Amnion: The innermost membranous sac that surrounds and protects the developing embryo which is bathed in the AMNIOTIC FLUID. Amnion cells are secretory EPITHELIAL CELLS and contribute to the amniotic fluid.Extraembryonic Membranes: The thin layers of tissue that surround the developing embryo. There are four extra-embryonic membranes commonly found in VERTEBRATES, such as REPTILES; BIRDS; and MAMMALS. They are the YOLK SAC, the ALLANTOIS, the AMNION, and the CHORION. These membranes provide protection and means to transport nutrients and wastes.Chorion: The outermost extra-embryonic membrane surrounding the developing embryo. In REPTILES and BIRDS, it adheres to the shell and allows exchange of gases between the egg and its environment. In MAMMALS, the chorion evolves into the fetal contribution of the PLACENTA.Labor, Obstetric: The repetitive uterine contraction during childbirth which is associated with the progressive dilation of the uterine cervix (CERVIX UTERI). Successful labor results in the expulsion of the FETUS and PLACENTA. Obstetric labor can be spontaneous or induced (LABOR, INDUCED).Amniotic Fluid: A clear, yellowish liquid that envelopes the FETUS inside the sac of AMNION. In the first trimester, it is likely a transudate of maternal or fetal plasma. In the second trimester, amniotic fluid derives primarily from fetal lung and kidney. Cells or substances in this fluid can be removed for prenatal diagnostic tests (AMNIOCENTESIS).Parturition: The process of giving birth to one or more offspring.Pregnancy: The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.Amniotic Band Syndrome: A disorder present in the newborn infant in which constriction rings or bands, causing soft tissue depressions, encircle digits, extremities, or limbs and sometimes the neck, thorax, or abdomen. They may be associated with intrauterine amputations.Decidua: The hormone-responsive glandular layer of ENDOMETRIUM that sloughs off at each menstrual flow (decidua menstrualis) or at the termination of pregnancy. During pregnancy, the thickest part of the decidua forms the maternal portion of the PLACENTA, thus named decidua placentalis. The thin portion of the decidua covering the rest of the embryo is the decidua capsularis.Placenta: A highly vascularized mammalian fetal-maternal organ and major site of transport of oxygen, nutrients, and fetal waste products. It includes a fetal portion (CHORIONIC VILLI) derived from TROPHOBLASTS and a maternal portion (DECIDUA) derived from the uterine ENDOMETRIUM. The placenta produces an array of steroid, protein and peptide hormones (PLACENTAL HORMONES).Labor Onset: The beginning of true OBSTETRIC LABOR which is characterized by the cyclic uterine contractions of increasing frequency, duration, and strength causing CERVICAL DILATATION to begin (LABOR STAGE, FIRST ).Fetal Membranes, Premature Rupture: Spontaneous tearing of the membranes surrounding the FETUS any time before the onset of OBSTETRIC LABOR. Preterm PROM is membrane rupture before 37 weeks of GESTATION.Serous Membrane: A thin lining of closed cavities of the body, consisting of a single layer of squamous epithelial cells (MESOTHELIUM) resting on a thin layer of CONNECTIVE TISSUE, and covered with secreted clear fluid from blood and lymph vessels. Major serous membranes in the body include PERICARDIUM; PERITONEUM; and PLEURA.Cellulose, Oxidized: A cellulose of varied carboxyl content retaining the fibrous structure. It is commonly used as a local hemostatic and as a matrix for normal blood coagulation.Reptiles: Cold-blooded, air-breathing VERTEBRATES belonging to the class Reptilia, usually covered with external scales or bony plates.Chorionic Villi Sampling: A method for diagnosis of fetal diseases by sampling the cells of the placental chorionic villi for DNA analysis, presence of bacteria, concentration of metabolites, etc. The advantage over amniocentesis is that the procedure can be carried out in the first trimester.Limb Deformities, Congenital: Congenital structural deformities of the upper and lower extremities collectively or unspecified.Birds: Warm-blooded VERTEBRATES possessing FEATHERS and belonging to the class Aves.Natural Childbirth: Labor and delivery without medical intervention, usually involving RELAXATION THERAPY.Genetics, Medical: A subdiscipline of human genetics which entails the reliable prediction of certain human disorders as a function of the lineage and/or genetic makeup of an individual or of any two parents or potential parents.Genetic Counseling: An educational process that provides information and advice to individuals or families about a genetic condition that may affect them. The purpose is to help individuals make informed decisions about marriage, reproduction, and other health management issues based on information about the genetic disease, the available diagnostic tests, and management programs. Psychosocial support is usually offered.Genetic Diseases, Inborn: Diseases that are caused by genetic mutations present during embryo or fetal development, although they may be observed later in life. The mutations may be inherited from a parent's genome or they may be acquired in utero.Genetic Testing: Detection of a MUTATION; GENOTYPE; KARYOTYPE; or specific ALLELES associated with genetic traits, heritable diseases, or predisposition to a disease, or that may lead to the disease in descendants. It includes prenatal genetic testing.Rhytidoplasty: Plastic surgery performed, usually by excision of skin, for the elimination of wrinkles from the skin.Chiroptera: Order of mammals whose members are adapted for flight. It includes bats, flying foxes, and fruit bats.Rabies: Acute VIRAL CNS INFECTION affecting mammals, including humans. It is caused by RABIES VIRUS and usually spread by contamination with virus-laden saliva of bites inflicted by rabid animals. Important animal vectors include the dog, cat, bat, fox, raccoon, skunk, and wolf.Rabies virus: The type species of LYSSAVIRUS causing rabies in humans and other animals. Transmission is mostly by animal bites through saliva. The virus is neurotropic multiplying in neurons and myotubes of vertebrates.Bites and StingsMicrobial Collagenase: A metalloproteinase which degrades helical regions of native collagen to small fragments. Preferred cleavage is -Gly in the sequence -Pro-Xaa-Gly-Pro-. Six forms (or 2 classes) have been isolated from Clostridium histolyticum that are immunologically cross-reactive but possess different sequences and different specificities. Other variants have been isolated from Bacillus cereus, Empedobacter collagenolyticum, Pseudomonas marinoglutinosa, and species of Vibrio and Streptomyces. EC 3.4.24.3.Tissue Inhibitor of Metalloproteinases: A family of secreted protease inhibitory proteins that regulates the activity of SECRETED MATRIX METALLOENDOPEPTIDASES. They play an important role in modulating the proteolysis of EXTRACELLULAR MATRIX, most notably during tissue remodeling and inflammatory processes.Fibroblasts: Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.Skin: The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.Collagenases: Enzymes that catalyze the degradation of collagen by acting on the peptide bonds.Biological Dressings: Human or animal tissue used as temporary wound coverings.International Classification of Diseases: A system of categories to which morbid entries are assigned according to established criteria. Included is the entire range of conditions in a manageable number of categories, grouped to facilitate mortality reporting. It is produced by the World Health Organization (From ICD-10, p1). The Clinical Modifications, produced by the UNITED STATES DEPT. OF HEALTH AND HUMAN SERVICES, are larger extensions used for morbidity and general epidemiological purposes, primarily in the U.S.Databases, Factual: Extensive collections, reputedly complete, of facts and data garnered from material of a specialized subject area and made available for analysis and application. The collection can be automated by various contemporary methods for retrieval. The concept should be differentiated from DATABASES, BIBLIOGRAPHIC which is restricted to collections of bibliographic references.Clinical Coding: Process of substituting a symbol or code for a term such as a diagnosis or procedure. (from Slee's Health Care Terms, 3d ed.)Fetus: The unborn young of a viviparous mammal, in the postembryonic period, after the major structures have been outlined. In humans, the unborn young from the end of the eighth week after CONCEPTION until BIRTH, as distinguished from the earlier EMBRYO, MAMMALIAN.Insurance Claim Review: Review of claims by insurance companies to determine liability and amount of payment for various services. The review may also include determination of eligibility of the claimant or beneficiary or of the provider of the benefit; determination that the benefit is covered or not payable under another policy; or determination that the service was necessary and of reasonable cost and quality.Fetal Blood: Blood of the fetus. Exchange of nutrients and waste between the fetal and maternal blood occurs via the PLACENTA. The cord blood is blood contained in the umbilical vessels (UMBILICAL CORD) at the time of delivery.Mesenchymal Stromal Cells: Bone-marrow-derived, non-hematopoietic cells that support HEMATOPOETIC STEM CELLS. They have also been isolated from other organs and tissues such as UMBILICAL CORD BLOOD, umbilical vein subendothelium, and WHARTON JELLY. These cells are considered to be a source of multipotent stem cells because they include subpopulations of mesenchymal stem cells.Hematopoietic Stem Cells: Progenitor cells from which all blood cells derive.Antigens, CD34: Glycoproteins found on immature hematopoietic cells and endothelial cells. They are the only molecules to date whose expression within the blood system is restricted to a small number of progenitor cells in the bone marrow.Cord Blood Stem Cell Transplantation: Transplantation of STEM CELLS collected from the fetal blood remaining in the UMBILICAL CORD and the PLACENTA after delivery. Included are the HEMATOPOIETIC STEM CELLS.Mesenchymal Stem Cell Transplantation: Transfer of MESENCHYMAL STEM CELLS between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS).Receptors, Oxytocin: Cell surface proteins that bind oxytocin with high affinity and trigger intracellular changes which influence the behavior of cells. Oxytocin receptors in the uterus and the mammary glands mediate the hormone's stimulation of contraction and milk ejection. The presence of oxytocin and oxytocin receptors in neurons of the brain probably reflects an additional role as a neurotransmitter.Oxytocin: A nonapeptide hormone released from the neurohypophysis (PITUITARY GLAND, POSTERIOR). It differs from VASOPRESSIN by two amino acids at residues 3 and 8. Oxytocin acts on SMOOTH MUSCLE CELLS, such as causing UTERINE CONTRACTIONS and MILK EJECTION.Vasotocin: A nonapeptide that contains the ring of OXYTOCIN and the side chain of ARG-VASOPRESSIN with the latter determining the specific recognition of hormone receptors. Vasotocin is the non-mammalian vasopressin-like hormone or antidiuretic hormone regulating water and salt metabolism.LondonTocolytic Agents: Drugs that prevent preterm labor and immature birth by suppressing uterine contractions (TOCOLYSIS). Agents used to delay premature uterine activity include magnesium sulfate, beta-mimetics, oxytocin antagonists, calcium channel inhibitors, and adrenergic beta-receptor agonists. The use of intravenous alcohol as a tocolytic is now obsolete.SwitzerlandDevelopmental Biology: The field of biology which deals with the process of the growth and differentiation of an organism.

