Ammonium Sulfate: Sulfuric acid diammonium salt. It is used in CHEMICAL FRACTIONATION of proteins.Quaternary Ammonium Compounds: Derivatives of ammonium compounds, NH4+ Y-, in which all four of the hydrogens bonded to nitrogen have been replaced with hydrocarbyl groups. These are distinguished from IMINES which are RN=CR2.Heparitin Sulfate: A heteropolysaccharide that is similar in structure to HEPARIN. It accumulates in individuals with MUCOPOLYSACCHARIDOSIS.Molecular Weight: The sum of the weight of all the atoms in a molecule.Ammonium Chloride: An acidifying agent that has expectorant and diuretic effects. Also used in etching and batteries and as a flux in electroplating.Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Chemical Precipitation: The formation of a solid in a solution as a result of a chemical reaction or the aggregation of soluble substances into complexes large enough to fall out of solution.Sulfates: Inorganic salts of sulfuric acid.Fractional Precipitation: A method which uses specific precipitation reactions to separate or collect substances from a solution.Chromatography, Gel: Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.Ammonium Compounds: Inorganic compounds that include a positively charged tetrahedral nitrogen (ammonium ion) as part of their structure. This class of compounds includes a broad variety of simple ammonium salts and derivatives.Chromatography, Ion Exchange: Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins.Dermatan Sulfate: A naturally occurring glycosaminoglycan found mostly in the skin and in connective tissue. It differs from CHONDROITIN SULFATE A (see CHONDROITIN SULFATES) by containing IDURONIC ACID in place of glucuronic acid, its epimer, at carbon atom 5. (from Merck, 12th ed)Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Chromatography: Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.Kinetics: The rate dynamics in chemical or physical systems.Heparan Sulfate Proteoglycans: Ubiquitous macromolecules associated with the cell surface and extracellular matrix of a wide range of cells of vertebrate and invertebrate tissues. They are essential cofactors in cell-matrix adhesion processes, in cell-cell recognition systems, and in receptor-growth factor interactions. (From Cancer Metastasis Rev 1996; 15(2): 177-86; Hepatology 1996; 24(3): 524-32)Crystallization: The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Keratan Sulfate: A sulfated mucopolysaccharide initially isolated from bovine cornea. At least two types are known. Type I, found mostly in the cornea, contains D-galactose and D-glucosamine-6-O-sulfate as the repeating unit; type II, found in skeletal tissues, contains D-galactose and D-galactosamine-6-O-sulfate as the repeating unit.Chondroitin Sulfate Proteoglycans: Proteoglycans consisting of proteins linked to one or more CHONDROITIN SULFATE-containing oligosaccharide chains.Amino Acids: Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.Chromatography, DEAE-Cellulose: A type of ion exchange chromatography using diethylaminoethyl cellulose (DEAE-CELLULOSE) as a positively charged resin. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Isoelectric Point: The pH in solutions of proteins and related compounds at which the dipolar ions are at a maximum.Dextran Sulfate: Long-chain polymer of glucose containing 17-20% sulfur. It has been used as an anticoagulant and also has been shown to inhibit the binding of HIV-1 to CD4-POSITIVE T-LYMPHOCYTES. It is commonly used as both an experimental and clinical laboratory reagent and has been investigated for use as an antiviral agent, in the treatment of hypolipidemia, and for the prevention of free radical damage, among other applications.Chondroitin Sulfates: Derivatives of chondroitin which have a sulfate moiety esterified to the galactosamine moiety of chondroitin. Chondroitin sulfate A, or chondroitin 4-sulfate, and chondroitin sulfate C, or chondroitin 6-sulfate, have the sulfate esterified in the 4- and 6-positions, respectively. Chondroitin sulfate B (beta heparin; DERMATAN SULFATE) is a misnomer and this compound is not a true chondroitin sulfate.Ammonia: A colorless alkaline gas. It is formed in the body during decomposition of organic materials during a large number of metabolically important reactions. Note that the aqueous form of ammonia is referred to as AMMONIUM HYDROXIDE.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Sodium Dodecyl Sulfate: An anionic surfactant, usually a mixture of sodium alkyl sulfates, mainly the lauryl; lowers surface tension of aqueous solutions; used as fat emulsifier, wetting agent, detergent in cosmetics, pharmaceuticals and toothpastes; also as research tool in protein biochemistry.Temperature: The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.Chromatography, Affinity: A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Ammonium Hydroxide: The hydroxy salt of ammonium ion. It is formed when AMMONIA reacts with water molecules in solution.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Chemical Fractionation: Separation of a mixture in successive stages, each stage removing from the mixture some proportion of one of the substances, for example by differential solubility in water-solvent mixtures. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Isoelectric Focusing: Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point.Immunodiffusion: Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.Nitrogen: An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.Enzyme Stability: The extent to which an enzyme retains its structural conformation or its activity when subjected to storage, isolation, and purification or various other physical or chemical manipulations, including proteolytic enzymes and heat.X-Ray Diffraction: The scattering of x-rays by matter, especially crystals, with accompanying variation in intensity due to interference effects. Analysis of the crystal structure of materials is performed by passing x-rays through them and registering the diffraction image of the rays (CRYSTALLOGRAPHY, X-RAY). (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Glycosaminoglycans: Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine or N-acetylgalactosamine.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Electrophoresis, Disc: Electrophoresis in which discontinuities in both the voltage and pH gradients are introduced by using buffers of different composition and pH in the different parts of the gel column. The term 'disc' was originally used as an abbreviation for 'discontinuous' referring to the buffers employed, and does not have anything to do with the shape of the separated zones.Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Magnesium Sulfate: A small colorless crystal used as an anticonvulsant, a cathartic, and an electrolyte replenisher in the treatment of pre-eclampsia and eclampsia. It causes direct inhibition of action potentials in myometrial muscle cells. Excitation and contraction are uncoupled, which decreases the frequency and force of contractions. (From AMA Drug Evaluations Annual, 1992, p1083)Hot Temperature: Presence of warmth or heat or a temperature notably higher than an accustomed norm.Chromatography, High Pressure Liquid: Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Hydroxyapatites: A group of compounds with the general formula M10(PO4)6(OH)2, where M is barium, strontium, or calcium. The compounds are the principal mineral in phosphorite deposits, biological tissue, human bones, and teeth. They are also used as an anticaking agent and polymer catalysts. (Grant & Hackh's Chemical Dictionary, 5th ed)Sulfotransferases: Enzymes which transfer sulfate groups to various acceptor molecules. They are involved in posttranslational sulfation of proteins and sulfate conjugation of exogenous chemicals and bile acids. EC 2.8.2.Glycoside HydrolasesDisaccharides: Oligosaccharides containing two monosaccharide units linked by a glycosidic bond.Copper Sulfate: A sulfate salt of copper. It is a potent emetic and is used as an antidote for poisoning by phosphorus. It also can be used to prevent the growth of algae.Proteoglycans: Glycoproteins which have a very high polysaccharide content.Bacterial Proteins: Proteins found in any species of bacterium.Chemistry: A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.Drug Stability: The chemical and physical integrity of a pharmaceutical product.Macromolecular Substances: Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.Chemical Phenomena: The composition, conformation, and properties of atoms and molecules, and their reaction and interaction processes.Zinc Sulfate: A compound given in the treatment of conditions associated with zinc deficiency such as acrodermatitis enteropathica. Externally, zinc sulfate is used as an astringent in lotions and eye drops. (Reynolds JEF(Ed): Martindale: The Extra Pharmacopoeia (electronic version). Micromedex, Inc, Englewood, CO, 1995)Bacteriocins: Substances elaborated by specific strains of bacteria that are lethal against other strains of the same or related species. They are protein or lipopolysaccharide-protein complexes used in taxonomy studies of bacteria.Rabbits: The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.Chondroitin: A mucopolysaccharide constituent of chondrin. (Grant & Hackh's Chemical Dictionary, 5th ed)Carbohydrates: The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.Crystallography, X-Ray: The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Solubility: The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Peptide Hydrolases: Hydrolases that specifically cleave the peptide bonds found in PROTEINS and PEPTIDES. Examples of sub-subclasses for this group include EXOPEPTIDASES and ENDOPEPTIDASES.Dehydroepiandrosterone Sulfate: The circulating form of a major C19 steroid produced primarily by the ADRENAL CORTEX. DHEA sulfate serves as a precursor for TESTOSTERONE; ANDROSTENEDIONE; ESTRADIOL; and ESTRONE.Hydrolysis: The process of cleaving a chemical compound by the addition of a molecule of water.Pseudomonas: A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants.Spectrophotometry: The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.Bacillus: A genus of BACILLACEAE that are spore-forming, rod-shaped cells. Most species are saprophytic soil forms with only a few species being pathogenic.Calcium Sulfate: A calcium salt that is used for a variety of purposes including: building materials, as a desiccant, in dentistry as an impression material, cast, or die, and in medicine for immobilizing casts and as a tablet excipient. It exists in various forms and states of hydration. Plaster of Paris is a mixture of powdered and heat-treated gypsum.Lactobacillus brevis: A species of gram-positive, rod-shaped LACTIC ACID bacteria that is frequently used as starter culture in SILAGE fermentation, sourdough, and lactic-acid-fermented types of beer and wine.Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.Ultrafiltration: The separation of particles from a suspension by passage through a filter with very fine pores. In ultrafiltration the separation is accomplished by convective transport; in DIALYSIS separation relies instead upon differential diffusion. Ultrafiltration occurs naturally and is a laboratory procedure. Artificial ultrafiltration of the blood is referred to as HEMOFILTRATION or HEMODIAFILTRATION (if combined with HEMODIALYSIS).Magnesium: A metallic element that has the atomic symbol Mg, atomic number 12, and atomic weight 24.31. It is important for the activity of many enzymes, especially those involved in OXIDATIVE PHOSPHORYLATION.MethylaminesSulfuric Acid Esters: Organic esters of sulfuric acid.Centrifugation, Density Gradient: Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Ultracentrifugation: Centrifugation with a centrifuge that develops centrifugal fields of more than 100,000 times gravity. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Polysaccharide-Lyases: A group of carbon-oxygen lyases. These enzymes catalyze the breakage of a carbon-oxygen bond in polysaccharides leading to an unsaturated product and the elimination of an alcohol. EC 4.2.2.Paspalum: A plant genus of the family POACEAE that is used for forage.Nitrates: Inorganic or organic salts and esters of nitric acid. These compounds contain the NO3- radical.Penicillium: A mitosporic Trichocomaceae fungal genus that develops fruiting organs resembling a broom. When identified, teleomorphs include EUPENICILLIUM and TALAROMYCES. Several species (but especially PENICILLIUM CHRYSOGENUM) are sources of the antibiotic penicillin.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Immunoelectrophoresis: A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.Oxidation-Reduction: A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).Chloromercuribenzoates: Chloride and mercury-containing derivatives of benzoic acid.Protein Conformation: The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).Metals: Electropositive chemical elements characterized by ductility, malleability, luster, and conductance of heat and electricity. They can replace the hydrogen of an acid and form bases with hydroxyl radicals. (Grant & Hackh's Chemical Dictionary, 5th ed)Oligosaccharides: Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.Spectrophotometry, Ultraviolet: Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Cations, Divalent: Positively charged atoms, radicals or groups of atoms with a valence of plus 2, which travel to the cathode or negative pole during electrolysis.Sulfuric Acids: Inorganic and organic derivatives of sulfuric acid (H2SO4). The salts and esters of sulfuric acid are known as SULFATES and SULFURIC ACID ESTERS respectively.Edetic Acid: A chelating agent that sequesters a variety of polyvalent cations such as CALCIUM. It is used in pharmaceutical manufacturing and as a food additive.Chondroitinases and Chondroitin Lyases: Enzymes which catalyze the elimination of glucuronate residues from chondroitin A,B, and C or which catalyze the hydrolysis of sulfate groups of the 2-acetamido-2-deoxy-D-galactose 6-sulfate units of chondroitin sulfate. EC 4.2.2.-.Sulfate Adenylyltransferase: An enzyme that catalyzes the activation of sulfate ions by ATP to form adenosine-5'-phosphosulfate and pyrophosphate. This reaction constitutes the first enzymatic step in sulfate utilization following the uptake of sulfate. EC 2.7.7.4.Heparin: A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts.Methods: A series of steps taken in order to conduct research.Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.Carbohydrate Sequence: The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.Cytosol: Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.beta-Glucosidase: An exocellulase with specificity for a variety of beta-D-glycoside substrates. It catalyzes the hydrolysis of terminal non-reducing residues in beta-D-glucosides with release of GLUCOSE.Pronase: A proteolytic enzyme obtained from Streptomyces griseus.Cell-Free System: A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)Immune Sera: Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.Glucosidases: Enzymes that hydrolyze O-glucosyl-compounds. (Enzyme Nomenclature, 1992) EC 3.2.1.-.Urea: A compound formed in the liver from ammonia produced by the deamination of amino acids. It is the principal end product of protein catabolism and constitutes about one half of the total urinary solids.alpha-L-Fucosidase: An enzyme that catalyzes the hydrolysis of an alpha L-fucoside to yield an alcohol and L-fucose. Deficiency of this enzyme can cause FUCOSIDOSIS. EC 3.2.1.51.Swine: Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).Physicochemical Phenomena: The physical phenomena describing the structure and properties of atoms and molecules, and their reaction and interaction processes.Oxidoreductases: The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)Chemistry, Physical: The study of CHEMICAL PHENOMENA and processes in terms of the underlying PHYSICAL PHENOMENA and processes.Flavobacterium: A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in SOIL and WATER. Its organisms are also found in raw meats, MILK and other FOOD, hospital environments, and human clinical specimens. Some species are pathogenic in humans.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Chromatography, Agarose: A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million.Indicators and Reagents: Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Hydrolases: Any member of the class of enzymes that catalyze the cleavage of the substrate and the addition of water to the resulting molecules, e.g., ESTERASES, glycosidases (GLYCOSIDE HYDROLASES), lipases, NUCLEOTIDASES, peptidases (PEPTIDE HYDROLASES), and phosphatases (PHOSPHORIC MONOESTER HYDROLASES). EC 3.Sulfhydryl Reagents: Chemical agents that react with SH groups. This is a chemically diverse group that is used for a variety of purposes. Among these are enzyme inhibition, enzyme reactivation or protection, and labelling.Aminopeptidases: A subclass of EXOPEPTIDASES that act on the free N terminus end of a polypeptide liberating a single amino acid residue. EC 3.4.11.Electrophoresis: An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Acetates: Derivatives of ACETIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxymethane structure.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Mitosporic Fungi: A large and heterogenous group of fungi whose common characteristic is the absence of a sexual state. Many of the pathogenic fungi in humans belong to this group.Streptomyces: A genus of bacteria that form a nonfragmented aerial mycelium. Many species have been identified with some being pathogenic. This genus is responsible for producing a majority of the ANTI-BACTERIAL AGENTS of practical value.Chromatography, Thin Layer: Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Plants: Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.SulfatasesCation Transport Proteins: Membrane proteins whose primary function is to facilitate the transport of positively charged molecules (cations) across a biological membrane.Dialysis: A process of selective diffusion through a membrane. It is usually used to separate low-molecular-weight solutes which diffuse through the membrane from the colloidal and high-molecular-weight solutes which do not. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Molybdenum: A metallic element with the atomic symbol Mo, atomic number 42, and atomic weight 95.94. It is an essential trace element, being a component of the enzymes xanthine oxidase, aldehyde oxidase, and nitrate reductase. (From Dorland, 27th ed)Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Glycoproteins: Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.Adsorption: The adhesion of gases, liquids, or dissolved solids onto a surface. It includes adsorptive phenomena of bacteria and viruses onto surfaces as well. ABSORPTION into the substance may follow but not necessarily.Protamines: A group of simple proteins that yield basic amino acids on hydrolysis and that occur combined with nucleic acid in the sperm of fish. Protamines contain very few kinds of amino acids. Protamine sulfate combines with heparin to form a stable inactive complex; it is used to neutralize the anticoagulant action of heparin in the treatment of heparin overdose. (From Merck Index, 11th ed; Martindale, The Extra Pharmacopoeia, 30th ed, p692)Durapatite: The mineral component of bones and teeth; it has been used therapeutically as a prosthetic aid and in the prevention and treatment of osteoporosis.Agaricales: An extensive order of basidiomycetous fungi whose fruiting bodies are commonly called mushrooms.Vibrio: A genus of VIBRIONACEAE, made up of short, slightly curved, motile, gram-negative rods. Various species produce cholera and other gastrointestinal disorders as well as abortion in sheep and cattle.Detergents: Purifying or cleansing agents, usually salts of long-chain aliphatic bases or acids, that exert cleansing (oil-dissolving) and antimicrobial effects through a surface action that depends on possessing both hydrophilic and hydrophobic properties.Chondroitin Lyases: Enzymes which catalyze the elimination of delta-4,5-D-glucuronate residues from polysaccharides containing 1,4-beta-hexosaminyl and 1,3-beta-D-glucuronosyl or 1,3-alpha-L-iduronosyl linkages thereby bringing about depolymerization. EC 4.2.2.4 acts on chondroitin sulfate A and C as well as on dermatan sulfate and slowly on hyaluronate. EC 4.2.2.5 acts on chondroitin sulfate A and C.Protease Inhibitors: Compounds which inhibit or antagonize biosynthesis or actions of proteases (ENDOPEPTIDASES).Alcohol Oxidoreductases: A subclass of enzymes which includes all dehydrogenases acting on primary and secondary alcohols as well as hemiacetals. They are further classified according to the acceptor which can be NAD+ or NADP+ (subclass 1.1.1), cytochrome (1.1.2), oxygen (1.1.3), quinone (1.1.5), or another acceptor (1.1.99).Glutamate-Ammonia Ligase: An enzyme that catalyzes the conversion of ATP, L-glutamate, and NH3 to ADP, orthophosphate, and L-glutamine. It also acts more slowly on 4-methylene-L-glutamate. (From Enzyme Nomenclature, 1992) EC 6.3.1.2.Chickens: Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.Heparin Lyase: An enzyme of the isomerase class that catalyzes the eliminative cleavage of polysaccharides containing 1,4-linked D-glucuronate or L-iduronate residues and 1,4-alpha-linked 2-sulfoamino-2-deoxy-6-sulfo-D-glucose residues to give oligosaccharides with terminal 4-deoxy-alpha-D-gluc-4-enuronosyl groups at their non-reducing ends. (From Enzyme Nomenclature, 1992) EC 4.2.2.7.Trypsin: A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Immunochemistry: Field of chemistry that pertains to immunological phenomena and the study of chemical reactions related to antigen stimulation of tissues. It includes physicochemical interactions between antigens and antibodies.Manganese: A trace element with atomic symbol Mn, atomic number 25, and atomic weight 54.94. It is concentrated in cell mitochondria, mostly in the pituitary gland, liver, pancreas, kidney, and bone, influences the synthesis of mucopolysaccharides, stimulates hepatic synthesis of cholesterol and fatty acids, and is a cofactor in many enzymes, including arginase and alkaline phosphatase in the liver. (From AMA Drug Evaluations Annual 1992, p2035)Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Plant Proteins: Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.Fermentation: Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.Centrifugation: Process of using a rotating machine to generate centrifugal force to separate substances of different densities, remove moisture, or simulate gravitational effects. It employs a large motor-driven apparatus with a long arm, at the end of which human and animal subjects, biological specimens, or equipment can be revolved and rotated at various speeds to study gravitational effects. (From Websters, 10th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Apoenzymes: The protein components of enzyme complexes (HOLOENZYMES). An apoenzyme is the holoenzyme minus any cofactors (ENZYME COFACTORS) or prosthetic groups required for the enzymatic function.Peptides: Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.Phosphates: Inorganic salts of phosphoric acid.Microscopy, Electron: Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.Cell Fractionation: Techniques to partition various components of the cell into SUBCELLULAR FRACTIONS.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Endopeptidases: A subclass of PEPTIDE HYDROLASES that catalyze the internal cleavage of PEPTIDES or PROTEINS.IndophenolChromatography, Liquid: Chromatographic techniques in which the mobile phase is a liquid.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Phosphotransferases: A rather large group of enzymes comprising not only those transferring phosphate but also diphosphate, nucleotidyl residues, and others. These have also been subdivided according to the acceptor group. (From Enzyme Nomenclature, 1992) EC 2.7.AmidohydrolasesAminohydrolasesSepharosePeptide Fragments: Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.Flavin-Adenine Dinucleotide: A condensation product of riboflavin and adenosine diphosphate. The coenzyme of various aerobic dehydrogenases, e.g., D-amino acid oxidase and L-amino acid oxidase. (Lehninger, Principles of Biochemistry, 1982, p972)Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Arthrobacter: A genus of asporogenous bacteria isolated from soil that displays a distinctive rod-coccus growth cycle.Lyases: A class of enzymes that catalyze the cleavage of C-C, C-O, and C-N, and other bonds by other means than by hydrolysis or oxidation. (Enzyme Nomenclature, 1992) EC 4.Biological Assay: A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc.Sulfur Radioisotopes: Unstable isotopes of sulfur that decay or disintegrate spontaneously emitting radiation. S 29-31, 35, 37, and 38 are radioactive sulfur isotopes.Basidiomycota: A phylum of fungi that produce their sexual spores (basidiospores) on the outside of the basidium. It includes forms commonly known as mushrooms, boletes, puffballs, earthstars, stinkhorns, bird's-nest fungi, jelly fungi, bracket or shelf fungi, and rust and smut fungi.Streptococcus: A genus of gram-positive, coccoid bacteria whose organisms occur in pairs or chains. No endospores are produced. Many species exist as commensals or parasites on man or animals with some being highly pathogenic. A few species are saprophytes and occur in the natural environment.Enzyme Activation: Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.Phosphoadenosine Phosphosulfate: 3'-Phosphoadenosine-5'-phosphosulfate. Key intermediate in the formation by living cells of sulfate esters of phenols, alcohols, steroids, sulfated polysaccharides, and simple esters, such as choline sulfate. It is formed from sulfate ion and ATP in a two-step process. This compound also is an important step in the process of sulfur fixation in plants and microorganisms.Isoenzymes: Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.Pentosyltransferases: Enzymes of the transferase class that catalyze the transfer of a pentose group from one compound to another.Trimethyl Ammonium Compounds: QUATERNARY AMMONIUM COMPOUNDS containing three methyl groups, having the general formula of (CH3)3N+R.Indican: A substance occurring in the urine of mammals and also in blood plasma as the normal metabolite of tryptophan. An increased urinary excretion of indican is seen in Hartnup disease from the bacterial degradation of unabsorbed tryptophan.Enzymes: Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however CATALYTIC RNA and CATALYTIC DNA molecules have also been identified.Carrier Proteins: Transport proteins that carry specific substances in the blood or across cell membranes.Iduronic Acid: Component of dermatan sulfate. Differs in configuration from glucuronic acid only at the C-5 position.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Sulfur: An element that is a member of the chalcogen family. It has an atomic symbol S, atomic number 16, and atomic weight [32.059; 32.076]. It is found in the amino acids cysteine and methionine.Alpha-Globulins: Serum proteins that have the most rapid migration during ELECTROPHORESIS. This subgroup of globulins is divided into faster and slower alpha(1)- and alpha(2)-globulins.Seeds: The encapsulated embryos of flowering plants. They are used as is or for animal feed because of the high content of concentrated nutrients like starches, proteins, and fats. Rapeseed, cottonseed, and sunflower seed are also produced for the oils (fats) they yield.Chondroitin ABC Lyase: An enzyme that catalyzes the eliminative degradation of polysaccharides containing 1,4-beta-D-hexosaminyl and 1,3-beta-D-glucuronosyl or 1,3-alpha-L-iduronosyl linkages to disaccharides containing 4-deoxy-beta-D-gluc-4-enuronosyl groups. (Enzyme Nomenclature, 1992)Blood Proteins: Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins.Carbon: A nonmetallic element with atomic symbol C, atomic number 6, and atomic weight [12.0096; 12.0116]. It may occur as several different allotropes including DIAMOND; CHARCOAL; and GRAPHITE; and as SOOT from incompletely burned fuel.Carboxy-Lyases: Enzymes that catalyze the addition of a carboxyl group to a compound (carboxylases) or the removal of a carboxyl group from a compound (decarboxylases). EC 4.1.1.Lipase: An enzyme of the hydrolase class that catalyzes the reaction of triacylglycerol and water to yield diacylglycerol and a fatty acid anion. It is produced by glands on the tongue and by the pancreas and initiates the digestion of dietary fats. (From Dorland, 27th ed) EC 3.1.1.3.Hemagglutination: The aggregation of ERYTHROCYTES by AGGLUTININS, including antibodies, lectins, and viral proteins (HEMAGGLUTINATION, VIRAL).Trypsin Inhibitors: Serine proteinase inhibitors which inhibit trypsin. They may be endogenous or exogenous compounds.Blood Protein Electrophoresis: Electrophoresis applied to BLOOD PROTEINS.Toxins, Biological: Specific, characterizable, poisonous chemicals, often PROTEINS, with specific biological properties, including immunogenicity, produced by microbes, higher plants (PLANTS, TOXIC), or ANIMALS.

