Ampholyte Mixtures: Such mixtures of amphoteric electrolytes or buffers that provide a continuous range of pH in an electric field; used for separating proteins by their isoelectric points, i.e., by isoelectric focusing.Isoelectric Focusing: Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point.Dictionaries, MedicalDictionaries as Topic: Lists of words, usually in alphabetical order, giving information about form, pronunciation, etymology, grammar, and meaning.Dictionaries, ChemicalElectrophoresis: An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current.Microfluidic Analytical Techniques: Methods utilizing the principles of MICROFLUIDICS for sample handling, reagent mixing, and separation and detection of specific components in fluids.Miniaturization: The design or construction of objects greatly reduced in scale.Cell Fractionation: Techniques to partition various components of the cell into SUBCELLULAR FRACTIONS.Electrophoresis, Microchip: A highly miniaturized version of ELECTROPHORESIS performed in a microfluidic device.Microfluidics: The study of fluid channels and chambers of tiny dimensions of tens to hundreds of micrometers and volumes of nanoliters or picoliters. This is of interest in biological MICROCIRCULATION and used in MICROCHEMISTRY and INVESTIGATIVE TECHNIQUES.Proteomics: The systematic study of the complete complement of proteins (PROTEOME) of organisms.Decapoda (Crustacea): The largest order of CRUSTACEA, comprising over 10,000 species. They are characterized by three pairs of thoracic appendages modified as maxillipeds, and five pairs of thoracic legs. The order includes the familiar shrimps, crayfish (ASTACOIDEA), true crabs (BRACHYURA), and lobsters (NEPHROPIDAE and PALINURIDAE), among others.Urea: A compound formed in the liver from ammonia produced by the deamination of amino acids. It is the principal end product of protein catabolism and constitutes about one half of the total urinary solids.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Penaeidae: A family of CRUSTACEA, order DECAPODA, comprising the penaeid shrimp. Species of the genus Penaeus are the most important commercial shrimp throughout the world.DNA Barcoding, Taxonomic: Techniques for standardizing and expediting taxonomic identification or classification of organisms that are based on deciphering the sequence of one or a few regions of DNA known as the "DNA barcode".Molecular Weight: The sum of the weight of all the atoms in a molecule.Gels: Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid phase liquefies; the resulting colloid is called a sol.Saint Vincent and the Grenadines: A self-governing state of the Windward Islands in the West Indies, comprising Saint Vincent and the northern islets of the Grenadines. Its capital is Kingstown. It is one of the original homes of the Carib Indians supposed to have been sighted by Columbus in 1498. It was in English hands from 1627 till held by the French 1779-83. Saint Vincent subsequently became a British possession and, with other nearby British territories, was administered by the Governor of the Windward Islands till 1959. It attained a measure of independence in 1969 but achieved full independence as Saint Vincent and the Grenadines in 1979. Saint Vincent was the 4th century Spanish martyr on whose feast day Columbus discovered the island. Grenadines is derived from the Spanish kingdom of Granada. (From Webster's New Geographical Dictionary, 1988, p1054 & The Europa World Year Book 1993, p2441)Spectrum Analysis, Raman: Analysis of the intensity of Raman scattering of monochromatic light as a function of frequency of the scattered light.Sorbose: A ketose sugar that is commonly used in the commercial synthesis of ASCORBIC ACID.Disclosure: Revealing of information, by oral or written communication.Magnetic Resonance Spectroscopy: Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).Truth Disclosure: Truthful revelation of information, specifically when the information disclosed is likely to be psychologically painful ("bad news") to the recipient (e.g., revelation to a patient or a patient's family of the patient's DIAGNOSIS or PROGNOSIS) or embarrassing to the teller (e.g., revelation of medical errors).Indicators and Reagents: Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)PaperIllusions: The misinterpretation of a real external, sensory experience.Patents as Topic: Exclusive legal rights or privileges applied to inventions, plants, etc.Immunoassay: A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.Immunochromatography: A type of affinity chromatography where ANTIBODIES are used in the affinity capture reaction on the solid support, in the mobile phase, or both.Reagent Strips: Narrow pieces of material impregnated or covered with a substance used to produce a chemical reaction. The strips are used in detecting, measuring, producing, etc., other substances. (From Dorland, 28th ed)Sensitivity and Specificity: Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)Apolipoprotein A-I: The most abundant protein component of HIGH DENSITY LIPOPROTEINS or HDL. This protein serves as an acceptor for CHOLESTEROL released from cells thus promoting efflux of cholesterol to HDL then to the LIVER for excretion from the body (reverse cholesterol transport). It also acts as a cofactor for LECITHIN CHOLESTEROL ACYLTRANSFERASE that forms CHOLESTEROL ESTERS on the HDL particles. Mutations of this gene APOA1 cause HDL deficiency, such as in FAMILIAL ALPHA LIPOPROTEIN DEFICIENCY DISEASE and in some patients with TANGIER DISEASE.Cholesterol, HDL: Cholesterol which is contained in or bound to high-density lipoproteins (HDL), including CHOLESTEROL ESTERS and free cholesterol.Point Mutation: A mutation caused by the substitution of one nucleotide for another. This results in the DNA molecule having a change in a single base pair.Hypolipoproteinemias: Conditions with abnormally low levels of LIPOPROTEINS in the blood. This may involve any of the lipoprotein subclasses, including ALPHA-LIPOPROTEINS (high-density lipoproteins); BETA-LIPOPROTEINS (low-density lipoproteins); and PREBETA-LIPOPROTEINS (very-low-density lipoproteins).Pedigree: The record of descent or ancestry, particularly of a particular condition or trait, indicating individual family members, their relationships, and their status with respect to the trait or condition.Lipids: A generic term for fats and lipoids, the alcohol-ether-soluble constituents of protoplasm, which are insoluble in water. They comprise the fats, fatty oils, essential oils, waxes, phospholipids, glycolipids, sulfolipids, aminolipids, chromolipids (lipochromes), and fatty acids. (Grant & Hackh's Chemical Dictionary, 5th ed)Polymorphism, Restriction Fragment Length: Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment.Inventions: A novel composition, device, or process, independently conceived de novo or derived from a pre-existing model.Materials Testing: The testing of materials and devices, especially those used for PROSTHESES AND IMPLANTS; SUTURES; TISSUE ADHESIVES; etc., for hardness, strength, durability, safety, efficacy, and biocompatibility.Biotechnology: Body of knowledge related to the use of organisms, cells or cell-derived constituents for the purpose of developing products which are technically, scientifically and clinically useful. Alteration of biologic function at the molecular level (i.e., GENETIC ENGINEERING) is a central focus; laboratory methods used include TRANSFECTION and CLONING technologies, sequence and structure analysis algorithms, computer databases, and gene and protein structure function analysis and prediction.Filtration: A process of separating particulate matter from a fluid, such as air or a liquid, by passing the fluid carrier through a medium that will not pass the particulates. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Buffers: A chemical system that functions to control the levels of specific ions in solution. When the level of hydrogen ion in solution is controlled the system is called a pH buffer.Micropore Filters: A membrane or barrier with micrometer sized pores used for separation purification processes.Anion Exchange Resins: High-molecular-weight insoluble polymers that contain functional cationic groups capable of undergoing exchange reactions with anions.Compressive Strength: The maximum compression a material can withstand without failure. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed, p427)Chromatography, High Pressure Liquid: Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.Chromatography, Ion Exchange: Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins.Chromatography, Affinity: A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Chromatography, Gel: Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.