Prostaglandin endoperoxide H synthase mRNA expression in the human amnion and decidua during pregnancy and in the amnion at preterm labour. (1/868)

We have examined the expression of prostaglandin endoperoxide H synthase (PGHS) isoenzymes in the amnion and the decidua during gestation, and the abundance of PGHS mRNA in the amnion at idiopathic preterm labour. PGHS-1 and -2 mRNA abundance in the amnion, determined with ribonuclease protection assays, was significantly (P< 0.05) higher at term than earlier during pregnancy. In contrast, neither PGHS-1 and -2 mRNA values, nor PGHS-specific activity, measured with a cell-free assay, was different in the decidua at term as compared to earlier gestational ages. In individual term patients, PGHS-2 mRNA values in the amnion were positively correlated with PGHS-2 mRNA values in the chorion laeve. PGHS-1 and -2 mRNA abundance was higher (P < 0.05) in the amnion after idiopathic preterm labour than in the absence of labour at the same gestational age (28-35 weeks). Thus, PGHS-1 and -2 are induced in the amnion at term. Furthermore, amniotic PGHS-2 changes in co-ordination with PGHS-2 concentrations in the chorion laeve. PGHS is not induced in the decidua at term. Increased amniotic PGHS expression may contribute to the enhanced intrauterine prostaglandin synthesis before term labour. Both PGHS isoenzymes may participate in the increase of PGHS activity in the amnion at preterm birth.  (+info)

First-trimester cord entanglement in monoamniotic twins. (2/868)

OBJECTIVE: Monoamniotic twinning occurs in only 1% of twin pregnancies, but carries a high perinatal mortality rate. Early and reliable diagnosis is essential if attempts are to be made to reduce the complication rate. We report color Doppler demonstration of cord entanglement in the first trimester, which is diagnostic of monoamnionicity. METHODS: Two patients with twin pregnancies were examined in the first trimester with pulsed and color Doppler insonation of their umbilical arteries. RESULTS: Cord entanglement was suspected and proved by demonstrating differing fetal heart rate patterns in the same direction on umbilical artery Doppler analysis of a common mass of cord vessels. Following appropriate counselling, medical amnioreduction was induced at 20 weeks of gestation to reduce fetal movements and worsening cord entanglement. Delivery was by elective Cesarean section at 32 weeks' gestation and monoamnionicity was confirmed. CONCLUSION: We report a new sign for the demonstration of monoamnionicity in twin pregnancies in the first trimester. This should improve the reliability of early diagnosis, but further studies are required to confirm that, if cord entanglement occurs, it is usually present by the end of the first trimester.  (+info)

Tissue plasminogen activator and its receptor in the human amnion, chorion, and decidua at preterm and term. (3/868)

The plasminogen activator system consists of two proteins: tissue plasminogen activator (tPA) and urokinase plasminogen activator (uPA), which act upon their specific receptors to generate plasmin from plasminogen located on the cell surface. Plasmin then acts directly and indirectly to degrade the components of the extracellular matrix (ECM). This process is likely to be important in the normal turnover of the ECM of fetal membranes and in its premature weakening in preterm premature rupture of the fetal membranes. Quantitative Northern analysis and in situ hybridization have shown that the decidua expresses mRNA for tPA. However, the immunolocalized tPA protein was most strongly associated with the amnion and chorion, as was its receptor annexin II, suggesting that the amnion and chorion are the targets for decidual tPA. At term, decidual tPA expression was unaffected by labor, and the tPA receptor was elevated both before and after labor. At preterm, the converse was found: decidual tPA expression was significantly (p < 0. 05) up-regulated by labor, but the tPA receptor was not. The results suggest that the generation of plasmin at term would be controlled by an increased concentration of the tPA receptor in the amnion and chorion, whereas at preterm a pathological increase in plasmin would be generated by an overexpression of tPA, initiated by labor.  (+info)

Conjunctival epithelial cell differentiation on amniotic membrane. (4/868)

PURPOSE: Amniotic membrane (AM)-reconstructed conjunctival surfaces recover the normal epithelial phenotype with a significantly higher cell density than the control. The present study was undertaken to examine how AM modulates rabbit conjunctival epithelial cell differentiation. METHODS: Rabbit conjunctival epithelial cells (RCEs) were cultured on the basement membrane side of dispase-pretreated AM, with or without seeding rabbit conjunctival fibroblasts (RCFs) on the stromal side. After 7 to 12 days, half of the cultures were raised to the air-liquid interface, and the remainder stayed submerged. A small group of air-lifted cultures containing RCFs was treated with retinoic acid. After 1, 2, and 4 weeks, cultures were terminated and processed for immunostaining with antibodies directed against distinct types of mucins (SMC and AM3), glycocalyx (AMEM2), keratin K3 (AE5), and K12 (AK2). Additionally, western blot analysis was performed for K3 keratin expression. Ultrastructural changes were evaluated by transmission electron microscopy. RESULTS: In general, RCEs grown on AM were uniformly small, negative to AE5 and AK2 antibodies, and positive to AMEM2 and ASPG1 antibodies. Epithelial stratification and cell polarity with prominent microvilli, tight junctions, and hemidesmosomes were more pronounced in air-lifted cultures. RCEs cocultured with RCFs showed scattered AM3-positive goblet cells, which were not increased by retinoic acid. CONCLUSIONS: RCEs cultured on AM primarily exhibit a nongoblet conjunctival epithelial phenotype. Epithelial stratification and cell polarity, features essential for epithelial differentiation, are promoted by air-lifting. This culture model will be useful for studying how growth and differentiation of conjunctival epithelial cells can be modulated further.  (+info)

Expression of cyclo-oxygenase types-1 and -2 in human fetal membranes throughout pregnancy. (5/868)

Human labour is associated with increased prostaglandin synthesis within the fetal membranes. We have studied the expression of the two isoforms of the central prostaglandin synthetic enzyme, cyclo-oxygenase (COX-1 and COX-2), in human fetal membranes throughout pregnancy, at mRNA, protein and activity levels. COX-1 mRNA expression was low in human amnion and chorion-decidua and did not change with gestational age. COX-2 mRNA expression in fetal membranes increased with gestational age, with significant up-regulation prior to the onset of labour and in association with labour. Protein concentrations of COX-1 did not change, whilst concentrations of COX-2 increased from the first to the third trimester. COX activity increased with gestational age and in association with labour, although prostaglandin production in fetal membranes collected after labour was reduced, suggesting reduced substrate supply. These data suggest that it is up-regulation of COX-2, rather than of COX-1, which mediates increased prostaglandin synthesis within the fetal membranes at term. Much of the increase in COX-2 expression precedes the onset of labour, suggesting that it is a cause, rather than a consequence, of labour.  (+info)

Amnion-derived cells express intercellular adhesion molecule-1: regulation by cytokines. (6/868)

We have examined the expression of the intercellular adhesion molecule-1 (ICAM-1) mRNA in primary and established amnion-derived cell cultures and regulation of this expression by tumour necrosis factor-alpha (TNF-alpha) and interleukin (IL)-1beta. TNF-alpha (50 ng/ml) and IL-1beta (1.0 ng/ml) induced 18- and 11-fold increases respectively in expression of the ICAM-1 mRNA in WISH cells (an amnion epithelium-derived cell line). The increase was detectable within one hour of treatment and peaked by two hours. The protein synthesis inhibitor, cycloheximide (10 microg/ml) did not inhibit this induction. Increased levels of ICAM-1 protein were detected in the cells within 4 h after initiation of treatment with either cytokine. By 16 h of treatment with IL-1beta or TNF-alpha ICAM-1 reached 40 and 73 pg/microg cellular protein, representing 6- and 11-fold stimulations respectively. In primary amnion cells, basal expression of ICAM-1 mRNA was undetectable. However, TNF-alpha (50 ng/ml) induced ICAM-1 mRNA within two hours, peak expression being reached between four and eight hours after initiation of treatment. The present report demonstrates for the first time that amnion derived cells can express ICAM-1 and, further, that this expression is regulated by pro-inflammatory cytokines. This has implications for the amnion as a possible source for soluble ICAM-1, for this gene product as a marker for preterm labour, and for participation of the amnion, additional to its reported secretory role, in inflammatory processes of the fetal membranes.  (+info)

Oxytocin receptor expression in human term and preterm gestational tissues prior to and following the onset of labour. (7/868)

Oxytocin receptor (OTR) mRNA expression has previously been demonstrated in human myometrium, decidua, chorion and amnion but the effect of gestational age and the onset of labour has not been determined in these individual tissues. Spatial OTR mRNA expression was examined by in situ hybridization and ligand binding was confirmed using autoradiography with the iodinated oxytocin antagonist d(CH2)5[Tyr(Me)2,Thr4,Tyr-NH29]-vasotocin (125I-OTA). Tissue was collected at term (>37 weeks of gestation) or preterm (24-36 weeks of gestation) caesarean section and classified as labour (contractions every 5 min associated with cervical dilatation) or non-labour. OTR mRNA expression was measured as optical density units from autoradiographs. There was a highly significant (P<0.001) effect of tissue type on expression of OTR mRNA with expression greatest in myometrium, low in decidua and chorion and not detected in placenta. Similar results were obtained with the 125I-OTA-binding studies, indicating that the message was translated. Amnion had an apparently high level of both hybridization and 125I-OTA binding in some samples, but a lack of specificity prevented quantification of the signal in this tissue type. Term myometrium (labour and non-labour) had significantly higher (P<0.01) OTR mRNA expression than preterm myometrium, but there was no further increase in mRNA concentration associated with labour onset. In contrast, 125I-OTA binding in myometrium was already high at 33 weeks and did not increase further either later in pregnancy or with labour. In decidua there was no effect of gestational age or labour onset on OTR mRNA expression or 125I-OTA binding. In summary, OTR mRNA expression in the myometrium increased in late pregnancy whereas decidual expression was much lower and did not rise at term.  (+info)

