Ammonia
Lyases
Chondroitin Lyases
Enzymes which catalyze the elimination of delta-4,5-D-glucuronate residues from polysaccharides containing 1,4-beta-hexosaminyl and 1,3-beta-D-glucuronosyl or 1,3-alpha-L-iduronosyl linkages thereby bringing about depolymerization. EC 4.2.2.4 acts on chondroitin sulfate A and C as well as on dermatan sulfate and slowly on hyaluronate. EC 4.2.2.5 acts on chondroitin sulfate A and C.
Pectobacterium chrysanthemi
Chondroitinases and Chondroitin Lyases
Pectins
High molecular weight polysaccharides present in the cell walls of all plants. Pectins cement cell walls together. They are used as emulsifiers and stabilizers in the food industry. They have been tried for a variety of therapeutic uses including as antidiarrheals, where they are now generally considered ineffective, and in the treatment of hypercholesterolemia.
Chicory
Aldehyde-Lyases
Nitrosomonas
Carbon-Oxygen Lyases
Erwinia
Heparin Lyase
An enzyme of the isomerase class that catalyzes the eliminative cleavage of polysaccharides containing 1,4-linked D-glucuronate or L-iduronate residues and 1,4-alpha-linked 2-sulfoamino-2-deoxy-6-sulfo-D-glucose residues to give oligosaccharides with terminal 4-deoxy-alpha-D-gluc-4-enuronosyl groups at their non-reducing ends. (From Enzyme Nomenclature, 1992) EC 4.2.2.7.
Carbamoyl-Phosphate Synthase (Ammonia)
Oxo-Acid-Lyases
Nitrogen
Polygalacturonase
Hyperammonemia
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Urea
Glutamate-Ammonia Ligase
Alginates
Glutamine
Hydrogen-Ion Concentration
Substrate Specificity
Ammonium Chloride
Nitrogen Radioisotopes
Hexuronic Acids
Isocitrate Lyase
Nitrification
Rhodophyta
Plants of the division Rhodophyta, commonly known as red algae, in which the red pigment (PHYCOERYTHRIN) predominates. However, if this pigment is destroyed, the algae can appear purple, brown, green, or yellow. Two important substances found in the cell walls of red algae are AGAR and CARRAGEENAN. Some rhodophyta are notable SEAWEED (macroalgae).
Phycobilins
Chondroitin ABC Lyase
Sphingomonas
Amino Acid Sequence
Cytochromes c1
Bacteroides
Glucuronic Acid
Flavobacterium
Adenylosuccinate Lyase
Streptococcus anginosus
Cloning, Molecular
Hevea
Hepatic Encephalopathy
A syndrome characterized by central nervous system dysfunction in association with LIVER FAILURE, including portal-systemic shunts. Clinical features include lethargy and CONFUSION (frequently progressing to COMA); ASTERIXIS; NYSTAGMUS, PATHOLOGIC; brisk oculovestibular reflexes; decorticate and decerebrate posturing; MUSCLE SPASTICITY; and bilateral extensor plantar reflexes (see REFLEX, BABINSKI). ELECTROENCEPHALOGRAPHY may demonstrate triphasic waves. (From Adams et al., Principles of Neurology, 6th ed, pp1117-20; Plum & Posner, Diagnosis of Stupor and Coma, 3rd ed, p222-5)
Sequence Homology, Amino Acid
Manure
Nitrosomonas europaea
Carbon-Nitrogen Lyases
Glutamate Dehydrogenase
Carbon-Carbon Lyases
Glycosaminoglycans
Proteus vulgaris
Uronic Acids
Rumen
The first stomach of ruminants. It lies on the left side of the body, occupying the whole of the left side of the abdomen and even stretching across the median plane of the body to the right side. It is capacious, divided into an upper and a lower sac, each of which has a blind sac at its posterior extremity. The rumen is lined by mucous membrane containing no digestive glands, but mucus-secreting glands are present in large numbers. Coarse, partially chewed food is stored and churned in the rumen until the animal finds circumstances convenient for rumination. When this occurs, little balls of food are regurgitated through the esophagus into the mouth, and are subjected to a second more thorough mastication, swallowed, and passed on into other parts of the compound stomach. (From Black's Veterinary Dictionary, 17th ed)
Pseudomonas
Chondroitin Sulfates
Derivatives of chondroitin which have a sulfate moiety esterified to the galactosamine moiety of chondroitin. Chondroitin sulfate A, or chondroitin 4-sulfate, and chondroitin sulfate C, or chondroitin 6-sulfate, have the sulfate esterified in the 4- and 6-positions, respectively. Chondroitin sulfate B (beta heparin; DERMATAN SULFATE) is a misnomer and this compound is not a true chondroitin sulfate.
Amino Acids
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Sulfonium Compounds
Phenylalanine Ammonia-Lyase
Gills
Dermatan Sulfate
Crystallography, X-Ray
Sequence Alignment
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Carbohydrate Sequence
Glutamate Synthase
DNA-(Apurinic or Apyrimidinic Site) Lyase
A DNA repair enzyme that catalyses the excision of ribose residues at apurinic and apyrimidinic DNA sites that can result from the action of DNA GLYCOSYLASES. The enzyme catalyzes a beta-elimination reaction in which the C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. This enzyme was previously listed under EC 3.1.25.2.
Quaternary Ammonium Compounds
Glutamates
Bacillus
Archaea
One of the three domains of life (the others being BACTERIA and Eukarya), formerly called Archaebacteria under the taxon Bacteria, but now considered separate and distinct. They are characterized by: (1) the presence of characteristic tRNAs and ribosomal RNAs; (2) the absence of peptidoglycan cell walls; (3) the presence of ether-linked lipids built from branched-chain subunits; and (4) their occurrence in unusual habitats. While archaea resemble bacteria in morphology and genomic organization, they resemble eukarya in their method of genomic replication. The domain contains at least four kingdoms: CRENARCHAEOTA; EURYARCHAEOTA; NANOARCHAEOTA; and KORARCHAEOTA.
Sequence Analysis, DNA
N-Glycosyl Hydrolases
Urease
Fatty Acids, Volatile
Catalysis
Fungi
A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies.
Base Sequence
Electrophoresis, Polyacrylamide Gel
Acidosis
Biocatalysis
Nitrogen Isotopes
Catalytic Domain
Models, Molecular
Chromatography, Gel
DNA Glycosylases
A family of DNA repair enzymes that recognize damaged nucleotide bases and remove them by hydrolyzing the N-glycosidic bond that attaches them to the sugar backbone of the DNA molecule. The process called BASE EXCISION REPAIR can be completed by a DNA-(APURINIC OR APYRIMIDINIC SITE) LYASE which excises the remaining RIBOSE sugar from the DNA.
Temperature
Urea Cycle Disorders, Inborn
Rare congenital metabolism disorders of the urea cycle. The disorders are due to mutations that result in complete (neonatal onset) or partial (childhood or adult onset) inactivity of an enzyme, involved in the urea cycle. Neonatal onset results in clinical features that include irritability, vomiting, lethargy, seizures, NEONATAL HYPOTONIA; RESPIRATORY ALKALOSIS; HYPERAMMONEMIA; coma, and death. Survivors of the neonatal onset and childhood/adult onset disorders share common risks for ENCEPHALOPATHIES, METABOLIC, INBORN; and RESPIRATORY ALKALOSIS due to HYPERAMMONEMIA.
Oxidation-Reduction
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
Intramolecular Lyases
Air
Oxidoreductases
The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)
Enzyme Stability
Bacteria
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
Heparitin Sulfate
Isoenzymes
Chromatography
Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.
Oncorhynchus mykiss
A large stout-bodied, sometimes anadromous, TROUT found in still and flowing waters of the Pacific coast from southern California to Alaska. It has a greenish back, a whitish belly, and pink, red, or lavender stripes on the sides, with usually a sprinkling of black dots. It is highly regarded as a sport and food fish. Its former name was Salmo gairdneri. The sea-run rainbow trouts are often called steelheads. Redband trouts refer to interior populations of rainbows.
