Biocompatible Materials: Synthetic or natural materials, other than DRUGS, that are used to replace or repair any body TISSUES or bodily function.Vagina: The genital canal in the female, extending from the UTERUS to the VULVA. (Stedman, 25th ed)Electric Power Supplies: Devices that control the supply of electric current for running electrical equipment.Electronic Mail: Messages between computer users via COMPUTER COMMUNICATION NETWORKS. This feature duplicates most of the features of paper mail, such as forwarding, multiple copies, and attachments of images and other file types, but with a speed advantage. The term also refers to an individual message sent in this way.Patents as Topic: Exclusive legal rights or privileges applied to inventions, plants, etc.Housing: Living facilities for humans.Bioelectric Energy Sources: Electric power supply devices which convert biological energy, such as chemical energy of metabolism or mechanical energy of periodic movements, into electrical energy.NewsNewspapers: Publications printed and distributed daily, weekly, or at some other regular and usually short interval, containing news, articles of opinion (as editorials and letters), features, advertising, and announcements of current interest. (Webster's 3d ed)Mass Media: Instruments or technological means of communication that reach large numbers of people with a common message: press, radio, television, etc.Truth Disclosure: Truthful revelation of information, specifically when the information disclosed is likely to be psychologically painful ("bad news") to the recipient (e.g., revelation to a patient or a patient's family of the patient's DIAGNOSIS or PROGNOSIS) or embarrassing to the teller (e.g., revelation of medical errors).Journalism, Medical: The collection, writing, and editing of current interest material on topics related to biomedicine for presentation through the mass media, including newspapers, magazines, radio, or television, usually for a public audience such as health care consumers.Television: The transmission and reproduction of transient images of fixed or moving objects. An electronic system of transmitting such images together with sound over a wire or through space by apparatus that converts light and sound into electrical waves and reconverts them into visible light rays and audible sound. (From Webster, 3rd ed)Journalism: The collection, preparation, and distribution of news and related commentary and feature materials through such media as pamphlets, newsletters, newspapers, magazines, radio, motion pictures, television, and books. While originally applied to the reportage of current events in printed form, specifically newspapers, with the advent of radio and television the use of the term has broadened to include all printed and electronic communication dealing with current affairs.Embolism, Amniotic Fluid: Blocking of maternal circulation by AMNIOTIC FLUID that is forced into uterine VEINS by strong UTERINE CONTRACTION near the end of pregnancy. It is characterized by the sudden onset of severe respiratory distress and HYPOTENSION that can lead to maternal DEATH.Disseminated Intravascular Coagulation: A disorder characterized by procoagulant substances entering the general circulation causing a systemic thrombotic process. The activation of the clotting mechanism may arise from any of a number of disorders. A majority of the patients manifest skin lesions, sometimes leading to PURPURA FULMINANS.Cesarean Section: Extraction of the FETUS by means of abdominal HYSTEROTOMY.Pregnancy: The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.Pubic Symphysis: A slightly movable cartilaginous joint which occurs between the pubic bones.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Oligohydramnios: A condition of abnormally low AMNIOTIC FLUID volume. Principal causes include malformations of fetal URINARY TRACT; FETAL GROWTH RETARDATION; GESTATIONAL HYPERTENSION; nicotine poisoning; and PROLONGED PREGNANCY.Thoracic Cavity: The region of the thorax that includes the PLEURAL CAVITY and MEDIASTINUM.Nevi and Melanomas: A collective term for the various types of nevi and melanomas.Phenyl Ethers: Ethers that are linked to a benzene ring structure.Hernia, Diaphragmatic: Protrusion of abdominal structures into the THORAX as a result of congenital or traumatic defects in the respiratory DIAPHRAGM.Emergencies: Situations or conditions requiring immediate intervention to avoid serious adverse results.Emergency Treatment: First aid or other immediate intervention for accidents or medical conditions requiring immediate care and treatment before definitive medical and surgical management can be procured.Emergency Medicine: The branch of medicine concerned with the evaluation and initial treatment of urgent and emergent medical problems, such as those caused by accidents, trauma, sudden illness, poisoning, or disasters. Emergency medical care can be provided at the hospital or at sites outside the medical facility.Emergency Service, Hospital: Hospital department responsible for the administration and provision of immediate medical or surgical care to the emergency patient.Emergency Medical Services: Services specifically designed, staffed, and equipped for the emergency care of patients.Hospital Rapid Response Team: Multidisciplinary team most frequently consisting of INTENSIVE CARE UNIT trained personnel who are available 24 hours per day, 7 days per week for evaluation of patients who develop signs or symptoms of severe clinical deterioration.Emergency Medical Service Communication Systems: The use of communication systems, such as telecommunication, to transmit emergency information to appropriate providers of health services.Victoria: A state in southeastern Australia, the southernmost state. Its capital is Melbourne. It was discovered in 1770 by Captain Cook and first settled by immigrants from Tasmania. In 1851 it was separated from New South Wales as a separate colony. Self-government was introduced in 1851; it became a state in 1901. It was named for Queen Victoria in 1851. (From Webster's New Geographical Dictionary, 1988, p1295 & Room, Brewer's Dictionary of Names, p574)Body Image: Individuals' concept of their own bodies.GlobulinsSpecialization: An occupation limited in scope to a subsection of a broader field.Medicine: The art and science of studying, performing research on, preventing, diagnosing, and treating disease, as well as the maintenance of health.Serum Globulins: All blood proteins except albumin ( = SERUM ALBUMIN, which is not a globulin) and FIBRINOGEN (which is not in the serum). The serum globulins are subdivided into ALPHA-GLOBULINS; BETA-GLOBULINS; and GAMMA-GLOBULINS on the basis of their electrophoretic mobilities. (From Dorland, 28th ed)Sexuality: The sexual functions, activities, attitudes, and orientations of an individual. Sexuality, male or female, becomes evident at PUBERTY under the influence of gonadal steroids (TESTOSTERONE or ESTRADIOL), and social effects.Amniotic Fluid: A clear, yellowish liquid that envelopes the FETUS inside the sac of AMNION. In the first trimester, it is likely a transudate of maternal or fetal plasma. In the second trimester, amniotic fluid derives primarily from fetal lung and kidney. Cells or substances in this fluid can be removed for prenatal diagnostic tests (AMNIOCENTESIS).Schizophrenia, Paranoid: A chronic form of schizophrenia characterized primarily by the presence of persecutory or grandiose delusions, often associated with hallucination.Appointments and Schedules: The different methods of scheduling patient visits, appointment systems, individual or group appointments, waiting times, waiting lists for hospitals, walk-in clinics, etc.Paranoid Disorders: Chronic mental disorders in which there has been an insidious development of a permanent and unshakeable delusional system (persecutory delusions or delusions of jealousy), accompanied by preservation of clear and orderly thinking. Emotional responses and behavior are consistent with the delusional state.Body Fluids: Liquid components of living organisms.Follicular Fluid: The fluid surrounding the OVUM and GRANULOSA CELLS in the Graafian follicle (OVARIAN FOLLICLE). The follicular fluid contains sex steroids, glycoprotein hormones, plasma proteins, mucopolysaccharides, and enzymes.Stem Cells: Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.Stem Cell Transplantation: The transfer of STEM CELLS from one individual to another within the same species (TRANSPLANTATION, HOMOLOGOUS) or between species (XENOTRANSPLANTATION), or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS). The source and location of the stem cells determines their potency or pluripotency to differentiate into various cell types.AustriaEmbryonic Stem Cells: Cells derived from the BLASTOCYST INNER CELL MASS which forms before implantation in the uterine wall. They retain the ability to divide, proliferate and provide progenitor cells that can differentiate into specialized cells.Amnion: The innermost membranous sac that surrounds and protects the developing embryo which is bathed in the AMNIOTIC FLUID. Amnion cells are secretory EPITHELIAL CELLS and contribute to the amniotic fluid.MARVEL Domain Containing 2 Protein: A tight junction-associated MARVEL protein that may play a role in separating the endolymphatic and perilymphatic spaces of the ORGAN OF CORTI. Defects in the gene that codes for MARVELD2 protein are a cause of deafness autosomal recessive type 49.

Prenatal diagnosis of a lean umbilical cord: a simple marker for the fetus at risk of being small for gestational age at birth. (1/1267)

OBJECTIVE: The purpose of this study was to investigate whether the prenatal diagnosis of a 'lean' umbilical cord in otherwise normal fetuses identifies fetuses at risk of being small for gestational age (SGA) at birth and of having distress in labor. The umbilical cord was defined as lean when its cross-sectional area on ultrasound examination was below the 10th centile for gestational age. METHOD: Pregnant women undergoing routine sonographic examination were included in the study. Inclusion criteria were gestational age greater than 20 weeks, intact membranes, and singleton gestation. The sonographic cross-sectional area of the umbilical cord was measured in a plane adjacent to the insertion into the fetal abdomen. Umbilical artery Doppler waveforms were recorded during fetal apnea and fetal anthropometric parameters were measured. RESULTS: During the study period, 860 patients met the inclusion criteria, of whom 3.6% delivered a SGA infant. The proportion of SGA infants was higher among fetuses who had a lean umbilical cord on ultrasound examination than among those with a normal umbilical cord (11.5% vs. 2.6%, p < 0.05). Fetuses with a lean cord had a risk 4.4-fold higher of being SGA at birth than those with a normal umbilical cord. After 25 weeks of gestation, this risk was 12.4 times higher when the umbilical cord was lean than when it was of normal size. The proportion of fetuses with meconium-stained amniotic fluid at delivery was higher among fetuses with a lean cord than among those with a normal umbilical cord (14.6% vs. 3.1%, p < 0.001). The proportion of infants who had a 5-min Apgar score < 7 was higher among those who had a lean cord than among those with normal umbilical cord (5.2% vs. 1.3%, p < 0.05). Considering only patients admitted in labor with intact membranes and who delivered an appropriate-for-gestational-age infant, the proportion of fetuses who had oligohydramnios at the time of delivery was higher among those who had a lean cord than among those with a normal umbilical cord (17.6% versus 1.3%, p < 0.01). CONCLUSION: We conclude that fetuses with a lean umbilical cord have an increased risk of being small for gestational age at birth and of having signs of distress at the time of delivery.  (+info)

The 'Mickey Mouse' sign and the diagnosis of anencephaly in early pregnancy. (2/1267)