Human RNA-specific adenosine deaminase ADAR1 transcripts possess alternative exon 1 structures that initiate from different promoters, one constitutively active and the other interferon inducible. (8/868)

RNA-specific adenosine deaminase (ADAR1) catalyzes the deamination of adenosine to inosine in viral and cellular RNAs. Two size forms of the ADAR1 editing enzyme are known, an IFN-inducible approximately 150-kDa protein and a constitutively expressed N-terminally truncated approximately 110-kDa protein. We have now identified alternative exon 1 structures of human ADAR1 transcripts that initiate from unique promoters, one constitutively expressed and the other IFN inducible. Cloning and sequence analyses of 5'-rapid amplification of cDNA ends (RACE) cDNAs from human placenta established a linkage between exon 2 of ADAR1 and two alternative exon 1 structures, designated herein as exon 1A and exon 1B. Analysis of RNA isolated from untreated and IFN-treated human amnion cells demonstrated that exon 1B-exon 2 transcripts were synthesized in the absence of IFN and were not significantly altered in amount by IFN treatment. By contrast, exon 1A-exon 2 transcripts were IFN inducible. Transient transfection analysis with reporter constructs led to the identification of two functional promoters, designated PC and PI. Exon 1B transcripts were initiated from the PC promoter whose activity in transient transfection reporter assays was not increased by IFN treatment. The 107-nt exon 1B mapped 14.5 kb upstream of exon 2. The 201-nt exon 1A that mapped 5.4 kb upstream of exon 2 was initiated from the interferon-inducible PI promoter. These results suggest that two promoters, one IFN inducible and the other not, initiate transcription of the ADAR1 gene, and that alternative splicing of unique exon 1 structures to a common exon 2 junction generates RNA transcripts with the deduced coding capacity for either the constitutively expressed approximately 110-kDa ADAR1 protein (exon 1B) or the interferon-induced approximately 150-kDa ADAR1 protein (exon 1A).  (+info)

