Ampholyte Mixtures
Isoelectric Focusing
Dictionaries as Topic
Electrophoresis
Microfluidic Analytical Techniques
Cell Fractionation
Electrophoresis, Microchip
Microfluidics
Decapoda (Crustacea)
The largest order of CRUSTACEA, comprising over 10,000 species. They are characterized by three pairs of thoracic appendages modified as maxillipeds, and five pairs of thoracic legs. The order includes the familiar shrimps, crayfish (ASTACOIDEA), true crabs (BRACHYURA), and lobsters (NEPHROPIDAE and PALINURIDAE), among others.
Urea
Electrophoresis, Polyacrylamide Gel
Penaeidae
DNA Barcoding, Taxonomic
Adenosine-5'-O-phosphorylated and adenosine-5'-O-phosphorothioylated polyols as strong inhibitors of (symmetrical) and (asymmetrical) dinucleoside tetraphosphatases. (1/14)
Dinucleoside 5',5"'- P (1), P ( n )-polyphosphates, and particularly the diadenosine compounds, have been implicated in extracellular purinergic signalling and in various intracellular processes, including DNA metabolism, tumour suppression and stress responses. If permitted to accumulate, they may also be toxic. One approach to understanding their function is through the various specific degradative enzymes that regulate their levels. Eight adenosine-5'- O -phosphorylated polyols (derivatives of glycerol, erythritol and pentaerythritol) and 11 adenosine-5'- O -phosphorothioylated polyols (derivatives of glycerol, erythritol, pentaerythritol, butanediol and pentanediol) have been tested as inhibitors of specific diadenosine tetraphosphate (Ap(4)A) hydrolases. Of these two groups of novel nucleotides, the adenosine-5'- O -phosphorothioylated polyols were generally stronger inhibitors than their adenosine-5'- O -phosphorylated counterparts. 1,4-Di(adenosine-5'- O -phosphorothio) erythritol appeared to be the strongest inhibitor of ( asymmetrical ) Ap(4)A hydrolases (EC 3.6.1.17) from both lupin and human, with K (i) values of 0.15 microM and 1.5 microM respectively. Of eight adenosine-5'- O -phosphorylated polyols, 1,4-di(adenosine-5'- O -phospho) erythritol was the only compound that inhibited the lupin enzyme. Two derivatives of pentaerythritol, di(adenosine-5'- O -phosphorothio)-di(phosphorothio) pentaerythritol and tri(adenosine-5'- O -phosphorothio)-phosphorothio-pentaerythritol, proved to be the strongest inhibitors of the prokaryotic ( symmetrical ) Ap(4)A hydrolase (EC 3.6.1.41) so far reported. The estimated K (i) values were 0.04 microM and 0.08 microM respectively. All of these inhibitors were competitive with respect to Ap(4)A. These new selectively acting Ap(4)A analogues should prove to be valuable tools for further studies of Ap(4)A function and of the enzymes involved in its metabolism. (+info)Efficient solubilization buffers for two-dimensional gel electrophoresis of acidic and basic proteins extracted from wheat seeds. (2/14)
Plant tissues are made up of a broad range of proteins with a variety of properties. After extraction, solubilization of a diverse range of plant proteins for efficient proteomic analysis using two-dimensional electrophoresis is a challenging process. We tested the efficiency of 12 solubilization buffers in dissolving acidic and basic proteins extracted from mature seeds of wheat. The buffer containing two chaotropes (urea and thiourea), two detergents (3-[(3-cholamidopropyl) dimethyl-ammonio]-1-propane-sulfonate and N-decyl-N,N-dimethyl-3-ammonio-1-propane-sulfonate), two reducing agents (dithiothreitol and tris (2-carboxyethyl) phosphine hydrochloride) and two types of carrier ampholytes (BioLyte pH 4-6 and pH 3-10) solubilized the most acidic proteins in the pH range between 4 and 7. The buffer made up of urea, thiourea, 3-[(3-cholamidopropyl) dimethyl-ammonio]-1-propane-sulfonate, DeStreak reagent (Amersham Biosciences, Uppsala, Sweden) and immobilized pH gradient buffer, pH 6-11 (Amersham Biosciences) solubilized the most basic proteins in the pH range between 6 and 11. These two buffers produced two-dimensional gels with high resolution, superior quality and maximum number of detectable protein (1425 acidic protein and 897 basic protein) spots. (+info)Commercial ampholytes used for isoelectric focusing may interfere with bioactivity based purification of antimicrobial peptides. (3/14)
BioRad's Rotofor system has been frequently used for the purification of proteins and smaller peptides such as bacteriocins. In this study, we report that some commercially available ampholytes used with the Rotofor isoelectric focusing system possess antimicrobial activity, which may interfere with the purification of bacteriocins and bacteriocin-like substances. (+info)Infection with Panton-Valentine leukocidin-positive methicillin-resistant Staphylococcus aureus t034. (4/14)
(+info)Effect of synthetic carrier ampholytes on saturation of human serum transferrin. (5/14)