Adenosine-5'-O-phosphorylated and adenosine-5'-O-phosphorothioylated polyols as strong inhibitors of (symmetrical) and (asymmetrical) dinucleoside tetraphosphatases. (1/14)

Dinucleoside 5',5"'- P (1), P ( n )-polyphosphates, and particularly the diadenosine compounds, have been implicated in extracellular purinergic signalling and in various intracellular processes, including DNA metabolism, tumour suppression and stress responses. If permitted to accumulate, they may also be toxic. One approach to understanding their function is through the various specific degradative enzymes that regulate their levels. Eight adenosine-5'- O -phosphorylated polyols (derivatives of glycerol, erythritol and pentaerythritol) and 11 adenosine-5'- O -phosphorothioylated polyols (derivatives of glycerol, erythritol, pentaerythritol, butanediol and pentanediol) have been tested as inhibitors of specific diadenosine tetraphosphate (Ap(4)A) hydrolases. Of these two groups of novel nucleotides, the adenosine-5'- O -phosphorothioylated polyols were generally stronger inhibitors than their adenosine-5'- O -phosphorylated counterparts. 1,4-Di(adenosine-5'- O -phosphorothio) erythritol appeared to be the strongest inhibitor of ( asymmetrical ) Ap(4)A hydrolases (EC 3.6.1.17) from both lupin and human, with K (i) values of 0.15 microM and 1.5 microM respectively. Of eight adenosine-5'- O -phosphorylated polyols, 1,4-di(adenosine-5'- O -phospho) erythritol was the only compound that inhibited the lupin enzyme. Two derivatives of pentaerythritol, di(adenosine-5'- O -phosphorothio)-di(phosphorothio) pentaerythritol and tri(adenosine-5'- O -phosphorothio)-phosphorothio-pentaerythritol, proved to be the strongest inhibitors of the prokaryotic ( symmetrical ) Ap(4)A hydrolase (EC 3.6.1.41) so far reported. The estimated K (i) values were 0.04 microM and 0.08 microM respectively. All of these inhibitors were competitive with respect to Ap(4)A. These new selectively acting Ap(4)A analogues should prove to be valuable tools for further studies of Ap(4)A function and of the enzymes involved in its metabolism.  (+info)

Efficient solubilization buffers for two-dimensional gel electrophoresis of acidic and basic proteins extracted from wheat seeds. (2/14)

Plant tissues are made up of a broad range of proteins with a variety of properties. After extraction, solubilization of a diverse range of plant proteins for efficient proteomic analysis using two-dimensional electrophoresis is a challenging process. We tested the efficiency of 12 solubilization buffers in dissolving acidic and basic proteins extracted from mature seeds of wheat. The buffer containing two chaotropes (urea and thiourea), two detergents (3-[(3-cholamidopropyl) dimethyl-ammonio]-1-propane-sulfonate and N-decyl-N,N-dimethyl-3-ammonio-1-propane-sulfonate), two reducing agents (dithiothreitol and tris (2-carboxyethyl) phosphine hydrochloride) and two types of carrier ampholytes (BioLyte pH 4-6 and pH 3-10) solubilized the most acidic proteins in the pH range between 4 and 7. The buffer made up of urea, thiourea, 3-[(3-cholamidopropyl) dimethyl-ammonio]-1-propane-sulfonate, DeStreak reagent (Amersham Biosciences, Uppsala, Sweden) and immobilized pH gradient buffer, pH 6-11 (Amersham Biosciences) solubilized the most basic proteins in the pH range between 6 and 11. These two buffers produced two-dimensional gels with high resolution, superior quality and maximum number of detectable protein (1425 acidic protein and 897 basic protein) spots.  (+info)

Commercial ampholytes used for isoelectric focusing may interfere with bioactivity based purification of antimicrobial peptides. (3/14)

