Reagent used as an intermediate in the manufacture of beta-alanine and pantothenic acid.

The smooth muscle cell. III. Elastin synthesis in arterial smooth muscle cell culture. (1/87)

Primate arterial smooth muscle cells and skin fibroblasts were examined for their ability to synthesize elastin in culture. In the presence of the lathyrogen beta-aminopropionitrile, the smooth muscle cells incorporate [3H]lysine into a lysyl oxidase substrate that was present in the medium and associated with the cell layer. A component having a mol wt of 72,000 and an electrophoretic mobility similar to that of authentic tropoelastin was isolated from the labeled smooth muscle cells by coacervation and fractionation with organic solvents. In the absence of beta-aminopropionitrile, long-term cultures of smooth muscle cells incorporated [14C]lysine into desmosine and isodesmosine, the cross-link amino acids unique to elastin. In contrast, no desmosine formation occurred in the fibroblast cultures. These characteristics demonstrate that arterial smooth muscle cells are capable of synthesizing both soluble and cross-lined elastin in culture.  (+info)

Lysyl oxidase activates the transcription activity of human collagene III promoter. Possible involvement of Ku antigen. (2/87)

Lysyl oxidase is an extracellular enzyme that controls the maturation of collagen and elastin. Lysyl oxidase and collagen III often show similar expression patterns in fibrotic tissues. Therefore, we investigated the influence of lysyl oxidase overexpression on the promoter activity of human COL3A1 gene. Our results showed that when COS-7 cells overexpressed the mature form of lysyl oxidase, the activity of the human COL3A1 promoter was increased up to an average of 12 times when tested by luciferase reporter assay. The effect was specific, because other promoters were not affected. Moreover, lysyl oxidase effect was abolished by beta-aminopropionitrile, a specific inhibitor of its catalytic activity. Electrophoretic mobility shift assay showed a binding activity in the region from -101 to -77 that was significantly increased by lysyl oxidase overexpression. The binding was specifically competed by the cold probe, and the mutagenesis of this region abolished both the binding activity in gel retardation and lysyl oxidase stimulation of COL3A1 promoter in transfection experiments. We identified the binding activity as Ku antigen in its two components: Ku80 and Ku70. This study suggests a new coordinated mechanism by which lysyl oxidase might control the development of fibrosis.  (+info)

Collagen synthesis in capsules surrounding dimethylbenzanthracene-induced rat breast tumors and the effect of pretreatment with beta-aminopropionitrile. (3/87)

Collagen synthesis is increased over three-fold in capsules surrounding dimethylbenzanthracene-induced rat breast tumors compared to the tumor parenchyma and over six-fold compared to normal breast connective tissue. Increased collagen synthesis is independent of the rate of tumor growth and final tumor size. Pretreatment of animals with beta-aminopropionitrile to inhibit collagen cross-linking caused an 82% decrease in tumor formation and a significant reduction in tumor volume (approximately 0.4 cu cm) compared to controls (approximately 10 cu cm). The four small tumors that did develop in the lathyritic animals had increased collagen synthesis in the interior tumor stroma and reduced collagen synthesis in the tumor capsule. These findings suggest that the collagenous capsule surrounding dimethylbenzanthracene tumors functions as a physical barrier to protect the tumor from the immune system of the host. The apparent antitumor effects of beta-aminopropionitrile may be due to immunopotentiation and/or cytotoxic actions of the drug.  (+info)

Analysis of the combined osteolathyritic effects of beta-aminopropionitrile and diethyldithiocarbamate on xenopus development. (4/87)

In order to examine the mechanistic basis between combined effects and mechanisms of action, two osteolathyrogens, beta-aminopropionitrile (betaAPN) and diethyldithiocarbamate (DTC), were tested together on Xenopus embryos. In a separate test, DTC was also tested with copper sulfate to determine the importance of copper in DTC-induced osteolathyrism. Frog embryos (Xenopus laevis) were exposed for 96 h, with daily solution removal and replacement. Preserved tadpoles were evaluated for osteolathyritic lesions. For the betaAPN:DTC test, a 1.2-factor matrix design was used, producing two single chemical and seven mixture-response curves. The chi(2) goodness-of-fit test was used to compare the experimental mixture-response curves with theoretical effects for two combined effects models, dose-addition and independence. All seven mixture curves were consistent with expected results for dose-addition, but the correlations were generally not high. For the DTC:copper test, the three mixture-response curves generated showed that added copper increased the DTC-alone EC(50), but there was no corresponding right shift at the top of the response curves, as observed previously with betaAPN and copper. In the betaAPN:DTC and DTC:copper tests, DTC alone showed a biphasic concentration-osteolathyrism curve, and the slope of the response curve for DTC alone in each test was statistically different than the slope for the betaAPN alone response curve. Taken together, the results suggest the potential for a second osteolathyritic effect of DTC that affected the combined toxicity enough to produce a dose-addition correlation without the chemicals necessarily having the same mechanism.  (+info)

