A pyrazolone with analgesic, anti-inflammatory, and antipyretic properties but has risk of AGRANULOCYTOSIS. A breath test with 13C-labeled aminopyrine has been used as a non-invasive measure of CYTOCHROME P-450 metabolic activity in LIVER FUNCTION TESTS.
An NADPH-dependent P450 enzyme that plays an essential role in the sterol biosynthetic pathway by catalyzing the demethylation of 14-methyl sterols such as lanosterol. The enzyme acts via the repeated hydroxylation of the 14-methyl group, resulting in its stepwise conversion into an alcohol, an aldehyde and then a carboxylate, which is removed as formic acid. Sterol 14-demethylase is an unusual cytochrome P450 enzyme in that it is found in a broad variety of organisms including ANIMALS; PLANTS; FUNGI; and protozoa.
Enzymes that catalyse the removal of methyl groups from LYSINE or ARGININE residues found on HISTONES. Many histone demethylases generally function through an oxidoreductive mechanism.
A family of histone demethylases that share a conserved Jumonji C domain. The enzymes function via an iron-dependent dioxygenase mechanism that couples the conversion of 2-oxoglutarate to succinate to the hydroxylation of N-methyl groups.
A drug-metabolizing, cytochrome P-450 enzyme which catalyzes the hydroxylation of aniline to hydroxyaniline in the presence of reduced flavoprotein and molecular oxygen. EC 1.14.14.-.
Rounded or pyramidal cells of the GASTRIC GLANDS. They secrete HYDROCHLORIC ACID and produce gastric intrinsic factor, a glycoprotein that binds VITAMIN B12.
Closed vesicles of fragmented endoplasmic reticulum created when liver cells or tissue are disrupted by homogenization. They may be smooth or rough.
A retinoblastoma binding protein that is also a member of the Jumonji-domain histone demethylases. It has demethylation activity towards specific LYSINE residues found on HISTONE H3.
A superfamily of hundreds of closely related HEMEPROTEINS found throughout the phylogenetic spectrum, from animals, plants, fungi, to bacteria. They include numerous complex monooxygenases (MIXED FUNCTION OXYGENASES). In animals, these P-450 enzymes serve two major functions: (1) biosynthesis of steroids, fatty acids, and bile acids; (2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs (BIOTRANSFORMATION). They are classified, according to their sequence similarities rather than functions, into CYP gene families (>40% homology) and subfamilies (>59% homology). For example, enzymes from the CYP1, CYP2, and CYP3 gene families are responsible for most drug metabolism.
Addition of methyl groups. In histo-chemistry methylation is used to esterify carboxyl groups and remove sulfate groups by treating tissue sections with hot methanol in the presence of hydrochloric acid. (From Stedman, 25th ed)
Small chromosomal proteins (approx 12-20 kD) possessing an open, unfolded structure and attached to the DNA in cell nuclei by ionic linkages. Classification into the various types (designated histone I, histone II, etc.) is based on the relative amounts of arginine and lysine in each.
Hydrochloric acid present in GASTRIC JUICE.
Oxidative enzyme which transforms p-nitroanisole into p-nitrophenol.
Drug metabolizing enzymes which oxidize methyl ethers. Usually found in liver microsomes.
An analgesic and antipyretic that has been given by mouth and as ear drops. Antipyrine is often used in testing the effects of other drugs or diseases on drug-metabolizing enzymes in the liver. (From Martindale, The Extra Pharmacopoeia, 30th ed, p29)
A drug that has analgesic, anti-inflammatory, and antipyretic properties. It is the sodium sulfonate of AMINOPYRINE.
An inhibitor of drug metabolism and CYTOCHROME P-450 ENZYME SYSTEM activity.
The removing of alkyl groups from a compound. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
A barbituric acid derivative that acts as a nonselective central nervous system depressant. It potentiates GAMMA-AMINOBUTYRIC ACID action on GABA-A RECEPTORS, and modulates chloride currents through receptor channels. It also inhibits glutamate induced depolarizations.
A barbiturate that is effective as a hypnotic and sedative.
A group of compounds that contain a bivalent O-O group, i.e., the oxygen atoms are univalent. They can either be inorganic or organic in nature. Such compounds release atomic (nascent) oxygen readily. Thus they are strong oxidizing agents and fire hazards when in contact with combustible materials, especially under high-temperature conditions. The chief industrial uses of peroxides are as oxidizing agents, bleaching agents, and initiators of polymerization. (From Hawley's Condensed Chemical Dictionary, 11th ed)
Compounds with a five-membered heterocyclic ring with two nitrogens and a keto OXYGEN. Some are inhibitors of TNF-ALPHA production.
A triterpene that derives from the chair-boat-chair-boat folding of 2,3-oxidosqualene. It is metabolized to CHOLESTEROL and CUCURBITACINS.
A barbiturate that is used as a sedative. Secobarbital is reported to have no anti-anxiety activity.
A flavoprotein that catalyzes the reduction of heme-thiolate-dependent monooxygenases and is part of the microsomal hydroxylating system. EC 1.6.2.4.
A hypnotic and sedative. Its use has been largely superseded by other drugs.
A saclike, glandular diverticulum on each ductus deferens in male vertebrates. It is united with the excretory duct and serves for temporary storage of semen. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Artifactual vesicles formed from the endoplasmic reticulum when cells are disrupted. They are isolated by differential centrifugation and are composed of three structural features: rough vesicles, smooth vesicles, and ribosomes. Numerous enzyme activities are associated with the microsomal fraction. (Glick, Glossary of Biochemistry and Molecular Biology, 1990; from Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
An enzyme that catalyzes the methylation of the epsilon-amino group of lysine residues in proteins to yield epsilon mono-, di-, and trimethyllysine. EC 2.1.1.43.
Widely distributed enzymes that carry out oxidation-reduction reactions in which one atom of the oxygen molecule is incorporated into the organic substrate; the other oxygen atom is reduced and combined with hydrogen ions to form water. They are also known as monooxygenases or hydroxylases. These reactions require two substrates as reductants for each of the two oxygen atoms. There are different classes of monooxygenases depending on the type of hydrogen-providing cosubstrate (COENZYMES) required in the mixed-function oxidation.
An essential amino acid. It is often added to animal feed.
Lining of the STOMACH, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. The surface cells produce MUCUS that protects the stomach from attack by digestive acid and enzymes. When the epithelium invaginates into the LAMINA PROPRIA at various region of the stomach (CARDIA; GASTRIC FUNDUS; and PYLORUS), different tubular gastric glands are formed. These glands consist of cells that secrete mucus, enzymes, HYDROCHLORIC ACID, or hormones.
Any tests done on exhaled air.
A genetic process by which the adult organism is realized via mechanisms that lead to the restriction in the possible fates of cells, eventually leading to their differentiated state. Mechanisms involved cause heritable changes to cells without changes to DNA sequence such as DNA METHYLATION; HISTONE modification; DNA REPLICATION TIMING; NUCLEOSOME positioning; and heterochromatization which result in selective gene expression or repression.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
An amine derived by enzymatic decarboxylation of HISTIDINE. It is a powerful stimulant of gastric secretion, a constrictor of bronchial smooth muscle, a vasodilator, and also a centrally acting neurotransmitter.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.
A flavoring agent. It is the intermediate product in the two-step bioconversion of ferulic acid to vanillin. (J Biotechnol 1996;50(2-3):107-13).
A family of proteins that share the F-BOX MOTIF and are involved in protein-protein interactions. They play an important role in process of protein ubiquition by associating with a variety of substrates and then associating into SCF UBIQUITIN LIGASE complexes. They are held in the ubiquitin-ligase complex via binding to SKP DOMAIN PROTEINS.
A sympathomimetic agent with properties similar to DEXTROAMPHETAMINE. It is used in the treatment of obesity. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1222)
A carcinogen that is often used in experimental cancer studies.
The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)
An increase in the rate of synthesis of an enzyme due to the presence of an inducer which acts to derepress the gene responsible for enzyme synthesis.
Five membered rings containing a NITROGEN atom.
A propylamine formed from the cyclization of the side chain of amphetamine. This monoamine oxidase inhibitor is effective in the treatment of major depression, dysthymic disorder, and atypical depression. It also is useful in panic and phobic disorders. (From AMA Drug Evaluations Annual, 1994, p311)
Sulfur compounds in which the sulfur atom is attached to three organic radicals and an electronegative element or radical.
A megaloblastic anemia occurring in children but more commonly in later life, characterized by histamine-fast achlorhydria, in which the laboratory and clinical manifestations are based on malabsorption of vitamin B 12 due to a failure of the gastric mucosa to secrete adequate and potent intrinsic factor. (Dorland, 27th ed)
Peroxides produced in the presence of a free radical by the oxidation of unsaturated fatty acids in the cell in the presence of molecular oxygen. The formation of lipid peroxides results in the destruction of the original lipid leading to the loss of integrity of the membranes. They therefore cause a variety of toxic effects in vivo and their formation is considered a pathological process in biological systems. Their formation can be inhibited by antioxidants, such as vitamin E, structural separation or low oxygen tension.