Persea
Ketoglutaric Acids
Gene Expression Regulation, Bacterial
The crystal structure of the formiminotransferase domain of formiminotransferase-cyclodeaminase: implications for substrate channeling in a bifunctional enzyme. (1/224)
BACKGROUND: The bifunctional enzyme formiminotransferase-cyclodeaminase (FTCD) contains two active sites at different positions on the protein structure. The enzyme binds a gamma-linked polyglutamylated form of the tetrahydrofolate substrate and channels the product of the transferase reaction from the transferase active site to the cyclodeaminase active site. Structural studies of this bifunctional enzyme and its monofunctional domains will provide insight into the mechanism of substrate channeling and the two catalytic reactions. RESULTS: The crystal structure of the formiminotransferase (FT) domain of FTCD has been determined in the presence of a product analog, folinic acid. The overall structure shows that the FT domain comprises two subdomains that adopt a novel alpha/beta fold. Inspection of the folinic acid binding site reveals an electrostatic tunnel traversing the width of the molecule. The distribution of charged residues in the tunnel provides insight into the possible mode of substrate binding and channeling. The electron density reveals that the non-natural stereoisomer, (6R)-folinic acid, binds to the protein; this observation suggests a mechanism for product release. In addition, a single molecule of glycerol is bound to the enzyme and indicates a putative binding site for formiminoglutamate. CONCLUSIONS: The structure of the FT domain in the presence of folinic acid reveals a possible novel mechanism for substrate channeling. The position of the folinic acid and a bound glycerol molecule near to the sidechain of His82 suggests that this residue may act as the catalytic base required for the formiminotransferase mechanism. (+info)Mechanism of action of ethanolamine ammonia-lyase, an adenosylcobalamin-dependent enzyme. Proton nuclear magnetic resonance studies of the binding of adenine nucleosides and substrate to ethanolamine ammonia-lyase. (2/224)
Proton NMR spectroscopy was used to study the binding of adenosine, 5'-deoxyadenosine, adenine, and ethanolamine to the adenosylcobalamin-dependent enzyme ethanolamine ammonia-lyase. Broadening of ligand resonances in the presence of ethanolamine ammonia-lyase indicated that adenosine, 5'-deoxyadenosine, and ethanolamine all formed complexes with the enzyme (KD(mM) = 3.5, 3.0, and 2.5 respectively). The methyl group of enzyme-bound 5'-deoxyadenosine rotated at a rate exceeding 10(7) revolutions/s. Adenine did not appear to bind to the enzyme. Rates of dissociation of nucleosides from the enzyme were fast on the NMR time scale, precluding measurements of rate constants for the binding reaction. The departure of ethanolamine was slow, however, permitting their determination. The values for these rate constants were: k1 = 4.4 times 10(5) M-1 S-1; k-1 = 1.1 times 10(3) S-1. Addition of 1 mol of cyanocobalamin/mol of active sites led to narrowing of the enzyme-broadened ligand resonances. With 5'-deoxyadenosine, linewidths still exceeded those of the free ligand, indicating that binding to enzyme was weakened but not abolished. The KD for this nucleoside in the presence of CNCbl was 8.0 mM. With ethanolamine and adenosine, however, linewidths reverted to values characteristic of the unbound ligand, indicating either that CNCbl greatly lowered the rate of dissociation of the ligand or displaced the ligand from the enzyme. A decision between these two possibilities could not be made from the data at hand, although analogy with the situation obtaining with 5'-deoxyadenosine suggests that adenosine is displaced from the enzyme by CNCbl. 5'-Deoxyadenosine inhibited catalytic activity of the enzyme, competing with adenosylcobalamin (Ki = 2.7 mM). Adenosine had no effect, despite NMR evidence indicating that it formed a complex with free enzyme. These experiments showed that ethanolamine ammonia-lyase possesses binding sites for adenine nucleosides, a class of compounds chemically related to the Cobeta-ligand of the cofactor, as well as for ethanolamine. Binding to the enzyme has now been demonstrated for all three categories of low molecular weight compounds thought to be involved in the reaction; namely, substrate (ethanolamine), corrin, and adenine nucleoside. (+info)Interaction between ethanolamine ammonia-lyase and methylcobalamin. Half-site reactivity with an adenosylcobalamin-dependent enzyme. (3/224)
The adenosylcobalamin-dependent enzyme ethanolamine ammonia-lyase contains two active sites per molecule. The effects of methylcobalamin on the properties of this enzyme differ qualitatively depending on whether one or both of these sites is occupied by the cobamide. At 0.5 mol of methylcobalamin/mol of active sites, catalytic activity fell rapidly to approximately 30% of control levels, thereafter remaining constant for an hour. With the partially inhibited enzyme, Km values for ethanolamine and adenosylcobalamin were 5.5 muM and 1.6 muM, respectively, values that do not differ significantly from those of uninhibited enzyme. When the methylcobalamin per active site ratio was increased to 1, the decline in activity became progressive with time, eventually falling to levels much lower than seen at a cobamide per active site ratio of 0.5. Methylcobalamin also promotes the formation of a complex stable to gel filtration between ethanolamine and enzyme. Complex formation increased with increasing methylcobalamin per active site ratios up to a ratio of 0.7/1, at which point 0.5 mol of ethanol/mol of active sites was taken up. Ethanolamine uptake did not increase at higher methylcobalamin to active sites ratios. Methylcobalamin itself was taken up by enzyme, forming a complex containing 0.5 mol of methylcobalamin/mol of active sites that was stable to gel filtration. Measurement by the technique of Hummel and Dreyer ((1962) Biochim. Biophys. Acta 63, 530-532), however, showed one methylcobalamin binding site per active site. The formation of enzyme-ligand complexes stable to gel filtration was not affected by 5'-deoxyadenosine nor did 5'-deoxyadenosine by itself promote the formation of a stable complex between enzyme and ethanolamine. These observations were interpreted as evidence indicating half-site reactivity of ethanolamine ammonia-lyase with methylcobalamin. Comparison with previous results suggested that this half-site reactivity was an epiphenomenon not related to catalysis. (+info)The mechanism of action of ethanolamine ammonia-lyase, an adenosylcobalamin-dependent enzyme. The source of the third methyl hydrogen in the 5'-deoxyadenosine generated from the cofactor during catalysis. (4/224)
Ethanolamine ammonia-lyase is an adenosylcobalamin-dependent enzyme which catalyzes the conversion of ethanolamine and propanolamine to ammonia and the corresponding aldehydes. A mechanism has been proposed for this and other adenosylcobalamin-dependent reactions which involves cleavage of the carbon-cobalt bond of the cofactor followed by abstraction of a substrate hydrogen atom by the adenosyl fragment to form 5'-deoxyadenosine. In support of this proposal, a previous study demonstrated that the deamination of propanolamine by ethanolamine ammonia-lyase is accompanied by the reversible cleavage of the carbon-cobalt bond of the cofactor, with the production of 5'-deoxyadenosine (Babior, B.M., Carty, T.J., and Abeles, R.H. (1974) J. Biol. Chem. 249, 1689-1695). The present study is concerned with the origin of the third hydrogen atom on the methyl group of the 5'-deoxyadenosine produced in that reaction. The 5'-deoxyadenosine isolated from an incubation mixture initially containing enzyme, [5',5'-D2]adenosylcobalamin, and [1,1-D2]propanolamine was chemically degraded so that the 4' and 5' carbon atoms were, respectively, converted to the carbonyl and methyl carbons of acetaldehyde. Analysis of the p-nitrophenylhydrazone of the acetaldehyde by gas-liquid chromatography-mass spectroscopy revealed 3 deuterium atoms/molecule, indicating that two of the methyl hydrogens originated from adenosylcobalamin and the third was donated by substrate. This observation provides further support for the participation of 5'-deoxyadenosine in the mechanism of adenosylcobalamin-dependent reactions. (+info)Cloning and characterization of the pnb genes, encoding enzymes for 4-nitrobenzoate catabolism in Pseudomonas putida TW3. (5/224)
Pseudomonas putida strain TW3 is able to metabolize 4-nitrotoluene via 4-nitrobenzoate (4NBen) and 3, 4-dihydroxybenzoic acid (protocatechuate [PCA]) to central metabolites. We have cloned, sequenced, and characterized a 6-kbp fragment of TW3 DNA which contains five genes, two of which encode the enzymes involved in the catabolism of 4NBen to PCA. In order, they encode a 4NBen reductase (PnbA) which is responsible for catalyzing the direct reduction of 4NBen to 4-hydroxylaminobenzoate with the oxidation of 2 mol of NADH per mol of 4NBen, a reductase-like enzyme (Orf1) which appears to have no function in the pathway, a regulator protein (PnbR) of the LysR family, a 4-hydroxylaminobenzoate lyase (PnbB) which catalyzes the conversion of 4-hydroxylaminobenzoate to PCA and ammonium, and a second lyase-like enzyme (Orf2) which is closely associated with pnbB but appears to have no function in the pathway. The central pnbR gene is transcribed in the opposite direction to the other four genes. These genes complete the characterization of the whole pathway of 4-nitrotoluene catabolism to the ring cleavage substrate PCA in P. putida strain TW3. (+info)A novel interaction of the Golgi complex with the vimentin intermediate filament cytoskeleton. (6/224)
The integration of the vimentin intermediate filament (IF) cytoskeleton and cellular organelles in vivo is an incompletely understood process, and the identities of proteins participating in such events are largely unknown. Here, we show that the Golgi complex interacts with the vimentin IF cytoskeleton, and that the Golgi protein formiminotransferase cyclodeaminase (FTCD) participates in this interaction. We show that the peripherally associated Golgi protein FTCD binds directly to vimentin subunits and to polymerized vimentin filaments in vivo and in vitro. Expression of FTCD in cultured cells results in the formation of extensive FTCD-containing fibers originating from the Golgi region, and is paralleled by a dramatic rearrangements of the vimentin IF cytoskeleton in a coordinate process in which vimentin filaments and FTCD integrate into chimeric fibers. Formation of the FTCD fibers is obligatorily coupled to vimentin assembly and does not occur in vim(-/-) cells. The FTCD-mediated regulation of vimentin IF is not a secondary effect of changes in the microtubule or the actin cytoskeletons, since those cytoskeletal systems appear unaffected by FTCD expression. The assembly of the FTCD/vimentin fibers causes a coordinate change in the structure of the Golgi complex and results in Golgi fragmentation into individual elements that are tethered to the FTCD/vimentin fibers. The observed interaction of Golgi elements with vimentin filaments and the ability of FTCD to specifically interacts with both Golgi membrane and vimentin filaments and promote their association suggest that FTCD might be a candidate protein integrating the Golgi compartment with the IF cytoskeleton. (+info)Quantitative measurement of the course of bean callus differentiation. (7/224)
Two strains of callus have been isolated from bean hypocotyl and grown on a defined maintenance medium supplemented with 2 mg/l. 2:4-dichlorophenoxyacetic acid (2:4D) and 2% sucrose. Root initiation was observed in one strain and formation of nodules containing xylem and phloem in both strains after transfer to an induction medium supplemented with 1 mg/l. naphthyleneacetic acid, 0-2 mg/l. kinetin and 3% sucrose, after 3 transfers to maintenance medium. The number of nodules per gramme increased 10-fold between 6 and 12 days after transfer, and thereafter remained constant. Phenylalanine ammonia lyase (PAL) activity rose to a maximum value when the rate of nodule formation was greatest, and decreased after the maximum nodule concentration was reached. The final constant value for PAL activity was above that of callus grown on maintenance medium. Beta I leads to 3 glucan synthetase activity rose to a maximum 15 days after transfer, and then fell gradually to a level above that measured in callus on maintenance medium. Callus was transferred from maintenance medium after 3, 4, 5 and 6 transfers. The concentration of nodules after 21 days on induction medium decreased as the callus was kept in culture. No further differentiation could be induced after 6 transfers. The fall in nodule formation was paralleled by a decrease in PAL and betaI leads to 3 glucan synthetase activities measured 21 days after transfer. (+info)Methanococcus jannaschii generates L-proline by cyclization of L-ornithine. (8/224)
Cell extracts of Methanococcus jannaschii have been shown to readily convert L-ornithine to L-proline. This cyclization reaction proceeds with the loss of only the C-2 nitrogen, as has been documented for ornithine cyclodeaminase (EC 4.3.1.12). Since no gene homologous to that coding for ornithine cyclodeaminase is present in the genome of M. jannaschii, these results indicate that proline biosynthesis in M. jannaschii is accomplished by a previously unrecognized enzyme. (+info)
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... was long thought to be an important electrophilic catalytic residue in histidine ammonia-lyase and phenylalanine ammonia-lyase ... "Crystal structure of phenylalanine ammonia lyase: multiple helix dipoles implicated in catalysis". Biochemistry. 43 (36): 11403 ...