Amine oxidase-like activity of polyphenols. Mechanism and properties. (5/87)

Polyphenols in several oxidation systems gained amine oxidase-like activity, probably due to the formation of the corresponding quinones. In the presence of Cu(II), o- and p-phenolic compounds exhibited amine oxidase-like activity, whereas only the o-phenolic compounds showed the activity in the presence of 1,1-diphenyl-2-picrylhydrazyl radical. The activity was determined by measuring the conversion of benzylamine to benzaldehyde by HPLC. Moreover, gallic acid, chlorogenic acid, and caffeic acid, which are plant polyphenols, converted the lysine residue of bovine serum albumin to alpha-amino-adipic semialdehyde residue, indicating lysyl oxidase-like activity. We also characterized the activity of pyrocatechol, hydroquinone, and pyrogallol in the presence of Cu(II). The oxidative deamination was accelerated at a higher pH, and required O2 and transition metal ions. Furthermore, EDTA markedly inhibited the reaction but not beta-aminopropionitrile, which is a specific inhibitor of lysyl oxidase. Catalase significantly inhibited the oxidation, implying the participation of hydroxyl radical in the reaction, but superoxide dismutase stimulated the oxidation, probably due to its radical formation activity. We discussed the mechanism of the oxidative deamination by polyphenols and the possible significance of the activity for biological systems.  (+info)

The effect of beta aminoproprionitrile (BAPN) on experimental amyloidosis. (6/87)

Experimental amyloidosis was induced in mice with repeated injections of complete Freund's adjuvant (CFA) reinforced with bacterial vaccine. BAPN administered in a mixture with CFA or on its own before the injection of CFA reduced the incidence of amyloidosis. The reduction in the incidence of amyloidosis following the administration of BAPN may be due to its inhibitory effect on the oxidative deamination of amino acids, which presumably inhibit cross-linking of amyloid fibrils or interfere with metabolic pathways which involve the formations of mucopolysaccharide formation. It is suggested that the defective formation of the mucopolysaccharide-amyloid protein complex inhibits amyloid deposition and induces the activity of beta glucuronidase observed in the present study. The reduced incidence of amyloidosis following BAPN adminsitration cannot be due to lysosomal enzyme degradation of the amyloid as the activity of cathepsin D and acid phosphatase is decreased during this process.  (+info)

A molecular role for lysyl oxidase in breast cancer invasion. (7/87)

We identified previously an up-regulation in lysyl oxidase (LOX) expression,an extracellular matrix remodeling enzyme, in a highly invasive/metastatic human breast cancer cell line, MDA-MB-231, compared with MCF-7, a poorly invasive/nonmetastatic breast cancer cell line. In this study, we demonstrate that the mRNA expression of LOX and other LOX family members [lysyl oxidase-like (LOXL), LOXL2, LOXL3, and LOXL4] was observed only in breast cancer cells with a highly invasive/metastatic phenotype but not in poorly invasive/nonmetastatic breast cancer cells. LOX and LOXL2 showed the strongest association with invasive potential in both highly invasive/metastatic breast cancer cell lines tested (MDA-MB-231 and Hs578T). To determine whether LOX is directly involved in breast cancer invasion, LOX antisense oligonucleotides were transfected into MDA-MB-231 and Hs578T cells, and found to inhibit invasion through a collagen IV/laminin/gelatin matrix in vitro compared with LOX sense oligonucleotide-treated and untreated controls. In addition, treatment of MDA-MB-231 and Hs578T cells with beta-aminopropionitrile (an irreversible inhibitor of LOX enzymatic activity) decreased invasive activity. Conversely, MCF-7 cells transfected with the murine LOX gene demonstrated a 2-fold increase in invasiveness that was reversible by the addition of beta-aminopropionitrile in a dose-dependent manner. In addition, endogenous LOX mRNA expression was induced when MCF-7 cells were cultured in the presence of fibroblast conditioned medium or conditioned matrix, suggesting a role for stromal fibroblasts in LOX regulation in breast cancer cells. Moreover, the correlation of LOX up-regulation and invasive/metastatic potential was additionally demonstrated in rat prostatic tumor cell lines, and human cutaneous and uveal melanoma cell lines. These results provide substantial new evidence that LOX is involved in cancer cell invasion.  (+info)

An angiotensin-converting enzyme inhibitor, not an angiotensin II type-1 receptor blocker, prevents beta-aminopropionitrile monofumarate-induced aortic dissection in rats. (8/87)