BioRad's Rotofor system has been frequently used for the purification of proteins and smaller peptides such as bacteriocins. In this study, we report that some commercially available ampholytes used with the Rotofor isoelectric focusing system possess antimicrobial activity, which may interfere with the purification of bacteriocins and bacteriocin-like substances.  (+info)

Infection with Panton-Valentine leukocidin-positive methicillin-resistant Staphylococcus aureus t034. (4/14)

 (+info)

Effect of synthetic carrier ampholytes on saturation of human serum transferrin. (5/14)

We have investigated the effect in solution of synthetic carrier ampholytes on the saturation of human serum transferrin. By spectrophotometric titrations of human serum transferrin with various Fe3+-carrier ampholyte solutions, we demonstrated that under these conditions carrier ampholytes behave as typical chelators, their binding curves being very similar to that obtained with disodium nitrilotriacetate. On performing titration experiments at three different pH values, carrier ampholytes act like nitrilotriacetate at pH 7.5, but the former are more effective iron donors at pH 8.4 and worse iron donors at pH 5.2. Spectrophotometric titrations of isolated C-terminal and N-terminal fragments obtained from human serum transferrin by thermolysin cleavage show no differences between them, and no differences with respect to the whole protein except that they contain half the number of binding sites. In order to determine a site-specificity of iron in the presence of ampholytes, the classical urea/polyacrylamide-gel-electrophoresis technique was adopted. Under saturating conditions carrier ampholyte solutions act mostly on the C-terminal site, whereas desaturating agents remove iron preferentially from the N-terminal site. Our findings support the hypothesis that Ampholine may chelate Fe3+ as well as many other compounds.  (+info)

Resolution of alkaline phosphatase isoenzymes in serum by isoelectric focusing in immobilized pH gradients. (6/14)

This new method for fractionation of serum alkaline phosphatase isoenzymes is based on isoelectric focusing on a mixed-type polyacrylamide support containing an immobilized pH gradient with a superimposed carrier-ampholyte gradient. All known forms of alkaline phosphatase are separated in an Immobiline pH 3.5-6.0 gradient, the sample being applied into pockets cast on a pH 8.0 plateau. Sharp zymogram bands are obtained by substituting alkaline-stable 5-bromo-4-chloro-3-indoxyl phosphate and tetrazolium salts for the standard 1- and 2-naphthyl phosphate-diazonium salt combinations. After hydrolysis of the phosphate group by the alkaline phosphatase the indoxyl moieties reduce tetrazolium salts to nearly insoluble and nondiffusible formazan precipitates. Normal sera show an array of about 10 isobands isoelectric between pH 3.9 and pH 4.79. In Paget's disease, two sharp isobands with pls of 4.97 and 5.09 are seen. Placental alkaline phosphatase overlaps with the higher pl bands of normal serum; however, upon heat destruction of the latter, it shows four sharp bands with the following pl's: 4.59, 4.62, 4.67 and 4.73.  (+info)

Charge microheterogeneity of the major capsid protein of polyoma virus. (7/14)

The behavior in isoelectric focusing of the major capsid polypeptide VPI of several strains of polyoma virus was studied. Two previously recognized phenomena were reexamined, namely, (i) the separation of the VP1 polypeptide into multiple subspecies differing only slightly from each other in apparent isoelectric point and (ii) strain differences in the overall apparent net charge of the family of VP1 subspecies. It was found that the pattern of subspecies was reproducible when focusing was initiated from either the basic or acidic region of the gel, keeping the ampholyte mixture constant. However, individual subspecies were unstable, and labeled polypeptide could be shifted dramatically by either refocusing of separated subspecies or by altering the concentration of ampholytes. These findings suggest that protein-protein and protein-ampholyte interactions play an important role in the generation of this charge heterogeneity. The basis for the overall charge difference between the VP1 of 3049 virus and several other strains (lpD, lpS, ts59, and A2) was studied, using recombinant viruses constructed of specific sequences derived from 3049 and lpD genomes. The portion of the VP1 polypeptide carrying the altered charge could be mapped to the body of the molecule 3' to the HindIII site at 45.0 map units (3,918 base pairs). This clearly segregates the VP1 charge phenotype from the cyc phenotype of 3049 in which capsid proteins are overproduced and accumulate in the cytoplasm of infected cells.  (+info)

pH-dependent aggregation and electrofocusing of poliovirus. (8/14)

Following isoelectric focusing, poliovirus can be detected at two pH values. The acidic form consists of poliovirus aggregates and the neutral form of single virions.  (+info)