Histidine
The enzyme histidine ammonia-lyase converts histidine into ammonia and urocanic acid. A deficiency in this enzyme is present in ... Overall, these reactions result in the formation of glutamate and ammonia. Glutamate can then be deaminated by glutamate ...
Cinnamaldehyde
Kong, Jian-Qiang (2015-07-20). "Phenylalanine ammonia-lyase, a key component used for phenylpropanoids production by metabolic ... of cinnamaldehyde begins with deamination of L-phenylalanine into cinnamic acid by the action of phenylalanine ammonia lyase ( ...
Amino acid
Aspartic acid is produced by the addition of ammonia to fumarate using a lyase. In plants, nitrogen is first assimilated into ... For example, serine dehydratase converts serine to pyruvate and ammonia. After removal of one or more amino groups, the ... However, amino acid degradation can produce uric acid or ammonia instead. ... organic compounds in the form of glutamate, formed from alpha-ketoglutarate and ammonia in the mitochondrion. For other amino ...
Cinnamic acid
Its biosynthesis involves the action of the enzyme phenylalanine ammonia-lyase (PAL) on phenylalanine. It is obtained from oil ...
Amino acid synthesis
Aspartic acid is produced by the addition of ammonia to fumarate using a lyase. Annigan, Jan. "How Many Amino Acids Does the ... In rich nitrogenous media or growth conditions containing high quantities of ammonia there is a low level of GS, whereas in ... This enzyme requires either glutamine as the amino group donor or ammonia itself. Anthranilate synthase is regulated by the ... Cystathionine-β-lyase (in mammals, this step is performed by homocysteine methyltransferase or betaine-homocysteine S- ...
Aspartame
Food portal Medicine portal Aspartame controversy Phenylalanine ammonia lyase Stevia Budavari S, ed. (1989). "861. Aspartame". ...
Tyrosine
The second utilizes enzymatic synthesis from phenolics, pyruvate, and ammonia through the use of tyrosine phenol-lyase. ... Tyrosine ammonia lyase (TAL) is an enzyme in the natural phenols biosynthesis pathway. It transforms L-tyrosine into p-coumaric ...
Eugenol
L-tyrosine is converted to p-coumaric acid by the enzyme tyrosine ammonia lyase (TAL). From here, p-coumaric acid is converted ...
Phenylalanine
... is converted to cinnamic acid by the enzyme phenylalanine ammonia-lyase. The genetic disorder phenylketonuria ( ... In 1882, Erlenmeyer and Lipp first synthesized phenylalanine from phenylacetaldehyde, hydrogen cyanide, and ammonia. The ...
Harvey Levy (academic)
Phenylalanine Ammonia Lyase (PAL): From Discovery to Enzyme Substitution Therapy for Phenylketonuria. Molecular Genetics and ...
Gingerol
It is converted into Cinnamic acid (2) via phenylalanine ammonia lyase (PAL). Then it is turned into p-Coumaric acid (3) with ...
Ornithine cyclodeaminase
This enzyme belongs to the family of lyases, specifically ammonia lyases, which cleave carbon-nitrogen bonds. The systematic ... and L-ornithine ammonia-lyase (cyclizing). This enzyme participates in arginine and proline biosynthesis. It employs one ... name of this enzyme class is L-ornithine ammonia-lyase (cyclizing; L-proline-forming). Other names in common use include ...
Formimidoyltetrahydrofolate cyclodeaminase
This enzyme belongs to the family of lyases, specifically ammonia lyases, which cleave carbon-nitrogen bonds. The systematic ... and 5-formimidoyltetrahydrofolate ammonia-lyase (cyclizing). This enzyme participates in folate metabolism by catabolising ... name of this enzyme class is 5-formimidoyltetrahydrofolate ammonia-lyase (cyclizing 5,10-methenyltetrahydrofolate-forming). ...
Nicholas Weise
Weise's work in general is based on industrial biotechnology including the enzyme Phenylalanine Ammonia Lyases (PALs). In 2018 ... Nigel Scrutton in which via the combination of three biocatalyst classes (phenylalanine ammonia lyase, carboxylic acid ... Amino Acids Using a Combination of Phenylalanine Ammonia Lyase and Aminomutase Biocatalysts". Adv. Synth. Catal. 359 (9): 1570 ... He then continued to read for his Doctor of Philosophy degree on Discovery and Development of Class I Lyase-like Enzymes for ...
Bacterial microcompartment
Ethanolamine is cleaved to ammonia and acetaldehyde through the action of ethanolamine-ammonia lyase, which also requires ... ethanolamine ammonia-lyase (EutBC) and the operon activator (EutR) compete for adenosyl-cobalamin in Salmonella typhimurium". ... "Crystal structure of the EutL shell protein of the ethanolamine ammonia lyase microcompartment". Proceedings of the National ... "Crystal Structures of Ethanolamine Ammonia-lyase Complexed with Coenzyme B12 Analogs and Substrates". Journal of Biological ...
5-Hydroxyferulic acid
Phenylalanine is first converted to cinnamic acid by the action of the enzyme phenylalanine ammonia-lyase (PAL). A series of ...
Strecker amino acid synthesis
Aspartic acid is produced by the addition of ammonia to fumarate using a lyase. One of the oldest methods begins with the ... Ammonia is subsequently eliminated after the protonation of the amino group, and finally the deprotonation of a hydroxyl group ... Nucleophilic substitution with ammonia then converts the alkyl bromide to the amino acid. "dl-ALANINE". Organic Syntheses. 9: 4 ... By replacing ammonia with (S)-alpha-phenylethylamine as chiral auxiliary the ultimate reaction product was chiral alanine. ...
Tsune Kosuge
This work included the earliest experimental demonstration of the important enzyme phenylalanine ammonia-lyase, which regulates ...
Cyanidin
L-phenylalanine then undergoes an elimination of the primary amine with Phenylalanine ammonia-lyase (PAL) to form cinnamate. ...
Homocysteine
Cystathionine γ-lyase then converts this double amino acid to cysteine, ammonia, and α-ketobutyrate. Bacteria and plants rely ...
Mimosine
strain TAL1145 is a C-N lyase that catabolizes L-mimosine into 3-hydroxy-4-pyridone, pyruvate, and ammonia". Amino Acids. 44 (6 ... "A Carbon-Nitrogen Lyase from Leucaena leucocephala Catalyzes the First Step of Mimosine Degradation". Plant Physiology. 164 (2 ...
Shikimic acid
The first enzyme involved is phenylalanine ammonia-lyase (PAL) that converts L-phenylalanine to trans-cinnamic acid and ammonia ...
Angelicin
The phenylalanine undergoes a non-oxidative deamination by phenylalanine ammonia-lyase (PAL) to trans-cinnamic acid. Afterwards ... Enzymes such as ammonialyases, methylases and hydroxylases then transform these amino acids to cinnamic acid derivatives which ...
Quercetin
... to 4-coumaroyl-CoA in a series of steps known as the general phenylpropanoid pathway using phenylalanine ammonia-lyase, ...
Hans Adolf Krebs
He found that citrulline acted as a catalyst in the metabolic reactions of urea from ammonia and carbon dioxide. He and ... One was malate synthase, which condenses acetate with glyoxylate to form malate, and the other was isocitrate lyase, which ...
Histidin amonijačna lijaza
... histidine ammonia-lyase". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=3034. ...
Ephedrine
A new pathway proposed suggests that phenylalanine first forms cinnamoyl-CoA via the enzymes phenylalanine ammonia-lyase and ... in that it uses anhydrous ammonia and lithium metal in the reaction. The second-most popular method uses red phosphorus and ...
Norethisterone
20-lyase, 21-hydroxylase, or 11β-hydroxylase.[51] Since it is not aromatized (and hence cannot be transformed into an ... is synthesized by partial reduction of the aromatic region of the 3-O-methyl ether of estrone with lithium in liquid ammonia, ...
