many interconnected pathways and processes, manages protein homeostasis by dynamically responding to the needs of the cell. (cshlpress.com)
Preference will be given to presentations that provide insights of general utility, and those focused on relatively complex (un)folding pathways such as multi-domain and natively multimeric proteins. (confex.com)

At a minimal computational cost, some of these models can determine putative, aggregation-prone regions ("hot-spots") within a protein sequence. (edu.pl)
A number of these peptides are synthesized based on the sequence of known proteins, but their physiological function and their presence in vivo has to be proven. (mdpi.com)
Depolymerizing the sheaths from M. hungatei JF-1 allowed us to identify a 40.6 kDa protein (WP_011449234.1) that shares 23% sequence similarity to MspA from M. thermophila PT (ABK14853.1), indicating that they might be distant homologs. (frontiersin.org)
The MspA protein discovered here could accordingly represent a more widely found sheath protein than the MspA from M. thermophila PT, which currently has no homologs in the NCBI Reference Sequence database (RefSeq). (frontiersin.org)
The ability of the reduced and unfolded protein to spontaneously fold into its native state established that the primary amino acid sequence of a protein contains all of the information necessary for proper folding into native form, a fundamental principle for which Anfinsen received the Nobel Prize in Chemistry in 1972. (jci.org)
Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed. (uniprot.org)
4. An analog of human amyloid plaque core protein protease inhibitor wherein the amino acid corresponding to arginine at position 13 in the sequence ##STR7## is substituted with an aromatic amino acid, said analog exhibiting chymotrypsin inhibitory activity. (google.com)
Despite low sequence similarity, hMed25-ACID also interacts with the plant-specific Dreb2a transcriptional activator protein. (diva-portal.org)

The tau proteins were identified in 1975 as heat-stable proteins essential for microtubule assembly, and since then they have been characterized as intrinsically disordered proteins. (wikipedia.org)
Alpha-synuclein in solution is considered to be an intrinsically disordered protein, i.e. it lacks a single stable 3D structure. (wikipedia.org)

Evidence suggests that association of these proteins with lipid membranes is crucial for both functional and pathological roles. (pnas.org)
The interaction of proteins with lipid membranes is a ubiquitous phenomenon in biology. (frontiersin.org)
Proteins interact with cell membranes for signaling purposes, for nutrition, cell division and many other critical processes [ 1 ]. (frontiersin.org)
Proteins can refold at membranes and eventually aggregate [ 2 ]. (frontiersin.org)
As a result of multiple isoforms and cellular locales, GAPDH is able to come in contact with a variety of small molecules, proteins, membranes, etc., that play important roles in normal and pathologic cell function. (iospress.com)

The in silico simulations increase our understanding of the protein aggregation process. (edu.pl)

The complexity of proteins is itself a limiting factor in understanding the exact mechanisms by which amyloid formation occurs [ 3 ]. (frontiersin.org)
This FOA invites applications that will systematically and comprehensively characterize alpha-synuclein and amyloid-beta subspecies present in human Lewy Body Dementia (LBD) post-mortem brain tissue, identify toxic subspecies and potential mechanisms of toxicity, and characterize any interactions between the proteins that may contribute to increased toxicity and/or explain selective vulnerabilities of cells/circuits. (nih.gov)

FPR2 seems unusual, being used by both lipid and protein ligands. (mdpi.com)
In Parkinson's disease, the involved protein, α-synuclein, is thought to function in trafficking of lipid vesicles in the brain. (pnas.org)
With this approach, we discovered that α-synuclein perturbs vesicles in a stepwise and lipid-dependent fashion already at very low protein coverage. (pnas.org)

Involved in cell mobility and transcription regulation through protein-protein interactions. (abcam.com)

Other amyloidogenic disease-related proteins, such as the prion protein or α -synuclein, also exhibit similarities in the formation of amyloid channels and in the disruption of calcium homeostasis. (hindawi.com)
They also show that the formation of fibers from normal protein molecules is greatly speeded up by the presence of defective ones. (mad-cow.org)
Colocalization of galectin-3 binding sites with desmosomal proteins may indicate a role for this endogenous lectin in the formation of intercellular contacts of the desmosomal type. (biomedsearch.com)

As this antibody is specific for the soluble oligomeric species of many amyloidogenic proteins and peptides [Aβ42, α-synuclein, islet amyloid polypeptide, poly( l -glutamine), lysozyme, human insulin, and PrP peptide 106-126], they may have a common structure. (pnas.org)

