Ampholyte Mixtures

Isoelectric Focusing

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Chromatofocusing

List of MeSH codes (D27)

Solubility equilibrium

Immobilized pH gradient

Isoelectric focusing

Protein purification

Partition coefficient

Protein mixtures7

• Due to its high resolving power while retaining the native state, IEF is preferably used for the characterization of complex protein mixtures, particularly, if their components are almost indistinguishable, for example the isoforms of certain enzymes. (serva.de)
• Both, standard protein mixtures and also bacterial proteins were investigated. (mtak.hu)
• Martin RG, Ames BN (1961) A method for determining the sedimentation behavior of enzymes: application to protein mixtures. (springer.com)
• Whether used alone or as part of a broader separation or purification scheme, the MicroRotofor cell offers rapid, simple, and effective fractionation and enrichment of proteins from complex protein mixtures. (bio-rad.com)
• Two dimensional polyacrylamide gel electrophoresis (2-DE) is considered a powerful tool used for separation and fractionation of complex protein mixtures from tissues, cells, or other biological samples. (biomedcentral.com)
• 2-DE is a powerful and widely used method for analysis of complex protein mixtures with exceptional ability to separate thousands of proteins at once. (biomedcentral.com)
• This technique has become a routine method for DNA size fragmenting and separating protein mixtures in most laboratories around the world. (encyclopedia.pub)

Premixed with carrier ampholytes1

• Protein samples are first premixed with carrier ampholytes, additives and pI markers. (proteinsimple.com)

Isoelectric7

• Using this assembly, labelling of protein and peptide mixtures, their separation via free-flow isoelectric focusing and the determination of the isoelectric point (pI) of the separated products via the integrated sensor layer could be carried out within typically around 5 minutes. (rsc.org)
• c)-d) application of free-flow isoelectric focusing: c) a wide sample mixture and ampholytes are sandwiched between two thin sheath flow streams of high and low pH. (utwente.nl)
• Chromatofocusing is a protein-separation technique that allows resolution of single proteins and other ampholytes from a complex mixture according to differences in their isoelectric point. (wikipedia.org)
• MARSHALL, MAURICE R. 1990-04-01 00:00:00 ABSTRACT Isoelectric focusing (IEF) polyacrylamide gel containing an 80% pH 4-6.5 and 20% pH 3-10 ampholyte mixture greatly improved protein banding pattern for species identification of water extracts of raw pink, white and rock shrimp compared with the system using only the pH 3-10 range ampholyte. (deepdyve.com)
• This new method for fractionating alkaline phosphatase isoforms in hepatobiliary disorders is based on isoelectric focusing on a mixed-type polyacrylamide support containing an immobilized pH gradient with a superimposed carrier-ampholyte gradient. (elsevier.com)
• These isoforms focus in the pl 5-6 range (while alkaline phosphatases in normal serum focus in the pl 4-5 interval) in immobilized pH gradients, but behave as strongly acidic components by agarose isoelectric focusing in the presence of carrier ampholytes, suggesting that they are strongly complexed with the latter. (elsevier.com)
• however, some laboratories still use carrier ampholytes-based isoelectric focusing technique. (biomedcentral.com)

Buffers1

• The innovative format of this assay assures accurate quantitation from just 1 mL of sample in the presence of detergents, reducing agents and urea buffers containing up to 1% of carrier ampholytes. (thermofisher.com)

Gradient4

• d) In the presence of an electrical field the ampholytes build up and buffer a linear pH gradient. (utwente.nl)
• In routine analysis, precast gels with carrier ampholytes or with immobilized pH gradient are available to save time and effort and to provide reproducible, reliable results. (serva.de)
• Bio-Lyte ampholytes are required to form the internal pH gradient. (bio-rad.com)
• B)exhibits a stable pH gradient when ampholytes become distributed in an electric field. (quizplus.com)

Electrophoresis10

• A promising method in this respect is micro free-flow electrophoresis (μFFE) 2-4 because of its ability to continuously and preparatively separate and isolate biomolecules from mixtures on a small scale. (rsc.org)
• Illustration of the µ-FFE device and applied separation methods: a), b) application of free-flow zone electrophoresis: a) The sample mixture is hydrodynamically focused by two sheath flow streams containing buffer only and no voltage is applied. (utwente.nl)
• A mixture of 8 purified proteins and peptides (3.4 to 100kDa) for use as size standards in peptide and small protein gel electrophoresis. (fishersci.fi)
• A mixture of 11 proteins (5 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting. (fishersci.fi)
• A mixture of seven native proteins (14.4kDa to 116kDa) for use as size standards in protein electrophoresis (SDS-PAGE). (fishersci.fi)
• Mixture of 9 blue-, orange- and green-stained proteins (10 to 250kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting. (fishersci.fi)
• Electrophoresis is an analytical technique that has been applied to resolve complex mixtures containing DNA, proteins, and other chemical or biological species. (encyclopedia.pub)
• Microchip electrophoresis provides separations within a minute or few seconds while capillary electrophoresis takes from minutes to hours to fully resolve the components of the sample mixture. (encyclopedia.pub)
• Realities in resolution may be bad by perturbing immobilised pH centuries Sale filagra extra power 150 mg Anchorage the buffering species, which are involved to carrier ampholytes, Purchase Viagra Professional 100mg Portland copolymerised within the gel electrophoresis. (obyxova.ru)
• Professipnal titrate in the plane can be problematic and it is responsible to Online Perfopil Tablets Atlanta the ordinate, if relevant, in deionised hereditable or 2 per Purchase Viagra Professional 100mg Portland ampholytes, theorizing slippage or gel electrophoresis if continuous. (obyxova.ru)

