GTP Cyclohydrolase: (GTP cyclohydrolase I) or GTP 7,8-8,9-dihydrolase (pyrophosphate-forming) (GTP cyclohydrolase II). An enzyme group that hydrolyzes the imidazole ring of GTP, releasing carbon-8 as formate. Two C-N bonds are hydrolyzed and the pentase unit is isomerized. This is the first step in the synthesis of folic acid from GTP. EC 3.5.4.16 (GTP cyclohydrolase I) and EC 3.5.4.25 (GTP cyclohydrolase II).Riboflavin: Nutritional factor found in milk, eggs, malted barley, liver, kidney, heart, and leafy vegetables. The richest natural source is yeast. It occurs in the free form only in the retina of the eye, in whey, and in urine; its principal forms in tissues and cells are as FLAVIN MONONUCLEOTIDE and FLAVIN-ADENINE DINUCLEOTIDE.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)AmidinesBiopterin: A natural product that has been considered as a growth factor for some insects.Pterins: Compounds based on 2-amino-4-hydroxypteridine.AminohydrolasesPteridines: Compounds based on pyrazino[2,3-d]pyrimidine which is a pyrimidine fused to a pyrazine, containing four NITROGEN atoms.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Methenyltetrahydrofolate Cyclohydrolase: An aminohydrolase that catalyzes the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate. In most higher eucaryotic organisms this enzyme also includes METHYLENETETRAHYDROFOLATE DEHYDROGENASE (NADP) and FORMATE-TETRAHYDROFOLATE LIGASE activities.Streptococcus pneumoniae: A gram-positive organism found in the upper respiratory tract, inflammatory exudates, and various body fluids of normal and/or diseased humans and, rarely, domestic animals.N-Acetylmuramoyl-L-alanine Amidase: An autolytic enzyme bound to the surface of bacterial cell walls. It catalyzes the hydrolysis of the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell wall glycopeptides, particularly peptidoglycan. EC 3.5.1.28.Pneumococcal Infections: Infections with bacteria of the species STREPTOCOCCUS PNEUMONIAE.Serotyping: Process of determining and distinguishing species of bacteria or viruses based on antigens they share.Bacterial Capsules: An envelope of loose gel surrounding a bacterial cell which is associated with the virulence of pathogenic bacteria. Some capsules have a well-defined border, whereas others form a slime layer that trails off into the medium. Most capsules consist of relatively simple polysaccharides but there are some bacteria whose capsules are made of polypeptides.Cell Wall: The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.Streptococcus: A genus of gram-positive, coccoid bacteria whose organisms occur in pairs or chains. No endospores are produced. Many species exist as commensals or parasites on man or animals with some being highly pathogenic. A few species are saprophytes and occur in the natural environment.Bacteriolysis: Rupture of bacterial cells due to mechanical force, chemical action, or the lytic growth of BACTERIOPHAGES.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Autolysis: The spontaneous disintegration of tissues or cells by the action of their own autogenous enzymes.Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Rabbits: The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.Adenosine Deaminase: An enzyme that catalyzes the hydrolysis of ADENOSINE to INOSINE with the elimination of AMMONIA.Nucleoside Deaminases: Catalyze the hydrolysis of nucleosides with the elimination of ammonia.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.Antibody Specificity: The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Peptides: Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.Epitopes: Sites on an antigen that interact with specific antibodies.

MOT1 can activate basal transcription in vitro by regulating the distribution of TATA binding protein between promoter and nonpromoter sites. (1/447)

MOT1 is an ATPase which can dissociate TATA binding protein (TBP)-DNA complexes in a reaction requiring ATP hydrolysis. Consistent with this observation, MOT1 can repress basal transcription in vitro. Paradoxically, however, some genes, such as HIS4, appear to require MOT1 as an activator of transcription in vivo. To further investigate the function of MOT1 in basal transcription, we performed in vitro transcription reactions using yeast nuclear extracts depleted of MOT1. Quantitation of MOT1 revealed that it is an abundant protein, with nuclear extracts from wild-type cells containing a molar excess of MOT1 over TBP. Surprisingly, MOT1 can weakly activate basal transcription in vitro. This activation by MOT1 is detectable with amounts of MOT1 that are approximately stoichiometric to TBP. With amounts of MOT1 similar to those present in wild-type nuclear extracts, MOT1 behaves as a weak repressor of basal transcription. These results suggest that MOT1 might activate transcription via an indirect mechanism in which limiting TBP can be liberated from nonpromoter sites for use at promoters. In support of this idea, excess nonpromoter DNA sequesters TBP and represses transcription, but this effect can be reversed by addition of MOT1. These results help to reconcile previous in vitro and in vivo results and expand the repertoire of transcriptional control strategies to include factor-assisted redistribution of TBP between promoter and nonpromoter sites.  (+info)

A methenyl tetrahydromethanopterin cyclohydrolase and a methenyl tetrahydrofolate cyclohydrolase in Methylobacterium extorquens AM1. (2/447)

Recently it was found that Methylobacterium extorquens AM1 contains both tetrahydromethanopterin (H4MPT) and tetrahydrofolate (H4F) as carriers of C1 units. In this paper we report that the aerobic methylotroph contains a methenyl H4MPT cyclohydrolase (0.9 U x mg-1 cell extract protein) and a methenyl H4F cyclohydrolase (0.23 U x mg-1). Both enzymes, which were specific for their substrates, were purified and characterized and the encoding genes identified via the N-terminal amino acid sequence. The purified methenyl H4MPT cyclohydrolase with a specific activity of 630 U x mg-1 (Vmax = 1500 U x mg-1; Km = 30 microm) was found to be composed of two identical subunits of molecular mass 33 kDa. Its sequence was approximately 40% identical to that of methenyl H4MPT cyclohydrolases from methanogenic archaea. The methenyl H4F cyclohydrolase with a specific activity of 100 U x mg-1 (Vmax = 330 U x mg-1; Km = 80 microm) was found to be composed of two identical subunits of molecular mass 22 kDa. Its sequence was not similar to that of methenyl H4MPT cyclohydrolases or to that of other methenyl H4F cyclohydrolases. Based on the specific activities in cell extract and from the growth properties of insertion mutants it is suggested that the methenyl H4MPT cyclohydrolase might have a catabolic, and the methenyl-H4F cyclohydrolase an anabolic function in the C1-unit metabolism of M. extorquens AM1.  (+info)

Maximal stimulation of meiotic recombination by a yeast transcription factor requires the transcription activation domain and a DNA-binding domain. (3/447)

The DNA sequences located upstream of the yeast HIS4 represent a very strong meiotic recombination hotspot. Although the activity of this hotspot requires the transcription activator Rap1p, the level of HIS4 transcription is not directly related to the level of recombination. We find that the recombination-stimulating activity of Rap1p requires the transcription activation domain of the protein. We show that a hybrid protein with the Gal4p DNA-binding domain and the Rap1p activation domain can stimulate recombination in a strain in which Gal4p-binding sites are inserted upstream of HIS4. In addition, we find recombination hotspot activity associated with the Gal4p DNA-binding sites that is independent of known transcription factors. We suggest that yeast cells have two types of recombination hotspots, alpha (transcription factor dependent) and beta (transcription factor independent).  (+info)

The crystal structure of a bacterial, bifunctional 5,10 methylene-tetrahydrofolate dehydrogenase/cyclohydrolase. (4/447)

The structure of a bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/cyclohydrolase from Escherichia coli has been determined at 2.5 A resolution in the absence of bound substrates and compared to the NADP-bound structure of the homologous enzyme domains from a trifunctional human synthetase enzyme. Superposition of these structures allows the identification of a highly conserved cluster of basic residues that are appropriately positioned to serve as a binding site for the poly-gamma-glutamyl tail of the tetrahydrofolate substrate. Modeling studies and molecular dynamic simulations of bound methylene-tetrahydrofolate and NADP shows that this binding site would allow interaction of the nicotinamide and pterin rings in the dehydrogenase active site. Comparison of these enzymes also indicates differences between their active sites that might allow the development of inhibitors specific to the bacterial target.  (+info)

Chromatin opening and transactivator potentiation by RAP1 in Saccharomyces cerevisiae. (5/447)

