The smooth muscle cell. III. Elastin synthesis in arterial smooth muscle cell culture. (1/87)
Primate arterial smooth muscle cells and skin fibroblasts were examined for their ability to synthesize elastin in culture. In the presence of the lathyrogen beta-aminopropionitrile, the smooth muscle cells incorporate [3H]lysine into a lysyl oxidase substrate that was present in the medium and associated with the cell layer. A component having a mol wt of 72,000 and an electrophoretic mobility similar to that of authentic tropoelastin was isolated from the labeled smooth muscle cells by coacervation and fractionation with organic solvents. In the absence of beta-aminopropionitrile, long-term cultures of smooth muscle cells incorporated [14C]lysine into desmosine and isodesmosine, the cross-link amino acids unique to elastin. In contrast, no desmosine formation occurred in the fibroblast cultures. These characteristics demonstrate that arterial smooth muscle cells are capable of synthesizing both soluble and cross-lined elastin in culture. (+info)Lysyl oxidase activates the transcription activity of human collagene III promoter. Possible involvement of Ku antigen. (2/87)
Lysyl oxidase is an extracellular enzyme that controls the maturation of collagen and elastin. Lysyl oxidase and collagen III often show similar expression patterns in fibrotic tissues. Therefore, we investigated the influence of lysyl oxidase overexpression on the promoter activity of human COL3A1 gene. Our results showed that when COS-7 cells overexpressed the mature form of lysyl oxidase, the activity of the human COL3A1 promoter was increased up to an average of 12 times when tested by luciferase reporter assay. The effect was specific, because other promoters were not affected. Moreover, lysyl oxidase effect was abolished by beta-aminopropionitrile, a specific inhibitor of its catalytic activity. Electrophoretic mobility shift assay showed a binding activity in the region from -101 to -77 that was significantly increased by lysyl oxidase overexpression. The binding was specifically competed by the cold probe, and the mutagenesis of this region abolished both the binding activity in gel retardation and lysyl oxidase stimulation of COL3A1 promoter in transfection experiments. We identified the binding activity as Ku antigen in its two components: Ku80 and Ku70. This study suggests a new coordinated mechanism by which lysyl oxidase might control the development of fibrosis. (+info)Collagen synthesis in capsules surrounding dimethylbenzanthracene-induced rat breast tumors and the effect of pretreatment with beta-aminopropionitrile. (3/87)
Collagen synthesis is increased over three-fold in capsules surrounding dimethylbenzanthracene-induced rat breast tumors compared to the tumor parenchyma and over six-fold compared to normal breast connective tissue. Increased collagen synthesis is independent of the rate of tumor growth and final tumor size. Pretreatment of animals with beta-aminopropionitrile to inhibit collagen cross-linking caused an 82% decrease in tumor formation and a significant reduction in tumor volume (approximately 0.4 cu cm) compared to controls (approximately 10 cu cm). The four small tumors that did develop in the lathyritic animals had increased collagen synthesis in the interior tumor stroma and reduced collagen synthesis in the tumor capsule. These findings suggest that the collagenous capsule surrounding dimethylbenzanthracene tumors functions as a physical barrier to protect the tumor from the immune system of the host. The apparent antitumor effects of beta-aminopropionitrile may be due to immunopotentiation and/or cytotoxic actions of the drug. (+info)Analysis of the combined osteolathyritic effects of beta-aminopropionitrile and diethyldithiocarbamate on xenopus development. (4/87)
In order to examine the mechanistic basis between combined effects and mechanisms of action, two osteolathyrogens, beta-aminopropionitrile (betaAPN) and diethyldithiocarbamate (DTC), were tested together on Xenopus embryos. In a separate test, DTC was also tested with copper sulfate to determine the importance of copper in DTC-induced osteolathyrism. Frog embryos (Xenopus laevis) were exposed for 96 h, with daily solution removal and replacement. Preserved tadpoles were evaluated for osteolathyritic lesions. For the betaAPN:DTC test, a 1.2-factor matrix design was used, producing two single chemical and seven mixture-response curves. The chi(2) goodness-of-fit test was used to compare the experimental mixture-response curves with theoretical effects for two combined effects models, dose-addition and independence. All seven mixture curves were consistent with expected results for dose-addition, but the correlations were generally not high. For