Parietal Cells, Gastric
Cytochrome P-450 Enzyme System
A superfamily of hundreds of closely related HEMEPROTEINS found throughout the phylogenetic spectrum, from animals, plants, fungi, to bacteria. They include numerous complex monooxygenases (MIXED FUNCTION OXYGENASES). In animals, these P-450 enzymes serve two major functions: (1) biosynthesis of steroids, fatty acids, and bile acids; (2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs (BIOTRANSFORMATION). They are classified, according to their sequence similarities rather than functions, into CYP gene families (>40% homology) and subfamilies (>59% homology). For example, enzymes from the CYP1, CYP2, and CYP3 gene families are responsible for most drug metabolism.
A group of compounds that contain a bivalent O-O group, i.e., the oxygen atoms are univalent. They can either be inorganic or organic in nature. Such compounds release atomic (nascent) oxygen readily. Thus they are strong oxidizing agents and fire hazards when in contact with combustible materials, especially under high-temperature conditions. The chief industrial uses of peroxides are as oxidizing agents, bleaching agents, and initiators of polymerization. (From Hawley's Condensed Chemical Dictionary, 11th ed)
Lining of the STOMACH, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. The surface cells produce MUCUS that protects the stomach from attack by digestive acid and enzymes. When the epithelium invaginates into the LAMINA PROPRIA at various region of the stomach (CARDIA; GASTRIC FUNDUS; and PYLORUS), different tubular gastric glands are formed. These glands consist of cells that secrete mucus, enzymes, HYDROCHLORIC ACID, or hormones.
Artifactual vesicles formed from the endoplasmic reticulum when cells are disrupted. They are isolated by differential centrifugation and are composed of three structural features: rough vesicles, smooth vesicles, and ribosomes. Numerous enzyme activities are associated with the microsomal fraction. (Glick, Glossary of Biochemistry and Molecular Biology, 1990; from Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Mixed Function Oxygenases
Widely distributed enzymes that carry out oxidation-reduction reactions in which one atom of the oxygen molecule is incorporated into the organic substrate; the other oxygen atom is reduced and combined with hydrogen ions to form water. They are also known as monooxygenases or hydroxylases. These reactions require two substrates as reductants for each of the two oxygen atoms. There are different classes of monooxygenases depending on the type of hydrogen-providing cosubstrate (COENZYMES) required in the mixed-function oxidation.
Rats, Inbred Strains
A megaloblastic anemia occurring in children but more commonly in later life, characterized by histamine-fast achlorhydria, in which the laboratory and clinical manifestations are based on malabsorption of vitamin B 12 due to a failure of the gastric mucosa to secrete adequate and potent intrinsic factor. (Dorland, 27th ed)
Peroxides produced in the presence of a free radical by the oxidation of unsaturated fatty acids in the cell in the presence of molecular oxygen. The formation of lipid peroxides results in the destruction of the original lipid leading to the loss of integrity of the membranes. They therefore cause a variety of toxic effects in vivo and their formation is considered a pathological process in biological systems. Their formation can be inhibited by antioxidants, such as vitamin E, structural separation or low oxygen tension.
A syndrome characterized by recurrent episodes of excruciating pain lasting several seconds or longer in the sensory distribution of the TRIGEMINAL NERVE. Pain may be initiated by stimulation of trigger points on the face, lips, or gums or by movement of facial muscles or chewing. Associated conditions include MULTIPLE SCLEROSIS, vascular anomalies, ANEURYSMS, and neoplasms. (Adams et al., Principles of Neurology, 6th ed, p187)
A generalized seizure disorder characterized by recurrent major motor seizures. The initial brief tonic phase is marked by trunk flexion followed by diffuse extension of the trunk and extremities. The clonic phase features rhythmic flexor contractions of the trunk and limbs, pupillary dilation, elevations of blood pressure and pulse, urinary incontinence, and tongue biting. This is followed by a profound state of depressed consciousness (post-ictal state) which gradually improves over minutes to hours. The disorder may be cryptogenic, familial, or symptomatic (caused by an identified disease process). (From Adams et al., Principles of Neurology, 6th ed, p329)
Recurrent conditions characterized by epileptic seizures which arise diffusely and simultaneously from both hemispheres of the brain. Classification is generally based upon motor manifestations of the seizure (e.g., convulsive, nonconvulsive, akinetic, atonic, etc.) or etiology (e.g., idiopathic, cryptogenic, and symptomatic). (From Mayo Clin Proc, 1996 Apr;71(4):405-14)
Conditions characterized by recurrent paroxysmal neuronal discharges which arise from a focal region of the brain. Partial seizures are divided into simple and complex, depending on whether consciousness is unaltered (simple partial seizure) or disturbed (complex partial seizure). Both types may feature a wide variety of motor, sensory, and autonomic symptoms. Partial seizures may be classified by associated clinical features or anatomic location of the seizure focus. A secondary generalized seizure refers to a partial seizure that spreads to involve the brain diffusely. (From Adams et al., Principles of Neurology, 6th ed, pp317)
Effect of cyclosporine A on cytochrome P-450-mediated drug metabolism in the partially hepatectomized rat. (1/181)Despite its hepatotoxic potential, cyclosporine A (CsA) has been reported to positively influence compensatory liver growth. To probe the physiological consequences of CsA on the recovery of liver function, studies were initiated in the 2/3 partially hepatectomized (PHx) rat, taking the recovery of cytochromes P-450-dependent drug metabolism as primary outcome. CsA was administered at a dose of 3. 33 mg/kg/day for 10 days. Drug metabolism was evaluated by the recovery of 14CO2 after administration of isotopically labeled model drugs and by studying the expression of the P-450 transcripts involved in their biotransformation before and 24 to 96 h after PHx. Before PHx, neither the steady-state mRNA nor the in vivo disposition of caffeine (CYP1A2), erythromycin (CYP3A2 and 3A1), or aminopyrine (CYP2B1 and 2C11) were influenced by CsA. Studies 24 h after PHx revealed a 29 to 39% reduction in the elimination of [14C]aminopyrine and [14C]erythromycin, which was unaffected by CsA. Their metabolism at 48 to 96 h after PHx also remained unaffected by CsA. By contrast, postPHx, [14C]caffeine elimination decreased to a level closely proportional to the loss in liver mass. In addition, CsA accelerated the recovery and/or prevented the decrease of caffeine elimination 24 h after PHx but not at later time points, indicating an early, but unsustained, beneficial effect of CsA on the recovery of CYP1A2-mediated activities. These data show that at the critical time of greatest loss in liver mass, CsA has only a selective influence on the biotransformation of cytochrome P-450 protein-dependent activities and that its effect on the regeneration process does not translate into an overall accelerated recovery of the hepatic drug-metabolizing function. (+info)
L-365,260 inhibits in vitro acid secretion by interacting with a PKA pathway. (2/181)The aim of this study was to analyse the antisecretory mechanism of L-365,260 in vitro in isolated rabbit gastric glands. We showed that compound L-365,260, described as a non-peptide specific competitive CCK-B receptor antagonist, was able to dose-dependently inhibit [14C]-aminopyrine accumulation induced by histamine (10(-4) M), carbachol (5x10(-5) M), 3-isobutyl-1-methyl-xanthine (IBMX) (5x10(-6) M) and forskolin (5x10(-7) M) with similar IC50 values respectively of 1.1+/-0.6x10(-7) M, 1.9+/-1.2x10(-7) M, 4.2+/-2.0x10(-7) M and 4.0+/-2.8x10(-7) M. We showed that L-365,260 acted beyond receptor activation and production of intracellular second messengers and that it had no action on the H+/K+ -ATPase. We found that L-365,260 inhibited cyclic AMP-induced [14C]-aminopyrine accumulation in digitonin-permeabilized rabbit gastric glands, suggesting that this compound acted, at least in part, as an inhibitor of the cyclic AMP-dependent protein kinase (PKA) pathway. (+info)