Browse our electrophoresis products: carrier ampholytes, electrophoresis chamber, power supply, acrylamide gel support sheets and seed purity testing kits.
* found in: Pharmalyte, Pharmalyte, narrow range pH 4.2-4.9 Pharmalyte, narrow range pH 4.2-4.9 Pharmalyte carrier ampholytes, prepared by the co..
Method of producing electrical layer capacitors with glow-polymerizate layers as dielectrics, including a support surface, which includes initially forming a synthetic carrier strip having a substantially rectangular cross-section as well as given parts not to be coated with glow-polymerizate and a surface to be covered with glow-polymerizate, applying metal coating layers and glow-polymerizate layers offset from each other on the carrier strip forming contact strips on two mutually opposing edges of the carrier strip, the contact strips having within the extent thereof at least two metal layers lying directly on each other, and covering the given parts of the carrier strip not to be coated with at least one screen during the manufacture of the glow-polymerizate layers, wherein the improvement includes forming the recesses on areas of the carrier strip bordering the contact strips and not coated with glow-polymerizate, pressing the carrier strip against the support surface with holding devices
Isoelectric Focusing (IEF) can be described as an ingenious process for simultaneous concentration and separation of proteins. IEF employs a pH gradient formed by small amphoteric molecules (called ampholytes) to resolve proteins according to their different pI values (pI = isoelectric point). IEF is an end point method - when the electrophoretic run is completed proteins will appear as separate, sharp zones, in the order of their isoelectric points.. For IEF, SERVA offers a comprehensive product line: ...
Browse Sigma-Aldrichs Peptide Analysis and Characterization to find products in Amino Acid Analysis, Ampholytes, Crystallization Reagents, Crystallization and Renaturation Screening Kit Components
Learn more about adenosine-5-diphosphate-disodium-salt-adp-disodium-salt. We enable science by offering product choice, services, process excellence and our people make it happen.
Learn more about adenosine-5-o-3-thiotriphosphate-tetralithium-salt. We enable science by offering product choice, services, process excellence and our people make it happen.
Brown, R.K., Caspers, M.L., Lull, J.M., Vinogradov, S.N., Felgenhauer, K. and Nekic, M. (1977). Carrier Ampholyte Distribution in Isoelectric Focusing. J. Chromatogr. 131, 223-232.. Brown, R.K., Caspers, M.L. and Vinogradov, S.N. (1977). Carrier Ampholyte Distribution. In Electrofocusing and Isotachophoresis (Radola, B.J. and Graesslin, D. Eds.) Walter DeGrutyer and Co., Berlin-New York, pp 87-96.. Caspers, M.L., Posey, Y. and Brown, R.K. (1977). Separator Isoelectric Focusing - An Improved Method of Protein Analysis and Purification. Anal. Biochem. 79, 166-180.. Caspers, M.L. and Chrambach, A. (1977). Natural pH Gradients Formed by Amino Acids: Ampholyte Distribution, Time Course, Use in Electrofocusing of Protein, Relation to pH Gradients in Isotachophoresis, Separator Effects. Anal. Biochem. 81, 23-39.. Caspers, M.L. and Siegel, G.J. (1980). Inhibition by Lead of Human Erythrocyte (Na+ + K+)-Adenosine Triphosphatase Associated with Binding of 210Pb to Membrane Fragments. Biochim. et. Biophy. ...
Such mixtures of amphoteric Electrolytes or Buffers that provide a continuous range of pH in an electric field; used for separating Proteins by their Isoelectric Points, i.e., by Isoelectric Focusing ...
Introduction: The whole delivery process of nucleic acids is very challenging. Appropriate carrier systems are needed, which show extracellular stability and intracellular disassembly. Viruses have developed various strategies to meet these requirements, as they are optimized by biological evolution to transfer genetic information into host cells. Taking viruses as models, smart synthetic carriers can be designed, mimicking the efficient delivery process of viral infection. These synthetic viruses are pre-programmed and respond to little differences in their microenvironment, caused by either exogenous or endogenous stimuli. Areas covered: This review deals with polymer-based, bioresponsive nanosystems (polyplexes) for the delivery of nucleic acids. Strategies utilizing pH-responsiveness, redox-responsiveness as well as sensitivity towards enzymes will be described more in detail. Systems, which respond to other endogenous triggers (i.e. reactive oxygen species, adenosine triphosphate, ...
Among the key variations between conventional skin care and natural or organic skin care isnt the "active" ingredients for example eco-friendly tea or vitamin c, which excluding water could make as much as about 5% associated with a product, rather it lies using the base ingredients. In natural skin care, the bottom ingredients are frequently a mixture of vegetable oils and butters or waxes as opposed to the synthetic ingredients frequently present in conventional skin care. Using base oils has enormous benefit for that skin. Rather to be an inert (non-active) synthetic carrier for that ingredients, base oils contain nutrients for example vitamins, minerals and efas that support and nourish the skin. Id go to date regarding count base oils as ingredients in skin care. So, actually, in natural products as much as 95% associated with a product includes a supportive "active" impact on the skin. Compared, the synthetic base ingredients in conventional skin care by enlarge, lack significant ...
Goal of the project is to design novel approaches strengthening tolerance towards autoantigens by induction of regulatory T cells (Tregs). For this, modifications of autoantigen-derived peptides are being developed: Peptides coupled to either synthetic carriers or to ligands triggering tolerogenic responses are tested in vitro and in autoimmune models. In addition, the project searches for novel drug candidates able to induce stable Tregs. To improve efficacy of tolerogenic treatments in established disease, combination therapies deleting/inactivating effector cells followed by tolerogenic vaccination will be applied. ...