OBJECTIVES: To assess the sonographic screening for anencephaly in the first trimester in a low-risk obstetric population. METHODS: Since 1994, 5388 women attended our clinic for a first-trimester scan (11-14 weeks of gestation) and screening for structural and chromosomal abnormalities. The patients underwent transabdominal scanning, and transvaginal scanning if necessary. RESULTS: The ultrasonographic appearances of anencephaly in the first trimester are different from the familiar second-trimester signs. The cerebral hemispheres are present and exposed to the surrounding amniotic fluid. The ultrasound appearances in the coronal section of the head are best described as 'Mickey Mouse face'. There were six cases of anencephaly (incidence 1.1:1000). All cases were diagnosed in the first trimester and five demonstrated this sign. There were no false-positive diagnoses. The crown-rump length was significantly reduced in all affected fetuses. CONCLUSION: First-trimester ultrasonographic diagnosis of anencephaly is accurate, but sonographers should be familiar with the ultrasound appearances that are different from those in the second trimester.  (+info)

Adrenomedullin production is increased in normal human pregnancy. (3/1267)

OBJECTIVE: Adrenomedullin, a recently discovered vasoactive peptide originally identified in pheochromocytoma, has been found to be increased in the plasma of pregnant women at term. This study was designed to elucidate whether adrenomedullin secretion is dependent on gestational age and the possible source and function of this peptide in human pregnancy. STUDY DESIGN: Adrenomedullin concentrations were determined by RIA in amniotic fluid and maternal plasma obtained from 110 pregnant women between 8 and 40 weeks of gestation. Subjects were stratified into five groups according to gestational age. In term patients (n = 15), adrenomedullin was also measured in the umbilical artery and vein separately. RESULTS: High concentrations of adrenomedullin were present in plasma and amniotic fluid samples from patients in the first, second and third trimester. There was no significant difference in mean maternal plasma concentration of adrenomedullin between the five patient groupings. Amniotic fluid adrenomedullin concentrations decreased from 81.2 +/- 11.7 pg/ml at 8-12 weeks of gestation to 63.7 +/- 6.0 pg/ml at 13-20 weeks of gestation and then increased at 21-28 weeks of gestation to 99.1 +/- 10.4 pg/ml. A further increase was found in samples collected after 37 weeks of gestation (132.6 +/- 10.1 pg/ml). In the umbilical vein, adrenomedullin concentration was higher (P < 0.05) than in the artery (65.7 +/- 6.1 pg/ml and 48.5 +/- 5.2 pg/ml respectively), suggesting that adrenomedullin in the fetal circulation derives from the placenta. CONCLUSIONS: Our results demonstrate the presence of adrenomedullin in maternal plasma and amniotic fluid throughout gestation, and show that its production starts very early in gestation, suggesting that this hormone may have an important role in human reproduction, from implantation to delivery.  (+info)

Amniotic fluid concentrations of dimeric inhibins, activin A and follistatin in pregnancy. (4/1267)

OBJECTIVE: The feto-placental unit is the major source of circulating concentrations of inhibin A and activin A in human pregnancy. The aim of this study was to measure the amniotic fluid concentrations of inhibin A, inhibin B, activin A and follistatin in pregnancies bearing male and female fetuses. DESIGN AND METHOD: Amniotic fluid samples collected by amniocentesis were stored at -20 degrees C. Dimeric inhibins, 'total' activin A and 'total' follistatin were measured using specific two-site enzyme immunoassays. Samples were assayed blindly and the information on fetal sex was obtained from the cytogenetics laboratory. RESULTS: Data show that amniotic fluid concentrations of inhibin A, inhibin B and activin A gradually increase with gestation whilst concentrations of follistatin are similar between weeks 15 and 20 of pregnancy. Mean amniotic fluid levels of inhibin A and inhibin B at 16 and 17 weeks gestation and mean activin A levels at 15 and 16 weeks gestation are considerably lower in pregnancies with male (n=24) compared with female (n=28) fetuses. Levels of follistatin are not different in the male and female fetal pregnancies at any studied gestation. CONCLUSIONS: The results indicate that amniotic fluid contains high concentrations of inhibins (A and B), activin A and follistatin in early pregnancy suggesting that these hormones are produced by the fetal membranes and may be involved in the development of the fetus.  (+info)

SMN protein analysis in fibroblast, amniocyte and CVS cultures from spinal muscular atrophy patients and its relevance for diagnosis. (5/1267)

Spinal muscular atrophy (SMA) is an autosomal recessive neuromuscular disorder caused by the homozygous absence of the telomeric copy of the survival motor neuron (SMNt) gene, due to deletion, gene conversion or point mutation. SMNt and its homologous centromeric copy (SMNc) encode the SMN protein, which is diffusely present in the cytoplasm and in dot-like structures, called gems, in the nucleus. We have studied the SMN protein in different cell cultures, including fibroblasts, amniocytes and CVS cells from SMA individuals and controls. By immunofluorescence analysis we found a marked reduction in the number of gems in fibroblasts, amniocytes and chorionic villus cells of all SMA patients and foetuses, independent of the type of the genetic defect. We also show that immunolocalisation of the SMN protein may be a useful tool for the characterisation of particular patients of uncertain molecular diagnosis.  (+info)

Secretory leukocyte protease inhibitor concentration increases in amniotic fluid with the onset of labour in women: characterization of sites of release within the uterus. (6/1267)

Secretory leukocyte protease inhibitor is a potent inhibitor of neutrophil function, a mediator of mucosal immunity and an inhibitor of NF|gkB regulated inflammatory responses. However, its source, function and regulation within the uterus during pregnancy and at parturition are not well defined. In amniotic fluid, the concentration of secretory leukocyte protease inhibitor increased significantly from 2nd trimester (24+/-3 ng/ml; mean+/-s.e.m.; n=20) to term (751+/-53 ng/ml; P<0.05; n=15) with a further profound increase (P<0.005) with the onset of labour (3929+/-1076 ng/ml; n=15). To establish the intra-uterine sites of secretion, explants (n=6 different patients per tissue) were collected at term after elective caesarean section. High levels of secretory leukocyte protease inhibitor were released by decidua (135.2+/-12.4 pg/mg; mean+/-s.e.m.) and chorio-decidua (325.1+/-26.4 pg/mg) with less by amnion (55.6+/-6.0 pg/mg) and placenta (9.2+/-1.9 pg/mg). Intense immunoreactivity for secretory leukocyte protease inhibitor was detected predominantly in decidua parietalis cells adherent to the chorion laeve and myometrium, and also in decidua basalis. We propose that, within the pregnant uterus, secretory leukocyte protease inhibitor is released by decidua, fetal membranes and potentially the fetal lung. The increase in secretory leukocyte protease inhibitor may act to modulate pro-inflammatory paracrine interactions for the maintenance of pregnancy and limit those occurring at parturition within the uterus.  (+info)

Secretion of matrix metalloproteinase-2, matrix metalloproteinase-9 and tissue inhibitor of metalloproteinases into the intrauterine compartments during early pregnancy. (7/1267)

Matrix metalloproteinases (MMPs) are important enzymes in tissue remodelling, a key event for the development of the fetal membranes and placenta and establishing the feto-maternal interface during early pregnancy. This study has examined the secretion of the gelatinases, MMP-2 (72 kDa) and MMP-9 (92 kDa), and the endogenous tissue inhibitors of metalloproteinases (TIMPs) into extra-embryonic coelomic and amniotic fluids, the two principal intra-uterine compartments of the first trimester, and compared them to amniotic fluid collected later in gestation. In extra-embryonic coelomic fluid, gelatin zymography demonstrated that MMP-2 (72 kDa) was the predominant gelatinase, with some MMP-9 present. A broad range of TIMPs corresponding to TIMP-1 and TIMP-2, glycosylated and unglycosylated TIMP-3 and TIMP-4 was detected in this compartment by reverse zymography and immunoblot analyses. There was little gelatinase or TIMP activity in amniotic fluid in the first trimester. In amniotic fluid from the second trimester after fusion of the membranes obliterating the extra-embryonic coelom, and at term elective caesarean section, MMP-2 is the predominant gelatinase present, with a broad spectrum of TIMPs. These findings demonstrate that predominantly MMP-2 and also MMP-9, regulated by a range of TIMPs, are involved in intra-uterine tissue remodelling during the establishment of pregnancy.  (+info)

Vascular endothelial growth factor is bound in amniotic fluid and maternal serum. (8/1267)

To study vascular endothelial growth factor (VEGF) and placenta growth factor (PlGF) concentrations and their possible binders, serum from 22 non-pregnant and 55 pregnant women (15 at weeks 10-13; 40 at term), umbilical vein (n = 24) and artery (n = 13) and amniotic fluid (a pool of 50 at weeks 15-17; 11 at term) were assessed for VEGF and PlGF by an enzyme-linked immunosorbent assay. In amniotic fluid and maternal serum VEGF concentrations were <16 ng/ml and added VEGF was not recovered. VEGF was detected in serum from mothers post-partum (137 +/- 142 ng/l, mean +/- SD), umbilical artery (421 +/- 288 ng/l) and vein (502 +/- 339 ng/l) and non-pregnant controls (182 +/- 147 ng/l), and added VEGF was fully recovered. PlGF was detected in pregnancy serum (52 +/- 23 ng/l early pregnancy; 439 +/- 217 ng/l term pregnancy) and in amniotic fluid (early pregnancy 56 ng/l; term pregnancy 30 +/- 18 ng/l). PlGF was fully recovered in all samples. Gel filtration and isoelectric focusing revealed that in maternal serum and amniotic fluid [125I]VEGF was bound to a protein with an Mr of 400-700 kDa and an isoelectric point of approximately 8. This protein was not identical with alpha-2-macroglobulin (by an immunofluorometric assay), pregnancy zone protein or pregnancy associated plasma protein-A (by immunodiffusion). In conclusion, VEGF-binding activity is present in amniotic fluid and maternal blood. It disappears after delivery and is not detectable in fetal or non-pregnant serum.  (+info)