OBJECTIVE: Cystic medial degeneration (CMD) is a histologic abnormality that is common in aortic diseases such as aortic dilation, aneurysm, or dissection. Although little is known about the mechanism underlying CMD, we have previously demonstrated that angiotensin II signaling via angiotensin II type 2 receptor (AT2R) plays a central role in apoptosis of vascular smooth muscle cells (VSMCs) occurring in CMD associated with Marfan syndrome. The aim of this study is to elucidate the role of angiotensin II signaling in THE pathogenesis of aortic diseases associated with CMD. METHOD: We investigated the effects of angiotensin-converting enzyme inhibitor (ACEI), temocapril (n = 15), angiotensin II receptor type-1 (AT1R) blocker, CS-866 (n = 15), and vehicle control (n = 17) on 0.25% beta-aminopropionitrile monofumarate (BAPN)-induced aortic dissection and histopathologic findings in a rat model. RESULTS: Temocapril significantly prevented aortic dissection (P <.05), CMD (P <.01), and VSMC apoptosis (P <.01) compared with vehicle control in BAPN-fed rats. However, CS-866 did not show any preventive effect. Reversed transcriptase-polymerase chain reaction demonstrated that expression of both AT1R and AT2R was detected in control rat aortas, and that AT2R expression was significantly upregulated in the aortas of BAPN-fed rats (P <.01). Blood pressure was significantly and equally lowered in both temocapril and CS-866 groups compared with control. CONCLUSIONS: Differential expression of angiotensin II receptors and AT2R signaling are involved in the pathogenesis of CMD and aortic dissection in BAPN-fed rats. ACEIs might be of clinical value for the prevention and treatment of aortic diseases related to CMD.  (+info)

