A group of amylolytic enzymes that cleave starch, glycogen, and related alpha-1,4-glucans. (Stedman, 25th ed) EC 3.2.1.-.
A nodular organ in the ABDOMEN that contains a mixture of ENDOCRINE GLANDS and EXOCRINE GLANDS. The small endocrine portion consists of the ISLETS OF LANGERHANS secreting a number of hormones into the blood stream. The large exocrine portion (EXOCRINE PANCREAS) is a compound acinar gland that secretes several digestive enzymes into the pancreatic ductal system that empties into the DUODENUM.
The largest of the three pairs of SALIVARY GLANDS. They lie on the sides of the FACE immediately below and in front of the EAR.
Enzymes that catalyze the endohydrolysis of 1,4-alpha-glycosidic linkages in STARCH; GLYCOGEN; and related POLYSACCHARIDES and OLIGOSACCHARIDES containing 3 or more 1,4-alpha-linked D-glucose units.
INFLAMMATION of the PANCREAS. Pancreatitis is classified as acute unless there are computed tomographic or endoscopic retrograde cholangiopancreatographic findings of CHRONIC PANCREATITIS (International Symposium on Acute Pancreatitis, Atlanta, 1992). The two most common forms of acute pancreatitis are ALCOHOLIC PANCREATITIS and gallstone pancreatitis.
A specific decapeptide obtained from the skin of Hila caerulea, an Australian amphibian. Caerulein is similar in action and composition to CHOLECYSTOKININ. It stimulates gastric, biliary, and pancreatic secretion; and certain smooth muscle. It is used in paralytic ileus and as diagnostic aid in pancreatic malfunction.
The clear, viscous fluid secreted by the SALIVARY GLANDS and mucous glands of the mouth. It contains MUCINS, water, organic salts, and ptylin.
An enzyme that hydrolyzes 1,6-alpha-glucosidic branch linkages in glycogen, amylopectin, and their beta-limit dextrins. It is distinguished from pullulanase (EC 3.2.1.41) by its inability to attack pullulan and by the feeble action of alpha-limit dextrins. It is distinguished from amylopectin 6-glucanohydrolase (EC 3.2.1.69) by its action on glycogen. With EC 3.2.1.69, it produces the activity called "debranching enzyme". EC 3.2.1.68.
A peptide, of about 33 amino acids, secreted by the upper INTESTINAL MUCOSA and also found in the central nervous system. It causes gallbladder contraction, release of pancreatic exocrine (or digestive) enzymes, and affects other gastrointestinal functions. Cholecystokinin may be the mediator of satiety.
The fluid containing digestive enzymes secreted by the pancreas in response to food in the duodenum.
An enzyme of the hydrolase class that catalyzes the reaction of triacylglycerol and water to yield diacylglycerol and a fatty acid anion. It is produced by glands on the tongue and by the pancreas and initiates the digestion of dietary fats. (From Dorland, 27th ed) EC 3.1.1.3.
Any of a group of polysaccharides of the general formula (C6-H10-O5)n, composed of a long-chain polymer of glucose in the form of amylose and amylopectin. It is the chief storage form of energy reserve (carbohydrates) in plants.
An octapeptide hormone present in the intestine and brain. When secreted from the gastric mucosa, it stimulates the release of bile from the gallbladder and digestive enzymes from the pancreas.
A subclass of alpha-amylase ISOENZYMES that are secreted into SALIVA.
The inactive proenzyme of trypsin secreted by the pancreas, activated in the duodenum via cleavage by enteropeptidase. (Stedman, 25th ed)
A condition with abnormally elevated level of AMYLASES in the serum. Hyperamylasemia due to PANCREATITIS or other causes may be differentiated by identifying the amylase isoenzymes.
The major component (about 80%) of the PANCREAS composed of acinar functional units of tubular and spherical cells. The acinar cells synthesize and secrete several digestive enzymes such as TRYPSINOGEN; LIPASE; AMYLASE; and RIBONUCLEASE. Secretion from the exocrine pancreas drains into the pancreatic ductal system and empties into the DUODENUM.
Proteins and peptides found in SALIVA and the SALIVARY GLANDS. Some salivary proteins such as ALPHA-AMYLASES are enzymes, but their composition varies in different individuals.
The amount of a substance secreted by cells or by a specific organ or organism over a given period of time; usually applies to those substances which are formed by glandular tissues and are released by them into biological fluids, e.g., secretory rate of corticosteroids by the adrenal cortex, secretory rate of gastric acid by the gastric mucosa.
A peptide hormone of about 27 amino acids from the duodenal mucosa that activates pancreatic secretion and lowers the blood sugar level. (USAN and the USP Dictionary of Drug Names, 1994, p597)
A dextrodisaccharide from malt and starch. It is used as a sweetening agent and fermentable intermediate in brewing. (Grant & Hackh's Chemical Dictionary, 5th ed)
Disease having a short and relatively severe course.
Pathological processes of the PANCREAS.
Analyses for a specific enzyme activity, or of the level of a specific enzyme that is used to assess health and disease risk, for early detection of disease or disease prediction, diagnosis, and change in disease status.
A subclass of alpha-amylase ISOENZYMES that are secreted into PANCREATIC JUICE.
Oligosaccharides containing three monosaccharide units linked by glycosidic bonds.
A slowly hydrolyzed CHOLINERGIC AGONIST that acts at both MUSCARINIC RECEPTORS and NICOTINIC RECEPTORS.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
A syndrome characterized by growth retardation, severe MENTAL RETARDATION, short stature, a low-pitched growling cry, brachycephaly, low-set ears, webbed neck, carp mouth, depressed nasal bridge, bushy eyebrows meeting at the midline, hirsutism, and malformations of the hands. The condition may occur sporadically or be associated with an autosomal dominant pattern of inheritance or duplication of the long arm of chromosome 3. (Menkes, Textbook of Child Neurology, 5th ed, p231)
An unbranched glucan in starch.
NATIONAL LIBRARY OF MEDICINE service for health professionals and consumers. It links extensive information from the National Institutes of Health and other reviewed sources of information on specific diseases and conditions.
An enzyme that catalyzes the endohydrolysis of 1,6-alpha-glucosidic linkages in isomaltose and dextrins produced from starch and glycogen by ALPHA-AMYLASES. EC 3.2.1.10.
Messages between computer users via COMPUTER COMMUNICATION NETWORKS. This feature duplicates most of the features of paper mail, such as forwarding, multiple copies, and attachments of images and other file types, but with a speed advantage. The term also refers to an individual message sent in this way.
Mechanical food dispensing machines.
The guidelines and policy statements set forth by the editor(s) or editorial board of a publication.
The profession of writing. Also the identity of the writer as the creator of a literary production.
A publication issued at stated, more or less regular, intervals.
The functions and activities carried out by the U.S. Postal Service, foreign postal services, and private postal services such as Federal Express.
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
A subspecialty of internal medicine concerned with the study of the physiology and diseases of the digestive system and related structures (esophagus, liver, gallbladder, and pancreas).
Sensation of discomfort, distress, or agony in the abdominal region.
Aromatic substances added to food before or after cooking to enhance its flavor. These are usually of vegetable origin.
Instruments for the visual examination of interior structures of the body. There are rigid endoscopes and flexible fiberoptic endoscopes for various types of viewing in ENDOSCOPY.
Lists of words, usually in alphabetical order, giving information about form, pronunciation, etymology, grammar, and meaning.
The addition of descriptive information about the function or structure of a molecular sequence to its MOLECULAR SEQUENCE DATA record.
Databases devoted to knowledge about specific genes and gene products.
Personal names, given or surname, as cultural characteristics, as ethnological or religious patterns, as indications of the geographic distribution of families and inbreeding, etc. Analysis of isonymy, the quality of having the same or similar names, is useful in the study of population genetics. NAMES is used also for the history of names or name changes of corporate bodies, such as medical societies, universities, hospitals, government agencies, etc.
The relative equivalency in the efficacy of different modes of treatment of a disease, most often used to compare the efficacy of different pharmaceuticals to treat a given disease.
Activity involved in transfer of goods from producer to consumer or in the exchange of services.
The act or practice of calling public attention to a product, service, need, etc., especially by paid announcements in newspapers, magazines, on radio, or on television. (Random House Unabridged Dictionary, 2d ed)
Works about lists of drugs or collections of recipes, formulas, and prescriptions for the compounding of medicinal preparations. Formularies differ from PHARMACOPOEIAS in that they are less complete, lacking full descriptions of the drugs, their formulations, analytic composition, chemical properties, etc. In hospitals, formularies list all drugs commonly stocked in the hospital pharmacy.

The structure of a glycopeptide (GP-II) isolated from Rhizopus saccharogenic amylase. (1/1858)

Mild alkaline treatment of glycopeptide (GP-II) resulted in the loss of 1 mole of serine and 5 moles of threonine per mole of GP-II, suggesting the presence of O-glycosyl bonds between 1 serine and 5 threonine residues and carbohydrate chains. Treatment of GP-II with alkaline borohydride released only disaccharide. Methylation studies of the carbohydrate moiety gave 2,3,4,6-tetra-O-methyl and 2,4,6-tri-O-methyl derivatives of mannose in a ratio of approximately 1:1. In addition, one step of Smith degradation resulted in the loss of about 6 residues of mannose per mole of GP-II. Moreover, alpha-mannosidase [EC 3.2.1.24] liberated about 6 residles of mannose per mole of GP-II. On the basis of these data, the structure of the carbohydrate moiety of GP-II was confirmed to be 3-O-alpha-mannosylmannose. The amino- and carboxyl-terminal amino acids of GP-II were determined to be threonine and serine, respectively. On reductive cleavage of N-proline bonds with metallic sodium in liquid ammonia, 2 moles of alanine per mole of GP-II were lost. From the compositions of three fragments isolated from the reductive cleavage products, the amino acid sequence of the peptide portion of GP-II was determined. Based on these data, a probable structure was proposed for GP-II.  (+info)

Purification of gibberellic acid-induced lysosomes from wheat aleurone cells. (2/1858)

Using isopycnic density gradient centrifugation, lysosomes were concentrated in a single region of a sucrose-Ficoll gradient (p = 1-10 g cm-3), well separated from most other cell organelles. Gibberellic acid-induced lysosomes were found to be rich in alpha-amylase and protease but not ribonuclease. The lysosomal band also contained a majority of the NADH2-cytochrome c reductase, a marker enzyme for endoplasmic reticulum, found in the gradient. Examination of electron micrographs revealed that a purified band of lyosomes contained at least 3 vesicle types, ranging in size from 0-1 to 0-5 mum. The significance of these findings to proposed mechanisms of action of gibberellic acid is discussed.  (+info)

Detection of viruses and body fluids which may contain viruses in the domestic environment. (3/1858)

The domestic environment was investigated for the presence of viruses and body fluids that may contain viruses. A range of surfaces in 39 homes (17 visited on 2 occasions) were sampled by swabbing and analysed using cell culture, reverse transcription polymerase chain reaction for enteroviral RNA, haemoglobin as a marker for blood, amylase as an indicator of urine, saliva and sweat, and protein as an indicator of general hygiene. Haemoglobin was found on 1.9% of surfaces sampled and of the positive samples 30% were from articles frequently handled. Amylase (> 5 U/l) was found in 29.3% of samples tested. Protein was found in 97.8% of samples tested. Enteroviral RNA, indicating the presence of virus, was detected in 3 out of 448 samples tested; they were from a tap handle, telephone handpiece and a toilet bowl. No viruses were isolated in cell culture, however significant problems were encountered with bacterial and fungal contamination. This work demonstrates that only testing environmental samples for bacteria and ATP may not give a total view of the microbiological problem in the home. A range of test methods is useful to gain a broad view of the problems of hygiene in the home and to allow comparative studies of specific areas such as the kitchen and bathroom.  (+info)

Underestimation of acute pancreatitis: patients with only a small increase in amylase/lipase levels can also have or develop severe acute pancreatitis. (4/1858)

BACKGROUND: In most treatment studies on acute pancreatitis, pancreatologists base their diagnosis on amylase/lipase levels more than three times above the upper limit of normal (>3n) and thus exclude patients with smaller enzyme level increases. The recommendations derived from the results of treatment studies do not take into account such patients. Non-pancreatologists frequently believe that only patients with high enzyme levels have a serious prognosis. AIMS: To question the assumption that high enzyme levels indicate severe, and conversely low enzyme levels indicate mild, acute pancreatitis. PATIENTS/METHODS: This retrospective study includes 284 consecutive patients with a first attack of acute pancreatitis. The cause was biliary in 114 (40%) patients, alcoholism in 83 (29%), other in 21 (7%), and unknown in 66 (23%). Patients were divided into two groups according to their serum enzyme levels (amylase: 3n, n = 196; lipase: 3n, n = 233). Renal impairment, indication for dialysis and artificial ventilation, development of pseudocysts, necessity for surgery, and mortality were taken as parameters of severity. RESULTS: The incidence of severity was the same for both the 3n groups. CONCLUSIONS: The severity of acute pancreatitis is independent of the elevation in serum amylase/lipase level (3n) on admission. Patients with only a slight increase can also have or develop severe acute pancreatitis. Patients with +info)

Genetic regulation of tissue-specific expression of amylase structural genes in Drosophila melanogaster. (5/1858)

Laboratory strains of Drosophila melanogaster were screened for spatial variations in adult midgut alpha-amylase (1,4-alpha-D-glucan glucanohydrolase, EC 3.2.1.1) expression. No strain-specific differences were found anteriorly, but three patterns of activity were discerned in the posterior midgut: A, activity throughout most of the region; B, activity in the anterior part of the region; and C, little or no activity. Alleles of a control gene, map, are responsible for this tissue-specific regulation of activity; e.g., mapA homozygotes produce the A pattern and mapC homozygotes the C pattern. The map locus was placed at 2--80 +/- on the genetic map of chromosome 2R, about two crossover units distal to the Amy structural gene region for alpha-amylase. Electrophoretic studies showed that mapA is trans acting in mapA/mapC flies, allowing expression of amylase isozymes coded for by genes on the opposite chromosome. The map gene behaves as a temporal gene that is clearly separable from the tightly linked, duplicated Amy structural genes.  (+info)

Modes of action of acarbose hydrolysis and transglycosylation catalyzed by a thermostable maltogenic amylase, the gene for which was cloned from a Thermus strain. (6/1858)

A maltogenic amylase gene was cloned in Escherichia coli from a gram-negative thermophilic bacterium, Thermus strain IM6501. The gene encoded an enzyme (ThMA) with a molecular mass of 68 kDa which was expressed by the expression vector p6xHis119. The optimal temperature of ThMA was 60 degrees C, which was higher than those of other maltogenic amylases reported so far. Thermal inactivation kinetic analysis of ThMA indicated that it was stabilized in the presence of 10 mM EDTA. ThMA harbored both hydrolysis and transglycosylation activities. It hydrolyzed beta-cyclodextrin and starch mainly to maltose and pullulan to panose. ThMA not only hydrolyzed acarbose, an amylase inhibitor, to glucose and pseudotrisaccharide (PTS) but also transferred PTS to 17 sugar acceptors, including glucose, fructose, maltose, cellobiose, etc. Structural analysis of acarbose transfer products by using methylation, thin-layer chromatography, high-performance ion chromatography, and nuclear magnetic resonance indicated that PTS was transferred primarily to the C-6 of the acceptors and at lower degrees to the C-3 and/or C-4. The transglycosylation of sugar to methyl-alpha-D-glucopyranoside by forming an alpha-(1,3)-glycosidic linkage was demonstrated for the first time by using acarbose and ThMA. Kinetic analysis of the acarbose transfer products showed that the C-4 transfer product formed most rapidly but readily hydrolyzed, while the C-6 transfer product was stable and accumulated in the reaction mixture as the main product.  (+info)

Ultrastructural analysis of some functional aspects of Xenopus laevis pancreas during development and metamorphosis. (7/1858)

Morphological studies using both light and electron microscope were carried out with the aim of characterizing cells present in the larval and adult pancreas of Xenopus laevis. The following cell types have been seen: (1) exocrine cells, with a very well developed r.e.r. (rough endoplasmic reticulum), well defined Golgi complexes and numerous large secretory granules (A cells); (2) cells without either r.e.r. or secretory granules but with a large number of well developed mitochondria (B cells); (3) endocrine cells often clustered in the typical islets and with small membrane-coated granules showing a very dense central core surrounded by a light halo (C cells). During development, the aspect is seen to change from an unorganized tissue in which the acinar structures are still not clearly visible (stage 42), to a more organized form in which the exocrine cells (A cells) are seen to be arranged around the lumen of the acinus together with some B cells. At the stages 54-56, an increasing number of acini surrounded both by A and B cells was observed. At about stage 61, large quantities of necrotic cells were seen and it became more difficult to individualize the acinar organization found in the preceding stages. Finally, there are no necrotic cells in the adult but only A, B cells which are organized in well developed acinar structures and C cells. The investigation also included a study of some pancreatic enzymes (lipase and amylase) synthesized during larval life. Lipase activity shows a peak at stage 54-56 in which the most well organized tissue of the entire larval life was observed. The activity then decreases, reaching a minimum at stage 66, after which it rapidly rises. Maximum amylase activity occurs at stage 51 after which there is a decrease, to a minimum at stage 66. The activity then remains at constant level.  (+info)