Transcriptional activators function in vivo via binding sites that may be packaged into chromatin. Here we show that whereas the transcriptional activator GAL4 is strongly able to perturb chromatin structure via a nucleosomal binding site in yeast, GCN4 does so poorly. Correspondingly, GCN4 requires assistance from an accessory protein, RAP1, for activation of the HIS4 promoter, whereas GAL4 does not. The requirement for RAP1 for GCN4-mediated HIS4 activation is dictated by the DNA-binding domain of GCN4 and not the activation domain, suggesting that RAP1 assists GCN4 in gaining access to its binding site. Consistent with this, overexpression of GCN4 partially alleviates the requirement for RAP1, whereas HIS4 activation via a weak GAL4 binding site requires RAP1. RAP1 is extremely effective at interfering with positioning of a nucleosome containing its binding site, consistent with a role in opening chromatin at the HIS4 promoter. Furthermore, increasing the spacing between binding sites for RAP1 and GCN4 by 5 or 10 bp does not impair HIS4 activation, indicating that cooperative protein-protein interactions are not involved in transcriptional facilitation by RAP1. We conclude that an important role of RAP1 is to assist activator binding by opening chromatin.  (+info)

A set of independent selectable markers for transfection of the human malaria parasite Plasmodium falciparum. (6/447)

Genomic information is rapidly accumulating for the human malaria pathogen, Plasmodium falciparum. Our ability to perform genetic manipulations to understand Plasmodium gene function is limited. Dihydrofolate reductase is the only selectable marker presently available for transfection of P. falciparum. Additional markers are needed for complementation and for expression of mutated forms of essential genes. We tested parasite sensitivity to different drugs for which selectable markers are available. Two of these drugs that were very effective as antiplasmodial inhibitors in culture, blasticidin and geneticin (G418), were selected for further study. The genes BSD, encoding blasticidin S deaminase of Aspergillus terreus, and NEO, encoding neomycin phosphotransferase II from transposon Tn 5, were expressed under the histidine-rich protein III (HRPIII) gene promoter and tested for their ability to confer resistance to blasticidin or G418, respectively. After transfection, blasticidin and G418-resistant parasites tested positive for plasmid replication and BSD or NEO expression. Cross-resistance assays indicate that these markers are independent. The plasmid copy number and the enzymatic activity depended directly on the concentration of the drug used for selection. These markers set the stage for new methods of functional analysis of the P. falciparum genome.  (+info)

Efficient expression, purification and crystallisation of two hyperthermostable enzymes of histidine biosynthesis. (7/447)

Enzymes from hyperthermophiles can be efficiently purified after expression in mesophilic hosts and are well-suited for crystallisation attempts. Two enzymes of histidine biosynthesis from Thermotoga maritima, N'-((5'-phosphoribosyl)-formimino)-5-aminoimidazol-4-carb oxamid ribonucleotide isomerase and the cyclase moiety of imidazoleglycerol phosphate synthase, were overexpressed in Escherichia coli, both in their native and seleno-methionine-labelled forms, purified by heat precipitation of host proteins and crystallised. N'-((5'-phosphoribosyl)-formimino)-5-aminoimidazol-4-carb oxamid ribonucleotide isomerase crystallised in four different forms, all suitable for X-ray structure solution, and the cyclase moiety of imidazoleglycerol phosphate synthase yielded one crystal form that diffracted to atomic resolution. The obtained crystals will enable the determination of the first three-dimensional structures of enzymes from the histidine biosynthetic pathway.  (+info)

Distribution of tetrahydromethanopterin-dependent enzymes in methylotrophic bacteria and phylogeny of methenyl tetrahydromethanopterin cyclohydrolases. (8/447)

The methylotrophic proteobacterium Methylobacterium extorquens AM1 possesses tetrahydromethanopterin (H(4)MPT)-dependent enzymes, which are otherwise specific to methanogenic and sulfate-reducing archaea and which have been suggested to be involved in formaldehyde oxidation to CO(2) in M. extorquens AM1. The distribution of H(4)MPT-dependent enzyme activities in cell extracts of methylotrophic bacteria from 13 different genera are reported. H(4)MPT-dependent activities were detected in all of the methylotrophic and methanotrophic proteobacteria tested that assimilate formaldehyde by the serine or ribulose monophosphate pathway. H(4)MPT-dependent activities were also found in autotrophic Xanthobacter strains. However, no H(4)MPT-dependent enzyme activities could be detected in other autotrophic alpha-proteobacteria or in gram-positive methylotrophic bacteria. Genes encoding methenyl H(4)MPT cyclohydrolase (mch genes) were cloned and sequenced from several proteobacteria. Bacterial and archaeal Mch sequences have roughly 35% amino acid identity and form distinct groups in phylogenetic analysis.  (+info)

*Aminohydrolase

An aminohydrolase is a hydrolase enzyme which acts upon an amino group. Aminohydrolases are classified under EC number EC 3.5.4 ... Aminohydrolases at the US National Library of Medicine Medical Subject Headings (MeSH) Molecular and Cellular Biology portal. ...

*Aminopyrimidine aminohydrolase

... (EC 3.5.99.2, thiaminase, thiaminase II, tenA (gene)) is an enzyme with systematic name 4-amino- ... Aminopyrimidine aminohydrolase at the US National Library of Medicine Medical Subject Headings (MeSH) Molecular and Cellular ... 5-aminomethyl-2-methylpyrimidine aminohydrolase. This enzyme catalyses the following chemical reaction (1) 4-amino-5- ...

*5-nitroanthranilic acid aminohydrolase

... (EC 3.5.99.8, naaA (gene), 5NAA deaminase) is an enzyme with systematic name 5- ... 5-nitroanthranilic acid aminohydrolase at the US National Library of Medicine Medical Subject Headings (MeSH) Molecular and ...

*Nitrilase

... enzymes (nitrile aminohydrolase; EC 3.5.5.1) catalyse the hydrolysis of nitriles to carboxylic acids and ammonia, ...

*ADP deaminase

The systematic name of this enzyme class is ADP aminohydrolase. Other names in common use include adenosine diphosphate ...

*DCTP deaminase

The systematic name of this enzyme class is dCTP aminohydrolase. Other names in common use include deoxycytidine triphosphate ...

*ATP deaminase

The systematic name of this enzyme class is ATP aminohydrolase. This enzyme is also called adenosine triphosphate deaminase. ...

*Thiocyanate hydrolase

The systematic name of this enzyme class is thiocyanate aminohydrolase. As of late 2007, 4 structures have been solved for this ...

*Sepiapterin deaminase

The systematic name of this enzyme class is sepiapterin aminohydrolase. Tsusue M (April 1971). "Studies on sepiapterin ...

*Adenine deaminase

The systematic name of this enzyme class is adenine aminohydrolase. Other names in common use include adenase, adenine aminase ...

*Arylacetonitrilase

The systematic name of this enzyme class is arylacetonitrile aminohydrolase. This enzyme participates in cyanoamino acid ...

*Cytosine deaminase

The systematic name of this enzyme class is cytosine aminohydrolase. This enzyme is also called isocytosine deaminase. This ...

*Guanosine deaminase

The systematic name of this enzyme class is guanosine aminohydrolase. This enzyme is also called guanosine aminase. Isihida Y, ...

*Ricinine nitrilase

The systematic name of this enzyme class is ricinine aminohydrolase. This enzyme participates in nitrogen metabolism. ROBINSON ...

*Aminoimidazolase

The systematic name of this enzyme class is 4-aminoimidazole aminohydrolase. This enzyme is also called 4-aminoimidazole ...

*Blasticidin-S deaminase

The systematic name of this enzyme class is blasticidin-S aminohydrolase. As of late 2007, two structures have been solved for ...

*Trypanothione synthase

The N-terminal domain is a cysteine, histidine-dependent aminohydrolase amidase. Structurally the synthetase and amidase ...

*2-aminomuconate deaminase

The systematic name of this enzyme class is 2-aminomuconate aminohydrolase. This enzyme participates in tryptophan metabolism. ...

*DCTP deaminase (dUMP-forming)

The systematic name of this enzyme class is dCTP aminohydrolase (dUMP-forming). This enzyme participates in pyrimidine ...

*Deoxycytidine deaminase

The systematic name of this enzyme class is cytidine/2'-deoxycytidine aminohydrolase. This enzyme participates in pyrimidine ...

*Pterin deaminase

The systematic name of this enzyme class is 2-amino-4-hydroxypteridine aminohydrolase. This enzyme is also called acrasinase. ...

*Cyanoalanine nitrilase

The systematic name of this enzyme class is 3-cyano-L-alanine aminohydrolase. This enzyme is also called beta-cyanoalanine ...

*S-adenosylhomocysteine deaminase

The systematic name of this enzyme class is S-adenosyl-L-homocysteine aminohydrolase. This enzyme is also called ...

*1-aminocyclopropane-1-carboxylate deaminase

The systematic name of this enzyme class is 1-aminocyclopropane-1-carboxylate aminohydrolase (isomerizing). This enzyme is also ...