Post a Comment for Effects of beta-aminopropionitrile, L-azetidine-2-carboxylic acid and beta-xyloside on the tunica media of the developing chick aorta : a mongraphic study by Susan Averill Taylor. ...
ß-aminopropionitrile投与による鶏胚骨格異常の解析ß-aminopropionitrile投与による鶏胚骨格異常の解析 ...
Administration of an antifibrotic agent as an adjunct to antihelmintic treatment with the objective of morbidity reduction was investigated in the murine schistosomiasis mansoni model. Antifibrotic, beta-aminopropionitrile treatment has a profound effect on the cellular matrix composition of the liver granuloma of Schistosoma mansoni infected mice when given alone, resulting in increase macrophage infiltration. These macrophages, in response to stimulation with soluble egg antigen or lipopolysaccharide produced elevated levels of nitric oxide but low levels of tumor necrosis factor alpha compared to untreated infected mice. This also correlated with reduced liver granuloma size. In spite of low numbers of eggs in the liver, mice receiving a combine treatment had a high level of resistance to a challenge infection compared with mice receiving only praziquantel. Those mice also exhibited a reduced lymphocyte proliferative response, similar to that of infected untreated mice. Antifibrotic treatment ...
The Golm Metabolome Database (GMD) facilitates the search for and dissemination of mass spectra from biologically active metabolites quantified using GC-MS.
TY - JOUR. T1 - Effects of induction and inhibition of matrix cross-linking on remodeling of the aqueous outflow resistance by ocular trabecular meshwork cells. AU - Yang, Yong Feng. AU - Sun, Ying Ying. AU - Acott, Ted. AU - Keller, Kate. PY - 2016/7/28. Y1 - 2016/7/28. N2 - The trabecular meshwork (TM) tissue controls drainage of aqueous humor from the anterior chamber of the eye primarily by regulating extracellular matrix (ECM) remodeling by matrix metalloproteinases (MMPs). Glaucomatous TM tissue is stiffer than age-matched controls, which may be due to alterations in ECM cross-linking. In this study, we used genipin or beta-Aminopropionitrile (BAPN) agents to induce or inhibit matrix cross-linking, respectively, to investigate the effects on outflow resistance and ECM remodeling. Treatment with BAPN increased outflow rates in perfused human and porcine anterior segments, whereas genipin reduced outflow. Using a fluorogenic peptide assay, MMP activity was increased with BAPN treatment, but ...
TY - JOUR. T1 - Allylamine and β-aminopropionitrile induced aortic medial necrosis. T2 - Mechanisms of synergism. AU - Kumar, D.. AU - Trent, M. B.. AU - Boor, P. J.. PY - 1998/2/6. Y1 - 1998/2/6. N2 - We have developed a model of aortic smooth muscle necrosis in adult Sprague Dawley rats by feeding them two vascular toxins (allylamine HCl, or AA, and β-aminopropionitrile, or βAPN) in concert for 10 days. Either toxin given alone does not cause aortic lesions. In order to shed light on the mechanism of the synergistic action of these two toxins we fed known modulators of AA or βAPN toxicity to rats concurrently with the two toxins. As modulators we used (a) semicarbazide (98 mg/kg/day, given 4 h prior to toxins), a known inhibitor of the vascular enzyme SSAO which metabolizes AA (b) L-cysteine (1.5% in rat chow, beginning 3 days prior to toxins), which has been shown to reduce the toxic effects of βAPN; and (c) phenelzine sulphate (3 mg/kg/day, given 4 h prior to toxins), an inhibitor of ...
In normal and lathyritic chick embryos bone collagen was synthesized primarily in the periosteum of the femurs, and was organized as radioactive spicules in these bones. Saline extraction of the lathyritic bones removed the radioactive spicules, although they eventually seemed to become non-extractable. Normal bone seemed to be unaffected by saline extraction. Marked variation in the degree of isotope incorporation was seen in collagenous and non-collagenous tissues. All the tissues of any one embryo, however, showed a similar degree of isotope incorporation. Tritiated ß-aminopropionitrile was diffusely distributed throughout bone and was completely removed by saline extraction. This autoradiographic study supports the postulate that a portion of extractable lathyritic collagen is recently synthesized and is organized in fibrous structures in bone.. ...
Lysyl Oxidase antibody LS-C141074 is a DY488-conjugated rabbit polyclonal antibody to human Lysyl Oxidase (LOX) (aa300-400). Validated for IHC and WB.
A method of controlling scar formation in humans by applying a force to tissue in an amount to create a wound; applying a lathyrogenic agent to the wound; forming the wound into a predetermined stationary or moveable beneficial configuration; and maintaining the wound in the predetermined configuration and in contact with lathyrogenic agent during healing.
The transcriptional activator IFN regulatory factor 1 (IRF-1) and its antagonistic repressor IRF-2 are regulators of the IFN system. IRF-1 also manifests tumor suppressive activity, and its inactivation could contribute to the development of human hematopoietic malignancies. Here, we report the identification of the lysyl oxidase gene as a target gene of IRF-1. An IRF response element was identified in the lysyl oxidase gene promoter. We also demonstrate that the transformed phenotype of ras-expressing embryonic fibroblasts with a null mutation in the IRF-1 allele could be suppressed by the expression of the lysyl oxidase cDNA, implicating its potential role in tumor suppression. Thus, the regulation of the lysyl oxidase gene by IRF-1 could contribute to the multistep process of malignant transformation.. ...