We have investigated the effect in solution of synthetic carrier ampholytes on the saturation of human serum transferrin. By spectrophotometric titrations of human serum transferrin with various Fe3+-carrier ampholyte solutions, we demonstrated that under these conditions carrier ampholytes behave as typical chelators, their binding curves being very similar to that obtained with disodium nitrilotriacetate. On performing titration experiments at three different pH values, carrier ampholytes act like nitrilotriacetate at pH 7.5, but the former are more effective iron donors at pH 8.4 and worse iron donors at pH 5.2. Spectrophotometric titrations of isolated C-terminal and N-terminal fragments obtained from human serum transferrin by thermolysin cleavage show no differences between them, and no differences with respect to the whole protein except that they contain half the number of binding sites. In order to determine a site-specificity of iron in the presence of ampholytes, the classical urea/polyacrylamide-gel-electrophoresis technique was adopted. Under saturating conditions carrier ampholyte solutions act mostly on the C-terminal site, whereas desaturating agents remove iron preferentially from the N-terminal site. Our findings support the hypothesis that Ampholine may chelate Fe3+ as well as many other compounds. (+info)Resolution of alkaline phosphatase isoenzymes in serum by isoelectric focusing in immobilized pH gradients. (6/14)
This new method for fractionation of serum alkaline phosphatase isoenzymes is based on isoelectric focusing on a mixed-type polyacrylamide support containing an immobilized pH gradient with a superimposed carrier-ampholyte gradient. All known forms of alkaline phosphatase are separated in an Immobiline pH 3.5-6.0 gradient, the sample being applied into pockets cast on a pH 8.0 plateau. Sharp zymogram bands are obtained by substituting alkaline-stable 5-bromo-4-chloro-3-indoxyl phosphate and tetrazolium salts for the standard 1- and 2-naphthyl phosphate-diazonium salt combinations. After hydrolysis of the phosphate group by the alkaline phosphatase the indoxyl moieties reduce tetrazolium salts to nearly insoluble and nondiffusible formazan precipitates. Normal sera show an array of about 10 isobands isoelectric between pH 3.9 and pH 4.79. In Paget's disease, two sharp isobands with pls of 4.97 and 5.09 are seen. Placental alkaline phosphatase overlaps with the higher pl bands of normal serum; however, upon heat destruction of the latter, it shows four sharp bands with the following pl's: 4.59, 4.62, 4.67 and 4.73. (+info)Charge microheterogeneity of the major capsid protein of polyoma virus. (7/14)
The behavior in isoelectric focusing of the major capsid polypeptide VPI of several strains of polyoma virus was studied. Two previously recognized phenomena were reexamined, namely, (i) the separation of the VP1 polypeptide into multiple subspecies differing only slightly from each other in apparent isoelectric point and (ii) strain differences in the overall apparent net charge of the family of VP1 subspecies. It was found that the pattern of subspecies was reproducible when focusing was initiated from either the basic or acidic region of the gel, keeping the ampholyte mixture constant. However, individual subspecies were unstable, and labeled polypeptide could be shifted dramatically by either refocusing of separated subspecies or by altering the concentration of ampholytes. These findings suggest that protein-protein and protein-ampholyte interactions play an important role in the generation of this charge heterogeneity. The basis for the overall charge difference between the VP1 of 3049 virus and several other strains (lpD, lpS, ts59, and A2) was studied, using recombinant viruses constructed of specific sequences derived from 3049 and lpD genomes. The portion of the VP1 polypeptide carrying the altered charge could be mapped to the body of the molecule 3' to the HindIII site at 45.0 map units (3,918 base pairs). This clearly segregates the VP1 charge phenotype from the cyc phenotype of 3049 in which capsid proteins are overproduced and accumulate in the cytoplasm of infected cells. (+info)pH-dependent aggregation and electrofocusing of poliovirus. (8/14)
Following isoelectric focusing, poliovirus can be detected at two pH values. The acidic form consists of poliovirus aggregates and the neutral form of single virions. (+info)
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Patent US4387113 - Method of producing electrical layer capacitors with glow-polymerizate ... - Google Patents
Isoelectric Focusing›Electrophoresis › SERVA Electrophoresis GmbH
Peptide Analysis and Characterization - Peptides | Sigma-Aldrich
adenosine-5-diphosphate-disodium-salt-adp-disodium-salt | VWR
adenosine-5-o-3-thiotriphosphate-tetralithium-salt | VWR
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ampholyte | Tabers Medical Dictionary
Ampholyte Mixtures - Medical Dictionary online-medical-dictionary.org
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Chromatofocusing
... is a protein-separation technique that allows resolution of single proteins and other ampholytes from a complex mixture ...
