Amnion: The innermost membranous sac that surrounds and protects the developing embryo which is bathed in the AMNIOTIC FLUID. Amnion cells are secretory EPITHELIAL CELLS and contribute to the amniotic fluid.Extraembryonic Membranes: The thin layers of tissue that surround the developing embryo. There are four extra-embryonic membranes commonly found in VERTEBRATES, such as REPTILES; BIRDS; and MAMMALS. They are the YOLK SAC, the ALLANTOIS, the AMNION, and the CHORION. These membranes provide protection and means to transport nutrients and wastes.Chorion: The outermost extra-embryonic membrane surrounding the developing embryo. In REPTILES and BIRDS, it adheres to the shell and allows exchange of gases between the egg and its environment. In MAMMALS, the chorion evolves into the fetal contribution of the PLACENTA.Labor, Obstetric: The repetitive uterine contraction during childbirth which is associated with the progressive dilation of the uterine cervix (CERVIX UTERI). Successful labor results in the expulsion of the FETUS and PLACENTA. Obstetric labor can be spontaneous or induced (LABOR, INDUCED).Amniotic Fluid: A clear, yellowish liquid that envelopes the FETUS inside the sac of AMNION. In the first trimester, it is likely a transudate of maternal or fetal plasma. In the second trimester, amniotic fluid derives primarily from fetal lung and kidney. Cells or substances in this fluid can be removed for prenatal diagnostic tests (AMNIOCENTESIS).Parturition: The process of giving birth to one or more offspring.Pregnancy: The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.Amniotic Band Syndrome: A disorder present in the newborn infant in which constriction rings or bands, causing soft tissue depressions, encircle digits, extremities, or limbs and sometimes the neck, thorax, or abdomen. They may be associated with intrauterine amputations.Decidua: The hormone-responsive glandular layer of ENDOMETRIUM that sloughs off at each menstrual flow (decidua menstrualis) or at the termination of pregnancy. During pregnancy, the thickest part of the decidua forms the maternal portion of the PLACENTA, thus named decidua placentalis. The thin portion of the decidua covering the rest of the embryo is the decidua capsularis.Placenta: A highly vascularized mammalian fetal-maternal organ and major site of transport of oxygen, nutrients, and fetal waste products. It includes a fetal portion (CHORIONIC VILLI) derived from TROPHOBLASTS and a maternal portion (DECIDUA) derived from the uterine ENDOMETRIUM. The placenta produces an array of steroid, protein and peptide hormones (PLACENTAL HORMONES).Labor Onset: The beginning of true OBSTETRIC LABOR which is characterized by the cyclic uterine contractions of increasing frequency, duration, and strength causing CERVICAL DILATATION to begin (LABOR STAGE, FIRST ).Fetal Membranes, Premature Rupture: Spontaneous tearing of the membranes surrounding the FETUS any time before the onset of OBSTETRIC LABOR. Preterm PROM is membrane rupture before 37 weeks of GESTATION.Serous Membrane: A thin lining of closed cavities of the body, consisting of a single layer of squamous epithelial cells (MESOTHELIUM) resting on a thin layer of CONNECTIVE TISSUE, and covered with secreted clear fluid from blood and lymph vessels. Major serous membranes in the body include PERICARDIUM; PERITONEUM; and PLEURA.Cellulose, Oxidized: A cellulose of varied carboxyl content retaining the fibrous structure. It is commonly used as a local hemostatic and as a matrix for normal blood coagulation.

Prostaglandin endoperoxide H synthase mRNA expression in the human amnion and decidua during pregnancy and in the amnion at preterm labour. (1/868)

We have examined the expression of prostaglandin endoperoxide H synthase (PGHS) isoenzymes in the amnion and the decidua during gestation, and the abundance of PGHS mRNA in the amnion at idiopathic preterm labour. PGHS-1 and -2 mRNA abundance in the amnion, determined with ribonuclease protection assays, was significantly (P< 0.05) higher at term than earlier during pregnancy. In contrast, neither PGHS-1 and -2 mRNA values, nor PGHS-specific activity, measured with a cell-free assay, was different in the decidua at term as compared to earlier gestational ages. In individual term patients, PGHS-2 mRNA values in the amnion were positively correlated with PGHS-2 mRNA values in the chorion laeve. PGHS-1 and -2 mRNA abundance was higher (P < 0.05) in the amnion after idiopathic preterm labour than in the absence of labour at the same gestational age (28-35 weeks). Thus, PGHS-1 and -2 are induced in the amnion at term. Furthermore, amniotic PGHS-2 changes in co-ordination with PGHS-2 concentrations in the chorion laeve. PGHS is not induced in the decidua at term. Increased amniotic PGHS expression may contribute to the enhanced intrauterine prostaglandin synthesis before term labour. Both PGHS isoenzymes may participate in the increase of PGHS activity in the amnion at preterm birth.  (+info)

First-trimester cord entanglement in monoamniotic twins. (2/868)

OBJECTIVE: Monoamniotic twinning occurs in only 1% of twin pregnancies, but carries a high perinatal mortality rate. Early and reliable diagnosis is essential if attempts are to be made to reduce the complication rate. We report color Doppler demonstration of cord entanglement in the first trimester, which is diagnostic of monoamnionicity. METHODS: Two patients with twin pregnancies were examined in the first trimester with pulsed and color Doppler insonation of their umbilical arteries. RESULTS: Cord entanglement was suspected and proved by demonstrating differing fetal heart rate patterns in the same direction on umbilical artery Doppler analysis of a common mass of cord vessels. Following appropriate counselling, medical amnioreduction was induced at 20 weeks of gestation to reduce fetal movements and worsening cord entanglement. Delivery was by elective Cesarean section at 32 weeks' gestation and monoamnionicity was confirmed. CONCLUSION: We report a new sign for the demonstration of monoamnionicity in twin pregnancies in the first trimester. This should improve the reliability of early diagnosis, but further studies are required to confirm that, if cord entanglement occurs, it is usually present by the end of the first trimester.  (+info)

Tissue plasminogen activator and its receptor in the human amnion, chorion, and decidua at preterm and term. (3/868)

The plasminogen activator system consists of two proteins: tissue plasminogen activator (tPA) and urokinase plasminogen activator (uPA), which act upon their specific receptors to generate plasmin from plasminogen located on the cell surface. Plasmin then acts directly and indirectly to degrade the components of the extracellular matrix (ECM). This process is likely to be important in the normal turnover of the ECM of fetal membranes and in its premature weakening in preterm premature rupture of the fetal membranes. Quantitative Northern analysis and in situ hybridization have shown that the decidua expresses mRNA for tPA. However, the immunolocalized tPA protein was most strongly associated with the amnion and chorion, as was its receptor annexin II, suggesting that the amnion and chorion are the targets for decidual tPA. At term, decidual tPA expression was unaffected by labor, and the tPA receptor was elevated both before and after labor. At preterm, the converse was found: decidual tPA expression was significantly (p < 0. 05) up-regulated by labor, but the tPA receptor was not. The results suggest that the generation of plasmin at term would be controlled by an increased concentration of the tPA receptor in the amnion and chorion, whereas at preterm a pathological increase in plasmin would be generated by an overexpression of tPA, initiated by labor.  (+info)

Conjunctival epithelial cell differentiation on amniotic membrane. (4/868)

PURPOSE: Amniotic membrane (AM)-reconstructed conjunctival surfaces recover the normal epithelial phenotype with a significantly higher cell density than the control. The present study was undertaken to examine how AM modulates rabbit conjunctival epithelial cell differentiation. METHODS: Rabbit conjunctival epithelial cells (RCEs) were cultured on the basement membrane side of dispase-pretreated AM, with or without seeding rabbit conjunctival fibroblasts (RCFs) on the stromal side. After 7 to 12 days, half of the cultures were raised to the air-liquid interface, and the remainder stayed submerged. A small group of air-lifted cultures containing RCFs was treated with retinoic acid. After 1, 2, and 4 weeks, cultures were terminated and processed for immunostaining with antibodies directed against distinct types of mucins (SMC and AM3), glycocalyx (AMEM2), keratin K3 (AE5), and K12 (AK2). Additionally, western blot analysis was performed for K3 keratin expression. Ultrastructural changes were evaluated by transmission electron microscopy. RESULTS: In general, RCEs grown on AM were uniformly small, negative to AE5 and AK2 antibodies, and positive to AMEM2 and ASPG1 antibodies. Epithelial stratification and cell polarity with prominent microvilli, tight junctions, and hemidesmosomes were more pronounced in air-lifted cultures. RCEs cocultured with RCFs showed scattered AM3-positive goblet cells, which were not increased by retinoic acid. CONCLUSIONS: RCEs cultured on AM primarily exhibit a nongoblet conjunctival epithelial phenotype. Epithelial stratification and cell polarity, features essential for epithelial differentiation, are promoted by air-lifting. This culture model will be useful for studying how growth and differentiation of conjunctival epithelial cells can be modulated further.  (+info)

Expression of cyclo-oxygenase types-1 and -2 in human fetal membranes throughout pregnancy. (5/868)

Human labour is associated with increased prostaglandin synthesis within the fetal membranes. We have studied the expression of the two isoforms of the central prostaglandin synthetic enzyme, cyclo-oxygenase (COX-1 and COX-2), in human fetal membranes throughout pregnancy, at mRNA, protein and activity levels. COX-1 mRNA expression was low in human amnion and chorion-decidua and did not change with gestational age. COX-2 mRNA expression in fetal membranes increased with gestational age, with significant up-regulation prior to the onset of labour and in association with labour. Protein concentrations of COX-1 did not change, whilst concentrations of COX-2 increased from the first to the third trimester. COX activity increased with gestational age and in association with labour, although prostaglandin production in fetal membranes collected after labour was reduced, suggesting reduced substrate supply. These data suggest that it is up-regulation of COX-2, rather than of COX-1, which mediates increased prostaglandin synthesis within the fetal membranes at term. Much of the increase in COX-2 expression precedes the onset of labour, suggesting that it is a cause, rather than a consequence, of labour.  (+info)

Amnion-derived cells express intercellular adhesion molecule-1: regulation by cytokines. (6/868)

We have examined the expression of the intercellular adhesion molecule-1 (ICAM-1) mRNA in primary and established amnion-derived cell cultures and regulation of this expression by tumour necrosis factor-alpha (TNF-alpha) and interleukin (IL)-1beta. TNF-alpha (50 ng/ml) and IL-1beta (1.0 ng/ml) induced 18- and 11-fold increases respectively in expression of the ICAM-1 mRNA in WISH cells (an amnion epithelium-derived cell line). The increase was detectable within one hour of treatment and peaked by two hours. The protein synthesis inhibitor, cycloheximide (10 microg/ml) did not inhibit this induction. Increased levels of ICAM-1 protein were detected in the cells within 4 h after initiation of treatment with either cytokine. By 16 h of treatment with IL-1beta or TNF-alpha ICAM-1 reached 40 and 73 pg/microg cellular protein, representing 6- and 11-fold stimulations respectively. In primary amnion cells, basal expression of ICAM-1 mRNA was undetectable. However, TNF-alpha (50 ng/ml) induced ICAM-1 mRNA within two hours, peak expression being reached between four and eight hours after initiation of treatment. The present report demonstrates for the first time that amnion derived cells can express ICAM-1 and, further, that this expression is regulated by pro-inflammatory cytokines. This has implications for the amnion as a possible source for soluble ICAM-1, for this gene product as a marker for preterm labour, and for participation of the amnion, additional to its reported secretory role, in inflammatory processes of the fetal membranes.  (+info)

Oxytocin receptor expression in human term and preterm gestational tissues prior to and following the onset of labour. (7/868)