the DTC:copper test, the three mixture-response curves generated showed that added copper increased the DTC-alone EC(50), but there was no corresponding right shift at the top of the response curves, as observed previously with betaAPN and copper. In the betaAPN:DTC and DTC:copper tests, DTC alone showed a biphasic concentration-osteolathyrism curve, and the slope of the response curve for DTC alone in each test was statistically different than the slope for the betaAPN alone response curve. Taken together, the results suggest the potential for a second osteolathyritic effect of DTC that affected the combined toxicity enough to produce a dose-addition correlation without the chemicals necessarily having the same mechanism. (+info)Amine oxidase-like activity of polyphenols. Mechanism and properties. (5/87)
Polyphenols in several oxidation systems gained amine oxidase-like activity, probably due to the formation of the corresponding quinones. In the presence of Cu(II), o- and p-phenolic compounds exhibited amine oxidase-like activity, whereas only the o-phenolic compounds showed the activity in the presence of 1,1-diphenyl-2-picrylhydrazyl radical. The activity was determined by measuring the conversion of benzylamine to benzaldehyde by HPLC. Moreover, gallic acid, chlorogenic acid, and caffeic acid, which are plant polyphenols, converted the lysine residue of bovine serum albumin to alpha-amino-adipic semialdehyde residue, indicating lysyl oxidase-like activity. We also characterized the activity of pyrocatechol, hydroquinone, and pyrogallol in the presence of Cu(II). The oxidative deamination was accelerated at a higher pH, and required O2 and transition metal ions. Furthermore, EDTA markedly inhibited the reaction but not beta-aminopropionitrile, which is a specific inhibitor of lysyl oxidase. Catalase significantly inhibited the oxidation, implying the participation of hydroxyl radical in the reaction, but superoxide dismutase stimulated the oxidation, probably due to its radical formation activity. We discussed the mechanism of the oxidative deamination by polyphenols and the possible significance of the activity for biological systems. (+info)The effect of beta aminoproprionitrile (BAPN) on experimental amyloidosis. (6/87)
Experimental amyloidosis was induced in mice with repeated injections of complete Freund's adjuvant (CFA) reinforced with bacterial vaccine. BAPN administered in a mixture with CFA or on its own before the injection of CFA reduced the incidence of amyloidosis. The reduction in the incidence of amyloidosis following the administration of BAPN may be due to its inhibitory effect on the oxidative deamination of amino acids, which presumably inhibit cross-linking of amyloid fibrils or interfere with metabolic pathways which involve the formations of mucopolysaccharide formation. It is suggested that the defective formation of the mucopolysaccharide-amyloid protein complex inhibits amyloid deposition and induces the activity of beta glucuronidase observed in the present study. The reduced incidence of amyloidosis following BAPN adminsitration cannot be due to lysosomal enzyme degradation of the amyloid as the activity of cathepsin D and acid phosphatase is decreased during this process. (+info)A molecular role for lysyl oxidase in breast cancer invasion. (7/87)
We identified previously an up-regulation in lysyl oxidase (LOX) expression,an extracellular matrix remodeling enzyme, in a highly invasive/metastatic human breast cancer cell line, MDA-MB-231, compared with MCF-7, a poorly invasive/nonmetastatic breast cancer cell line. In this study, we demonstrate that the mRNA expression of LOX and other LOX family members [lysyl oxidase-like (LOXL), LOXL2, LOXL3, and LOXL4] was observed only in breast cancer cells with a highly invasive/metastatic phenotype but not in poorly invasive/nonmetastatic breast cancer cells. LOX and LOXL2 showed the strongest association with invasive potential in both highly invasive/metastatic breast cancer cell lines tested (MDA-MB-231 and Hs578T). To determine whether LOX is directly involved in breast cancer invasion, LOX antisense oligonucleotides were transfected into MDA-MB-231 and Hs578T cells, and found to inhibit invasion through a collagen IV/laminin/gelatin matrix in vitro compared with LOX sense oligonucleotide-treated and untreated controls. In addition, treatment of MDA-MB-231 and Hs578T cells with beta-aminopropionitrile (an irreversible inhibitor of LOX enzymatic activity) decreased invasive activity. Conversely, MCF-7 cells transfected with the murine LOX gene demonstrated a 2-fold increase in invasiveness that was reversible by the addition of beta-aminopropionitrile in a dose-dependent manner. In addition, endogenous LOX mRNA expression was induced when MCF-7 cells were cultured in the presence of fibroblast conditioned medium or conditioned matrix, suggesting a role for stromal fibroblasts in LOX regulation in breast cancer cells. Moreover, the correlation of LOX up-regulation and invasive/metastatic potential was additionally demonstrated in rat prostatic tumor cell lines, and human cutaneous and uveal melanoma cell lines. These results provide substantial new evidence that LOX is involved in cancer cell invasion. (+info)An angiotensin-converting enzyme inhibitor, not an angiotensin II type-1 receptor blocker, prevents beta-aminopropionitrile monofumarate-induced aortic dissection in rats. (8/87)