Role of C-5 chiral center in R-(+)-pulegone-mediated hepatotoxicity: metabolic disposition and toxicity of 5, 5-dimethyl-2-(1-Methylethylidene)-cyclohexanone in rats. (1/55)

Metabolic disposition of 5, 5-dimethyl-2-(1-methylethylidene)-cyclohexanone (I) was examined in rats. Compound (I) was administered orally (250 mg/kg of body weight/day) to rats for 5 days. The following urinary metabolites were isolated and identified: 4,5,6,7-tetrahydro-3,6, 6-trimethylbenzofuran (III), 3,3-dimethylcyclohexanone (VI), 5, 5-dimethyl-3-hydroxy-2-(1-methylethylidene)-cyclohexanone (X), 5, 5-dimethyl-2-(1-hydroxymethylethyl)-cyclohexanone (IX), 3-hydroxy-5-hydroxymethyl-5-methyl-2-(1-methylethylidene)-cyclo hexano ne (XI), 5,6-dihydro-3,6,6-trimethyl-2(4H)-benzofuranone (VIII), and 5,5-dimethyl-3-hydroxy-2-(1-carboxy ethylidene)-cyclohexanone (XIII). Incubation of compound (I) with phenobarbital (PB)-induced rat liver microsomes in the presence of NADPH resulted in the formation of a metabolite, tentatively identified as a furanoterpene (III) based on proton magnetic resonance, gas chromatography, and gas chromatography-mass spectroscopy analyses. The formation of III was inhibited to a significant extent by carbon monoxide, metyrapone, SKF 525-A, and cytochrome c, suggesting the participation of PB-induced microsomal cytochrome P-450 system in the conversion of I to III. Compound I gave type I spectral change in the PB-induced liver microsomes and the dissociation constant (Ks) for I was 38.5 microM. Intraperitoneal administration of a single dose (250 mg/kg) of I to rats resulted in 26, 23, and 41% decreases in the levels of cytochrome P-450, glucose-6-phosphatase, and aminopyrine N-demethylase, respectively, at the end of 24 h. During this period, a 11-fold increase in serum glutamate pyruvate transaminase level was also observed. However, a decrease in the level of cytochrome P-450 and glucose-6-phosphatase, and an increase in serum glutamate pyruvate transaminase values were comparatively more pronounced when R-(+)-pulegone (250 mg/kg) or CCl(4) (0.6 ml/kg) was administered to rats. Pretreatment of rats with PB potentiated the hepatotoxicity caused by I, whereas pretreatment with 3-methylcholanthrene protected from it. This suggests that PB-induced cytochrome P-450-catalyzed reactive metabolites may be responsible for the toxic effects caused by I.  (+info)

Studies on the formation of lipid peroxides and on some enzymic activities in the liver of vitamin E-deficient rats. (2/55)

Rats were fed a 5 or 20% casein diet that causes liver necrosis unless supplemented with vitamin E or selenite. The following activities were studied in liver subcellar fractions: enzymic formation of lipid peroxides, NADPH-cytochrome c reductase, oxidative demethylation of aminopyrine, and incorporation of [14C]leucine into protein (with microsomes); xanthine oxidase (with soluble supernatant); and RNA polymerases I and II (with nuclei). Formation of lipid peroxides was higher in rats fed diets without vitamin E and was not reduced significantly by dietary selenite. The activity of xanthine oxidase was higher in animals fed the 20% casein than in those fed the 5% casein diet; however, a higher activity was observed in the rats fed the latter diet without vitamin E or selenite than in those receiving these supplements. The activity of RNA polymerase I was higher in rats fed the low casein diet. Other activities examined were not affected significantly by the level of dietary casein or by vitamin E or selenits.  (+info)

Effect of repeated exposure to aniline, nitrobenzene, and benzene on liver microsomal metabolism in the rat. (3/55)

Exposure of rats to aniline at daily doses of 50 mg/kg of body weight over a month stimulated the microsomal metabolism as manifested by (1) acceleration of p-hydroxylation of anilin and N-demethylation of aminopyrine in 9-000 times g postmitochondrial supernatant of the liver, (2) shortening the sleeping time after hexobarbital, and (3) reduction of the antipyretic effect of phenacetin. In the rats exposed to nitrobenzene in a similar manner to aniline, nitroreduction of nitrobenzene and p-hydroxylation of aniline remained unaffected; the antipyretic effect of phenacetin was decreased, whereas hexobarbital sleeping time remained unchanged. Exposure of rats to benzene (50 mg/kg of body weight daily for a month) had no effect on the rate of hydroxylation of benzene and N-demethylation of aminopyrine. In benzene-exposed rats hexobarbital sleeping time was prolonged whereas the antipyretic effect of phenacetin was unaffected. Microsomal metabolism of aniline, nitrobenzene, and benzene was stimulated and inhibited when the rats were pretreated with phenobarbital and SKF 525-A, respectively.  (+info)

Induction of drug metabolism-related enzymes by methylcholanthrene and phenobarbital in transgenic mice carrying human prototype c-Ha-ras gene and their wild type littermates. (4/55)

Transgenic mice hemizygously carrying human c-Ha-ras proto-oncogene, Tg-rasH2 show very sensitive and facilitated carcinogenicity to various carcinogens. In this study, activities of certain enzymes related to drug metabolism and energy metabolism were measured in microsome and cytosol fractions of livers of Tg-rasH2 mice and their wild type littermates with both sexes treated with 3-methylcholanthrene (MC) and phenobarbital (PB). Aminopyrine N-demethylase activities increased significantly in livers of all mice treated with PB. MC and PB treatments induced significant increases in activities of UDP-glucuronosyltransferase and S-adenosyl homocysteinase compared to those in the non-treated groups in microsome fractions from all mice. In cytosol fractions of livers of all mice, glutathione S-transferase activity was significantly induced in the PB treated groups. There were no significant differences in activities of lactate dehydrogenase, glucose 6-phosphate dehydrogenase, pyruvate kinase and glucose 6-phosphatase related to energy metabolism in livers and kidneys among all mice. Tg-rasH2 mice showed stable activities of enzymes related to drug detoxication and energy metabolism similar to those of non-transgenic mice. These results suggest that the human c-Ha-ras transgene may not affect drug metabolism-related enzymes, and the facilitated carcinogenic response in the Tg-rasH2 mouse is not due to these enzymatic disorders.  (+info)

Further experiments on lipid peroxidation in transplanted and experimental hepatomas. (5/55)

The results of experiments on the subject of lipid peroxidation in hepatomas are described. It is now clear that lipid peroxidation is strongly decreased in most highly dedifferentiated hepatomas. It seems evident that the extent of the decline is strictly related to the degree of dedifferentiation. The model of diethylnitrosamine carcinogenesis, according to the method by Solt, Medline and Farber, has been now adopted to study the stages of carcinogenesis. It was shown that a net decline in lipid peroxidation occurs as early as at the stage of reversible nodules and progresses until the development of clear hepatomas. This change is practically simultaneous with a decline in the efficiency of the enzymes of the drug metabolizing system and in the content of cytochrome P450-Glutathione content and metabolism show also important changes. In fact, a dramatic increase in gamma-glutamyl-transpeptidase takes place very early during carcinogenesis, and is responsible for large decline in total glutathione during incubation of the homogenates. Glutathione peroxidase activity, on the contrary, is decreased, whereas glutathione reductase does not show significant changes. The supernatant of highly anaplastic tumors inhibits lipid peroxidation in normal liver homogenates, suggesting the presence of substances provided with antioxidant properties. These cannot be, however, related to a higher glutathione content. Supernatants from early nodules seem to be unable to block lipid peroxidation in normal liver homogenates. Preliminary experiments done to study the aldehyde pattern produced during lipid peroxidation, both in hepatomas and in nodules, confirm the presence of very poor lipid peroxidation and possibly of different peroxidation kinetics.  (+info)

Studies on the evaluation of the toxicity of various salts of lead, manganese, platinum, and palladium. (6/55)

Preliminary studies have been conducted on various parameters in order to assess the possible and relative toxicities of a number of metallic salts. Upon oral administration in lethal-dose experiments, two soluble Pt4+ salts were more toxic than the other salts tested. Following intraperiotneal injection in lethal-dose experiments, PbCl2 was less toxic than several of the soluble or partially soluble salts of Pt4+, Pd2+, and Mn2+. An intake of a total of approximately 250 mg of Pt4+ per rat in the drinking fluid over a 30-day interval did not affect the activities of aniline hydroxylase and aminopyrine demethylase in rat liver microsomes. In rats receiving soluble Pt4+ salts in the drinking fluid, the highest concentration of Pt was found in the kidney and an appreciiable concentration was found in the liver.  (+info)

Inhibition of human hepatic cytochrome P450s and steroidogenic CYP17 by nonylphenol. (7/55)

Effect of nonylphenol on aminopyrine N-demethylase activity, a typical drug-metabolizing enzyme activity, by ten kinds of human hepatic cytochrome P450s (CYP) and on progesterone 17alpha-hydroxylase activity by steroidogenic CYP17 was investigated. When determined at 2 mM substrate concentration, nonylphenol (1 mM) most efficiently inhibited aminopyrine N-demethylation by CYP2C9 and CYP2C19, by 61% and 59%, respectively, followed by CYP2D6, CYP1A2, CYP2C18 and CYP2C8 (46-51%), whereas inhibition of the activities by other CYPs was less than 27%. Additionally, nonylphenol competitively inhibited diclofenac 4'-hydroxylation by CYP2C9 and S-mephenytoin 4'-hydroxylation by CYP2C19 with Ki values of 5.3 and 37 microM, respectively. Furthermore, nonylphenol exhibited a competitive inhibition of progesterone 17alpha-hydroxylase activity by CYP17 with Ki value of 62 microM. These results suggest that nonylphenol inhibits human hepatic CYPs, especially CYP2C9 and CYP2C19, and steroidogenic CYP17 activities.  (+info)

Hepatic microsomal enzyme induction in rats fed varietal cauliflower leaves. (8/55)