TY - JOUR. T1 - Nrf2 activation inhibits effects of thrombin in human amnion cells and thrombin-induced preterm birth in mice. AU - Chigusa, Yoshitsugu. AU - Kishore, Annavarapu Hari. AU - Mogami, Haruta. AU - Word, Ruth Ann. PY - 2016/6/1. Y1 - 2016/6/1. N2 - Context: Nrf2 is a key transcription factor that modulates cell defense mechanisms against endogenous and exogenous stress. Previously, we reported that thrombin increased matrix metalloproteinases and prostaglandin synthesis in human amnion mesenchymal cells. Objective: We sought to determine whether activation of Nrf2 alters the effect of thrombin on prostaglandin synthesis, protease activation, and cytokine release in human amnion. Furthermore, we analyzed the effect of Nrf2 activation on thrombin-induced preterm labor in mice. Design: Primary human amnion mesenchymal cells and pregnant mice were employed to investigate the effect of Nrf2 on thrombin-induced inflammation and preterm birth. Setting: This was a laboratory-based study ...
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Purpose: : To evaluate the efficacy of early sutureless amniotic membrane transplantation in the management of severe bacterial keratitis to reduce pain, inflammation, and haze, and to promote healing. Methods: : A non-comparative case series including 3 eyes of 3 consecutive patients with severe bacterial keratitis exhibiting persistent epithelial defect/ulcer were retrospectively reviewed following early sutureless amniotic membrane transplantation via ProKeraTM together with selective topical antibiotics and preservative-free steroid. Pain relief, inflammation, haze, and corneal epithelial healing were monitored. Results: : ProKeraTM was inserted once in one eye, twice in the other two eyes. Pain was significantly relieved, and inflammation was markedly reduced in all cases. The corneal epithelial defect and stromal ulceration rapidly healed while visual acuity improved in two out of the 3 eyes. Conclusions: : Temporary sutureless amniotic membrane transplantation via ProKeraTM allows easy ...
TY - JOUR. T1 - Evidence for active acetylcholine metabolism in human amniotic epithelial cells. T2 - Applicable to intracerebral allografting for neurologic disease. AU - Sakuragawa, Norio. AU - Misawa, Hidemi. AU - Ohsugi, Keiko. AU - Kakishita, Kouji. AU - Ishii, Takashi. AU - Thangavel, Ramasamy. AU - Tohyama, Jun. AU - Elwan, Mohamed. AU - Yokoyama, Yasunobu. AU - Okuda, Osamu. AU - Arai, Hajime. AU - Ogino, Ikuko. AU - Sato, Kiyoshi. PY - 1997/8/22. Y1 - 1997/8/22. N2 - Human amniotic epithelial (HAE) cells have been used for allotransplantation in patients with lysosomal storage disease due to lack of expression of HLA antigens. Previously, we have reported the expression of differentiation markers for both neural stem cells, and neuron and glial cells. In the present study, we investigated the presence of choline acetyltransferase (CHAT) and acetylcholine (ACh) in HAE cells using different experimental approaches. Cultured HAE cells showed strong immunoreactivity against ChAT antibody. ...
TY - JOUR. T1 - Inherent propensity of amnion-derived mesenchymal stem cells towards endothelial lineage. T2 - Vascularization from an avascular tissue. AU - Warrier, S.. AU - Haridas, N.. AU - Bhonde, R.. PY - 2012/10. Y1 - 2012/10. N2 - One of the most pressing problems in injury is wound healing and blood vessel formation. The amniotic membrane is important in clinical applications as it is pro-angiogenic, anti-fibrotic and anti-scarring and has low immunogenicity. In this study, we characterized amniotic membrane mesenchymal stem cells (AMMSCs) by their trademark mesenchymal stem cell (MSC) signature and profiled for embryonic pluripotency markers namely alkaline phosphatase, Oct4, Sox2, Nanog, SSEA3 and 4, and Klf4 by RT-PCR and nuclear localization of Oct4 and Nanog by immunocytochemistry. The amnion, although avascular, contains pro-angiogenic factors such as type I, III, IV and V collagen, laminin, and fibronectin in the extra cellular matrix. We, therefore, hypothesized that AMMSCs is ...
Purpose: : To examine the characteristics of iris pigment epithelial (IPE) cells cultured on amniotic membrane (AM) and to determine whether subretinal transplantation of this IPE cell sheet can protect photoreceptor cell loss in dystrophic Royal College of Surgeons (RCS) rats. Methods: : Human and rat IPE cells were cultured on the basement membrane side of dispase treated AM. After 2 weeks of seeding, the expression of several genes participate in the function of differentiated retinal pigment epithelial (RPE) cells was evaluated using real time PCR and Western blotting. Ultrastructural changes were evaluated using transmission electron microscopy. The IPE cell sheet cultured on AM was transplanted into the subretinal space of 4-week-old RCS rats, and the eyes were analyzed histologically at 12 weeks after grafting. The effect of transplantation of IPE cell sheet on the visual function was estimated by optokinetic reflex. Results: : IPE cells cultured on AM exhibited ultrastructural epithelial ...
TY - JOUR. T1 - The effects of thrombin and cytokines upon the biomechanics and remodeling of isolated amnion membrane, in vitro. AU - Kumar, D.. AU - Schatz, F.. AU - Moore, R. M.. AU - Mercer, B. M.. AU - Rangaswamy, N.. AU - Mansour, J. M.. AU - Lockwood, C. J.. AU - Moore, J. J.. PY - 2011/3. Y1 - 2011/3. N2 - Abruption-induced thrombin generation and inflammation/infection induced cytokine production have both been associated with fetal membrane (FM) weakening and preterm premature rupture of the fetal membranes (PPROM). Using our in vitro model system we have demonstrated that thrombin, and separately the cytokines, tumor necrosis factor-alpha (TNFα) and interleukin-1-beta (IL-1β), remodel and weaken full thickness FM. Additionally, we have reported that the anti-oxidant and NFκB inhibitor, alpha-lipoic acid (LA), blocks these thrombin and cytokine induced effects. The purpose of these studies was to determine whether thrombin and cytokines directly weaken the amnion membrane (AM), the ...
TY - JOUR. T1 - Denuded human amniotic membrane seeding bone marrow stromal cells as an effective composite matrix stimulates axonal outgrowth of rat neural cortical cells in vitro. AU - Liang, Hong Sheng. AU - Liang, Peng. AU - Xu, Ye. AU - Wu, Jia Ning. AU - Liang, Tao. AU - Xu, Xiao Ping. AU - Liu, En Zhong. PY - 2009/9. Y1 - 2009/9. N2 - Background: Previous studies have shown that axonal outgrowth in the damaged central nervous system is closely related to the local microenvironment. Transplantation of bone marrow stromal cells (BMSC) or BMSC with some biomaterials has been used to treat various central nervous system diseases with some success. In the current study, we investigated if BMSC on denuded human amniotic membrane (DhAM) as a composite matrix could stimulate axonal outgrowth or not. Method: After completely removing the cells on the amniotic membrane with a tryptic and mechanical approach, we seeded BMSC on it. The MTS was applied to test the cytotoxicity of DhAM compared with ...
Amniotic membrane graft to conjunctival flap in treatment of non-viral resistant infectious keratitis: a randomised clinical study ...
Objective: To evaluate the use of hypothermically stored human amniotic membrane for cartilage repair in adult sheep. Studies show that human amniotic membrane contains pluripotent mesenchymal stem cells that can be influenced to produce chondrocytes. It is unknown if human amniotic cells can produce hyaline-like cartilage. This study evaluates the use of hypothermically stored amniotic membrane (HSAM) to fill chondral defects in a sheep model. We hypothesized HSAM would fill defects with hyaline-like cartilage with chondrocytes populating the matrix. One sheep was used as a control, and four sheep received amniotic membrane. Two of these sheep were used as a normal control comparison. A 1 cm2 defect was created on the trochlear grove in all specimens. Each membrane was sized and laid over with the stromal layer facing the subchondral bone and covered with Fibrin sealant. The knees were harvested at five months and underwent morphological, histological, and immunohistological evaluation based on the
TY - JOUR. T1 - Paradox of glucocorticoid-induced cytosolic phospholipase A2 group IVA messenger RNA expression involves glucocorticoid receptor binding to the promoter in human amnion fibroblasts. AU - Guo, Chunming. AU - Yang, Zhen. AU - Li, Wenjiao. AU - Zhu, Ping. AU - Myatt, Leslie. AU - Sun, Kang. PY - 2008/1. Y1 - 2008/1. N2 - Glucocorticoids (GCs) are well-known anti-inflammatory drugs inhibiting prostaglandin production. Paradoxically, GCs are reported to stimulate cytosolic phosphoplipase A2 group IVA (PLA2G4A) and prostaglandin-endoperoxide synthase 2 (PTGS2) expression in human amnion fibroblasts. This study was designed to examine the molecular mechanisms underlying glucocorticoid-induced PLA2G4A expression in human amnion fibroblasts. Our data showed that cortisol (0.01∼1 μM) increased PLA2G4A mRNA level in a dose-dependent manner in human amnion fibroblasts, which was blocked by glucocorticoid receptor antagonist RU486 (1 μM) as well as by the mRNA transcription inhibitor ...
A reconstituted collagen membrane from human amnion has been investigated as a source of collagen matrix, which could be used as a substratum for culturing human fibroblasts. The suitability of pepsin-solubilized reconstituted human amniotic membrane, before and after cross-linking with chitosan, as a dermal matrix for culturing fibroblast was assessed by morphologic, physicochemical, cytotoxic and histochemical methods. Measurement of thermodynamic behaviour, by differential scanning calorimetric (DSC) and thermogravimetric analysis (TGA), and tensile strength suggested that the cross-linked membrane had sufficient elasticity to serve as an efficient dermal substrate for in vitro culture of fibroblasts. Fibroblasts cultured on the chitosan cross-linked collagen membrane had good adherence, retaining their morphology as indicated by microscopic analysis. Proliferation of fibroblasts, observed on this membrane affirms its non-toxic nature. These results support the application of reconstituted ...
Increasing evidence has shown that mesenchymal stem cells (MSCs) yield a favorable therapeutic benefit for thermal burn skin wounds. Human amniotic MSCs (hAMSCs) derived from amniotic membrane have multilineage differentiation, immunosuppressive, and anti-inflammatory potential which makes them suitable for treating skin wounds. However, the exact effects of hAMSCs on the healing of thermal burn skin wounds and their potential mechanisms are not explored. hAMSCs were isolated from amniotic membrane and characterized by RT-PCR, flow cytometry, immunofluorescence, and tumorigenicity test. We assessed the effects of hAMSCs and hAMSC conditional medium (CM) on wound healing in a deep second-degree burn injury model of mice. We then investigated the biological effects of hAMSCs and hAMSC-CM on the apoptosis and proliferation of heat stress-injured human keratinocytes HaCAT and dermal fibroblasts (DFL) both in vivo and in vitro. Next, we explored the underlying mechanisms by assessing PI3K/AKT and GSK3β/β
References: 1. Niknejad H, Peirovi H, Jorjani M, et al. Properties of the Amniotic Membrane For Potential Use in Tissue Engineering. European Cells and Materials. 2008(15):88-89. 2. Gruss J, Jirsch D. Human amniotic membrane: a versatile wound dressing. CMA Journal.1978(18);1237-1254. 3. Data on File, Nutech Medical. 4. Diaz-Prado A, Rendal-Vasques E, Muinos-Lopez E, et al. Potential use of the human amniotic membrane as a scaffold in articular cartilage repair. Cell Tissue Bank. 2010;11:183-195. 5. Garcia D, Longo U, Vaquero J, Forriol F, Loppini M, Khan W, Denaro V. Amniotic Membrane Transplant for Articular Cartilage Repair: An Experimental Study in Sheep. Current Stem Cell Research & Therapy. 2014;9(6);1-7. 6. Cooke, M, Tan, E, Mandrycky, C, He, H, OConnell, J, Tseng, S, Comparison Of Cryopreserved Amniotic Membrane And Umbilical Cord Tissue With Dehydrated Amniotic Membrane/Chorion Tissue, Journal Of Wound Care 2014, 23(10): 465-76.. Document No.: Affinity-1101 Revision: Initial Release ...