TY - JOUR. T1 - Human Amniotic Fluid Stem Cells. T2 - Therapeutic Potential for Perinatal Patients with Intractable Neurological Disease. AU - Ochiai, Daigo. AU - Masuda, Hirotaka. AU - Abe, Yushi. AU - Otani, Toshimitsu. AU - Fukutake, Marie. AU - Matsumoto, Tadashi. AU - Miyakoshi, Kei. AU - Tanaka, Mamoru. PY - 2018/12/26. Y1 - 2018/12/26. N2 - Mesenchymal stem cells (MSCs) have generated great interest in the fields of regenerative medicine and immunotherapy because of their unique biological properties. Among MSCs, amniotic fluid stem cells (AFS) have a number of characteristics that make them attractive candidates for tissue engineering and cell replacement strategies, particularly for perinatal medicine. If various neonatal conditions, including birth asphyxia, preterm birth, and congenital abnormalities, which result in long-lasting severe impairments, could be predicted during pregnancy, it would allow collection of small samples of amniotic fluid cells by amniocentesis. In vitro ...
Firstly, we analyzed hAFC samples for the expression of germ cell markers; in agreement to previous reports [11], we observed a consistently high expression of OCT4 and CD133. However, when we examined additional markers we did not observe expression of genes specific to germ cell differentiation. In this regard, it was reported that hAFCs had the ability to form embryoid bodies (EBs) when cultured under conditions without anti-differentiation factors and without contact to feeder cells. Moreover, the formation of such three-dimensional multicellular aggregates was accompanied by a decrease in stem cell marker expression and by the induction of differentiation into different cell lineages [23]. Hence herein hAFCs kept under a stem cell culture system yielded a high number of clones that when cultured in suspension without anti-differentiation factors, spontaneously formed multicellular EBs and harbored high differentiation potential. Moreover, when we cultured EBs in the presence of germ cell ...
TY - JOUR. T1 - Ethanol alters the osteogenic differentiation of amniotic fluid-derived stem cells. AU - Hipp, Jennifer A.. AU - Hipp, Jason D.. AU - Atala, Anthony. AU - Soker, Shay. PY - 2010/10/1. Y1 - 2010/10/1. N2 - Background: Fetal alcohol spectrum disorder (FASD) is a set of developmental defects caused by prenatal alcohol exposure. Clinical manifestations of FASD are highly variable and include mental retardation and developmental defects of the heart, kidney, muscle, skeleton, and craniofacial structures. Specific effects of ethanol on fetal cells include induction of apoptosis as well as inhibition of proliferation, differentiation, and migration. This complex set of responses suggests that a bioinformatics approach could clarify some of the pathways involved in these responses. Methods: In this study, the responses of fetal stem cells derived from the amniotic fluid (AFSCs) to treatment with ethanol have been examined. Large-scale transcriptome analysis of ethanol-treated AFSCs ...
In recent years, great interest has been devoted to the use of Induced Pluripotent Stem cells (iPS) for modeling of human genetic diseases, due to the possibility of reprogramming somatic cells of affected patients into pluripotent cells, enabling differentiation into several cell types, and allowing investigations into the molecular mechanisms of the disease. However, the protocol of iPS generation still suffers from technical limitations, showing low efficiency, being expensive and time consuming. Amniotic Fluid Stem cells (AFS) represent a potential alternative novel source of stem cells for modeling of human genetic diseases. In fact, by means of prenatal diagnosis, a number of fetuses affected by chromosomal or Mendelian diseases can be identified, and the amniotic fluid collected for genetic testing can be used, after diagnosis, for the isolation, culture and differentiation of AFS cells. This can provide a useful stem cell model for the investigation of the molecular basis of the diagnosed
Bone tissue engineering strategies require cells with high proliferative and osteogenic potential as well as a suitable scaffold to support the development of these as they form new bone tissue. In this study, we evaluated whether the differentiation stage of amniotic fluid stem cells (AFSC) could enhance the regeneration of critical sized femoral defects in a rat model. For this purpose, AFSC were seeded onto a starch-poly(ε-caprolactone) (SPCL) scaffold and were cultured in vitro in osteogenic culture media for different periods of time in order to obtain: i) undifferentiated cells, ii) cells committed to the osteogenic phenotype and iii) "osteoblast-like" cells. In vitro results indicate that AFSC were considered to be osteogenically committed by the end of week 2 and osteoblastic-like after week 3 in culture. Constructs composed of AFSC-SPCL scaffolds from each differentiation stage were implanted into critical sized femoral defects. The quality of new tissue formed in the defects was ...
Vascular Endothelial Growth Factor Up-Regulation in Human Amniotic Fluid Stem Cell Enhances Nephroprotection after Ischemia-Reperfusion Injury in the Rat The authors evaluated if the up-regulation of vascular endothelial growth factor strengthens the protective effect of amniotic fluid stem cells in a renal ischemia-reperfusion injury model. [Crit Care Med] Abstract Secretome of Human Fetal Mesenchymal Stem Cell Ameliorates Replicative Senescence Researchers hypothesized that the bioactive factors secreted by mesenchymal stem cells (MSC) isolated from human fetal tissues (hFMSC), collectively named as hFMSC secretome, could possess beneficial effects on human adult MSC undergoing replicative senescence, thus promoting their capability of proliferation and differentiation. [Stem Cells Dev] Abstract A Novel High Throughput Screening Platform Reveals an Optimized Cytokine Formulation for Human Hematopoietic Progenitor Cell Expansion Scientists used combinatorial cell culture technology ...
Scientists hailing from the Wake Forest Institute for Regenerative Medicine (WFIRM) have been putting their efforts into a revolutionary approach for helping chronic kidney disease sufferers get a firmer grasp on their situation and repair any tissues that may have been ravaged by the disease. This solution comes in the form of human amniotic fluid-derived stem cells, which the scientists have posited would not only repair tissue, but even help recover kidney function that has been lost. The researchers at WFIRM have been at the cutting edge of stem cell research for quite some time, with their teams also pioneering the equally impressive 3D bioprinting of organs and tissues. According to the scientists, the amniotic fluid-derived stem cells are unique in that they can be utilized as universal cell sources, meaning they can fill in for numerous different types of cells, while simultaneously benefiting the entirety of the organism with anti-inflammatory properties and potential ...
Epithelial and fibroblast cells were obtained from cultures of amniotic fluid cells. Epithelial cells demonstrated high activities of histidase. In contrast, histidase activity was not detected in fibroblasts derived from the same original culture. This observation indicates that cultures of amniotic fluid cells consist of cells with different biochemical properties as well as morphological characteristics. ...
Whether these cells are as versatile as embryonic stem cells remains to be determined," said Atala, "but the current finding is certainly encouraging.". Atala stopped short of calling the cells pluripotent, which means the ability to form many cell types. He said while the cells meet some of the characteristics of pluripotency, such as versatility, they do not form tumors when implanted in animals, which is also considered a characteristic. The fact that the amnion cells are less likely to form tumors may be one advantage that they have over embryonic stem cells in their potential for clinical use.. Co-researchers were Alessandro Valli, Ph.D., Margit Rosner, student, Christiane Fuchs, MSc., Nicol Siegel, MSc., and Helmut Dolznig, Ph.D., from the Medical University of Vienna, Colin E. Bishop, Ph.D., from Wake Forest, and Ulrike Mädel, student, and Wilfried Feichtinger, M.D., from Wunschbaby Zentrum, in Vienna, Austria.. About the Wake Forest Institute for Regenerative ...
Amniotic fluid is concentrated in hyaluronic acid, stem cells, cytokines, and proteins. These important compounds are the fundamental cells existing in the body. Hyaluronic acid is a naturally occurring liquid found in the knee that helps to cushion the knee and can help sooth any joint.. You may have heard about amniotic fluid injections and wondered if this treatment option was right for you and your situation. The best way to find out is to discuss your condition with one of our physicians so you can be evaluated. These are some common conditions that can benefit from amniotic fluid injections:. ...
Abstract: Deficiency of glycosidases is a fundamental feature of the hereditary diseases of glycoconjugate accumulation. The data obtained on activity of glycosidases in cell culture of normal amnionic fluid might be used as standards in prenatal diagnostics of hereditary glycolipidoses and glycoproteinoses. Use of cell culture of amnionic fluid for prenatal diagnosis of Tay-Sachs disease is ...
Restricted Item. Print thesis available in the University of Auckland Library or available through Inter-Library Loan. Three major cell types, termed E, F and AF, constitute cultures of second trimester human amniotic fluids and are widely used for the prenatal diagnosis of inherited disorders of metabolism. Current evidence suggests that the predominant cell type, the AF cells, retain properties of trophoblast and it has been proposed that they are derived from the trophoblast of the placenta. Since the trophoblast is thought to be the site of the aromatization of C19 -steroids in the placenta, it was considered that AF type amniotic fluid cell cultures should possess this steroidogenic activity. Investigations were therefore undertaken to explore this hypothesis. Initial experiments involved culturing AF and F type cells in the presence and absence of dehydroepiandrosterone sulphate and subsequently incubating the cell layers with [4-14C] androstenedione. Isolation and identification of the ...
Tendons are highly prone to injury and the intrinsic hypocellularity and hypovascularity make their natural healing extremely slow and inefficient when severed damaged. Surgical repair with grafts is common but unsuccessful in a long term basis. The development of tissue engineering strategies based on stem cells explores a natural endogenous system of regeneration with potential for tendon application. We propose to establish biochemical culturing conditions to assess the tenogenic potential of human adipose stem cells (hASCs) and amniotic fluid-derived stem cells (hAFSCs), known for their proliferative and differentiation capacities. Since several growth factors (GFs) participate in tendon formation and ECM synthesis, these GFs were added to the culture medium to stimulate tenogenic differentiation of these cells. This study also envisions the application of hASCs and/or hAFSCs in cell-based strategies for tendon repair ...
The usage of stem cells is a promising strategy for the repair of damaged tissue in the injured brain. Recently, amniotic fluid (AF) cells have received a lot of attention as an alternative source of stem cells for cell-based therapies. However, the
In a previous study, we isolated human amniotic fluid (AF)-derived mesenchymal stem cells (AF-MSCs) and utilized normoxic conditioned medium (AF-MSC-norCM) which has been shown to accelerate cutaneous wound healing. Because hypoxia enhances the wound healing function of mesenchymal stem cell-conditioned medium (MSC-CM), it is interesting to explore the mechanism responsible for the enhancement of wound healing function. In this work, hypoxia not only increased the proliferation of AF-MSCs but also maintained their constitutive characteristics (surface marker expression and differentiation potentials). Notably, more paracrine factors, VEGF and TGF-β1, were secreted into hypoxic conditioned medium from AF-MSCs (AF-MSC-hypoCM) compared to AF-MSC-norCM. Moreover, AF-MSC-hypoCM enhanced the proliferation and migration of human dermal fibroblasts in vitro, and wound closure in a skin injury model, as compared to AF-MSC-norCM. However, the enhancement of migration of fibroblasts accelerated by AF-MSC-hypoCM