Glutamine synthetase
... which reacts with ammonia, forming glutamine and inorganic phosphate. ADP and Pi do not dissociate until ammonia binds and ... EC4 Lyases (list). *EC5 Isomerases (list). *EC6 Ligases (list). *EC7 Translocases (list) ... glutamate-ammonia ligase. Active site between two monomers of glutamine synthetase from Salmonella typhimurium. Cation binding ... Ammonium, rather than ammonia, binds to GS because the binding site is polar and exposed to solvent.[7] In the second step, ...
Carbamoyl phosphate synthetase
Carboxy phosphate reacts with ammonia to give carbamic acid. In turn, carbamic acid reacts with a second ATP to give carbamoyl ... The small subunit contains the glutamine binding site and catalyses the hydrolysis of glutamine to glutamate and ammonia, which ... The carboxylphosphate then reacts with ammonia to form carbamic acid, releasing inorganic phosphate. ... or ammonia (EC 6.3.4.16) and bicarbonate.[1] This enzyme catalyzes the reaction of ATP and bicarbonate to produce carboxy ...
Taurine
The cystathionine is then converted to hypotaurine by the sequential action of three enzymes: cystathionine gamma-lyase, ... In the laboratory taurine can be produced by alkylation of ammonia with bromoethanesulfonate salts.[9] ...
Kynureninase
Histidine ammonia-lyase. *Urocanate hydratase. *Formiminotransferase cyclodeaminase. proline→. *Proline oxidase. *Pyrroline-5- ...
Histidine
The enzyme histidine ammonia-lyase converts histidine into ammonia and urocanic acid. A deficiency in this enzyme is present in ... these reactions result in the formation of glutamate and ammonia.[21] Glutamate can then be deaminated by glutamate ...
Glycine cleavage system
The ammonia generated by the glycine cleavage system, is assimilated by the Glutamine synthetase-Glutamine oxoglutarate ... HMG-CoA lyase. *HMG-CoA reductase. *Isovaleryl coenzyme A dehydrogenase. *α-Ketoisocaproate dioxygenase ... The H-protein forms a complex with the T-protein that uses tetrahydrofolate and yields ammonia and 5,10- ...
Shikimic acid
The first enzyme involved is phenylalanine ammonia-lyase (PAL) that converts L-phenylalanine to trans-cinnamic acid and ammonia ...
Tyrosine
... ammonia lyase (TAL) is an enzyme in the natural phenols biosynthesis pathway. It transforms L-tyrosine into p-coumaric ... The second utilizes enzymatic synthesis from phenolics, pyruvate, and ammonia through the use of tyrosine phenol-lyase.[29] ...
Aspartame
Phenylalanine ammonia lyase. *Stevia. References[edit]. *^ Budavari S, ed. (1989). "861. Aspartame". The Merck Index (11th ed ...
Histidinemia
The gene that encodes for Histidine ammonia-lyase (HAL) spans a roughly 25 kb and consists of 21 exons[4] located at the 12q22- ... the result of an inborn error of metabolism that may result in either an inactive or a severely reduced Histidine ammonia-lyase ...
Treoninska amonijak-lijaza
MeSH Threonine+ammonia-lyase. *v. *r. *u. Proteini: enzimi. Teme. Aktivno mesto • Alosterna regulacija • Mesto vezivanja • ...
Creatine kinase
Histidine ammonia-lyase. *Urocanate hydratase. *Formiminotransferase cyclodeaminase. proline→. *Proline oxidase. *Pyrroline-5- ...
List of enzymes
Category:EC 4.3 (carbon-nitrogen lyases)Edit. *Category:EC 4.3.1 *Phenylalanine ammonia-lyase (EC 4.3.1.24) ... Category:Lyases (EC 4) (Lyase)Edit. Category:EC 4.1 (carbon-carbon lyases)Edit. *Category:EC 4.1.1 *Ornithine decarboxylase (EC ... 4 Category:Lyases (EC 4) (Lyase) *4.1 Category:EC 4.1 (carbon-carbon lyases) ... Category:EC 4.4 (carbon-sulfur lyases)Edit. *Category:EC 4.4.1 *Cystathionine gamma-lyase ...
Capsaicin
... phenylalanine ammonia lyase (PAL), cinnamate 4-hydroxylase (C4H), caffeic acid O-methyltransferase (COMT) and their function in ... phenylalanine ammonia lyase, cinnamate 4-hydroxylase, caffeic acid O-methyltransferase. Similar studies showed high ...
Hydrogen cyanide
The most important process is the Andrussow oxidation invented by Leonid Andrussow at IG Farben in which methane and ammonia ... "Cyanogenesis in Cassava, The Role of Hydroxynitrile Lyase in Root Cyanide Production". Plant Physiology. 116 (4): 1219-1225. ... In the Shawinigan Process, hydrocarbons, e.g. propane, are reacted with ammonia. In the laboratory, small amounts of HCN are ... Andrussow, L. (1935). "The catalytic oxydation of ammonia-methane-mixtures to hydrogen cyanide". Angewandte Chemie. 48 (37): ...
Ethylenediaminetetraacetic acid
Impurities cogenerated by this route include glycine and nitrilotriacetic acid; they arise from reactions of the ammonia ... N Lyase", Applied and Environmental Microbiology, 70 (7): 3941-3947, doi:10.1128/aem.70.7.3941-3947.2004, PMC 444814, PMID ... N lyase found in Agrobacterium tumefaciens (BY6), which can be harvested on a large scale. Additionally, the reactions ...
L-DOPA
Histidine ammonia-lyase. *Urocanate hydratase. *Formiminotransferase cyclodeaminase. proline→. *Proline oxidase. *Pyrroline-5- ...
Chloroplast
... into ammonia (NH3) which supplies the plant with nitrogen to make its amino acids and nucleotides.[158] ... "Subcellular Distribution of O-Acetylserine(thiol)lyase in Cauliflower (Brassica oleracea L.) Inflorescence". Plant Physiology ...
Phenylketonuria
Biomarin is currently conducting clinical trials to investigate PEG-PAL (PEGylated recombinant phenylalanine ammonia lyase or ' ...
Nitrilase
The acylenzyme is hydrolyzed by ammonia, displacing the enzyme and forming the amide product. Most nitrilases are made up of a ... Nitrilase should not be confused with nitrile hydratase (nitrile hydro-lyase; EC 4.2.1.84) which hydrolyses nitriles to amides ... The thioimidate formed is subsequently hydrolyzed to the acylenzyme and ammonia is created as a byproduct. The acylenzyme can ... Nitrilase enzymes (nitrile aminohydrolase; EC 3.5.5.1) catalyse the hydrolysis of nitriles to carboxylic acids and ammonia, ...
L-threo-3-Methylaspartate
Methylaspartate is an unusual amino acid formed by glutamate mutase and can be metabolised by methylaspartate ammonia-lyase. It ...
Tyrosine ammonia-lyase
Tyrosine ammonia lyase (L-tyrosine ammonia-lyase, TAL or Tyrase) is an enzyme in the natural phenols biosynthesis pathway. It ... phenylalanine ammonia-lyase) EC 4.3.1.25 (phenylalanine/tyrosine ammonia-lyase) Louie GV, Bowman ME, Moffitt MC, Baiga TJ, ... "Discovery of a substrate selectivity switch in tyrosine ammonia-lyase, a member of the aromatic amino acid lyase family". ... Tyrosine+ammonia-lyase at the US National Library of Medicine Medical Subject Headings (MeSH) www.hhmi.org Biology portal v t e ...
Aspartate ammonia-lyase (IPR004708) | InterPro | EMBL-EBI
Threonine ammonia-lyase - Wikipedia
Threonine ammonia-lyase is a tetramer of identical subunits, and is arranged as a dimer of dimers. Each subunit has two domains ... Threonine ammonia-lyase is a member of the Fold Type II family, also known as the tryptophan synthase family. Though threonine ... Threonine ammonia-lyase is not found in humans. Thus, this is one example of why humans cannot synthesize all 20 proteinogenic ... In fact, threonine ammonia-lyase has been shown to exhibit some specificity towards serine and can convert serine into pyruvate ...
Histidine ammonia-lyase - Wikipedia
Histidase converts histidine into ammonia and urocanic acid. Histidine ammonia-lyase is a cytosolic enzyme catalyzing the first ... Histidine ammonia-lyase (or histidase, or histidinase) is an enzyme that in humans is encoded by the HAL gene. ... "Entrez Gene: histidine ammonia-lyase". Suchi M, Sano H, Mizuno H, Wada Y (September 1995). "Molecular cloning and structural ... Histidine+Ammonia-Lyase at the US National Library of Medicine Medical Subject Headings (MeSH) This article incorporates text ...
Ammonia-lyase | Define Ammonia-lyase at Dictionary.com
ethanolamine ammonia-lyase small subunit [Psychromonas ingrahamii 37] - Protein - NCBI
Ammonia-lyases | definition of ammonia-lyases by Medical dictionary
... ammonia-lyases explanation free. What is ammonia-lyases? Meaning of ammonia-lyases medical term. What does ammonia-lyases mean? ... Looking for online definition of ammonia-lyases in the Medical Dictionary? ... Ammonia-lyases , definition of ammonia-lyases by Medical dictionary https://medical-dictionary.thefreedictionary.com/ammonia- ... ammonia-lyases. am·mo·ni·a-ly·as·es. (ă-mōnē-ă-līās-ēz), Enzymes that remove ammonia or an amino compound nonhydrolytically ( ...
Occurrence of Phenylalanine Ammonia-Lyase (PAL) in isolated tapetum cells of Tulipa anthers | SpringerLink
Purification and Characterization of Phenylalanine Ammonia Lyase from Trichosporon cutaneum : Figure 4
A different approach to treatment of phenylketonuria: Phenylalanine degradation with recombinant phenylalanine ammonia lyase |...