Synonyms for amnions in Free Thesaurus. Antonyms for amnions. 2 synonyms for amnion: amnios, amniotic sac. What are synonyms for amnions?
Objective: The aim of this research was to present the results of patients with the indication for treatment of cornea by amniotic membrane transplant over a four-year period, from 2012 to 2015. Study design: Retrospective cohort study. Participants and methods: The study included patients with pathological changes in the cornea, who have undergone amniotic membrane transplantation over the four-year period from 2012 to 2015, at the Department of Ophthalmology, University Hospital Center Osijek. The data for each patient were taken on the following: the indication for the amniotic membrane transplant, clinical findings on the cornea and visual acuity before and after the procedure, the possible reoperation, as well as the demographic characteristics. Results: Out of the total of 11 patients, 9 were women. Inflammation was the most common cause of clinical findings, in 5 patients. Infection was not found in 8 patients. Two patients complained about postoperative pain. Subjective improvement month ...
Wound healing is a complex pathophysiological process that involves a variety of cells and cytokines. In this study, we found that local injection of human amnion mesenchymal stem cells into wounds in rats could promote wound healing. Therefore, we hypothesized that the exosomes of human amnion mesenchymal stem cells contain substances that regulate the migration of epidermal cells. It has been reported that miR-135a is involved in cell migration and transformation. However, there have been no reports of its function in skin wound healing. To test this hypothesis, we injected exosomes overexpressing miR-135a directly into the wound margin. In addition, we tested the migration of BJ cells with overexpression or knockdown of miR-135a in vitro. Additionally, Western blot analysis was used to detect the expression of fibroblast migration-associated proteins after treatment with miR-135a overexpression or knockdown. MiR-135a significantly promoted wound healing compared to the control treatment. Western blot
Results Culture-expanded amnion-derived adherent cells expressed mesenchymal stem cell markers and HLA-ABC molecules and could differentiate into osteoblasts, adipocytes and chondrocyte-like cells, indicating that the cells have the characteristics of mesenchymal stem cells. The Lin-CD34+ cells purified from the frozen umbilical cord blood were strongly positive for HLA-ABC, and contained a large number of hematopoietic stem cells. When the Lin-CD34+ cells were cultured on the autologous (MHC-matched) or MHC-mismatched amnion-derived adherent cells in short-term assays (hematopoietic stem cell-proliferation) and long-term culture-initiating cell assays, greater expansion of the Lin-CD34+ cells was observed in the MHC-matched combination than in MHC-mismatched combinations. The concentration of granulocyte-macrophage colony-stimulating factor in the culture supernatants of the long-term culture-initiating cell assays was significantly higher in the MHC-matched combination than in MHC-mismatched ...
619PRTHuman Immunodeficiency Virus 1Arg Lys Lys Arg Arg Gln Arg Arg Arg1 529PRTHuman Immunodeficiency Virus 2Lys Lys Lys Lys Lys Lys Lys Lys Lys1 539PRTHuman Immunodeficiency Virus 3Arg Arg Arg Arg Arg Arg Arg Arg Arg1 54281PRTHomo sapiens 4Met Asn Gly Glu Glu Gln Tyr Tyr Ala Ala Thr Gln Leu Tyr Lys Asp1 5 10 15Pro Cys Ala Phe Gln Arg Gly Pro Ala Pro Glu Phe Ser Ala Ser Pro 20 25 30Pro Ala Cys Leu Tyr Met Gly Arg Gln Pro Pro Pro Pro Pro Pro His 35 40 45Pro Phe Pro Gly Ala Leu Gly Ala Glu Gln Gly Ser Pro Pro Asp Ile 50 55 60Ser Pro Tyr Glu Val Pro Pro Leu Ala Asp Asp Pro Ala Val Ala His65 70 75 80Leu His His His Leu Pro Ala Gln Leu Ala Leu Pro His Pro Pro Ala 85 90 95Gly Pro Phe Pro Glu Gly Ala Glu Pro Gly Val Leu Glu Glu Asn Arg 100 105 110Val Gln Leu Pro Phe Pro Trp Met Lys Ser Thr Lys Ala His Ala Trp 115 120 125Lys Gly Gln Trp Ala Gly Gly Ala Tyr Ala Ala Glu Pro Glu Glu Asn 130 135 140Lys Arg Thr Arg Thr Ala Tyr Thr Arg Ala Gln Leu Leu Glu Leu Glu145 150 155 160Lys Glu Phe Leu Phe Asn Lys Tyr ...
Biobusiness Grant Recipient: John Schorgl, BA. Grant Period: 2017-2018. Award Value: $100,000. Site: Peytant Solutions, Inc.. Project Summary:. Peytant Solutions is developing a human amnion tissue cover scaffold, called the AmnioStent™, for the treatment of malignant airway strictures. The goal of this grant is to conduct sterilization and biocompatibility validation testing of AmnioStent per the FDA Guidance for Premarket Notifications of Esophageal and Tracheal Prostheses and preparation of the FDA 510k application. ...
TY - JOUR. T1 - Fetal rabbit gastric epithelial cells cultured on floating collagen gels. AU - Logsdon, C. D.. AU - Bisbee, C. A.. AU - Rutten, M. J.. AU - Machen, T. E.. PY - 1982/3. Y1 - 1982/3. N2 - Cells were isolated from ∼ 30 d fetal rabbit stomachs and cultured on floating collagen gels. Electron microscopy showed monolayers in which only one cell type persisted. These columnar cells were joined at apical borders by tight junctions and contained an extensive endoplasmic reticular network with an occasional intracellular canaliculus. They also occasionally contained what appeared to be secretory granules (mucus?), and therefore had some characteristics of all the cell types of the intact fetal stomachs, which showed oxyntic, mucous, and undifferentiated cells. In Ussing chambers with Ringers solution on both sides, cultures developed transepithelial potential (potential difference [PD], mV, mucosa ground)=13, resistance (resistance [R], Ω-cm2)=285, and short-circuit current (Isc, ...
Maternal immunoglobulin-G (IgG) is known to be transported across the placental syncytiotrophoblast during the period when the human fetus is incapable of manufacturing these defensive molecules. In this study we investigated the possible role of the amniochorion, that surrounds the amniotic cavity in which the fetus lies, in the transfer of immunoglobulin. Endogenous IgG was localised in the amniochorion by confocal immunofluorescence microscopy and by ultrastructural labelling of ultrathin frozen tissue sections using the protein A-gold technique. Immunoreactivity was identified in the extracellular matrix tissues and necrotic amniotic epithelial cells. Healthy amniotic epithelial cells and cytotrophoblast cells of the chorion laeve were devoid of endogenous IgG. These results suggest a possible nonspecific paracellular transport pathway between cytotrophoblast cells, which may conceivably contribute to the acquisition of passive immunity by the fetus, and offer a rational explanation for the ...
Shortly after the establishment of the circulation of the yolk-sac the folds of the amnion meet and coalesce above the embryo (Fig. 114, 3 and 4, am). After this the inner or true amnion becomes severed from the outer or false amnion, though the two sometimes remain connected by a narrow stalk. The space between the true and false amnion is a continuation of the body cavity. The true amnion consists of a layer of epiblastic epithelium and generally also of somatic mesoblast, while the false amnion consists as a rule of epiblast only; though it is possible that in some cases (the rabbit ?) the mesoblast may be continued along its inner face. Before the two limbs of the amnion are completely severed the epiblast of the umbilical vesicle becomes separated from the subjacent mesoblast and hypoblast of the vesicle (Fig. 114, 3), and, together with the false amnion (sh) with which it is continuous, forms a complete lining for the inner face of the zona radiata. The space between this membrane and the ...
Human amnion as a temporary biologic barrier after hysteroscopic lysis of severe intrauterine adhesions pilot study J Minim Invasive Gynecol. 2010 Sep-Oct;17(5):605-11. doi: [...] ...
Preterm White Matter Brain Injury Is Prevented by Early Administration of Umbilical Cord Blood Cells Researchers assessed the neuroprotective effects of umbilical cord blood cells and optimal administration timing in a fetal sheep model of preterm brain injury. [Exp Neurol] Abstract Amnion-Epithelial-Cell-Derived Exosomes Demonstrate Physiologic State of Cell under Oxidative Stress Investigators determined amnion epithelial cells (AECs) exosome characteristics and their cargo reflected the physiologic status of the cell of origin and suggests that AEC-derived exosomal p38 MAPK plays a major role in determining the fate of pregnancy. [PLoS One] Full Article Reconstitution of Immune Cell in Liver and Lymph Node of Adult- and Newborn-Engrafted Humanized Mice The authors compared reconstitution levels in a variety of immunological tissues, including the lymph node and liver, between mice engrafted intravenously as adults and intrahepatically in newborns. CD34+ cells were enriched from cord blood and ...
2. A Ocular surface reconstruction with allolimbal transplantation and use of two membranes. The inner 9-mm disc acts as a graft and the outer larger membrane as a patch. S. Dua). B The outer membrane has cut through sutures and retracted, exposing the inner membrane. e. 5 Clinical Studies and Outcomes (Definitions of Success and Grading of Disease Severity) A B membrane was intended as a patch and acted as such for the expected duration but the PED did not heal. When the above criteria were applied to 74 procedures involving use of the amniotic membrane, failure of the procedure was observed in 44 % of patients where the membrane was used in the presence of stem cell deficiency, in 33 % of procedures where the membrane was used in the absence of stem cell deficiency and in 44 % of patients where the membrane was used for conjunctival reconstruction. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. References 1. Anderson DF, Ellies P, Pires RT, Tseng SC (2001) Amniotic membrane transplantation for partial ...
Amnion: Amnion,, in reptiles, birds, and mammals, a membrane forming a fluid-filled cavity (the amniotic sac) that encloses the embryo. The amniotic sac and the fluid it contains are
Abstract: The purpose of this study was to investigate the relationship between cell apoptosis and wide-band electromagnetic pulse (WEMP) irradiation parameters and to provide experimental data to establish the biophysics damage model for WEMP. Cell counting kit-8 was used to detect the cell viability changes after WEMP irradiation and flow cytometry assay was used to detect cell apoptosis after irradiation under different WEMP irradiation parameters. Under experimental conditions, field strength and number of pulses were observed to be the main factors that increased the apoptotic rate of human amniotic epithelial cells. Specifically, electric field intensity and number of pulses were determined to be the primary and secondary factors, respectively. The two factors were confirmed to interact with each other, that is, a logistic function was found to exist between the apoptotic rate and electric field strength.. Key words: Wide-band electromagnetic pulse,Apoptosis,Variance analysisCLC, TL71, ...
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Placenta, Cord, Amnion, Chorion Model - $89 A great teaching tool, this model of the placenta, cord, amnion, and chorion is perfect for childbirth education courses. Visit Cascade HealthCare Products secure Website to view our complete selection of educational materials for childbirth educators and professionals or to place an order.
ABSTRACT BACKGROUND: The human placenta-derived amniotic mesenchymal stem cells (hAMSCs) exhibit immunomodulatory, partially pluripotent, and precardiac properties. They express the c-kit cardiac progenitor marker and are readily reprogrammed to iPSCs (MiPSCs). Dual contrast manganese-enhanced MRI (MEMRI) and delayed enhancement MRI (DEMRI) has been developed to evaluate the cardiac restorative effects of hAMSCs, c-kit+ hAMSCs, and MiPSCs.. HYPOTHESIS: MEMRI and DEMRI will identify the mechanism of myocardial restoration.. METHODS: SCID-beige mice underwent LAD ligation and received 250,000 cells or normal saline (NS) in the peri-infarct region. The mice were divided into 4 arms: (1) NS (control group, n=7), (2) hAMSC (n=6), (3) c-kit+ hAMSC group (n=2), and (4) MiPSC group (n=3). The hAMSCs were transfected with a luciferase reporter gene. Cardiac MRI measured the scar, viable myocardium, and LVEF at weeks 1, 2, and 4. Bioluminescence imaging (BLI) was also performed and blood was drawn at week ...