I am 5 weeks into my first pregnancy and I dont know if Im being paranoid or properly cautious. I dont even know if I can leak amniotic fluid at this stage and as my first appointment with my OB isn...
Abbildung 1: Detection of Oct-4 in the nucleus of a human amniotic fluid cell. A phase contrast picture of an amniotic fluid cell population grown under culture conditions used for routine prenatal diagnosis of genetic mutations is presented. For immunocytochemical analysis of cellular Oct-4 expression, cells were fixed and incubated with anti-Oct-4 antibody. Thereafter, cells were washed, incubated with a biotinylated secondary antibody, washed again, and incubated with ExtrAvidin-Cy3 conjugate. One amniotic fluid cell with the red Oct-4-specific signal in the nucleus is shown ...
The CD117 MicroBead Kit has been developed for the positive selection of human hematopoietic stem and progenitor cells from peripheral blood, cord blood or bone marrow. It has also been used for the isolation of various stem cells from adult tissues, e.g. amniotic fluid stem cells1 and cardiomyocyte progenitors2 . - Belgique
The CD117 MicroBead Kit has been developed for the positive selection of human hematopoietic stem and progenitor cells from peripheral blood, cord blood or bone marrow. It has also been used for the isolation of various stem cells from adult tissues, e.g. amniotic fluid stem cells1 and cardiomyocyte progenitors2 . - 中国
1. Steele MV, Breg VR jr. Chromosome analysis of human amniotic fluid cells. Lancet 1966; 1: 383- 385. 2. Valenti C, Schutta EF, Kehaty T. Cytogenetic diagnosis of Downs syndrome in utero. Am J Med Assoc 1969; 207: 1513. 3. Ondrejčák M. Prenatálna cytogenetická diagnostika z buniek plodovej vody. Lek Obzor 1985; 34(6): 329- 333. 4. von Eggeling F, Freytag M, Fahsold R et al. Rapid detection of trisomy 21 by quantitative PCR. Hum Genet 1993; 91(6): 567- 570. 5. Divane A, Carter NP, Spathas DH et al. Rapid prenatal diagnosis of aneuploidy from unculltured amniotic fluid cells using five - colour fluorescence in situ hybridization. Prenat Diagn 1994; 14(11): 1061- 1069. 6. Pertl B, Yau SC, Sherlock J et al. Rapid molecular method for prenatal detection of Downs syndrome. Lancet 1994; 343: 1197- 1198. 7. Kuo WL, Temjin H, Segraves R et al. Detection of aneuploidy involving chromosomes 13, 18 and 21 by fluorescence in situ hybridization (FISH) to interphase and metaphase amniocytes. Am J Hum ...
Background: Spontaneous ascitic fluid infection (SAI) is an acute infection of ascitic fluid. SAI has three subtypes; SBP, CNNA, MNB. The prototype is Spontaneous bacterial peritonitis (SBP). SBP is a frequent and serious complication in patients with cirrhosis leading to significant mortality and morbidity. Diagnosis of SBP is mainly established by elevated polymorphonuclear leukocyte count and positive ascitic fluid bacterial culture. Methodology: Records of patients with suspected diagnosis of Spontaneous ascitic fluid infection were analyzed in the present study. Patients admitted in the last one year i.e., between January 2014 to December 2014 with ascites secondary to cirrhosis were included. All patients with decompensated cirrhosis had undergone ascitic fluid paracenetesis after admission to the hospital. Ascitic fluid was centrifuged and analyzed for Total protein, Albumin, Total and Differential leukocyte count. Ascitic fluid was cultured by using BacTec method to determine the growth ...
This study showed that term amniotic fluid is a relatively rich source of MSCs having high expansion capacities and features similar to MSCs isolated from other sources. We also showed that harvesting amniotic fluid at term caesarean section deliveries by catheter resulted in much higher collection volumes compared with 10-20 ml volumes that were harvested in previous studies of term or pre-term amniotic fluid [24-26]. We were able to harvest on average about 400 ml of fluid from the 20 samples. Comparing the TAF-MSCs described in this study with MSCs isolated from BM and AD, we show that they share a significantly similar transcriptional profile. Taken together, these results suggest that TAF-MSCs may be an alternative and complimentary source of MSCs with features making them highly attractive for further investigation in in-vivo pre-clinical functional assessments.. There are only a limited number of publications characterizing term human amniotic fluid cells [24-26]. In line with these ...
Question - Diagnosed with severely low amniotic fluid levels. On medication. Will amniotic fluid replenish? . Ask a Doctor about Amniotic fluid, Ask a General & Family Physician
The miRNAs are a new class of nucleic acids that show promise as useful clinical biomarkers for several diseases (6-8). Our data revealed that the expressions of miR-125b-2, miR-155, and miR-3156 in the amniotic fluid culture were significantly higher in pregnant women carrying fetuses with DS than in the control group. The study sample size was small; however, to the best of our knowledge, our study is the first to demonstrate significant differences in miRNA expression in cell cultures between fetuses with and without DS. Many studies have evaluated the association of miRNA expression levels with a variety of pathologic conditions, including preeclampsia, fetal congenital cardiac defects, neural tube defects, fetal growth retardation, and DS (9-15). The miRNAs encoded by hsa 21 (i.e., hsa-miR-99a, let-7c, miR-125b-2, miR-155, and miR-802) have been proven to correlate with the complex and variable phenotypes of DS and were shown to be overexpressed in the heart, frontal cortex, and hippocampus ...
Chang Medium® D was developed for the primary culture of human amniotic fluid cells for use in karyotyping and other antenatal genetic testing. This formula has been optimized for both flask and in situ methodologies. Packaged in single bottles, ready-to-use with no mixing required. For in vitro diagnostic use.. ...
De Coppis team has demonstrated that intravenous transplantation of amniotic fluid stem (AFS) cells enhances the muscle strength and improves the survival rate of the affected animals. This is the first study to demonstrate the functional and stable integration of AFS cells into skeletal muscle, highlighting their value as a cell source for the treatment of muscular dystrophies.. However, the research is still at a relatively early stage as the work has only been carried out in animal models.. Dr Coppi said: "Spinal muscular atrophy is a genetic disease affecting one in 6,000 births. It is currently incurable and in its most severe form children with the condition may not survive long into childhood. Children with a less severe form face the prospect of progressive muscle wasting, loss of mobility and motor function. There is an urgent need for improved treatments.. "We are excited by this potential new approach for regenerating skeletal muscle tissue, but much more research is needed. We now ...
The next, I would be plagued with worry that despite all my efforts at natural induction-sex, acupuncture, membrane stripping-that I would reach 42 weeks and, according to my home birth midwives policy, no longer be able to have the home birth that I wanted. And then I would envision a whole host of unlikely and horrendous birth defects that might be the reason I wasnt going into labor, and also the complete financial disaster that would ensue once we had our complicated hospital birth of our very sickly child on our not-so-great insurance policy.. And then, it happened. I had to jump headfirst into my hospital birth fear and let go of the last shred of control I thought I might have when my midwife said, "This looks like a baby that wants to be born in the hospital.". At a routine non-stress test (used to assess the health of the baby by monitoring its heart rate and amniotic fluid levels) the nurse saw one significant deceleration of our babys heart rate. And my amniotic fluid levels were ...
The next, I would be plagued with worry that despite all my efforts at natural induction-sex, acupuncture, membrane stripping-that I would reach 42 weeks and, according to my home birth midwives policy, no longer be able to have the home birth that I wanted. And then I would envision a whole host of unlikely and horrendous birth defects that might be the reason I wasnt going into labor, and also the complete financial disaster that would ensue once we had our complicated hospital birth of our very sickly child on our not-so-great insurance policy.. And then, it happened. I had to jump headfirst into my hospital birth fear and let go of the last shred of control I thought I might have when my midwife said, "This looks like a baby that wants to be born in the hospital.". At a routine non-stress test (used to assess the health of the baby by monitoring its heart rate and amniotic fluid levels) the nurse saw one significant deceleration of our babys heart rate. And my amniotic fluid levels were ...
After a long wait we had the follow-up ultrasound today. Owen has been a very active boy for a while now which has helped us make it through the wait with much optimism. The ultrasound was at 1:30 and our whole clan was allowed into the ultrasound room. The clan being my mom and dad, Joshs mom and sister and of course Josh and I. As the ultrasound progressed every update was better than the last. First off the amniotic fluid level was right on (high amniotic fluid levels are common with trisomy 18 babies). His choroid plexus cysts had resolved, as well as the calcium deposit on his heart. They were just gone, they said there was a good chance they would resolve, but to see it live in person was nothing short of a miracle for us!!! And finally all of the measurements were done and he came in at 3 lbs. 1oz. and measured within one day of his due date, which we were informed never happens. The measurement was the most important thing to us. The geneticist in Little Rock had told us that babies ...
Looking for Non-parametric statistics? Find out information about Non-parametric statistics. Any method of inference that does not depend on the characteristics of the population from which the samples are obtained Explanation of Non-parametric statistics
Health, ...In a breakthrough that may help fill a critical need in stem cell rese...The Mount Sinai researchers found that when compared to cultured adult... There remains today a need in stem cell research for an easily reprog...Amniotic fluid skin cells can be safely obtained from pregnant women u...,Amniotic,fluid,cells,more,efficiently,reprogrammed,to,pluripotency,than,adult,cells,medicine,medical news today,latest medical news,medical newsletters,current medical news,latest medicine news
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Intra-amniotic infections are associated with an increased risk of preterm delivery, which, in turn, may be associated with neurological sequelae in former preterm infants [80]. Microbial presence in the amniotic fluid may elicit maternal and fetal inflammatory response that are then responsible for neonatal complications. The association between elevated inflammatory cytokines IL-1β and IL-6 in the amniotic fluid and subsequent white matter injury has been noted in preterm infants [81]. Elevated levels of inflammatory cytokines in the cord blood including IL-1β, IL-6 IL-8, and TNF-α have also been shown to correlate with neonatal cerebral lesions as detected by MRI after parturition in human premature infants [82]. Furthermore, clinical evidence shows that elevated inflammatory response in the perinatal period has been demonstrated to correlate with long-term neonatal morbidities including cerebral palsy [83], psychomotor deficits [8], and non-neurological diseases including necrotizing ...