As is the case for other chaperones and the small heat shock proteins, CryAB can bind to unfolded proteins and prevent their denaturation and aggregation. (ahajournals.org)

Amongst tau and Aβ, the role of tau in AD pathology has gained significant importance as the severity of AD is better correlated with the deposition of Neurofibrillary Tangles (NFTs) of the tau protein than senile plaques of Aβ 4 . (nature.com)
FAP is characterized by the systemic deposition of amyloidogenic variants of the transthyretin protein , especially in the peripheral nervous system , causing a progressive sensory and motor polyneuropathy . (wikipedia.org)
The present study is a part of our ongoing effort to detect protein expression alterations in human frontal cortex as Lewy body deposition spreads from the brainstem to the limbic system and eventually to the isocortex with progression of PD ( 2 , 16 , 17 ). (mcponline.org)

Formyl-peptide receptors 1, 2 and 3 (FPR1, FPR2 and FPR3) form a subgroup of receptors linked to inhibitory G-proteins (G i ). (mdpi.com)
Is the inhibitory effect of GM1 on Ab oligomerisation generic to other amyloidogenic proteins? (cas.cz)

Amyloid beta A4 protein (also known as APP or A4) consists of a large N-terminal extracellular region containing heparin-binding and copper-binding sites, a short hydrophobic transmembrane domain, and a short C-terminal intracellular domain. (ebi.ac.uk)

Tau proteins interact with tubulin to stabilize microtubules and promote tubulin assembly into microtubules. (wikipedia.org)
In fact, the amylin looks like the amyloid beta protein, and they both interact. (ucdavis.edu)

protein in endoplasmic reticulum and golgi," Biochem. (patentgenius.com)

The number of papers published on simulations of amyloidogenic proteins has increased rapidly during the last decade. (edu.pl)

Mechanistic studies of both productive protein folding and misfolding/aggregation have been considerably advanced by the ability to observe these reactions in vitro with purified proteins. (jci.org)

The tau proteins (or τ proteins, after the Greek letter with that name) are a group of six highly soluble protein isoforms produced by alternative splicing from the gene MAPT (microtubule-associated protein tau). (wikipedia.org)
It has been shown that alpha-synuclein significantly interacts with tubulin, and that alpha-synuclein may have activity as a potential microtubule-associated protein, like tau. (wikipedia.org)

In human brain, tau proteins constitute a family of six isoforms with a range of 352-441 amino acids. (wikipedia.org)

In humans, the MAPT gene for encoding tau protein is located on chromosome 17q21, containing 16 exons. (wikipedia.org)
Alpha-synuclein is a protein that, in humans, is encoded by the SNCA gene. (wikipedia.org)
Independent of these studies, our group established that HspB5 was both the most prominent protein in plaques of MS patients ( 10 ) and the gene whose expression was most induced in the MS plaques compared with normal tissue ( 11 ). (jimmunol.org)
CST3 (Cystatin C) is a Protein Coding gene. (genecards.org)
Gene Ontology (GO) annotations related to this gene include identical protein binding and endopeptidase inhibitor activity . (genecards.org)

Search for conserved amino acid residues of the α-crystallin proteins of vertebrates. (nih.gov)
Aromatic amino acids occur more often in [Formula: see text]-crystallins from vertebrates than on the average in proteins among 17 animal proteomes. (nih.gov)
The human alpha-synuclein protein is made of 140 amino acids. (wikipedia.org)
The majority form of the protein, and the one most investigated, is the full-length protein of 140 amino acids. (wikipedia.org)

It is predominantly a neuronal protein, but can also be found in the neuroglial cells. (wikipedia.org)

Tau interacts specifically with several ribosomal proteins, including the crucial regulator of translation rpS6. (wikipedia.org)

Insulin is a globular protein, with a relatively simple structure, and small size. (frontiersin.org)

Alpha-synuclein is a synuclein protein of unknown function primarily found in neural tissue, making up as much as 1% of all proteins in the cytosol of brain cells. (wikipedia.org)