One of the most established roles of oxytocin (OT) is in inducing uterine contractions and labour. Apart from contractions, our recent study have shown that OT can also activate pro-inflammatory pathways in both human myometrial and amnion cells which suggests the pro-inflammatory role of OT should be taken into account when developing tocolytics targeting the OT/OT receptor (OT/OTR) system. The OTR antagonist, Atosiban, is currently used therapeutically for the treatment of PTL. We have previously shown that Atosiban fails to inhibit the pro-inflammatory effects of OT in human amnion, and Atosiban alone activates NF-κB and MAPKs thus upregulating downstream pro-labour genes. In contrast to our findings with Atosiban, the presence of the orally active OTR antagonist, Nolasiban, reduced the effect of OT on NF-κB and p38 kinase activation both in myometrial and amnion cells. Consistent with the activation of these inflammatory mediators, OT led to increases in the expression of COX-2 and ...
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Define amnion: a thin membrane forming a closed sac about the embryos or fetuses of reptiles, birds, and mammals and containing the amniotic fluid
The amniotic sac, commonly called the bag of waters, sometimes the membranes, is the sac in which the fetus develops in amniotes. It is a thin but tough transparent pair of membranes that hold a developing embryo (and later fetus) until shortly before birth. The inner of these fetal membranes, the amnion, encloses the amniotic cavity, containing the amniotic fluid and the fetus. The outer membrane, the chorion, contains the amnion and is part of the placenta. On the outer side, the amniotic sac is connected to the yolk sac, the allantois and, via the umbilical cord, to the placenta. Amniocentesis is a medical procedure where fluid from the sac is sampled to be used in prenatal diagnosis of chromosomal abnormalities and fetal infections. The amniotic cavity is the closed sac between the embryo and the amnion, containing the amniotic fluid. The amniotic cavity is formed by the fusion of the parts of the amniotic fold, which first makes its appearance at the cephalic extremity, and subsequently at ...
Research supported by The Eye Cancer Foundation has proved greater efficacy of this new technique in recreating the outer surface of the eye and inner surface of the eyelids. As published in the American Journal of Ophthalmology on November 2018, tumors of the conjunctiva and eyelids were surgically removed, then amniotic membranes from donor human placentas were sewn into the defects to recreate a normal ocular and inner eyelid surface. Thus, amnion can provide a foundational platform for new cells to grow and flourish. In this case series, super thick amniotic membrane grafts (AMGs) were found to facilitate the healing of wounds. How exactly does graft thickness affect the success of treatment? Well, the greater thickness means it is more easily sewn into the affected area, and. also helps the grafts to remain several weeks after placement. Thicker grafts are less likely to tear, rupture, or dissolve during the postoperative period. Most importantly, following treatment with ST-AMG, every ...
In addition to the placenta, umbilical cord and amniotic fluid, the amniotic membrane is emerging as an immensely valuable and easily accessible source of stem and progenitor cells. This concise review will focus on the stem/progenitor cell properties of human amniotic epithelial and mesenchymal stromal cells and evaluate the effects exerted by these cells and the amniotic membrane on tissue inflammation and fibrosis.
View full text. Author: Vácz G, Cselenyák A, Cserép Z, Benkő R, Kovács E, Pankotai E, Lindenmair A, Wolbank S, Schwarz CM, Horváthy DB, Kiss L, Hornyák I, Lacza Z.. Year: 2016. Journal: Interv Med Appl Sci.. Volume: 8. Issue: 4. Pages: H848-H857. doi: 10.1556/1646.8.2016.4.6.. PubMed PMID: 28180006. PubMed Central PMCID: PMC5283775.. Cited: 4. ...
Phase I-II, controlled, randomized, multicenter clinical trial. The total number of patients to be included is 20. In an initial phase, will be included 5 patients sequentially (be subjected to the experimental treatment), with a month and a half safety period between patients.. At random, 5 of these patients will be implanted cornea and 10 control patients will receive an amniotic membrane transplant as conventional treatment of corneal trophic ulcers in advanced stages.. The study population consists of patients with severe corneal pathology (corneal ulcers refractory to conventional trophic treatment or sequels of previous ulcers having stromal fibrosis and / or limbal failure in the same eye) for which, there isnt currently an effective alternative therapeutic. It is estimated that the inclusion period is approximately 36 months, and a follow-up period of 24 months for each patient. Thus the total duration of the study will be about 60 months from the inclusion of the first patient until ...
Chair: J Dart - Moderator: G Grabner. Surgical management of refractory palpebral allergic conjuctivitis. Quresh B Muskati. AMT for unusual corneal deposits in homocystinuria. Srinivas K Rao. Histology of non-extruding OOKP laminae. Christopher Liu. Can we implant our keratoprosthesis for humans as a temporary use? Hiroshi Shiota. Comparison of infection rates in patients following amniotic membrane transplant (AMT) and keratoprosthesis. Eduardo C Alfonso. Endophthalmitis in patients with keratoprosthesis: Incidence, bacterial etiologies and risk factors. Mahnaz Nouri. Osteo-odonto-keratoprosthesis modified: Long-term results in dry eyes. Giancarlo Falcinelli. Retroprosthesis membranes as complications after keratoprosthesis. Stanislav A Yakimenko. Evaluation of bone reduction of the haptic lamina in osteo-odonto-keratoprosthesis by computerized tomography. Josef Stoiber. Management of vitreoretinal complications in eyes with permanent keratoprosthesis. Subhransu Ray. Corneal blindness from ...
Chair: J Dart - Moderator: G Grabner. Surgical management of refractory palpebral allergic conjuctivitis. Quresh B Muskati. AMT for unusual corneal deposits in homocystinuria. Srinivas K Rao. Histology of non-extruding OOKP laminae. Christopher Liu. Can we implant our keratoprosthesis for humans as a temporary use? Hiroshi Shiota Comparison of infection rates in patients following amniotic membrane transplant (AMT) and keratoprosthesis. Eduardo C Alfonso. Endophthalmitis in patients with keratoprosthesis: Incidence, bacterial etiologies and risk factors. Mahnaz Nouri Osteo-odonto-keratoprosthesis modified: Long-term results in dry eyes. Giancarlo Falcinelli Retroprosthesis membranes as complications after keratoprosthesis. Stanislav A Yakimenko. Evaluation of bone reduction of the haptic lamina in osteo-odonto-keratoprosthesis by computerized tomography. Josef Stoiber. Management of vitreoretinal complications in eyes with permanent keratoprosthesis. Subhransu Ray Corneal blindness from ...
Figure 4. CD147 staining of amniotic membrane stromal infiltrating cells in patients with chemical burns. In the severe burns group, there were many CD147-positive cells in the amniotic membrane (A). In the moderate burns group, CD147-positive cells were only observed in the bottom of the amniotic membrane (B). In the control HSK group, the cells of the amniotic membrane were CD147-negative (C). In the amniotic membrane before transplantation, the epithelium cells of the amniotic membrane were CD147-positive (D). Scale bar: 20 μm ...
Supplier, Exporter, Manufacturer & Trading Company of Dry Amniotic Membrane in Delhi, India. You can easily buy dry amniotic membrane online at lowest price.
CLINICALIndicationsIntended for homologous use in acute and chronic wound care.FDARegulated under PHS 361 [21 CFR 1270 & 1271]: Human cells, tissues, and cellular and tissue-based products. Creates a unified registration and listing system for establishments that manufacture HCT/Ps and establishes donor eligibility, current good tissue practice, and other procedures to pre
One hot July evening, I ran down an empty street as the peppery smell of city rain rose up from where the rain fell, spotting the pavement. The sharp scent set me sneezing. Seconds later huge heavy drops began to pelt my head and back. I took shelter beneath an awning and watched the storm through an amnion of water. Ten minutes later the rain ceased, as abruptly as it had started. The temperature dropped a few degrees, and I felt the materiality of weather, impervious to the great constructed landscape. Manhattan was at once sealed, and as I watched filthy rainwater disappear into subway grates and down street drains, as permeable as anything in nature. [p. 62-63] ...
Whether in clinic or as a biologic augmentation during surgery FloGraft is a cryopreserved, injectable amniotic fluid allograft used to promote the development and protection of damaged tissue at the injured site to help drive healing, tissue regeneration and reduce scar tissue formation.. X-Wrap is a chorion-free (preferred in orthopedics) amnion membrane allograft used as a biological wrap or patch at any surgical site often used as a surgical adjunct of orthopedic repairs including shoulder and knee scopes and reconstruction, meniscal repairs, foot and ankle repairs, etc.. APPLIED BIOLOGICS ...
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Compositions having a combination of specific biological components have been found to exert a number of useful effects in mammalian cells, including modulating TGF β signaling, apoptosis, and proliferation of mammalian cells, as well as decreasing inflammation in mice. These components can be obtained commercially, or can be prepared from biological tissues such as placental tissues. Placental amniotic membrane (AM) preparations described herein include AM pieces, AM extracts, AM jelly, AM stroma, and mixtures of these compositions with additional components. The compositions can be used to treat various diseases, such as wound healing, inflammation and angiogenesis-related diseases.
AmnioChor proposes that amniotic membrane of the placenta (AMP) is a paradigm shifter because a single placenta provides multiple doses for now or later, that can be tissue or cellular therapy, can be autologous, matched allogeneic, or allogeneic therapy.
Embry later became the leader of the indie-rock group Amnion, helping write the group's songs. In February 2008, Amnion ... "Amnion". Bandcamp.com. Archived from the original on February 22, 2014. Retrieved February 13, 2014. "Los Angeles Loves ...
It is closest to the mother and physically supports the much thinner amnion. The inner membrane is the amnion. It is in direct ... Chorioamnionitis also known as intra-amniotic infection (IAI) is an inflammation of the fetal membranes (amnion and chorion) ...
This rupture involves only the amnion; the chorion remains intact. Fibrous bands of the ruptured amnion float in the amniotic ... The cause of amnion tearing is unknown and as such there are no known preventative measures. In rare cases, if diagnosed in ...
Dorsal view, with the amnion laid open. X 30. Dorsum of human embryo, 2.11 mm in length. Section through the embryo. Fetus of ... it is situated between the amnion and the chorion and may lie on or at a varying distance from the placenta. As a rule the duct ... about eight weeks, enclosed in the amnion. Magnified a little over two diameters. Model of human embryo 1.3 mm long. Section ...
Dorsal view, with the amnion laid open. X 30. Human embryo of 2.6 mm. Diagram showing later stage of allantoic development with ...