OBJECTIVE: Cystic medial degeneration (CMD) is a histologic abnormality that is common in aortic diseases such as aortic dilation, aneurysm, or dissection. Although little is known about the mechanism underlying CMD, we have previously demonstrated that angiotensin II signaling via angiotensin II type 2 receptor (AT2R) plays a central role in apoptosis of vascular smooth muscle cells (VSMCs) occurring in CMD associated with Marfan syndrome. The aim of this study is to elucidate the role of angiotensin II signaling in THE pathogenesis of aortic diseases associated with CMD. METHOD: We investigated the effects of angiotensin-converting enzyme inhibitor (ACEI), temocapril (n = 15), angiotensin II receptor type-1 (AT1R) blocker, CS-866 (n = 15), and vehicle control (n = 17) on 0.25% beta-aminopropionitrile monofumarate (BAPN)-induced aortic dissection and histopathologic findings in a rat model. RESULTS: Temocapril significantly prevented aortic dissection (P <.05), CMD (P <.01), and VSMC apoptosis (P <.01) compared with vehicle control in BAPN-fed rats. However, CS-866 did not show any preventive effect. Reversed transcriptase-polymerase chain reaction demonstrated that expression of both AT1R and AT2R was detected in control rat aortas, and that AT2R expression was significantly upregulated in the aortas of BAPN-fed rats (P <.01). Blood pressure was significantly and equally lowered in both temocapril and CS-866 groups compared with control. CONCLUSIONS: Differential expression of angiotensin II receptors and AT2R signaling are involved in the pathogenesis of CMD and aortic dissection in BAPN-fed rats. ACEIs might be of clinical value for the prevention and treatment of aortic diseases related to CMD. (+info)Effects of beta-aminopropionitrile, L-azetidine-2-carboxylic acid and beta-xyloside on the tunica media of the developing chick...
JAIRO | ß-aminopropionitrile投与による鶏胚骨格異常の解析
Bioline International Official Site (site up-dated regularly)
GMD - [1647] 3-aminopropionitrile 2 [9.927]
Effects of induction and inhibition of matrix cross-linking on remodeling of the aqueous outflow resistance by ocular...
Allylamine and β-aminopropionitrile induced aortic medial necrosis: Mechanisms of synergism<...
EXPERIMENTAL LATHYRISM | JCB
Anti-LOX / Lysyl Oxidase Antibody | Rabbit anti-Human Polyclonal | LSBio
Patent US5120322 - Method and apparatus for treatment of fibrotic lesions - Google Patents
Plus it
Studies Show That Olive Oil Benefits Our Heart and Mind
Salt-soluble elastin from lathyritic chicks | Biochemical Journal
The role of lysyl oxidase in SRC-dependent proliferation and metastasis of colorectal cancer<...
Protein-lysine 6-oxidase
Patent US7208300 - Member of the lysyl oxidase gene family - Google Patents
LOX propeptide - NB110-41568B | acris-antibodies.com
Histochemical Evidence of Aldehyde Blocking Capacity of Lathyrogenic Agents<...
A dual-cell device designed as an oxidase mimic and its use for the study of oxidase-like nanozymes - Chemical Communications ...
Matrix cross-linking lysyl oxidases are induced in response to myocardial infarction and promote cardiac dysfunction
Lysyl Oxidase-Like 2 (LOXL2) ELISA Kits
anti Lysyl Oxidase Like 3 LOXL3 - Journal for the International Association for Laboratory Learning and Technology
Plus it
The role of lysyl oxidase-like 1 DNA copy number variantsin exfoliation glaucoma
Lysyl oxidase‐like 2 represses Notch1 expression in the skin to promote squamous cell carcinoma progression | The EMBO Journal
Poster Presentation - Events 4 You Limited
Pearl Izumi Elite Gel FF Cycling Glove - Mens - Backed by a 100% Satisfaction Guarantee | TriVillage.com
Lathyrism : Wheeless Textbook of Orthopaedics
Hypoxia-induced lysyl oxidase is a critical mediator of bone marrow cell recruitment to form the premetastatic niche
Osteopontin-mediated myocardial fibrosis in heart failure: a role for lysyl oxidase?
Recombinant Human LOXL2 Protein, His-tagged LOXL2-272H - Creative BioMart
LOX Enzyme May Be Scleroderma Marker, Therapeutic Target, Study Says
Human bone collagen synthesis is a rapid, nutritionally modulated process<...