Olive oil is packed with a large number of phenolic compounds, which are the antioxidants that give the oil its pungent, bitter taste. According to an in-depth review published in Nutrition Research Reviews in June 2005, the most powerful of these compounds, such as hydroxytyrosol and oleuropein, can guard us from serious diseases like cancer, attack destructive microorganisms in our body and regulate the immune system.. Moreover, a study published in ACS Chemical Neuroscience in 2013 showed that another phenolic compound in olive oil, called oleocanthal, had neuroprotective properties and could shield us from Alzheimers disease, which is a leading cause of dementia. These results might explain why the Mediterranean diet, which uses olive oil freely, is associated with better cognitive function and lower rates of cognitive decline.. ...
The isolation of a salt-soluble homogeneous elastin from the aortas of lathyritic chicks by chromatography on DEAE-cellulose and salt precipitation is described. These new techniques, as well as some previously published by other workers, were evaluated with the help of antiserum raised in sheep against insoluble chick elastin. The purified elastin was very basic and behaved in a predictable manner in coacervation studies. The protein migrated in sodium dodecyl sulphate-polyacrylamide gels as a single band moving slightly faster than pyruvate kinase (mol.wt. 57000).. ...
TY - JOUR. T1 - The role of lysyl oxidase in SRC-dependent proliferation and metastasis of colorectal cancer. AU - Baker, Ann-Marie. AU - Cox, Thomas R.. AU - Bird, Demelza. AU - Lang, Georgina. AU - Murray, Graeme I.. AU - Sun, Xiao-Feng. AU - Southall, Stacey M.. AU - Wilson, Jon R.. AU - Erler, Janine T.. PY - 2011/3/2. Y1 - 2011/3/2. N2 - Background: Emerging evidence implicates lysyl oxidase (LOX), an extracellular matrix-modifying enzyme, in promoting metastasis of solid tumors. We investigated whether LOX plays an important role in the metastasis of colorectal cancer (CRC). Methods: We analyzed LOX expression in a patient CRC tissue microarray consisting of normal colon mucosa (n = 49), primary (n = 510), and metastatic (n = 198) tissues. LOX was overexpressed in CRC cell line SW480 (SW480+LOX), and the expression was knocked down in CRC cell line SW620 using LOX-specific short hairpin RNA (SW620+shLOX). Effect of LOX manipulation on three-dimensional cell proliferation and invasion was ...
This gene encodes a member of the lysyl oxidase family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate a regulatory propeptide and the mature enzyme. The copper-dependent amine oxidase activity of this enzyme functions in the crosslinking of collagens and elastin, while the propeptide may play a role in tumor suppression. In addition, defects in this gene have been linked with predisposition to thoracic aortic aneurysms and dissections. [provided by RefSeq, Jul 2016 ...
The present invention relates to a novel lysyl oxidase genes, termed EER-7. The invention relates to the protein and nucleic acids encoding the protein. The invention further relates to an assay system to identify compounds that selectively modulate EER-7 protein activity by interaction with estrogen receptors.
LOX propeptide, 0.1 ml. Lysyl oxidase (LOX) is an extracellular enzyme that is critical for the normal biosynthesis of collagens and elastin.
TY - JOUR. T1 - Histochemical Evidence of Aldehyde Blocking Capacity of Lathyrogenic Agents. AU - Yeager, Vernon L.. AU - Sevekson, Arlen R.. PY - 1961/12. Y1 - 1961/12. N2 - This study has demonstrated that compounds known to possess lathyrogenic properties may, or may not, block the periodic acid-Schiff reaction, and that for these compounds, no correlation exists between their aldehyde blocking capacity and their ability to produce lathyrism in tadpoles. The mechanism by which those compounds produce lathyrism therefore continues to be obscure.. AB - This study has demonstrated that compounds known to possess lathyrogenic properties may, or may not, block the periodic acid-Schiff reaction, and that for these compounds, no correlation exists between their aldehyde blocking capacity and their ability to produce lathyrism in tadpoles. The mechanism by which those compounds produce lathyrism therefore continues to be obscure.. UR - ...
A dual-cell device has been designed as an oxidase-like mimic with the oxidation of 3,3′,5,5′-tetramethylbenzidine as a model reaction. This dual-cell device could be also used to study oxidase-like nanozymes. It was found that only the catalytic sites for oxygen reduction are essential and necessary for oxidase-li
AIMS: After myocardial infarction (MI), extensive remodelling of the extracellular matrix contributes to scar formation. While aiming to preserve tissue integrity, this fibrotic response is also associated with adverse events, including a markedly increased risk of heart failure, ventricular arrhythmias, and sudden cardiac death. Cardiac fibrosis is characterized by extensive deposition of collagen and also by increased stiffness as a consequence of enhanced collagen cross-linking. Members of the lysyl oxidase (LOX) family of enzymes are responsible for the formation of collagen cross-links. This study investigates the contribution of LOX family members to the heart response to MI. METHODS AND RESULTS: Experimental MI was induced in C57BL/6 mice by permanent ligation of the left anterior descending coronary artery. The expression of LOX isoforms (LOX and LOXL1-4) was strongly increased upon MI, and this response was accompanied by a significant accumulation of mature collagen fibres in the ...
Reactivity: Chicken, Cow, Human and more. Compare different LOXL2 ELISA Kits & buy the right one directly at antibodies-online.com!
Description: Quantitativesandwich ELISA kit for measuring Human Lysyl oxidase homolog 3(LOXL3) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits ...
