An NADPH-dependent P450 enzyme that plays an essential role in the sterol biosynthetic pathway by catalyzing the demethylation of 14-methyl sterols such as lanosterol. The enzyme acts via the repeated hydroxylation of the 14-methyl group, resulting in its stepwise conversion into an alcohol, an aldehyde and then a carboxylate, which is removed as formic acid. Sterol 14-demethylase is an unusual cytochrome P450 enzyme in that it is found in a broad variety of organisms including ANIMALS; PLANTS; FUNGI; and protozoa.
Jumonji Domain-Containing Histone Demethylases
Parietal Cells, Gastric
Retinoblastoma-Binding Protein 2
Cytochrome P-450 Enzyme System
A superfamily of hundreds of closely related HEMEPROTEINS found throughout the phylogenetic spectrum, from animals, plants, fungi, to bacteria. They include numerous complex monooxygenases (MIXED FUNCTION OXYGENASES). In animals, these P-450 enzymes serve two major functions: (1) biosynthesis of steroids, fatty acids, and bile acids; (2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs (BIOTRANSFORMATION). They are classified, according to their sequence similarities rather than functions, into CYP gene families (>40% homology) and subfamilies (>59% homology). For example, enzymes from the CYP1, CYP2, and CYP3 gene families are responsible for most drug metabolism.
A group of compounds that contain a bivalent O-O group, i.e., the oxygen atoms are univalent. They can either be inorganic or organic in nature. Such compounds release atomic (nascent) oxygen readily. Thus they are strong oxidizing agents and fire hazards when in contact with combustible materials, especially under high-temperature conditions. The chief industrial uses of peroxides are as oxidizing agents, bleaching agents, and initiators of polymerization. (From Hawley's Condensed Chemical Dictionary, 11th ed)
Artifactual vesicles formed from the endoplasmic reticulum when cells are disrupted. They are isolated by differential centrifugation and are composed of three structural features: rough vesicles, smooth vesicles, and ribosomes. Numerous enzyme activities are associated with the microsomal fraction. (Glick, Glossary of Biochemistry and Molecular Biology, 1990; from Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Mixed Function Oxygenases
Widely distributed enzymes that carry out oxidation-reduction reactions in which one atom of the oxygen molecule is incorporated into the organic substrate; the other oxygen atom is reduced and combined with hydrogen ions to form water. They are also known as monooxygenases or hydroxylases. These reactions require two substrates as reductants for each of the two oxygen atoms. There are different classes of monooxygenases depending on the type of hydrogen-providing cosubstrate (COENZYMES) required in the mixed-function oxidation.
Lining of the STOMACH, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. The surface cells produce MUCUS that protects the stomach from attack by digestive acid and enzymes. When the epithelium invaginates into the LAMINA PROPRIA at various region of the stomach (CARDIA; GASTRIC FUNDUS; and PYLORUS), different tubular gastric glands are formed. These glands consist of cells that secrete mucus, enzymes, HYDROCHLORIC ACID, or hormones.
A genetic process by which the adult organism is realized via mechanisms that lead to the restriction in the possible fates of cells, eventually leading to their differentiated state. Mechanisms involved cause heritable changes to cells without changes to DNA sequence such as DNA METHYLATION; HISTONE modification; DNA REPLICATION TIMING; NUCLEOSOME positioning; and heterochromatization which result in selective gene expression or repression.
Rats, Inbred Strains
A family of proteins that share the F-BOX MOTIF and are involved in protein-protein interactions. They play an important role in process of protein ubiquition by associating with a variety of substrates and then associating into SCF UBIQUITIN LIGASE complexes. They are held in the ubiquitin-ligase complex via binding to SKP DOMAIN PROTEINS.
The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)
A propylamine formed from the cyclization of the side chain of amphetamine. This monoamine oxidase inhibitor is effective in the treatment of major depression, dysthymic disorder, and atypical depression. It also is useful in panic and phobic disorders. (From AMA Drug Evaluations Annual, 1994, p311)
A megaloblastic anemia occurring in children but more commonly in later life, characterized by histamine-fast achlorhydria, in which the laboratory and clinical manifestations are based on malabsorption of vitamin B 12 due to a failure of the gastric mucosa to secrete adequate and potent intrinsic factor. (Dorland, 27th ed)
Peroxides produced in the presence of a free radical by the oxidation of unsaturated fatty acids in the cell in the presence of molecular oxygen. The formation of lipid peroxides results in the destruction of the original lipid leading to the loss of integrity of the membranes. They therefore cause a variety of toxic effects in vivo and their formation is considered a pathological process in biological systems. Their formation can be inhibited by antioxidants, such as vitamin E, structural separation or low oxygen tension.
Role of C-5 chiral center in R-(+)-pulegone-mediated hepatotoxicity: metabolic disposition and toxicity of 5, 5-dimethyl-2-(1-Methylethylidene)-cyclohexanone in rats. (1/55)Metabolic disposition of 5, 5-dimethyl-2-(1-methylethylidene)-cyclohexanone (I) was examined in rats. Compound (I) was administered orally (250 mg/kg of body weight/day) to rats for 5 days. The following urinary metabolites were isolated and identified: 4,5,6,7-tetrahydro-3,6, 6-trimethylbenzofuran (III), 3,3-dimethylcyclohexanone (VI), 5, 5-dimethyl-3-hydroxy-2-(1-methylethylidene)-cyclohexanone (X), 5, 5-dimethyl-2-(1-hydroxymethylethyl)-cyclohexanone (IX), 3-hydroxy-5-hydroxymethyl-5-methyl-2-(1-methylethylidene)-cyclo hexano ne (XI), 5,6-dihydro-3,6,6-trimethyl-2(4H)-benzofuranone (VIII), and 5,5-dimethyl-3-hydroxy-2-(1-carboxy ethylidene)-cyclohexanone (XIII). Incubation of compound (I) with phenobarbital (PB)-induced rat liver microsomes in the presence of NADPH resulted in the formation of a metabolite, tentatively identified as a furanoterpene (III) based on proton magnetic resonance, gas chromatography, and gas chromatography-mass spectroscopy analyses. The formation of III was inhibited to a significant extent by carbon monoxide, metyrapone, SKF 525-A, and cytochrome c, suggesting the participation of PB-induced microsomal cytochrome P-450 system in the conversion of I to III. Compound I gave type I spectral change in the PB-induced liver microsomes and the dissociation constant (Ks) for I was 38.5 microM. Intraperitoneal administration of a single dose (250 mg/kg) of I to rats resulted in 26, 23, and 41% decreases in the levels of cytochrome P-450, glucose-6-phosphatase, and aminopyrine N-demethylase, respectively, at the end of 24 h. During this period, a 11-fold increase in serum glutamate pyruvate transaminase level was also observed. However, a decrease in the level of cytochrome P-450 and glucose-6-phosphatase, and an increase in serum glutamate pyruvate transaminase values were comparatively more pronounced when R-(+)-pulegone (250 mg/kg) or CCl(4) (0.6 ml/kg) was administered to rats. Pretreatment of rats with PB potentiated the hepatotoxicity caused by I, whereas pretreatment with 3-methylcholanthrene protected from it. This suggests that PB-induced cytochrome P-450-catalyzed reactive metabolites may be responsible for the toxic effects caused by I. (+info)