Oxytocin receptor (OTR) mRNA expression has previously been demonstrated in human myometrium, decidua, chorion and amnion but the effect of gestational age and the onset of labour has not been determined in these individual tissues. Spatial OTR mRNA expression was examined by in situ hybridization and ligand binding was confirmed using autoradiography with the iodinated oxytocin antagonist d(CH2)5[Tyr(Me)2,Thr4,Tyr-NH29]-vasotocin (125I-OTA). Tissue was collected at term (>37 weeks of gestation) or preterm (24-36 weeks of gestation) caesarean section and classified as labour (contractions every 5 min associated with cervical dilatation) or non-labour. OTR mRNA expression was measured as optical density units from autoradiographs. There was a highly significant (P<0.001) effect of tissue type on expression of OTR mRNA with expression greatest in myometrium, low in decidua and chorion and not detected in placenta. Similar results were obtained with the 125I-OTA-binding studies, indicating that the message was translated. Amnion had an apparently high level of both hybridization and 125I-OTA binding in some samples, but a lack of specificity prevented quantification of the signal in this tissue type. Term myometrium (labour and non-labour) had significantly higher (P<0.01) OTR mRNA expression than preterm myometrium, but there was no further increase in mRNA concentration associated with labour onset. In contrast, 125I-OTA binding in myometrium was already high at 33 weeks and did not increase further either later in pregnancy or with labour. In decidua there was no effect of gestational age or labour onset on OTR mRNA expression or 125I-OTA binding. In summary, OTR mRNA expression in the myometrium increased in late pregnancy whereas decidual expression was much lower and did not rise at term.  (+info)

Human RNA-specific adenosine deaminase ADAR1 transcripts possess alternative exon 1 structures that initiate from different promoters, one constitutively active and the other interferon inducible. (8/868)

RNA-specific adenosine deaminase (ADAR1) catalyzes the deamination of adenosine to inosine in viral and cellular RNAs. Two size forms of the ADAR1 editing enzyme are known, an IFN-inducible approximately 150-kDa protein and a constitutively expressed N-terminally truncated approximately 110-kDa protein. We have now identified alternative exon 1 structures of human ADAR1 transcripts that initiate from unique promoters, one constitutively expressed and the other IFN inducible. Cloning and sequence analyses of 5'-rapid amplification of cDNA ends (RACE) cDNAs from human placenta established a linkage between exon 2 of ADAR1 and two alternative exon 1 structures, designated herein as exon 1A and exon 1B. Analysis of RNA isolated from untreated and IFN-treated human amnion cells demonstrated that exon 1B-exon 2 transcripts were synthesized in the absence of IFN and were not significantly altered in amount by IFN treatment. By contrast, exon 1A-exon 2 transcripts were IFN inducible. Transient transfection analysis with reporter constructs led to the identification of two functional promoters, designated PC and PI. Exon 1B transcripts were initiated from the PC promoter whose activity in transient transfection reporter assays was not increased by IFN treatment. The 107-nt exon 1B mapped 14.5 kb upstream of exon 2. The 201-nt exon 1A that mapped 5.4 kb upstream of exon 2 was initiated from the interferon-inducible PI promoter. These results suggest that two promoters, one IFN inducible and the other not, initiate transcription of the ADAR1 gene, and that alternative splicing of unique exon 1 structures to a common exon 2 junction generates RNA transcripts with the deduced coding capacity for either the constitutively expressed approximately 110-kDa ADAR1 protein (exon 1B) or the interferon-induced approximately 150-kDa ADAR1 protein (exon 1A).  (+info)

*Congenital amputation

Amnion ruptures can be caused by: teratogenic drugs (e.g. thalidomide, which causes phocomelia), or environmental chemicals ... One common cause is amniotic band syndrome, which occurs when the inner fetal membrane (amnion) ruptures without injury to the ... fibrous bands of the amnion that constrict foetal limbs to such an extent that they fail to form or actually fall off due to ... outer membrane (chorion). Fibrous bands from the ruptured amnion float in the amniotic fluid and can get entangled with the ...

*Amniotic sac

Soon amniogenic (amnion forming cells) amnioblasts separate from the epiblast and line the amnion, which encloses the amniotic ... The outer membrane, the chorion, contains the amnion and is part of the placenta. On the outer side, the amniotic sac is ... A premature rupture of membranes (PROM) is a rupture of the amnion that occurs prior to the onset of labor. An artificial ... The amniotic cavity is the closed sac between the embryo and the amnion, containing the amniotic fluid. The amniotic cavity is ...

*Amnion

... the inner forming the amnion, the outer the false amnion or serosa. The space between the amnion and the serosa constitutes the ... For the alien race in Stephen R. Donaldson's The Gap Cycle, see Amnion (Gap Cycle). The amnion is a membrane that closely ... The amnion, along with the chorion, the yolk sac and the allantois form a protective sac around the embryo. In birds, reptiles ... The amnion stems from the extra-embryonic somatic mesoderm on the outer side and the extra-embryonic ectoderm on the inner side ...

*Amnion nodosum

... are nodules on the fetal surface of the amnion, and is frequently present in oligohydramnios. The nodules are ... Amnion nodosum is granules on amnion whereas whitish nodules on the cord suggest a candidial infection. Adeniran AJ, Stanek J ( ... Amnion nodosum and oligohydromnios are associated with pulmonary hypoplasia and renal agenesis. ... 2007). "Amnion nodosum revisited: clinicopathologic and placental correlations". Arch Pathol Lab Med. 131 (12): 1829-33. doi: ...

*Amnion (Gap Cycle)

The Amnion do not appear to have individuals in the human sense of the word, or if so, they do not show it. The only Amnion ... It is suggested that the major instinct of the Amnion is the expansion and propagation of the Amnion race. Milos Taverner - ... The Amnion wish to study the effects of the zone implant on Morn's son (Davies) and demand that Nick return him to them, but ... The Amnion expose Morn's deceptions, by informing Nick both that Morn's son is not Nick's son, and that Morn has a zone implant ...

*Maria of Amnia

... (c. 770 - after 823) was the first Empress consort of Constantine VI of the Byzantine Empire. She was a ... Maria was born in Amnia, Paphlagonia. In 788, Maria was one of thirteen candidates in the earliest recorded bride-show. The ... Maria of Amnia, to Constantine VI, the son of the empress Eirene, in 788." Cawley, Charles, BYZANTIUM: Konstantinos VI died 797 ...

*Aaron Embry

Embry later became the leader of the indie-rock group Amnion, helping write the group's songs. In February 2008, Amnion ... "Amnion". Bandcamp.com. Archived from the original on February 22, 2014. Retrieved February 13, 2014. "Los Angeles Loves ...

*Chorioamnionitis

It is closest to the mother and physically supports the much thinner amnion. The inner membrane is the amnion. It is in direct ... Chorioamnionitis also known as intra-amniotic infection (IAI) is an inflammation of the fetal membranes (amnion and chorion) ...

*Amniotic band constriction

This rupture involves only the amnion; the chorion remains intact. Fibrous bands of the ruptured amnion float in the amniotic ... The cause of amnion tearing is unknown and as such there are no known preventative measures. In rare cases, if diagnosed in ...

*Yolk sac

Dorsal view, with the amnion laid open. X 30. Dorsum of human embryo, 2.11 mm in length. Section through the embryo. Fetus of ... it is situated between the amnion and the chorion and may lie on or at a varying distance from the placenta. As a rule the duct ... about eight weeks, enclosed in the amnion. Magnified a little over two diameters. Model of human embryo 1.3 mm long. Section ...

*Body-stalk

Dorsal view, with the amnion laid open. X 30. Human embryo of 2.6 mm. Diagram showing later stage of allantoic development with ...

*Primitive streak

Dorsal view, with the amnion laid open. X 30. Lateral section through the mammalian blastodisc. Mikawa T, Poh AM, Kelly KA, ...

*Oligohydramnios

Amnion nodosum is frequently also present (nodules on the fetal surface of the amnion). The use of oligohydramnios as a ... associated with placental insufficiency amnion nodosum; failure of secretion by the cells of the amnion covering the placenta ... Fetal intervention Adeniran AJ, Stanek J (2007). "Amnion nodosum revisited: clinicopathologic and placental correlations". Arch ...

*Amniotic fluid

The forewaters are released when the amnion ruptures. This is commonly known as the time when a woman's "water breaks". When ... can also be performed to release the fluid if the amnion has not spontaneously ruptured. Swallowed amniotic fluid creates urine ...

*Chorion

The chorion and the amnion together form the amniotic sac. It is formed by extraembryonic mesoderm and the two layers of ... Choriogenesis Chorioamnionitis, an inflammation of the chorion and amnion, usually due to bacterial infection. Chorionic ... the amnion is in contact with the latter. The trophoblast is made up of an internal layer of cubical or prismatic cells, the ...

*Placenta

Fetus of about 8 weeks, enclosed in the amnion. Magnified a little over two diameters. Picture of freshly delivered placenta ...

*Umbilical cord

Fetus of about eight weeks, enclosed in the amnion. Magnified a little over two diameters Sectional plan of the gravid uterus ...

*Constriction ring syndrome

When spontaneous rupture of the amnion occurs early in the second trimester, the separation of amnion from chorion produces ... Garza A, Cordero JF, Mulinare J (1988). "Epidemiology of the early amnion rupture spectrum of defects". Am J Dis Child. 142: ...

*Chorionic hematoma

Subamniotic or preplacental hematomas are contained within amnion and chorion. Rare. Most patients with a small subchorionic ...

*Amniomancy

The word amniomancy comes from the Latin amnion, meaning membrane. The French proverb Il est ne coiffe is thought to originate ...

*Reptile

The amnion (5) aids in osmoregulation and serves as a saltwater reservoir. The yolk sac (2) surrounding the yolk (3) contains ... An amnion, chorion, and allantois are present during embryonic life. The eggshell (1) protects the crocodile embryo (11) and ...

*Allantois

Diagram showing the expansion of amnion and delimitation of the umbilicus. Model of human embryo 1.3 mm. long. Tail end of ... The allantois, along with the amnion and chorion (other extraembryonic membranes), identify humans and other mammals as well as ... Opened uterus with cat fetus in midgestation: 1 umbilicus, 2 amniotic sac (chorion and amnion), 3 allantois, 4 Yolk sac, 5 ...

*Tissue bank

Pakistan, Indonesia, Bangladesh, and Malaysia use amnion in reconstructive surgery and burns. Body brokers are firms or ...

*Prostaglandin EP3 receptor

... and amnion); mouth gingival fibroblasts; and eye (corneal endothelium and keratocytes, trabecular cells, ciliary epithelium, ...

*December 1 (Eastern Orthodox liturgics)