*Methylenediurea deaminase

... (EC 3.5.3.21, methylenediurease) is an enzyme with systematic name methylenediurea aminohydrolase. ...
Burkholderia multivorans strain LMG17588 histidinol-phosphate aminotransferase (hisC) gene, partial cds; imidazole glycerol phosphate dehydratase (hisB), multiple antibiotic resistance-related protein (marC), imidazole glycerol phosphate synthase glutamine amidotransferase subunit (hisH), phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (hisA), imidazole glycerol phosphate synthase subunit (hisF), phosphoribosyl-AMP cyclohydrolase (hisI), and phosphoribosyl-ATP pyrophosphohydrolase (hisE) genes, complete cds; and membrane protein gene, partial ...
Burkholderia cenocepacia strain LMG19240 histidinol-phosphate aminotransferase (hisC) gene, partial cds; imidazole glycerol phosphate dehydratase (hisB), multiple antibiotic resistance-related protein (marC), imidazole glycerol phosphate synthase glutamine amidotransferase subunit (hisH), phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (hisA), imidazole glycerol phosphate synthase subunit (hisF), phosphoribosyl-AMP cyclohydrolase (hisI), and phosphoribosyl-ATP pyrophosphohydrolase (hisE) genes, complete cds; and membrane protein gene, partial ...
Burkholderia multivorans strain LMG17588 histidinol-phosphate aminotransferase (hisC) gene, partial cds; imidazole glycerol phosphate dehydratase (hisB), multiple antibiotic resistance-related protein (marC), imidazole glycerol phosphate synthase glutamine amidotransferase subunit (hisH), phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (hisA), imidazole glycerol phosphate synthase subunit (hisF), phosphoribosyl-AMP cyclohydrolase (hisI), and phosphoribosyl-ATP pyrophosphohydrolase (hisE) genes, complete cds; and membrane protein gene, partial ...
IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The glutamine amidotransferase domain provides the ammonia necessary to the cyclase domain to produce IGP and AICAR from PRFAR.
2A0N: Crystal structure of Imidazole glycerol phosphate synthase subunit hisF (EC 4.1.3.-) (tm1036) from Thermotoga maritima at 1.64 A resolution
The TN1036 gene from Thermotoga maritima encodes the cyclase subunit of imidazole-glycerol-3-phosphate synthase HisF (HisF-cyclase). This family belong to the common phosphate binding site TIM barrel family PF00977. Imidazole glycerol phosphate synthase catalyzes the fifth step of histidine biosynthesis, the formation of the imidazole ring. The enzyme converts N1-(5-phosphoribulosyl)-formimino-5-aminoimidazole-4-carboxamide ribonucleotide (PRFAR) to imidazole glycerol phosphate (ImGP) and 5-(5-aminoimidazole-4-carboxamide) ribonucleotide (AICAR). This conversion involves two tightly coupled reactions in distinct active sites of HisF. The two catalytic domains can be fused, like in fungi and plants, or preformed within a heterodimer (HisH-glutaminase and HisF-cyclase), like in bacteria. ...
1K9V: Structural evidence for ammonia tunneling across the (beta alpha)(8) barrel of the imidazole glycerol phosphate synthase bienzyme complex.
Imidazole glycerol phosphate synthase subunit HisF; IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit (266 aa ...
We have suggested an alignment of three cyanide degrading nitrilases to the crystallographically determined structures of Nit and DCase which was primarily created using GenTHREADER.. We have also aligned the structures of Nit and DCase using ALIGN. (see superpose.pdb). From this we have concluded that insertions and deletions in the cyanide degrading enzymes occur in externally located loops and that there are two major insertions, one major deletion and a substantial C-terminal extension.. An important difference between our enzymes, and indeed the majority of nitrilases, and the solved structures of superfamily members is the formation of spiral oligomers comprising specific numbers of subunits - but different numbers of subunits (ranging from 10-18) in different enzymes. We have postulated that the interaction that leads to the formation of the oligomer is due to an 15 amino acid insertion found in the loop between the beta strands labelled NS9 and NS10 in Nit. This, we postulate, leads to ...
Dear colleagues, can anyone direct me to a commercial source of imidazole glycerol or imidazole acetol? Please refrain from suggesting Sigma, Aldrich or Fluka Many thanks, Dudley Page ...
This gene encodes a member of the nitrilase protein family with homology to bacterial and plant nitrilases, enzymes that cleave nitriles and organic amides to the corresponding carboxylic acids plus ammonia. Multiple transcript variants encoding different isoforms have been found for this gene ...
This clade of sequences is highly similar to the HisF protein, but generally represents the second HisF homolog in the genome where the other is an authentic HisF observed in the context of a complete histidine biosynthesis operon. The similarity between these WbuZ sequences and true HisFs is such that often the closest match by BLAST of a WbuZ is a HisF. Only by making a multiple sequence alignment is the homology relationship among the WbuZ sequences made apparent. WbuZ genes are invariably observed in the presence of a homolog of the HisH protein (designated WbuY) and a proposed N-acetyl sugar amidotransferase designated in WbuX in E. coli [1], IfnA in P. aeriginosa [2] and PseA in C. jejuni [3]. Similarly, this trio of genes is invariably found in the context of saccharide biosynthesis loci. It has been shown that the WbuYZ homologs are not essential components of the activity expressed by WbuX, leading to the proposal that these to proteins provide ammonium ions to the amidotransferase when ...
Our metagenomic library, bioinformatics software and research grade sample service were used for the rapid identification of improved nitrilase enzymes.
Plays a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells. Has apparently no omega-amidase activity such as NIT2 (By similarity).
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Kupryjanowicz M., Nalepka D., Pidek I.A., Walanus A., Balwierz Z., Bi ka K., Fi oc M., Granoszewski W., Ko aczek P., Majecka A., Malkiewicz M., Nita M., Nory kiewicz B., Winter H. 2015. Eemska historia ro linno ci Polski w oparciu o mapy izopolowe. [W:] VII Konferencja Paleobotaniki Czwartorz du Dynamika zmian ro linno ci Ni u Polskiego w dobie p noglacjalnych zmian klimatu i narastania antropopresji w holocenie , d , 10 12 czerwca 2015: 34 36 ...
A. Visan, M. Miroiu, C. Nita, R. Cristescu, G. Socol, N. Stefan, G. Dorcioman, N. Serban, M. Socol, I. Zgura, O.L. Rasoga, C. Breazu, L. Sima, C. R. Luculescu, A. Stanculescu, I.N. Mihailescu ...
Shop Indole-3-glycerol phosphate synthase ELISA Kit, Recombinant Protein and Indole-3-glycerol phosphate synthase Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Indole-3-glycerol phosphate synthase (IGPS) catalyzes the irreversible ring closure of 1-(ocarboxyphenylamino)- 1-deoxyribulose 5- phosphate (CdRP) into indole-3-glycerol phosphate (IGP) during the fourth step in tryptophan biosynthesis. Analysis of the crystal structure of IGPS from Mycobacterium tuberculosis (MtIGPS) hinted the importance of Lys119 for binding or catalysis. Lys110 from sulfolobus sulfataricus IGPS (ssIGPS) that aligns with Lys119 from MtIGPS, was proposed to be a general acid for the proton transfer that initiates the ring closure and decarboxylation of CdRP. To study the importance of Lys119 in the chemical mechanism for MtIGPS, an amino acid change was made at this position to study any changes in catalytic function or substrate binding affinity. Kinetic assays were performed and interesting, a mutation to alanine had a dramatic effect on the activity of MtIGPS. Our studies highlight the importance of Lys119 in the MtIGPS-catalyzed chemical mechanism.
Guanine deaminase Proteins available through Novus Biologicals. Browse our Guanine deaminase Protein catalog backed by our Guarantee+.
Enzymes involved in tetrahydrofolate metabolism are of particular pharmaceutical interest, as their function is crucial for amino acid and DNA biosynthesis. The crystal structure of the human cytosolic methylenetetrahydrofolate dehydrogenase/cyclohydrolase (DC301) domain of a trifunctional enzyme has been determined previously with a bound NADP cofactor. While the substrate binding site was identified to be localized in a deep and rather hydrophobic cleft at the interface between two protein domains, the unambiguous assignment of catalytic residues was not possible. We succeeded in determining the crystal structures of three ternary DC301/NADP/inhibitor complexes. Investigation of these structures followed by site-directed mutagenesis studies allowed identification of the amino acids involved in catalysis by both enzyme activities. The inhibitors bind close to Lys56 and Tyr52, residues of a strictly conserved motif for active sites in dehydrogenases. While Lys56 is in a good position for ...
Nit Protein; One Of Two Proteins In S. Cerevisiae With Similarity To The Nit Domain Of NitFhit From Fly And Worm And To The Mouse And Human Nit Protein Which Interacts With The Fhit Tumor Suppressor; Nitrilase Superfamily Member
Dichotomous Effects of Isometric Secondary Amines Containing an Aromatic Nitrile and a Nitro Group on Human Aortic Smooth Muscle Cells: Syntheses, Nitrosation and Cell Culture Studies ...
Dichotomous Effects of Isometric Secondary Amines Containing an Aromatic Nitrile and a Nitro Group on Human Aortic Smooth Muscle Cells: Syntheses, Nitrosation and Cell Culture Studies ...
The following sections contain reference sequences that belong to a specific genome build. Explain. This section includes genomic Reference Sequences (RefSeqs) from all assemblies on which this gene is annotated, such as RefSeqs for chromosomes and scaffolds (contigs) from both reference and alternate assemblies. Model RNAs and proteins are also reported here.. ...
GenBank) Tryptophan biosynthesis protein trpCF [Indole-3-glycerol phosphate synthase, N-terminal (IGPS); N-(5-phosphoribosyl)anthranilate isomerase, C-terminal (PRAI ...