Studies on the formation of lipid peroxides and on some enzymic activities in the liver of vitamin E-deficient rats. (2/55)Rats were fed a 5 or 20% casein diet that causes liver necrosis unless supplemented with vitamin E or selenite. The following activities were studied in liver subcellar fractions: enzymic formation of lipid peroxides, NADPH-cytochrome c reductase, oxidative demethylation of aminopyrine, and incorporation of [14C]leucine into protein (with microsomes); xanthine oxidase (with soluble supernatant); and RNA polymerases I and II (with nuclei). Formation of lipid peroxides was higher in rats fed diets without vitamin E and was not reduced significantly by dietary selenite. The activity of xanthine oxidase was higher in animals fed the 20% casein than in those fed the 5% casein diet; however, a higher activity was observed in the rats fed the latter diet without vitamin E or selenite than in those receiving these supplements. The activity of RNA polymerase I was higher in rats fed the low casein diet. Other activities examined were not affected significantly by the level of dietary casein or by vitamin E or selenits. (+info)
Effect of repeated exposure to aniline, nitrobenzene, and benzene on liver microsomal metabolism in the rat. (3/55)Exposure of rats to aniline at daily doses of 50 mg/kg of body weight over a month stimulated the microsomal metabolism as manifested by (1) acceleration of p-hydroxylation of anilin and N-demethylation of aminopyrine in 9-000 times g postmitochondrial supernatant of the liver, (2) shortening the sleeping time after hexobarbital, and (3) reduction of the antipyretic effect of phenacetin. In the rats exposed to nitrobenzene in a similar manner to aniline, nitroreduction of nitrobenzene and p-hydroxylation of aniline remained unaffected; the antipyretic effect of phenacetin was decreased, whereas hexobarbital sleeping time remained unchanged. Exposure of rats to benzene (50 mg/kg of body weight daily for a month) had no effect on the rate of hydroxylation of benzene and N-demethylation of aminopyrine. In benzene-exposed rats hexobarbital sleeping time was prolonged whereas the antipyretic effect of phenacetin was unaffected. Microsomal metabolism of aniline, nitrobenzene, and benzene was stimulated and inhibited when the rats were pretreated with phenobarbital and SKF 525-A, respectively. (+info)
Induction of drug metabolism-related enzymes by methylcholanthrene and phenobarbital in transgenic mice carrying human prototype c-Ha-ras gene and their wild type littermates. (4/55)Transgenic mice hemizygously carrying human c-Ha-ras proto-oncogene, Tg-rasH2 show very sensitive and facilitated carcinogenicity to various carcinogens. In this study, activities of certain enzymes related to drug metabolism and energy metabolism were measured in microsome and cytosol fractions of livers of Tg-rasH2 mice and their wild type littermates with both sexes treated with 3-methylcholanthrene (MC) and phenobarbital (PB). Aminopyrine N-demethylase activities increased significantly in livers of all mice treated with PB. MC and PB treatments induced significant increases in activities of UDP-glucuronosyltransferase and S-adenosyl homocysteinase compared to those in the non-treated groups in microsome fractions from all mice. In cytosol fractions of livers of all mice, glutathione S-transferase activity was significantly induced in the PB treated groups. There were no significant differences in activities of lactate dehydrogenase, glucose 6-phosphate dehydrogenase, pyruvate kinase and glucose 6-phosphatase related to energy metabolism in livers and kidneys among all mice. Tg-rasH2 mice showed stable activities of enzymes related to drug detoxication and energy metabolism similar to those of non-transgenic mice. These results suggest that the human c-Ha-ras transgene may not affect drug metabolism-related enzymes, and the facilitated carcinogenic response in the Tg-rasH2 mouse is not due to these enzymatic disorders. (+info)
Further experiments on lipid peroxidation in transplanted and experimental hepatomas. (5/55)The results of experiments on the subject of lipid peroxidation in hepatomas are described. It is now clear that lipid peroxidation is strongly decreased in most highly dedifferentiated hepatomas. It seems evident that the extent of the decline is strictly related to the degree of dedifferentiation. The model of diethylnitrosamine carcinogenesis, according to the method by Solt, Medline and Farber, has been now adopted to study the stages of carcinogenesis. It was shown that a net decline in lipid peroxidation occurs as early as at the stage of reversible nodules and progresses until the development of clear hepatomas. This change is practically simultaneous with a decline in the efficiency of the enzymes of the drug metabolizing system and in the content of cytochrome P450-Glutathione content and metabolism show also important changes. In fact, a dramatic increase in gamma-glutamyl-transpeptidase takes place very early during carcinogenesis, and is responsible for large decline in total glutathione during incubation of the homogenates. Glutathione peroxidase activity, on the contrary, is decreased, whereas glutathione reductase does not show significant changes. The supernatant of highly anaplastic tumors inhibits lipid peroxidation in normal liver homogenates, suggesting the presence of substances provided with antioxidant properties. These cannot be, however, related to a higher glutathione content. Supernatants from early nodules seem to be unable to block lipid peroxidation in normal liver homogenates. Preliminary experiments done to study the aldehyde pattern produced during lipid peroxidation, both in hepatomas and in nodules, confirm the presence of very poor lipid peroxidation and possibly of different peroxidation kinetics. (+info)
Studies on the evaluation of the toxicity of various salts of lead, manganese, platinum, and palladium. (6/55)Preliminary studies have been conducted on various parameters in order to assess the possible and relative toxicities of a number of metallic salts. Upon oral administration in lethal-dose experiments, two soluble Pt4+ salts were more toxic than the other salts tested. Following intraperiotneal injection in lethal-dose experiments, PbCl2 was less toxic than several of the soluble or partially soluble salts of Pt4+, Pd2+, and Mn2+. An intake of a total of approximately 250 mg of Pt4+ per rat in the drinking fluid over a 30-day interval did not affect the activities of aniline hydroxylase and aminopyrine demethylase in rat liver microsomes. In rats receiving soluble Pt4+ salts in the drinking fluid, the highest concentration of Pt was found in the kidney and an appreciiable concentration was found in the liver. (+info)
Inhibition of human hepatic cytochrome P450s and steroidogenic CYP17 by nonylphenol. (7/55)Effect of nonylphenol on aminopyrine N-demethylase activity, a typical drug-metabolizing enzyme activity, by ten kinds of human hepatic cytochrome P450s (CYP) and on progesterone 17alpha-hydroxylase activity by steroidogenic CYP17 was investigated. When determined at 2 mM substrate concentration, nonylphenol (1 mM) most efficiently inhibited aminopyrine N-demethylation by CYP2C9 and CYP2C19, by 61% and 59%, respectively, followed by CYP2D6, CYP1A2, CYP2C18 and CYP2C8 (46-51%), whereas inhibition of the activities by other CYPs was less than 27%. Additionally, nonylphenol competitively inhibited diclofenac 4'-hydroxylation by CYP2C9 and S-mephenytoin 4'-hydroxylation by CYP2C19 with Ki values of 5.3 and 37 microM, respectively. Furthermore, nonylphenol exhibited a competitive inhibition of progesterone 17alpha-hydroxylase activity by CYP17 with Ki value of 62 microM. These results suggest that nonylphenol inhibits human hepatic CYPs, especially CYP2C9 and CYP2C19, and steroidogenic CYP17 activities. (+info)