Craig Duvall, associate professor of biomedical engineering, put the effectiveness of a specialized ribonucleic acid hitchhiking on the human protein albumin up against jetPEI nanoparticles, the mostly widely used synthetic carrier for the task of tumor gene silencing.. His findings, reached with Samantha Sarett, a recent biomedical engineering Ph.D. graduate, are published today(Monday, July 24) in the Proceedings of the National Academy of Sciences.. Albumin is Trojan horse. Ribonucleic acids can control the behavior of cancer cells, but they require a carrier to get them to the target. Duvalls team made a simple modification to a small-interfering ribonucleic acid molecule, called siRNA-L2, allowing it to rapidly load into an albumin pocket typically reserved to ferry fatty acids around the body.. They found that the siRNA-L2, using albumin as its carrier, has no apparent dose-limiting toxicity, a significant problem for synthetic nanoparticles. That means a higher dose of the anti-cancer ...
Charge-Exchange Plasma Contamination on SMART-1: First Measurements and Model Verification Publication date: 12 July 2004 Authors: Tajmar, M., et al. Year: 2004 .... description
At least 75 percent of all hydraulic systems fail due to contaminated or aging hydraulic fluid.1 Contamination causes aging/degradation of fluids and hydraulic systems failure for numerous...
It is impossible to disinfect an inadequately cleaned instruments. Check out our solutions to detect biofilms on surgical instrumentation.
Looking for RPI AMP (Adenosine-5-monophosphate),5g (31FV79)? Graingers got your back. Price:$40.70. Easy ordering & convenient delivery. Log-in or register for your pricing.
Another option is to simulate fit and unfit. To do this the central bank gathers data from at least 10,000 circulated banknotes, and the tool uses this data to calculate the unfit rate based on current sorting thresholds. The customer is then able to explore various threshold scenarios for which the BPS® Eco-Comparator simulates the new unfit rate: "How many banknotes would be destroyed if less contamination on the front were acceptable?" This enables the central bank to model how many banknotes it wants to replace.. Where adjusting sorting thresholds used to be a matter of gut instinct, the BPS® Eco-Comparator is a decisive step towards fact-based decision-making. And its a methodology that pays off quickly too: If the cost of producing a banknote is €0.10, and the processing volume of a BPS® is around 500,000 banknotes per day, 1% less shredding results in savings of around €100,000 per machine per year.. ...
We,China Adenosine-5-monophosphate 61-19-8 Suppliers and China Adenosine-5-monophosphate 61-19-8 Manufacturers, provide Adenosine-5-monophosphate 61-19-8 product and the products related with China Adenosine-5-monophosphate 61-19-8 - chengyipharma
An interesting question and one that long needs answering. Thus far I have found a dearth of negative research on the topic. In 2005 the American Meat Inst
Presented by Alex Henri MD How prone are UAs to contamination, can we identify contamination on a UA, and does contamination matter? How common is contamination?
Targets Escherichia coli O157:H7 contamination on hides of live animals. For product inquires or to place an order, email Intralytix at [email protected] or call 1-877-ITXPHAGE (489-7424) and select option 1. ...
The chromatographic behaviour of recombinant human tumour necrosis factor beta (rhTNF-β) (pI ~9.0) during cation-exchange chromatography at pH 7.5 is investigated. Without prior treatment of the Escherichia coli cell extract with polyethyleneimine (PEI), very little rhTNF-β was bound to the column. However, upon addition of 5% PEI (100 μl ml-1) to the cell lysate, rhTNF-β was shown to bind to cation-exchange columns normally. TNF-β was readily precipitated from the clarified cell extract by 20% ammonium sulphate, but only ca. 25% of this precipitate could be re-solubilized for further purification. However, when 5% PEI was included in the solubilization buffer, the balance of the rhTNF-β could be recovered. It is proposed that charge interaction between rhTNF-β and nucleic acids in the cell extract is responsible for both of these anomalous phenomena, and that PEI (a cationic polyelectrolyte) was able to disrupt this interaction by displacing rhTNF-β from the charge complex ...
1B3E: X-ray crystallography and mass spectroscopy reveal that the N-lobe of human transferrin expressed in Pichia pastoris is folded correctly but is glycosylated on serine-32.
In this work, we present a step‐by‐step workflow for the fabrication of 2D hexagonal boron nitride (h‐BN) nanopores which are then used to sense holo‐human serum transferrin (hSTf) protein at pH ∼8 under applied voltages ranging from +100 mV to +800 mV. 2D nanopores are often used for DNA, however, there is a great void in the literature for single‐molecule protein sensing and this, to the best of ...
Finding mice or rodents inside your home can really be disturbing. Most of us will feel repulsed just by the sight of them. Having a single rodent inside a home can be dangerous because they have the ability to produce more than 60,000 droppings and 150 liters of urine. Imagine the risk of contamination by a single rodent, especially when it is not eliminated right away. The presence of rodents can bring about a spread of disease, contamination on foods and produce, and the damage to structures and equipment.. It is not safe to have these rodents in and around the house since they have the ability to feed on different types of foods while carrying different infectious diseases. One of the most dangerous diseases these rodents can pass is leptospirosis, which can be spread through their urine. Rodents also have the ability to spread and populate in just a matter of weeks. With the different complications these pests can bring, homeowners are always suggested to be cautious against them, and ...
Adenosine-5-triphosphate - adenozynotrifosforan (ATP), nukleotyd adenionowy (składa się z adenozyny i trzech grup fosforanowych. ATP jest wielofunkcyjnym nu...