Cloning and characterization of a cDNA encoding a novel extracellular peroxidase from Trametes versicolor. (1/812)

The white rot basidiomycete Trametes versicolor secretes a large number of peroxidases which are believed to be involved in the degradation of polymeric lignin. These peroxidases have been classified previously as lignin peroxidases or manganese peroxidases (MnP). We have isolated a novel extracellular peroxidase-encoding cDNA sequence from T. versicolor CU1, the transcript levels of which are repressed by low concentrations of Mn2+ and induced by nitrogen and carbon but not induced in response to a range of stresses which have been reported to induce MnP expression.  (+info)

Separation and properties of two acetylacetoin reductases from Bacillus cereus YUF-4. (2/812)

The separation and purification of two kinds of acetylacetoin reductases (AACRs) from Bacillus cereus YUF-4 were examined. NADPH-linked AACR (AACR I) and NADH-linked AACR (AACR II) were separated from each other by ammonium sulfate fractionation, DEAE-cellulose chromatography, and hydroxyapatite chromatography. The former was purified 3.4-fold with a yield of 10.0%, and the latter was purified 29-fold with a yield of 15.6%. The two enzymes differ from each other in some enzymic properties such as substrate specificity.  (+info)

Purification of xyloglucan endotransglycosylases (XETs): a generally applicable and simple method based on reversible formation of an enzyme-substrate complex. (3/812)

We describe a novel and general, mechanism-based, method for purification of xyloglucan endotransglycosylases (XETs) from crude plant extracts. Putative isoforms, obtained by step-wise precipitation with (NH4)2SO4, were incubated with tamarind xyloglucan (approximately 1 MDa) to form stable xyloglucan-XET complexes with apparent molecular masses >500 kDa on gel-permeation chromatography (GPC). Subsequent addition of xyloglucan-derived oligosaccharides (a mixture of XET acceptor substrates) caused a shift in the GPC elution volume of the activity back to that expected of a approximately 32 kDa protein, presumably by completing the transglycosylation reaction and so freeing the enzyme from the xyloglucan (donor substrate). This simple two-step method enabled the isolation of each XET activity attempted [various (NH4)2SO4 cuts from extracts of cauliflower florets and mung bean seedlings], in pure form as judged by SDS/PAGE.  (+info)

Differences in phosphofructokinase regulation in normal and tumor rat thyroid cells. (4/812)

The kinetic and molecular properties of a phosphofructokinase derived from a transplantable rat thyroid tumor lacking regulatory control on the glycolytic pathway were studied. The properties of the near-purified enzyme (specific activity 140 units/mg) were compared with those of phosphofructokinase from normal rat thyroid (specific activity 134 units/mg). The electrophoretic mobilities and gel elution behavior of these two enzymes were almost similar. The thyroid tumor phosphofructokinase showed, however, a greater degree of size and/or shape heterogeneity in the presence of ATP than the normal thyroid enzyme, as determined by gel filtration and sucrose density gradient centrifugation. Kinetic studies below pH 7.4 showed a sigmoid response curve for both enzymes when the velocity was determined at 1 mM ATP with varying levels of fructose-6-P. The interaction coefficient, however, was 4.2 and 2.6 for normal and tumor thyroid phosphofructokinase, respectively. Ammonium sulfate decreased the cooperative interactions with the substrate fructose-6-P in both enzymes. The thyroid tumor enzyme, however, was less sensitive to the inhibition by ATP and by citrate. The reversal of citrate inhibition by cyclic 3':5'-adenosine monophosphate was also less effective with the thyroid tumor phosphofructokinase, while the protective effect of fructose-6-P was stronger. The difference in citrate inhibition between tumor and normal thyroid enzyme was not strongly affected by varying the MgCl2 concentration up to 10 mM. It is concluded that the complex allosteric regulation typical of the normal thyroid phosphofructokinase is still present in the enzyme isolated from the thyroid tumor tissue. The latter, however, is more loosely controlled by its physiological effectors, such as ATP, citrate, and cyclic AMP.  (+info)

Magnesium-dependent folding of self-splicing RNA: exploring the link between cooperativity, thermodynamics, and kinetics. (5/812)

Folding of the Tetrahymena self-splicing RNA into its active conformation involves a set of discrete intermediate states. The Mg2+-dependent equilibrium transition from the intermediates to the native structure is more cooperative than the formation of the intermediates from the unfolded states. We show that the degree of cooperativity is linked to the free energy of each transition and that the rate of the slow transition from the intermediates to the native state decreases exponentially with increasing Mg2+ concentration. Monovalent salts, which stabilize the folded RNA nonspecifically, induce states that fold in less than 30 s after Mg2+ is added to the RNA. A simple model is proposed that predicts the folding kinetics from the Mg2+-dependent change in the relative stabilities of the intermediate and native states.  (+info)

Selective peroxisome degradation in Yarrowia lipolytica after a shift of cells from acetate/oleate/ethylamine into glucose/ammonium sulfate-containing media. (6/812)

We have shown that peroxisomes of the yeast Yarrowia lipolytica are subject to specific degradation after exposure of acetate/oleate-grown cells to glucose excess conditions. Electron microscopic analysis has revealed that the peroxisomes were degraded by uptake in the vacuole. Our results suggest that peroxisomes are taken up by macroautophagic processes, because sequestration of individual peroxisomes, which occurs typically at the beginning of microautophagy, was never observed. The observation that a peroxisomal amine oxidase activity is specifically induced by ethylamine was used for the development of a plate assay screening procedure to isolate peroxisome degradation-defective mutants.  (+info)

Complementary effects of bifidogenic growth stimulators and ammonium sulfate in natural rubber serum powder on Bifidobacterium bifidum. (7/812)

Natural rubber serum powder, rich in crude protein and carbohydrates, had a strong growth-stimulating activity for Bifidobacterium bifidum JCM 1254, which was unable to grow in a fully synthetic medium, B12 assay medium. Natural rubber serum powder was fractionated by ultrafiltration (molecular weight cutoff 1000). The active ultrafiltrate was further concentrated and desalted with an adsorptive microconcentrator, which adsorbs virtually all amino acids and peptides. Through this purification step, it was found that the adsorbed fraction obtained did not stimulate growth independently but acted complementarily with a small amount of ammonium sulfate. The adsorbed fraction was subsequently analyzed on reversed-phase high pressure liquid chromatography, and the activities of the eluates were measured on B12 assay medium with ammonium sulfate. Consequently, it was proved that several peptidic ingredients in the adsorbed fraction increased the growth of B. bifidum.  (+info)

Stimulation of peroxidase activity by decamerization related to ionic strength: AhpC protein from Amphibacillus xylanus. (8/812)

AhpC protein, purified from Amphibacillus xylanus with a molecular mass of 20.8 kDa, protects cells against oxidation damage. The enzyme catalyses the reduction of hydroperoxides in cooperation with the 55 kDa flavoprotein, A. xylanus NADH oxidase (NADH oxidase-AhpC system). A. xylanus AhpC has two disulfide linkages between monomers and can act in the homodimer form. Gel-filtration column chromatography and dynamic light scattering (DLS) suggest that A. xylanus AhpC also forms a large oligomeric assembly (10-12 mers). A. xylanus AhpC was crystallized and X-ray diffraction data were collected to 3.0 A. The self-rotation function revealed fivefold and twofold axes located perpendicularly to each other, suggesting that the molecular assembly of A. xylanus AhpC is composed of ten monomers. The oligomerization of A. xylanus AhpC is affected by ionic strength in the DLS measurements. The H(2)O(2) reductase activity of the A. xylanus NADH oxidase-AhpC system is also affected by ionic strength, and it was found that the decamerization of AhpC might be required for the activation of the NADH oxidase-AhpC system.  (+info)

*Ammonium sulfate

Double metal sulfates include ammonium cobaltous sulfate, ferrous diammonium sulfate, ammonium nickel sulfate which are known ... Ammonium sulfate is used on a small scale in the preparation of other ammonium salts, especially ammonium persulfate. Ammonium ... The filtrate on evaporation yields ammonium chloride. Ammonium sulfate forms many double salts (ammonium metal sulfates) when ... Anhydrous double sulfates of ammonium also occur in the Langbeinites family. In November 2009, a ban on ammonium sulfate, ...

*Ammonium sulfate precipitation

Ammonium sulfate is an inorganic salt with a high solubility that disassociates into ammonium (NH4+) and sulfate (SO42−) in ... Ammonium sulfate precipitation is one of the most commonly used methods for large and laboratory scale protein purification and ... Ammonium sulfate is especially useful as a precipitant because it is highly soluble, stabilizes protein structure, has a ...