Leaves from a standard, insect-susceptible cauliflower variety and an insect-resistant strain were formulated at either 10 or 25% into semipurified diets for male and female weanling rats. After 3 weeks, relative liver weights, microsomal protein, cytochrome P-450, and activities of hepatic microsomal aminopyrine N-demethylase, aniline hydroxylase, p-nitroanisole O-demethylase, and N-methylaniline N-demethylase were determined. Growth, feed intake, and feed efficiency of male rats were not affected by the inclusion of the dried cauliflower leaf in the diet. However, female rats exhibited a depressed feed intake and increased feed efficiency with cauliflower leaf supplemental diets. Relative liver weights increased with increasing percentage of cauliflower leaves in the diet. Hepatic microsomal enzyme response to cauliflower leaf supplementation of the diet was greater in males than in females. Only aniline hydroxylase activity remained unchanged by the test diets. Male rats showed significant increases in N- and O-demethylation with both the 10 and 25% cauliflower diets, and increased values for microsomal protein and cytochrome P-450 at the 25% supplemental level. Female rats did not show significant hepatic microsomal induction from cauliflower leaf consumption at the 10% level. However, cytochrome P-450 and the metabolism of aminopyrine and p-nitroanisole were enhanced by consumption of cauliflower leaves at 25% of their diet. None of the parameters tested in this study evidenced a difference between the two cauliflower cultivars fed to either sex.  (+info)