Righteous Philaret the Merciful of Amnia in Asia Minor. OCA - Feasts and Saints. Ὁ Ὅσιος Ἀντώνιος ὁ Νέος. 1 Δεκεμβρίου. ΜΕΓΑΣ ... of Amnia in Asia Minor (792) Saint Anthony the New, monk of Kios in Bithynia (865) Saint Theokletos, Archbishop of Sparta and ...
Purpose: : To evaluate the efficacy of early sutureless amniotic membrane transplantation in the management of severe bacterial keratitis to reduce pain, inflammation, and haze, and to promote healing. Methods: : A non-comparative case series including 3 eyes of 3 consecutive patients with severe bacterial keratitis exhibiting persistent epithelial defect/ulcer were retrospectively reviewed following early sutureless amniotic membrane transplantation via ProKeraTM together with selective topical antibiotics and preservative-free steroid. Pain relief, inflammation, haze, and corneal epithelial healing were monitored. Results: : ProKeraTM was inserted once in one eye, twice in the other two eyes. Pain was significantly relieved, and inflammation was markedly reduced in all cases. The corneal epithelial defect and stromal ulceration rapidly healed while visual acuity improved in two out of the 3 eyes. Conclusions: : Temporary sutureless amniotic membrane transplantation via ProKeraTM allows easy ...
Purpose: : To examine the characteristics of iris pigment epithelial (IPE) cells cultured on amniotic membrane (AM) and to determine whether subretinal transplantation of this IPE cell sheet can protect photoreceptor cell loss in dystrophic Royal College of Surgeons (RCS) rats. Methods: : Human and rat IPE cells were cultured on the basement membrane side of dispase treated AM. After 2 weeks of seeding, the expression of several genes participate in the function of differentiated retinal pigment epithelial (RPE) cells was evaluated using real time PCR and Western blotting. Ultrastructural changes were evaluated using transmission electron microscopy. The IPE cell sheet cultured on AM was transplanted into the subretinal space of 4-week-old RCS rats, and the eyes were analyzed histologically at 12 weeks after grafting. The effect of transplantation of IPE cell sheet on the visual function was estimated by optokinetic reflex. Results: : IPE cells cultured on AM exhibited ultrastructural epithelial ...
Human amniotic membrane that has been processed and sterilised by gamma irradiation is widely used as a biological dressing in surgical applications. The morphological structure of human amniotic membrane was studied under scanning electron microscop
Amniotic membrane graft to conjunctival flap in treatment of non-viral resistant infectious keratitis: a randomised clinical study ...
Purpose: To evaluate amniotic membrane (AM) tissue morphology and corneal epithelial healing in human eyes after amniotic membrane transplantation (AMT), using laser scanning in vivo confocal microscopy (IVCM). Patients and methods: Twenty eyes of twenty patients, treated with single layer epithelial side up AMT for chemical burns in the acute stage (n=8) and persistent corneal epithelial defect (n=12) were studied by serial IVCM post AMT until complete re-epithelization. Changes in morphology of transplanted amniotic tissue and healing corneal epithelium were noted. AM and corneal epithelial cell density was calculated using image analysis software. Results: IVCM enabled visualisation of transplanted AM and of regenerating epithelial cells under the AM. The mean AM epithelial cell density, one day after transplant, was 4613 ± 380 cell/mm2. Average AM epithelial thickness was 35 ± 4 μm, while AM stromal thickness was 116 ± 31 μm. The amniotic stroma appeared to be composed of a superficial ...
Objective: To evaluate the use of hypothermically stored human amniotic membrane for cartilage repair in adult sheep. Studies show that human amniotic membrane contains pluripotent mesenchymal stem cells that can be influenced to produce chondrocytes. It is unknown if human amniotic cells can produce hyaline-like cartilage. This study evaluates the use of hypothermically stored amniotic membrane (HSAM) to fill chondral defects in a sheep model. We hypothesized HSAM would fill defects with hyaline-like cartilage with chondrocytes populating the matrix. One sheep was used as a control, and four sheep received amniotic membrane. Two of these sheep were used as a normal control comparison. A 1 cm2 defect was created on the trochlear grove in all specimens. Each membrane was sized and laid over with the stromal layer facing the subchondral bone and covered with Fibrin sealant. The knees were harvested at five months and underwent morphological, histological, and immunohistological evaluation based on the
Amniotic Tissue Product and VA Product Literature. AMNIOEXCEL® is a dehydrated human amnion-derived tissue allograft with intact extracellular matrix that provides structural tissue to advance soft tissue repair, replacement and reconstruction.
A reconstituted collagen membrane from human amnion has been investigated as a source of collagen matrix, which could be used as a substratum for culturing human fibroblasts. The suitability of pepsin-solubilized reconstituted human amniotic membrane, before and after cross-linking with chitosan, as a dermal matrix for culturing fibroblast was assessed by morphologic, physicochemical, cytotoxic and histochemical methods. Measurement of thermodynamic behaviour, by differential scanning calorimetric (DSC) and thermogravimetric analysis (TGA), and tensile strength suggested that the cross-linked membrane had sufficient elasticity to serve as an efficient dermal substrate for in vitro culture of fibroblasts. Fibroblasts cultured on the chitosan cross-linked collagen membrane had good adherence, retaining their morphology as indicated by microscopic analysis. Proliferation of fibroblasts, observed on this membrane affirms its non-toxic nature. These results support the application of reconstituted ...
Human amniotic epithelial (HAE) cells have great potential for successful use in cell therapy, since they do not cause acute rejection upon allotransplantation. However, to date, HAE cells have not well been studied. We previously reported that HAE c
References: 1. Niknejad H, Peirovi H, Jorjani M, et al. Properties of the Amniotic Membrane For Potential Use in Tissue Engineering. European Cells and Materials. 2008(15):88-89. 2. Gruss J, Jirsch D. Human amniotic membrane: a versatile wound dressing. CMA Journal.1978(18);1237-1254. 3. Data on File, Nutech Medical. 4. Diaz-Prado A, Rendal-Vasques E, Muinos-Lopez E, et al. Potential use of the human amniotic membrane as a scaffold in articular cartilage repair. Cell Tissue Bank. 2010;11:183-195. 5. Garcia D, Longo U, Vaquero J, Forriol F, Loppini M, Khan W, Denaro V. Amniotic Membrane Transplant for Articular Cartilage Repair: An Experimental Study in Sheep. Current Stem Cell Research & Therapy. 2014;9(6);1-7. 6. Cooke, M, Tan, E, Mandrycky, C, He, H, OConnell, J, Tseng, S, Comparison Of Cryopreserved Amniotic Membrane And Umbilical Cord Tissue With Dehydrated Amniotic Membrane/Chorion Tissue, Journal Of Wound Care 2014, 23(10): 465-76.. Document No.: Affinity-1101 Revision: Initial Release ...
Synonyms for amnions in Free Thesaurus. Antonyms for amnions. 2 synonyms for amnion: amnios, amniotic sac. What are synonyms for amnions?
IL-10 is regarded predominantly as an inhibitor of cell-mediated inflammatory reactions. As such, it has been suggested that IL-10 could have therapeutic potential, including the treatment of preterm labor. Using explant cultures of gestational membranes we have found that IL-10 does indeed exert anti-inflammatory properties in choriodecidua, but that in the adjacent amnion it has remarkable pro-inflammatory actions. Amnion prostaglandin (PG) E 2 production was significantly increased following 24-h treatment with IL-10 (5-100 ng/mL). Production of IL-8 also showed a significant stimulation at 100 ng/mL IL-10. In contrast, choriodecidual production of IL-8 and tumor necrosis factor (TNF)-α was dramatically inhibited by IL-10, confirming the ability of this tissue to exhibit a classical IL-10 response. IL-10 retained its stimulatory actions on amnion in the presence of IL-1β and TNF-α stimulation. These findings suggest that the fetal membranes can exhibit opposing responses to IL-10, ...
Abstract PURPOSE: To determine whether preserved human amniotic membrane can be used as an alternative substrate for treating persistent corneal epithelial defects with sterile ulc..
Results Culture-expanded amnion-derived adherent cells expressed mesenchymal stem cell markers and HLA-ABC molecules and could differentiate into osteoblasts, adipocytes and chondrocyte-like cells, indicating that the cells have the characteristics of mesenchymal stem cells. The Lin-CD34+ cells purified from the frozen umbilical cord blood were strongly positive for HLA-ABC, and contained a large number of hematopoietic stem cells. When the Lin-CD34+ cells were cultured on the autologous (MHC-matched) or MHC-mismatched amnion-derived adherent cells in short-term assays (hematopoietic stem cell-proliferation) and long-term culture-initiating cell assays, greater expansion of the Lin-CD34+ cells was observed in the MHC-matched combination than in MHC-mismatched combinations. The concentration of granulocyte-macrophage colony-stimulating factor in the culture supernatants of the long-term culture-initiating cell assays was significantly higher in the MHC-matched combination than in MHC-mismatched ...
619PRTHuman Immunodeficiency Virus 1Arg Lys Lys Arg Arg Gln Arg Arg Arg1 529PRTHuman Immunodeficiency Virus 2Lys Lys Lys Lys Lys Lys Lys Lys Lys1 539PRTHuman Immunodeficiency Virus 3Arg Arg Arg Arg Arg Arg Arg Arg Arg1 54281PRTHomo sapiens 4Met Asn Gly Glu Glu Gln Tyr Tyr Ala Ala Thr Gln Leu Tyr Lys Asp1 5 10 15Pro Cys Ala Phe Gln Arg Gly Pro Ala Pro Glu Phe Ser Ala Ser Pro 20 25 30Pro Ala Cys Leu Tyr Met Gly Arg Gln Pro Pro Pro Pro Pro Pro His 35 40 45Pro Phe Pro Gly Ala Leu Gly Ala Glu Gln Gly Ser Pro Pro Asp Ile 50 55 60Ser Pro Tyr Glu Val Pro Pro Leu Ala Asp Asp Pro Ala Val Ala His65 70 75 80Leu His His His Leu Pro Ala Gln Leu Ala Leu Pro His Pro Pro Ala 85 90 95Gly Pro Phe Pro Glu Gly Ala Glu Pro Gly Val Leu Glu Glu Asn Arg 100 105 110Val Gln Leu Pro Phe Pro Trp Met Lys Ser Thr Lys Ala His Ala Trp 115 120 125Lys Gly Gln Trp Ala Gly Gly Ala Tyr Ala Ala Glu Pro Glu Glu Asn 130 135 140Lys Arg Thr Arg Thr Ala Tyr Thr Arg Ala Gln Leu Leu Glu Leu Glu145 150 155 160Lys Glu Phe Leu Phe Asn Lys Tyr ...
plane. (a) CFBE41o- cells; (b) hAMSCs- CFBE 1 : 5 ratio; (c) hAMSCs- CFBE 1 : 10 ratio; (d) hAMSCs- CFBE 1 : 15 ratio; (e) hAMSCs alone. The white arrows point to CM-DiI-labelled hAMSCs expressing CFTR on their membrane (green signal). Note in (d) that hAMSCs harbour some CFTR-specific signal in discrete regions under the apical plasma membrane ...
Biobusiness Grant Recipient: John Schorgl, BA. Grant Period: 2017-2018. Award Value: $100,000. Site: Peytant Solutions, Inc.. Project Summary:. Peytant Solutions is developing a human amnion tissue cover scaffold, called the AmnioStent™, for the treatment of malignant airway strictures. The goal of this grant is to conduct sterilization and biocompatibility validation testing of AmnioStent per the FDA Guidance for Premarket Notifications of Esophageal and Tracheal Prostheses and preparation of the FDA 510k application. ...
Maternal immunoglobulin-G (IgG) is known to be transported across the placental syncytiotrophoblast during the period when the human fetus is incapable of manufacturing these defensive molecules. In this study we investigated the possible role of the amniochorion, that surrounds the amniotic cavity in which the fetus lies, in the transfer of immunoglobulin. Endogenous IgG was localised in the amniochorion by confocal immunofluorescence microscopy and by ultrastructural labelling of ultrathin frozen tissue sections using the protein A-gold technique. Immunoreactivity was identified in the extracellular matrix tissues and necrotic amniotic epithelial cells. Healthy amniotic epithelial cells and cytotrophoblast cells of the chorion laeve were devoid of endogenous IgG. These results suggest a possible nonspecific paracellular transport pathway between cytotrophoblast cells, which may conceivably contribute to the acquisition of passive immunity by the fetus, and offer a rational explanation for the ...
Shortly after the establishment of the circulation of the yolk-sac the folds of the amnion meet and coalesce above the embryo (Fig. 114, 3 and 4, am). After this the inner or true amnion becomes severed from the outer or false amnion, though the two sometimes remain connected by a narrow stalk. The space between the true and false amnion is a continuation of the body cavity. The true amnion consists of a layer of epiblastic epithelium and generally also of somatic mesoblast, while the false amnion consists as a rule of epiblast only; though it is possible that in some cases (the rabbit ?) the mesoblast may be continued along its inner face. Before the two limbs of the amnion are completely severed the epiblast of the umbilical vesicle becomes separated from the subjacent mesoblast and hypoblast of the vesicle (Fig. 114, 3), and, together with the false amnion (sh) with which it is continuous, forms a complete lining for the inner face of the zona radiata. The space between this membrane and the ...
Preterm White Matter Brain Injury Is Prevented by Early Administration of Umbilical Cord Blood Cells Researchers assessed the neuroprotective effects of umbilical cord blood cells and optimal administration timing in a fetal sheep model of preterm brain injury. [Exp Neurol] Abstract Amnion-Epithelial-Cell-Derived Exosomes Demonstrate Physiologic State of Cell under Oxidative Stress Investigators determined amnion epithelial cells (AECs) exosome characteristics and their cargo reflected the physiologic status of the cell of origin and suggests that AEC-derived exosomal p38 MAPK plays a major role in determining the fate of pregnancy. [PLoS One] Full Article Reconstitution of Immune Cell in Liver and Lymph Node of Adult- and Newborn-Engrafted Humanized Mice The authors compared reconstitution levels in a variety of immunological tissues, including the lymph node and liver, between mice engrafted intravenously as adults and intrahepatically in newborns. CD34+ cells were enriched from cord blood and ...
Macrophage colony-stimulating factor (CSF-1) increases the tissue invasive potential of the CSF-1 receptor-bearing lung carcinoma cell lines A549 and Calu-1 by increasing the number of endogenously bound urokinase-type plasminogen activators (u-PA)s on these cells. CSF-1, at concentrations which optimize invasion of A549 and Calu-1 cells into human amnion membranes (250 ng/ml), maximally augments the number of u-PA receptors occupied by endogenously produced urokinase. Preincubation of A549 and Calu-1 cells with the anti-u-PA monoclonal antibody MPW5UK (25 µg/ml) or with a 20- to 40-fold stoichiometric excess of fluid phase type 2 plasminogen activator inhibitor abrogates invasiveness, indicating that functionally active cell surface u-PA is essential for tissue invasion. In contrast, fluid phase type 1 plasminogen activator inhibitor (PAI-1, 5-15 units/ml) does not inhibit invasiveness unless preincubated with the amnion membranes. Inhibition of invasion by PAI-1 is abolished by presaturating ...
2. A Ocular surface reconstruction with allolimbal transplantation and use of two membranes. The inner 9-mm disc acts as a graft and the outer larger membrane as a patch. S. Dua). B The outer membrane has cut through sutures and retracted, exposing the inner membrane. e. 5 Clinical Studies and Outcomes (Definitions of Success and Grading of Disease Severity) A B membrane was intended as a patch and acted as such for the expected duration but the PED did not heal. When the above criteria were applied to 74 procedures involving use of the amniotic membrane, failure of the procedure was observed in 44 % of patients where the membrane was used in the presence of stem cell deficiency, in 33 % of procedures where the membrane was used in the absence of stem cell deficiency and in 44 % of patients where the membrane was used for conjunctival reconstruction. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. References 1. Anderson DF, Ellies P, Pires RT, Tseng SC (2001) Amniotic membrane transplantation for partial ...