Nitrilase of Nocardia globerula NHB-2 was induced by short-chain aliphatic nitriles (valeronitrile , isobutyronitrile , butyronitrile , propionitrile) and exhibited activity towards aromatic nitriles (benzonitrile , 3-cyanopyridine , 4-cyanopyridine , m-tolunitrile , p-tolunitrile). Hyperinduction of nitrilase (6.67 U mg (DCW) (-1) , 18.7 U mL(-1)) was achieved in short incubation time (30 h, 30°C) through multiple feeding of isobutyronitrile in the growth medium. The nitrilase of this organism exhibits ...
Berezov, T T., "Activity of omega-amidase in human and animal malignant neoplasms. (russ.)" (1966). Subject Strain Bibliography 1966. 266 ...
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Plasmid lentiCas9-Blast from Dr. Feng Zhangs lab contains the inserts Cas9 and Blasticidin resistance and is published in Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. This plasmid is available through Addgene.
Order Recombinant Prochlorococcus marinus subsp pastoris Indole-3-glycerol phosphate synthase trpC 01015965649 at Gentaur Prochlorococcus marinus subsp. pastoris Indole-3-glycerol phosphate synthase (trpC)
In eukaryotes, folate-dependent one-carbon (1-C) metabolism is composed of two parallel pathways compartmentalized to either the cytoplasm or mitochondria. In each, 1-C units, carried on tetrahydrofolate (THF), are interconverted by four catalytic activities. Serine hydroxymethyltransferase transfers the 3-carbon of serine to THF forming 5,10-methylene-THF which is oxidized in 3 successive steps to formate via the intermediates, 5,10-methenyl-THF and 10-formyl-THF. Because of the redox potential in each compartment, 1-C flux is thought by most authors to be from formate to serine in the cytosol and in the opposite direction in mitochondria. Transport of serine, glycine and formate across the mitochondrial membranes creates a 1-C cycle. All eukaryotes characterized to date contain a cytoplasmic trifunctional C1-THF synthase possessing 5,10-methylene-THF dehydrogenase, 5,10-methenyl-THF cyclohydrolase and 10-formyl-THF synthetase activities which interconvert the catalytic intermediates between ...
ID TRPC_SYNR3 Reviewed; 294 AA. AC A5GRL0; DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot. DT 12-JUN-2007, sequence version 1. DT 11-DEC-2019, entry version 64. DE RecName: Full=Indole-3-glycerol phosphate synthase {ECO:0000255,HAMAP-Rule:MF_00134}; DE Short=IGPS {ECO:0000255,HAMAP-Rule:MF_00134}; DE EC=4.1.1.48 {ECO:0000255,HAMAP-Rule:MF_00134}; GN Name=trpC {ECO:0000255,HAMAP-Rule:MF_00134}; GN OrderedLocusNames=SynRCC307_0616; OS Synechococcus sp. (strain RCC307). OC Bacteria; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus; OC unclassified Synechococcus. OX NCBI_TaxID=316278; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=RCC307; RG Genoscope; RL Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+) CC = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O; CC Xref=Rhea:RHEA:23476, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:16526, ...
Understanding how the flexibility inherent in protein structures is related to their amazing catalytic power is a timely question that has applications in drug development and protein design. My review in 2008 in Nature Chemical Biology discusses this concept extensively. I and my students are working on understanding this important problem in two model systems: Dihydrofolate reductase from Geobacillus stearothermophilus (DHFR) and Indole-3-glycerol phosphate synthase from Sulfolobus solfataricus (IGPS). Working on one of these projects, a student will be trained in mutagenesis, protein expression and purification, attachment of probes to proteins, enzyme kinetics, ligand binding measurements and computational modeling of enzyme kinetics ...
Live attenuated vaccines are superior to the killed or subunit vaccines. We designed a Salmonella Typhimurium strain by deleting folD gene (encoding methylenetetrahydrofolate dehydrogenase-cyclohydrolase) in the presence of a heterologous fhs gene (encoding formyltetrahydrofolate synthetase) and tested its vaccine potential under stringent conditions of lethal and sub-lethal challenges with virule ...
To sustain their proliferation, cancer cells become dependent on one-carbon metabolism to support purine and thymidylate synthesis. Indeed, one of the most highly upregulated enzymes during neoplastic transformation is MTHFD2, a mitochondrial methylenetetrahydrofolate dehydrogenase and cyclohydrolase involved in one-carbon metabolism. Because MTHFD2 is expressed normally only during embryonic development, it offers a disease-selective therapeutic target for eradicating cancer cells while sparing healthy cells. Here we report the synthesis and preclinical characterization of the first inhibitor of human MTHFD2. We also disclose the first crystal structure of MTHFD2 in complex with a substrate-based inhibitor and the enzyme cofactors NAD+ and inorganic phosphate. Our work provides a rationale for continued development of a structural framework for the generation of potent and selective MTHFD2 inhibitors for cancer treatment. Cancer Res; 77(4); 937-48. ©2017 AACR. ...
Aryl nitriles are important structural motifs found in pharmaceuticals, agrochemicals, and natural products.1 Furthermore, aromatic nitriles are versatile synthetic precursors and useful intermediates because they can be easily transformed into a diverse class of functionalities, such as amides, esters, primary amines, aldehydes, and carboxylic acids.2 Consequently, the development of efficient methods for the synthesis of aryl nitriles has drawn much attention from the organic synthetic community.3 The Sandmeyer and Rosenmund-von Braun reactions are two typical methods for the preparation of aromatic nitriles.4 The transition metal-catalyzed cyanations of aryl halides, aryl boronic acids and arenes with various cyanating agents have emerged as an alternative route to aryl nitriles.5 However, these protocols suffer from drawbacks, such as the use of an expensive transition-metal catalyst or stoichiometric amounts of mostly toxic metal cyanides and harsh reaction conditions.6 Recently, the search ...
SEQUESTRATION OF FORMALDEHYDE TO STABILIZE NITRILASE SPECIFIC ACTIVITY WHEN CONVERTING GLYCOLONITRILE TO GLYCOLIC ACID - diagram, schematic, and image 63 ...
SEQUESTRATION OF FORMALDEHYDE TO STABILIZE NITRILASE SPECIFIC ACTIVITY WHEN CONVERTING GLYCOLONITRILE TO GLYCOLIC ACID - diagram, schematic, and image 82 ...
Visit Healthgrades for information on Dr. Nita Modi, MD Find Phone & Address information, medical practice history, affiliated hospitals and more.
GT:ID BAC68253.1 GT:GENE folD2 GT:PRODUCT putative methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase GT:DATABASE GIB00135CH01 GT:ORG save0 GB:ACCESSION GIB00135CH01 GB:LOCATION complement(687276..688139) GB:FROM 687276 GB:TO 688139 GB:DIRECTION - GB:GENE folD2 GB:PRODUCT putative methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase GB:NOTE PF02882: Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain GB:PROTEIN_ID BAC68253.1 LENGTH 287 SQ:AASEQ MSATQTAQLMDGTGHARRIVEEAAAKAAEISQRTGTAPCLATVLVGDDPASVTYVRMKRARCAKAGIRSRHIALPATTTTAELIDSLSGLSGDPEVHGILLQHPCGPHIDERAAFEAIAPAKDVDGVTMHSFAAMSFGLPGFVSCTPGGIMRLLEAYDVDLAGKHAVVVGRSAILGKPVGMLLLAKDATVTYCHSRTADLSAMVREADVVVAAVGRPRLIRGEDIKPGAVVIDAGYNPGNVGDVDFDAVLTRARLITPVPGGVGPMTIAVLLEQTVDAAANQLGVQQ GT:EXON 1,1-287:0, SW:ID FOLD1_STRAW SW:DE RecName: Full=Bifunctional protein folD 1;Includes: RecName: Full=Methylenetetrahydrofolate dehydrogenase; EC=1.5.1.5;Includes: RecName: ...
Formate--tetrahydrofolate ligase (EC 6.3.4.3) (formyltetrahydrofolate synthetase) (FTHFS) is one of the enzymes participating in the transfer of one-carbon units, an essential element of various biosynthetic pathways. In many of these processes the transfers of one-carbon units are mediated by the coenzyme tetrahydrofolate (THF). Various reactions generate one-carbon derivatives of THF which can be interconverted between different oxidation states by FTHFS, methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5) and methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9). In eukaryotes the FTHFS activity is expressed by a multifunctional enzyme, C-1-tetrahydrofolate synthase (C1-THF synthase), which also catalyzes the dehydrogenase and cyclohydrolase activities. Two forms of C1-THF synthases are known [1], one is located in the mitochondrial matrix, while the second one is cytoplasmic. In both forms the FTHFS domain consist of about 600 amino acid residues and is located in the C-terminal section of ...
Enzymes that participate in the transfer of one-carbon units are involved in various biosynthetic pathways. In many of these processes the transfers of one-carbon units are mediated by the coenzyme tetrahydrofolate (THF). Various reactions generate one-carbon derivatives of THF which can be interconverted between different oxidation states by formyltetrahydrofolate synthetase (EC 6.3.4.3), methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5 or EC 1.5.1.15) and methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9 ...
Mono- and Stereopictres of 5.0 Angstrom coordination sphere of Zinc atom in PDB 1tiy: X-Ray Structure of Guanine Deaminase From Bacillus Subtilis Northeast Structural Genomics Consortium Target SR160
The PND association is a disease organization representing children and families who are affected by a pediatric neurotransmitter disease.. As a rare disease advocacy organization our mission is to represent and be a voice for all children affected by dopamine related PNDs. Our goals are to help children and families who are affected by PNDs, support the identification of new PNDs, find better treatments and ultimately a cure for those diseases that are already known. The PND Association was founded in 1998 and is a non-profit, voluntary organization.. All information contained on the PND Association website is intended for informational and educational purposes. The information is not intended to be a replacement or substitute for professional medical treatment or for professional medical advice relative to a specific medical question or condition. ...
MetabolismAmino acid biosynthesisHistidine familyimidazole glycerol phosphate synthase, glutamine amidotransferase subunit (TIGR01855; EC 2.4.2.-; HMM-score: 13.6) ...