Intratumoral collagen cross-links heighten stromal stiffness and stimulate tumor cell invasion, but it is unclear how collagen cross-linking is regulated in epithelial tumors. To address this question, we used KrasLA1 mice, which develop lung adenocarcinomas from somatic activation of a KrasG12D allele. The lung tumors in KrasLA1 mice were highly fibrotic and contained cancer-associated fibroblasts (CAFs) that produced collagen and generated stiffness in collagen gels. In xenograft tumors generated by injection of wild-type mice with lung adenocarcinoma cells alone or in combination with CAFs, the total concentration of collagen cross-links was the same in tumors generated with or without CAFs, but co-injected tumors had higher hydroxylysine aldehyde-derived collagen cross-links (HLCCs) and lower lysine-aldehyde-derived collagen cross-links (LCCs). Therefore, we postulated that an LCC-to-HLCC switch induced by CAFs promotes the migratory and invasive properties of lung adenocarcinoma cells. To ...
LIU Y ; WHIGHAM BT ; WHEELER J ; WILLIAMS SEI ; Rautenbach RM ; Ziskind A ; Ramsay M ; CARMICHAEL TR ; Ashley-Koch AE ; Rand Allingham R ; Hauser M (2012) ...
During the last few years, accumulated evidence has supported a complex and pleiotropic role for proteins of the LOX family. Apart from their classical and widely reported function as ECM remodeling factors (Csiszar, 2001; Lucero & Kagan, 2006), emerging data have demonstrated the involvement of lysyl oxidases in a plethora of intracellular and extracellular processes (Barker et al, 2012; Cano et al, 2012). Generation of loss‐of‐function mouse models for Lox and Loxl1 has shed some light on tissue‐specific functions of these two LOX family members. The prototypic Lox plays a key role in the cardiovascular system (Maki et al, 2002; Hornstra et al, 2003), and Loxl1 was shown to be critical during the elastogenesis process (Liu et al, 2004). However, in vivo data concerning other members of the LOX family remain unexplored although similar multifunctional actions have been described, in particular for LOXL2 (Cano et al, 2012). Therefore, we generated deletion and overexpression mice for Loxl2 ...
Background/Aims: Maintenance of bone quality is important in the prevention of osteoporotic fracture. Bone quality is determined by collagen cross-links which are regulated by enzyme lysyl oxidase (LOX) in osteoblastic cells. LOX expression is known to be inhibited by activation of Janus kinase (JAK) signaling located in the upstream of LOX. But, JAK signaling is reported to be inhibited by Tocotrienol-Rich Fraction (TRF), a member of the vitamin E family. However, the effect of TRF on the LOX expression in osteoblastic cells has not been understood. Here, we have investigated the relation between TRF and LOX expression.. Methods: A human osteosarcoma cell line (MG-63) was cultured in medium containing 5 µg/ml or 10 µg/ml TRF. After 24 h of treatment TRF, we analyzed LOX mRNA expression and JAK1, JAK2 protein expression and activation. Analyses of mRNA expression and of protein expression and activation were performed by real-time PCR and westernblotting respectively. Expression and activation ...
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Orthopaedic Specialists of North Carolina. Dr. Wheeless enjoys and performs all types of orthopaedic surgery but is renowned for his expertise in total joint arthroplasty (Hip and Knee replacement) as well as complex joint infections. He founded Orthopaedic Specialists of North Carolina in 2001 and practices at Franklin Regional Medical Center and Duke Raleigh Hospital.. » More about Dr. Wheeless. ...
Tumor cell metastasis is facilitated by premetastatic niches formed in destination organs by invading bone marrow-derived cells (BMDCs). Lysyl oxidase (LOX) is critical for premetastatic niche formation. LOX secreted by hypoxic breast tumor cells accumulates at premetastatic sites, crosslinks coll …
An excess of OPN is associated with increased LOX and insoluble collagen, as well as with LV stiffness and systolic dysfunction in patients with HHD and HF. In addition, OPN up-regulates LOX in human fibroblasts. It is suggested that the OPN-LOX axis might facilitate the formation of insoluble colla …
Purified Recombinant Human LOXL2 Protein, His-tagged from Creative Biomart. Recombinant Human LOXL2 Protein, His-tagged can be used for research.
The molecule lysyl oxidase promotes skin and lung scarring in scleroderma and may serve as a biomarker and therapeutic target, a study says.
TY - JOUR. T1 - Human bone collagen synthesis is a rapid, nutritionally modulated process. AU - Babraj, John A.. AU - Smith, Kenneth. AU - Cuthbertson, Daniel J.R.. AU - Rickhuss, Peter. AU - Dorling, James S.. AU - Rennie, Michael J.. PY - 2005/6. Y1 - 2005/6. N2 - We developed a direct assay of human bone collagen synthesis using [13C] or [15N] proline and applied it to determine the effects of feeding in young healthy men. Surprisingly, postabsorptive bone collagen synthesis is not sluggish, being ∼0.07%/h more rapid than that of muscle protein, and capable of being stimulated within 4 h of intravenous feeding by 66 ± 13%.Introduction: All current methods for estimation of bone collagen turnover are indirect, depending on the assay of collagen markers. Our aim was to develop a direct method for human bone collagen synthesis to be used to study its physiology and pathology, and specifically, in the first instance, the effect of feeding.Materials and methods: We applied, over 2 h, flooding ...
Histamine, found within granules of basophils and mast cells (,90% of body stores) is a biogenic amine and an organic nitrogen compound that occurs to various degrees in many foods such as cherries to about 0.17-13.46 ng/g, bananas and grapes, rice and cereals, herbs, olive oil, wine, beer, etc.. In healthy persons, dietary histamine can be rapidly detoxified by amine oxidases, whereas persons with low amine oxidase activity are at risk of histamine toxicity(a). the hormone, as a neurotransmitter is involved in regulating physiological function in the gut and immune response to foreign pathogens ...