Demethylation capacity of human fetal adrenal mitochondrial cytochrome P-450 in vitro. (3/181)AIM: To explore the capacity and characteristics of adrenal mitochondria to metabolize xenobiotics in vitro in human fetus. METHODS: Subcellular fractions of fetal adrenal were prepared by differential centrifugation. Mitochondrial P-450 system was proved by spectral analyses and SDS-PAGE. The formaldehyde formation contents were measured with Nash reagent. RESULTS: The erythromycin N-demethylation linearly increased in the protein concentration (1-4 mg)- and incubation time (10-30 min)-dependent manners. A typical concentration-effect relationship appeared with erythromycin 0.067-1 mmol.L-1 and a positive correlation (r = 0.641, P < 0.05) existed between erythromycin N-demethylation and gestation months. The N-demethylation values (nmol.s-1/g protein) of erythromycin (2.7 +/- 0.8), benzfetamine (1.1 +/- 0.5), and aminophenazone (0.9 +/- 0.4) in mitochondria were 89% (P > 0.05), 162% (P < 0.01), and 62% (P < 0.01), respectively, of those in microsomes. There was correlation between mitochondria and microsomes in the N-demethylation of erythromycin (r = 0.708, P < 0.05) and benzfetamine (r = 0.707, P < 0.05). Troleandomycin stimulated erythromycin N-demethylation in adrenal mitochondria as well as in adrenal and liver microsomes in vitro. CONCLUSION: Fetal adrenal mitochondria, with multiple P-450 isoforms and greater capacity of demethylation, play a role in drug-metabolism during fetal development. (+info)
Expression of rab11a N124I in gastric parietal cells inhibits stimulatory recruitment of the H+-K+-ATPase. (4/181)Stimulation of the gastric parietal cell results in a massive redistribution of H+-K+-ATPase from cytoplasmic tubulovesicles to the apical plasma membrane. Previous studies have implicated the small GTPase rab11 in this process. Using matrix-assisted laser desorption mass spectrometry, we confirmed that rab11 is associated with H+-K+-ATPase-enriched gastric microsomes. A stoichiometry of one rab11 per six copies of H+-K+-ATPase was estimated. Furthermore, rab11 exists in at least three forms on rabbit gastric microsomes: the two most prominent resemble rab11a, whereas the third resembles rab11b. Using an adenoviral expression system, we expressed the dominant negative mutant rab11a N124I in primary cultures of rabbit parietal cells under the control of the tetracycline transactivator protein (tTA). The mutant was well expressed with a distribution similar to that of the H+-K+-ATPase. Stimulation of these cultures with histamine and IBMX was assessed by measuring the aminopyrine (AP) uptake relative to resting cells (AP index). In experiments on six culture preparations, stimulated uninfected cells gave an AP index of 10.0 +/- 2.9, whereas parallel cultures expressing rab11a N124I were poorly responsive to stimulation, with a mean AP index of 3.2 +/- 0. 9. Control cultures expressing tTA alone or tTA plus actin responded equally well to stimulation, giving AP index values of 9.0 +/- 3.1 and 9.6 +/- 0.9, respectively. Thus inhibition by rab11a N124I is not simply due to adenoviral infection. The AP uptake data were confirmed by immunocytochemistry. In uninfected cells, H+-K+-ATPase demonstrated a broad cytoplasmic distribution, but it was cleared from the cytoplasm and associated with apically derived membranes on stimulation. In cells expressing rab11a N124I, H+-K+-ATPase maintained its resting localization on stimulation. Furthermore, this effect could be alleviated by culturing infected cells in the presence of tetracycline, which prevents expression of the mutant rab11. We therefore conclude that rab11a is the prominent GTPase associated with gastric microsomes and that it plays a role in parietal cell activation. (+info)
Responsiveness of beta-escin-permeabilized rabbit gastric gland model: effects of functional peptide fragments. (5/181)We established a beta-escin-permeabilized gland model with the use of rabbit isolated gastric glands. The glands retained an ability to secrete acid, monitored by [14C]aminopyrine accumulation, in response to cAMP, forskolin, and histamine. These responses were all inhibited by cAMP-dependent protein kinase inhibitory peptide. Myosin light-chain kinase inhibitory peptide also suppressed aminopyrine accumulation, whereas the inhibitory peptide of protein kinase C or that of calmodulin kinase II was without effect. Guanosine-5'-O-(3-thiotriphosphate) (GTPgammaS) abolished cAMP-stimulated acid secretion concomitantly, interfering with the redistribution of H+-K+-ATPase from tubulovesicles to the apical membrane. To identify the targets of GTPgammaS, effects of peptide fragments of certain GTP-binding proteins were examined. Although none of the peptides related to Rab proteins showed any effect, the inhibitory peptide of Arf protein inhibited cAMP-stimulated secretion. These results demonstrate that our new model, the beta-escin-permeabilized gland, allows the introduction of relatively large molecules, e.g., peptides, into the cell, and will be quite useful for analyzing signal transduction of parietal cell function. (+info)
Regulation and function of p38 protein kinase in isolated canine gastric parietal cells. (6/181)We examined the regulation and functional role of p38 kinase in gastric acid secretion. p38 kinase was immunoprecipitated from cell lysates of highly purified gastric parietal cells in primary culture, and its activity was quantitated by in vitro kinase assay. Carbachol effects were dose- and time-dependent, with a maximal 10-fold stimulatory effect detected after 30 min of incubation. SB-203580, a highly selective inhibitor of p38 kinase, blocked carbachol induction of p38 kinase activity, with maximal inhibition at 10 microM. Stimulation by carbachol was unaffected by preincubation of parietal cells with the intracellular Ca(2+) chelator BAPTA-AM, but incubation of cells in Ca(2+)-free medium led to a 50% inhibition of carbachol induction of p38 kinase activity. Because some of the effects of carbachol are mediated by the small GTP-binding protein Rho, we examined the role of Rho in carbachol induction of p38 kinase activity. We tested the effect of exoenzyme C3 from Clostridium botulinum (C3), a toxin known to ADP-ribosylate and specifically inactivate Rho. C3 led to complete ADP-ribosylation of Rho, and it inhibited carbachol induction of p38 kinase by 50%. We then tested the effect of SB-203580 and C3 on carbachol-stimulated uptake of [(14)C]aminopyrine (AP). Inhibition of p38 kinase by SB-203580 led to a dose-dependent increase in AP uptake induced by carbachol, with maximal (threefold) effect at 10 microM SB-203580. Similarly, preincubation of parietal cells with C3 led to a twofold increase in AP uptake induced by carbachol. Thus carbachol induces a cascade of events in parietal cells that results in activation of p38 kinase through signaling pathways that are at least in part dependent on Rho activation and on the presence of extracellular Ca(2+). p38 kinase appears to inhibit gastric acid secretion. (+info)