Amnion: Amnion,, in reptiles, birds, and mammals, a membrane forming a fluid-filled cavity (the amniotic sac) that encloses the embryo. The amniotic sac and the fluid it contains are
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Placenta, Cord, Amnion, Chorion Model - $89 A great teaching tool, this model of the placenta, cord, amnion, and chorion is perfect for childbirth education courses. Visit Cascade HealthCare Products secure Website to view our complete selection of educational materials for childbirth educators and professionals or to place an order.
ABSTRACT BACKGROUND: The human placenta-derived amniotic mesenchymal stem cells (hAMSCs) exhibit immunomodulatory, partially pluripotent, and precardiac properties. They express the c-kit cardiac progenitor marker and are readily reprogrammed to iPSCs (MiPSCs). Dual contrast manganese-enhanced MRI (MEMRI) and delayed enhancement MRI (DEMRI) has been developed to evaluate the cardiac restorative effects of hAMSCs, c-kit+ hAMSCs, and MiPSCs.. HYPOTHESIS: MEMRI and DEMRI will identify the mechanism of myocardial restoration.. METHODS: SCID-beige mice underwent LAD ligation and received 250,000 cells or normal saline (NS) in the peri-infarct region. The mice were divided into 4 arms: (1) NS (control group, n=7), (2) hAMSC (n=6), (3) c-kit+ hAMSC group (n=2), and (4) MiPSC group (n=3). The hAMSCs were transfected with a luciferase reporter gene. Cardiac MRI measured the scar, viable myocardium, and LVEF at weeks 1, 2, and 4. Bioluminescence imaging (BLI) was also performed and blood was drawn at week ...
One of the most established roles of oxytocin (OT) is in inducing uterine contractions and labour. Apart from contractions, our recent study have shown that OT can also activate pro-inflammatory pathways in both human myometrial and amnion cells which suggests the pro-inflammatory role of OT should be taken into account when developing tocolytics targeting the OT/OT receptor (OT/OTR) system. The OTR antagonist, Atosiban, is currently used therapeutically for the treatment of PTL. We have previously shown that Atosiban fails to inhibit the pro-inflammatory effects of OT in human amnion, and Atosiban alone activates NF-κB and MAPKs thus upregulating downstream pro-labour genes. In contrast to our findings with Atosiban, the presence of the orally active OTR antagonist, Nolasiban, reduced the effect of OT on NF-κB and p38 kinase activation both in myometrial and amnion cells. Consistent with the activation of these inflammatory mediators, OT led to increases in the expression of COX-2 and ...
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The technique to harvest the amnion graft and creation of neovagina involves the use of amnion, consent from both, the donor and recipient, testing for infectious diseases, preparation of graft. The timing of Caesaean Section is important to havest a fresh graft and this can be implamted immediately after adequate washing and preparation with normal saline and antiseptic solutions. The graft mold mounting of the graft onto the mole is done prior to the creation of neovagina. The mold is covered with sterile condom and the graft is mounted over it. The space in between the rectum and bladder is infiltrated with Marcaine/ Adrenalin 1:200, diluted into 40mls solution ( N/S). Dissection start at the vestibule of vagina and the space is created using sharp and blunt dissection (using fingers & retractors). There can be excessive bleeing from the inferior rectal vessels and adequate haemostasis is essential prior to the introduction of the graft mold into the newly created vaginal space. ...
Define amnion: a thin membrane forming a closed sac about the embryos or fetuses of reptiles, birds, and mammals and containing the amniotic fluid
The amniotic sac, commonly called the bag of waters, sometimes the membranes, is the sac in which the fetus develops in amniotes. It is a thin but tough transparent pair of membranes that hold a developing embryo (and later fetus) until shortly before birth. The inner of these fetal membranes, the amnion, encloses the amniotic cavity, containing the amniotic fluid and the fetus. The outer membrane, the chorion, contains the amnion and is part of the placenta. On the outer side, the amniotic sac is connected to the yolk sac, the allantois and, via the umbilical cord, to the placenta. Amniocentesis is a medical procedure where fluid from the sac is sampled to be used in prenatal diagnosis of chromosomal abnormalities and fetal infections. The amniotic cavity is the closed sac between the embryo and the amnion, containing the amniotic fluid. The amniotic cavity is formed by the fusion of the parts of the amniotic fold, which first makes its appearance at the cephalic extremity, and subsequently at ...
In addition to the placenta, umbilical cord and amniotic fluid, the amniotic membrane is emerging as an immensely valuable and easily accessible source of stem and progenitor cells. This concise review will focus on the stem/progenitor cell properties of human amniotic epithelial and mesenchymal stromal cells and evaluate the effects exerted by these cells and the amniotic membrane on tissue inflammation and fibrosis.
Zheng XY, Wan QQ, Zheng CY, Zhou HL, Dong XY, Deng QS, Yao H, Fu Q, Gao M, Yan ZJ, Wang SS, You Y, Lv J, Wang XY, Chen KE, Zhang MY, Xu RX. Amniotic Mesenchymal Stem Cells Decrease Aβ Deposition and Improve Memory in APP/PS1 Transgenic Mice ...
Phase I-II, controlled, randomized, multicenter clinical trial. The total number of patients to be included is 20. In an initial phase, will be included 5 patients sequentially (be subjected to the experimental treatment), with a month and a half safety period between patients.. At random, 5 of these patients will be implanted cornea and 10 control patients will receive an amniotic membrane transplant as conventional treatment of corneal trophic ulcers in advanced stages.. The study population consists of patients with severe corneal pathology (corneal ulcers refractory to conventional trophic treatment or sequels of previous ulcers having stromal fibrosis and / or limbal failure in the same eye) for which, there isnt currently an effective alternative therapeutic. It is estimated that the inclusion period is approximately 36 months, and a follow-up period of 24 months for each patient. Thus the total duration of the study will be about 60 months from the inclusion of the first patient until ...
Chair: J Dart - Moderator: G Grabner. Surgical management of refractory palpebral allergic conjuctivitis. Quresh B Muskati. AMT for unusual corneal deposits in homocystinuria. Srinivas K Rao. Histology of non-extruding OOKP laminae. Christopher Liu. Can we implant our keratoprosthesis for humans as a temporary use? Hiroshi Shiota. Comparison of infection rates in patients following amniotic membrane transplant (AMT) and keratoprosthesis. Eduardo C Alfonso. Endophthalmitis in patients with keratoprosthesis: Incidence, bacterial etiologies and risk factors. Mahnaz Nouri. Osteo-odonto-keratoprosthesis modified: Long-term results in dry eyes. Giancarlo Falcinelli. Retroprosthesis membranes as complications after keratoprosthesis. Stanislav A Yakimenko. Evaluation of bone reduction of the haptic lamina in osteo-odonto-keratoprosthesis by computerized tomography. Josef Stoiber. Management of vitreoretinal complications in eyes with permanent keratoprosthesis. Subhransu Ray. Corneal blindness from ...
Chair: J Dart - Moderator: G Grabner. Surgical management of refractory palpebral allergic conjuctivitis. Quresh B Muskati. AMT for unusual corneal deposits in homocystinuria. Srinivas K Rao. Histology of non-extruding OOKP laminae. Christopher Liu. Can we implant our keratoprosthesis for humans as a temporary use? Hiroshi Shiota Comparison of infection rates in patients following amniotic membrane transplant (AMT) and keratoprosthesis. Eduardo C Alfonso. Endophthalmitis in patients with keratoprosthesis: Incidence, bacterial etiologies and risk factors. Mahnaz Nouri Osteo-odonto-keratoprosthesis modified: Long-term results in dry eyes. Giancarlo Falcinelli Retroprosthesis membranes as complications after keratoprosthesis. Stanislav A Yakimenko. Evaluation of bone reduction of the haptic lamina in osteo-odonto-keratoprosthesis by computerized tomography. Josef Stoiber. Management of vitreoretinal complications in eyes with permanent keratoprosthesis. Subhransu Ray Corneal blindness from ...
Figure 4. CD147 staining of amniotic membrane stromal infiltrating cells in patients with chemical burns. In the severe burns group, there were many CD147-positive cells in the amniotic membrane (A). In the moderate burns group, CD147-positive cells were only observed in the bottom of the amniotic membrane (B). In the control HSK group, the cells of the amniotic membrane were CD147-negative (C). In the amniotic membrane before transplantation, the epithelium cells of the amniotic membrane were CD147-positive (D). Scale bar: 20 μm ...
Supplier, Exporter, Manufacturer & Trading Company of Dry Amniotic Membrane in Delhi, India. You can easily buy dry amniotic membrane online at lowest price.
amnion, ov bl na - vnit n plodov bl na na z rodku suchozemsk ch ivo ich ; vnit n z rode n obal hmyzu. CoJeCo.cz - V eobecn encyklopedie
Whether in clinic or as a biologic augmentation during surgery FloGraft is a cryopreserved, injectable amniotic fluid allograft used to promote the development and protection of damaged tissue at the injured site to help drive healing, tissue regeneration and reduce scar tissue formation.. X-Wrap is a chorion-free (preferred in orthopedics) amnion membrane allograft used as a biological wrap or patch at any surgical site often used as a surgical adjunct of orthopedic repairs including shoulder and knee scopes and reconstruction, meniscal repairs, foot and ankle repairs, etc.. APPLIED BIOLOGICS ...
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Compositions having a combination of specific biological components have been found to exert a number of useful effects in mammalian cells, including modulating TGF β signaling, apoptosis, and proliferation of mammalian cells, as well as decreasing inflammation in mice. These components can be obtained commercially, or can be prepared from biological tissues such as placental tissues. Placental amniotic membrane (AM) preparations described herein include AM pieces, AM extracts, AM jelly, AM stroma, and mixtures of these compositions with additional components. The compositions can be used to treat various diseases, such as wound healing, inflammation and angiogenesis-related diseases.
Objective: Replacement of degenerated RPE cells as a therapeutic modality for AMD requires that the transplanted cells reconstitute the normal subretinal anatomy. Since transplantation of cell suspensions has not resulted in functional improvement, we hypothesize that it will be necessary to transplant cells cultured on a substratum permissive for monolayer formation with correct polarity, thus allowing communication between transplanted cells and the exposed photoreceptors. The biodegradable substrate serves as a temporary Bruchs membrane prosthesis until the transplanted cells re-assemble their native Bruchs membrane. Here we compared in vitro growth characteristics of RPE and IPE cells and in vivo biocompatibility of potential biomaterials.. Methods: ARPE-19, primary bovine RPE and IPE cells cultured on ultrathin (7μm) equine collagen type I membranes, human amniotic membranes, silk membranes and polylactic acid (PLA) electrospun nanofibers were analyzed for growth, viability and ...
AmnioChor has compiled a spreadsheet (available for download) of all tissue products that are currently manufactured from the Amniotic Membrane of the Placenta (AMP).
This procedure reduces scaring, pain, and inflammation. It also improves healing.. To see if this procedure is right for you, please ask your doctor about Amniotic Membrane Allograft technology.. ...
Refusing to limit itself to just one area of regenerative healthcare, The Electrospinning Company is also working in collaboration with the University of Sheffield to develop a simplified, affordable solution for corneal regeneration. This is required when a patient loses the cells that renew the corneal epithelium - the cells at the front of the eye that normally keep the cornea clear and scar-free. Left untreated, a scar-type tissue grows over the eye resulting in pain and eventual loss of vision. There is limited treatment available for this problem around the world due to the cost of the specialist facilities, expensive equipment and high-level of surgical skill required.. The Electrospinning Company has developed biodegradable, sterile electrospun membranes for the project, which can be used as an alternative to the human amniotic membrane. They can be stored for at least a year and are ready for use instantly. The first human pilot testing is forecast to take place sometime in the next ...
MiMedx® is an integrated developer, processor and marketer of patent protected and proprietary regenerative biomaterial products and bioimplants processed from human amniotic membrane and other birth tissues and human skin and bone. This website is not meant as a substitute for professional medical advice.. © 2018 MiMedx Group, Inc. All rights reserved. , 1775 West Oak Commons Ct. NE, Marietta, GA ...
Regenerative Medicine - Surgical Allografts and Amniotic Tissue. New to the UK we offer a range of unique Allografts (human derived) for use in the surgical field. Following long established trends in the US for reconstruction and the use of Amniotic membranes we wanted to bring you the best in these fields.. ...
The simplest interpretation of our in vitro studies is that increased expression of the MMP-9 proenzyme leads to the formation of active enzyme, which, in turn, degrades type IV collagen, leading to amnion cell apoptosis. Alternative interpretations include the possibility that the increased proMMP-9 sequestered endogenous tissue inhibitors of me-talloproteinases, leading to increased activities of […] ...
galectin-1 May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Homodimer. Binds LGALS3BP. Interacts with CD2, CD3, CD4, CD7, CD43 and CD45. Interacts with laminin (via poly-N- acetyllactosamine). Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion. Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells. Note: This description may include information from UniProtKB ...
Many in vivo processes, including morphogenesis or tumour maturation, involve small populations of cells within a spatially restricted region. However, the basic mechanisms underlying the dynamics of confined cell assemblies remain largely to be deciphered and would greatly benefit from well-controlled in vitro experiments. Here we show that confluent epithelial cells cultured on finite population-sized domains, exhibit collective low-frequency radial displacement modes as well as stochastic global rotation reversals. A simple mathematical model, in which cells are described as persistent random walkers that adapt their motion to that of their neighbours, captures the essential characteristics of these breathing oscillations. As these epithelia mature, a tri-dimensional peripheral cell cord develops at the domain edge by differential extrusion, as a result of the additional degrees of freedom of the border cells. These results demonstrate that epithelial confinement alone can induce morphogenesis-like
How does one seek without seeking,. do without doing,. be without being,. ask without asking,. and who is there to ask?. The paradox confounds me.. I stare into the void of my not knowing. until trees grows before my eyes,. grasses sprout, mature, seed and die,. until I become a stone standing there. in the cleft of our formation,. until I forget the I who has asked,. and the stone crumbles into nothingness,. and the questions dissolve at last. into the air that bore them from my lips. as if never asked,. and then….. as if a light sparked. in the dark sea of my presence,. the universe is born,. the caul is broken,. and the unveiled answers are revealed.. for no reason.. Russell Rosander. 2/23/16 Barrra de Navidad, Mx. div.wpmrec2x{max-width:610px;} div.wpmrec2x div.u > div{float:left;margin-right:10px;} div.wpmrec2x div.u > div:nth-child(3n){margin-right:0px;} ...