Mixed transition-metal oxides (MTMOs) have attracted much research interest because of their promising applications in artificial enzymes. In this work, uniform hollow MnCo2O4 nanofibers have been fabricated via an electrospinning technique followed by a calcination process, which can be used as efficient oxidase m
The connective tissue components, collagen types I, III and IV, which surround the ovarian follicles, undergo drastic changes during ovulation. Abnormal collagen synthesis and increased volume and density of ovarian stroma characterise the polycystic ovary syndrome (PCOS). Physiologically, collagen synthesis in ovarian follicles is partly regulated by lysyl oxidase (LOX), which catalyzes the collagen and elastin cross-linking and plays indispensable role in the organization of ovarian extracellular matrix (ECM) during follicular development. We have recently shown accumulation of advanced glycation end products (AGEs), nutritional metabolic products that stimulate ECM production and abnormal collagen cross-linking, in ovarian tissue of patients with PCOS. However, the possible link between LOX and AGEs-induced signaling in collagen assembly and stroma formation remain elusive. The present investigation explores the hypothesis that AGE-mediated signaling affects LOX gene transcription in ovarian ...
To evaluate the association profiles of the lysyl oxidase-like 1 gene polymorphisms with pseudoexfoliation syndrome in the Korean population, peripheral blood sampling will be done from the patients with pseudoexfoliation.. And genotypes of the three single nucleotide polymorphisms of lysyl oxidase-like 1 gene , rs1048661, rs3825942, rs2165241 were analyzed by direct sequencing. ...
Covalent cross-links hold many collagen molecules side by side, forming fibres.The ends of the parallel molecules are staggered.If they were not, there would be a weak spot running right across the collagen fibre.What is the meaning of staggered ends here? Why would there be a weak spot running right across the collagen fibre if the ends are not staggered? Thank you ...
... , also known as β-aminopropionitrile (BAPN), is an organic compound with both amine and nitrile functional ... Beta-amino-propionitrile (BAPN) found in lathyrus odoratus (common garden sweet pea) is thought to be responsible for ... Aminopropionitrile is prepared by the reaction of ammonia with acrylonitrile. Kashin-Beck disease Lysyl oxidase Marfan syndrome ... "Aminopropionitrile - Compound Summary". PubChem Compound. USA: National Center for Biotechnology Information. 25 March 2005. ...
It is caused by a different toxin, beta-aminopropionitrile, which affects the linking of the subunits of collagen, a major ... It is also caused by the toxin beta-aminopropionitrile. Eating the grasspea with legumes having high concentrations of sulphur- ... employing the toxin beta-aminopropionitrile. The blood vessels are affected, as opposed to bone. Neurolathyrism is caused by ...
It is prepared by the amination of acrylonitrile followed by hydrogenation of the resulting aminopropionitrile. The potassium ...
... modulation of balance between proteoglycan and collagen network in vitro with beta-aminopropionitrile". Osteoarthritis and ...
They went on to explore how aminopropionitrile in sweet peas could be bound with polymers, and created CryoSkin. They were ...
It is caused by a different toxin (beta-aminopropionitrile) which affects the linking of collagen, a protein of connective ... The cause of this disease is attributed to beta-aminopropionitrile, which inhibits the copper-containing enzyme lysyl oxidase, ... It is a skeletal disorder, caused by the toxin beta-aminopropionitrile (BAPN), and characterized by hernias, aortic dissection ...
The most widely studied of these compounds is beta-aminopropionitrile (BAPN), which exerts its deleterious effect by an unknown ... beta-aminopropionitrile), which affects the linking of collagen, a protein of connective tissues. The condition results in ...
The main chemical responsible is β-Aminopropionitrile, which prevents collagen cross-linking, thus making the blood vessel, ...
A recent study has shown that β-Aminopropionitrile plus elastase application to abdominal aorta causes more severe aneurysm in ...
Seeds of the sweet pea contain beta-aminopropionitrile that prevents the cross-linking of collagen by inhibiting lysyl oxidase ...
... combinations QM01AX90 Pentosan polysulfate QM01AX91 Aminopropionitrile QM01AX92 Grapiprant QM01AX99 Combinations M01BA01 ...
... amino]propionitrile) and any salt thereof Mefenorex (d,l-N-(3-chloropropyl)-α-methylbenzeneethanamine) and any salt thereof ...
The molecular formula C3H6N2 (molar mass: 70.09 g/mol, exact mass: 70.0531 u) may refer to: Aminopropionitrile (BAPN) ...
... lysyl oxidase was inhibited either by nutritional copper-deficiency or by supplementation of diets with β-aminopropionitrile ( ...
Effect of topical beta-aminopropionitrile on connective tissue of granulomas. Indian Journal of Experimental Biology. 1988 Apr ...
Dive into the research topics of Inhibition of collagen aggregation in rat cartilage induced by beta amino propionitrile in ... Inhibition of collagen aggregation in rat cartilage induced by beta amino propionitrile in vitro. ...
Fenproporex (d,l-3-[(α-methylphenethyl)amino]propionitrile) and any salt thereof. 7. Mazindol (5-(p-chlorophenyl)-2,5-dihydro- ...
Kumar D, Trent MB, Boor PJ: Allylamine and beta-aminopropionitrile induced aortic medial necrosis: mechanisms of synergism. ...
Knockout of ALDH2 mitigated β-aminopropionitrile-induced TAD formation in animal studies. Ultrasound results showed that ALDH2 ... T cells and T helper cells were decreased in ALDH2 knockout mice treated with β-aminopropionitrile for 28 days. ALDH2 knockout ... Three-week-old male mice were administered freshly prepared β-aminopropionitrile solution dissolved in drinking water (1 g ... β-aminopropionitrile (BAPN) treatment led to AD formation by inhibiting lysyl oxidase activity [15]. Three-week-old male WT ...