Dorsal view, with the amnion laid open. X 30. Lateral section through the mammalian blastodisc. Mikawa T, Poh AM, Kelly KA, ...
Amnion nodosum is frequently also present (nodules on the fetal surface of the amnion). The use of oligohydramnios as a ... associated with placental insufficiency amnion nodosum; failure of secretion by the cells of the amnion covering the placenta ... Fetal intervention Adeniran AJ, Stanek J (2007). "Amnion nodosum revisited: clinicopathologic and placental correlations". Arch ...
The forewaters are released when the amnion ruptures. This is commonly known as the time when a woman's "water breaks". When ... can also be performed to release the fluid if the amnion has not spontaneously ruptured. Swallowed amniotic fluid creates urine ...
The chorion and the amnion together form the amniotic sac. It is formed by extraembryonic mesoderm and the two layers of ... Choriogenesis Chorioamnionitis, an inflammation of the chorion and amnion, usually due to bacterial infection. Chorionic ... the amnion is in contact with the latter. The trophoblast is made up of an internal layer of cubical or prismatic cells, the ...
Fetus of about 8 weeks, enclosed in the amnion. Magnified a little over two diameters. Picture of freshly delivered placenta ...
Fetus of about eight weeks, enclosed in the amnion. Magnified a little over two diameters Sectional plan of the gravid uterus ...
When spontaneous rupture of the amnion occurs early in the second trimester, the separation of amnion from chorion produces ... Garza A, Cordero JF, Mulinare J (1988). "Epidemiology of the early amnion rupture spectrum of defects". Am J Dis Child. 142: ...
Subamniotic or preplacental hematomas are contained within amnion and chorion. Rare. Most patients with a small subchorionic ...
The word amniomancy comes from the Latin amnion, meaning membrane. The French proverb Il est ne coiffe is thought to originate ...
The amnion (5) aids in osmoregulation and serves as a saltwater reservoir. The yolk sac (2) surrounding the yolk (3) contains ... An amnion, chorion, and allantois are present during embryonic life. The eggshell (1) protects the crocodile embryo (11) and ...
Dorsal view, with the amnion laid open. X 30.. Epithelial-mesenchymal transition was first recognized as a feature of ...
Diagram showing the expansion of amnion and delimitation of the umbilicus. Model of human embryo 1.3 mm. long. Tail end of ... The allantois, along with the amnion and chorion (other extraembryonic membranes), identify humans and other mammals as well as ... Opened uterus with cat fetus in midgestation: 1 umbilicus, 2 amniotic sac (chorion and amnion), 3 allantois, 4 Yolk sac, 5 ...
Soon amniogenic (amnion forming cells) amnioblasts separate from the epiblast and line the amnion, which encloses the amniotic ... The outer membrane, the chorion, contains the amnion and is part of the placenta. On the outer side, the amniotic sac is ... A premature rupture of membranes (PROM) is a rupture of the amnion that occurs prior to the onset of labor. An artificial ... The amniotic cavity is the closed sac between the embryo and the amnion, containing the amniotic fluid. The amniotic cavity is ...
The amnion. *The foetus. *Plastic Surgery. *Panendoscopy (or triple endoscopy) *Combines larynx, esophagogus, and bronchoscopy ...
Pakistan, Indonesia, Bangladesh, and Malaysia use amnion in reconstructive surgery and burns. Body brokers are firms or ...
... and amnion); mouth gingival fibroblasts; and eye (corneal endothelium and keratocytes, trabecular cells, ciliary epithelium, ...
Righteous Philaret the Merciful of Amnia in Asia Minor. OCA - Feasts and Saints. Ὁ Ὅσιος Ἀντώνιος ὁ Νέος. 1 Δεκεμβρίου. ΜΕΓΑΣ ... of Amnia in Asia Minor (792) Saint Anthony the New, monk of Kios in Bithynia (865) Saint Theokletos, Archbishop of Sparta and ...
Her most significant contribution to the research was her research on human amnion cells with Dr. Milan V. Milovanovic and how ... After the 28th passage in human amnion cells, the measles virus was then inoculated into chick embryos. This procedure was key ... In this research, the team was able to propagate the measles virus throughout 28 serial passages in human amnion cells. ... Milan V. Milovanovic and John Enders, Anna Mitus published their research "Cultivation of Measles Virus in Human Amnion Cells ...
Enders A. C., Schlafke S., Hendrickx A. G. (1986). Differentiation of the embryonic disc, amnion, and yolk sac in the rhesus ...
The Amnion, the beings that inhabit Forbidden Space, would have their mutagens turn the entire human race into Amnion ... Through Soar the Amnion hope to capture the crew of Trumpet for study. For that reason they have placed an Amnioni aboard, the ... Soar is a human ship in the Amnion's employ captained by Sorus Chatelaine, a woman who betrayed and scarred Nick as a young man ... When Trumpet and Soar reach the edge of the swarm, then run into Punisher and an Amnion 'defensive' named Calm Horizons heavily ...
Design: Primary human amnion mesenchymal cells and pregnant mice were employed to investigate the effect of Nrf2 on thrombin- ... Design: Primary human amnion mesenchymal cells and pregnant mice were employed to investigate the effect of Nrf2 on thrombin- ... Design: Primary human amnion mesenchymal cells and pregnant mice were employed to investigate the effect of Nrf2 on thrombin- ... Design: Primary human amnion mesenchymal cells and pregnant mice were employed to investigate the effect of Nrf2 on thrombin- ...
ABS is typically caused when there is damage to the amnion, a part of the placenta. Placenta damages has been linked to ...
... the inner forming the amnion, the outer the false amnion or serosa. The space between the amnion and the serosa constitutes the ... For the alien race in Stephen R. Donaldsons The Gap Cycle, see Amnion (Gap Cycle). The amnion is a membrane that closely ... The amnion, along with the chorion, the yolk sac and the allantois form a protective sac around the embryo. In birds, reptiles ... The amnion stems from the extra-embryonic somatic mesoderm on the outer side and the extra-embryonic ectoderm on the inner side ...
Amnion,, in reptiles, birds, and mammals, a membrane forming a fluid-filled cavity (the amniotic sac) that encloses the embryo ... More About Amnion. 5 references found in Britannica articles. animal. *formation and function in embryo* In animal development ... Amnion, in reptiles, birds, and mammals, a membrane forming a fluid-filled cavity (the amniotic sac) that encloses the embryo. ... In development, the amnion arises by a folding of a mass of extra-embryonic tissue called the somatopleure. Lined with ectoderm ...
Amnion nodosum are nodules on the fetal surface of the amnion, and is frequently present in oligohydramnios. The nodules are ... Amnion nodosum is granules on amnion whereas whitish nodules on the cord suggest a candidial infection. Adeniran AJ, Stanek J ( ... Amnion nodosum and oligohydromnios are associated with pulmonary hypoplasia and renal agenesis. ... 2007). "Amnion nodosum revisited: clinicopathologic and placental correlations". Arch Pathol Lab Med. 131 (12): 1829-33. doi: ...
... Murray D. Mitchell,1 Anna P. Ponnampalam,2 ... Murray D. Mitchell, Anna P. Ponnampalam, and Gregory E. Rice, "Epigenetic Regulation of Cytokine Production in Human Amnion and ...
Thus, amnion can provide a foundational platform for new cells to grow and flourish. In this case series, super thick amniotic ...
Amnios Cryopreserved Liquid Amnion by Bone Bank. Manufacturer: Bone Bank Allografts. No description is available for this ...
... of which are attributed to the pre-term premature rupture of amnion. Rupture of amnion is thought to be associated with a ... These data provide an accurate insight into the biomechanical process of amnion rupture and highlight X-ray diffraction as a ... However, there is very little information concerning the detailed organisation of fibrillar collagen in amnion and how this ... Amnion Is the Subject Area "Amnion" applicable to this article? Yes. No. ...
... amnion nodosum explanation free. What is amnion nodosum? Meaning of amnion nodosum medical term. What does amnion nodosum mean? ... Looking for online definition of amnion nodosum in the Medical Dictionary? ... Related to amnion nodosum: breech presentation, Prune belly syndrome. amnion. [am´ne-on] the innermost fetal membrane, which ... amnion nodo´sum a nodular condition of the fetal surface of the amnion, usually appearing near the insertion of the cord; it ...
The amnion is derived from the embryo and forms as early as eight days after fertilization, when the fate of cells has yet to ... A part of the placenta called the amnion, or the outer membrane of the amniotic sac, is comprised of cells that have strikingly ... This may be due to the fact that these amnion-derived cells do not express a certain enzyme, called telomerase, that is ... To this end, they began looking at the viability of amnion as a cell source in late 2001, obtaining discarded placentas from ...
The umbilical cord passes through the amnion to reach the placenta. Within the umbilical cord, the umbilical vein is larger ...
Cells from the epiblast form a membrane called the amnion, within which the embryo and later the fetus develop until birth. ... Amnion and Germ Layers. Select Subtitle Language. 88 languages available. Afrikaans. Albanian (Tosk) [Shqip]. Arabic [عربي]. ...
In vitro treatment of primary human amnion epithelial cells with 40 μM T-oligos ([TTAGGG]2) that mimic telomere fragments, ... In vitro and in situ telomere fragments increased human and murine amnion p38MAPK, senescence and inflammatory cytokines. We ... could induce human and murine amnion cell senescence. In a cross-sectional clinical study, AF telomere fragment concentrations ... Amnion Is the Subject Area "Amnion" applicable to this article? Yes. No. ...
... amnion, and chorion is perfect for childbirth education courses. Visit Cascade HealthCare Products secure Website to view our ... Amnion, Chorion Model - $89 A great teaching tool, this model of the placenta, cord, ... A great teaching tool, this model of the placenta, cord, amnion, and chorion is perfect for childbirth education courses. Fetal ...
In this study, human amnion epithelial cells (hAECs) were derived from amnion obtained from placentas that were discarded after ... Amnion Epithelial Cells: Cell Therapy Going Green! Message Subject. (Your Name) has forwarded a page to you from Science ... Recycling human amnion allows the production of stemlike cells that differentiate into airway epithelium and soothe injured ... Recycling human amnion allows the production of stemlike cells that differentiate into airway epithelium and soothe injured ...
The amnion and chorion ("amnion" hereafter) form a membrane that surrounds the baby during pregnancy and plays an important ... Amnion Epithelial Cells (AECs) are a stem-like cell that can be isolated in large numbers (150 to 200 million per gram) from ... The fetal membranes - amnion and chorion - also contain stem cells and stem cell-like cells that can be isolated and banked for ... The amnion dressings promote cell growth, modulate inflammation and recruit endogenous (local) stem cells to facilitate wound ...
James L. Chen, MD, (San Francisco, CA) presents the case of a midsubstance Achilles repair augmented with Arthrex Amnion™ ...
... who suggested that the bands derived from early amnion rupture, with formation of mesodermal strings and naked chorion, which ...
Thus, this amnion preparation can serve as a bridge to promote axonal regeneration in vivo in damaged adult brain. ... Human amnion membrane serves as a substratum for growing axons in vitro and in vivo ... Human amnion membrane serves as a substratum for growing axons in vitro and in vivo ... Human amnion membrane serves as a substratum for growing axons in vitro and in vivo ...
Amnion refers to a membranous structure which covers and protects the embryo. It forms inside the chorion. The amnion usually ... Radiographic features Ultrasound The amnion can be visu... ... "Amnion" derives from the Greek word "άμνίον", a bowl in which ... Amnion refers to a membranous structure which covers and protects the embryo. It forms inside the chorion. The amnion usually ... The amnion can be visualised in most pregnancies before the 12th week of gestation and appears as a thin membrane separating ...