Daniel called the police so that we could file a report and I called my OB to see if we should be concerned. Thankfully the lap part of my seat belt did not tighten and the chest part compressed mostly to the left and above my belly. I was feeling fine with just a bit of tenderness where the seat belt had tightened, so my doctor and I agreed that I didnt need to go to the hospital. HOWEVER if I started to experience contractions or bleeding, I needed to call her immediately. Our other concern was that if I did have any problems, I was about an hour from the hospital - not really enough time for any type of worse-case scenario. ...
The complexation of Cm(iii) with human serum transferrin was investigated in a pH range from 3.5 to 11.0 using time-resolved laser fluorescence spectroscopy (TRLFS). At pH [greater-than-or-equal] 7.4 Cm(iii) is incorporated at the Fe(iii) binding site of transferrin whereas at lower pH a partially bound Cm(iii) transferrin species is formed. At physiological temperature (310 K) at pH 7.4{,} about 70% of the partially bound and 30% of the incorporated Cm(iii) transferrin species are present in solution. The Cm(iii) results obtained by TRLFS are in very good agreement with Am(iii) EXAFS results{,} confirming the incorporation of Am(iii) at the Fe(iii) binding site at pH 8.5 ...
Nucleotide sequence of streptococcal pyrogenic exotoxin type C.: The nucleotide sequence of the gene speC, encoding streptococcal pyrogenic exotoxin type C (SPE
When it comes to milk, preventing bacterial contamination on dairy equipment is key. Researchers in Israel developed a biological coating to prevent biofilm formation and keep their moo-juice fresh and clean.. ...
Autovaletdirect specialises in the removal of particulate contamination on single or groups of vehicles from industrial emission or construction site overspray.
Learn all about what the P stands for in FODMAP! Polyols, also known as sugar alcohols can cause bloating and gas in people who have IBS. Learn more here.
... is a protein-separation technique that allows resolution of single proteins and other ampholytes from a complex mixture ...
... ampholyte mixtures MeSH D27.720.470.305 --- culture media MeSH D27.720.470.305.250 --- culture media, conditioned MeSH D27.720. ...
Immediate precipitation may occur giving a cloudy mixture. The solubility measured for such a mixture is known as "kinetic ... Solubility values of organic acids, bases, and ampholytes of pharmaceutical interest may be obtained by a process called " ... In static methods a mixture is brought to equilibrium and the concentration of a species in the solution phase is determined by ...
When a sample (a mixture of peptides or proteins) is injected in the capillary, the presence of the electrical field and the pH ... Molecules to be focused are distributed over a medium that has a pH gradient (usually created by aliphatic ampholytes). An ... IEF involves adding an ampholyte solution into immobilized pH gradient (IPG) gels. IPGs are the acrylamide gel matrix co- ... The multi-junction IEF system has been used to separate tryptic peptide mixtures for two-dimensional proteomics and blood ...
This method only gives a rough measure of the amounts of different proteins in the mixture, and it is not able to distinguish ... By making use of a pH-gradient, that can for example be induced by ampholytes, this technique allows to separate protein ... Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, ... When a vessel (typically a tube or bottle) containing a mixture of proteins or other particulate matter, such as bacterial ...
ISBN 978-1-4200-7099-6. Pagliara A, Carrupt PA, Caron G, Gaillard P, Testa, B. (1997). "Lipophilicity Profiles of Ampholytes ... is the ratio of concentrations of a compound in a mixture of two immiscible phases at equilibrium. This ratio is therefore a ...
Ampholyte Mixtures. Such mixtures of amphoteric Electrolytes or Buffers that provide a continuous range of pH in an electric ...
This study screened binary mixtures of pesticides for potential synergistic interaction effects on growth of the marine ... Ampholyte Mixtures. Such mixtures of amphoteric electrolytes or buffers that provide a continuous range of pH in an electric ... Complex Mixtures. Mixtures of many components in inexact proportions, usually natural, such as PLANT EXTRACTS; VENOMS; and ... The mixture of isoproturon and metazachlor tested on S. marinoi caused a 28-34% decrease in ϕ that was significantly higher ...
C11D1/88-Ampholytes; Electroneutral compounds * C11D1/94-Mixtures with anionic, cationic, or non-ionic compounds ... 375 g of water and 103.5 g of sodium monochloroacetate are added to mixture. The mixture is heated to 80° C. and a further 72.7 ... Surfactant mixtures having improved surface-active properties US4166048A (en) 1979-08-28. High foaming detergent composition ... A process for the production of detergent mixtures DE4340423C1 (en) 1993-11-27. 1995-07-13. Henkel Kgaa. Low viscosity aqueous ...
C11D1/88-Ampholytes; Electroneutral compounds * C11D1/94-Mixtures with anionic, cationic, or non-ionic compounds ... and mixtures thereof, and said surfactant selected from mild soaps and mild synthetic surfactants and mixtures thereof; and ... mixtures of ethoxylated alkyl sulfates and alkyl amine oxides, betaines, sultaines, and mixtures thereof. Included in the ... A preferred surfactant is selected from mild soaps and mild synthetic surfactants and mixtures thereof. Examples of these are ...
Here, poly(ampholytes), which contain a mixture of cationic and anionic side chains are quantitatively evaluated for their IRI ... ampholytes). The charge balance of the side chains is shown to be crucial, with only 50 : 50 mixtures having strong IRI ... Quantitative study on the antifreeze protein mimetic ice growth inhibition properties of poly(ampholytes) derived from vinyl- ... Quantitative study on the antifreeze protein mimetic ice growth inhibition properties of poly(ampholytes) derived from vinyl- ...
The ampholyte mixture pH 4-7 was purchased from AppliChem (Darmstadt, Germany) and the activated labelling dye Atto 425-NHS ... The online labelling reaction was performed as follows: biomolecules were dissolved in ampholyte solution (0.1% ampholyte pH 4- ... 3c). It should also be mentioned that an ampholyte with a pH of 4 to 7 was used, which is not well-suited for neurotensin with ... We used a 60 μL mixture of 85.0% (w/w) acryloylmorpholine, 14.8% (w/w) oligoethylene glycol (OEG-DA700), 0.02% (w/w) PBI as a ...