A combination of oral enzyme therapy with phenylalanine ammonia lyase (PAL; EC 4.3.1.5) and controlled low protein diet might ... A different approach to treatment of phenylketonuria: Phenylalanine degradation with recombinant phenylalanine ammonia lyase. ... A different approach to treatment of phenylketonuria: Phenylalanine degradation with recombinant phenylalanine ammonia lyase ... A different approach to treatment of phenylketonuria: Phenylalanine degradation with recombinant phenylalanine ammonia lyase ...
The phenylalanine ammonia-lyase gene family in Arabidopsis thaliana
Glucosaminate ammonia-lyase | Springer for Research & Development
WikiGenes - HAL - histidine ammonia-lyase
... histidine ammonia lyase, EC 4.3.1.3) deficiency [18].. *The human gene for histidase (histidine ammonia-lyase; HAL), the enzyme ... Discovery of a substrate selectivity switch in tyrosine ammonia-lyase, a member of the aromatic amino Acid lyase family. Watts ... Tyrosine ammonia-lyase (TAL) is a recently described member of the aromatic amino acid lyase family, which also includes ... Computational analysis of the autocatalytic posttranslational cyclization observed in histidine ammonia-lyase. A comparison ...
View source for Histidine ammonia-lyase - wikidoc
Histidine ammonia-lyase homotetramer, Pseudomonas putida }} Histidine ammonia-lyase (or histidase, or ... ammonia]] and [[urocanic acid]]. == Function == Histidine ammonia-lyase is a cytosolic enzyme catalyzing the first reaction in ... Ammonia-Lyase}} {{NLM content}} {{Amino acid metabolism enzymes}} {{Carbon-nitrogen lyases}} {{Enzymes}} {{Portal bar,Molecular ... Infobox_gene}} {{enzyme , Name = histidine ammonia-lyase , EC_number = 4.3.1.3 , CAS_number = 9013-75-6 , IUBMB_EC_number = 4/3 ...
Differentiation of Japanese green tea cultivars as revealed by RFLP analysis of phenylalanine ammonia-lyase DNA | SpringerLink
Differentiation of Japanese green tea cultivars as revealed by RFLP analysis of phenylalanine ammonia-lyase DNA. * S. Matsumoto ... Differentiation of Japanese green tea cultivars as revealed by RFLP analysis of phenylalanine ammonia-lyase DNA. Theor Appl ... were analyzed to determine the process of differentiation of Japanese tea plants using phenylalanine ammonia lyase (PAL) as a ...
Expanding the substrate scope of phenylalanine ammonia-lyase from Petroselinum crispum towards styrylalanines - Organic &...
This study focuses on the expansion of the substrate scope of phenylalanine ammonia-lyase from Petroselinum crispum (PcPAL) ... Expanding the substrate scope of phenylalanine ammonia-lyase from Petroselinum crispum towards styrylalanines L. C. Bencze, A. ... Expanding the substrate scope of phenylalanine ammonia-lyase from Petroselinum crispum towards styrylalanines† ... This study focuses on the expansion of the substrate scope of phenylalanine ammonia-lyase from Petroselinum crispum (PcPAL) ...
Quantitative relationship between phenylalanine ammonia-lyase levels and phenylpropanoid accumulation in transgenic tobacco...
Phenylalanine ammonia-lyase (PAL) catalyzes the first step in phenylpropanoid synthesis. The role of PAL in pathway regulation ... Quantitative relationship between phenylalanine ammonia-lyase levels and phenylpropanoid accumulation in transgenic tobacco ... Quantitative relationship between phenylalanine ammonia-lyase levels and phenylpropanoid accumulation in transgenic tobacco ... Quantitative relationship between phenylalanine ammonia-lyase levels and phenylpropanoid accumulation in transgenic tobacco ...
Molecules | Free Full-Text | Involvement of Carbohydrate, Protein and Phenylanine Ammonia Lyase in Up-Regulation of Secondary...
... protein synthesis and L-phenylalanine ammonia lyase (PAL; EC 4.3.1.5) activity in the Malaysian medicinal herb Labisia pumila ( ... Ibrahim, M.H.; Jaafar, H.Z. Involvement of Carbohydrate, Protein and Phenylanine Ammonia Lyase in Up-Regulation of Secondary ... Involvement of Carbohydrate, Protein and Phenylanine Ammonia Lyase in Up-Regulation of Secondary Metabolites in Labisia pumila ... Involvement of Carbohydrate, Protein and Phenylanine Ammonia Lyase in Up-Regulation of Secondary Metabolites in Labisia pumila ...
Dihydroxyphenylalanine ammonia-lyase | definition of dihydroxyphenylalanine ammonia-lyase by Medical dictionary
What is dihydroxyphenylalanine ammonia-lyase? Meaning of dihydroxyphenylalanine ammonia-lyase medical term. What does ... Looking for online definition of dihydroxyphenylalanine ammonia-lyase in the Medical Dictionary? dihydroxyphenylalanine ammonia ... dihydroxyphenylalanine ammonia-lyase. dihydroxyphenylalanine ammonia-lyase. This enzyme name was retired in 2007, as ... Dihydroxyphenylalanine ammonia-lyase , definition of dihydroxyphenylalanine ammonia-lyase by Medical dictionary https://medical ...
Phenylalanine Ammonia-Lyase and Phenolic Compounds in Chamomile Tolerance to Cadmium and Copper Excess
Home » Phenylalanine Ammonia-Lyase and Phenolic Compounds in Chamomile Tolerance to Cadmium and Copper Excess ... Phenylalanine ammonia-lyase activity (PAL, EC 4.3.1.5), total phenolics, soluble proteins, malondialdehyde and metals ... Phenylalanine Ammonia-Lyase and Phenolic Compounds in Chamomile Tolerance to Cadmium and Copper Excess. ... The effects of nitrogen deficiency on selected physiological attributes, phenylalanine ammonia-lyase (PAL, EC. 4.3.1.5) ...
Biochemical studies of phenylalanine ammonia-lyase encapsulated in erythrocytes | Biochemical Society Transactions
Crystal Structures of Ethanolamine Ammonia-lyase Complexed with Coenzyme B12 Analogs and Substrates
... *. Naoki Shibata,‡§,1 Hiroko ... Ethanolamine ammonia-lyase (EAL) (EC 4.3.1.7) or ethanolamine deaminase catalyzes the AdoCbl-dependent conversion of ... N-terminal truncation of the Escherichia coli ethanolamine ammonia-lyase β-subunit does not affect the catalytic properties of ... ethanolamine ammonia-lyase. PA. 2-amino-1-propanol. SAD. single-wavelength anomalous dispersion.. ...
Highly Active and Specific Tyrosine Ammonia-Lyases from Diverse Origins Enable Enhanced Production of Aromatic Compounds in...
... tyrosine ammonia-lyases (TAL; EC 4.3.1.23), phenylalanine ammonia-lyases (PAL; EC 4.3.1.24), or phenylalanine/tyrosine ammonia- ... Maize phenylalanine ammonia-lyase has tyrosine ammonia-lyase activity. Plant Physiol 113:175-179. doi:10.1104/pp.113.1.175. ... Some enzymes had both phenylalanine ammonia-lyase activity (PAL) and tyrosine ammonia-lyase activity (TAL), while others showed ... we aimed at increasing the range of characterized phenylalanine ammonia-lyases (PAL) and tyrosine ammonia-lyases (TAL), and in ...
Novel Anther-Specific myb Genes from Tobacco as Putative Regulators of Phenylalanine Ammonia-Lyase Expression | Plant Physiology
1996) Phenlyalanine ammonia-lyase gene, structure, expression, and evolution in Nicotiana. Plant Mol Biol 30:711-722. ... Novel Anther-Specific myb Genes from Tobacco as Putative Regulators of Phenylalanine Ammonia-Lyase Expression Seungchan Yang, ... 1993) Involvement of phenylalanine ammonia-lyase in the development of pollen in broccoli (Brassica oleracea L.) Sex Plant ... Novel Anther-Specific myb Genes from Tobacco as Putative Regulators of Phenylalanine Ammonia-Lyase Expression ...
The loss of morphogenetic potential and induction of phenylalanine ammonia-lyase in suspension cultures of Phaseolus vulgaris
... of morphogenetic potential in bean suspension cultures has been investigated by measuring the amounts of phenylalanine ammonia- ... lyase activity induced in the cells when they are transferred from a medium in which they are grown and maintained to an ... The loss of morphogenetic potential and induction of phenylalanine ammonia-lyase in suspension cultures of Phaseolus vulgaris J ... Cells grown in the presence of coconut milk did not lose the ability to induce phenylalanine ammonia-lyase or xylem elements. ...
Phenylpropanoids, Phenylalanine Ammonia Lyase and Peroxidases in Elicitor-challenged Cassava (Manihot esculenta) Suspension...
Methods: Phenylalanine ammonia‐lyase (PAL), phenylpropanoids and peroxidases (POD) were investigated in elicited cassava ... Phenylpropanoids, Phenylalanine Ammonia Lyase and Peroxidases in Elicitor-challenged Cassava (Manihot esculenta) Suspension ... Phenylpropanoids, Phenylalanine Ammonia Lyase and Peroxidases in Elicitor-challenged Cassava (Manihot esculenta) Suspension ... Phenylalanine Ammonia Lyase and Peroxidases in Elicitor-challenged Cassava (Manihot esculenta) Suspension Cells and Leaves. ...
Colocalization of l-Phenylalanine Ammonia-Lyase and Cinnamate 4-Hydroxylase for Metabolic Channeling in Phenylpropanoid...