*Ammonium lauryl sulfate

... (ALS) is the common name for ammonium dodecyl sulfate (CH3(CH2)10CH2OSO3NH4). The molecule consists of ... Sodium lauryl sulfate Sodium laureth sulfate Potassium lauryl sulfate Sodium pareth sulfate "Household Products Database - ... Ammonium Lauryl Sulfate". Retrieved 2007-01-25. "MSDS for ammonium lauryl sulfate" (PDF). Archived from the original (PDF) on ... "Final Report on the Safety Assessment of Sodium Lauryl Sulfate and Ammonium Lauryl Sulfate". JACT, CIR publication. 2 (7): pp. ...

*Ammonium aluminium sulfate

... , also known as ammonium alum or just alum, is a white crystalline double sulfate usually encountered ... Ammonium alum is made from aluminium hydroxide, sulfuric acid and ammonium sulfate. It forms a solid solution with potassium ... Aluminium sulfate, closely related to ammonium alum, is considered nontoxic up to LD50 of 6207 mg/kg. For reduced ... Aluminum Ammonium Sulfate Material Safety Data Sheet, J. T. Baker, March 2009, retrieved 2009-11-23 . D-TER Animal and Bird ...

*Ammonium cerium(IV) sulfate

... is an inorganic compound with the formula (NH4)4Ce(SO4)4.2 H2O. It is an orange-colored solid. It ... where the cerium atoms are 9 coordinated by oxygen atoms belonging to sulfate groups, in a distorted tricapped trigonal prism. ... is a strong oxidant, the potential for reduction is about +1.44V. Cerium(IV) sulfate is a related compound. A crystallographic ...

*Ammonium iron(II) sulfate

Like the other ferrous sulfate salts, ferrous ammonium sulfate dissolves in water to give the aquo complex [Fe(H2O)6]2+, which ... Containing two different cations, Fe2+ and NH4+, it is classified as a double salt of ferrous sulfate and ammonium sulfate. It ... Mohr's salt is prepared by dissolving an equimolar mixture of hydrated ferrous sulfate and ammonium sulfate in water containing ... Ferrous ammonium sulfate forms light green crystals. Common impurities include Mg, Mn, Ni, Pb, and Zn, many of which form ...

*Ammonium iron(III) sulfate

The ferric sulfate is mixed with ammonium sulfate and crystallized to get ammonium iron(III) sulfate. The solution is normally ... ferrous ammonium sulfate. FAS can be prepared by crystallization from a solution of ferric sulfate and ammonium sulfate. Iron( ... Ammonium iron(III) sulfate, NH4Fe(SO4)2·12 H2O, or NH4[Fe(H2O)6](SO4)2·6 H2O, also known as ferric ammonium sulfate (FAS) or ... Crystals of ferric ammonium sulfate Crystals of ammonium iron(III) sulfate after 16 days in the air Considine, Douglas M: ...

*Tetraamminecopper(II) sulfate

Most pesticides contain ammonium sulfate. Ammonium sulfate is used as an agricultural spray adjuvant for water-soluble ... sulfate. The magnetic and thermal properties of tetraammine copper (II) sulfate monohydrate have been extensively studied and ... Tetraamminecopper(II) sulfate is the inorganic compound with the formula [Cu(NH3)4(H2O)n]SO4. This dark blue solid is a metal ... The brilliant dark blue-violet color of tetraamminecopper(II) sulfate solution is due to presence of [Cu(NH3)4]2+. Often, the ...

*Tutton's salt

Magnesium ammonium sulfate hexahydrate and nickel ammonium sulfate hexahydrate". Acta Crystallographica. International Union of ... The Tutton's salts were once termed "false alums". Unit cell of ferrous ammonium sulfate (N is violet, O is red, S is orange, ... Perhaps the best-known is Mohr's salt, ferrous ammonium sulfate (NH4)2Fe(SO4)2.(H2O)6). Other examples include the vanadous ... V. Manganese ammonium sulfate hexahydrate". Acta Crystallographica. 20 (6): 731-733. doi:10.1107/S0365110X66001762. ISSN 0365- ...

*Giovanni Francisco Vigani

Among other things, Vigani devised a method for purifying iron sulphate[disambiguation needed] from copper; for making ammonium ... sulphate; and for proving that to form a given salt a metallic base takes always the same amount of acid. He also invented a ...

*Cerium(IV) sulfate

A related compound is ceric ammonium sulfate. The solubility of Ce(IV) in methanesulfonic acid is approximately 10 times the ... Cerium(IV) sulfate, also called ceric sulfate, is an inorganic compound. It exists as the anhydrous salt Ce(SO4)2 as well as a ... ISBN 0-8493-0487-3. Mariappan Periasamy, Ukkiramapandian Radhakrishnan "Cerium(IV) Ammonium Sulfate" Encyclopedia of Reagents ... Solutions of ceric sulfate have a strong yellow color. The tetrahydrate loses water when heated to 180-200 °C. The ceric ion is ...

*Detergent

... the cationic surfactants have quaternary ammonium as the polar end. The ammonium sulfate center is positively charged. Non- ...

*Protein precipitation

The most commonly used salt is ammonium sulfate. There is a low variation in salting out over temperatures 0 °C to 30 °C. ... Addition of a neutral salt, such as ammonium sulfate, compresses the solvation layer and increases protein-protein interactions ...

*Salting out

Duong-Ly, Krisna (25 March 2014). "Salting out of Proteins Using Ammonium Sulfate Precipitation". Methods in Enzymology: 85-94 ... Ionic strength Protein precipitation Salting in Ammonium sulfate precipitation Hofmeister series Genck, Wayne. "Make The Most ...

*Gas Works Park

... ammonium sulfate, and sulfuric acid; and toluene for use in explosives. Toluene was in high demand during World War II, and ...

*Candle

Typical agents are ammonium nitrate and ammonium sulfate. Based on measurements of a taper-type, paraffin wax candle, a modern ...

*Mascagnite

... is a rare ammonium sulfate mineral (NH4)2SO4. It crystallizes in the orthorhombic system typically forming as ...

*Chemische Fabrik Kalk

... ammonium hydroxide, ammonium chloride, ammonia, sodium hydroxide, sodium sulfate, nitric acid, hydrochloric acid, sulfuric acid ... In March 1946 the production of ammonium sulfate resumed. In 1947 large parts of the factory were rebuilt and in the summer of ... One year later the production of the fertilizer ammonium sulfate begins. In 1866 the factory begins to use the Leblanc process ... The company continued to expand, with a factory producing ammonium sulfate opening in 1865 in Raderberg near Cologne ( ...

*Selective catalytic reduction

Other issues that must be considered in using SCR for NO x control in power plants are the formation of ammonium sulfate and ... Other possible reductants include cyanuric acid and ammonium sulfate. SCR catalysts are made from various ceramic materials ... Some examples of plugging contaminates are: fine particulate, ammonia sulfur compounds, ammonium bisulfate (ABS) and silicon ... ammonium bisulfate due to the sulfur content of the fuel as well as the undesirable catalyst-caused formation of SO 3 from the ...

*Bismuth phosphate process

The plutonium was reduced again by adding ferrous ammonium sulfate. Bismuth nitrate and phosphoric acid were added and bismuth ... sulfates and ammonium nitrate were added to the solution and the hexavalent plutonium was precipitated as plutonium peroxide. ...

*Jari project

Ludwig corrected the situation with the application of ammonium sulfate. He expanded to cattle farming and also discovered a ...

*Vanadium(II) sulfate

A related salt is vanadous ammonium sulfate, (NH4)2V(SO4)2·6H2O, a Tutton's salt isomorphous with ferrous ammonium sulfate. ... Vanadium(II), iron(II) and cobalt(II) ammonium sulfate hexahydrates". Acta Crystallographica. 22 (6): 775-780. doi:10.1107/ ... Vanadium(II) sulfate describes a family of inorganic compounds with the formula VSO4(H2O)x where 0 ≤ x ≤ 7. The hexahydrate is ... Kranz, M.; Goldberg, D. E.; Ribner, A. (1963). "Vanadium(II) Sulfate". Inorganic Syntheses. 7: 94-96. doi:10.1002/9780470132388 ...

*Titan (moon)

Titan is resurfaced through the process by grain-sized ice and ammonium sulfate ash, which helps produce a wind-shaped ... Fortes, A. D.; Grindroda, P.M.; Tricketta, S. K.; Vočadloa, L. (May 2007). "Ammonium sulfate on Titan: Possible origin and role ... The low-pressure ice, overlaying a liquid layer of ammonium sulfate, ascends buoyantly, and the unstable system can produce ...

*Lead-acid battery

Surplus ammonia and water evaporate, leaving an ammonium sulfate residue. Sodium bicarbonate (baking soda) is also commonly ... In addition, the sulfate portion (of the lead sulfate) is not returned to the electrolyte as sulfuric acid. It is believed that ... White corrosion is usually lead or zinc sulfate crystals. Aluminum connectors corrode to aluminum sulfate. Copper connectors ... react with sulfuric acid in the electrolyte to form lead sulfate. The lead sulfate first forms in a finely divided, amorphous ...

*Cellulose insulation

Many cellulose companies use a blend of ammonium sulfate and borate for fire retardation. Although ammonium sulfate is normally ... The other 15% is a fire retardant such as boric acid or ammonium sulphate. Cellulose has the highest recycled content of any ... Many cellulose companies use a blend of ammonium sulfate and borate. A vapor barrier may not be needed with cellulose ...