Compounds1

• For example, in the manufacture of synthetic drugs, mixtures containing variable proportions of several compounds usually arise. (enacademic.com)

Peptides4

• The merits of hydrophobic interaction chromatography on octyl-Sepharose as a method for the separation of carrier ampholytes from peptides have been investigated. (uu.nl)
• A standard mixture of nine peptides containing approximately 550 pmol of total phosphopeptide was incubated with 400 mg of Captivate ferrofluid phosphopeptide-binding reagent. (thermofisher.com)
• Snake venom is a complex mixture of proteins and peptides, and a number of studies have described the biological properties of several venomous proteins. (biology-online.org)
• In contrast, Chp F formed short assemblies containing a mixture of random coil and β-sheet structure at a basic pH of 10.0, where only 40% of the peptides converted to fibrils. (mdpi.com)

Glycine2

• It is the purpose of this and the following paper to test portions of the theory in a more quantitative manner, and in this paper the mutual action of two ampholytes, glycine and sulfanilic acid, is studied by means of titration over a wide pH range. (caltech.edu)
• GE Healthcare Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals. (fishersci.co.uk)

Cationic1

• Here, poly(ampholytes), which contain a mixture of cationic and anionic side chains are quantitatively evaluated for their IRI activity. (rsc.org)

Polymers1

• According to the patent, the instant ampholyte polymers and polymer compositions condition and improve the skin and nails. (cosmeticsandtoiletries.com)

Buffer2

• After vacuum drying, Protein was extracted by vortexing 100 mg of seed powder with 1.5 ml of extraction buffer containing CHAPS (4%), urea (5 M), thiourea (2 M), (0.8%) ampholytes [pH 3 - 10] and DTT (65 mM) for 5 min at room temperature. (scirp.org)
• Proteins of the crushed seeds were precipitated for 2 hours 30 min at −20˚C in an acetone/TCA buffer containing 10% of TCA, 0.07% of 2-mercaptoethanol and acetone, and the mixture was centrifuged at 4000 rpm for 1 hour at −20˚C. Then, the pellet was rinsed twice at −20˚C for 1 hour in 2-mercaptoethanol and acetone buffer and centrifuged as previously done. (scirp.org)

Substances6

• The mixture of isoproturon and metazachlor tested on S. marinoi caused a 28-34% decrease in ϕ' that was significantly higher than levels induced by each of substances when tested alone. (bioportfolio.com)
• This strong decrease in ϕ' could be due to a combined effect of these substances on the photosynthetic apparatus, which is likely the cause of the synergy found for this mixture. (bioportfolio.com)
• The mixture of substances (proteins) to be fractionated is dissolved in a liquid or gaseous fluid called the mobile phase. (slideplayer.com)
• Separation methods, then, can be defined as processes that change the relative amounts of substances in a mixture. (enacademic.com)
• First, the mixture may contain some substance that should be isolated from the rest of the mixture: this process of isolating and thus removing substances considered to be contaminants is called purification. (enacademic.com)
• Convert units - Convert mol in quantity - Convert quantity in mol - Convert mass constituent in molarity - Convert molarity in mass constituent - Determine molarity - Determine molality - Solution of crystalline water substances - Mixture rules / cross - Solution of titrimetric standard substances 3. (lcs-chemie.de)

Sodium1

• Further, the aqueous gel medium comprises at least one ingredient selected from the group consisting of: carbomers, polymeric emulsifying agents, polysaccharidic biopolymers, gums, alginates, modified celluloses, starch derived products, polysorbate 80 (and) isohexadecane (and) acrylamide/sodium acryloyldimethyltaurate, and mixtures thereof. (cosmeticsandtoiletries.com)

Complex1

• Proteomics now makes it possible to globally identify proteins from a complex mixture. (biology-online.org)

Separation1

• A separation of a somatomedin-containing peptide mixture into seven fractions by hydrophobic interaction chromatography on octyl-Sepharose is shown. (uu.nl)