Bolwell, G.P., Sap, J., Cramer, C.L., Schuch, W., Lamb, C.J., and Dixon, R.A. (1985). L-Phenylalanine ammonia-lyase from ... Wanner, L.A., Li, G., Ware, D., Somssich, I.E., and Davis, K.R. (1995). The phenylalanine ammonia-lyase gene family in ... To determine whether isoforms of l-Phe ammonia-lyase (PAL), the first enzyme in the pathway, can associate with the next enzyme ... Allwood, E.G., Davies, D.R., Gerrish, C., Ellis, B.E., and Bolwell, G.P. (1999). Phosphorylation of phenylalanine ammonia-lyase ...
Synechocystis PCC 6803 cells heterologously expressing bacterial tyrosine ammonia lyase can use exogenous tyrosine for p...
YRC Public Data Repository - Gene Ontology - ethanolamine ammonia-lyase complex
Suppression of phenylalanine ammonia lyase expression in sugar beet by the fungal pathogen Cercospora beticola is mediated at...
"Suppression of phenylalanine ammonia lyase expression in sugar beet by the fungal pathogen Cercospora beticola is mediated at ... Suppression of phenylalanine ammonia lyase expression in sugar beet by the fungal pathogen... Schmidt, Klaus; Heberle, Brigitte ... Suppression of phenylalanine ammonia lyase expression in sugar beet by the fungal pathogen Cercospora beticola is mediated at ... Suppression of phenylalanine ammonia lyase expression in sugar beet by the fungal pathogen Cercospora beticola is mediated at ...
Low Temperature Induces the Accumulation of Phenylalanine Ammonia-Lyase and Chalcone Synthase mRNAs of Arabidopsis thaliana in...
Low Temperature Induces the Accumulation of Phenylalanine Ammonia-Lyase and Chalcone Synthase mRNAs of Arabidopsis thaliana in ... Low Temperature Induces the Accumulation of Phenylalanine Ammonia-Lyase and Chalcone Synthase mRNAs of Arabidopsis thaliana in ... Low Temperature Induces the Accumulation of Phenylalanine Ammonia-Lyase and Chalcone Synthase mRNAs of Arabidopsis thaliana in ... Low Temperature Induces the Accumulation of Phenylalanine Ammonia-Lyase and Chalcone Synthase mRNAs of Arabidopsis thaliana in ...
EnzymesEthanolamine ammonGeneAbstractCinnamic acid 4-hydroxylaseEvaluation of Phenylalanine AmmonTyrosine ammoniaForm a unique fingerprintPhenylalanine Ammonia-Lyase ExGenesVariabilisHistidine ammonia-lyasesPhenylketonuriaCharacterizationBiosynthesisAcetaldehyde and ammoniaAspartateCinnamate 4-HydroxylaseCatalysesReactionAlpha-ketobutyrate and ammoniaHistidaseActivityExpressionDeaminationTrans-cinnamic acidCatalyzesPhenylpropanoid pathwayBlood ammonia levelsAccumulationSubstratesUrocanic acidAromaticCatalyticIsoformsDefense mechanismsStabilizationThreonineAmino acid1.24AcidSynthesisDeficiencyPathwayMicrobialPlant
Enzymes19
- A number of enzymes, belonging to the lyase class, for which fumarate is a substrate have been shown [ PMID: 3282546 ] to share a short conserved sequence around a methionine which is probably involved in the catalytic activity of this type of enzymes. (ebi.ac.uk)
- The mechanism of threonine ammonia-lyase is analogous to other deaminating PLP enzymes in its use of Schiff base intermediates. (wikipedia.org)
- Any of various enzymes that remove ammonia or an amino compound by breaking the C-N bond, leaving a C=C. (dictionary.com)
- Three novel enzymes were found to have activity exclusively for phenylalanine, including an enzyme from the low-GC Gram-positive bacterium Brevibacillus laterosporus , a bacterial-type enzyme from the amoeba Dictyostelium discoideum , and a phenylalanine ammonia-lyase from the moss Physcomitrella patens (producing 230 μM cinnamic acid per unit of optical density at 600 nm [OD 600 ]) in the medium using Escherichia coli as the heterologous host). (asm.org)
- Novel tyrosine ammonia-lyases having higher reported substrate specificity than previously characterized enzymes were also identified. (asm.org)
- The enzymes were also efficient in Saccharomyces cerevisiae , where p -coumaric acid accumulation was improved 5-fold over that in strains expressing previously characterized tyrosine ammonia-lyases. (asm.org)
- Anthocyanins, which accumulate in leaves and stems in response to low temperature and changes in light intensity, are synthesized through the phenylpropanoid pathway that is controlled by key enzymes that include phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS). (plantphysiol.org)
- With the exception of cinnamate 4-hydroxylase, the enzymes which act downstream of phenylalanine ammonia lyase are encoded by small gene families in all species analyzed so far. (tuscany-diet.net)
- Crystal structure information is used to make substrate-switched amino acid ammonia lyase enzymes, including TALs, PALs and HALs. (patentsencyclopedia.com)
- Several ammonia lyases were considered as interesting enzymes to study since these enzymes are able to do a deamination on aminoacids, though different than than lysine. (chalmers.se)
- The assays provided the kinetic constants of the conversion of the natural substrate, 3‐methyl aspartate, by the mutated enzymes and their plausible activity on lysine by detecting the theoretical conversion products: 6‐aminohex‐2‐enoic acid and ammonia. (chalmers.se)
- Focusing on an enzymatic activity with demonstrated synthetic and therapeutic relevance, five new phenylalanine ammonia lyase (PAL) enzymes were discovered and characterised with respect to their potential applications. (manchester.ac.uk)
- Ammonia lyase belongs to the family of enzymes that catalyzes the deamination of amino acids. (eurekaselect.com)
- A positive correlation was found between the activity of bioagents and improvement of peroxidase and phenylalanine ammonia-lyase enzymes in onion plants to resist infection with the pathogen. (springer.com)
- 2016 ). The antioxidant enzymes peroxidase (PO), phenylalanine ammonia-lyase (PAL), and polyphenoloxidase (PPO) were mentioned as elicitors of the systemic resistance (ISR) in the plants that have a correlation with disease control (Yasmin et al. (springer.com)
- Ammonia-lyases (ALs) represent an interesting pool of targets for the ongoing research project aimed to engineer known enzymes to modify their substrate specificity and allow the biosynthesis of 6-aminohex-2-enoic acid (6-AHEA) from lysine (Lys). (snic.se)
- In the framework of a European ERA-IB project, two different types of enzymes ( ammonia lyases and aminotransferases ) have been selected as biocatalysts for the synthesis of industrially relevant amines . (bvsalud.org)
- This superfamily represents the N-terminal of the enolase and similar structural domains found in several enzymes, such as D-glucarate dehydratases, muconate-lactonizing enzymes, mandelate racemases, d-galactonate dehydratase, O-succinylbenzoate synthases and chlormuconate cycloisomerases, l-Ala-d/l-Glu epimerases, 3-methylaspartate ammonia lyase, etc [ PMID: 15581566 ]. (ebi.ac.uk)
- Thermal bifunctionality of bacterial phenylalanine aminomutase and ammonia lyase enzymes. (innspub.net)
Ethanolamine ammon3
- Ethanolamine ammonia-lyase (EAL) (EC 4.3.1.7) or ethanolamine deaminase catalyzes the AdoCbl-dependent conversion of ethanolamine (EA) to acetaldehyde and ammonia ( 3 ) ( Equation 1 ). (pubmedcentralcanada.ca)
- Ethanolamine ammonia lyase catalyzes the deamination of ethanolamine to form acetaldehyde and ammonia - a transformation that requires coenzyme 612- The function of coenzyme 612 is to abstract a hydrogen atom from C1 of the substrate, ethanolamine, to generate a free radical intermediate. (elsevier.com)
- A DNA sequence encoding the Escherichia coli (strain K12) Ethanolamine ammonia-lyase light chain, was expressed in the hosts and tags indicated. (fishersci.fi)
Gene4
- Histidine ammonia-lyase (or histidase, or histidinase) is an enzyme that in humans is encoded by the HAL gene. (wikipedia.org)
- In all plant species investigated, several copies of phenylalanine ammonia lyase gene are found, copies that probably respond differentially to internal and external stimuli. (tuscany-diet.net)
- Characterization of the phenylalanine ammonia lyase gene from the rubber tree (Hevea brasiliensis Müll. (plantprotection.pl)
- Characterization, and expression profile of a phenylalanine ammonia lyase gene from Jatropha curcas L. Molecular Biology Reports 39 (4): 3443-3452. (plantprotection.pl)
Abstract1
- Van Havere2017, author={Van Havere, Robin}, title={Engineering of 3 methylaspartate ammonia-lyases for biobased adipic acid production}, abstract={The current traditional adipic acid production is a highly polluting process. (chalmers.se)
Cinnamic acid 4-hydroxylase1
- In the early phase of infection of sugar beet (Beta vulgaris L.) leaves with the phytopathogenic fungus Cercospora beticola, a reduction in the expression of the phenylalanine ammonia lyase (BvPAL) and cinnamic acid 4-hydroxylase (BvC4H) genes was observed. (deepdyve.com)
Evaluation of Phenylalanine Ammon1
- Biochemical Evaluation of Phenylalanine Ammonia Lyase from Endemic Plant Cyathobasis fruticulosa (Bunge) Aellen. (com.hr)
Tyrosine ammonia6
- Phenylalanine and tyrosine ammonia-lyases form cinnamic acid and p -coumaric acid, which are precursors of a wide range of aromatic compounds of biotechnological interest. (asm.org)
- Lack of highly active and specific tyrosine ammonia-lyases has previously been a limitation in metabolic engineering approaches. (asm.org)