AUTHORS NOTES: I know what youre thinking. Youre thinking, No, really, this chapter is a mirage. Either that, or youre thinking that you dont even remember this story. ^_~ In any case, I have to thank you so, *so* much for taking the time to read this . I really appreciate it. I know Ive…
Alpha-fetoprotein (AFP) - a protein produced by the fetus that is excreted into the amniotic fluid. Abnormal levels of alpha-fetoprotein may indicate brain or spinal cord defects, multiple fetuses, a miscalculated due date, or chromosomal disorders.. Amniocentesis - a test performed to determine chromosomal and genetic disorders and certain birth defects. The test involves inserting a needle through the abdominal and uterine wall into the amniotic sac to retrieve a sample of amniotic fluid.. Amniotic sac - a thin-walled sac that surrounds the fetus during pregnancy. The sac is filled with amniotic fluid - liquid made by the fetus and the amnion (the membrane that covers the fetal side of the placenta) that protects the fetus from injury and helps to regulate the temperature of the fetus.. E. coli O157:H7 (also called E. coli or Escherichia coli) - species of bacteria found in the intestines of man and healthy cattle; often the cause of urinary tract infections, diarrhea in infants, and wound ...
return to top]. alpha-fetoprotein - a protein produced by the fetus that is excreted into the amniotic fluid. Abnormal levels of alpha-fetoprotein may indicate brain or spinal cord defects, multiple fetuses, a miscalculated due date, or chromosomal disorders.. amniocentesis - a test performed to determine chromosomal and genetic disorders and certain birth defects. The test involves inserting a needle through the abdominal and uterine wall into the amniotic sac to retrieve a sample of amniotic fluid.. amniotic sac - a thin-walled sac that surrounds the fetus during pregnancy. The sac is filled with amniotic fluid (liquid made by the fetus) and the amnion (the membrane that covers the fetal side of the placenta), which protects the fetus from injury and helps to regulate the temperature of the fetus.. anemia - a decrease of red blood cells in the blood.. anus - the opening at the end of the anal canal.. Apgar test - A scoring system to evaluate the condition of the newborn immediately after ...
In medical terminology En Caul Baby Birth is a rare term. It connotes a situation where the baby finds itself in an amniotic sac and this is right after birth. This sac is full of fluid inside the womb where the baby grows and develops. It is also known as membranes as the sac is embedded with a couple of them known as the chorion and amnion. The sac is filled with a plain fluid liquid where the baby moves and floats. This sac tends to break on its own after birth and as per the mother it is known as water breaking. It has to be stated that in this form of birth the baby is born inside the intact membranes.. In certain cases the baby is born with a caul attached to their head or face. Babies who are born in this manner are stated to be the ones born with a shirt. It is harmless and the piece of membrane is being removed by the doctor in an easy manner. Sometimes this form of birth is considered to be frightening for a mother but just consider it as a harmless form of birth.. This form of birth ...
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The close-packed (111) surface of gold is well known to show a 22×√3 reconstruction on single nm lengths with a long-range herringbone pattern on scales of a few hundred nm. Here we investigate the local reconstruction using density functional theory and compare the results to scanning tunneling microscopy experiments. Moreover, we use hydrogen and fluorine as probe atoms to investigate changes in the ability of the Au(111) surface to catalyze the reactions involved in the formation of molecular nanostructures. We find a small variation of the reactivity across different surface sites and link those results to the local coordination environment of the face-centered-cubic (fcc), hexagonal-close-packed (hcp), and ridge regions. Finally, we scrutinize a commonly used approximation in density functional studies, namely that Au(111) is atomically flat and a perfect termination of the fcc lattice.. ...
We present our efforts to build a database of high quality, multiresolution reconstructions of human bone shapes. We state our rationale for using laser scanning as our digitizing process and describe our reconstruction pipeline. Additionally, we describe the semantic and metadata that is kept with each digital sample and our initial efforts to validate our results. A preview of our database is available online and will be openly available for research in anatomy, education, and biomechanical simulation.
Castroviejo dermatome, an electric dermatome used for cutting mucous membrane grafts for the treatment of eyelid and socket deformities and as an adjunct in the removal of tattoos after the initial excision has been done using either the Brown or Padgett dermatomes. It has a tiny cutting head with special blades and skims to control the thickness of the cut. ...
Supportive Structures Certain structures develop to support, maintain, new life throughout pregnancy - Embryonic or fetal membrane (chorion and amnion) - Placenta - Umbilical cord
Linguistically, Amnion is related to Lamb and Cloud. In Hebrew, roots grow from letters and combine to form the larger branches of the linguistic tree. This is called Hebrew morphology. I happen to have a Hebrew Lexicon that I can use to track the progress and meaning of these words as they connect. The words tell a story as they branch. By this branching, we can reveal a mystery. As it turns out, the word LAMB and SHEEP connect throughout the branches of the various root words and tell a starteling story about why we are unable to see beyond the womb of the Earth and the Veil over our temple (Body / Mind). I will travel down the rabbits hole in sequence with commentary ...
When injected intravenously into newborn rats, eight human tissue-cultured cancer cell lines-H.Ep. #2, Detroit 6, J-111, RP 41, RP 212, Adeno Cx 1, Ovary 2, and MAC 21-grew progressively in lung and other organs of more than 50 per cent of the animals and commonly cuased death after 5-8 weeks. Two other human cell lines-the carcinoma H.Ep. #1 and the presumably normal Amnion B-grew less frequently and rarely caused death or illness.. ...
apparently none of you fucks have seen a real delivery.....and this one is fake. abilical card is not right, baby is covered in something not amnionic fluid, and there is nothing on the ground. not even ART well bad art maybe ...
2. Niknejad H, Peirovi H, Jorjani M, Ahmadiani A, Ghanavi J, Selfallan AM. Properties of the amniotic membrane for potential use in tissue engineering. Eur Cell Mater. 2008; 15:88-89.. ...
The lack of Smad5 in mouse embryos results in ectopic Nodal expression and signaling in the anterior part of the amnion, leading ultimately to the formation of an ectopic primitive streak-like structure therein. Before appearance of the clump in the Smad5 mutant amnion, ectopic Nodal localizes in cuboidal amniotic ectoderm at the anterior midline quadrant of the amnion, whereas the remainder of the mutant amniotic ectoderm acquires the characteristic squamous amniotic epithelium architecture. The (1) altered cellular architecture of the amniotic ectoderm in the anterior midline quadrant of the mutant amnion, (2) the ectopic presence of mesoderm inducers herein, (3) the subsequent presence of several mesoderm markers, including Eomes (which is required in the primitive streak region to downregulate E-cadherin and allow EMT) (Arnold et al., 2008), and (4) the altered regionalized localization of E-cadherin and Snail are compatible with the amniotic clump in the Smad5 mutant embryo being an ectopic ...
Methods This retrospective study included 200 patients, above 8 years of age, with clinically diagnosed unilateral total limbal stem cell deficiency due to ocular surface burns treated between 2001 and 2010. A small limbal biopsy was obtained from the unaffected eye. The limbal epithelial cells were expanded ex vivo on human amniotic membrane for 10-14 days using a xeno-free explant culture system. The resulting cultured epithelial monolayer and amniotic membrane substrate were transplanted on to the patients affected eye. Postoperative corneal surface stability, visual improvement and complications were objectively analysed. ...
Aim. This project studies the regeneration of epidermal and dermal components with the use human Whartons Jelly Mesenchymal Cells (WJ-MSCs) on a scaffold of Freeze Dried Human Amniotic Membrane (FD-HAM) and Cryopreserved Human Amniotic Membrane (CP-HAM) in porcine excisional wound model. Methods. Fresh AM was harvested from seronegative term pregnant mothers undergoing elective Caesarean section deliveries under sterile conditions. The membrane was de-cellularised and either cryopreserved (CP-HAM) or freeze dried (FD-HAM) for storage. Commercially available WJ-MSCs were seeded on the different forms of the decellularised membrane. Six, 5×5 cm cutaneous wounds using a dermatome at 0.8mm setting, 2cm apart will be created on the dorsum of 20-25kg Great White pigs on either side of the spine under general anaesthesia. The treatments (Wound control, WJ-MSCs alone, FD-HAM, CP-HAM, FD-HAM + WJ-MSCs, CP-HAM+ WJ-MSCs) were allocated by Latin squares to randomise the application. A barrier dressing ...
Preterm delivery occurs in 10% of all births. It is the major cause of infant death and handicap in the developed countries and accounts for 65% of neonatal deaths and 50% of childhood neurological disabilities. At the end of pregnancy, pro-labour factors begin to mediate remodeling of the cervix resulting in cervical ripening and dilatation, uterine contractility and decidual/fetal membrane activation. The amnion plays an important role in the onset of human labour. It is a major site for prostaglandins (PG) and inflammatory cytokine synthesis which increases both before and during labour. Amnion derived inflammatory cytokines and prostaglandins contribute to the relaxation of the lower uterine segment and to cervical ripening. Oxytocin (OT) and oxytocin receptor (OTR) are classically considered to play a fundamental role in the mechanism of labour as OT stimulates uterine contractions and OTR antagonists are clinically used as tocolytics. The increase in OT and OTR expressions were observed ...
The aim of the present study was to investigate the molecular mechanisms underlying activation of cell death pathways using genome-wide transcriptional analysis in human limbal epithelial cell (HLEC) cultures following conventional hypothermic storage in Optisol-GS. Three-week HLEC cultures were stored in Optisol-GS for 2, 4, and 7 days at 4 °C. Partek Genomics Suite software v.6.15.0422, (Partec Inc., St. Louis, MO, USA) was used to identify genes that showed significantly different (P < 0.05) levels of expression following hypothermic storage compared to non-stored cell sheets. There were few changes in gene expression after 2 days of storage, but several genes were differently regulated following 4 and 7 days of storage. The histone-coding genes HIST1H3A and HIST4H4 were among the most upregulated genes following 4 and 7 days of hypothermic storage. Bioinformatic analysis suggested that these two genes are involved in a functional network highly associated with cell death, necrosis, and
AMT was performed in 24 eyes of 24 patients for erosions without stromal defect (n=6) and ulcers (n=18), which were resistant to medical therapy. All the patients had undergone one (42%) or more PKs prior to AMT. After removal of the epithelium and pannus (if present), one or more layers of AM depending on the depth of the lesion were fixed with interrupted 10-0 nylon suture. Three different surgical techniques were performed: inlay or graft (n=3), overlay or patch (n=5), and sandwich or combination of graft and patch (n=16). Main outcome measures included surgical success (SS), if the epithelium was closed within four weeks after AMT, and recurrence (R), if a new epithelial defect developed during follow-up (15 12 months) after the complete epithelial closure.. ...
Approaches providing the positioning of human amniotic membrane (HAM) within the intra-articular space of arthritic TMJs have never been investigated. This contrasts with the increasing amount of evidence suggesting the potential positive effects of HAM on a number of surgical conditions, even included the interpositional arthroplasty for TMJ ankylosis. Thus, the possible usefulness of HAM to restore joint functions in severely damaged TMJs could be hypothesized. Based on these premises, the clinical research question "Is human amniotic membrane positioning effective to reduce symptoms and restore jaw function in patients with severe inflammatory-degenerative disorders of the temporomandibular joint?" has been addressed by performing a systematic review of the literature ...
Abstract A new method of drying and sterilizing the amnion extends the life of the product to more than nine months and enables it to be stored at room temperature. The bovine amni..
The gestational sac is the large cavity of fluid surrounding the embryo. During early embryogenesis it consists of the extra-embryonic coelom, also called the chorionic cavity. The gestational sac is normally contained within the uterus. It is the only available structure that can be used to determine if an intrauterine pregnancy exists until the embryo is identified. On obstetric ultrasound, the gestational sac is a dark ("anechoic") space surrounded by a white ("hyperechoic") rim. The gestational sac is spherical in shape, and usually located in the upper part of the fundus of the uterus. By approximately 9 weeks of gestational age, the amniotic sac has expanded to occupy the majority of the volume of the gestational sac, eventually expanding to reduce the extraembryonic coelom to a thin layer between the amnion membrane and the mesoderm. By then, the gestational sac is usually simply called the "amniotic sac". During embryogenesis, the extraembryonic coelom (or chorionic cavity) that ...
The human tissue inhibitor of metalloproteinases (TIMP) is a glycoprotein with a molecular weight of 28,000. It appears to be ubiquitous in human mesoderm tissues and has previously been shown to be identical to the collagenase inhibitor isolated from human skin fibroblasts. TIMP inhibits type I- and IV-specific collagenases and other neutral metalloen-doproteinases that may be responsible for the degradation of extracellular matrix in tumor cell metastasis. In this work we have utilized recombinant human TIMP (rTIMP) obtained by expression of its cDNA gene (Carmichael et al., Proc. Natl. Acad. Sci. USA, 83: 2407, 1986). The rTIMP is shown to have similar inhibition properties as natural TIMP against human skin fibroblast collagenase. In an in vitro amnion invasion assay system, rTIMP inhibited the invasion of B16-F10 murine melanoma cells through the human amniotic membrane at an identical concentration to that reported previously for natural TIMP. The mechanism by which rTIMP inhibits amniotic ...
Patient I am an Australian whose daughter contacted Dr. Maskin as soon as we heard about him and that he was performing a procedure for extreme Dry Eye Syndrome, called Amniotic Membrane Transplantation, and then made all the arrangements to have a consultation with him within 2 and ½ weeks.. I have Sjogrens Syndrome and for 8 years I have been trying to get help and relief from the acute debilitating pain, burning irritation and discomfort of always feeling as though I have a large piece of foreign matter in my eyes, as well as though grit has been thrown into my eyes and they were being rubbed with sandpaper.. Sometimes I felt as though my left eye, particularly, was being pierced with a sharp instrument and it was being twisted to cause the maximum pain. Repeatedly, I would say to the doctors here: " I cant believe that my eyes feel like this-yet you cant see anything".. I had tried every type of drops, ointment, lubricants, antibiotic drops for eyes, steroid drops and autologous ...