Hepatic microsomal enzyme induction in rats fed varietal cauliflower leaves. (8/55)Leaves from a standard, insect-susceptible cauliflower variety and an insect-resistant strain were formulated at either 10 or 25% into semipurified diets for male and female weanling rats. After 3 weeks, relative liver weights, microsomal protein, cytochrome P-450, and activities of hepatic microsomal aminopyrine N-demethylase, aniline hydroxylase, p-nitroanisole O-demethylase, and N-methylaniline N-demethylase were determined. Growth, feed intake, and feed efficiency of male rats were not affected by the inclusion of the dried cauliflower leaf in the diet. However, female rats exhibited a depressed feed intake and increased feed efficiency with cauliflower leaf supplemental diets. Relative liver weights increased with increasing percentage of cauliflower leaves in the diet. Hepatic microsomal enzyme response to cauliflower leaf supplementation of the diet was greater in males than in females. Only aniline hydroxylase activity remained unchanged by the test diets. Male rats showed significant increases in N- and O-demethylation with both the 10 and 25% cauliflower diets, and increased values for microsomal protein and cytochrome P-450 at the 25% supplemental level. Female rats did not show significant hepatic microsomal induction from cauliflower leaf consumption at the 10% level. However, cytochrome P-450 and the metabolism of aminopyrine and p-nitroanisole were enhanced by consumption of cauliflower leaves at 25% of their diet. None of the parameters tested in this study evidenced a difference between the two cauliflower cultivars fed to either sex. (+info)
The development of several hepatic microsomal drug-metabolizing enzyme activities in rats was studied in relation to its androgen dependence during the neonatal period. Rats with their androgen deprived during the neonatal period (female rats and male rats castrated at birth) respond less to androgen treatment at adulthood for the metabolism of aminopyrine, ethylmorphine and hexobarbital as compared to rats exposed to neonatal androgen (male rats, male rats castrated at birth but neonatally treated with androgen and male rats castrated at the age of 20 days). This difference in the degree of hepatic responsiveness, however, varied with the substrates: ethylmorphine N-demethylase activity was affected the most whereas aminopyrine N-demethylase and hexobarbital oxidase activities were only marginally affected. Furthermore, these differences in responsiveness seem to be a delayed event, since hepatic aminopyrine N-demethylase activity in rats castrated at birth did respond to androgen stimulation ...
Alteration of drug metabolizing enzymes in sulphite oxidase deficiency | AVESİS
The aim of this study was to investigate the possible effects of sulphite oxidase (SOX, E.C. 184.108.40.206) deficiency on xenobiotic metabolism. For this purpose, SOX deficiency was produced in rats by the administration of a low molybdenum diet with concurrent addition of 200 ppm tungsten to their drinking water. First, hepatic SOX activity in deficient groups was measured to confirm SOX deficiency. Then, aminopyrine N-demethylase, aniline 4-hydroxylase, aromatase, caffeine N-demethylase, cytochrome b5 reductase, erythromycin N-demethylase, ethoxyresorufin O-deethylase, glutathione S-transferase, N-nitrosodimethylamine N-demethylase and penthoxyresorufin O-deethylase activities were determined to follow changes in the activity of drug metabolizing enzymes in SOX-deficient rats. Our results clearly demonstrated that SOX deficiency significantly elevated A4H, caffeine N-demethylase, erythromycin N-demethylase and N-nitrosodimethylamine N-demethylase activities while decreasing ethoxyresorufin ...
We have previously described the development of genetic models to study the in vivo functions of the hepatic cytochrome P450 system, through the hepatic deletion of either cytochrome P450 oxidoreductase (POR; HRN line) or cytochrome b5 (Cyb5; HBN line). However, HRN mice still exhibit low levels of mono-oxygenase activity, in spite of the absence of detectable reductase protein. To investigate whether this is because cytochrome b5 and cytochrome b5 reductase can act as sole electron donors to the P450 system, we have crossed HRN with HBN mice to generate a line lacking hepatic expression of both electron donors (HBRN). HBRN mice exhibited exacerbation of the phenotypic characteristics of the HRN line - liver enlargement, hepatosteatosis and increased expression of certain cytochrome P450s. Also, drug metabolising activities in vitro were further reduced relative to the HRN model, in some cases to undetectable levels. Pharmacokinetic studies in vivo demonstrated that midazolam half-life, Cmax and ...
Biochemical, histopathological and ultrastructural changes in rat liver induced by r-( + )-pulegone, a monoterpene ketone -...
Biochemical, histopathological and ultrastructural changes occurring at different time points after intraperitoneal administration of a single dose of pulegone (300 mg/kg) were studied. Significant decreases in the level of liver microsomal cytochrome P-450 (67%), heme (37%), aminopyrine N-demethylase (60%) and glucose-6-phosphatase (58%), were noticed 24 hr after pulegone treatment. Alanine amino transferase (ALT) levels increased in a time dependent manner, following exposure of rats to pulegone. Light microscopic studies of liver tissues showed dilation of central veins and distention of sinusoidal spaces 6 hr after pulegone treatment. Initial centrilobular necrosis was noticed at 12 hr. Centrilobular necrosis became severe at 18 hr and nuclear changes included karyorrhexis and karyolysis. Midzonal and periportal degenerative changes in addition to centrilobular necrosis was observed 24 hr after pulegone administration. Electron microscopic changes showed severe degeneration of endoplasmic ...
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2 major biochemical reactions that occur in the peroxisome is the mixed function oxidase and the other is a catalase but what 2 enzymes are involved in these reactions?...beta oxidation one of them ...
The effect of a 20-day administration of aminopyrine (600 mg kg-1) as well as two metabolites of aminopyrine, 4-aminoantipyrine (525 mg kg-1) and 4-acetamidoantipyrine (635 mg kg-1), on several hepatic, kidney and serum enzyme activities were investigated. In the aminopyrine-treated group, a pronounced induction of gamma-glutamyl transpeptidase was shown in the whole homogenate as compared to that in the hepatic microsomes. Serum gamma-glutamyl transpeptidase activity was also increased by administration of aminopyrine or 4-aminoantipyrine. No change in gamma-glutamyl transpeptidase activity was observed in kidney and hepatic cytosolic fractions. In all cases, 4-acetamidoantipyrine treatment showed no significant change in the enzyme activities tested. Under the same experimental condition, the amounts of cytochrome P-450 and b5, the activities of aminopyrine N-demethylase, aniline hydroxylase and carboxylesterase of liver microsomes were all induced in the aminopyrine- and ...