- Phenylalanine (Phe) may be converted into β-phenylalanine (β-Phe) by phenylalanine aminomutase (PAM) or deaminated to cinnamic acid (CA). Similarly, tyrosine (Tyr) may be converted to β-tyrosine by tyrosine aminomutase (TAM) or p -coumaric acid (pHCA) by tyrosine ammonia-lyase (TAL). (asm.org)
- We demonstrate that the Synechocystis PCC 6803 expressing tyrosine ammonia-lyase from Rhodobacter sphaeroides under Ptrc1O promoter produce p-coumaric acid at a rate three times higher than that under Ptrc1Ocore promoter, accounting for 18.4 +/- 1.5 mu g of p-coumaric acid per 10(8) cells (0.36 mg L-1). (diva-portal.org)
- The enzyme from monocots is also able to act as tyrosine ammonia lyase (EC 4.3.1.25), converting tyrosine to p-coumaric acid directly, (therefore without the 4-hydroxylation step), but with a lower efficiency. (tuscany-diet.net)
- 7. The recombinant amino acid ammonia lyase enzyme of claim 1, wherein the mutation is in a residue corresponding to His 89 of Rhodobacter sphaeroides Tyrosine Ammonia Lyase. (patentsencyclopedia.com)
Form a unique fingerprint1
- Fingerprint Dive into the research topics of 'Possible regulatory role of phenylalanine ammonia-lyase in the production of anthocyanins in asparagus (Asparagus officinalis L)'. Together they form a unique fingerprint. (wur.nl)
Phenylalanine Ammonia-Lyase Ex1
- Suppression of phenylalanine ammonia lyase expression in sugar beet by the fungal pathogen. (deepdyve.com)
Genes1
- The effect of thyme oil (96 μL L−1) vapours on the expression of phenylalanine ammonia-lyase (PAL) and lipoxygenase (LOX) genes, and control of anthracnose was investigated in naturally infected 'Hass' and 'Ryan' avocado fruit stored for 21 d at 7-7.5 °C, and thereafter kept for 5 d at 20 °C, simulating the supply chain conditions. (up.ac.za)
Variabilis1
- Modulating the pH Activity Profiles of Phenylalanine Ammonia Lyase from Anabaena variabilis by Modification of Center-Near Surface Residues. (semanticscholar.org)
Histidine ammonia-lyases1
- These and former results are discussed in terms of the mechanism of action of phenyalalanine and histidine ammonia lyases. (elsevier.com)
Phenylketonuria2
Characterization1
- Ranjith N. Kumavath, Ch.V. Ramana, Ch. Sasikala, Debmalya Barh, Alan Prem Kumar and Vasco Azevedo, "Isolation and Characterization of L-Tryptophan Ammonia Lyase from Rubrivivax benzoatilyticus Strain JA2", Current Protein & Peptide Science (2015) 16: 775. (eurekaselect.com)
Biosynthesis1
- One of the most promising targets is the 3-methylaspartate ammonia-lyase (MAL) that we aim to engineer to be able to perform the biosynthesis of 6-AHEA. (snic.se)
Acetaldehyde and ammonia2
- An enzyme complex that catalyzes the breakdown of ethanolamine to form acetaldehyde and ammonia. (yeastrc.org)
- Deamination of the carbinolamine affords acetaldehyde and ammonia. (elsevier.com)
Aspartate3
- Aspartate ammonia-lyase catalyses the conversion of aspartate to fumarate. (ebi.ac.uk)
- Below are the list of possible Aspartate ammonia-lyase products. (mybiosource.com)
- Also known as Aspartate ammonia-lyase (Aspartase). (mybiosource.com)
Cinnamate 4-Hydroxylase1
- To determine whether isoforms of l -Phe ammonia-lyase (PAL), the first enzyme in the pathway, can associate with the next enzyme, the endomembrane-bound cinnamate 4-hydroxylase (C4H), to facilitate channeling, we generated transgenic tobacco ( Nicotiana tabacum ) plants independently expressing epitope-tagged versions of two PAL isoforms (PAL1 and PAL2) and C4H. (plantcell.org)
Catalyses2
- Phenylalanine ammonia-lyase (PAL) catalyses the first step controlling the rate of phenylpropanoid metabolism. (escholarship.org)
- The enzyme studied in this research project is 3‐methylaspartate ammonia lyase (MAL) which catalyses the deamination reaction on 3methylaspartic acid. (chalmers.se)
Reaction10
- Histidine ammonia-lyase is a cytosolic enzyme catalyzing the first reaction in histidine catabolism, the nonoxidative deamination of L-histidine to trans-urocanic acid. (wikipedia.org)
- Furthermore, F137V-PcPAL showed superior catalytic efficiency in the ammonia elimination reaction of several racemic styrylalanine derivatives ( rac - 1a-d ) providing access to D - 1a-d by kinetic resolution, even though the D -enantiomers proved to be reversible inhibitors. (rsc.org)
- The low equilibrium constant of the ammonia addition, the poor ligand binding affinities of 2a-d , and the non-productive binding states of the unsaturated ligands 2a-d within the active sites of either wt-PcPAL or F137V-PcPAL - as indicated by molecular modeling - might be responsible for the inactivity of the PcPAL variants in the reverse reaction. (rsc.org)
- It requires no cofactors and catalyzes the reaction that links primary and secondary metabolism: the deamination of phenylalanine to trans -cinnamic acid, with the release of nitrogen as ammonia and introduction of a trans double bond between carbon atoms 7 and 8 of the side chain. (tuscany-diet.net)
- The released ammonia is probably fixed in the reaction catalyzed by glutamine synthetase. (tuscany-diet.net)
- The K m value for ammonia in the reverse reaction by using (E)-cinnamate (2) was determined to be 4.4 and 2.6M at pH 8.8 and 10, respectively. (elsevier.com)
- The isolated L-tryptophan ammonia lyase found to catalyze the reaction of L-tryptophan to produce indole 3-acrylic acid and NH 3 . (eurekaselect.com)
- 11. Steady-state rates of phenylalanine ammonia-lyase reaction in the presence of inhibitors like D-phenylalanine, cinnamic, p-coumaric, caffeic, dihydrocaffeic and phenylpyruvic acid have shown that only one molecule of each type of inhibitor binds to a molecule of the enzyme. (iisc.ac.in)
- Compound 5 is fumaric acid generated in the reaction that converts ASA to arginine (6), which is mediated by ASA lyase. (medscape.com)
- Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. (uniprot.org)
Alpha-ketobutyrate and ammonia1
- Threonine ammonia-lyase, also commonly referred to as threonine deaminase or threonine dehydratase, is an enzyme responsible for catalyzing the conversion of L-threonine into alpha-ketobutyrate and ammonia. (wikipedia.org)
Histidase2
- Histidase converts histidine into ammonia and urocanic acid. (wikipedia.org)
- Also known as Histidine ammonia-lyase (Histidase). (mybiosource.com)
Activity12
- Phenylalanine ammonia-lyase activity (PAL, EC 4.3.1.5), total phenolics, soluble proteins, malondialdehyde and metals accumulation in four-week old chamomile ( Matricaria chamomilla) plants cultivated in nutrient solution and exposed to low (3 μM) and high (60 and 120 μM) levels of cadmium (Cd) or copper (Cu) for 7 days were studied. (ebscohost.com)
- The loss of morphogenetic potential in bean suspension cultures has been investigated by measuring the amounts of phenylalanine ammonia-lyase activity induced in the cells when they are transferred from a medium in which they are grown and maintained to an induction medium. (nih.gov)
- When cells were grown in medium with only 2,4-dichlorophenoxyacetic acid for a period of 5--10 subcultures and samples were transferred to the induction medium at intervals during the subcultures, the amounts of phenylalanine ammonia-lyase activity and the number of xylem elements induced progressively declined. (nih.gov)
- In addition to the induction brought about by the presence of naphthylacetic acid and kinetin in the induction medium the cells could also be stimulated to produce phenylalanine ammonia-lyase activity by dilution at subculture. (nih.gov)
- Thus the mechanism that produced the increase in phenylalanine ammonia-lyase activity was intact but had lost its ability to respond to the hormones of the induction medium. (nih.gov)
- As a consequence, a complex pattern of phenylalanine ammonia lyase time course specific activity levels was evident. (semanticscholar.org)
- Production of l-Phenylalanine from trans-Cinnamic Acid with Rhodotorula glutinis Containing l-Phenylalanine Ammonia-Lyase Activity. (semanticscholar.org)
- The activity of phenylalanine ammonia lyase (the active portion of pegvaliase) was equal to background levels found in blank breastmilk and TRIS buffer solution. (nih.gov)
- Depending on the relative activity towards the substrates, L-tryptophan ammonia lyase converts L-tryptophan to indole 3-acrylic acid and ammonia. (eurekaselect.com)
- The main pigments found in orchid flower petals, as well as their relationships with phenylalanine ammonia-lyase (PAL) activity, were investigated. (2pressrelease.net)
- Capsaicin formation in p-fluorophenylalanine resistant and normal cell cultures of Capsicum frutescens and activity of phenylalanine ammonia lyase. (cftri.com)
- Overall, the cycle's activity is regulated by the rate of synthesis of N -acetylglutamate , the enzyme activator that initiates incorporation of ammonia into the cycle. (medscape.com)
Expression3
- Transient expression analyses of the N-terminal MYB domain demonstrated the presence of functional nuclear localization signals, and full-length NtMYBAS1 was able to activate two different phenylalanine ammonia-lyase promoters ( PALA and gPAL1 ) in tobacco leaf protoplasts. (plantphysiol.org)
- The expression of phenylalnine ammonia lyase (LJPAL1) is closely related to the content of active compounds in Lonicera japonica. (bvsalud.org)