Tryptophan operon read-through. Isolation and characterization of an abnormally long tryptophan synthetase alpha subunit from a frame-shift mutant of Escherichia coli. (7/181)A new mutant strain of Escherichia coli, strain ICR-47, contains a frame-shift mutation in the trpA gene, the gene most distal to the operator in the trp operon. Mapping experiments indicate that the lesion is located at a site within 10 to 15% of the end of this gene. The mutation results in "out-of-phase" translation of the distal portion of the trp mRNA; normal translational termination signal(s) are not encountered and a trpA gene product longer than the wild type protein is produced. As with the other enzymes produced from this operon, the in vivo level of the altered protein (the alpha subunit of the tryptophan synthetase enzyme complex) is controlled by exogenous L-tryptophan. The altered alpha subunit from the strain ICR-47 has been isolated and characterized. Molecular weight estimations indicate a molecular weight of approximately 37,000, an increase beyond the wild type enzyme corresponding to an additional 50 to 70 amino acid residues. The protein has a new COOH-terminal amino acid sequence. Results of preliminary hybridization experiments suggest that the ICR-47 mRNA, which is necessarily longer than that needed to code for wild type enzyme, is not detectably different in size from wild type mRNA. The enzymatic properties of the ICR-47 alpha subunit indicates a greatly reduced ability of the mutant subunit to combine functionally with wild type beta2 subunit, the second protein component in the tryptophan synthetase enzyme complex. In contrast, only 40 to 50% of the intrinsic enzymatic activity of the alpha subunit is lost. (+info)
A proposed mechanism for the potentiation of cAMP-mediated acid secretion by carbachol. (8/181)Acid secretion in isolated rabbit gastric glands was monitored by the accumulation of [(14)C]aminopyrine. Stimulation of the glands with carbachol synergistically augmented the response to dibutyryl cAMP. The augmentation persisted even after carbachol was washed out and was resistant to chelated extracellular Ca(2+) and to inhibitors of either protein kinase C or calmodulin kinase II. Cytochalasin D at 10 microM preferentially blocked the secretory effect of carbachol and its synergism with cAMP, whereas it had no effect on histamine- or cAMP-stimulated acid secretion within 15 min. Cytochalasin D inhibited the carbachol-stimulated intracellular Ca(2+) concentration ([Ca(2+)](i)) increase due to release from the Ca(2+) store. Treatment of the glands with cytochalasin D redistributed type 3 inositol 1,4,5-trisphosphate receptor (the major subtype in the parietal cell) from the fraction containing membranes of large size to the microsomal fraction, suggesting a dissociation of the store from the plasma membrane. These findings suggest that intracellular Ca(2+) release by cholinergic stimulation is critical for determining synergism with cAMP in parietal cell activation and that functional coupling between the Ca(2+) store and the receptor is maintained by actin microfilaments. (+info)
Amidopyrine synonyms, amidopyrine antonyms - FreeThesaurus.com
Synonyms for amidopyrine in Free Thesaurus. Antonyms for amidopyrine. 1 synonym for amidopyrine: aminopyrine. What are synonyms for amidopyrine?
Dose dependence of the14C-aminopyrine breath test | SpringerLink
Although the aminopyrine breath test has received much attention, the question has not yet been settled whether pharmacological or tracer doses of the drug should be used. Nine volunteers were given14...
Characterization of oxyntic glands isolated from the rat gastric mucosa
The design of methods useful for the preparation of viable glands and cells from the gastric mucosa allowed detailed studies on the mechanisms that regulate gastric acid secretion. The preparation of rabbit gastric glands was the first suitable method to be used and a number of important scientific contributions have been accomplished with this method. Using this method we studied the effect of CCK-like peptides on [14C]aminopyrine accumulation stimulated by histamine, in order to fmd out whether such peptides can inhibit the production of acid in the parietal cell. We also developed a method for the study of viable rat gastric glands that allowed comparative studies in the rat species.. In rabbit gastric glands CCK-like pep tides inhibited histamine stimulated acid formation whereas gastrin peptides were ineffective. The most potent and efficacious peptides were CCK 8 and the cholecystokinetic amphibian decapeptide cemlein reducing the maximal histamine stimulation of aminopyrine accumulation ...
Aminophenazone - Wikipedia
Aminophenazone (or aminopyrine, amidopyrine, Piramidon) is a pyrazolone with analgesic, anti-inflammatory, and antipyretic properties but has risk of agranulocytosis. A breath test with 13C-labeled aminopyrine has been used as a non-invasive measure of cytochrome P-450 metabolic activity in liver function tests. It is also used in measuring the total body water in the human body system. Production and use have been banned in France, Thailand and India. Symptoms of exposure to this compound include: allergic reactions strong spasmolytic effect on smooth muscle of peripheral blood vessels irritability palsy copious sweating dilated pupils sharp drop then rise in body temperature dysuria dyspnea anxiety tenesmus[disambiguation needed] urinary frequency intermittent fever fatty infiltration of the liver heart muscle degeneration death due to circulatory failure following cardiovascular collapse Agranulocytosis often occurs. Ingestion may cause central nervous system stimulation, vomiting, ...
Dietary restriction of energy and sugar results in a reduction in human cytochrome P450 2E1 activity. | DIAL.pr - BOREAL
Dietary habits are often considered as a pathogenic factor for fatty liver. The impact of dietary intake and steatosis on drug metabolism remains poorly investigated. Our aim was to assess the effect of dietary intake on in vivo cytochrome P450 (CYP) activities in eleven patients with abnormal liver function tests potentially due to fatty liver and associated with a high-sugar diet. Liver function tests, liver volume, aminopyrine breath test (ABT) and chlorzoxazone (CZ) pharmacokinetics (area under the curve, AUC) which are known to reflect CYP2E1 activity were evaluated before and after 2 months restriction of dietary sugar intake. Features at inclusion were an increased BMI (30.3 (SD 3.2) kg/m2), high hepatic volume (1.96 (SD 0.48) litres), hyperechogenic liver parenchyma, elevated liver enzyme activities (alanine aminotransferase (EC 126.96.36.199) 58.6 (SD 17.4) IU/1 with alanine aminotransferase: aspartate aminotransferase (EC 188.8.131.52) ratio , 1), together with a normal ABT value (0.68 (SD 0.21)% ...
VALUE OF AMINOPYRINE*† | Annals of Internal Medicine | American College of Physicians
Modern medical science has made such tremendous strides in the field of therapeutics that many older drugs, especially if potentially productive of untoward and dangerous side-effects, have been discredited and have fallen into disuse. Admittedly, many such drugs have marked therapeutic value and are deserving of further investigation and clinical trial before they are completely discarded.. Aminopyrine, by force of historical circumstances, has been such a discredited drug for many years.1, 2a, 3c, 4b, 5, 6, 7, 8 The danger of agranulocytosis resulting from its use is real and well established, but undoubtedly has been overemphasized.1, 2a, 4b, 5, 6, ...
Aminopyrine Aminophenazone AmidopyrineCAS:58-15-1 - Wuhan Carphetin Chemical Co.,LTD - ecplaza.net
MF:C13H17N3O MW:231.29 CAS:58-15-1 EINECS:200-365-8 Apperance:White fronds crystal or crystalline powder. No smell, taste slightly bitter. Product use: Antipyretic and analgesic, used for fever and headache, joint pain, neuralgia, dysmenorrhea and...
In Vitro Technology - International Society for the Study of Xenobiotics
With that encouragement Axelrod quickly showed that amphetamine was rapidly metabolized in rabbit liver slices. He went on to define the co-factor requirements for the reaction finding that NADPH was required; similar to observations of Bert LaDu in his studies on aminopyrine demethylation9.Axelrod then determined the sub-cellular location of the activity by using methods developed by Hogeboom and Schneider10. This newly defined microsomal oxidizing system was soon shown to be responsible for the metabolism of a wide variety of drugs and other chemicals.. Ref: 1V.R.Potter and C.A.Elvehjem, J.Biol. Chem. ,114:495-504 (1936) 2A.Claude, Cold String Harbor Symposium Quant.Biol.,9:263-270 (1941)3 W.C.Schneider,J.Biol.Chem. 165:585-593 (1949) 4E.C.Miller and J.A.Miller, Cancer Res. 7:468-480 (1947)5 E.S.Stevenson, K. Dobriner, and C.P.Rhoads, Cancer Research 2:160-167 (1942)6 G.C.Mueller and J.A.Miller, J.Biol.. Chem. 176:535-544 (1948) 7G.C.Mueller and J.A. Miller, J.Biol. Chem. ,180:1125-1236 ...