The development of several hepatic microsomal drug-metabolizing enzyme activities in rats was studied in relation to its androgen dependence during the neonatal period. Rats with their androgen deprived during the neonatal period (female rats and male rats castrated at birth) respond less to androgen treatment at adulthood for the metabolism of aminopyrine, ethylmorphine and hexobarbital as compared to rats exposed to neonatal androgen (male rats, male rats castrated at birth but neonatally treated with androgen and male rats castrated at the age of 20 days). This difference in the degree of hepatic responsiveness, however, varied with the substrates: ethylmorphine N-demethylase activity was affected the most whereas aminopyrine N-demethylase and hexobarbital oxidase activities were only marginally affected. Furthermore, these differences in responsiveness seem to be a delayed event, since hepatic aminopyrine N-demethylase activity in rats castrated at birth did respond to androgen stimulation ...
The aim of this study was to investigate the possible effects of sulphite oxidase (SOX, E.C. 1.8.3.1) deficiency on xenobiotic metabolism. For this purpose, SOX deficiency was produced in rats by the administration of a low molybdenum diet with concurrent addition of 200 ppm tungsten to their drinking water. First, hepatic SOX activity in deficient groups was measured to confirm SOX deficiency. Then, aminopyrine N-demethylase, aniline 4-hydroxylase, aromatase, caffeine N-demethylase, cytochrome b5 reductase, erythromycin N-demethylase, ethoxyresorufin O-deethylase, glutathione S-transferase, N-nitrosodimethylamine N-demethylase and penthoxyresorufin O-deethylase activities were determined to follow changes in the activity of drug metabolizing enzymes in SOX-deficient rats. Our results clearly demonstrated that SOX deficiency significantly elevated A4H, caffeine N-demethylase, erythromycin N-demethylase and N-nitrosodimethylamine N-demethylase activities while decreasing ethoxyresorufin ...
We have previously described the development of genetic models to study the in vivo functions of the hepatic cytochrome P450 system, through the hepatic deletion of either cytochrome P450 oxidoreductase (POR; HRN line) or cytochrome b5 (Cyb5; HBN line). However, HRN mice still exhibit low levels of mono-oxygenase activity, in spite of the absence of detectable reductase protein. To investigate whether this is because cytochrome b5 and cytochrome b5 reductase can act as sole electron donors to the P450 system, we have crossed HRN with HBN mice to generate a line lacking hepatic expression of both electron donors (HBRN). HBRN mice exhibited exacerbation of the phenotypic characteristics of the HRN line - liver enlargement, hepatosteatosis and increased expression of certain cytochrome P450s. Also, drug metabolising activities in vitro were further reduced relative to the HRN model, in some cases to undetectable levels. Pharmacokinetic studies in vivo demonstrated that midazolam half-life, Cmax and ...
Biochemical, histopathological and ultrastructural changes occurring at different time points after intraperitoneal administration of a single dose of pulegone (300 mg/kg) were studied. Significant decreases in the level of liver microsomal cytochrome P-450 (67%), heme (37%), aminopyrine N-demethylase (60%) and glucose-6-phosphatase (58%), were noticed 24 hr after pulegone treatment. Alanine amino transferase (ALT) levels increased in a time dependent manner, following exposure of rats to pulegone. Light microscopic studies of liver tissues showed dilation of central veins and distention of sinusoidal spaces 6 hr after pulegone treatment. Initial centrilobular necrosis was noticed at 12 hr. Centrilobular necrosis became severe at 18 hr and nuclear changes included karyorrhexis and karyolysis. Midzonal and periportal degenerative changes in addition to centrilobular necrosis was observed 24 hr after pulegone administration. Electron microscopic changes showed severe degeneration of endoplasmic ...
2 major biochemical reactions that occur in the peroxisome is the mixed function oxidase and the other is a catalase but what 2 enzymes are involved in these reactions?...beta oxidation one of them ...
The effect of a 20-day administration of aminopyrine (600 mg kg-1) as well as two metabolites of aminopyrine, 4-aminoantipyrine (525 mg kg-1) and 4-acetamidoantipyrine (635 mg kg-1), on several hepatic, kidney and serum enzyme activities were investigated. In the aminopyrine-treated group, a pronounced induction of gamma-glutamyl transpeptidase was shown in the whole homogenate as compared to that in the hepatic microsomes. Serum gamma-glutamyl transpeptidase activity was also increased by administration of aminopyrine or 4-aminoantipyrine. No change in gamma-glutamyl transpeptidase activity was observed in kidney and hepatic cytosolic fractions. In all cases, 4-acetamidoantipyrine treatment showed no significant change in the enzyme activities tested. Under the same experimental condition, the amounts of cytochrome P-450 and b5, the activities of aminopyrine N-demethylase, aniline hydroxylase and carboxylesterase of liver microsomes were all induced in the aminopyrine- and ...
Clotrimazole, an N-substituted imidazole widely used as an antifungal agent, has been shown to both inhibit and induce hepatic cytochrome P-450 and related monooxygenase activities. In this study the profile of hepatic cytochrome P-450 isozyme(s) induced by clotrimazole treatment of male Sprague-Dawley rats was investigated. Clotrimazole administration (100 mg/kg, daily for 4 days, ig) resulted in 86% induction of spectrally detectable cytochrome P-450 in hepatic microsomes. In these microsomes 7-ethoxycoumarin O-deethylase (126%), aminopyrine N-demethylase (176%), benzphetamine N-demethylase (117%), p-nitrophenol hydroxylase (89%), and 7-ethoxyresorufin O-deethylase (62%) activities were significantly induced, whereas aryl hydrocarbon hydroxylase activity remained unchanged. Characterization of cytochrome P-450 isozyme(s) in hepatic microsomes prepared from clotrimazole-treated animals was based on the immunoreactivity of these microsomes with highly specific monoclonal antibodies (MAbs) raised ...
MARUMO, Sativa (1997) Regio- and stereoselective propranolol metabolism by 3 forms of purified cytochrome P450 from rat liver and the effect of cytochrome b5 on these metabolisms. Japanese Journal of Veterinary Research, 45(2): 135-136. MAEDA, Yutaka (1997) Strain differences in age-associated change in Testosterone 6β-hydroxylation in Wistar and Dark Agouti rats. Japanese Journal of Veterinary Research, 45(2): 135-135. NIKAIDOU, Satoshi (1997) Effect of green tea on hepatic enzyme activities and mutagenic transformation of benzo[a]pyrene. Japanese Journal of Veterinary Research, 45(2): 134-134. OHKURA, Kaori (1997) Three-dimensional visualization of abdominal and thoracic organs of rodents by superimposing MRI multislices with a computer-graphic technique. Japanese Journal of Veterinary Research, 45(2): 133-133. UI, Masahiro (1997) Transcriptional analysis of Mareks disease virus (MDV) genes in MDV-transformed lymphoblastoid cells without activated cells. Japanese Journal of Veterinary ...
Safrole, a hepatocarcinogen, is converted by the microsomal mono-oxygenase system to a reactive intermediate which interacts with cytochrome P-450 to form a ligand complex. The formation of this complex is accompanied by loss of mono-oxygenase activity. The present study describes the interaction of the safrole reactive intermediate with microsomes from phenobarbital, 3-methylcholanthrene and safrole pretreated animals.. ...
Ethanol-inducible cytochrome P450 (P450IIE) is reported to be induced by ketosis. In the present study, the effects of a high fat diet on P450IIE induction and the relationship between ketone body concentration and P450IIE induction were studied by the following: 1) measurement of the activity of aniline hydroxylase, 2) immunoblot analysis for P450IIE protein, and 3) Northern blot analysis for P450IIE mRNA. The enzyme activities (aniline hydroxylase) in hepatic and renal microsomes were elevated about 2-3-fold by feeding with a high fat diet for 3 days. The increases in enzyme activities were also accompanied by 3-fold increases in immunoreactive P450IIE protein and its mRNA. In contrast, no differences were observed for the catalytic activities of N-alkoxyresorufin dealkylases or the amounts of immunoreactive P450IA and P450IIC, indicating a specific induction of P450IIE by high fat feeding. Furthermore, the increases in the levels of P450IIE mRNA correlated positively (r = 0.73) with plasma ...
Modern medical science has made such tremendous strides in the field of therapeutics that many older drugs, especially if potentially productive of untoward and dangerous side-effects, have been discredited and have fallen into disuse. Admittedly, many such drugs have marked therapeutic value and are deserving of further investigation and clinical trial before they are completely discarded.. Aminopyrine, by force of historical circumstances, has been such a discredited drug for many years.1, 2a, 3c, 4b, 5, 6, 7, 8 The danger of agranulocytosis resulting from its use is real and well established, but undoubtedly has been overemphasized.1, 2a, 4b, 5, 6, ...
Synonyms for amidopyrine in Free Thesaurus. Antonyms for amidopyrine. 1 synonym for amidopyrine: aminopyrine. What are synonyms for amidopyrine?
Oldenlandia diffusa (OD) and Scutellaria barbata (SB) have been used in traditional Chinese medicine for treating liver, lung and rectal tumours. We previously showed that they inhibited mutagenesis, DNA binding and metabolism of aflatoxin B1 (AFB1) and benzo(a)pyrene (BaP) bioactivated by Aroclor 1254-induced rat S9. The purpose of this study was to investigate the effects of OD and SB on the mutagenicity of AFB1 in Salmonella typhimurium TA100 using dexamethasone (DXM)-induced rat hepatic S9, on cytochrome P450-linked aminopyrine N-demethylase (APND) activity in DXM-induced hepatic microsomes and on the metabolism of AFB1 by DXM-induced S9 using high-performance liquid chromatography (HPLC). The experimental results showed that OD and SB consistently inhibited the mutagenicity of AFB1 bioactivated by either non-induced or DXM-induced S9. These effects correlated with the inhibition of cytochrome P450-linked APND activity in DXM-induced microsomes and with an inhibition of DXM-induced S9 mediated
Lee, S.-M. and Clemens, M. G. (1992), Effect of α-tocopherol on hepatic mixed function oxidases in hepatic ischemia/reperfusion. Hepatology, 15: 276-281. doi: 10.1002/hep.1840150217 ...
Download Free Full-Text of an article EFFECTS OF 3 - METHYL CHOLANTHEREN ON LIVER MICROSOMAL MIXED FUNCTION OXIDASE ACTIVITIES OF ACIPENSER PERSICUS
The N-demethylation of N,N-dimethylphenobarbital by isolated rat hepatocytes was increased severalfold by pretreatment of animals with sodium phenobarbital (75 mg/kg/day ip for 3 days). The apparent extent of induction depended on the length of the cell incubation, being 3-fold when calculated after a 5-min incubation and 5-fold when calculated after a 60-min incubation. This difference was due to the fact that metabolism by induced hepatocytes more closely approached linearity over a 60-min period. Microsomal cytochrome P-450 levels were increased 3-fold upon phenobarbital treatment; during incubations of less than 30 min duration, increased N-demethylase activity could be entirely accounted for by this increase in cytochrome P-450. When protein levels were measured directly in microsomes isolated from hepatocyte homogenates, the levels in induced hepatocytes were approximately 45% higher than control. However, phenobarbital treatment had no significant effects on total cellular protein or ...