A monoclonal anti-interleukin 8 antibody (WS-4) inhibits cytokine response and acute lung injury in experimental severe acute necrotising pancreatitis in rabbits. (8/1858)

BACKGROUND: Interleukin 8 (IL-8) has recently been proposed to have an important role in mediating the development of the systemic sequelae associated with severe acute pancreatitis. AIMS: To define the role of IL-8 in acute pancreatitis by neutralising its effects with a monoclonal anti-IL-8 antibody (WS-4), in a rabbit model of severe acute pancreatitis. METHODS: Acute pancreatitis was induced by retrograde injection of 5% chenodeoxycholic acid into the pancreatic duct and duct ligation. Twenty rabbits were divided equally into two groups: acute pancreatitis controls received physiological saline and the treated group received WS-4, 30 minutes before induction of acute pancreatitis. RESULTS: Pretreatment of animals with WS-4 resulted in significant down regulation of serum IL-8 and tumour necrosis factor alpha (TNF-alpha) from three to six hours after induction of acute pancreatitis (p = 0.011 and 0.047 for IL-8 and 0.033 and 0.022 for TNF-alpha, respectively). In addition, a significant reduction in the CD11b and CD18 positive cells and the amount of interstitial neutrophil infiltration in the lungs from WS-4 treated animals was seen. In contrast, WS-4 did not alter the amount of pancreatic necrosis and the serum concentrations of amylase, lipase, calcium, and glucose. CONCLUSION: WS-4 cannot change the amount of pancreatic necrosis induced by injection of 5% bile acid, but does reduce the acute lung injury, presumably through inhibition of circulating IL-8 and TNF-alpha, and CD11b/CD18 in lung tissue. Therefore, a role of IL-8 in the progression of acute pancreatitis and the development of its systemic complications is suggested.  (+info)