- In this work, expression of phenylalanine ammonia-lyase (PAL) was monitored in infected leaves of two barley genotypes, Banteng (resistant) and Furat1 (susceptible) across four-time points of Pg systemic movement using qRT-PCR approach. (journal-of-agroalimentary.ro)
Deamination1
- Ammonia elimination from L -styrylalanine ( L - 1a ) catalyzed by non-mutated PcPAL (wt-PcPAL) took place with a 777-fold lower k cat / K M value than the deamination of the natural substrate, L -Phe. (rsc.org)
Trans-cinnamic acid2
- 9. The recombinant amino acid ammonia lyase enzyme of claim 1, wherein the enzyme produces trans-cinnamic acid. (patentsencyclopedia.com)
- Phenylalanine ammonia lyase (PAL) is the key enzyme that catalyzes the conversion of L-Phenylalanine to ammonia and trans- cinnamic acid. (innspub.net)
Catalyzes1
- Phenylalanine ammonia-lyase (PAL) catalyzes the first step in phenylpropanoid synthesis. (pnas.org)
Phenylpropanoid pathway1
- Phenylalanine ammonia-lyase [PAL] is the first entry enzyme of the phenylpropanoid pathway that converts phenylalanine to cinnamic acid which is the precursor of various secondary metabolites. (who.int)
Blood ammonia levels2
- She, who was diagnosed as ASL deficiency after the laboratory examinations, had normal blood ammonia levels. (bezmialem.edu.tr)
- Failure to suspect hyperammonemia and to obtain blood ammonia levels results in certain morbidity and, likely, death because routine laboratory test findings are unrevealing. (medscape.com)
Accumulation1
- The regulatory role of phenylalanine ammonia-lyase (PAL) in the light-induced accumulation of anthocyanins in the epidermis of asparagus spears has been analysed. (wur.nl)
Substrates2
- N-Methyl-L-phenylalanine (5), N-methyl-4-nitro-L-phenylalanine (6), and N,N-dimethyl-4-nitro-L-phenyl-alanine (7·H + ) were investigated as substrates of inhibitors of phenylalanine ammonia lyase from Petroselinum crispum. (elsevier.com)
- The present study was conducted with the main objective to optimize the phenylalanine ammonia lyase (PAL) production process by Bacillus subtilis GCB-31 on agro-industrial wastes as carrier substrates in solid state fermentation. (innspub.net)
Urocanic acid1
- The pharmacokinetics of L-histidine in humans has been investigated to evaluate the in vivo histidine ammonia lyase system for the conversion of L-histidine to urocanic acid. (aspetjournals.org)
Aromatic4
- This study focuses on the expansion of the substrate scope of phenylalanine ammonia-lyase from Petroselinum crispum (PcPAL) towards the L -enantiomers of racemic styrylalanines rac - 1a-d - which are less studied and synthetically challenging unnatural amino acids - by reshaping the aromatic binding pocket of the active site of PcPAL by point mutations. (rsc.org)
- Aromatic amino acid ammonia-lyases and aminomutases. (asm.org)
- 3. The recombinant amino acid ammonia lyase enzyme of claim 2, wherein the first and second amino acids are aromatic amino acids. (patentsencyclopedia.com)
- We also show that the identity of the amino acid at the switch position can serve as a guide to predict substrate specificities of annotated aromatic amino acid lyases in genome sequences. (umn.edu)
Catalytic1
- Structural and catalytic properties of the four phenylalanine ammonia-lyase isoenzymes from parsley (Petroselinum crispum Nym. (plantprotection.pl)
Isoforms1
- Two phenylalanine ammonia lyase isoforms are involved in the elicitor-induced response of rice to the fungal pathogen Magnaporthe oryzae. (semanticscholar.org)
Defense mechanisms1
- In plants, threonine ammonia-lyase is important in defense mechanisms against herbivores and is upregulated in response to abiotic stress. (wikipedia.org)
Stabilization1
- Stabilization of phenylalanine ammonia lyase containing Rhodotorulaglutinis cells for the continuous synthesis of L-phenylalanine methyl ester/96. (innspub.net)
Threonine4
- Alpha-ketobutyrate can be converted into L-isoleucine, so threonine ammonia-lyase functions as a key enzyme in BCAA synthesis. (wikipedia.org)
- Threonine ammonia-lyase is a tetramer of identical subunits, and is arranged as a dimer of dimers. (wikipedia.org)
- Threonine ammonia-lyase has been shown to not follow Michaelis-Menten kinetics, rather, it is subject to complex allosteric control. (wikipedia.org)
- Multiple forms of threonine ammonia-lyase have been observed in a variety of species of organism. (wikipedia.org)
Amino acid7
- 1. A recombinant amino acid ammonia lyase enzyme, comprising at least one mutation in an active site of the enzyme, wherein the mutation switches substrate preference of the lyase enzyme from a first substrate to a second substrate. (patentsencyclopedia.com)
- 2. The recombinant amino acid ammonia lyase enzyme of claim 1, wherein the first substrate is an amino acid, and the second substrate is an amino acid. (patentsencyclopedia.com)
- 5. The recombinant amino acid ammonia lyase enzyme of claim 3, wherein the first amino acid is tyrosine or histidine and the second amino acid is phenylalanine. (patentsencyclopedia.com)
- 6. The recombinant amino acid ammonia lyase enzyme of claim 1, wherein the recombinant enzyme is derived from a tyrosine or histidine ammonia lyase, and wherein the recombinant enzyme preferentially deaminates L-Phe. (patentsencyclopedia.com)
- 8. The recombinant amino acid ammonia lyase enzyme of claim 1, wherein the enzyme comprises a 4-methylidene-imidazole-5-one (MOI) cofactor prosthetic group. (patentsencyclopedia.com)
- 12. The recombinant cell of claim 11, wherein the cell encodes a recombinant tyrosine amino acid-type ammonia lyase enzyme that comprises a mutation converting a kinetic preference of the enzyme for tyrosine into a preference for phenylalanine. (patentsencyclopedia.com)
- 17. A library of amino acid ammonia lyase polypeptides, the library comprising:a plurality of polypeptides comprising or derived from amino acid ammonia lyase enzyme polypeptides, wherein the plurality of polypeptides collectively comprise a plurality of mutations of at least one amino acid in at least one region of the polypeptides, the region corresponding to an active site of an amino acid ammonia lyase enzyme. (patentsencyclopedia.com)
1.241
- EC: 4.3.1.24) of the thermophilic and radiotolerant bacterium Rubrobacter xylanophilus (RxPAL) was identified by screening the genomes of bacteria for members of the phenylalanine ammonia-lyase family. (elsevier.com)
Acid3
- Phenylalanine ammonia lyases (PALs) catalyse the regio- and stereoselective hydroamination of cinnamic acid analogues to yield optically enriched α-amino acids. (manchester.ac.uk)
- They learned that the critical element in this synthesis was an intermediary enzyme that deaminates phenylalanine to cinnamic acid and ammonia (later called phenylalanine ammonia lyase or PAL). (cdc.gov)
- The relationship between the formation of cell wall-bound ferulic acid (FA) and diferulic acid (DFA) and the change in activities of phenylalanine ammonia-lyase (PAL) and cell wall-bound peroxidase (CW-PRX) was studied in rice shoots. (spacestationresearch.com)
Synthesis1
- Synthesis and degradation of phenylalanine ammonia-lyase of Rhodosporidium toruloides. (semanticscholar.org)
Deficiency8
- The effects of nitrogen deficiency on selected physiological attributes, phenylalanine ammonia-lyase (PAL, EC. (ebscohost.com)
- Arginine succinate lyase (ASL) deficiency is one of the most common cause of urea cycle defect and shows all characteristics of this disorders. (bezmialem.edu.tr)
- ASL deficiency must be thought when a child has mental retardation and cerebellar ataxia, even if normal ammonia levels. (bezmialem.edu.tr)
- Argininosuccinate (ASA) lyase deficiency results in defective cleavage of ASA. (medscape.com)
- [ 1 ] The most important characteristic of ASA lyase deficiency is its propensity to cause hyperammonemia in affected individuals. (medscape.com)
- [ 7 ] According a study of urea cycle diseases in Finland, 20 cases of ASA lyase deficiency had been reported by 2007. (medscape.com)
- ASA lyase deficiency is associated with high mortality and morbidity rates. (medscape.com)
- Although intellectual impairment is the rule, even among patients who receive excellent and timely treatment, some patients with ASA lyase deficiency reportedly develop normally. (medscape.com)
Pathway1
- The profile of phenylalanine ammonia lyase (PAL), the key enzyme in pheny1propanoid pathway in resistant cell cultures was studied and compared with normal cell cultures to understand its role in capsaicin formation. (cftri.com)
Microbial2
- Microbial L-phenylalanine ammonia-lyase. (semanticscholar.org)
- 2014. Biotechnological production and applications of microbial phenylalanine ammonia lyase: a recent review. (innspub.net)
Plant2
- The journey of phenylalanine ammonia lyase (PAL) from plant enzyme to enzyme substitution therapy for PKU is a fascinating story that illustrates the importance of collaboration between basic scientists and industry in the drug development process. (cdc.gov)
- Phenylalanine ammonia-lyase (EC 4.3.1.5) was purified to homogeneity from the acetone-dried powders of the mycelial felts of the plant pathogenic fungus Rhizoctonia solani. (iisc.ac.in)