Phenytoin is extensively bound to plasma proteins and is prone to competitive displacement. Phenytoin is metabolized by hepatic cytochrome P450 enzymes CYP2C9 and CYP2C19, and is particularly susceptible to inhibitory drug interactions because it is subject to saturable metabolism. Inhibition of metabolism may produce significant increases in circulating phenytoin concentrations and enhance the risk of drug toxicity. Monitoring of phenytoin serum levels is recommended when a drug interaction is suspected.. Phenytoin is a potent inducer of hepatic drug-metabolizing enzymes. ...
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BR-4935: cardiotonic; a non-adenosine analog adenosine1-receptor agonist with a substituted 2-thio-3,5-dicyano-4-phenyl-6-aminopyrine
Abstract Several parameters related to mono-oxygenase activity were followed in a population of chemical workers and controls. Workers exposed to toluene and xylene had a significant increase of urinary glucaric acid, that was correlated with hippuric acid excretion. On the other hand, workers exposed to pigments showed a marked increase of antipyrine half-life. A dose-related decrease of liver N-demethylase was induced in rats by the administration of a mixture of three of the pigments in use in the plant. Serum gamma-glutamyltranspeptidase was decreased in the workers exposed to pigments, but this variation was not statistically significant. The exposure to different chemicals in the workplace seemed to induce a complicated variation of mono-oxygenase levels, some enzyme being inhibited and others induced in the same group of workers. The sensitivity of these workers to toxic effects of chemicals, carcinogenic compounds and drugs seems to differ markedly from the control population. © 1982 ...
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ETHNOPHARMACOLOGICAL RELEVANCE: Shikonin, a naphthoquinone pigment abundant in the root of the Chinese herb Lithospermum erythrorhizon, has been widely used to treat inflammatory diseases for thousands of years. Whether shikonin changes drug metabolism remains unclear. AIM OF THE STUDY: We investigated whether shikonin modulates the expression of hepatic drug-metabolizing enzymes and transporters as well as the possible mechanisms of this action. MATERIALS AND METHODS: Primary hepatocytes isolated from Sprague-Dawley rats were treated with 0-2 μM shikonin and the protein and mRNA levels of drug-metabolizing enzymes and transporters as well as the activation of aryl hydrocarbon receptor (AhR) and NF-E2-related factor 2 (Nrf2) were determined ...
Rats of either sex as intact or partially hepatectomized (two-thirds liver removal) were injected s.c. daily for 7 days post-operatively with natural and synthetic estrogens, androgens or anabolic steroids, progesterone and adrenal cortical hormones
MF:C13H17N3O MW:231.29 CAS:58-15-1 EINECS:200-365-8 Apperance:White fronds crystal or crystalline powder. No smell, taste slightly bitter. Product use: Antipyretic and analgesic, used for fever and headache, joint pain, neuralgia, dysmenorrhea and...
Bardag-Gorce, F., Yuan, Q.X., Li, J., French, B.A., Fang, C., Ingelman-Sundberg, M., French, S.W., 2000. The effect of ethanol-induced cytochrome p4502E1 on the inhibition of proteasome activity by alcohol. Biochem. Biophys. Res. Commun. 279, 23-29.. Bondoc, F.Y., Bao, Z., Hu, W.Y., Gonzalez, F.J., Wang, Y., Yang, C.S., Hong, J.Y., 1999. Acetone catabolism by cytochrome P450 2E1: studies with CYP2E1-null mice. Biochem. Pharmacol. 58, 461-463.. Cederbaum, A.I., 2014. Methodology to assay CYP2E1 mixed function oxidase catalytic activity and its induction. Redox Biol. 2C, 1048-1054.. Cheng, J., Chen, C., Kristopher, K.W., Manna, S.K., Scerba, M., Friedman, F.K., Luecke, H., Idle, J.R., Gonzalez, F.J., 2013. Identification of 2-piperidone as a biomarker of CYP2E1 activity through metabolomic phenotyping. Toxicol. Sci. 135, 37-47.. Cheung, C., Gonzalez, F.J., 2008. Humanized mouse lines and their application for prediction of human drug metabolism and toxicological risk assessment. J. Pharmacol. Exp. ...
Product Name: 2,8-diiododibenzofuranFormula: C12H6OI2Weight: 419.98344SMILES: IC1=CC2C3=C(C=CC(I)=C3)OC=2C=C1CAS NO: 127-48-0 Product: Trimethadione &
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Autori: Lefter LP, Sunamura M, Furukawa T, Yastsuoka T, Abe H, Inoue H, Abe T, Egawa S, Miura K, Morita R, Horii A, Matsuno S.. Editorial: Asian J Surg. 2004 Apr;27(2):85-92., 2004.. Rezumat:. BACKGROUND: In a previous work, we demonstrated that loss of heterozygosity of 18q is a frequent event significantly associated with poor prognosis in pancreatic cancer. We hypothesized that restoration of heterozygosity of chromosome 18 in pancreatic cancer cells would reduce their tumorigenicity. This study was intended to provide functional evidence for the existence of new tumour suppressor gene(s) located on chromosome 18. METHOD: Restoration of heterozygosity was achieved by introducing a normal copy of chromosome 18 into pancreatic ductal carcinoma using a microcell-mediated chromosome transfer technique. The tumorigenicity and metastatic ability of both the parental cells and resulting hybrids were assessed in vitro and in vivo. RESULTS: In vitro growth of hybrid clones was significantly delayed ...
TY - JOUR. T1 - Hepatic Microsomal Induction in Rat Liver. T2 - Heterogeneity of Response. AU - Kiernan, Thomas W. AU - LUISADA‐OPPER, A.. AU - ERTEL, NORMAN H.. PY - 1981/1/1. Y1 - 1981/1/1. N2 - Abstract: The concept that urinary 6‐hydroxycortisol excretion patterns reflect all microsomal enzyme activities remains a subject of controversy. Three different microsomal hydroxylase activities were therefore studied in rat liver after the animals were intoxicated with alcohol, phenobarbital, or a combination thereof. The activities of pentobarbital hydroxylase, aniline hydroxylase, and cortisol 6‐hydroxylase (the enzyme metabolizing cortisol to 6‐hydroxycortisol) were assayed simultaneously after each treatment. A heterogeneity of enzyme activities was noted. Cortisol 6‐hydroxylase activity did not parallel the activity patterns of the two other hydroxylases. The data suggest that before urinary 6‐hydroxycortisol excretion patterns can be utilized as an index of microsomal enzyme ...
Earlier we demonstrated that meta-iodobenzylguanidine (MIBG), a specific inhibitor of arginine mono-ADP-ribosylation blocks proliferation and differentiation of chick skeletal myogenic cells in...
The Epigenase JMJD3/UTX Demethylase Activity/Inhibition Assay Kit (Fluorometric) is a complete set of optimized reagents, designed for measuring activity/inhibition of JMJD3 and UTX using nuclear extracts or purified enzymes...
Although the aminopyrine breath test has received much attention, the question has not yet been settled whether pharmacological or tracer doses of the drug should be used. Nine volunteers were given14...
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In toxicological research, immortalized human hepatocytes provide a useful alternative to primary hepatocytes because interindividual variability in the expression of drug-metabolizing enzymes and drug transporters can largely be eliminated. However, it is essential that the cell line retain the original phenotype. The purpose of this study was to characterize a novel spontaneously immortalized human hepatocyte cell line, HC-04, with respect to the transcript and functional protein expression profile for the major drug-metabolizing enzymes and transmembrane transporters. HC-04 cells retained hepatocyte-specific function including albumin production and ornithine transcarbamoylase and glucose-6-phosphatase activity. Most of the major CYP forms were expressed at basal levels and responsive to inducing agents. In particular, CYP3A4 was expressed abundantly, and HC-04 cells were able to metabolize the CYP3A4 probe, midazolam, at a rate similar to primary human hepatocytes. Furthermore, the major ...
DNA Demethylase Activity/Inhibition Assay Kit is use for measuring DNA demethylase activity/inhibition. (KA0677) - Products - Abnova
The effect of ethanol on N-demethylation of aminopyrine in rat liver slices and in the microsomal fraction and on microsomal hydroxylation of pentobarbital and aniline was studied. With liver slices N-demethylation of aminopyrine was stimulated by 35-40% at low ethanol concentrations (2mm), whereas no stimulation occurred at high concentrations (100mm). With the liver microsomal fraction, an inhibitory effect was observed only at high ethanol concentrations (100mm). This was also observed with the other drugs studied. In agreement with these results, only at a high concentration did ethanol interfere with the binding of drug substrates to cytochrome P-450. Further, as previously reported, ethanol produced a reverse type I spectral change when added to the liver microsomal fraction. Evidence that this spectral change is due to removal of substrate, endogenously bound to cytochrome P-450, is reported. A dual effect of ethanol is assumed to explain the present findings; in liver slices, at a low ...
dentification of the fundamental polypeptide difference between yellow (Ay/-, Avy/-) and non-yellow mice is important for biomedical research because of the influence of the yellow genotype on normal and neoplastic growth and obesity. The complexity of the yellow mouse syndrome makes attainment of this objective dependent on the separation of those pleiotropic enzyme differences which are secondary, and depend on the background genome, from those which are primary, and depend primarily on the agouti locus genotype.-Four of nine hepatic enzyme activities assayed simultaneously differed between eight-week-old yellow (Ay/-, Avy/-) and non-yellow (A/-, a/a) male inbred and F1 hybrid mice. Among these four, only cytoplasmic malic enzyme activity was elevated in all yellow mice, as compared with the non-yellow sibs, regardless of background genome. Glucokinase, serine dehydratase, and tyrosine α-ketoglutarate transaminase activities were also changed in yellow mice, but these alterations depended ...
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N-Nitrosodimethylamine is a procarcinogen that is activated by cytochrome P450 dependent N-nitrosodimethylamine N-demethylase to labile alpha-carbon hydroxylated products further resulting in active methylating agents. In vivo intraperitoneal administration of pyridine to rabbits significantly increased N-nitrosodimethylamine N-demethylase activity by 6.9- and 5.2-fold in liver and lung microsomes, respectively. Although, p-nitrophenol hydroxylase and aniline 4-hydroxylase activities were markedly enhanced by pyridine treatment in liver about 4.4- and 5.8-fold, respectively, no change was observed in the activities of these enzymes in lung microsomes. Pyridine treatment also elevated P450 contents of liver and lung by 2.04- and 1.4-fold, respectively. SDS-PAGE of pyridine-induced liver microsomes revealed a protein band of enhanced intensity having M-r of 51,000 migrating in the region of cytochrome P4502E1. The results obtained in this study demonstrated for the first time, a significant ...