Alpha-amylase is widespread among living organisms. Salivary Amylase and Other Enzymes in Saliva, Regina Bailey is a board-certified registered nurse, science writer and educator. In humans, amylases are produced in the salivary glands and the pancreas. Salivary amylase is the primary enzyme in saliva. Salivary amylase breaks starch into a disaccharide called maltose. Salivary amylase is the primary enzyme in saliva. Learn more. As a group, kallikreins are enzymes that take high molecular weight (HMW) compounds, like kininogen, and cleave them to smaller units. Salivary kallikrein breaks down kininogen into bradykinin, a vasodilator. Like amylase, it helps with the digestion process. Amylases are common in plants, particularly in association with the starch stores of seeds and underground perennating organs such as rhizomes, tubers and taproots. The α-amylases occur in animals and include pancreatic and salivary amylase; the β-amylases occur in higher plants. Salivary amylase breaks down ...
The ratio between amylase and creatinine in serum and urine. Also referred as fractional excreted of amylase. ALCR is calculated as ...
Genetic stability of amylase activity after serial subcultivation experiments with amylolytic ruminalStreptococcus bovis strains was investigated. Two strains Amy+ and Amy− were obtained. Loss of amylase activity connected with the loss of plasmid DNA was not found in these strains. The presence of the gene responible for the amylase activity in the chromosome of these strains was revealed by hybridization of the α-amylase gene on pJK108 against chromosomal DNA ofS. bovis andBacillus subtilis after a complete restriction withEcoRI.
The amylases are a group of hydrolases that split complex carbohydrates constituted of I D glucose units. The two recognised types of amylases are ? amylase (e.g. plant and bacterial exoamylase) and human I amylases which can attack the I 1, 4 linkage anywhere along a polyglucan chain.. Human I amylase consists of two major isoenzymes, pancreatic and salivary, which are encoded by two different genes. Pancreatic amylase is synthesised only in pancreatic tissue by acinar cells. Amylase measurements are used primarily for the diagnosis and treatment of pancreatitis (inflammation of the pancreas). The evaluation of the pancreatic isoamylase may have a greater clinical specificity for the diagnosis of pancreatic disorders than total amylase assessment. ...
The major sources of amylase are the pancreas and the salivary glands. The most common cause of elevation of serum amylase is inflammation of the pancreas (pancreatitis). In acute pancreatitis, serum amylase begins to rise within 6-24 hours, remains elevated for a few days and returns to normal in 3-7 days. Other causes of elevated serum amylase are inflammation of salivary glands (mumps), biliary tract disease and bowel obstruction. Elevated serum amylase can also be seen with drugs (e.g., morphine) which constrict the pancreatic duct sphincter preventing excretion of amylase into the intestine ...
Introduction. Kiu Yi, IP 13M The effect of temperature on amylase activity Introduction The purpose of this experiment is to investigate if temperature will affect the amount of starch broken down as enzyme activity can change by different temperature. This is because as temperature rises the rate of chemical reactions increases due to the temperature increases the rate of the molecules motion. More interactions will be existed between an enzyme and its substrate. The enzyme used in this lab exercise is amylase, which is commonly found in saliva and germinating seeds, catalyzes the breakdown of starch. It also reacts quickly when heat is present during the process of it reaction. However, if the temperature is higher than the optimum point, enzymes can be denatured and they can no longer bind to a substrate and catalyze reactions. My hypothesis is therefore the amylase activity would increase as the temperature rise, until a certain high temperature at which the amylase would denature and be ...
The polymorphic patterns of human salivary amylase of a large number of individuals of Caucasian origin were determined by using isoelectric focusing and polyacrylamide gel electrophoresis. Nine diffe
TY - JOUR. T1 - Pancreatic islet-acinar cell interaction. T2 - Amylase messenger RNA levels are determined by insulin. AU - Korc, Murray. AU - Owerbach, David. AU - Quinto, Carmen. AU - Rutter, William J.. PY - 1981/1/1. Y1 - 1981/1/1. N2 - Pancreatic amylase messenger RNA progressively decreases in rats rendered diabetic with streptozotocin. Insulin reverses this effect, inducing a selective increase in amylase messenger RNA in the pancreas. Parotid amylase messenger RNA is not significantly affected by either diabetes or insulin.. AB - Pancreatic amylase messenger RNA progressively decreases in rats rendered diabetic with streptozotocin. Insulin reverses this effect, inducing a selective increase in amylase messenger RNA in the pancreas. Parotid amylase messenger RNA is not significantly affected by either diabetes or insulin.. UR - http://www.scopus.com/inward/record.url?scp=0019795914&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0019795914&partnerID=8YFLogxK. U2 - ...
Changes in rheological properties and amylase activities occurring in trifoliate yam, Dioscorea dumetorum, starch after harvest were investigated. Trifoliate yam tubers were harvested and stored under tropical ambient (28°C) and cold room conditions (4°C) for 12, 24 and 36 h. The D. dumetorum starches were extracted from the tubers under study and samples were evaluated for changes in their rheological properties (paste characteristics) during storage and to study the action of amylases on D. dumetorum starch after harvesting. The post-harvest activities of α and β-amylases were also studied to evaluate their rate of action on D. dumetorum starch. Storage caused decreases in the rheological properties (paste characteristics) of the tubers within 36 h of harvest. Similarly, α and β-amylase activities in the tubers more than doubled within 24 h after harvesting. Blanching, however, effectively decreased the action of amylases on D. dumetorum starch during storage, with subsequential increase ...
TY - JOUR. T1 - Structure of a family of rat amylase genes. AU - MacDonald, Raymond J.. AU - Crerar, Michael M.. AU - Swain, William F.. AU - Pictet, Raymond L.. AU - Thomas, Gilles. AU - Rutter, William J.. PY - 1980/12/1. Y1 - 1980/12/1. N2 - The sequences of two cloned rat pancreatic amylase cDNAs comprising 95% of the mRNA sequence are reported. Analysis of cloned rat genomic DNA fragments using cloned cDNA probes indicates that the rat genome contains multiple closely related amylase genes in which the cDNA sequences are distributed within a region 9 kilobases in length and are interrupted by at least seven intervening sequences.. AB - The sequences of two cloned rat pancreatic amylase cDNAs comprising 95% of the mRNA sequence are reported. Analysis of cloned rat genomic DNA fragments using cloned cDNA probes indicates that the rat genome contains multiple closely related amylase genes in which the cDNA sequences are distributed within a region 9 kilobases in length and are interrupted by ...
This is one of the Enzymology protocol on Effect of Temperature on Amylase activity (Enzymology Protocol). Download Salivary amylase activity assay Protocol
Cytosolic free calcium concentrations ([Ca2+]i) and amylase secretion were measured in isolated rat pancreatic acini loaded with the intracellularly trapped fluorescent indicator quin2. Both caerulein and carbamoylcholine caused a rapid increase in [Ca2+]i, with a maximal 3-fold increase at 10(-9) M-caerulein and 10(-4) M-carbamoylcholine. However, caerulein (10(-12) M and 10(-11) M) as well as carbamoylcholine (10(-7) M) caused a significant stimulation of amylase release, while not inducing any detectable rise in [Ca2+]i. Changes in [Ca2+]i after addition of either secretagogue were transient and did not last more than 2-3 min. By contrast, when amylase secretion was monitored as a function of time, two distinct secretory phases could be observed upon addition of either carbamoylcholine (10(-5) M) or caerulein (10(-10) M). An initial, rapid phase (0-5 min) which caused a 6-7-fold increase above basal, followed by a sustained (5-30 min), but less marked, secretory rate (2-3-fold above basal). ...
How to Use It Depending on the potency of the amylase inhibitors , typical intake is 1,500 to 6,000 mg before meals. Where to Find It Amylase inhibitors can be extracted from several types of plants, especially those in the legume family. Currently available Amylase inhibitors are extracted from either white kidney bean...
Amylase test, 5x100 tablets [P1302] - Equl Amylase Test The amylase assay Equl Amylase Test is used in a variety of fields for precise determination of α-amylase activity. The presence of the α-amylase enzyme in for instance saliva, food and honey makes Equl tablets useful for forensic saliva tests, diastase tests in honey or amylase tests in
Amylase Tests: Definition Amylase is a digestive enzyme made primarily by the pancreas and salivary glands. Enzymes are substances made and used by the body to trigger specific chemical reactions. The primary function of the enzyme amylase is to break down starches in food so that they can be used by the body. Amylase testing is usually done ... Blood test results reveal Blood disorders in Blood tests and also with a Blood test with Rare Blood types. Good Blood tests make possible state-of-the-art lab procedures that can be provided directly to the public in private and these Blood tests can be provided affordably. Most guidelines now suggest an amylase concentration 2-4 times the upper limit of normal is optimal for diagnostic accuracy, but this may reduce the sensitivity of the test to as low as 60%. 2, 3, 5 The sensitivity is also influenced by other factors, including the timing of the test and the cause of the pancreatitis. The amylase level is also helpful to see how the treatment for ...
TY - JOUR. T1 - Effect of nucleotide polymorphism in cis-regulatory and coding regions on amylase activity and fitness in Drosophila melanogaster. AU - Goto, Hiroki. AU - Szmidt, Alfred. AU - Yamazaki, T.. AU - Inomata, N.. PY - 2005/11/1. Y1 - 2005/11/1. N2 - In natural populations of Drosophila melanogaster, there are many amylase (AMY) isozymes encoded by the duplicated genes, but their adaptive significance remains unclear. One approach to elucidate this issue is to understand the molecular basis of functional differences between the allelic classes. In this study, the effects of nucleotide polymorphism in 5′-flanking (cis-regulatory) and coding regions on AMY activity were examined, both on glucose and starch food media and in larvae and adults, using three chimeric Amylase (Amy) genes, Amyc111, Amyc161 and Amy fc661. In this notation, the first number in the superscript indicates the sequence of the 5′-flnaking region (similar to Amy 1 or Amy6), the second number refers to the coding ...
In todays world, there seems to be a widespread belief that carbohydrates (more commonly referred as carbs) are bad for you. This couldnt be farther from the truth.. Along with proteins and fats, carbohydrates are one of the 3 main macro-nutrients essential to the human body. Amylase is the digestive enzyme responsible for digesting carbohydrates and converting them into glucose and other forms of sugar. In this article, we will be taking a closer look at what exactly amylase is - and why it plays such a major role in your body.. So without further ado, let us begin!. What is amylase?. As we mentioned above, amylase is a digestive enzyme found in the human body. It is more specifically found in the pancreas and the salivary gland where it breaks down sugar and starch and converts them into more simple forms of sugar that can later be used for energy. While amylase is mainly found in humans, it is also found in other animals as well as plants.. Why is amylase important?. Amylase plays a key ...
We have recently summarized the evidence that the amylase of the serum is of heterogenous origin (1). Studies in this laboratory on the regulation of blood amylase levels (2, 3) have shown that [1] normal blood amylase is controlled in part at extrapancreatic sites, [2] blood amylase is lowered in states of increased carbohydrate utilization, and [3] the amylase responses to alterations of carbohydrate metabolism are markedly altered by liver disease but unaffected by total pancreatectomy. These findings led to the conclusion that the liver contributes to the blood amylase level and is an important site of regulation. Similar conclusions ...
Serum amylase and lipase measurements are often used to diagnose acute pancreatitis. This study addresses the question of whether it is advantageous to order serum amylase and lipase tests simultaneously. We evaluated performance of the two tests separately and in combination through a retrospective study of patients for whom both amylase and lipase determinations were ordered. Initial analysis of test performance was conducted with a uniformly applied criterion based on determination of optimal sensitivity-specificity pairs. Individual tests and combinations of tests, including the AND and OR rules and discriminant functions, were examined. Only the discriminant approach demonstrated better performance than the lipase test alone. This finding was subsequently confirmed by logistic regression analysis. We conclude that ordering both tests simultaneously can be advantageous in diagnosing acute pancreatitis when a bivariate approach is used; however, this must be weighed against the ...
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TY - JOUR. T1 - Isolated pancreatic acini from suckling and weanling rats. T2 - Changes in amino acid incorporation and carbachol-stimulated amylase secretion with age. AU - Pollack, Paul F.. AU - Verbridge, Jill. AU - Thornburg, William. AU - Koldovsky, Otakar. AU - Korc, Murray. PY - 1986/1/1. Y1 - 1986/1/1. N2 - To characterize the changes in pancreatic function during postnatal development, isolated pancreatic acini were prepared from rats aged 8-9, 12-14 and 20 days and from adult rats. Isolated acini maintained a normal microscopic appearance and viability as judged by exclusion of trypan blue and linear incorporation of tritiated leucine into total protein. The rate of incorporation in 8-day-old acini was 20% of that observed in adult rats. Significant dose-dependent increases in amylase release in response to carbachol were observed in all age groups; stimulated amylase secretion was significantly less in the 8- to 9- and 12- to 14-day-old animals than in the 20-day-old and adult rats. ...
Scientists published a paper in Nature Genetics Sunday in which they studied an enzyme called amylase thats produced in saliva and breaks down starch. Human amylase genes share a common ancestry with the amylase gene found in our close relative, the chimpanzee. But they are different in some important ways. Instead of one amylase gene, we have several. Human amylase genes range from 2 to 15 copies, averaging three times as many as chimpanzees. But our extra copies not sprinkled randomly across the worlds population. European Americans and tuber-eating African hunter-gatherers known as the Hadza have a lot of copies. Yakuts, who live in Siberia and eat mostly fish, and Mbuti pygmies who eat mostly animals they hunt, have fewer.. See a pattern? The people with few amylase genes are hunters who dont have much starch in their diet. The people with a lot of genes eat a lot of starch. How did this pattern arise? As cells give rise to eggs and sperm, chunks of their DNA sometimes get accidentally ...
Increased variability and abnormalities in pancreatic enzyme concentrations in otherwise asymptomatic subjects with type 2 diabetes Jaret Malloy, Kate Gurney, Kevin Shan, Ping Yan, Steve ChenAmylin Pharmaceuticals LLC, San Diego, CABackground: Recent studies have demonstrated an increased incidence of pancreatitis in patients with type 2 diabetes compared with obese nondiabetic individuals. Serum lipase and pancreatic amylase concentrations are used in conjunction with clinical findings to diagnose pancreatitis.Methods: In two large clinical trials of overweight/obese nondiabetic and type 2 diabetic subjects, lipase and pancreatic amylase were measured at screening and 2–5 weeks later at baseline (prior to treatment with study medication).Results: Lipase and pancreatic amylase concentrations were above the upper limit of normal (ULN) in 13% and 6% of type 2 diabetic subjects, respectively, and were approximately three-fold (3 ×) higher than the proportion of nondiabetic subjects with levels
α-amylases are enzymes which hydrolyze starch molecules to give diverse products including dextrins and progressively smaller polymers composed of glucose units which causes hyperglycemia and development of type 2 diabetes mellitus. Therefore, we estimated the α-amylase activity, HbA1C and blood glucose in Indian type 2 diabetic patients. Study group s follows: 20 Healthy subjects, 30 newly detected patients and 43 type 2 diabetics on treatment with metformin and pioglitazone combination and 30 pioglitazone alone treated. In all subjects blood sugar was estimated and anti diabetic activity criterion was taken for the diagnosis. In the same subjects fasting and post-prandial α-amylase activity was estimated, HbA1c levels were compared with α-amylase activity in healthy and treated subjects. The α-amylase activity in type 2 diabetic patients was significantly different from control subjects and significant relation between α-amylase activity and HbA1c was observed in diabetic subjects ...
α-amylase is a very important enzyme, which is widely used in food processing, food industry, brewing, fermentation, textile industry and pharmaceutical industry, etc. It is one of the most widely used enzymes. As it has wide application, α- amylase has great commercial value.. In this report, ASKCI will further disclose market potential and commercial opportunities for α-amylase industry in China. Recommendations will be given at the end of the report to describe the prospect and provide suggestions for strategy making.. Browse Full Report Visit - http://www.acutemarketreports.com/report/market-analysis-and-prospect-of-alpha-amylase-industry-in-china-2015-2020-proposal. 1)The Aim of Report. -To provide readers with comprehensive and in-depth understanding of Chinese α-amylase industry;. -To analyze the market of the industry;. -To discover drivers and barriers of the industry;. -To reveal opportunities for α-amylase industry in China.. 2)Benefit from the Report. -Obtain latest info of ...
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Computer graphics representation of taka amylase, an enzyme. Amylases are a group of related enzymes that break down starch, glycogen and other polysaccharides. Plants contain both the alpha and beta types of amylases; animals possess only the alpha form, found in pancreatic secretions and (in humans) in saliva. Amylases break the long polysaccharide chains, leaving a mixture of the sugars glucose and maltose. Blue sticks, light blue ribbons. - Stock Image A605/0025
Technically, starch blockers are amylase inhibitors. Amylase is one of the main enzymes the body uses to digest starch. In theory, when amylase is blocked, starch can pass through the body undigested. If the body is unable to digest a part of the meal the calorie intake will be lower which can aid in weight loss. However, theory is one thing, reality another and clinical studies of amylase inhibitors have not established definitive benefits.1-3 For example, one published study failed to find that use of a phaseolus product reduced the usual blood sugar rise that follows a meal.7 Several possible reasons for this discrepancy have been proposed, such as that the amylase inhibitor may be broken down and made ineffective in the stomach, the product may supply enough of its own amylase to counteract any benefit, and that another enzyme, glucoamylase, may be able to take over when amylase cant do the job. However, according to the manufacturer of a current product, more concentrated extracts of ...
Amylases are enzymes that help convert starch into sugars. It is common in mammal saliva where it helps to start digestion. If youve ever chewed a starchy food for long enough for it to become sweet, amylases are responsible for that. Amylases are also instrumental in the process of fermentation.. ...
α-Amylase hydrolyzes the 2-chloro-p-nitrophenyl-α-D-maltotrioside (CNPG3) to release 2-chloro-nitrophenol and form 2-chloro-p-nitrophenyl-α-D-maltoside (CNPG2), maltotriose (G3) and glucose (G). The rate of increase in absorbance is measured at 405 nm and is proportional to the α-amylase activity in the sample.(G). The rate of increase in absorbance is measured at 405 nm and is proportional to the α-amylase activity in the sample.. ...
Introduction & Objective: Alpha amylase is the most important decomposing enzyme in starch. Digestion and absorption of starch in the intestine can be prevented and also the blood sugar levels can be controlled by restrain and control of alpha amylase. The aim of this study was to evaluate the effect ...