Explaination: Explanation: Placenta and chorion compose the fetal-maternal junction. The former is a trophoblast derivative and the latter is a derivative of both trophoblast and extraembryonic mesoderm. The other extraembryonic tissues such as the amnion, the yolk sac and the allantois are derived from the inner cell mass. Among them, the yolk sac and the allantois are endodermal derivatives whereas the amnion is an ectodermal derivative ...
Free, official info about 2015 ICD-9-CM diagnosis code 762.8. Includes coding notes, detailed descriptions, index cross-references and ICD-10-CM conversion info.
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NEW WORKS FROM NORA RENAUDS ADAL III PROJECT- a continuation of her previous two ADAL collections. The series consists of jewellery, drawings, and design objects, including a special edition candle holder produced in collaboration with SOME/THINGS AGENCY ...
This stock medical exhibit features normal fetal membrane anatomy during pregnancy. It reveals a sagittal cut-away view of a full term pregnant abdomen with fetus shown in-utero. A blue colored membranous capsule is shown surrounding the fetus. Additionally, a detailed section through the membrane identifies and labels the amnion, chorion, uterus and decidua.
This stock medical exhibit features normal fetal membrane anatomy during pregnancy. It reveals a sagittal cut-away view of a full term pregnant abdomen with fetus shown in-utero. A blue colored membranous capsule is shown surrounding the fetus. Additionally, a detailed section through the membrane identifies and labels the amnion, chorion, uterus and decidua.
My doc excised two pterygium from the same eye (one located toward the inner part nasal and one located toward the outer part tempor
The mechanisms responsible for the onset of labor in women are not completely understood. Growing evidence suggests that bacterial products such as endotoxin could stimulate monocytes or macrophages to produce inflammatory cytokines such as IL-1, TNF, IL-6 and IL-8, which in turn stimulate prostaglandin biosynthesis by amnion, chorion and decidua and result in the onset of labor. Amniotic fluid IL-6 could be a sensitive indicator for prospective diagnosis of acute histologic chorioamnionitis, so amniotic fluid IL-6 is considered as an important cytokine. IL-13 and TIMP-3, recently described, have been known to relate to IL-6. IL-13, a novel T-helper cell lymphokin, has been shown to upregulate IL-6 production in keratinocytes, endothelial cells and mesothelial cells, whereas it downregulates IL-6 production in human monocytes. Tissue inhibitor of metalloproteinase-3(TIMP-3), new type distinct from TIMP-1 and TIMP-2, is known to be stimulated by IL-6. In this study , we examined C3H pregnant mice ...
FIG 11.1 Incision line opening. (A) Incision line opening occurring after implant placement. (B) Post allograft incision line opening. (C) Membrane graft dehiscence. (D) Block graft with fixation screw exposure. The prevalence of ILO has been shown in studies to range from 4.6% to 40% around submerged implants.1,2 In a study by Mendoza, 37% had no…
When few membranes are produced by mother, it should take more care for their survival. If the number are more, care will be less. In amniotes when the developing embryo is enveloped, by extra embryonic membranes, which will give scope, for developing embryo, the extra em-bryonic membranes are chorion, amnion, yolk sac, allantois. ...
A human embryo of the fourth week. I, the chorion; 3, part of the amnion; 4, umbilical vesicle with its long pedicle passing into the abdomen; 7, the heart; 8, the liver; 9, the visceral arch destined to form the lower jaw, beneath which are two other visceral arches separated by the branchial clefts; 10, rudiment of the upper extremity, 11, that of the lower extremity; 12, the umbilical cord; 15, the eye; 16, the ear; 17, cerebral hemispheres; 18, optic lobes, corpora quadrigemina.. ...
Anatomical structure in a pouch. Gestational sac: membranes enveloping the foetus or embryo. Composed by the fusion of chorion and amnion.
Anatomical structure in a pouch. Gestational sac: membranes enveloping the foetus or embryo. Composed by the fusion of chorion and amnion.
This line was originally thought to be derived from normal amnion, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.
Functional Network Analysis of the Transcriptomes of Mesenchymal Stem Cells Derived from Amniotic Fluid, Amniotic Membrane, Cord Blood, and Bone Marrow (pages 2511-2523). Ming-Song Tsai, Shiaw-Min Hwang, Kuang-Den Chen, Yun-Shien Lee, Li-Wen Hsu, Yu-Jen Chang, Chao-Nin Wang, Hsiu-Huei Peng, Yao-Lung Chang, An-Shine Chao, Shuenn-Dyh Chang, Kuan-Der Lee, Tzu-Hao Wang, Hsin-Shih Wang and Yung-Kuei Soong. Version of Record online: 7 JUN 2007 , DOI: 10.1634/stemcells.2007-0023. ...
Therefore, ex vivo expansion of BMSCs is required to obtain a sufficient number of transplantable cells. Since BMSCs require several kinds of supportive factors for their growth, it is standard practice to use fetal bovine serum (FBS), while autologous human serum (HS) and pooled allogeneic HS have also been used. It has been suggested that FBS may not be favorable for clinical applications due to the possible risk of contamination (prions, viruses, zoonosis) or immunological reactions against xenogeneic serum antigens (Agata et al., 2009). ...
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PURPOSE. A previous report has described an ocular surface reconstruction method involving the use of cultivated corneal epithelium derived from limbal explants. In the current study, a new culture system was developed involving the in vitro propagation on amniotic membrane (AM) of epithelial cells from enzymatically dissociated limbal epithelium. The purpose of this new method is to produce a cultivated epithelial cell layer that contains stem cells and that is superior to explanted cultivated epithelium. The new cell-suspension technique was compared with the existing explant method. METHODS. Limbal epithelial cells were dissociated from donor eyes by dispase and seeded on the denuded AM. Small pieces of limbal epithelium were also cultured on denuded AM as explant cultures. The cultivated epithelium was examined by electron microscopy and immunohistochemistry for cornea-specific keratins (K3 and K12). RESULTS. Both cell-suspension and explant culture methods produced a healthy epithelial cell ...
BACKGROUNDS: Little information is currently available on the long-term safety of eyes following limbal tissue donation. We have therefore evaluated long-term refractive changes, corneal surface stability, and visual outcome following limbal tissue donation of 180 degrees with permanent amniotic membrane transplantation (AMT). METHODS: Four healthy eyes of four patients underwent limbal keratectomy involving half of the limbal circumference (half limbal) for the purpose of limbal donation with permanent AMT. The best corrected visual acuity (BCVA), astigmatic changes by manifest refraction, and corneal surface changes as assessed by slit-lamp examination and by impression cytology were evaluated. RESULTS: The mean follow-up period was 20.8 months (range: 19-24 months). BCVA remained unchanged in three eyes (75%), but eye one showed a BCVA decrease of two lines due to newly developed filamentary keratitis caused by Sjogrens syndrome. The average astigmatism change was an increase of 0.31 ...
TY - JOUR. T1 - Enrichment in c-Kit improved differentiation potential of amniotic membrane progenitor/stem cells. AU - Resca, E.. AU - Zavatti, M.. AU - Maraldi, T.. AU - Bertoni, L.. AU - Beretti, F.. AU - Guida, M.. AU - La Sala, G. B.. AU - Guillot, P. V.. AU - David, A. L.. AU - Sebire, N. J.. AU - De Pol, A.. AU - De Coppi, P.. PY - 2015/1/1. Y1 - 2015/1/1. N2 - Introduction Human term placenta has attracted increasing attention as an alternative source of stem cells for regenerative medicine since it is accessible without ethical objections. The amniotic membrane (AM) contains at least two stem cell types from different embryological origins: ectodermal amniotic epithelial stem cells, and mesodermal mesenchymal stromal cells. Among the second group we studied the characteristics of amniotic mesenchymal cells (AMC) versus the ones enriched for the commonly used surface marker c-Kit (amniotic progenitor/stem cells-ASC), a stem cell factor receptor with crucial functions in a variety of ...
The hypothesis of an intrinsic defect of germ plasma put forward by Streeter to explain the abnormalities observed in amniotic bands sequence (ABS) was not supported by Torpin, who suggested that the bands derived from early amnion rupture, with formation of mesodermal strings and naked chorion, which then constricted or adhered to fetal parts. Recently, several authors have recorded and discussed possible mechanisms for non-band related malformations in patients with otherwise typical limb constrictions and amputations. The mouse mutant disorganisation (Ds) is a semidominant with 72% of heterozygotes manifesting abnormalities, which include cranioschisis, limb duplications and deficiencies, gastro/thoracoschisis, and papillae protruding from other parts of the body. We report similar abnormalities including papillae in five fetuses and one newborn with ABS and, based on these observations and published reports, we suggest that a human homologue for Ds may be the cause of at least some ...
Purpose: To describe a standardized, xenogenic-free protocol for the manufacture of limbal epithelial stem cell grafts and a no touch surgical technique for its standardized transplantation. Setting: Antwerp University Hospital, Antwerp, Belgium. Methods: The limbo-amnion composite graft is generated by cultivating limbal epithelial stem cells on a standardized (thermolysin treated and spongy layer removed) amniotic membrane, stretched within an interlockable amnion ring. The cells are cultured in CnT-20 medium with the addition of 1% human AB serum for a period of 2 weeks. Fibrin glue is applied to the surgically prepared recipients cornea and in one fluid motion, the composite graft within the amnion ring construct is transferred from culture and positioned onto the graft bed. The required size is cut out at the level of the limbus by means of a trephine and/or microsurgical scissors. Results: The lightweight, plastic interlockable ring offered stability to the graft during culture, ...
Abstract Objective: A population of stem cells (SCs) is responsible for maintenance and repair of the ocular surface. These stem cells can become deficient as a result of various diseases affecting the ocular surface. Depending on the extent of damage, the clinical features can vary from mild discomfort to substantial pain and/or blindness. The growing field of tissue engineering and advances in stem cell research offer promising new alternatives for these disorders. This student thesis will provide an overview of the human ocular surface (cornea, limbus, and conjunctiva), ocular surface stem cell deficiency, and treatment possibilities. Methods: This student thesis is based on a number of review articles and original articles on PubMed using the search words limbal, limbal stem cell deficiency, conjunctival and/or ocular surface reconstruction. Only papers written in English were considered. Findings: There are different possible methods for treating ocular surface stem cell deficiency by ...
Limbal epithelial stem cells (LESCs) are tissue-specific stem cells responsible for renewing the corneal epithelium. Acute trauma or chronic disease affecting LESCs may disrupt corneal epithelial renewal, causing vision threatening and painful ocular surface disorders, collectively referred to as LESC deficiency (LESCD). These disorders cannot be treated with traditional corneal transplantation and therefore alternative cell sources for successful cell-based therapy are needed. LESCs derived from human pluripotent stem cells (hPSCs) are a prospective source for ocular surface reconstruction, yet critical evaluation of these cells is crucial before considering clinical applications. In order to quantitatively evaluate hPSC-derived LESCs, we compared protein expression in native human corneal cells to that in hPSC-derived LESCs using isobaric tag for relative and absolute quantitation (iTRAQ) technology. We identified 860 unique proteins present in all samples, including proteins involved in cell ...
Objective. To evaluate the maternal inflammatory response to microbial invasion of the amniotic cavity (MIAC) in women with preterm labor and preterm prelabor rupture of membranes using selected proteins in the maternal serum. Design. A prospective cohort study. Setting. Labor ward from Salgrenska University Hospital. The evaluation of the maternal inflammatory response in the presence of MIAC in preterm labor and preterm prelabor rupture of membranes. Population. One hundred and sixteen women with preterm labor and 73 women with preterm prelabor rupture of membranes between the gestational ages of 22+0 and 33+6 weeks. Methods. Twenty-seven maternal serum proteins were assayed by a multiple immunoassay. Main outcome measures. The maternal serum inflammatory response was evaluated according to the presence of MIAC. Data were stratified by gestational age. Results. There were few differences in the maternal serum protein levels when MIAC was present in both preterm labor and preterm prelabor ...
Before clinical trials can be performed, a safe and validated protocol for limbal stem cell culturing needs to be established. Therefore, an experimental study was started in March 2014 in Ljubljana Eye Hospital, after the National Medical Ethics Committee approved (123/02/14, date: 25. 3. 2014) all the procedures and protocols. The research followed the tenets of the Declaration of Helsinki. The purpose of the research was to validate our culturing protocol using a medium supplemented with only human serum and AM as a substrate carrier for the cultivation of limbal epithelial cells, using the limbal explant technique (21). Cryopreserved human AM were provided by the Blood Transfusion Centre of Ljubljana, Slovenia, and were preserved according to the method described by Koizumi et al (22). After the central corneal buttons were used for corneal transplantation purposes, we used the preserved cadaveric human corneo-scleral rims for limbal explant preparation. As shown in Figure 2A, each limbal ...
Faculty directly responsible for education and training in this fellowship include Dr. Davis, Dr. Rhea Siatkowski, and Dr. David Jackson, Drs. Davis and Siatkowskis practices in cornea and external diseases are similar, and include patients with infectious, inflammatory, neoplastic, traumatic, and degenerative disorders of the cornea, ocular surface, and anterior segment. Recent fellows have performed over 40 penetrating and deep anterior lamellar keratoplasties, 50 DSAEK, 100+ phaco/IOL, and 15-30 refractive surgeries as primary surgeon. Additional expertise will be developed in secondary lens implants, iris reconstruction, keratoprostheses, ocular surface reconstruction, limbal stem cell transplantation, ocular surface neoplasm excision, and in oculoplastic procedures relevant to the treatment of corneal diseases.. In addition to this exposure to a tremendous breadth and depth of cornea and external disease pathology, the fellow also will participate in the evaluation and management of ...
Current therapies for preterm labour (PTL) focus on arresting myometrial contractions but are largely ineffective, thus alternative therapeutic targets need to be identified. Leukocytes infiltrate the uterus around the time of labour, and are in particularly abundant in decidua (maternal-fetal interface). Moreover, decidual inflammation precedes labour in rat pregnancies and thus may contribute to initiation of labour. We hypothesized that chemokines mediate decidual leukocyte trafficking during preterm labour (PTL) and term labour (TL), thus representing potential targets for preventing PTL. Women were recruited into 4 groups: TL, term not in labour (TNL), idiopathic PTL and PTL with infection (PTLI). Choriodecidual RNA was subjected to a pathway-specific PCR array for chemokines. Differential expression of 12 candidate chemokines was validated by real time RT-PCR and Bioplex assay, with immunohistochemistry to confirm cellular origin. 25 chemokines were upregulated in choriodecidua from TL compared to