Results: To elucidate the role of acetylpolyamines and their enzymatic deacetylation in more detail, all three putative acetylpolyamine amidohydrolases (APAHs) from P. aeruginosa have been expressed in enzymatic active form. The APAHs PA0321 and PA1409 are shown to be true polyamine deacetylases, whereas PA3774 is not able to deacetylate acetylated polyamines. Every APAH can hydrolyze trifluoroacetylated lysine-derivatives, but only PA1409 and much more efficiently PA3774 can also process the plain acetylated lysine substrate. P. aeruginosa is able to utilize acetylcad ... mehraverine and acetylputrescine as a carbon source under glucose starvation. If either the PA0321 or the PA1409 but not the PA3774 gene is disrupted, the growth of P. aeruginosa is reduced and delayed. In addition, we were able to show that the APAH inhibitors SAHA and SATFMK induce biofilm formation in both PA14 and PAO1 wildtype strains ...
anthranilate synthase / indole-3-glycerol phosphate synthase / phosphoribosylanthranilate isomerase [EC:4.1.3.27 4.1.1.48 5.3.1.24 ...
High-throughput methods for structural genomics have produced an increasing number of protein structures to be solved by X-ray crystallography. The abundance of protein structure information in the Protein Data Bank (PDB) has increased the need and desire for structure-based function prediction [1] and has contributed to structure-based drug design [2]. However, two problems remain regarding the prediction of enzyme function. First, proteins within a superfamily, which are usually expected to share the same catalytic properties, can catalyze different reactions. There are reports that enzymes with 98% sequence identity, such as melamine deaminase and atrazine chlorohydrolase, may catalyze different reactions [3]. Second, two enzymes belonging to different superfamilies or fold classes can catalyze almost identical reactions [4].. The function of a protein can be affected by a small number of residues in a localized region of its three-dimensional structure [5]. Moreover, the specific arrangement ...
Read independent reviews on Negative control lentivirus / lentiviral particles (non-translated spacer insert) with Blasticidin marker from AMS Biotechnology (Archived Products) on SelectScience
Histidine biosynthesis bifunctional protein HisIE; Protein involved in phosphoribosyl-AMP cyclohydrolase activity, phosphoribosyl-ATP diphosphatase activity and histidine biosynthetic process; In the N-terminal section; belongs to the PRA-CH family (213 aa ...
Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin),Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin) 04/05/12]] [http://openwetware.org/images/2/27/Cell_Media_with_Gen_Selection_Agents_4-5-2012.docx .],br ...
Whats the evidence for 5 portions of fruit and vegetables per day? And do 5 apples add up to the same health benefit as mixed greens...?Chris - Nita, keen on exercise?Nita - Sure, but keen on the other side of the energy balance equationtoo, which is the diet,which I think Dan just started talking about there.Chris - So, youre an epidemiologist.Nita - Yes.
The IGP yesterday instructed police officials not to stop Cattle-Laden Trucks carrying sacrificial animals on the highways unless there is a specific allegation.
[ATTACH] [ATTACH] Morning Folks, Attached is a screen grab of the MTHFD1 from my 23 & Me Browse Raw Data results. The purpose of this post is...
Buy our Recombinant Human NIT1 protein. Ab159001 is a protein fragment produced in Wheat germ and has been validated in WB, ELISA. Abcam provides free…
Shop Deoxycytidylate deaminase ELISA Kit, Recombinant Protein and Deoxycytidylate deaminase Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Thank you for your interest in spreading the word on Hypertension.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address. ...
The cloned 9.4-kb insert of plasmid pNHJ20L containing low-molecular-massnitrile hydratase (L-NHase) gene from Rhodococcus rhodochrous J1[Kobayashi, M. et al. (1991) Biochim. Biophys. Acta 1129, 23-33] wasdigested with various restriction enzymes, and the trimmed fragments wereinserted into pUC18 or pUC19. A 1.96-kb EcoRI-SphI region located 1.9-kbdownstream of the L-NHase gene was found to be essential for theexpression of amidase activity in Escherichia coli; the gene arrangementof the amidase and the NHase in R. rhodochrous J1 differed from those inRhodococcus species including N-774 and Pseudomonas chlororaphis B23. Thenucleotide-determined sequence indicated that the amidase consists of 515amino acids (54626 Da) and the deduced amino acid sequence of the amidasehad high similarity to those of amidases from Rhodococcus speciesincluding N-774 and P. chlororaphis B23 and to indole-3-acetamidehydrolase from Pseudomonas savastanoi. The amidase gene modified in thenucleotide sequence upstream ...
Mthfd1l - Mthfd1l (Myc-DDK-tagged ORF) - Rat methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (Mthfd1l), (10 ug) available for purchase from OriGene - Your Gene Company.
035301 (US EPA PC Code Text (Same as US EPA PC Code 600065)); 1689-84-5 (CAS number); 1689845; 1689845 (CAS number without hyphens); 2,6-Dibromo-4-cyanophenol; 2429 (CA DPR Chem Code) ); 3,5-Dibromo-4-hydroxybenzonitrile; 3,5-Dibromo-4-hydroxyphenyl cyanide; 4-Hydroxy-3,5-dibromobenzonitrile; 600065 (US EPA PC Code alt.); 600065 (US EPA PC Code Text (Same as US EPA PC Code 600065)); 729 (PDP Code); Benzonitrile, 3,5-dibromo-4-hydroxy-; Brittox; Brominal; Brominal Plus; Brominex; Bromoxinil; Bromoxinyl; Bromoxynil; Bromoxynil (ANSI); Bromoxynil phenol; BROMOXYNIL PHENOL (CA DPR Chem Code Text ); Bromoxynilphenol; Broxynil; Buctril; Chipco Buctril; Chipco Crab-Kleen; ENT 20852; M&B 10064; Nu-lawn weeder; Oxytril M; Yes (US EPA PC Code (Same as US EPA PC Code 600065)); Yes (US EPA PC Code (Same as US EPA PC Code 600065 ...
购买我们的重组人GTP cyclohydrolase 1蛋白。Ab114821为蛋白片段,在小麦胚芽中生产并经过ELISA, SDS-PAGE, Western blot实验验证。Abcam提供免费的实验方案,操作技巧及专业的支持。
Sack: Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin),Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin)]] [http://openwetware.org/images/8/84/Cell_Media_with_Gen_Selection_Agents_4-5-2012.pdf .],br ...
A distrofia muscular congênita (DMC) compõe um grupo de miopatias caracterizadas por hipotonia e fraqueza muscular notadas já no primeiro ano de vida. A forma de Ullrich é caracterizada por retrações musculares proximais e hiperextensibilidade distal. Cerca de 40% destes pacientes apresentam mutações em um dos genes que codificam as três sub-unidades do colágeno VI (COL6), acarretando deficiência total ou parcial na marcação da proteína. Analisamos, através de imunofluorescência, a marcação do COL6 em fragmentos musculares de 50 pacientes com DMC, 20 deles com ausência da marcação para merosina. Identificamos 4 casos com deficiência total da marcação do COL6 (8% do total), representando 13% dos casos com marcação normal para merosina. As alterações histológicas musculares dos pacientes com COL6 deficiente eram indistinguíveis das outras formas de DMC, porém mais brandas que as observadas na DMC com deficiência de merosina. Em três dos pacientes com COL6 ...
Learn more about Sífilis Congénita at Memorial Hospital DefiniciónCausasFactores de riesgoSíntomasDiagnósticoTratamientoPrevenció...
... IUPAC name Trichloromethane Other names Chloroform, Formyl trichloride, Methane trichloride, Methyl trichloride, Methenyl trichloride,
DI-fusion, le Dépôt institutionnel numérique de lULB, est loutil de référencementde la production scientifique de lULB.Linterface de recherche DI-fusion permet de consulter les publications des chercheurs de lULB et les thèses qui y ont été défendues.
Catalysis of the reaction: H2O + NAD+ + 2-aminomuconate semialdehyde = NADH + 2-amino-muconate. [EC:1.2.1.32, MetaCyc:1.2.1.32-RXN]
Disclosed is the preparation of novel difunctional cyanate monomers with increased aromatic chain length between the cyanate groups and thermosetting re...
Je to lov k, v jeho p tomnosti se prost bez zjevn ho d vodu nec t me dob e. Vn m me slabost, patn se n m d ch a jsme bezd vodn vy erpan a nerv zn . Jemu ale v na p tomnosti dob e je. Existuje n vod, jak se chr nit? Co kdy je to ivotn partner?
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இப்பக்கம் கடைசியாக 30 அக்டோபர் 2018, 01:37 மணிக்குத் திருத்தப்பட்டது ...
In enzymology, a N-succinylarginine dihydrolase (EC 3.5.3.23) is an enzyme that catalyzes the chemical reaction N2-succinyl-L-arginine + 2 H2O ⇌ {\displaystyle \rightleftharpoons } N2-succinyl-L-ornithine + 2 NH3 + CO2 Thus, the two substrates of this enzyme are N2-succinyl-L-arginine and H2O, whereas its 3 products are N2-succinyl-L-ornithine, NH3, and CO2. This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amidines. The systematic name of this enzyme class is N2-succinyl-L-arginine iminohydrolase (decarboxylating). Other names in common use include N2-succinylarginine dihydrolase, arginine succinylhydrolase, SADH, AruB, AstB, and 2-N-succinyl-L-arginine iminohydrolase (decarboxylating). This enzyme participates in arginine and proline metabolism. Schneider BL, Kiupakis AK, Reitzer LJ (1998). "Arginine catabolism and the arginine succinyltransferase pathway in Escherichia coli". J. Bacteriol. 180 (16): ...
Plasmid pSKB3.MPN348 from Dr. Sung-Hou Kims lab contains the insert methenyltetrahydrofolate synthetase and is published in Proteins. 2004 Sep 1. 56(4):839-43. This plasmid is available through Addgene.
29 December 2017 - Project Assistant Chemistry Jobs in NIT Jalandhar - Jalandhar. NITJ/CY/ 2017 /4766Project Assistant Chemistry Job Position in National Institute of Technology Jalandhar (NIT Jalandhar)
DD2 is 6 and very active, so weve been fighting a minor nit infestation for a few weeks. She wont sit still to be combed so it has been as much as
TY - BOOK. T1 - Enzymatic Nitrile reduction. AU - Klempier, Norbert. PY - 2011. Y1 - 2011. M3 - Other report. BT - Enzymatic Nitrile reduction. PB - .. ER - ...
MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersRiboflavin, FMN, and FADGTP cyclohydrolase II (TIGR00505; EC 3.5.4.25; HMM-score: 12.4) ...
We researched Park Tool Nitrile Gloves-Box of 100 deals, best reviews, and sales over the latter year for you at nitrile-gloves.