The involvement of a thromboxane (TX) A2-like substance in the decrease of mucosal blood flow (MBF) and occurrence of gastric erosions in rats under water-immersion stress was examined. MBF was estimated by aminopyrine clearance. Stress increased acid output without a parallel increase in MBF and caused erosions. OKY-046, an inhibitor of TXA2 synthesis, and ONO-11120, an antagonist of TXA2 receptors, increased MBF during stress in parallel with an increase in acid output, and erosions did not form. In another experiment, the effects of a TXA2-like substance on MBF during vagal stimulation were examined. Although vagal stimulation alone increased acid output, there were no erosions in the stomach, probably because MBF was increased in parallel with acid output. Intra-arterial administration of a TXA2-like substance formed by the metabolism of arachidonic acid in the blood reduced MBF during vagal stimulation. Intra-arterial administration of ONO-11113, an agonist of TXA2 receptors, also reduced ...
Novodone (Aminophenazone Guaiacolglycolate; Calcium Monoethylcamphorate; Vitamin A...) AGIPS Farmaceutici
Novodone information about active ingredients, pharmaceutical forms and doses by AGIPS Farmaceutici, Novodone indications, usages and related health products lists
BR-4935 Summary Report | CureHunter
BR-4935: cardiotonic; a non-adenosine analog adenosine1-receptor agonist with a substituted 2-thio-3,5-dicyano-4-phenyl-6-aminopyrine
Amidopyrine - Medical Dictionary online-medical-dictionary.org
A pyrazolone with analgesic, anti-inflammatory, and antipyretic properties but has Risk of Agranulocytosis. A Breath Test with 13C-labeled aminopyrine has been used as a non-invasive measure of Cytochrome P-450 metabolic activity in LIVER FUNCTION TESTS ...
The effect of ethanol on drug oxidations in vitro and the significance of ethanol-cytochrome P-450 interaction | Biochemical...
The effect of ethanol on N-demethylation of aminopyrine in rat liver slices and in the microsomal fraction and on microsomal hydroxylation of pentobarbital and aniline was studied. With liver slices N-demethylation of aminopyrine was stimulated by 35-40% at low ethanol concentrations (2mm), whereas no stimulation occurred at high concentrations (100mm). With the liver microsomal fraction, an inhibitory effect was observed only at high ethanol concentrations (100mm). This was also observed with the other drugs studied. In agreement with these results, only at a high concentration did ethanol interfere with the binding of drug substrates to cytochrome P-450. Further, as previously reported, ethanol produced a reverse type I spectral change when added to the liver microsomal fraction. Evidence that this spectral change is due to removal of substrate, endogenously bound to cytochrome P-450, is reported. A dual effect of ethanol is assumed to explain the present findings; in liver slices, at a low ...
COMPARISON OF IN VITRO DRUG METABOLISM BY LUNG, LIVER, AND KIDNEY OF SEVERAL COMMON LABORATORY SPECIES | Drug Metabolism &...
Comparative studies of in vitro drug metabolism by hepatic and extrahepatic tissues have been complicated by the use of a single experimental tissue, few animal species, and variable experimental conditions. In an attempt to minimize these complications, liver, lung and kidney from rat, mouse, rabbit, hamster, and guinea pig were assayed for standard microsomal and soluble fraction enzymes involved in drug biotransformation. For all species, liver was the most active organ. Kidney and lung activities were usually 15%-40% of those found in liver, with kidney slightly more active than lung. No single species demonstrated total superiority in its drug-metabolizing ability, although hamster showed a large number of instances of greatest activity. The rat was a surprisingly poor representative of drug-metabolizing ability; it was superior to the other four species in less than 25% of the instances studied. All species appeared to N-demethylate aminopyrine equally except for high pulmonary and nearly ...
An interdisciplinary exploration of responsible algorithm design in the era of distributed energy resources | Research Square
While the governance of algorithms is of growing societal concern, the energy sector has been slow to engage with this issue. We argue that there are at least three systemic concerns to the design and operation of algorithms in the new, digital energy era. Namely, reliance on algorithms can bi...
Effect of a Campylobacter pylori protein on acid secretion by parietal cells
The uptake of [14C]-aminopyrine by rabbit gastric epithelial cells was used as an indirect assay for acid secretion from parietal cells. Campylobacter pylori strains, isolated from the stomachs of 3 patients with chronic gastritis, duodenal ulcer, and near-normal mucosa, respectively, inhibited acid …
Formic Acid Rubber Pulp Disinfest tianjin - WorldBid B2B Market
Chemicals - Coatings China, Formic Acid Rubber Pulp Disinfest, Uses: 1. Pharmaceutical industry: Caffeine, Enzimes, Aminopyrine, Vitamin B1 2. Pesticide industry: Triazolone, Disinfes...
canine gastric parietal cells Cells | Thermo Fisher Scientific
Maintaining healthy cells is the key to experimental success and reproducible research results. To give you confidence in the health of your cells every step of the way, weve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No matter how you are using your cells, you can count on these products to help keep them healthy.. ...
The effect of a 20-day administration of aminopyrine (600 mg kg-1) as well as two metabolites of aminopyrine, 4-aminoantipyrine (525 mg kg-1) and 4-acetamidoantipyrine (635 mg kg-1), on several hepatic, kidney and serum enzyme activities were investigated. In the aminopyrine-treated group, a pronounced induction of gamma-glutamyl transpeptidase was shown in the whole homogenate as compared to that in the hepatic microsomes. Serum gamma-glutamyl transpeptidase activity was also increased by administration of aminopyrine or 4-aminoantipyrine. No change in gamma-glutamyl transpeptidase activity was observed in kidney and hepatic cytosolic fractions. In all cases, 4-acetamidoantipyrine treatment showed no significant change in the enzyme activities tested. Under the same experimental condition, the amounts of cytochrome P-450 and b5, the activities of aminopyrine N-demethylase, aniline hydroxylase and carboxylesterase of liver microsomes were all induced in the aminopyrine- and ...
Immunology - Immune Response - Barnard Health Care
The gastric autoantigens. The two autoantigens targeted by autoantibodies are components unique to the gastric parietal cell in the. Figure 1 The gastric parietal cell antibody detected by immunofluorescence and illustrated here on murine gastric mucosa reacts with the cytoplasm of gastric parietal cells. Characteristic of the Immunofluorescence reaction is the denser staining of the newly formed cells at the base of the mucosa (bottom left) and lighter staining of the mature cells towards the top of the mucosa. Experimental evidence suggests that the autoantigen in the parietal cell is the enzyme H+,K*-ATPase which is involved in production of acid secreted into the gastric juice.. body of the stomach. The autoantigen with which GPCA reacts is the enzyme H+,K+-adenosine triphosphatase (H+,K+-ATPase) which is cell-specific but not species-specific, indicating the importance of the physiologic role of the molecule in the gastric parietal cell. The second autoantigen is a secreted product of the ...