Clotrimazole, an N-substituted imidazole widely used as an antifungal agent, has been shown to both inhibit and induce hepatic cytochrome P-450 and related monooxygenase activities. In this study the profile of hepatic cytochrome P-450 isozyme(s) induced by clotrimazole treatment of male Sprague-Dawley rats was investigated. Clotrimazole administration (100 mg/kg, daily for 4 days, ig) resulted in 86% induction of spectrally detectable cytochrome P-450 in hepatic microsomes. In these microsomes 7-ethoxycoumarin O-deethylase (126%), aminopyrine N-demethylase (176%), benzphetamine N-demethylase (117%), p-nitrophenol hydroxylase (89%), and 7-ethoxyresorufin O-deethylase (62%) activities were significantly induced, whereas aryl hydrocarbon hydroxylase activity remained unchanged. Characterization of cytochrome P-450 isozyme(s) in hepatic microsomes prepared from clotrimazole-treated animals was based on the immunoreactivity of these microsomes with highly specific monoclonal antibodies (MAbs) raised ...
Volume 45, Number 2 : HUSCAP
MARUMO, Sativa (1997) Regio- and stereoselective propranolol metabolism by 3 forms of purified cytochrome P450 from rat liver and the effect of cytochrome b5 on these metabolisms. Japanese Journal of Veterinary Research, 45(2): 135-136. MAEDA, Yutaka (1997) Strain differences in age-associated change in Testosterone 6β-hydroxylation in Wistar and Dark Agouti rats. Japanese Journal of Veterinary Research, 45(2): 135-135. NIKAIDOU, Satoshi (1997) Effect of green tea on hepatic enzyme activities and mutagenic transformation of benzo[a]pyrene. Japanese Journal of Veterinary Research, 45(2): 134-134. OHKURA, Kaori (1997) Three-dimensional visualization of abdominal and thoracic organs of rodents by superimposing MRI multislices with a computer-graphic technique. Japanese Journal of Veterinary Research, 45(2): 133-133. UI, Masahiro (1997) Transcriptional analysis of Mareks disease virus (MDV) genes in MDV-transformed lymphoblastoid cells without activated cells. Japanese Journal of Veterinary ...
Ligand binding of safrole to cytochrome P-450. - Surrey Research Insight Open Access
Safrole, a hepatocarcinogen, is converted by the microsomal mono-oxygenase system to a reactive intermediate which interacts with cytochrome P-450 to form a ligand complex. The formation of this complex is accompanied by loss of mono-oxygenase activity. The present study describes the interaction of the safrole reactive intermediate with microsomes from phenobarbital, 3-methylcholanthrene and safrole pretreated animals.. ...
Ethanol-inducible cytochrome P450 (P450IIE) is reported to be induced by ketosis. In the present study, the effects of a high fat diet on P450IIE induction and the relationship between ketone body concentration and P450IIE induction were studied by the following: 1) measurement of the activity of aniline hydroxylase, 2) immunoblot analysis for P450IIE protein, and 3) Northern blot analysis for P450IIE mRNA. The enzyme activities (aniline hydroxylase) in hepatic and renal microsomes were elevated about 2-3-fold by feeding with a high fat diet for 3 days. The increases in enzyme activities were also accompanied by 3-fold increases in immunoreactive P450IIE protein and its mRNA. In contrast, no differences were observed for the catalytic activities of N-alkoxyresorufin dealkylases or the amounts of immunoreactive P450IA and P450IIC, indicating a specific induction of P450IIE by high fat feeding. Furthermore, the increases in the levels of P450IIE mRNA correlated positively (r = 0.73) with plasma ...
VALUE OF AMINOPYRINE*† | Annals of Internal Medicine | American College of Physicians
Modern medical science has made such tremendous strides in the field of therapeutics that many older drugs, especially if potentially productive of untoward and dangerous side-effects, have been discredited and have fallen into disuse. Admittedly, many such drugs have marked therapeutic value and are deserving of further investigation and clinical trial before they are completely discarded.. Aminopyrine, by force of historical circumstances, has been such a discredited drug for many years.1, 2a, 3c, 4b, 5, 6, 7, 8 The danger of agranulocytosis resulting from its use is real and well established, but undoubtedly has been overemphasized.1, 2a, 4b, 5, 6, ...
Amidopyrine synonyms, amidopyrine antonyms - FreeThesaurus.com
Synonyms for amidopyrine in Free Thesaurus. Antonyms for amidopyrine. 1 synonym for amidopyrine: aminopyrine. What are synonyms for amidopyrine?
Inhibition of dexamethasone-induced cytochrome P450-mediated mutagenicity and metabolism of aflatoxin B1 by Chinese medicinal...
Oldenlandia diffusa (OD) and Scutellaria barbata (SB) have been used in traditional Chinese medicine for treating liver, lung and rectal tumours. We previously showed that they inhibited mutagenesis, DNA binding and metabolism of aflatoxin B1 (AFB1) and benzo(a)pyrene (BaP) bioactivated by Aroclor 1254-induced rat S9. The purpose of this study was to investigate the effects of OD and SB on the mutagenicity of AFB1 in Salmonella typhimurium TA100 using dexamethasone (DXM)-induced rat hepatic S9, on cytochrome P450-linked aminopyrine N-demethylase (APND) activity in DXM-induced hepatic microsomes and on the metabolism of AFB1 by DXM-induced S9 using high-performance liquid chromatography (HPLC). The experimental results showed that OD and SB consistently inhibited the mutagenicity of AFB1 bioactivated by either non-induced or DXM-induced S9. These effects correlated with the inhibition of cytochrome P450-linked APND activity in DXM-induced microsomes and with an inhibition of DXM-induced S9 mediated
Effect of α-tocopherol on hepatic mixed function oxidases in hepatic ischemia/reperfusion - Lee - 2005 - Hepatology - Wiley...
Lee, S.-M. and Clemens, M. G. (1992), Effect of α-tocopherol on hepatic mixed function oxidases in hepatic ischemia/reperfusion. Hepatology, 15: 276-281. doi: 10.1002/hep.1840150217 ...
SID.ir | EFFECTS OF 3 - METHYL CHOLANTHEREN ON LIVER MICROSOMAL MIXED FUNCTION OXIDASE ACTIVITIES OF ACIPENSER PERSICUS
Download Free Full-Text of an article EFFECTS OF 3 - METHYL CHOLANTHEREN ON LIVER MICROSOMAL MIXED FUNCTION OXIDASE ACTIVITIES OF ACIPENSER PERSICUS
The N-demethylation of N,N-dimethylphenobarbital by isolated rat hepatocytes was increased severalfold by pretreatment of animals with sodium phenobarbital (75 mg/kg/day ip for 3 days). The apparent extent of induction depended on the length of the cell incubation, being 3-fold when calculated after a 5-min incubation and 5-fold when calculated after a 60-min incubation. This difference was due to the fact that metabolism by induced hepatocytes more closely approached linearity over a 60-min period. Microsomal cytochrome P-450 levels were increased 3-fold upon phenobarbital treatment; during incubations of less than 30 min duration, increased N-demethylase activity could be entirely accounted for by this increase in cytochrome P-450. When protein levels were measured directly in microsomes isolated from hepatocyte homogenates, the levels in induced hepatocytes were approximately 45% higher than control. However, phenobarbital treatment had no significant effects on total cellular protein or ...