Amnions synonyms, amnions antonyms - FreeThesaurus.comAmnions synonyms, amnions antonyms - FreeThesaurus.com

Antonyms for amnions. 2 synonyms for amnion: amnios, amniotic sac. What are synonyms for amnions? ... amnion. (redirected from amnions). Also found in: Dictionary, Medical, Encyclopedia. #vtZoom,.vt-link{cursor:pointer} .vt- ... Synonyms for amnion. thin innermost membranous sac enclosing the developing embryo of higher vertebrates (reptiles, birds and ... The presence of 2 chorions and amnions at birth confirm that the twins are dichorionic/diamniotic identical twins arising from ...
more infohttp://www.freethesaurus.com/amnions

Amniotic Band Syndrome - NORD (National Organization for Rare Disorders)Amniotic Band Syndrome - NORD (National Organization for Rare Disorders)

According to this theory, amniotic band syndrome occurs when the inner layer (amnion) of the amniotic sac ruptures or tears, ... demonstrated this theory of vascular disruption with an intact amnion in animal models. ... amnion) of the amniotic sac. The amniotic sac is the thin membrane that completely surrounds an embryo or developing fetus ( ... the outer layer is called the chorion and the inner layer is called the amnion. ...
more infohttps://rarediseases.org/rare-diseases/amniotic-band-syndrome/

New Research Shows Versatility of Amniotic Fluid Stem CellsNew Research Shows Versatility of Amniotic Fluid Stem Cells

The fact that the amnion cells are less likely to form tumors may be one advantage that they have over embryonic stem cells in ... "Amnion cells are on the way to become an important source for both basic science and regenerative medicine." ... "This finding suggests that the amnion cells have greater potential than we originally thought and may be able to form many cell ... "Performing many independent experiments using different approaches, we demonstrate in the report that human amnion stem cells ...
more infohttp://www.innovations-report.com/html/reports/life-sciences/research-shows-versatility-amniotic-fluid-stem-cells-144190.html

Human amniotic fluid stem cells have a potential to recover ovarian function in mice with chemotherapy-induced sterility | BMC...Human amniotic fluid stem cells have a potential to recover ovarian function in mice with chemotherapy-induced sterility | BMC...

B) Human amnion epithelial cells (hAEC) grown in culture for comparison. (C) Comparison of relative gene expression levels for ... the cell morphology mimicked that of mesenchymal cells rather than the typical epithelial morphology of human amnion epithelial ...
more infohttps://bmcdevbiol.biomedcentral.com/articles/10.1186/1471-213X-13-34

Biocompatible collagen scaffolds from a human amniotic membrane: ...: Ingenta ConnectBiocompatible collagen scaffolds from a human amniotic membrane: ...: Ingenta Connect

A reconstituted collagen membrane from human amnion has been investigated as a source of collagen matrix, which could be used ...
more infohttp://www.ingentaconnect.com/content/tandf/bsp/2003/00000014/00000007/art00007

Amniotic Band? - BabyandBumpAmniotic Band? - BabyandBump

These are called the inner amnion and the outer chorion, and they are separate in early pregnancy. It can cause serious ...
more infohttp://babyandbump.momtastic.com/gestational-complications/2408749-amniotic-band.html

Amniotic Band SyndromeAmniotic Band Syndrome

ABS is typically caused when there is damage to the amnion, a part of the placenta. Placenta damages has been linked to ...
more infohttp://www.zofranlawsuitguide.com/zofran-birth-defects/amniotic-band-syndrome/

Amnion | anatomy | Britannica.comAmnion | anatomy | Britannica.com

Amnion,, in reptiles, birds, and mammals, a membrane forming a fluid-filled cavity (the amniotic sac) that encloses the embryo ... More About Amnion. 5 references found in Britannica articles. animal. *formation and function in embryo* In animal development ... Amnion, in reptiles, birds, and mammals, a membrane forming a fluid-filled cavity (the amniotic sac) that encloses the embryo. ... In development, the amnion arises by a folding of a mass of extra-embryonic tissue called the somatopleure. Lined with ectoderm ...
more infohttps://www.britannica.com/science/amnion