SMART: Secondary literature for RasGEFN domain
We previously reported that both carbachol and epidermal growth factor (EGF) are potent inducers of the extracellular signal-regulated protein kinases (ERKs) in isolated gastric canine parietal cells and that induction of these kinases leads to acute inhibitory and chronic stimulatory effects on gastric acid secretion. In this study we investigated the molecular mechanisms responsible for these effects. Both carbachol (100 microM) and EGF (10 nM) induced Ras activation. The role of Ras in ERK2 induction was examined by transfecting parietal cells with a vector expressing hemoagglutinin (HA)-tagged ERK2 (HA-ERK2) together with a dominantly expressed mutant (inactive) ras gene. HA-ERK2 activity was quantitated by in-gel kinase assays. Dominant negative Ras reduced carbachol induction of HA-ERK2 activity by 60% and completely inhibited the stimulatory effect of EGF. Since Ras activation requires the assembly of a multiprotein complex, we examined the effect of carbachol and EGF on tyrosyl ...
The blood gastrin curve after histamine stimulation and a protein meal in common diseases of the digestive tract]. - Semantic...
Semantic Scholar extracted view of [The blood gastrin curve after histamine stimulation and a protein meal in common diseases of the digestive tract]. by Francesco Di Mario et al.
Purification and characterization of hepatic P-450IIE1 from acetone-treated mice | DSpace-CRIS DBCF UNISI
A new cytochrome P-450 isozyme (Mr = 52,000) was purified to apparent electrophoretic homogeneity from hepatic microsomes of mice treated with acetone and its biochemical, spectral, and immunological properties characterized. Several criteria indicated that the purified cytochrome was distinct from the known mouse P-450 isozymes. The absolute spectrum of its oxidized form indicated that it was in the high spin state. In a reconstituted system, it showed low catalytic activities towards 7-ethoxycoumarin, aminopyrine, and coumarin, whereas it catalyzed the oxidation of aniline, acetone, dimethylnitrosamine with high turnover number. The mouse enzyme was immunoreactive with polyclonal antibodies against rat P-450IIE1 and exhibited an NH2-terminal aminoacid sequence with a high homology to that of rat-P-450IIE1. Based upon the above catalytic, spectral, immunological and structural properties, the purified mouse P-450 appears to be the ortholog of previously described P-450IIE1(s) of other species ...
Triglyceride - 15-T7532-152 | Pointe Scientific
Serum triglycerides are hydrolyzed to glycerol and free fatty acids by lipase. In the presence of ATP and glycerol kinase (GK), the glycerol is converted to glycerol-1-phosphate. The glycerol-1-phosphate is then oxidized by glycerol phosphate oxidase (GPO) to yield hydrogen peroxide. The condensation of hydrogen peroxide with 4-chlorophenol and 4-aminophenazone (4-AA) in the presence of peroxidase (POD) produces a red colored quinonimine dye which absorbs at, or near 500nm. The intensity of the colored complex formed is directly proportional to the triglycerides concentration of the sample ...
Product Directory on Drugdu.com for Paediatric Compound Sulfamethoxazole Tablets|||206049|||british-mai-worldcom-hebi...
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FDA Advisory No. 2020-2107 || Public Health Warning Against the Purchase and Use of the Following Unregistered Drug Products: -...
The Food and Drug Administration (FDA) advises the public against the purchase and use of the following unregistered drug products: 1. Compound Aminophenazone and Barbital Injection 2mL 2. Calcium Gluconate […]
Parietal cell - Stock Image F002/0341 - Science Photo Library
A stylized view of a parietal cell. Parietal cells are found in the proximal portions of the gastric glands of the stomach and secrete HCl and intrinsic factor. - Stock Image F002/0341
Proper gastric gland | definition of proper gastric gland by Medical dictionary
Looking for online definition of proper gastric gland in the Medical Dictionary? proper gastric gland explanation free. What is proper gastric gland? Meaning of proper gastric gland medical term. What does proper gastric gland mean?
False-colour SEM of gastric glands of stomach - Stock Image P510/0042 - Science Photo Library
Gastric gland: false-colour scanning electron micrograph (SEM) of the mucous membrane lining the stomach, showing the shadowy entrances to a number of gastric glands. The glands secrete gastric juice (hydrochloric acid & the enzyme pepsin) and a protective mucous, derived from the simple, columnar cells (moss-like) that comprise the mucosa. Gastric glands are formed by simple invagination or tubular ingrowth of this mucosa into the underlying layer of tissue, the lamina propria. Magnification: x300 at 6x7cm size. - Stock Image P510/0042
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This is probably a long-awaited update, but I assure you that this is not the last change prepared by us for the next 3 months. Let us know if everything with the new limit works as intended. Cheers ...
Open Call for Patient Group Input - Collaborative Assessment on a Medical Device for Rectum Spacers for Prostate Cancer...
EUnetHTA recently started a new Collaborative Assessment on a Medical Device for rectum spacers for prostate cancer radiotherapy.. To find out about participation, please read more here.. Input submissions will be received through EOB, Friday 5th July.. ...
class=kwd-title|Keywords: von Willebrand Disease Congenital Heart Defects Children Hemorrhage Acquired - Stem cell...
class=kwd-title>Keywords: von Willebrand Disease Congenital Heart Defects Children Hemorrhage Acquired Copyright notice and Disclaimer The publishers final edited version of this article is available at Haemophilia Acquired von Willebrand Syndrome (AVWS) is a bleeding disorder resulting from an acquired deficiency or dysfunction of von Willebrand factor (VWF). stress-induced increase in VWF proteolysis . Diagnosing AVWS can be challenging. Amidopyrine Laboratory assessment needs documentation of the abnormality in the patients VWF. Nevertheless many pre-analytical and individual factors make a difference the sensitivity from the tests useful for diagnosing the problem. Moreover Amidopyrine the variation in assay thresholds and techniques useful for interpreting test outcomes increases the complexity of diagnosing AVWS. In addition due to the rarity of the problem as well as the challenges mixed up in laboratory assessment a higher degree of medical suspicion is necessary by healthcare ...
Metabolism and toxicity of various xenobiotics in vitamin B1 deficiency and after administration of thiamine and thiamine...
Abstract: Metabolism of aminopyrine, sodium benzoate and toxicity of cyclophosphamide were studied in 185 male rats under conditions of various content of vitamin B1 in the animals. Deficiency of thiamin led to an increase in excretion of 4-aminoantipyrine and especially of its acetylated derivative. After administration of thiamin metabolism of aminopyrine was not distinctly altered, while thiamin diphosphate inhibited the drug biotransformation. In deficiency of vitamin B1 transformation of benzoic acid into hippuric acid was inhibited but formation of glucuronides was elevated. Administration of thiamin or thiamin diphosphate stimulated the benzoic acid conjugation and inhibited the glucuronides formation. Deficiency of vitamin B1 accelerated the cyclophosphamide toxicity. Preadministration of thiamin and especially of thiamin diphosphate decreased the toxic effect of cyclophosphamide ...
Monoclonal conversion in human gastric glands gives insights into stem cell and clonal architecture - Experimental Medicine...
Dipyrone - Mechanism, Indication, Contraindications, Dosing, Adverse Effect, Interaction, Hepatic Dose | Drug Index | Pediatric...
Medical information for Dipyrone on Pediatric Oncall including Mechanism, Indication, Contraindications, Dosing, Adverse Effect, Interaction, Hepatic Dose.