Medical Advisor Journals, Kyle J. Norton Site, Health Tips for Better Living and Living Health: Hormones
Fabrication of oxidase-like hollow MnCo2O4 nanofibers and their sensitive colorimetric detection of sulfite and l-cysteine -...
Lysyl oxidase interacts with AGEs signaling to modulate collagen synthesis in polycystic ovarian tissue
LOXL1 Polymorphism in Pseudoexfoliation Syndrome - Full Text View - ClinicalTrials.gov
What is meant by staggered ends in collagen molecules?
Aminopropionitrile
... , also known as β-aminopropionitrile (BAPN), is an organic compound with both amine and nitrile functional ... Beta-amino-propionitrile (BAPN) found in lathyrus odoratus (common garden sweet pea) is thought to be responsible for ... Aminopropionitrile is prepared by the reaction of ammonia with acrylonitrile. Kashin-Beck disease Lysyl oxidase Marfan syndrome ... "Aminopropionitrile - Compound Summary". PubChem Compound. USA: National Center for Biotechnology Information. 25 March 2005. ...
Lathyrism
It is caused by a different toxin, beta-aminopropionitrile, which affects the linking of the subunits of collagen, a major ... It is also caused by the toxin beta-aminopropionitrile. Eating the grasspea with legumes having high concentrations of sulphur- ... employing the toxin beta-aminopropionitrile. The blood vessels are affected, as opposed to bone. Neurolathyrism is caused by ...
1,3-Diaminopropane
It is prepared by the amination of acrylonitrile followed by hydrogenation of the resulting aminopropionitrile. The potassium ...
Cartilage
... modulation of balance between proteoglycan and collagen network in vitro with beta-aminopropionitrile". Osteoarthritis and ...
Sheila MacNeil
They went on to explore how aminopropionitrile in sweet peas could be bound with polymers, and created CryoSkin. They were ...
Neurolathyrism
It is caused by a different toxin (beta-aminopropionitrile) which affects the linking of collagen, a protein of connective ... The cause of this disease is attributed to beta-aminopropionitrile, which inhibits the copper-containing enzyme lysyl oxidase, ... It is a skeletal disorder, caused by the toxin beta-aminopropionitrile (BAPN), and characterized by hernias, aortic dissection ...
Osteolathyrism
The most widely studied of these compounds is beta-aminopropionitrile (BAPN), which exerts its deleterious effect by an unknown ... beta-aminopropionitrile), which affects the linking of collagen, a protein of connective tissues. The condition results in ...
Angiolathyrism
The main chemical responsible is β-Aminopropionitrile, which prevents collagen cross-linking, thus making the blood vessel, ...
Abdominal aortic aneurysm
A recent study has shown that β-Aminopropionitrile plus elastase application to abdominal aorta causes more severe aneurysm in ...
Sweet pea
Seeds of the sweet pea contain beta-aminopropionitrile that prevents the cross-linking of collagen by inhibiting lysyl oxidase ...
ATC code M01
... combinations QM01AX90 Pentosan polysulfate QM01AX91 Aminopropionitrile QM01AX92 Grapiprant QM01AX99 Combinations M01BA01 ...
Controlled Drugs and Substances Act
... amino]propionitrile) and any salt thereof Mefenorex (d,l-N-(3-chloropropyl)-α-methylbenzeneethanamine) and any salt thereof ...
C3H6N2
The molecular formula C3H6N2 (molar mass: 70.09 g/mol, exact mass: 70.0531 u) may refer to: Aminopropionitrile (BAPN) ...
Lysyl oxidase
... lysyl oxidase was inhibited either by nutritional copper-deficiency or by supplementation of diets with β-aminopropionitrile ( ...
IMSEAR at SEARO: Effect of topical beta-aminopropionitrile on connective tissue of granulomas.
Inhibition of collagen aggregation in rat cartilage induced by beta amino propionitrile in vitro - Fingerprint
- Research...
Benzodiazepines and Other Targeted Substances Regulations
Human Metabolome Database: HMDB0014918 (Phenelzine) Protein Associations
ALDH2 knockout protects against aortic dissection | BMC Cardiovascular Disorders | Full Text
Knockout of ALDH2 mitigated β-aminopropionitrile-induced TAD formation in animal studies. Ultrasound results showed that ALDH2 ... T cells and T helper cells were decreased in ALDH2 knockout mice treated with β-aminopropionitrile for 28 days. ALDH2 knockout ... Three-week-old male mice were administered freshly prepared β-aminopropionitrile solution dissolved in drinking water (1 g ... β-aminopropionitrile (BAPN) treatment led to AD formation by inhibiting lysyl oxidase activity [15]. Three-week-old male WT ...