Definition : Immunoassay reagents intended to perform quantitative analyses on amniotic fluid samples obtained from pregnant women to determine the ratio of phospholipids (i.e., surfactants) to albumin. These tests are typically used to estimate fetal lung maturity and to assess the associated risk of respiratory distress syndrome if birth occurs. The predictive values are less than 40mg/g for immature lungs and more than 70mg/g for mature lungs, but values may change as pregnancy progresses.. Entry Terms : "Reagents, Immunoassay, Pregnancy, Phospholipid/Albumin Ratio". UMDC code : 20669 ...
Objective: RANTES (regulated on activation, normal T cell expressed and secreted), a potent and versatile chemokine, is capable of attracting monocytes, lymphocytes, basophils, and eosinophils. This cytokine has been implicated in the regulation of the inflammatory response and in the recruitment of macrophages to the implantation site in early pregnancy. RANTES messenger ribonucleic acid and protein have been detected in fetal tissue and first-trimester trophoblast in response to bacterial endotoxin. The purpose of this study was to determine whether intrauterine infection, parturition (preterm and term), and gestational age affect the amniotic fluid concentrations of RANTES in human pregnancy. Study Design: A cross-sectional study was designed to examine the relationship between labor, microbial invasion of the amniotic cavity, gestational age, and RANTES expression in amniotic fluid. Amniotic fluid was obtained from 214 women in the following groups: (1) midtrimester (n = 22), (2) preterm ...
Our findings confirm the hypothesis that medical induction of labour is related to an increased risk of AFE," added Dr. Kramer. "Although the absolute risk increase of AFE for women is very small (four or five total cases and one or two fatal cases per 100,000 women induced) and is unlikely to affect the decision to induce labour in the presence of compelling clinical indications, women and physicians should be aware of this risk if the decision is elective." ...
Our findings confirm the hypothesis that medical induction of labour is related to an increased risk of AFE," added Dr. Kramer. "Although the absolute risk increase of AFE for women is very small (four or five total cases and one or two fatal cases per 100,000 women induced) and is unlikely to affect the decision to induce labour in the presence of compelling clinical indications, women and physicians should be aware of this risk if the decision is elective ...
Scientist have identified a link between exposure to high levels of oestrogen sex hormones in the womb and the likelihood of developing autism. The discovery adds further evidence to support the prenatal sex steroid theory of autism first proposed 20 years ago.. In 2015, a team of scientists at the University of Cambridge and the State Serum Institute in Denmark measured the levels of four prenatal steroid hormones, including two known as androgens, in the amniotic fluid in the womb and discovered that they were higher in male foetuses who later developed autism. These androgens are produced in higher quantities in male than in female foetuses on average, so might also explain why autism occurs more often in boys. They are also known to masculinise parts of the brain, and to have effects on the number of connections between brain cells.. Today, the same scientists have built on their previous findings by testing the amniotic fluid samples from the same 98 individuals sampled from the Danish ...
The present study investigated: (a) the presence of antiphospholipid antibodies and (b) the obstetric outcome in healthy pregnant women showing false-positive TORCH-Toxoplasmosis, Other: syphilis, varicella-zoster, Rubella, Cytomegalovirus (CMV), and Herpes infections-results. Data from 23 singleton healthy pregnancies with false-positive TORCH results were collected. Each woman was systematically screened for TORCH IgG and IgM during the pre-conception assessment and/or at the beginning of pregnancy. In the presence of IgM positivity, when indicated (CMV, toxoplasmosis, rubella, herpes simplex virus), IgG avidity was evaluated and, if possible, polymerase chain reaction was performed on an amniotic fluid sample in order to distinguish between primary infection or false positivity ...
Not only does death linger on as a memory in the nervous system, but suicides will often choose a method that reflects the prototype of their birth experiences. So, those strangled on the umbilical cord may hang themselves, or an overdose of pills is chosen by someone who was drugged at birth. Why? Because of the prototype; for a neonate strangling on the cord, further strangling would have ended the agony. Those drowning in amniotic fluid at birth will opt for death by drowning. Case in point: the writer-actor Spalding Gray had a lifetime obsession with drowning. He used to swim in the ocean as far out as he possibly could until the point of exhaustion and then struggled to try and make it back. He killed himself by jumping off the Staten Island Ferry in the middle of the night. I hypothesize that he may have been drowning at birth and the end of the original sequence would be drowning. In other words, I think he died like he was born. This is never to minimize the pain in the present; ...
Not only does death linger on as a memory in the nervous system, but suicides will often choose a method that reflects the prototype of their birth experiences. So, those strangled on the umbilical cord may hang themselves, or an overdose of pills is chosen by someone who was drugged at birth. Why? Because of the prototype; for a neonate strangling on the cord, further strangling would have ended the agony. Those drowning in amniotic fluid at birth will opt for death by drowning. Case in point: the writer-actor Spalding Gray had a lifetime obsession with drowning. He used to swim in the ocean as far out as he possibly could until the point of exhaustion and then struggled to try and make it back. He killed himself by jumping off the Staten Island Ferry in the middle of the night. I hypothesize that he may have been drowning at birth and the end of the original sequence would be drowning. In other words, I think he died like he was born. This is never to minimize the pain in the present; ...
We just had our 20 week anatomy scan today, and for the most part things looked good with the exception of some renal abnormalities. DH was born with whats called vesiculoureteral reflux and had to have surgery as a baby to reattach his ureters to his bladder. Based on todays scan, we think that may be whats going on with our first LO. She has dilated collecting systems, normal kidneys,, normal bladder, and normal amniotic fluid levels. We are being referred to the Pediatric Nephrologist (who DH and I both work with and adore), as well as a Pediatric Urologist (not even a part of our hospital). Well be getting ultrasounds every 4 weeks now, with both the MFM and Nephrologists there at those appointments. I feel like we have a plan, and Im only slightly having alarm bells go off. I hope this is what DH had - as DH has normal kidney function as an adult. Does anyone have any experience with kidney issues in particular, or with getting a vague diagnosis like this and having to increase ...
An automated analytical instrument for conducting assays for components of interest in fluid samples. The instrument includes a testing system for assaying a fluid sample for a component of interest, a fluid dispensing assembly, an assembly for providing an assay module to the testing system and a fluid sample holding tray transport assembly for transporting a fluid sample holding tray carrying fluid sample holding containers to the fluid dispensing assembly and away from the fluid dispensing assembly. The transport assembly includes a conveyor, a fluid sample holding tray loading unit and a fluid sample holding tray unloading unit. The conveyor includes a carriage which is slidably mounted on a rail and moved along the rail by an endless belt which is fixedly attached to the carriage and driven by a reversible motor. In the operation of the instrument, one or more fluid sample holding trays are placed onto the fluid sample holding tray loading unit. Each fluid sample holding tray so loaded on to the
After a short nap while Joe and Grandpa took Brendan for a walk, we left Brendan in the care of his grandparents as we left for my ultrasound appointment. As we were well aware, the ultrasound was scheduled in order to determine if the baby was actually measuring the size she was supposed to be and to see if the amniotic fluid was low. So it wasnt a complete shock when the ultrasound tech stated that the baby was, in fact, average size for a 37 week fetus and not a 39 week fetus. And then proceeded to show us that there was very little fluid for measurement, leaving me with whats called a low amniotic fluid index. As a labor and delivery nurse I knew the implications of the ultrasounds results and while an AFI of 5 (normal of 10-25) is not extremely low, its low enough to warrant some concern. We were told to wait while the tech paged the doctor and I turned to Joe and explained that one of two things was likely to happen. I would either need to go to the maternity unit for monitoring (to ...
A proper canine neonatal assistance, required to reduce the high perinatal loss rate, imply a full knowledge about the fetal-to-neonatal physiology. Because fetal fluids play an important role throughout mammals pregnancy, influencing fetal growth and development, fetal well being, and contributing to guarantee the most suitable environment for the fetus, the knowledge about fetal fluids biochemical composition is of major importance. At first, the biochemical composition of fetal fluids collected by normal developed, healthy and viable newborns, is necessary to depict the normal features, and represent the first step for the further detection of abnormalities associated to fetal/neonatal distress and useful for the early identification of newborns needing special attention, immediately after birth ...
Ponevadz jsem se posledni dobou zajimal o NFS pod Linuxem (u portmapu v RedHatu se pise ye tam jsou odstraneny zname chyby NFS a NIS) narazil jsem na AFS. Vsude se totiz pise ze NFS neni moc bezbecne. Muze min nejakz odbornik rici jak je to s bezpecnosti u AFS? Myslim ze ve verzi 2.2 jiz bude defaultne ve zdrojacich jadra. Pokud se nemylim AFS ma jiz RedHat 5.1 Mohl by tedy nekdo zhodnotit vyhody a nevyhody AFS a NFS P.S.: Mimochodem nevite proc v RedHatu neni PCNFS daemon a jak je to s Debianem? P.S.S: Jaky je rozdil mezi PCNFS a BWNFS (zajima mne hlavne bezpecnost ...
The principle behind salt or sugar preservation is to A) introduce a mechanical barrier to microbial invasion. B) reduce water activity (aw). C) introduce a microbicide in anticipation of contaminating bacteria or fungi. D) accomplish all of the described functions depending on the food being preserved.
Apply with brush to cover entire screen with screen filler; when the screen filler is dry, the drawing fluid can be washed out, allowing for the area to be printed.
A device for transporting a plurality of similarly sized beverage cans includes a transparent, flexible pouch of fixed dimensions, and a rigid housing in which the pouch is slidably received. The front and rear walls of the pouch and housing are spaced apart a distance about equal to the length of the cans, so that when the cans are inserted with their ends adjacent the front and rear walls, they will remain in unidirectional alignment upon removal of the pouch from the housing. The pouch includes a flap for keeping the cans in the pouch, and adhesive tape for securing the flap to the front of the pouch. The tape passes through a facial opening in the housing in order to seal the pouch while it is in the housing. The pouch is preferably transparent so that the number of cans can be accurately counted, and so that it can be visually confirmed that all of the cans are of the type returnable for a refund of any deposit. In one preferred embodiment the housing and pouch walls serve as the sole means for