Ipl Acne Therapy Treatment | Spirits List
How does IPL work for acne?. There are three forms of IPL therapy for acne - LHE Therapy, Blue-Light Therapy and IPL with medication. The choice will depend on what results youre looking for and what your dermatologist suggests as the most effective for your individual acne spots. LHE therapy uses both heat and light as the source to treat acne. The FDA has actually approved one IPL treatment that involves green light waves and heat. This form of IPL is meant to treat mild to moderate acne and more than likely, over a period of time. The whole process has been known to reduce the size of oil glands and kill the dirt bacteria in pores that create acne.. Blue-Light therapy is similar to LHE minus the UV radiation. It only makes sense that less UV radiation equals less damage on your skin. This form of IPL will have the same effect on killing the dirt bacteria but with less side effects than the LHE therapy. According to Robin McClain, who is a registered nurse practitioner from the University of ...
Alteration of drug metabolizing enzymes in sulphite oxidase deficiency | AVESİS
The aim of this study was to investigate the possible effects of sulphite oxidase (SOX, E.C. 184.108.40.206) deficiency on xenobiotic metabolism. For this purpose, SOX deficiency was produced in rats by the administration of a low molybdenum diet with concurrent addition of 200 ppm tungsten to their drinking water. First, hepatic SOX activity in deficient groups was measured to confirm SOX deficiency. Then, aminopyrine N-demethylase, aniline 4-hydroxylase, aromatase, caffeine N-demethylase, cytochrome b5 reductase, erythromycin N-demethylase, ethoxyresorufin O-deethylase, glutathione S-transferase, N-nitrosodimethylamine N-demethylase and penthoxyresorufin O-deethylase activities were determined to follow changes in the activity of drug metabolizing enzymes in SOX-deficient rats. Our results clearly demonstrated that SOX deficiency significantly elevated A4H, caffeine N-demethylase, erythromycin N-demethylase and N-nitrosodimethylamine N-demethylase activities while decreasing ethoxyresorufin ...
Biotransformation of some pyrazole derivatives to glutathione conjugates in rat liver subcellular fractions.
During incubation of antipyrine, but not amidopyrine, 4-aminoantipyrine and 4-leucylaminoantipyrine, with rat liver microsomaland cytosol fractions in the presence of NADPH-generating system a reactive metabolite, which binds with glutathione is form
Dipyrone - Biology-Online Dictionary
Home » Dipyrone. Dipyrone (Science: chemical) ((2,3-dihydro-1,5-dimethyl-3-oxo-2-phenyl-1h-pyrazol-4-yl)methylamino)methanesulfonic acid. A drug that has analgesic, anti-inflammatory, and antipyretic properties. It is the sodium sulfonate of aminopyrine. Because of the risk of serious adverse effects its use is justified only in serious situations where no alternative is available or suitable. Pharmacological action: non-steroidal anti-inflammatory agents. chemical name: Methanesulfonic acid, ((2,3-dihydro-1,5-dimethyl-3-oxo-2-phenyl-1H-pyrazol-4-yl)methylamino)-, sodium salt ...
Ultrastructural and morphometric features of the acetylcholine innervation in adult rat parietal cortex: An electron...
TY - JOUR. T1 - Ultrastructural and morphometric features of the acetylcholine innervation in adult rat parietal cortex. T2 - An electron microscopic study in serial sections. AU - Umbriaco, Denis. AU - Watkins, Kenneth C.. AU - Descarries, Laurent. AU - Cozzari, Costantino. AU - Hartman, Boyd K. PY - 1994/10/15. Y1 - 1994/10/15. N2 - This study was aimed at characterizing the ultrastructural morphology of the normal acetylcholine (ACh) innervation in adult rat parietal cortex. After immunostaining with a monoclonal antibody against purified rat brain choline acetyltransferase (ChAT), more than 100 immunoreactive axonal varicosities (terminals) from each layer of the Par 1 area were photographed and examined in serial thin sections across their entire volume. These varicosities were relatively small, averaging 0.6 μm in diameter, 1.6 μM2 in surface, and 0.12 μM3 in volume. In every layer, a relatively low proportion exhibited a synaptic membrane differentiation (10% in layer I, 14% in II-III, ...
Surfer on Acid Swizzle Recipe | Serious Eats
The Surfer on Acid is most often consumed in shot form. This variation is a long drink, made extra-refreshing with coconut water.
Hormones and Neurotransmitters Regulating the Secretion of Gastric Acid - Contrast Media
The acid-secretingoxyntic cell, or parietal cell, I is the main cell type in the gastric gland. Chief , cells pepsinogen-secretingcells , mucus cells, s and
A&P Lab Flashcards
the secretion of HCl and digestive enzymes by the gastric glands of the stomach.. contractions of the muscular layer of the stomach wall to increase mixing of the stomach contents. ...
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Anemia, hematinic deficiencies, hyperhomocysteinemia, and serum gastric parietal cell antibody positivity in atrophic glossitis...
TY - JOUR. T1 - Anemia, hematinic deficiencies, hyperhomocysteinemia, and serum gastric parietal cell antibody positivity in atrophic glossitis patients with or without microcytosis. AU - Chiang, Chun Pin. AU - Yu-Fong Chang, Julia. AU - Wang, Yi Ping. AU - Wu, Yang Che. AU - Wu, Yu Hsueh. AU - Sun, Andy. PY - 2019/1/1. Y1 - 2019/1/1. N2 - Background/Purpose: Microcytosis is defined as having mean corpuscular volume (MCV) , 80 fL. This study evaluated whether 79 atrophic glossitis (AG) patients with microcytosis and 985 AG patient without microcytosis had higher frequencies of anemia, hematinic deficiencies, hyperhomocysteinemia, and serum gastric parietal cell antibody (GPCA) positivity than 532 healthy control subjects. Methods: Complete blood count, serum iron, vitamin B12, folic acid, homocysteine, and serum GPCA levels in 79 AG patients with microcytosis, 985 AG patient without microcytosis, and 532 healthy control subjects were measured and compared. Results: We found that 69.6%, 43.0%, ...
Clotrimazole, an N-substituted imidazole widely used as an antifungal agent, has been shown to both inhibit and induce hepatic cytochrome P-450 and related monooxygenase activities. In this study the profile of hepatic cytochrome P-450 isozyme(s) induced by clotrimazole treatment of male Sprague-Dawley rats was investigated. Clotrimazole administration (100 mg/kg, daily for 4 days, ig) resulted in 86% induction of spectrally detectable cytochrome P-450 in hepatic microsomes. In these microsomes 7-ethoxycoumarin O-deethylase (126%), aminopyrine N-demethylase (176%), benzphetamine N-demethylase (117%), p-nitrophenol hydroxylase (89%), and 7-ethoxyresorufin O-deethylase (62%) activities were significantly induced, whereas aryl hydrocarbon hydroxylase activity remained unchanged. Characterization of cytochrome P-450 isozyme(s) in hepatic microsomes prepared from clotrimazole-treated animals was based on the immunoreactivity of these microsomes with highly specific monoclonal antibodies (MAbs) raised ...
Biochemical, histopathological and ultrastructural changes in rat liver induced by r-( + )-pulegone, a monoterpene ketone -...
Biochemical, histopathological and ultrastructural changes occurring at different time points after intraperitoneal administration of a single dose of pulegone (300 mg/kg) were studied. Significant decreases in the level of liver microsomal cytochrome P-450 (67%), heme (37%), aminopyrine N-demethylase (60%) and glucose-6-phosphatase (58%), were noticed 24 hr after pulegone treatment. Alanine amino transferase (ALT) levels increased in a time dependent manner, following exposure of rats to pulegone. Light microscopic studies of liver tissues showed dilation of central veins and distention of sinusoidal spaces 6 hr after pulegone treatment. Initial centrilobular necrosis was noticed at 12 hr. Centrilobular necrosis became severe at 18 hr and nuclear changes included karyorrhexis and karyolysis. Midzonal and periportal degenerative changes in addition to centrilobular necrosis was observed 24 hr after pulegone administration. Electron microscopic changes showed severe degeneration of endoplasmic ...