Procedure: Amnion-Chorion allograft membrane on the root surface of periodontally diseased site. Application of Amnion-Chorion ... The amnion-chorion BioXclude membrane is regulated by the FDA under section 361 of the Public Health Service Act as a Human ... The FDA views using amnion chorion as a wound covering to aid in the healing of tissues throughout the body to be a homologous ... Application of Amnion-Chorion allograft membrane (ACM) on the root surface of periodontally diseased teeth in conjunction with ...
Device: Allogenic amnion chorion membrane Allogenic amnion chorion membrane (ACM) is a minimally manipulated allograft amnion ... Allogenic amnion chorion membrane (ACM) is a minimally manipulated allograft amnion chorion tissue for use as a wound covering ... Clinical and Histological Outcomes of Allogenic Amnion Chorion Membrane in the Healing of Free Gingival Graft Donor Site. The ... The purpose of this study is to compare an allogenic amnion chorion membrane to a collagen dressing in palatal wound healing ...
Other: Dehydrated Human Amnion/Chorion Membrane Dehydrated human amnion/chorion membrane (dHACM) product and is regulated as a ... Other: Dehydrated Human Amnion/Chorion Membrane Dehydrated human amnion/chorion membrane (dHACM) product and is regulated as a ... Dehydrated Human Amnion Chorion Membrane (dHACM) vs. Control in the Treatment of Partial Thickness Burns.. The safety and ... Dehydrated Human Amnion Chorion Membrane (dHACM) vs. Control in the Treatment of Partial Thickness Burns.. ...
Format VideoCategories proceduresTags vampire amnion, vampire hand lift One thought on "Vampire Hand Lift® with Amnion" * ... ONLY live birth amnion is used (no aborted fetal tissue).. Heres the company that harvest the amnion for us (click),- ... Vampire Hand Lift® with Amnion. Members of the Vampire Facelift® provider group, can login to the members site to see more ... to rejuvenate the hands and face and to receive special pricing from the largest supplier of quality amnion in the U.S. ...
  • Extra-amniotic pregnancy is a rare condition that result from a rupture of the amnion, leading to development of the fetus within the extraembryonic coelom. (wikipedia.org)
  • identifies a potential source of stemlike cells, the human amnion (a membrane that surrounds a fetus and contains the amniotic fluid) for the potential treatment of interstitial lung disease. (sciencemag.org)
  • The amnion can be visualised in most pregnancies before the 12th week of gestation and appears as a thin membrane separating the amniotic cavity, which contains the fetus, from the extra-embryonic coelom and the secondary yolk sac 1 . (radiopaedia.org)
  • amnion membrane around a fetus, said to be originally bowl in which the blood of victims was caught [Liddell & Scott], which is variously said to be of unknown origin, from ame bucket, or a dim. (academic.ru)
  • Caption2 = Human fetus, enclosed in the amnion. (academic.ru)
  • Design and Methods We examined whether amnion-derived adherent cells have the characteristics of mesenchymal stem cells, and whether these adherent cells can support the proliferation of umbilical cord blood-derived lineage-negative and CD34-positive cells (Lin - CD34 + cells) obtained from the same fetus to a greater extent than those derived from other fetuses. (haematologica.org)
  • The new study, performed at the University of Michigan and reported in Nature Materials , provides the first opportunity to study the development of amnion, an essential tissue that envelops and protects the growing fetus. (uofmhealth.org)
  • The properties of Amnion that benefit teh fetus also make it an effective material for providing barrier protection in a number of surgical settings. (sabradent.com)
  • The amnion and amniotic sac expand as the fetus grows, providing a cushion that encases and supports the fetus. (fertilitysmarts.com)
  • The fluid is retrieved from the amnion and contains cells that provide information on the chromosomal structure and development of the fetus. (fertilitysmarts.com)
  • The amnia fluid, which continuously changes every 3 hours 8 times a day, remains constant at a certain temperature, distributes the heat it needs equally to each side in terms of the development of the fetus. (mysweetybaby.com)
  • The amnion is the membrane around the fetus. (lifemapsc.com)
  • Objective: This report tested the hypothesis that oxidative stress can induce fetal amnion cells to produce exosomes, which function as a paracrine intermediary between the fetus and mother and biochemically signal readiness for parturition. (utmb.edu)
  • This study also intends to provide some indications on the in-uiuo transport of cations between mother and fetus across the amnion. (islidedoc.org)
  • A part of the placenta called the amnion, or the outer membrane of the amniotic sac, is comprised of cells that have strikingly similar characteristics to embryonic stem cells, including the ability to express two key genes that give embryonic stem cells their unique capability for developing into any kind of specialized cell, the researchers report. (rxpgnews.com)
  • The MSCs represent less than 1% of the cells present in the placenta, while the average yield of MSCs from chorions and amnions is between 1 to 10 million per gram of tissue. (parentsguidecordblood.org)
  • Vascular endothelial growth factor (VEGF) has been proposed as an important regulator of amniotic fluid absorption across the amnion into the fetal vasculature on the surface of the placenta. (uca.fr)
  • This type of twins commonly has two amnions, one chorion, and one placenta. (umich.edu)
  • Following our optimized protocol, primary yields were 6.3 x 10(6) hAECs and 1.7 x 10(6) hAMSCs per gram amnion. (uzh.ch)
  • To help determine if amnion-derived cells that are delivered directly to tissues would cause tumors, the researchers conducted studies in immune system-deficient mice and found no evidence that tumors had developed seven months after the cells were injected into multiple sites. (rxpgnews.com)
  • Failure to implant and the lack of peripheral tissues like amnion are among the causes of such pregnancy losses. (uofmhealth.org)
  • To confirm that the trigger for embryonic stem cells to form amnion is mechanical, the team made two cell culture plates covered in microscopic silicone bristles, again with the loose gel on top. (uofmhealth.org)
  • We have also demonstrated that Nolasiban treatment alone has no significant stimulatory effect on both the myometrium and amnion. (aspetjournals.org)
  • Both in human myometrium and amnion, OT-induced activation of the canonical NF-κB pathway upregulated key inflammatory labour-associated genes including IL-8, CCL5, IL-6 and COX-2. (nih.gov)
  • 10 µM) suppressed OT-induced NF-κB-p65 phosphorylation, whereas p65-siRNA knockdown reduced basal and OT-induced COX-2 levels in myometrium and amnion. (nih.gov)
  • The simplest interpretation of our in vitro studies is that increased expression of the MMP-9 proenzyme leads to the formation of active enzyme, which, in turn, degrades type IV collagen, leading to amnion cell apoptosis. (levelofhealth.com)
  • The present report documents that type IV collagen is lost from the rat amnion before the onset of labor. (levelofhealth.com)
  • The relative decline in type IV collagen could be the result of diminished synthesis, accumulation of other proteins in the amnion which alters the relative abundance of type IV collagen, increased degradation, or a combination of these processes. (levelofhealth.com)
  • Since amnion type IV collagen levels are reduced to some extent before the rise in MMP-2 and MMP-9 protein and activity, it is likely that these enzymes are not responsible for this phase of the decline. (levelofhealth.com)
  • James L. Chen, MD, (San Francisco, CA) presents the case of a midsubstance Achilles repair augmented with Arthrex Amnion™ matrix. (arthrex.com)
  • Dr. Samimi also demonstrates wrapping the peroneal nerve with Arthrex Amnion™ matrix to help prevent adhesions and scarring. (arthrex.com)
  • Members of the Vampire Facelift® provider group, can login to the member's site to see more specifics of how to use Vampire Amnion™ to rejuvenate the hands and face and to receive special pricing from the largest supplier of quality amnion in the U.S. (vampirefacelift.com)
  • What is Vampire Amnion? (renewmedispa.com)
  • It is screened rigorously to rule out communicable disease, and is sterilized further multiple times prior to release as Vampire Amnion for injection. (renewmedispa.com)
  • Vampire Amnion is an optional "add on" to your platelet rich plasma procedure. (renewmedispa.com)
  • Who may benefit from Vampire Amnion? (renewmedispa.com)
  • Results Culture-expanded amnion-derived adherent cells expressed mesenchymal stem cell markers and HLA-ABC molecules and could differentiate into osteoblasts, adipocytes and chondrocyte-like cells, indicating that the cells have the characteristics of mesenchymal stem cells. (haematologica.org)
  • The presence of 2 chorions and amnions at birth confirm that the twins are dichorionic/diamniotic identical twins arising from a single zygote, a relatively rare gestation process (occurring only in approximately 20% of identical twins). (freethesaurus.com)
  • Consequently, they are associated with two amnions, two chorions, and two placentas, which may (65%) or may not (35%) be fused. (umich.edu)
  • The hypothesis of an intrinsic defect of germ plasma put forward by Streeter to explain the abnormalities observed in 'amniotic bands sequence' (ABS) was not supported by Torpin, who suggested that the bands derived from early amnion rupture, with formation of mesodermal strings and naked chorion, which then constricted or adhered to fetal parts. (bmj.com)
  • Eventually, the baby will float inside the balloon-like amnion, which protects it and allows it to grow and develop safely. (study.com)
  • After the fusion of the edges of the amniotic fold, the two layers of the fold become completely separated, the inner forming the amnion, the outer the false amnion or serosa. (wikipedia.org)
  • The amnion usually fuses with the outer chorion by around 14 weeks of gestation. (radiopaedia.org)
  • The inner wall of the fold has the layers reversed, its outer layer is of somatic mesoderm and inner of ectoderm, the two together form the amnion. (yourarticlelibrary.com)
  • There are two sections within the amniotic sac, the outer layer, called the chorion, and the inner layer called the amnion. (fertilitysmarts.com)
  • The amnion is a protective sac that surrounds a baby in the mother's womb. (study.com)
  • London, Jan 8 (ANI): Scientists at the Baylor College of Medicine in Houston, Texas, have shown that egg white can act as a sealant for the amnion , a membrane that surrounds developing foetuses. (freethesaurus.com)
  • Telomere Fragment Induced Amnion Cell Senescence: A Contributor to Parturition? (plos.org)
  • Marker-assisted analysis of six BMP signaling components ( dpp, gbb, scw, tkv, sax, sog ) by RNA interference revealed that both serosa and amnion specification of M. abdita are dependent on BMP activity. (biologists.org)
  • In fact, a number of randomised clinical trials favour the use of amnions in burns for their promotion of wound healing, patient comfort, and reduced need for dressing changes. (parentsguidecordblood.org)
  • The purpose of this study is to compare an allogenic amnion chorion membrane to a collagen dressing in palatal wound healing after harvesting free gingival graft (FGG). (clinicaltrials.gov)
  • Topical administration of cryopreserved living micronized amnion accelerates wound healing in diabetic mice by modulating local microenvironment. (semanticscholar.org)
  • These findings suggest that, although most women who experience pPROM deliver spontaneously within several days, the amnion has the capacity for wound healing in vivo . (frontiersin.org)