List of MeSH codes (D27)
... ampholyte mixtures MeSH D27.720.470.305 - culture media MeSH D27.720.470.305.250 - culture media, conditioned MeSH D27.720. ...
Solubility equilibrium
Immediate precipitation may occur giving a cloudy mixture. The solubility measured for such a mixture is known as "kinetic ... Solubility values of organic acids, bases, and ampholytes of pharmaceutical interest may be obtained by a process called " ... In static methods a mixture is brought to equilibrium and the concentration of a species in the solution phase is determined by ...
Immobilized pH gradient
... s (IPG) are made by mixing two kinds of acrylamide mixture, one with Immobiline having acidic buffering ... method eliminates the problems of gradient instability and poor sample loading capacity associated with carrier ampholyte pH ...
Isoelectric focusing
When a sample (a mixture of peptides or proteins) is injected in the capillary, the presence of the electrical field and the pH ... Molecules to be focused are distributed over a medium that has a pH gradient (usually created by aliphatic ampholytes). An ... IEF involves adding an ampholyte solution into immobilized pH gradient (IPG) gels. IPGs are the acrylamide gel matrix co- ... The multi-junction IEF system has been used to separate tryptic peptide mixtures for two-dimensional proteomics and blood ...
Protein purification
This method only gives a rough measure of the amounts of different proteins in the mixture, and it is not able to distinguish ... By making use of a pH-gradient, that can for example be induced by ampholytes, this technique allows to separate protein ... Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, ... When a vessel (typically a tube or bottle) containing a mixture of proteins or other particulate matter, such as bacterial ...
Partition coefficient
ISBN 978-1-4200-7099-6. Pagliara A, Carrupt PA, Caron G, Gaillard P, Testa B (1997). "Lipophilicity Profiles of Ampholytes". ... is the ratio of concentrations of a compound in a mixture of two immiscible solvents at equilibrium. This ratio is therefore a ...
Ampholyte Mixtures - Medical Dictionary online-medical-dictionary.org
Toxicity of binary mixtures of pesticides to the marine microalgae Tisochrysis lutea and Skeletonema marinoi: Substance...
This study screened binary mixtures of pesticides for potential synergistic interaction effects on growth of the marine ... Ampholyte Mixtures. Such mixtures of amphoteric electrolytes or buffers that provide a continuous range of pH in an electric ... Complex Mixtures. Mixtures of many components in inexact proportions, usually natural, such as PLANT EXTRACTS; VENOMS; and ... The mixture of isoproturon and metazachlor tested on S. marinoi caused a 28-34% decrease in ϕ that was significantly higher ...
US20030171229A1 - Concentrated aqueous betaine-type surfactant compositions and process for their preparation
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C11D1/88-Ampholytes; Electroneutral compounds * C11D1/94-Mixtures with anionic, cationic, or non-ionic compounds ... 375 g of water and 103.5 g of sodium monochloroacetate are added to mixture. The mixture is heated to 80° C. and a further 72.7 ... Surfactant mixtures having improved surface-active properties US4166048A (en) 1979-08-28. High foaming detergent composition ... A process for the production of detergent mixtures DE4340423C1 (en) 1993-11-27. 1995-07-13. Henkel Kgaa. Low viscosity aqueous ...
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C11D1/88-Ampholytes; Electroneutral compounds * C11D1/94-Mixtures with anionic, cationic, or non-ionic compounds ... and mixtures thereof, and said surfactant selected from mild soaps and mild synthetic surfactants and mixtures thereof; and ... mixtures of ethoxylated alkyl sulfates and alkyl amine oxides, betaines, sultaines, and mixtures thereof. Included in the ... A preferred surfactant is selected from mild soaps and mild synthetic surfactants and mixtures thereof. Examples of these are ...
Quantitative study on the antifreeze protein mimetic ice growth inhibition properties of poly(ampholytes) derived from vinyl...
Here, poly(ampholytes), which contain a mixture of cationic and anionic side chains are quantitatively evaluated for their IRI ... ampholytes). The charge balance of the side chains is shown to be crucial, with only 50 : 50 mixtures having strong IRI ... Quantitative study on the antifreeze protein mimetic ice growth inhibition properties of poly(ampholytes) derived from vinyl- ... Quantitative study on the antifreeze protein mimetic ice growth inhibition properties of poly(ampholytes) derived from vinyl- ...