Introduction. The Effect that Temperature Has on the Rate at which the Enzyme (Amylase) can Breakdown its Substrate Aim: The aim of this experiment is to find out the effect that the temperature has on the rate at which the enzyme (Amylase) can breakdown its substrate, which is Starch. Amylase: Enzyme having physiological, commercial, and historical significance, also called diastase. It is found in both plants and animals. Amylase hydrolyzes starch, glycogen, and dextrin to form in all three instances glucose, maltose, and the limit-dextrins. Prediction: I predict that as the temperature increases, the speed of the reaction will increase. Since temperature is a measure of the motion of particles, increasing the temperature will cause the particles to move faster. When particles move faster, more collisions occur and the collisions are more violent. This should increase the reaction rate. This is backed up by the collision theory, according to this theory; reacting molecules must collide with ...
Amylase er en gruppe enzymer som spalter stivelse (polysakkarider) til sukker (disakkarider). Amylase finnes i spytt og i sekresjoner fra bukspyttkjertelen. Den starter virkningen på stivelse alt i munnhulen. Stivelse består egentlig av de to polysakkaridene amylose og amylopektin (begge er såkalte K3a karbohydrater ). Amylase spalter amylosen, som er lange sukkerkjeder, til to og to glukoseenheter (disakkarider) av gangen, slik at det kan dannes maltose. På samme måte spalter amylasen amylopektin, men prosessen stopper opp når den når fram til forgreningene (1-6 bindingene) og danner nye svært forgrenede molekyler som er den del av polysakkaridet dekstrin. Dette spaltes av et annet enzym (isomaltase) til maltose (et disakkarid). Et forhøyet nivå av amylase i blod og urin kan være et signal på betennelse i bukspyttkjertelen, såkalt pancreatitt. ...
α-Amylase is a protein enzyme EC 3.2.1.1 that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. It is the major form of amylase found in humans and other mammals. It is also present in seeds containing starch as a food reserve, and is secreted by many fungi. Although found in many tissues, amylase is most prominent in pancreatic juice and saliva, each of which has its own isoform of human α-amylase. They behave differently on isoelectric focusing, and can also be separated in testing by using specific monoclonal antibodies. In humans, all amylase isoforms link to chromosome 1p21 (see AMY1A). Amylase is found in saliva and breaks starch into maltose and dextrin. This form of amylase is also called ptyalin /ˈtaɪəlɪn/ It will break large, insoluble starch molecules into soluble starches (amylodextrin, erythrodextrin, and achrodextrin) producing successively smaller starches and ultimately maltose. Ptyalin acts on linear ...
Amylase crystals are a herblore secondary used in the creation of stamina potions. A player can mix amylase crystals with a Super energy potion at 77 Herblore to make a stamina potion. One amylase crystal creates a single dose of stamina potion from a Super energy(1), and as such, four amylase...
Fran, We use the monoclonal anti- Pancreatic Amylase Cat# MU099-UC from Biogenex. Formaldehyde-fixed(16-24hrs) paraffin-embedded, 4 micron thick sections are dried overnight at 45C, de-waxed in Xylene 3X10 min., rinsed X2 in 100% ETOH, endogenous peroxidase blocked in 5% H2O2 in methanol for 30 min., 95% ETOH X2 @ 1min, 70%ETOH-water. Sections are pretreated by digestion for 10 [email protected] in 0.6% w/v Ficin dissolved in 0.01M NaOH containing 0.1mg CaCl2 per 100ml, pH is adjusted to pH7.8 with 1/N HCl. Following blocking in 1% v/v Normal Goat Serum (NGS) in TBS, sections are incubated for 1 hour at RT in 1:100 dilution of Ab in 1%NGS in TBS. Detection is with LSAB(Zymeds Histostain, GaM), chromogen is AEC. The results have always been consistent on our controls( pancreas fixed in NBF for several time points), test specimens are prone to reflect the quality of fixation and processing( particularly short fixation!). Hope this helps Bryan , ---------- , From: [email protected][SMTP:[email protected]] , ...
In this experiment, students investigate and compare amylase activity in different life stages of barley. The experiment allows students to make connections between concepts and processes previously covered in lectures and reading materials and is relevant to learning and skills development in the scientific method and related areas such report writing and the interpretation of results. With regard to theoretical and conceptual knowledge, the practical investigates the function of an enzyme in a living organism and explores concepts around the ontogenic regulation of metabolism in an organism as it develops. Development of skills in the scientific method and the communication of science are also important components of this exercise.. ...
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A 2013 study published in the Journal of Clinical & Diagnostic Research was designed to determine the serum amylase, blood glucose and the serum lipid profile in 110 type 2 diabetes patients compared to healthy individuals of the same age and sex. The research showed that for the diabetic subjects wherever blood sugar levels were higher, serum amylase activity was found to be significantly lower. This finding was reflective of pancreas malfunction and speaks to the importance of a healthy pancreas producing healthy amounts of amylase. ...
Amylase in the blood is important for undisturbed performance of all body functions. Check the level of Amylase in the body and avoid disease.
Amylase is a an enzyme that is somewhat unique in that it is produced at two points in your body. It first activates as a component of saliva, where it begins the breakdown of starches into smaller, more easily digested components. Further on in the digestive process, it is created again in the pancreas. At this point, it is referred to as pancreatic amylase, and it works to
Bacillus licheniformis RM44 was isolated from hot spring near Karachi and screened forthe production of extracellular amylase Amy RM44. Amy RM44 was purified to homogeneityon a single step by affinity chromatography using insoluble corn starch. The molecular weightof Amy RM44 was estimated to be 66 kDa by SDS-PAGE and zymographic analysis. Nine foldpurification was achieved with the specific activity of 870 U/mg that provides the total yieldof the enzyme up to 31%. Studies on purified AmyRM44 characterization revealed that theoptimum temperature of enzyme was 100 ºC. Amy RM44 was proved to be highly thermostableas it retained 50% activity after 2 h at 100 ºC. Amy RM44 was stable over wide range of pHwith optimum activity at pH 5. Enzyme activity was not significantly inhibited by SDS andEDTA. Amy RM44 also exhibited its activity towards various carbohydrates such as dextrin,pullulan, α-cyclodextrin, β-cyclodextrin, and γ-cyclodextrin.
The optimum pH of salivary amylase is approximately 6.7 to 7, according to Worthington Biochemical Corporation, meaning very slightly acidic. If the pH is significantly out of this range, the enzyme...
Essential Therapeutics Digestive Enzymes is a high potency pancreatic supplement that breaks down fats, proteins, and carbohydrates to prevent malabsorption and gastric discomfort.
Lists the various brand names available for medicines containing amylase. Find information on amylase use, treatment, drug class and molecular formula.
An amylase test measures the amount of this enzyme in a sample of blood taken from a vein or in a sample of urine. Many conditions can change amylase levels. Your doctor will discuss any significant abnormal results with you in relation to your symptoms and past health.. Results are normally available within 72 hours. The normal values listed here-called a reference range-are just a guide. These ranges vary from lab to lab, and your lab may have a different range for whats normal. Your lab report should contain the range your lab uses. Also, your doctor will evaluate your results based on your health and other factors. This means that a value that falls outside the normal values listed here may still be normal for you or your lab.. ...
Hellenic Society of Gastroenterology. Annals of Gastroenterology.Journal part.Scientific article.Article.2007 . Creators: Kokozidis G, Kinigopoulou P., Kapetanos D., Paikos D., Papaioannou A., Progia E., Kitis G., ..Pancreatitis is the most common of the serious complications of ERCP, with rates between 2 and 40%. The aim of this study was to evaluate the significance of serum levels of amylase, lipase and elastase after ERCP, in the prediction and diagnosis of pancreatitis. Patients: Forty three consecutive patients underwent ERCP for various indications. Methods: Serum samples were taken before, one hour and six hours after the procedure. Amylase was evaluated with enzymatic colourimetric test (Olympus, normal2N) the first hour and 26 (60%) the sixth. Twenty seven (62%) had serum lipase elevation (|2N) the first hour and 30 (70%) the sixth. Four (9%) had increased elastase (|2N) the first hour and 2 (4%) the sixth. The only enzyme that was significantly elevated in the patients who developed
In Diabetic ketoacidosis when pain abdomen is one of the major clinical finding, serum amylase levels are obtained, to rule out the diagnosis of acute pancreatitis (since the latter too can present with acute pain abdomen). If the serum amylase levels are found high in patients with Diabetic ketoacidosis, it may not be due…
Background. Serum lipase and amylase are biochemical analyses used to establish the diagnosis of acute pancreatitis (AP). Despite lipase having been shown internationally to be a more sensitive and specific test, amylase remains a popular first-line test. Objective. To provide a local basis for the recommendation of the best first-line laboratory test, an assessment of their performance in our local setting was undertaken. Methods. From a prospective dataset on patients with acute abdominal pain and raised serum lipase and/or amylase values, the sensitivity and specificity of serum lipase, amylase and the two in combination was calculated for the diagnosis of AP, as defined by the Atlanta criteria. Results. During the study period, 476 patients presented with acute upper or generalised abdominal pain and raised serum amylase and/or lipase values. The median age of the patients was 43 years (range 14 - 85), and 58% were men and 42% women. Of the patients, 322 (68%) presented with abdominal conditions
Allcosmeticsource.com Fungal amylase 1000000u/g,5kg/bag,free shipping [EP170508011]- Fungal amylase 1000000u/g,5kg/bag,free shipping What is Fungal amylase 1000000u/g? Fungal amylase, also known as fungal alpha amylase (1, 4- alpha -D- glucan hydrolase), is refined by Aspergillus oryzae Wahl deep culture, extraction and other processes. The enzyme is endo amylase, which can rapidly hydrolyze the starch, amylose and amylopectin aqueous solution of alpha -1,
To the Editors: Hyperamylasemia has been described in association with numerous solid malignant tumors (1, 2), but its association with hematologic malignancies has not been extensively studied. In a preliminary study, we found that 32% of patients with hematologic malignancies had hyperamylasemia (2). To gain more information regarding the incidence and significance of hyperamylasemia in patients with hematologic malignancies, we studied serum amylase activity in 35 patients and followed them over time (3, 4).. Forty-one consecutive patients with various hematologic malignancies were evaluated in our hematology clinic. Six patients were excluded because of concurrent illness or misdiagnosis of a hematologic ...
A CCK-deficient mouse model was successfully generated by gene targeting in ES cells. The RIA data presented here confirm that this new mutant mouse strain does not produce CCK. CCK-deficient mice are viable and grow at a normal rate. Aging mice (1 yr and older) continued to thrive, with no obviously abnormal pathologies. The body weight, rate of weight gain (Fig. 1 B), and total daily food intake (data not shown) of CCK-deficient mice do not differ from wild-type mice, suggesting that CCK is not essential for maintaining normal energy balance (intake vs. expenditure).. Analysis of pancreatic enzyme content in CCK-deficient mice fed the basal diet revealed an increase in amylase enzyme activity (Table 3). Because chymotrypsinogen content remained unaltered in the mutant, we concluded that the increase in amylase is not due to a general reduction in enzyme secretion but a specific increase in amylase synthesis. Previous studies have demonstrated an inhibitory effect of CCK on amylase synthesis ...
Background and Aims: This study examined the stress levels, the stressors and biomarker such as Alpha (α)-Amylase enzyme which is secreted under distress conditions. The aim was to determine the relationship between these three variables. Methods: The study respondents were made up of cancer outpatients from 3 hospitals namely the Hospital Kuala Lumpur, Seberang Jaya and Johor Baru, Pantai Ipoh and Putrajaya. The Personal Stress Inventory (PSI) questionnaire was used to identify stressors, while the Hospital Anxiety and Depression Scale (HADS) were used to determine the psychological distress levels. The Salimentary Oral Swab (SOS) Technique was used to collect the saliva and then the Salivary α-Amylase Assay Kit was used to analyse for α amylase. Results: The majority of respondents were stressed due to their sensitive emotion and nervousness in their daily lives (68.7%), they had poor memory and short attention spans of carrying out job tasks (67.3%) as well as they were emotionally
Four mulberry silkworm races viz., Pure Mysore, Nistari, NB4D2 & CSR2 and two hybrid (Pure Mysore × CSR2 and Nistari × NB4D2 ) silkworms were selected for the present study. The specific activity of amylase in the haemolymph as well as midgut tissue was estimated. The qualitative analysis of amylase was carried out by Native-PAGE. The commercial characters viz., fecundity, larval weight, larval duration, cocoon weight, shell weight, shell ratio, filament length, denier and renditta were selected. The results of quantitative analysis were subjected for statistical analysis against selected commercial characters to know the level and kind of correlation between them. The results of statistical analysis clearly showed that haemolymph amylase has highly positive correlation with selected commercial characters except larval duration. In contrast, the midgut amylase indicated highly positive correlation with larval duration only. The zymograms of amylase also exhibited variation among the selected ...
Objective To investigate the effects of microbial enteral nutrition on serum amylase,hs-CRP and gastrointestinal function in children with acute pancreatitis at different stages. Methods A total of 120 children with acute pancreatitis who received treatment in our hospital from May 2012 to June 2016 were selected as the subjects of the study. All the children were randomly divided into the super-early group and the early group. The super-early group was treated with microbial enteral nutrition support after 24 hours of admission.In the early group,the microbial enteral nutrition support intervention was performed at 72 hours after admission. After treatment,the serum amylase,hs-CRP and gastrointestinal function were compared and analyzed. Results The rate of good nutrition tolerance in the early group was significantly lower than that in the super-early group( P 0. 05). There was no significant difference between the two groups in the mortality group. The length of hospital stay was 36. 94 ± 7. 19 d
Camelids produce functional antibodies devoid of light chains and CH1 domains. The antigen-binding fragment of such heavy chain antibodies is therefore comprised in one single domain, the camelid heavy chain antibody VH (VHH). Here we report on the structures of three dromedary VHH domains in complex with porcine pancreatic alpha-amylase. Two VHHs bound outside the catalytic site and did not inhibit or inhibited only partially the amylase activity. The third one, AMD9, interacted with the active site crevice and was a strong amylase inhibitor (K(i) = 10 nm). In contrast with complexes of other proteinaceous amylase inhibitors, amylase kept its native structure. The water-accessible surface areas of VHHs covered by amylase ranged between 850 and 1150 A(2), values similar to or even larger than those observed in the complexes between proteins and classical antibodies. These values could certainly be reached because a surprisingly high extent of framework residues are involved in the interactions ...
The pancreas and the glands that produce saliva also produce amylase, an enzyme that helps the body digest food, notes MedlinePlus. Amylase enters the bloodstream when the pancreas becomes inflamed...
Semin Gastrointest Dis ; Bacterial contamination of pancreatic necrosis: Is CT guided fine needle aspiration helpful in patients with infected necrosis. Acute pancreatitis dietary management MD, Mittal A, van den Heever M, et al, for the Pancreas Network of New Zealand. MRI may also identify early duct disruption that Acute pancreatitis dietary management not seen on CT. The serum amylase level is IU per liter, the serum lipase level is IU per liter, the serum alanine aminotransferase level is Acute pancreatitis dietary management per liter, and the serum lactate dehydrogenase level Acute pancreatitis dietary management IU per liter. For patients with mild acute pancreatitis, nasojejunal feedings can be avoided unless patients are unable to tolerate oral intake for over 1 week. Most of these collections resolve Acute pancreatitis dietary management. Evaluation of severity in patients with acute pancreatitis. Radiol Clin North Am. Generally, however, pancreatic stents are difficult to monitor, ...
A maltogenic amylase (MAG1) from alkaliphilic Bacillus lehensis G1 was cloned, expressed in Escherichia coli, purified and characterised for its hydrolysis and transglycosylation properties. The enzyme exhibited high stability at pH values from 7.0 to 10.0. The hydrolysis of β-cyclodextrin (β-CD) produced malto-oligosaccharides of various lengths. In addition to hydrolysis, MAG1 also demonstrated transglycosylation activity for the synthesis of longer malto-oligosaccharides. The thermodynamic equilibrium of the multiple reactions was shifted towards synthesis when the reaction conditions were optimised and the water activity was suppressed, which resulted in a yield of 38% transglycosylation products consisting of malto-oligosaccharides of various lengths. Thin layer chromatography and high-performance liquid chromatography analyses revealed the presence of malto-oligosaccharides with a higher degree of polymerisation than maltoheptaose, which has never been reported for other maltogenic amylases. The
Starch is an inexpensive and renewable raw material for numerous industrial applications. However, most starch-based products are not cost-efficient due to high-energy input needed in traditional enzymatic starch conversion processes. Therefore, α-amylase with high efficiency to directly hydrolyze high concentration raw starches at a relatively lower temperature will have a profound impact on the efficient application of starch. A novel raw starch digesting α-amylase (named AmyZ1) was screened and cloned from a deep-sea bacterium Pontibacillus sp. ZY. Phylogenetic analysis showed that AmyZ1 was a member of subfamily 5 of glycoside hydrolase family 13. When expressed in Escherichia coli, the recombinant AmyZ1 showed high activity at pH 6.0-7.5 and 25-50 °C. Its optimal pH and temperature were 7.0 and 35 °C, respectively. Similar to most α-amylases, AmyZ1 activity was enhanced (2.4-fold) by 1.0 mM Ca2+. Its half-life time at 35 °C was also extended from about 10 min to 100 min. In comparison, AmyZ1
TY - JOUR. T1 - Unexpected mode of action of sweet potato β-amylase on maltooligomer substrates. AU - Fazekas, Erika. AU - Szabó, Katalin. AU - Kandra, L.. AU - Gyémánt, G.. PY - 2013. Y1 - 2013. N2 - β-Amylase (EC 3.2.1.2), one of the main protein of the sweet potato, is an exo-working enzyme catalyzing the hydrolysis of α(1,4) glycosidic linkages in polysaccharides and removes successively maltose units from the non-reducing ends. The enzyme belongs to glycoside hydrolase GH14 family and inverts the anomeric configuration of the hydrolysis product. Multiple attack or processivity is an important property of polymer active enzymes and there is still limited information about the processivity of carbohydrate active enzymes. Action pattern and kinetic measurements of sweet potato β-amylase were made on a series of aromatic chromophor group-containing substrates (degree of polymerization DP 3-13) using HPLC method. Measured catalytic efficiencies increased with increasing DP of the ...
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Name of Enzyme Substrate Location Task Products Salivary Amylase (likes pH level of around 6.2-7.4) Amylose in the mouth Saliva which is released from the salivary glands Aid in the hydrolysis of the starch amylose into the disaccharide maltose (glycocidic bond) Maltose, and Amylose Pepsinogen No substrate, inactive form of pepsin Produced in the gastric… ...
Oral health is related to general health and one of the most prevalent chronic diseases is diabetes mellitus. Diabetes can have adverse effects on oral health and vice versa. Saliva analysis can be used as a non-invasive method to obtain information about diseases status like diabetes. The aim of present study was to evaluate the salivary immunoglobulin-A (s-IgA) and salivary amylase levels and their associations with oral-dental manifestations in patients with controlled and non-controlled type 2 diabetes. This case-control study was carried out on 90 subjects who referred to the Diabetes Center of Shahid Bahonar Hospital, Kerman University of Medical Sciences, Kerman, Iran. Participants were divided into three groups: 1) uncontrolled diabetic patients (n = 30); 2) controlled diabetic patients (n = 30); and 3) healthy individuals (n = 30). Unstimulated salivary levels of I-A and amylase were measured. All participants underwent a dental and periodontal examination to explore the oral and dental