DILANTIN® Infatabs® Drug Interactions (phenytoin) | Pfizer Medical Information - Australia
Phenytoin is extensively bound to plasma proteins and is prone to competitive displacement. Phenytoin is metabolized by hepatic cytochrome P450 enzymes CYP2C9 and CYP2C19, and is particularly susceptible to inhibitory drug interactions because it is subject to saturable metabolism. Inhibition of metabolism may produce significant increases in circulating phenytoin concentrations and enhance the risk of drug toxicity. Monitoring of phenytoin serum levels is recommended when a drug interaction is suspected.. Phenytoin is a potent inducer of hepatic drug-metabolizing enzymes. ...
DGIdb - TRIMETHADIONE Drug Record
DGIdb, The Drug Gene Interaction Database, is a research resource that can be used to search candidate genes or drugs against the known and potentially druggable genome.
BR-4935 Summary Report | CureHunter
BR-4935: cardiotonic; a non-adenosine analog adenosine1-receptor agonist with a substituted 2-thio-3,5-dicyano-4-phenyl-6-aminopyrine
Variations of some parameters of enzyme induction in chemical workers. | IRIS Università degli Studi di Firenze
Abstract Several parameters related to mono-oxygenase activity were followed in a population of chemical workers and controls. Workers exposed to toluene and xylene had a significant increase of urinary glucaric acid, that was correlated with hippuric acid excretion. On the other hand, workers exposed to pigments showed a marked increase of antipyrine half-life. A dose-related decrease of liver N-demethylase was induced in rats by the administration of a mixture of three of the pigments in use in the plant. Serum gamma-glutamyltranspeptidase was decreased in the workers exposed to pigments, but this variation was not statistically significant. The exposure to different chemicals in the workplace seemed to induce a complicated variation of mono-oxygenase levels, some enzyme being inhibited and others induced in the same group of workers. The sensitivity of these workers to toxic effects of chemicals, carcinogenic compounds and drugs seems to differ markedly from the control population. © 1982 ...
Tridione (Trimethadione Tablets): Side Effects, Interactions, Warning, Dosage & Uses
Learn about Tridione (Trimethadione Tablets) may treat, uses, dosage, side effects, drug interactions, warnings, patient labeling, reviews, and related medications.
Most recent papers with the keyword Multidrug resistant organism | Read by QxMD
ETHNOPHARMACOLOGICAL RELEVANCE: Shikonin, a naphthoquinone pigment abundant in the root of the Chinese herb Lithospermum erythrorhizon, has been widely used to treat inflammatory diseases for thousands of years. Whether shikonin changes drug metabolism remains unclear. AIM OF THE STUDY: We investigated whether shikonin modulates the expression of hepatic drug-metabolizing enzymes and transporters as well as the possible mechanisms of this action. MATERIALS AND METHODS: Primary hepatocytes isolated from Sprague-Dawley rats were treated with 0-2 μM shikonin and the protein and mRNA levels of drug-metabolizing enzymes and transporters as well as the activation of aryl hydrocarbon receptor (AhR) and NF-E2-related factor 2 (Nrf2) were determined ...
Steroid modulation of liver regeneration and hepatic microsomal enzymes in rats of either sex.
Rats of either sex as intact or partially hepatectomized (two-thirds liver removal) were injected s.c. daily for 7 days post-operatively with natural and synthetic estrogens, androgens or anabolic steroids, progesterone and adrenal cortical hormones
Aminopyrine Aminophenazone AmidopyrineCAS:58-15-1 - Wuhan Carphetin Chemical Co.,LTD - ecplaza.net
MF:C13H17N3O MW:231.29 CAS:58-15-1 EINECS:200-365-8 Apperance:White fronds crystal or crystalline powder. No smell, taste slightly bitter. Product use: Antipyretic and analgesic, used for fever and headache, joint pain, neuralgia, dysmenorrhea and...
Bardag-Gorce, F., Yuan, Q.X., Li, J., French, B.A., Fang, C., Ingelman-Sundberg, M., French, S.W., 2000. The effect of ethanol-induced cytochrome p4502E1 on the inhibition of proteasome activity by alcohol. Biochem. Biophys. Res. Commun. 279, 23-29.. Bondoc, F.Y., Bao, Z., Hu, W.Y., Gonzalez, F.J., Wang, Y., Yang, C.S., Hong, J.Y., 1999. Acetone catabolism by cytochrome P450 2E1: studies with CYP2E1-null mice. Biochem. Pharmacol. 58, 461-463.. Cederbaum, A.I., 2014. Methodology to assay CYP2E1 mixed function oxidase catalytic activity and its induction. Redox Biol. 2C, 1048-1054.. Cheng, J., Chen, C., Kristopher, K.W., Manna, S.K., Scerba, M., Friedman, F.K., Luecke, H., Idle, J.R., Gonzalez, F.J., 2013. Identification of 2-piperidone as a biomarker of CYP2E1 activity through metabolomic phenotyping. Toxicol. Sci. 135, 37-47.. Cheung, C., Gonzalez, F.J., 2008. Humanized mouse lines and their application for prediction of human drug metabolism and toxicological risk assessment. J. Pharmacol. Exp. ...
2,8-diiododibenzo[b,d]furan | CYP450-cyp3a-cyp450.com
Product Name: 2,8-diiododibenzofuranFormula: C12H6OI2Weight: 419.98344SMILES: IC1=CC2C3=C(C=CC(I)=C3)OC=2C=C1CAS NO: 127-48-0 Product: Trimethadione &
Lentinan: Uses, Side Effects, Interactions, Dosage, and Warning
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Functional analysis of chromosome 18 in pancreatic cancer: strong evidence for new tumour suppressor genes.
Autori: Lefter LP, Sunamura M, Furukawa T, Yastsuoka T, Abe H, Inoue H, Abe T, Egawa S, Miura K, Morita R, Horii A, Matsuno S.. Editorial: Asian J Surg. 2004 Apr;27(2):85-92., 2004.. Rezumat:. BACKGROUND: In a previous work, we demonstrated that loss of heterozygosity of 18q is a frequent event significantly associated with poor prognosis in pancreatic cancer. We hypothesized that restoration of heterozygosity of chromosome 18 in pancreatic cancer cells would reduce their tumorigenicity. This study was intended to provide functional evidence for the existence of new tumour suppressor gene(s) located on chromosome 18. METHOD: Restoration of heterozygosity was achieved by introducing a normal copy of chromosome 18 into pancreatic ductal carcinoma using a microcell-mediated chromosome transfer technique. The tumorigenicity and metastatic ability of both the parental cells and resulting hybrids were assessed in vitro and in vivo. RESULTS: In vitro growth of hybrid clones was significantly delayed ...