Continuous on-chip fluorescence labelling, free-flow isoelectric focusing and marker-free isoelectric point determination of...
The ampholyte mixture pH 4-7 was purchased from AppliChem (Darmstadt, Germany) and the activated labelling dye Atto 425-NHS ... The online labelling reaction was performed as follows: biomolecules were dissolved in ampholyte solution (0.1% ampholyte pH 4- ... 3c). It should also be mentioned that an ampholyte with a pH of 4 to 7 was used, which is not well-suited for neurotensin with ... We used a 60 μL mixture of 85.0% (w/w) acryloylmorpholine, 14.8% (w/w) oligoethylene glycol (OEG-DA700), 0.02% (w/w) PBI as a ...
Microfluidic Free-Flow Electrophoresis for Proteomics on a chip
c)-d) application of free-flow isoelectric focusing: c) a wide sample mixture and ampholytes are sandwiched between two thin ... The sample mixture is injected into the carrier electrolyte flow and with increasing residence time the differently charged ... d) In the presence of an electrical field the ampholytes build up and buffer a linear pH gradient. Components move towards and ... The sample mixture is hydrodynamically focused by two sheath flow streams containing buffer only and no voltage is applied. b) ...
Chromatofocusing - Wikipedia
VEGFR2 induces c-Src signaling and vascular permeability in vivo via the adaptor protein TSAd | JEM
Lysates were mixed with ampholyte premix (pH 5-8) and fluorescent pI standards (pI Standard Ladder 3) before being loaded into ... Isoelectric focusing was performed in capillaries filled with a mixture of cell lysate (0.05-0.1 mg/ml protein), fluorescently ... labeled pI standards, and ampholytes. The separated proteins were cross-linked to the capillary wall using UV light followed by ...
List of MeSH codes (D27) - Wikipedia
Studies on the Thiol Components of Isolated Nuclei
However, in the case of the 35S labelled protein adducts only a small amount of the 35S incorporated into the gel mixture prior ... This was carried out as previously described [5] in 5% polyacrylamide gels, 2% ampholine carrier ampholytes pH 3.5 - 10 (LKB ... This was then evaporated to dryness in a rotary evaporator and the solid obtained dissolved in a 1:1 mixture of methanol and 10 ... the sample being incorporated directly into the gel mixture prior to polymerization. 10 mM H3PO4 was used for the anode ...
Plus it
The mixtures containing 150 μg of protein were diluted 1:1 with rehydration buffer (7 m urea, 2 m thiourea, 4% CHAPS, 4% ... ampholytes (pH 3-11), and 200 mm DTT). The IPG strips (24 cm, pH 3-11 non-linear) were rehydrated overnight with 450 μl of a ... The seeded cells were incubated at 37 °C in a humidified gas mixture containing 5% CO2 balanced with air. The chondrocytes were ... Quantitative analysis of complex protein mixtures using isotope-coded affinity tags. Nat. Biotechnol. 17, 994- 999. ...
Plus it
... protease inhibitor mixture, 1% broad range carrier ampholytes (pH 3-10), and 0.2% each of five narrow range ampholytes (pH 2.5- ... This mixture was then centrifuged at 12,000 × g for 15 min at 4 °C. The aqueous phase was transferred to a new tube, and the ... Uniformly, 100 μg of protein in solubilization buffer was combined 1:1 with an ampholyte buffer containing 8 m urea, 2 m ... To this mixture, 2.5 ml of isopropanol was added, the sample was mixed and incubated for 10 min, and the precipitated protein ...
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Loss of Ribosomal Protein L11 Blocks Stress Activation of the Bacillus subtilis Transcription Factor ςB | Journal of...
Neutrophil inhibitors - Patent # 5708141 - PatentGenius
Biolyte 3-10 ampholyte (BioRad) and 10%(v/v) glycerol. This mixture was focused with a constant power of 12 W for 5 hours at 4. ... For example, it iscommon to use a mixture of oligonucleotides as a probe for any particular sequence of amino acid, with each ... Such a library can be screened for useful clones by nucleic acid hybridization using theoligonucleotide mixtures described ... mixture in a 15 ml glass Corex test tube. The organiclayer was removed and dried under a stream of nitrogen gas. Residual ...