Current treatments for tendon injuries often fail to fully restore joint biomechanics leading to the recurrence of symptoms, and thus resulting in a significant health problem with a relevant social impact worldwide. Cell-based approaches involving the use of stem cells might enable tailoring a successful tendon regeneration outcome. As growth factors (GFs) powerfully regulate the cell biological response, their exogenous addition can further stimulate stem cells into the tenogenic lineage, which might eventually depend on stem cells source. In the present study we investigate the tenogenic differentiation potential of human- amniotic fluid stem cells (hAFSCs) and adipose-derived stem cells (hASCs) with several GFs associated to tendon development and healing; namely, EGF, bFGF, PDGF-BB and TGF-β1. Stem cells response to biochemical stimuli was studied by screening of tendon-related genes (collagen type I, III, decorin, tenascin C and scleraxis) and proteins found in tendon extracellular matrix (ECM)
Amniotic fluid[edit]. It is also possible to extract stem cells from amniotic fluid for both autologous or heterologous use at ... Severe liver injury can result from hepatic veno-occlusive disease (VOD). Elevated levels of bilirubin, hepatomegaly and fluid ...
Beall MH; van den Wijngaard JPHM; van Gemert MJC; Ross MG (2007). "Amniotic Fluid Water Dynamics". Placenta. 28: 816-23. doi: ... from food and fluids) to 2,300 mL, or approximately 1,850 mL/ day from fluids alone Proper nutrition is important after ... approximately 2,400 mL/day from fluids. Fetal alcohol spectrum disorder Prenatal nutrition Smoking and pregnancy Barasi EM ( ...
A small proportion of cases are due to the embolization of air, fat, or amniotic fluid. Diagnosis is based on signs and ... Pantaleo, G; Luigi, N; Federica, T; Paola, S; Margherita, N; Tahir, M (2014). "Amniotic fluid embolism: review". Current ...
1967-1981 Developed a new method for measuring the bilirubin content of amniotic fluid. In collaboration with the Department of ... "Determination of bilirubin in amniotic fluid. A new, simple, and efficient method". Clinical Chemistry. 16 (3): 180-4. PMID ... 1973 "Human Uterine Fluid Potassium and the Menstrual Cycle." J Obstet Gynaecol Br Commonw. Jun;80(6):553-61. Member of the ... Clemetson CA, Verma UL, De Carlo SJ (March 1977). "Secretion and reabsorption of uterine luminal fluid in rats". Journal of ...
1974 Sep;15(9):743-9. " "Amniotic fluid indicators of fetal maturity. Enlander D. Obstet Gynecol. 1972 Oct;40(4):605-6. " " ...
For prenatal samples (only): if the amniotic fluid (non-confluent culture cells) are provided. Amniotic fluid is added and ... However, their kidneys cannot keep up, excess sugar is excreted into their urine, and this drag along fluids from the ... As a diabetic individual drinks more fluids to satisfy their thirst, he or she urinates even more. Dehydration Effected areas ...
The fetus urinates hourly and produces most of the amniotic fluid in the second and third trimester of pregnancy. The amniotic ... "Amniotic Fluid: Not Just Fetal Urine Anymore". Journal of Perinatology. 25 (5): 341-348. doi:10.1038/sj.jp.7211290. PMID ... A plot of bladder (intravesical) pressure against the depressant of fluid in the bladder (called a cystometrogram), will show a ... "Chemical Characterization of Territorial Marking Fluid of Male Bengal Tiger, Panthera tigris". Journal of Chemical Ecology. 34 ...
Stem cells have been identified in amniotic fluid. As many as 1 in 100 cells collected during amniocentesis has been shown to ...
Amniotic fluid can also be extracted and analysed. A wide variety of biomarkers may be studied to detect other diseases. For ... A liquid biopsy, also known as fluid biopsy or fluid phase biopsy, is the sampling and analysis of non-solid biological tissue ... When studying the central nervous system, cerebrospinal fluid may be sampled instead of blood. Unlike traditional biopsies, ... "Cerebrospinal Fluid Biomarker and Brain Biopsy Findings in Idiopathic Normal Pressure Hydrocephalus". PLoS ONE. 9 (3): e91974. ...
Sometimes, excess amniotic fluid or polyhydramnios is exhibited. Edwards syndrome is a chromosomal abnormality characterized by ... The most common intracranial anomaly is the presence of choroid plexus cysts, which are pockets of fluid on the brain. These ...
Fetuses drink amniotic fluid and urinate into it. The sense modalities of the fetus develop prenatally and are functioning very ... Breath movements can be seen from the 19th week on, with the fetus taking amniotic fluid into his lungs. Eye movements are ...
An amniotic fluid sample is collected via amniocentesis and the sample is spun down in a centrifuge at 1000 rpm for 3-5 minutes ... The lecithin-sphingomyelin ratio (a.k.a. L-S or L/S ratio) is a test of fetal amniotic fluid to assess for fetal lung ... As such, if a sample of amniotic fluid has a higher ratio, it indicates that there is more surfactant in the lungs and the baby ... Phosphatidylglycerol - another amniotic fluid marker of fetal lung maturity Surfactant-albumin ratio Lamellar body count Foam ...
At week 11, the fetus can open its mouth and suck its fingers; at week 12, it begins to swallow amniotic fluid. In addition to ...
Amniotic fluid embolism - embolism of amniotic fluid, foetal cells, hair, or other debris that enters the mother's bloodstream ... Amniotic-fluid embolism is a rare complication of childbirth. Paradoxical embolism Pulmonary embolism Kumar V.; Abbas A.K.; ...
Amniotic stem cells are a topic of active research. Use of stem cells from amniotic fluid overcomes the ethical objections to ... Multipotent stem cells are also found in amniotic fluid. These stem cells are very active, expand extensively without feeders ... called amniotic stem cells "the future of medicine". It is possible to collect amniotic stem cells for donors or for ... "Biocell Center opens amniotic stem cell bank in Medford". Mass High Tech Business News. 2009-10-23. Retrieved 2012-08-26. "News ...
Sugai T, Miyahara A: Leprosy bacilli in amniotic fluid. Igaku Chuoshi, 1915. Sugai T, Kawabata K: Fate of leprosy bacilli in ...
... a passionate couple encased in an amniotic fluid bubble; and a man inside of a red fruit staring at a mouse in a transparent ...
... are found in amniotic fluid, follicular fluid and seminal plasma of the reproductive system. These glycoproteins have distinct ... Bell SC, Hales MW, Patel SR, Kirwan PH, Drife JO, Milford-Ward A (Sep 1986). "Amniotic fluid concentrations of secreted ... Joshi SG, Smith RA, Stokes DK (Nov 1980). "A progestagen-dependent endometrial protein in human amniotic fluid". Journal of ...
After birth, the smell of amniotic fluid (which was previously disgusting) becomes attractive for ewes. Amniotic fluid is one ... Newborns are initially attracted to their own amniotic fluid because that odor is familiar. Although exposure to amniotic fluid ... allow her to be attracted to any newborn lamb associated with that amniotic fluid. The amniotic fluid produces olfactory cues, ... For example, babies would more often suck from a breast treated with an amount of their own amniotic fluid, rather than the ...
Hitti J, Hillier SL, Agnew KJ, Krohn MA, Reisner DP, Eschenbach DA (2001). "Vaginal indicators of amniotic fluid infection in ... In many studies, F. nucleatum cells have been isolated from the amniotic fluid, placenta, and chorioamnionic membranes of women ...
A 1927 study demonstrated the presence of bacteria in the amniotic fluid of those that were in labor for longer than six hours ... While bacteria are often found in the amniotic fluid of failed pregnancies, they are also found in particulate matter that is ... Hitti J, Hillier SL, Agnew KJ, Krohn MA, Reisner DP, Eschenbach DA (2001). "Vaginal indicators of amniotic fluid infection in ...
N Z Med J. 1958 Oct;57(321):470-7. Foetal renal hypoplasia and the origin of amniotic fluid. Green GH. J Obstet Gynaecol Br Emp ...
Chronic polyhydramnios where excess amniotic fluid accumulates gradually Acute polyhydramnios where excess amniotic fluid ... is a medical condition describing an excess of amniotic fluid in the amniotic sac. It is seen in about 1% of pregnancies. It is ... Amniotic fluid index Alexander, ES, Spitz, HB, Clark, RA. Sonography of polyhydramnios. AJR Am J Roentgenol 1982; 138:343 Hill ... a deficiency in amniotic fluid. In most cases, the exact cause cannot be identified. A single case may have one or more causes ...
AFP in amniotic fluid has one or two sources. The fetus normally excretes AFP into its urine, hence into the amniotic fluid. A ... Abnormally elevated AFP in amniotic fluid can have one or more of many different causes: normal elevation. 75% of AF AFP test ... There are two categories of AFP tests: tests performed on serum (blood plasma), and tests performed on amniotic fluid. Tests ... measurement of AFP in amniotic fluid obtained via amniocentesis. Maternal serum AFP (MSAFP) varies by orders of magnitude ...
... the tissues die and are absorbed into the amniotic fluid. A baby with congenital amputation can be missing a portion of a limb ... Fibrous bands from the ruptured amnion float in the amniotic fluid and can get entangled with the fetus, thus reducing blood ... It is known to be caused by blood clots forming in the fetus while in utero (vascular insult) and from amniotic band syndrome: ... One common cause is amniotic band syndrome, which occurs when the inner fetal membrane (amnion) ruptures without injury to the ...
... inflammatory medications in the third trimester and adverse effects to the developing fetus including too little amniotic fluid ...
Amniotic fluid embolism (AFE) is a pregnancy complication that causes life-threatening conditions, such as heart failure. Learn ... Amniotic fluid embolism. Amniotic fluid embolism (AFE), also known as anaphylactoid syndrome of pregnancy, is a pregnancy ... It happens when amniotic fluid (the fluid surrounding your unborn child) or fetal cells, hair, or other debris make their way ... In rare cases, it can happen during an abortion or while having a small sample of amniotic fluid taken for examination ( ...
... characteristically referred to as Amniotic fluid embolism (AFE). The underlying mechanism for AFE is still poorly understood. ... The disastrous entry of amniotic fluid into the maternal circulation leads to dramatic sequelae of clinical events, ... Amniotic fluid embolismObstet and Gynecol C1 N AmYear: 19992643544. 9. Gei G,Hankins GD. Amniotic fluid embolism: An ... United kingdom amniotic fluid embolism registerBJ0GYear: 200511216259. 7. Morgan M. Amniotic fluid embolismAnesthesiaYear: ...
A fatal case of amniotic fluid embolism in a Coloured multipara with toxaemia and placenta praevia, which resulted in an intra- ... Keyword(s) : Amniotic fluid embolism, Case report, Coloured multipara, Obstetrics and gynaecology, Pathology and Placenta ... A fatal case of amniotic fluid embolism in a Coloured multipara with toxaemia and placenta praevia, which resulted in an intra- ... oa South African Medical Journal - Amnionvog-embolisme (amniotic fluid embolism) - 'n bewese geval en 'n kort ...
Awad IT, Shorten GD: Amniotic fluid embolism and isolated coagulopathy: Atypical presentation of amniotic fluid embolism. Eur J ... or any other breach of the barrier between maternal blood and amniotic fluid allows the forced entry of amniotic fluid into the ... Case Scenario: Amniotic Fluid Embolism Laura S. Dean, M.D.; Raford P. Rogers, M.D.; Russell A. Harley, M.D.; David D. Hood, M.D ... Microscopically, amniotic fluid debris may be found in other organs.30 - 32 Hankins et al . showed that much of observable ...
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7th Management of OB Crisis 2 of 6 This discussion includes the presentation of amniotic fluid embolism (AFE), the ... This discussion includes the presentation of amniotic fluid embolism (AFE), the pathophysiology of AFE and immediate supportive ... The proposed diagnostic criteria for amniotic fluid embolism for research reporting is also discussed. ...