TY - CHAP. T1 - Elevation of pancreatic enzymes in bile of PBM. AU - Fujimoto, Takaaki. AU - Ohtsuka, Takao. AU - Nakamura, Masafumi. PY - 2018/6/5. Y1 - 2018/6/5. N2 - Pancreaticobiliary maljunction (PBM) is a congenital anomaly in which the reciprocal reflux between pancreatic and bile juice occurs because the conflu- ence of the pancreatic and bile ducts lies outside of the area of contractile influence of the sphincter of Oddi. The reflux of pancreatic juice into the bile duct and subse- quent elevated pancreatic enzyme level in bile are usually recognized in PBM. Amylase is the most popular pancreatic enzyme for assessment in daily prac- tice, and assessment of amylase level in bile is useful to definitively confirm the presence of PBM, and the mixture of pancreatic and bile juices may produce various physiological and pathological alterations. In PBM, the amylase level in bile in patients with biliary cancers has been reported to be higher than that with benign biliary diseases; however, ...
Phytic acid has a strong binding affinity to minerals such as calcium, magnesium, iron, copper, and zinc. This results in precipitation, making the minerals unavailable for absorption in the intestines.[2][3] Phytic acids are common in the hulls of nuts, seeds and grains and of great importance in agriculture animal nutrition and eutrophication wise due to the mineral chelation and bound phosphates released in to the environment. Without the need to use milling to reduce phytate (including nutrient),[4] the amount of phytic acid is commonly reduced in animal feeds by adding histidine acid phosphate type of phytases to them.[5] Protease inhibitors are substances that inhibit the actions of trypsin, pepsin and other proteases in the gut, preventing the digestion and subsequent absorption of protein. For example, Bowman-Birk trypsin inhibitor is found in soybeans.[6] Lipase inhibitors interfere with enzymes, such as human pancreatic lipase, that catalyze the hydrolysis of some lipids, including ...
Research question What is the effect of temperature Amylase activity? Word count-1453 Background research Enzymes are biological catalysts that speed up a...
I have done a native PAGE and activity staining for amylase which consists of initial incubation with 1% starch for 30mins followed by 2% potassium iodide and 0.2% iodine. the activity bands appear translucent on a blue background. but when i cut the activity bands it always appears blue. now i wish to process these cut gel bands for MALDI TOF for which i have to destain. please can anyone help me with destaining process?????. ...
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2. Activation of ERK pathway in Pancreatic Acinar Cells (Figure 1). ERK activation is usually monitored by following the dual phosphorylation of the Thr and Tyr residues in the Thr-Glu-Tyr activation sequence brought about by MEK as there are a number of good phosphospecific antibodies directed at this epitope. It can also be shown by phosphorylation of myelin basic protein either in a test tube or by an in gel technique following gel electrophoresis and renaturization. Both Western blots and the in gel kinase procedure reveal the two forms of ERK at approximately 44 and 42 kDa; in fact, the molecules were originally referred to as p42 and p44 MAPK with p42 being what is now referred to as ERK2 and p44 now being ERK1. Using isolated rat or mouse pancreatic acini in vitro, ERK1/2 is activated by CCK, bombesin, substance P, and carbachol, all of which activate G protein coupled receptors coupled to Gq and calcium mobilization but not by secretin or VIP which activate receptors coupled to Gs and ...
Introduction: Amylase is an enzyme found in human saliva. This enzyme can digest starch molecules and break them down to sugar molecules. (A starch molecule is a polymer of sugar molecules). Learning about the role of enzymes in digestion can help us to understand the body metabolism and the way that foods digest and become usable for the body. ...
2c and d), this observation is proof of the existence not only of a commensalism, but a synergism between B. amyloliquefaciens and S. cerevisiae. Synergism is regarded as the ability of two or more organisms to bring about changes (usually chemical) that neither can accomplish alone [16]. The same kind of synergism may also exist between L. fermentum 04BBA15 and S. cerevisiae, since there was a rise of α-amylase production when the two strains were cultivated together. Synergism in both cases could be explained by the fact. that in starch broth B. amyloliquefaciens 04BBA15 and L. fermentum 04BBA19 hydrolyze starch which leads to the increase in glucose or other oligosaccharids that the yeast S. cerevisiae needs for a normal growth since it is unable to convert starch into glucose. Part Epigenetic inhibitor manufacturer of the glucose U0126 supplier release through starch hydrolysis is immediately utilized by S. cerevisiae. The increase in α-amylase production could be attributed to the rapid ...
A total of 36 studies out of 17,845 were identified and included. Of these 36 studies, only 5 were clinical trials involving 580 patients, and the remaining 31 were animal studies with 1623 rats or mice. 24 studies focused on the treatment of AP with NSAIDs and 12 on AP-associated systematic complications. Both preclinical and clinical studies showed that NSAIDs may have beneficial effects against AP-related injuries. 9 of the 14 preclinical studies stated that NSAIDs reduced the serum amylase level significantly, and 6 of 7 showed that NSAIDs lowered the lipase level markedly. 17 experimental studies all demonstrated that NSAIDs reduced the inflammation. Histopathological examinations indicated that NSAIDs significantly improved the histopathological damages. Similarly, clinical evidence showed that NSAIDs are effective in suppressing proinflammatory cytokines, relieving pain, ameliorating systematic complications and reducing mortality. In the included 5 clinical studies, serious adverse ...
A total of 36 studies out of 17,845 were identified and included. Of these 36 studies, only 5 were clinical trials involving 580 patients, and the remaining 31 were animal studies with 1623 rats or mice. 24 studies focused on the treatment of AP with NSAIDs and 12 on AP-associated systematic complications. Both preclinical and clinical studies showed that NSAIDs may have beneficial effects against AP-related injuries. 9 of the 14 preclinical studies stated that NSAIDs reduced the serum amylase level significantly, and 6 of 7 showed that NSAIDs lowered the lipase level markedly. 17 experimental studies all demonstrated that NSAIDs reduced the inflammation. Histopathological examinations indicated that NSAIDs significantly improved the histopathological damages. Similarly, clinical evidence showed that NSAIDs are effective in suppressing proinflammatory cytokines, relieving pain, ameliorating systematic complications and reducing mortality. In the included 5 clinical studies, serious adverse ...
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In the pig parotid gland, ATPDase was mainly associated with duct epithelium, with very little, if any, immunoreactivity on acinar cells. Myoepithelial cells dispersed among acini produced a significant signal. There have been reports of Ca2+-dependent ATPases associated with isolated parotid acinar cells, but as judged by their biochemical properties, these ATPases are different from ATPDases. However, a nucleoside triphosphatase described by Sato et al. (29) in bovine parotid gland and an apyrase described by Valenzuela et al. (34) in a microsomal fraction of rat salivary gland appear to correspond to the ATPDase described in this study and hence could well be associated with ductal epithelial and myoepithelial cells. In agreement with these findings, some studies on rat parotid acini show very little ecto-ATPase activity (9). Some P2Zpurinoceptors have been described on rat parotid acinar cells, which respond to ATP and mediate a Ca2+increase caused by both an influx and a mobilization from ...
1. Enzymes are complex proteins produced by living cells that catalyze specific biochemical reactions usually at body temperature (98.6 F or 37 C). Enzymes are usually sold in powder form and are obtained from animal, plant (particularly fungal), and bacteriological sources. In addition to their source designation, enzymes are also described in terms of unit definition, activity, and purity.. 2. The α-amylases attack randomly along the amylose chain (the major component of wheat starch) to convert it into dextrins (short-chained polysaccharides), which are very water soluble. The β-amylases attack amylose only from one end at a time and are therefore much less effective compared to α-amylases. Diastases contain both α- and β-amylases and, therefore, are not as effective as the α-amylases alone. Proteases cleave the bonds of proteins leading to shorter chain polypeptides and finally to amino acid molecules, which are water soluble. Trypsins, which come from the ferments of pancreatic ...
Results Absence and reduced intracellular levels of 5-HT inhibited the secretion of zymogen granules both ex vivo and in vitro and altered cytoskeleton dynamics. In addition, absence of 5-HT resulted in attenuated pro-inflammatory response after induction of pancreatitis. TPH1−/− mice showed limited zymogen release, reduced expression of the pro-inflammatory chemokine MCP-1 and minimal leucocyte infiltration compared with wild-type animals. Restoration of 5-HT levels in TPH1−/− mice recovered the blunted inflammatory processes observed during acute pancreatitis. However, cellular damage, inflammatory and fibrotic processes accelerated in TPH1−/− mice during disease progression.. ...
Pancreatic amylase is not produced by your little one (in sufficient quantities) until they are a year old - sometimes even later. The rule of thumb here is that it is not until your little ones molar teeth are fully developed that they have sufficient quantities of pancreatic amylase to properly digest grains - this can be anywhere from 13 - 24 months of age.
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A study, published in the Internal Journal of Medical Science, conducted a double-blind, placebo-controlled study, in which 60 slightly overweight people were given either placebo or Phase 2, a highly concentrated phaseolus extract, once daily.. This single daily dose was given 30 minutes prior to a large meal rich in carbohydrates. Over the 30 days of the study, subjects receiving Phaseolus vulgaris extract had significantly greater reduction of body weight, BMI, fat mass, adipose tissue thickness, and waist/hip/ thigh circumferences while maintaining lean body mass compared to subjects receiving placebo.. research notes: Layer, P., et al. Effect of purified amylase inhibitor on carbohydrate tolerance in normal subjects and patients with diabetes mellitus. MAYO Clinic Proceedings 61, 6 (June, 1986) 442-447.. Brugge, W.R. and M. S. Rosenfeld. Impairment of starch absorption by a potent amylase inhibitor. American Journal of Gastroenterology 82, 8 (August, 1987) 718-722.. Layer, P., et al. ...
contains a pure and clean source of New Zealand bovine pancreas glandular which is loaded with enzymes, B-vitamins, and minerals designed to support normal blood sugar levels and healthy pancreatic function
Check your inbox for an e-mail with a link to download the recipes Digestive enzymes aid in the absorption of nutrients and assist in the breaking down fwt the food particles you eat. This process allows the food that you eat to be used as energy, to support the building of new muscle and nerve cells, as well as protecting your blood from toxins.. Inadequate enzyme production can lead to digestive discomfort, gas, bloating, low energy and allergy-like emzymes to food. The three main categories of digestive enzymes are amylase, protease and lipase. Amylase in needed to break down carbohydrates, dodn whole grains, white flours, sugars and starchy vegetables. Amylase is found in pancreatic and intestinal juices, but is also found in your saliva.. This means that the digestive process of carbohydrates actually begins in the mouth, making chewing your food really well even more important. Protease helps you to digest protein. The proper digestion of protein is vital. Undigested protein particles can ...
DiseaseFix brings interesting disease graphics, videos, pictures, photos, and images on Acute Pancreatitis. Watch graphics along with explanatory content at one place for Acute Pancreatitis. Causes, Symptoms, Diagnosis, Treatment.
This test measures the level of the enzyme amylase in the blood serum. This enzyme is specific to the pancreas and raised levels suggest damage to the pancreas as in acute and chronic pancreatitis. I would usually do this test along with liver function tests. Another useful test for the pancreas is faecal elastase. Pancreatitis can be caused by excessive alcohol, gall stones, cancer or antioxidant deficiency. Another useful test to diagnose pancreatitis is stool elastase levels. ...
We found that copy number of the salivary amylase gene (AMY1) is correlated positively with salivary amylase protein level and that individuals from populations with high-starch diets have, on average, more AMY1 copies than those with traditionally low-starch diets. Comparisons with other loci in a subset of these populations suggest that the extent of AMY1 copy number differentiation is highly unusual. This example of positive selection on a copy number-variable gene is, to our knowledge, one of the first discovered in the human genome. Higher AMY1 copy numbers and protein levels probably improve the digestion of starchy foods and may buffer against the fitness-reducing effects of intestinal disease ...
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Amylase trypsin inhibitors (ATI) can be found in all gluten containing cereals and are, therefore, ingredient of basic foods like bread or pasta. In the gut ATI can mediate innate immunity via activation of the Toll-like receptor 4 (TLR4) on immune cells residing in the lamina propria, promoting intestinal, as well as extra-intestinal, inflammation. Inflammatory conditions can induce formation of peroxynitrite (ONOO−) and, thereby, endogenous protein nitration in the body. Moreover, air pollutants like ozone (O3) and nitrogen dioxide (NO2) can cause exogenous protein nitration in the environment. Both reaction pathways may lead to the nitration of ATI. To investigate if and how nitration modulates the immunostimulatory properties of ATI, they were chemically modified by three different methods simulating endogenous and exogenous protein nitration and tested in vitro. Here we show that ATI nitration was achieved by all three methods and lead to increased immune reactions. We found that ATI nitrated by
Zenpep with NDC 0023-6114 is a a human prescription drug product labeled by Allergan, Inc.. The generic name of Zenpep is pancrelipase lipase, pancrelipase protease, pancrelipase amylase.
Manufacturer of Alcohol Enzymes - Alpha Amylase Enzyme, Glucoamylase, Neutral Protease Enzyme and Starch Liquefaction Enzyme offered by Aum Enzymes, Borsad, Gujarat.
The present study investigated the potential of thermophilic bacteria isolated from hot springs to simultaneously produce protease and amylase enzymes. Amo
PER VRETBLAD; Biospecific Affinity Chromatography of Sweet-Potato β-Amylase. Biochem Soc Trans 1 December 1974; 2 (6): 1327-1328. doi: https://doi.org/10.1042/bst0021327. Download citation file:. ...
Amylase increased was found in Emergency Central. Emergency Central is a collection of disease, drug, and test information including 5-Minute Emergency Medicine Consult, Daviss Drug, McGraw-Hill Medicals Diagnosaurus®, Pocket Guide to Diagnostic Tests, and MEDLINE Journals created for emergency medicine professionals.
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Amylase[edit]. A high amylase level (twice the serum level or the absolute value is greater than 160 Somogy units) in the ...
α-Amylase[edit]. Main article: Alpha-amylase. The α-amylases (EC 3.2.1.1 ) (CAS 9014-71-5) (alternative names: 1,4-α-D-glucan ... The optimum pH for β-amylase is 4.0-5.0[4] γ-Amylase[edit]. γ-Amylase (EC 3.2.1.3 ) (alternative names: Glucan 1,4-a- ... β-Amylase[edit]. Another form of amylase, β-amylase (EC 3.2.1.2 ) (alternative names: 1,4-α-D-glucan maltohydrolase; ... Both α-amylase and β-amylase are present in seeds; β-amylase is present in an inactive form prior to germination, whereas α- ...
Sherman, H. C., & Thomas, A. W. (1915). Studies on amylases. VIII. The influence of certain acids and salts upon the activity ... Thomas, A. W. (1917). A noteworthy effect of bromides upon the action of malt amylase. Journal of the American Chemical Society ... Isoelectric point of malt amylase. Journal of the American Chemical Society, 46, 1711-6. Thomas, A. W., & Kelly, M. W. (1925). ... Influence of hydrogen-ion concentration upon enzymatic activity of three typical amylases. Journal of the American Chemical ...
Other names in common use include amylase III, and 1,4-alpha-glucan:1,4-alpha-glucan 4-alpha-glucosyltransferase. Nebinger P ( ... Characterization of amylases". Biological Chemistry Hoppe-Seyler. 367 (3): 169-76. doi:10.1515/bchm3.1986.367.1.169. PMID ... Nebinger P (March 1986). "Separation and characterization of four different amylases of Entamoeba histolytica. II. ... March 1986). "Separation and characterization of four different amylases of Entamoeba histolytica. I. Purification and ...
At ASCO 2007, results from the SHARP trial[13] were presented, which showed efficacy of sorafenib in hepatocellular carcinoma. The primary endpoint was median overall survival, which showed a 44% improvement in patients who received sorafenib compared to placebo (hazard ratio 0.69; 95% CI, 0.55 to 0.87; p=0.0001). Both median survival and time to progression showed 3-month improvements; however, there was no significant difference in median time to symptomatic progression (p=0.77). There was no difference in quality of life measures, possibly attributable to toxicity of sorafenib or symptoms related to underlying progression of liver disease. Of note, this trial only included patients with Child-Pugh Class A (i.e. mildest) cirrhosis.[13] Because of this trial Sorafenib obtained FDA approval for the treatment of advanced hepatocellular carcinoma in November 2007.[4] In a randomized, double-blind, phase II trial combining sorafenib with doxorubicin, the median time to progression was not ...
The different classes of amylases are α-amylases, β-amylases, and glucoamylases. The α-amylases hydrolyze starch by randomly ... Amylase breaks down carbohydrates into mono- and disaccharides, so a starch agar must be used for this assay. Once the bacteria ... In humans, amylases are secreted by the pancreas and salivary glands, with both sources of the enzyme required for complete ... Since iodine binds to starch but not its digested by-products, a clear area will appear where the amylase reaction has occurred ...
"Amylase Test". Lab Tests Online. Archived from the original on 29 March 2009. Retrieved 30 October 2020. "Changes in the ... In cases of pancreatitis, there may be elevated levels of lipase or amylase, an enzyme found in saliva and the pancreas. Mumps ... Skrha J, Stepan J, Sixtova E (October 1979). "Amylase isoenzymes in mumps". Eur J Pediatr. 132 (2): 99-105. doi:10.1007/ ...
"Reference range (amylase)". GPnotebook. Ageno W, Finazzi S, Steidl L, et al. (2002). "Plasma measurement of D-dimer levels for ...
The -ase suffix is a libfix derived from "diastase". Amylase DNA polymerase. ...
Salivary amylase: Carbohydrate digestion also initiates in the mouth. Amylase, produced by the salivary glands, breaks complex ... Pancreatic amylase that breaks down starch and glycogen which are alpha-linked glucose polymers. Humans lack the cellulases to ... Amylases split carbohydrates such as starch and sugars into simple sugars such as glucose. ...
"Plant α-amylase inhibitors and their interaction with insect α-amylases: Structure, function and potential for crop protection ... Some plant defensins have also been identified as enzyme inhibitors of α-amylase or trypsin. It is believed that these are ... Richardson M, Bloch Jr C (1991). "A new family of small (5 kDa) protein inhibitors of insect alpha-amylases from seeds or ... "Novel insights on the mechanism of action of α-amylase inhibitors from the plant defensin family". Proteins: Structure, ...
Goesaert, Hans; Slade, Louise; Levine, Harry; Delcour, Jan A. (1 November 2009). "Amylases and bread firming - an integrated ...
This heating process activates enzymes called amylases. The shape of these enzymes, their active site, gives them the unique ...
... enzymes such as amylase; and prostaglandins. Its consistency is determined by the influence of the hormones estrogen and ...
nov., a amylase producing bacterium isolated from a hot spring". Systematic and Applied Microbiology. 28 (5): 415-20. doi: ... Caldimonas taiwanensis produces amylase. Parte, A.C. "Caldimonas". LPSN. "Caldimonas taiwanensis Taxon Passport - StrainInfo". ...