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iCE3 :: ProteinSimple
Extraction and Solubilization Methods Suitable for Proteomic Analysis of Common Bean Seed of Ivory Coast
A second washing with the mixture (100 uL) acetonitrile/TFA/water (01/10/1989) allowed extraction of the remaining peptides in ... ampholytes [pH 3 - 10] and DTT (65 mM) for 5 min at room temperature. The extract was centrifuged at 13,000 rpm for 30 minutes ... and the mixture was centrifuged at 4000 rpm for 1 hour at −20˚C. Then, the pellet was rinsed twice at −20˚C for 1 hour in 2- ... ampholytes and 20 mM DTT) for 13 h at 20˚C. The strips were protected from dehydration and oxidation by coating with mineral ...
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USE OF A MODIFIED UREA GEL ISOELECTRIC FOCUSING METHOD FOR SPECIES IDENTIFICATION OF RAW OR BOILED WHITE, PINK, AND ROCK SHRIMP...
ABSTRACT Isoelectric focusing (IEF) polyacrylamide gel containing an 80% pH 4-6.5 and 20% pH 3-10 ampholyte mixture greatly ... pH 3-10 ampholyte mixture greatly improved protein banding pattern for species identification of water extracts of raw pink, ... Identification of a specific species in mixture samples was achieved by the detection of water‐extractable shrimp specific ... Identification of a specific species in mixture samples was achieved by the detection of water‐extractable shrimp specific ...
Glycogen Synthase Kinase 3 Is an Insulin-Regulated C/EBPα Kinase | Molecular and Cellular Biology
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It can sort biomolecules at a relatively high flow rate of up to 10 microL/min and does not require carrier ampholytes, which ... 2016 , Pubmed ID: 27026280 Isolation of low abundance proteins or rare cells from complex mixtures, such as blood, is required ... we are evaluating the strategy of sorting peptides/proteins based on the charge to mass without resorting to ampholytes and/or ... we coupled two sorting steps via off-chip titration and isolated peptides within specific pI ranges from sample mixtures, where ...
Amphoteric behavior of complex systems. II. Titration of sulfanilic acid-glycine mixtures - CaltechAUTHORS
Protein Standards for IEF›Isoelectric Focusing›Electrophoresis › SERVA Electrophoresis GmbH
In routine analysis, precast gels with carrier ampholytes or with immobilized pH gradient are available to save time and effort ... Quite common is the pI determination via coelectrophoresis of known protein marker mixtures. By simply comparing the position ... IEF is preferably used for the characterization of complex protein mixtures, particularly, if their components are almost ...
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Humans , In Vitro Techniques , Isoelectric Point , Ampholyte Mixtures/chemical synthesis , Proteins/blood , ... Síntesis y caracterización de anfolitos TEPA / Synthesis and characterization of carrier ampholytes TEPA ... Synthesis of carrier ampholytes and their applications to fractionate proteins of hemolysed erythrocytes ...
Search Articles | University of Toronto Libraries
... of complex peptide mixtures in the absence of carrier ampholytes... Carrier ampholyte‐free , Mass spectrometry , Protein ... to prefractionate protein mixtures by carrier ampholyte‐free solution IEF... Ampholyte‐free solution isoelectric focusing , ... ampholytes are synthetic mixtures of low molecular mass amphoteric components that possess high buffering capacity, solubility ... Through the analysis of different test sample mixtures, it has been shown that the carrier ampholyte-based background ...
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12161701 - Ampholyte mixtures 12161702 - Bicarbonate buffers 12161703 - Other buffers 12161704 - Acid buffers 12161705 - Basic ... 12142106 - Inert gas mixtures 12150000 - Acids and their salts and esters 12151500 - Carboxylic acids and their salts and ... 12163000 - Mud removal mixtures 12163001 - Mud cleanout agents 12163100 - Anti sludgers 12163101 - Anti sludge additives ...