Trimethadione anticonvulsant drug molecule. Used in treatment of seizures. Atoms are represented as spheres with conventional colour coding: hydrogen (white), carbon (black), oxygen (red), nitrogen (blue). - Stock Image F011/3640
TY - JOUR. T1 - The kinetics of ethylmorphine N-demethylation in isolated hepatocytes. T2 - The effect of drug transport and oxygen concentration. AU - Erickson, R. R.. AU - Yu-Drent, P.. AU - Holtzman, J. L.. PY - 1982/1/1. Y1 - 1982/1/1. N2 - In previous studies we have observed that the K(m) for ethylmorphine N-demethylase was only 50 to 100 μM in the isolated hepatocyte as opposed to 250 to 340 μM in isolated microsomes. These data suggested that either the drug is actively transported into the cell or that the enzymes in the cell had a reduced turnover number. Our current study indicates that ethylmorphine is transported into the hepatocyte with a K(m) of 250 μM and Tm of 20 nmol/min 106 cells. On the basis of temperature and inhibitor studies, this would appear to be active transport. When the turnover number of the ethylmorphine N-demethylase was decreased by reducing the oxygen from 20% of the gas phase to 5%, the activity was partially uncompetitively inhibited with the K(m) ...
Rph1 and Gis1 are two related yeast zinc finger proteins that function as downstream effectors in the Ras/PKA, TOR and Sch9 nutrient signaling pathways. Both proteins also contain JmjC histone demethylase domains, but only Rph1 is known to be an active enzyme, demethylating lysine 36 of histone H3. We have studied to what extent the demethylase activity of Rph1 contributes to its role in nutrient signaling by performing gene expression microarray experiments on a yeast strain containing a catalytically inactive allele of RPH1. We find that the enzymatic activity of Rph1 is not essential for its role in growth phase dependent gene regulation. However, the ability of Rph1 to both activate and repress transcription is partially impaired in the active site mutant, indicating that the demethylase activity may enhance its function in vivo. Consistent with this, we find that the Rph1 mutation and a deletion of the histone H3 methylase Set2 affect the same target genes in opposite directions. Genes that ...
With that encouragement Axelrod quickly showed that amphetamine was rapidly metabolized in rabbit liver slices. He went on to define the co-factor requirements for the reaction finding that NADPH was required; similar to observations of Bert LaDu in his studies on aminopyrine demethylation9.Axelrod then determined the sub-cellular location of the activity by using methods developed by Hogeboom and Schneider10. This newly defined microsomal oxidizing system was soon shown to be responsible for the metabolism of a wide variety of drugs and other chemicals.. Ref: 1V.R.Potter and C.A.Elvehjem, J.Biol. Chem. ,114:495-504 (1936) 2A.Claude, Cold String Harbor Symposium Quant.Biol.,9:263-270 (1941)3 W.C.Schneider,J.Biol.Chem. 165:585-593 (1949) 4E.C.Miller and J.A.Miller, Cancer Res. 7:468-480 (1947)5 E.S.Stevenson, K. Dobriner, and C.P.Rhoads, Cancer Research 2:160-167 (1942)6 G.C.Mueller and J.A.Miller, J.Biol.. Chem. 176:535-544 (1948) 7G.C.Mueller and J.A. Miller, J.Biol. Chem. ,180:1125-1236 ...
Algorithm for the management of hepatic enzyme elevations in the INPULSIS® trials. *Defined as increase in hepatic transaminases (AST or ALT) to ≥3 x ULN, an
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Hondas NSX hero car is to make its fabled return to the supercar market after all, according to reports out of the Shanghai Auto Show this week.. Earlier reports claimed the new NSX would be downgraded from supercar to six-cylinder sports car, built on a reversed version of the Accord platform.. According to US industry paper Autonews however, Honda CEO Takanobu Ito has confirmed that a spiritual successor to the NSX is in development.. Dont expect a big Lexus LFA-beating V10 engine however: no details have been confirmed, but Honda is understood to have ruled out the big engine approach, going instead for an environmentally-friendly hybrid drivetrain.. Thats the kind of sports car we want to make, Ito reportedly told Autonews at the Shanghai show this week, describing a car that would be exhilirating to drive and environmentally-friendly.. Dropping the planned V10 heart in favour of a hybrid approach suggests Honda is watching closely BMWs Vision EfficientDynamics-based hybrid ...
Quantification of highly homologous human liver drug-metabolizing enzymes (DMEs) has been a challenging task in drug metabolism and disposition research, due to a lack of specific antibodies and marker substrates. Mass spectrometry (MS) techniques and applications have evolved significantly, striving to achieve absolute and specific quantification of these enzymes. Since the first absolute quantification of cytochrome P450s (CYPs) using the attachment of isotope tags to free thiols (iCAT), MS-based quantification has become much more versatile, cheaper, and easier to use. Today, variations of liquid chromatography-multiple reaction monitoring (LC-MRM)-based targeted proteomics, such as AQUA (absolute quantification) and QconCAT (concatenated signature peptides), have become the gold standards for quantification. These new methods have driven the absolute quantification of DMEs to become a routine laboratory task. Many drug metabolism-related projects require absolute enzyme quantification. For ...
Primary treatment-related effects included: initial sedation of animals exposed to 3,000 ppm and its subsequent resolution correlating with induction of hepatic mixed function oxidase activity and S-phase DNA synthesis; elevated mortality in high-exposure male rats and mice (chronic study); elevated deposition of alpha2u-globulin (alpha2U-G) and associated nephropathy and S-phase DNA synthesis in male rat kidneys; accelerated atrophy of the adrenal gland X-zone in female mice (subchronic study only); and increased occurrence and/or severity of eosinophilic foci of altered hepatocytes in male rats ...
Oral The metabolism of nonyl phenol ethoxylates takes place by shortening the ethylene oxide chain, carboxylation of the alkyl chain by omega oxidation and subsequent glucoronide and sulphate conjugation (CIR, 1983). Knaak et al. (1966) fed 67 mg/kg of NP-14C TP-9 (a commercial product containing on average 9 moles of EO per mole of NP, i.e. NPE-9) or 14C-NP to rats and followed urinary, faecal over a period of 7 days and CO2 excretion over a period of 4 days. Within the observed time period, 20% of the NP-14C TP-9 was excreted in urine, 78% in faeces and none as14CO2 14C-NP per se was found to be excreted in a similar manner. In the same study, Knaak et al. also exposed rats to 7, 10, 12 and 15 mole adducts of NP isolated from ethylene oxide-labelled TP-9. The 12 and 15 mole adducts were excreted to a greater extent in the feces than the 7 and 10 mole adducts, while the reverse situation occurred in urine. Modelling the metabolism of NPE-2 and NPE-4 in liver using the using prediction tools ...
Hasegawa R, St John MK, Cano M, Issenburg P, Klein DA, Walker BA, Jones JW, Schnell RC, Merrick BA, Davies MH, McMillan DA, Cohen SM. 1984. Bladder freeze ulceration and sodium saccharin feeding in the rat, examination for urinary nitrosamines, mutagens, and bacteria, and effects on hepatic microsomal enzymes. Food Chem Toxicol 22:935-942.. View Abstract ...
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"Aminopyrine-N-demethylase. I. Directed modification of substrates' structure as a way of production of inducer of the ...
... aminopyrine n-demethylase MeSH D08.811.682.662.582.338 - cytochrome p-450 cyp2e1 MeSH D08.811.682.662.582.353 - cytochrome p- ... nitroanisole o-demethylase MeSH D08.811.682.690.416 - dioxygenases MeSH D08.811.682.690.416.277 - catechol 1,2-dioxygenase MeSH ... ethylmorphine-n-demethylase MeSH D08.811.682.662.582.550 - sarcosine dehydrogenase MeSH D08.811.682.662.582.700 - sarcosine ...
Imaoka S, Inoue K, Funae Y (1988). "Aminopyrine metabolism by multiple forms of cytochrome P-450 from rat liver microsomes: ... Suhara K, Ohashi K, Takahashi K, Katagiri M (1988). "Aromatase and nonaromatizing 10-demethylase activity of adrenal cortex ... simultaneous quantitation of four aminopyrine metabolites by high-performance liquid chromatography". Arch. Biochem. Biophys. ...
Imaoka, S., Inoue, K. and Funae, Y. (1988). "Aminopyrine metabolism by multiple forms of cytochrome P-450 from rat liver ... Suhara, K., Ohashi, K., Takahashi, K. and Katagiri, M. (1988). "Aromatase and nonaromatizing 10-demethylase activity of adrenal ... microsomes: simultaneous quantitation of four aminopyrine metabolites by high-performance liquid chromatography". Arch. Biochem ...
In contrast to the biphasic kinetic behavior of hepatic aminopyrine N-demethylase, brain aminopyrine N-demethylation exhibited ... In contrast to the biphasic kinetic behavior of hepatic aminopyrine N-demethylase, brain aminopyrine N-demethylation exhibited ... In contrast to the biphasic kinetic behavior of hepatic aminopyrine N-demethylase, brain aminopyrine N-demethylation exhibited ... In contrast to the biphasic kinetic behavior of hepatic aminopyrine N-demethylase, brain aminopyrine N-demethylation exhibited ...
In rats treated with a single dose [50 mg/kg] of SKF 525-A, aminopyrine N-demethylase and aniline hydroxylase activities were ... M, in single and multiple doses on hypatic microsomal aminopyrine n-demethylase and aniline hydroxylase activities in the rat: ... M, in single and multiple doses on hypatic microsomal aminopyrine n-demethylase and anilin ... The effects of diclofenac sodium administration, in single and repeated doses, on hepatic microsomal activities of aminopyrine ...
"Aminopyrine-N-demethylase. I. Directed modification of substrates structure as a way of production of inducer of the ...
... aminopyrine N-demethylase; AUC0-24, area under concentration-time curve from zero to 24 h. ...
Liver aminopyrine N-demethylase activity was increased in all permethrin-treated animals. Bone marrow smears of the animals ... Hepatic aminopyrine N-demethylase activity was also substan- tially increased, although not consistently, in both male and ... increased liver aminopyrine- N-demethylase ac- tivity, increased liver weight, and eosinophilia of hepatocytes in both males ... increased liver aminopyrine- N-demethylase activity in both males and females at 1000 and 2500 mg/kg, and hepatocyte ...
DEVELOPMENT OF HEPATIC N-DEMETHYLASE ACTIVITY AS MEASURED IN VIVO BY THE AMINOPYRINE BREATH TEST *Robert J Shulman ... Rights & permissionsfor article DEVELOPMENT OF HEPATIC N-DEMETHYLASE ACTIVITY AS MEASURED IN VIVO BY THE AMINOPYRINE BREATH ... Rights & permissionsfor article Effect of Infant Age on Aminopyrine Breath Test Results . Opens in a new window. ... Effect of Infant Age on Aminopyrine Breath Test Results *Robert J Shulman ...