"Amniotic fluid embolism: analysis of the national registry". Am J Obstet Gynecol. vol. 172. 1995. pp. 1158-67. ... Davies, S. "Amniotic fluid embolus: a review of the literature". Can J Anesth. vol. 48. 2001. pp. 88-98. ... Conde-Agudelo, A, Romero, R. "Amniotic fluid embolism: an evidence-based review". Am J Obstet Gynecol. vol. 201. 2009. pp. e441 ... "Amniotic fluid embolism: diagnosis and management". Am J Obstet Gynecol. vol. 215. 2016. pp. B16-24. ...
Ochiai, D, Masuda, H, Abe, Y, Otani, T, Fukutake, M, Matsumoto, T, Miyakoshi, K & Tanaka, M 2018, Human Amniotic Fluid Stem ... Ochiai, D., Masuda, H., Abe, Y., Otani, T., Fukutake, M., Matsumoto, T., ... Tanaka, M. (2018). Human Amniotic Fluid Stem Cells ... Human Amniotic Fluid Stem Cells: Therapeutic Potential for Perinatal Patients with Intractable Neurological Disease. The Keio ... Among MSCs, amniotic fluid stem cells (AFS) have a number of characteristics that make them attractive candidates for tissue ...
We present a case of amniotic fluid embolism which is unusual in its presentatio ... A case of amniotic fluid embolism in a twin pregnancy in the second trimester.: ... A case of amniotic fluid embolism in a twin pregnancy in the second trimester.. Authors * Kelly, M C ... We present a case of amniotic fluid embolism which is unusual in its presentation in the second trimester of a twin pregnancy, ...
Amniotic Fluid Embolism (AFE) is rare, but associated with high mortality and as well morbidity rates for the binomial maternal ... Although mortality due to Amniotic Fluid Embolism has decreased in recent surveys, it still remains among the most important ... Keywords Embolism, Amniotic Fluid; Maternal Mortality; Hypertension, Pulmonary; Heart Failure; Disseminated Intravascular ... because there is still not any laboratory or imaging method able to confirm the Amniotic fluid embolism. After initial ...
... the amniotic membrane is ruptured so as to permit free flow of the amniotic fluid from the uterus. The amniotic fluid is ... Removal of the amniotic fluid reduces if not eliminates the risk of amniotic fluid embolism (AFE), probably the most dangerous ... This prevents the patient from experiencing an amniotic fluid embolism, in which the amniotic fluid can enter the bloodstream ... Release of the amniotic fluid allows the uterus to contract and become firm, reducing the risk of perforation of the uterus ...
Amniotic fluid embolism.. Br Med J 1970; 1 doi: https://doi.org/10.1136/bmj.1.5695.545 (Published 28 February 1970) Cite this ...
Amniotic fluid is a clear, slightly yellowish liquid that surrounds the unborn baby (fetus) during pregnancy. It is contained ... While in the womb, the baby floats in the amniotic fluid. The amount of amniotic fluid is greatest at about 34 weeks (gestation ... The amniotic fluid constantly moves (circulates) as the baby swallows and "inhales" the fluid, and then releases it. ... Amniotic fluid disorders. In: Gabbe SG, Niebyl JR, Simpson JL, et al, eds. Obstetrics: Normal and Problem Pregnancies. 7th ed. ...
These include having too much or too little amniotic fluid, and what happens if fluid leaks. ... This article looks at what amniotic fluid is, its functions, and some disorders. ... Amniotic fluid is the fluid that surrounds and protects an embryo while it is growing in the uterus. It is essential for fetal ... Stem Cells From Amniotic Fluid It is possible to take stem cells from amniotic fluid and reprogram them to a more versatile ...
Researchers have found that some stem cells in human amniotic fluid appear to have many of the key therapeutic benefits of ... Amniotic-fluid stem cells, which are sloughed off by the fetus, are "a different kind of a stem cell," Atala said. "Its not as ... Amniotic-fluid stem cells lie somewhere between the two major categories of stem cells: embryonic and adult. ... Researchers have found that some stem cells in human amniotic fluid appear to have many of the key therapeutic benefits of ...
When amniotic fluid is released through the cervix into the vagina, a quantity thereof may pass into the housing and sensed by ... The device comprises a housing manufactured from biocompatible material having a hole through which amniotic fluid in the ... by which a female patient or her health caregiver is notified of the rupture of membranes by the release of amniotic fluid. ... When amniotic fluid is discharged from the uterus into the vagina (the water breaks), a portion of the fluid flows through the ...
... you might be wondering if youre leaking amniotic fluid or if its something else. The level of amniotic fluid you retain will ... Heres how to tell if youre leaking amniotic fluid, your risk factors, and when to call your doctor. ... Vaginal fluid is usually white or yellow in color. Another way you can try to determine if the fluid is amniotic fluid is to ... Risk factors for amniotic fluid leakage. Leaking amniotic fluid can be dangerous for you and your baby at any point during your ...
Find breaking news, commentary, and archival information about Amniotic Fluid From The tribunedigital-chicagotribune ... Amniotic fluid surrounding unborn babies in the womb contains surfactant, a chemical critical for lung function in newborns. ... Doctors at the clinic said the death was the result of an amniotic fluid embolism, a rare occurrence in which a bubble of ... Doctors at the clinic said the death was the result of an amniotic fluid embolism, a rare occurrence in which a bubble of ...
New interest in amniotic fluid-derived cells was initiated by two independent findings. In 2001, it was suggested that amniotic ... C. M. Gosden, "Amniotic fluid cell types and culture," British Medical Bulletin, vol. 39, no. 4, pp. 348-354, 1983. View at ... Amniotic Fluid Stem Cells: Future Perspectives. Margit Rosner,1 Katharina Schipany,1 Bharanidharan Shanmugasundaram,1,2 Gert ... Today, amniotic fluid stem (AFS) cells are widely accepted as a new powerful tool for basic research as well as for the ...
Find out more what it means when youre leaking amniotic fluid during pregnancy (also called premature rupture of membranes) ... Causes of leaking amniotic fluid. In most cases of leaking amniotic fluid, the rupture of your amniotic sac is just a prelude ... What should you do if youre leaking amniotic fluid?. If you think youre leaking amniotic fluid at any point during your ... Symptoms of leaking amniotic fluid. The main symptom of PROM to watch out for is the leaking or gushing of fluid from the ...
... connected with amniotic fluid is amniotic fluid embolism. Recent studies show that amniotic fluid contains a considerable ... Amniotic fluid, also known as Camerons fluid is present from the formation of the gestational sac. Amniotic fluid is in the ... The amniotic fluid is the protective liquid contained by the amniotic sac of a gravid Amniote. This fluid serves as a cushion ... Swallowed amniotic fluid creates urine and contributes to the formation of meconium. Amniotic fluid protects the developing ...
Amniotic fluid is present from the formation of the gestational sac. Amniotic fluid is in the amniotic sac. It is generated ... The amniotic fluid is the protective liquid contained by the amniotic sac of a gravid amniote. This fluid serves as a cushion ... Complications related to amniotic fluidEdit. Too little amniotic fluid (oligohydramnios) can be a cause or an indicator of ... Swallowed amniotic fluid creates urine and contributes to the formation of meconium. Amniotic fluid protects the developing ...
This test is intended for amniotic fluid samples that does not have any accompanying cytogenetic test order (e.g. chromosome ...
Amniotic fluid index (AFI) is a quantitative estimate of amniotic fluid and an indicator of fetal well-being. It is a part of ... and it can lead to low levels of amniotic fluid. Maternal complications Maternal complications may cause low amniotic fluid. ... www.fetalanomalies.org/Fluid.html Griffin M, Attilakos G, Greenwood R, Denbow M (2009). "Amniotic fluid index in low-risk, post ... AFI is the score (expressed in cm) given to the amount of amniotic fluid seen on ultrasonography of a pregnant uterus. To ...
Amniotic fluid not only protects the fetus from injury and temperature changes, it also is circulated by the fetus every 3 ...
  • If various neonatal conditions, including birth asphyxia, preterm birth, and congenital abnormalities, which result in long-lasting severe impairments, could be predicted during pregnancy, it would allow collection of small samples of amniotic fluid cells by amniocentesis. (elsevier.com)
  • In rare cases, it can happen during an abortion or while having a small sample of amniotic fluid taken for examination ( amniocentesis ). (healthline.com)
  • It happens when amniotic fluid (the fluid surrounding your unborn child) or fetal cells, hair, or other debris make their way into your blood. (healthline.com)
  • If the placenta is not providing enough blood and nutrients to the baby, then the baby may stop recycling fluid. (wikipedia.org)
  • The amniotic fluid and other material enters the mother's bloodstream, most likely due to small tears in the lower part of the uterus, the part of the cervix that forms a canal connecting the vagina to the uterus (endocervix), or because of damage or abnormality affecting the placenta. (rarediseases.org)
  • Human amniotic membrane (HAM) consists of 2 conjoined layers, the amnion and chorion, and forms the innermost lining of the amniotic sac or placenta. (wellmark.com)
  • When the amniotic sac ruptures, large amounts of fluid leaving the uterus may increase the risk of placental abruption (early detachment of the placenta) or umbilical cord prolapse (when the cord falls down through the cervical opening) where it may be compressed. (chop.edu)
  • The total volume of water during the second trimester (including the fluid in baby, placenta and the amniotic fluid) can reach up to five litres. (consumerhealthdigest.com)
  • To determine whether the placenta also releases stress hormones, the researchers compared the cortisol level in the mother's saliva with the CRH level in the amniotic fluid - and determined that there was no connection: "The baby obviously remains protected against negative effects in case of acute, short-term stress to the mother," Ehlert concludes. (healthcanal.com)
  • If the doctor diagnoses a problem in your placenta, yours and your baby's health will be monitored and you will have to undergo regular scans to check the amniotic fluid levels (4) . (momjunction.com)
  • Immunoregulatory effects on T lymphocytes by human mesenchymal stromal cells isolated from bone marrow, amniotic fluid, and placenta. (sigmaaldrich.com)
  • In this study, MSCs isolated from amniotic fluid and placenta were compared with bone marrow MSCs. (sigmaaldrich.com)
  • Amniotic fluid is produced by the ___ of fluid across the placenta prior to 19 weeks. (brainscape.com)
  • Since the trophoblast is thought to be the site of the aromatization of C19 -steroids in the placenta, it was considered that AF type amniotic fluid cell cultures should possess this steroidogenic activity. (openthesis.org)
  • These adult-type stem cells from amniotic fluid and placenta (afterbirth) have all the positives scientists claim to want from stem cells-flexibility to make different tissues, easy and long-term growth in the lab, no tumors-without the negatives associated with embryonic stem cells. (texasrighttolife.com)
  • This same amniotic fluid is then swallowed by the baby and re-absorbed by the lining of the placenta. (scienceandsensibility.org)
  • If the mother's placenta is not acting sufficiently anymore, this may lead to a decrease in amniotic fluid. (scienceandsensibility.org)
  • A fatal case of amniotic fluid embolism in a Coloured multipara with toxaemia and placenta praevia, which resulted in an intra-uterine death and postpartum bleeding, is reported. (journals.co.za)