NIOSHTIC-2 Search Results - Full View
Extracellular matrix (ECM) remodelling from the adipose tissue plays a pivotal - Stem cell application on skin cancer research
Modulation of the Secretory Pathway Rescues Zebrafish Polycystic Kidney Disease Pathology | American Society of Nephrology
DeCS
Code System Concept
Piroxicam - Wikipedia
Piroxicam is a nonsteroidal anti-inflammatory drug (NSAID) of the oxicam class used to relieve the symptoms of painful inflammatory conditions like arthritis.[3][4] Piroxicam works by preventing the production of endogenous prostaglandins which are involved in the mediation of pain, stiffness, tenderness and swelling.[3] The medicine is available as capsules, tablets and (not in all countries) as a prescription-free gel 0.5%.[5] It is also available in a betadex formulation, which allows a more rapid absorption of piroxicam from the digestive tract.[3] Piroxicam is one of the few NSAIDs that can be given parenteral routes.[citation needed] It was patented in 1968 by Pfizer and approved for medical use in 1979.[6] It became generic in 1992,[7] and is marketed worldwide under many brandnames.[1] ...
Effect of lysyl oxidase inhibition on angiotensin ii-induced arterial hypertension, remodeling, and stiffness - Fingerprint ...
Pesquisa | Portal Regional da BVS
DeCS 2016 - June 12, 2016 version
Author: Muratoglu, Selen C : Search
The WikiDoc Living Textbook of Allergic Disorders and Immune Modulation - wikidoc
MeSH Browser
FDA Update on Grain-free Diets and Heart Disease in Dogs |
isothiocarbamate | ADHD ADD
GAP-43 | Science and Nuts
S-EPMC6218375 - Detecting intimal tear and subintimal blood flow of thrombosed acute aortic dissection with ulcer-like...
DeCS 2018 - July 31, 2018 version
DeCS 2017 - July 04, 2017 version
DeCS 2016 - June 12, 2016 version
DeCS 2017 - December 21, 2017 version
Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone | Profiles RNS
BAPN2
- For this function, we have examined the effect of -aminopropionitrile (BAPN), a particular and irreversible inhibitor of LOX activity, inside a style of diet-induced weight problems. (exposed-skin-care.net)
- In obese rats, the inhibition of LOX activity through -aminopropionitrile (BAPN, a particular inhibitor of LOX activity) decreases adipose cells fibrosis, partly corrects the adipocyte-size distribution design (moving it toward smaller sized sizes) and attenuates the upsurge in bodyweight and extra fat mass. (exposed-skin-care.net)
Beta-aminopropionitrile2
- IMSEAR at SEARO: Effect of topical beta-aminopropionitrile on connective tissue of granulomas. (who.int)
- For example, Riley et al have prevented fibrosis in animal models by administering either proline analogues, which interfere with proper folding of collagen, or beta-aminopropionitrile, which interferes with collagen crosslinking. (cdc.gov)
Irreversible inhibitor1
- Because collagens and elastin are important components of the extracellular matrix, abnormalities in their modification can be expected to affect many tissues, as seen in lathyrism, a connective tissue disorder caused by the administration of ß-aminopropionitrile, an irreversible inhibitor of lysyl oxidases. (adhd-npf.com)
Lysyl2
- The most promising method seems to be the blocking of crosslinks formation among collagen molecules by β-aminopropionitrile, a competitive inhibitor of a crosslinking enzyme, lysyl oxidase. (nih.gov)
- Because collagens and elastin are important components of the extracellular matrix, abnormalities in their modification can be expected to affect many tissues, as seen in lathyrism, a connective tissue disorder caused by the administration of ß-aminopropionitrile, an irreversible inhibitor of lysyl oxidases. (adhd-npf.com)
Rats1
- Establishment and effect evaluation of an aortic dissection model induced by different doses of β-aminopropionitrile in rats. (medscape.com)
Molecules1
- The emission of 2-aminopropionitrile was modeled simultaneously with the emission of all molecules known in Sgr B2(N), which allowed us to properly take into account line blending and avoid misassignments. (u-strasbg.fr)
Searches1
- Rotational spectrum of a chiral amino acid precursor, 2-aminopropionitrile, and searches for it in Sagittarius B2 (N). (u-strasbg.fr)