*List of food additives

... mineral salt Ammonium phosphatides - emulsifier Ammonium polyphosphates - anti-caking agent Ammonium sulfate - mineral salt, ... food acid Ammonium fumarate - food acid Ammonium hydroxide - mineral salt Ammonium lactate - food acid Ammonium malate - food ... emulsifier Ammonium bicarbonate - mineral salt Ammonium carbonate - mineral salt Ammonium chloride - mineral salt Ammonium ... Aluminium - color (silver) Aluminium ammonium sulfate - mineral salt Aluminium potassium sulfate - mineral salt Aluminium ...
Definition of Ammonium sulphate precipitation with photos and pictures, translations, sample usage, and additional links for more information.
I need to produce amylase immobilized from bacillus subtilis. Before that, i have to do ammonium sulphate precipitation. I add the ammonium sulphate 4.5g (30 saturated) into 20ml of amylase produced at 4 degree celcius and centrifuge it. But, there is no pellet found and I cant proceed to immobilization. I am very worry as i have to finish my project in one month. I need some advice. ...
Before Its News). Ammonium Sulphate market analysis report speaks about the manufacturing process. The process is analysed thoroughly with four points Manufacturers, regional analysis, Segment by Type & Applications and the actual process of whole Ammonium Sulphate industry.. A complete analysis of the competitive landscape of the Ammonium Sulphate Market is provided in the report. This section includes company profiles of market key players. The profiles include contact information, gross, capacity, product details of each firm, price, and cost of Ammonium Sulphate Industry are covered.. Get Sample PDF of Ammonium Sulphate Market [email protected] http://www.360marketupdates.com/enquiry/request-sample/10367904 Ammonium sulfate is an inorganic chemical compound with the chemical formula (NH4)2SO4. It contains 21 percent nitrogen in the form of ammonium ions and 24 percent sulfur as sulfate ions. The purified material takes the form of white granules or crystals. It is commonly used as a fertilizer and as ...
Ammonium Sulfate Factory - Select 2017 high quality Ammonium Sulfate Factory products in best price from certified Chinese Ammonium Sulfate Granular manufacturers, Ammonium Sulfate Crystals suppliers, wholesalers and factory on Made-in-China.com
Persulfate Market by Type (Ammonium Persulfate, Sodium Persulfate, and Potassium Persulfate) & Application (Polymers, Electronics, Cosmetics & Personal Care, Pulp, Paper and Textile, Water Treatment - Market research report and industry analysis - 9384172
Ammonium sulfate precipitaion problem - posted in Molecular Biology: Hi, I cannot fractionate transgene protein from plant protein by ammonium sulfate precipitation. The total protein is extracted from leave by TCA precipitation. In order to identify the protein of interest, the western analysis is performed. I can see the expected protein ban but it is hard to see. I cannot increase the amount of the protein in the SDS PAGE because the gel is overloaded. So I think ammonium sulfate can he...
When an aqueous solution of lithium chloride is mixed with an aqueous solution of ammonium sulfate a precipitate forms a new salt is formed a gas is evolved an acid and base are formed no reaction occurs I either thought it was a precipitate ...
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Ammonium sulfate, (NH4)2SO4 is an inorganic salt with a number of commercial uses. The primary use of ammonium sulfate is as a fertilizer for alkaline soils. It is also used as an agricultural spray adjuvant for water-soluble insecticides, herbicides, and fungicides. There, it functions to bind iron and calcium cations that are present in both well water and plant cells. ...
Ammonium sulfate, (NH4)2SO4 is an inorganic salt with a number of commercial uses. The primary use of ammonium sulfate is as a fertilizer for alkaline soils. It is also used as an agricultural spray adjuvant for water-soluble insecticides, herbicides, and fungicides. There, it functions to bind iron and calcium cations that are present in both well water and plant cells. ...
China Industry Ammonium Sulfate manufacturers - Select 2017 high quality Industry Ammonium Sulfate products in best price from certified Chinese Industry Oil manufacturers, Industry Grade suppliers, wholesalers and factory on Made-in-China.com
Aluminum ammonium sulfate definition, a crystalline solid, AlNH 4 (SO 4) 2 ⋅12H 2 O, used chiefly as a size in the manufacture of paper; alum. See more.
Furhter to the sensible sounding advice of using a simple Ammonium Sulphate precipiation in conjunction with a nickel column His.Tag purification, can anybody give me advice on how to set yourself up for such a step. Whats the easiest and most systematic way of determining what percentages of Ammonium Sulphate are needed for a particular protein; come to think of it, how exactly do you do the precipitation? Is it best before or after the nickel column? Thanks in advance, Jared -- Jared Head at the Department of Biochemistry, University of Bristol A computer lets you make more mistakes faster than any invention in human history - with the possible exceptions of handguns and tequila. Mitch Ratliffe ...
Press Release issued Oct 12, 2017: Future Market Insights (FMI) delivers key insights on the global and Far East & Baltic nations ammonium sulphate market in its upcoming report titled, Ammonium Sulphate Market: Global Industry Analysis and Opportunity Assessment, 2015-2025. The global ammonium sulphate market is projected to expand at a CAGR of 4.0% in terms of value during the forecast period owing to various factors, regarding which FMI offers vital insights in detail in this report.
Sigma-Aldrich offers Sigma-79688, Ammonium sulfate 0.2M - MES pH 6.5; 0.1M - PEG MME 5000 30% (w/v) solution for your research needs. Find product specific information including CAS, MSDS, protocols and references.
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The growing production of caprolactum in Asia Pacific, potential for interference in the ammonium sulfate industry is anticipated to be significant over the next few years.
Hi Guys, I am expressing the recombinant protein 6xHis tagged at C terminal secreting in YP-Galactose media by K lactis. My problem is whenever i try to purify by using Ni-NTA column, it turns the column white as if supernatant is chelating Ni from the column. I tried to condition supernatant by adjusting pH 8.0 and Salt upto 1M with no success. Please suggest me what should i do. Ammonium sulphate precipitation result in inactivation of protein. i cant do dialysis economically to huge culture volume. so these will not be a good option for conditioning media. Thanks ...
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Dr. bob could you please check this work cus i cant seem to get it right the first step of the synthesis is described by the reaction below. When 1.750 g of Fe(NH4)2(SO4)2 6H2O is mixed with 13 mL of 1.0 M H2C2O4, the theoretical ...
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And Others………. The report provides a basic overview of the Europe Ammonium Sulphate industry including definitions, segmentation, applications, key vendors, market drivers and market challenges. The Europe Ammonium Sulphate Market analysis is provided for the international markets including development trends, competitive landscape analysis, and key regions development status. Through the statistical analysis, the report depicts the global Europe Ammonium Sulphate Market including capacity, production, production value, cost/profit, supply/demand and import/export. The total market is further divided by company, by country, and by application/type for the competitive landscape analysis.. Table of Contents. Chapter One Europe Ammonium Sulphate Industry Overview. • Europe Ammonium Sulphate Definition(Product Picture and Specifications). • Europe Ammonium Sulphate Classification and Application. • Europe Ammonium Sulphate Industry Chain Structure. • Europe Ammonium Sulphate Industry ...
LONDON - As one of the first and most widely used nitrogen fertilizers, ammonium sulfate is used mainly to supply various nutritional chemicals to growing plants. Proper usage of ammonium sulfate fertilizer helps to maintain healthy nitrogen levels and pH balance in soils. However, when used in excess it could cause a number of undesirable plant conditions.. In the years to come, the global market for ammonium sulfate fertilizer is set to keep on gaining traction, spurred by soaring demand for fertilizers on the back of constantly rising population along with decreasing arable lands. Moreover, shift in trend towards rising crop yield by enhancing nutrient contents in soil is also predicted to give a boost to the ammonium sulfate market growth.. Meantime, severe regulatory guidelines associated with the adoption of ammonium sulfate in countries like Germany, France, the UK and the USA are likely to significantly restrain the market growth in the offing.. Regionally, APAC dominates the worlds ...
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0070] Phenyl Sepharose® HP hydrophobic interaction resin (GE Healthcare) and a Chromaflow® Acrylic chromatography column (GE Healthcare) were utilized for this process step. The phenyl column was 60 cm in diameter with a target height of 15±1 cm (bed volume 42.4 L). The loading limit for the column was 40 grams of sample per liter of Phenyl Sepharose® HP resin. The step was performed at 17±2° C., and at a flow rate of 75 cm/hr. The load material was warmed, when required, to 17±2° C. prior to the start of the first cycle. The column was pre-washed with water and equilibrated with 1.0 M ammonium sulfate and 18 mM sodium phosphate, pH 7.0. Following equilibration, the column was loaded with the diluted phenyl load. After loading, the column was washed to baseline absorbance (A280) with 1.1 M ammonium sulfate and 20 mM sodium phosphate, pH 7.0, followed by 0.95 M ammonium sulfate and 17 mM sodium phosphate, pH 7.0, respectively. The product was eluted from the column at a reduced flow rate ...
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7.3. Ammonium sulphate demand forecast to 2021. 8. AMMONIUM SULPHATE SUPPLIERS IN MEXICO. 9. CONSUMERS OF AMMONIUM SULPHATE IN MEXICO. Please note that Mexico Ammonium Sulphate Market Outlook 2017 is a half ready publication and contents are subject to changes and additions. We have all data necessary for report preparation but they need to be retrieved from our databases, organized in a report, updated with the latest information and thus the complete study will be presented. This process takes 2-3 business days after the order is placed. Thus, our clients always get a revised and updated version of the report. *Where available for a company. ...
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Caprolactam grade ammonium sulfate granular: Nitrogen:21%min Moisture:0.3%max Free Acid:0.05%max Caprolactam grade ammonium sulfate granular: Nitrogen:21%min Moisture:0.3%max Free Acid:0.05%max 7783-20-2 Mainly using as fertilizer ammonium...
Ammonium Sulphate (AS) is an inorganic salt which is commonly use as soil fertilizer for alkaline soils to lower pH balance of soil, while contributing essential nitrogen for plant growth. This material is a by-product of the facility ammonia scrubbers, which remove levels of airborne ammonia from the compost tunnel air exhaust stream. This ammonium sulphate has fertilizer properties and Orgaworld London has registered this product with the Canadian Food Inspection Agency (CFIA) as a fertilizer. In 2016 the London facility produced 1,986 tonnes of ammonium sulphate.. ...
Aluminum Ammonium Sulfate containing medications, Aluminum Ammonium Sulfate indications and usages ATC and ICD codes, combinations with other active ingredients and trade names information from Drugs-about.com
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Decyl poly(oxyethylene) ether ammonium sulfate | C12H29NO5S | CID 171151 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.
Preparation and standardisation of 0.1 M Ammonium Thiocyanate solution using silver nitrate and Ferric Ammonium Sulphate solution as indicator used in chemical analysis of pharmaceutical ingredients.
Worthington Galactosidase, Beta, Purified [WLS004102-BULK] - UNSPSC Code(s):12352204≥300 units per mg proteinChromatographically purified. A suspension in 1.6 M ammonium sulfate.
Please note that Poland Ammonium Sulphate Market Outlook 2017 is a half ready publication and contents are subject to changes and additions. We have all data necessary for report preparation but they need to be retrieved from our databases, organized in a report, updated with the latest information and thus the complete study will be presented. This process takes 2-3 business days after the order is placed. Thus, our clients always get a revised and updated version of the report ...
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Preparation of human Ln-γ2 protein. Human Ln-5 and monomeric Ln-γ2 proteins were purified from culture medium of human gastric carcinoma STKM-1 and malignant melanoma Mum2B cells, respectively, as described previously ( 13).. Construction and expression of DIII, DIII/V, and DI/II. The cDNA of human Ln-γ2 was used as a template to generate DNA fragments encoding DI/II (nucleotides 1830-3580), DIII (nucleotides 1303-1737), and DIII/V (nucleotides 1-1820) by PCR. These PCR products were subcloned into the pcDNA3.1+ expression vector (Invitrogen), and their nucleotide sequences were confirmed. For transient expression of the encoded proteins, each expression plasmid (4 μg) was transfected into COS7 cells (100 mm dish) using Fugene 6 (Roche Diagnostics) and cells were cultured for 48 h in serum-free DMEM after 12 h of transfection. Conditioned medium was collected and concentrated by ammonium sulfate precipitation. Precipitates containing recombinant proteins were collected and dissolved in 20 ...
Metall : Fachzeitschrift für Metallurgie ; Technik, Wissenschaft, Wirtschaft. - Clausthal-Zellerfeld : GDMB-Informationsges, ISSN 0026-0746, ZDB-ID 2077589. - Vol. 55.2001, 11, p. 436-634 ...
Ric: You are SO right. The fungal fighting of the potassium sulfate and IF using ammonium sulfate as a Nitrogen source, far outweigh other...
[115 Pages Report] Check for Discount on United States Ammonium Silicofluoride Market Report 2016 report by QYResearch Group. Notes: Sales, means the sales volume of Ammonium Silicofluoride Revenue,...
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In order to detect the expression of the target genes in leukocytes and to optimize the reaction conditions for real-time quantitative PCR, reverse transcriptase PCR (RT-PCR) was performed using specific primer sets (Table 1). cDNA synthesis was performed using the iScriptTM cDNA Synthesis kit (Bio-Rad). The PCR mixture contained 1× PCR buffer (16.6 mM ammonium sulfate, 67 mM Tris, pH 8.8, 6.7 mM MgCl2, 10 mM 2-mercaptoethanol), dNTPs (each at 1.25 mM), primer pairs (100 pM each per reaction), and 10 ng of cDNA template in a final volume of 50 µl. Reactions were hot-started at 95 °C for 5 min before adding 1.5 units of Taq polymerase (Red-Hot®, Thermo Fisher Scientific, ABgene product line) at the annealing temperature of 56 °C, followed by polymerization at 72 °C for 1 min. Amplification was carried out in DNA Thermal Cycler TC480 (Perkin Elmer, Waltham, MA) for 45 cycles (denaturation for 45 s at 95 °C, annealing for 45 s at 56 °C, and polymerization at 72 °C for 30 s), followed by a ...