... produces amylase. LPSN lpsn.dsmz.de UniProt Deutsche Sammlung von Mikroorganismen und ... Smith, Michael R.; Zahnley, James C. (2 June 2005). "Production of amylase by Arthrobacter psychrolactophilus". Journal of ... Smith, Michael R.; Zahnley, James C. (19 August 2005). "Characteristics of the amylase of Arthrobacter psychrolactophilus". ... Zellkulturen [1] Smith, Michael R.; Zahnley, James C. (19 August 2005). "Characteristics of the amylase of Arthrobacter ...
The human AMY1C gene encodes the protein Amylase, alpha 1C (salivary). Amylases are secreted proteins that hydrolyze 1,4-alpha- ... This gene encodes an amylase isoenzyme produced by the salivary gland. See also Amylase § Evolution. GRCh38: Ensembl release 89 ... The human genome has a cluster of several amylase genes that are expressed at high levels in either the salivary gland or ... "Entrez Gene: Amylase, alpha 1C (salivary)". Retrieved 2013-02-14. [provided by RefSeq, Jul 2008]. ##RefSeq-Attributes-START## ...
The midgut also contains amylases, roteases and upases. The narrow cells in its reservoir have little to no function in ... which includes proteases and amylases that function as an extraintestinal digestion of solid food. ...
Fischer worked on alpha-amylase. After his PhD, Fischer went to the United States in 1950 for postdoctoral research. He was ...
It is composed of amylase, lipase and protease. This mixture is used to treat conditions in which pancreatic secretions are ... Pancreatic enzymes, also known as pancreases or pancrelipase and pancreatin, are commercial mixtures of amylase, lipase, and ... The trypsin found in pancreatin works to hydrolyze proteins into oligopeptides; amylase hydrolyzes starches into ... "Pancrelipase Lipase; Pancrelipase Protease; Pancrelipase Amylase - Drug Usage Statistics". ClinCalc. Retrieved 11 April 2020. ...
Beta-amylase is present in certain bacteria as well as in plants. Three highly conserved sequence regions are found in all ... Beta-amylase is an enzyme that hydrolyzes 1,4-alpha-glucosidic linkages in starch-type polysaccharide substrates so as to ... The 3D structure of a complex of soybean beta-amylase with an inhibitor (alpha-cyclodextrin) has been determined to 3.0A ... Glycoside hydrolase family 14 CAZY GH_14 comprises enzymes with only one known activity; beta-amylase (EC 3.2.1.2). A Glu ...
Alpha-amylases are found in plants and in animals. Human saliva is rich in amylase, and the pancreas also secretes the enzyme. ... This process is important in the digestion of starch and is also used in brewing, where amylase from the skin of seed grains is ... Marc, A.; Engasser, J. M.; Moll, M.; Flayeux, R. (1983-02-01). "A kinetic model of starch hydrolysis by α- and β-amylase during ... Individuals from populations with a high-starch diet tend to have more amylase genes than those with low-starch diets; Beta- ...
Also of importance is the presence in saliva of the digestive enzymes amylase and lipase. Amylase starts to work on the starch ... Saliva contains digestive enzymes called amylase, and lingual lipase, secreted by the salivary glands and serous glands on the ... The first part of the food to be broken down is the starch of carbohydrates (by the enzyme amylase in the saliva). ... Smaller amounts of lipase and amylase are secreted. The pancreas also secretes phospholipase A2, lysophospholipase, and ...
The Phadebas Amylase Test determines alpha-amylase activity. Organic tests[edit]. *The Carbylamine reaction tests for primary ...
MURAO, Sawao; OOUCHI, Naoki; GOTO, Akira; ARAI, MOTOO (1983). "New proteinaceous .ALPHA.-amylase inhibitor (Paim) from ...
An elevated amylase level, usually > 1,000 IU/L, with protein levels over 3.0 g/dL is diagnostic. Serum amylase is often ...
J. Fukumoto (1943). "Studies on the production of bacterial amylase. I. Isolation of bacteria secreting potent amylases and ... amylase (amylo). Alpha amylase from B. amyloliquefaciens is often used in starch hydrolysis. It is also a source of subtilisin ...
Fiber may affect amylase activity and hence the rate of hydrolysis of starch. The more viscous polysaccharides extend the mouth ... "Amylase-resistant starch plus oral rehydration solution for cholera". The New England Journal of Medicine. 342 (5): 308-13. ... "Amylase-resistant starch as adjunct to oral rehydration therapy in children with diarrhea". Journal of Pediatric ... "A randomized controlled trial of glucose versus amylase resistant starch hypo-osmolar oral rehydration solution for adult ...
Amylase inhibitors, like lipase inhibitors, have been used as a diet aid and obesity treatment. Amylase inhibitors are present ... Amylase inhibitors prevent the action of enzymes that break the glycosidic bonds of starches and other complex carbohydrates, ... Preuss HG (June 2009). "Bean amylase inhibitor and other carbohydrate absorption blockers: effects on diabesity and general ... in many types of beans; commercially available amylase inhibitors are extracted from white kidney beans.[8] ...
Amylases break down the starch in flours into simple sugars, thereby letting yeast ferment quickly. Malt is a natural source of ... Certain other materials may be also added at the mill, notably a-amylase (as malted barley powder or fungal enzyme) and vitamin ... Yeast naturally produces both amylases and proteinases, but additional quantities may be added to produce faster and more ... amylase. Proteases improve extensibility of the dough by degrading some of the gluten. Lipoxygenases oxidize the flour. Other ...
α-Amylase[edit]. Main article: Alpha-amylase. The α-amylases (EC 3.2.1.1 ) (CAS 9014-71-5) (alternative names: 1,4-α-D-glucan ... The optimum pH for β-amylase is 4.0-5.0[4] γ-Amylase[edit]. γ-Amylase (EC 3.2.1.3 ) (alternative names: Glucan 1,4-a- ... β-Amylase[edit]. Another form of amylase, β-amylase (EC 3.2.1.2 ) (alternative names: 1,4-α-D-glucan maltohydrolase; ... Both α-amylase and β-amylase are present in seeds; β-amylase is present in an inactive form prior to germination, whereas α- ...
Amylase finnes i spytt og i sekresjoner fra bukspyttkjertelen. Den starter virkningen på stivelse alt i munnhulen. Stivelse ... Et diagram av et amylase-molekyl fra menneskespytt. Kalsiumionet er synlig i lys khaki, kloridionet i grønt. Fra PDB 1SMD. ... Amylase er en gruppe enzymer som spalter stivelse (polysakkarider) til sukker (disakkarider). ... Et forhøyet nivå av amylase i blod og urin kan være et signal på betennelse i bukspyttkjertelen, såkalt pancreatitt. ...
Amylase is an enzyme that helps digest carbohydrates. It is produced mainly in the pancreas and the glands that make saliva. ... This is a test that measures the amount of amylase in urine. ... Increased urine amylase levels may be a sign of:. *Acute ... This is a test that measures the amount of amylase in urine. Amylase is an enzyme that helps digest carbohydrates. It is ... An increased amount of amylase in the urine is called amylasuria. ...
An amylase test is used to measure levels of amylase in the blood or urine. Abnormal levels may indicate a disorder of the ... What is an amylase test?. An amylase test measures the amount of amylase in your blood or urine. Amylase is an enzyme, or ... An amylase urine test may be ordered along with or after an amylase blood test. Urine amylase results can help diagnose ... Most of your amylase is made in the pancreas and salivary glands. A small amount of amylase in your blood and urine is normal. ...
... amylase: …by the salivary glands, whereas pancreatic amylase is secreted by the pancreas into the small intestine. ... In amylase. …by the salivary glands, whereas pancreatic amylase is secreted by the pancreas into the small intestine. ... Other articles where Pancreatic amylase is discussed: ... Pancreatic amylase. biochemistry. THIS IS A DIRECTORY PAGE. ...
Amylase Vs. Lipase. Of the scores of different enzymes in the body, amylase and lipase are two enzymes that help us digest the ... Amylase Levels in the Blood. Amylase breaks down the starch and carbohydrate present in the food into simple forms that are ... Amylase Test. Amylase is a digestive enzyme that is produced by the salivary gland and the pancreas. This write-up provides ... Salivary Amylase. Salivary amylase, as the name suggests, is secreted in human and animal saliva. The article below explains ...
Both α-amylase and β-amylase are present in seeds; β-amylase is present in an inactive form prior to germination, whereas α- ... In human physiology, both the salivary and pancreatic amylases are α-amylases. The α-amylase form is also found in plants, ... The γ-amylase has most acidic optimum pH of all amylases because it is most active around pH 3. They belong to a variety of ... All amylases are glycoside hydrolases and act on α-1,4-glycosidic bonds. The α-amylases (EC 3.2.1.1 ) (CAS 9014-71-5) ( ...
An amylase test may be done if a child has signs of a problem with the pancreas, such as belly pain, nausea, or vomiting. ... What Is an Amylase Test?. An amylase test measures the amount of amylase in the blood. Amylase is an enzyme made by the ... Why Are Amylase Tests Done?. An amylase test may be done if a child has signs of a problem with the pancreas, such as belly ... Amylase helps the body digest carbohydrates. A high level of amylase in the blood can be a sign that the pancreas is injured, ...
Plasma Amylase After Gastrectomy. Br Med J 1964; 1 doi: https://doi.org/10.1136/bmj.1.5394.1381-a (Published 23 May 1964) Cite ...
Alpha amylase (IPR006046). Short name: Alpha_amylase Overlapping homologous superfamilies *Glycoside hydrolase superfamily ( ... Alpha-amylases, which belong to glycoside hydrolase family 13, are 1,4-alpha-D-glucan glucanohydrolases, which degrade both the ...
Elevated amylase may indicate acute pancreatitis or another pancreatic disease. ... A blood amylase test may be ordered when you have symptoms of a pancreatic disorder. ... P-amylase refers to the type of amylase made mainly in the pancreas. S-amylase refers to the type of amylase made mainly by the ... Measuring pancreatic amylase, or P-amylase, may be useful in determining if an increase in a total amylase level is due to ...
The results showed a low Amylase level of 22 (30-140 being normal range). What does this mean? Is ... ... Low Amylase Levels in Blood Low level of amylase in the body is mostly resulted due to consumption of high amounts of ... Low Amylase Levels in Blood Low level of amylase in the body is mostly resulted due to consumption of high amounts of ... The results showed a low Amylase level of 22 (30-140 being normal range). What does this mean? Is it bad that my amylase level ...
Amylase has to do with the pancreas. Im sure you already know this if youre a nurse. It can mean inflammation, or, it could ... My labs showed elevated amylase at 160 U/L (Labs range is 28-100U/L). My lipase was within range at 32 U/L (Labs range is 13- ...
... ,ARUP Laboratories is a national reference laboratory and a worldwide leader in innovative laboratory ... Infinity Amylase (Powder). 6. Infinity Amylase CNPG3 (Liquid). 7. Infinity Amylase EPS (Liquid). 8. Amylase. 9. Amylase, Body ... Amylase Reagent. 2. Amylase (Colorimetric Method). 3. Amylase (Kinetic Method). 4. Amylase (EPS-G7, kinetics). 5. ...
Beta-amylase (EC 3.2.1.2, β-amylase, saccharogen amylase, glycogenase) is an enzyme with the systematic name 4-alpha-D-glucan ... The optimum pH for β-amylase is 4.0-5.0 They belong to Glycoside hydrolase family 14. Amylase Alpha-amylase Rejzek M, Stevenson ... β-amylase is present in an inactive form prior to seed germination. Many microbes also produce amylase to degrade extracellular ... "Amylase, Alpha" , I.U.B.: 3.2.1.11,4-α-D-Glucan glucanohydrolase. Beta-amylase at the US National Library of Medicine Medical ...
Record start and stop time and date on the requisition. Instruct patient on proper collection. At the beginning of collection have patient void and discard this urine. Begin timing collection at this point and collect all urine until the end of the collection period. Have patient void and save urine at the final collection point.. ...
Amylase increased answers are found in the Diagnosaurus powered by Unbound Medicine. Available for iPhone, iPad, Android, and ... 5minute, www.unboundmedicine.com/5minute/view/Diagnosaurus/114728/all/Amylase_increased. Zeiger RFR. Amylase increased. ... Amylase increased is a topic covered in the Diagnosaurus. To view the entire topic, please log in or purchase a subscription. ... Zeiger, Roni F.. "Amylase Increased." Diagnosaurus, 4th ed., McGraw-Hill Education, 2015. ...
For the quantitative kinetic determination of amylase activity, using manual or automated procedures, in human serum and urine ... Infinity Amylase CNPG3 (Liquid). 6. Infinity Amylase EPS (Liquid). 7. Amylase. 8. Amylase, Body Fluid. 9. Amylase, Isoenzymes. ... Amylase Reagent. 2. Amylase (Colorimetric Method). 3. Amylase (EPS-G7, kinetics). 4. Infinity Amylase (Powder). 5. ... For the quantitative kinetic determination of amylase activity, using manual or automated procedures, in human serum and urine. ...
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Amylase is an enzyme that catalyses the breakdown of starch into sugars. Amylases are f… ... Investigate the effect of temperature on amylase activityIntroduction ... Amylases are foundin almost all plants, animals and microorganisms. Large amounts of amylase occur in germinatingcereals, and ... If the volume of amylase solution is same ineach test tube, the lower concentration means that there are less amylase molecules ...
Amylase enters the bloodstream when the pancreas becomes inflamed... ... The pancreas and the glands that produce saliva also produce amylase, an enzyme that helps the body digest food, notes ... What does the amylase test indicate for the pancreas?. A: The amylase test indicates whether the pancreas is damaged or blocked ... What is the function of salivary amylase?. A: Salivary amylase is an enzyme responsible for breaking down starch, according to ...
... Subash C. B. Gopinath,1,2 Periasamy Anbu,3 M. K. Md Arshad,1 ... Amylase is an important and indispensable enzyme that plays a pivotal role in the field of biotechnology. It is produced mainly ... Herein, the importance of microbial (bacteria and fungi) amylase is discussed along with its production methods from the ... Industrial sectors with top-down and bottom-up approaches are currently focusing on improving microbial amylase production ...
IPR031319. A-amylase_C. IPR006046. Alpha_amylase. IPR031965. CBM26. IPR006047. Glyco_hydro_13_cat_dom. IPR013780. Glyco_hydro_b ... IPR031319. A-amylase_C. IPR006046. Alpha_amylase. IPR031965. CBM26. IPR006047. Glyco_hydro_13_cat_dom. IPR013780. Glyco_hydro_b ... Alpha-amylaseImported. ,p>Information which has been imported from another database using automatic procedures.,/p> ,p>,a href ... tr,Q48502,Q48502_LACAM Alpha-amylase OS=Lactobacillus amylovorus OX=1604 PE=3 SV=2 ...
Make research projects and school reports about amylase easy with credible articles from our FREE, online encyclopedia and ... amylase Digestive enzyme secreted by the salivary glands (salivary amylase) and the pancreas (pancreatic amylase. It aids ... as pancreatic amylase) and also (in humans and some other species) in saliva (as salivary amylase or ptyalin). Amylases cleave ... Plants contain both α- and β-amylases; the name diastase is given to the component of malt containing β-amylase, important in ...
Find information on amylase use, treatment, drug class and molecular formula. ... Lists the various brand names available for medicines containing amylase. ... Medications containing amylase:. Multi-ingredient medications containing amylase:. amylase/cellulase/hyoscyamine/lipase/ ... Amylase. Important: The information below refers to medicines available in the United States that contain amylase. ...
PS00506. BETA_AMYLASE_1. 1 hit. PS00679. BETA_AMYLASE_2. 1 hit. ... PS00506. BETA_AMYLASE_1. 1 hit. PS00679. BETA_AMYLASE_2. 1 hit ... Beta-amylaseUniRule annotation. ,p>Information which has been generated by the UniProtKB automatic annotation system, without ... tr,Q42989,Q42989_ORYSA Beta-amylase OS=Oryza sativa OX=4530 PE=3 SV=1 ...
... BruceRat brucerat at aol.com Mon Oct 23 19:25:25 EST 1995 *Previous message: ... Using a simple photometer to detect digestion of iodine-stained arrowroot starch by salivary amylase, I have gotten a graph ...
VIATREXX-ENZYME PLUS (amylase, cellulase, lactase, lipase, maltase, protease, sucrase) spray. NDC Code(s): 63776-142-11, 63776- ... PANCREAZE (pancrelipase lipase, pancrelipase amylase, and pancrelipase protease) capsule, delayed release. NDC Code(s): 62541- ... ZENPEP (pancrelipase lipase, pancrelipase protease, pancrelipase amylase) capsule, delayed release. NDC Code(s): 0023-6110-01, ... ZENPEP (pancrelipase lipase, pancrelipase protease, pancrelipase amylase) capsule, delayed release. NDC Code(s): 73562-110-01, ...
amylase isoenzymes synonyms, amylase isoenzymes pronunciation, amylase isoenzymes translation, English dictionary definition of ... amylase isoenzymes. n. Any of a group of enzymes that catalyze the hydrolysis of starch to sugars. In humans, amylases are ... amylase. (redirected from amylase isoenzymes). Also found in: Thesaurus, Medical, Encyclopedia. am·y·lase. (ăm′ə-lās′, -lāz′). ... Amylase isoenzymes - definition of amylase isoenzymes by The Free Dictionary https://www.thefreedictionary.com/amylase+ ...
Amylase and Hypersomnia (Amylase). The safety and scientific validity of this study is the responsibility of the study sponsor ... Show an increase of salivary amylase for children with hypersomnia or narcolepsy compared to a group of children matched on age ... Studies have shown for healthy subjects, that the expression and activity of salivary amylase are increased when subjects are ... Evaluation of Excessive Diurnal Sleepiness by the Expression and Activity of Salivary Amylase in Children With Hypersomnia.. ...
  • Amylase is present in the saliva of humans and some other mammals, where it begins the chemical process of digestion . (wikipedia.org)
  • Salivary amylase, as the name suggests, is secreted in human and animal saliva. (buzzle.com)
  • Large amounts of amylase occur in germinatingcereals, and in the pancreas and saliva of higher animals.AimThe aim of this experiment is to find out the rate of reaction between amylase and starch in arange of different reaction temperatures.Hypothesis As the reaction temperature of amylase solution and starch solution increase, the reaction rateof amylase and starch will increase. (slideshare.net)
  • The pancreas and the glands that produce saliva also produce amylase, an enzyme that helps the body digest food, notes MedlinePlus. (reference.com)
  • Animals possess only α-amylases, found in pancreatic juice (as pancreatic amylase ) and also (in humans and some other species) in saliva (as salivary amylase or ptyalin ). (encyclopedia.com)
  • Amylase is present in fluid secreted by the pancreas, in saliva in some mammals, and in plants. (thefreedictionary.com)
  • Amylase is a digestive enzyme produced mostly by the pancreas and saliva glands. (wisegeek.com)
  • Amylase is an enzyme that breaks down starch and it is secreted in saliva. (wisegeek.com)
  • Anti-α-amylase antibody reacts specifically with α amylase present in human saliva and pancreatic extract but not with other proteins of saliva and pancreatic extract. (sigmaaldrich.com)
  • The antiserum is specific for human a-amylase found in human saliva and human pancreatic extract. (sigmaaldrich.com)
  • Some case studies suggest a potential link between higher-than-normal levels of amylase, particularly in the saliva, and ovarian tumors. (medicalnewstoday.com)
  • Medical Dictionary states that salivary amylase is an enzyme produced by the saliva glands to catalyse the hydrolysis, or breaking down, of starch into smaller, simpler compounds. (reference.com)
  • Salivary amylase, also referred to as ptyalin, is the name of the enzyme found in saliva. (reference.com)
  • The enzyme present in our saliva is called Amylase. (ipl.org)
  • Amylase is a type of digestive enzyme found in the pancreases and saliva of humans. (ipl.org)
  • Amylase Is An Enzyme Commonly Found In Human Saliva. (bartleby.com)
  • Amylase is found in one's saliva and pancreas, and Porcrine Pancreatic α-Amylase is amylase that has been isolated from the pancreas of a pig. (bartleby.com)
  • While amylase is effective at breaking down starch , in the normal saliva pH range of 6.02 to 7.14 (Schmidt-Nielsen, 1946), any pH environment outside of this range will decrease the reaction rate. (bartleby.com)
  • Digestion of your dietary intake of carbohydrates and fat relies on the action of amylase, found in the saliva, to begin the digestion of starches. (chemocare.com)
  • Amylase is an enzyme which is present in the human saliva. (medindia.net)
  • Salivary Amylase and Other Enzymes in Saliva, Regina Bailey is a board-certified registered nurse, science writer and educator. (diobeth.org)
  • Salivary amylase is the primary enzyme in saliva. (diobeth.org)
  • Healthy, nonobese individuals were prescreened for salivary amylase activity and classified as high (HA) or low amylase (LA) if their activity levels per minute fell 1 SD higher or lower than the group mean… This enzyme is found in the saliva produced by the salivary glands located in and around the mouth. (diobeth.org)
  • Amylases are found in the saliva of most mammals (and were previously called ptyalin). (diobeth.org)
  • They are produced by the pancreas and salivary glands, being found in saliva in the form of alpha-amylase (also called ptyalin) and in the pancreas as pancreatic amylase. (diobeth.org)