NKK-105 had no effect on the activity of aminopyrine N-demethylase and the content of cyt.b5, cyt.p-450 and peroxide. The ... Effects of NKK-105 administration on aminopyrine N-demethylase, cyt.b5, cyt.p-45O and lipid peroxidation in CCl4-induced liver ... Aminopyrine N-demethylase activity and cyt. P-450 content were not affected under the same experimental conditions. However, ... p-450 and the demethylase activity were restored about 75% of the control values and cyt.b5 content was completely restored ...
The effects on aminopyrine-demethylase and acetanilide-hydroxylase activity were evaluated. The extracts were examined by thin ... Isooctane did not inhibit aminopyrine-demethylase or acetanilide-hydroxylase activity. TLC spots corresponding to ...
The activity of aminopyrine demethylase rose by a factor of 2.2 and the mean liver weights increased by 35%. There was a ... aminopyrine demethylase activities, for levels of cytochromes P-450 and b5, and for microsomal protein concentration. At 27,000 ...
Aminopyrine N-demethylase (APDM) activity and electron microscopy on liver samples (6 males and 6 females/group). Statistics:. ... A statistically significant increase in liver aminopyrine N-demethylase activity was seen in animals of both sexes given the ... A statistically significant increase in liver aminopyrine N-demethylase activity was seen in animals of both sexes given the ... Aminopyrine N-demethylase and electron microscopy).. Executive summary:. Sprague Dawley CD rats of received diets containing ...
The effects on aniline-p-hydroxylase, acetanilide-hydroxylase, and aminopyrine-N- demethylase activity were investigated. IPI ... hydroxylase and acetanilide-hydroxylase and noncompetitively inhibited aminopyrine-N-demethylase, IPI being the more potent. ...
Pretreatment with momordin Ic and oleanolic acid resulted in significantly lower production of aminopyrine N-demethylase and ... Pretreatment with momordin Ic resulted in lower catalase and aminopyrine N-demethylase activity induction by CCl4, towards ...
1973) Dimethylamine N-oxidase and aminopyrine N-demethylase activities in human liver tissues. Xenobiotica 3:179-189. ...
Nickel carbonyl inhibition of induction of aminopyrine demethylase activity in liver and lung. Cancer Res 1970;30:1645-50. ... Noda A, Tsubone N, Mihara M, Goromaru T, Iguchi S. Formation of 4-formylaminoantipyrine as a new metabolite of aminopyrine. II ... Mirvish SS, Gold B, Eagen M, Arnold S. Kinetics of the nitrosation of aminopyrine to give dimethylnitrosamine. Clin Oncol 1974; ... Bast A, Noordhoek J. Inhibition of aminopyrine demethylation and binding to cytochrome P-450 by its main metabolites in rat ...
Competitive inhibition was followed by Lineweaver-Burke analyses of aminopyrine demethylase in operated females treated with ... aminopyrine demethylase and aromatic hydrocarbon or benzo[a]pyrene hydroxylase). Groups were also induced with phenobarbital ... P-450 and the other steroids screened had little effect on the microsomal parameters except for a rise in demethylase with ...
Critical role of lipid peroxidation in carbon tetrachloride-induced loss of aminopyrine demethylase, cytochrome P-450 and ...
Characterization of cytochrome P-450-dependent aminopyrine N-demethylase in rat brain: comparison with hepatic aminopyrine N- ...
CHLORINATED PARAFFINS 463 females; females: increased aminopyrine N-demethylase activity, N- acetylglucosaminidase activity, ... CHLORINATED PARAFFINS 446 pyrine N-demethylase activities were increased in male and female Sprague-Dawley rats fed 5,000 ppm ...
Results: The activities of benzphetamine and aminopyrine Ar-demethylase were increased in the cultural fetal adrenal cells ... The activities of 7-ethoxyresorufin 0-dealkylase, benzphetamine, aminopyrine and erythromycin N-demethylases were measured by ... Aryl hydrocarbon hydroxylase (AHH) and dimethylnitrosamine demethylase (DMND) activities in pulmonary and hepatic tissues of ... also increased the activity of erythromycin W-demethylase. The activity of 7-ethoxyresorufin 0-dealkylase was undetected in the ...
... and aminopyrine demethylase activity (2.59 ± 0.08 and 3.48 ± 0.13 µM/g liver/5 min formaldehyde production) checked.. Test ...
... in vitro activities of aniline hydroxylase and aminopyrine demethylase, content of cytochromes P-450 and b5/mg microsomal ...
Benzofuran administration also caused decreases in cytochrome P-450 levels and aminopyrine N-demethylase activities. ...
... aminopyrine n-demethylase MeSH D08.811.682.662.582.338 - cytochrome p-450 cyp2e1 MeSH D08.811.682.662.582.353 - cytochrome p- ... nitroanisole o-demethylase MeSH D08.811.682.690.416 - dioxygenases MeSH D08.811.682.690.416.277 - catechol 1,2-dioxygenase MeSH ... ethylmorphine-n-demethylase MeSH D08.811.682.662.582.550 - sarcosine dehydrogenase MeSH D08.811.682.662.582.700 - sarcosine ...
3) The activity of aminopyrine demethylase, the contents of cytochrome P-450 and b5, and the spectral difference of aniline ...
Aminopyrine N-demethylase activities in microsomes from AMDB-treated rats were not well correlated with cytochrome P-450 levels ...
... including cytochrome P-450 and aminopyrine- N- demethylase; cytochrome- c-reductase activity was decreased, and those of ... demethylase, and cytochrome- c-reductase. The activities of the phase-II drug metabolizing enzymes glucuronyl transferase I and ... demethylase, benzo[ a]pyrene hydroxylase, 7-ethoxycoumarin-deethylase, and cytosolic glutathione- S-transferase were measured. ...
Under the same experimental condition, the amounts of cytochrome P-450 and b5, the activities of aminopyrine N-demethylase, ... The effect of a 20-day administration of aminopyrine (600 mg kg-1) as well as two metabolites of aminopyrine, 4-aminoantipyrine ... Induction of hepatic gamma-glutamyl transpeptidase in rats by repeated administration of aminopyrine.. T Satoh, T Igarashi, T ... Induction of hepatic gamma-glutamyl transpeptidase in rats by repeated administration of aminopyrine.. T Satoh, T Igarashi, T ...
... aminopyrine N-demethylase, aryl hydrocarbon hydroxylase, aniline hydroxylase,and NADPH-cytochrome c reductase in the rodent ...
... glucose-6-phosphatase and aminopyrine N-demethylase were determined. Oral administration at 600 mg/kg per day, for 3 days ... glucose-6-phosphatase and aminopyrine N-demethylase were determined. Oral administration at 600 mg/kg per day, for 3 days ... glucose-6-phosphatase and aminopyrine Ndemethylase were determined. Statistics:. 1. All statistical analyses were performed ...
  • The effects of diclofenac sodium administration , in single and repeated doses , on hepatic microsomal activities of aminopyrine N- demethylase and aniline hydroxylase were evaluated in rats . (bvsalud.org)
  • In rats treated with a single dose [50 mg/kg] of SKF 525-A, aminopyrine N-demethylase and aniline hydroxylase activities were significantly decreased, compared with control values. (bvsalud.org)
  • The effects on aminopyrine-demethylase and acetanilide-hydroxylase activity were evaluated. (cdc.gov)
  • Isooctane did not inhibit aminopyrine-demethylase or acetanilide-hydroxylase activity. (cdc.gov)
  • The effects on aniline-p-hydroxylase, acetanilide-hydroxylase, and aminopyrine-N- demethylase activity were investigated. (cdc.gov)
  • IPI and CPI competitively inhibited aniline-p- hydroxylase and acetanilide-hydroxylase and noncompetitively inhibited aminopyrine-N-demethylase, IPI being the more potent. (cdc.gov)
  • Pretreatment with momordin Ic and oleanolic acid resulted in significantly lower production of aminopyrine N-demethylase and aniline hydroxylase in the CCl4-treated rats. (biomedsearch.com)
  • Rats of either sex as intact or partially hepatectomized (two-thirds liver removal) were injected s.c. daily for 7 days post-operatively with natural and synthetic estrogens, androgens or anabolic steroids, progesterone and adrenal cortical hormones and killed on day 10 at which time the livers were processed for microsomal analyses (protein, cytochrome P-450 and enzymes, aminopyrine demethylase and aromatic hydrocarbon or benzo[a]pyrene hydroxylase). (biomedsearch.com)
  • Also following dietary administration, hepatic microsomes were isolated and the following parameters related to in vitro drug metabolism were measured, yield of microsomal protein/g liver, in vitro activities of aniline hydroxylase and aminopyrine demethylase, content of cytochromes P-450 and b5/mg microsomal protein. (epa.gov)
  • Under the same experimental condition, the amounts of cytochrome P-450 and b5, the activities of aminopyrine N-demethylase, aniline hydroxylase and carboxylesterase of liver microsomes were all induced in the aminopyrine- and 4-aminoantipyrine-treated rats. (aspetjournals.org)
  • Similarly, in a parallel group of rats that did not receive DMBA, the activities of aniline hydroxylase, aminopyrine N -demethylase, and cytochrome c reductase were not significantly altered by dietary selenium levels. (aacrjournals.org)
  • On stipulated day the animals were slaughtered, their livers taken out and microsomes prepared for the estimation of activities of various membrane bound DME such as cytochrome P450 (CP), cytochrome b5 (CB), cytochrome-c-reductase (CCR), aniline hydroxylase (AH), acetanilide hydroxylase (AAH), benzphetamine demethylase (BD), aminopyrine demethylase (APD), N. (thefreedictionary.com)
  • Administration of Zineb at doses from 200 to 600 mg/kg caused a significant impairment of aminopyrine-N-demethylase in the two species, as well as an inhibition of aniline hydroxylase in rats and a slight increase of this enzymatic activity in mice. (springer.com)
  • The enzymes investigated were aminopyrine N-demethylase, aniline 4-hydroxylase and UDP-glucuronyltransferase. (termsreign.ga)
  • 1. Benzo[a]pyrene hydroxylase, aminopyrine demethylase and glutathione S-trans-ferase activities in hepatic and extrahepatic tissues of five wild birds, rat and mouse were compared. (ias.ac.in)
  • PSK had no influence on aminopyrine N-demethylase and aniline hydroxylase activities, cytochrome P-450 concentration in hepatic microsomes, and the reductase activity of cytochrome c in normal mice. (mushroomstudies.co)
  • In the present study the toxic effects of TCE inhalation on pulmonary and hepatic biotransformation enzymes in rats have been investigated by assay of aniline hydroxylase (AH), aminopyrine-N-demethylase (APD), benzo-a-pyrene hydroxylase (BH) and glutathione-s-transferase (GST) activities and glutathione (GSH) contents in liver as well as lungs of exposed animals. (who.int)
  • Then, aminopyrine N-demethylase, aniline 4-hydroxylase, aromatase, caffeine N-demethylase, cytochrome b5 reductase, erythromycin N-demethylase, ethoxyresorufin O-deethylase, glutathione S-transferase, N-nitrosodimethylamine N-demethylase and penthoxyresorufin O-deethylase activities were determined to follow changes in the activity of drug metabolizing enzymes in SOX-deficient rats. (agu.edu.tr)
  • As shown in Table 1, [gamma]-HCH treatment resulted in a significant increase in hepatic microsomal NADPH cytochrome Creductase by 71%, while [gamma]-HCH decreased both amidopyrine N-demethylase and aniline-4-hydroxylase by 11 and 27%, respectively when compared to control. (freethesaurus.com)
  • 450s] (except hepatic amidopyrine N-demethylase and aniline 4-hydroxylase activities). (freethesaurus.com)