Ink4a/Arf-Dependent Loss of Parietal Cells Induced by Oxidative Stress Promotes CD44-Dependent Gastric Tumorigenesis | Cancer...
Gastrointestinal malignancies are strongly linked to chronic inflammation (25-29). Gastric tumorigenesis in K19-Wnt1/C2mE mice was also recently shown to be suppressed by knockout of either TNFα or its receptor TNFR1, suggesting that TNFα-dependent inflammation plays a key role in the development of gastric cancer (30). Furthermore, the development of SPEM in response to acute parietal cell loss is dependent on the recruitment of macrophages to the gastric epithelium (31). Together, these findings suggest that inflammation triggers the histopathologic alterations of gastric epithelium that leads to tumor development. We have now shown that the inflammation-associated ROS plays a key role in the parietal cell loss that is an early and critical step in the development of SPEM, and the subsequent formation of gastric tumors. The accumulation of ROS triggers the p38MAPK-p16INK4a/p19ARF signaling pathway selectively in parietal cells and the consequent induction of parietal cell loss. In fact, ...
Unscramble aniline | Words unscrambled from letters aniline | Scrabble Word aniline | Words Made with the Letters aniline
Unscramble aniline, Unscramble letters aniline, Point value for aniline, Word Decoder for aniline, Word generator using the letters aniline, Word Solver aniline, Possible Scrabble words with aniline, Anagram of aniline
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Ilya B. Tsyrlov
Tsyrlov, I. B.; Gerasimov, K. E. (1991). "Aminopyrine-N-demethylase. I. Directed modification of substrates' structure as a way ...
... (or aminopyrine, amidopyrine, Pyramidon) is a non-narcotic analgesic substance. It is a pyrazolone and a ... A breath test with 13C-labeled aminopyrine has been used as a non-invasive measure of cytochrome P-450 metabolic activity in ... Caubet MS, Laplante A, Caillé J, Brazier JL (June 2002). "[13C]aminopyrine and [13C]caffeine breath test: influence of gender, ... Panchal, J (29 January 2021). "A Review: Why Aminopyrine Banned?" (PDF). Scholars Academic Journal of Pharmacy. "Aminophenazone ...
Aminopyrine Hart FD, Boardman PL (June 1964). "Trial of Nifenazone ("Thylin")". British Medical Journal. 1 (5397): 1553-4. doi: ...
In 1897 he invented "Pyramidon", the trade name for aminopyrine. With Arthur Stoll, he is credited with the isolation of ...
... is a metabolite of aminopyrine with analgesic, anti-inflammatory, and antipyretic properties. Its use as a drug is ...
In 1893, a derivative of antipyrine, aminopyrine, was made by Friedrich Stolz at Hoechst.: 26-27 Yet later, chemists at Hoechst ...
Imaoka S, Inoue K, Funae Y (1988). "Aminopyrine metabolism by multiple forms of cytochrome P-450 from rat liver microsomes: ... simultaneous quantitation of four aminopyrine metabolites by high-performance liquid chromatography". Arch. Biochem. Biophys. ...
... hexachlorobiphenyl found increased activation of ethoxyresorufin deethylase and aminopyrine demethylase. However, while also ...
List of MeSH codes (D03)
... aminopyrine MeSH D03.383.129.539.850.077.025 - ampyrone MeSH D03.383.129.539.850.077.150 - dipyrone (metamizole) MeSH D03.383. ...
... or aminopyrine PNU-91356A (U-91356) This set index page lists chemical structure articles associated with the same molecular ...
List of MeSH codes (D08)
... aminopyrine n-demethylase MeSH D08.811.682.662.582.338 - cytochrome p-450 cyp2e1 MeSH D08.811.682.662.582.353 - cytochrome p- ...
13C-aminopyrine breath test to evaluate severity of disease in patients with chronic hepatitis C virus infection<...
Breath samples were collected every 30 min up to 2 h after 13C-aminopyrine administration. 13C-Aminopyrine breath test results ... Breath samples were collected every 30 min up to 2 h after 13C-aminopyrine administration. 13C-Aminopyrine breath test results ... Breath samples were collected every 30 min up to 2 h after 13C-aminopyrine administration. 13C-Aminopyrine breath test results ... Breath samples were collected every 30 min up to 2 h after 13C-aminopyrine administration. 13C-Aminopyrine breath test results ...
IMSEAR at SEARO: Analgesics. V. Pyrazolon derivatives: antipyrine (phenazone), aminopyrine (pyramidon), and phenylbutazone ...
Table of Contents - May 01, 1992, 20 (3) | Drug Metabolism & Disposition
Food and Drug Regulations
WHO EMRO | Pharmacoepidemiological study of self-medication in adults attending pharmacies in Alexandria, Egypt | Volume 15,...
Frontiers | Geraniol Pharmacokinetics, Bioavailability and Its Multiple Effects on the Liver Antioxidant and Xenobiotic...
Aminopyrine N-demethylase (APND) activity-CYP3A1/2. Activity was determined by the quantification of CH2O release, according to ... The total incubation volume was 3 ml, composed of 0.5 ml water solution of 50 mM aminopyrine and 25 mM MgCl2, 1.48 ml of 0.60 ... Incubation mixture consisted of 2.6 ml, composed of 1 mM ethoxycoumarin, 5 mM MgCl2, NADPH-generating system (see aminopyrine ... Acetonitrile (PubChem CID:6342), aminopyrine (PubChem CID:6009), bovine serum albumin, dichlorophenolindophenol (PubChem CID: ...
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Kevin Fish | Stanford Medicine
The activity rate of aminopyrine N-demethylase was not changed by the diet; however, p-nitroanisole O-demethylase activity was ... The activity rate of aminopyrine N-demethylase was not changed by the diet; however, p-nitroanisole O-demethylase activity was ... The activity rate of aminopyrine N-demethylase was not changed by the diet; however, p-nitroanisole O-demethylase activity was ...
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Food and Drug Regulations
Aminopyrine and Dipyrone. C.01.131 No person shall sell Aminopyrine or Dipyrone (a derivative of Aminopyrine) for oral or ... C.01.133 The provisions of sections C.01.131 and C.01.132 do not apply to preparations containing Aminopyrine or Dipyrone that ... Bearing in mind the possibility that such reactions may occur, Aminopyrine or Dipyrone should be used only when other less ... "WARNING: Fatal agranulocytosis may be associated with the use of Aminopyrine and Dipyrone. It is essential that adequate blood ...
Aminopyrine - Preferred Concept UI. M0000965. Scope note. A pyrazolone with analgesic, anti-inflammatory, and antipyretic ... Aminopyrine Entry term(s). Amidazophen Amidophen Amidophenazon Amidopyrine Aminofenazone Aminophenazone Dimethyl N ... A breath test with 13C-labeled aminopyrine has been used as a non-invasive measure of CYTOCHROME P-450 metabolic activity in ... A breath test with 13C-labeled aminopyrine has been used as a non-invasive measure of CYTOCHROME P-450 metabolic activity in ...