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Classification: Nitrogen Fertilizer Type:Ammonium Sulphate CAS No.: 7783-20-2 Other Names: ammonium sulfate MF: (NH4)2SO4 Place of Origin:China (Mainland) Release Type: Quick Purity: 21.0% min Application:Agricultural Industry 1.The components of...
Polyoxyethylene Allyloxy Nonylphenoxypropyl Ether Ammonium Sulfate - Surfactants - SAAPedia - SAAPedia, Detailed description of the surfactant characteristics, uses - Page1
Research Report on Global and China Ammonium sulfate Industry Professional Market Report 2017. The Report includes market price, demand, trends, size, Share, Growth, Forecast, Analysis & Overview.
Ynthetic crystals of nickel ammonium sulphate (NiSO4(NH4)2.6H2O) Such crystals are made by precipitation from a solution of nickel in sulphuric acid. In this example the crystal precipitate is realized on a rock subsurface to suggest a natural origin of the crystals. Actual width of sample is 230 mm - Stock Image C026/8100
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Customer: United Farmers Co-Operative Plant Location: Perth, WA, Australia Industry: Fertilizers Major Equipment: Compactor Material Handled: Ammonium Sulf
16:08, 2 August 2018‎ Mabus (Talk , contribs)‎ . . (4,331 bytes) (+4,331)‎ . . (Created page with {{Chembox , Name = Sodium persulfate , Reference = , IUPACName = Sodium peroxydisulfate , PIN = , SystematicName = , OtherNames = Sodium peroxodisulfate,br,Sodium peroxodisulp...) ...
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globulin definition: some of a class of proteins that are found thoroughly in bloodstream plasma, milk, muscle, and plant seeds which are insoluble in clear water and 1 / 2 saturated ammonium sulfate,…
1. The two subunits α and β of Halobacterium cutirubrum DNA-dependent RNA polymerase are required in equimolar amounts for RNA synthesis to occur in vitro at the maximum rate. 2. In the absence of bivalent cations no interaction occurs between α and β subunits or between the subunits and DNA. 3. Mn2+ causes the subunits to form a 1:1 complex that still does not bind to the template. 4. Mg2+ permits binding of the Mn2+-mediated complex to DNA. 5. The complete enzyme, αβ, is inhibited by rifampicin and only the β subunit relieves the inhibition when added in excess. 6. Rifampicin-insensitive, template-dependent RNA synthesis occurs in the presence of protein α alone provided an oligonucleotide with a 5′-purine terminus is supplied as primer. 7. In the primed reaction with the α protein and an oligonucleotide, the template specificity is independent of the ionic strength, in contrast with the marked effect of salt concentration on the template specificity of the complete enzyme. 8. It is ...
The production of ammonia via the Haber-Bosch process consumes large amounts of fossil fuels and releases large amounts of greenhouse gases. Ammonia has many important applications including fertilizer for crops and for microalgae-derived biofuel systems. Aquatic cyanobacteria fix nitrogen from the air and have been mass-cultured for many uses. This study analyzes, on a life cycle basis, a process to culture the cyanobacterium, Anabaena sp. ATCC 33047, in open ponds; harvest the biomass and exopolysaccharides and convert these to biogas; strip and convert the ammonia from the biogas residue to ammonium sulfate; dry the ammonium sulfate solution to ammonium sulfate crystals; transport the ammonium sulfate and convert it to liquid ammonia and concentrated sulfuric acid. When compared to the ammonia produced via the Haber-Bosch process, savings of about 1.0 × 105 MJ of non-renewable energy and 3100 kg CO2 equivalent of global warming potential per 1000 kg of liquid ammonia might be possible. The ...
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The report generally describes cerium ammonium sulfate (tetrahydrate), examines its uses, production methods, patents. Cerium ammonium sulfate (tetrahydrate)
Application: ELISA, In vitro neutralization and Western blot analysis. Isotype: Rabbit Ig. Antigen: For immunization recombinant mouse TNF-alpha (E.coli-derived) is used. Purification: Antibodies were sequentially purified by ammonium sulphate precipitation and protein A-affinity chromatography. Packaging: Lyophilized and vacuum-packed. Contents: 0.5 mg/vial. Buffer: Prior to lyophilization: 0.5 ml PBS + 125 mM trehalose. Specificity: Binds with high affinity to and efficiently neutralizes both natural and recombinant mouse TNF-alpha. High degree of cross-neutralizing activity with rat TNF-alpha.
0.1M ammonium sulfate, 24-32% PEG5000 monomethyl ether, 1mM ATP, 100mM Hepes buffer, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature ...
Preparation and standardisation of 0.1 M Ceric Ammonium Sulphate solution using Arsenic Trioxide and Ferrroin Sulphate solution as indicator used in testing of pharmaceutical substances.
Synonyms for ammonium lactate in Free Thesaurus. Antonyms for ammonium lactate. 1 synonym for ammonium: ammonium ion. What are synonyms for ammonium lactate?
Description:. ProScape® 19-0-19 3% Fe 62% MESA® is an ideal fertilizer for providing outstanding color and extended feeding. This 1:1 ratio of nitrogen and potassium provides higher potassium levels to improve leaf structure for better mowing and turf tolerance to disease and other stresses. Iron combined with ammonium sulfate provides exceptional dark, green color. MESA® delivers ammonium sulfate and Meth-Ex® 40 in a single, non-burning homogeneous particle. An excellent choice for all turf types where fast, safe green-up and consistent long-term feeding is desired. Guaranteed Analysis: ...
Lists the various brand names available for medicines containing ammonium lactate. Find information on ammonium lactate use, treatment, drug class and molecular formula.
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Comprehensive supplier list for METHYLTRIS[2-[(1-OXOOCTADECYL)OXY]ETHYL]AMMONIUM METHYL SULFATE,METHYLTRIS[2-OCTADECANOYLETHYL]AMMONIUM HYDROGEN SULFATE
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SGN Size: 220. Description:. ProScape® 16-25-12 25% MESA® N:P:K ratio is ideal for overseeding and new turf establishment. When used as a starter fertilizer, the slow release nitrogen provides feeding throughout the early period of turf development. The higher nitrogen rate also makes 16-25-12 an ideal analysis for over-seeding existing turf where there is mature turf in the stand that requires higher nitrogen for development. MESA® combines Meth-Ex 40® methylene urea with ammonium sulfate resulting in quick response, excellent color, long lasting, consistent results without a flush of growth or excessive clippings.. Guaranteed Analysis:. ...
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Physician reviewed ammonium lactate and halobetasol topical kit patient information - includes ammonium lactate and halobetasol topical kit description, dosage and directions.
You had to look at the graphs, graph A showed that the crop yield was increasing up until 200kg of ammonium and after that there was only a small increase so it wasnt worth it. Graph B showed that below 200kg ammonium there was always a profit, after 200kg the profit decreased. And finally graph c showed that above 300kg ammonium there would be a run off of ammonium meaning that it would be a waste ...
Accepted name: DNA-directed RNA polymerase. Reaction: nucleoside triphosphate + RNAn = diphosphate + RNAn+1. Other name(s): RNA polymerase; RNA nucleotidyltransferase (DNA-directed); RNA polymerase I; RNA polymerase II; RNA polymerase III; RNA nucleotidyltransferase (DNA-directed); C RNA formation factors; deoxyribonucleic acid-dependent ribonucleic acid polymerase; DNA-dependent ribonucleate nucleotidyltransferase; DNA-dependent RNA nucleotidyltransferase; DNA-dependent RNA polymerase; ribonucleate nucleotidyltransferase; ribonucleate polymerase; C ribonucleic acid formation factors;ribonucleic acid nucleotidyltransferase; ribonucleic acid polymerase; ribonucleic acid transcriptase; ribonucleic polymerase; ribonucleic transcriptase; C RNA formation factors; RNA nucleotidyltransferase; RNA transcriptase; transcriptase; RNA nucleotidyltransferase I. Systematic name: nucleoside-triphosphate:RNA nucleotidyltransferase (DNA-directed). Comments: Catalyses DNA-template-directed extension of the 3- ...
Apparently, adding a different counterion makes a world of difference. This week, Honeywell reported a new fertilizer, Sulf-N26, which is just a mix of ammonium nitrate and ammonium sulfate. Apparently, it doesnt support the explosive combustion of fuel oil like pure ammonium nitrate does.. ...
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Get an answer for 100 ml of a solution containing dissolved ammonium sulfate and copper (II) sulfate is divided into two equal portions. When excess barium chloride was added to the first portion it was found that 3.5g of barium sulfate was precipitated. When excess sodium carbonate was added to the second portion, all the copper ions were precipitated as copper (II) carbonate. heating of the carbonate gave 0.6360 g of copper(II)oxide. Calculate the mass of each compound in the original mixture. and find
Ammonium sulfate protein precipitation is a key technique for concentration, fractionation and purification of proteins. Salting-out is a key technique.
1. Place a heavy inoculum from an actively growing culture slant on the surface of each of 2 tubes of Pyrazinamide Agar. Use enough inoculum so that it may be readily seen with the naked eye. Do not stab the medium. 2. Incubate two (2) uninoculated tubes and two (2) uninoculated control tubes aerobically at 35-37ºC. 3. After 4 days incubation, remove one (1) uninoculated tube and one (1) control tube from the incubator and add 1ml of freshly prepared 1% ferrous ammonium sulfate solution (in deionized water) to the tubes. 4. Place the tubes at room temperature for 30 minutes, then examine for a pink band in the medium. Refrigerate negative tubes for 4 hours at 2-8ºC. to prevent growth of contaminants. 5. After 4 hours, examine for a pink color development by holding the tube against a white background using incandescent room light. If the 4 day test is positive, it is not necessary to hold the second tube for the 7 day test. 6. The control must be negative for the test to be valid. If the 4 day ...
30% polyethlene glycol monomethyl ether 5000, 0.2M ammonium sulfate, 0.1M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature ...
Chromium (III) potassium sulfate solution. A test tube containing 0.15 M solution of chromium (III) potassium sulfate (KCr(SO4)2) in water. - Stock Image C028/0965
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9. Changshu Xinzhuang Jixiang Auxiliary. Toward the starting, the report covers the top Ammonium Hexaflorozirconate fabricating industry players from areas like United States, EU, Japan, and China. It likewise describes the market in view of geological districts.. Further, the Ammonium Hexaflorozirconate report gives data on the organization profile, a piece of the pie and contact subtle elements alongside esteem chain investigation of Ammonium Hexaflorozirconate industry, Ammonium Hexaflorozirconate industry tenets and arrangements, conditions driving the development of the market and impulse hindering the development. Ammonium Hexaflorozirconate Market improvement scope and different business procedures are additionally specified in this report.. Enquire Here: www.qymarketresearch.com/report/139228#inquiry-for-buying. The Ammonium Hexaflorozirconate look into report incorporates the items that are right now sought after and accessible in the market alongside their cost separation, producing ...
Definition of Phenylmercuric acetate with photos and pictures, translations, sample usage, and additional links for more information.
Crystallization trials were carried out by sitting-drop vapour diffusion with a Rigaku Phoenix automated dispenser and commercially available screens. In addition to the apo form, attempts were made to crystallize binary complexes with NADPH or guanosine monophosphate (GMP) by incubating the protein with the ligands at 5 mM, which is about a 20-fold molar excess, for 1 h prior to setting up trials. Initial hits were optimized using hanging-drop vapour diffusion and the best crystals of the apo protein (form I) were obtained with the reservoir conditions 0.9 M ammonium phosphate, 0.075 M sodium acetate pH 4.5 from drops consisting of 1 µl protein solution and 1 µl reservoir solution. Crystals were obtained from the sample containing GMP (form II) with the reservoir conditions 0.2 M ammonium sulfate, 0.125 M sodium cacodylate pH 5, 30% polyethylene glycol 8000. No crystals were observed in the presence of NADPH. For reasons that are unclear, crystals were only obtained from the sample comprising ...
Studies were undertaken to determine the extent and rate of binding of Hg-203 labeled phenylmercuric acetate and mercuric ace tate in rat kidney and liver slices and their subcellular fractions after 37°C incubation of the slices in Krebs-Ringer -phosphate solutions containing the mercurials at 10⁻⁴ molar. A fast and parallel rate of uptake of both mercurials in kidney slices between 1 to 3 hour periods was observed. The uptake of the two mercurials into liver slices was much less than that found in kidney slices, The binding rate of phenylmercuric acetate was almost double the rate of inorganic mercury. The subcellular fractions (nuclear, mitochondrial, microsomal, and soluble) of the tissue slices were prepared by homogenizing in 0.25 molar sucrose with subsequent differential centrifugation. Even though the two mercurials showed similar binding in kidney slices, it was found that phenylmercuric acetate was bound to almost twice the extent that of inorganic mercury in the mitochondria, ...
The main air pollutants in the sintering process of iron ore are polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans (PCDD/Fs) and harmful dust. Ore sintering on sinter strands is one of the first technology steps in the ironworks. It is a process in which iron ore is crushed, subjected to annealing and mixed with appropriate additives, and then sintered in order to produce sinter which is the main component of iron in the blast furnace process. PCDD/Fs emissions were measured and the addition of ammonium sulfate as an inhibitor of the synthesis of dioxins in the sintering process of iron ore was studied ...
Data & statistics on Ammonium Perchlorate (APC): ชื�อสารเคมีอันตราย (อังกฤษ) AMMONIUM BIFLUORIDE AMMONIUM CHLORIDE AMMONIUM DICHROMATE AMMONIUM FLUORIDE AMMONIUM HYDROGEN DIFLUORIDE AMMONIUM PERCHLORATE AMMONIUM POLYSULPHIDES AMMONIUM SALT OF DNOC AMMONIUM SULFAMATE AMMONIUM BIS(2,4,6-TRINITROPHENY)AMINE AMYL ACETATE, ALL ISOMERS AMYL ALCOHOL,except tert-PENTANOL AMYL FORMATE AMYL PROPIONATE 4-AMINODIPHENYL ANILINE ANILINE(SALTS) o-ANISIDINE p-ANISIDINE ..., Cyclic voltammograms of C60 /Pd films in acetonitrile containing 1 0.10 M tetra n-hexyl ammonium perchlorate, 2 0.10 M tetra n-butyl ammonium perchlorate, 3 0.10 M tetra ethyl ammonium perchlorate, and 4 0.07 M tetra methyl ammonium perchlorate. The sweep rate was 50 mV/s. The C60 /Pd films were grown under cyclic voltammetry, A mixture of Octogen (NMX) and TNT 70/30 and 75/25. Performance values...