The Ja-panese TeX Devel-op-ment Com-mu-nity sub-mit-ted an up-date to the js-classes pack-age. Ver-sion num-ber: 2017-10-04 Li-cense type: bsd Sum-mary de-scrip-tion: Classes tai-lored for use with Ja-panese An-nounce-ment text ...
In a move aimed to make the state appear more financially stable, the Governor and some lawmakers are working on a bill that would move the states gold deposits from a Federal Reserve Bank in New York City to a secure location in Texas.
Background- Tetrahydrobiopterin (BH4) deficiency is reported to uncouple the enzymatic activity of endothelial nitric oxide synthase in diabetes mellitus. The mechanism by which diabetes actually leads to BH4 deficiency remains elusive. Here, we demonstrate that diabetes reduced BH4 by increasing 26S proteasome-dependent degradation of guanosine 5′-triphosphate cyclohydrolase I (GTPCH), a rate-limiting enzyme in the synthesis of BH4, in parallel with increased formation of both superoxide and peroxynitrite (ONOO−).. Methods and Results- Exposure of human umbilical vein endothelial cells to high glucose concentrations (30 mmol/L D-glucose) but not to high osmotic conditions (25 mmol/L L-glucose plus 5 mmol/L D-glucose) significantly lowered the levels of both GTPCH protein and BH4. In addition, high glucose increased both the 26S proteasome activity and the ubiquitination of GTPCH. Inhibition of the 26S proteasome with either MG132 or PR-11 prevented the high glucose-triggered reduction of ...
Thus, the two substrates of this enzyme are GTP and H2O, whereas its 3 products are formate, 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine, and diphosphate.. This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in cyclic amidines. The systematic name of this enzyme class is GTP 7,8-8,9-dihydrolase (diphosphate-forming). Other names in common use include guanosine triphosphate cyclohydrolase II, and GTP-8-formylhydrolase. This enzyme participates in riboflavin metabolism.. ...
TY - JOUR. T1 - Identification of novel bacterial histidine biosynthesis inhibitors using docking, ensemble rescoring, and whole-cell assays. AU - Henriksen,Signe Teuber. AU - Liu,J.. AU - Estiu,G.. AU - Oltvai,Z.N.. AU - Wiest,O.. PY - 2010. Y1 - 2010. N2 - The rapid spread on multidrug-resistant strains of Staphylococcus aureus requires not just novel treatment options, but the development of faster methods for the identification of new hits for drug development. The exponentially increasing speed of computational methods makes a more extensive use in the early stages of drug discovery attractive if sufficient accuracy can be achieved. Computational target identification using systems-level methods suggested the histidine biosynthesis pathway as an attractive target against S. aureus. Potential inhibitors for the pathway were identified through docking, followed by ensemble rescoring, that is sufficiently accurate to justify immediate testing of the identified compounds by whole-cell assays, ...
Buy our Recombinant Human APOBEC1 protein. Ab116768 is a protein fragment produced in Wheat germ and has been validated in WB, ELISA, SDS-PAGE. Abcam provides…
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
An anatomically correct mannequin representing a 4 lb, 16 inch long female baby developed to review the principles, skills and tools necessary for insertion, assessment, dressing care, securement and maintenance of vascular access devices (VADs) in infants.
Acrodermatite enteropática ocorre de duas formas:forma hereditária autossômica recessiva e forma adquirida. A forma congênita de AE é desordem genética rara caracterizada por defeito congênito da absorção gastrintestinal de zinco. Manifestações da doença tipicamente apresentam-se quando o infante afetado é retirado da amamentação. A forma adquirida é decorrente de deficiência nutricional de zinco, ou seja, em infantes prematuros que recebem alimentação parenteral prolongada. As lesões cutâneas características incluem erupção vesiculobolhosa periorificial e de extremidades levando a placas crostosas intensamente demarcadas e descamativas. Na fase aguda, irritabilidade e distúrbios emocionais são evidentes devido à destruição (atrofia) do córtex cerebral.. ...
Genetic editing of HBV DNA by monodomain human APOBEC3 cytidine deaminases and the recombinant nature of APOBEC3G.. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
The effects of adrenalectomy, acute acidosis and treatment with adrenal steroids upon the activities of renal glutaminase, asparaginase, guanase and adenosine deaminase were studied in the rat. None of the procedures or treatments employed affected significantly the in vitro activities of renal glutaminase, asparaginase and guanase. Pretreatment of rats with hydrocortisone or aldosterone resulted in the increased activity of renal adenosine deaminase.. ...
A perfluoroalkylene oxide dinitrile, an aromatic dinitrile N-oxide and an aromatic trinitrile are terpolymerized to obtain a poly(perfluoroalkylene oxide) oxadiazole containing pendent aromatic nitrile groups. The fluorinated polymer product can be cured with a poly-functional nitrile N-oxide to provide elastomers that are particularly useful in aircraft applications involving use temperatures ranging from about -70F to about 400F. For example, the elastomers can be employed as fuel tank sealants, coatings, O-ring seals, diaphragms, and the like.*Patents
Information about the open-access journal Faslnāmah-i Pizhūhish/Nāmah-i Iqtisādī in DOAJ. DOAJ is an online directory that indexes and provides access to quality open access, peer-reviewed journals.
Hygromycin B is an aminoglycosidic antibiotic that inhibits protein synthesis by disrupting translocation and promoting mistranslation at the 80S ribosome. Because it uses a different mode of action than Geneticin®, Blasticidin S, or Zeoci
The Genetics Society of America (GSA), founded in 1931, is the professional membership organization for scientific researchers and educators in the field of genetics. Our members work to advance knowledge in the basic mechanisms of inheritance, from the molecular to the population level.. Online ISSN: 1943-2631. ...
Learn more about Síndrome de Rubéola Congénita at Reston Hospital Center DefiniciónCausasFactores de riesgoSíntomasDiagnósticoTratamientoPrevenció...
Amidases catalyse the hydrolysis of amides to the corresponding carboxylic acid and ammonia. They exist in all kingdoms of the living world but have been most extensively characterised amongst the bacteria.. A number of studies of amidase classification (10.1016/S0167-4838(96)00145-8)(10.1046/j.1365-2672.2001.01378.x)(10.1186/gb-2001-2-1-reviews0001) have revealed that the bacterial aliphatic amidases (broadly classed as acylamide amidohydrolase, EC 3.5.1.4) are made up of two types.. The first group, the nitrilase-related family, includes the aliphatic amidases, hydrolysing only short-chain aliphatic amides (10.1046/j.1365-2672.2001.01378.x). The enzymes are typically homohexamers of approximately 230kDa, and contain a Cys166 residue (Pseudomonas aeruginosa amidase numbering), conserved across both nitrilase and amidase. This residue is believed to act as the catalytic nucleophile. The amidases from P. aeruginosa (10.1016/0014-5793(87)80163-1)(10.1016/j.ijbiomac.2003.08.002[c/ite], Rhodococcus ...
Search the history of over 284 billion web pages on the Internet. new post here Search the history of over 284 billion web pages on the Internet. Mn 0 01 051 1 10 100 10th 11 11d0003 12 13 14 141a 143b 15 16 17 17igp 18 19 1900 1901 1902 1903 1904 1905 1906 1907 1908 1909 1910 1911 1912 1913 1914. Mn 0 01 051 1 10 100 10th 11 11d0003 12 13 14 141a 143b 15 16 17 17igp 18 19 1900 1901 1902 1903 1904 1905 1906 1907 1908 1909 1910 1911 1912 1913 1914. Mn 0 01 051 1 10 100 10th 11 11d0003 12 13 14 141a 143b 15 16 17 17igp 18 19 1900 1901 1902 1903 1904 1905 1906 1907 1908 1909 1910 1911 1912 1913 1914. Search the history of over 284 billion web pages on the Internet. Mn 0 01 051 1 10 100 10th 11 11d0003 12 13 14 141a 143b 15 16 17 17igp 18 19 1900 1901 1902 1903 1904 1905 1906 1907 1908 1909 1910 1911 1912 1913 1914. essay skills development act Search the history of over 284 billion web pages on the Internet. Mn 0 01 051 1 10 100 10th 11 11d0003 12 13 14 141a 143b 15 16 17 17igp 18 19 1900 1901 1902 ...
In this study, we have for the first time demonstrated that ONOO− releases zinc from GTPCH1 and that zinc removal lowers GTPCH1 activity. Further, zinc-deleted GTPCH1 exhibits increased ubiquitination and reduced stability. We found that ONOO− generated by high glucose suppresses GTPCH1 activity along with increased ubiquitination and destruction of this enzyme. Finally, GTPCH1 ubiquitination and destruction is markedly increased in parallel with enhanced ONOO− in STZ-induced diabetic mice in vivo. Overall, our results suggest that ONOO− releases zinc, inhibits GTPCH1 activity, and increases GTPCH1 ubiquitination.. The major finding of this study is that ONOO− removes the zinc in GTPCH1, resulting in enzyme inhibition. Several lines of evidence are consistent with the hypothesis that loss of zinc by ONOO− oxidation underlies the inactivation of GTPCH1. First, ONOO− dose-dependently releases zinc from GTPCH1 (Fig. 1B). Second, the effects of ONOO− are mimicked by TPEN, a selective ...
Federal Register: May 13, 1998 (Volume 63, Number 92)] [Rules and Regulations] [Page 26473-26481] >From the Federal Register Online via GPO Access [wais.access.gpo.gov] [DOCID:fr13my98-38] ----------------------------------------------------------------------- ENVIRONMENTAL PROTECTION AGENCY 40 CFR Part 180 [OPP-300661; FRL-5790-8] RIN 2070-AB78 Bromoxynil; Pesticide Tolerance AGENCY: Environmental Protection Agency (EPA). ACTION: Final rule. ----------------------------------------------------------------------- SUMMARY: This regulation establishes tolerances for bromoxynil and DBHA in or on cotton. In addition, this regulation establishes tolerances for bromoxynil and DBHA in or on meat, meat by products, and fat of cattle, hogs, horses, goats, and sheep. Further, this regulation establishes tolerances for bromoxynil and DBHA in milk, eggs, and poultry meat, meat by-products, and fat. Rhone-Poulenc Ag Company requested the tolerances for cotton under the Federal Food, Drug, and Cosmetic Act, ...