  • A significant inhibition of hepatic microsomal amidopyrine N-demethylase and aniline 4-hydroxylase activities observed in [gamma]-HCH intoxicated animals compared to control. (freethesaurus.com)
  • Both compounds increased the aminopyrine-N-demethylase activity, decreased the glucose 6-phosphatase (G6Pase), alkaline phosphodiesterase I and alkaline phosphatase specific activities, but did not modify the γ-glutamyltransferase levels. (elsevier.com)
  • Daily administration of cannabis extract for 15 consecutive days increased the aminopyrine-N-demethylase activity which was significant on day 15 post-treatment at 2 and 10 mg/kg doses. (bvsalud.org)
  • Glende EA, Hruszkewycz AM, Recknagel RO (1976) Critical role of lipid peroxidation in carbon tetrachloride-induced loss of aminopyrine demethylase, cytochrome P-450 and glucose-6-phosphatase. (springer.com)
  • Significant decreases in the level of liver microsomal cytochrome P-450 (67%), heme (37%), aminopyrine N-demethylase (60%) and glucose-6-phosphatase (58%), were noticed 24 hr after pulegone treatment. (iisc.ernet.in)
  • As a result of these alterations, the hepatic microsomal MFO system was imparied as evidenced by a decrease in cytochrome P-450 system content and in the activities of NADPH-cytochrome C reductase and aminopyrine demethylase. (who.int)
  • Further studies found that the liver microsomal cytochrome P-450 content and aminopyrine demethylase activity were significantly increased in mice treated with SY-640 (150 mg.kg-1 p.o.) once daily for three days, while the hepatic microsomal aminopyrine demethylase activity was obviously inhibited two hours after oral administration of SY-640 150 mg.kg-1 in mice. (statescale.tk)
  • This study was designed to characterize cytochrome P-450-dependent aminopyrine N-demethylation using microsomes from rat brain and to compare it with N-demethylation of the drug by hepatic microsomes studied in parallel. (elsevier.com)
  • Aminopyrine N-demethylase of brain microsomes required oxygen and NADPH for optimal activity and its activity was inhibited by carbon monoxide (CO) and SKF 525-A in a concentration-dependent manner. (elsevier.com)
  • Aminopyrine N-demethylase activities in microsomes from AMDB-treated rats were not well correlated with cytochrome P-450 levels or spectral complex formation. (aspetjournals.org)
  • In the aminopyrine-treated group, a pronounced induction of gamma-glutamyl transpeptidase was shown in the whole homogenate as compared to that in the hepatic microsomes. (aspetjournals.org)
  • Maximal aminopyrine N-demethylase activity of the brain was 0.5 and 17% that of the liver when expressed per milligram of microsomal protein (261.3 pmol/10 min/mg of protein) and nanomole of cytochrome P-450 (6.98 nmol/10 min/nmol of cytochrome P-450), respectively. (elsevier.com)
  • NKK-105 had no effect on the activity of aminopyrine N-demethylase and the content of cyt.b5, cyt.p-450 and peroxide. (go.jp)
  • The content of cyt.b5, cyt.p-450 and the activity of demethylase remarkably decreased with CCl 4 administration, but the content of cyt. (go.jp)
  • p-450 and the demethylase activity were restored about 75% of the control values and cyt.b5 content was completely restored with previous administration of NKK-105. (go.jp)
  • Pretreatment with momordin Ic resulted in lower catalase and aminopyrine N-demethylase activity induction by CCl4, towards normalization. (biomedsearch.com)
  • protein content (28.4 ± 0.37 and 24.2 ± 4.19 mg/mL) and aminopyrine demethylase activity (2.59 ± 0.08 and 3.48 ± 0.13 µM/g liver/5 min formaldehyde production) checked. (europa.eu)
  • Serum gamma-glutamyl transpeptidase activity was also increased by administration of aminopyrine or 4-aminoantipyrine. (aspetjournals.org)
  • The results in this paper support the view that repeated administration of aminopyrine induces hepatic membrane-bound enzyme, particularly, gamma-glutamyl transpeptidase activity. (aspetjournals.org)
  • This difference in the degree of hepatic responsiveness, however, varied with the substrates: ethylmorphine N-demethylase activity was affected the most whereas aminopyrine N-demethylase and hexobarbital oxidase activities were only marginally affected. (aspetjournals.org)
  • Furthermore, these differences in responsiveness seem to be a delayed event, since hepatic aminopyrine N-demethylase activity in rats castrated at birth did respond to androgen stimulation during the prepubertal period and became insensitive to this same androgen stimulation at adulthood. (aspetjournals.org)
  • The effect of cannabis extract, on the hepatic aminopyrine-N-demethylase activity was studied in rats. (bvsalud.org)
  • These findings suggest that cannabis extract can induce hepatic aminopyrine-N-demethylase activity. (bvsalud.org)
  • Recently we found that the CYP3A aminopyrine N-demethylase (AMND) activity of hepatocytes cultured on collagen surface oscillated with culture time. (elsevier.com)
  • The activity of CYP2H and CYP3A37, enzymes involved in biotransformation in chicken, was detected by aminopyrine N-demethylation and aniline-hydroxylation assays from the microsomal suspensions. (biomedcentral.com)
  • At the end of the 3-month toxicity study, liver Aminopyrine N-demethylase (APDM) activity was shown to be increased in males and females at 2000 ppm only, indicating clearly induced metabolism, which might be responsible for the non-dose proportionality of the plasma levels. (europa.eu)
  • On the other hand, P. acnes administration significantly decreased the amount of cytochromes P-450 and b5 and aminopyrine N-demethylase activity. (mushroomstudies.co)
  • Additionally, DEX induced CYP3A activity (induction of aminopyrine N-demethylase and erythromycin N-demethylase) in two grass carp cell lines (Li et al. (luteinizing-hormone-releasing-hormone-human-acetate-salt.com)
  • The effect of T. crispa on aminopyrine N-demethylase activity was determined in the absence or presence of inhibitors by measuring the quantity of formaldehyde formed using the method of Nash [2]. (myjurnal.my)
  • Our results clearly demonstrated that SOX deficiency significantly elevated A4H, caffeine N-demethylase, erythromycin N-demethylase and N-nitrosodimethylamine N-demethylase activities while decreasing ethoxyresorufin O-deethylase and aromatase activities. (agu.edu.tr)
  • Rats with their androgen deprived during the neonatal period (female rats and male rats castrated at birth) respond less to androgen treatment at adulthood for the metabolism of aminopyrine, ethylmorphine and hexobarbital as compared to rats exposed to neonatal androgen (male rats, male rats castrated at birth but neonatally treated with androgen and male rats castrated at the age of 20 days). (aspetjournals.org)
  • As demonstrated by increased aminopyrine N-demethylase (APDM) activities at 2000 ppm, metabolism was considered to be stimulated. (europa.eu)
  • The objective of this study is to elucidate the effects of T. crispa on the molecular mechanism of aminopyrine metabolism in rat hepatocytes. (myjurnal.my)
  • No significant changes in P-450 content or in the following activities were noted: ethylmorphine- N - demethylase, lauric acid 1 1-hydroxylation, and lauric acid 12-hydroxylation. (cdc.gov)
  • The effect of neonatal androgen on the apparent Michaelis constant (Km) of ethylmorphine N-demethylase was also studied in the adult rat. (aspetjournals.org)
  • Joly,J-G, Ishii, H.,Teschke, R., Hasumura, Y., Lieber, C.S.: Effect of Chronic Ethanol Feeding on the Activities and Submicrosomal Distribution of Reduced Nicotinamide Adenine Dinucleotide Phosphate-Cytochrome P-450 Reductase and the Demethylases for Aminopyrine and Ethylmorphine. (springer.com)
  • Spectral binding of aminopyrine, hexobarbital, androstenedione and aniline to rat brain cytochrome P-450 could not be detected, apparently because of the low concentration of the hemoprotein. (elsevier.com)
  • Ethoxycoumarin O-deethylase (ECOD) and aminopyrine N-demethylase (APND) rates in these 7 species were 0.23-2.1 nmol/min/mg and 0.5-11 nmol/min/mg, respectively, similar to rates in many temperate fish species. (elsevier.com)
  • Induction of hepatic gamma-glutamyl transpeptidase in rats by repeated administration of aminopyrine. (aspetjournals.org)
  • However, the enzyme was less sensitive to the inhibitory effects of CO and SKF 525-A than the hepatic N-demethylase. (elsevier.com)
  • Coupled with data presented on the inhibitory effects of CO and SKF 525-A on brain and hepatic aminopyrine N-demethylases, the kinetic data suggest that brain aminopyrine N-demethylase exhibits behavior different from that of the liver enzyme. (elsevier.com)
  • The effect of a 20-day administration of aminopyrine (600 mg kg-1) as well as two metabolites of aminopyrine, 4-aminoantipyrine (525 mg kg-1) and 4-acetamidoantipyrine (635 mg kg-1), on several hepatic, kidney and serum enzyme activities were investigated. (aspetjournals.org)
  • The effects of individual pyrrolizidine alkaloids on the mixed-function oxidase (MFO) enzyme aminopyrine N-demethylase were determined in rat liver 10000 × g supernatant. (naver.com)
  • no significant changes were noted in liver aminopyrine - N - demethylase or aniline hydrolase activities. (cdc.gov)
  • Benzofuran administration also caused decreases in cytochrome P-450 levels and aminopyrine N-demethylase activities. (surrey.ac.uk)
  • Hepatic aminopyrine demethylase and GSH S-transferase activities of wild birds were lower than those of rodents. (ias.ac.in)
  • Brain aminopyrine N-demethylation was linear with respect to protein concentrations and incubation times of up to 15 min. (elsevier.com)
  • In contrast to the biphasic kinetic behavior of hepatic aminopyrine N-demethylase, brain aminopyrine N-demethylation exhibited linear kinetics. (elsevier.com)
  • 4-Aminoantipyrine is used as a reagent for glucose determination in the presence of peroxidase and phenol, it is a metabolite of aminopyrine with analgesic, anti-inflammatory and antipyretic properties. (csnpharm.com)
  • Effects of NKK-105 administration on aminopyrine N-demethylase, cyt.b 5 , cyt.p-45O and lipid peroxidation in CCl 4 -induced liver damage were investigated, in vivo. (go.jp)
  • Competitive inhibition was followed by Lineweaver-Burke analyses of aminopyrine demethylase in operated females treated with several of the steroids. (biomedsearch.com)
  • We have characterized the behavior under tension of the native state (N), and that of the ensemble of partially folded (PF) conformations the protein visits en route to N, which collectively act as a long-lived state controlling the slow kinetic phase of the folding process. (termsreign.gq)
  • Many histone demethylases generally function through an oxidoreductive mechanism. (wakehealth.edu)
  • The single apparent Km of the brain aminopyrine N-demethylase (3.39 mM) was at least 3-fold higher than that of the liver (0.18 and 1.13 mM). (elsevier.com)
  • Estradiol benzoate (15 micrograms/rat daily) depressed cytochrome P-450 and the other steroids screened had little effect on the microsomal parameters except for a rise in demethylase with deoxycorticosterone acetate (1.0 mg). (biomedsearch.com)
  • This graph shows the total number of publications written about "Histone Demethylases" by people in this website by year, and whether "Histone Demethylases" was a major or minor topic of these publications. (wakehealth.edu)