Protein mixturesPremixed with carrier ampholytesIsoelectricBuffersGradientElectrophoresisCompoundsPeptidesGlycineCationicPolymersBufferSubstancesSodiumComplexSeparationGradientsVacuumSamplesMarkerIdentificationCapillarySpeciesTemperatureSampleChemicalDetectionBehaviorAnalysisComponentsResolveStandardSolutionStudyActivityHighlyGrowthBandsColumnSize
Protein mixtures7
- Due to its high resolving power while retaining the native state, IEF is preferably used for the characterization of complex protein mixtures, particularly, if their components are almost indistinguishable, for example the isoforms of certain enzymes. (serva.de)
- Both, standard protein mixtures and also bacterial proteins were investigated. (mtak.hu)
- Martin RG, Ames BN (1961) A method for determining the sedimentation behavior of enzymes: application to protein mixtures. (springer.com)
- Whether used alone or as part of a broader separation or purification scheme, the MicroRotofor cell offers rapid, simple, and effective fractionation and enrichment of proteins from complex protein mixtures. (bio-rad.com)
- Two dimensional polyacrylamide gel electrophoresis (2-DE) is considered a powerful tool used for separation and fractionation of complex protein mixtures from tissues, cells, or other biological samples. (biomedcentral.com)
- 2-DE is a powerful and widely used method for analysis of complex protein mixtures with exceptional ability to separate thousands of proteins at once. (biomedcentral.com)
- This technique has become a routine method for DNA size fragmenting and separating protein mixtures in most laboratories around the world. (encyclopedia.pub)
Premixed with carrier ampholytes1
- Protein samples are first premixed with carrier ampholytes, additives and pI markers. (proteinsimple.com)
Isoelectric7
- Using this assembly, labelling of protein and peptide mixtures, their separation via free-flow isoelectric focusing and the determination of the isoelectric point (pI) of the separated products via the integrated sensor layer could be carried out within typically around 5 minutes. (rsc.org)
- c)-d) application of free-flow isoelectric focusing: c) a wide sample mixture and ampholytes are sandwiched between two thin sheath flow streams of high and low pH. (utwente.nl)
- Chromatofocusing is a protein-separation technique that allows resolution of single proteins and other ampholytes from a complex mixture according to differences in their isoelectric point. (wikipedia.org)
- MARSHALL, MAURICE R. 1990-04-01 00:00:00 ABSTRACT Isoelectric focusing (IEF) polyacrylamide gel containing an 80% pH 4-6.5 and 20% pH 3-10 ampholyte mixture greatly improved protein banding pattern for species identification of water extracts of raw pink, white and rock shrimp compared with the system using only the pH 3-10 range ampholyte. (deepdyve.com)
- This new method for fractionating alkaline phosphatase isoforms in hepatobiliary disorders is based on isoelectric focusing on a mixed-type polyacrylamide support containing an immobilized pH gradient with a superimposed carrier-ampholyte gradient. (elsevier.com)
- These isoforms focus in the pl 5-6 range (while alkaline phosphatases in normal serum focus in the pl 4-5 interval) in immobilized pH gradients, but behave as strongly acidic components by agarose isoelectric focusing in the presence of carrier ampholytes, suggesting that they are strongly complexed with the latter. (elsevier.com)
- however, some laboratories still use carrier ampholytes-based isoelectric focusing technique. (biomedcentral.com)
Buffers1
- The innovative format of this assay assures accurate quantitation from just 1 mL of sample in the presence of detergents, reducing agents and urea buffers containing up to 1% of carrier ampholytes. (thermofisher.com)
Gradient4
- d) In the presence of an electrical field the ampholytes build up and buffer a linear pH gradient. (utwente.nl)
- In routine analysis, precast gels with carrier ampholytes or with immobilized pH gradient are available to save time and effort and to provide reproducible, reliable results. (serva.de)
- Bio-Lyte ampholytes are required to form the internal pH gradient. (bio-rad.com)
- B)exhibits a stable pH gradient when ampholytes become distributed in an electric field. (quizplus.com)
Electrophoresis10
- A promising method in this respect is micro free-flow electrophoresis (μFFE) 2-4 because of its ability to continuously and preparatively separate and isolate biomolecules from mixtures on a small scale. (rsc.org)
- Illustration of the µ-FFE device and applied separation methods: a), b) application of free-flow zone electrophoresis: a) The sample mixture is hydrodynamically focused by two sheath flow streams containing buffer only and no voltage is applied. (utwente.nl)
- A mixture of 8 purified proteins and peptides (3.4 to 100kDa) for use as size standards in peptide and small protein gel electrophoresis. (fishersci.fi)
- A mixture of 11 proteins (5 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting. (fishersci.fi)
- A mixture of seven native proteins (14.4kDa to 116kDa) for use as size standards in protein electrophoresis (SDS-PAGE). (fishersci.fi)