c)-d) application of free-flow isoelectric focusing: c) a wide sample mixture and ampholytes are sandwiched between two thin ... The sample mixture is injected into the carrier electrolyte flow and with increasing residence time the differently charged ... d) In the presence of an electrical field the ampholytes build up and buffer a linear pH gradient. Components move towards and ... The sample mixture is hydrodynamically focused by two sheath flow streams containing buffer only and no voltage is applied. b) ...
Lysates were mixed with ampholyte premix (pH 5-8) and fluorescent pI standards (pI Standard Ladder 3) before being loaded into ... Isoelectric focusing was performed in capillaries filled with a mixture of cell lysate (0.05-0.1 mg/ml protein), fluorescently ... labeled pI standards, and ampholytes. The separated proteins were cross-linked to the capillary wall using UV light followed by ...
... is a protein-separation technique that allows resolution of single proteins and other ampholytes from a complex mixture ...
However, in the case of the 35S labelled protein adducts only a small amount of the 35S incorporated into the gel mixture prior ... This was carried out as previously described [5] in 5% polyacrylamide gels, 2% ampholine carrier ampholytes pH 3.5 - 10 (LKB ... This was then evaporated to dryness in a rotary evaporator and the solid obtained dissolved in a 1:1 mixture of methanol and 10 ... the sample being incorporated directly into the gel mixture prior to polymerization. 10 mM H3PO4 was used for the anode ...
The mixtures containing 150 μg of protein were diluted 1:1 with rehydration buffer (7 m urea, 2 m thiourea, 4% CHAPS, 4% ... ampholytes (pH 3-11), and 200 mm DTT). The IPG strips (24 cm, pH 3-11 non-linear) were rehydrated overnight with 450 μl of a ... The seeded cells were incubated at 37 °C in a humidified gas mixture containing 5% CO2 balanced with air. The chondrocytes were ... Quantitative analysis of complex protein mixtures using isotope-coded affinity tags. Nat. Biotechnol. 17, 994- 999. ...
... protease inhibitor mixture, 1% broad range carrier ampholytes (pH 3-10), and 0.2% each of five narrow range ampholytes (pH 2.5- ... This mixture was then centrifuged at 12,000 × g for 15 min at 4 °C. The aqueous phase was transferred to a new tube, and the ... Uniformly, 100 μg of protein in solubilization buffer was combined 1:1 with an ampholyte buffer containing 8 m urea, 2 m ... To this mixture, 2.5 ml of isopropanol was added, the sample was mixed and incubated for 10 min, and the precipitated protein ...
... ampholyte mixtures MeSH D27.720.470.305 --- culture media MeSH D27.720.470.305.250 --- culture media, conditioned MeSH D27.720. ...
1 mixture of ampholytes with pH ranges of 2.5 to 5 and 4 to 6.5 (Pharmacia) at a final ampholyte concentration of 3%. The gel ...
ZM0018; Life Technologies] with a linear pH gradient 3-10 were rehydrated in 155 µL of resulting mixture overnight at room ... ampholyte, and trace amount of Bromophenol Blue]. Fixed immobilized precast IPG (immobilized pH gradient) strips [catalog (cat ...
A second washing with the mixture (100 uL) acetonitrile/TFA/water (01/10/1989) allowed extraction of the remaining peptides in ... ampholytes [pH 3 - 10] and DTT (65 mM) for 5 min at room temperature. The extract was centrifuged at 13,000 rpm for 30 minutes ... and the mixture was centrifuged at 4000 rpm for 1 hour at −20˚C. Then, the pellet was rinsed twice at −20˚C for 1 hour in 2- ... ampholytes and 20 mM DTT) for 13 h at 20˚C. The strips were protected from dehydration and oxidation by coating with mineral ...
Biolyte 3-10 ampholyte (BioRad) and 10%(v/v) glycerol. This mixture was focused with a constant power of 12 W for 5 hours at 4. ... For example, it iscommon to use a mixture of oligonucleotides as a probe for any particular sequence of amino acid, with each ... Such a library can be screened for useful clones by nucleic acid hybridization using theoligonucleotide mixtures described ... mixture in a 15 ml glass Corex test tube. The organiclayer was removed and dried under a stream of nitrogen gas. Residual ...
... carrier ampholyte mixture (IPG buffer; Pharmacia). Immobiline DryStrips (Pharmacia) were rehydrated overnight in rehydration ... was transformed with the ligation mixture and spread onto LB plates containing 50 μg of ampicillin per ml, 100 μM isopropyl β-D ...
... carrier ampholyte mixture (IPG Buffer; Pharmacia). Immobiline DryStrips (Pharmacia) were rehydrated overnight in rehydration ...
The sample mixture is injected to fill the entire capillary column. Voltage is then applied to the anolyte and catholyte tanks ... Protein samples are first premixed with carrier ampholytes, additives and pI markers. Samples are separated in a capillary ...
ABSTRACT Isoelectric focusing (IEF) polyacrylamide gel containing an 80% pH 4-6.5 and 20% pH 3-10 ampholyte mixture greatly ... pH 3-10 ampholyte mixture greatly improved protein banding pattern for species identification of water extracts of raw pink, ... Identification of a specific species in mixture samples was achieved by the detection of water‐extractable shrimp specific ... Identification of a specific species in mixture samples was achieved by the detection of water‐extractable shrimp specific ...
It can sort biomolecules at a relatively high flow rate of up to 10 microL/min and does not require carrier ampholytes, which ... 2016 , Pubmed ID: 27026280 Isolation of low abundance proteins or rare cells from complex mixtures, such as blood, is required ... we are evaluating the strategy of sorting peptides/proteins based on the charge to mass without resorting to ampholytes and/or ... we coupled two sorting steps via off-chip titration and isolated peptides within specific pI ranges from sample mixtures, where ...
Ampholyte Mixtures 両性電解質 Fatty Acids 脂肪酸 ...
Humans , In Vitro Techniques , Isoelectric Point , Ampholyte Mixtures/chemical synthesis , Proteins/blood , ... Síntesis y caracterización de anfolitos TEPA / Synthesis and characterization of carrier ampholytes TEPA ... Synthesis of carrier ampholytes and their applications to fractionate proteins of hemolysed erythrocytes ...