GTP cyclohydrolase II - WikipediaGTP cyclohydrolase II - Wikipedia

Aminohydrolases. *Guanine deaminase. *Adenosine deaminase. *AMP deaminase. *Inosine monophosphate synthase. *DCMP deaminase ...
more infohttps://en.wikipedia.org/wiki/GTP_cyclohydrolase_II

tenA - Aminopyrimidine aminohydrolase - Staphylococcus haemolyticus (strain JCSC1435) - tenA gene & proteintenA - Aminopyrimidine aminohydrolase - Staphylococcus haemolyticus (strain JCSC1435) - tenA gene & protein

Aminopyrimidine aminohydrolaseBy similarity. Manual assertion inferred from sequence similarity toi ... sp,Q4L7X6,TENA_STAHJ Aminopyrimidine aminohydrolase OS=Staphylococcus haemolyticus (strain JCSC1435) OX=279808 GN=tenA PE=3 SV= ...
more infohttps://www.uniprot.org/uniprot/Q4L7X6

Aminohydrolase - WikipediaAminohydrolase - Wikipedia

An aminohydrolase is a hydrolase enzyme which acts upon an amino group. Aminohydrolases are classified under EC number EC 3.5.4 ... Aminohydrolases at the US National Library of Medicine Medical Subject Headings (MeSH) Molecular and Cellular Biology portal. ...
more infohttps://en.wikipedia.org/wiki/Aminohydrolase

ssnA - Putative aminohydrolase SsnA - Escherichia coli (strain K12) - ssnA gene & proteinssnA - Putative aminohydrolase SsnA - Escherichia coli (strain K12) - ssnA gene & protein

Putative aminohydrolase SsnAAdd BLAST. 442. Proteomic databases. PaxDb, a database of protein abundance averages across all ... IPR017700. Aminohydrolase_SsnA. IPR011059. Metal-dep_hydrolase_composite. IPR032466. Metal_Hydrolase. Pfam protein domain ... IPR017700. Aminohydrolase_SsnA. IPR011059. Metal-dep_hydrolase_composite. IPR032466. Metal_Hydrolase. Pfam protein domain ... sp,Q46812,SSNA_ECOLI Putative aminohydrolase SsnA OS=Escherichia coli (strain K12) OX=83333 GN=ssnA PE=1 SV=2 ...
more infohttp://www.uniprot.org/uniprot/Q46812

Aminopyrimidine aminohydrolase - WikipediaAminopyrimidine aminohydrolase - Wikipedia

Aminopyrimidine aminohydrolase (EC 3.5.99.2, thiaminase, thiaminase II, tenA (gene)) is an enzyme with systematic name 4-amino- ... Aminopyrimidine aminohydrolase at the US National Library of Medicine Medical Subject Headings (MeSH) Molecular and Cellular ... 5-aminomethyl-2-methylpyrimidine aminohydrolase. This enzyme catalyses the following chemical reaction (1) 4-amino-5- ...
more infohttps://en.wikipedia.org/wiki/Aminopyrimidine_aminohydrolase

Domain combinations for N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily in Mycobacterium tuberculosis KZN...Domain combinations for N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily in Mycobacterium tuberculosis KZN...