- Mixture of 9 blue-, orange- and green-stained proteins (10 to 250kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting. (fishersci.fi)
- Electrophoresis is an analytical technique that has been applied to resolve complex mixtures containing DNA, proteins, and other chemical or biological species. (encyclopedia.pub)
- Microchip electrophoresis provides separations within a minute or few seconds while capillary electrophoresis takes from minutes to hours to fully resolve the components of the sample mixture. (encyclopedia.pub)
- Realities in resolution may be bad by perturbing immobilised pH centuries Sale filagra extra power 150 mg Anchorage the buffering species, which are involved to carrier ampholytes, Purchase Viagra Professional 100mg Portland copolymerised within the gel electrophoresis. (obyxova.ru)
- Professipnal titrate in the plane can be problematic and it is responsible to Online Perfopil Tablets Atlanta the ordinate, if relevant, in deionised hereditable or 2 per Purchase Viagra Professional 100mg Portland ampholytes, theorizing slippage or gel electrophoresis if continuous. (obyxova.ru)
Compounds1
- For example, in the manufacture of synthetic drugs, mixtures containing variable proportions of several compounds usually arise. (enacademic.com)
Peptides4
- The merits of hydrophobic interaction chromatography on octyl-Sepharose as a method for the separation of carrier ampholytes from peptides have been investigated. (uu.nl)
- A standard mixture of nine peptides containing approximately 550 pmol of total phosphopeptide was incubated with 400 mg of Captivate ferrofluid phosphopeptide-binding reagent. (thermofisher.com)
- Snake venom is a complex mixture of proteins and peptides, and a number of studies have described the biological properties of several venomous proteins. (biology-online.org)
- In contrast, Chp F formed short assemblies containing a mixture of random coil and β-sheet structure at a basic pH of 10.0, where only 40% of the peptides converted to fibrils. (mdpi.com)
Glycine2
- It is the purpose of this and the following paper to test portions of the theory in a more quantitative manner, and in this paper the mutual action of two ampholytes, glycine and sulfanilic acid, is studied by means of titration over a wide pH range. (caltech.edu)
- GE Healthcare Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals. (fishersci.co.uk)
Cationic1
- Here, poly(ampholytes), which contain a mixture of cationic and anionic side chains are quantitatively evaluated for their IRI activity. (rsc.org)
Polymers1
- According to the patent, the instant ampholyte polymers and polymer compositions condition and improve the skin and nails. (cosmeticsandtoiletries.com)
Buffer2
- After vacuum drying, Protein was extracted by vortexing 100 mg of seed powder with 1.5 ml of extraction buffer containing CHAPS (4%), urea (5 M), thiourea (2 M), (0.8%) ampholytes [pH 3 - 10] and DTT (65 mM) for 5 min at room temperature. (scirp.org)
- Proteins of the crushed seeds were precipitated for 2 hours 30 min at −20˚C in an acetone/TCA buffer containing 10% of TCA, 0.07% of 2-mercaptoethanol and acetone, and the mixture was centrifuged at 4000 rpm for 1 hour at −20˚C. Then, the pellet was rinsed twice at −20˚C for 1 hour in 2-mercaptoethanol and acetone buffer and centrifuged as previously done. (scirp.org)
Substances6
- The mixture of isoproturon and metazachlor tested on S. marinoi caused a 28-34% decrease in ϕ' that was significantly higher than levels induced by each of substances when tested alone. (bioportfolio.com)
- This strong decrease in ϕ' could be due to a combined effect of these substances on the photosynthetic apparatus, which is likely the cause of the synergy found for this mixture. (bioportfolio.com)
- The mixture of substances (proteins) to be fractionated is dissolved in a liquid or gaseous fluid called the mobile phase. (slideplayer.com)
- Separation methods, then, can be defined as processes that change the relative amounts of substances in a mixture. (enacademic.com)
- First, the mixture may contain some substance that should be isolated from the rest of the mixture: this process of isolating and thus removing substances considered to be contaminants is called purification. (enacademic.com)
- Convert units - Convert mol in quantity - Convert quantity in mol - Convert mass constituent in molarity - Convert molarity in mass constituent - Determine molarity - Determine molality - Solution of crystalline water substances - Mixture rules / cross - Solution of titrimetric standard substances 3. (lcs-chemie.de)
Sodium1
- Further, the aqueous gel medium comprises at least one ingredient selected from the group consisting of: carbomers, polymeric emulsifying agents, polysaccharidic biopolymers, gums, alginates, modified celluloses, starch derived products, polysorbate 80 (and) isohexadecane (and) acrylamide/sodium acryloyldimethyltaurate, and mixtures thereof. (cosmeticsandtoiletries.com)
Complex1
- Proteomics now makes it possible to globally identify proteins from a complex mixture. (biology-online.org)
Separation1
- A separation of a somatomedin-containing peptide mixture into seven fractions by hydrophobic interaction chromatography on octyl-Sepharose is shown. (uu.nl)
Gradients1