SULFURIC ACID (H2SO4)SULFURIC ACID (H2SO4)

Its main use is in phosphate fertilizer production, both superphosphate of lime and ammonium sulfate. It is widely also used to ... vitriol is zinc sulfate. Alums are solid sulfates, containing two different metals and two sulfate radicals. Organic sulfates ... Sulfates are widely distributed in nature. Barium sulfate occurs as barite; calcium sulfate is found as gypsum, alabaster, and ... Some sulfates were formerly known as vitriols; blue vitriol is cupric sulfate, green vitriol is ferrous sulfate, and white ...
more infohttp://chemicalland21.com/industrialchem/inorganic/SULFURIC%20ACID.htm

Ammonium sulfate - WikipediaAmmonium sulfate - Wikipedia

Double metal sulfates include ammonium cobaltous sulfate, ferrous diammonium sulfate, ammonium nickel sulfate which are known ... Ammonium sulfate. Ammonium sulfate (2:1). Diammonium sulfate. Sulfuric acid diammonium salt. Mascagnite. Actamaster. Dolamin ... The filtrate on evaporation yields ammonium chloride. Ammonium sulfate forms many double salts (ammonium metal sulfates) when ... Ammonium sulfate (American English and international scientific usage; ammonium sulphate in British English); (NH4)2SO4, is an ...
more infohttps://en.wikipedia.org/wiki/Ammonium_sulphate

Ammonium sulfateAmmonium sulfate

The National Institute of Standards and Technology (NIST) uses its best efforts to deliver a high quality copy of the Database and to verify that the data contained therein have been selected on the basis of sound scientific judgment. However, NIST makes no warranties to that effect, and NIST shall not be liable for any damage that may result from errors or omissions in the Database ...
more infohttps://webbook.nist.gov/cgi/inchi/InChI%3D1S/H8N2O4S/c1-5-7

ammonium sulfate cutsammonium sulfate cuts

... Ding Ming ming at mbcrr.harvard.edu Mon Apr 10 09:28:52 EST 1995 *Previous message: ammonium sulfate ... You have to watch the temperature while making the ammonium sulfate solution. The best way is to make some over-satuated slurry ...
more infohttp://www.bio.net/bionet/mm/methods/1995-April/027026.html

Ammonium sulfate - New World EncyclopediaAmmonium sulfate - New World Encyclopedia

Ammonium sulfate. Other names. ammonium sulfate (2:1);. diammonium sulfate;. sulfuric acid diammonium salt;. mascagnite;. ... Ammonium sulfate is prepared commercially by reacting ammonia with sulfuric acid (H2SO4). Ammonium sulfate is prepared ... Chemical Profile: Ammonium Sulfate. *Using Ammonium Sulfate Fertilizer as an Organic Mulch Fire Retardant (A Research Note) ... ammonium sulfate precipitation is a common method for purifying proteins by precipitation. As such, ammonium sulfate is also ...
more infohttps://www.newworldencyclopedia.org/entry/Ammonium_sulfate

Boost Fall Tillering with Ammonium SulfateBoost Fall Tillering with Ammonium Sulfate

Ammonium nitrogen the form in ammonium sulfate also improved root growth and reduced damage from take-all in research at Purdue ... Adding ammonium sulfate to blends or solutions with urea or urea ammonium nitrate (UAN) contributes both nitrogen and sulfur in ... Visit www.sulfn.com or contact your Honeywell representative for more information on how Sulf-N ® ammonium sulfate can boost ... its a great time to consider the role of a pre-plant application of ammonium sulfate in improving wheat yields.Wheat demands a ...
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Iron (II) Ammonium Sulfate | Carolina.comIron (II) Ammonium Sulfate | Carolina.com

Ferrous ammonium sulfate Formula: Fe(NH4)2(SO4)2 • 6H2O Formula Wt.: 392.14 CAS No.: 7783-85-9 Characteristics: Pale blue-green ... Synonym: Ferrous ammonium sulfate. Formula: Fe(NH4)2(SO4)2 • 6H2O. Formula Wt.: 392.14. CAS No.: 7783-85-9. Characteristics: ... Synonym: Ferrous ammonium sulfate. Formula: Fe(NH4)2(SO4)2 • 6H2O. Formula Wt.: 392.14. CAS No.: 7783-85-9. Characteristics: ... Iron (II) Ammonium Sulfate. 2 Items Online Only. *bvseo_sdk, java_sdk, bvseo-4.0.0 ...
more infohttps://www.carolina.com/specialty-chemicals-d-l/iron-ii-ammonium-sulfate/FAM_862872.pr

Preliminary Conference -- Ammonium Sulfate from China | USITCPreliminary Conference -- Ammonium Sulfate from China | USITC

Home » Preliminary Conference -- Ammonium Sulfate from China. Preliminary Conference -- Ammonium Sulfate from China. Date: ... Preliminary phase antidumping and countervailing duty investigations: Ammonium Sulfate from China, Inv. Nos. 701-TA-562 and 731 ...
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Ammonium Sulphate ppt + His TagsAmmonium Sulphate ppt + His Tags

... Jared Head bijgh at zeus.bris.ac.uk Thu Apr 24 09:24:36 EST 1997 *Previous message: ... Furhter to the sensible sounding advice of using a simple Ammonium Sulphate precipiation in conjunction with a nickel column ... Whats the easiest and most systematic way of determining what percentages of Ammonium Sulphate are needed for a particular ...
more infohttp://www.bio.net/bionet/mm/methods/1997-April/056912.html

Ammonium sulphate - Substance Information - ECHAAmmonium sulphate - Substance Information - ECHA

... multi constituent substance of ammonium dihydrogenorthophosphate and ammonium nitrate and ammonium sulphate and potassium ... Ammonium sulphate. ↓Other names: Regulatory process names [3] Trade names [67] IUPAC names [28] ... Ammonium sulphate nitrat (ASN) * Ammonium sulphate solution * Ammonium Sulphate Standard * Ammonium Sulphate standard without ... 231-984-1, Koksownia Częstochowa Nowa, ammonium sulphate * 231-984-1, Kombinat Koksochemiczny Zabrze S.A., ammonium sulphate ...
more infohttps://echa.europa.eu/de/substance-information/-/substanceinfo/100.029.076

Tables for the preparation of ammonium sulfate solutions.  - PubMed - NCBITables for the preparation of ammonium sulfate solutions. - PubMed - NCBI

PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
more infohttps://www.ncbi.nlm.nih.gov/pubmed/942105?dopt=Abstract