... or beta-aminopropionitrile, which interferes with collagen crosslinking. We also focused on intervention after the initiation ...
For this function, we have examined the effect of -aminopropionitrile (BAPN), a particular and irreversible inhibitor of LOX ... In obese rats, the inhibition of LOX activity through -aminopropionitrile (BAPN, a particular inhibitor of LOX activity) ...
Collagen crosslinking was inhibited by treating embryos in E3 water with the lysyl oxidase inhibitor β-aminopropionitrile (30 ...
Aminopropionitrile - Preferred Concept UI. M0000960. Scope note. Reagent used as an intermediate in the manufacture of beta- ...
Beta-aminopropionitrile Active Synonym false false 92386010 Lathyrus poison Active Synonym false false ...
Piroxicam is a nonsteroidal anti-inflammatory drug (NSAID) of the oxicam class used to relieve the symptoms of painful inflammatory conditions like arthritis.[3][4] Piroxicam works by preventing the production of endogenous prostaglandins which are involved in the mediation of pain, stiffness, tenderness and swelling.[3] The medicine is available as capsules, tablets and (not in all countries) as a prescription-free gel 0.5%.[5] It is also available in a betadex formulation, which allows a more rapid absorption of piroxicam from the digestive tract.[3] Piroxicam is one of the few NSAIDs that can be given parenteral routes.[citation needed] It was patented in 1968 by Pfizer and approved for medical use in 1979.[6] It became generic in 1992,[7] and is marketed worldwide under many brandnames.[1] ...
Dive into the research topics of Effect of lysyl oxidase inhibition on angiotensin ii-induced arterial hypertension, remodeling, and stiffness. Together they form a unique fingerprint. ...
Indeed, LOX inhibition by ß-aminopropionitrile prevented the gelatin stiffness increase. CONCLUSIONS: These data indicate that ...
BAPN use Aminopropionitrile BAPNA use Benzoylarginine Nitroanilide Baptisia use Fabaceae Baptisia australis use Baptisia ...
β-aminopropionitrile (BAPN) is an irreversible inhibitor of lysyl oxidase that induces TAAD in animals. The objective of this ... and Mortality in a Mouse Model of Beta-Aminopropionitrile-Induced Aortic Disease. *Brittany O Aicher, ...
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Aminopropionitrile [D02.626.151] * Amygdalin [D02.626.175] * Anastrozole [D02.626.218] * Carbonyl Cyanide m-Chlorophenyl ...
It can cause aortic aneurism as it contains a chemical called Aminopropionitrile which inhibits the action of Lysil oxidase in ...
... ß-aminopropionitrile, an irreversible inhibitor of lysyl oxidases. (7) ...
... ß-aminopropionitrile, an irreversible inhibitor of lysyl oxidases. (31). Extracellular copper enzymes initiate the formation of ...
... to mice in which aortic dissection was induced using beta-aminopropionitrile (BAPN) and angiotensin II (Ang II) infusion (2 ...
Descritores em Ciências da Saúde
BAPN use Aminopropionitrile BAPNA use Benzoylarginine Nitroanilide Baptisia use Fabaceae Baptisia australis use Baptisia ...
BAPN use Aminopropionitrile BAPNA use Benzoylarginine Nitroanilide Baptisia use Fabaceae Baptisia australis use Baptisia ...
BAPN use Aminopropionitrile BAPNA use Benzoylarginine Nitroanilide Baptisia use Fabaceae Baptisia australis use Baptisia ...
BAPN use Aminopropionitrile BAPNA use Benzoylarginine Nitroanilide Baptisia use Fabaceae Baptisia australis use Baptisia ...
Aminopropionitrile. *Amygdalin. *Carbonyl Cyanide m-Chlorophenyl Hydrazone. *Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone ...
3-Aminopropionitrile. Phenylacetic Acid. Silver(II) Oxide. Fluorosulfonic Acid. Candoxatril. Fomocaine. Pyridoxamine ...
  • For this function, we have examined the effect of -aminopropionitrile (BAPN), a particular and irreversible inhibitor of LOX activity, inside a style of diet-induced weight problems. (exposed-skin-care.net)
  • In obese rats, the inhibition of LOX activity through -aminopropionitrile (BAPN, a particular inhibitor of LOX activity) decreases adipose cells fibrosis, partly corrects the adipocyte-size distribution design (moving it toward smaller sized sizes) and attenuates the upsurge in bodyweight and extra fat mass. (exposed-skin-care.net)
  • IMSEAR at SEARO: Effect of topical beta-aminopropionitrile on connective tissue of granulomas. (who.int)
  • For example, Riley et al have prevented fibrosis in animal models by administering either proline analogues, which interfere with proper folding of collagen, or beta-aminopropionitrile, which interferes with collagen crosslinking. (cdc.gov)
  • Because collagens and elastin are important components of the extracellular matrix, abnormalities in their modification can be expected to affect many tissues, as seen in lathyrism, a connective tissue disorder caused by the administration of ß-aminopropionitrile, an irreversible inhibitor of lysyl oxidases. (adhd-npf.com)
  • The most promising method seems to be the blocking of crosslinks formation among collagen molecules by β-aminopropionitrile, a competitive inhibitor of a crosslinking enzyme, lysyl oxidase. (nih.gov)
  • Because collagens and elastin are important components of the extracellular matrix, abnormalities in their modification can be expected to affect many tissues, as seen in lathyrism, a connective tissue disorder caused by the administration of ß-aminopropionitrile, an irreversible inhibitor of lysyl oxidases. (adhd-npf.com)
  • Establishment and effect evaluation of an aortic dissection model induced by different doses of β-aminopropionitrile in rats. (medscape.com)
  • The emission of 2-aminopropionitrile was modeled simultaneously with the emission of all molecules known in Sgr B2(N), which allowed us to properly take into account line blending and avoid misassignments. (u-strasbg.fr)
  • Rotational spectrum of a chiral amino acid precursor, 2-aminopropionitrile, and searches for it in Sagittarius B2 (N). (u-strasbg.fr)