  • For those of you who haven't heard of it, amylase is an enzyme we have in our saliva and somewhere below the stomach. (veganforum.com)
  • Amylase is a very important starch- metabolizing enzyme that is produced in both the saliva and in the pancreas to speed the digestive process. (organicfacts.net)
  • If your health care provider suspects you have pancreatitis, he or she may order a lipase blood test , along with an amylase blood test. (medlineplus.gov)
  • Of the scores of different enzymes in the body, amylase and lipase are two enzymes that help us digest the food we eat. (buzzle.com)
  • The blood amylase test may be used along with a lipase test to detect pancreatic diseases. (labtestsonline.org)
  • My labs showed elevated amylase at 160 U/L (Lab's range is 28-100U/L). My lipase was within range at 32 U/L (Lab's range is 13-60 U/L). I had the MRI on 5/22/14. (medhelp.org)
  • Elevated pancreatic enzymes such as amylase and lipase can indicate pancreatitis, a serious inflammation of the pancreas that causes these enzymes to be si. (reference.com)
  • Liver function tests, lipase enzyme tests, and additional blood tests can help pinpoint the cause of high or low amylase levels. (wisegeek.com)
  • Amylase and lipase tests are useful in diagnosing or monitoring tumors and cancers that affect the pancreas. (medicalnewstoday.com)
  • Ethanol extracts, dried powders and fibres (total and soluble fibre) of the tropical red algae Kappaphycus alvarezii , Kappaphycus striatus and Eucheuma denticulatum were analysed for their effect on lipase and α-amylase activity using turbidimetric method and dinitrosalicylic acid (DNS) assay, respectively. (springer.com)
  • Blood amylase and lipase levels are most frequently drawn to diagnose pancreatitis. (chemocare.com)
  • This can cause amylase and lipase levels to be increased up to 3 times the normal limit. (chemocare.com)
  • Read user comments about the side effects, benefits, and effectiveness of amylase-lipase-protease-cellulase oral. (webmd.com)
  • Although three key enzymes - lipase, protease and amylase - are important in managing diabetes because they will help digest proteins, fats and sugars, research is showing amylase as the key enzyme for managing diabetes. (jonbarron.org)
  • In the detergent industry [12], α-amylase acts synergistically with alkaline protease, lipase, etc, to give it a better wash. (scirp.org)
  • The α-amylase form is also found in plants, fungi ( ascomycetes and basidiomycetes ) and bacteria ( Bacillus ). (wikipedia.org)
  • The crystals of beta-amylase from Bacillus cereus belong to space group P21 with the following cell dimensions: a = 57.70 A, b = 92.87 A, c = 65.93 A, and beta =101.95 degrees. (rcsb.org)
  • Crystal structure of calcium-depleted Bacillus licheniformis alpha-amylase at 2.2 A resolution. (ebi.ac.uk)
  • Molecular genetic methods used to clone and express the Bacillus stearothermophilus maltogenic amylase gene 4. (inchem.org)
  • EXPLANATION This enzyme preparation is a maltogenic amylase produced by submerged fermentation of a non-pathogenic and non-toxicogenic strain of Bacillus subtilis which, by recombinant DNA techniques, contains the amy M gene from Bacillus stearothermophilus coding for maltogenic amylase. (inchem.org)
  • Section: U29 ABSTRACT The effects of temperature on fungal amylase Aspergillus oryzae, and bacterial amylase, Bacillus licheniformis ability to break down starch into maltose was studied. (bartleby.com)
  • α-Amylase purified from Bacillus sp. (bio-medicine.org)
  • Bacteria such as Bacillus (which incidentally also make the enzymes found in detergents), and fungi such as Asperigilus (bread mould) are typically used for amylase production. (veganforum.com)
  • In this study, Bacillus subtilis QM3 has ability to produce α-amylase. (scirp.org)
  • Hu, Q.P. and Liu, J.J. (2021) Production of α-Amylase by Bacillus subtilis QM3 and its Enzymatic Properties. (scirp.org)
  • Bacillus species is considered the best producers of α-amylase that play essential roles in starch processing industry [15]. (scirp.org)
  • Amylases isolated from different Bacillus species have significantly different properties, such as the optimum pH and temperature as well as metal ion requirements [15]. (scirp.org)
  • In this study, taking Bacillus subtilis QM3 as the research object, production of α-amylase by B . subtilis QM3 was identification by iodine test. (scirp.org)
  • CARBOHYDRASE (alpha-AMYLASE) FROM BACILLUS LICHENFORMIS EXPLANATION Carbohydrase is an enzyme that catalyzes the hydrolysis of alpha-1,4-glycosidic linkages of starch. (inchem.org)
  • Food-Grade Thermostable Alpha- Amylase HT-200 Stable Activity, Low pH Tolerate, Effective Liquefaction Amylase Halal & Kosher Certified PRODUCT DESCRIPTON: HT-200 is low pH tolerated Thermostable Alpha- Amylase derived from Bacillus licheniformis. (splicd.com)
  • Amylase is a digestive enzyme that is produced by the salivary gland and the pancreas. (buzzle.com)
  • Amylase is a digestive enzyme that helps the body break down carbohydrates . (medicalnewstoday.com)
  • Pancreatic amylase is a digestive enzyme secreted by the pancreas. (abcam.com)
  • Starches are complex carbohydrates that cannot be absorbed unless they are first broken down by the digestive enzyme amylase and other, secondary, enzymes. (peacehealth.org)
  • Substances that inhibit amylase, the digestive enzyme required to break down dietary starches into absorbable glucose units, can reduce the usual post-meal rise in blood sugar levels in both healthy people and people with diabetes. (peacehealth.org)
  • Amylase inhibitors are substances that reduce the activity of amylase, the digestive enzyme required to break down dietary starches into absorbable glucose. (peacehealth.org)
  • Amylase can typically be found in many enzyme supplements, most specifically digestive enzyme formulas. (jonbarron.org)
  • Partially purified enzyme preparation with specific activities of 153.7 U/mg for α-amylase and 0.15 U/mg for protease was produced by selective adsorption on starch. (springer.com)
  • Enzymes were purified until homogeneous electrophoretically by gel-filtration over HW-55 TSK-gel with specific activities of 245 U/mg for α-amylase and 1.44 U/mg for protease. (springer.com)
  • What Is Serum Amylase? (wisegeek.com)
  • Serum amylase is the measure of amylase in the blood. (wisegeek.com)
  • Serum amylase tests sometimes produce falsely high readings in healthy individuals as well as normal readings in patients with pancreatic diseases. (wisegeek.com)
  • Some of these conditions, such as obstruction of the bile duct , cause a temporary rise of serum amylase. (wisegeek.com)
  • The resultant readings of a serum amylase test should not be compared to the amylase values of a different facility. (wisegeek.com)
  • A 2013 study published in the Journal of Clinical and Diagnostic Research found that in type 2 diabetes, whenever the blood glucose level was higher, the serum amylase activity was found to be "significantly" lower-reflecting dysfunction in the pancreas. (jonbarron.org)
  • About 80% of subjects with acute pancreatitis have increased serum amylase within 24 hours. (lifeextension.com)
  • By acting at random locations along the starch chain, α-amylase breaks down long-chain saccharides , ultimately yielding either maltotriose and maltose from amylose , or maltose, glucose and "limit dextrin" from amylopectin . (wikipedia.org)
  • Working from the non-reducing end, β-amylase catalyzes the hydrolysis of the second α-1,4 glycosidic bond, cleaving off two glucose units ( maltose ) at a time. (wikipedia.org)
  • During the ripening of fruit , β-amylase breaks starch into maltose, resulting in the sweet flavor of ripe fruit. (wikipedia.org)
  • Amylase spalter amylosen, som er lange sukkerkjeder, til to og to glukoseenheter (disakkarider) av gangen, slik at det kan dannes maltose . (wikipedia.org)
  • Amylase hydrolyzes starch , glycogen , and dextrin to form in all three instances glucose , maltose , and the limit-dextrins. (encyclopedia.com)
  • Amylases cleave the glycosidic bonds of the long polysaccharide chains, producing a mixture of glucose and maltose. (encyclopedia.com)
  • The structures of free and maltose-bound beta-amylases were determined by X-ray crystallography at 2.1 and 2.5 A with R-factors of 0.170 and 0.164, respectively. (rcsb.org)
  • The structure of the core domain is similar to that of soybean beta-amylase except for the L4 maltose-binding site, whereas the C-terminal domain has the same secondary structure as domain E of cyclodextrin glucosyltransferase. (rcsb.org)
  • These results indicate that the ability of B. cereus beta-amylase to digest raw starch can be attributed to the additional two maltose-binding sites. (rcsb.org)
  • Amylase is an enzyme that breaks up the glucose chains in starch into maltose. (bartleby.com)
  • This β‐amylase is intended to be used in the starch processing for maltose syrup production and the manufacture of a Japanese rice cake type. (europa.eu)
  • α-Amylase is a hydrolytic enzyme that catalyzes the conversion of starch to a mixture of glucose, maltose, maltotriose and dextrins. (bio-medicine.org)
  • Salivary amylase breaks starch into a disaccharide called maltose. (diobeth.org)
  • ase - endelse som angir at det er et enzym) - En gruppe enzymer som bryter ned (hydrolyserer) stivelse til mindre bestanddeler (oligosakkarider, dekstriner, maltose, glukose). (uio.no)
  • Beta-amylase (β-amylase)spalter av beta-maltose fra den reduserende enden av stivelsesmolekylet. (uio.no)
  • The pancreas and salivary gland make amylase ( alpha amylase ) to hydrolyse dietary starch into disaccharides and trisaccharides which are converted by other enzymes to glucose to supply the body with energy. (wikipedia.org)
  • Alpha-amylases, which belong to glycoside hydrolase family 13, are 1,4-alpha-D-glucan glucanohydrolases, which degrade both the branched and unbranched forms of starch by cleaving the internal alpha-1,4 bonds connecting the glucose monomers. (ebi.ac.uk)
  • It is an exo-acting maltogenic amylase enzyme (E.C.3.2.1.133, glucan alpha-1,4-maltohydrolase) and catalyses the hydrolysis of alpha-1,4-glucosidic linkages in amylose, amylopectin and related glucose polymers. (inchem.org)
  • This α‐amylase is intended to be used in starch processing for glucose syrups production, beverage alcohol (distilling) processes and baking processes. (europa.eu)
  • α-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. (sigmaaldrich.com)
  • Amylase (alpha amylase) is also present in pancreas and this aids in hydrolyzing the starch in our diet into disaccharides and trisaccharides, which are eventually converted into glucose by other enzymes. (medindia.net)
  • Amylase inhibitors from various medicinal herbs and plant foods may reduce the usual after-meal rise in blood glucose levels in people with diabetes. (peacehealth.org)
  • For example, adding beans to a serving of rice reduces the rise in blood glucose, an effect that may be due in part to the amylase-inhibiting action of beans. (peacehealth.org)
  • A review of clinical trials reported that taking 1,500 to 3,000 mg of an amylase-inhibiting extract from white beans (Phaseolus vulgaris) before meals has been shown in multiple studies to reduce post-meal blood glucose levels and may promote weight loss. (peacehealth.org)
  • Blocking alpha-amylase inhibits the breakdown of starches and slows the rate at which free sugars are absorbed, thereby blunting glucose spikes. (thefreedictionary.com)
  • At this point, it is referred to as pancreatic amylase and it works to complete the process of digesting carbohydrates and producing glucose. (jonbarron.org)
  • Well, a key function of amylase is to break down the carbs into available forms of energy (glucose) for your body. (jonbarron.org)
  • Extracts of two varieties of M. charantia have α-amylase and α-glucosidase inhibiting activities in vitro and glucose lowering activity in vivo. (greenmedinfo.com)
  • α-amylase and α-glucosidase digest the carbohydrates and increase the postprandial glucose level in diabetic patients. (greenmedinfo.com)
  • muricata (MCM) were tested for α-amylase and α-glucosidase inhibiting activities in vitro, and glucose lowering activity after oral administration in vivo. (greenmedinfo.com)
  • Protein extracts from the fruits of the two varieties of bitter gourd inhibitedα-amylase and α-glucosidase in vitro and lowered the blood glucose level in vivo on par with Acarbose when orally administrated to Streptozotocin-induced diabetic rats. (greenmedinfo.com)
  • Amylase is an enzyme that helps digest carbohydrates. (medlineplus.gov)
  • Amylase helps the body digest carbohydrates . (kidshealth.org)
  • Amylase is an enzyme produced primarily by the pancreas and the salivary glands to help digest carbohydrates. (labtestsonline.org)
  • The pancreas releases amylase through the pancreatic duct into the first part of the small intestine, where it helps break down dietary carbohydrates. (labtestsonline.org)
  • Low level of amylase in the body is mostly resulted due to consumption of high amounts of carbohydrates. (medhelp.org)
  • As mentioned earlier, amylase catalyzes starch and complex carbohydrates. (medhelp.org)
  • Several enzymes are involved in the breakdown of carbohydrates, including salivary amylase, pancreatic amylase, maltase, sucrase and lactase. (reference.com)
  • Amylase inhibitors contain substances that prevent dietary carbohydrates from being absorbed by the body and may aid in weight loss. (peacehealth.org)
  • Salivary amylase breaks down carbohydrates into smaller molecules, like sugars. (diobeth.org)
  • However, only a few specialized supplement companies understand its powerful use in a (systemic) proteolytic enzyme formula, such as its use in Jon Barron's pHi-Zymes formula , where amylase complements the action of the proteolytics by working on leftover carbohydrates both in the intestinal tract and the bloodstream-cleaning up what digestive enzymes fail to touch. (jonbarron.org)
  • As for amylase, it cleans up the carbohydrates in the bloodstream that can lead to sugar imbalances. (jonbarron.org)
  • This is a test that measures the amount of amylase in urine. (medlineplus.gov)
  • An increased amount of amylase in the urine is called amylasuria. (medlineplus.gov)
  • An amylase test measures the amount of amylase in your blood or urine. (medlineplus.gov)
  • A small amount of amylase in your blood and urine is normal. (medlineplus.gov)
  • This test measures the amount of amylase in the blood or urine or sometimes in peritoneal fluid , which is fluid found between the membranes that cover the abdominal cavity and the outside of abdominal organs. (labtestsonline.org)
  • The Amount of amylase/starch used were kept to 5cm3 at all times. (ipl.org)
  • saccharogen amylase) is also synthesized by bacteria , fungi , and plants . (wikipedia.org)
  • Beta-amylase (EC 3.2.1.2, β-amylase, saccharogen amylase, glycogenase) is an enzyme with the systematic name 4-alpha-D-glucan maltohydrolase. (wikipedia.org)
  • Your health care provider may order an amylase blood and/or urine test if you have symptoms of a pancreatic disorder. (medlineplus.gov)
  • To help diagnose pancreatitis, a doctor may order an amylase blood test. (medicalnewstoday.com)
  • An review of available dataindicates that alpha-amylase is worst case scenario within aquatic toxicity and aquatic toxicity data obtained with alpha-amylase hence cover data for non-proteolytic enzymes in general. (europa.eu)
  • 1. Investigate the effect of temperature on amylase activityIntroduction Amylase is an enzyme that catalyses the breakdown of starch into sugars. (slideshare.net)
  • In the digestive systems of humans and many other mammals, an alpha-amylase called ptyalin is produced by the salivary glands, whereas pancreatic amylase is secreted by the pancreas into the small intestine. (diobeth.org)
  • An amylase ( / ˈ æ m ɪ l eɪ z / ) is an enzyme that catalyses the hydrolysis of starch (Latin amylum ) into sugars . (wikipedia.org)
  • α- and β-amylases are important in brewing beer and liquor made from sugars derived from starch . (wikipedia.org)
  • An amylase (/ˈæmɪleɪz/) is an enzyme that catalyses the hydrolysis of starch (Latin amylum) into sugars. (wikipedia.org)
  • In traditional beer brewing, malted barley is mixed with hot water to create a "mash", which is held at a given temperature to allow the amylases in the malted grain to convert the barley's starch into sugars. (wikipedia.org)
  • Different temperatures optimize the activity of alpha or beta amylase, resulting in different mixtures of fermentable and unfermentable sugars. (wikipedia.org)
  • Amylases are used in breadmaking and to break down complex sugars, such as starch (found in flour), into simple sugars. (wikipedia.org)
  • In order for the yeast to make those lovely bubbles (carbon dioxide) in your bread from simple sugars, you have to either rise for a long time or provide some amylases so that there's adequate digestion of starch into simple sugars. (veganforum.com)
  • Hi everyone, I'm experimenting with alpha amylase to increase the fermentability of malt extracts by breaking down some of the complex sugars and other hard-to-ferment components. (homebrewtalk.com)
  • Quote from BeerSmith's page: A low step temperature (146-150F/63-66 C) emphasizing beta amylase will therefore result in a more complete conversion to simple sugars, but will. (homebrewtalk.com)
  • Aquatic toxicity data for a variety of non-proteolytic enzyme clasases are available among those for alpha-amylase, cellulase and cellulase and data show that non-proteolytic enzymes in general are non- or of low toxicity towards aquatic organisms. (europa.eu)
  • Herein, the importance of microbial (bacteria and fungi) amylase is discussed along with its production methods from the laboratory to industrial scales. (hindawi.com)
  • Amylases are mostly produced by fungi or bacteria on large scales, as it is much much much much more economical than extracting them from animals. (veganforum.com)
  • http://www.worthington-biochem.com/BA/default.html "Amylase, Alpha" , I.U.B.: 3.2.1.11,4-α-D-Glucan glucanohydrolase. (wikipedia.org)
  • Enzymes such as amylase are very important in digestion since humans cannot directly absorb starch and other large, complex molecules. (reference.com)
  • Some European studies have shown that enzymes such as amylase may be effective in reducing swelling in some conditions such as rheumatic diseases. (jonbarron.org)
  • Scientists are currently exploring the strong possibility that enzymes such as amylase can help slow the molecules that trigger immune responses and in addition, slow the tissue damage created from these responses. (jonbarron.org)
  • Bisdemethoxycurcumin from Curcuma longa rhizome is a potent small molecule inhibitor of human pancreatic α-amylase, a target for type-2 diabetes. (greenmedinfo.com)
  • This was transformed from an inhibitor of human pancreatic alpha-amylase with a K(i) value of 18 mm to a trisaccharide analogue with a K(i) value of 25 mum. (nih.gov)
  • The article below explains the functions of the salivary amylase and the role it plays in the process of digestion. (buzzle.com)
  • Using a simple photometer to detect digestion of iodine-stained arrowroot starch by salivary amylase, I have gotten a graph that is FAIRLY closely modeled by the Michaelis-Menten equation. (bio.net)
  • Alpha amylase is a protein involved in the digestion of food. (wisegeek.com)
  • The pancreas also secretes amylase to aid in digestion. (reference.com)
  • Like amylase, it helps with the digestion process. (diobeth.org)
  • Supplement The amylase is produced and secreted by salivary glands and pancreas to aid in the chemical digestion of foodstuff (such as starch). (diobeth.org)
  • Conditions affecting the kidneys or liver may also result in high blood amylase results. (wisegeek.com)
  • Avoiding alcohol, and taking all the prescribed medications are the primary treatments for high blood amylase levels, if you are being followed in the outpatient department, and you have not been diagnosed with any kind of pancreatitis. (chemocare.com)
  • If you are experiencing high blood amylase levels, and are without symptoms, you doctor or healthcare provider may tell you to avoid alcohol and narcotics, and they may closely monitor your laboratory values and symptoms. (chemocare.com)
  • A urine amylase test may also be used to provide a wider range of diagnostic information. (wisegeek.com)
  • For urine amylase test, a 24- hours urine sample needs to be collected. (medindia.net)
  • Certain medications can affect amylase levels, so discuss medications with your doctor before testing is done. (labtestsonline.org)
  • Several different medical conditions can affect amylase levels in the blood. (medicalnewstoday.com)
  • A variety of medical conditions can affect amylase levels in the blood. (medicalnewstoday.com)
  • Cigarette smoking and salivary amylase activity. (bmj.com)
  • Three human α-amylases exist: Amy1 (salivary amylase), Amy2A (pancreatic amylase), and Amy2B (expressed in various tissues). (scirp.org)
  • Amy2B also is a pancreatic amylase but it is expressed in various tissues. (scirp.org)
  • Amylase is an enzyme, or special protein, that helps you digest food. (medlineplus.gov)
  • alpha amylase, catalytic domain protein [Capnocytophaga sp. (nih.gov)
  • In vitro and in vivoα-amylase and α-glucosidase inhibiting activities of the protein extracts from two varieties of bitter gourd (Momordica charantia L. (greenmedinfo.com)