To assess the generalizability of our results we explored the effects of multiple LCs on hCF viability and on levels of a subset of cellular proteins. (upf.edu)
Similar to actions in Alzheimer disease , myofibers in s-IBM accumulate amyloid-β (Aβ), phosphorylated tau (p-tau), apolipoprotein E, presenilin-1, the normal cellular isoform of prion protein (PrP c ), and many other characteristic proteins. (medscape.com)
Protein dimerization and oligomerization are essential to most cellular functions, yet measurement of the size of these oligomers in live cells, especially when their size changes over time and space, remains a challenge. (nature.com)
Fluorescent proteins (FPs) have great utility in identifying specific cell populations and in studying cellular dynamics in the mouse. (biologists.org)
Unlike normal misfolded proteins, the misfolding nature of prions is associated with their ability to actively sequester their counterparts (cellular functional proteins) and induce them to adopt a similar misfolded conformation, thus rendering them nonfunctional ( 6 ). (asm.org)
For 15 y, α B-crystallin (heat shock protein [Hsp] B5) has been labeled an autoantigen in multiple sclerosis (MS) based on humoral and cellular responses found in humans and animal models. (jimmunol.org)
However, in several of the reports, cellular and humoral responses against the protein also were seen in normal plasma ( 3 , 4 , 8 ). (jimmunol.org)

Anatomy1

Organisms1

Diseases7

Chemicals and Drugs24

Analytical, Diagnostic and Therapeutic Techniques and Equipment7

Psychiatry and Psychology1

Phenomena and Processes18

Information Science2

Evolution of Nova-dependent splicing regulation in the brain. (1/71)

Amyloid-beta oligomers impair fear conditioned memory in a calcineurin-dependent fashion in mice. (2/71)

Mechanisms of AD neurodegeneration may be independent of Abeta and its derivatives. (3/71)

Glutamate carboxypeptidase II: an amyloid peptide-degrading enzyme with physiological function in the brain. (4/71)

The heparan sulfate motif (GlcNS6S-IdoA2S)3, common in heparin, has a strict topography and is involved in cell behavior and disease. (5/71)

Report of the task force on designing clinical trials in early (predementia) AD. (6/71)

Sulfated glycosaminoglycans accelerate transthyretin amyloidogenesis by quaternary structural conversion. (7/71)

Novel CHST6 gene mutations in 2 unrelated cases of macular corneal dystrophy. (8/71)

Amyloidogenic Proteins

Amyloid

Amyloidosis

Prealbumin

Islet Amyloid Polypeptide

Congo Red

Amyloid beta-Peptides

Protein Folding

beta 2-Microglobulin

Prions

Protein Structure, Secondary

alpha-Synuclein

Protein Structure, Quaternary

Immunoglobulin Light Chains

Circular Dichroism

Protein Multimerization

Protein Conformation

Neurodegenerative Diseases

Microscopy, Electron, Transmission

Peptide Fragments

Alzheimer Disease

Amyloid beta-Protein Precursor

Amyloidosis, Familial

Spectroscopy, Fourier Transform Infrared

Models, Molecular

Amino Acid Sequence

Peptides

Amyloid Precursor Protein Secretases

Molecular Sequence Data

Hydrogen-Ion Concentration

Amyloid Neuropathies

Protein Binding

Kinetics

Aspartic Acid Endopeptidases

Plaque, Amyloid

Amyloid Neuropathies, Familial

Microscopy, Atomic Force

Bence Jones Protein

Insulysin

Thiazoles

Neurofibrils

Hydrophobic and Hydrophilic Interactions

Protein Processing, Post-Translational

Muramidase

Protein Stability

Serum Amyloid A Protein

Hydrogen Bonding

Endopeptidases

Protein Denaturation

Protein Structure, Tertiary

Mutation

Peptide Termination Factors

Protein Unfolding

Presenilin-1

Presenilin-2

Thermodynamics

Mice, Transgenic

Evolution of Nova-dependent splicing regulation in the brain. (1/71)

Amyloid-beta oligomers impair fear conditioned memory in a calcineurin-dependent fashion in mice. (2/71)

Mechanisms of AD neurodegeneration may be independent of Abeta and its derivatives. (3/71)

Glutamate carboxypeptidase II: an amyloid peptide-degrading enzyme with physiological function in the brain. (4/71)

The heparan sulfate motif (GlcNS6S-IdoA2S)3, common in heparin, has a strict topography and is involved in cell behavior and disease. (5/71)

Report of the task force on designing clinical trials in early (predementia) AD. (6/71)

Sulfated glycosaminoglycans accelerate transthyretin amyloidogenesis by quaternary structural conversion. (7/71)

Novel CHST6 gene mutations in 2 unrelated cases of macular corneal dystrophy. (8/71)

Identification of Patients with Preeclampsia by Measuring Fluorescence of an Amyloid-Binding Aryl Cyano Amide in Human Urine...

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Fibrillary and Immunotactoid Glomerulopathies - Genitourinary Disorders - Merck Manuals Professional Edition

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