Gradients1

• A mixture of human serum albumin (pI 4.6) and beta-lactoglobulin (pI 5.22) was separated in pH gradients, generated with carrier ampholytes of different origin and covering the pH ranges 4-6.5, 3.5-5, 4-5.5 and 4.5-5.0. (courtfield.ml)

Vacuum1

• 5 times of this solution, add the activity of ampholytes specified in the vacuum and Purchsae to 10 years with reference R. Mix officially and transfer the disease. (obyxova.ru)

Samples1

• Identification of a specific species in mixture samples was achieved by the detection of water‐extractable shrimp specific protein bands present in the gel. (deepdyve.com)

Marker1

• Quite common is the pI determination via coelectrophoresis of known protein marker mixtures. (serva.de)

Identification1

• This fatty acid reference mixture contains saturated and mono and polyunsaturated fatty acid methyl esters for the qualitative identification and quantitation of unknowns. (universalbiologicals.com)

Capillary1

• The sample mixture is injected to fill the entire capillary column. (proteinsimple.com)

Species3

• Significant cases of antagonism (for both species) and synergism (for S. marinoi) were observed for the mixtures of isoproturon and spiroxamine, and isoproturon and metazachlor, respectively. (bioportfolio.com)
• These two mixtures, together with that of isoproturon and diuron, for which additivity was observed, were further studied for their impacts on the physiology of each species. (bioportfolio.com)
• Isoproturon and diuron singly and in mixtures induced 20-40% decreases in ϕ' which was in turn responsible for a significant decrease in relative lipid content for both species. (bioportfolio.com)

Temperature1

• Daily temperature variation magnifies the toxicity of a mixture consisting of a chemical pesticide and a biopesticide in a vector mosquito. (bioportfolio.com)

Sample2

• The sample mixture is injected into the carrier electrolyte flow and with increasing residence time the differently charged components split up into diverging lanes which can be collected at various device outlets, as shown in Fig. 1 . (utwente.nl)
• A poly(octadecyl acrylate-co-ethylene dimethacrylate) monolithic column served as the trap column to capture sample and to remove the ampholytes from CIEF. (dicp.ac.cn)

Chemical1

• The contaminants, instead of single contaminants, are usually found as chemical mixtures in terrestrial environment. (bioportfolio.com)

Detection1

• The proteins were then measured with the Maurice imaged CIEF system (native fluorescence detection), testing various measurement conditions, such as different ampholytes or ampholyte mixtures. (bvsalud.org)

Behavior1

• The University of Michigan Medical School, Ann Arbor), and Jane E. Experimental behavior is reproduced by computer simulation of a model mixture of 15 hypothetical carrier ampholytes whose pIs span the pH range 3-10. (wearburn.info)

Analysis2

• We tried the application of this method for the analysis of the ampholytes, which would be the most important question when the two technique is coupled sequentially. (mtak.hu)
• By studying problems with the quantitative analysis of animal and vegetable oils and fats, the American Oil Chemists' Society has found certain mixtures to be useful as reference standards. (universalbiologicals.com)

Components1

• The removal of the desired drug from the rest of the mixture is important if the product is to have uniform potency and is to be free of other components that may be dangerous to the body. (enacademic.com)

Resolve1

• 02 pH tings may be very riding a Profesaional prepared with carrier ampholytes while immobilised pH widths can resolve proteins differing by approximately 0. (obyxova.ru)

Standard1

• This AOCS RM mixture is especially ideal as a standard for linseed, perilla, hempseed, and rubberseed oils. (universalbiologicals.com)

Solution1

• 40. 5 volumes of this solution, add the mixture Hindgra sildenafil citrate ampholytes specified Hindgra sildenafil citrate the monograph and dilute to 10 volumes with water R. (shaymuratovo.ru)

Study1

• This study screened binary mixtures of pesticides for potential synergistic interaction effects on growth of the marine microalgae Tisochrysis lutea and Skeletonema marinoi. (bioportfolio.com)

Activity1

• The charge balance of the side chains is shown to be crucial, with only 50 : 50 mixtures having strong IRI activity, which also scales with molecular weight. (rsc.org)

Highly1

• Calibrate LTQ Velos, LTQ Orbitrap and other mass spectrometers using a mixture of highly purified ionizable molecules designed for positive mode calibration. (fishersci.co.uk)

Growth1

• Exposures were thus made in culture flasks at three concentrations, or concentration combinations for mixtures, selected to cause 25%, 50% and 75% growth rate inhibition. (bioportfolio.com)

Bands1

• Interferometer between water bands on an IEF gel prepared with Professilnal ampholytes can be efficiently good. (obyxova.ru)

Column1

• The protein mixture is bound to the topmost portionof the ion exchanger in the chromatography column. (slideplayer.com)

Size1

• Working with proteins In a mixture of the five proteins listed below,which should elute second in size-exclusion (gel- filtration)chromatography? (quizplus.com)