  • The protein extract from both MCC and MCM inhibited the activity ofα-amylase and α-glucosidase through competitive inhibition, which was on par with Acarbose as indicated by in vitro percentage of inhibition (66 to 69 %) and IC50 (0.26 to 0.29 mg/ml). (greenmedinfo.com)
  • The EnzChek Amylase Assay Kit (E-11954) provides the speed, high sensitivity and convenience required for measuring a-amylase activity or for screening inhibitors in a high-throughput format. (bio-medicine.org)
  • Amylase inhibitors are also known as starch blockers because they contain substances that prevent dietary starches from being absorbed by the body. (peacehealth.org)
  • 3 , 4 , 5 , 6 Later, however, highly concentrated versions of amylase inhibitors did show potential for reducing carbohydrate absorption in humans. (peacehealth.org)
  • Amylase inhibitors, taken with meals, may reduce the usual rise in blood sugar levels in people with diabetes. (peacehealth.org)
  • Amylase inhibitors occur naturally in foods such as whole grains and legumes, as well as in many culinary herbs and spices and medicinal herbs, possibly contributing to their anti-diabetic effects. (peacehealth.org)
  • Depending on the potency of the amylase inhibitors , typical intake is 1,500 to 6,000 mg before meals. (peacehealth.org)
  • A new approach for the discovery and subsequent structural elucidation of oligosaccharide-based inhibitors of alpha-amylases based upon autoglucosylation of known alpha-glucosidase inhibitors is presented. (nih.gov)
  • This approach to the discovery and structural analysis of amylase inhibitors should be generally applicable to other endoglucosidases and readily adaptable to a high throughput format. (nih.gov)
  • For the quantitative kinetic determination of amylase activity, using manual or automated procedures, in human serum and urine. (bio-medicine.org)
  • Amylase activity-Michaelis-Menten modelling? (bio.net)
  • However, the salivary amylase isoenzyme (S-type) interferes in the analyses and results in poor specificity for tests of total amylase activity [9]. (thefreedictionary.com)
  • Studies have shown for healthy subjects, that the expression and activity of salivary amylase are increased when subjects are deprived of sleep. (clinicaltrials.gov)
  • Determination of the expression and enzymatic activity of salivary amylase. (clinicaltrials.gov)
  • What is the effect of temperature Amylase activity? (ipl.org)
  • Tea inhibited the activity of salivary amylase. (greenmedinfo.com)
  • The optimum temperature and pH for purified α-amylase activity are 40-50°C and pH 6.0. (springer.com)
  • even though there is no true consensus on the exact optimum pH for alpha-amylase activity, all of the sources place it around 6.00 or 7.00. (bartleby.com)
  • The Effect Of Ph Levels On The Rate Of Activity Of ⍺-Amylase. (bartleby.com)
  • The Effect of pH levels on the Rate of Activity of ⍺-Amylase Ryan Andrew Introduction: Enzymes are some of the most important chemicals in the human body. (bartleby.com)
  • Salivary Alpha-Amylase Activity, a New Biomarker in Heart Failure? (diva-portal.org)
  • Salivary α-amylase activity is an increasingly investigated biomarker for the activation of the autonomic nervous system. (diva-portal.org)
  • Autonomic imbalance is associated to several diseases, one of which is heart failure, and the aim of the present study was to test if salivary α-amylase activity might be a new biomarker in patients with chronic heart failure. (diva-portal.org)
  • Results: Within the whole group of participants, a statistically significant positive correlation between morning salivary α-amylase activity levels and serum NT-proBNP could be found, which was strongest for the measurement taken 30 minutes after awakening, as well as a significant negative correlation of awakening α-amylase activity levels with arterial blood pressure. (diva-portal.org)
  • Within the control group separately, higher daily salivary α-amylase activity output correlated with increasing levels of NT-proBNP, while within the patients, the strongest association of α-amylase activity measures were found to be a negative correlation with blood pressure. (diva-portal.org)
  • The accompanying increase in fluorescence is proportional to amylase activity and can be monitored with a fluorescence microplate reader, minifluorometer or standard fluorometer. (bio-medicine.org)
  • Measurement of serum α-amylase activity is an important diagnostic test for acute and chronic pancreatitis. (diobeth.org)
  • Alpha-amylase activity was determined by Bernfeld method [5]. (thefreedictionary.com)
  • Human salivary alpha-amylase Trp58 situated at subsite-2 is critical for enzyme activity. (thefreedictionary.com)
  • Alpha-amylase activity was measured using 3,5-dinitrosalicylic acid (DNS). (scirp.org)
  • The result showed B. subtilis QM3 can produce α-amylase and activity of α-amylase was 50.58 IU/mL. (scirp.org)
  • Ca 2+ , Na + , K + and Mg 2+ promoted the activity of alpha-amylase, and their relative enzyme activities were 130.1%, 118.1%, 115.9% and 110.4% respectively. (scirp.org)
  • Fe 2+ , Zn 2+ , Cu 2+ , Mn 2+ and EDTA inhibited the activity of alpha-amylase. (scirp.org)
  • The amylase activity of B. subtilis QM3 was determined by 3,5-dinitrosalicylic acid (DNS), and the enzymatic properties were studied. (scirp.org)
  • If your results show an abnormal level of amylase in your blood or urine, it may mean you have a disorder of the pancreas or other medical condition. (medlineplus.gov)
  • A high level of amylase in the blood can be a sign that the pancreas is injured, irritated, or blocked. (kidshealth.org)
  • Eventually, the level of amylase falls down from the normal recommended level, thus manifesting amylase deficiency symptoms. (medhelp.org)
  • Low level of amylase is also related to other skin problems such as inflammation, allergic reactions, eczema, psoriasis, and atopic dermatitis. (medhelp.org)
  • A low level of amylase can also be indicative of a medical condition. (wisegeek.com)
  • In this study, we evaluated the glycosylation state of human amylase isozymes and found that the different glycosylation patterns of human amylases were related to the types of amylase-producing cells rather than the types of amylase isozymes. (scirp.org)
  • When cells in the pancreas are injured, increased amounts of amylase are released into the blood. (labtestsonline.org)
  • In addition to salivary amylase, humans also produce pancreatic amylase, which further breaks down starches later in the digestive process. (diobeth.org)
  • An amylase blood test is used to diagnose or monitor a problem with your pancreas, including pancreatitis , an inflammation of the pancreas. (medlineplus.gov)
  • Increased amylase levels can occur with pancreatitis or when the pancreatic duct is blocked by a gallstone or, in rare cases, with a pancreatic tumor. (labtestsonline.org)
  • The blood amylase test is used to help diagnose and monitor acute pancreatitis . (labtestsonline.org)
  • Severe cases of amylase deficiency are detected in patients having chronic pancreatitis, cirrhosis, pancreatic cancer, kidney disease, and hepatitis. (medhelp.org)
  • Doctors may order an amylase test, in which samples of either blood or urine are taken, for patients who have or display the symptoms of acute pancreatitis, chronic pancreatitis or pancreatic pseuodocysts. (reference.com)
  • Abnormal levels of amylase may indicate pancreatitis or another problem with the pancreas. (medicalnewstoday.com)
  • Doctors commonly use amylase blood tests to diagnose or monitor people with acute pancreatitis . (medicalnewstoday.com)
  • Abnormally high or low levels of amylase in the blood can be a sign that a person has pancreatitis. (medicalnewstoday.com)
  • Measurement of amylase concentrations in serum is a diagnostic test for various pancreatic disorders (especially acute and relapsing pancreatitis). (abcam.com)
  • Lower than normal values in the blood and urine levels in a person showing symptoms of pancreatitis indicate a permanent damage in the amylase-producing cells of the pancreas. (medindia.net)
  • 2.2 Toxicological studies 2.2.1 Acute toxicity studies One study on the acute toxicity of maltogenic amylase has been reported as reported in Table 1. (inchem.org)
  • Pancreatic amylase occurs in serum at a concentration of about 60µg/L, from a total amylase concentration in serum of about 120µg/L. Pancreatic amylase and salivary amylase are the products of separate genes and, although they share 98% amino acid sequence homology, they differ in molecular size, isoelectric point and antigenic properties. (abcam.com)
  • This test measures the level of the enzyme amylase in the blood serum. (drmyhill.co.uk)
  • Four cases are reported of occupational asthma due to an enzyme (amylase), used in detergent washing powders. (ilo.org)
  • Hyperamylasemia may be described as an excess of the pancreatic enzyme - amylase in the blood. (chemocare.com)
  • The reason why foods that consist of complex starch, such as potato and rice, taste mildly sweet when chewed, is the action of the enzyme amylase. (medindia.net)
  • The normal amylase levels in the blood is 25 to 160 U/L (units per liter). (buzzle.com)
  • We also cover what the normal amylase range is and what high and low levels can mean. (medicalnewstoday.com)
  • Mid Temperature Alpha Amylase Enzyme Desizing Enzyme For Washing / Dyeing / Printing Mills Introduction: High concentrated thermo stable bacterial amylase which should be used as a formulating base for desizing fabrics, sized with starch, starch derivatives, or blends of starch. (splicd.com)
  • Medium Temp Alpha Amylase Enzyme for Pad Steam Desizing with Optimal Stength Retention Introduction: It is a concentrated thermo stable bacterial amylase which should be used as a formulating base for desizing fabrics, sized with. (splicd.com)
  • Most of your amylase is made in the pancreas and salivary glands. (medlineplus.gov)
  • In general, salivary amylase is more frequently glycosylated than pancreatic amylase, and it is still uncertain why differences in the glycosylation pattern among human amylase isozymes occur. (scirp.org)
  • Although there are two potential N-glycosylation sites (N427 and N476) in the C-terminal region of these amylases, glycosylation of the amylases seems to occur in a tissuespecific manner. (scirp.org)
  • It is still uncertain, however, why differences in the glycosylation pattern among human amylase isozymes occur. (scirp.org)
  • the β-amylases occur in higher plants. (diobeth.org)
  • You don't need any special preparations for an amylase blood or urine test. (medlineplus.gov)
  • Normally, only low levels of amylase are found in the blood or urine. (wellspan.org)
  • I was trying to find something about low amylase levels as mine were recently found to be. (medhelp.org)
  • While amylases are found naturally in yeast cells, it takes time for the yeast to produce enough of these enzymes to break down significant quantities of starch in the bread. (wikipedia.org)
  • This entry represents the all-beta C-terminal domain that is found in members of the glycosyl hydrolase 13 family, such as alpha-amylases and 1,4-alpha-glucan branching enzyme. (ebi.ac.uk)
  • Beta Amylase is an enzyme found in the seeds of higher plants and in sweet potatoes. (abcam.com)
  • Potential allergenicity of the β‐amylase was evaluated by searching for similarity of the amino acid sequence to those of known allergens, and no match was found. (europa.eu)
  • These amylases share a 97% - 99% amino acid sequence identity, and two potential N-glycosylation sites (N427 and N476) are commonly found in the C-terminal region. (scirp.org)
  • In this study, we found that there was no significant change of ratio of glycosylated molecules among isozymes produced by the same cultured cells, indicating that glycosylation of amylase is influenced by the type of cell producing the enzyme rather than being an inherent property of the amylase isozymes. (scirp.org)
  • We studied the structures of N-glycans of human pancreatic amylase and found that they are different from those of salivary amylase (Oyama et al. (scirp.org)
  • Research has found connections between low levels of salivary amylase and higher stress hormones in the body, so ensuring your count is correct means fewer mood swings and chronic stress. (organicfacts.net)
  • One or both types of tests may be used to help monitor amylase levels in people who are being treated for pancreatic or other disorders. (medlineplus.gov)
  • Elevated levels of amylase are usually indicative of medical conditions associated with the pancreas. (buzzle.com)
  • The following Buzzle write-up provides information on the possible contributing factors for elevated amylase levels. (buzzle.com)
  • This write-up provides information on the diagnostic test that is conducted for checking amylase levels. (buzzle.com)
  • Cancer of the pancreas, ovaries or lungs, pancreatic or bile duct blockage, gallbladder attacks caused by disease, intestinal blockages, perforated ulcers and tubal pregnancies may cause increased levels of amylase in the blood, states MedlinePlus. (reference.com)
  • Kidney disease, damage to the pancreas, cancer of the pancreas and toxemia of pregnancy are causes of low levels of amylase in the blood. (reference.com)
  • Industrial sectors with top-down and bottom-up approaches are currently focusing on improving microbial amylase production levels by implementing bioengineering technologies. (hindawi.com)
  • Alpha amylase levels in the blood are sometimes measured to diagnose diseases. (wisegeek.com)
  • A blood test may be done to check levels of amylase in the blood. (wisegeek.com)
  • Amylase levels may also be high in patients with inflammation or swelling of the gallbladder, obstruction of the bile duct, or peptic ulcers. (wisegeek.com)
  • Doctors can also use a urine test to check a person's amylase levels. (medicalnewstoday.com)
  • Two case studies suggest that there may be an association between high amylase levels in the blood and lung cancer . (medicalnewstoday.com)
  • In the blood, amylase levels rise for only a short time. (wellspan.org)
  • Many conditions can change amylase levels. (wellspan.org)
  • Tumors - Amylase enzyme levels may be increased in some pancreas, salivary, prostate, lung and ovarian tumors. (chemocare.com)
  • Gall bladder infection - Inflammation of the gall bladder (cholecystitis), may cause increased amylase levels , causing hyperamylasemia. (chemocare.com)
  • Certain medications too can increase amylase levels. (medindia.net)
  • If you have a pancreatic disorder, your amylase levels are usually higher than normal. (ahealthyme.com)
  • If your amylase levels are higher than normal, you may have one of many conditions. (ahealthyme.com)
  • Lynch, the principal investigator of the LIFE Study's psychological stress protocol, said that the study shows that women with high levels of the stress biomarker salivary alpha-amylase have a lower probability of becoming pregnant and it's associated with a greater than two-fold increased risk of infertility among these women. (thefreedictionary.com)
  • MINNEAPOLIS--Increased levels of the stress biomarker alpha-amylase , but not cortisol, are significantly associated with a lower chance of conception, a couple-based, prospective study has shown. (thefreedictionary.com)
  • What is Alpha Amylase? (wisegeek.com)
  • Alpha amylases are enzymes and, by definition, speed up the rate of reactions. (wisegeek.com)
  • This is initiated by chewing, and alpha amylase is one of the components of it. (wisegeek.com)
  • Pancreatic alpha amylase cleaves the linkages of amylose. (wisegeek.com)
  • Other organisms also produce alpha amylase. (wisegeek.com)
  • Alpha-amylase showed the highest toxicty in this category and thus read-across to alpha-amylase is considered to ensure safety for enzymes within this category, where no data are available. (europa.eu)
  • The worst case represented by alpha-amylases and thus data from amylases are judged to be representativ for non-proteolytic enzymes and read-across is fully applicable. (europa.eu)
  • Alpha-amylase is classified as family 13 of the glycosyl hydrolases and is present in archaea, bacteria, plants and animals. (ebi.ac.uk)
  • Alpha-amylase is an essential enzyme in alpha-glucan metabolism, acting to catalyse the hydrolysis of alpha-1,4-glucosidic bonds of glycogen, starch and related polysaccharides. (ebi.ac.uk)
  • Although all alpha-amylases possess the same catalytic function, they can vary with respect to sequence. (ebi.ac.uk)
  • Evolution of alpha-amylases: architectural features and key residues in the stabilization of the (beta/alpha)(8) scaffold. (ebi.ac.uk)
  • Cengiz S, Cavaz L, Yurdakoc K (2010) Alpha-amylase inhibition kinetics by caulerpenyne. (springer.com)
  • Your search returned 4 amylase, alpha 1B (salivary) ELISA ELISA Kit across 2 suppliers. (biocompare.com)
  • Alpha-amylase is widespread among living organisms. (diobeth.org)
  • In support of reservoir clean-up needs, Verenium's Eradicake™ alpha-amylase product addresses the removal of filter cake from the wellbore wall, a critical step in the drilling process. (synbioproject.org)
  • Laboratory and field tests have shown the effectiveness of Eradicake™ alpha-amylase in breaking down filter cake created by starch-based drilling muds. (synbioproject.org)
  • Using Eradicake™ alpha-amylase to break down the polymer structures of filter cake is easy and convenient (no specialized equipment is required), and safe for the operator and the environment. (synbioproject.org)
  • Eradicake™ alpha-amylase performs at higher temperatures and across a wider range of operating conditions than competitive products. (synbioproject.org)
  • Alpha-amylase functions as a salivary gland-specific self T cell epitope in patients with Sjögren's syndrome. (ingentaconnect.com)
  • One was a human alpha-amylase salivary precursor (AA54-407), suggesting that alpha-amylase might be a salivary gland-specific autoantigen. (ingentaconnect.com)
  • To examine whether alpha-amylase acts as an antigen in labial salivary glands, PBL from 11 patients with SS were incubated with 9 different synthetic amino acids of alpha-amylase or salivary alpha-amylase. (ingentaconnect.com)
  • These findings support the possibility that alpha-amylase functions as a salivary gland-specific T cell epitope and induces autoimmunity in SS. (ingentaconnect.com)
  • Liquozyme LpH is an alpha-amylase effective at low pH that thins the mash by breaking down starch into shorter dextrin chains. (biofuelsdigest.com)
  • Salivary alpha-amylase as a non-invasive biomarker for the sympathetic nervous system: current state of research. (thefreedictionary.com)
  • Verenium has granted DSM exclusive licenses to its alpha-amylase and xylanase enzyme products for use in the food and beverage markets. (thefreedictionary.com)
  • As part of the agreement Verenium has agreed to manufacture Purifine PLC, alpha-amylase and xylanase products for DSM. (thefreedictionary.com)
  • Verenium Corporation (Nasdaq: VRNM), an industrial biotechnology company focused on the development and commercialisation of high-performance enzymes, has launched VEREFLOW alpha-amylase , a unique enzyme for the removal of filter cake in drilling operations. (thefreedictionary.com)
  • There are various types of this enzyme, but all are digestive enzymes - alpha-amylase, beta-amylase, and y-amylase. (organicfacts.net)
  • C12N9/2414 - Alpha-amylase (3.2.1.1. (google.com)
  • There is provided a novel cold-active alpha-amylase identified by a functional metagenomic approach expressed in E. coli and purified to homogeneity. (google.com)
  • Functional, biochemical analysis has documented that the alpha-amylase is cold-adapted with a temperature optimum at 10 °C to 20 °C and that the enzyme is active over a broad pH range. (google.com)
  • Sequence analysis has indicated that the alpha-amylase is related to Clostridia , and has revealed classical characteristics of cold-adapted enzymes. (google.com)
  • Alpha-amylase is a starch hydrolyzing enzyme which has many industrial applications. (scirp.org)
  • Alpha-amylase represents about 30% of the whole enzyme market in the world. (scirp.org)
  • Question about Mash times, boil, alpha and beta amylase? (homebrewtalk.com)
  • Pancreatic Amylase Alpha Monoclonal antibody specifically detects Pancreatic Amylase Alpha in Rat samples. (fishersci.com)
  • And also, the rate of reaction between amylase and starch willbe affected because it may take more time to completely break down starch molecules if there ismore starch solution. (slideshare.net)
  • Amylase increases the rate of reaction by decreasing the activation energy needed to hydrolyse the starch molecules. (ipl.org)
  • Amylase breaks down starch into sugar, allowing large molecules to be digested easily. (ipl.org)
  • Effect of α-Amylase Concentration on the Rate of Starch Hydrolysis in a Porcine Pancreas Introduction: Our body uses various different types of molecules that work together in order to keep us functioning. (bartleby.com)
  • One of these molecules is called amylase. (bartleby.com)
  • In addition to the structural variety of N-glycans, there is a difference in the glycosylation pattern of human amylase isozymes [5,6]: both the N427 and N476 sites in human salivary amylase can be glycosylated to some extent, whereas those in human pancreatic amylase are either glycosylated to a lesser extent or not glycosylated. (scirp.org)