Amnion and Germ LayersAmnion and Germ Layers

Cells from the epiblast form a membrane called the amnion, within which the embryo and later the fetus develop until birth. ... Amnion and Germ Layers. Select Subtitle Language. 88 languages available. Afrikaans. Albanian (Tosk) [Shqip]. Arabic [عربي]. ...
more infohttps://www.ehd.org/movies/9/Amnion-and-Germ-Layers

amnion Archives » New York Eye Cancer Centeramnion Archives » New York Eye Cancer Center

Thus, amnion can provide a foundational platform for new cells to grow and flourish. In this case series, super thick amniotic ...
more infohttps://eyecancer.com/tag/amnion/

Amnion - WikipediaAmnion - Wikipedia

... the inner forming the amnion, the outer the false amnion or serosa. The space between the amnion and the serosa constitutes the ... For the alien race in Stephen R. Donaldsons The Gap Cycle, see Amnion (Gap Cycle). The amnion is a membrane that closely ... The amnion, along with the chorion, the yolk sac and the allantois form a protective sac around the embryo. In birds, reptiles ... The amnion stems from the extra-embryonic somatic mesoderm on the outer side and the extra-embryonic ectoderm on the inner side ...
more infohttps://en.wikipedia.org/wiki/Amnion

The Biomechanics of Amnion Rupture: An X-Ray Diffraction StudyThe Biomechanics of Amnion Rupture: An X-Ray Diffraction Study

... of which are attributed to the pre-term premature rupture of amnion. Rupture of amnion is thought to be associated with a ... These data provide an accurate insight into the biomechanical process of amnion rupture and highlight X-ray diffraction as a ... However, there is very little information concerning the detailed organisation of fibrillar collagen in amnion and how this ... Amnion Is the Subject Area "Amnion" applicable to this article? Yes. No. ...
more infohttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0001147

Placenta, Cord, Amnion, Chorion Childbirth ModelPlacenta, Cord, Amnion, Chorion Childbirth Model

... amnion, and chorion is perfect for childbirth education courses. Visit Cascade HealthCare Products secure Website to view our ... Amnion, Chorion Model - $89 A great teaching tool, this model of the placenta, cord, ... A great teaching tool, this model of the placenta, cord, amnion, and chorion is perfect for childbirth education courses. Fetal ...
more infohttps://www.1cascade.com/placenta-cord-amnion-chorion-model-training-aid

Amnion nodosum - WikipediaAmnion nodosum - Wikipedia

Amnion nodosum are nodules on the fetal surface of the amnion, and is frequently present in oligohydramnios. The nodules are ... Amnion nodosum is granules on amnion whereas whitish nodules on the cord suggest a candidial infection. Adeniran AJ, Stanek J ( ... Amnion nodosum and oligohydromnios are associated with pulmonary hypoplasia and renal agenesis. ... 2007). "Amnion nodosum revisited: clinicopathologic and placental correlations". Arch Pathol Lab Med. 131 (12): 1829-33. doi: ...
more infohttps://en.wikipedia.org/wiki/Amnion_nodosum

Epigenetic Regulation of Cytokine Production in Human Amnion and Villous PlacentaEpigenetic Regulation of Cytokine Production in Human Amnion and Villous Placenta

... Murray D. Mitchell,1 Anna P. Ponnampalam,2 ... Murray D. Mitchell, Anna P. Ponnampalam, and Gregory E. Rice, "Epigenetic Regulation of Cytokine Production in Human Amnion and ...
more infohttps://www.hindawi.com/journals/mi/2012/159709/cta/

Amnion nodosum | definition of amnion nodosum by Medical dictionaryAmnion nodosum | definition of amnion nodosum by Medical dictionary

... amnion nodosum explanation free. What is amnion nodosum? Meaning of amnion nodosum medical term. What does amnion nodosum mean? ... Looking for online definition of amnion nodosum in the Medical Dictionary? ... Related to amnion nodosum: breech presentation, Prune belly syndrome. amnion. [am´ne-on] the innermost fetal membrane, which ... amnion nodo´sum a nodular condition of the fetal surface of the amnion, usually appearing near the insertion of the cord; it ...
more infohttp://medical-dictionary.thefreedictionary.com/amnion+nodosum

amnionamnion

... , membrane around the fetus] (ARTHROPODA) The inner cellular, membranous embryonic covering of various insects and other ... amnion - [am′nē ən, am′nēän΄] n. pl. amnions or amnia [am′nēə] [Gr, dim. of amnos, lamb < IE * agwhnos > YEAN, L agnus] 1 ... amnion - (n.) 1660s, Modern Latin, from Gk. amnion membrane around a fetus, said to be originally bowl in which the blood of ... amnion - amnion. См. амнион. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., ...
more infohttp://en.academic.ru/dic.nsf/en_invertebrate_zoology/679/amnion

Amnion - iPodiatryAmnion - iPodiatry

Amnion. Amnion and amniotic tissue has been studied for more than 100 years in the treatment of acute and chronic wounds. ... Amnion and amniotic tissue has been studied for more than 100 years in the treatment of acute and chronic wounds. Recent ... Recent studies have focused on the use of amnion tissue in the management of full-thickness diabetic wounds, particularly of ... With new harvesting, processing, and distribution technologies, amnion is increasingly available in treating these wounds. ...
more infohttp://ipodiatry.org/amnion/29752/

Amnion | Radiology Reference Article | Radiopaedia.orgAmnion | Radiology Reference Article | Radiopaedia.org

Amnion refers to a membranous structure which covers and protects the embryo. It forms inside the chorion. The amnion usually ... Radiographic features Ultrasound The amnion can be visu... ... "Amnion" derives from the Greek word "άμνίον", a bowl in which ... Amnion refers to a membranous structure which covers and protects the embryo. It forms inside the chorion. The amnion usually ... The amnion can be visualised in most pregnancies before the 12th week of gestation and appears as a thin membrane separating ...
more infohttps://radiopaedia.org/articles/amnion

Telomere Fragment Induced Amnion Cell Senescence: A Contributor to Parturition?Telomere Fragment Induced Amnion Cell Senescence: A Contributor to Parturition?

In vitro treatment of primary human amnion epithelial cells with 40 μM T-oligos ([TTAGGG]2) that mimic telomere fragments, ... In vitro and in situ telomere fragments increased human and murine amnion p38MAPK, senescence and inflammatory cytokines. We ... could induce human and murine amnion cell senescence. In a cross-sectional clinical study, AF telomere fragment concentrations ... Amnion Is the Subject Area "Amnion" applicable to this article? Yes. No. ...
more infohttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0137188

Amnion Epithelial Cells: Cell Therapy Going Green! | Science Translational MedicineAmnion Epithelial Cells: Cell Therapy Going Green! | Science Translational Medicine

In this study, human amnion epithelial cells (hAECs) were derived from amnion obtained from placentas that were discarded after ... Amnion Epithelial Cells: Cell Therapy Going Green! Message Subject. (Your Name) has forwarded a page to you from Science ... Recycling human amnion allows the production of stemlike cells that differentiate into airway epithelium and soothe injured ... Recycling human amnion allows the production of stemlike cells that differentiate into airway epithelium and soothe injured ...
more infohttp://stm.sciencemag.org/content/2/48/48ec138

Worlds 1st Trial of Amnion Stem Cells for Premature BabiesWorld's 1st Trial of Amnion Stem Cells for Premature Babies

The amnion and chorion ("amnion" hereafter) form a membrane that surrounds the baby during pregnancy and plays an important ... Amnion Epithelial Cells (AECs) are a stem-like cell that can be isolated in large numbers (150 to 200 million per gram) from ... The fetal membranes - amnion and chorion - also contain stem cells and stem cell-like cells that can be isolated and banked for ... The amnion dressings promote cell growth, modulate inflammation and recruit endogenous (local) stem cells to facilitate wound ...
more infohttps://parentsguidecordblood.org/ru/node/17893

Disorganisation: a model for early amnion rupture? | Journal of Medical GeneticsDisorganisation: a model for 'early amnion rupture'? | Journal of Medical Genetics

... who suggested that the bands derived from early amnion rupture, with formation of mesodermal strings and naked chorion, which ...
more infohttp://jmg.bmj.com/content/26/7/421

BMP-dependent serosa and amnion specification in the scuttle fly Megaselia abdita | DevelopmentBMP-dependent serosa and amnion specification in the scuttle fly Megaselia abdita | Development

Tc-dpp RNAi suppresses the expression of Tc-pnr, an early amnion marker, which indicates that Tc-dpp is required for amnion ... However, although amnion specification might begin at the blastoderm stage, the distinction of lateral amnion cells from ... We suspect that these cells give rise to the amnion and propose that amnion specification occurs, at least laterally, after the ... Role of specific BMP signaling components in serosa and amnion specification. To test whether serosa and amnion specification ...
more infohttps://dev.biologists.org/content/139/18/3373?ijkey=60a9a71cae0096e38b33487efbf92e76c8ed0b5d&keytype2=tf_ipsecsha

Human Amnion Epithelial Cells Review Articles|OMICS International|Journal Of Pregnancy And Child HealthHuman Amnion Epithelial Cells Review Articles|OMICS International|Journal Of Pregnancy And Child Health

The human amnion epithelial cells (hAECs) resultant from term placenta are anatomically and histologically focused fetal ... Human Amnion Epithelial Cells Review Articles. The human amnion epithelial cells (hAECs) resultant from term placenta are ...
more infohttps://www.omicsonline.org/pregnancy-child-health/human-amnion-epithelial-cells-review-articles.php
  • On cross sections of rolled amnion membranes, regenerating axons of cultured neurons were guided along pathways of basement membrane that were immunoreactive with an antibody to laminin. (sciencemag.org)
  • In addition, when rolled amnion membranes were implanted into a lesion cavity between the rat septum and hippocampus, cholinergic neurons extended axons through the longitudinally oriented implant into the hippocampus. (sciencemag.org)
  • After the fusion of the edges of the amniotic fold, the two layers of the fold become completely separated, the inner forming the amnion, the outer the false amnion or serosa. (wikipedia.org)
  • The space between the amnion and the serosa constitutes the extra-embryonic celom, and for a time communicates with the embryonic celom. (wikipedia.org)
  • To better understand potential evolutionary links between BMP signaling and extra-embryonic tissue specification, we examined the activity profile and function of BMP signaling in serosa and amnion patterning of the scuttle fly Megaselia abdita (Phoridae) and compared the BMP activity profiles between M. abdita and D. melanogaster . (biologists.org)
  • This transition correlated with the first signs of amnion differentiation laterally adjacent to the serosa anlage. (biologists.org)
  • Marker-assisted analysis of six BMP signaling components ( dpp, gbb, scw, tkv, sax, sog ) by RNA interference revealed that both serosa and amnion specification of M. abdita are dependent on BMP activity. (biologists.org)
  • The mechanism by which rTIMP inhibits amniotic membrane invasion was compared to the mechanism by which the fibronectin receptor binding peptide RGDS and the laminin receptor binding peptide YIGSR inhibit amnion invasion. (aacrjournals.org)
  • The amnion is a protective sac that surrounds a baby in the mother's womb. (study.com)
  • Telomere Fragment Induced Amnion Cell Senescence: A Contributor to Parturition? (plos.org)
  • Eventually, the baby will float inside the balloon-like amnion, which protects it and allows it to grow and develop safely. (study.com)
  • With new harvesting, processing, and distribution technologies, amnion is increasingly available in treating these wounds. (ipodiatry.org)
  • Is the Subject Area "Amnion" applicable to this article? (plos.org)
  • In contrast rTIMP did not inhibit the cell adhesion step in amnion invasion, but actually increased the number of tumor cells that were tightly bound to the amnion. (aacrjournals.org)
  • Amnion-Derived Multipotent Progenitor Cells Inhibit Blood Monocyte Differentiation Into Mature Dendritic Cells. (biolifesolutions.com)
  • Banas R, Miller C, Guzik L, Zeevi A. Amnion-Derived Multipotent Progenitor Cells Inhibit Blood Monocyte Differentiation Into Mature Dendritic Cells. (biolifesolutions.com)
  • Diagram of a transverse section, showing the mode of formation of the amnion in the chick. (wikipedia.org)
  • This kind of amnion is known as pleuroamnion (formed by folding), as opposed to schyzoamnion (formed by delamination). (wikipedia.org)
  • To understand the way the amnion develops, let's go back to space for a moment. (study.com)