Neutropenia: Practice Essentials, Background, Pathophysiology
- V. Pyrazolon derivatives: antipyrine (phenazone), aminopyrine (pyramidon), and phenylbutazone (butazolidin). (who.int)
- Comparison with aminopyrine N-demethylase activity. (aspetjournals.org)
- no significant changes were noted in liver aminopyrine - N - demethylase or aniline hydrolase activities. (cdc.gov)
- Oral administration of kopsinine 200 mg/kg once daily for 3 d significantly increased liver microsomal protein, cytochrome P-450, cytochrome b5, NADPH-cytochrome C reductase, aminopyrine demethylase and benzo(a)pyrine hydroxylase activities in mice. (chinaphar.com)
- Metyrapon, a specific inhibitor of cytochrome P-450, partially antagonized aminopyrine demethylase activity of microsomes from mice treated with kopsinine. (chinaphar.com)
- From in vitro experiments, all three had been found to be potent inhibitors of oxidation of Kind-I substrates (ethylmorphine and aminopyrine) and less potent, non-competitive inhibitors of Sort-II substrates (aniline and dimethylnitrosamine). (findnolvadex.com)
- Background: There are few data on the use of the 13 C-aminopyrine breath test to evaluate the severity of disease in patients with hepatitis C virus-related chronic liver disease, although these patients represent one of the most important problems in clinical hepatology. (elsevier.com)
- Aims: To compare 13 C-aminopyrine breath test results of patients with hepatitis C virus-related chronic hepatitis and Child-Pugh class A cirrhosis with those of normal subjects, and to evaluate different methods of expressing 13 C-aminopyrine breath test results. (elsevier.com)
- Methods: Twenty-four patients with hepatitis C virus-related chronic hepatitis and 17 patients with Child-Pugh class A cirrhosis underwent 13 C-aminopyrine breath test. (elsevier.com)
- 13 C-Aminopyrine breath test results were expressed as a percentage of the administered dose of 13 C recovered per hour (% dose/h) and the cumulative percentage of administered dose of 13 C recovered over time (% dose cum). (elsevier.com)
- The fibrosis score showed a significant inverse correlation with the 13 C-aminopyrine breath test result (% dose/h) at 30 min (rs = - 0.409, P = 0.05). (elsevier.com)
- The 13 C-aminopyrine breath test results (% dose cum) at 30. (elsevier.com)
- The 13 C-aminopyrine breath test result (% dose cum) was also able to distinguish between normal subjects and chronic hepatitis patients with high but not low fibrosis scores. (elsevier.com)
- Both 13 C-aminopyrine breath test results (% dose/h and % dose cum) at 120 min allowed the differentiation between normal subjects and chronic hepatitis patients with high (≥ 6) necro-inflammatory activity. (elsevier.com)
- Conclusions: In patients with hepatitis C virus-related chronic liver disease, the 13 C-aminopyrine breath test proved to be safe and easy to perform, and was able to evaluate different degrees of liver function impairment which were partly correlated to clinical and histological evaluation. (elsevier.com)
- In future studies, 13 C-aminopyrine breath test results should be expressed in a standardized fashion to permit comparison. (elsevier.com)
- A breath test with 13C-labeled aminopyrine has been used as a non-invasive measure of CYTOCHROME P-450 metabolic activity in LIVER FUNCTION TESTS . (bvsalud.org)
- Breath samples were collected every 30 min up to 2 h after 13 C-aminopyrine administration. (elsevier.com)
- Isoproterenol (Iso), epinephrine and norepinephrine each stimulated isolated gastric mucosal parietal cells as shown by an increased accumulation of [14C]aminopyrine (AP), an indirect measure of acid secretion. (aspetjournals.org)
- The aminopyrine breath test has been shown to be a sensitive noninvasive indicator of liver cell dysfunction. (nih.gov)
- In a search for a noninvasive method of monitoring the effects of methotrexate therapy, we have investigated the use of the aminopyrine breath test in patients receiving methotrexate for the treatment of severe psoriasis. (nih.gov)
- The [14C]-aminopyrine breath test was performed in 20 normal control subjects, 32 patients with psoriasis receiving methotrexate therapy, and 8 patients with histologically confirmed cirrhosis of differing etiology. (nih.gov)
- The aminopyrine breath test was only able to detect the later severe stages of methotrexate hepatotoxicity, grade III, when fibrosis occurs, before established cirrhosis was present. (nih.gov)
- Our data suggests that the aminopyrine breath test is not a sensitive indicator for the detection of early methotrexate-induced hepatotoxicity, (stages I and II), but will detect the precirrhotic stage III change. (nih.gov)
- Consequently, we recommend that a liver biopsy should be performed annually in all psoriatic patients receiving methotrexate, to detect histological damage, especially when the aminopyrine breath test score falls below the 95% confidence limits of normal. (nih.gov)
- 7. Aminopyrine breath test predicts liver-related events and death in HCV-related cirrhosis on SVR after DAA therapy. (nih.gov)
- A breath test with 13C-labeled aminopyrine has been used as a non-invasive measure of CYTOCHROME P-450 metabolic activity in LIVER FUNCTION TESTS . (nih.gov)
- To determine whether Type II diabetes or glyburide affect hepatic drug metabolism, the authors used 13 C‐labeled aminopyrine and caffeine breath tests as in vivo probes of the hepatic cytochrome P450 and P 1 450 enzyme activities respectively in six subjects with Type II diabetes (4 women, 2 men). (northwestern.edu)
- The caffeine and aminopyrine breath tests (CBT, ABT) were performed sequentially while fasting before the start of glyburide treatment (5 mg daily) and at weekly intervals for 4 weeks. (northwestern.edu)
- Type II diabetes that is poorly controlled by diet alone is not associated with alterations of the hepatic drug metabolizing enzymes as judged by the caffeine and aminopyrine breath tests. (northwestern.edu)
- 13 C-Aminopyrine breath test to evaluate severity of disease in patients with chronic hepatitis C virus infection. (rackfish.net)
- 1) The 14C-aminopyrine breath test was used to measure liver function in 14 normal subjects, 16 patients with alcoholic cirrhosis, 14 alcoholics without cirrhosis, and 29 patients taking a variety of drugs. (comparewords.com)
- And because ginseng is very difficult to produce, it may also have sugar, caffeine, alcohol, phenylbutazone, aminopyrine and other substandard herbs just to "fluff up" their product.Our organic ginseng powder contains pure, unadulterated ginseng. (impactfoods.co.uk)
- Both cryoprotectant media were equally efficient at preserving enzyme activities in the hepatocytes over 7 days at -80 degrees C. Cytochrome P450 and reduced glutathione content and the activities of the microsomal enzymes responsible for aminopyrine N-demethylation and epoxide hydrolysis were well maintained over 7 days storage. (nih.gov)
- Brodie and Axelrod continued to collaborate on a number of projects related to the metabolism of analgesic drugs including phenacetin, antipyrine, aminopyrine, and dicoumerol. (nih.gov)
- 1968). Eugenol had no effect on aminopyrine-N-demethylation activity in the liver of rats given about 10% of the LD 50 three times daily for 2-2/3 days. (inchem.org)
- Kinetics of 13C02 exhaled by rats after [~3C2]-aminopyrine in normal condi- tions (Basal) and 5 rain after administration of a dose of 164 mgkg of diethyldithiocar- bamate. (findbinaryoption.com)
- 5 ]. It also allows pharmaceutical compa- tional allocation technique, where 30 en- nies to expand their market [ 6 ]. (who.int)
- PubChem Compound Summary for CID 6009, Aminopyrine. (vibratist.com)
- https://pubchem.ncbi.nlm.nih.gov/compound/Aminopyrine. (vibratist.com)
- Quinidine sulfate is heat resistant or sensitive to oxidation, 7 ml of the discovered since aminopyrine. (thehasse.org)