  • As higher animals evolved from reptiles, the pattern of giving birth shifted from laying eggs to producing live offspring, and the system within evolved to produce what can be considered a modified egg, amniotic fluid, umbilical cord, and the placenta. (curejoy.com)
  • As pregnancy progresses, the placenta takes on the primary nutritive role, and the amniotic fluid supplements this with additional proteins. (curejoy.com)
  • For the first time, scientists have demonstrated that stem cells found in amniotic fluid meet an important test of potential to become specialized cell types, which suggests they may be useful for treating a wider array of diseases and conditions than scientists originally thought. (innovations-report.com)
  • Nanowerk News ) Researchers have demonstrated the feasibility of focused fetal gene expression analysis of target genes found in amniotic fluid using Standardized NanoArray PCR (SNAP) technology. (nanowerk.com)
  • ICD-9 code 673.1 for Amniotic fluid embolism is a medical classification as listed by WHO under the range -COMPLICATIONS OF THE PUERPERIUM (670-677). (aapc.com)
  • That's because when you're upright, your baby's head blocks the opening and keeps most amniotic fluid trapped inside. (whattoexpect.com)
  • The amniotic fluid is part of the baby's life support system . (americanpregnancy.org)
  • The fluid contains cells that provide important information about your unborn baby's health. (medlineplus.gov)
  • That extra fluid could be used to isolate fetal stem cells from the baby, as it contains a varied population of cells that originate from the tissues of the baby's skin, respiratory, digestive and urinary tracts. (parentsguidecordblood.org)
  • Preserving and storing your baby's amniotic fluid stem cells could potentially offer your child, and other members of your immediate family, an opportunity to benefit from these advances in medical treatments. (healthywomen.org)
  • Does amniotic fluid enter the baby's body while in the womb? (ndtv.com)
  • Home » Frequently asked Questions on Health » Does amniotic fluid enter the baby's body while in the womb? (ndtv.com)
  • Of course the amniotic fluid enters the nose and all the other orifices of the baby's body. (ndtv.com)
  • Amniotic fluid protects the developing baby by cushioning against blows to the mother's abdomen, allowing for easier fetal movement and promoting muscular/skeletal development. (wikipedia.org)
  • In this disorder, it is hypothesized that a pregnant woman has a severe, allergic reaction to amniotic fluid or other fetal material such as fetal cells, which enter the mother's bloodstream. (rarediseases.org)
  • Researchers and physicians do not fully understand why amniotic fluid or fetal cells entering the mother's bloodstream causes this reaction in some women. (rarediseases.org)
  • Within a very short period the sac fills with amniotic fluid that is made up of liquid from the mother's circulatory system. (gynob.com)
  • Amniotic fluid helps protect the baby from trauma to the mother's abdomen. (scienceandsensibility.org)
  • Women are more likely to be diagnosed with low amniotic fluid levels during the summer, possibly because of dehydration. (scienceandsensibility.org)
  • Pluripotency can be rapidly and efficiently induced in human amniotic fluid-derived cells," Human Molecular Genetics , vol. 18, no. 22, pp. 4340-4349, 2009. (hindawi.com)
  • In this experimental study, we investigated the ability of human amniotic fluid derived cells to substitute dermal fibroblasts and support epidermis formation and stratification in a humanized animal model. (uzh.ch)
  • A woman who passes her due date by two or more weeks is at risk for low amniotic fluid. (gynob.com)
  • In addition, it is also known that the composition, the morphology, and the growth properties of amniotic-fluid cell samples are affected by certain fetal pathologies, such as for example, neural tube defects or gastroschisis [ 1 - 3 ]. (hindawi.com)
  • This test is intended for amniotic fluid samples that does not have any accompanying cytogenetic test order (e.g. chromosome analysis) but cell culture is desired for other molecular genetic or biochemical testing, such as familial mutation analysis, DNA isolation and storage, and send-out testing for molecular genetic/biochemical testing to be done on cultured amniocytes. (nationwidechildrens.org)
  • This was an in silico (computational) investigation using publicly available gene expression data previously produced by our group from 12 euploid midtrimester amniotic fluid samples. (lww.com)
  • There were 476 well-annotated genes present in 12 of 12 amniotic fluid samples. (lww.com)
  • The authors thank Keiko Koide, MD, a former member of our laboratory, who produced the original microarray data sets, and Janet Cowan, PhD, who provided the amniotic fluid supernatant samples and patient data. (lww.com)
  • Anthony Atala, M.D., Director of the Wake Forest Institute for Regenerative Medicine, has stated that 99% of the U.S. population could conceivably find genetic matches for tissue regeneration or engineered organs from just 100,000 unique amniotic fluid samples. (parentsguidecordblood.org)
  • The present invention refers to a fast method for the determination of phospholipids in amniotic fluid samples by HPTLC (high performance thin layer chromatography). (ideaconnection.com)
  • Amniotic fluid samples were provided by the Fetal Center at Texas Children's Hospital Pavilion for Women. (springer.com)
  • This protocol is designed for purification of DNA from 1-3 ml samples of amniotic fluid containing 0.5-2 x 10 6 cells using the Gentra Puregene Tissue Kit or Gentra Puregene Mouse Tail Kit. (qiagen.com)
  • We aimed to detect and sequence the Zika virus genome in amniotic fl uid samples of two pregnant women in Brazil whose fetuses were diagnosed with microcephaly. (org.in)
  • Viral reads were sparse in the amniotic fluid, and we found no evidence of a core viral community across samples. (biomedcentral.com)
  • Uncentrifuged fresh amniotic fluid samples were obtained during ARM/amniotomy and used for AFOD estimation at 650 nm. (ajol.info)
  • Currently, the main genotyping method is based on single copy microsatellite markers, which limit direct genotyping from amniotic fluids (AFs) to samples with a high parasitic load. (biomedcentral.com)
  • A subpopulation of the cells in the amniotic fluid, referred to as c-kit+, can be expanded to more than 250 population doublings while maintaining normal chromosones and long telomeres. (parentsguidecordblood.org)
  • Breathing problems can progress to acute respiratory failure a severe, life-threatening complication where damage and fluid leakage into the lungs makes breathing difficult or impossible. (rarediseases.org)
  • The amniotic fluid is essential for the development of muscles, limbs, lungs, and the digestive system. (americanpregnancy.org)
  • Fluid is produced by the fetal lungs and kidneys. (chop.edu)
  • Amniotic fluid is important in the development of fetal organs, especially the lungs. (chop.edu)
  • Too little fluid for long periods may cause abnormal or incomplete development of the lungs called pulmonary hypoplasia. (chop.edu)
  • The nutrients present in the fluid aid in the development of limbs, muscles, lungs and the digestive system of the baby. (momjunction.com)
  • Although human amniotic fluid cells are widely used in routine prenatal diagnosis, the knowledge about these cells remains limited. (hindawi.com)
  • Amniotic fluid has been used for more than 70 years for prenatal diagnosis (1). (parentsguidecordblood.org)
  • In fact, by means of prenatal diagnosis, a number of fetuses affected by chromosomal or Mendelian diseases can be identified, and the amniotic fluid collected for genetic testing can be used, after diagnosis, for the isolation, culture and differentiation of AFS cells. (mdpi.com)
  • Holm J, Ponders L, Sweetman L (1989) Prenatal diagnosis of propionic and methylmalonic acidaemia by stable isotope dilution analysis of amniotic fluid. (springer.com)
  • Jakobs C, Ten Brink HJ, Stellaard F (1990) Prenatal diagnosis of inherited metabolic disorders by quantitation of characteristic metabolites in amniotic fluid: facts and future. (springer.com)
  • Naylor G, Sweetman L, Nyhan WL et al (1980) Isotope dilution analysis of methylcitric acid in amniotic fluid for the prenatal diagnosis of propionic and methylmalonic acidemia. (springer.com)
  • Human amniotic fluid cells (HuAFCs) are currently used for the routine prenatal genetic diagnosis of a wide range of foetal abnormalities [ 9 , 10 ]. (medsci.org)
  • While in the womb, the baby floats in the amniotic fluid. (medlineplus.gov)
  • Amniotic fluid protects and nourishes the baby in the womb. (medicalnewstoday.com)
  • It is still unclear whether stem cells from amniotic fluid -- the liquid that cushions fetuses in the womb -- can produce the range of cell types that embryonic stem cells can. (latimes.com)
  • Amniotic fluid is the warm, fluid cushion that protects and supports your baby as they grow in the womb. (healthline.com)
  • Amniotic fluid surrounding unborn babies in the womb contains surfactant, a chemical critical for lung function in newborns. (chicagotribune.com)
  • As the baby grows he or she will move and tumble in the womb with the help of the amniotic fluid. (americanpregnancy.org)
  • Stem cells nearly as powerful as embryonic stem cells can be found in the amniotic fluid that protects babies in the womb, U.S. researchers reported. (freedomsphoenix.com)
  • Since the baby inside his mothers womb is suspended in the amniotic fluid, doesn't the amniotic fluid enter the nose, ears, eyes, mouth and other distinct pores of the baby ? (ndtv.com)
  • The constant temperature in the womb is also governed by the amniotic fluid. (gynob.com)
  • A tiny baby floating in a liquidy womb - and featuring prominently in this mesmerizing image is the amniotic fluid! (curejoy.com)
  • The uterus is divided into four quadrants, and the fluid is measured in each quadrant. (momjunction.com)
  • The measurements are then added to check the total amount of fluid in the uterus. (momjunction.com)
  • These can be caused by a defect in the shape of the uterus or the development of the amniotic sac, but a bacterial infection can also be the culprit for this condition. (md-health.com)
  • Amniotic fluid begins to form almost as soon as the embryo implants itself in the uterus wall. (curejoy.com)
  • Intrauterine growth restriction (poor fetal growth) is also associated with decreased amounts of amniotic fluid. (chop.edu)
  • However, the notion that undifferentiated and differentiated cells of varying origins and lineages are present in amniotic fluid has been supported by several reports over the last three decades. (hindawi.com)
  • Determination of fetal maturity by spectrophotometric analysis of amniotic fluid. (springer.com)
  • See detailed information below for a list of 54 causes of Excess amniotic fluid , Symptom Checker , including diseases and drug side effect causes. (rightdiagnosis.com)
  • Listed below are some combinations of symptoms associated with Excess amniotic fluid, as listed in our database. (rightdiagnosis.com)
  • Review further information on Excess amniotic fluid Treatments . (rightdiagnosis.com)
  • How Common are these Causes of Excess amniotic fluid? (rightdiagnosis.com)
  • This information refers to the general prevalence and incidence of these diseases, not to how likely they are to be the actual cause of Excess amniotic fluid. (rightdiagnosis.com)
  • The following list of conditions have ' Excess amniotic fluid ' or similar listed as a symptom in our database. (rightdiagnosis.com)
  • Many of these risk factors could be directly related to "the presumed causal roles of strong uterine contractions, excess amniotic fluid, and disruption of the uterine vasculature," the researchers noted. (mdedge.com)
  • Fujiwara T, Takahashi H (1989) Assessment of fetal lung maturity: stable microbubble test in amniotic fluid and gastric aspirate. (springer.com)