Hepatic Microsomal Induction in Rat Liver: Heterogeneity of Response<...
TY - JOUR. T1 - Hepatic Microsomal Induction in Rat Liver. T2 - Heterogeneity of Response. AU - Kiernan, Thomas W. AU - LUISADA‐OPPER, A.. AU - ERTEL, NORMAN H.. PY - 1981/1/1. Y1 - 1981/1/1. N2 - Abstract: The concept that urinary 6‐hydroxycortisol excretion patterns reflect all microsomal enzyme activities remains a subject of controversy. Three different microsomal hydroxylase activities were therefore studied in rat liver after the animals were intoxicated with alcohol, phenobarbital, or a combination thereof. The activities of pentobarbital hydroxylase, aniline hydroxylase, and cortisol 6‐hydroxylase (the enzyme metabolizing cortisol to 6‐hydroxycortisol) were assayed simultaneously after each treatment. A heterogeneity of enzyme activities was noted. Cortisol 6‐hydroxylase activity did not parallel the activity patterns of the two other hydroxylases. The data suggest that before urinary 6‐hydroxycortisol excretion patterns can be utilized as an index of microsomal enzyme ...
Regulatory Role of Arginine-Specific Mono(ADP-Ribosyl)Transferase in Muscle Cells | Springer for Research & Development
Earlier we demonstrated that meta-iodobenzylguanidine (MIBG), a specific inhibitor of arginine mono-ADP-ribosylation blocks proliferation and differentiation of chick skeletal myogenic cells in...
Epigenase JMJD3/UTX Demethylase Activity/Inhibition Assay Kit (Fluorometric) | EpiGentek
The Epigenase JMJD3/UTX Demethylase Activity/Inhibition Assay Kit (Fluorometric) is a complete set of optimized reagents, designed for measuring activity/inhibition of JMJD3 and UTX using nuclear extracts or purified enzymes...
Dose dependence of the14C-aminopyrine breath test | SpringerLink
Although the aminopyrine breath test has received much attention, the question has not yet been settled whether pharmacological or tracer doses of the drug should be used. Nine volunteers were given14...
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Molecular and functional characterization of drug-metabolizing enzymes and transporter expression in the novel spontaneously...
In toxicological research, immortalized human hepatocytes provide a useful alternative to primary hepatocytes because interindividual variability in the expression of drug-metabolizing enzymes and drug transporters can largely be eliminated. However, it is essential that the cell line retain the original phenotype. The purpose of this study was to characterize a novel spontaneously immortalized human hepatocyte cell line, HC-04, with respect to the transcript and functional protein expression profile for the major drug-metabolizing enzymes and transmembrane transporters. HC-04 cells retained hepatocyte-specific function including albumin production and ornithine transcarbamoylase and glucose-6-phosphatase activity. Most of the major CYP forms were expressed at basal levels and responsive to inducing agents. In particular, CYP3A4 was expressed abundantly, and HC-04 cells were able to metabolize the CYP3A4 probe, midazolam, at a rate similar to primary human hepatocytes. Furthermore, the major ...
DNA Demethylase Activity/Inhibition Assay Kit - (KA0677) - Products - Abnova
DNA Demethylase Activity/Inhibition Assay Kit is use for measuring DNA demethylase activity/inhibition. (KA0677) - Products - Abnova
The effect of ethanol on drug oxidations in vitro and the significance of ethanol-cytochrome P-450 interaction | Biochemical...
The effect of ethanol on N-demethylation of aminopyrine in rat liver slices and in the microsomal fraction and on microsomal hydroxylation of pentobarbital and aniline was studied. With liver slices N-demethylation of aminopyrine was stimulated by 35-40% at low ethanol concentrations (2mm), whereas no stimulation occurred at high concentrations (100mm). With the liver microsomal fraction, an inhibitory effect was observed only at high ethanol concentrations (100mm). This was also observed with the other drugs studied. In agreement with these results, only at a high concentration did ethanol interfere with the binding of drug substrates to cytochrome P-450. Further, as previously reported, ethanol produced a reverse type I spectral change when added to the liver microsomal fraction. Evidence that this spectral change is due to removal of substrate, endogenously bound to cytochrome P-450, is reported. A dual effect of ethanol is assumed to explain the present findings; in liver slices, at a low ...
INFLUENCE OF BACKGROUND GENOME ON ENZYMATIC CHARACTERISTICS OF YELLOW (Ay/-, Avy/-) MICE | Genetics
dentification of the fundamental polypeptide difference between yellow (Ay/-, Avy/-) and non-yellow mice is important for biomedical research because of the influence of the yellow genotype on normal and neoplastic growth and obesity. The complexity of the yellow mouse syndrome makes attainment of this objective dependent on the separation of those pleiotropic enzyme differences which are secondary, and depend on the background genome, from those which are primary, and depend primarily on the agouti locus genotype.-Four of nine hepatic enzyme activities assayed simultaneously differed between eight-week-old yellow (Ay/-, Avy/-) and non-yellow (A/-, a/a) male inbred and F1 hybrid mice. Among these four, only cytoplasmic malic enzyme activity was elevated in all yellow mice, as compared with the non-yellow sibs, regardless of background genome. Glucokinase, serine dehydratase, and tyrosine α-ketoglutarate transaminase activities were also changed in yellow mice, but these alterations depended ...
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Formic Acid Rubber Pulp Disinfest tianjin - WorldBid B2B Market
Chemicals - Coatings China, Formic Acid Rubber Pulp Disinfest, Uses: 1. Pharmaceutical industry: Caffeine, Enzimes, Aminopyrine, Vitamin B1 2. Pesticide industry: Triazolone, Disinfes...
Induction of N-nitrosodimethylamine metabolism in liver and lung by in vivo pyridine treatments of rabbits | AVESİS
N-Nitrosodimethylamine is a procarcinogen that is activated by cytochrome P450 dependent N-nitrosodimethylamine N-demethylase to labile alpha-carbon hydroxylated products further resulting in active methylating agents. In vivo intraperitoneal administration of pyridine to rabbits significantly increased N-nitrosodimethylamine N-demethylase activity by 6.9- and 5.2-fold in liver and lung microsomes, respectively. Although, p-nitrophenol hydroxylase and aniline 4-hydroxylase activities were markedly enhanced by pyridine treatment in liver about 4.4- and 5.8-fold, respectively, no change was observed in the activities of these enzymes in lung microsomes. Pyridine treatment also elevated P450 contents of liver and lung by 2.04- and 1.4-fold, respectively. SDS-PAGE of pyridine-induced liver microsomes revealed a protein band of enhanced intensity having M-r of 51,000 migrating in the region of cytochrome P4502E1. The results obtained in this study demonstrated for the first time, a significant ...