Ammonium sulfate - definition of ammonium sulfate by The Free DictionaryAmmonium sulfate - definition of ammonium sulfate by The Free Dictionary

ammonium sulfate synonyms, ammonium sulfate pronunciation, ammonium sulfate translation, English dictionary definition of ... ammonium sulfate. n. An odorless white crystalline salt, 2SO4, used in fertilizers, fire retardants, and water purification. n ... Ammonium sulfate - definition of ammonium sulfate by The Free Dictionary https://www.thefreedictionary.com/ammonium+sulfate ... ammonium sulfate. Also found in: Thesaurus, Medical, Acronyms, Encyclopedia, Wikipedia. ammonium sulfate. n.. An odorless white ...
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Ammonium sulfate precipitaion problem - Molecular Biology - BioForumAmmonium sulfate precipitaion problem - Molecular Biology - BioForum

I cannot fractionate transgene protein from plant protein by ammonium sulfate precipitation. The total protein is extracted ... Ammonium sulfate precipitaion problem - posted in Molecular Biology: Hi, ... So I think ammonium sulfate can help me to get rid of the other proteins. After precipitation with 20 to 90 % saturation, about ... Ammonium sulfate precipitaion problem. Started by Jack, Apr 15 2002 10:20 AM ...
more infohttp://www.protocol-online.org/forums/topic/357-ammonium-sulfate-precipitaion-problem/

Ammonium Sulfate, Crystal, Reagent, ACS, 99%, Spectrum
 | Fisher ScientificAmmonium Sulfate, Crystal, Reagent, ACS, 99%, Spectrum | Fisher Scientific

Spectrum Ammonium Sulfate, Crystal, Reagent, ACS, 99%, Spectrum™, 125 g, Plastic Bottle, Plastic Bottle Chemicals:Other ... Spectrum™ Ammonium Sulfate, Crystal, Reagent, ACS is an inorganic salt that is very soluble in water Most often used as a soil ...
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High Purity Ammonium Sulphate Wholesale, Ammonium Sulphate Suppliers - AlibabaHigh Purity Ammonium Sulphate Wholesale, Ammonium Sulphate Suppliers - Alibaba

High Purity Ammonium Sulphate Products from Global High Purity Ammonium Sulphate Suppliers and High Purity Ammonium Sulphate ... High Purity Ammonium Sulphate, Wholesale Various High Quality ... ammonium sulphate ammonium sulfate nickel ammonium sulphate ... granular ammonium sulphate ammonium phosphate sulphate preparation of ammonium sulphate liquid ammonium sulphate ammonium ... n21 sinopec ammonium sulphate 21% crystal ammonium sulphate supply 21%n ammonium sulphate ammonium sulphate in chemical More... ...
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Ferric Ammonium Sulfate Dodecahydrate, Reagent Grade, 100 g | Carolina.comFerric Ammonium Sulfate Dodecahydrate, Reagent Grade, 100 g | Carolina.com

Ferric ammonium sulfate, dodecahydrate Formula: FeNH4(SO4)2•12H2O F.W.: 482.19 Characteristics: Pale violet crystals Storage ... Synonym: Ferric ammonium sulfate, dodecahydrate Formula: FeNH4(SO4)2•12H2O. F.W.: 482.19. Characteristics: Pale violet crystals ... Synonym: Ferric ammonium sulfate, dodecahydrate Formula: FeNH4(SO4)2¥12H2O. F.W.: 482.19. Characteristics: Pale violet crystals ... Ferric Ammonium Sulfate Dodecahydrate, Reagent Grade, 100 g. Item # 862734 *bvseo_sdk, java_sdk, bvseo-4.0.0 ...
more infohttps://www.carolina.com/specialty-chemicals-d-l/ferric-ammonium-sulfate-dodecahydrate-reagent-grade-100-g/862734.pr

Ammonium sulfate, 99%, extra pure, ACROS Organics™Ammonium sulfate, 99%, extra pure, ACROS Organics™

ammonium sulfate, diammonium sulfate, ammonium sulphate, mascagnite, sulfuric acid diammonium salt, ammonium sulfate 2:1, ... ammonium sulfate, diammonium sulfate, ammonium sulphate, mascagnite, sulfuric acid diammonium salt, ammonium sulfate 2:1, ...
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Aluminum Ammonium Sulfate pharmaceutical drugs and health productsAluminum Ammonium Sulfate pharmaceutical drugs and health products

Aluminum Ammonium Sulfate indications and usages ATC and ICD codes, combinations with other active ingredients and trade names ... Aluminum Ammonium Sulfate Alum - Ratiopharm Aluminum Ammonium Sulfate Dodecahydrate Alum - Produits Marc-O 1987. Alum - Regal ... Ferrous Sulfate. Tannic Acid Blood Stopper - Equivet; Remedy Animal Health Products Aluminum Ammonium Sulfate Dodecahydrate. ... Aluminum Ammonium Sulfate Dodecahydrate. Copper (Copper Sulfate). Iodophor. Iron (Ferrous Subsulfate). Kaolin Gimborn Quik Klot ...
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Aluminum Ammonium Sulfate 12-Hydrate | Boreal ScienceAluminum Ammonium Sulfate 12-Hydrate | Boreal Science

Synonyms: Ammonium Alum, Aluminum Ammonium Sulfate Dodecahydrate. Shelf Life (months): 36. Storage: Green. ... Aluminum Ammonium Sulfate 12-Hydrate Reagent. 500 g. Crystal. Each (500g). Retrieving. ...
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Ammonium Sulfate Market: By Application & Geography - Forecast 2016-2021Ammonium Sulfate Market: By Application & Geography - Forecast 2016-2021

Ammonium sulfate has a high sulfur content in the sulfate form which occurs naturally in volcanic fumaroles as a rare mineral, ... Ammonium Sulfate Market. Globally Asia-Pacific dominated market for ammonium sulfate in 2015 and is estimated to continue the ... Asia-Pacific ammonium sulfate market is expected be followed by North America and European ammonium sulphate market ... Ammonium sulfate has a high sulfur content in the sulfate form which occurs naturally in volcanic fumaroles as a rare mineral, ...
more infohttps://www.researchandmarkets.com/reports/3820799/ammonium-sulfate-market-by-application-and

Ammonium sulfate sales volume UK 2011-2017 | StatistaAmmonium sulfate sales volume UK 2011-2017 | Statista

This statistic shows the sales volume of ammonium sulfate based on PRODCOM estimates** in the United Kingdom between 2011 and ... Ammonium sulfate: sales volume in the United Kingdom (UK) 2011-2017. Sales volume of ammonium sulfate in the United Kingdom (UK ... Ammonium sulphate excluding in tablets or similar forms or in packages of a weight of 10kg or less. ... Ammonium sulphate excluding in tablets or similar forms or in packages of a weight of 10kg or less. ...
more infohttps://www.statista.com/statistics/346314/ammonium-sulfate-sales-volume-in-the-united-kingdom-uk/

Ammonium Sulphate - Qingzhou Pengbo Chemical Co.,Ltd - ecplaza.netAmmonium Sulphate - Qingzhou Pengbo Chemical Co.,Ltd - ecplaza.net

Ammonium Sulphate CAS No.: 7783-20-2 Other Names: ammonium sulfate MF: (NH4)2SO4 Place of Origin:China (Mainland) Release Type ...
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3.1 M Ammonium Sulfate Solution | LeeBio.com3.1 M Ammonium Sulfate Solution | LeeBio.com

3.1 M ammonium sulfate solution with 20 mM tris chloride, 1 mM DTT and 1 mM EDTA, pH 7.5.. ...
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N 21% Ammonium Sulphate Manufacturer, Supplier & Exporter - ecplaza.netN 21% Ammonium Sulphate Manufacturer, Supplier & Exporter - ecplaza.net

Item Steel grade ammonium sulphate Nitrogen 20.5 min Sulfur 23min Moisture 1.5max Free acidity 0.2max Standard GB535-1995 MF ( ...
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Global Dihexadecyl Dimethyl Ammonium Sulfate Market Research Report 2017Global Dihexadecyl Dimethyl Ammonium Sulfate Market Research Report 2017

Dihexadecyl Dimethyl Ammonium Sulfate Report by Material, Application, - Market research report and industry analysis - ... Global Dihexadecyl Dimethyl Ammonium Sulfate Market Research Report 2017 Global Dihexadecyl Dimethyl Ammonium Sulfate Market ... Part I Dihexadecyl Dimethyl Ammonium Sulfate Industry Overview. Chapter One Dihexadecyl Dimethyl Ammonium Sulfate Industry ... Global Dihexadecyl Dimethyl Ammonium Sulfate Market Research Report 2017. Dihexadecyl Dimethyl Ammonium Sulfate Report by ...
more infohttps://www.marketresearch.com/9Dimen-Research-v4004/Global-Dihexadecyl-Dimethyl-Ammonium-Sulfate-10705225/
  • Ammonium nitrogen resists loss from volatilization roughly five to ten times less volatilization loss than urea according to research at the University of Arkansas. (honeywell.com)
  • ChEBI: A hydrate that is the hexahydrate form of ferrous ammonium sulfate. (chemicalbook.com)
  • Ferrous ammonium sulfate hexahydrate is used in photography, analytical chemistry and in dosimeters. (chemicalbook.com)
  • Ferrous ammonium sulfate hexahydrate Anbieter Lieferant Produzent Hersteller Vertrieb Händler. (chemicalbook.com)
  • Ammonium sulfate is especially useful as a precipitant because it is highly soluble, stabilizes protein structure, has a relatively low density, is readily available, and is relatively inexpensive. (wikipedia.org)
  • As such, ammonium sulfate is also listed as an ingredient in many vaccines used in the United States , according to the Centers for Disease Control and Prevention (CDC). (newworldencyclopedia.org)
  • Ammonium sulfate is listed as an ingredient for many United States vaccines per the Center for Disease Control. (wikipedia.org)
  • Ammonium phosphate and ammonium sulfate , known for their use as agricultural fertilizers, are also often added to provide nutrients to help the forest regenerate after a burn. (thefreedictionary.com)
  • Note that for an ammonium sulfate suspension, most of the enzyme will be in solid form. (sigmaaldrich.com)
  • It is likely that only negligible amounts of enzyme will be in the ammonium sulfate solution. (sigmaaldrich.com)
  • Agricultural Use Ammonium Sulfate is used primarily where there is. (spintoband.com)
  • Due to the high dielectric constant of water, the dissociated salt ions being cationic ammonium and anionic sulfate are readily solvated within hydration shells of water molecules. (wikipedia.org)
  • Dear Sulf-N Ambassador:As farmers tune up their drills for wheat planting, it's a great time to consider the role of a pre-plant application of ammonium sulfate in improving wheat yields.Wheat demands a lot of sulfur an 80-bushel wheat crop needs 20 pounds of sulfur per acre, and a 100-bushel crop will utilize 25 pounds of sulfur per season. (honeywell.com)
  • Research at North Carolina State University recorded 13% higher winter wheat yields as a result of better fall tillering following ammonium sulfate applications. (honeywell.com)
  • after extraction u should first precipitate with Amonium sulphate(with required %) and then dialyse in 1:10 times of buffer. (protocol-online.org)
  • Furhter to the sensible sounding advice of using a simple Ammonium Sulphate precipiation in conjunction with a nickel column His.Tag purification, can anybody give me advice on how to set yourself up for such a step. (bio.net)
  • Therefore, the present study aimed to evaluate the interactions of sulphur sources via ammonium sulfate and calcium sulfate on the population dynamics of S. (thefreedictionary.com)