... of complex peptide mixtures in the absence of carrier ampholytes... Carrier ampholyte‐free , Mass spectrometry , Protein ... to prefractionate protein mixtures by carrier ampholyte‐free solution IEF... Ampholyte‐free solution isoelectric focusing , ... ampholytes are synthetic mixtures of low molecular mass amphoteric components that possess high buffering capacity, solubility ... Through the analysis of different test sample mixtures, it has been shown that the carrier ampholyte-based background ...
  • For example, in the manufacture of synthetic drugs, mixtures containing variable proportions of several compounds usually arise. (enacademic.com)
  • Method involves carrying out the following stages: a) preparing a solution or homogenous dispersion liquid and compound taken among the group including one or some pharmaceutically active compounds, one or some pharmaceutically acceptable additives and their mixtures, and the following stage b) foaming solution or homogenous dispersion at reducing pressure 30-150 torrs without boiling. (russianpatents.com)
  • This method involves the following stages (a) preparation of a solution or a homogeneous dispersion of liquids and compounds, selected from the group comprising one or more pharmaceutically active compounds, one or more pharmaceutically acceptable additives and their mixtures, and the subsequent b) foaming solution or homogeneous dispersion without boiling. (russianpatents.com)
  • A promising method in this respect is micro free-flow electrophoresis (μFFE) 2-4 because of its ability to continuously and preparatively separate and isolate biomolecules from mixtures on a small scale. (rsc.org)
  • Illustration of the µ-FFE device and applied separation methods: a), b) application of free-flow zone electrophoresis: a) The sample mixture is hydrodynamically focused by two sheath flow streams containing buffer only and no voltage is applied. (utwente.nl)
  • Professipnal titrate in the plane can be problematic and it is responsible to Online Perfopil Tablets Atlanta the ordinate, if relevant, in deionised hereditable or 2 per Purchase Viagra Professional 100mg Portland ampholytes, theorizing slippage or gel electrophoresis if continuous. (obyxova.ru)
  • Further, the aqueous gel medium comprises at least one ingredient selected from the group consisting of: carbomers, polymeric emulsifying agents, polysaccharidic biopolymers, gums, alginates, modified celluloses, starch derived products, polysorbate 80 (and) isohexadecane (and) acrylamide/sodium acryloyldimethyltaurate, and mixtures thereof. (cosmeticsandtoiletries.com)
  • 5 times of this solution, add the activity of ampholytes specified in the vacuum and Purchsae to 10 years with reference R. Mix officially and transfer the disease. (obyxova.ru)
  • Significant cases of antagonism (for both species) and synergism (for S. marinoi) were observed for the mixtures of isoproturon and spiroxamine, and isoproturon and metazachlor, respectively. (bioportfolio.com)
  • These two mixtures, together with that of isoproturon and diuron, for which additivity was observed, were further studied for their impacts on the physiology of each species. (bioportfolio.com)
  • Isoproturon and diuron singly and in mixtures induced 20-40% decreases in ϕ' which was in turn responsible for a significant decrease in relative lipid content for both species. (bioportfolio.com)
  • Proteins of the crushed seeds were precipitated for 2 hours 30 min at −20˚C in an acetone/TCA buffer containing 10% of TCA, 0.07% of 2-mercaptoethanol and acetone, and the mixture was centrifuged at 4000 rpm for 1 hour at −20˚C. Then, the pellet was rinsed twice at −20˚C for 1 hour in 2-mercaptoethanol and acetone buffer and centrifuged as previously done. (scirp.org)
  • The mixture of isoproturon and metazachlor tested on S. marinoi caused a 28-34% decrease in ϕ' that was significantly higher than levels induced by each of substances when tested alone. (bioportfolio.com)
  • This strong decrease in ϕ' could be due to a combined effect of these substances on the photosynthetic apparatus, which is likely the cause of the synergy found for this mixture. (bioportfolio.com)
  • The mixture of substances (proteins) to be fractionated is dissolved in a liquid or gaseous fluid called the mobile phase. (slideplayer.com)
  • First, the mixture may contain some substance that should be isolated from the rest of the mixture: this process of isolating and thus removing substances considered to be contaminants is called purification. (enacademic.com)
  • Convert units - Convert mol in quantity - Convert quantity in mol - Convert mass constituent in molarity - Convert molarity in mass constituent - Determine molarity - Determine molality - Solution of crystalline water substances - Mixture rules / cross - Solution of titrimetric standard substances 3. (lcs-chemie.de)
  • We tried the application of this method for the analysis of the ampholytes, which would be the most important question when the two technique is coupled sequentially. (mtak.hu)
  • By studying problems with the quantitative analysis of animal and vegetable oils and fats, the American Oil Chemists' Society has found certain mixtures to be useful as reference standards. (universalbiologicals.com)
  • This study screened binary mixtures of pesticides for potential synergistic interaction effects on growth of the marine microalgae Tisochrysis lutea and Skeletonema marinoi. (bioportfolio.com)
  • A poly(octadecyl acrylate-co-ethylene dimethacrylate) monolithic column served as the trap column to capture sample and to remove the ampholytes from CIEF. (dicp.ac.cn)
  • Calibrate LTQ Velos, LTQ Orbitrap and other mass spectrometers using a mixture of highly purified ionizable molecules designed for positive mode calibration. (fishersci.co.uk)
  • Exposures were thus made in culture flasks at three concentrations, or concentration combinations for mixtures, selected to cause 25%, 50% and 75% growth rate inhibition. (bioportfolio.com)
  • The removal of the desired drug from the rest of the mixture is important if the product is to have uniform potency and is to be free of other components that may be dangerous to the body. (enacademic.com)