Trimethadione anticonvulsant drug - Stock Image F011/3640 - Science Photo Library
Trimethadione anticonvulsant drug molecule. Used in treatment of seizures. Atoms are represented as spheres with conventional colour coding: hydrogen (white), carbon (black), oxygen (red), nitrogen (blue). - Stock Image F011/3640
The kinetics of ethylmorphine N-demethylation in isolated hepatocytes: The effect of drug transport and oxygen concentration<...
TY - JOUR. T1 - The kinetics of ethylmorphine N-demethylation in isolated hepatocytes. T2 - The effect of drug transport and oxygen concentration. AU - Erickson, R. R.. AU - Yu-Drent, P.. AU - Holtzman, J. L.. PY - 1982/1/1. Y1 - 1982/1/1. N2 - In previous studies we have observed that the K(m) for ethylmorphine N-demethylase was only 50 to 100 μM in the isolated hepatocyte as opposed to 250 to 340 μM in isolated microsomes. These data suggested that either the drug is actively transported into the cell or that the enzymes in the cell had a reduced turnover number. Our current study indicates that ethylmorphine is transported into the hepatocyte with a K(m) of 250 μM and Tm of 20 nmol/min 106 cells. On the basis of temperature and inhibitor studies, this would appear to be active transport. When the turnover number of the ethylmorphine N-demethylase was decreased by reducing the oxygen from 20% of the gas phase to 5%, the activity was partially uncompetitively inhibited with the K(m) ...
The Histone Demethylase Activity of Rph1 is Not Essential for Its Role in the Transcriptional Response to Nutrient Signaling
Rph1 and Gis1 are two related yeast zinc finger proteins that function as downstream effectors in the Ras/PKA, TOR and Sch9 nutrient signaling pathways. Both proteins also contain JmjC histone demethylase domains, but only Rph1 is known to be an active enzyme, demethylating lysine 36 of histone H3. We have studied to what extent the demethylase activity of Rph1 contributes to its role in nutrient signaling by performing gene expression microarray experiments on a yeast strain containing a catalytically inactive allele of RPH1. We find that the enzymatic activity of Rph1 is not essential for its role in growth phase dependent gene regulation. However, the ability of Rph1 to both activate and repress transcription is partially impaired in the active site mutant, indicating that the demethylase activity may enhance its function in vivo. Consistent with this, we find that the Rph1 mutation and a deletion of the histone H3 methylase Set2 affect the same target genes in opposite directions. Genes that ...
In Vitro Technology - International Society for the Study of Xenobiotics
With that encouragement Axelrod quickly showed that amphetamine was rapidly metabolized in rabbit liver slices. He went on to define the co-factor requirements for the reaction finding that NADPH was required; similar to observations of Bert LaDu in his studies on aminopyrine demethylation9.Axelrod then determined the sub-cellular location of the activity by using methods developed by Hogeboom and Schneider10. This newly defined microsomal oxidizing system was soon shown to be responsible for the metabolism of a wide variety of drugs and other chemicals.. Ref: 1V.R.Potter and C.A.Elvehjem, J.Biol. Chem. ,114:495-504 (1936) 2A.Claude, Cold String Harbor Symposium Quant.Biol.,9:263-270 (1941)3 W.C.Schneider,J.Biol.Chem. 165:585-593 (1949) 4E.C.Miller and J.A.Miller, Cancer Res. 7:468-480 (1947)5 E.S.Stevenson, K. Dobriner, and C.P.Rhoads, Cancer Research 2:160-167 (1942)6 G.C.Mueller and J.A.Miller, J.Biol.. Chem. 176:535-544 (1948) 7G.C.Mueller and J.A. Miller, J.Biol. Chem. ,180:1125-1236 ...
Algorithm for the management of hepatic enzyme elevatio | Open-i
Algorithm for the management of hepatic enzyme elevations in the INPULSIS® trials. *Defined as increase in hepatic transaminases (AST or ALT) to ≥3 x ULN, an
Aniline | 62-53-3
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Honda NSX Spiritual Successor In Development | Drive
Hondas NSX hero car is to make its fabled return to the supercar market after all, according to reports out of the Shanghai Auto Show this week.. Earlier reports claimed the new NSX would be downgraded from supercar to six-cylinder sports car, built on a reversed version of the Accord platform.. According to US industry paper Autonews however, Honda CEO Takanobu Ito has confirmed that a spiritual successor to the NSX is in development.. Dont expect a big Lexus LFA-beating V10 engine however: no details have been confirmed, but Honda is understood to have ruled out the big engine approach, going instead for an environmentally-friendly hybrid drivetrain.. Thats the kind of sports car we want to make, Ito reportedly told Autonews at the Shanghai show this week, describing a car that would be exhilirating to drive and environmentally-friendly.. Dropping the planned V10 heart in favour of a hybrid approach suggests Honda is watching closely BMWs Vision EfficientDynamics-based hybrid ...
Mass Spectrometry-Based Quantitative Proteomics for Drug-Metabolizing Enzymes
Quantification of highly homologous human liver drug-metabolizing enzymes (DMEs) has been a challenging task in drug metabolism and disposition research, due to a lack of specific antibodies and marker substrates. Mass spectrometry (MS) techniques and applications have evolved significantly, striving to achieve absolute and specific quantification of these enzymes. Since the first absolute quantification of cytochrome P450s (CYPs) using the attachment of isotope tags to free thiols (iCAT), MS-based quantification has become much more versatile, cheaper, and easier to use. Today, variations of liquid chromatography-multiple reaction monitoring (LC-MRM)-based targeted proteomics, such as AQUA (absolute quantification) and QconCAT (concatenated signature peptides), have become the gold standards for quantification. These new methods have driven the absolute quantification of DMEs to become a routine laboratory task. Many drug metabolism-related projects require absolute enzyme quantification. For ...
adenomas eosinophilic drug 2000:2010[pubdate] *count=100 - BioMedLib™ search engine
Primary treatment-related effects included: initial sedation of animals exposed to 3,000 ppm and its subsequent resolution correlating with induction of hepatic mixed function oxidase activity and S-phase DNA synthesis; elevated mortality in high-exposure male rats and mice (chronic study); elevated deposition of alpha2u-globulin (alpha2U-G) and associated nephropathy and S-phase DNA synthesis in male rat kidneys; accelerated atrophy of the adrenal gland X-zone in female mice (subchronic study only); and increased occurrence and/or severity of eosinophilic foci of altered hepatocytes in male rats ...
Nonylphenol, branched, ethoxylated - Registration Dossier - ECHA
Oral The metabolism of nonyl phenol ethoxylates takes place by shortening the ethylene oxide chain, carboxylation of the alkyl chain by omega oxidation and subsequent glucoronide and sulphate conjugation (CIR, 1983). Knaak et al. (1966) fed 67 mg/kg of NP-14C TP-9 (a commercial product containing on average 9 moles of EO per mole of NP, i.e. NPE-9) or 14C-NP to rats and followed urinary, faecal over a period of 7 days and CO2 excretion over a period of 4 days. Within the observed time period, 20% of the NP-14C TP-9 was excreted in urine, 78% in faeces and none as14CO2 14C-NP per se was found to be excreted in a similar manner. In the same study, Knaak et al. also exposed rats to 7, 10, 12 and 15 mole adducts of NP isolated from ethylene oxide-labelled TP-9. The 12 and 15 mole adducts were excreted to a greater extent in the feces than the 7 and 10 mole adducts, while the reverse situation occurred in urine. Modelling the metabolism of NPE-2 and NPE-4 in liver using the using prediction tools ...