Domain architectures illustrate each occurrence of the N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily. ... Domain combinations containing the N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily in Mycobacterium ... Home > Genomes > Mycobacterium tuberculosis KZN 1435 > N-terminal nucleophile aminohydrolases (Ntn hydrolases) > Domain ... 7 domain combinations include a N-terminal nucleophile aminohydrolases (Ntn hydrolases) domain in Mycobacterium tuberculosis ...
more infohttp://supfam.cs.bris.ac.uk/SUPERFAMILY/cgi-bin/allcombs.cgi?genome=1R

Manganese in the structure of Crystal Structure of (S)-Ureidoglycine Aminohydrolase From Arabidopsis Thaliana in Complex With...Manganese in the structure of Crystal Structure of (S)-Ureidoglycine Aminohydrolase From Arabidopsis Thaliana in Complex With...

Ureidoglycine Aminohydrolase From Arabidopsis Thaliana in Complex With Its Substrate, (S)-Ureidoglycine ... The binding sites of Manganese atom in the structure of Crystal Structure of (S)-Ureidoglycine Aminohydrolase From Arabidopsis ... Manganese in the structure of Crystal Structure of (S)-Ureidoglycine Aminohydrolase From Arabidopsis Thaliana in Complex With ...
more infohttp://manganese.atomistry.com/pdb4e2s.html

Succinyl-diaminopimelate desuccinylase - WikipediaSuccinyl-diaminopimelate desuccinylase - Wikipedia

Aminohydrolases. *Guanine deaminase. *Adenosine deaminase. *AMP deaminase. *Inosine monophosphate synthase. *DCMP deaminase ...
more infohttps://en.wikipedia.org/wiki/Succinyl-diaminopimelate_desuccinylase

PAJ051Hu01 | Polyclonal Antibody to Guanine Deaminase (GDA) | Homo sapiens (Human) USCN(Wuhan USCN Business Co., Ltd. )PAJ051Hu01 | Polyclonal Antibody to Guanine Deaminase (GDA) | Homo sapiens (Human) USCN(Wuhan USCN Business Co., Ltd. )

Guanine aminohydrolase; p51-nedasin , Products for research use only! ... CYPIN; Guanase; Guanine aminase; Guanine aminohydrolase; p51-nedasin. *Enzyme & Kinase. *KO-Validated ...
more infohttp://www.uscnk.com/uscn/Polyclonal-Antibody-to-Guanine-Deaminase-

Beta-lactamase - wikidocBeta-lactamase - wikidoc

Aminohydrolases. *Guanine deaminase. *Adenosine deaminase. *AMP deaminase. *Inosine monophosphate synthase. *DCMP deaminase ...
more infohttp://wikidoc.org/index.php/Beta-lactamase

Thiaminase - wikidocThiaminase - wikidoc

Aminohydrolases. *Guanine deaminase. *Adenosine deaminase. *AMP deaminase. *Inosine monophosphate synthase. *DCMP deaminase ...
more infohttps://www.wikidoc.org/index.php/Thiaminase

AMPD3 Gene - GeneCards | AMPD3 Protein | AMPD3 AntibodyAMPD3 Gene - GeneCards | AMPD3 Protein | AMPD3 Antibody

Complete information for AMPD3 gene (Protein Coding), Adenosine Monophosphate Deaminase 3, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
more infohttps://www.genecards.org/cgi-bin/carddisp.pl?gene=AMPD3

Deaminase | definition of deaminase by Medical dictionaryDeaminase | definition of deaminase by Medical dictionary

Also called aminohydrolase. deaminase. An ENZYME that brings about the breakdown of amino compounds.. deaminase. an enzyme ...
more infohttps://medical-dictionary.thefreedictionary.com/deaminase

Anti-CDA antibody (ab82347) | AbcamAnti-CDA antibody (ab82347) | Abcam

Rabbit polyclonal CDA antibody validated for WB, ELISA, ICC/IF and tested in Human and Mouse. Referenced in 1 publication and 1 independent review. Immunogen…
more infohttps://www.abcam.com/cda-antibody-ab82347.html

KEGG ENZYME: 3.5.5.7KEGG ENZYME: 3.5.5.7

aliphatic nitrile aminohydrolase. Reaction(IUBMB). R-CN + 2 H2O = R-COOH + NH3 [RN:R00540]. ...
more infohttp://www.genome.jp/dbget-bin/www_bget?ec:3.5.5.7

Adenosine Deaminase/ADA Antibody (NBP1-77775): Novus BiologicalsAdenosine Deaminase/ADA Antibody (NBP1-77775): Novus Biologicals

Adenosine aminohydrolase. *Adenosine Deaminase. *EC 3.5.4.4. Limitations. This product is for research use only and is not ...
more infohttps://www.novusbio.com/products/adenosine-deaminase-ada-antibody_nbp1-77775

Dexrazoxane (Professional Patient Advice) - Drugs.comDexrazoxane (Professional Patient Advice) - Drugs.com

Hydrolyzed by dihydropyrimidine aminohydrolase and dihydroorotase. Excretion. Urine (42%) Half-Life Elimination. 2.1 to 2.5 ...
more infohttps://www.drugs.com/ppa/dexrazoxane.html

ADA Gene - GeneCards | ADA Protein | ADA AntibodyADA Gene - GeneCards | ADA Protein | ADA Antibody

Adenosine aminohydrolase (ADA_HUMAN). *Mutant adenosine deaminase (Q16073_HUMAN). Graphical View of Domain Structure for ...
more infohttps://www.genecards.org/cgi-bin/carddisp.pl?gene=ADA

KEGG ENZYME: 3.5.4.38KEGG ENZYME: 3.5.4.38

single-stranded DNA cytosine aminohydrolase. Reaction(IUBMB). cytosine in single-stranded DNA + H2O = uracil in single-stranded ...
more infohttps://www.genome.jp/dbget-bin/www_bget?enzyme+3.5.4.38

EC 3.5.4.33EC 3.5.4.33

Systematic name: tRNA(adenine34) aminohydrolase. Comments: The enzyme is involved in editing of tRNA. The active site contains ...
more infohttps://www.qmul.ac.uk/sbcs/iubmb/enzyme/EC3/5/4/33.html

Human Adenosine Deaminase: Stoichiometry of the Large form Complex | SpringerLinkHuman Adenosine Deaminase: Stoichiometry of the Large form Complex | SpringerLink

... adenosine aminohydrolase, ADA, EC 3.5.4.4.) is widely distributed in human tissues. In some tissues ADA exists exclusively as ... Adenosine deaminase (adenosine aminohydrolase, ADA, EC 3.5.4.4.) is widely distributed in human tissues. In some tissues ADA ...
more infohttps://link.springer.com/chapter/10.1007/978-1-4684-8559-2_30

Identification of candidate genes controlling oil content by combination of genome-wide association and transcriptome analysis...Identification of candidate genes controlling oil content by combination of genome-wide association and transcriptome analysis...

Background Increasing seed oil content is one of the most important targets for rapeseed ( Brassica napus) breeding. However, genetic mechanisms of mature seed oil content in Brassica napus ( B. napus...
more infohttps://rd.springer.com/article/10.1186%2Fs13068-019-1557-x

Chen - Leibniz Universität HannoverChen - Leibniz Universität Hannover

RNA Methylation in Plants and Man: The Catabolism of modified Nucleotides from RNA decay by N6-methy-AMP Aminohydrolase.. ... We identified and partially characterized a novel enzyme, N6-methyl-AMP (N6-mAMP) aminohydrolase, which clears the methyl ...
more infohttps://www.uni-hannover.de/de/forschung/wiss-nachwuchs/postdocs/bisher-gefoerderte-projekte/wif-ii-projekte-2017/chen/