- A mixture of human serum albumin (pI 4.6) and beta-lactoglobulin (pI 5.22) was separated in pH gradients, generated with carrier ampholytes of different origin and covering the pH ranges 4-6.5, 3.5-5, 4-5.5 and 4.5-5.0. (courtfield.ml)
Vacuum1
- 5 times of this solution, add the activity of ampholytes specified in the vacuum and Purchsae to 10 years with reference R. Mix officially and transfer the disease. (obyxova.ru)
Samples1
- Identification of a specific species in mixture samples was achieved by the detection of water‐extractable shrimp specific protein bands present in the gel. (deepdyve.com)
Marker1
- Quite common is the pI determination via coelectrophoresis of known protein marker mixtures. (serva.de)
Identification1
- This fatty acid reference mixture contains saturated and mono and polyunsaturated fatty acid methyl esters for the qualitative identification and quantitation of unknowns. (universalbiologicals.com)
Capillary1
- The sample mixture is injected to fill the entire capillary column. (proteinsimple.com)
Species3
- Significant cases of antagonism (for both species) and synergism (for S. marinoi) were observed for the mixtures of isoproturon and spiroxamine, and isoproturon and metazachlor, respectively. (bioportfolio.com)
- These two mixtures, together with that of isoproturon and diuron, for which additivity was observed, were further studied for their impacts on the physiology of each species. (bioportfolio.com)
- Isoproturon and diuron singly and in mixtures induced 20-40% decreases in ϕ' which was in turn responsible for a significant decrease in relative lipid content for both species. (bioportfolio.com)
Temperature1
- Daily temperature variation magnifies the toxicity of a mixture consisting of a chemical pesticide and a biopesticide in a vector mosquito. (bioportfolio.com)
Sample2
- The sample mixture is injected into the carrier electrolyte flow and with increasing residence time the differently charged components split up into diverging lanes which can be collected at various device outlets, as shown in Fig. 1 . (utwente.nl)
- A poly(octadecyl acrylate-co-ethylene dimethacrylate) monolithic column served as the trap column to capture sample and to remove the ampholytes from CIEF. (dicp.ac.cn)
Chemical1
- The contaminants, instead of single contaminants, are usually found as chemical mixtures in terrestrial environment. (bioportfolio.com)
Detection1
- The proteins were then measured with the Maurice imaged CIEF system (native fluorescence detection), testing various measurement conditions, such as different ampholytes or ampholyte mixtures. (bvsalud.org)
Behavior1
- The University of Michigan Medical School, Ann Arbor), and Jane E. Experimental behavior is reproduced by computer simulation of a model mixture of 15 hypothetical carrier ampholytes whose pIs span the pH range 3-10. (wearburn.info)
Analysis2
- We tried the application of this method for the analysis of the ampholytes, which would be the most important question when the two technique is coupled sequentially. (mtak.hu)
- By studying problems with the quantitative analysis of animal and vegetable oils and fats, the American Oil Chemists' Society has found certain mixtures to be useful as reference standards. (universalbiologicals.com)
Components1
- The removal of the desired drug from the rest of the mixture is important if the product is to have uniform potency and is to be free of other components that may be dangerous to the body. (enacademic.com)
Resolve1
- 02 pH tings may be very riding a Profesaional prepared with carrier ampholytes while immobilised pH widths can resolve proteins differing by approximately 0. (obyxova.ru)
Standard1
- This AOCS RM mixture is especially ideal as a standard for linseed, perilla, hempseed, and rubberseed oils. (universalbiologicals.com)
Solution1
- 40. 5 volumes of this solution, add the mixture Hindgra sildenafil citrate ampholytes specified Hindgra sildenafil citrate the monograph and dilute to 10 volumes with water R. (shaymuratovo.ru)
Study1
- This study screened binary mixtures of pesticides for potential synergistic interaction effects on growth of the marine microalgae Tisochrysis lutea and Skeletonema marinoi. (bioportfolio.com)
Activity1
- The charge balance of the side chains is shown to be crucial, with only 50 : 50 mixtures having strong IRI activity, which also scales with molecular weight. (rsc.org)
Highly1
- Calibrate LTQ Velos, LTQ Orbitrap and other mass spectrometers using a mixture of highly purified ionizable molecules designed for positive mode calibration. (fishersci.co.uk)
Growth1
- Exposures were thus made in culture flasks at three concentrations, or concentration combinations for mixtures, selected to cause 25%, 50% and 75% growth rate inhibition. (bioportfolio.com)
Bands1
- Interferometer between water bands on an IEF gel prepared with Professilnal ampholytes can be efficiently good. (obyxova.ru)
Column1
- The protein mixture is bound to the topmost portionof the ion exchanger in the chromatography column. (slideplayer.com)
Size1
- Working with proteins In a mixture of the five proteins listed below,which should elute second in size-exclusion (gel- filtration)chromatography? (quizplus.com)