1984 Archives - ToxStrategies
Hasegawa R, St John MK, Cano M, Issenburg P, Klein DA, Walker BA, Jones JW, Schnell RC, Merrick BA, Davies MH, McMillan DA, Cohen SM. 1984. Bladder freeze ulceration and sodium saccharin feeding in the rat, examination for urinary nitrosamines, mutagens, and bacteria, and effects on hepatic microsomal enzymes. Food Chem Toxicol 22:935-942.. View Abstract ...
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Table of Contents - May 01, 1992, 20 (3) | Drug Metabolism & Disposition
Frontiers | Geraniol Pharmacokinetics, Bioavailability and Its Multiple Effects on the Liver Antioxidant and Xenobiotic...
Aminopyrine N-demethylase (APND) activity-CYP3A1/2. Activity was determined by the quantification of CH2O release, according to ... The total incubation volume was 3 ml, composed of 0.5 ml water solution of 50 mM aminopyrine and 25 mM MgCl2, 1.48 ml of 0.60 ... Incubation mixture consisted of 2.6 ml, composed of 1 mM ethoxycoumarin, 5 mM MgCl2, NADPH-generating system (see aminopyrine ... Acetonitrile (PubChem CID:6342), aminopyrine (PubChem CID:6009), bovine serum albumin, dichlorophenolindophenol (PubChem CID: ...
NIOSHTIC-2 Search Results - Basic View
Kevin Fish | Stanford Medicine
The activity rate of aminopyrine N-demethylase was not changed by the diet; however, p-nitroanisole O-demethylase activity was ... The activity rate of aminopyrine N-demethylase was not changed by the diet; however, p-nitroanisole O-demethylase activity was ... The activity rate of aminopyrine N-demethylase was not changed by the diet; however, p-nitroanisole O-demethylase activity was ...
METHYL ISOBUTYL KETONE - Some Chemicals Present in Industrial and Consumer Products, Food and Drinking-Water - NCBI Bookshelf
Induction by kopsinine of hepatic mixed-function oxidase in mice - HUANG - Acta Pharmacologica Sinica
786. Ethyl-1-hexanol, 2- (WHO Food Additives Series 32)
Registration Dossier - ECHA
Aminopyrine N-Demethylase Entry term(s). Aminopyrine N Demethylase Demethylase, Aminopyrine N N Demethylase, Aminopyrine N- ... Aminopyrine N-demethylase Entry term(s):. Aminopyrine N Demethylase. Demethylase, Aminopyrine N. N Demethylase, Aminopyrine. N- ... Aminopyrine N-Demethylase - Preferred Concept UI. M0000966. Preferred term. ...
IMSEAR at SEARO: Inhalation toxicity studies of methyl isocyanate (MIC) in rats: Part III--Studies on biotransformation enzymes.
DeCS 2016 - June 12, 2016 version
Pesquisa | Prevenção e Controle de Câncer
Aminopyrine N-Demethylase [D08.811.682.662.582.276] * Cytochrome P-450 CYP2E1 [D08.811.682.662.582.338] ... AlkB Homolog 5, RNA Demethylase [D08.811.682.662.582.207] * Alpha-Ketoglutarate-Dependent Dioxygenase FTO [D08.811.682.662. ... AlkB Homolog 4, Lysine Demethylase [D08.811.682.662.582.138] * ... Ethylmorphine-N-Demethylase [D08.811.682.662.582.400] * Histone ...
DeCS 2019 - June 12, 2019 version
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No data available that match "aminopyrine n demethylase"
- Caffeine demethylase activity in human and Dark Agouti rat liver microsomes. (aspetjournals.org)
- Metyrapon, a specific inhibitor of cytochrome P-450, partially antagonized aminopyrine demethylase activity of microsomes from mice treated with kopsinine. (chinaphar.com)
- no significant changes were noted in liver aminopyrine - N - demethylase or aniline hydrolase activities. (cdc.gov)
- Oral administration of kopsinine 200 mg/kg once daily for 3 d significantly increased liver microsomal protein, cytochrome P-450, cytochrome b5, NADPH-cytochrome C reductase, aminopyrine demethylase and benzo(a)pyrine hydroxylase activities in mice. (chinaphar.com)
- Comparison with aminopyrine N-demethylase activity. (aspetjournals.org)
- No significant changes in P-450 content or in the following activities were noted: ethylmorphine- N - demethylase, lauric acid 1 1-hydroxylation, and lauric acid 12-hydroxylation. (cdc.gov)
- no significant changes were noted in liver aminopyrine - N - demethylase or aniline hydrolase activities. (cdc.gov)
- The sesquiterpene lactones (STL) helenalin and alantolactone were effective in vitro inhibitors of the mouse hepatic microsomal mixed-function oxidase (MFO) enzymes, aminopyrine demethylase (APD), aniline hydroxylase (ANH) and 7-ethoxyresorufin deethylase (ERD). (nih.gov)
- A preclinical study in rats demonstrated that single doses of garlic oil (500 mg/kg intraperitoneally [i.p.]) resulted in a significant depression of hepatic CYP450, aminopyrine N-demethylase, and aniline hydroxylase activity. (medscape.com)
- Male rats inhaling air containing o-xylene, 4750 mg/cu m/8 hr/day, for 1 yr, had no pathological alterations in liver morphology, but increased levels of liver cytochrome P450, cytochrome B5, nicotinamide adenine dinucleotide phosphate cytochrome C reductase, aminopyrine N-demethylase, and aniline hydroxylase. (nih.gov)
- A single oral dose of 1·2 or 1·6 ml per kg killed goats within 7 h and resulted in increased activities of aminopyrine N-demethylase and aniline 4-hydroxylase. (elsevier.com)
- No significant changes in P-450 content or in the following activities were noted: ethylmorphine- N - demethylase, lauric acid 1 1-hydroxylation, and lauric acid 12-hydroxylation. (cdc.gov)
- The mean specific activity (A sp ) during 1 h of pre incubation (LMA) of some microsomal mono oxygenases (i.e. ethylmorphine Ndeniethylase, pnitroanisole O-demethylase and aminopyrine Ndemethylase) examined with S9 fractions from sodium phenobarbital and βnaphtho flavone pretreated mice, was doubled when both NADPH and NADH were present. (elsevier.com)
- Likewise, algal diets helped in the process of improvement from liver damage as judged by studying the fluctuation of liver enzymes namely cytochrome P-450, SGPT, G-6-phosphatase and amino-n-demethylase. (scialert.net)
- No significant effects on the activities of aminopyrine N-demethylase and UDP-glucuronyltransferase were observed. (elsevier.com)